LOCUS       DASTKC010000001         7822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_710355, whole genome shotgun sequence.
ACCESSION   DASTKC010000001 DASTKC010000000
VERSION     DASTKC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710355"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VFR25_00005"
     CDS             <1..803
                     /locus_tag="VFR25_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9939458.1"
                     /translation="RADKPFVAINCAALPEQLLESELFGHEKGAFTGAIATKIGRLEQ
                     AAGGTLFLDEIAEMSPVLQAKFLRVLEEREFQRVGGTRTIKADVRVIAATNRDLPTSI
                     AKQTFREDLYYRLNVFPIHIAPLRNRREDVLPLAEAFLEDLGRSMGRPAAGISRDARE
                     WLLAHPWPGNVRELRNAIERAILMSDGGLITHDHFPAIPARVEAGLAHTNGSTPPQTT
                     NGMNLDHAERGLVERALVETKGNKSKAARLLGLSRAQLYWRLEKHGLQ"
     gene            1071..1307
                     /locus_tag="VFR25_00010"
     CDS             1071..1307
                     /locus_tag="VFR25_00010"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HET9939459.1"
                     /translation="MSQPNVERVIGVLVTDERLRAQFTRDPRSALLDLVRKGMDLNPT
                     ELWSLACLDPSELARFAQAIDARLQKADLEVEPQ"
     gene            1304..2836
                     /locus_tag="VFR25_00015"
     CDS             1304..2836
                     /locus_tag="VFR25_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9939460.1"
                     /translation="MKLRFLTLALAAGLLAPAPTRAADHAITLGEAVRLAIQKNEGLV
                     SARESAGAARGTLKSANGAYDPVLYGDGTWQRVKQPTNSSIPGATLAPENESSEGGLE
                     LRQYLPTGGTLSLKGRGGRETDQGFVLLSPAYLTRVGAEFRQPLLRNRSIDPARRDIK
                     VAKAGRDAAEASLDQATIETLAAVERAYWTLVDARLDIGVRERAVELAQEQLTETQHR
                     VDTGSVPRTELSQPTAELARRRGDLLEATEARARAQNALKLLILAGSGDPMWDSDLAP
                     VDDSAVTVAPVDIAAEMELALKNRPELAVAQAIVDQRRTETSFAKDGRWPSLDAVLSY
                     DRFGIAGDPAVPPGEVAPDLQGKWGDSFQSLTDGDFDAARVALELELPIVNRAAKGAT
                     EAASSRERQARAELELVRKLIRAEVLDAAAALETAGQRIEAAKAGREAAEVQLSAEQD
                     RYASGLSTNFLVLTRQNDLSRAQLDEISSLTDYRAARTEMARATGRLAEEYGIDTSDT
                     QR"
     gene            2850..3974
                     /locus_tag="VFR25_00020"
     CDS             2850..3974
                     /locus_tag="VFR25_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9939461.1"
                     /translation="MKKRMIIVVAALVVFFVAIASVKFLQIRAAIAQYANWQQPPEAV
                     TTIVTHEEPWAASLTAIGSVEAVHGVTVSADLPGIVEAVNIDSGRRVGAGDVLVRQDT
                     RQEQAQLDAAESQRDLANLNLARGRQLLDKGVVAQAEFDRLDAEAKQAEARVGEIRAT
                     IDRKRIRAPFSGVLGIRQVNLGQYLKGGDPVVPLQSMDPIYVNFALPQGDVAALKLGA
                     EVTVTADSVAGVFAGRVTAINAVVDQATRNVMVQATFRNLSGSLRPGMYVDVQASRGS
                     AQQVIALPASAVSYAPYGNSVFVVSQLKDPKGKTYAGVSQRFVKLGAGRGDQVAVVSG
                     LKPGEEIVTSGVFKLRNGAAVFVNNKVIPSNNPTPKPEDS"
     gene            3976..7077
                     /locus_tag="VFR25_00025"
     CDS             3976..7077
                     /locus_tag="VFR25_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9939462.1"
                     /translation="MKFTDLFVKRPVLAMVVNLVILIAGLQSIRSLSVRQYPRSDMAV
                     ITVSTAYVGANADLVRGFITTPLERVIASADGIDYIESSSAQGVSTITAHLKLNYDTN
                     AALTQVQAKVAQVRNDLPPEAEAPIINIETADNRFAAMYLGFSSKDLDQNQITDYLTR
                     VVQPKLSAIEGVQRADILGGRTFAMRIWLKPDLMAARGISPSDVHDALADNNYLAAVG
                     STKGSMVSVNLVANTDLQTPDEFRQLVVKEDKGNVVRLGDIADVSLGAENYEQDVRFD
                     GQGATFMGIWVLPTANTLEVIHRVREALPGIEAQLPVGMKAGIPYDSTEYIESAIHEV
                     LKTLTETLLIVVLVIFLFLGSVRGVIIPVVAIPISLIGAVFLMAAAGFTINLLTLLAI
                     VLSVGLVVDDAIVMVENVERHLRAGSKPMQAAIDAARELTGPIIAMTITLAAVYTPVA
                     IQGGLTGSLFREFAFTLAGAVVVSGVVALTLSPMMGSRLLRAGDDEKGFAGWINRRFD
                     RVRDGYKDALASTLRYRPAVYVVWAFVVLLIPPFYLFSQRELAPREDPGVVFGIIQGS
                     ANSTLDQTRLFTTAVNDVYQSFPETKNTFQLTFPTGGFGGMVTKPWGDRKKNTAQLQV
                     EAAAQFSKIPGIRVISLVPPPLPGGGDFPVDLVIASTAEPQQLIEFSNQLVQKAYASG
                     LFMFADADLKYDQPQAEVVFNRDQVRSQGVELSQAGKDLSTLLGGNYVNRFSIQGRSY
                     KVIPQVKRTERLTPDQLENMYVTGTDGGLVPLSTFASIQSSTQPRELKRFQQLNAVRI
                     QGVVPPNVSLDQALTYLEGEAKKILPQGYTIDYAGESRQLRTEGSRFLGIFLLSAVLI
                     FLVLAAQFESFRDPFVILAGSAPLAVSGALLFSFLGFTSLNIYSQVGLITLVGLVAKN
                     GILIVQFANQLRETGIAKLEAVIEASGTRLRPILMTTAATVFGHLPLIFASGPGSGAR
                     NSIGIMLVTGMIIGTAFTLFVVPSIYMLVAHAQERVAVREKEAAPGDLGEAPEEIPAV
                     ATV"
     gene            7153..>7822
                     /locus_tag="VFR25_00030"
     CDS             7153..>7822
                     /locus_tag="VFR25_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939463.1"
                     /translation="MLPTTRILTAMLIAAAALTIPGPPAATAADLKKVYGAALGGDMA
                     RALAILDSIDVSQLSPKDSTARECLKRTFGNPPVHEDLPPKSGQILDAYRTYWQTAML
                     RRAPQADAERTLLGTLRSILAIAPTDTARLDDVSERARLAIAGERLFALTGVTLPYYE
                     LMIWRTQEPTTYHVKLPERTIEVHVVFLDDFVSLGWAGYATCGRAHSGGWATQDSLFA
                     LKSAY"
BASE COUNT         1351 a         2616 c         2601 g         1254 t
ORIGIN      
        1 cgcgcgcgga caagccgttc gtcgccatca actgcgccgc gcttccggag cagctcctcg
       61 agtcggagct cttcgggcac gaaaagggcg cattcacggg tgccatcgcg accaagatcg
      121 gacgactcga gcaggctgcg ggcggcacgc tcttcctcga cgagatcgcg gagatgagcc
      181 ccgtccttca agcgaagttc ctccgggtgc tcgaggagcg tgaattccag cgggtcgggg
      241 gcacgcgcac gatcaaggcg gacgtgcgcg tgatcgcggc gacgaaccgg gatctgccca
      301 cgagcatcgc gaagcagacc ttccgggagg atctgtacta ccggctcaat gtctttccga
      361 tccacatcgc cccgcttcgg aatcgcaggg aggacgtcct gccgctggcc gaggccttcc
      421 tcgaggatct gggacgctcg atggggcgcc ctgccgcggg catctcgcgc gacgcgcgcg
      481 agtggctcct ggcgcatccg tggccgggca acgtgcgcga gcttcgaaac gcgatcgaac
      541 gcgccatctt gatgagtgac ggcgggctga tcacgcacga tcacttcccg gcgattccgg
      601 cccgcgtcga ggcgggcctg gcgcacacga acggctcgac tccgccccag actacgaatg
      661 gcatgaacct cgaccacgcg gagcgcggcc tcgtggagcg tgcgctcgtc gagacgaagg
      721 ggaacaaatc caaggccgcc cgtctcctcg gcctgagccg cgcgcagctc tactggcgcc
      781 tggagaagca cggcctccag tagctgatac gccgtccttc tgaacgcctg tcgatgccat
      841 cggcaggcct tttgtttttc gtagcgtcca ccaagcctct gtagagttcc gcgactgcag
      901 tcgtgtcgac agcttgtcga tgcatcgaca ctgtcgatcc ctccacgcaa acggcgccac
      961 tcccatctgt ccatgcaaca tctcgccgcg caacgacctc cacgggatcg cactgcgttc
     1021 gcctccatgg cacgtccctt gagataggga acagccaaag gaggaacgcc atgagccaac
     1081 cgaatgtgga acgcgtgatc ggtgtcctcg taaccgacga gcggttgcgg gcgcagttca
     1141 cgcgcgatcc gagatccgcg ctgctcgacc tggtgcggaa gggaatggac ctgaatccga
     1201 cggagctctg gtcgcttgcg tgcctcgatc ccagcgagct ggcgcgcttc gcccaagcca
     1261 tcgatgcgcg gctgcaaaag gccgatctgg aggtggaacc ccaatgaagc tgagattcct
     1321 gaccctggcc cttgcggcgg gcctgctggc tcccgcgccc acccgagccg ccgatcacgc
     1381 gatcacgctc ggagaggccg tgcggctcgc gattcagaag aacgaaggcc tcgtgagcgc
     1441 ccgcgaatcg gcgggtgcgg cgcggggaac gctcaagagt gcgaacggcg cctacgatcc
     1501 cgtgctctac ggggacggaa cgtggcaacg ggtcaagcag ccgacgaact catccatccc
     1561 gggcgccacg cttgctcccg agaacgaatc gtccgaaggc ggcctcgagc ttcgccagta
     1621 tctgccgacc gggggcacgc tgtcgctgaa agggcgcggc ggtcgggaaa ccgatcaggg
     1681 cttcgtgctc ctgtcgcccg cctacctgac ccgagtgggc gccgaatttc gccagccgct
     1741 gcttcggaac cgctcgatcg atccggcgag acgcgacatc aaggtcgcga aggccggccg
     1801 ggacgcggcg gaggcgtcgc tcgaccaggc gacgatcgag accctggcgg cggtcgagcg
     1861 ggcgtactgg acgctcgtgg acgcgcgtct cgacatcggt gtcagggagc gcgcggtcga
     1921 gcttgcccag gagcagctga ccgagaccca gcatcgcgtc gataccggat cggtcccgcg
     1981 caccgagctg tctcagccca cggccgagct cgcgcggcgg cgcggtgacc tcctcgaggc
     2041 gaccgaggct cgtgcccgcg cccagaacgc gctcaagctc ctgatcctcg ccggttccgg
     2101 cgatcccatg tgggactcgg atctggcgcc ggtcgacgac tcggccgtca cggtcgcgcc
     2161 ggtcgacatc gcggccgaga tggagctcgc actcaagaac aggccggagc tcgccgtcgc
     2221 gcaagcgatc gtggaccagc gccggaccga gacgtccttc gcgaaggacg gacgctggcc
     2281 gagtctggac gcggtcctct cctacgaccg gttcggcatc gcgggagacc cggccgttcc
     2341 tccaggcgag gtcgcgccgg atcttcaggg gaagtggggc gactccttcc agtcgctcac
     2401 cgatggcgac ttcgacgcgg cacgcgtcgc gctcgagctg gagctcccga tcgtgaaccg
     2461 cgccgccaag ggggcgacgg aagcggcgag cagtcgcgag cgccaagctc gggccgagct
     2521 cgagctggtc cggaagttga tccgcgccga ggtgctggac gccgcggcgg cgctcgagac
     2581 cgccgggcag cgcatcgagg ccgccaaggc ggggcgcgag gctgcggagg tgcagctctc
     2641 ggcggagcag gatcggtacg ccagcgggct ctccacgaac ttcctggtgc tcacgcgtca
     2701 gaacgacttg tcccgtgccc agctcgacga gatctcgtcc ctcaccgact accgcgcggc
     2761 gcgcaccgag atggcgcgcg ccacggggcg gctggccgag gaatacggaa tcgacacgag
     2821 cgacacccaa cgatagagga ggtgctctca tgaagaagcg aatgatcatc gtggtcgcgg
     2881 ccctcgtggt cttcttcgtc gcgatcgcgt ccgtcaagtt cctccagatc cgcgcggcca
     2941 tcgcccagta cgccaactgg cagcagccgc cggaagcggt cacgaccatc gtgacccatg
     3001 aggaaccgtg ggccgcgtcg ctcaccgcga tcggcagcgt ggaggcggtc cacggtgtca
     3061 ccgtgagcgc ggacctgccg ggcatcgtcg aggccgtgaa catcgactcc ggtcggcgcg
     3121 tcggcgccgg ggacgtgctg gtgcggcagg acacgcgcca ggaacaagcc cagctcgacg
     3181 cggcggagtc gcagcgcgat ctcgccaacc tcaacctcgc acgcgggcgt cagctcctcg
     3241 acaagggcgt cgtggcgcag gcggagttcg accgactcga tgccgaagcc aagcaggcgg
     3301 aggcgcgcgt cggtgagatc cgcgcgacga tcgaccggaa gcgaatccgc gcgccgttct
     3361 cgggcgtcct cggcatacgc caggtgaacc tcggccagta cctgaaaggc ggggaccccg
     3421 tggtgccgct ccagtccatg gatcccattt atgtgaactt cgcgctcccg cagggcgatg
     3481 tcgcggccct caagctcggt gccgaggtga cggtgacggc cgacagcgtg gcgggcgtct
     3541 tcgcgggtcg ggtcacggcc atcaacgccg tggtggatca ggcgacccga aacgtcatgg
     3601 tccaggcgac gttccgaaat ctcagtggct ccctccgccc gggcatgtac gtcgacgtcc
     3661 aggcgagccg ggggtcggcg cagcaggtga tcgccctccc agcctcggcg gtgagctacg
     3721 cgccctatgg caactcggtc ttcgtcgtca gccagctcaa ggatccgaag gggaagacct
     3781 acgccggcgt gtcgcagcgc ttcgtgaagc tcggcgccgg ccgcggcgac caggtggccg
     3841 tcgtctccgg actcaagccg ggggaggaaa tcgtcacctc cggcgtgttc aagctccgaa
     3901 acggcgccgc cgtgttcgtg aacaacaagg tcattccgtc caacaatccc acgccgaagc
     3961 cggaggacag ctgagatgaa attcaccgac ctgttcgtca agcggccggt cctggcgatg
     4021 gtcgtcaatc tggtcattct catcgccggg ctccagtcga tccgctccct cagcgtccgg
     4081 cagtatccgc ggagtgacat ggcggtcatc acggtctcga cggcgtacgt cggggccaac
     4141 gccgatctcg tgcgaggctt catcacgaca ccgctcgagc gagtgatcgc gagcgcggac
     4201 ggcatcgatt acatcgagtc gtcgagcgct cagggcgtga gcacgatcac ggcccacttg
     4261 aagctcaact acgatacgaa cgcggccctt actcaggtcc aggccaaggt ggcccaggtt
     4321 cgaaacgacc tgccccccga ggccgaggcg ccgatcatca acatcgagac cgcggacaac
     4381 cggttcgcgg cgatgtacct cgggttctcg tccaaggatc tcgaccagaa ccagatcacg
     4441 gactacctga cccgggtggt gcagcccaag ctgagcgcga tcgagggcgt gcagcgggcc
     4501 gatatcctcg gcgggcgcac gttcgccatg cgcatctggc tcaagccgga tctcatggca
     4561 gcccgcggga tctcgccgtc ggacgtccac gacgctctgg cggacaacaa ctacctggcc
     4621 gcggtcggga gcacgaaggg ctccatggtc tccgtgaacc tcgtggccaa cacggacctc
     4681 cagactccgg acgagttccg ccagctcgtg gtgaaggagg acaagggaaa cgtcgtgcgg
     4741 ctgggcgaca tcgccgacgt gtccctgggg gcggagaact acgagcagga cgtccgcttc
     4801 gacgggcagg gggcgacgtt catggggatc tgggtgctcc caaccgcgaa cacgctcgag
     4861 gtgatccacc gcgtgcgcga ggcgctgccg ggcatcgagg ctcagcttcc ggtcgggatg
     4921 aaggctggga tcccgtacga ctcgaccgag tacatcgaga gcgccatcca cgaggtgctg
     4981 aagaccctca ccgagacgct cctcatcgtg gtcctcgtca tcttcctgtt cctgggatcc
     5041 gtgcgcggcg tgatcatccc ggtcgtggcc attccgatct ccttgatcgg agctgtgttc
     5101 ctgatggcgg ccgccggatt cacgatcaac ctcctgaccc tgctcgcgat cgtgctctcg
     5161 gtggggctcg tggtcgacga cgcgatcgtc atggtcgaga acgtcgagcg acatctccgc
     5221 gccggctcga aaccgatgca ggcggcgatc gacgccgccc gggagctgac cggcccgatc
     5281 atcgccatga cgatcaccct ggccgcggtc tatacgccgg tcgcgattca ggggggcctc
     5341 acgggatcgc tgttccgcga gttcgcgttc acgctcgcgg gcgctgtcgt ggtgtcgggt
     5401 gtcgtcgcgc tcacactgtc cccgatgatg ggatcgcggc tcctccgcgc cggcgacgac
     5461 gaaaagggct tcgcggggtg gatcaatcgc cggttcgatc gtgtccgcga cggttacaaa
     5521 gacgcactcg cgtcgaccct ccggtatcgc ccggcggtct acgtcgtgtg ggcgttcgtg
     5581 gtgctcctga tcccgccgtt ctacctgttc tcgcagcggg agctggcgcc gcgcgaggat
     5641 ccgggggtcg tgtttgggat catccagggg tcggccaact cgacgctcga ccagacgcgt
     5701 cttttcacaa cggccgtcaa cgacgtctat cagtcgttcc cggagacgaa gaacacgttc
     5761 cagctcacgt tcccgacggg cggcttcggc gggatggtca ccaagccgtg gggcgaccgg
     5821 aagaaaaaca ccgcccagct ccaggtggaa gcggccgcac aattctcgaa gatcccggga
     5881 atccgggtca tctccctcgt cccacccccg ctccccggtg gcggcgattt ccccgtggat
     5941 ctcgtcatcg cgtcgaccgc ggagccgcag cagctcatcg agttctcgaa ccagctcgtc
     6001 cagaaggcgt acgcgagcgg actcttcatg tttgccgatg cggacttgaa gtatgaccag
     6061 ccccaggccg aggtcgtctt caaccgcgac caggtgcgct cgcaaggagt cgagctgagc
     6121 caggcgggga aggacctctc gacgctactc ggcggcaact acgtcaaccg gttcagcatt
     6181 cagggccgga gctacaaggt gatcccgcag gtgaagcgga cggagcggct cacccccgat
     6241 cagctcgaga acatgtacgt caccgggacc gacggcggac tggtgccgct ctcgaccttc
     6301 gcgtcgatcc agtcgagcac gcagccgcgc gagctgaagc gcttccagca gctcaacgcg
     6361 gtccggatcc agggagtcgt tcctccgaac gtgtcgctgg accaagcgct cacgtatctc
     6421 gagggagaag cgaagaagat ccttccgcag ggctacacga tcgactacgc gggcgagtcg
     6481 cggcagcttc ggacggaagg gagccggttc ctcggcatct tccttctctc ggcggtcctg
     6541 atcttcctgg tgctcgcggc ccagttcgag agcttccggg atccgttcgt gatcctcgcg
     6601 gggtcggcgc cgctcgcggt gagcggggcg ctcctttttt cgttcctggg attcacgagc
     6661 ctcaacatct acagccaggt cgggctcatc acgctggtcg gactggtggc gaagaacggg
     6721 atcctgatcg tgcagttcgc gaaccagctc cgggagacgg gcatcgccaa gctcgaggcg
     6781 gtgatcgaag cgtcgggcac gagacttcgt ccgatcctca tgaccacggc ggccacggtg
     6841 ttcggccacc tgccgctcat cttcgcgagc ggaccggggt cgggcgcgcg aaactcgatc
     6901 gggatcatgc tggtgaccgg catgatcatc gggacggcgt tcacgctctt cgtggtgccg
     6961 tcgatctaca tgctcgtggc gcacgcccag gagcgggtgg cggttcgtga gaaagaggcg
     7021 gcgcccggag acttgggtga ggctccggag gaaatcccag cggtcgcgac cgtctgaggg
     7081 aggagtgacc atggcgacat tcgggagggt caccccacga gcaggccagg tgcggtacca
     7141 tccccagacc tgatgcttcc aacgacacga attctgacgg cgatgctgat cgctgccgcg
     7201 gccctgacga tcccggggcc gccggcggcg acggcggccg acctcaagaa ggtctacgga
     7261 gcggcactcg gaggcgacat ggcgagagcc ctcgccatcc tcgactcgat cgacgtgtcc
     7321 cagctgagcc cgaaggactc caccgcccgg gaatgcctga agcggacctt cgggaacccg
     7381 ccggtgcacg aagacctccc gccgaagtcg ggacagatcc tggatgcgta taggacctac
     7441 tggcagaccg cgatgctcag gcgtgcgccc caggccgacg cggagcgaac cctgctcggc
     7501 actctccgat ccatcctcgc gatcgcaccc acggacaccg cccgcctcga tgacgtcagc
     7561 gagcgcgctc gcctcgcgat cgccggagaa aggctctttg cactgaccgg cgtcacgctg
     7621 ccctactacg agctgatgat ctggcggacc caggagccaa ccacgtacca cgtgaagctt
     7681 cccgagcgaa cgatcgaggt ccatgtcgtg ttcctcgacg acttcgtgtc cctggggtgg
     7741 gctggctatg cgacgtgcgg gcgtgcgcac tccggaggct gggcgacaca ggactcgctc
     7801 tttgctctga agtccgccta cg
//
LOCUS       DASTKC010000002         4599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1184013, whole genome shotgun sequence.
ACCESSION   DASTKC010000002 DASTKC010000000
VERSION     DASTKC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184013"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1057)
                     /locus_tag="VFR25_00035"
     CDS             complement(161..1057)
                     /locus_tag="VFR25_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939464.1"
                     /translation="MDASLYLGPLVQLPIEWLALALLIVACLFWERAGLSGLGVEGCV
                     ASAWLGLFFGYEATGQIAVAVFAALGAALVFAAAAGTLVQLTRVDPALGTFVASLVPW
                     SALVLLLRSHAPLLATQSADAWLLQGTTFDDTYAAVLALNPAVWAAPLLIVLGAAILA
                     NTSFGLRLRAFGENPAWWVPGAPPTAYRIGAAVVGGAFAAPAVVLLARSHAAAPPAAL
                     GILALACVIAARWSLVPAVLLALGPALVRAARPLGGTKPEWGIALDLAPYLIALVYLM
                     LLSRRALRLALSPQTGTDPDVL"
     gene            complement(1066..1707)
                     /gene="udk"
                     /locus_tag="VFR25_00040"
     CDS             complement(1066..1707)
                     /gene="udk"
                     /locus_tag="VFR25_00040"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET9939465.1"
                     /translation="MTPGANRRGILIGIAGGTGAGKTLVAQSISEDIGSEKVLILEQD
                     SYYKDLTNIPLGERESRNFDHPEAFDRDLLRSHLEALLGGGEADIPIYDMRTHTRHPQ
                     PVKAKGRRIIILDGILILDDPAIRQLMDIKLYVDADPDIRFIRRLKRDLTERGRTLDQ
                     VIRQYEASVRPMHLQFVEPSKRYADLVIPEGGYNAVAIDLLKTKVRSLLREAG"
     gene            complement(1704..2252)
                     /gene="hpt"
                     /locus_tag="VFR25_00045"
     CDS             complement(1704..2252)
                     /gene="hpt"
                     /locus_tag="VFR25_00045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET9939466.1"
                     /translation="MEVLIGTADIHRRIGELGKQIARDHPHGTPLLVGVLNGCIFFVA
                     DLMRAIPIPHELDFVSVTSYGSSSESSRAPRLVKDLEREIAGRDVILVEDIVDSGHTL
                     SFLRDTLLARGPRSLKIATLLDKRERREREVPLDYVGFVVPDRFLVGYGLDFAERFRH
                     LPYIAAIQPMELEPDRVPETAR"
     gene            2414..2803
                     /locus_tag="VFR25_00050"
     CDS             2414..2803
                     /locus_tag="VFR25_00050"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HET9939467.1"
                     /translation="MAEQVVMERRNGQRRRSDRRAQGRMEQQMLYLLWGVGILNYTDA
                     AQTVYLLNAKMMIEANRIMAFLLDHSPYVFWMYKTLVPTLGCVLLWRYRRRVRWLYGA
                     VLTVFVVYLTVVIRSFLYMALPIAPHA"
     gene            3055..3858
                     /locus_tag="VFR25_00055"
     CDS             3055..3858
                     /locus_tag="VFR25_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939468.1"
                     /translation="MGGPHYLKLAIQPDLKAPSAHEDVGYFFINSFLLSRIDSDLPSD
                     LAEFDEDVNIYTASLLSGVVSGREDLLRADLVSTRDADVFARVQESKDQRYRYRVYKA
                     NADFLYVSLGIFSEGMAGVDPVRATDEDADRLTGRGKSYYQFASAYAERLYGPKAGIT
                     EVMDKLSRNFEAYTKVMTWMSGEYLNLVRRLSAGEVFHIERSAQESFEGEHKRATWDA
                     LLDSYVEWQKTHAKDARRRLMRAARELKRIDPEFSLRVFREKWGPPRRP"
     gene            3952..>4599
                     /locus_tag="VFR25_00060"
     CDS             3952..>4599
                     /locus_tag="VFR25_00060"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9939469.1"
                     /translation="MNSPLRIGMLGCGVVGTGLLHLLRDRASEIEQAVGAPLRVMRVA
                     VADPKKRREAALEGATVGGDAMAVSEAEDIDLLVEVMGGADRALPPVTRALERGLPVV
                     TANKLLVANHGAELEAIAARTGATLRYEASVAGVIPVLQILDHGLRTERFQLLVGILN
                     GTTNYILSTMEREGRDFAEALEAARKLGFAESDPSLDLSGADTAQKLTILVRRAFP"
BASE COUNT          748 a         1544 c         1607 g          700 t
ORIGIN      
        1 atgctcgctt cccaggccac tccttccgcg agaactccga gcccgcttcc caggatgagc
       61 ccgacgcgcg gtgatgattg cgtgcggcgt cggacgacgc ggagcgcttc gtcgatgtcg
      121 atcgcgaccg cgccggtcgc ggagcgcgga ccagccgcca ttacaggacg tccgggtcgg
      181 tgccggtctg cggcgagagc gcgaggcgga gcgcccgcct cgagaggagc atcaggtaga
      241 cgagcgcgat gagatacggc gcaaggtcga gagcgatgcc ccattccggc tttgtgccgc
      301 cgagagggcg cgccgcgcgc acgagcgcgg gccccagggc gaggagcacc gccggcacga
      361 gcgaccagcg cgcggcgatg acgcacgcga gcgccaggat cccgagcgcc gcgggaggcg
      421 cggcggcgtg cgagcgcgcg agcaggacga ccgccggggc ggcgaacgct ccgcccacga
      481 ccgcggcgcc gatccggtac gcggtcgggg gcgcgccggg aacccaccac gccggattct
      541 ctccgaacgc gcggagcctg agaccgaacg acgtgttcgc gaggatcgcg gcgccgagaa
      601 cgatgaggag cggcgcggcc cagaccgccg gattcagagc gagcacggcc gcgtaggtgt
      661 cgtcgaacgt cgtcccctgg aggagccagg catccgctga ctgcgtggca aggagcggag
      721 cgtgggagcg caggaggagg accagcgcgc tccacgggac gaggctcgcg acgaaggttc
      781 cgagggccgg atcgacgcgc gtgagctgga cgagcgttcc cgcggcggcc gcgaagacga
      841 gcgccgcacc cagggccgcg aacacggcca cggcgatctg tcccgtcgcc tcgtagccga
      901 agaagagtcc cagccacgcg ctcgcgacgc atccctcgac cccgagaccg gagaggcccg
      961 cgcgctccca gaagaggcac gccacgatga ggagcgcgag cgcgagccac tcgatgggga
     1021 gctgcaccag cgggcccagg tagagcgaag cgtccacgcc ggccctcagc ccgcttcgcg
     1081 caacagggag cggaccttcg tcttcaggag atcgatcgcg accgcattgt agccgccctc
     1141 gggaatcacg agatcggcgt agcgcttcga tggctcgacg aactggagat gcatcggccg
     1201 cacgctcgct tcgtactggc ggatcacctg gtcgagcgtc cgtccgcgct cggtcaggtc
     1261 gcgcttcagt ctccggatga accggatgtc gggatccgcg tcgacgtaga gcttgatgtc
     1321 catgagctgg cggatcgccg gatcgtcgag gatcaggatg ccgtccagga tgatgatgcg
     1381 gcgtcccttc gccttcaccg gctgcggatg ccgcgtgtgc gtgcgcatgt cgtagatcgg
     1441 aatgtcggcc tcgccgccgc cgaggagcgc ctcgagatgg ctgcgcagga gatcgcggtc
     1501 gaacgcctcg ggatggtcga agttacggct ctcgcgctct ccgagcggga tgttggtgag
     1561 gtccttgtag taggagtcct gctcgaggat caggaccttc tccgagccga tgtcctcgga
     1621 gatggactgc gcgacgaggg tcttgcccgc tcccgtgccg cccgcgatgc cgatgaggat
     1681 gccgcgccgg ttggcccccg gcgtcaccga gcggtctccg gaacgcgatc gggctcgagc
     1741 tccatcggct ggatggcggc gatgtagggg agatgccgga agcgctcggc aaagtcgaga
     1801 ccgtagccga cgaggaatcg gtcggggacc acgaagccga cgtagtcgag tgggacctcg
     1861 cgctcgcgcc gctcccgctt gtcgagaagg gtcgcgatct tcagtgagcg cggaccgcgc
     1921 gcgaggagcg tgtccctcag gaaggagagc gtgtgcccgc tgtccacgat gtcctcgacc
     1981 aggatgacgt cgcggccggc gatctcgcgc tcgaggtcct tcacgaggcg cggagcgcgg
     2041 ctcgactcgc tcgatgagcc gtaggacgtc acgctcacga aatccagctc gtgcgggatc
     2101 gggatggcgc gcatgagatc ggcgacgaag aagatgcagc cgttcaggac gccgacgaga
     2161 aggggggtgc cgtgggggtg gtcccgggcg atctgcttgc ccagctcccc gatccggcgg
     2221 tggatatcag ccgttccgat cagcacttcc atggcaggcg gctctcccgt tgggcccgcg
     2281 gcactctagc atcggcggaa gcggggtgtc aaaccgccgg ccgggcctgc caattccgcg
     2341 caagttccat cccaccttgc cgataatagg cggagactcg cgcaggacag gtgctcctcg
     2401 aaggaggaag ggcttggccg agcaggtggt catggagcga aggaacgggc agcggaggcg
     2461 gtccgaccgg cgggcccagg ggcgcatgga gcagcagatg ctctacctcc tctggggcgt
     2521 cggcattctc aactacacgg acgccgccca gacggtctac ctgctcaacg cgaagatgat
     2581 gatcgaggcg aaccgcatca tggcgttcct cctcgatcac tcgccctacg tgttctggat
     2641 gtacaagacg ctcgttccca ccctcggctg cgtgctcctc tggcgctacc ggaggagggt
     2701 gcgctggctc tacggcgcgg tgctcaccgt gttcgtcgtc tatctcaccg tcgtgattcg
     2761 aagcttcctc tacatggccc tgccgatcgc gccgcacgcc tgaccgcccg tcgttcccgc
     2821 tgaccgtgca agattccact gggtggcttt tcgcatcacc gtggtggcct gggcgcaatt
     2881 ccccagccgt cgccggcgcg cgggcgacgg ccggaacttt ctggaaaatc gtcggttcga
     2941 aggagtggca ccgccccctg atgggccggc cggcggtgca atatccaatg gcatgacacg
     3001 tgcaattccc cccctccggc gcgtagatgg ttgcgcctgg acagggggtg tcctatgggc
     3061 ggtccacact atctgaagct ggcgatccag cccgacctca aagcgccgtc cgcgcacgag
     3121 gacgtcggct acttcttcat caacagcttc ctcctctcgc gcatcgattc ggaccttccg
     3181 tccgacctcg ccgagttcga cgaggacgtg aacatctaca cggcgtcact tctctccggc
     3241 gtggtctcgg ggcgcgagga tctgctccgc gccgacctcg tctcgacgcg cgacgcggac
     3301 gtgttcgcgc gggtccagga gagcaaggac cagcgctatc gctaccgcgt gtacaaggcc
     3361 aacgcggact tcctctacgt ctcgctcggc atcttcagcg agggcatggc gggcgtggat
     3421 cccgtgcgcg ccaccgacga ggatgcggac cgtctcacgg ggcgcggcaa gtcctactac
     3481 cagttcgcgt ccgcgtacgc ggagcggctc tacgggccca aggcgggcat aaccgaggtg
     3541 atggacaagc tctcgcggaa tttcgaggcc tacaccaagg tgatgacctg gatgagcggc
     3601 gagtacctga atctcgtgcg ccggctgagc gcgggcgagg tcttccacat cgagcgctcc
     3661 gcgcaggagt cgttcgaagg cgagcacaag cgcgcgacct gggacgcgct cctcgacagc
     3721 tacgtggagt ggcagaagac ccacgcgaag gacgcccgcc gccggctcat gcgcgccgcg
     3781 cgcgagctca agcgcatcga cccggagttc tcgctccgcg tcttccgcga gaagtggggc
     3841 ccgccccggc gtccgtagct ccttcccgat ccatgagaac gcctgaagcg gccgcccgag
     3901 cggccgcttc gtttgacacc ccccgcgcgg tccagtagct tggaccggct catgaattct
     3961 cctctgcgaa tcggcatgct cggctgcggc gtggtgggta cgggactcct ccacttgctg
     4021 cgcgaccgcg cctccgagat cgagcaggcg gtgggcgcgc ctctacgtgt gatgcgcgtc
     4081 gccgtggcgg atccgaagaa gcggcgcgaa gccgcgctcg agggtgcgac cgtggggggc
     4141 gacgcgatgg ccgtctcgga ggcggaggac atcgacctcc tcgtcgaggt gatgggaggc
     4201 gccgaccgcg cgctccctcc cgtgacccgc gcgctcgagc gcggcctccc ggtcgtcacc
     4261 gcgaacaagc tcctcgtggc gaatcacggc gccgagctcg aggctattgc cgcgcgcacg
     4321 ggagccacgc ttcgctacga ggcgtccgtc gccggcgtga tcccggtgct ccagatcctg
     4381 gaccacggcc tccgcaccga gcgcttccag ctcctcgtcg ggatcctgaa cggcacgacg
     4441 aactacatcc tctcgacgat ggagcgggag gggcgcgact tcgccgaggc tctcgaagcg
     4501 gcgcggaagc tcggcttcgc ggagtcggac ccaagcctcg atctctccgg cgccgacacc
     4561 gcgcagaagc tcacgatcct cgtgcggcgc gcgttcccc
//
LOCUS       DASTKC010000003         8052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_948414, whole genome shotgun sequence.
ACCESSION   DASTKC010000003 DASTKC010000000
VERSION     DASTKC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948414"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFR25_00065"
     CDS             complement(<1..429)
                     /locus_tag="VFR25_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939470.1"
                     /translation="MDALRIAVLLPHLGVYGGIRRFFELGRVWTARGHEVALLTPPSA
                     GVARPWLPFAGRIGILDDLPRTTWDVLLSPDPVLFLQSKVPGALRAFYAVLEGAPRAR
                     EAWRSADLVIANSLGMARHVARNRVRAVHAPGGVNTEFFRP"
     gene            531..1565
                     /locus_tag="VFR25_00070"
     CDS             531..1565
                     /locus_tag="VFR25_00070"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9939471.1"
                     /translation="MSALPGVPGHEAPAAEAIAKAFSIHADRVERDRAGNVRGWIEGS
                     APKPRPRALFAAHMDRIGFLVKAIEPGGFIRLTELGGFDSRTLLAKEVVIHSSPPIVG
                     LFATKPPHLLKKGEADRVIPSEDLYIDTGRSERDVRRMVPIGTPVTLRQPPVDLLGGR
                     LAAPAMDDRAGVAVLLGMLEILHTNRPEWDVVMVATVEEEFGLYCLGAKTATESIGPQ
                     LGVAIDVTHGDMPGVKEGDTYPLGSGPSICVGGNIHPLVFEGMKRSARTLGLPYSVEG
                     CPITSATDAMDIQIAREGVPTGVIGIPCRYMHTGIETVAPEDVDRCARVLAHFVASLE
                     PSWRAEEVLK"
     gene            1562..2641
                     /locus_tag="VFR25_00075"
     CDS             1562..2641
                     /locus_tag="VFR25_00075"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9939472.1"
                     /translation="MIAPPFDDALLGRLSEASGVSGGEDAVRRILIDDVRDHVDTLEI
                     DALGSLVARISPAAPRSSRPKSSRRPRNGPHVLLCAHMDEVGLMVFAIEKDGRLRVRR
                     VGGIDQRILVGQVFRVGANGLKGVLGALPPHLVPRPEREKVISIDDMYLDIGASTREE
                     AERHVAVGDYACFDTTYEKWGSTRKGKAFDDRVGCTVLASLVKKRPPVPVIAVWSVQE
                     EVGLRGAGAAARRANADVAIVLEGTASGEAPGASPGESAPVMGGGPALTVQDRTLMAD
                     ARLVDLLERTGKERKIPTQWKRPGVGGTDAGRISLSGAGIPSAVVSVACRYIHAPAAY
                     LDVRDPQRVTALVWHTLESLRKEWT"
     gene            2638..3651
                     /locus_tag="VFR25_00080"
     CDS             2638..3651
                     /locus_tag="VFR25_00080"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939473.1"
                     /translation="MSSLESNVDALLPELLAAFGPSGRESGVRRTLRKALRGLGTLSE
                     DATGNLRLHRPGRGPRLLLATPMDAAGVIVTRVDASGLGRVTLLGARAPVELVGSTVV
                     FEDGRKALLGYDRGPGSKNGVDLEADQLFLETGLGKKGEREFPVGSVGAIEDRPARLG
                     EFWCGANLEGRAGCAAVVAALRVARSSRYDLHVVFAAQSDLGSRGATTGAFGIDPELA
                     IVVDVAHLEGKETPGIAVGKGPCLGLKESGYVAHPEALALLKKAAAAARLKTQWLIRE
                     HEGTDARAIRAARTGVPTALVAIPARRSGAAFSLAHARDITQTAALVAKLLAGAPAKG
                     GSR"
     gene            3648..4847
                     /locus_tag="VFR25_00085"
     CDS             3648..4847
                     /locus_tag="VFR25_00085"
                     /inference="COORDINATES: protein motif:HMM:NF005744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9939474.1"
                     /translation="MKVSARGVEAQASPIRRLAPHADEAARSGAKVYYLNIGQPDLPT
                     PDIVMDRIRSYPGKYVPYSPSAGTAEFQKGMAGYYQQHGLSVAPAEVLTTVGGSEALQ
                     FAFWALFDPGDEVLLFEPFYTNYATMALLAGVSVKAMTCDGRNGYHLPPIEAVERAVG
                     PKTRGILICAPSNPTGTAYTAAEIDAIVDLAKRKDLWIITDEAYREFIYDGGKCESPM
                     TRPGADQRTVLVDSISKRLNMCGARVGALVTKNAAVREACLKFAQARLSPPTLGQYAA
                     SVIDQVPAAYTQGVVAEFKKRRDLVLDGLAKIPGTFARKPEGAFYLMAELPVPDAEEF
                     VIWMLREYRKDNATIMVAPGDGFYATPGRGKNEIRFAYVLETGALKAAMEVLAGALEA
                     YPVKAAR"
     gene            4856..5350
                     /locus_tag="VFR25_00090"
     CDS             4856..5350
                     /locus_tag="VFR25_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939475.1"
                     /translation="MRLAVIGLFLAAQASAQPARDSWPVGAAPESRVRVTAPRSSVPQ
                     AVGRVHHWTPDSLVIQLDPSQAAAPAMASFHRTSITRLETSEGLKGHTVRGAFLGFLV
                     GSVAGFFVAEAVLDEDADPYGTSFDGGAVVAVWAGTTVLTTVLGGIIGGAEKSERWKD
                     VGGF"
     gene            5628..>8052
                     /locus_tag="VFR25_00095"
     CDS             5628..>8052
                     /locus_tag="VFR25_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9939476.1"
                     /translation="MISRAWMTVALSVLTALLLVSPAHAQYMYLDSNGDGVRSTADVV
                     NSAGPTTIDVWLDTAHNRDGTLAVCYPDTTQPNTIGSYVFSLRAANGTAIWSGFVNRQ
                     STMTVAFGEHSSSTEYENGYGGPSLLSAGLYRLATIVLTIQSGTPSVDIVPSITGAVD
                     VTSFGSACYGHDFDNTMKLGTDWYDVDGLPFGTGGSNQPPLLAQPDDMTVASGTLAFQ
                     TLTATDADHQTLTFSKTVGPTFLIVTTLDPGAGTATGQIALSPTTGDVGTSTGTVAVT
                     DGFASAERSFQITVTEGANRAPSLSAPAIVTAVAGTTPRFPLSASDPDGQTLSFHKIA
                     GPDYAHVATLASGMGGAVGSLDLDPTACDAGVTAATVEATDGIASDSKSIDILVKTRG
                     GAPAQPPAVAAYGALVLAAGDLNGDQHVDLLAGGQDGELTTLIGHGDGSFTTSAQGVR
                     GSELTSVTLGDWNSDGMLDAAITVLNPDHIVILSGDGAGGLSQTEFYPASSIPQAIRS
                     GDLDRDGDLDLVVAQSAGVSVYLGNGDGTFATRTDYPMGGVSHGLVLEDFNLDGRLDV
                     ATANLASHDLGIRLGFGNGTLGDAAHIALSAAPFDLIARDWNRDGKLDLAIGMGSDNV
                     RIYTGDGAGRFTAGATLSGFGDLIQLASADLDLDGNDDLIATSLAIVVNQPTIQLAQG
                     HGDGTFGTNRVLTTQEAYGPASADLDEDGFPDLAVAAGNIFLWLNDAGGAGAPEARAF
                     TDSNAPAGGKPTTCMRLEPVAGSYENADLDLTSITLSPVDGGGSIHAVATKSAIVMDT
                     DGNGVAEVPV"
BASE COUNT         1290 a         2754 c         2747 g         1261 t
ORIGIN      
        1 cgggcggaag aactccgtgt tcacgccccc cggggcgtga accgcgcgga cgcggttgcg
       61 cgcaacatgg cgcgccatgc cgagggagtt cgcgatcacg aggtccgcgc tccgccatgc
      121 ctcgcgcgcc cgcggcgccc cttcgagcac cgcgtagaac gcccggagcg cgccgggcac
      181 cttcgattgt aggaagagga ccggatcggg ggaaagcagg acgtcccagg tggtacgggg
      241 caggtcgtcg agaattccga tccgtcccgc gaacggaagc cacggcctgg ctacccccgc
      301 ggacggcggg gtgaggagcg cgacctcgtg tccgcgcgcg gtccagacgc ggcccagctc
      361 gaagaatcgg cggataccgc cgtacacgcc gagatgaggg aggaggacgg cgatccggag
      421 ggcgtccatc ggggcgcgga gtttacattt gcctgccctc ggagggtgga ctaaattcgc
      481 aggctatgcc ccgtccggaa ccacgctcca ccgtcgcttg gctccgattc ttgagcgccc
      541 tccccggggt ccccggccac gaggcccccg ccgcggaggc catcgccaag gcgttctcga
      601 tccatgccga ccgcgtggag cgggatcgag ccggcaacgt gcgcggctgg atcgagggaa
      661 gcgcgccgaa gccgaggccg cgcgcgctct ttgccgccca catggaccgg atcggcttcc
      721 tcgtgaaggc gatcgagcca gggggcttca tccgcctgac cgagctcggc ggcttcgatt
      781 cgaggaccct cctcgccaag gaggtcgtga tccactcgag cccgccgatc gtcggactct
      841 tcgcgaccaa gccgccccat ctcttgaaga agggtgaggc ggaccgggtc atcccgagcg
      901 aggatctcta catcgacacc ggccgctccg agcgcgatgt gcgccggatg gttccgatcg
      961 gaacgcccgt gaccttgcgc cagcctccgg tcgacctcct cggcggccgg ctcgcggctc
     1021 ccgccatgga cgatcgggcg ggcgtggccg tgctcctcgg catgctcgag atcctccaca
     1081 ccaaccggcc cgagtgggat gtggtcatgg tggccacggt cgaggaggag ttcggtctct
     1141 actgtctggg cgccaagacg gcgaccgagt ccatcggccc gcagctcggg gtggcgatcg
     1201 acgtcacgca cggcgacatg cccggagtca aagagggcga cacctacccg ctgggcagcg
     1261 gcccttcgat ctgcgtgggc gggaacattc atccgctcgt gttcgaggga atgaagcgga
     1321 gcgcccgcac gctcggcctt ccctacagcg tcgagggatg ccccatcaca tccgccacgg
     1381 acgcgatgga catccagatc gcgcgcgagg gcgttcccac cggcgtgatc gggatcccgt
     1441 gccgctacat gcacacgggg atcgaaaccg tcgcacccga ggatgtggac cgctgcgcca
     1501 gggtgctcgc acacttcgtc gccagcctcg agccctcgtg gcgcgcggag gaggtgctca
     1561 agtgatcgcc cctccgttcg acgatgcgct cctcggacgg ctctcggagg catccggcgt
     1621 gagcggcggc gaggacgccg tgcgccgcat cctgatcgac gacgtgcgcg accacgtgga
     1681 cacgctcgag atcgacgctc tggggagtct cgtcgcgagg atctcgcccg cggcgccgcg
     1741 atcgtcgcgg ccgaagagct cgcgccgtcc gcgaaacgga ccgcatgtgc tcctctgcgc
     1801 acacatggac gaagtcggac tgatggtgtt cgcgatcgag aaggatggac gcctgcgggt
     1861 ccgccgcgtg ggaggcatcg accagcgcat cctcgtcggg caggtgttcc gcgtcggcgc
     1921 caacgggctc aagggcgtgc tcggcgcgct tccgccgcat ctcgttcccc gccccgagcg
     1981 cgagaaggtg atctcgatcg acgacatgta cctcgacatc ggcgcctcga cgcgagagga
     2041 ggcggagcgc cacgtggccg tgggcgacta cgcttgcttc gacaccacct atgagaagtg
     2101 gggatccacg cgaaagggca aggcgttcga cgatcgcgtg ggatgcaccg tgctcgcgag
     2161 cctcgtgaag aagcgtcccc ccgtgcccgt gatcgcggtc tggagcgtgc aggaagaggt
     2221 gggcctgcgc ggcgcgggag ccgcggcgcg ccgcgcgaac gccgatgtcg cgatcgtgct
     2281 cgagggcacc gcgtccggcg aagcgcccgg cgcatctccc ggtgaaagcg ctcccgtcat
     2341 gggtggcggc cccgctctga cggtgcagga ccgcacgctc atggccgatg cgcggctcgt
     2401 ggatctgctc gaacgaaccg gcaaggagcg caagatcccc acccagtgga agcgccccgg
     2461 cgtcggcggc accgacgcgg gacgcatctc gctctccggc gcgggcattc cgtccgcggt
     2521 cgtgtcggtc gcgtgccgct acatccacgc tcctgccgca tacctcgacg tgcgcgaccc
     2581 gcagcgcgtc acggcgctcg tctggcacac gctggaatcc ctgagaaagg agtggacgtg
     2641 agctcgctcg aatccaatgt cgacgccctg cttcccgagc tcctcgccgc cttcggtccg
     2701 tcgggtcgcg agagcggcgt tcgccgcacg cttcggaagg cgctgcgcgg tctcggcacc
     2761 ctgagcgagg acgcgaccgg gaacttgagg ctccaccgcc cgggccgcgg accgcgtctt
     2821 ctgctcgcca ctccgatgga cgcggcgggt gtcatcgtga cacgcgtgga tgcgagcggg
     2881 ctcggtcgcg tcacgcttct cggcgcccgc gcgccggtcg agctcgtggg ctccaccgtg
     2941 gtgttcgagg acggccggaa ggcgctcctc gggtacgacc gcggaccggg atcgaagaac
     3001 ggcgtcgacc tcgaggcgga tcagctcttc ctcgagaccg gcctcgggaa gaagggcgag
     3061 cgcgagttcc ctgtcggatc cgtcggcgcg atcgaggatc gtcccgccag gctcggcgag
     3121 ttctggtgcg gggcgaatct cgagggacgc gccggctgcg ccgcggtcgt ggctgcgctt
     3181 cgggtcgcac gctcgtcgcg gtatgatctc cacgtcgtct tcgcggcaca gtccgacctc
     3241 ggctcccgcg gcgccacgac cggcgcgttc gggatcgatc ccgagcttgc gatcgtggtc
     3301 gacgtcgcgc acctggaggg gaaagagaca ccgggcatcg ccgtcggcaa ggggccgtgt
     3361 ctcgggttga aggaatcggg ctacgtcgcg catccggagg ctctcgcctt gctgaagaag
     3421 gccgccgcgg cagcgcgttt gaaaacacag tggttgatcc gcgagcacga agggaccgac
     3481 gcgcgcgcga ttcgcgccgc gcggaccggg gtcccgaccg cgctcgtggc cattcccgca
     3541 cgtaggagcg gcgccgcgtt ctcgctcgcc cacgcgcgcg acatcacaca aaccgcggcg
     3601 ctcgtcgcga agctactcgc gggcgcgccg gccaagggag gatctcgatg aaggtctccg
     3661 cacgcggggt cgaggcgcag gcctccccca tccgccggct cgctccgcac gccgacgagg
     3721 cggcccggag cggcgccaag gtctactact tgaatatcgg acagcccgac ctcccgacgc
     3781 cggacatcgt gatggatcgg atccgctcgt accccggcaa gtacgtcccg tacagcccgt
     3841 ccgccgggac cgccgagttc cagaaaggca tggccggcta ctaccagcag cacggactgt
     3901 cggtcgcgcc cgcggaagtg ctcacgaccg tcggcgggag cgaagcgctc cagttcgcct
     3961 tctgggcttt gttcgatccg ggcgacgagg tgttgctgtt cgagccgttc tacacgaact
     4021 acgcgacgat ggcgctgctg gcgggcgtgt cggtgaaggc catgacctgc gatggacgga
     4081 atggctacca cctgcccccg atcgaagccg tggagcgcgc cgtgggaccg aagacgcgcg
     4141 ggatcttgat ctgcgcgccg tccaatccca ccggcacggc gtacacggcg gccgaaatcg
     4201 acgcgatcgt ggatctcgcg aagcgaaagg acctctggat catcaccgac gaggcctatc
     4261 gcgagttcat ctacgacggc ggcaaatgtg agagcccgat gacgcgcccg ggcgcggatc
     4321 agcgcacagt gttggtggac tcgatctcga agcgcttgaa catgtgcggc gcccgcgtgg
     4381 gcgccctggt gacgaagaac gcggccgtgc gcgaggcgtg cttgaagttc gcgcaggcgc
     4441 ggctgtcgcc gccgaccctg ggccagtacg cggcctcggt gatcgaccag gtacccgcgg
     4501 cgtacacaca gggggtcgtc gccgagttca agaagcggcg cgatctcgtg ctcgatgggc
     4561 tcgccaagat ccccggcacg ttcgcgcgga agccggaggg tgcgttctat ctgatggccg
     4621 agctccccgt gcccgatgcg gaggagttcg tgatctggat gctccgcgag taccggaagg
     4681 acaacgcgac gatcatggtc gcgccgggcg acggcttcta cgcgacgccg ggccgcggca
     4741 agaacgagat ccggttcgcc tacgtgctcg agaccggtgc tttgaaggcc gcgatggagg
     4801 tcctggccgg agcgctcgag gcgtatccgg tgaaggcggc gcggtaggcg ccgcgttgcg
     4861 cctcgcggtc atcggtctct tcctcgccgc tcaggcgtcc gctcagcccg cccgcgattc
     4921 ctggccggtt ggcgcggccc cggagtcacg ggtccgggtc actgctccca ggtcatcggt
     4981 cccacaagcg gtgggccggg tccatcactg gacgcccgac tcgctcgtga ttcagctcga
     5041 tccctcccag gccgccgcgc cggccatggc ttcgtttcac cggacctcga tcacccgcct
     5101 cgaaaccagt gaaggcttga aggggcacac cgtacgcggc gccttcctcg gcttcctggt
     5161 cggcagcgtc gccggattct tcgtggccga ggctgtcctg gacgaggacg ccgatcccta
     5221 cgggaccagc ttcgatggcg gagccgtggt cgcggtgtgg gccggtacta ccgtgctcac
     5281 aacggtgctt ggggggatca tcggaggggc tgagaagtcg gagcggtgga aggacgtcgg
     5341 cgggttctag cgtatggccg gtgccggagg gaggcgtgga cgtgtgactt agacaccttg
     5401 gcgcttttct tcgcaatgcg ggtgcgcttt tctgggggac gcgcgttttc tcaggtgagt
     5461 ggcccgcgtc ctcgggccat ggacaccgcc ttgggtatca ttggccgcgg cggcgcagga
     5521 gttgtagtct cgactcccct ggtgcaagag tgcccatgat cgcacccttc cccccaggtc
     5581 cccacgccag catcgccgaa cgtaggatcg aaaagggggg ctccgccgtg atctcgcgtg
     5641 cctggatgac cgtcgctctc tccgttctga cagccctgct ccttgtaagc cccgcccacg
     5701 ctcagtacat gtacctcgac tccaacgggg acggcgttcg ctcgaccgcc gatgtcgtga
     5761 actccgcagg accgacgacc atcgacgtct ggctggatac ggcgcacaac cgagatggaa
     5821 ccctggcggt ctgctatccc gacacgaccc aacccaacac gatcggctcg tacgtattct
     5881 cgctgcgggc cgctaacggc acggcgatct ggagcggctt cgtcaatcgc cagtccacga
     5941 tgaccgtcgc cttcggagag cactcgagct cgaccgaata cgaaaatggc tacggggggc
     6001 ccagtctctt gtccgcgggg ctctatcgcc tggctacgat cgtcctgacg attcaatccg
     6061 gaacgccatc cgtcgacatc gtgccttcca tcacgggcgc cgtggacgtg acgagcttcg
     6121 gctcggcctg ttacgggcac gacttcgaca acacaatgaa gctgggcacc gactggtacg
     6181 acgtcgacgg cctcccgttc ggaacgggcg gctccaacca gcctccgctc ctcgcgcagc
     6241 cggacgacat gacggtggcc agcgggacgt tggcgttcca gaccctcacg gcgaccgacg
     6301 cggatcatca gacgctcacc ttctccaaaa cggtcggccc gaccttcctg atcgtcacga
     6361 ccctggaccc gggagcggga acggccacgg gtcagatcgc gctctccccc acgaccggcg
     6421 atgtcggcac gagcaccgga acggtcgctg tgaccgacgg tttcgcgtcg gccgagcgtt
     6481 cgttccagat caccgtgacc gaaggagcca atcgcgctcc gtcgctgtcg gcccctgcca
     6541 tcgtgacggc ggtcgccgga accacgccgc gcttcccgct cagcgcgtcc gaccccgatg
     6601 ggcagacgct ctcgttccac aagatcgccg gccccgatta cgcgcacgtc gcgacgctcg
     6661 cgtcgggaat ggggggcgcc gtgggaagcc tcgacctgga tccgactgcc tgcgatgccg
     6721 gcgtgaccgc agccaccgtc gaagccacgg atggcatcgc gagcgactcc aagtccatcg
     6781 acatcctcgt gaagactcgc gggggtgcgc cggcgcagcc tccagccgtc gcggcctatg
     6841 gcgcgttggt cctggccgct ggagacttga acggcgatca gcacgtcgac ctcctggcgg
     6901 gcgggcaaga tggcgagctc acgaccctga tcggtcacgg ggacggctca ttcacaacaa
     6961 gcgcccaggg cgtgcgggga tccgagctca cgtccgtgac gctgggggac tggaactcgg
     7021 acggcatgct ggacgccgca atcaccgtcc tgaatccgga ccacatcgtg atcctgagcg
     7081 gcgacggggc gggcgggctg agccagacag agttctatcc cgcatcgagc attccccaag
     7141 ccatccggtc cggcgacctg gaccgcgacg gcgacctcga cctggtcgta gcccagagtg
     7201 ccggagtgtc cgtgtacctg gggaacggcg acggcacgtt cgcgacccgg acggactatc
     7261 ccatgggggg cgtgtcccac gggctcgtcc tcgaggactt caatctggac gggcgtctcg
     7321 atgtggcgac cgccaacctc gcgtcccacg atctcggcat ccgcctcggc ttcgggaacg
     7381 gcacgctcgg agatgccgcg cacatcgccc tcagcgcagc tccattcgat ctgatcgcgc
     7441 gcgactggaa tcgcgacggc aagttggatc tcgccatcgg catggggagc gacaacgtac
     7501 gcatctacac cggtgacgga gccggccggt tcacggcggg tgcgacgctc tccgggtttg
     7561 gggatctcat ccagcttgcg tcagcggacc tggacctcga cgggaacgac gacctcatcg
     7621 cgacgagctt ggccatcgtg gtcaatcagc cgaccatcca actcgcgcag ggccatgggg
     7681 acggaacatt cggcacgaat cgagtgctca ccacccagga ggcctacggc ccggcaagcg
     7741 ccgatctcga cgaggatggc ttccctgacc tcgcggtggc cgccggtaac atattcctct
     7801 ggctcaacga cgcgggcgga gccggcgcac cggaagcccg cgcctttaca gattccaacg
     7861 ctcccgccgg gggcaaaccg accacgtgca tgcgcctcga gccggtcgcc ggatcgtacg
     7921 agaacgcgga cctggacctc acgtcgatca cgctgtctcc ggtggacggc ggcggcagca
     7981 tccatgctgt cgccaccaag tccgcgatcg tgatggatac ggatggcaac ggagtcgccg
     8041 aagtgcccgt tt
//
LOCUS       DASTKC010000004        22143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_475798, whole genome shotgun sequence.
ACCESSION   DASTKC010000004 DASTKC010000000
VERSION     DASTKC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 22143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22143
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475798"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..287
                     /locus_tag="VFR25_00100"
     CDS             30..287
                     /locus_tag="VFR25_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HET9939477.1"
                     /translation="MKIERSFTRVAARRLVRALSLAGLAALTAGCAATSVKPWDRDLL
                     AQKKMQLVPHPMVNAIDEHIYFSKEGSTGGLDVGGGGCGCN"
     gene            278..1600
                     /locus_tag="VFR25_00105"
     CDS             278..1600
                     /locus_tag="VFR25_00105"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HET9939478.1"
                     /translation="MQLASPGQASIRGKLGTAACMLLASVAPAAARGDASGAVTQVDA
                     TMLLYGEQSRTNVIEPTVRVTRIQPSGRSLSGQIVLDAMTGATPTGAMPPGVVDSTAT
                     LPPGEEARRVQTMTAASGGGGGGSEHAPDPGAIPLAQFNDTRVALDAEWHEPITSAFG
                     STLGGYYSREKDYESIGGSARISLDFLHKLTTLTIGGSANQDHVFPSGGTPLPLSDGS
                     VLHEERNAKHVTGGLVGLSRVITRRWLISVNGSRTYEDGYLTEPYKVVSLIDPATGFT
                     AGQITEGRPSTRERSDVMTTMAYHFRSDVLHLSHRYYWDDWGVRSNTFDLKLRHDTGD
                     SSFLQPHLRYYQQSQADFFTFGLVQGAPIPQYASSDYRLGPLQTATAGLTYGFHLPES
                     PGEFTIRAEYILQFGKGHPDNAVGVQQDFDLMPPVNIGSLVAGYSVAF"
     gene            1699..2529
                     /locus_tag="VFR25_00110"
     CDS             1699..2529
                     /locus_tag="VFR25_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019675038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET9939479.1"
                     /translation="MASPCEVLVDTDDLDEAAHALRIAETEAQRIEAKFSRYRPDNVV
                     HRINHAGGVPVRVDAETAQLLDFAASCHEMSGGLFDITSGSLRRAWTFDGGTRVPEAS
                     QLRALLEHVGWSKVRWDGTAIAMPAGMEIDLGGFGKEYAVDRAAGLVRDEVSRAILVN
                     FGGDLVASGARRDGRPWSVGIEDPERPGAAALYRIEVDRGGIATSGDARRFVLWRGKR
                     LGHILDPRTGWPVEGGPRSVTVLAATCLEAGTLATLAQLQGAGARAFLDAQGARYWTV
                     "
     gene            complement(2550..3710)
                     /locus_tag="VFR25_00115"
     CDS             complement(2550..3710)
                     /locus_tag="VFR25_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9939480.1"
                     /translation="MIPPREPVQHPPMRFETRAVHAGGDPDPATGAIAPPLHLSTTFE
                     HGPAGEPRPAGHIYIRESNPTQSRLEEALVAVEGGEVALAFGSGVGAGAACLQALEPG
                     AHILFHGDLYYAFRTMALEYVPRWGMEASFVDMTDLDALRSALRPNTQLVWTESPTNP
                     TMQILDLRAIAALAKGAGALLLVDGTFATPALQRPLELGADIVLHSTTKYLGGHSDVH
                     GGALVFRTKGELSHRAQHNRHILGAVASPFASWMVLRGLRTLACRMERQSANALAVAR
                     ALEGNRGIRSIHYPGLESDPGHAVAKGQMSAFGAMLSLRIAGGREGAVRACSRVKLFR
                     NATSLGGVESLLEHRRSVESPDSPTPDDLIRVSIGLEHPDDLIEDLRQAVAV"
     gene            complement(3707..4606)
                     /locus_tag="VFR25_00120"
     CDS             complement(3707..4606)
                     /locus_tag="VFR25_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9939481.1"
                     /translation="MTALYTLFALVGFAANSILCRLALQPGAIDPASFTVARLASGAI
                     ALALIVRARRRKSRGASHAGSWGSALLLFVYALPFSLAYVSLGVATGALILFFCVQAT
                     MLVGAVVSGERIHWLEVNGIAVALAGLAHLMAPGLSAPSPEGAALMALAGVAWGLYSL
                     RGRRSTDAIGDTASNFARSAPMALAAFAVAAPLGHLHLSTHGLLLATASGVLASGAGY
                     AAWFAALPRLNALQAAALQLTVPLIAAVGGIALLGEQATRRLVLSAIFILGGVGLAIG
                     GRALRAREAQRRAARSAPERETS"
     gene            4712..8452
                     /locus_tag="VFR25_00125"
     CDS             4712..8452
                     /locus_tag="VFR25_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9939482.1"
                     /translation="MTQDDEHDLGHLVVVGSSAGGIEALGVLVSGLTPDFPAPVVLAQ
                     HLDPSRPSMLPGILERRTKVPVETLTENTKLQRGHVYVVPSNRHVVIQDGTVGLEQDN
                     DDRPLPSIDLLFSTAARVYRERLIAVILTGSGSDGADGAIEVKERGGCVVIQDPRTAA
                     HPSMPSSLPPTLVDHVADLDKIAPLLQDILKGTTIERRIETAHSSDLDEILGILTRQG
                     SIDFRQYKSTTILRRLSRRMALNHIHGLEEYRDFLALNPQEVAMLVRSLLIKVTEFFR
                     DADAFAVLEREIMPELIERGRGRGRVLRLWSAGCATGEEAYSLGLMVSHMLGRELPEW
                     SIKIFATDADGDAIAYARRGVYPATVLRSLPAEYKARYFEPLDQGLRISKALRQMIIF
                     GQQDLAQGVPFPRIDLVSCRNLLIYFKPELQQAVLDLFAYSLQQTHGFLFLGKAETAR
                     PSRSTYEIVNKKWKVYRCLSGPLPIAVRPRGEKGRGESPAVSGPATLNAETLQAAGAR
                     IPEEVELRRVNELFLRSLSVGVCAIDRSYKILSVNAMARRLFGIREVATDQDFLHTVQ
                     GLPYVEIRNAIDRCFRERTVTVLPQVELTDSLGEARFLAIQLIPFEGLGVETALICVE
                     NVTELVQTQRRLEAVQSEQSQLAQELGTANHRLMDMNRDLQDANEELQATNEEMMLTQ
                     EELQATNEEFEATNEELQATNEELETNNEELQATNEELETTNEELHARTSELQELTRA
                     LSNERSRLTQIVEQAPFHVLLLRGPSLIVEDINPPLIDLFPGTKSGQTSFEDVTTDPA
                     LLPVRAGVRNAFVNGRPWQSGWIRVGQPMDLRFYQFTAVPTHDSGGKVDGIVLYAEDV
                     TARRIHEEAEKLSRLRLMLEHASQLAMGLFNAEGKLVHASQQFLGMLQRLRKIDPAHA
                     IGMDWERLWFGAPDLLDTFQTAVSEGRAQRLNEVQIEGDPQRAVWDCSLIPITPEGGG
                     AVDFVVVTAVEVTRPVLAREAVEQVDRLRDNFLSLASHELRTPLTPLAAYIEVLAHLL
                     GEKSRTPDWERQVQDVIGKFRRQIGYMSRLTEDLVDISRIRSGKLTLDRRPVDLRHAV
                     EEGRDQALALGANARVQVTSADADLTVHGDEMRLAQVVYNLLSNAMKHASASERIEVR
                     LSEKRDNGRAWGRVEVCDEGPGIPEEYRDDLFLHFVNPTRREGRAARSGLGLGLFISA
                     RIVEQHEGRIGVEDLEHGTCIWFEIPLIDARVGASTPPAPRN"
     gene            complement(8471..8866)
                     /locus_tag="VFR25_00130"
     CDS             complement(8471..8866)
                     /locus_tag="VFR25_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9939483.1"
                     /translation="MRPLQLLLIEDDVDSGEAMSLLLRSEGIHVDWARTGREAIDIFR
                     AVNGGPAIDVILLDLMLPDMDGSVLIEHLRRIRPTPSIVIHSAAGQAEVFDRGRQLGA
                     AAVLRKPTDWTELRGILFACRPSEVAREH"
     gene            complement(8973..10043)
                     /locus_tag="VFR25_00135"
     CDS             complement(8973..10043)
                     /locus_tag="VFR25_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939484.1"
                     /translation="MRQRIVLALVLAAVLSVRCSGDDNPTGPSLEPRSYRMGFSGIPP
                     RNDFNLVIQTINLWSLRADAALVLSEPPWAALLSGIPADSAVRATQLGLVNYYRAKGL
                     QIIVSVDPTNGLNRSAESQPLVDAGRSLAEPAVQQLYRAYVTAMDTILHPDYIGVASE
                     TNLVRAIAPASVYNAVVQAANGAAADVRAVDPNVQLFSTVQVETAWGKLLPPGTFVGV
                     AQDRTDFPFMQVLGLSSYPYLGGFTDPDSIPLDYYTALTSGNPIPVMVIEGGWPSDSM
                     GVAGTPEKQARYIARHAAILDRAEAGAWFQITFTDLDVDSFPVPPGTSLGPFAHLGLV
                     DEHLNPKPALSQWDKVFSRPRR"
     gene            complement(10099..11115)
                     /locus_tag="VFR25_00140"
     CDS             complement(10099..11115)
                     /locus_tag="VFR25_00140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9939485.1"
                     /translation="MAPAHLVDGLPIVLGFLPVLLFLTGLVLLDSYKLVTGRAIAVTM
                     GLGAVAAIVSFAINRSLLGAVLDEGTLRSYAAPLIEEIAKAAYLIYLVRSQRVGFMVD
                     AGIRGFAVGTGFAVVENLYYARALGDYGVLLWLVRGLGTAIMHGSTSAIVGILSKSFA
                     DRHRSSAWWVFLPGLGIAIASHAIYNQFFVNPLLATAIMCLLMPPLVIAVFERSERVT
                     QHWLSSEFDSDLELLDSILTGELGETRVGRYLESIRHRFPGTVVADMLCLLRIEAELG
                     LRAKARLVARSAGVEIPLDDVEADLAEMEFLKKAIGPTGLMAMSPVLRQGGRDEWQLS
                     TLER"
     gene            complement(11147..14137)
                     /locus_tag="VFR25_00145"
     CDS             complement(11147..14137)
                     /locus_tag="VFR25_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939486.1"
                     /translation="MRTRRAAAARRASLLLGLLACLALAPRPARAELKSIETDHLRLI
                     YLSPTLDFIAPYTARCFENSLRFHSKLWNYTPSEKVNVILEDLADYGNAGVWVNPRNS
                     MVVDIAPLNFVYETGPANERINHTMNHEVVHVLALDKPSGRDRFFRSLFRGKVREANE
                     HPETMLYGYLTTPRRAAPRWYHEGIAVFMETWMAGGLGRAQGPYDEMVFRSKVKDGST
                     IYDPLGLEAEGTKVDFQAGVNSYLYGTRFLSYLAYEYEPEKLVQWVGRGSGSKAYYAS
                     QFHKVYGLPLGTAWRNWIDWEKGEQTAQLDSIDLHPITPYRDLSPRALGSISRAYYDP
                     DRREIYAAVLYPGAVAHIAAIPTDGRPLRRICEVKGPALYFVTSLAYDPADKLLYYTA
                     DNSKWRDLCVADPRTGKSRVLIRDARLGDMVLNPADRTMWAVRHFNGVSTLVRLQPPY
                     TNWNRVYSWPYGRDIYDIDISPDGAKLAASCTEIDGTQSLKIFRTEALLAQDTTSTTL
                     YDFGSAVPAGFVFSKDGRYLVGSSYYTGVSNIFRWNFAADSMEVVTNSETGVFRPLPI
                     DGDSLIAFRYTGEGFVPTMIDPKPLADVSAISFLGTQLAEKYPVLTTWKAGSPATVNL
                     DSITTYSGPYRSVSLIRPSSLYPIVEGYKDYTAVGMRLELSDPIQTNTLGLTASYSPS
                     EGLPANERWHVLAGYKRYNLQLSYQHNLANFYDLVGPTKTSRKGEGVVASYTRSLLYD
                     APRSLTFDASAAGYVDLERLPDAQNIDVSPGFRSLVTTDLGLRYENTRASLGAVDREK
                     GYKWSLGMPWNLVRYDRPEGTVWQGFPQGLATLDVGTPMLLPHSSIWLRTAGGFAPGD
                     REEPFANFYFGGFGNNYIDRKEPKRYRDVESFPGFELNEIAGTNFAKALVDWNLAPLR
                     FRRLGTSALYAPWLRTSLFAGGLATNMDLPSLRRTVFDVGIQSDLQIQLLTQYKLMLS
                     GGYARGFEDGERTSDEWMISLKVL"
     gene            complement(14258..15550)
                     /locus_tag="VFR25_00150"
     CDS             complement(14258..15550)
                     /locus_tag="VFR25_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939487.1"
                     /translation="MSTKRIVIGLGVVIAIALGIASFTGVIPPKNGVMGTIGAAKRYQ
                     TTQISAGDVTLTDADMQDLLQSDVFHQLVTDANFREVMRTQSESFRTVLLDKQFRDLM
                     GKQGFAEVMRSDLFKTAVEQNRYDLLAKASPADFQRTQLTERTQSTLDRANAEQLRTQ
                     VTNARTEVAKAQAAYDKSVSELLKTSSTANKQEVAKVEQAKAQDVMARTELAKAQAAY
                     DKTVTELSKMEQAKTEAAKAEVAKADLAKATTDIGKAGWNRTDLAKVEQAKAELAKAQ
                     DALDKTSTMYQKSVTELSKLSAAKTEVAKTEQARTSAEAARTMYMKANQAYERTVNTA
                     LDKAATTEAGRTVQAEAGRTADANAYAQAQELARSEQFKTVFADANFREVAKSNEFAK
                     LVTMDKFQQVAKMENFQKAASNPEIWKQVAVERTQSAE"
     gene            complement(15665..16456)
                     /locus_tag="VFR25_00155"
     CDS             complement(15665..16456)
                     /locus_tag="VFR25_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939488.1"
                     /translation="MRDDEFDRLVNDELDGVATPEQKAELARRLAEGEAARARYHEIQ
                     TVFGMLDRIESIDPPPSLRGNVLRAVESRARTRSERGGWRGLILAGFGKRPGLGLGYA
                     FAVGAVVGAVALAIGSGLFDRAGLRGPEMQGALLPPTGSRVDAIHLAAKGAQATGEMW
                     TEFNGGVSVRLEVRSDVPFEAHVKFNPEIYIPVSLRRVEPSGGRFDLRRGELQVSDRG
                     SARFELRLRAVTASRPPLSVWVGTSGGSAEGQLEPRPPNDPKIRR"
     gene            complement(16453..16992)
                     /locus_tag="VFR25_00160"
     CDS             complement(16453..16992)
                     /locus_tag="VFR25_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9939489.1"
                     /translation="MSDDEELAAVERCLRGESRAFETIVDSYQRVLFNVALRMVNDRD
                     DAQDVVQSAFVKAYEKLHTYDRRRKLFSWIYRITVNECLDQLARRKRQGPMDEDEPGR
                     GPEDALEEREVATVIGAALLDLSVDYRGVIILRHFVHLSHREMSRVLEIPEKTVKSRL
                     HTARKLLGELLAKRGVSRA"
     gene            complement(17027..17308)
                     /locus_tag="VFR25_00165"
     CDS             complement(17027..17308)
                     /locus_tag="VFR25_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9939490.1"
                     /translation="MLEIKVENQSVKLKGRLDAASAPKAREALRPLKGPMALDLSDLE
                     YISSAGIGVVVETYKRLHGEGAPLRVLNLTPGVRAVFRFAGLEQMLAIE"
     gene            complement(17301..17711)
                     /locus_tag="VFR25_00170"
     CDS             complement(17301..17711)
                     /locus_tag="VFR25_00170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9939491.1"
                     /translation="MERRFQRDLSSLPELSRFVADFLSHHGLSLSNAYDLDLILEELF
                     TNVLKYSSGTDEVAVGLTRDDSTITIALREFDVAKAYDITRAPDPDLTRPIAERKVGG
                     LGIHMVRQLAETIRYEYKDRVSTTTITKKVSSNA"
     gene            17860..19635
                     /locus_tag="VFR25_00175"
     CDS             17860..19635
                     /locus_tag="VFR25_00175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9939492.1"
                     /translation="MDLVLEGTVKGQYVRIHLDKPALRVGRSQDADIRIDDATVSRSH
                     AILSVLDDHLELKDLGSHNGTYLNGRRLTDSAAVKPGDQIRIGSIVLTLLPTAAPTMG
                     IQQIATILLPAEAVRTVMQVPLEVIRERKNTKTDPENLPTMIGSRLMDLMETVPEEAD
                     TYEGHLEVVTTALPRMIATQTPDEAIQTLPELVQCMGPHRTLTLLLYDTTRREMYLKA
                     AWPAKALESRKTLLTADLLRTAMNEKMSHLVERVGGGTTIDGATGDGLPRDGHGGEIG
                     DALDRVAFVAPLRVNEEPVGILYVGRSDLDENGTRTALHAFNSLASLFAMKMAQCMEF
                     ERRLREHAQLQREKDRMSDAIAIAARIQKKLLPERLPEASGYDIVADLWPSLETSGDL
                     YDVLRLSSSQVGLVVGDASGKGLGAALLMANVLAPLRMSFTESASLVEIMERLNAQLE
                     QSTDPDHFVTLFLGRLDTERHTLEYVNAGHIFPVLLAPGAKAVRLEATGIPAGMMAGT
                     QYGSRTVDVPAGALLCLYTDGMPEAARVGGPEYGEERLHSMLARSTSRPASDTMAELR
                     KDLDEFMAGEPLADDTTILMVRRTR"
     gene            19632..20117
                     /locus_tag="VFR25_00180"
     CDS             19632..20117
                     /locus_tag="VFR25_00180"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET9939493.1"
                     /translation="MTSTLVRLMLLAACVLAWTSCQRDRSQTSERRQVERPDTAGIST
                     PTPPEPPTPPAKQVIVDSPESGALITSPVLVTGKARGSWYHEAEFPIRVLDSQDKMVG
                     TGVARAKGEWMTSDFVPFEASIVFRAPPEESTGFLVIEKSNPSGSPEHAAESRVPVRF
                     R"
     gene            20118..20891
                     /locus_tag="VFR25_00185"
     CDS             20118..20891
                     /locus_tag="VFR25_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9939494.1"
                     /translation="MADTSATPPRNRRKVLVVLPVYNEEAKIGSLLDHIDEAMRDADI
                     RYEVVLVDDGSKDGTDAIIRRRAAHMPLMLMRHEVNKGLGATIRDGLFAAAEAAGEAD
                     IIVTMDADDTHAPGLVLRMVRMISEGYDVVIASRYREGSRCVGVPLHRLALSHGASWL
                     FRVVFPIQGVRDYTCGYRAYRAAVVKAAFARYGEEFLDQGGFQCMVDILLKLRRMNLI
                     FGEVPFILRYDWKEGGSKMNVTRTVKDTLGLMWKRRFGG"
     gene            20888..21541
                     /locus_tag="VFR25_00190"
     CDS             20888..21541
                     /locus_tag="VFR25_00190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9939495.1"
                     /translation="MSEARFEQEYYASVYPDYARQNPARKLRFYRGLVERVAPVRAPV
                     RILDIGCAFGAFLSALDPRWERYGTDVSRYAVEKAAAALPEIHFARTDIDAIPFPGPF
                     DMITSFDVIEHIPSLDAVASAVESRLAPGGHFVFVVPVYDGVTGPIIRLLDKDETHVH
                     KRSRDFWLDWARGRFEVVAWCGIYRYLVPGFGYAHLPTRRLRRFTPAIAVTARKRDR"
     gene            21567..>22143
                     /locus_tag="VFR25_00195"
     CDS             21567..>22143
                     /locus_tag="VFR25_00195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9939496.1"
                     /translation="MSRRQGRASKPGRKPERSAGPRRPDRRRVWTFVAIAIIVLVSLA
                     RAIHLNFPLERDEGEFGYIAQQLLHGVPVYLSAYTQKLPGAYYVYALFLLLFGQSTVG
                     IHLGFLLANAATMALVFLILRKTHGGLAGCAGALVYGVMALSPALLGSSGHATHLIAF
                     FSVLGLYALLEARERGGLWLYLVSGLSFGMAL"
BASE COUNT         3668 a         7380 c         7487 g         3608 t
ORIGIN      
        1 gagcacctga tccaggaggc ttgtatacga tgaagatcga gcgttcgttc actcgggtcg
       61 cggctcggcg cctcgttcgg gccctctcac tcgccgggct cgccgcgctg acggcaggat
      121 gcgccgccac ctcggtcaag ccctgggatc gcgatctcct cgcccagaag aagatgcagc
      181 tcgtccccca tccgatggtg aacgccatcg acgagcacat ctatttcagc aaggaaggct
      241 cgaccggcgg cctggacgtc ggcggcggcg gctgcggatg caactagctt caccgggcca
      301 agcctccatt cgcggcaagc tgggaaccgc ggcctgcatg ctgctcgcgt cggtggctcc
      361 ggccgccgcg cgcggagatg catcgggagc cgtgacccag gtcgacgcga ccatgctcct
      421 ctacggcgag cagagccgga cgaacgtcat cgagcccacc gtgcgcgtga cgcgcatcca
      481 gccgagcggc cgttcgctgt cggggcagat cgtcctcgac gcgatgaccg gcgccacgcc
      541 gaccggagcg atgcccccgg gtgtcgtgga ctcgaccgcg acgttgcccc ccggcgagga
      601 ggcgcggcgc gtccagacga tgacggcggc ctcgggcggc ggtggcggcg gatccgaaca
      661 cgcgcccgat ccaggcgcga ttcccctggc ccaattcaac gacacccgcg ttgcgctcga
      721 tgccgagtgg cacgagccga tcacgagcgc gttcggatcc acgctgggcg ggtattactc
      781 gcgggagaag gactacgagt cgatcggggg gagcgccagg atatcgctcg acttcctgca
      841 caagctcacc acgctcacga tcggaggcag cgcgaaccag gaccacgtct tcccctccgg
      901 tggaacgccg ctccccctga gcgatggctc ggtgctccac gaggagcgca acgcgaagca
      961 cgtgacgggc ggactggtgg gactgtcgcg cgttatcacg cgccgctggc tcatttccgt
     1021 gaacggctcg aggacgtacg aggacggcta tctgaccgag ccctacaagg tggtgagcct
     1081 catcgatccg gcgaccggct ttacggcggg acagatcacc gaggggcgcc catcgacgcg
     1141 ggagcggtcc gatgtgatga ccacgatggc ctatcacttc agaagcgacg tgctccatct
     1201 gtcgcaccgc tattactggg acgactgggg cgtgcggtcg aacacgttcg atctgaagct
     1261 ccggcacgac accggcgaca gctcgttcct gcagccgcat ctccggtact accagcagag
     1321 ccaggcggac ttcttcacgt tcggcctggt gcagggagcg ccgattccgc agtacgcgtc
     1381 gagcgactac cggctgggtc cgctccagac cgcgaccgct gggttgacgt acggcttcca
     1441 cctcccggag tcgccgggcg agttcacgat ccgggcggag tacatcctcc agttcgggaa
     1501 gggacacccg gacaatgcgg tgggcgtgca gcaggacttc gatctcatgc ccccggtgaa
     1561 catcggctcc ctggtcgccg gctacagcgt ggctttctga tcgacagcgc catggccaga
     1621 tccgcaccat cgccccgccc cgctggcgaa tccttcgtcg agcggggcga cggctgtctg
     1681 gtaggccgct tccgagccat ggcgagcccg tgcgaggtgc tcgtcgacac ggacgacttg
     1741 gacgaggcgg cgcacgcact ccggattgcc gaaaccgagg cgcaacggat cgaggcgaag
     1801 ttcagccgct accgcccgga caacgtcgtt caccggatca accatgccgg tggcgtgccg
     1861 gtgcgggtgg acgcggagac cgcgcagctc ctcgactttg cggcgtcgtg tcatgagatg
     1921 tcgggcggcc tcttcgacat cacgtcgggg tcccttcgcc gcgcctggac gttcgacgga
     1981 ggcacgcgcg tccccgaagc gtcccaactg cgcgcgctcc tcgagcacgt cggatggtcg
     2041 aaggtgcgct gggacggcac ggccatcgcg atgccggcgg gcatggagat cgatctcggc
     2101 gggttcggca aggagtacgc ggtggaccgc gccgcggggc tggttcgcga cgaagtgtct
     2161 cgcgcgatcc tggtcaactt cggcggagat ctcgtcgcta gcggggcacg gcgggacggg
     2221 cgtccctgga gcgtggggat cgaggatccg gaacgacccg gtgcggccgc tctctatcga
     2281 atcgaggtcg atcggggcgg catcgcgacg agcggggacg cgcgtcggtt cgttctatgg
     2341 cgcgggaaac gcctcggcca catcttggat ccccggaccg gctggcctgt cgagggcggc
     2401 ccgcgatccg tcaccgtgct cgcggccacc tgtctcgaag cgggaacctt ggccaccctg
     2461 gcgcagctcc agggagccgg agcgcgggcg tttctcgatg cccagggcgc tcggtactgg
     2521 acggtgtgac agacacggcc cgaaggtcgc tagaccgcca ccgcctgacg tagatcctcg
     2581 atcagatcgt ccgggtgctc gaggccgatc gagacccgga tcaaatcatc cggagtcggc
     2641 gagtccgggc tctccacgga tcggcggtgc tcgagcaggc tctccacgcc gcccaggctc
     2701 gtcgcgttcc gaaagagctt cacccgcgag caagcacgca ccgctccctc ccgtccgccc
     2761 gcgattcgaa gcgagagcat cgccccgaac gcgctcatct ggcccttcgc cacggcatgc
     2821 cccggatccg actcgaggcc ggggtaatgg atcgagcgga tgccccggtt cccctcgaga
     2881 gcgcgcgcca cggcgagcgc gttggccgac tggcgctcca tgcggcacgc gagggtccga
     2941 aggccgcgca gcaccatcca ggacgcgaac ggcgacgcga cggcccccag gatgtggcgg
     3001 ttgtgctgcg cgcggtgcga gagctcgccc ttggtccgga agacaagggc cccgccgtgc
     3061 acgtcgctgt gtccccccag gtacttcgtg gtcgagtgga gcacgatgtc cgcgccgagc
     3121 tcgagcggac gctggagcgc gggcgtggcg aacgttccat ccacgagaag gagcgcgccg
     3181 gcccccttcg ccagcgcggc gatggcgcgg agatcgagga tctgcatcgt gggattcgtc
     3241 gggctctccg tccagacgag ctgcgtgttc ggacggagcg cgctccggag cgcgtcgagg
     3301 tcggtcatgt cgacgaacga agcttccatg ccccatcgcg gcacgtactc gagcgccatc
     3361 gtgcggaagg cgtagtagag gtcgccgtgg aagaggatgt gcgctcccgg ctcgagtgcc
     3421 tggaggcacg ccgctcccgc gcccacgccg gagccgaacg cgagcgccac ttccccgccc
     3481 tcgaccgcga cgagtgcttc ctcgagccgg gactgcgttg gattggattc ccggatgtag
     3541 atgtgccccg cggggcgcgg ctcgccggcg ggaccgtgct cgaacgtcgt ggagagatgg
     3601 agcggcggcg cgatcgcgcc ggtcgccggg tcgggatcac cccctgcgtg cacggcgcgg
     3661 gtctcgaatc gcatcggcgg atgctgcacc ggctcacgcg gcgggatcaa gaggtctctc
     3721 gctccggggc cgacctggcc gcgcgacgct gggcctcccg cgcccgcagc gcgcgtcccc
     3781 caatggcgag cccgacaccc cccaggatga aaatcgcgga gagcaccaag cgccgcgtgg
     3841 cctgctcccc cagaagcgca atgccgccga cggcggcgat cagggggacg gtgagctgga
     3901 gcgccgccgc ctggagcgcg ttcagcctgg gaagggccgc gaaccacgcc gcgtagcccg
     3961 ccccggaggc caggacgccg gacgctgtcg caaggagaag gccgtgcgtc gaaagatgga
     4021 ggtgccccag cggcgccgcc accgcgaacg cggccagggc catcggcgcc gagcgggcga
     4081 agttggacgc cgtgtcgccg atcgcgtcgg tcgagcggcg gccgcgcaag gaatagaggc
     4141 cccacgcgac ccccgcgagc gccatgagcg cagccccctc gggggacggc gcggagaggc
     4201 cgggcgccat caagtgagcc agtccagcga gggcgacggc gatgccgttg acctcgagcc
     4261 agtgaattcg ctcccccgag acgaccgcgc cgacgagcat cgtcgcctgg acgcagaaga
     4321 agagaatcag cgcccccgtc gccacgccga gcgacacgta ggcgagagag aacggcagcg
     4381 cgtacacgaa gaggaggagc gcggatcccc acgagcccgc gtgcgacgcc ccgcgggact
     4441 tcctacgacg cgccctcacg atcagagcca gcgcgatcgc ccccgaagcg aggcgggcca
     4501 ccgtgaagga ggcggggtcg atcgctcccg gctggagcgc aaggcggcag aggatcgagt
     4561 tcgccgcgaa gccgacgagc gcgaacagcg tgtagagcgc ggtcacgaac cgcccttgca
     4621 aggcgccatg gggcggaagg atagcaggcg tccgcctcct tgacctcatc cggacgtgcg
     4681 actagccttg aggtctaccc tcggaggaac tgtgacccaa gacgacgagc acgatctggg
     4741 ccacctggtc gtcgtgggtt cctcggcagg cggcatcgag gcgctcggtg tactggtgtc
     4801 cggcttgacc cctgatttcc cggctccggt cgttctggcc cagcacctcg atccctcgcg
     4861 cccgagcatg ctcccgggga tcctcgagcg acgcaccaag gtgcccgtcg agaccctcac
     4921 ggagaacacc aagctccagc ggggtcatgt ctacgtggtt ccgtccaacc ggcatgtcgt
     4981 catccaggac gggacggtcg gtctcgagca ggataacgac gatcggccac tcccctcgat
     5041 cgatcttctc ttctccaccg ccgcacgcgt ctatcgggag cggctcatcg cggtgatctt
     5101 gaccggctcg ggctcggacg gagcggatgg tgccatcgag gtcaaggagc ggggagggtg
     5161 cgtcgtcatc caggatccgc gcaccgcggc ccatccctcc atgccctcgt cccttccccc
     5221 gaccttggtc gatcacgtcg ccgatctgga caagatcgcg cccctgctcc aggacatcct
     5281 gaagggcacg acgatcgaga ggcgcatcga gaccgcgcac tcgtcggatc tcgacgagat
     5341 cctcggaatc ctgacgcggc agggaagcat cgatttccgc cagtacaaat cgaccacgat
     5401 cctccgccgt ctcagccgcc gcatggcgct caaccacatc cacgggctgg aggaataccg
     5461 cgatttcttg gccttgaacc cccaagaggt cgcgatgctg gtccggtcgc tcctgatcaa
     5521 ggtgaccgag ttcttccgcg atgccgacgc attcgccgtg ctggagcggg agatcatgcc
     5581 cgagctcatc gagcgtgggc gaggacgggg tcgcgtgctc cgcctctggt ccgccgggtg
     5641 cgcgacgggt gaggaggcct attcgttggg gctcatggtc tcccacatgc tgggccggga
     5701 gctgccggag tggagcatca agatcttcgc gaccgacgcg gatggggacg cgatcgccta
     5761 tgcgaggcgc ggtgtctacc ccgccaccgt gctgcgcagc ctgccggccg agtacaaggc
     5821 ccgctatttc gagccgctcg accaggggct gcgcatcagc aaggcccttc gacagatgat
     5881 catctttgga cagcaggacc tggcccaggg cgttcccttc cctcggatcg atctcgtatc
     5941 ctgccgcaac ctgctcatct acttcaaacc cgagctccag caggcggtgc tcgatctgtt
     6001 cgcctattcg ctccagcaga cgcacggttt cctgttcctc ggcaaagcgg agacggcacg
     6061 cccgtccaga tcgacctacg agatcgtcaa caagaagtgg aaggtctacc gttgcttgag
     6121 cggcccgctc ccgatcgcgg tccgcccgcg cggcgagaag ggacgcggcg aaagcccggc
     6181 cgtgtctgga cccgcgacgc tcaacgccga gacgctccag gcggcgggag cgcgcatccc
     6241 cgaggaggtc gagctcaggc gggtcaacga gctcttcctg cgatcgcttt ccgtgggcgt
     6301 gtgcgccatc gaccgctcct acaagatcct cagtgtgaac gcgatggcgc gacggttatt
     6361 cgggatccgc gaggtggcca cggatcagga tttcctgcac accgtgcaag ggctgccgta
     6421 cgtcgaaatc cggaatgcca tcgaccgctg cttccgcgag cggaccgtga ccgtgctccc
     6481 gcaagtggag ctgaccgaca gcctgggcga ggcgcgcttc ctggccatcc agctgattcc
     6541 cttcgaaggt ctcggcgtcg agaccgccct gatctgcgtg gagaacgtca ccgagctcgt
     6601 ccagacgcag cggcgtctgg aagcggtcca gtcggagcag agccagctcg cccaggaact
     6661 aggcaccgcc aatcatcgcc tcatggacat gaatcgggat ctccaggacg cgaacgagga
     6721 gctccaggcc accaacgagg agatgatgct cacccaggag gagctccagg ccaccaacga
     6781 ggagttcgag gccacgaacg aggagctcca agccacgaac gaggagctcg agaccaacaa
     6841 cgaggagctg caggccacga acgaggagct cgagaccacg aacgaagagc ttcatgcgcg
     6901 cacgtccgag ctccaggagc tgacgcgggc cctgtcgaac gagcgcagcc gtctgactca
     6961 gatcgtcgag caggccccct tccacgtcct cctgttgcgc ggccccagcc tgatcgtgga
     7021 ggacatcaat ccgcccctta tcgatctgtt ccccggaacc aagagcgggc agacctcctt
     7081 cgaggatgtg accaccgacc cggctctcct tccggttcgc gccggggtgc ggaacgcttt
     7141 cgtcaacggc cgcccgtggc agagcggctg gatccgggtg ggacagccga tggacctgcg
     7201 cttctatcag ttcaccgcgg ttccaacgca cgattccgga ggcaaggtcg acggcatcgt
     7261 gctctacgcg gaggacgtga ccgcacggcg gatccatgag gaggcggaga agctgtccag
     7321 gctcaggctg atgctggagc acgcgagcca gctcgccatg ggtctgttca atgccgaggg
     7381 caagctggtc cacgcgagcc agcagttcct gggaatgctc cagcggctcc ggaagatcga
     7441 tccggcccat gctatcggca tggattggga gcgcctctgg ttcggagcgc ccgacctgct
     7501 cgacacattc cagaccgcgg taagcgaggg gcgggcccag cgactcaacg aggttcagat
     7561 cgaaggcgac ccgcagcgcg cggtctggga ttgcagtctg atcccgatca cacccgaagg
     7621 ggggggagcc gtcgatttcg tggtcgtcac ggccgtggag gtcacccgtc cggtcctggc
     7681 ccgtgaggcg gtcgagcagg tggaccggct ccgggacaac ttcctctccc tcgcgagcca
     7741 cgagctcagg accccgttga cgcccctcgc cgcctacatc gaggtgctcg cccatctgct
     7801 cggcgagaag agccggactc cggactggga gcggcaagtg caggacgtca tcggcaaatt
     7861 ccgccgccag atcggataca tgtcgcgtct cacggaggac ctggtcgaca tctcgcgcat
     7921 ccggtcgggc aagctcaccc tggatcggag gccggtcgat ctgaggcacg ccgtcgaaga
     7981 ggggcgggat caggcgctcg ccctgggcgc aaacgcgcgc gtccaggtga cgtccgccga
     8041 cgccgatctc acggtgcacg gcgacgagat gcgcctcgcg caggtcgtct acaacctgct
     8101 ctccaacgcc atgaagcacg cctccgcgag cgagcgcatc gaggtccggc tctccgagaa
     8161 gcgcgacaac ggccgagcct ggggacgtgt cgaggtgtgc gacgagggtc ccggcatccc
     8221 cgaagaatat cgggacgatc tcttcctgca cttcgtgaat ccgaccagac gtgagggtcg
     8281 ggcggcccgg tccggcctgg ggctggggct cttcatctcg gcccgcatcg tcgagcagca
     8341 cgagggccgg atcggcgtcg aggacctcga gcacggcaca tgcatctggt tcgagatccc
     8401 gctgatcgat gctcgcgtcg gagcatccac accgcctgcg ccgcggaatt agctcggcgc
     8461 cacgcccgcg tcagtgctcc cgagccacct cggacgggcg gcaggcgaac aggatcccac
     8521 gaagctccgt ccaatccgtc ggctttcgga gcacggccgc cgccccgagc tggcgcccac
     8581 ggtcgaatac ctccgcctga ccggccgccg agtggatgac gatcgaagga gtgggtcgaa
     8641 tccgccgcag atgctcgatg aggaccgagc cgtccatgtc gggaagcatg agatcgagca
     8701 ggatgacgtc gatggcgggg ccgccgttga cggcgcggaa gatgtcgatg gcttcccgtc
     8761 ccgtgcgcgc ccaatccaca tggatccctt cgctccggag cagaagggac atggcctcgc
     8821 cggagtcgac gtcgtcttcg atgaggagga gctgcagagg tcgcatggat tggcgtccct
     8881 gggtgtgtca gcgcggagtc aattgtggtg ggaaccaacg gcgccgacca acctcaaact
     8941 tcacgttcgg gaggatgtgc ccgtttttgt aatcagcggc gcggtcttga aaagacctta
     9001 tcccattggg agagcgccgg tttcggattc aagtgctcgt ccaccagtcc gaggtgcgcg
     9061 aaggggccga gactcgtccc cggtggaacc gggaaggaat cgacgtcgag atccgtgaac
     9121 gtgatctgga accacgcgcc cgcctccgcg cgatccagga tcgccgcgtg ccgcgcgatg
     9181 tagcgcgcct gcttctccgg cgtccccgcc acgcccatgg aatcggacgg ccatccaccc
     9241 tcgatcacca tcaccggaat cggattcccc gacgtgagcg ccgtgtagta gtcgagcgga
     9301 atgctgtcgg ggtcggtgaa gccgccgaga tacggatacg acgacagtcc gagcacctgc
     9361 atgaacggaa agtccgtccg gtcctgggcg acgccgacga aggtgcccgg aggaaggagc
     9421 tttccccagg ccgtctcgac ctgaacggtg ctgaagagct gcacgttggg atcgaccgca
     9481 cgcacgtccg cggccgcacc gttcgccgcc tggacgacgg cgttgtagac cgacgccggc
     9541 gcgatcgcgc gcacgagatt cgtctcggag gcgacgccga tgtagtcggg gtgcaggatc
     9601 gtgtccatcg ccgtcacgta cgcgcggtag agctgttgca cggcgggctc cgcgaggctc
     9661 ctccccgcgt cgacgagcgg ctgcgactcg gcggagcggt tgagcccgtt cgtgggatcc
     9721 acgctcacga tgatctggag ccccttcgcc cggtagtagt tcaccaggcc gagctgtgtc
     9781 gcgcgcacgg cggagtcggc cggaattccg gagaggagcg cggcccaggg gggctcgctc
     9841 aggacgagcg ccgcatccgc gcgcaaggac cataggttga tcgtctggat gacgaggttg
     9901 aagtcgttgc gcggggggat acccgagaat cccatgcgat acgagcgcgg ctcgaggctc
     9961 gggccggtcg gattgtcgtc gccggagcac cgcaccgaga ggacggcggc caggacgagg
    10021 gcgagaacga tgcgttggcg catgaggccc ccaccataac cggaccgggg cgcgagggcc
    10081 agagggagac cggaacgact agcgctcgag cgtcgagagc tgccactcgt cccggccgcc
    10141 ttgacgcagg accggcgaca tggccatgag tccggtcggg ccgatcgctt tcttcaagaa
    10201 ctccatctcc gcgagatcgg cctccacgtc gtcgagcggg atctccacgc ccgcgcttcg
    10261 cgcgacgagc ctcgctttcg cgcggagacc cagctcggcc tcgatccgga gcaggcagag
    10321 catgtccgcg accaccgtgc ccggaaaccg gtggcggatg gattcgaggt agcgccccac
    10381 ccgggtctcc cccagctctc cggtgaggat ggaatcgagg agctccaggt cgctgtcgaa
    10441 ctccgagctg agccagtgct gcgtgacgcg ctcgcttcgc tcgaacacgg cgatgacgag
    10501 gggcggcatg aggaggcaca tgatcgcggt cgcgaggagc gggttcacga agaactggtt
    10561 gtagatcgcg tgcgacgcga tcgcgatgcc tagcccgggc aggaagaccc accacgcgga
    10621 ggagcgatgc cggtcggcga agcttttcga gaggataccc acgatggcgc tggtcgagcc
    10681 gtgcatgatt gcggtgccga gcccgcgcac gagccagagg agcacgccat agtcccccag
    10741 cgcgcgggcg tagtagaggt tctccaccac cgcgaacccg gtccccaccg caaaaccgcg
    10801 aattcccgcg tccaccatga agccgacgcg ctgcgagcgg acgaggtaga tcagataggc
    10861 ggccttcgcg atctcctcga ttaggggcgc ggcgtagctc cggagcgtgc cttcgtcgag
    10921 cacggcgccg aggaggctgc ggttgatcgc gaaggacacg atcgccgcga cggccccgag
    10981 ccccatcgtc accgcgatgg cgcgccccgt gaccagcttg tagctgtcga ggaggacgag
    11041 gccggtcagg aagaggagaa ccgggaggaa gccgaggacg atcggcaggc cgtcgactaa
    11101 gtgcgcggga gccacgcggg tcagcctacg ggagcgcggt cgtggatcaa agaaccttga
    11161 gcgagatcat ccactcgtcc gacgtgcgct cgccgtcctc gaatccgcgc gcgtaccctc
    11221 cggagagcat gagcttgtac tgggtgagga gctggatctg gaggtccgac tggatgccga
    11281 cgtcgaacac ggtgcggcgc agcgacggga ggtccatgtt cgtggccagc ccgcccgcga
    11341 agagggaggt tcgaagccac ggcgcgtaca gcgcggacgt gccgaggcgg cggaagcgga
    11401 gcggcgccag gttccaatcg acgagcgcct tcgcgaagtt cgtgccggcg atctcgttca
    11461 gctcgaagcc ggggaagctc tcgacgtcgc ggtagcgctt cggctccttc cggtcgatgt
    11521 agttgttccc gaagccgccg aaatagaagt tcgcgaacgg ctcctctcgg tcccccggcg
    11581 cgaagccccc cgcggtgcgg agccagatcg acgagtgcgg gaggagcatc ggcgtgccga
    11641 cgtcgagcgt ggcgagaccc tgcgggaatc cctgccagac cgtgccctcg gggcggtcgt
    11701 agcggacgag attccacggc atgccgagcg accacttgta ccccttctcc cggtccacgg
    11761 cgccgaggga ggcgcgcgtg ttctcgtagc ggaggccgag atcggtggtg acgaggctcc
    11821 ggaacccggg cgatacgtcg atgttctgtg cgtccggaag ccgctcgaga tcgacatagc
    11881 ccgcggccga ggcgtcgaag gtgagcgacc ggggcgcgtc atagaggagc gagcgcgtat
    11941 acgacgcgac gacgccctcc cctttccggc tcgtcttcgt cggtccgacg aggtcgtaga
    12001 agttcgccag attgtgctga tagctcagct ggaggttgta tcgcttgtag cccgcgagga
    12061 cgtgccagcg ctcgttcgcg ggaagtccct cgctcggtga gtacgaggcc gtgaggccga
    12121 gcgtgttcgt ctggattgga tccgagagct cgagccgcat cccgacggcg gtgtagtcct
    12181 tgtacccctc cacgatcgga tacagagagg acgggcggat gaggctcacc gaccggtacg
    12241 gccccgaata ggtggtgatg gagtccaggt tcaccgtcgc cggcgatccc gccttccacg
    12301 tggtcaggac cggatacttt tccgcgagct gggttccgag gaacgagatc gcgctcacgt
    12361 cggcgagcgg tttgggatcg atcatcgtgg ggacgaagcc ctcgcccgtg tatcgaaacg
    12421 cgatgagcga gtcgccgtcg atggggagcg ggcggaacac ccctgtctcg ctgttcgtca
    12481 ccacttccat cgaatccgcg gcgaagttcc agcggaagat gttcgagacc cccgtgtagt
    12541 acgagctccc caccagatag cgcccgtcct tcgagaagac gaaccccgcg ggcacggcgg
    12601 agccgaagtc gtagagcgtg gtcgaggtgg tgtcctgcgc gaggagcgcc tcggtgcgga
    12661 agatcttgag cgactgggtg ccgtcgatct ccgtgcagga ggccgcgagc ttggcgccgt
    12721 ccggcgagat gtcgatgtcg tagatgtcgc ggccatacgg ccacgagtag acgcgattcc
    12781 agttggtgta gggcggctgc aggcgaacga gcgtggacac cccgttgaaa tgccggaccg
    12841 cccacatggt gcgatcggcg ggattcagga ccatgtcccc gagtctcgcg tcccggatca
    12901 acacccgcga cttccccgtc ctcggatcgg ccacgcagag gtcccgccac ttgctgttgt
    12961 ccgccgtgta gtagaggagc ttgtcggccg gatcgtaggc gagcgacgtc acgaagtaga
    13021 gcgccggtcc cttcacctcg cagatccgcc ggagcggccg cccatcggtc gggatcgccg
    13081 cgatgtgcgc cacggcgccg ggatagagga ccgcggcgta gatctcgcgc cggtccgggt
    13141 cgtagtaggc gcgggagatc gagccgagcg cccggggcga gagatcgcgg tacggcgtga
    13201 tcgggtggag gtcgatcgag tcgagctgcg ccgtctgctc ccccttttcc cagtcgatcc
    13261 agttccgcca cgccgtccca agcgggaggc catacacctt gtgaaattga gacgcgtagt
    13321 aggccttgct ccccgagccg cgcccgaccc actggaccag cttctcgggc tcgtactcgt
    13381 aggcgaggta gctcaggaag cgcgtgccgt agaggtagga gttcacgccc gcctggaagt
    13441 cgaccttcgt tccctcagcc tcgagcccca gcgggtcgta gatcgtgctc ccgtccttca
    13501 ccttcgagcg gaacaccatc tcgtcgtacg ggccctgcgc acgcccgagg ccgccggcca
    13561 tccaggtctc catgaagacg gcgatcccct cgtggtacca gcgcggcgcc gctcgacggg
    13621 gcgtggtgag gtagccgtag agcatcgtct ccggatgctc gttcgcctcg cgcaccttgc
    13681 cgcggaagag cgagcggaag aagcgatccc gccctgacgg cttgtcgagc gcgaggacgt
    13741 gcaccacctc gtggttcatc gtgtggttga tccgctcgtt ggccggcccg gtctcgtaca
    13801 cgaagttgag cggcgcgatg tccacgacca tgctgtttcg gggattcacc cataccccgg
    13861 cgtttccgta gtcggccaag tcctcgagga tgacgttgac cttctcggac ggggtgtagt
    13921 tccagagctt gctgtggaac cggagggagt tctcgaagca gcgggccgtg tacggagcga
    13981 tgaaatcgag ggtggggctg agatagatca gccggaggtg gtcggtctcg atcgacttga
    14041 gctccgctcg cgcggggcgt ggggccaggg cgaggcacgc gagcagtccg aggaggagcg
    14101 aggcacggcg cgctgcggcg gctcttctgg ttctcatgcc tctccgttcg ggtggaatga
    14161 agatcggtat ccggacaaac agcgaggaga tcccgcgtcc gggatctcct cgcctttaca
    14221 aggccgcggc tctcaccggg agccgcgccc cctccattta ctcagcggac tgcgtgcgct
    14281 ccaccgccac ctgcttccag atctcgggat tgctcgcggc cttctggaag ttctccatct
    14341 tagcgacctg ctggaacttg tccatcgtga cgagcttcgc aaactcattg ctcttcgcca
    14401 cttcgcggaa gttcgcgtcg gcgaacacgg tcttgaactg ctcggaccgc gccagctcct
    14461 gcgcctgagc gtaggcattc gcgtcggcgg tgcggccggc ttcggcctga accgtgcgcc
    14521 ctgcttccgt cgtcgcggcc ttgtcgaggg ccgtattcac cgtgcgctcg taggcctggt
    14581 tggccttcat gtacatggtc cgggcggcct cggcagaggt ccgcgcctgc tccgtcttcg
    14641 cgacctccgt cttcgccgca ctcagcttgg acagctcggt caccgacttc tggtacatgg
    14701 tcgacgtctt gtcgagtgca tcctgggcct tcgcgagctc ggccttggcc tgctccacct
    14761 tggccagatc ggtcctgttc cagccggcct tgccgatgtc cgtcgtggcc ttggccagat
    14821 cggccttcgc cacctcggcc ttcgcagcct cggtcttggc ctgctccatc ttcgagagct
    14881 cggtcacggt cttgtcgtac gcggcctgtg ccttcgccag ctcggtgcgg gccatgacgt
    14941 cctgcgcctt cgcctgctcg accttggcca cttcctgctt gttggccgtt gagctcgtct
    15001 tcaggagctc gctcacgctc ttgtcgtacg cggcctgggc cttcgcaacc tcggtccgcg
    15061 cgttcgtcac ttgggtccgg agctgctccg cgttggcgcg atcgagcgtg ctctgcgtgc
    15121 gctccgtgag ctgcgtcctc tggaagtcgg ccgggctcgc cttggccagg aggtcatacc
    15181 ggttctgctc cacggcggtc ttgaagagat cggaccgcat cacttcggcg aatccctgct
    15241 tgcccatcag gtcgcggaac tgcttgtcga ggagcaccgt ccggaacgac tcgctctgcg
    15301 tgcgcatgac ctcgcggaag ttcgcgtcgg tcacgagctg gtggaacacg tcgctctgga
    15361 gcaggtcctg catgtcggcg tccgtgagcg tcacgtcacc ggccgagatc tgggtcgtct
    15421 ggtaccgctt cgcagctccg atcgtgccca tgaccccgtt cttcggagga atcacgccgg
    15481 tgaaggaggc gattcccagg gcaatggcaa tgacgacgcc caatccgatt acgatccgct
    15541 tggtggacat ctcgaatccc cccttcgttt ctagacgccg cgcgtcccgc ggccggtggt
    15601 gcgcgttgat tccgaattgc gtgcgacagt acgggtgaag aacatcaagg ttgatgaaat
    15661 tctgttaccg ccggatcttg gggtcgttcg gaggccgggg ctcgagctgg ccctccgcgc
    15721 tcccgccgga ggtcccgacc cacaccgaca aggggggccg gctcgccgtc acggcccgta
    15781 gccggagctc gaaccgggcg cttccccggt cgctgacctg gagctcgccc cggcggagat
    15841 cgaaccgccc cccactgggc tcaacccgcc gaagcgacac aggaatatag atttccgggt
    15901 tgaacttgac gtgagcctca aaggggacgt cggatcgcac ttcgagcctc acggagacgc
    15961 ctccgttgaa ctctgtccac atctcgcccg tggcctgggc gcccttcgcc gccaggtgga
    16021 tcgcgtccac ccggctcccc gtcgggggga ggagcgctcc ctgcatttcc ggtccccgaa
    16081 ggccggcgcg gtcgaagagc cccgagccga tcgccagtgc aaccgccccc acgacggcgc
    16141 cgacggcgaa ggcatacccg aggcccaggc cgggccgctt gccgaagccc gccaggatga
    16201 gaccgcgcca gcccccgcgc tccgatcgag tcctcgcccg agactcgacg gcgcgaagca
    16261 cgttcccgcg aagggacggc ggcggatcga tcgactcgat ccggtccagc atcccgaaga
    16321 cggtctggat ctcgtgatat ctggcgcggg ccgcctcgcc ttccgcgagc cggcgggcga
    16381 gctcggcctt ctgctccggc gtcgccactc catccagctc gtcgttgacg aggcgatcga
    16441 actcgtcgtc tctcacgcgc gcgatacccc ccgcttggcc agaagctccc cgaggagctt
    16501 gcgtgccgtg tggagcctcg acttcaccgt cttctccgga atctcgagca cccggctcat
    16561 ctcgcggtgc gacaggtgga cgaagtggcg caggatgatg accccccggt agtcgacgga
    16621 gagatcgagg agcgccgctc cgatcactgt tgccacctcc cgctcttcca gcgcgtcctc
    16681 gggaccgcgc ccgggctcgt cctcgtccat ggggccctgg cgctttcgcc gggccagctg
    16741 gtcgaggcac tcgttgaccg tgatgcgata gatccagctg aagagcttcc ggcggcggtc
    16801 gtaggtgtgg agcttctcgt aggccttcac gaaggccgac tgcaccacgt cctgggcgtc
    16861 gtcgcggtcg ttcaccatgc ggagcgccac gttgaagagc acccgctggt agctgtccac
    16921 gatggtctcg aaggcccggg actcccccct caggcaccgc tcgaccgccg ccagctcctc
    16981 gtcgtcggac atcaatacct gcccttacgg gaaaggttgg tgagaatcat tcgatcgcca
    17041 gcatctgctc gaggccggcg aaccggaaca cggcccgcac tccaggcgtg agattgagaa
    17101 cccggagggg agcgccttcg ccatggaggc gcttgtaggt ctcgaccacg acgccgatcc
    17161 ccgcgctcga gatgtactcg aggtcgctca ggtcgagggc catcggcccc ttcaagggcc
    17221 ggagcgcctc gcgcgccttg ggcgccgagg ccgcgtccag cctccccttg agcttcacgc
    17281 tctggttctc gaccttgatc tcaagcattc gagctcactt tcttggtgat ggtcgtggtg
    17341 ctcactcgat ccttgtactc gtagcggatc gtctccgcga gctgtcgaac catgtggatt
    17401 ccgaggcctc ccaccttgcg ctccgcgatc gggcgcgtga ggtcgggatc cggagcgcgg
    17461 gtgatgtcgt acgccttcgc gacgtcgaac tcgcgaagcg cgatcgtgat cgtggagtcg
    17521 tcgcgcgtga gaccgaccgc cacctcgtcc gtgccggagc tgtacttgag gacgttggtg
    17581 aacagctcct cgaggatgag gtcgaggtcg taggcgttgg acagactgag accgtggtgg
    17641 gacaggaaat ctgcgacgaa ccgggagagc tcgggaagcg agctcaggtc ccgttggaac
    17701 cggcgctcca tacgacggcc tcgctgtgga gtcgggtgcc gcgcaggggg agggaccggc
    17761 ggcgtggctc aagggcggcg gtaactatag gcgcgggacg aaaccggttc aatgggcgcg
    17821 acttgactaa gattccgccc ccgcccagac tccgacgcca tggatctcgt tctcgagggc
    17881 accgtcaagg ggcagtacgt tcgcatccac ctggacaagc ccgccttgcg tgtcgggcgc
    17941 tcccaggacg ccgacatccg catcgacgac gcgaccgtgt cccggagcca tgcgatcctc
    18001 agcgtgctcg acgaccatct cgagctcaaa gacctcggca gccacaacgg gacctacctg
    18061 aacggacggc gcctcacgga ttccgcggcg gtcaagcccg gcgaccagat ccggatcgga
    18121 tcgatcgtgc tgacgctcct ccccaccgcg gcgcccacga tgggcatcca gcagatcgcc
    18181 accattctcc ttcccgcgga ggcggttcgg accgtcatgc aggtcccgct cgaggtgatc
    18241 cgggagcgaa agaacacgaa gaccgatccg gagaatctcc cgaccatgat cggaagccgc
    18301 ctcatggatc tgatggagac cgttccggag gaggccgaca cgtacgaggg acatctcgag
    18361 gtcgtcacga cggcgcttcc gcggatgatc gcgacgcaga cgccggacga ggcgatccag
    18421 acgctgccgg agctggtgca atgcatgggg ccgcaccgga cgctgacgct cctcctctac
    18481 gacacgacgc ggcgcgagat gtacctcaag gccgcgtggc cggccaaggc gctcgagagc
    18541 cggaagacgc tcctcaccgc cgacctcctg cgcaccgcca tgaacgagaa gatgtcgcac
    18601 ctggtggagc gcgtgggagg tgggaccacg atcgacggtg cgacggggga tggcctgccg
    18661 cgggacggcc acggcgggga gatcggggac gccctggatc gcgtggcgtt cgtggcgccc
    18721 cttcgggtga atgaggagcc cgttggtatc ctttacgtgg gccggagcga tctcgacgag
    18781 aacgggacga ggaccgcgct ccacgccttc aattcgctcg cgagcctctt cgcgatgaag
    18841 atggcccagt gcatggagtt cgagcgtcgg ctccgcgagc acgcgcagct ccagcgcgag
    18901 aaggatcgca tgtccgatgc catcgcgatc gcggcgcgga tccagaagaa gctcctgccc
    18961 gagcggctgc cggaggcgtc cggctacgac atcgtcgccg acctctggcc cagcctcgag
    19021 acgtcggggg atctctacga tgtgcttcgg ctctcctcga gccaggtagg gctcgtcgtc
    19081 ggggacgcga gcggcaaagg actcggggcc gcgctcctca tggccaacgt gctcgcgccg
    19141 ctccgtatga gcttcaccga gtccgcctcg ctcgtcgaga tcatggagcg attgaacgcg
    19201 cagctcgagc aatccaccga tcccgaccac ttcgtgacgc tgttcctggg ccgcctcgac
    19261 acggagcgcc acacgctcga gtacgtgaac gccgggcaca tctttcccgt gctgctcgcc
    19321 cccggcgcga aggcggtccg tctcgaagcc acgggaatac cggcgggcat gatggcgggg
    19381 acacaatacg gatcgaggac ggtggacgtg ccggccggcg cgctcctctg cctctacacc
    19441 gacggaatgc ccgaggcggc ccgcgtgggc ggccccgagt acggagagga gcggctgcac
    19501 tcgatgctgg cgcgatcgac cagccgtccg gcgagcgaca cgatggccga gcttcgaaag
    19561 gatctggacg agttcatggc cggcgagccg ctcgcggacg acacgacgat actcatggtt
    19621 cggaggacac gatgacctcg actctcgttc ggctgatgct cctcgccgcg tgcgtactcg
    19681 cgtggacatc gtgtcagcgt gaccgcagcc agacctcgga gcgccggcag gtcgagcgcc
    19741 cggacacggc cggaatctcc actccgacgc cgccggagcc cccaacgccg ccggcgaagc
    19801 aggtcatcgt cgactccccg gagagtggag cgctcatcac gagccctgtg ctggtgaccg
    19861 ggaaggctcg ggggtcctgg taccacgagg ccgaattccc gatccgcgtc ctcgactccc
    19921 aggacaagat ggtcggaacg ggcgtcgccc gtgcgaaggg cgagtggatg acctcggact
    19981 tcgtcccgtt cgaggcgtcg atcgtcttcc gcgctccgcc cgaggagtcg accggattcc
    20041 ttgtgatcga gaagagcaat ccatcgggga gccccgagca cgcggcggaa tcccgggtcc
    20101 cggttcgctt ccgctagatg gccgacacga gcgcgacccc gccccggaac cgccgcaagg
    20161 tcttggtcgt cctccccgtg tacaacgagg aggcgaagat cgggagcctc ctcgatcaca
    20221 tcgacgaggc catgcgggat gccgacatcc gctacgaggt cgtcctcgtc gacgacggga
    20281 gcaaggacgg gacggacgcg atcatccgcc ggcgcgccgc tcacatgccg ctcatgctca
    20341 tgcggcacga ggtgaacaag gggctcggcg ccacgatccg ggacggactc tttgccgcgg
    20401 ccgaagccgc gggcgaggcg gacatcatcg tcaccatgga cgcggacgac acacacgcgc
    20461 ccggcctcgt gctccgcatg gttcgaatga tctccgaggg gtacgacgtc gtcatcgcct
    20521 cgcgctaccg cgaaggatct cgatgcgtgg gcgtgccgct ccaccgtctg gccttgagcc
    20581 acggcgcgtc ctggctcttt cgcgtggtct ttcccatcca gggcgtgcgc gactacacgt
    20641 gcggctaccg cgcctatcgc gccgccgtgg tcaaggcggc cttcgcgcgc tacggggagg
    20701 agttcctgga ccagggcggg ttccagtgca tggtggacat cctgctcaag ctccggcgca
    20761 tgaatctgat cttcggagag gttccgttca tcctccgcta cgactggaag gaaggcggca
    20821 gcaagatgaa cgtgacccgc acggtcaagg acacgctggg actcatgtgg aagcggcgct
    20881 tcggcggatg agcgaggcgc gattcgagca ggagtactac gcgtccgtct atcccgacta
    20941 cgcgcgtcag aatccagccc ggaaactgcg gttctatcgc gggctggtcg agcgcgtcgc
    21001 gcccgtccgc gctccggtgc ggatcctcga catcggctgc gcgttcggcg cgtttctctc
    21061 ggcgctcgat ccgcgctggg agcgctacgg cacggacgtg agccggtacg cggtggagaa
    21121 ggccgcggcg gctctcccgg agatccactt cgcacggacg gacatcgatg cgattccctt
    21181 ccccggcccc ttcgacatga tcacgtcctt cgacgtcatc gagcacatcc cctcgctgga
    21241 cgcggtggcg tcggcggtgg agtccaggct cgcgccgggc ggacacttcg tcttcgtggt
    21301 gccggtgtac gacggcgtga ccgggccgat catccgtctc ctcgacaagg acgaaacgca
    21361 cgtgcacaag cgctcgcgcg acttctggct cgattgggcg cgcggtcggt tcgaggtggt
    21421 cgcgtggtgc gggatctacc gatatctggt tcccggattc gggtacgcgc atctccccac
    21481 gcgacggctg cgccgcttca cgccggcgat cgcggtcacg gcaaggaagc gggaccgatg
    21541 agacgcgtgg gtcgggggcc tacgcgatga gccggaggca gggacgcgct tccaagcctg
    21601 ggcggaagcc cgagcgatcc gccggacccc ggcgtccgga tcggcggcga gtctggacct
    21661 tcgtcgcgat cgcgatcatc gtgctcgtgt ctctcgcgcg ggccatccac ctcaacttcc
    21721 ccctggagcg cgacgaaggc gagttcggct acatcgccca gcagctcctc cacggtgtgc
    21781 cggtctacct gtccgcgtac acccagaagc tgcccggcgc gtactacgtg tacgcgctgt
    21841 tcctgttgct gttcgggcag agcaccgtgg gcatccatct cgggtttctt ctcgccaacg
    21901 cggctacgat ggcgctggtc ttcctcatcc tccgaaagac acacggcgga cttgccggct
    21961 gcgcgggcgc gctcgtctat ggggtgatgg cgctgtcgcc tgctctgctc ggctcctccg
    22021 gccatgccac gcacttgatt gcgttcttct cggtgttggg gctctacgcg ctgctcgagg
    22081 cccgcgaacg gggcggcctg tggctgtacc tcgtcagcgg cctctcgttc gggatggcgc
    22141 tct
//
LOCUS       DASTKC010000005        10251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1423750, whole genome shotgun sequence.
ACCESSION   DASTKC010000005 DASTKC010000000
VERSION     DASTKC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423750"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..713
                     /locus_tag="VFR25_00200"
     CDS             96..713
                     /locus_tag="VFR25_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET9939497.1"
                     /translation="MKAAVAILARAPVPGRAKTRLTPPYTPEAAAHVARACLLETLHR
                     FPRAIDVPFTLFLDGDPDPALIETIRAAGLGLIPQAEGDLGARLAAAFRALRAGSAES
                     VVAIGADSPTLDPARIAEAIAALDECDVVLGPADDGGYYLVGARGDRGGIFDGIPWST
                     GRTLEVTVERARALGYAVRLLAPHYDIDDAATLARAEAEGFTFEA"
     gene            complement(697..1152)
                     /locus_tag="VFR25_00205"
     CDS             complement(697..1152)
                     /locus_tag="VFR25_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9939498.1"
                     /translation="MPRTSAGILLFRGSGPELEVLLVHPGGPFWAKKDLWGIPKGEIH
                     LGEDPLEAARREFEEETGFAVDGQFLPLGEVRQKSGKIVRAWAVRGDLDADAIRSNEF
                     EVEWPPGSGRKRSYPEIDRGAWYGLDEAARRIRPEQRPLLDALVATLRR"
     gene            complement(1166..1978)
                     /locus_tag="VFR25_00210"
     CDS             complement(1166..1978)
                     /locus_tag="VFR25_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9939499.1"
                     /translation="MATDSGITCPRCGERLPEGFTRCDACGAFLSAPAARAPSAPRPA
                     REPRPPAKARPAPAPTAQAISGTAWIFLAAGLLCGGAIGYTLHGAVAPREEGGAPRGP
                     ADVMAGASAGQGMPQGQMPEGQMPQGQMPQEVAKMISEYRQRLATNPDDVDANIGMGN
                     LYFDSSQWERAVDSYQRALDKAPGNADVRVDMAVALHNLGQNQKAKDEMERVTRESPE
                     HRNAWLNLGVVSAQMGDRAGAVKAWEQYLKLDPNGPHSASIRAQIENMKRGS"
     gene            2032..2994
                     /gene="rsgA"
                     /locus_tag="VFR25_00215"
     CDS             2032..2994
                     /gene="rsgA"
                     /locus_tag="VFR25_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET9939500.1"
                     /translation="MPPRRTLDYTGTIVEPIRTPSSSWIVAQVHGASVELWDGHTFQV
                     AALGGQIKTSADPQHTNALAVGDRVDVEPSGSGTLRVVARHERRTQLERAANDPRSRV
                     QIVAANATRTLIVTSLRDPPFRPGLVDRWALLARRGGMEPWLCLNKVDLGTQADAERA
                     VAEAAIPLTAHFLSAQTGWGIDELRAAVAGDTSVLVGHSGVGKSSILRLLVPGAEAST
                     GDVSAKNKKGRHTTSSARLYPMPGGGVLIDTPGVRSVPLGRAEVGEVASVFAEIADAP
                     PCRFRTCTHRAEPGCSVLQGLEAGSVPRPIYARYRRLLEEAEVR"
     gene            2991..4508
                     /locus_tag="VFR25_00220"
     CDS             2991..4508
                     /locus_tag="VFR25_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9939501.1"
                     /translation="MSDTAGELRVEERLGELLIVGFPGTEWTPEIESELRRIRPAGVI
                     FFAANFPDAGTAARLCRAVHATLGSAAEPPLVCADEEGGMVSQLSGFWEVPPSARAVA
                     ASGGPPLVKDLAQRTARRLLALGVNLDFAPVLDIHSNPLNPVIGIRSFGTAASQVTAC
                     GRAAVEGMEAAGIIPVVKHFPGHGDTSLDSHVELPRLEADGRLLALRELRPFEAAVAW
                     GAPVVMVAHVVVPALDPDPGRPATLSPPVVTGTLRERMGFQGVVVTDALEMAGVTSRG
                     SYGEVAVRAIEAGSDLLLYSKLHPGPDEALVALREALRAGRLSEARILESLGRLRRLR
                     AGSAARPRDWGPGIEREARDMITHEELERVADGAFRILRQGAGGIPLRSPVDVLEINR
                     PTSRAGMTDLLRAHGMTAEERQADPATWPKTIQGSALLTVAVRGSTTPEQEALGRAWL
                     TRFPETVTVASLNPHAVDGWPEVRTLLATFDNMPATRRALARRLTVAGTLAKAQK"
     gene            complement(4569..5069)
                     /locus_tag="VFR25_00225"
     CDS             complement(4569..5069)
                     /locus_tag="VFR25_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET9939502.1"
                     /translation="MKRIATILGVAVLTLGLAATSWAGGASCSGDGAKSSSATMSSGG
                     HCSGDAEHTMASGKCEGVKANQVMYSFSVPAVECEHCVDSIQKTAMATKGIQCAHVDL
                     STRTAYVIADKKLSKQQVSKMLTEAGFKNKFTAQGKNAQAAFTKAIASGDKAVDACCA
                     KGKDKV"
     gene            complement(5149..6384)
                     /locus_tag="VFR25_00230"
     CDS             complement(5149..6384)
                     /locus_tag="VFR25_00230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9939503.1"
                     /translation="MNQHHGAGARPGRSRRTRAARTGLVLCSALFLAGLAAAAPARKP
                     APAKPAPPAAELARIRWWTAPENTRLVLDLTRPLGAEPQFRLADSLTFEIYLPKARKS
                     AAVTTEFVGDSLVADIFPAVSETGTSVRIRLKRPTTPLGFTLPASEGNPDRIVVDVPA
                     PPNPEAEKQLAQKVTELKQKRNLIVAIDAGHGGEDPGAIGYRRLQEADITLAIAKKLK
                     AQLDIMPGVTGFLTRSGDYFVPLRKRIDIARRFQADLFVSIHCNASRDRDATGTEIYF
                     LSLTGATDEAARGLAEKENAADLIGGVSPEAGDDLLSILFDLRQNDTIRRSSDLAESM
                     IDAVGGDSRLTTRGIKQAGFVVLKAPEIPSVLVETAFITNAREAAILKDGQFQNKFAE
                     MLAQGIDQYRTSHVRATSN"
     gene            complement(6392..7933)
                     /locus_tag="VFR25_00235"
     CDS             complement(6392..7933)
                     /locus_tag="VFR25_00235"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET9939504.1"
                     /translation="MESRETIRAAKALMLAGGLLIIGIVVGFILAGDLNLSPQVSAKD
                     AAPAITGALESPFTRIADRTLPAVVSIETKRTINHPSGGSFDGPYGDFFRRLFPDNPQ
                     GQGQGQGDDNDNDNDNDQTPQRQMKVPSSGSGFIIDAQGRILTNNHVVKDASDITVTL
                     SDHRKFKAKVVGTDPSTDVAVIQIHDGEDLPVVPLGDSDEIRIGDWAIAIGNALGELD
                     GTLTVGVISAKGRSNLSIVGGAPAYQQFIQTDASINFGNSGGPLLNIRGEAIGINTAI
                     NPSGEGIGFAIPINLAKNIAHQLVANGKVVRGYLGILPQELTPELADNWGMHGRKGVL
                     VGSVSGDTPASKAGFQVKDVITTFDGKDVDDVERFRLLVADTPVNKKVKVNVLRSGQP
                     RDLYVTLSERPKDEVLQKQAQPKFELGLRVESLSSETARSLDIKDKSGVVVVDVSQGS
                     AADDAGLQRGDVIKEVNDQSVKDVTDYNAAVTKARDKNPAKPIVFLIKRGDATRFLAV
                     QPDED"
     gene            complement(8010..8363)
                     /locus_tag="VFR25_00240"
     CDS             complement(8010..8363)
                     /locus_tag="VFR25_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939505.1"
                     /translation="MAGETGFWSRMSEEFRSVSDRTRASARRAVSIGVLRVDLVSLRR
                     DRSRALSDLGARALALWNGGTPHALESDAEALRLRTWIASIDEIVRVKRAELERLRAE
                     ARETKTAGEPEGSVS"
     gene            8517..9254
                     /locus_tag="VFR25_00245"
     CDS             8517..9254
                     /locus_tag="VFR25_00245"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET9939506.1"
                     /translation="MSEPRCAYFIADSHLGQGSPESNRSRERDLLAFFDKVHEEKAAL
                     YVNGDLFDFWFEYGHAIPKRFVRVLQALGDLRRRGVPVLYVGGNHDFWIGEYLARELD
                     VPFSDVPVPLTLQGRRIYLAHGDGLGPGDHGYKVLKRVLRNRVARGLFRWIHPDIGIP
                     LAAACSHTSRHHAPRPSVSDDDLRDRLAQPRYAEGYDAVVLGHFHRPVHWQGEGKDFL
                     VLGDWVNRRTVVRLAGGAFQLTEFTST"
     gene            9546..>10251
                     /locus_tag="VFR25_00250"
     CDS             9546..>10251
                     /locus_tag="VFR25_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET9939507.1"
                     /translation="MRNQGLLKVALLGLLTLALAPGYAAAAGQGQDLSEMLASSAAVH
                     ATAVSGPILQVAPLAHDYGVVDNGNSGIFTFTLSNIGDQPLHILSVNSTDPAFFGSAP
                     SIPIAPAGSDGLNLICLINDGAFHSANVTIMSDGGNVTVNVTAQGNAAPTIAPVGDKN
                     VAAFTTLSFTVVATDADDTRDDEITYSMTSNLPPGPTFNTATGEFSWSPVDTDAGSYT
                     VTFSASDGRLTASAPAI"
BASE COUNT         1468 a         3521 c         3470 g         1792 t
ORIGIN      
        1 cccggacgcg gctacctcca gcaggtcctc tttcatctac ggcgaacgtc ggggccgttc
       61 ccctcgatgg cggcgctccg ccggcgcgcg gccggatgaa ggcggcggtc gcgatcctcg
      121 ctcgagcccc cgtgccgggc cgggccaaga cgcgcctcac gccgccttac acgcccgagg
      181 cggcggcgca cgtggcgcgg gcgtgtctcc tggagacgct tcaccgcttc cctcgcgcga
      241 tcgacgtccc gttcacgctg ttcctcgatg gggaccctga tcctgcgctc atcgaaacca
      301 ttcgcgcggc gggcctcggc ctgatcccgc aagcggaggg cgatctcggc gcgcggctcg
      361 cggctgcctt ccgcgcgctt cgcgcgggca gtgccgagtc cgtggtcgcg atcggcgcgg
      421 acagcccgac gctcgatcca gcccggatcg cggaggcgat cgccgcgctc gatgaatgcg
      481 atgtggtgct gggccccgcg gacgacgggg gctattacct ggtcggcgcg cgtggggacc
      541 ggggcgggat cttcgacggg attccatgga gcacgggacg cacgctcgag gtcacggtcg
      601 agcgcgcccg ggcgctcggc tacgcggtgc gtctgctcgc gccgcactac gacatcgacg
      661 acgcggcgac gctcgctcgg gcggaagcgg aggggttcac cttcgaagcg tagcgacgag
      721 cgcgtcgagg agcgggcgct gctccggccg gatccgacgc gcggcttcgt cgagaccgta
      781 ccaggcgccg cggtcgatct ccggatacga tcgctttcgc cccgagcccg gcggccactc
      841 cacctcgaac tcgttgctcc ggatcgcgtc ggcatcgaga tcgccgcgca cggcccacgc
      901 gcgcacgatc tttccgctct tctggcgcac ctcgccgagc ggcaggaact gaccgtcgac
      961 cgcgaagcct gtctcttcct cgaattcgcg ccgcgccgct tcgagcggat cttcgcctaa
     1021 gtggatctcg cctttgggaa tgccccagag atccttcttg gcccagaacg gtccgccggg
     1081 atgaaccagg agcacttcga gctccggacc gcttccgcga aagagaagga tcccggcgct
     1141 cgtcctgggc acggcgtgag cggggctagg agccccgctt catgttttcg atctgtgcgc
     1201 ggatcgacgc ggagtggggt ccgttggggt cgagcttcag gtactgctcc caggccttga
     1261 cggcgccggc gcggtcgccc atctgcgcgc tcacgacgcc gaggttgagc catgcgttgc
     1321 gatgctcggg gctctcgcgc gtgactcgct ccatttcgtc cttcgccttc tggttctggc
     1381 cgagattgtg gagcgcgacc gccatgtcca cgcgcacgtc ggcgttcccg ggggccttgt
     1441 cgagcgcccg ctggtagctg tcgaccgccc gctcccactg gctcgagtcg aagtagagat
     1501 tccccatgcc gatgttcgcg tcgacgtcgt cgggattcgt cgccagcctc tggcgatact
     1561 ccgagatcat cttcgcgacc tcttgcggca tctgcccctg aggcatctgc ccctccggca
     1621 tctgaccttg cggcattccc tgacccgcgg aagcgccggc catcacgtcg gccggccccc
     1681 tgggcgcgcc cccttcctcg cgcggcgcca cggcgccgtg gagcgtgtag ccgatcgccc
     1741 cgccgcagag gagtcccgcg gctaagaaga tccacgcggt gccggagatg gcctgcgccg
     1801 tgggcgcggg tgcggggcgc gctttcgcag gcggcctcgg ctcgcgcgcg ggacgcggcg
     1861 cgctcggcgc tcgggcggcg ggcgccgaca ggaacgcgcc gcaggcatcg catcgggtga
     1921 atccctcggg gagccgctcg ccgcagcgcg ggcaggtgat gccggagtcc gtagccatgc
     1981 tcgcagtgta ctaatttcgc agcgaatggg ccactcgagg gaacggttgc cttgccgccc
     2041 cgacgcacgc tcgactacac tgggacgatc gtcgagccaa tccgcacccc ctcatcgagc
     2101 tggatcgtcg cgcaggtgca cggcgcgagc gtcgagcttt gggacggcca caccttccag
     2161 gttgccgctc tcggcggaca gatcaaaacg tcggccgacc cccagcacac gaacgcgctc
     2221 gcggtcggcg atcgcgtgga cgtcgagccg agcggatcgg gaacgctccg cgtcgtcgcg
     2281 cggcacgagc ggcggacgca gctcgagcgc gccgcgaacg atccccggag ccgcgtccag
     2341 atcgtggcgg ccaacgcgac gcgcacgctc atcgtcacct cgctccgcga tcctccgttc
     2401 cgccccggcc tcgtcgatcg ctgggcgctc ctcgcgcgcc gcggcggaat ggagccgtgg
     2461 ctctgcctca acaaggtgga tctcgggacc caggccgacg ccgagcgcgc ggtggcggag
     2521 gccgcgatcc cgctcacggc ccactttttg agcgcgcaaa ccgggtgggg catcgatgag
     2581 ctccgggccg cggtcgccgg ggacacgagc gtgctggtcg gccactcggg tgtgggcaag
     2641 tcctcgatcc tccgcctcct cgtgccggga gccgaagcct cgacaggcga cgtgagcgcg
     2701 aagaacaaga agggacgcca taccacgtcg agcgcgcggc tctatccgat gccgggaggg
     2761 ggggtgctca tcgacacgcc cggagtgcgc agcgtcccgc tcgggcgggc cgaggtcggc
     2821 gaggtcgcgt ccgtattcgc tgagatcgcc gacgcgcccc cgtgccggtt ccggacgtgt
     2881 acgcatcgcg ccgagcccgg ctgctcggtg ctccaggggc tcgaggcggg ctccgtcccg
     2941 cgcccgatct acgcgcgcta ccggagactc ctcgaggaag cggaggttcg atgagcgaca
     3001 cggcgggtga gcttcgggtg gaggagcgcc tcggcgagct cttgatcgtc ggcttccccg
     3061 gcacggagtg gacgcccgag atcgagtccg agctccggcg catccgcccc gccggggtga
     3121 tcttcttcgc ggccaacttc cccgacgcgg gaacggcggc gcggctttgc cgcgccgtgc
     3181 acgcgaccct gggatccgcg gccgagccgc cgctcgtgtg cgcggacgag gaagggggca
     3241 tggtctccca gctctccgga ttctgggagg tgcctccgtc ggcgcgtgcg gtggccgcct
     3301 cgggaggacc gccgctcgtc aaggatctgg ctcagcgcac ggcgcgccgg ctcctcgcgc
     3361 tcggcgtgaa tctcgatttc gcgccggttc tcgacatcca ctccaatccg ttgaacccgg
     3421 tgatcgggat ccgctcgttc ggcacggcgg cgtcgcaagt gaccgcgtgc ggacgcgccg
     3481 cggtcgaggg aatggaagcg gcggggatca tcccggtcgt gaagcatttc cccggacatg
     3541 gcgacacgtc gctcgactcc cacgtggaac ttccgcgctt ggaggcagac ggtcggctcc
     3601 tcgcgctccg cgagctccgc ccgttcgagg ccgcggtcgc gtggggcgcg ccggtcgtga
     3661 tggtcgcgca cgtggtggtg cccgcgctcg atcccgaccc cggtcgcccc gccacgctct
     3721 cgccgccggt cgtcaccgga acgctccgcg agcgcatggg attccagggc gtggtcgtga
     3781 ccgacgcgct cgagatggcg ggcgtgacct cgcgcggatc ctacggcgag gtcgccgtcc
     3841 gcgcgatcga ggccggctcg gatctcctcc tctattccaa gctccatccc gggccggacg
     3901 aggcgctcgt cgcgctccgc gaggcgcttc gcgcgggacg gctcagtgaa gcgcgcatcc
     3961 tcgaatcgct cggacggctc cgccggctcc gggccgggag cgccgcgcgg ccgcgcgact
     4021 gggggcctgg catcgagcgg gaagccaggg acatgatcac ccacgaagag ctggagcgcg
     4081 tcgccgacgg cgcgttccgc atccttcgcc agggagcggg cggcattccg ctgcgctcac
     4141 ccgtggacgt gctcgagatc aaccgcccca cctcgcgagc cgggatgacg gacctcctcc
     4201 gcgcgcacgg catgaccgcc gaggagcgcc aggccgatcc ggcgacgtgg cccaagacaa
     4261 tccaggggag cgcgctcctc acggtcgcgg tgcgcggctc cacgacgccc gagcaggaag
     4321 cgctggggcg cgcgtggctc acgcgattcc ccgagacggt cacggtggcg tcgctcaacc
     4381 cgcacgccgt ggacggatgg cccgaggtcc ggacgctcct cgccaccttc gacaacatgc
     4441 ccgccacgcg gcgcgccctc gcgcgccgtc tcaccgtcgc cgggacgctg gccaaagccc
     4501 agaaataaaa acggcccccc gtccgcgagg acggagggcc gcgatatcgc gatgcgtgaa
     4561 ggtggggctt agaccttgtc cttgcccttg gcgcagcacg cgtccacagc cttgtcgccg
     4621 gaggcgatcg ccttcgtgaa cgccgcctgg gcgttcttgc cctgggccgt gaacttgttc
     4681 ttgaagcccg cctcggtgag catcttcgag acctgctgct tgctcagctt cttgtccgcg
     4741 atcacgtagg cggtgcgggt ggagaggtcg acgtgggcgc actggatgcc cttcgtcgcc
     4801 atcgcggtct tctggatgct gtcgacgcag tgctcgcact cgaccgcggg caccgagaag
     4861 ctgtacatga cctggttggc cttgacgccc tcgcacttgc ccgaggccat cgtgtgctcg
     4921 gcgtcgcccg agcagtgacc gcccgaggac atcgtcgccg aggacgactt ggcgccgtcg
     4981 cccgaacagg aagctccgcc ggcccacgaa gttgcggcca ggccgagggt gagaaccgcg
     5041 actccaagaa tggtggcgat ccgtttcatg gctttctcct agcgtgggtt ggcgtagagc
     5101 ctatccggct ccggtaagga taccccgggt cccggggcgc gtctcctgtc aattcgacgt
     5161 ggcccggacg tgcgaggtcc ggtactggtc gatgccctgg gccagcatct ccgcgaactt
     5221 gttctggaac tggccgtcct tgaggatggc cgcctcccgg gcgttggtga tgaaggcggt
     5281 ctcgaccagg accgacggga tctccggggc cttcaggaca acgaagccgg cctgcttgat
     5341 gccgcgggtc gtgagcctcg agtccccgcc gacggcgtcg atcatcgatt cagcgaggtc
     5401 gctgctcctg cggatcgtgt cgttctggcg caggtcgaag aggatcgaga ggaggtcgtc
     5461 tccggcctcg ggggagacgc cgccgatcag gtccgccgcg ttctccttct cggccagacc
     5521 ccgcgccgcc tcgtccgtcg cccccgtcag ggagaggaag tagatctcgg tccccgtcgc
     5581 gtcccggtcc cggctcgcgt tgcagtggat gctcacgaag aggtccgcct ggaagcgccg
     5641 ggcgatgtcg atccgcttcc gcagcggaac gaagtagtct cccgaccggg tcaggaaccc
     5701 ggtgaccccg ggcatgatgt ccagctgagc cttcagcttc ttcgcgattg cgagcgtgat
     5761 gtccgcttcc tgcaacctcc ggtaaccgat cgcgcccgga tcttccccgc cgtgtcccgc
     5821 gtcgatggcc acgatcaggt tccgcttctg cttgagctcg gtgaccttct gcgcgagctg
     5881 cttctcggcc tcgggattgg gtggcgcggg aacgtccacg acgatgcggt cgggattccc
     5941 ttcgctcgcg gggagcgtga atcccagcgg cgtggtcggt cgcttcaggc gaatccggac
     6001 ggacgttccc gtctcggaca ccgcggggaa gatgtcggcc acgaggctgt cccccacgaa
     6061 ctcggtcgtg accgcggccg acttgcgtgc cttggggagg tagatctcga acgtgagcga
     6121 gtccgcgagc ctaaactgcg gctccgcgcc gagcggcctc gtgagatcga ggacgagccg
     6181 cgtgttctcg ggagccgtcc accagcgaat gcgcgcgagc tcagcggccg gcggcgcggg
     6241 cttggccggg gcgggtttcc tcgccggcgc ggccgcggcg agccccgcaa ggaagagcgc
     6301 cgagcatagg acgaggcccg tccgagccgc cctggttcgg cggcttcgtc cgggcctcgc
     6361 gcccgcgccg tgatgctggt tcaaatcccg ctcaatcctc gtccggctgc accgcgagga
     6421 atcgcgtggc gtcgccgcgc ttgatgagga aaacgatcgg cttggccgga ttcttgtcgc
     6481 gcgccttggt cacggcggca ttgtagtcgg tgacgtcttt gacggactgg tcgttcactt
     6541 ccttgatcac gtcgccgcgc tggagtccgg cgtcgtcggc cgcgctcccc tggctcacgt
     6601 cgaccaccac gacgcccgac ttgtccttga tgtcgaggct gcgcgccgtc tcgctcgaga
     6661 gggactcgac ccggagcccg agctcgaact tgggctgcgc ctgcttctgg agcacctcgt
     6721 ccttcggacg ctcgctcaag gtcacgtaga ggtcacgagg ctgcccggac cggagcacgt
     6781 tgaccttgac cttcttgttc accggcgtgt ccgcgacgag gagacggaag cgctccacgt
     6841 cgtcgacgtc cttgccatcg aaggtcgtga tgacgtcctt cacctggaag cccgccttgg
     6901 acgccggcgt gtcgcccgag acgctgccca cgagaacgcc cttccggccg tgcatgcccc
     6961 agttgtcggc cagctcgggg gtcaactctt gtggaaggat gccgaggtag ccgcgcacca
     7021 ccttgccgtt ggccacgagc tggtgagcga tgttcttcgc gagattgatc ggaatggcga
     7081 agccgatgcc ctcaccggat ggattgatcg cggtgttgat gccgatcgcc tcgccgcgga
     7141 tgttgaggag cggcccgccg ctgttgccga aattgatcga cgcgtcggtc tggatgaact
     7201 gctggtaggc cggggcgccg cccacgatgg agaggttgct ccgtcccttg gcgctgatca
     7261 cgccgacggt cagcgttccg tccagctcgc cgagggcgtt gccgatcgcg atggcccagt
     7321 cgccgatccg gatctcatcc gagtcgccga gcggcacgac cggaaggtcc tcgccgtcgt
     7381 ggatctggat caccgcgacg tccgtgctcg gatccgtccc gacgaccttc gccttgaact
     7441 tccggtggtc gctcagcgtc accgtgatgt cggacgcgtc cttcacgacg tggttgttcg
     7501 tgaggatccg tccctgcgcg tcgatgatga accccgagcc gctgctcggc accttcatct
     7561 ggcgctgcgg cgtctggtcg ttgtcgttgt cattgtcgtt gtcgtcgccc tgcccctggc
     7621 cctgtccctg cggattgtcc gggaagaggc ggcggaagaa gtccccgtac gggccgtcga
     7681 agctgcctcc gctcggatgg ttgatggtgc gcttcgtctc gatggagaca accgccggca
     7741 gcgtgcgatc ggcgatccgg gtgaaggggc tctcgagcgc gcctgtgatc gccggagccg
     7801 cgtccttggc gctcacctgc ggcgatagat tcaaatcgcc agccaggatg aacccgacca
     7861 cgatgccgat gatcaacagt ccgccggcga gcatcagggc tttcgccgcg cgaatcgttt
     7921 ctctggactc catgttcgct acctccgttg aactgatcgg gagccctcga ggctcgccga
     7981 aactccgccc ggcgcagggg acgccggagc tacgacaccg atccttccgg ctccccggcc
     8041 gttttggtct ctcgtgcttc cgcgcggagc cgctcgagct ccgcacgctt cacgcgcacg
     8101 atctcatcga tcgaggcgat ccaggtgcga aggcggagcg cctcggcgtc cgattcgagt
     8161 gcgtgaggcg tgccgccgtt ccagagcgcg agcgctcgcg cgccgagatc ggacagagcc
     8221 cgagagcggt cgcgccggag cgatacgagg tcgacccgga gcacgccgat cgacaccgcc
     8281 cgacgcgcgc tcgcgcgcgt ccgatcggag accgatcgga actcttcgga catgcggctc
     8341 cagaacccgg tttcgccagc cataaatcct cacagcacag gtagttggta caatctagga
     8401 atcgccggtg aggcctgtca agctggtatt acccgttcgg gtatcgggag ttccgctggc
     8461 gggagcctcg gccctccgtc ttgcgagagg cccggcgatg tctatactga cgcctcatgt
     8521 ccgagcccag atgcgcctat ttcatcgccg attcgcatct cggacaaggg tctccggaat
     8581 cgaaccgctc ccgcgagcgg gatctcctcg ccttcttcga caaggtccac gaagaaaagg
     8641 ctgcgctcta tgtgaacggg gacctcttcg atttctggtt cgaatacggc catgcgatcc
     8701 cgaagcgttt cgtccgggtg ctccaggccc tgggcgacct gcggcgtcgc ggcgtgcccg
     8761 ttctctatgt cggcggaaat cacgacttct ggatcggcga gtacctggcg cgcgagctcg
     8821 acgtcccctt ctcggacgtg ccggtccctc tgacgctcca gggacggcgg atctaccttg
     8881 cccacgggga cgggctcggt ccgggcgacc acggttacaa agtcctgaag cgggtgctcc
     8941 gaaaccgcgt cgcgcgcggg ctctttcgct ggatccatcc ggacatcggc attcccctgg
     9001 ccgcagcctg ctcccacacg agccgccacc acgctccgcg cccctcggtc agcgacgacg
     9061 acctccgcga ccggctcgcc cagccgcggt atgcggaagg ctacgatgcg gtcgtcctcg
     9121 gtcatttcca ccgtccagtc cattggcagg gggagggtaa ggacttttta gtgctggggg
     9181 actgggtcaa ccggcgcacc gtcgtccgcc tggcgggcgg agccttccaa ctcacggaat
     9241 ttacgagtac ttagccgctt cgggaccgcg ccgccggggg actccaaggg ggtgcgggtt
     9301 tcgcttgact caagtgcccg tttgtattac acttagaagg ttctagcacc accctgtcca
     9361 caaattggtt gttgccgcag ggatgcgtgc gcgtcaagcg cgcgtgtcgg tgcggctctc
     9421 tgtttcgttg agtgatctgg aacctaatta ccgagacgag gggaggtgag catccggtcg
     9481 gctctgtttg gttgaaagcc tactagctgc ttcgttgctc tgatttccat gagggaggac
     9541 acgcaatgcg caaccaaggt cttctgaaag tcgctctcct cgggcttttg accctcgctc
     9601 tcgcccccgg ctacgccgcg gcggccggac agggacagga cttgtcggag atgctcgctt
     9661 cgagcgccgc ggtgcacgcg accgcggtga gcggcccgat tcttcaagta gccccgctgg
     9721 cgcacgacta tggcgtcgtg gacaacggga actccggcat tttcacgttc acgctgtcga
     9781 acatcggtga ccagcctctg cacatcctca gtgtcaactc gaccgatccg gcgtttttcg
     9841 gctcggcgcc gagtatcccc atcgctccgg ctggttcgga tgggctgaat ctcatctgtt
     9901 tgatcaacga cggcgcgttc cacagcgcga atgtcacgat catgtccgat ggcggaaatg
     9961 tgacggtgaa cgtcacggcc cagggcaacg cggctccgac gatcgctcct gtgggcgaca
    10021 agaacgtggc ggcattcacg acgctttcgt tcacggtcgt ggcgacggat gcggacgaca
    10081 cgcgggacga cgagatcacc tactcgatga cctcgaatct gcctcccggt ccgacgttca
    10141 acacggcgac gggcgagttc tcgtggagcc cggtggatac cgatgccggc agctacacgg
    10201 tgacgttctc ggcgagcgac ggtcgtctca cggcgtcggc tcctgcgatc a
//
LOCUS       DASTKC010000006         7119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1898031, whole genome shotgun sequence.
ACCESSION   DASTKC010000006 DASTKC010000000
VERSION     DASTKC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898031"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFR25_00255"
     CDS             <1..651
                     /locus_tag="VFR25_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9939508.1"
                     /translation="TISVLLNSGSGTFLPATAYVKPGTWSGIAVGSADQDASPDLFVV
                     DGYNYSVSILNGSPGGGFTFQGRSWGVPATPEGLAVGDLNEDGRTDIVASGSALYGAA
                     GFAQVLLYEGGTVTGVESSPVIDRNGLAQNRPNPFNPRTEISFEIARSGPATLRVYDA
                     SGRLVRTLLERSLDAGLHSVGWDGMDAEGRKAASGVYFYRLVTPGFDSAKKMLLMK"
     gene            873..1745
                     /locus_tag="VFR25_00260"
     CDS             873..1745
                     /locus_tag="VFR25_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9939509.1"
                     /translation="MIHERRVAALSGAIGLTITLLLGGLAVYLFVGAVQAGRPLLLAT
                     TVLPLAVVTALCAAGLFTVQPNQAVVLILFGSYTGTVRDSGWWWTNPFNTRKKISLRV
                     RSLNGHMIKVNDHAGNPVEIAAVVVWNVQDTAQAVFDVENYEDFVSVQSETAVRHLAS
                     IYPYDEYDHDAISLRGSTDKVSEALRAELQERLRPAGVQIVEARLSHLAYAPEIAGAM
                     LQRQQAAAIIAARQRIVDGAVGMVEMALARLSANNIVELDEERKAAMVSNLLVVLCGE
                     RAATPVLNTGTLHN"
     gene            complement(1906..2739)
                     /locus_tag="VFR25_00265"
     CDS             complement(1906..2739)
                     /locus_tag="VFR25_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939510.1"
                     /translation="MRTDTVWTKTYERPWIVAAIAAGTVATLFLLFAPRAEAQYGGEI
                     RGDRQELHHDQWDRNGDWTDVRLFDRQLQRLVRAERVGDRFEERRARAELQRLMRLEL
                     MESRRDLRLDQGEARWDRDDTGARWDDRRDARATRARLARERRVVHELASIETNVSRG
                     APWALDRERRLLREFSRLTREDALASGRELREDRRELWNDLRDDRDEGRRDFGGDGDE
                     RSFFKNDRPAPPPPPQNWNRDQDDREGVDGRSDQNAPPDLDRQDEDRTPPPPPDDEDA
                     L"
     gene            complement(2816..4258)
                     /locus_tag="VFR25_00270"
     CDS             complement(2816..4258)
                     /locus_tag="VFR25_00270"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9939511.1"
                     /translation="MTTGFRMRAPVTALCAALALTALLGAPASAEIVLPKSRAITLSN
                     GAQIILAERHDLPLIAFRAFVNGGSLADPPGKEGLAGLTGAMLRKGAGKRSALQISQA
                     ADGAGASFDTGGGLEIFWAAGEFMSKDEALMVELLRDTFRHPLFPDSEFVKLRAQTVD
                     ALKSEKDDPWGVLGQYGLAYLFPDHPYGRPSSGDESTVAGITRDDVIACYRGRFGGDR
                     LILAVVGDFDSKRMESRLKAAFGDWARATEPLAKIPETPRVDGRRVLLVDKPDASQTY
                     FWMGNRGIGRHDPDRDVVDVANTAFGGRYTSILNSEMRTKSGLTYGVRCRFLRLGQPA
                     SVAISSFTKTDSTQRAMDMALRLLGQLREKGIDATTVASVKSYLSGLYPPDYETGDQI
                     AAAFADLAYHGFPITEVTQYVERIQRVPDASILPVIKRVYPDPKDLSIVMIGNAAAIR
                     KVAQGYGPVREIRFDEPLLRTIHEASARAR"
     gene            complement(4255..5682)
                     /locus_tag="VFR25_00275"
     CDS             complement(4255..5682)
                     /locus_tag="VFR25_00275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9939512.1"
                     /translation="MAQRSLETRLRLPAALVAAFLALAPPATAQPRETTKSTSKKSAI
                     GSDVVAKTLPNGLRVIVWPDHDIPNVALYIWHKVGSRNEHNGITGLSHFFEHMMFLGS
                     EHYPGGEFDRVMEGNGGANNAFTTQDVTVYSDWFAKSALDLVLRMEADRMCCLTFDST
                     SVQSEREVVYSERRTSVDNNNQQFLDESVEATAFEAHPYQIPTIGWPSDIERWTMADL
                     KEYFRIYYAPNNAVLVVVGDVTGSEIMALMDKYFAGIPAQPPPPQVRTIEPPQLGERR
                     VEIRRPAPVPLLEVGYHAGSASGPDAEAEELLDMILTAGESSRLYQRMVDRDRVAVDV
                     DSWLQRGFDPGMYAFFVTVAPDKGLKAAEASLFDELARVAKEGVTEAELQKAKTIRLA
                     SYWRSLKTIDDKASALGRFETLRGDYRMLFTAPARYEKVTRQKVQEVARRIFTEKNRT
                     VGTLVPENEPEADAKGSSSARRGKP"
     gene            5852..6652
                     /locus_tag="VFR25_00280"
     CDS             5852..6652
                     /locus_tag="VFR25_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET9939513.1"
                     /translation="MPTMHEIDYEILGDDMQFVRIILDPGEAVVAEAGGMMYIEDGIQ
                     MDTIFGDGSQPNQDFLGKLVGAGKRLLTGESLFMTVYHNEGRERRAVAFAAPYPGKIE
                     PLHLKELGGEIIAQKDSFLAAAKGVSVGIAFNKRFGAGLFGGEGFIMERLTGDGWCFV
                     HAGGTLEKRQLAAGETLRVDTGCLVALQPSVSYDIQYVGKLKSALFGGEGLFFATLRG
                     PGTVWLQSLPFSRLASRIYAAAPQAGGRRREEGSLLGGLGNLLDGDGQ"
     gene            complement(6685..6903)
                     /locus_tag="VFR25_00285"
     CDS             complement(6685..6903)
                     /locus_tag="VFR25_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET9939514.1"
                     /translation="MNESWGRVLLVLGAALFLVGAVLTWGPRMPWLGRLPGDFRFGGS
                     NWKVYIPLGTSLLLSLVLSLLFWALRRR"
     gene            complement(6919..>7119)
                     /locus_tag="VFR25_00290"
     CDS             complement(6919..>7119)
                     /locus_tag="VFR25_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939515.1"
                     /translation="DITVSFPPGVGAQVSVESGSGGIRSELALETQVRKHDALVGRIG
                     DGRGTIGIETGSGTITLRERKP"
BASE COUNT         1153 a         2360 c         2406 g         1200 t
ORIGIN      
        1 acgatttccg tcctcctgaa ttccgggagc ggaacgtttc tccccgcgac cgcgtacgtc
       61 aaacctggta cctggagcgg tatcgcagtc gggagcgcgg accaggacgc ctcgcccgat
      121 ctcttcgtcg tcgacggtta caactattcc gtctcgatcc tgaacggctc gcccggaggc
      181 ggattcacgt tccagggccg ctcctggggc gtcccggcga cgccggaggg gctcgccgtt
      241 ggcgatctga acgaggacgg ccgcacggat atcgtggcct ccggaagcgc actctacgga
      301 gcagccggtt tcgcgcaggt tctcctgtat gagggaggga ccgtgacggg ggttgagtcc
      361 tccccggtca tcgatcggaa cgggctcgcc cagaaccggc ccaatccatt caatcctcgc
      421 accgagattt ccttcgagat tgcgcgaagc ggtccggcga cgcttcgcgt ctacgacgca
      481 agcggccgtc tggtccggac gctcctcgag cggtcgctcg atgctggact tcacagtgtc
      541 ggatgggacg gcatggacgc agagggccgg aaggcggctt cgggagtgta cttctatcgt
      601 ctggtcacgc cgggattcga cagcgcgaag aagatgctgc tgatgaagta gctaaggcgc
      661 ccggcagcag ccgggcggaa ctcctgttct ttccacgcag aggatccaag cacgaaggcg
      721 ggacccggag caatccaggt cccgccttct agttacggat tccagcctcg cacccttgac
      781 atcgcgctat ctttatgata tcatacttat atcgcaccac cggggagccc acggggggcc
      841 tgcccgaacc tttgaaagga gcgtgttcgt ccatgatcca cgaacgtcgg gtcgcggccc
      901 tgagcggggc catcggtctg accatcaccc tgcttctcgg gggattggcc gtctatctct
      961 tcgtcggcgc ggtccaggcc ggccgtcctc ttctgctcgc gacgaccgtg cttcccctgg
     1021 ctgtagtcac ggcgctttgc gcggcgggac tcttcacggt ccagccgaac caggccgtgg
     1081 tcttgatcct gttcggatcc tacacgggga cggtgcgcga cagcggctgg tggtggacga
     1141 accccttcaa cacgcggaag aagatctcgc tccgcgtgcg ttcgctgaac ggccacatga
     1201 tcaaggtgaa cgatcatgcg ggcaatccgg tcgagatcgc cgcggtcgtc gtgtggaacg
     1261 tgcaggacac ggcgcaggcc gtgttcgacg tggagaacta cgaggacttc gtctcggtgc
     1321 agagcgagac cgcggtgagg catctcgcga gcatctatcc gtacgacgag tacgaccacg
     1381 acgcgatctc gctgcgtggt agcaccgaca aggtgtccga ggcgctgcgt gccgagctcc
     1441 aggagcgtct gcgcccggcg ggcgtgcaga tcgtcgaggc caggctgagc cacctcgcgt
     1501 acgcgcccga gatcgcgggg gcgatgctcc agcgtcagca ggccgcggcc atcatcgcgg
     1561 ctcggcagcg gatcgtggat ggagccgtgg gaatggtgga gatggcgctc gcgcggctga
     1621 gcgcgaacaa catcgtggag ctggacgagg agcggaaggc tgcgatggtg tcgaacctgc
     1681 tcgtggtgct ctgcggcgag cgcgcggcga ccccggttct caatacggga acgctccaca
     1741 actgatccat ggcgccccgc aaggcattcc tgctccggat cgatccggag ctctggcggg
     1801 cgatcgaagc ctgggcggcc gacgagctac gcagcgtgaa cgggcagatc gagtacctgc
     1861 tctccaacgc tgtgaaggac gccggccgtt ccaggtcttc gaagactaga gagcgtcctc
     1921 gtcgtcagga ggcgggggcg gagtccggtc ctcgtcctgg cggtccagat caggaggagc
     1981 gttctggtca gagcggccat caacgccctc gcggtcgtcc tgatcgcgat tccagttctg
     2041 aggcggaggg ggaggagccg gacgatcgtt cttgaagaag gagcgttcat caccatcgcc
     2101 accgaaatcg cggcgtcctt catcccgatc gtccctgagg tcgttccaga gctcgcgcct
     2161 gtcctcacgt agctccctcc cgctcgccag cgcgtcctcc ctcgtcagcc tcgagaattc
     2221 ccgcagcaat ctccgctcgc gatcgagcgc ccacggcgcc ccgcgcgaga cgttggtctc
     2281 gatcgaagcg agctcgtgca ccacgcgccg ctcgcgcgcg aggcgcgcgc gtgtggccct
     2341 ggcatcgcgg cggtcgtccc agcgcgcgcc cgtatcgtcg cggtcccacc gagcctcgcc
     2401 ctgatcgagc ctgagatccc ggcgcgactc catgagctcg aggcgcatga ggcgctgcag
     2461 ctcggcccgc gcgcgccgct cttcgaaacg atcccccacc cgctccgccc gcacgagcct
     2521 ctggagctgt cgatcgaaca ggcgaacgtc ggtccagtcg ccgtttcgat cccactggtc
     2581 gtggtgcagc tcctgccggt caccccggat ctcccctccg tactgggcct cggccctagg
     2641 ggcgaagagc aagaacaggg tcgcgacggt ccccgccgcg atggccgcca cgatccatgg
     2701 gcgttcgtag gttttcgtcc acactgtgtc ggtacgcatc gcgtttcctc cggctctggc
     2761 gttggggcac aacgagttcg gtccgcgagc ggaccgatgc cggaggagag ggcaactacc
     2821 gtgcccgggc ggaggcctcg tggatggtgc ggaggagggg ctcgtcgaac cggatctcgc
     2881 gcacgggccc gtagccctgc gcgaccttcc ggatggcggc cgcgttcccg atcatcacga
     2941 tggagaggtc cttgggatcg ggatacacgc gcttgatgac ggggaggatc gacgcgtcgg
     3001 gaacgcgctg gatccgctcg acgtactggg tcacctcggt gatcggaaat ccgtgatacg
     3061 cgagatcggc gaacgcggcc gcgatctggt ctccggtctc gtagtcgggc ggatagaggc
     3121 cgctcaggta gctcttcacc gatgcgaccg tcgtcgcgtc gatccccttc tcacggagct
     3181 ggccgaggag gcgcagcgcc atgtccatcg cgcgctgcgt ggagtccgtc ttcgtgaagc
     3241 tcgagatcgc gaccgaggcg ggctggccca gccgcaggaa gcggcagcgc acgccatacg
     3301 tgagcccact cttggtgcgc atctcactgt tgaggatgga tgtgtatcgc ccgccgaagg
     3361 ccgtgttcgc cacgtccacc acgtcccggt ccggatcgtg acgtccgatg ccgcggtttc
     3421 ccatccagaa ataggtctga gacgcgtccg gcttgtccac gaggagcacg cggcgtccat
     3481 cgactcgcgg agtctcgggg attttcgcaa gcggctcggt ggcacgcgcc cagtcgccga
     3541 acgccgcctt gagccgcgac tccatgcgct tggagtcgaa gtcgcccacc accgcgagga
     3601 tcaaacgatc gccgccaaat cgaccccgat agcaggcgat cacgtcgtcg cgcgtgatgc
     3661 cggccaccgt gctctcgtct ccactgctcg gtcgcccgta gggatgatcc gggaacagat
     3721 aggcgaggcc gtactggccg agcacccccc acggatcgtc cttctcggac ttgagcgcgt
     3781 cgaccgtctg cgcgcggagc ttcacgaact cggagtccgg gaagagcggg tgccggaagg
     3841 tatcgcgcaa gagctcgacc atgagcgctt cgtccttcga catgaactcg cccgcggccc
     3901 agaagatctc gagaccgccc cccgtgtcga acgaagcgcc cgctccgtcc gcggcctggg
     3961 agatctgcag cgcggagcgc ttccccgctc ccttgcgcag catcgccccg gtgagcccgg
     4021 ccaggccctc cttgcccggg ggatcggcga gcgatccacc gttcacgaac gcccggaagg
     4081 cgatgagcgg gagatcgtgc cgctccgcca ggatgatctg cgcgccattc gagagcgtga
     4141 tcgcgcgcga cttggggagg acgatctccg cgctggcggg agcgccgagc agagccgtga
     4201 gggcgagcgc ggcgcagagc gcggtcacgg gagcgcgcat ccggaaaccg gtcgtcatgg
     4261 cttccccctc cgcgcggacg acgagccctt ggcgtcggcc tcgggctcgt tctccgggac
     4321 cagcgtcccc accgtgcgat tcttctccgt gaagatgcgt cgcgcgacct cctggacctt
     4381 ctggcgcgtc accttctcgt agcgcgccgg cgcggtgaag agcatccggt agtcgccgcg
     4441 cagcgtctca aagcgcccga gtgcggacgc cttgtcgtcg atggtcttga gcgagcgcca
     4501 gtacgaggcc aggcggatgg tcttggcctt ctggagctcg gcctcggtga cgccttcctt
     4561 cgcgacgcgc gcgagctcgt cgaacaggga cgcttcggcg gccttgagcc ccttgtccgg
     4621 cgcgaccgtc acgaagaacg cgtacatgcc gggatcgaag ccccgctgga gccaggagtc
     4681 cacgtccacg gccacgcgat cgcgatcgac cattcgctga tagagccgcg acgattcgcc
     4741 ggccgtgagg atcatgtcga ggagctcctc cgcctcggcg tcggggccgc tcgccgagcc
     4801 cgcgtggtat ccgacctcga ggagcggcac cggggcggga cggcggatct cgacccggcg
     4861 ctcacccagc tgcggaggct cgatcgtgcg gacctggggc ggcggcggct gcgccgggat
     4921 gcccgcgaag tacttgtcca tgagcgccat gatctcggac cccgtcacgt ctccgacgac
     4981 gacgagcacc gcgttgttcg gcgcgtagta gatgcggaag tactccttca ggtcggccat
     5041 ggtccagcgc tcgatgtcgc tcggccaccc gatcgtgggg atctggtagg ggtgcgcttc
     5101 gaacgccgtc gcttccaccg actcgtcgag gaactgctgg ttgttgttgt ccacgctcgt
     5161 gcgccgctcg gagtagacga cctcgcgctc gctctgcacg gaggtggagt cgaacgtgag
     5221 gcagcacatc cggtccgcct ccatgcggag gacgagatcg agggcgctct tcgcgaacca
     5281 gtcggaatag acggtcacgt cctgggtcgt gaaggcgttg ttcgcgccgc cgttcccctc
     5341 catgacccgg tcgaattcgc cgcctgggta gtgctccgag ccgaggaaca tcatgtgctc
     5401 gaagaagtgc gagagccctg tgatgccgtt gtgctcgttg cggctcccca ccttgtgcca
     5461 gatgtagagc gcgacgttcg ggatgtcgtg gtcgggccag acgatcacgc gaagaccgtt
     5521 gggcagggtc ttggcgacga cgtcggagcc gatcgcgctc ttcttggaag tggactttgt
     5581 ggtctcgcgc ggctgcgcgg tcgccggtgg ggcgagcgcc aagaaggccg cgacgagcgc
     5641 ggccggcagc cgtaacctgg tctccagact gcgttgggcc attcttcgaa acacggcgtc
     5701 cgggtcctcc gtaggatcga cgagtctagt cccggcccgg gtttctgtca cgcggcgccc
     5761 gtccggccct atagtgaccg gcaatcgaag gcttccccgg acggcaagat cgccgaccgt
     5821 gaaggctacg acactccaga ggaggagctc catgccgacc atgcacgaga tcgattacga
     5881 gatcctgggc gacgacatgc agttcgtccg gatcatcctg gatccgggcg aagcggtcgt
     5941 cgccgaggcg ggcggaatga tgtacatcga ggacgggatc cagatggaca ccatcttcgg
     6001 cgacggctcg cagccgaacc aggatttcct cggcaagctg gtcggcgccg gcaagcggct
     6061 cctcacgggc gagtcgctct tcatgaccgt ctaccacaac gagggacgcg agcgccgcgc
     6121 ggtcgcgttc gccgctccgt accccggcaa gatcgagcct ctccacttga aggagctcgg
     6181 gggcgagatc atcgcgcaga aggactcgtt cctcgcagcg gcgaagggcg tgagcgtcgg
     6241 gatcgcgttc aacaaacggt tcggcgcggg gctgttcggc ggtgaaggct tcatcatgga
     6301 gcgcctgacc ggcgacggct ggtgcttcgt gcacgccgga ggcacgctcg agaagcggca
     6361 gctcgcggcg ggcgagacgc tccgcgtgga cacgggatgc ctcgtggcgc tccagccctc
     6421 ggtctcgtac gacatccagt acgtgggcaa gctgaagagc gcgctcttcg ggggcgaggg
     6481 gctcttcttc gcgacgctcc gagggcccgg aaccgtgtgg ctccagtcgc tcccgttcag
     6541 ccgactcgcg agccgcatct atgccgccgc tccgcaggcc ggtggccgcc ggcgcgagga
     6601 aggctcgctc ctgggcggtc tcggaaatct cctcgacggc gacgggcagt agcgcatcgc
     6661 cgagaacgaa catgggcgct gcgcctacct gcggcgcagc gcccagaata gaagcgagag
     6721 gaccaagctc aagaggagcg acgtcccgag cgggatgtag accttccagt tcgacccgcc
     6781 gaagcggaaa tccccgggca aacgcccgag ccacggcatg cgcggccccc acgtcaggac
     6841 cgctcctacg aggaagagcg cggctccaag aacgaggagc acgcgccccc aggattcgtt
     6901 cattcctcga cggtacgctc agggctttcg ctcccgcaag gtgatggtgc ccgagccggt
     6961 ctcgatgccg atcgtgccgc gcccgtcccc gatccgtccg accagggcgt cgtgcttccg
     7021 cacctgcgtc tcgagggcca gctcggaacg aatcccgccg ctgcccgatt ccaccgagac
     7081 ctgcgcgccg acgccgggcg ggaaggagac ggtgatgtc
//
LOCUS       DASTKC010000007         6930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1898628, whole genome shotgun sequence.
ACCESSION   DASTKC010000007 DASTKC010000000
VERSION     DASTKC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898628"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2086
                     /locus_tag="VFR25_00295"
     CDS             <1..2086
                     /locus_tag="VFR25_00295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9939516.1"
                     /translation="RLLIAALAAGALMADPAAAQTATHSTTSKTTTTKSKTAAAKSTG
                     AKTAMPGRFFTGKPNAARYRQLSTQELGLARADLKKLLAVKGKRTVQNTMTLYSSLTL
                     HADNAGSWAGLMESVHPDSAFRSTAEAMSQDASKFFTELGLNRQLYEAIQAVDVSAAD
                     PTTKYLVEKTLRDFRLAGVDKDDATRKQIQAIQEELVLVSQEFDRNIRNDSRTIKVAP
                     ADLDGMPDDFLKAHPPGADGKVTLSIEYPDRFPIATYAKKEDVRKRLQFEAMNRAYPA
                     NEAVLDSMLDKRYRLARMLGYPNWADYITANKMIGNAGNAAQFIDRIGKLTREQSQRE
                     YEIYLKRKQEDVPGATAVNRWEVRYYEQLIRKRDYNFDPSAARPYFPFEGVKKGVLDV
                     TSKLYGVTYKRIQHPDVWDPSVEAYDVYDGKTLLGRFFFDLHPRPGKFNHAAQFNIRT
                     GVTGAQLPEACLVCNFPGGTKGDPGLMEHDDVVTFFHEFGHLLHSIFGGHQRWAPLAG
                     IATEQDFVEAPSQMLEEWAWDPAVLRTFAKHYQTGEAIPVEMVENLRKASVFGRAIDN
                     AYQADYAALSLNIYNRDPASVNVDSLSVAYDRDYVPFPPIPDTHYAMSFGHLDGYSAV
                     YYTYLWSLVIAKDLFTKFDRDHMLEPGAATRYRKVVLAPGGTKPAAQLVHDFLGRDFQ
                     YDAYEQWLAGKN"
     gene            complement(2148..2639)
                     /locus_tag="VFR25_00300"
     CDS             complement(2148..2639)
                     /locus_tag="VFR25_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9939517.1"
                     /translation="MGAPDKKPTDSLRQDHVRLLTQIHGLGERLEKLHELGTLEQLAV
                     AREVLEFVSKNVAPHARAEEYTLYPAADWAAGEGSRLTEIARFEHQLVARRCQALEKA
                     VSAGSPAGRLMHLSYGILALIAAHFQATEEVILPYLDKAFDGTRFEKEVLTPLRVEKT
                     AKR"
     gene            2759..3211
                     /gene="rpiB"
                     /locus_tag="VFR25_00305"
     CDS             2759..3211
                     /gene="rpiB"
                     /locus_tag="VFR25_00305"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9939518.1"
                     /translation="MKVAIGADHAGFQMKERVGQYLKAKGYTVLDMGTDSTESTDYPQ
                     YAFKVAEAVRDGRADRGVLVCDSGNGIAIAANKVEGIRAALAVNPKHAELSRRHNDAN
                     VLVLGSMLTPESEERPLLDAWFSAPFDGGRHERRVGQITDYERTHCKG"
     gene            3219..4970
                     /locus_tag="VFR25_00310"
     CDS             3219..4970
                     /locus_tag="VFR25_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9939519.1"
                     /translation="MTRPVVQLENLGSFEPHFQAALDELSKTRALQRLVERDPTLWKS
                     DAAHEAVIRNRLGWLDAPQWLAGKIDELTAFAAEIRNAGFTRVVLLGMGGSSLAPEVF
                     QIVSIAGPGAPTLDVLDSTDPATIDDIEGASRLDRTFFLVSSKSGKTIETLSQYAYMR
                     HRLDASKIENPGRQFAAITDEGSPLDRLAQDEGFRRVFRNPGDIGGRYSALSYFGMVP
                     AAILGLDLRVLHMRAAAARKESLEPESARNIAFRLGAYLGGGARAGRNKLTIETAKAL
                     RPLGYWIEQLVAESTGKEHTGVVPIEGEPLGPAHHYGDDRLLLTIRLQGEPDADLDRL
                     EGEWTRTGAPWLRIDLPDRDAIAGEFYRWEVAVALAGALLHIDPFDEPNVQESKDATQ
                     RILDELAKVGRLPTGEPRSLSAGIEIHATDEVWKRIEADVPSMPSLEMILNRFLGLAE
                     PHSYLAVLAFIERTAASEASFALLRRAIRNAIHVPVLQGYGPRYLHSIGQLYKGGPNE
                     GMFLILTTEHKSDLPIPGKPFTFGQLETAQALGDYEALAAHGRPALRLHFSHGVDAGL
                     ATLAAAVERALSAMQGA"
     gene            5125..6375
                     /locus_tag="VFR25_00315"
     CDS             5125..6375
                     /locus_tag="VFR25_00315"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9939520.1"
                     /translation="MLVLLGRAASAGQSPIITAPPLVEAAEQDSIRFTVSATDPDGDL
                     ITGLGVLQLPTGAVFDVNLGASRGTFRWRPDYGSAGSYPLSFYAENSVRSYGYTQLQI
                     NNTDRPPVIAVPSSITAVEGQALEFTASVTDPDGDSFLSLVAEALPEGAQFTTNPSQS
                     FARITWTPQPGQGGSYKVRFVSTTRPLLGPSGVGTAAVTIAVAGGQYSPRVFALGDEK
                     TVLMGPGTGQVCIHIEPRGTYFDPSLIVPSTVELIWSAGLHLPADEVDLNEEDVDGNR
                     VKDVTACFSRNALKHLLANEELPRTSRVTVRGNLVGGGAFSGVVDLNPYLASTRVFLT
                     PNPSRGNAVLSFHTSDTGRTQLRIFDSRGRLLRTLVDEPYMRAGWHEVRLEGGSSKHL
                     PTGMYFYKLDTIDTSLKGQFLIVK"
BASE COUNT         1210 a         2409 c         2190 g         1121 t
ORIGIN      
        1 ccgattgttg atcgcagctc tcgccgcggg ggccctgatg gcggacccgg ccgcggccca
       61 aacggccacg cactccacca cttcgaagac cacgacgacc aagagcaaga cggccgccgc
      121 caagtccacg ggcgccaaga cggccatgcc cggccgcttc ttcaccggca agccgaacgc
      181 ggcgcggtac cgccagctct ccacgcagga gctgggcctc gcgcgcgccg atctgaagaa
      241 gcttctcgcg gtgaagggca agcggacggt ccagaacacg atgacgctct actcgagcct
      301 gacgctccac gccgacaacg ccggcagctg ggccgggctc atggagtcgg tgcatcccga
      361 ctccgcgttc cgatcgacgg ccgaggcgat gagccaggac gcgagcaagt tcttcacgga
      421 gctcggactc aaccggcagc tctacgaagc gatccaggcg gtcgacgtga gcgccgcgga
      481 tccgaccacg aagtatctcg tcgagaagac gcttcgcgac ttccggctcg ccggcgtcga
      541 caaggacgat gcgacgcgga agcagatcca ggcgatccag gaggagctgg tcctggtctc
      601 gcaggaattc gatcggaaca tccggaacga ctcgcgcacg atcaaggtgg cgcccgccga
      661 cctcgacggc atgccggacg acttcttgaa ggcgcacccg ccgggcgccg acggcaaggt
      721 cacgctctcg atcgagtatc cggaccgctt ccccatcgcg acgtatgcca agaaggagga
      781 cgtgcgcaag cgcctccagt tcgaggccat gaatcgcgcc tatccggcga atgaagccgt
      841 gctcgactcg atgctggaca agcgctaccg cctcgcccgc atgctcggct acccgaactg
      901 ggcggactac atcaccgcga ataagatgat cgggaacgcg gggaacgcgg ctcagttcat
      961 cgaccggatc ggcaagctca cgcgggagca gagccagcgc gagtacgaga tctacctgaa
     1021 gcgcaagcag gaggacgttc ccggcgcgac cgcggtcaat cgctgggagg tgcgctacta
     1081 cgagcagctc atccggaagc gcgactacaa cttcgatccc agcgccgccc gcccgtactt
     1141 ccccttcgag ggggtgaaga agggcgtgct cgacgtcacg tccaagctct acggcgtcac
     1201 ctacaagcgc atccagcacc cggacgtgtg ggatccgagc gtcgaggctt acgacgtcta
     1261 tgacgggaag acgctcctgg gccggttctt cttcgacctc catccgcggc ccgggaagtt
     1321 caaccacgcc gcccagttca acatccggac cggcgtcacg ggagcgcagc ttcccgaggc
     1381 gtgtctcgtc tgcaacttcc cgggcggtac gaagggcgat cccgggctca tggagcacga
     1441 cgacgtcgtg accttcttcc acgagttcgg ccatctcctg cactcgatct tcggcggcca
     1501 tcagcgctgg gcgccgctgg ccggcatcgc cacggaacag gacttcgtcg aagcaccgtc
     1561 ccagatgctc gaggagtggg cgtgggatcc cgcggtgctc cggacgttcg ccaagcacta
     1621 ccagaccggg gaggccattc cggtcgagat ggtcgaaaat ctccgcaagg cgagcgtctt
     1681 cggccgcgcg atcgacaacg cgtaccaggc cgactatgcg gcgctctcgc tcaacatcta
     1741 caatcgcgat cccgcgtccg tgaacgtgga ctcgctgtcg gtggcgtacg accgcgatta
     1801 cgtccccttc ccgccgatcc cggacacgca ctacgcgatg tccttcggtc atctcgacgg
     1861 ctactcggcg gtctactaca cgtatctctg gtcgctcgtg atcgcgaagg atctcttcac
     1921 caagttcgac cgcgaccaca tgctggagcc cggcgcggcg acgagatatc gaaaggtcgt
     1981 gctcgcaccc ggcggcacca agcccgcggc gcagctcgtg cacgatttcc tgggccgcga
     2041 cttccagtac gacgcgtacg aacagtggct cgcggggaag aactaggggc gggagcctga
     2101 gcagcggttc ggcgtagatg agggtcgtcg cccacgggcg gcggccctca gcgcttggcg
     2161 gtcttctcca ccctcaaagg ggtgaggact tccttctcga aacgggtgcc gtcgaaggcc
     2221 ttgtcgagat agggcaggat cacttcctcg gtcgcctgga agtgggcggc gatgagcgcg
     2281 aggatgccgt aggagagatg catcaagcgg ccggccggcg agccggccga gacggccttc
     2341 tcgagtgcct gacagcgccg ggcgacgagc tgatgctcga aacgggcgat ctcggtcagc
     2401 cgcgatccct cccccgctgc ccagtcggcc gccgggtaga gggtgtactc ctcggcgcgg
     2461 gcgtgggggg cgacgttctt cgagacgaac tcgaggacct cccgcgcgac cgcgagctgc
     2521 tccagggtcc ccagctcgtg cagcttctcc agccgctccc ccaggccatg aatctgggtc
     2581 agcaggcgga cgtgatcttg cctcagggag tcggtcggct ttttgtcggg ggcccccatc
     2641 gccgggaact attcgacttc cgcgtctcgt tgtcaactcg actgttgcgc cgaccctggc
     2701 cctgacctag cctcgaatga tgaccccttc ggaatcgacc tcgaaaggag cctccccgat
     2761 gaaggtcgcg atcggtgccg accacgcggg cttccagatg aaggagcgcg tcgggcagta
     2821 cctgaaggcg aagggctaca ccgtgctcga catgggcacc gactccaccg agtcgaccga
     2881 ctatccgcag tacgcgttca aggtggcgga ggcggtccgg gacggccgcg ccgaccgcgg
     2941 cgtgctcgtc tgcgattccg ggaacggcat cgcgatcgcg gccaacaagg tcgagggcat
     3001 tcgcgccgcc ctcgccgtca atcccaagca cgccgagctg tcgcgccgcc acaacgatgc
     3061 gaacgtgctc gtcctcggat ccatgctcac gcccgagtcc gaggagcgcc cgctcctcga
     3121 cgcgtggttc tccgctccct tcgacggtgg ccgccacgag cggcgggtgg gtcagatcac
     3181 cgactacgag cgaacccatt gcaagggttg atccgcacgt gactcgccct gtcgtccagc
     3241 tggagaacct gggctcgttc gagccccact tccaggcagc cctcgacgag ctctcgaaga
     3301 cccgtgcgct ccagcgtctc gtggagcggg acccgacgct ctggaagagc gacgccgcgc
     3361 acgaagccgt catccggaat cgcctcggct ggctggatgc cccgcagtgg ctcgccggca
     3421 agatcgacga gctcaccgcg ttcgcggccg agatccggaa cgccggcttc acgcgcgtgg
     3481 ttctcctcgg gatgggcggc tcgagcttgg cgccggaggt cttccagatc gtctccatcg
     3541 cgggaccggg tgcaccgacg ctggacgtcc tcgactccac ggacccggcg accatcgacg
     3601 acatcgaagg ggcgagccgg ctcgaccgga cgttcttcct cgtctcgagc aagtcgggca
     3661 agacgatcga aacgctctcc cagtacgcgt acatgcgcca ccggctcgac gcctcgaaga
     3721 tcgagaaccc tgggcgccaa ttcgcagcca ttacggacga aggttctccg ctcgaccgtc
     3781 tcgcgcagga cgagggattc cgccgcgtgt tccgcaaccc gggcgacatc ggcggtcgct
     3841 attccgcgct cagctatttc ggcatggttc ccgccgcgat cctgggcctc gacctccgcg
     3901 tgctccacat gcgcgccgcg gccgcgcgga aggaatcgct cgagccggaa tcggcgcgga
     3961 acatagcgtt ccggctgggc gcctatctcg gcggcggcgc gcgggccggc cggaacaagc
     4021 tcacgatcga gaccgcgaag gcgctgcgcc cgctcggcta ctggatcgag cagctcgtgg
     4081 ccgagagcac gggcaaggag cacaccgggg tcgtgccgat cgagggcgag ccccttggtc
     4141 ccgcccatca ctacggggac gaccggctcc tgctcacgat ccggcttcag ggcgagcccg
     4201 atgcggatct cgaccggctc gaaggcgaat ggacgcgcac cggcgcgccg tggctccgca
     4261 tcgatcttcc cgatcgcgac gcgatcgcgg gcgaattcta tcgatgggaa gtggcggtcg
     4321 cgctcgcggg agcccttctc cacatcgatc ccttcgacga accgaacgtg caggagagca
     4381 aggacgcgac gcagcggatc ctggacgagc tcgccaaggt aggccggctc cccacgggcg
     4441 agccgcgctc gctgagcgcc ggcatcgaga tccacgcgac cgacgaggtc tggaagcgga
     4501 tcgaggccga cgtcccctcg atgccgagcc tcgagatgat cctgaaccgg ttcctcggcc
     4561 tggccgagcc gcacagctac ctcgccgtgc tcgcgttcat cgagcgcacc gcggcgagcg
     4621 aagcctcgtt cgcgctccta cggcgggcga tccggaacgc gatccacgta ccggtgctcc
     4681 agggctacgg tccgcgctac ctccactcca tcgggcagct ctacaagggc gggcccaacg
     4741 aggggatgtt cctgatcttg acgacggagc acaagtcgga cctccccatt cccgggaagc
     4801 cgttcacctt cggtcagctc gagaccgcgc aggcgctggg agactacgag gcgctcgcgg
     4861 cccatggacg gcccgcgctc cgcctccatt tctcccatgg ggtcgacgcc ggcctggcca
     4921 ccctcgcggc cgccgtggag cgcgccctgt ccgccatgca gggggcgtag ccataccgcc
     4981 tctgttccgg taatctcaga gggcccccaa gctccgatat ccaggccacc gccggaacgc
     5041 cctgggtcca cggccttgcg aagattcgcc gatccgcaac gcatacgtag gctccgcccg
     5101 cgagcttcag tcgcttgcgc cctgatgctg gtcctccttg ggcgagctgc gtcggccggt
     5161 cagagcccga tcatcacggc gccgccgctg gtggaggcgg ccgagcagga ctcgatccgc
     5221 ttcacggtat ccgccaccga ccccgatggc gatctcatca ccggactcgg tgtgctccag
     5281 ctgccgaccg gcgccgtgtt cgatgtcaat ctcggcgcct cgagaggcac cttccggtgg
     5341 cgacccgact acggaagcgc cgggtcctac cccctttcgt tctatgcgga gaattcggtc
     5401 cgcagctatg gctacaccca gctccagatc aacaacacgg atcgccctcc ggtgattgcc
     5461 gtcccctcgt ccatcacagc cgtcgagggg caggccctgg agttcaccgc gtccgtgacg
     5521 gacccggacg gagacagctt cctctccctc gtggccgagg cgcttccgga aggcgcccag
     5581 ttcaccacga atccatccca gtcgttcgcc cgcatcacat ggacgcctca gccgggccag
     5641 ggagggtcgt acaaggtgcg gtttgtctcc acgacgcgtc ccctcctcgg gccgtctgga
     5701 gtcggcacgg cagccgtcac gatcgcggtg gccggcgggc aatattcgcc acgcgtgttc
     5761 gcgctgggcg acgagaagac cgtgctcatg gggccgggaa cgggtcaggt gtgcatccac
     5821 attgaaccca gaggcacgta cttcgacccc agcctgatcg tcccgtccac cgtggagctc
     5881 atctggagcg ccggactcca tcttcccgcg gacgaggtcg atctgaacga agaggacgtc
     5941 gatgggaatc gggtgaagga cgtgaccgcg tgcttctcga ggaacgccct gaagcatctc
     6001 ttggcaaacg aggagctccc caggacctct cgcgtcacgg tccggggcaa cctggtcgga
     6061 ggaggcgcct tcagcggagt cgtcgacctg aatccctatc tggcgtcgac ccgggtgttc
     6121 ctgactccca atccgagccg cggaaacgcg gtgctctcgt tccatacgtc cgacaccggt
     6181 cggacccagc tccggatctt cgactcgagg ggccggctcc tccgaacgct cgtcgacgag
     6241 ccgtacatgc gggccggctg gcacgaagtt cggctcgagg gcggctcgtc gaagcacctt
     6301 cccaccggca tgtacttcta caagctcgac acgatcgaca ccagcctcaa gggacagttc
     6361 ctgatcgtga agtgaggtgc cgttttccca catcccgcaa gctttacagc agtacaattt
     6421 gcccctccat gcaccttgct cgagccggct tcctcctgtt tttcgtgatc gttgtcccag
     6481 cgccttgcgc tggcgcgtcg cctgcaccgc cctcactcgc cgccggtccc gcgcgccaac
     6541 taaggccacg tcaccatcaa accaagcctg cccacctcgt gattcgaccc ggctttgact
     6601 cgcgtcggat atctgtgaaa tttcaggatg ggctccaact ggaaatcgat cacataggcc
     6661 ctggtggatt gcacgcaccg agcatgaatt cacgggctct gaacgtgctc cgcttgatcc
     6721 gagaggcagg aggacggtgg cggtccgcct cgaccatgag cggcagacgg ctggctgagc
     6781 tcagatcacg ggccgaggtc gcaatgggaa gagagatcgc cgatctctcc gattacttca
     6841 ccctgacgct ccccccagtg gcggacgctg agggattgat cgacgcactc aacgcgctcc
     6901 ccgaagtgga gatggctgcc cccacacctc
//
LOCUS       DASTKC010000008         5745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_481086, whole genome shotgun sequence.
ACCESSION   DASTKC010000008 DASTKC010000000
VERSION     DASTKC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481086"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..265)
                     /locus_tag="VFR25_00320"
     CDS             complement(35..265)
                     /locus_tag="VFR25_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9939521.1"
                     /translation="MKALIEYCARQLVDHPEGVHVDEHETGDTTVLTLRVSAGDLGKV
                     IGREGRTAQSLRTLLAASAAAQGKRATLEIAD"
     gene            complement(289..765)
                     /gene="rpsP"
                     /locus_tag="VFR25_00325"
     CDS             complement(289..765)
                     /gene="rpsP"
                     /locus_tag="VFR25_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9939522.1"
                     /translation="MSVVIRMRRTGAKGRPAYRVVVADSRYPRDGRYIEQLGYYDPLT
                     NPATFQINAERLSMWIQRGALPSESVGVMVAKHAPDALRKPLQPATPAPELKEVPAPK
                     KKVAKKAAAKKKTVKPAAKATKGGEKKAKTRAKAKARKASGVAKPRATKKAKKKKS"
     gene            complement(809..2137)
                     /gene="ffh"
                     /locus_tag="VFR25_00330"
     CDS             complement(809..2137)
                     /gene="ffh"
                     /locus_tag="VFR25_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9939523.1"
                     /translation="MFEELTDRLSGVFKKLLGRGRLSEQDVKESLREIRRVLLEADVN
                     LNVARDFLKAVEARAVGEDLLSSVTPGQLIVKIVHEEMVKLLGGGAATPGLQFPSNRA
                     AVILLAGLQGSGKTTTAAKLARYWKGREKRVLLAALDLQRPAAIDQLEVLGKQIGAPV
                     HLDRTTKDPVALGKAARQRADKEGYDLLIADTAGRLHVDEELMAEVVRVQDAIQPNET
                     YLVLDGMTGQDAVSIAESFTKQLKVTGFILTKMDGDARGGAALSLRAVAGAPIRYMGV
                     GEKVDGFEPFHPDRLASRILGMGDVLTLVERAQERLDFSKAEALEKKLKKERFTLEDF
                     LGQLQEMKKLGPLEEIMKMIPGVRLPAGAQVDDREIKRTEAIIQSMTPGERDQPSIIN
                     GSRRKRIAKGSGTQVQDVNRVLRQFEQMQTMLKRFGRGMRGGKMPQGLFR"
     gene            2602..2982
                     /locus_tag="VFR25_00335"
     CDS             2602..2982
                     /locus_tag="VFR25_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939524.1"
                     /translation="MHSPQKIFPGSDRTSWDACDKFGTPGDQIQICASTGLQPGQTVP
                     TYNIRFNMPNAGHVTIGVFDDHARLMKYLLNDDEPATLPGQFRQPPVTWDFTDSEGHR
                     VPDGDYRVYFQTGDFQSTSDVVVE"
     gene            2979..3560
                     /locus_tag="VFR25_00340"
     CDS             2979..3560
                     /locus_tag="VFR25_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939525.1"
                     /translation="MRRALGGVVLAAIGLSFAASALAQPPSTEPPTHGRVTSHLQRTE
                     DERDRFELGMAVPQGYFDWLGTFGYRRFMREGGPLEQSLAAELSGTAKDYLGEGALSV
                     YYLFRPIKSYKEGWKLRPLIEAGPGVHVVVQIADIEGFNDSAFHTQAYLKTHLYGGFE
                     FLFSQKLGLVVRGRVTLPEDHPFDYAQAAILLR"
     gene            complement(3530..>5745)
                     /gene="uvrA"
                     /locus_tag="VFR25_00345"
     CDS             complement(3530..>5745)
                     /gene="uvrA"
                     /locus_tag="VFR25_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9939526.1"
                     /translation="PKRVLDAGPTTATALGPTADPIEVFVDRLAATPDNRMRLREALE
                     LAWRESLGSVQVARESGETLTFFDGRTCARCGKTFPEPRPQLFSFNSPYGACDDCHGF
                     GNILTFTVERVVPDPNKSILNGALDPWANSWRAHFMPKVQALAKEHGIPLGVPFRTLS
                     KEHQKMLLHGTTGFRGVFPFLERLKEKAYKSSNRFLVKRYQESVLCQTCGGMRLKPEA
                     LDVRIGSRNVAEISAMTVGQLRHFMATLELDPVRSAVAAPILAELRGRLEYLGETGLD
                     YLTLDRPSKTLSGGEAQRIDLANALGGSLSHTLYVLDEPTVGLHPRDTERLIRVLKRL
                     RERGNTLLVVEHDPDVIASADWILEMGPGAGHRGGSLLYEGPSQTWPAREETTQPVEI
                     AAERKPFYRTQPTEWIEIVGAREHNLKGIDVRFPLGAFTGVCGVSGSGKSTLVEEILW
                     RVAARKMGENVPAPGAHKITRGLGQFDRVTLVDQSPVARSTRSNPSTYVKAFDRIRER
                     YARAPLARQRRYTPGTFSFNVKGGRCETCEGAGVTKIEMFFLADLWVPCETCNGRRYR
                     PEILEVKVHGLAIDELLDKTVEETLALFPGETEIQEPLWILERVGLGYLKLGQSLSTL
                     SGGETQRLKLARELSGRNAEESLYILDEPTVGLHRKDVATLLKVLRELTRRGATIIAV
                     EHNLDFLAACDYLVELGPEGGDAGGKLVAEGTPAELSRVEGSPTASYLRRIAACA"
BASE COUNT          848 a         1989 c         1889 g         1019 t
ORIGIN      
        1 gccgaccagg atctcgtcgc ccgacccagc gtcgctaatc cgcgatctcc aacgtagccc
       61 gcttcccctg cgccgcggcc gacgccgcga ggagcgtgcg gagcgactgc gcggtgcggc
      121 cctcccggcc gatcaccttg cccagatctc cggccgacac gcggagcgtg agcaccgtgg
      181 tgtctccagt ctcgtgctcg tccacgtgca ccccttcggg gtgatcgacg agctggcgcg
      241 cgcagtactc gatcagggct ttcacgaccg cctccttgcc tcgcgggctc aggacttctt
      301 cttcttcgcc ttcttggtcg ccctaggctt ggcgacgccg ctcgccttgc gggccttcgc
      361 cttggcgcgc gtcttcgcct tcttctcgcc gcccttggtc gccttggccg ccggcttcac
      421 ggtcttcttc ttcgccgcgg ccttcttcgc gaccttcttc ttgggcgcgg gaacttcctt
      481 gagctcgggc gcaggcgtcg cgggctgaag cggcttccgc agcgcgtccg gcgcgtgctt
      541 cgccaccatc acgccgaccg actccgacgg aagcgcgccg cgctggatcc acatcgacag
      601 gcgctccgcg ttgatctgga acgtcgccgg attggtgagc ggatcgtagt acccgagctg
      661 ctcgatgtag cggccgtccc tcgggtagcg ggaatcggcg acgaccacgc ggtaggccgg
      721 gcgtcccttc gccccggtgc gtctcatgcg aatcacgaca gacattccga atctcctcct
      781 cggtgtgaca cgatcgccgg acgctcgctc agcggaagag cccctgtggc atcttcccgc
      841 cgcgcatgcc gcgcccgaag cgtttcaaca tcgtctgcat ctgctcgaac tgccggagca
      901 cgcggttcac gtcctgcacc tgcgtgccgc tgcccttcgc gatgcgcttg cggcggctgc
      961 cattgatgat cgacggctga tcgcgctcgc ccggcgtcat cgactggatg atcgcctcgg
     1021 tgcgcttgat ctcgcgatcg tccacctgcg cgcccgcggg gagccgcacc ccggggatca
     1081 tcttcatgat ctcctcgagc ggccccagct tcttcatctc ctggagctgc ccgaggaaat
     1141 cctcgagggt gaaccgctcc ttcttcagct tcttctcgag ggcttccgcc ttcgagaagt
     1201 ccagccgctc ctgggcgcgc tcgaccagcg tgagcacgtc ccccatcccg aggatccggg
     1261 acgcgagccg gtcgggatgg aacggctcga agccgtccac cttctcgccc acccccatgt
     1321 agcggatggg cgcgcccgcg accgcgcgga gcgagagcgc cgcgccaccc cgcgcgtcgc
     1381 cgtccatctt ggtcaggatg aagccggtca ccttgagctg cttcgtgaac gactcggcga
     1441 tcgagaccgc gtcctgaccc gtcatcccgt cgagaacgag gtacgtctcg ttcggctgga
     1501 tggcgtcctg cacgcgcacc acctcggcca tcagctcctc gtccacgtgg agccggcccg
     1561 cggtatccgc gatcaggagg tcgtagcctt ccttgtccgc gcgctgccgc gccgccttgc
     1621 cgagcgcgac cggatccttg gtcgtgcgat ccaggtgcac cggcgcgccg atctgcttgc
     1681 cgagcacctc gagctgatcg atcgccgcgg gacgctgaag gtcgagcgcc gccagaagga
     1741 cgcgcttctc gcgtcccttc cagtagcgcg cgagcttcgc cgccgtggtc gtcttgcccg
     1801 agccctgaag ccccgcgagc aggatcaccg cagcgcgatt cgacgggaac tggagccccg
     1861 gagtcgccgc gccgccgccg aggagcttca ccatctcctc gtggacgatc ttcacgatca
     1921 gctgcccggg cgtgacgctc gagagcagat cttcgccgac cgcgcgcgct tcgaccgcct
     1981 tcaggaagtc gcgggcgacg ttcaggttca cgtcggcctc gaggaggacg cgccggatct
     2041 cccggagcga ctccttgacg tcctgctcgc tcagccgtcc ccggccgagc aatttcttga
     2101 aaacgcctga taggcggtcg gttagctctt cgaacatggg cgtccttggc ctgggggagg
     2161 caagggccac ccctggagcg gcaaacgtcg aagggtatca gcgaggcagc gggttggcaa
     2221 ggcaggactt ggggatggtg cccggcgccc tgtcggggtc caggtctata cgtagatgta
     2281 cgcgagacgc gacgatttgg ggggccgaca taaaaatgtt tgacacccct tttccacggt
     2341 tgctagcgtc atgggtgagc ggagttccct gccgcctcgt gcccaatccg cagccaaaac
     2401 ctagtccttc cagcgggttt cttcttgcgg ggttttgatt tgcgaattta cgtggatgcg
     2461 cggggaactc tctcactgta atctctgtca agaacggcac cgtctgggca acaccccccc
     2521 gtcgccggaa taggagcggt tccactgcgg attgtcttcc ttctggcggc tctcgctatc
     2581 gcgggctgta gcagcgacgg cgtgcactcc ccgcaaaaaa ttttcccggg gtccgaccgc
     2641 accagctggg acgcgtgcga caagttcggc acgcccggcg accagatcca gatctgtgcc
     2701 tcgaccgggc ttcaacccgg acagaccgtc cccacgtaca acatccgctt caacatgccc
     2761 aacgcgggac acgtgacgat cggcgtcttc gacgatcacg ccaggctgat gaagtacctc
     2821 ctgaacgacg acgagcccgc gaccttgccg ggccagttcc gccagccgcc ggtcacctgg
     2881 gacttcaccg actccgaggg ccatcgcgtc cccgacggcg attaccgcgt gtacttccag
     2941 acgggcgact tccaatcgac gagcgacgtg gtcgtcgagt gaggagagcc ctggggggtg
     3001 tggtgctcgc ggcgatcggg ctttccttcg ccgcgtccgc cctggcccag ccgccgtcga
     3061 cggagccgcc cacgcacggg agggtgacct cgcatctcca gcgcacggag gatgagcgcg
     3121 accgcttcga gttgggcatg gccgtgccgc agggctactt cgactggctg ggcacgttcg
     3181 gctaccggcg gttcatgcgc gaaggcggac cgctcgagca atcgctcgcg gccgagctga
     3241 gcggcaccgc caaggactac ctcggcgagg gggcgctcag cgtctactac ctcttccggc
     3301 cgatcaaatc gtacaaggaa ggctggaagc tacgtcccct gatcgaggcg gggccaggcg
     3361 tgcacgtcgt cgtgcagatc gcggacatcg agggcttcaa cgattccgca ttccacacgc
     3421 aggcgtactt gaagacccat ctctacggcg gattcgagtt cctgttcagc cagaaattgg
     3481 gactcgtcgt gcggggccgc gtgaccctgc cggaggatca tcccttcgac tacgcgcagg
     3541 ccgcgattct cctgaggtag ctcgcggtcg gggatccctc gacccgcgac agctccgccg
     3601 gcgtcccctc cgccaccagt ttccctcccg cgtccccccc ttccggtccc aattccacga
     3661 ggtagtcgca cgcggcgagg aagtcgaggt tgtgctccac cgcgatgatc gtcgcgccgc
     3721 ggcgcgtcag ctcgcggagc accttgagga gcgtggccac gtccttccga tggagcccga
     3781 ccgtgggctc gtcgaggatg tagagcgatt cctccgcgtt ccggcccgac agctcgcgcg
     3841 cgagcttgag ccgctgcgtc tcgccgccgg agagggtgga gagcgactgg ccgagcttga
     3901 ggtacccgag ccccacgcgc tcgaggatcc agagcggctc ctggatctcc gtctcgcccg
     3961 ggaagagcgc gagcgtctcc tcgacggtct tgtcgagcag ctcgtcgatc gcgagaccgt
     4021 ggaccttcac ctcgaggatc tcggggcggt atcggcggcc gttgcaggtc tcgcagggca
     4081 cccagaggtc ggcgaggaag aacatctcga tcttggtcac gccggcgccc tcgcacgtct
     4141 cgcaccggcc tcccttcacg ttgaacgaga aggtccccgg cgtgtagcgg cgctgccggg
     4201 cgagcggcgc gcgggcgtag cgctcgcgga tgcgatcgaa tgccttcacg taggtcgagg
     4261 ggttggagcg ggtcgatcgc gcgaccgggc tctggtcgac gagggtgacg cgatcgaact
     4321 gccctagccc acgcgtgatc ttgtgcgctc ccggcgccgg gacgttctcg cccatctttc
     4381 gtgccgccac gcgccagaga atctcctcga ccagcgtgga cttccccgac ccgctcacgc
     4441 cgcagacacc ggtgaacgct ccgagcggga accggacgtc gatccccttc aggttgtgct
     4501 cgcgggctcc gacgatctcg atccactccg tgggctgagt gcgatagaag ggcttccgct
     4561 cggccgcgat ctcgaccggc tgcgtggtct cctcgcgcgc gggccaggtt tgggacggcc
     4621 cttcgtagag gagcgagccg cctcggtgcc ccgcgcccgg tcccatctcg aggatccagt
     4681 ccgccgaggc gatgacgtcg ggatcgtgct cgaccacgag gagggtgttg ccgcgctcgc
     4741 ggaggcgctt gaggacccgg atcagccgct ccgtgtcgcg cggatggagc ccgaccgtcg
     4801 gctcgtcgag aacgtagagc gtatggctca ggctcccgcc gagcgcgttc gcgagatcga
     4861 tccgctgcgc ctccccgccc gagagcgtct tggaggggcg gtcgagcgtg aggtaatcga
     4921 ggcccgtctc accgaggtat tcgagacggc cgcgtagctc cgcgaggatc ggcgcggcca
     4981 ccgccgaccg caccgggtcg agctcgaggg tggccatgaa gtgccggagc tgccccacgg
     5041 tcatcgccga gatctccgcc acgttcctgc tcccgatccg cacgtcgagc gcctcgggct
     5101 tgagccgcat cccgccgcag gtctggcaga gcaccgactc ctggtagcgc ttcacgagga
     5161 accggttgct cgacttgtac gccttctctt tgagtcgctc gaggaacggg aacacgcccc
     5221 ggaaccctgt cgtgccgtgg aggagcatct tctggtgctc cttcgagagc gtccggaacg
     5281 gcaccccgag cggaatgccg tgctccttgg ccagcgcctg gaccttgggc atgaagtgcg
     5341 cgcgccagga attggcccac gggtcgaggg cgccgttcag gatcgacttg ttcggatccg
     5401 gaacgacgcg ctcgaccgtg aaggtgagga tgttcccgaa gccgtggcag tcgtcgcacg
     5461 cgccgtaggg gctgttgaag gagaagagct gcggccgcgg ctcggggaac gtcttcccgc
     5521 agcgggcgca ggtccgtccg tcgaagaagg tgagggtctc gcctgattcg cgggcgacct
     5581 ggactgagcc gaggctctcg cgccacgcga gctcgagggc ttcgcggagg cgcatgcggt
     5641 tgtcgggcgt agccgcgagg cgatcgacga agacctcgat ggggtccgcg gtggggccga
     5701 gggctgttgc ggtggtcggg ccggcatcga gcacgcgctt cggtc
//
LOCUS       DASTKC010000009        20574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_8567, whole genome shotgun sequence.
ACCESSION   DASTKC010000009 DASTKC010000000
VERSION     DASTKC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 20574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20574
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8567"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1821)
                     /locus_tag="VFR25_00350"
     CDS             complement(61..1821)
                     /locus_tag="VFR25_00350"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET9939527.1"
                     /translation="MDPSPRHPVPLSAYLIAICVVPVLIAAGFALVALSRHPDNESDR
                     AAADALSGRAPAVERALADESERLERLATVLARDPKFYASLALPKAERGKREYRDAVE
                     SVLRDFQRDALTPIFAVTDERGTLLSRALPPDEPTDLSKAPFVLDAVRGRPGHGVLVE
                     MGMAYCVVAVPVTAGGVVVGSLCLGTALDADVAGTLKDAIGADVMFTIGESVASTTLA
                     PSPIRKSIAQRIGDRSLDTSDLARPRPAAGDRTRPAPDVDVVEVSGARFLAYHRSIDT
                     PIAGGGPLGFVLIRPLGSASSPLVLLRRDLWKASGVGLLSALLAGGAIAGVVFVKRRS
                     AERAHQAEQSSLEARLGAAESLLADVAPTMRETASSIATTASFLASPDLGPLSQPQQE
                     GMDAIARMAGSLERTARDLDDLGGLKRHALEPSLRPADVGALVERATVEQIPAAGRRR
                     QALSFVAEPGLEHDRVDEALFQRAIESIVRQVLSTAPEGARVLIEAGRVEGAIAVAVG
                     ASAALPAPAAGSAEGLQVALARAIMEAHRGALHERDLGEGRRAYVLRLPPTIAAGLPG
                     YFAPPPSAPPTERETLPLAS"
     gene            1917..3329
                     /gene="rlmD"
                     /locus_tag="VFR25_00355"
     CDS             1917..3329
                     /gene="rlmD"
                     /locus_tag="VFR25_00355"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET9939528.1"
                     /translation="MNAPASAPVKKGQSLELEVENLAFGGKALAKLDGFVVFVENALP
                     GDRVQATVFKKRRSYAEARAERILRPSPARVAPRCEHVPTCGGCRFQDLDYAEQLRHK
                     ERQIEECLEHLGRLRVEPRPVVPAPKLYHYRNKMEYSFAKDATGRLTLGLHVRGFFDR
                     TFDLARCHIATPVSSEIVAFVRDAARKAGLTAYDTRSHQGLLRYLTIREGIRTGQVMV
                     NVVASEDHPAIHALAADLPRAFPAVTTVVLNTTRRRAQIATGEEERVLFGRGTIEDVL
                     GGITFEISPNSFFQTNTEQAERLLALALEGLALRGGDRVLDVYSGTGTFTLPIARVAG
                     EAIGVESSAVAVKDAHRNAARNRIENARFVTGEAMEVLRDQLGLGTRASRSAVDRADI
                     DAVLVDPPRAGLHPGVIQRLVHLGAPRLVYVSCNPSTLGRDLALFCASRYRLEWVRGV
                     DMFPHTPHIECVAALRLAEA"
     gene            3343..4629
                     /locus_tag="VFR25_00360"
     CDS             3343..4629
                     /locus_tag="VFR25_00360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9939529.1"
                     /translation="MNPIQAAAARSPVHETTLANGLKVLIQEVRTAPVVSFMVWYRVG
                     SRNEASGITGISHLLEHMMFKGTPTYGKGEIARVLQRHGASFNAGTSLDYTCYYEVLA
                     SDRLELAVELEADRMANALIPEEEHRLEMTVVRSELERNEDNPHRAMFLELLAQSFKA
                     HPYHWPTIGWRTDVEQITTEEIRSYYRTHYVPNNATAVIVGDVDRAKALALVERHFGG
                     IPRGPEPPPVRVVEPPQTGERRFKIRKPGDTRYLVVGWRNPALAHEDNYALDVVGMIL
                     GHGRTSRLYRGLVETKLATEVDAANETARDPFLLVAQATVAPDAALEQVEAALHSEVE
                     LVRTQPVTSEELARAKRQVEASFVYSKDSIRSLAQQLGYFETVASYRYLDTYLERLAA
                     VGPDDVRRVAQAYLTEDARTVGAYDPIPPTNGSNAG"
     gene            4626..5945
                     /locus_tag="VFR25_00365"
     CDS             4626..5945
                     /locus_tag="VFR25_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9939530.1"
                     /translation="MSPAREAGVESPAAQAVRLVLPNGLTILVRENHANPTVALHGVV
                     KAGAIFDPPGKSGLGTFTGGMLDQGTRTRTAFEQASAIESLGASLRFEGGSETVTFHG
                     TMLSEDLEAVLTALADALRNPVFPPDEIEKIRGELLNEYRIAENSTASVAARRANRLL
                     FPPGHPYHRHAGGDEEGIRSITREDLVAHHRAHYGSDSTVIVLVGDVTPDGARSIADR
                     VLGTWAKRPKAIPFEVPEAPAPSRSRRLVVTRPGKSQADVAYAIPGVARTAPDYDAVM
                     MMNYVLGGGSLSSRLMDQIRDRQGLVYGVYSGLMAGIGAGPLQIRAGTNPANAARCAD
                     AILEEVRRLHDEGPTDEEMEEARGYLTGVFPVRLETNAGVAGQLLSAELYGLGLDYVE
                     RYPSIIRSVTTAHARDAARQHLDPEHYVLVFAGPLDPAGLGDVEQDA"
     gene            5938..7200
                     /locus_tag="VFR25_00370"
     CDS             5938..7200
                     /locus_tag="VFR25_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9939531.1"
                     /translation="MRRLAVLLVLGLVLALPSPAHARRVFVPKQHKTIQKAIEAASPG
                     DTVWVSPGVYRGPIVMKKPLTLFADQGPDTTILDGGDSVRVLQIEGVKNAAVIGFTIR
                     RGHANSGGGIACVADTAVMIATCLIEKNWESGISVWQSVDIDMHEIQFVENTGSAIAL
                     DGSGVAIRECLFLKNKGYAGGAITMNRSIFIFPVRTCRFEENSAETGAGGALYADSSQ
                     VLFAECMFLRNHSPLAGGAVAIMNDSRSAISRCVFQENDAKADGAIHADGSMLNVGIC
                     IFDRNRSQAAASAIGYVKRPPSGLQSTLANCTFYQNISGESATIWTEYASPEIRKNIF
                     SLAKGQRVTVAFNGTPLYTCNLIHDPSGAALGSLPSVDTLVGDPLFCDPDHQNFKIRD
                     LSPAALAACGPIGAAPKGCASFKLVPAK"
     gene            7323..7958
                     /locus_tag="VFR25_00375"
     CDS             7323..7958
                     /locus_tag="VFR25_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9939532.1"
                     /translation="MRNFSARVPAAGVLAGLVCLILATPPAFAQRDDEDQGTPPRVRV
                     YHDDDDNGPSASGGGYLGVRVQEITRDLQRARDLPSTQGALVSMVADGSPADQAGIRR
                     GDVIVRVDGNEITDPDELIREMKDEDAGAQVRVVVLRDGSRRTIDVTLGRRPASTDVP
                     PAYRRPPTGDYGELQRRLTALETQQRRLEEEIRALREELRVRNGGRDRDDD"
     gene            8234..9262
                     /locus_tag="VFR25_00380"
     CDS             8234..9262
                     /locus_tag="VFR25_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9939533.1"
                     /translation="MTPMLTIRRAALSLTVAFLVEGVLVGPVPAQEATVQTVPAGEAF
                     GVSEKTQVAPTATTAKRKRHRTRYRSRRRHAQAPLRLPAVTPYGIPRLHVKAAYVFDA
                     TNNRVLFQKNSEQVLPIASLTKLTTAMVFLGTNPDWDRVIEMVPEDIKNSSRTRIRAH
                     EEITVRDLFHASIMSSDNAATKALVRTCGVPYNEFIFRMNLMADSLGLAGTHFVEPTG
                     LNERNVSTAQDLARILWTATQSPVIQAIMQKTDYTFVSNRKLHQLINTNRLLRSQWQI
                     TGGKTGFIRESGYCLATKVKSPEGSDITAIVLGAPSNALRFAEARRILDWTFRFGLTK
                     TETTPESE"
     gene            9324..10808
                     /locus_tag="VFR25_00385"
     CDS             9324..10808
                     /locus_tag="VFR25_00385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9939534.1"
                     /translation="MRITRAGLRVAAAMAVLTAAALHPPRPALAAPPHSPESDDVPIA
                     GAFVYPVGDELDFTRARSGESHGYYVSDPYMAHRGSKNQRIHQGCDLSCGSGGAAVHA
                     IASGTVVVADANALVKTRRKVEVKTTKVVKGKTITKTGWKWKTSTKWRTGWGNYVVLK
                     HVLPSGESVYSLYGHLMPASVCVAQGDIVAAGETIGQVGKTGRASSPHLHLEIRKNAP
                     AGDEERSEDETEIEPTVQDRTFALLATTDPIDFLDKHVRRFEDLEPGSWQARYAFAAC
                     KDGLAAPDEKRFGPDESMDRGEFYRSLVLAFRLSTAFPVGDWTSTVSSLVQAGVLDQD
                     GSEGQKPGDKLTQADGLEMVLRCLERHRARAPNLASLDPMQVGLDFNRRFAGDTEAEV
                     AAKKSKVAAMKATEAKKKAEADRVASARKAAKAAGKKSRVKARTVKPVAPMPILDPGF
                     EALAQSKKTLTRAEGCLLLATTLRAASERMSALQRAAVRVAQSE"
     gene            10814..12112
                     /locus_tag="VFR25_00390"
     CDS             10814..12112
                     /locus_tag="VFR25_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9939535.1"
                     /translation="MIFTRAEGVHVWDQDGRRYLDATSGAFAAQLGYSRPDLVRAMTD
                     AASRLPHARPSIADSEEAASYRKELLAHAGAPYTRAILTSSGSEAVDVALKIAHRYQV
                     ASGHPERHRVLSLAGHYHGQTLAALDVTGWAPRREPYAALLGPLRLGPAPYCRRCFRS
                     LTFPECRLACADAAVDEPPAAAIVETVPAAGLGAPVPPPGWLARLRTLCHGAGALWIA
                     DEVLTGFGRTGDLFAWKRLARRAAPGGGAPDADAVPDLVVFGKGAGAGYAALAGVLVA
                     DRVARTVEPAGFTHQQTYGGHPVAAAVGRAVLRALDEEGVTARVAEREPWLEEQMRSL
                     GGEAGVFDVRGLGFLWGVELAQDAAGTPFPREKAVSERVREACRERGLLIHASHGCAG
                     EDRGDLVLVAPPLVSDDAALTGMVETLRGALRSVVATVEA"
     gene            complement(12113..12448)
                     /locus_tag="VFR25_00395"
     CDS             complement(12113..12448)
                     /locus_tag="VFR25_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET9939536.1"
                     /translation="MKLEGEGQLLRIFIGEADRWHGRSLYEAIVLRAREAGLAGATVL
                     RGLMGFGAKSHLHTAKVLRLSEDLPIVIEIVDRAERIEAFLPVLDEMVADGLVTLERA
                     QVITYRPGK"
     gene            complement(12445..12825)
                     /locus_tag="VFR25_00400"
     CDS             complement(12445..12825)
                     /locus_tag="VFR25_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HET9939537.1"
                     /translation="MLQTLLIGVLGLVGTLARYGLQGVVQRASGGTFPYGTLAVNVLG
                     CFAVGFIATWTLERTGASPLWRSALMIGFCGGFTTFSALAYESFELARAGDVGRGALN
                     LALHAVLGLVAVWGGHVAAMRIAS"
     gene            12881..13633
                     /locus_tag="VFR25_00405"
     CDS             12881..13633
                     /locus_tag="VFR25_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939538.1"
                     /translation="MPLGPARGAWPLAALVTIAGCSYMSDVKTSAPGLSADALRSEKI
                     AIVSVVQVDEVTDVRPPLIASLERVLRATRPDLTIVPAARVAPALDDSTERFLLLGYQ
                     MHGAADPAWLERARPEIRPMARYALLARVEAVKVRHSDRVDVATQTAGQGLQVTGRDV
                     RVGVSLYDLETLKLVFAGEYWGSKEEAIGSRDAPLAEPEDSLGIVGVNDTTTYGVYKK
                     AAPVAQAAEPAFLEFARSLPGAETARTGASVR"
     gene            13630..14436
                     /locus_tag="VFR25_00410"
     CDS             13630..14436
                     /locus_tag="VFR25_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939539.1"
                     /translation="MRSLTAAAASVMLAAALAGCGGGHPKPAPPPAAAVSVRIPGTEL
                     RQGGIAIMGVVEVGEVDQVRPPLVAALDSVLAVKRPEIPRYSQRQVRNAVDDSTARFL
                     LLGYQLHGLVENAWLDRAADSLHDMARFGILARIVRTRVRHDDAEIPVRPGSKETRTV
                     RAAELDAHVQVHVYDLRARTLRASGEFLGIGEHRARPDSVAELPTIRTWVSPEQRTQS
                     MVPPPGGYFEMDPPPLSVAVGPAFAAAIDALFGPPDTTGARTDSTRRSNR"
     gene            complement(14433..15329)
                     /locus_tag="VFR25_00415"
     CDS             complement(14433..15329)
                     /locus_tag="VFR25_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9939540.1"
                     /translation="MSTADLVLLLVCAIWGTTFALLRDSLRSIHPAELMALRFTLATV
                     ILLAVFPRRVFPLKKEALLRGGWIGIFLASGYLTQIIGLATITASRSAFLTCTYILYT
                     PFLAIPMAGEVPRVGEILGILIGFAGLTLFSADAGFSLRAGDLWTLACALSFGIQIVL
                     TNVAGKKVDPVALSVVQMGVGALAGWGIVIARGGFHTPWSQVPWATLVYLAVVATAVV
                     IALQSWALARTTPVRASMIFATEPIFAALFAVAFFGEGMSTREVLGAAIILLGVAVTI
                     LWKPWMERREARRAARVAIDPS"
     gene            complement(15334..16002)
                     /locus_tag="VFR25_00420"
     CDS             complement(15334..16002)
                     /locus_tag="VFR25_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET9939541.1"
                     /translation="MDPRIPTSTIRRLSGYYRVLEELETEGHRTISSQGLAHQSGVTS
                     AQVRKDLSYFGNFGKRGLGYNVPRLRREIRLILGLNRRWKVALVGAGNLGSALYSYKE
                     FRRQGFDIVGVFDMSAERVGQKWRDLAIHHIDELETEAQRLGLEIGVIAVPARAAQAV
                     ADRLVAAGMRGILNFAHRRIQVPPTVALRTVNLSIELESLAFSIKGIHPKPATTNGRR
                     RKTA"
     gene            complement(16003..18441)
                     /gene="gyrA"
                     /locus_tag="VFR25_00425"
     CDS             complement(16003..18441)
                     /gene="gyrA"
                     /locus_tag="VFR25_00425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9939542.1"
                     /translation="MATRDKVIPIYVEDEMRNSYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLVAMRELNLLHDRPFRKSAKITGDVTGNYHPHGTIPVYETMVRLAQSFSMRYPLV
                     DGQGNFGSVDGDAPAAERYTEARLTEFAEEMLRDIEKDTVDMRPNYDETREEPVVLPA
                     AVPNLLVNGSSGIAVGMATNVPPHNLREIVDAIHHVIENPDCPIEDLLRIVQGPDFPT
                     GGVIYGRQGIIDSYTTGRGHITVRSLATIEEMKKDRQAIIVTEIPYMVSKSALLEKIA
                     DLVKDGVLDGISDLRDESDREGMRVVIELKRDAQPKVILNQLFKHTQMQTTFGSNLLA
                     LVGNRPLTLTLKEMIQHYIAHRQEVVVRRTKFDLGEAERRAHILEGFKIALDHIDEIV
                     ALIKASANVEAARQGLMSTFALSELQANAILEMRLSRLTGLERQKIEDEYLEVIQLIE
                     RLRAILDSPAKVLQIIRDDLAEIKERFGDDRRTEIVAATGEFEIEDLIAEEDMVITIS
                     HAGYIKRLPVTTYRSQRRGGRGITGAGTREEDFIEHLFIASTHSYILVYTDRGRVYWL
                     KVHEIPQGGRTAKGKAIVNVVEMSQTERVAAVVPVKGFQEDRYIFLFTRGGTVKKTPL
                     SAFSNPRRGGIVAIGVEPEDALIDAVVTDGTQDIILSKRNGKAIRFKETDVRPMGRTA
                     YGVRGVTLEENDAVVGVIAVRREASILVATEHGYGKRSPISEYRITGRGGKGIISIQT
                     TERNGSVVAALEVVGDDEVMLITRGGIVIRMQVSGISEIGRNTQGVRLIGLEPGDQLI
                     DVARVEESEEDGEA"
     gene            complement(18474..20390)
                     /gene="gyrB"
                     /locus_tag="VFR25_00430"
     CDS             complement(18474..20390)
                     /gene="gyrB"
                     /locus_tag="VFR25_00430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET9939543.1"
                     /translation="MTTAQEGGHSYDARNIQVLKGLEGVRKRPAMYIGSTGQGGLHHL
                     VYEVVDNSVDEALAGFCTEVLVTIHPDTSVTVVDDGRGIPVDMHPSQNRPALEVVMTT
                     LHAGGKFDDNTYKVSGGLHGVGVSVVNALSEWLEVDVYRAGKRYRQRYERGVVASDVE
                     ELGPTDRTGTTVHWKPDPNVFETVDYNFDTLSQRLRELAFLNKGIRIQFLDERTGKKH
                     DFQYEGGIVSFVKYLNENKTVLHPSPISHSRERDKVIVEFALQYNDGYVENVFSFVNN
                     INTVEGGTHLIGFRAALTRTINNYAEREGMLKNLKDLSLGGDDVREGLTAVISVKLPE
                     PQFEGQTKAKLGNTEVKGIVESVVGEGMRTCFEEQPQIAKKIIEKCIATARAREAARK
                     ARDLARRKSILDSGSLPGKLADCQLTDPAQCEIYLVEGDSAGGSAKMGRDRRYQAILP
                     LKGKILNVEKASLDKMLSNEEIRTIITALGAGIDDEFDADKARYHRIIIMTDADVDGA
                     HIRTLLLTFFFRHMRPLIERGYIYIAQPPLYRVAKGKDVRYAYNDAERDAALDDIGRK
                     GVHLQRYKGLGEMNPDQLWATTMDPESRSVLKVTLDDLVEADRMFTILMGDQVEPRRK
                     FIEEFADRVRNLDV"
BASE COUNT         3218 a         7103 c         7021 g         3232 t
ORIGIN      
        1 ggttgggtga gtaatcaaat attgactacg attgtctgcg gatgttgtcg ctttgaggcg
       61 tcagctggct agaggcagcg tctctcgctc ggtgggcggt gcactcgggg gtggggcgaa
      121 atatcccggc aagccggccg cgatcgtcgg cggaagacgg aggacatagg cccggcgtcc
      181 ttcgcccagg tcgcgttcgt ggagcgcgcc gcgatgtgcc tccatgatcg cccgagcgag
      241 cgcgacctgg aggccctcgg cggatccagc cgccggagcg ggcagcgcgg cgcttgcgcc
      301 gactgccacc gcgatcgccc cctcgacccg tcctgcctcg atgagcaccc gcgctccctc
      361 aggggcggtg gagagcactt gccggacgat cgattcgatc gcgcgctgga agagcgcctc
      421 gtccacccgg tcgtgctcga gtcctggctc ggcgacgaag ctcaacgcct ggcggcggcg
      481 ccctgcggct ggaatctgct cgaccgtggc ccgctccacc agcgcgccga cgtccgccgg
      541 ccgaagactc ggctcgagag cgtgccgctt gaggccgccc agatcgtcca ggtcccgagc
      601 cgtccgctcg agcgacccgg ccatccgagc gatcgcgtcc attccctcct gctgcggctg
      661 ggatagcggg ccgaggtcgg gcgacgctag gaacgaggcg gtcgtggcga tcgagctcgc
      721 tgtctcgcgc atcgtggggg ccacgtccgc gaggagcgac tcggcggcgc cgagcctggc
      781 ttcgagcgag ctctgctccg cctggtgcgc gcgctccgcg ctccgcctct tgacgaagac
      841 gacgcccgcg atcgcgccgc cggccaggag cgcggacagg aggcccacgc ccgacgcctt
      901 ccagagatcg cgcctcaaga ggacgagcgg cgaggaagcc gacccgagcg ggcggatcag
      961 gacgaacccg agcgggccgc ccccggcgat cggcgtgtcg atcgagcggt gataggcgag
     1021 aaagcgtgcg ccggacactt ccaccacgtc cacgtcgggg gcgggccgcg tccgatcccc
     1081 ggccgcggga cgcggccgcg ccaggtcgga cgtgtcgagg ctccgatctc cgatccgctg
     1141 ggcgatcgac tttcggatgg gcgacggcgc gagggtggtg gacgccacgg actcgccgat
     1201 cgtgaacatg acgtcggccc cgatcgcgtc cttgagcgtg ccggccacgt cggcgtcgag
     1261 cgcggtcccg aggcagagcg atccgacgac cacgccgccc gcggtcaccg gaaccgcgac
     1321 cacacagtac gccatgccca tctcgaccag gacgccgtgc cccggccggc cgcgcaccgc
     1381 gtcgagcacg aagggagcct tcgagagatc ggtgggctcg tccggaggga gcgcgcgaga
     1441 gaggagcgtc ccgcgctcgt cggtgaccgc gaagatcggg gtgagcgcgt cgcgctggaa
     1501 gtcgcgaagc acgctctcga cggcatcgcg gtattcgcgc ttccctcgtt ccgccttggg
     1561 gagcgcgagg gacgcgtaga acttcggatc gcgcgcgagg acggtcgcga gccgctcgag
     1621 gcgctccgat tcgtcggcca gggcccgctc caccgcgggc gcgcggccgg agagcgcgtc
     1681 ggcggcggcc cggtcgctct cgttgtcggg gtggcgcgat agagccacga gcgcgaaccc
     1741 ggctgcgatg agcacgggaa cgacgcagat cgcgatgaga taggcgctca agggcaccgg
     1801 atggcgcggg ctcgggtcca cggtgccttg atcggccgct ccgcacttca tctgcagcgc
     1861 gattctggcc ccccgcgctc cggatcggcc cttcgaagct ggtgtaaact ccgcggatga
     1921 acgcccccgc cagcgctccc gtgaagaaag gccagagcct cgagctcgag gtggaaaacc
     1981 tggcgttcgg cgggaaggcg ctcgcgaagc tggacggctt cgtcgtcttc gtcgagaacg
     2041 cgcttcccgg ggaccgcgtc caggccaccg tcttcaagaa gcggcggagc tacgcggaag
     2101 cccgcgccga gcggatcctc cgcccctcgc cggcgcgcgt cgcgccccgt tgcgagcacg
     2161 taccgacctg cggcggatgc aggtttcagg atctcgacta cgcggagcag ctccgccaca
     2221 aggagcggca gatcgaggag tgcctcgagc acttgggacg gctccgcgtg gagccgagac
     2281 cggtcgtgcc ggcgccgaag ctctaccatt atcgcaacaa gatggagtac tcgttcgcga
     2341 aggacgcaac cgggcggctc acgctcgggc tccacgtgcg gggattcttc gaccgcacgt
     2401 tcgatctcgc gcgctgccac atcgcgacgc cggtctcgag cgagatcgtg gccttcgttc
     2461 gcgacgccgc gcgcaaggcg ggactcaccg cctacgacac gcgctcgcac cagggactcc
     2521 tgcgctatct caccatccgc gaggggatcc gcaccgggca ggtcatggtg aacgtggtcg
     2581 cctcggagga ccacccggcc atccacgcgc tcgccgcgga cctgccgcgc gcgttccctg
     2641 ccgtgacgac cgtcgtcctc aacacgaccc ggcggcgcgc ccagatcgcg acgggcgagg
     2701 aggagcgtgt gctcttcgga cgcgggacga tcgaggacgt gctcggcggg atcacgttcg
     2761 agatctcacc gaactcgttc ttccagacga ataccgagca ggccgagcgg ctcctcgcgc
     2821 tcgcgctcga ggggctcgcg ctccgcggag gcgaccgcgt gctcgacgtc tactcgggga
     2881 cgggcacctt cacgcttccg atcgcgcgcg tcgcgggcga agcgatcgga gtcgaatcgt
     2941 ccgctgtcgc ggtgaaggac gcgcatcgaa acgcggctcg gaacagaatc gagaatgcgc
     3001 gcttcgtcac gggcgaagcg atggaggtgc tgcgcgacca gctcggactc ggtacgcggg
     3061 cgtcgagaag cgccgtcgat cgcgcggaca tcgacgccgt gctcgtcgac ccgccgcgcg
     3121 cgggactcca tcccggcgtg atccagcggc tcgtccacct gggcgcgccc cggctcgtct
     3181 atgtctcctg caacccgagc acgctcggac gggatctcgc gctcttctgc gcgtcgcgct
     3241 atcgcctcga gtgggttcgc ggcgtggaca tgttccccca cacgccgcac atcgagtgcg
     3301 tcgccgcgct ccgcctcgcc gaggcgtgat acattcggtg caatgaatcc catccaggcc
     3361 gcggccgcgc ggtcgcccgt ccacgagacg accctggcca acggcctcaa ggtgctgatc
     3421 caggaggtcc ggaccgcccc cgtcgtctcc ttcatggtgt ggtaccgggt cggctcccga
     3481 aacgaggcct ccggaatcac cggcatctcc catctcctcg agcacatgat gttcaagggc
     3541 acccccacgt acgggaaagg ggagattgcg cgcgtgctgc agcggcacgg cgcgtcgttc
     3601 aacgcgggga cgtcgctcga ctacacctgc tactacgagg tgctcgcgag cgaccggctc
     3661 gagctcgcgg tcgagctcga ggcggaccgc atggcgaacg cgctcatccc ggaggaagag
     3721 catcgcctgg agatgacggt cgtgcgctcg gagctcgagc ggaacgagga caatccgcac
     3781 cgcgcgatgt tcctcgagct cctcgcgcag tcgttcaagg cgcaccccta ccactggccg
     3841 accatcggct ggaggacgga cgtcgagcag atcacgaccg aggagatccg gagctactac
     3901 cgcacgcact acgttccgaa caacgcgacc gcggtgatcg tcggggacgt ggatcgcgcg
     3961 aaggcgctcg cgctcgtgga gcgccacttc ggcggcatcc caagaggtcc cgagccgcct
     4021 cccgttcgcg tggtcgagcc gccgcagacg ggcgagcgcc gcttcaagat ccggaagccc
     4081 ggcgacacgc gctatctcgt cgtgggatgg cgaaatccgg cgttggcgca cgaggacaac
     4141 tacgcgctcg acgtggtcgg gatgatcctg ggacatggcc ggacgtcgcg gctctaccgg
     4201 ggcctggtgg agacgaagct cgcgaccgag gtggacgcgg ccaacgagac ggcccgcgat
     4261 ccgttcctcc tcgtggcgca ggcgaccgtg gctccggacg ccgccctcga gcaggtcgag
     4321 gcggcgctcc acagcgaagt ggaacttgtc cggacccagc cggttacatc cgaagagctg
     4381 gcccgggcca agcggcaggt cgaggcctcc ttcgtctatt ccaaggattc catccggagc
     4441 ttggcgcagc aactggggta cttcgagacg gtggcgtcct accgatacct cgacacttat
     4501 ctggagcgct tggccgcggt cggcccggat gacgtgcgcc gggtcgcgca ggcctatctc
     4561 accgaggacg cccgcaccgt gggcgcctac gacccgattc ctccgaccaa cggctcgaac
     4621 gcgggatgag ccccgcccgc gaggccggcg tggaatctcc cgccgcccaa gccgtccggc
     4681 tcgtccttcc gaacggcctc acgatcctcg tgagggagaa ccacgccaac cccaccgtcg
     4741 cgctccacgg ggtggtgaag gccggcgcca tcttcgatcc gccggggaaa agcggcctcg
     4801 gcacgttcac gggcggcatg ctcgatcagg gcacgcgcac gcgcacggca ttcgagcagg
     4861 cgtccgcgat cgagagcctc ggcgcgtcgc ttcgcttcga gggtgggtcc gagaccgtga
     4921 cgttccacgg cacgatgctc tccgaggacc tcgaagcggt cctgaccgcg ctcgccgacg
     4981 cgctccgcaa tccggtgttc ccgcccgacg agatcgagaa gatccggggc gagctcctga
     5041 acgagtaccg gatcgcggag aactccaccg cgagcgtcgc ggcgcgccgc gcgaaccggc
     5101 tcctcttccc tcctggccac ccctatcacc ggcacgcggg gggcgacgag gagggaatcc
     5161 gctcgatcac gcgcgaggac ctcgtcgcgc atcaccgcgc ccattacggc tccgattcca
     5221 cggtgatcgt gctcgtgggc gacgtcacgc cggacggcgc gcgatccatc gccgaccgcg
     5281 tgctcggcac gtgggcgaag cggccgaagg cgattccgtt cgaggtgccc gaggcgccgg
     5341 ctccgtcccg ctcgcggcgc ctcgtcgtga cgcggcccgg caagtcgcag gccgacgtgg
     5401 cctacgcgat cccgggcgtc gcgcgaacgg ccccggatta cgacgcggtc atgatgatga
     5461 attacgtcct gggcggcggc tcgctctcga gccgtctcat ggatcagatc cgcgatcgcc
     5521 agggcctcgt gtacggcgtc tactccggtc tcatggcggg aatcggcgcc ggcccgctcc
     5581 agattcgcgc ggggacgaat cccgcgaacg cggcgcgctg cgccgacgcg atcctcgagg
     5641 aggtgcggcg tctgcacgac gaggggccga ccgacgagga gatggaggag gccagaggct
     5701 atctcacggg cgtcttcccg gtgcgactcg agacgaatgc cggggtcgcg ggacagctcc
     5761 tgagcgccga gctctacggt ctcggactcg actacgtcga gcgctacccc tcgatcatcc
     5821 gctccgtcac gaccgcgcac gcgcgcgacg ccgcgcggca gcatctggat cccgagcact
     5881 acgtcctcgt gttcgcgggc ccgctcgatc cggccggcct gggagacgtg gagcaggatg
     5941 cgtagactcg ccgtcctcct cgtcctcggc ctcgttctcg cgcttcccag cccggcgcac
     6001 gcgcggagag ttttcgttcc gaagcagcac aagacgatcc agaaggcgat cgaggctgcg
     6061 tcgccaggcg acacggtctg ggtcagcccc ggcgtctatc gcggtcccat cgtgatgaag
     6121 aagccgctca ccctcttcgc cgaccagggc ccggacacga cgatcctgga cggcggcgac
     6181 agcgttcggg tactccagat cgagggagtc aagaatgcgg cggtgatcgg gttcaccatc
     6241 cggcggggcc acgccaactc gggcggcggc atcgcctgcg tggccgacac ggcggtcatg
     6301 atcgcgacct gcctgatcga gaagaactgg gaatcgggca tctcggtgtg gcaatcggtc
     6361 gacatcgaca tgcacgagat ccagttcgtc gagaacacgg ggagcgcgat cgcgctcgac
     6421 ggatcggggg tcgcgatccg cgagtgcctc ttcctcaaga acaagggcta cgccggcggc
     6481 gcgatcacga tgaacaggtc gatcttcatc ttcccggtgc ggacctgccg gttcgaggag
     6541 aacagcgccg agaccggggc cggcggggcc ctgtatgcgg attcgtcgca ggtcctcttc
     6601 gccgagtgca tgttcctgcg caaccactcg ccgctcgccg gaggcgccgt cgcgatcatg
     6661 aacgactcga ggtccgccat ctcccgctgc gtgttccagg agaacgacgc gaaggcggac
     6721 ggcgccatcc atgcggacgg atcgatgctc aacgtgggaa tctgcatctt cgatcggaac
     6781 cggtcgcagg cggcggccag cgcgatcgga tatgtgaagc gtcccccgtc gggactccag
     6841 tcgacgctcg cgaactgcac cttctaccag aacatctcgg gggaatcggc cacgatctgg
     6901 accgagtacg cgtcgccgga gatccggaag aacatctttt ccctcgcgaa gggccagcgg
     6961 gtcacggtcg ccttcaatgg cacgccgctc tacacctgca acctgatcca cgatccgagc
     7021 ggcgcggcgc tcgggtccct tccctcggtc gatacgctcg tcggggaccc gctcttctgc
     7081 gatcccgacc accagaactt caagatccgg gatctctccc ccgcggcgct cgccgcgtgc
     7141 ggcccgatcg gcgcggcgcc gaagggctgc gccagcttca agctggtccc cgccaagtag
     7201 cgaatcgcaa ggccctcgcg gcgattcccc ctcgccgacg gaaccggcaa ccccttccgc
     7261 ctgtagacga atgtgtggcc ggacggtacc ggcccttccg caaaccccaa ggaggtcgaa
     7321 ccatgaggaa cttcagcgcc cgcgtgccgg cggcgggagt cctcgccggg ctggtctgcc
     7381 tgatcctggc gaccccgccc gccttcgctc aacgcgacga cgaggaccag ggcaccccgc
     7441 cgcgggtgcg ggtctaccac gatgacgacg acaacgggcc gtccgcgagt ggcggcggct
     7501 atctgggcgt gcgggtccag gagatcaccc gcgacctgca gcgtgcccgc gatcttccga
     7561 gcacccaggg ggcgctcgtc agcatggtcg cggacggaag cccggctgac caggctggga
     7621 tccgccgtgg cgacgtgatc gtccgggtgg acgggaacga gatcaccgat cccgacgagc
     7681 tgattcgcga gatgaaagac gaggatgccg gtgcccaggt gcgcgtcgtg gtgctccggg
     7741 acggctcccg caggacgatc gatgtgacgc tgggacgccg ccctgcttcg acggacgtgc
     7801 cgcccgccta ccggcggccg ccgaccgggg actacggcga gctacagcgg cgtctgacgg
     7861 ctctggagac ccagcagcgc aggttggagg aggaaatccg ggcgctgcgc gaggaactac
     7921 gcgtccggaa cgggggtcgc gatcgcgacg acgattgacg gctcctgaaa aggcgggcgg
     7981 accgaccggg ggccctggcc caatgacgaa cctcggcacg gccgcccgca cctttcttgc
     8041 atcagactag cagtgcgggg ttggatgggt agaggtggtg tgttacaatg agttccgccg
     8101 gacacctcca cggctgatgt cgcaatttcc gctacatatc ggcctgccca gtccgataac
     8161 cctggagagg gcaagcgttt gccctcgctc cgccttcgca gacagggagt ccacggatga
     8221 gggctaaccg accatgacgc ctatgcttac gatccgccgt gccgcactct cgctcaccgt
     8281 tgcattcctt gtcgaaggag ttctcgtcgg gcccgttccg gcgcaggaag caaccgtcca
     8341 gacggtccct gctggagagg ccttcggcgt gtccgaaaag acacaggtcg ccccgacggc
     8401 gacgacggcg aagcgcaaga ggcatcgcac gcggtaccgg tcgcgccgac gtcacgccca
     8461 ggcgccgctc cggctcccgg cggtcacccc ctacggcatc ccgcggctcc acgtgaaggc
     8521 ggcctacgtc ttcgacgcca cgaacaaccg ggtcctcttc cagaagaatt ccgagcaggt
     8581 cctcccgatc gcgagcctga cgaagctcac gaccgcgatg gttttcctgg gcacgaatcc
     8641 cgactgggac cgcgtgatcg agatggttcc ggaggacatc aagaactcga gccgcacccg
     8701 gatccgcgcg cacgaggaga tcaccgtgcg cgatctcttc catgcctcga tcatgtcgtc
     8761 ggacaacgcc gcgaccaagg cgctcgtccg cacgtgcggg gttccctaca acgaattcat
     8821 cttccgcatg aatctcatgg ccgactcgct cggcctcgcg ggcacgcact tcgtcgagcc
     8881 gacggggctc aacgagcgga acgtctccac ggcgcaggac ctcgcgcgca tcctctggac
     8941 cgccacgcag agcccggtga tccaggccat catgcagaag accgactaca cgttcgtgag
     9001 caaccggaag ctccaccagc tcatcaacac gaaccggctc ctccgcagcc agtggcagat
     9061 cacgggcggc aagaccggct tcatccgtga atcgggctac tgcctggcga cgaaggtgaa
     9121 gtcgcccgag ggctcggaca tcacggcgat cgtgctgggg gctccgagca acgccctgcg
     9181 ctttgccgaa gcccggcgga tcctcgactg gactttccgc ttcggactca cgaagacgga
     9241 gaccaccccg gagagcgagt aggttgccgc cggctcccgg gtgcgttccg atagcccggg
     9301 ggcggctcgt acctgatcgg cccgtgcgga tcacccgcgc gggccttcgt gttgccgcgg
     9361 ccatggccgt gctgacggcc gcagcgctcc acccgccccg ccccgctctc gcggcgccgc
     9421 cgcacagccc tgagtccgac gacgttccca tcgccggcgc cttcgtctac cccgtcggcg
     9481 acgagctcga tttcacgcgc gcccgctccg gcgagtcgca cggctattac gtctccgatc
     9541 cctacatggc gcaccgcgga tccaagaacc agcgcatcca tcagggttgc gacctctcgt
     9601 gcggttcggg tggcgccgcg gtccacgcca tcgcgtcggg tacggtcgtg gtcgcggacg
     9661 cgaacgccct cgtaaagacg cgccgcaagg tggaggtcaa gacaacgaag gtggtgaagg
     9721 ggaagacgat cacgaagacc ggctggaagt ggaagacgtc cacgaagtgg cgcacgggct
     9781 ggggcaacta cgtcgtcctg aagcacgtgc tcccttcggg cgagagcgtc tactcgctct
     9841 acggccacct catgccggcg tccgtgtgcg tggcgcaggg agacatcgtg gcggcgggag
     9901 agacgatcgg tcaggtgggg aagaccggac gcgccagctc gccgcatctc catctcgaga
     9961 tccggaagaa cgccccggcc ggcgacgagg agcggagcga ggacgagacc gagatcgagc
    10021 ccaccgtcca ggatcggacg ttcgcgctcc tcgcgacgac cgatccgatc gacttcctcg
    10081 acaagcacgt ccgccgcttc gaggatctcg agcccggctc ctggcaggcg cgctacgcct
    10141 tcgccgcatg caaggacggc ctcgcggcgc cggacgagaa gcgcttcggg cccgacgagt
    10201 cgatggatcg cggcgagttc taccgctcgc tcgtcctggc cttccggctg agcacggcgt
    10261 tcccggtcgg cgactggacg tccaccgtgt cgtctctggt gcaggcaggc gtgctcgacc
    10321 aggacgggtc ggagggacag aagcccgggg acaagctgac tcaggcggac ggcctcgaga
    10381 tggtgctccg ctgtctcgag cgccatcgtg cgcgcgcgcc caatctcgcc tcgctcgatc
    10441 ccatgcaggt cggtctcgat ttcaatcgac gcttcgccgg agacacggag gccgaggtcg
    10501 ccgcgaagaa gtccaaggtc gccgcgatga aggcgaccga ggcaaagaag aaggccgagg
    10561 ccgatcgcgt ggcgagcgcg cgcaaggcgg ccaaggcggc gggcaagaaa tcccgcgtga
    10621 aggcgcgtac cgtgaagcct gtcgcgccta tgccgatcct cgatccgggc ttcgaagcgc
    10681 tcgcgcagtc gaagaagacc ctcacgcgcg cggagggctg cctcctcctc gcgaccacgc
    10741 tccgcgccgc atcggagcgc atgtccgcgc tccagcgcgc cgccgtccgg gtcgcccagt
    10801 cggagtaggg gcaatgatct tcacccgcgc ggaaggcgtt cacgtctggg accaggacgg
    10861 gcggcgctac ctggacgcga cctccggcgc cttcgccgca cagctcggct actcgcgtcc
    10921 cgatctcgtc cgcgccatga ccgacgcggc gtcgcgcctc ccgcacgcgc ggccgtcgat
    10981 cgcggactcg gaggaggccg cgtcctatcg caaggagctc cttgcccacg ccggcgctcc
    11041 gtacacgcgt gccatcctga cgtcgtcggg gagcgaggcc gtggacgtcg cgctcaagat
    11101 cgcgcatcgg taccaggtcg cgtcgggaca ccccgagcgc catcgcgtcc tctctctcgc
    11161 cggccactat cacgggcaga cgctcgccgc gctcgacgtc acggggtggg ccccgcgccg
    11221 cgagccctac gccgcgcttc taggtcccct gcgcttggga cctgcgccgt actgccgccg
    11281 ctgcttccgc tcgctcacgt ttcccgaatg ccggctcgcc tgcgcggacg ctgccgtgga
    11341 cgagccgccg gcggccgcga tcgtcgagac ggtgcccgcc gcggggctcg gcgcgcccgt
    11401 gcccccgccc ggctggctcg cgcgcctgcg cacgctgtgt cacggcgcgg gtgcgctctg
    11461 gatcgccgac gaggtgctga cgggcttcgg acgcacgggc gatctcttcg cgtggaaacg
    11521 tctcgcccgc cgcgcggccc cgggaggcgg tgcgccggat gccgacgccg ttcccgacct
    11581 cgtcgtcttc ggcaagggcg cgggggccgg ttacgcggcg ctcgccggag tgctcgtggc
    11641 cgaccgcgtc gcgcgaacgg tcgagcctgc gggcttcacc catcaacaaa cgtatggggg
    11701 gcatccggtc gcggccgcgg tggggagggc ggtgctccgc gcgctcgacg aggagggcgt
    11761 gaccgcgcgc gtcgccgagc gggaaccctg gctcgaggag caaatgcgct cgctcggggg
    11821 cgaagcgggc gtgttcgatg ttcgcgggct cggctttctc tggggcgtgg agctggcgca
    11881 ggatgccgcg ggcacgccgt tcccgcggga gaaggcggta tccgagcgcg ttcgggaggc
    11941 gtgccgcgag cgcggacttc tgatccacgc atcgcacgga tgcgccgggg aggaccgcgg
    12001 cgatctcgtt ctcgtggcgc cgccgctcgt gagcgacgac gccgcgctca ccgggatggt
    12061 ggagacgctg cgaggcgcgc tgcgaagcgt cgtcgcgacc gtggaagcct agctacttcc
    12121 ccggacggta cgtgatcacc tgcgcccgct ccagcgtgac gagcccgtcc gccaccatct
    12181 catcgagaac cggcaggaac gcctcgatcc gttccgctcg atccacgatc tcgatcacga
    12241 tgggaaggtc ctcgctcagc cggagcacct tcgccgtgtg gagatggctc ttcgcgccaa
    12301 aacccatgag cccgcggagc acggtcgcgc cggcgagccc ggcctcgcgc gcgcggagga
    12361 cgatcgcctc gtagagcgag cggccgtgcc accggtcggc ctccccgatg aagatgcgga
    12421 ggagctgccc ttcgccttcg agcttcacga tgcgatcctc atggccgcga cgtgcccgcc
    12481 ccagaccgcg acgagcccga gcacggcatg gagcgccaag ttgagtgccc cgcgcccgac
    12541 gtcccccgcg cgcgcgagct cgaacgactc gtacgcgagc gcggagaacg tggtgaaccc
    12601 gccgcagaag ccgatcatga gcgcgctccg ccagagcggt gacgctccgg tgcgctccag
    12661 agtccaggtg gcgatgaagc ccacggcgaa gcaccccagc acgttgaccg ccagggtgcc
    12721 gtatgggaac gttcctcccg aggcgcgctg cacgactcct tgaagcccgt agcgcgccag
    12781 ggtgccgacc aagccgagca cgccgatgag gagcgtctgg agcatgcgcg tactatggac
    12841 cggagggtcg aatcaccgca agccgcacgg ggcaacgcgg atgccgctcg gcccggcgcg
    12901 gggcgcgtgg cctctcgcgg cgctggttac gatcgcggga tgctcgtaca tgtcggacgt
    12961 caagacgagc gctcccggct tgagcgccga cgcgctacgc tcggagaaga tcgcgatcgt
    13021 gagcgtggtc caggtcgacg aggtgaccga cgtgcgtccc cctctcatcg catcgctcga
    13081 gcgcgtgctc cgcgcgaccc ggcccgatct cacgatcgtc ccggcggcga gggtcgcgcc
    13141 ggcgctcgac gactcgaccg agcgattcct gctcctcggc taccagatgc acggggcggc
    13201 cgacccggcg tggctcgagc gtgccaggcc cgagatccgg ccgatggcgc gctacgcgct
    13261 cctcgcgcgt gtcgaggcgg tgaaggtgcg gcactcggac cgtgtcgacg tggcgaccca
    13321 gaccgccggg caggggcttc aggtcaccgg acgggatgtg cgggtgggcg tcagcctcta
    13381 tgatctcgag accctgaagc tcgtgtttgc cggagaatac tggggttcca aggaagaagc
    13441 gatcggcagc cgcgacgcgc ctctcgccga gccggaggac agtctgggca tcgttggggt
    13501 gaacgacacg acgacgtacg gcgtctacaa gaaggccgcg ccggtcgcgc aggccgcgga
    13561 gccggcgttc ctcgagttcg cgcgatcgct gcccggggcg gagaccgcgc gcaccggagc
    13621 gtcggtccga tgaggagcct gaccgcggca gcggcttccg tgatgctcgc ggccgcgctc
    13681 gcgggatgcg gcggcggaca tccaaaaccg gcgcctccgc ccgccgccgc cgtctcggtg
    13741 cggattcctg gaacggagct gcgccagggc gggatcgcga tcatgggcgt ggtcgaggtg
    13801 ggcgaggtgg accaggttcg cccgccgctc gtggccgcgc tcgacagcgt gctcgccgtg
    13861 aagcgccccg agatcccgcg atactcgcag cgccaggtcc ggaacgcggt cgacgattcg
    13921 acagcgcgct tcctcctcct cggctaccag ctccacggcc tcgtggagaa tgcgtggctc
    13981 gatcgtgcag ccgattcgct ccacgacatg gcgcggttcg gaatcctcgc gcgcatcgtg
    14041 cgcacgcgtg tccgccacga cgatgccgag attccggtcc gcccaggctc caaggagacg
    14101 cgcacggtcc gcgcggcgga gctcgacgcc cacgtccagg tgcacgtcta cgaccttcgc
    14161 gcccggacgc ttcgcgcgag cggggagttc ctggggatcg gggagcatcg cgctcgtccg
    14221 gacagcgtgg ccgagctccc gaccatccga acctgggtgt cgccggagca gcggacgcag
    14281 tcgatggtgc cgccgcccgg cgggtacttc gagatggatc cgccgcccct ctcggtggcc
    14341 gtcggacccg cgtttgccgc ggcgatcgat gcgctcttcg gaccgccgga cacgacaggc
    14401 gcacggaccg actccacgcg ccgttcgaac cgctagctcg gatcgatcgc gacgcgtgcg
    14461 gcgcgtcggg cttcgcggcg ctccatccag ggcttccaga ggatcgtcac ggcaaccccc
    14521 agaaggatga tggccgcgcc caggacctcg cgcgtcgaca tcccttctcc gaagaaggcc
    14581 accgcgaaga gcgcggcgaa gatcggctcc gtggcaaaga tcatcgaggc gcgcacgggg
    14641 gtcgtgcgcg cgagcgccca cgactggagc gcgatcacga ccgcggtcgc gacgacagcg
    14701 aggtagacga gggtcgccca gggaacctgc gaccacggcg tgtggaagcc cccgcgcgcg
    14761 atgacgatgc cccaccccgc gagcgctccg acacccatct gcaccacgct cagtgccacg
    14821 ggatccacct tcttccccgc gacgttcgtg agcacgatct ggatcccgaa cgacagggcg
    14881 caggcgagcg tccagagatc cccggcgcgg agggagaacc cggcgtctgc cgagaagagc
    14941 gtgaggcccg cgaatccgat caggatgccg aggatctcgc ccaccctcgg gacttccccc
    15001 gccatcggga tcgcgaggaa cggcgtgtag aggatgtagg tgcacgtcag gaaggccgag
    15061 cgcgaggccg tgatcgtcgc gagcccgatg atctgcgtga gatatcccga tgcgagaaag
    15121 attccgatcc agccgccgcg gaggagcgct tccttcttga gcgggaacac tcggcgcggg
    15181 aagaccgcga gcaggatgac cgtcgccaac gtgaaccgga gcgccatgag ctccgcgggg
    15241 tggatcgagc ggagcgaatc gcgaagcagc gcgaacgtcg tcccccagat cgcgcaaacg
    15301 aggaggagga cgaggtcggc cgtgctcacg ccgtcacgcc gtcttgcggc gccggccgtt
    15361 cgtcgtcgct ggcttcgggt ggatcccctt gatggagaaa gcgaggctct cgagctcgat
    15421 cgagagattc acggtgcgaa gggccacggt cggcggcacc tgaatgcggc ggtgggcgaa
    15481 gttcaggatc ccgcgcattc cggccgcaac gaggcgatcg gcgacggcct gcgcggcgcg
    15541 cgccggaacg gcgatcacgc cgatctcgag cccgagccgc tgggcttccg tctcgagctc
    15601 gtcgatgtgg tggatcgcga ggtcgcgcca cttctgtccg acgcgctcgg cgctcatgtc
    15661 gaagacgccg acgatgtcga atccctggcg gcggaattcc ttgtaggagt agagcgcgga
    15721 gccgaggttc cccgccccca cgagcgccac cttccagcgg cggttcaagc cgaggatgag
    15781 cctgatctcg cggcggaggc ggggcacgtt gtagccgagc cctcgctttc cgaaattgcc
    15841 gaagtaggag agatccttgc ggacctgggc ggacgtgacg ccgctctggt gcgccaggcc
    15901 ctgggaggag atcgtgcggt gcccttcggt ctccagctcc tcgaggaccc ggtagtaccc
    15961 ggacaggcgc ctgatggtcg acgtcggaat ccgcggatcc aactacgctt ctccgtcttc
    16021 ctccgactcc tcgacgcgcg cgacgtcgat gagctggtcc cccggctcga gcccgatgag
    16081 ccggacccct tgcgtgttcc ggccgatctc ggagatgccc gacacctgca tgcggatgac
    16141 gatcccgccg cgggtgatga gcatgacctc gtcgtccccc accacttcga gcgcggccac
    16201 gacgctgccg ttccgctccg tggtctggat ggagatgatc cccttcccac cgcggcccgt
    16261 gatccggtat tccgagatcg gcgaccgctt gccgtagccg tgctcggtcg cgaccaggat
    16321 gctcgcctcg cggcgcaccg cgatcacgcc caccacggcg tcgttttcct cgagcgtcac
    16381 gccccgcacg ccatacgccg tgcgtcccat cgggcggacg tccgtctcct tgaagcggat
    16441 ggccttgccg ttccgcttgg acaggatgat gtcctgcgtg ccgtccgtga ccacggcgtc
    16501 gatgagcgcg tcctcgggct cgacgccgat cgcgacgatg ccgccgcggc gcggattgga
    16561 gaaggccgag agcggagtct tcttcaccgt cccgccgcgc gtgaagagga agatgtagcg
    16621 gtcctcctgg aagcccttca ccggcacgac cgcggccacg cgctcggtct gggacatctc
    16681 gaccacgttg acgatggcct tccccttcgc ggtgcgcccc ccttgcggga tctcatggac
    16741 cttgagccag tagacgcgcc cgcggtcggt gtagaccagg atgtagctgt gggtggacgc
    16801 gatgaagagg tgctcgatga agtcctcctc gcgcgtgccc gccccggtga tgccgcgccc
    16861 gccgcgtcgc tggctccggt acgtcgtcac cgggagccgc ttgatgtagc ccgcgtgcga
    16921 gatcgtgatc accatgtcct cctcggcgat cagatcctcg atctcgaact cgcccgtggc
    16981 ggcgacgatc tcggtccggc ggtcgtcgcc gaagcgctcc ttgatctcgg ccaggtcgtc
    17041 ccgaatgatc tgaagcacct tcgccggcga gtcgaggatg gctcggagcc gctcgatgag
    17101 ctggatcacc tcgaggtact cgtcctcgat cttctggcgc tcgagccccg tgagtcggga
    17161 gagccgcatc tcgaggatgg cgttcgcctg gagctcactc agcgcgaagg tcgacatgag
    17221 cccttggcgc gccgcctcga cgttggccga ggccttgatc agcgccacga tctcgtcgat
    17281 gtggtcgagg gcgatcttga agccctcgag gatgtgcgcg cgccgctccg cctcgccaag
    17341 gtcgaacttg gtccgccgca ccaccacttc ctgccggtgc gcgatgtagt gctggatcat
    17401 ctccttcagc gtgagcgtca gcggccggtt gccgacgagc gccaagaggt tcgatccgaa
    17461 ggtcgtctgc atctgcgtgt gcttgaagag ctggttcagg atcaccttgg gctgcgcgtc
    17521 ccgcttcagc tcgatcacga cccgcatgcc ctcgcggtcg gactcgtccc tgaggtccga
    17581 gatgccgtcg agcacgccgt ccttcacgag gtcggcgatc ttctcgagga gggccgactt
    17641 gctcaccatg taggggatct cggtgacgat gatcgcctgg cggtccttct tcatctcctc
    17701 gatcgtggcg agcgatcgga ccgtgatgtg cccgcggccc gtcgtgtagg agtcgatgat
    17761 cccctggcgg ccgtagatga cgccgccggt cgggaagtcg ggcccttgca cgatgcggag
    17821 gagatcctcg atcgggcagt ccgggttctc gatcacgtga tggatcgcgt ccacgatctc
    17881 gcgaaggttg tgcggcggga cgttggtcgc catgccgacc gcgatccccg aggagccgtt
    17941 gacgaggagg ttcggcaccg ccgccggcag caccaccggc tcctcgcgcg tctcgtcgta
    18001 gttcggccgc atgtcgaccg tgtccttctc gatgtctcgg agcatctcct cggcgaactc
    18061 ggtgagcctg gcctccgtgt atcgctcggc cgccggggcg tcgccgtcga cggagccgaa
    18121 gtttccctgg ccgtcgacga gcgggtagcg catggagaag gactgggcga ggcggaccat
    18181 cgtctcgtag accgggatgg tcccgtgcgg atggtagttg ccggtgacgt cgcccgtgat
    18241 cttggcggac ttacggaagg gccggtcgtg gaggaggttc agctcccgca tcgcgaccag
    18301 cacgcgccga tggaccggct tcagcccgtc gcgcacgtcc gggagggcgc gcgacacgat
    18361 gaccgacatc gagtagtcga tgtaggaatt ccgcatctcg tcttcgacgt agatcggtat
    18421 gactttgtca cgcgtcgcca tgcggcgtgg ttcctttctc tagttcctag ggatcagacg
    18481 tccagattcc ggaccctgtc cgcgaattcc tcgatgaact tccggcgcgg ctcgacctga
    18541 tcccccatca agatcgtgaa catgcggtcg gcctcgacca ggtcgtcgag cgtcaccttg
    18601 aggacgctcc gcgactccgg atccatggtc gtcgcccaga gctggtcggg attcatctcg
    18661 ccgagcccct tgtaccgctg gaggtggacg cctttccggc cgatgtcatc gagcgccgcg
    18721 tcgcgctcgg catcgttgta ggcgtagcgg acgtccttcc ccttggccac tcggtacaga
    18781 ggaggctgcg cgatgtagat gtagccgcgc tcgatcagtg gccgcatgtg gcggaagaag
    18841 aacgtgagga ggagcgtccg gatgtgcgcg ccgtccacgt ccgcgtcggt catgatgatg
    18901 atccggtgat agcgcgcctt gtcggcgtcg aactcgtcgt cgatgccggc cccgagcgcg
    18961 gtgatgatcg tgcggatctc ctcgttcgag agcatcttgt cgagtgaggc tttctcaacg
    19021 ttcaggatct tcccctttag gggaaggatc gcctggtagc gccggtcgcg ccccatcttg
    19081 gccgagccgc cggccgagtc cccctcgacc aggtagatct cgcactgcgc gggatcggtg
    19141 agctggcaat cggcgagctt gccggggagc gagcccgagt cgaggatgct cttccgccgc
    19201 gccagatcgc gcgccttcct cgcggcctcg cgggcgcggg cggtggcgat gcacttctcg
    19261 atgatcttct tcgcgatctg gggctgctcc tcgaagcagg tgcgcatccc ctcgcccacg
    19321 accgactcga cgatcccctt cacctcggtg ttgccgagct tggccttggt ctgcccctcg
    19381 aactgaggct cggggagctt cacgctgatg accgcggtga ggccctcgcg cacgtcgtcc
    19441 ccgccgagcg agagatcttt caggttcttc agcatcccct cgcgctcggc gtagttgttg
    19501 atggtgcgcg tgagcgccgc gcggaagccg atcagatgtg tgccgccttc taccgtgttg
    19561 atgttgttca cgaaggagaa gacgttctcc acgtagccgt cgttgtactg cagcgcgaac
    19621 tcgacgatca ccttgtcccg ctcgcgcgag tgggagatgg gcgacgggtg gaggacggtc
    19681 ttgttctcgt tcaggtactt cacgaaggag acgatgcccc cctcgtactg gaagtcgtgc
    19741 ttcttccccg tccgctcgtc caggaactgg atccggatgc ccttgttgag gaacgcgagc
    19801 tcgcggagcc gctgcgagag ggtgtcgaag ttgtaatcga ccgtctcgaa gacgttgggg
    19861 tcgggcttcc agtggacggt cgtgccggtg cggtcggtcg gccccagctc ctcgacgtcg
    19921 cttgcgacca cgccgcgctc gtagcgctgg cgatagcgct tcccggcgcg atagacgtcg
    19981 acttcgagcc actcggagag cgcgttcacc accgacacgc ccaccccatg gagtccgccc
    20041 gagaccttgt acgtgttgtc gtcgaacttc ccgcccgcgt ggagcgtcgt catcacgacc
    20101 tcgagcgcgg gacggttctg cgacggatgc atatcgacgg gaatgccgcg gccgtcgtcc
    20161 accacggtca cactcgtgtc ggggtggatg gtgaccagga cctcggtgca gaacccggcg
    20221 agcgcctcgt ccaccgagtt gtcgaccacc tcgtagacga gatggtggag gccgccctgc
    20281 ccggtcgacc cgatgtacat ggcgggacgc ttgcgcacgc cctccaggcc cttcaggacc
    20341 tggatgttcc gggcgtcgta cgagtgcccg ccttcctggg ccgtcgtcag agtccctccc
    20401 tcatcgcgcg tctggttcaa gtgcgccttc ctccctgctc cgcatcggcg tgagccggat
    20461 ggcccggagc ctggcttcga tcggaagccg tgcgttgatg tcgtccatga tctgctgtct
    20521 cagcaccgcg aggtgcccca tccacaccga gcccctcact tccacgagga gctc
//
LOCUS       DASTKC010000010         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_246033, whole genome shotgun sequence.
ACCESSION   DASTKC010000010 DASTKC010000000
VERSION     DASTKC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246033"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..954)
                     /locus_tag="VFR25_00435"
     CDS             complement(25..954)
                     /locus_tag="VFR25_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET9939544.1"
                     /translation="MDFTSTIAAMARVFGDAAIEILPLFLLALALAAVLEEFVSNRTI
                     ERFLTGRHPATMLVASTTGALIPLCTCGMVPLAVSLRRRGSDLKHTFAFLTAGAAVSV
                     PVLLLTWKVLGFGWMVVRFVTSVIFGLGVGYAAVRALRNEAARSAESPGGRTAGALGP
                     APDVDEDVTPIRRSRWVTLLRRLRGQLLEYFPWVLVSLALAAVVDVLVPRHVVSLLYG
                     QKSVIGSVAAALTGVPFYFCSGAELPLAGELLSKGMGHGPGVAMLLSVPIVNVLTFGV
                     VGRWLGPRGAAIYLGLCVLGAAVIGTITGWIWA"
     gene            1044..3554
                     /locus_tag="VFR25_00440"
     CDS             1044..3554
                     /locus_tag="VFR25_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9939545.1"
                     /translation="MHDPFYHPIFLGRRAALETPPFDPFPPSGRKPWTESPSPRRDLE
                     FHVRHYKVELDVDFEKKELSGRTSLTIESLRDGLRDVVLDAAEMRIASVKIGRRSLAY
                     ASEGEKLRIALGEPLRAGSHAVIEIEYATRPRKGFFFVGPTEAEPNRVPSGWSQGQAN
                     DTHGWIPCLESTESRGTLDMVVTVPAGYRAIGNGRLVSRKARRAAAPARRNGRRRGKN
                     GRVKQGTVTYHWRQDTAHPVYLTSLVVGKYSELKDRAGSVPLFGYVPPGLEREGRDLF
                     RKTPAMIATFQRVFGFPYPYPKYAQSTVADFTWGGMENTSATTLMERVLHAGNDSFEE
                     RYDSLIAHELAHQWWGDLVTCRDWSEGWLNEGFATYSEIVFRESDEGRDDADYARLEQ
                     MSSYLTEDGEDYRRPLVETRWNYPSSLFDRHLYEKGACVLHMLRALVGDAAWRRSLKR
                     YLERHAFGSVETADLRRAFEEETGRNLSWFFDQWVYHGGHPELRVTRSWDEGARTLLL
                     TVEQVQEVDEVTPLFRIPLTLEVVSAGRRLRIPLDLNGKRETIHVPLPGRPRYVALDP
                     EHDVLKTMDFARSDEELLFGLRRSPFALERIRCARELSRRGDERVIGALMKTLRGDRF
                     WGVRAAAAVSLGEIGRRVPGLSSRLAREADQTSTRVRRGVLWALGWLGDDAALKRLTR
                     SVAEETSSFNIGLALIGIARAKGEGAFEALKAELKRESHRDMLRVLIFDAMTRLRDAR
                     AIPILLEHTQPRFRNEAREAATKAFGKLGVLNPEVERRLEELTQDSWFRVRSAAAGSL
                     RRLKSPRAEAAIREALRTEPLDGVRSALEKALTDGHSG"
     gene            3619..>4417
                     /locus_tag="VFR25_00445"
     CDS             3619..>4417
                     /locus_tag="VFR25_00445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9939546.1"
                     /translation="MPLAQGIKLGPYEILTPLGSGGMGEVYRARDSRLGRDVAVKVLP
                     HSFAKDRERLQRFEHEARAAGALSHPNVVAVFDVGATDGIPYLVSELLEGESLGNLIR
                     RGPLQPRRAIELMIQAASGLAAAHAKGIVHRDLKPENLFVLPDGRLKILDFGLAKLLR
                     DDAPPRDETGPQLHTLTQTGQIMGTASYMAPEQIRDQPVDHRADLFALGAILHELITG
                     TKAFPGETPADRMTAILTKDAAEWDGAVDLEAPGLALIARRCLEKRAD"
BASE COUNT          712 a         1555 c         1525 g          625 t
ORIGIN      
        1 cggtggtcac gtcgagccga aggcctaggc ccagatccac cccgtgatcg tcccgatcac
       61 ggccgcaccc agcacgcaga gtccgaggta gatcgcggcg ccgcgcggtc cgagccaccg
      121 tcccaccacg ccgaacgtca gcacgttcac gatcggaacc gacagcagca tcgccacgcc
      181 cggtccatgc cccatgcctt tggagaggag ctcgccggcc aggggcagct cggctcccga
      241 gcagaagtag aagggcacac ccgtgagcgc cgcggcgacg gatccgatca cgctcttctg
      301 tccgtacagc aggctgacca cgtggcgcgg cacgagcacg tcgacgaccg ccgcgagcgc
      361 gaggctgacg aggacccagg ggaagtactc gagcagctgc ccgcgaagtc ggcggagcag
      421 ggtgacccag cgcgagcggc ggatcggggt cacgtcctcg tccacgtccg gcgcgggacc
      481 cagggcgccc gcggttcgcc cgccggggct ctccgcggaa cgagccgcct cgttccggag
      541 cgcccgcacc gccgcgtagc cgaccccgag cccgaagatc accgacgtca cgaagcgcac
      601 caccatccac ccgaagccga gcaccttcca cgtgagcaga agcaccggaa cgctcacggc
      661 cgcccccgcc gtcaggaagg cgaacgtgtg cttgaggtcg ctcccgcggc ggcggagcga
      721 cacggccagc ggcaccatgc cgcaggtgca gagcggaatc aaagcgccgg tcgtggatgc
      781 gaccagcatc gtcgccgggt ggcggccggt caggaagcgc tcgatcgtcc gattggacac
      841 gaactcctcc aggaccgccg ccagcgcgag ggccaggagg aataggggaa ggatctcgat
      901 ggccgcgtcg ccgaagacgc gcgccatggc ggcgatcgtg gaggtgaagt ccatgagccc
      961 attgtatccc gcgcggcccg ccttgacacg cgcgcgcggc tcgggggacc atggcttgac
     1021 tcgaccgaaa ggagccattg ccggtgcacg acccgttcta ccatccgatt ttcctcggcc
     1081 gccgtgcggc gctcgagacg ccgcccttcg acccgttccc tccctcgggg cggaagccgt
     1141 ggaccgaatc gccctcgccc cgccgcgacc tcgaattcca cgtgcggcac tacaaggtcg
     1201 agctggacgt cgacttcgag aagaaggagc tcagcggccg cacctcgctc acgatcgaat
     1261 cgctccggga cggccttcgc gatgtggtgc tcgacgcggc cgagatgcgg atcgcgtcgg
     1321 ttaagatcgg ccgccgctcc ctcgcgtatg cgagcgaggg cgagaaactc cgcatcgcgc
     1381 tcggcgagcc gcttcgcgcg gggagccacg cggtgatcga gatcgagtac gcgacccggc
     1441 cgcggaaggg gttcttcttc gtcggcccga ccgaagcgga gcccaaccgg gtgcccagcg
     1501 ggtggtcgca ggggcaggcg aacgacaccc acggctggat tccgtgcctc gagagcacgg
     1561 agagccgcgg cacgctcgat atggtcgtga cggtgccggc cggctaccgc gccatcggca
     1621 acggccggct cgtgtcgcgc aaggcgcgtc gggccgccgc accagcgcgg cgaaacggcc
     1681 gccggcgcgg gaagaacggc cgcgtcaagc agggcacggt cacctatcac tggcgccagg
     1741 acacggcgca ccccgtctat ctcacgtcgc tcgtcgtcgg caagtactcg gagctcaagg
     1801 atcgcgccgg atcggttcct ctcttcggct acgtgccgcc ggggctcgag cgcgaggggc
     1861 gtgatctctt ccggaagact ccggcgatga tcgcgacctt ccagcgcgtg ttcggcttcc
     1921 cgtatccgta tcccaagtac gcgcagagca cggtcgccga cttcacgtgg ggcggcatgg
     1981 agaacacgag cgccacgacg ctcatggagc gcgtgctcca cgctgggaac gactccttcg
     2041 aggagaggta cgactcgctc atcgcgcacg agctggccca ccagtggtgg ggcgatctcg
     2101 tcacgtgccg cgattggtcc gaaggctggc tcaacgaggg cttcgccacc tattccgaga
     2161 tcgtgttccg cgaatcggac gaggggcgcg acgacgccga ctacgcgagg ctcgagcaga
     2221 tgagctcgta cctcacggag gacggcgagg actaccgccg cccgctcgtc gagacgcgat
     2281 ggaactaccc gtcgtcgctc ttcgaccggc acctctacga gaagggcgcc tgcgtgctcc
     2341 acatgctccg cgccctcgtg ggtgacgcgg cctggcgccg ttcgctcaaa cgctacctgg
     2401 agcgacacgc cttcggatcg gtggagaccg cggatctgcg ccgcgcgttc gaggaggaga
     2461 cggggcggaa tctctcctgg ttcttcgacc agtgggtcta tcacggcggc catcccgagc
     2521 tccgggtcac gcgctcgtgg gacgaaggcg cgcgcacgct cctcctcacg gtggagcagg
     2581 tgcaggaggt ggacgaggtc acgcccctct tccgcattcc gctgacgctc gaggtggtga
     2641 gcgcggggag acggctccgc atcccgctcg atctcaacgg caagcgcgag acgatccacg
     2701 tgccgcttcc ggggcgtccg cgttacgtcg cgctcgatcc cgagcacgac gtgctcaaga
     2761 ccatggattt cgcgcggtcg gacgaggagc tcctgttcgg tctccgccgc tctcccttcg
     2821 ccctcgagcg gatccgctgc gctcgcgagc tgtcgaggcg gggagacgag cgcgtgatcg
     2881 gcgccctcat gaagacgctg cggggggatc gcttctgggg ggtgcgcgct gccgccgcgg
     2941 tgtcgctcgg ggagatcggc cgccgcgttc cggggttgag ctcgcggctc gcccgcgagg
     3001 ccgatcagac gagcacgcgc gttcggcgcg gcgtcctctg ggcgctcggc tggctcggcg
     3061 acgatgccgc gctcaagcgc ctgacgcggt cggttgcgga ggagacctct tcgttcaaca
     3121 tcgggctcgc gctcatcggc atcgcgcgcg caaaggggga gggcgcgttc gaggcgttga
     3181 aggccgagct caagcgcgag agccaccgcg acatgctgcg cgttctcatc ttcgacgcca
     3241 tgacgcggct ccgcgatgcg cgagcgattc cgatcctgct cgagcacacg cagccccggt
     3301 tccggaacga ggcgcgcgag gccgcgacca aggccttcgg aaagctcggg gtcctgaacc
     3361 ccgaggtgga gcgccgcctc gaggagctga cccaggattc gtggttccgc gtgcggagcg
     3421 cggccgccgg ctcgctccgc aggctcaagt cgccgcgcgc cgaagcggcc attcgcgaag
     3481 cactccgcac ggagcccctg gacggcgtcc gctccgccct cgagaaagcc ctcacggacg
     3541 gtcattcggg ctgacctccg ccgcgcgcgt ggggtggtac actgcatgtg aacgaccatc
     3601 ccgcgtcccc ggagaacgat gccgctcgcc caaggaatca agctcggccc ctatgaaatc
     3661 ctgacgccgc tcgggtcggg aggcatgggc gaggtctacc gcgcgcgcga ctccaggctc
     3721 ggccgggacg tcgctgtgaa ggtgctcccg cattcgttcg cgaaggatcg cgagcggctc
     3781 cagcgcttcg agcacgaggc gcgcgccgcc ggcgcgttga gccatcccaa cgtggtcgcc
     3841 gtgttcgacg tcggcgccac cgacggcatc ccgtacctcg tgagcgagct cctcgaagga
     3901 gagagcctgg gcaacctgat ccgccggggc ccgctccagc ctcgccgcgc gatcgagctc
     3961 atgatccagg cggcgtcggg gctggccgcg gcgcacgcga agggcatcgt ccaccgcgat
     4021 ctcaagcccg agaacctctt cgtcctcccg gatgggcgcc tcaagatcct cgatttcggc
     4081 ctcgccaagc tcctgcgcga cgacgcgccg ccgcgggatg agacgggtcc tcagctccac
     4141 acgctcacgc agaccggcca gatcatgggc acggcgtcct acatggcccc ggagcagatt
     4201 cgcgatcagc cggtggacca tcgcgcggat ctcttcgcgc tcggcgcgat cctccacgag
     4261 ctgatcacgg ggacgaaagc gttcccgggg gagacgcccg cggatcgaat gaccgcgatt
     4321 ctcaccaagg acgcggcgga gtgggatggg gccgtggacc tcgaggcgcc cggccttgcg
     4381 ctcatcgcgc ggcgctgtct cgagaagcgg gcggacc
//
LOCUS       DASTKC010000011         8574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_956540, whole genome shotgun sequence.
ACCESSION   DASTKC010000011 DASTKC010000000
VERSION     DASTKC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956540"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFR25_00450"
     CDS             complement(<1..243)
                     /locus_tag="VFR25_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9939547.1"
                     /translation="MITPELLAQVRRLTIRSRRAVEEVFSGAYRSAFRGQGLEFAEVR
                     EYVPGDDIRAIDWNVTARVGHPFVKRFDEERELTVVV"
     gene            complement(240..1229)
                     /locus_tag="VFR25_00455"
     CDS             complement(240..1229)
                     /locus_tag="VFR25_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9939548.1"
                     /translation="MTEATLEERTARVREESAFIAPLLREIERTIVGQDEMVRRILIG
                     LLADGHLLLEGVPGLAKTLAVKTVAQTIHATFHRVQFTPDLLPADLTGTLVFDPKTGA
                     FTPKLGPVFTQILLADEVNRAPAKVQSALLEAMQERQVTIGEKTHPLGDLFWVLATQN
                     PIEQEGTYPLPEAQMDRFLLKIRVGYPSPEEEGRILDRMGGDPPEPPKRVIDVERIIK
                     SRHTANGIYLDERIRRYIVDLVHATREPERFGLDLRPLIQYGASPRATLALARSARAN
                     AFLDGRAYVTPDDIKALAPDVFRHRILLTYEAEAEGVPVDEVSRRLLARVEVP"
     gene            complement(1252..2535)
                     /gene="glyA"
                     /locus_tag="VFR25_00460"
     CDS             complement(1252..2535)
                     /gene="glyA"
                     /locus_tag="VFR25_00460"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9939549.1"
                     /translation="MARSEAAPVSAHDVALHAADPEIARAIDDEVHRQQTTLELIASE
                     NFVSEAVLEAMGSPLTNKYAEGYPGKRYYGGCEFVDKAESLAIERAKALFGAEHANVQ
                     PHAGAQANLGTYLALMPPGSTLLGMALAHGGHLTHGHKVSHSGIIFRSVQYGLSPDTG
                     LLDYDQVAAMAREHKPRVIVAGASAYPRFFDYAKFRAIADEVGAAFVFDMAHVAGLVA
                     GGVHPSPVPYADVVTSTAHKTLRGPRSGFILCKSQFAKAIDKSVFPGMQGGPLMHVIA
                     AKAVCFREASTPAFKEYAAQVVANAKTLAQELITHGYELVTGGTDNHLILLDLRNAGF
                     SGAEAEEALHKAGITVNKNAVPNDPRPPAVTSGIRIGAAAVTTRGLGEPEFRTLGRWI
                     DEALQKRSEPAALAKIRAQVEDLCSSFPLYPNRAP"
     gene            complement(2558..3520)
                     /locus_tag="VFR25_00465"
     CDS             complement(2558..3520)
                     /locus_tag="VFR25_00465"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9939550.1"
                     /translation="MPEEVRGTIWMNGKFVPFEEATIHVLSHVVHYGSSAFEGIRAYD
                     TPHGTAIYRLDRHVQRLLDSCKIARLDIPYLYDELMEATRETVRRNGGKACYIRPVVY
                     RGFKTLGVNPTGVPVDVAIATLNWGKYLGKDALERGIAVKVSSWRRAAPDTFPALAKT
                     GANYMNSQLVKLEAITDGYQEGIALDSFGYVAEGSGENIFLVRNGVLHTPTIANAILP
                     GITRDSIIQIAKDLGIEVREEQIPREALYIADEVFFTGTAAEITPITSIDRIGVGSGT
                     VGPMTRRLQDAFFDIIEGRARDRHGWLFPVDAKTKVQRAAKKAR"
     gene            complement(3553..4044)
                     /locus_tag="VFR25_00470"
     CDS             complement(3553..4044)
                     /locus_tag="VFR25_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939551.1"
                     /translation="MIQVLIVCTGNTCRSPMAEGALRSLLPSEIAAQVRVRSAGTNAV
                     PGVPATPLAVSTTGSRDIDLRGHQSTSLTPELIRESELILGMEPGHVTRARELAPDAA
                     DRIHLITEQGADGAEAMRTGIHDPIGGTSVEYDDTFNRIRSHLLRWLPLIREAVERRE
                     GVR"
     gene            complement(4087..4713)
                     /locus_tag="VFR25_00475"
     CDS             complement(4087..4713)
                     /locus_tag="VFR25_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET9939552.1"
                     /translation="MSAVRLAIGAHPDADLIARAAAALLEDKVALLPAEGVYGFHARA
                     SSAIAIERIRALKRDPRAGFIGLIAEPGDLERWAHASPHALELARRYWPGALTLVLDA
                     RPEAPESLRSESGTIALRCPGSDLLRGVVQGVGEPVLSTSANRSGEPPALRADDAPSG
                     VADLIVDGGVLDGRPSTLVRVGPKGVEILRQGAVRLGDGSLDGLEAEP"
     gene            complement(4718..5194)
                     /gene="purE"
                     /locus_tag="VFR25_00480"
     CDS             complement(4718..5194)
                     /gene="purE"
                     /locus_tag="VFR25_00480"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9939553.1"
                     /translation="MSTKTRVAILFGSESDRTTMQETAKVLDSFGIGYEMEQASAHRS
                     PDKVRDIVRGAPGRGVEVFVAAAGMANHLAGAVAAHTSLPVIGVPLGGSPLGGADALL
                     STVQMPAGVPVATVAIGSAGAKNAGVLAAQILAISDPALRTKLEDLKARLARGEKL"
     gene            complement(5191..6492)
                     /gene="purD"
                     /locus_tag="VFR25_00485"
     CDS             complement(5191..6492)
                     /gene="purD"
                     /locus_tag="VFR25_00485"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET9939554.1"
                     /translation="MSEPRVLVVGQGGREHALVDALHRSPDHPKIFVAPGSPGMEPLA
                     ERVAIEAGDVPSLVGWVNSHAIDLVVVGPEIPLERGLADALREAHVPVLGPGRDGARL
                     ETSKVFAKELFQKAGLPSASFQVARSAEEAERMVDAGPLPIVLKADGLAAGKGVVVAM
                     SKDEAMAAIDGWMRRGMLGDSGRTLVIEEFLEGEEASILLLTDGERWVLFPPARDHKR
                     LRDNDTGPNTGGMGACSPARVPAPEDAKAIAHALVDPLLAALRAEGTPYRGILYLGLM
                     LTAQGPQILEVNARFGDPEAQAVLPIVSEDLLPLADQAARGRLPEARHGTFVAHEGAA
                     VCVVLAARGYPASPESGAVIEGLNGPWPHGICVYHAGVASRGGQWITSGGRVLGVTAR
                     SETLERARLAAYQAVGRIRFDGMQYRTDIAQSPQTSGASRP"
     gene            complement(6489..7964)
                     /gene="gatB"
                     /locus_tag="VFR25_00490"
     CDS             complement(6489..7964)
                     /gene="gatB"
                     /locus_tag="VFR25_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET9939555.1"
                     /translation="MSPAATATARERYEPVIGLEVHAQLKTASKIFCGCSTRFGAPPN
                     TQVCPVCLGLPGVLPVLNERAVRYAVMVGLAVGSKISTRSVFARKNYFYPDLPKGYQI
                     SQYDRPLCEGGSLEIRSGEGWKRIGLVRIHLEEDAGKSLHPEGKEGVHTTRVDLNRAG
                     VPLIEIVSEPDIRSGEEATDYMARLRQILVYLDVCDGNMEEGSLRCDANVSVRPKGAS
                     TFGTKTEIKNLNSFRFVDRAIAFEIERQIGLIESGGRVVQETLLWNSERSVAESMRSK
                     EEAHDYRYFPEPDLLPLDLDPAWIESVRTSLPELPHALWERLVREYEIPEYDAEVLTE
                     TRSLATYYEEVVRLSKRPKLASNWIMTEVNAIRNRDGLSIETFPIQADRLGALVKMIG
                     DATISGKIGKTVFEHMLTDPSSPEEIVKKHGLLPIDDEESLLKLAEEIVAANPGPAAE
                     FRAGKEKTFGFFVGQAMKRSGGRAHPEKLQAALKAALAKQG"
     gene            complement(7970..>8574)
                     /locus_tag="VFR25_00495"
     CDS             complement(7970..>8574)
                     /locus_tag="VFR25_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9939556.1"
                     /translation="GAGQAEVDLPHAKYAIATYYLIAPAEASSNLARYDGVRYGLRAG
                     NGDLQGMYETTRGLGFGREVTRRILLGTYALSAGYYDAYYLRAQKVRTLIRRDFTEAF
                     AKADAILLPVTPTPPFKLGEKADDPLAMYLNDIYSIPASLAGIPAMSFPAGFTKSGLP
                     IGLQLVGKPFDETTLFRIARTWEKASDEAWRIPPIAEEAA"
BASE COUNT         1262 a         2913 c         2991 g         1408 t
ORIGIN      
        1 cacgaccacc gtgagctcgc gctcctcgtc gaagcgcttc acgaagggat gccccacgcg
       61 ggccgtgacg ttccagtcga tcgcgcggat gtcgtcgccg gggacgtact cgcggacctc
      121 ggcgaactcg agcccctggc cgcgaaacgc cgagcggtag gcccccgaga agacttcctc
      181 caccgcccgc cggctccgga tggtgagcct ccggacctgc gcgagcagct cgggggtgat
      241 cacggaacct cgacgcgggc gagcagtcgc cgagagacct cgtcgaccgg gaccccttcg
      301 gcctcggctt cgtacgtgag gaggatccgg tggcggaaga cgtcaggggc gagcgccttg
      361 atgtcgtccg gcgtgacgta cgcgcggccg tcgagaaacg cgttggcccg cgccgagcgc
      421 gcgagcgcga gggtcgcgcg cggcgacgcg ccgtactgga tgagcggcct gagatcgagc
      481 ccgaaccgct cgggttcgcg cgtggcgtgg acgaggtcca cgatgtagcg gcggatccgc
      541 tcgtccaggt agatcccgtt cgccgtgtgc cgcgacttga tgatgcgctc cacgtcgatc
      601 acgcgcttcg gcggctcggg cggatcgccg cccatgcggt cgagaatgcg cccttcttcc
      661 tcgggactcg ggtagccgac ccggatcttg aggaggaagc ggtccatctg cgcctcggga
      721 agcggatacg tcccttcctg ctcgatgggg ttctgcgtcg cgagcaccca gaagagatcc
      781 ccgagcggat gcgtcttctc gccgatcgtg acctgccgct cctgcatggc ctcgaggagc
      841 gcgctctgca ctttcgcggg cgcgcggttg acctcgtcgg caaggaggat ctgcgtgaac
      901 acgggcccca gcttcggcgt gaacgcgccc gtcttcggat cgaacacgag cgtgccggtg
      961 aggtccgccg ggaggagatc gggcgtgaac tgaacgcgat gaaacgtcgc atggatcgtc
     1021 tgcgcgaccg tcttcacggc gagggtcttc gccagaccgg ggacgccttc gaggagaagg
     1081 tgcccgtcgg cgaggagtcc gatcaggatc cgccggacca tctcatcctg cccgacgatg
     1141 gtccgctcga tctcacgtag gagcggtgcg atgaacgcgc tctcctcgcg gacgcgcgcg
     1201 gtgcgttctt ccagcgtggc ttcggtcatc gtggtcgtcc ggggctcggg tctaaggggc
     1261 tcggttcggg tagagcggga aggaggagca gaggtcctcg acctgcgccc gaatcttggc
     1321 gagtgcggcc ggctcggaac gcttctggag ggcctcgtcg atccagcggc ccagcgtgcg
     1381 gaactcgggc tcgccgaggc cgcgcgtcgt gaccgcggcg gcgccgattc ggatgccgct
     1441 ggtcacggct ggaggacggg gatcgttcgg cacggcgttc ttgttcacgg tgatgccggc
     1501 cttgtggagc gcctcctcgg cctcggcgcc ggaaaatcca gcgttcctca ggtcgagcag
     1561 gatgagatgg ttgtccgtgc cgccggtcac gagctcgtag ccgtgcgtaa tgagctcctg
     1621 cgccagggtc ttcgcgttcg ccacgacctg cgcggcgtac tccttgaacg cgggcgtgga
     1681 ggcctcgcgg aagcagaccg ccttggccgc gatcacgtgc atgagcgggc cgccctgcat
     1741 tcccggaaat accgacttat caatcgcttt ggcgaactga ctcttgcaga ggatgaagcc
     1801 gcttcgcggt ccccgcaggg tcttgtgcgc ggtggaagtc accacgtccg cgtagggcac
     1861 ggggctcggg tgcaccccgc ccgcgacgag tccggcgacg tgcgccatgt cgaagacgaa
     1921 cgccgccccc acctcgtccg cgatcgcgcg gaacttcgcg tagtcgaaga accgtgggta
     1981 cgcgctcgcg ccggccacga tgacccgcgg cttgtgctcc cgcgccatcg cggccacctg
     2041 gtcgtaatcg aggagcccgg tgtcgggcga aaggccgtac tggaccgagc ggaagatgat
     2101 tccggagtgg ctcaccttgt gaccgtgcgt caggtgcccg ccgtgggcga gcgccattcc
     2161 gagcagcgtc gagcccggag gcatgagcgc gagataggtg ccgaggttgg cctgcgcccc
     2221 ggcgtgcggc tgaacgttcg catgctccgc accgaagagc gccttcgcgc gctcgatcgc
     2281 gagtgactcg gccttgtcca cgaactcgca gccgccgtag tagcgcttcc ccggatagcc
     2341 ctcggcgtac ttgttggtga gcggggagcc catggcctcg agcaccgcct cgctcacgaa
     2401 gttctccgag gcgatcagct cgagcgtggt ctgctggcgg tggacctcgt cgtcgatggc
     2461 gcgcgcgatc tcgggatcgg cggcatggag cgccacgtcg tgcgcgctca ccggcgcggc
     2521 ctcggagcgc gccatcagat cctgcgtccc gcccggatca ccgggccttc ttcgccgcgc
     2581 gctgaacctt ggtcttcgcg tcgaccggga agagccagcc gtggcggtcg cgcgcgcgtc
     2641 cctcgatgat gtcgaagaag gcgtcctgga gccgccgcgt catcgggccg accgtgccgc
     2701 tcccgacccc gatcctgtcg atggacgtga tcggagtgat ctcggccgcg gtaccggtga
     2761 agaagacctc gtccgcgatg tagagcgcct cgcgcgggat ctgctcctcg cgcacctcga
     2821 tgccgaggtc cttcgcgatc tggatgatgc tgtcgcgcgt gatgcccggc aggatcgcgt
     2881 tcgcgatggt cggcgtgtgg aggacgccgt tcctgacgag gaagatgttc tcgccgcttc
     2941 cctccgcgac gtagccgaag gagtcgagcg cgatcccttc ctgatagccg tcggtgatcg
     3001 cctcgagctt cacgagctgg ctgttcatgt agttcgcgcc ggtcttcgcg agcgccggga
     3061 acgtgtccgg agcggcgcgg cgccacgacg acaccttgac ggcgatgccg cgctcgagcg
     3121 cgtccttgcc caggtacttg ccccagttca gggtcgcgat cgcgacgtcc accggaacgc
     3181 ccgtgggatt cacgccgagg gtcttgaagc cgcgatagac cacggggcgg atgtagcagg
     3241 ccttgccgcc gttccgccgc accgtctcac gggtggcttc catcagctcg tcgtagaggt
     3301 atggaatgtc gagacgcgcg atcttgcagg agtcgaggag gcgctgcacg tgccggtcga
     3361 ggcggtagat cgcggtgccg tgcggcgtgt cgtacgcgcg aatcccctcg aaggcactgg
     3421 agccatagtg caccacgtgc gagagcacgt ggatcgtggc ttcctcgaac ggcacgaact
     3481 tgccgttcat ccagatcgtc cctctcactt cctctggcat tcgcttctcc ttccgccttg
     3541 aacgatgcgc cgctatcgga cgccttcccg ccgttcgacc gcctcacgga tgagcgggag
     3601 ccatcggagg agatggcttc gaatgcggtt gaaggtgtcg tcgtattcga ccgaggtgcc
     3661 gccgatcggg tcgtggattc cggtgcgcat ggcttccgct ccgtcggcac cctgttcggt
     3721 gatgaggtgg atccggtcgg ccgcgtccgg cgcgagctcc cgcgccctcg tgacgtgccc
     3781 cggctccatg cccaggatca gctcggattc gcggatcagc tcgggcgtca agctcgtgga
     3841 ttgatgcccg cgcagatcga tgtcccggga gccggtggtg ctgacggcga gcggggtggc
     3901 ggggacgcca ggaacggcgt tggtcccggc cgagcgcacg cgcacctggg ccgcgatctc
     3961 ggagggcagg agcgaccgca aagctccctc cgccatcggg ctccggcagg tgttcccggt
     4021 acagacgatg aggacttgga tcacgcgttt tggccctcgg gatgcgttcg taggctccgg
     4081 gtgagtctag ggttcggcct ctagaccgtc aaggcttcca tcccctagcc ggacggcgcc
     4141 ttggcgcagg atttcgaccc ccttcggccc gacccggacc agggtcgacg gccggccgtc
     4201 caggacccct ccgtcgacga tcaagtcggc caccccggat ggggcgtcgt ccgcccgcaa
     4261 ggccggcggc tccccgctcc ggttggcgga ggtcgagagg accggctccc cgactccttg
     4321 gacgacgccg cgcagcagat cgcttcccgg acagcggagg gcgatcgttc cgctctcgga
     4381 ccgaagcgac tcgggcgcct ccggccgcgc gtcgagaacc agagtcagcg cccccggcca
     4441 gtaccggcgc gcgagctcga gcgcatgggg cgatgcgtgc gcccaccgct cgagatcgcc
     4501 aggctcggcg atcaggccga tgaacccggc gcgggggtct ctcttgaggg cgcggatgcg
     4561 ctcgatcgcg atcgccgacg aggcgcgggc atggaagccg tacacgccct ccgccggcag
     4621 gagcgcgacc ttgtcctcga gaagcgcggc cgcggcgcgc gcgatcaggt ccgcgtcggg
     4681 gtgggcgccg atcgcgagcc ggaccgcact cacgacgcta gagcttctcg ccgcgggcga
     4741 ggcgcgcctt gaggtcctcg agcttcgtcc gcagcgcggg atccgagatc gcgaggatct
     4801 gcgccgcgag aacccccgcg ttcttcgccc ctgcggagcc gatcgctacc gtcgcgaccg
     4861 gaacgcccgc cggcatctgc accgtcgaga ggagcgcgtc cgctcccccg agcggcgagc
     4921 cgccgagcgg aacgccgatc acagggagcg acgtatgcgc ggccacggcg ccggccaggt
     4981 gattcgccat ccccgccgcc gcgacgaaca cctccacacc ccgcccgggc gcgccgcgca
     5041 cgatgtcccg caccttgtcc gggctccgat gcgccgacgc ctgctccatc tcgtagccga
     5101 tgccgaagga gtcgaggacc ttcgcggtct cctgcatcgt ggtccggtcg gattcgctcc
     5161 cgaagaggat cgcgaccctg gtcttcgtgc tcatggacgt gacgctccag aggtttgagg
     5221 ggactgcgcg atgtccgtgc gatactgcat gccgtcgaac cggatccgtc cgaccgcctg
     5281 gtaggcggcg aggcgggccc gctcgagcgt ttccgagcgc gcggtcacgc cgagaacgcg
     5341 gcccccgctc gtgatccact gcccgccgcg cgacgcgacg cccgcgtgat acacgcagat
     5401 gccgtgcggc caggggccgt tcaggccttc gatcacggcg cccgactccg gtgacgccgg
     5461 gtagccgcgc gccgcgagca cgacgcaaac cgccgccccc tcgtgcgcga cgaacgtgcc
     5521 gtgccgcgct tcaggcagac ggccacgcgc cgcttggtcg gcgagcggga gcaggtcctc
     5581 ggagacgatc gggagcaccg cctgcgcctc gggatcgccg aagcgcgcgt tcacttcgag
     5641 aatctgcggt ccctgggcgg tgagcatgag gccgagatag agaatccccc ggtacggcgt
     5701 gccctcggcg cgcagcgcgg ctaagagcgg gtcgacgagg gcatgagcga tcgccttggc
     5761 gtcctcgggc gcaggcacgc gcgcgggcga gcacgccccc atgcctcccg tgttcgggcc
     5821 ggtgtcgtta tctcgaagtc gcttgtgatc gcgcgccggc gggaagagca cccagcgctc
     5881 gccgtcggtg aggaggagga tcgaggcctc ctcgccttcg aggaactcct cgatgacgag
     5941 cgtgcgcccc gagtcgccga gcatgccgcg gcgcatccat ccgtcgatcg ccgccatcgc
     6001 ctcgtccttg ctcatggcga cgacgacgcc cttccccgcc gcgaggccat cggccttcaa
     6061 cacgatcggg aggggcccgg cgtcgaccat gcgctccgct tcctccgcgg atcgcgccac
     6121 ctggaaggac gcggaaggaa gccccgcctt ctgaaacagc tccttcgcga agaccttgct
     6181 cgtctcgagc cgcgcaccat cgcggcctgg gcccagcacg gggacgtggg cctcccgaag
     6241 cgcgtccgcg agccctcgct cgagcggaat ctcgggtccg acgacgacca agtcgatggc
     6301 gtgggagttg acccagccga cgagggaggg cacgtcgccc gcctcgatgg ccacgcgctc
     6361 ggcgagcggc tccatgccgg ggcttccggg ggcgacgaag atcttggggt ggtccgggga
     6421 ccggtggaga gcgtcgacga gcgcgtgctc gcgtcccccc tgcccgacga cgagaaccct
     6481 cggctcgctc atccctgctt ggcgagagcc gccttgagcg cggcctggag cttctccggg
     6541 tgcgcgcgac ctcccgagcg cttcatcgcc tggccgacga agaagccgaa ggtcttctcc
     6601 ttgcccgcgc ggaactcggc ggcgggaccg ggattcgcgg ccacgatctc ctccgcgagc
     6661 ttcaagagcg actcctcgtc gtcgatcgga aggaggccgt gcttcttcac gatctcttcg
     6721 ggggacgagg ggtcggtgag catgtgctcg aagaccgtct tcccgatctt ccccgagatc
     6781 gtcgcgtcgc cgatcatctt cacgagcgcg ccgaggcggt cggcctggat cgggaacgtc
     6841 tcgatggaga ggccgtcccg gttccggatc gcgttcacct cggtcatgat ccagttcgac
     6901 gcgagcttcg gccgcttcga caggcgcacg acctcctcgt agtacgtcgc gagcgagcgc
     6961 gtctcggtca gcacctcggc gtcgtactcg ggaatttcgt actcgcgcac gagccgctcc
     7021 cagagcgcgt gcggaagctc cggcagcgag gtccgcaccg attcgatcca cgcgggatcg
     7081 agatcgagtg gcaggagatc cggctccggg aagtagcggt agtcgtgcgc ctcttccttg
     7141 ctgcgcatcg attccgcgac gctccgctcg gagttccaga gaagggtttc ctgcaccacg
     7201 cgtccgccgg actcgatcaa gccgatctga cgctcgatct cgaacgcgat ggcgcggtcg
     7261 acgaagcgga acgagttcag gttcttgatc tcggtcttgg tgccgaacgt cgaggcgccc
     7321 ttcggacgca ccgacacgtt cgcgtcgcac cgaaggctcc cctcttccat gttgccgtcg
     7381 cacacgtcga ggtagacgag gatctgccgg aggcgcgcca tgtagtcggt cgcctcctcg
     7441 cccgagcgga tgtccggctc ggagacgatc tcgatcaggg gcacgccggc gcggttcaag
     7501 tcgacgcgcg tggtgtggac gccttccttc ccttccggat ggagcgactt ccccgcgtcc
     7561 tcctcgaggt ggatgcggac gagaccgatc cgtttccagc cctcccccga gcgaatctcc
     7621 aacgatccgc cttcacagag cgggcggtcg tactgggaga tctggtagcc cttcgggagg
     7681 tccggataga aatagttctt ccgcgcgaac acgctgcggg tcgagatctt ggaccccacc
     7741 gccagtccca ccatcacggc ataacgcacg gcgcgctcgt tgagcacggg aaggacgccc
     7801 ggaagtccga ggcagacggg acagacctgc gtgttgggcg gcgcgccgaa gcgcgtggag
     7861 cagccgcaga agatcttcga cgcagtcttg agctgcgcgt gcacctcgag cccgatcacg
     7921 ggctcgtagc gctcgcgcgc ggtggcggtg gcggccgggg agatcgcgct cacgccgcct
     7981 cctcggcgat cggagggatg cgccacgcct cgtcgctcgc tttttcccag gttcgcgcga
     8041 tacggaacag ggtcgtctcg tcgaagggct ttccgacgag ctggagtccg atcgggaggc
     8101 cgctcttcgt gaacccggcc gggaacgaca tcgcgggaat gccggcgagg gacgccggga
     8161 tcgagtagat gtcattcagg tacatggcga gcggatcgtc cgccttctcg ccgagcttga
     8221 acggcggcgt gggcgtcacc ggaaggagga tcgcgtccgc cttggcgaag gcctcggtga
     8281 agtcgcggcg gatgagggtg cgcactttct gggcccggag atagtaggcg tcgtagtacc
     8341 ccgccgacag cgcgtacgtg ccgaggagga tgcgccgggt gacctcgcgt ccgaagccga
     8401 ggccgcgcgt cgtctcgtac atcccttgaa ggtcgccgtt ccccgcgcgg agaccgtacc
     8461 gcacgccgtc gtagcgggcg aggttgctcg aggcttcggc cggagcgatg agatagtagg
     8521 tcgcgatggc gtacttcgcg tgcggcaggt ccacctcggc ctggcccgcg ccca
//
LOCUS       DASTKC010000012         4163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1194690, whole genome shotgun sequence.
ACCESSION   DASTKC010000012 DASTKC010000000
VERSION     DASTKC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194690"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFR25_00500"
     CDS             complement(<1..299)
                     /locus_tag="VFR25_00500"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET9939557.1"
                     /translation="MNARAKRGPARAGFDSISDTLRALRAGSMAVVVDDEDRENEGDF
                     VMAAEKVTAEDVNFLTRDARGLLCVSMPGVRLEELGLALMTPLNTARFSTPFTISV"
     gene            complement(296..919)
                     /locus_tag="VFR25_00505"
     CDS             complement(296..919)
                     /locus_tag="VFR25_00505"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET9939558.1"
                     /translation="MFSGIVERVGMVERVATEGGSRRLVVAAPFDAPLQAGESVAVNG
                     VCLTVERADARSFEAVAVRETLDKTTLGALESGSRVNLERALRVGQALGGHWLQGHVD
                     ATAAIRGVHRDAGQVRLQVEIPEACRRFVATKGSIAIDGTSLTVASLTDPIAEIAIVP
                     FTLEHTIASSYAPGTRVNLEVDLIARYLDRLLEARGSLLVGEPMRTR"
     gene            complement(912..2003)
                     /gene="ribD"
                     /locus_tag="VFR25_00510"
     CDS             complement(912..2003)
                     /gene="ribD"
                     /locus_tag="VFR25_00510"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9939559.1"
                     /translation="MRRAIRIAHRGVGTTHPNPRVGAVVLRGGRAVAEGFHARAGEAH
                     AEVRALDQAGDDARGATLVVTLEPCAHVGRTPPCTEAVLRAGIRRVVIGMRDPNPLVN
                     GRGIQELEQAGLDVVVGVLEEECRELNPAYLKHLATGLPWVTLKAMLSLDGRLATESG
                     ESRGLGSDLEIRAAHRMRAASDAVVAGIGTVLADDPRLTVRGVRGRCPARVVLDSKLR
                     IPAAARIWSTLADAPLVIATTSKDEARVRELEQRGASVWTFEPRADGRVPLRPLLERL
                     ALEGWISVLVEGGADVHTAFLREGLADQIAVGISPRIVGGRTAPILTGDLGRPRLADA
                     IRIEGLSVKRLGPDVWLTGRIAADGGSRV"
     regulatory      complement(2160..2287)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2319..>4163)
                     /locus_tag="VFR25_00515"
     CDS             complement(2319..>4163)
                     /locus_tag="VFR25_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9939560.1"
                     /translation="ITPPVAVGQADQVFLSMDAYRSLPLNACFYYNVEFRTRNVGSPG
                     WSAWIDPTGSLYFGAEHEWMRQTVALTGAGGVDSVQVRIGIKDWGPVFCEGSQTTSGT
                     DLMLDNFRIGTVGASGPSLAANEADLYNDTFRTTAFNGNDNFNTLSGDSLAVRVGAAN
                     GLKTASLFTSIANAPFTSVPLVKISGTENTYTADVPAGNYARGTVLRYYFSATDSLNQ
                     VATLPADAIGSSHYFSATILPGVYTTSGACPDDTARVLYVNSWGGPDLTSGVAQSLTA
                     IGARFDRFDVNAPQLAAGNSPGGGTIGGGTAVWPAAPLGVLGAYRAIIWDVGDRNAVT
                     LSAEDQSLLTSWLSLPGKNRGLFLAGDNLAYDLLVNGQGITNFLSCTLGANYVRDVWE
                     NAPQDTLIPIARGASGTRIANEPFPLDGGCPVINAFDATAVSSCVGANGRVWLRYPNN
                     LAAATERQAPNGPPNGDSLRVVVAGFSLANLTDPVRRNLLLYRTIVQEFETPSCYVAT
                     GIPEDATVASPRATLARLYAPSPNPFNPHTTIRFGLERRARVTLRIFAVDGSLVRTLV
                     DRPFEAGEHQVVWDGRDLRGREQASGAYFVSLEADGVRSPARKVILLR"
BASE COUNT          637 a         1464 c         1445 g          617 t
ORIGIN      
        1 acggagatcg tgaacggagt cgagaagcgc gcggtgttga gtggcgtcat gagggcgagg
       61 ccaagctcct cgaggcgcac cccgggcatc gacacgcaga ggagtccccg cgcgtcccgc
      121 gtgaggaagt tcacgtcctc ggccgtgacc ttctcggccg ccatgacgaa gtccccttcg
      181 ttctcccggt cctcgtcatc cacgaccacc gccatcgacc ccgcgcgaag cgcgcgcagc
      241 gtgtcggaga tcgaatcgaa tccggcccgg gcggggccgc gcttggcgcg tgcgttcatc
      301 gtgtcctcat aggctcgccc acgagaaggg agccgcgcgc ctcgagcagg cgatcgagat
      361 agcgggcgat gagatcgact tccaggttca cccgggttcc gggcgcgtag ctcgatgcga
      421 tcgtgtgctc gagcgtgaag ggcacgatcg cgatctccgc gatcggatcg gttaaggagg
      481 ccaccgtgag gctcgtgccg tcgatcgcga tggagccctt cgtcgcgacg aatcgacggc
      541 aggcctcggg gatttcgacc tggagccgga cctggccggc gtctctgtgg acgccgcgga
      601 tcgcagccgt cgcgtcgacg tgcccctgga gccagtgacc gccgagcgcc tgccccacgc
      661 gaagcgcgcg ctcgagattc acgcgcgagc ccgattcgag cgctccgagc gtggtcttgt
      721 cgagcgtctc gcgcacggcc acggcctcga acgagcgggc atccgcgcgc tccacggtga
      781 ggcacacacc gttcacggcc acgctctccc ccgcctggag cggcgcgtcg aagggcgccg
      841 cgacgacgag cctccggctt cccccttcgg tcgcgacgcg ctcgaccatc ccgacgcgct
      901 ccacgatgcc gctaaacacg cgatcctccg tcggccgcga tccgaccggt cagccacacg
      961 tcgggtccga gccgcttcac cgagagaccc tcgatccgga tggcgtcggc gagacgggga
     1021 cgtcccagat ccccggtcag gatcggcgcc gtccggcctc cgacgatgcg gggcgagatg
     1081 ccgaccgcga tctggtccgc caacccctcg cgaaggaacg cggtgtgcac atccgcgccg
     1141 ccctccacca ggaccgagat ccagccttcg agcgccaggc gctcgagcag ggggcgaagg
     1201 ggcacgcgcc cgtccgcccg cggctcgaac gtccagaccg atgcgccgcg ctgctcgagc
     1261 tcgcgaaccc gcgcctcgtc cttggacgtc gtcgcgatca cgagcggcgc atcggccagc
     1321 gtgctccaga tgcgggcggc cgccggaatc ctgagcttgg agtcgagcac gacgcgcgcc
     1381 ggacagcggc cgcgcacgcc ccgcaccgtg agtctcggat cgtcggccag cacggtcccg
     1441 attcccgcca ccaccgcgtc gctcgcggcg cgcatgcggt gggccgcgcg aatctcgagg
     1501 tcgcttccga ggcctcgcga ttcgccgctc tcggtggcga gccttccgtc gagcgagagc
     1561 atcgccttca aggtcaccca cgggagcccg gtcgcgagat gcttcaggta ggccggattc
     1621 agctcccggc actcctcctc gagcactccc accacgacgt cgagcccggc ctgctcgagc
     1681 tcctggatgc cgcggccgtt gacgagcggg ttgggatccc gcatgccgat gaccacccgg
     1741 cggatccccg cccggagcac ggcctcggtg caggggggcg tgcgtcccac gtgggcgcag
     1801 ggctcgaggg tcacgacgag cgtggcgccg cgcgcgtcgt cgcccgcctg gtcgagagcg
     1861 cgaacttcgg catgggcctc gccggcgcgc gcgtgaaagc cctcagctac ggcgcgaccg
     1921 ccccgaagca cgacggcgcc cacgcgcggg ttcggatgcg tcgtccccac gccccgatgc
     1981 gcgatccgga tggcgcggcg catggccgcg atctccgcgg cgtcgccgcg atcccgcgac
     2041 gcgccgcgac ccggcctatg ctctatggaa tcggtgtgta ggctcacaat gaaaaaaccc
     2101 cgcaggcaga cacccgcggg gcctgagcgg cgatggccgg acatgcccgt accgtgtgcg
     2161 acgtccttct cccatccaga ctttgactgt cggctccgga atcgcaccgg atctgccgtg
     2221 ggacgtaatc ccttggctcg cgggctctac cgccggtggg gactttcacc ccgccccgaa
     2281 ggatgtctgc taccccggag cctactccgg aaggcgcctc agcgcaagag gatgaccttg
     2341 cgggccgggc tccggacgcc atccgcttcg aggctcacga agtaggcgcc gctcgcctgc
     2401 tcccggccac ggaggtcgcg cccatcccaa acgacctggt gctccccggc ctcgaaggga
     2461 cgatcgacga gcgtccggac gagcgagccg tcgacggcga agatccgcag cgtgacccgc
     2521 gccctccgct cgaggccgaa gcggatggtc gtgtgcggat tgaacgggtt cggcgaggga
     2581 gcgtagaggc gcgcgagggt ggcccgcggc gacgcgacgg tcgcgtcctc cggaatcccg
     2641 gtggcgacgt agcagctcgg agtctcgaac tcctgcacga tggtccggta gaggagcagg
     2701 ttccgccgca ctggatcggt gagattggcc agcgagaacc cggcgacgac cacgcggaga
     2761 ctgtcgccgt tcggtggacc gttgggcgcc tggcgctccg tcgccgcggc gagattgttg
     2821 ggatagcgga gccacacgcg cccgttcgcg ccgacgcacg acgagaccgc cgtcgcgtcg
     2881 aaggcgttga tgacgggaca ccctccgtcg agcgggaacg gctcgttggc gatgcgggtg
     2941 ccggacgccc ccctcgcgat ggggatgagc gtgtcctgcg gcgcgttctc ccagacgtcg
     3001 cgcacgtaat tcgctccgag ggtgcaggag aggaaattcg tgatcccctg cccgttgacg
     3061 aggaggtcgt aggcgaggtt gtcccccgcg aggaagagcc ctcggttctt gcccggcaag
     3121 gagagccagc tcgtgaggag ggactgatcc tccgcgctca gcgtgacggc gttccggtcg
     3181 ccgacatccc agatgatcgc gcggtacgcg ccgagcacgc ccaggggcgc cgccggccag
     3241 accgccgtgc cgcccccgat cgttcccccg ccgggcgagt tccccgccgc gagctgcggc
     3301 gcgttgacgt cgaaccggtc gaagcgcgcg ccgatcgccg tgagcgactg agcgacgccg
     3361 gacgtgagat cggggccacc ccagctgttc acgtagagca cgcgcgcggt gtcgtcaggg
     3421 cacgcgccgc tcgtcgtgta cacgccggga aggatggtcg cgctgaagta gtggctcgac
     3481 ccgatggcgt cggcgggcag agtcgcgacc tgattcaacg agtcggtcgc cgagaagtag
     3541 tagcgaagca cggtgccgcg cgcgtagttc cccgccggca cgtccgccgt atacgtgttc
     3601 tccgtgcccg agatcttgac caggggcacc gacgtgaacg gtgcgttcgc gatgctcgtg
     3661 aacagggacg cggtcttgag gccgttcgcg gccccgaccc gcaccgcgag gctgtcgccg
     3721 ctcaacgtgt tgaagttgtc gttcccgttg aacgcggtcg tgcggaaggt gtcgttgtag
     3781 aggtcggcct cgttcgccgc gagcgacggg ccggacgcgc ccacggtgcc gatccggaag
     3841 ttgtcgagca tgagatcggt cccggacgtg gtctggcttc cctcacagaa aaccggtccc
     3901 caatccttga tgccgatccg gacctggacc gaatccacgc cgccggcccc ggtcagcgcg
     3961 acggtctgcc tcatccactc gtgctccgcc ccgaaataga gggatcccgt cggatcgatc
     4021 caagcggacc atcctgggct tcccacgttt cgcgtgcgga actcgacgtt gtagtagaag
     4081 caggcgttca ggggcaggct tcggtaggcg tccatggaga ggaagacctg atcggcctga
     4141 cccacggcga cgggcggggt gat
//
LOCUS       DASTKC010000013        13944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_248979, whole genome shotgun sequence.
ACCESSION   DASTKC010000013 DASTKC010000000
VERSION     DASTKC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248979"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFR25_00520"
     CDS             complement(<1..1026)
                     /locus_tag="VFR25_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET9939561.1"
                     /translation="MTPAPISHVRWAPGPLGLDLGTDPDAWIRSLSDLPRPWFVYESP
                     RGDCLVGSGEAARFSGHGPERFAEAARWLDAAATAARRDGAHTSPIAVGAFAFGDRAD
                     AVAWIPSTLLVREAEGGVRVQSWQGSVDMSPRAMAPSPWVDGDRDAWTAAVEAALRRI
                     EAGALEKVVLAQARVLNGGRPYDPLSSFLSLRERYPECHRFLIGMDDGTAFLGASPER
                     LVSLRGRAIDADAVAGTARVDGEDEAAAARHLLSNAKERNEHAIVAREIVAALRARGA
                     SADAEPEPSTLRLRHVLHLRSRIEGVAPDGLGVLDLVRAIHPSPAVLGTPRDAALELI
                     EAWEPRDR"
     gene            1078..1680
                     /locus_tag="VFR25_00525"
     CDS             1078..1680
                     /locus_tag="VFR25_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreH"
                     /protein_id="HET9939562.1"
                     /translation="MLDLAGSQLPLLLGFVLGLRHALDPDHLAAMGTIVSRERSLSRA
                     ALQGGLWGLGHTASLLGVGLAVILIGAPLPTSWSRWFELAVAAMLIVLGTLTLIRRRA
                     DPPARPGARRPVLVGLMHGLAGSGALTIFVLASIRSPWVALLTIAIFGVGSIGGMMLL
                     SGLLALPFAWTGAPKLGRTARTLAGIVSIAVGLYCGYVAA"
     gene            1771..2289
                     /locus_tag="VFR25_00530"
     CDS             1771..2289
                     /locus_tag="VFR25_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9939563.1"
                     /translation="MSKPSQRGLVTLLLIVVTLALGGWAFLDTFSNVGYAPTQPIPFS
                     HKLHAGTRKIPCLYCHSNAERSRHATIPSTNVCMNCHLVVRTDRPAIQTVTKLYEEGK
                     PIPWVRVHRVPDYVYFSHRWHVARGIACQTCHGQIQTEDVVRQAENLKMGWCISCHRQ
                     NKASQECNTCHM"
     gene            2277..5357
                     /locus_tag="VFR25_00535"
     CDS             2277..5357
                     /locus_tag="VFR25_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-cluster-containing hydrogenase"
                     /protein_id="HET9939564.1"
                     /translation="MSHVNGGPKPPKYWAKPMDLEADPGAENAMQEFAQPSNADAIAL
                     EAADPPSRREFLKLLGAGAAFAAAGCARKPVEKILPYVHAPEELVPGVPVYYASTCGE
                     CPAGCGVLVKTREGRPIKLEGNKAHPLNKGALCPRGQASLLNLYDPDRLQGPILYGRG
                     KGSKKPATWQAVDQRVMTALGEARGRGKVVLLSGTVTSPTTMALVKQFLASYPGAEHV
                     VYDAVSQDALLKAQELMYGARAVPRYRLDKADVLVTLGADPLGTMISPVEYARDFYGR
                     RRPEMGPMMRVIAIEPVSSLTGTNADERLRVRPDHLYPVAMAIANELLVAKPRGAAAP
                     GGATGAVGTFEAATVERRAGLPFGTIARLADELWAARGKSLVMAGSQAAPAEQAVPLQ
                     MAAGLLNSILGNEGVTVDGSHPSRQAAGSEEEVLQLVERMRAGEIGALLLYGVNPAYT
                     LPPAVGFAEALARVPFTLSFADRIDETAALVDVVAPDAHYLESWNDYEPRAGVRSLGQ
                     PAIAPLYDVRQFQDSLIVWGGGAPAGTSGSWHAYLRDRWRNDIYPTANAAAGFDLWWE
                     GVLREGVQVAQGADGNGSARGFRGEALSSLPALKPGNDAEGLQLALYTPITIYDGRFA
                     NNAWLQELPDPMSKCTWDNYVAIAPSRAKALGVWEYEMKADVVTVDVGHAKFDLPVHV
                     APGLHPDVVGIAIGYGRTAAGRVGNGVGQNAYRLAEATAGRIGMAGIPVRLTKTGRMA
                     PLAVVQMHNTTDHRPIVYDATYAEYQRDPRAGIEEPTHLPSMWTPHEYTGYKWGMAVD
                     LTTCIGCSACMVACMVENNVPVVGKSIVLRGREMHWIRIDRYYSGNADDPDFAYQPML
                     CQQCENAPCETVCPVLATVHSSEGLNIQVYNRCVGTRYCSNNCPYKVRRFNWFEYSNI
                     KEKSLRLALNPDVTVRSRGVMEKCTFCVQRIRDGKEHAKAMGTRVQDGDIVTACQQTC
                     PTDAIIFGDLNDPKSRIAQFAQNGRGYHVLAELNTRPRITYQTKIRNREEGASA"
     gene            5354..6760
                     /gene="nrfD"
                     /locus_tag="VFR25_00540"
     CDS             5354..6760
                     /gene="nrfD"
                     /locus_tag="VFR25_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9939565.1"
                     /translation="MSDLVAQGLAEPPLIDPRKTFGDVTAEVSAPLEKKPGMLWKVFF
                     GISLLLTLQFFAEIAYQMYKGIGIWGLNHPVGWAVDITSFVFWIGIGHAGTLISAILH
                     LFRQRWRTAINRSAEAMTIFAVMTAGLFPLIHLGRTWFAYWLIPYPNVRFLWVNFRSP
                     LVWDVFAVSTYLTISLCFWYTGLIPDFASIRDRSTGWRRGIYGVLSLGWRGSERHWQH
                     YERAYMMLAGLSTPLVLSVHSIVSFDFATSILPGWHTTIFPPYFVAGAIFSGFAMVVT
                     LLVILRKVFKMEDYVTIHHLENMNKIILVTGMMVGYAYATEFFVALYSGNPFERFTFL
                     NRAGGPFAWSYWIMVSCNVISPQIFWFKRVRRSLVVMFIVSILVNVGMWFERFTIIVT
                     SLHRDFLPSAWSYYKPTIFDYSVIVGSFGFFFMWFLLFCRFFPTISMAEVKSVLWSAK
                     KTAAGHPKEPMPEETVHA"
     gene            6753..7295
                     /locus_tag="VFR25_00545"
     CDS             6753..7295
                     /locus_tag="VFR25_00545"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET9939566.1"
                     /translation="MLNPFARKPAPSRGVLALYDDPDKLLRAATLAREHGFPDLDAYT
                     PYAVHGLSEALGVKKSWVPWVTLVMGLTGACLALFFEIWTSAIDWPINVGGKPMVSLP
                     AFIPITFEGGVLIGGTMTLAALLLACGLPNLGKPILDRDLTNDRFGLWVPEIGPEWNE
                     QRIINVLRGTHATDIRVVRV"
     gene            7288..7938
                     /locus_tag="VFR25_00550"
     CDS             7288..7938
                     /locus_tag="VFR25_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9939567.1"
                     /translation="MSDRRVLALSALLLLAAASTGCERRKQSTAMEYMPNMAYGPRVA
                     AQAEDPMRPGDPVMRNPVPGTVPVGYTPYRYAVGDSLQAGQDLVNPLPRTQAVLERGH
                     KVFMNTCVVCHGPEGDGQGYIVPIYPMPPSLHSLKVRNWPDGRIFHVITRGQNLMPSY
                     ATQILPEDRWAVIHYVRALERSHLPVDSTKTSSAPADTAKGSGGPAAKTAMKGAAR"
     gene            7935..9152
                     /locus_tag="VFR25_00555"
     CDS             7935..9152
                     /locus_tag="VFR25_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939568.1"
                     /translation="MSHGSAHAPNMEALRERVGPIRIPGGVQAMFVVLMALGVISFFV
                     EKSADPTRAYAGYLLGFWYFMSLGLAGGFFTAIHYATGATWSVVVRRVAESFMSYLPV
                     ALVLLLVVWLGIPNLYVWSSNAAGEAGHAVDITKGGYLSKPLFAVRSAIFLVVWCVFA
                     WYFIRNSTRQDETGDPKLTKASIRASSIFILVFAVTVTLASFDFLMSLEPTWFSTIFG
                     VYCFAGLWQAGLAALTIVVVLLRRQGALQGVVSRYHYWDLGKYMFAFSVFWMYIAFSQ
                     FMLIWYANLPEEIDYMIPRTFTGWGGVGIALMVLRFGFPFFALLHQRAKESEVWLLVN
                     AGVILIGHWIDLYWLILPAFSRQHVVLGWTEIGITLGFLGLFGFCLLRFFARYPVAPR
                     RDPMFEASVRFHG"
     gene            9149..10168
                     /locus_tag="VFR25_00560"
     CDS             9149..10168
                     /locus_tag="VFR25_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9939569.1"
                     /translation="MSHDPHGSHTPHGHDHEESLSKVLANTQFVITGIFILVIASLAY
                     VAGMHSAKGGHGGEGGGTSASPASKVDVQALIKDTPELIAKGKTLFAVNCASCHGNTG
                     HGDGPAAAALNPKPRDFTSGYWRYGGGEVRVVRTISEGSPGTGMAAFAGTIPIEDRFA
                     LAHYVRSLGPKVEEDKPEDLAWLGPTGGTGGESGGTATGGPAAPQPSQATMPIERAIA
                     LLAQPEPQTGVALTASSDRSEPGAGLYAERCASCHGGAGEGGVRVRMIGSAPYAYVVS
                     RSLGDAQGTWTGDAGAFERLILNGIPGYVMPANGDLSREALQNLHGYTLKLRALQQGA
                     RRTGS"
     gene            10174..10884
                     /gene="coxB"
                     /locus_tag="VFR25_00565"
     CDS             10174..10884
                     /gene="coxB"
                     /locus_tag="VFR25_00565"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9939570.1"
                     /translation="MTRFRRTHAIALAIGILALLGGVGTAWAWLVNPVTPVAHEVREL
                     FYKAVAVTMFFFALAEGILLVAVLRFRAKPGVPPATFHENVRLELVWTAIPALAMVVL
                     AGPAFSTLKYLETVPKSDLTIEIVGHQWFWEYRYPGQGIVFANEPLVIPANKIVAADV
                     TSIDVVHSWFMPEFGVKMDANPGRVNHTWFQVERPGTYRGQCAELCGVLHGDMLITVQ
                     VVSPEEFARWIEQKKKGA"
     gene            10889..12502
                     /gene="ctaD"
                     /locus_tag="VFR25_00570"
     CDS             10889..12502
                     /gene="ctaD"
                     /locus_tag="VFR25_00570"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET9939571.1"
                     /translation="MEAGTLTRPHATPGVHAETKPRSAILDWLLTTDHKKIGVMYIWL
                     AFSFFAIGGVFAMIMRAQLAAPGLHVVRPDIYNQLMSLHGTFMIFFWTIPVMAGIGNY
                     VVPIQIGARDMAFPRVNAFSFWLLIPAGLLMLSGLWLQGGAAAAGWTGYPPLTDRQFS
                     PGPGVDLWILGLHLAGMASILGAINFVVTILNLRAPGMSMMKMPMMSWNVMITQSIIL
                     VATPVLAGALTMLLTDRMFGTGFFHPSAGGDPVLWQHLFWFYSHPAVYIMVLPGMGFV
                     SQILPAFSSKKLFGYKGMVLATAGIGFIGFLVWGHHMFATGMDPTLRRFFSFASMVIA
                     VPTGIKIWSWLATVWGGSIRFRTPMLYGLGFIALFIIGGITGVWLALVPFDVQVHDTY
                     FVVAHLHYVLFGGSLMSIFAGIYYWFPKMSGRLYNEFWGKVQFWLTFIGMNLTFMPMH
                     MAGMLGMPRRIYTYQPQFKIWNEISSVGALILGVSMAIFMINMLHSLARGKRASADPW
                     DHDEFNKTLEWTVSSPPPAENFHTLPVIR"
     gene            12528..13415
                     /locus_tag="VFR25_00575"
     CDS             12528..13415
                     /locus_tag="VFR25_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9939572.1"
                     /translation="MDSRPGRLPALLSFAVVLTLVLVVWGGVVRLSGSGLAIPDWPLA
                     EGKLLPKPHPNVLIEYGHRVLAMLVSFTTLAIAIVVFRSSVYRSRLAGLTIALLITLV
                     LQVFMGARVVLEELPVDRVVAHLLLAFTFFGILVTMRHRAGDLERPRAGSGGRARGLA
                     RFAHIAAGFVFLQAGLGAWTSSSGAAMACPDFPTCHGMWLPPMQGLVGIHYAHRLGGY
                     VVFLTVLLLCLKSFSGPLPAGARWALRLSGILVAIQLLLGIGNVLLRVPLPVSAAHLG
                     TALVLFGALLTAAHELRRA"
     gene            13408..>13944
                     /gene="cyoE"
                     /locus_tag="VFR25_00580"
     CDS             13408..>13944
                     /gene="cyoE"
                     /locus_tag="VFR25_00580"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9939573.1"
                     /translation="MLDYIQLTKPRIVLLVIVTAVAGLVVEGSLLRDPARFALVLLAI
                     TMTAGSANAFNQYFERDLDATMDRTRLKRPLPRHRVPERGALAFAIAVGALSVILLAL
                     VANALSAALALATIVFYGFYYTLWLKPRTVHNIVIGGAAGAMGPVIAWAAASGTVALP
                     PVLMFLVIFLWTPPHFWAL"
BASE COUNT         2097 a         4814 c         4585 g         2448 t
ORIGIN      
        1 gcggtcgcgc ggctcccacg cttcgatcaa ttcgagcgcc gcgtcacgcg gcgttccgag
       61 gacggcgggg gacggatgga tggcccgcac cagatcgagc acgccgagcc catccggcgc
      121 aacgccctcg attcgcgagc gcaggtgcag cacgtggcgg agacggagcg tggacggctc
      181 gggctccgcg tcggcgctcg cgccccgggc gcggagtgcc gccacgatct cgcgagccac
      241 gatcgcgtgc tcgttccgtt ccttggcgtt actcaggaga tgtcgcgccg cggcagcctc
      301 gtcctctcca tcgacgcgtg ccgtcccggc gaccgcgtcg gcatcgatcg cgcgcccccg
      361 aagcgacacg agccgctcgg ggctcgcgcc caggaacgca gtcccgtcgt ccattccgat
      421 caggaatcgg tggcattcgg ggtagcgttc gcgcagcgag aggaaggacg agagcgggtc
      481 gtacgggcga cctccgttga gaacgcgcgc ctgcgcgagc acgaccttct cgagcgcgcc
      541 cgcttcgatt cgccggagcg ccgcctccac cgcggcggtc cacgcgtcgc gatcgccgtc
      601 gacccacggg gatggggcca tcgcgcgagg actcatgtcc acggatccct gccacgactg
      661 gacgcgaact ccgccctcgg cctcgcgcac gaggagcgtg gacggaatcc acgcgaccgc
      721 atcggctcga tcgccaaagg cgaacgcgcc gaccgcgatg gggctcgtgt gggcgccgtc
      781 gcgccgggca gcggtcgccg cggcgtcgag ccatcgcgcg gcctcggcga agcgctcggg
      841 accgtggccc gagaaacggg cggcttcgcc cgatcccacc agacaatccc cgcgtggaga
      901 ctcgtagacg aaccagggcc gcggaagatc gctcagggac cggatccagg catccggatc
      961 ggtgccgagg tcgagcccga gcgggccggg cgcccaacgg acgtgcgaga tcggagcggg
     1021 cgtcacagtc acttcgggag tatagatttt cgccccctgc tttcctatag tgccgcgatg
     1081 ctcgacctgg ccggctccca gcttccgctc ctcctgggct ttgtcctcgg gctcagacac
     1141 gcgctcgatc cggaccacct ggcggccatg ggcacgatcg tgagccgcga gcgcagcctc
     1201 tctcgcgccg cgctccaggg aggcctctgg ggcctggggc acacggcgtc gctcctcggc
     1261 gtcggactgg ccgtgatcct gattggcgcg cctcttccca cgtcctggtc gcgatggttc
     1321 gagctggctg tcgccgccat gctgatcgtg ctcggcacgc tcaccctgat ccgccgccgc
     1381 gccgatccgc ccgctcgtcc cggtgcccgg cgtccggtcc tggtcggact catgcacggt
     1441 ctcgcgggaa gcggggcgct cacgatcttc gtgctggcct cgatccgctc cccctgggtc
     1501 gcgctcctga cgatcgcgat cttcggcgtg gggtcgatcg gcgggatgat gctcctgagc
     1561 ggactcctcg cccttccgtt cgcctggacg ggggccccga agctgggccg gacggcccgg
     1621 accctggccg gcatcgtcag cattgctgtg ggtctctatt gcggttacgt tgccgcttga
     1681 cgggctcgaa gttggtgatt agattcacaa agttttctcc caggatggaa ggctcgaact
     1741 ccccccggag ataggatcta gctcagagcg gtgtcgaagc cgtcgcagcg agggctcgta
     1801 accctcctct tgatcgtcgt cacgctcgcg ctcggcggat gggcgttcct cgacacgttc
     1861 agcaacgtgg gatacgcccc tacccagccg attcccttca gccacaagct ccatgccggg
     1921 acgcgcaaga ttccctgcct ctattgccac tcgaacgcgg agcggtcgcg ccacgcgacc
     1981 atcccgtcga cgaacgtctg catgaactgc catctcgtcg tgcgcaccga caggcccgcg
     2041 atccagaccg tcaccaagct ctatgaagag gggaagccga tcccgtgggt ccgcgtccac
     2101 cgcgttcccg actacgtcta cttcagccac cgctggcacg tcgcgcgggg cattgcctgc
     2161 cagacctgcc atggacagat ccagaccgag gacgtcgtgc gccaggcgga aaacttgaag
     2221 atgggctggt gcatcagctg ccaccgccag aacaaggcct cgcaggagtg caatacatgt
     2281 cacatgtaaa cggcgggccg aagcctccca agtactgggc gaagcccatg gatctcgagg
     2341 ccgacccggg cgccgagaat gcgatgcagg agttcgcgca gccgtcgaac gcggacgcga
     2401 tcgcgctcga ggcggcggat ccgccttcgc gccgcgaatt cttgaagctc ctcggcgccg
     2461 gcgcggcgtt tgccgcggcc gggtgcgcgc ggaagccggt cgagaagatc ctcccgtacg
     2521 tgcacgctcc cgaggagctg gtcccgggcg tgcccgtcta ctacgcctcg acctgcggcg
     2581 agtgcccggc ggggtgcggc gtgctcgtga agacgcgcga agggcgcccg atcaagctgg
     2641 aggggaacaa ggcccacccg ctcaacaagg gcgcgctctg cccgcgcggc caggcctcgc
     2701 tcctcaatct ctacgatccc gaccggctgc aggggccgat cctctacgga cgcggcaagg
     2761 gctcgaagaa gcccgcgacc tggcaggcgg tcgatcagcg cgtgatgacg gccctcggcg
     2821 aggcgcgcgg acgcggcaag gtggtgctcc tcagcggcac cgtgacgagc ccgacgacga
     2881 tggcgctcgt gaagcagttc ctcgcgtcct atcccggcgc ggagcacgtc gtctacgacg
     2941 ccgtgtcgca ggacgcgctc ctcaaggcgc aggagctcat gtacggagcc cgcgccgtgc
     3001 cgcgctaccg cctcgacaag gccgacgtcc tcgtcacgct cggagcggat ccgctcggca
     3061 cgatgatctc gccggtcgag tacgcgcgcg acttctacgg acgccgccgc cccgagatgg
     3121 gcccgatgat gcgcgtgatc gcgatcgagc ccgtctcctc gctcaccggc accaacgcgg
     3181 acgagcggct ccgggtgcga ccggatcatc tctacccggt ggcgatggcc atcgcgaacg
     3241 agctcctcgt tgccaagcct cgcggcgcgg cagctccggg cggcgcgacc ggcgcggtcg
     3301 gcacgttcga ggccgcgacc gtggagcgtc gcgccgggct tccgttcggc accatcgcgc
     3361 ggctcgcgga cgagctctgg gccgctcgcg ggaagagcct cgtcatggcc ggctcgcagg
     3421 cggcgcctgc ggagcaggcg gtgccgctcc agatggcggc cggcctcctg aacagcatcc
     3481 tcggaaacga gggcgtgacc gtggacggat cccatccgtc ccgccaggcc gccggctccg
     3541 aggaagaggt gctccagctc gtcgagcgga tgcgcgccgg agagatcggt gcgctgctcc
     3601 tctatggcgt gaatcccgcg tacacgctgc ctccggcggt cggatttgcc gaggcgctcg
     3661 cgcgggttcc gttcacgctg agcttcgccg accgcatcga cgagacggcg gcgctcgtcg
     3721 acgtcgtcgc gccggacgcc cactacctcg aatcgtggaa cgactacgag ccgcgcgccg
     3781 gcgtgcggtc gctggggcag ccggctatcg cgcccctcta cgacgtgcgc cagttccagg
     3841 actcgctcat cgtgtggggc ggcggcgcgc cggcgggaac gtcggggagc tggcacgcct
     3901 acctgcgcga tcggtggcgg aacgacatct acccgacggc gaacgcagcg gccgggttcg
     3961 atctctggtg ggaaggcgtc cttcgcgaag gggtccaggt cgcccagggc gcggatggaa
     4021 acgggagcgc gcggggattc cgcggagagg cgctgtcgtc cctgccggcg ctcaagccgg
     4081 gcaacgacgc cgagggcctc cagctcgcgc tctacacgcc catcacgatc tacgacggcc
     4141 gcttcgcgaa caacgcctgg ctccaggaat tgccggatcc catgtcgaag tgcacgtggg
     4201 acaactacgt cgccatcgcg ccgtcgcgcg cgaaagcgct cggcgtctgg gaatacgaga
     4261 tgaaggcgga cgtcgtcacc gtcgacgtgg ggcacgccaa gttcgatctc ccggtgcacg
     4321 tcgctcccgg gctccatccc gacgtggtcg ggatcgcgat cggatacggc cgtacggccg
     4381 cggggcgtgt ggggaacggc gtcggccaga acgcgtaccg gctcgccgag gcgaccgccg
     4441 gacggatcgg catggccggc atcccggtcc gcctcaccaa gaccgggcgg atggcgccgc
     4501 tcgcggtggt gcagatgcac aacacgacgg accaccggcc gatcgtctac gacgcgacct
     4561 acgccgagta ccagcgcgat ccgcgcgcgg gcatcgagga gccgacgcac ctcccgtcga
     4621 tgtggacgcc gcacgaatac accggctaca agtggggcat ggcggtcgat ctgaccacct
     4681 gcatcggctg ctccgcctgc atggtcgcgt gcatggtcga gaacaacgtc ccggtggtgg
     4741 ggaagtcgat cgtgctccgc ggccgcgaga tgcactggat ccggatcgac cggtactact
     4801 cggggaacgc ggacgatccc gacttcgcct accagccgat gctctgccag cagtgcgaga
     4861 acgcaccctg cgagacggtc tgcccggtgc tcgcgaccgt gcactcgagc gaagggctca
     4921 acatccaggt ctacaaccgc tgcgtcggca cccggtactg ctcgaacaac tgcccctaca
     4981 aggtgcgccg gttcaactgg ttcgaataca gcaacatcaa ggagaagtcg ctccggctgg
     5041 cgctcaatcc ggacgtgacc gtccggagcc gaggcgtcat ggagaagtgc accttctgcg
     5101 tccagaggat ccgggacggc aaggagcacg cgaaggcgat ggggacgcgg gtccaggacg
     5161 gcgacatcgt caccgcgtgc cagcagacct gcccgaccga cgcgatcatc ttcggcgacc
     5221 tgaacgatcc gaagagccgg atcgcgcagt tcgcgcagaa cggacgcggc taccacgtgc
     5281 tcgcggagct gaacacgcgc ccgcggatca cataccagac caagatccgg aatcgtgagg
     5341 agggagcgag cgcgtgagcg atctcgtcgc ccagggtctc gccgagccgc cgctcatcga
     5401 tccgaggaag accttcgggg acgtcacggc cgaggtctcg gctccgctcg aaaagaagcc
     5461 ggggatgctc tggaaggtgt tctttggaat cagcctcctc ctgaccctcc agttcttcgc
     5521 cgagatcgcc taccagatgt acaaggggat cgggatctgg gggttgaacc atccggtggg
     5581 atgggccgtc gacatcacga gcttcgtgtt ctggatcggc attggccacg cgggcaccct
     5641 gatctcggcc attctccatc tcttccggca gcgctggcgc accgccatca accgctcggc
     5701 cgaagccatg acgatcttcg cggtcatgac ggcgggactc ttcccgctca tccatctcgg
     5761 ccgcacgtgg ttcgcgtact ggctcatccc gtatcccaat gtgcggttcc tgtgggtcaa
     5821 cttccgctcg ccgctcgtgt gggacgtatt cgcggtctcg acctacctga cgatctcgct
     5881 ctgcttctgg tacacggggc tgattcccga cttcgcatcg atccgggatc gctcgacggg
     5941 ctggcggcgc gggatctacg gcgtgctctc gctcgggtgg cgcggctccg agcggcactg
     6001 gcagcactac gagcgcgcct acatgatgct ggcgggcctc tcgacgccgc tcgtgctctc
     6061 ggtgcacagc atcgtgtcct tcgacttcgc gacctcgatc ctcccgggat ggcacacgac
     6121 gatcttcccg ccctacttcg tggcgggggc catcttctcc gggttcgcca tggtggtgac
     6181 gctgctcgtg atcctccgga aggtcttcaa gatggaggac tacgtcacga tccaccacct
     6241 cgagaacatg aacaagatca tcctggtcac gggcatgatg gtgggctacg cgtacgccac
     6301 cgagttcttc gtggccctgt acagcgggaa tccgttcgag cggttcacgt tcctgaaccg
     6361 cgcgggcggc cccttcgcct ggtcctactg gatcatggtg agctgcaacg tgatctcgcc
     6421 gcagatcttc tggttcaagc gcgtcaggcg gagcctcgtg gtcatgttca tcgtatcgat
     6481 cctggtcaac gtggggatgt ggttcgagcg gttcacgatc atcgtgacct cgctccaccg
     6541 cgacttcctc ccgtcggcct ggtcctacta caagcccacg atcttcgatt actcggtcat
     6601 cgtcggctcg ttcggattct tcttcatgtg gttcctgctc ttctgccgct tcttcccgac
     6661 catctcgatg gccgaggtga agagcgtcct ctggagcgcg aagaagaccg cggcgggcca
     6721 cccgaaggag ccgatgccgg aggagacggt ccatgcttaa tcccttcgcc cggaagccgg
     6781 cgccgagccg gggcgtgctc gccctgtacg acgacccgga caagctcctc cgggcggcga
     6841 cgctcgcgcg ggagcacggg ttcccggacc tggacgcgta cacgccgtac gccgtgcacg
     6901 gcttgagcga ggcgctcggg gtcaagaagt cgtgggttcc gtgggtcacg ctcgtgatgg
     6961 ggctcacggg cgcatgcctc gccctcttct tcgagatctg gacctccgcg atcgactggc
     7021 ccatcaacgt gggcggaaag ccgatggtct ccctcccggc cttcatcccg atcacgttcg
     7081 aaggcggggt gctgatcggc gggaccatga cgctcgccgc gctcctcctc gcctgcgggc
     7141 tgccgaatct gggaaagccg atcctcgacc gggatctcac gaacgaccgc ttcggtctct
     7201 gggtgcccga gatcggcccg gagtggaacg agcagcgcat catcaacgtg ctccgcggaa
     7261 cgcacgcgac ggacatccgg gtggtccgtg tctgatcgcc gcgtcctcgc gctttcggcg
     7321 cttctcctcc tggcggccgc ctcaaccgga tgcgagcgcc ggaagcaatc gaccgcgatg
     7381 gagtacatgc ccaacatggc ctacggtcca cgcgtcgcgg cgcaggctga ggatccgatg
     7441 cgtcccggcg atcccgtgat gcggaacccg gtgccgggca ccgttccggt ggggtacacg
     7501 ccgtaccggt acgctgtcgg ggacagcctc caggccgggc aggatctcgt gaatccgctc
     7561 ccgcgcacgc aggccgtgct cgagcgcggc cacaaggtct tcatgaacac ctgcgtcgtc
     7621 tgtcacggac cggagggtga cggccaggga tacatcgtgc cgatctaccc gatgccgccg
     7681 agcctccatt cgctgaaagt gcggaactgg ccggacggcc ggatcttcca cgtgatcacg
     7741 aggggtcaga acctgatgcc gagctacgcg acgcagatcc ttccggagga ccgctgggcc
     7801 gtgatccatt acgtgcgggc cctcgagcgg tcgcacctgc cggtggactc gacgaagacg
     7861 tcgtccgcgc ccgccgacac ggcgaagggg tcgggcggcc cggcggccaa gaccgcgatg
     7921 aagggagcgg cgcgatgagc cacggctcgg cccacgcccc gaacatggaa gcccttcgcg
     7981 agcgcgtggg cccgatccgg atcccgggcg gggtccaggc tatgttcgtc gtcctgatgg
     8041 cgctcggcgt gatcagcttc ttcgtggaga agtcggccga tcccacacgc gcgtacgcgg
     8101 gctacctgct cggcttctgg tacttcatga gcctcgggct cgcggggggc ttcttcaccg
     8161 ccatccatta cgcgacgggt gcgacgtgga gtgtcgtggt gcgccgggtc gccgagtcct
     8221 tcatgtcgta cctgccggtg gcgctcgtgc tcctgctcgt ggtgtggctc ggcattccca
     8281 acctctacgt ctggtcgtcg aacgcggccg gcgaggcggg gcacgcggtc gacatcacga
     8341 agggggggta tctctcgaaa cccctcttcg cggtgcggag cgcgatcttc ctcgtggtct
     8401 ggtgcgtctt cgcctggtac ttcatccgga actcgacgcg ccaggacgag acaggagatc
     8461 ctaagctgac caaggccagc atccgggcct cgtcgatctt catcctcgtc ttcgccgtta
     8521 ccgtgacgct cgcctcgttc gacttcctca tgtcgctcga gccgacctgg ttcagcacga
     8581 tcttcggcgt ctactgcttc gccgggctct ggcaggcggg tctcgccgcc ttgacgatcg
     8641 tggtcgtgct gctccggcgc cagggcgcgc tccagggcgt cgtcagccgc tatcactact
     8701 gggatctggg caagtacatg ttcgcgttct ccgtcttctg gatgtacatc gccttctccc
     8761 agttcatgct catctggtac gccaacctgc ccgaggagat cgactacatg atcccgcgga
     8821 cattcacggg ctggggcggt gtcggaatcg cgctcatggt gctccggttc ggattcccgt
     8881 tcttcgcgct cctgcaccag cgggcgaagg agagcgaggt gtggctcctc gtcaacgcgg
     8941 gcgtcatcct gattggccac tggatcgatc tctactggct catcctcccg gcgttctccc
     9001 ggcagcatgt cgttcttgga tggaccgaga tcgggatcac gctcgggttc ctggggctct
     9061 tcggcttctg tcttctccgg ttcttcgccc gctatccggt ggcgccgcga cgcgacccga
     9121 tgttcgaggc gagtgtgagg ttccacggat gagccacgac ccgcacggct cgcacacacc
     9181 gcacggacac gatcacgagg aatcgctctc caaggtcctc gcgaacacgc agttcgtcat
     9241 cacggggatc ttcatcctcg tcatcgccag cctcgcctac gtcgccggga tgcactcggc
     9301 gaagggagga cacggcggcg agggcggcgg cacgtcggcc agccccgcgt cgaaggtgga
     9361 cgtccaggcg ctcatcaagg acacgcccga gctcatcgcg aaggggaaga cgctgttcgc
     9421 cgtgaactgc gcctcctgcc acggcaatac cggccacggg gacggtcccg ccgcggcggc
     9481 cctgaatccg aagccgcgcg acttcacgtc gggctactgg cgctacggcg gcggcgaggt
     9541 tcgcgtggtc cggacgatca gtgaaggctc gccgggaacg gggatggccg cattcgcggg
     9601 aacgattccg atcgaggacc gctttgcgct cgcgcactac gtccgctcgc tcggcccgaa
     9661 ggtggaagag gacaagcccg aggatctggc gtggctcggt ccgacgggcg gcaccggcgg
     9721 cgagtcgggc ggcacggcga ccggcggccc cgccgcgccg cagccctcgc aggcgaccat
     9781 gccgatcgag cgcgcgatcg cgctcctcgc acagcccgag ccccagacag gcgtcgcgct
     9841 gacggcatcg tccgatcgga gcgagcccgg cgccggcctc tacgccgagc gctgcgcctc
     9901 gtgccacggc ggcgcaggcg aaggcggcgt tcgggtccgt atgatcggct ccgcgccgta
     9961 tgcctacgtc gtgtcgcgca gcctgggtga tgcacagggg acttggaccg gcgacgcggg
    10021 agcgttcgag cggctcatcc tgaacggaat tccgggctac gtcatgccgg ccaatggcga
    10081 tctctcccgg gaggcgctcc agaacctcca cgggtataca ctcaagcttc gcgcgctcca
    10141 gcagggcgcg cgccgcaccg ggagctgacc acgatgaccc gtttccgccg cacccacgcg
    10201 atcgcgctcg ccattggaat cctcgcgctc ctcggaggag tgggcacggc gtgggcctgg
    10261 ctcgtcaatc cggtgacccc ggtcgcgcac gaggtccggg agctcttcta caaggcggtc
    10321 gcggtcacga tgttcttctt cgcgctggcc gaggggatcc tcctcgtcgc cgtgctgcga
    10381 ttccgcgcca agccgggcgt gccgccggcc acgttccacg agaacgtcag gctcgagctc
    10441 gtatggacgg cgatcccggc gctcgccatg gtcgtcctgg ccggtccggc gttcagcacg
    10501 ctcaagtatc tcgagacggt gcccaagtcc gacctcacca tcgagatcgt cggacaccag
    10561 tggttctggg agtaccggta tccggggcag ggcatcgtgt tcgcgaacga gccgctcgtg
    10621 atcccggcca acaagatcgt cgccgcggac gtgacttcga tcgacgtcgt gcacagctgg
    10681 ttcatgccgg agttcggcgt gaagatggac gccaatccgg gccgcgtgaa ccacacctgg
    10741 ttccaggtgg agaggcccgg cacctaccgt ggacagtgcg cggagctgtg cggcgtgctc
    10801 cacggggaca tgcttatcac cgtgcaggtc gtctcgcccg aggaattcgc ccgctggatc
    10861 gagcagaaga agaagggagc ctaggaccat ggaagccgga acgctgaccc ggccgcacgc
    10921 cacgccgggc gtccacgccg agaccaagcc gaggagcgcc atcctcgact ggctcttgac
    10981 gaccgatcac aagaagatcg gcgtgatgta catctggctg gccttctcct tcttcgcgat
    11041 cgggggcgtc ttcgccatga tcatgcgggc gcagctcgcg gcccccgggc tccacgtcgt
    11101 ccgtcccgac atctacaacc agctcatgtc ccttcacggg acgttcatga tcttcttctg
    11161 gacgatcccg gtcatggccg gcatcggaaa ctatgtcgtg ccgatccaga tcggcgcccg
    11221 cgacatggcc ttcccgcgcg tcaacgcgtt ctcgttctgg ctcttgatcc cggccgggct
    11281 cctcatgctc tccggtctat ggctccaggg cggcgccgcc gcggcaggct ggaccggcta
    11341 tccgccgctc accgaccggc agttctcgcc ggggccgggc gtggatctct ggatcctggg
    11401 cctccatctc gcaggcatgg cctccatcct gggcgcgatc aacttcgtgg tcacgatcct
    11461 caacctgcgc gctcccggca tgtcgatgat gaagatgccg atgatgtcgt ggaacgtcat
    11521 gatcacgcag tcgatcatcc tggtcgcgac cccggtgctc gccggcgcgc tcacgatgct
    11581 cctcacggat cgaatgttcg gcaccgggtt cttccatccg tccgcgggcg gggacccggt
    11641 cttgtggcag cacctcttct ggttctactc ccatccggcg gtctacatca tggtgctccc
    11701 gggcatggga ttcgtctccc agatcctccc tgcgttctcg tcgaagaagc tcttcggcta
    11761 caagggcatg gtcctcgcga cagcgggcat cggcttcatc gggttcctcg tctggggcca
    11821 ccacatgttc gcgaccggga tggatccgac gctccgccgg ttcttcagct tcgcgtcgat
    11881 ggtcatcgcg gtgccgacgg gcatcaagat ctggtcctgg ctcgccaccg tgtggggtgg
    11941 atcgatccgg ttccgcacgc cgatgctcta cgggctcgga ttcatcgcgc tcttcatcat
    12001 cgggggcatc accggcgtgt ggctcgcgct cgtgccgttc gacgtgcagg tccacgacac
    12061 ctacttcgtc gtcgcgcacc tccactacgt gctcttcggc ggctcgctca tgtcgatctt
    12121 cgcggggatc tactactggt tcccgaagat gtcgggacgc ctctacaacg agttctgggg
    12181 caaggtgcag ttctggctca ccttcatcgg aatgaacctc acgttcatgc cgatgcacat
    12241 ggcgggcatg ctggggatgc cgcgccggat ctacacgtac cagccgcagt tcaagatctg
    12301 gaacgagatc tcctccgtgg gcgcgctgat cctcggcgtc tccatggcca tcttcatgat
    12361 caacatgctc catagcctcg cgcgcgggaa gcgcgcttcg gcggatccct gggaccacga
    12421 cgagttcaac aagacgctcg agtggacggt gtcctcgccg ccgccggcgg agaatttcca
    12481 cacgctgccg gtcatccggt agcggaccgg ccgggctccc ggacaggatg gattcgcggc
    12541 cgggccgtct gcccgccctc ctcagcttcg cggtcgtcct cacgctggtc ctggtggtct
    12601 ggggcggcgt cgtgcgcctg agcggctcgg ggctcgcgat ccccgactgg ccgctcgcgg
    12661 aagggaagct tcttccgaag ccgcacccca acgtcctgat cgagtacgga catcgcgtgc
    12721 tcgcgatgct ggtcagcttc accaccctcg cgatcgcgat cgtggtcttc cggtcctccg
    12781 tctatcgctc gcgcctcgcc ggcctcacga tcgcccttct gatcacgctg gtcttacagg
    12841 tattcatggg agcccgggtg gttctcgagg agctgccggt ggaccgcgtc gtcgcgcatc
    12901 tcctcctcgc attcacattc tttgggattc tggtcacgat gcgccacagg gcgggggatc
    12961 tggagcggcc tcgagcaggc tccgggggcc gagcgcgagg gcttgcccgg ttcgcccaca
    13021 tcgcggcggg attcgtcttc ctccaggccg gcctgggcgc ctggacatcg tcctcgggcg
    13081 cggccatggc ctgtccggac tttccgacct gccatggcat gtggctccca ccgatgcagg
    13141 ggttggtggg catccactac gcccaccggc tcggcggtta cgtcgtgttc ctgaccgtgc
    13201 tcctgctctg cctcaaatcg ttttcggggc cgctcccggc gggagcccgg tgggctctcc
    13261 ggctttcggg tatactcgtc gccattcagc tcctactcgg tatcggcaac gtgctcctac
    13321 gggtgccgct gcccgtgagc gccgcccatc tcgggaccgc gctcgtcctc ttcggggcgc
    13381 ttctgaccgc ggcccacgag ctacgccgtg cttgattaca tccagctcac gaaaccccgc
    13441 atcgtcctgc tcgtcatcgt gacggcggtg gccggactcg tcgtcgaggg ctcgctcctt
    13501 cgcgatccgg ccaggtttgc gctcgtcctc ctcgcgatca cgatgacggc gggatccgcg
    13561 aatgcgttca accagtactt cgagcgcgac ctggacgcca cgatggatcg gacgcgcctg
    13621 aagcgccccc tgccgcgcca tcgggtgccg gagcggggcg cgctcgcatt cgcgatcgcg
    13681 gtgggcgctc tgtcggtcat cctgctggcc ctcgtggcga acgcgctcag cgccgcgctc
    13741 gcgctcgcca cgatcgtctt ctacggcttc tactacaccc tctggctcaa gccgcggacg
    13801 gtgcacaaca tcgtgatcgg cggcgcggcg ggagccatgg ggccggtgat cgcgtgggcg
    13861 gcggcgagcg gcacggtcgc gctcccgccc gtcctcatgt tcctcgtgat cttcctgtgg
    13921 acgccgcccc atttctgggc gctc
//
LOCUS       DASTKC010000014         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1668829, whole genome shotgun sequence.
ACCESSION   DASTKC010000014 DASTKC010000000
VERSION     DASTKC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668829"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..2123
                     /locus_tag="VFR25_00585"
     CDS             402..2123
                     /locus_tag="VFR25_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET9939574.1"
                     /translation="MPSVFISYRHDDSFSSAKLLVEHLKRWLPDSDLFLDESEIEGGE
                     DWRAILATQIDRAQVVLVVIGTRWMAIEQPGGHRRLDDPDDVVRWEIARAVAARKRII
                     PVLVDGGRLPAEADLPSALQSLARLQYCEIGRRTFEGDIQSLARTLSAESAAADPARF
                     IFLVKRSLVVVPVVSAVLCAVAWSNLFAKVEIWFANRITWLGDGLFDIPLSDQLRIVS
                     LSLEPRDGSGSDPSREAVRLDPSRRADFAALLDELVRQGARVVVFDVTLTQDTSFDDR
                     LAESVKGAVARGVQVGFGFKAFDAATNQPRIASAIARAGAALGVVCVGDRGGGGRNVA
                     FATLALIRGERAYPGLPLIAAYGSVQLPGLTAASSEVQVPGARAPIPISLTETFDEYA
                     PLCPARAAGTSMVRFIPHLSSREALRQPTVRHSLMDVVRSAGRGDAVFRAKTVLVGAE
                     QARDILDTPLDLAGSRYGFEFQADAVNALLTGRIVTPVKRTGQVISVVGVAAFAALLY
                     LSGPGRSRRRLAWVPWCVAVLSFVLAVLAYRFLDSLWSPVFPAAACLVTWAVLGFLDR
                     RWTHDER"
     gene            2302..>2618
                     /locus_tag="VFR25_00590"
     CDS             2302..>2618
                     /locus_tag="VFR25_00590"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9939575.1"
                     /translation="MTATSPGVRIERSGQPMPYTPNMVVREGDRIRTGSDTYAAIDFD
                     DGNTVYLNHDTWVTIGSIRVLLGEIFNAVVRVGAGSEVYTNDLTAAAEGTKFLLRIDP
                     VSTT"
BASE COUNT          390 a          903 c          864 g          461 t
ORIGIN      
        1 tcgcctctct cttgcctcgg caacttgagg tgtggcgtcc cctctttagg gcgctcctgg
       61 gggcgcacgt ccaagtctgg cagattccgt cagggggtcg gcgatcaccc gcgggggcga
      121 ccgcccccgc agcgtgctac gctctccgcg aggcactggt gatcgggcga acttcgccag
      181 ggctgaggaa gtattatccg taccgaatga aaggaccgaa ccgaacgaat gtacgctccg
      241 cgatcggtcg gaagtagtca agatggcgca tcgactcgtg ctccactcgg ggcggcttgc
      301 ctcgcaaggc cggcagcgac tatgctcgac tcggcgaccg cggaccgcgc gcgtccgaga
      361 ccaaccatgc ggcacgctag ctgaggcact ccccgagcgc catgccgagc gtgttcatca
      421 gctatcggca cgacgatagc ttctcgagcg ccaagctcct ggtcgagcac ctcaaacgct
      481 ggctcccgga ctccgacctg ttccttgacg aatcggaaat cgaggggggc gaggactggc
      541 gcgcaatcct tgccacgcag atcgaccgcg cgcaggtcgt cctcgtggtg atcggcacgc
      601 ggtggatggc gatcgagcag ccgggcggac accgccgcct cgacgatccc gacgacgtgg
      661 tgcgatggga gatcgcgcgc gccgtcgccg cgcgcaagcg catcatcccg gtcctggtcg
      721 acggcggacg gctgcctgcg gaagccgacc tgccgtcggc gctccaatcg ctcgcgcgtc
      781 tgcagtactg cgagatcggc aggcggacgt tcgaaggcga catccagagc ctcgcgcgga
      841 cgctcagcgc cgaaagcgcg gcggccgatc cggctcgttt catcttcctc gtgaagcgct
      901 cgctcgtcgt cgtgccggtc gtttccgctg tactgtgcgc cgtagcgtgg tccaatctct
      961 tcgccaaggt cgagatctgg ttcgcgaacc gcatcacgtg gctcggcgac gggctcttcg
     1021 atatcccgct cagcgatcaa ctccggatcg tttcgctgtc gctcgaaccg cgggacggat
     1081 cgggctcaga tccgtcgagg gaagcggtgc gactagatcc gtcgcgccgc gcggatttcg
     1141 ccgcgcttct ggacgagctg gtccgccagg gcgctcgcgt cgtggtcttc gacgtgacct
     1201 tgacgcagga cacctcgttc gacgatcggc tcgcagaaag cgtcaaggga gccgtggccc
     1261 gaggcgtgca ggtcgggttc ggattcaagg cgttcgacgc cgcgacgaat cagcctcgga
     1321 tcgcgagcgc gatcgcgcgc gcgggcgccg cgctcggcgt cgtgtgcgtc ggcgacaggg
     1381 gaggcggcgg aaggaatgtc gcgtttgcca cgctcgcgct catccgaggc gaacgcgcgt
     1441 acccgggcct gccgctgatc gccgcctacg gatcggtcca gctccctggc ctcaccgccg
     1501 cctcgtccga ggtgcaggtg cccggcgcga gagcccccat ccccatctcg ttgaccgaga
     1561 ccttcgacga gtacgctcca ctctgtccgg cccgcgcggc ggggaccagc atggttcgct
     1621 tcattcccca cttgtcgagc cgtgaggcgc tccggcagcc gaccgtccgg cactccctga
     1681 tggacgtcgt gcgatccgcc ggccgcggtg acgccgtctt ccgcgccaag accgtactcg
     1741 tcggcgccga gcaagcgcgc gatattctcg acacaccgct cgacctggcc gggtctcgct
     1801 atggcttcga gttccaggcc gacgcggtca atgcgctcct caccgggcgg atcgtcacgc
     1861 cggtgaagcg gaccgggcag gtcatctcgg tcgtcggcgt ggccgcgttt gctgcgctcc
     1921 tctatctctc cggacccggc cgctcgcggc ggagactcgc ctgggtaccg tggtgcgtcg
     1981 cggtcctgag cttcgtcctc gccgtcctcg cataccggtt cctcgactcc ctgtggtcgc
     2041 ccgtctttcc tgctgccgct tgcctcgtga cctgggccgt tctcgggttc ctggatcgga
     2101 ggtggaccca tgacgaacgt tagagatcgc atgttcgact ctctcgttgt ccgagcgcgc
     2161 ctgtaccgct cggctcggat gcagcacccg caccgccgcg cagtcagctg cgcgctggtg
     2221 gcgctgacgc tcctcggatg cgagacagtg ggccctggca gatccggagg cgggtctgcc
     2281 cgggtggaga tcgtggcgcg gttgaccgcc acctctcccg gcgtccgcat cgagcgatcg
     2341 ggacaaccga tgccctatac gccgaacatg gtcgtgcggg aaggagaccg catccggacg
     2401 ggatccgaca cgtacgccgc gattgacttc gacgatggca acaccgtgta cctgaaccac
     2461 gacacctggg tcacgatcgg atcgattcgc gtgctgctgg gcgagatctt caacgcggtc
     2521 gtccgtgtcg gcgcgggatc cgaggtctac acaaacgacc tcaccgccgc cgccgagggc
     2581 acgaagttcc tactccgtat cgacccggtg agcacgac
//
LOCUS       DASTKC010000015         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1197247, whole genome shotgun sequence.
ACCESSION   DASTKC010000015 DASTKC010000000
VERSION     DASTKC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197247"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VFR25_00595"
     CDS             <1..178
                     /locus_tag="VFR25_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9939576.1"
                     /translation="DVTGVATLPEGREMVMPGDNVEMAVELITPIAMEKELRFAIREG
                     GRTVGAGVVAEVTE"
     gene            178..498
                     /gene="rpsJ"
                     /locus_tag="VFR25_00600"
     CDS             178..498
                     /gene="rpsJ"
                     /locus_tag="VFR25_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET9939577.1"
                     /translation="MAGTAPGQRIRIRLRSYDHAVLDQSAGEIVRTAKRTGARTAGPI
                     PLPTQRSVYTVLRSPHVDKKSREQFEVRVHKRLIDIVKSTPQTIDALMKLDLPAGVDI
                     EIKL"
     gene            514..1146
                     /gene="rplC"
                     /locus_tag="VFR25_00605"
     CDS             514..1146
                     /gene="rplC"
                     /locus_tag="VFR25_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET9939578.1"
                     /translation="MIGLLAQKVGMTQVYGEDGRVVPVTVLTAGPCPVVQIKTKERDG
                     YAALQIGFGTKRESLIRKPIQGHLKKHQASGVRMLRELRVDDASPFEVGQKLDVSMFE
                     VGSSVDVIGVSKGRGFAGVTKRHHFTGGRETHGCVTHKQPGSIGASAYPSRVIKGKRL
                     PGRMGGGRVTVKNLGVIGVDAEQNLIWIRGSVPGHPNCTVLIRQTAKARA"
     gene            1146..1775
                     /gene="rplD"
                     /locus_tag="VFR25_00610"
     CDS             1146..1775
                     /gene="rplD"
                     /locus_tag="VFR25_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET9939579.1"
                     /translation="MATARRFGPDGKEMAKVDLPAEIFEAPVNEHLIWEAVKIHLGNQ
                     RQGTAKAKNKAAVSGGGRKPWKQKGTGRARSGSNTSPLWPGGGVAFGPQPRDYTARMN
                     QKARRSALLGALTVRAKDGNVIIVDAPAMGEPKTKPLAQLLKTVGLEGRKVLWVFDKT
                     GDVLRKSSRNLDKVETSESRTLNTYELMHCECLVLTEDGLKSLTERLSA"
     gene            1772..2065
                     /gene="rplW"
                     /locus_tag="VFR25_00615"
     CDS             1772..2065
                     /gene="rplW"
                     /locus_tag="VFR25_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET9939580.1"
                     /translation="MKREPRTIVRRALITEKGTQIRERENGYLFEVARDANKIEIRQA
                     IESIFPVKVESVRTMRVHGKPKRMGRYAGHRPDWKKAIVMLKKGQTIELFEQV"
     gene            2069..2902
                     /gene="rplB"
                     /locus_tag="VFR25_00620"
     CDS             2069..2902
                     /gene="rplB"
                     /locus_tag="VFR25_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9939581.1"
                     /translation="MPLRKFRPLTPAQRFRTVASFDEITRSKPEPRLIEKLKKTGGRA
                     NTGHTSAWLRGGGHVKKYRIIDFKRDKTGIPATVAHIEYDPNRSARIALLHYKDGEKR
                     YIIAPQQLLVGHTVMSGADAEVRPGNAMPLGSVPLGTFVYNVELVPGKGGQMSRSAGT
                     YCQVMAREGQYAQLRLPSGEMRKVPVVCICSIGMVGNEDHQNIVIGKAGKSRWMGRRP
                     SVRGVAMNPVDHPMGGGEGKSSGGRHPCSPWGLLAKGKKTRKRKLSDALIVRRRKSKG
                     A"
     gene            2933..>3059
                     /locus_tag="VFR25_00625"
     CDS             2933..>3059
                     /locus_tag="VFR25_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HET9939582.1"
                     /translation="MSRSVKKGPFVDAKLLKRLEAMNRVNERRVLKTWARRSTISP"
BASE COUNT          585 a          948 c         1099 g          427 t
ORIGIN      
        1 ggacgtgacg ggtgtggcga cgttgcccga gggtcgggag atggtgatgc cgggggacaa
       61 cgtggagatg gcggtggagc tgatcacgcc gatcgcgatg gagaaggagc ttcgcttcgc
      121 catccgtgag ggcggccgca ccgtcggcgc cggcgtcgtc gcggaggtca ccgagtgatg
      181 gctggaaccg ctccagggca gcgcatccgc attcgtctgc ggtcgtacga ccacgccgtg
      241 ctcgaccagt cggcggggga gatcgtccgc acggcgaagc gaaccggcgc acggacggcg
      301 ggaccgatcc cgctgcccac gcagcgttcc gtgtacaccg tgctccggtc ccctcacgtc
      361 gacaagaagt cgcgggagca gttcgaggtc cgggtgcaca agcgtctcat cgacatcgtg
      421 aagtccacgc cgcagacgat cgacgcgctg atgaagctcg atctgcccgc gggcgtggat
      481 atcgaaatca agctctaggc ggacgggatg gccatgatcg ggttgctcgc acagaaggtc
      541 ggcatgaccc aggtgtacgg ggaggacgga cgcgtggttc cggtgaccgt gctcacggcc
      601 gggccgtgcc ccgtggtcca gatcaagacg aaggagcggg acggctacgc cgcgctccag
      661 atcggcttcg ggacgaagcg tgaatcgctc atccggaagc cgattcaggg ccacctgaag
      721 aagcatcagg cgtccggcgt ccgcatgctg cgcgagctgc gcgtggacga cgcctcgccc
      781 ttcgaggttg gccagaagct cgacgtctcg atgttcgagg tggggagctc ggtcgacgtg
      841 atcggcgtgt cgaagggacg cggctttgcc ggcgtcacca agcggcacca cttcacggga
      901 ggccgcgaga cgcacggctg cgtgacgcac aagcagccgg gctcgatcgg cgcttcggcg
      961 tatccgagcc gcgtcatcaa gggcaagcgc ctccccggcc gcatgggcgg cgggcgcgtg
     1021 acggtcaaga acctgggcgt gatcggcgtc gacgccgagc agaacctgat ctggattcgc
     1081 ggctcggtcc cgggccatcc gaactgcacg gtcctgatcc gccagacggc aaaggcgagg
     1141 gcgtaatggc gaccgcgcga cgcttcggac cggacggcaa ggagatggcc aaggtggacc
     1201 tcccggcgga gatcttcgag gcgccggtga acgagcattt gatctgggaa gccgtgaaga
     1261 tccacctcgg caaccagcgg cagggtacgg ccaaggcgaa gaacaaggcc gccgtgagcg
     1321 gtggcgggcg gaagccctgg aagcagaagg gcactggccg cgcgcgctcg ggctcgaaca
     1381 cctccccgct ctggccggga ggcggcgtcg cgttcgggcc gcagccgcgt gactacacgg
     1441 cgcgcatgaa ccagaaggct cgccgctcgg cgctcctagg agcgctcacg gtcagggcca
     1501 aggacggaaa cgtgatcatc gtggacgcgc cggccatggg cgagccgaag acgaagcccc
     1561 tggcgcagct cttgaagacg gtcggcctcg aaggacggaa ggtgctctgg gtgttcgaca
     1621 agacgggcga tgtgcttcgc aagtcgtccc ggaacctcga caaggtcgag acctccgaga
     1681 gcaggaccct caacacgtac gagctgatgc actgcgagtg tctggtcctg accgaggatg
     1741 gcctcaagag cctgacggag cgactgagcg catgaaacga gagccgcgca ccatcgtgag
     1801 acgcgccctg atcaccgaga agggcaccca gattcgcgag cgcgagaacg gatacctgtt
     1861 cgaggtggcg cgcgacgcga acaagatcga gatccgccaa gcgatcgagt cgatcttccc
     1921 cgtgaaggtc gagtcggttc gcaccatgcg cgtgcacggc aagcccaagc ggatgggccg
     1981 ctacgccgga caccgccccg actggaagaa ggcgatcgtc atgctcaaga aggggcagac
     2041 gatcgagctc ttcgagcagg tatagccgat gccacttcgc aaattcaggc cgctcacgcc
     2101 ggcccagcgt ttccggaccg tcgcgtcgtt cgacgagatc acgcggtcga agcccgagcc
     2161 gcgcctcatc gagaagctga agaagacggg cggccgcgcc aacacggggc acacgtccgc
     2221 gtggcttcgg ggcggcgggc acgtcaagaa gtaccggatc atcgatttca aacgcgacaa
     2281 gacgggcatt cccgcgacgg tcgcgcacat cgagtacgac ccgaaccgaa gcgcccgcat
     2341 cgccctcctc cattacaagg acggcgagaa gcgctacatc atcgccccgc agcagctcct
     2401 ggtcgggcac acggtgatgt cgggggcgga cgccgaggtg cgtccgggga acgcgatgcc
     2461 gctcggatcg gtgccgctcg gcacgttcgt ctacaacgtg gagctggtgc ccggtaaggg
     2521 cggccagatg agccggagcg cgggcaccta ctgccaggtc atggcgcgcg agggccagta
     2581 cgcgcagctt cggctcccgt cgggcgagat gcgcaaggtg ccggtcgtct gcatctgctc
     2641 gatcggcatg gtgggcaacg aggatcacca gaacatcgtg atcggcaagg cgggcaagtc
     2701 ccgctggatg ggccggcgtc ccagcgtgcg cggcgtggcg atgaaccccg tcgatcaccc
     2761 gatgggcggc ggcgaaggca agtcctcggg cgggcggcat ccgtgctcgc cgtgggggct
     2821 tctggccaag ggcaagaaga cgcgcaagcg gaagctgtcg gacgcgctga tcgtgcgccg
     2881 gcggaagtcg aagggcgcgt aggcagctaa cgaaacagag ggcaatgacg cgatgagccg
     2941 ttccgtcaaa aagggtccct tcgtggacgc caagcttctg aagcgcctcg aggcgatgaa
     3001 ccgggtgaac gagcgccgtg tgctcaagac ctgggcacgc cgctcgacga tctctccgg
//
LOCUS       DASTKC010000016         6023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1908817, whole genome shotgun sequence.
ACCESSION   DASTKC010000016 DASTKC010000000
VERSION     DASTKC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908817"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFR25_00630"
     CDS             complement(<1..363)
                     /locus_tag="VFR25_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET9939583.1"
                     /translation="MLPLTGADDATIRRVMADEGLRLAPGEVRRMLELLERDPTRVEA
                     TIFDTMWSEHCSYKSSRWALKAFLPTESPDVILGPGEDAGVVRLGTHAGVSYALVIAH
                     ESHNHPSQVVPVEGAATGI"
     gene            complement(371..1684)
                     /gene="purB"
                     /locus_tag="VFR25_00635"
     CDS             complement(371..1684)
                     /gene="purB"
                     /locus_tag="VFR25_00635"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9939584.1"
                     /translation="MIERYSRPAMAALFDDAARYRTWLEVEIAAAEAMERKGDVPRGA
                     TARIKERAKVDAKRVDELERTLRHDVIAFLTQVGETVGEDARHLHLGMTSSDLVDTAL
                     ALTLVRATDLLTREVEALRQAARDLAVRTRDLPMVGRTHGIHAEPITFGLKVLVWYEE
                     LGRALDRLARAREGIAVGKLSGAVGTHAHLSPELEEEVLGSLGLKPEPVSTQVVQRDR
                     HAELLAAVAILGASLEKIALEIRHLQRTEVLEAEEPFREGQKGSSAMPHKRNPVNCER
                     VCGLARILRANAHAALENVALWHERDISHSSVERVILPDSFLLADFMAAQMKEIVSDL
                     RIYPDRMRANLDLTRGLVYSQRVLLALTTAGMSREAAYAAVQGHAMEAWRGGPDLKSR
                     LLADPAVTKALPRADLEACFEPAHYLRHVERIFDRVLGTPVAAGH"
     gene            complement(1713..2960)
                     /locus_tag="VFR25_00640"
     CDS             complement(1713..2960)
                     /locus_tag="VFR25_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9939585.1"
                     /translation="MSQPEVETPVRRWTGTLAPLSAGVLMGLVVLVGLLVLAPAKHRA
                     GLADTENAPAAAAKPAATPAVTAAQVPAAIDNSRRTAIVEAAERVGPAVVTLSVVQTR
                     VVSTAPGDFFEPFFRDMIPQYRYREQIPSMGSGFIISKDGYVLTNEHVVHGADKITAI
                     LPDGRNLTGRLVGAHPQYDLAIVKIDGKNLPTAPLGTSNDLMVGEWAIAIGNPFGFLL
                     NDTQPTVTAGVVSATRRDIKAQTQSAGIYKNMIQTDAAINPGNSGGPLVNAKGEVIGV
                     NTFIFTKSGGSEGIGFAIPIDAAKHVVDEIIKYGRVRNVWIGVATWELTPYVAERLQT
                     TDRKGLYVATVDKGSPADRAGVRVGDIIRKVNGTEVSDGNEAYRVIFGAGVGDTIGLT
                     LEREGKLFTVKLLLAEAPDGGGE"
     gene            complement(2947..3741)
                     /gene="truA"
                     /locus_tag="VFR25_00645"
     CDS             complement(2947..3741)
                     /gene="truA"
                     /locus_tag="VFR25_00645"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9939586.1"
                     /translation="MASERRVAAFRLGVEYDGTGFHGWQVQRSHRTVQGDLEAALKRL
                     AGEPVRVTGAGRTDSGCHAAGQVASVTLKTRLDAAGLKTALNALLSPDVRVVEAAEVS
                     AAFHARFDATRRAYRYLIEPRASALLARYAWLRRVRAGVEALNEASRPLLGEHDFTSF
                     SRQGSDADERPVCTVVRARWKRLHGRMRFDIESDRYLYTMVRRIVSSVIRAAENGAGA
                     AQVRAILAGRDRRLAAPPAPAHGLYLMRVKYPRVGWIPKEPLDVPA"
     gene            complement(3731..4537)
                     /locus_tag="VFR25_00650"
     CDS             complement(3731..4537)
                     /locus_tag="VFR25_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939587.1"
                     /translation="MTLQVGCRTSALRLQAQRKRNPVPGSPETDRLTPSRSTTRAVHP
                     AALLLACLAAGLGIALTSSWPALALAAALFLTLTLRAEHRTLAAETPFLAIALIVFAA
                     HTLLSGVPVRQAIEPAAKIALRLLALLYLLRWAARTFLGGAARWALSLELPRRPRWLL
                     HALESGRHALTLTPRAIREAEHQHMALRARGLRSGKGFGGRGRYLAAWVLPFLGTMLR
                     LGESYEEALLARGYVLGAARKSGQVLAWGLPEVMVLALGALTAAALLRGV"
     gene            complement(4569..>6023)
                     /gene="lnt"
                     /locus_tag="VFR25_00655"
     CDS             complement(4569..>6023)
                     /gene="lnt"
                     /locus_tag="VFR25_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET9939588.1"
                     /translation="AQGTPGPAEPGRTARGVRWSPWIAGIVFNVLMFWWIVRLPVHAM
                     THPWIIWPALAALGLYLGLYFALFGWAVRFLRRRLGVSILIVAPFVWTVIEWAKSSGP
                     LGCPWGNLGYALAREPAWIQGASIAGAPGLTFWIVSVNALVAGIIVARRWPTRVACAV
                     LALAALVLPVRWGYDRLKTAPTAPLTRVALVQPNTPSDVKWNTEAQDSVVGALFDGAR
                     EAAAATPRPDLVIWPETSLPFYVRLEPAKLLRFLGLVKEIGIPILAGYPDARLSTGGT
                     ALTYNAAGLVRPNGTFAAQYEKMHLVPFGERIPFQEVMPFLGKIDLGQAEWTPGTDPV
                     VFGGGGATFGVMICFESIFPDHARTLALEGAQYLVNITNDEWFGKTAGPVQHADMAIL
                     RSVELGRATARCANTGISMFVDPYGRVSGATPLFERAIAEGVVPAPLSHTLWLEWGDW
                     ITMVSIAVTAILLAVGWFRPIQRLDAPHEAREWR"
BASE COUNT          936 a         2111 c         2087 g          889 t
ORIGIN      
        1 gatccccgtc gcggcgccct cgaccggcac cacctgcgat ggatggttgt ggctctcgtg
       61 cgcgatgacg agcgcgtacg agacgccggc gtgcgtgccc agccggacca cgcccgcgtc
      121 ctcgccgggg ccgaggatga cgtcggggct ctccgtagga aggaaggcct tgagcgccca
      181 ccggctcgac ttgtacgagc agtgctcgct ccacatggtg tcgaagatcg tcgcctcgac
      241 acgggtgggg tcgcgctcca ggagctcgag catccgtcgc acttcgcccg gcgcgaggcg
      301 gagcccctcg tcggccatga cgcggcgaat cgtcgcgtcg tccgccccgg tcagggggag
      361 cacggaggtg tcagtggccg gcggcaacgg gcgttcccag gacgcgatcg aagatccgct
      421 ccacgtggcg gaggtaatgg gcgggctcga agcacgcttc gagatccgcg cgagggagcg
      481 ccttggtgac ggcgggatcg gcgaggaggc gcgacttgag atcggggccg ccccgccagg
      541 cctccatcgc gtgcccttga accgcggcgt acgccgcctc ccgcgacatc cccgcggtgg
      601 tgagcgcgag gaggaccctc tgcgagtaga cgaggccgcg ggtcagatcg agattggcgc
      661 gcatccggtc gggatagatc cggaggtccg acacgatctc cttcatctgc gcggccatga
      721 agtccgccag caggaacgaa tcgggcagga tcacccgctc cacgctcgag tggctgatgt
      781 cccgctcgtg ccagagcgcc acgttctcga gcgccgcgtg cgcgttcgcg cggagaatgc
      841 gcgcgagtcc gcaaacgcgc tcgcagttca ccggattccg cttgtgcggc atcgccgagg
      901 atcccttctg cccttcgcgg aacggctcct cggcctcgag cacttcggtg cgctgcagat
      961 ggcggatctc gagcgcgatc ttctcgagcg acgcgccgag gatggcgacg gcggcgagga
     1021 gctccgcatg gcggtctcgc tgcacgacct gggtcgagac gggctcgggc ttcaatccga
     1081 gcgatcccag cacctcttcc tcgagctcgg ggctcaggtg cgcgtgcgtg ccgaccgccc
     1141 ccgacagctt cccgaccgcg atcccctctc gcgcccgggc gaggcggtcc agagcgcgtc
     1201 ccagctcctc gtaccagacc aagactttga gtccgaacgt gatcggctcg gcgtggatgc
     1261 cgtgcgtgcg ccccaccatc ggaaggtcgc gcgtgcgcac cgcgagatcg cgcgcggcct
     1321 ggcggagggc ttcgacttcg cgcgtgagaa gatccgtcgc gcggacgagc gtcaaggcga
     1381 gcgccgtgtc gacgaggtcg gaggaggtca ttccgagatg gagatggcgg gcgtcctcgc
     1441 cgaccgtctc ccccacctgg gtcaggaagg cgatcacgtc gtggcggagc gtccgctcca
     1501 gctcgtcgac gcgcttcgcg tcgaccttgg cgcgctcctt gatgcgggcc gttgcgccgc
     1561 gcggcacgtc ccccttccgc tccatcgcct cggcggcggc gatctccacc tcgagccacg
     1621 tgcggtagcg ggcggcgtca tcgaacagcg cggccatcgc cggccgggaa taccgttcga
     1681 tcatcggacc gtcccccctt ccggtcgcag cgtcattcgc ctcccccgtc cggagcttcc
     1741 gcgaggagga gcttcacggt gaagagcttc ccctcccgct cgagcgtgag cccgatcgtg
     1801 tccccgacgc cggcgccgaa aatcacacga tacgcttcgt tcccgtcgct cacctcggtt
     1861 ccattgacct tgcggatgat gtcccccacg cggacccccg ccctgtccgc ggggcttccc
     1921 ttgtcgaccg tcgccacgta gagccccttc cggtccgtcg tctggagccg ttcggctacg
     1981 tacggcgtga gctcccacgt ggccactccg atccagacgt tccgcacccg gccgtacttg
     2041 atgatctcgt cgaccacgtg cttggccgcg tcgatcggaa tggcgaatcc gatcccctcg
     2101 gagccgccgc tcttcgtgaa gatgaaggtg ttgacgccga tcacctctcc cttcgcgttc
     2161 acgagcggtc cgccggagtt gcccggattg atggcggcgt cggtctggat catgttcttg
     2221 tagatgcctg cgctctgcgt ctgcgccttg atgtcgcggc gcgtggcgct gaccacgccg
     2281 gccgtgacgg tgggttgcgt gtcgttgagg aggaagccga agggattccc gatcgcgatg
     2341 gcccactcgc cgaccatcag gtcgttcgag gttccgagcg gcgcggtcgg aaggttcttg
     2401 ccgtcgatct tgacgatggc caggtcgtac tgcggatgcg ctccgacgag ccggccggtc
     2461 aggttgcggc cgtccggcag gatcgcggtg atcttgtcgg cgccgtgcac cacgtgctcg
     2521 ttcgtgagca cgtagccgtc cttcgagatg atgaagcccg aacccatgct cgggatctgc
     2581 tcgcggtagc gatactgcgg gatcatgtcg cggaagaagg gctcgaagaa gtcgccgggc
     2641 gccgtggaca cgacgcgcgt ctggaccacg ctcagcgtca ccacggcggg gccgacccgc
     2701 tcggcggctt ccacgatcgc ggtgcggcgg ctgttgtcga tggcggccgg tacttgagcg
     2761 gcggtcacgg ccggagtggc ggccggcttc gcggccgcgg ccggtgcgtt ctccgtatcg
     2821 gcgaggccgg ccctgtgctt cgcgggggcg agcacgagga gaccgaccag gacgacgagg
     2881 cccatcagca ccccggccga aagcggggcc aacgtcccgg tccagcggcg gaccggcgtc
     2941 tctacttcag gctgggacat cgagaggctc cttcgggatc catcccacgc gtggatattt
     3001 cactcgcatc agatacaacc cgtgcgccgg cgcgggcggc gccgcgagac gccggtcgcg
     3061 ccccgcgagg atcgcccgca cctgggcggc gccggcaccg ttctcggccg cgcgaatcac
     3121 gctcgacacg atgcggcgca ccatcgtgta gaggtaccgg tcggattcga tgtcgaagcg
     3181 catccgtcca tgcaggcgct tccatcgcgc gcggacgacc gtgcagaccg gccgctcatc
     3241 cgcgtcggac ccctgccggg agaacgacgt gaagtcgtgc tcccccaaaa gcgggcgcga
     3301 cgcctcgttc agcgcctcca cgcccgcgcg cactcgtctc agccaggcgt accgcgcgag
     3361 cagcgccgag gcgcggggct cgatgagata gcgatacgcg cgccgcgtgg cgtcgaagcg
     3421 cgcgtggaac gccgcgctga cctccgccgc ctcgaccaca cgtacatccg gcgacaggag
     3481 cgcgttgagc gccgtcttga gtcccgcggc atcgagccgc gtcttcaagg tgacgctcgc
     3541 cacctgaccc gccgcgtggc agcccgagtc cgtccggcct gctcccgtca cgcgcaccgg
     3601 ctcccccgcg agccgcttga gcgccgcctc gagatcaccc tgcacggtgc gatgcgaccg
     3661 ctggacctgc catccgtgaa acccggtgcc gtcgtattcc acccccaggc ggaacgccgc
     3721 aactcgccgc tcagacgcca cgcaggagcg ccgcggccgt gagcgcgccc agagccagga
     3781 ccatgacttc cggaagaccc caggcgagga cttgcccgct ctttcgggcc gccccgagaa
     3841 cgtaaccccg ggccaggaga gcctcttcat aggattcccc cagccgaagc atggttccga
     3901 ggaacgggag gacccaggcc gcgaggtacc tgccccgccc gccgaagccc ttgccggagc
     3961 gcaacccacg ggcgcgcaag gccatgtgct ggtgctccgc ctcccggatc gcccggggcg
     4021 tcagggtcag agcgtgccga ccgctctcca gggcgtggag gagccaccgg gggcggcgag
     4081 ggagctcgag cgagagggcc caccgggccg cacctcccag gaacgtccgg gcagcccatc
     4141 gcagcaagta gaggagcgct aggaggcgga gcgcgatctt ggccgcgggc tcgatcgcct
     4201 gccgaaccgg aacgcccgac aggagcgtgt gcgccgcgaa gacgatcagc gcgatcgcga
     4261 ggaacggcgt ctccgccgca agcgtgcggt gctccgcccg gagcgtgagc gtcaggaaga
     4321 gggctgcggc caaggcgagc gcgggccagg agctggtgag ggcgattccg agaccggcgg
     4381 cgagacaggc gaggaggagc gccgcgggat ggaccgcacg cgtcgtactg cgagatggcg
     4441 tgagacgatc ggtctcggga gagccgggca ccggattgcg tttcctctgg gcctggaggc
     4501 gaagcgccga cgtccggcac cccacctgaa gcgtcactct agccgcttcc gcggcccctg
     4561 tcaacgcttc agcgccactc ccgcgcctcg tgcggcgcgt ccagccgctg gatcggacgg
     4621 aaccagccca cggcgagcag gatcgcggtc accgcgatcg agaccatcgt gatccagtcg
     4681 ccccactcca gccagagcgt gtgcgagagc ggcgccggca cgactccctc cgcgatcgcg
     4741 cgctcgaaca gaggcgtcgc gccggagacg cggccgtagg gatccacgaa catcgagatc
     4801 cccgtgttgg cgcaccgcgc cgtggcgcgt cccagttcca ccgacctcaa gatcgccatg
     4861 tccgcgtgct ggacgggacc ggccgtcttc ccgaaccact cgtcattcgt gatgttgacg
     4921 aggtactggg ctccttccag cgcgagcgtg cgcgcgtggt ccgggaagat cgactcgaag
     4981 cagatcatca cgccgaacgt cgcgccgcca ccaccgaaca cgaccggatc ggttccgggc
     5041 gtccactccg cctgcccgag gtcgatcttg ccgaggaacg gcatcacttc ctggaacgga
     5101 atccgctctc cgaacgggac caggtgcatc ttctcgtact gcgccgcgaa ggtgccgttg
     5161 gggcgcacca gcccggcggc gttgtacgtg agcgcggtgc cacccgtgga aaggcgcgcg
     5221 tcgggatagc ccgcgaggat cggaatgccg atctccttca cgagtccgag gaatcgaagg
     5281 agcttggcgg gctcgagccg gacatagaac ggcagcgagg tctcgggcca gatgacgaga
     5341 tcggggcgag gcgtcgcggc ggcggcctca cgcgctccgt cgaagagagc gccgacgacc
     5401 gaatcctgcg cctctgtatt ccatttgacg tcgcttggcg tgttcggttg gacgagcgcc
     5461 acgcgcgtga gcggcgctgt gggtgcggtc ttcaggcgat cgtagcccca tcgaaccggc
     5521 aggacgagtg ccgcgagcgc gaggacggcg cacgcgacgc gggtcggcca tcgccgcgcg
     5581 acgatgatcc cggcgacgag cgcgttcacc gacacgatcc agaacgtgag ccccggcgcg
     5641 cccgcaatgg atgctccctg gatccaggcc ggctcgcgcg cgagcgcgta cccgagattg
     5701 ccccacgggc agccgagcgg ccccgaggat ttcgcccact cgatcacggt ccagacgaac
     5761 ggcgcgacga tgaggatgga gaccccgagc cggcgtctga ggaagcgaac cgcccacccg
     5821 aagagcgcga agtagaggcc caggtagagt ccgagcgcgg cgagtgcggg ccagatgatc
     5881 caagggtggg tcatggcgtg gaccggcagc cgcacgatcc accagaacat gagcacgttg
     5941 aagacgatcc ccgcgatcca cggggaccac cgcacgccgc gcgccgtgcg tcccggctcg
     6001 gccggccccg gagttccctg ggc
//
LOCUS       DASTKC010000017         5500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1909134, whole genome shotgun sequence.
ACCESSION   DASTKC010000017 DASTKC010000000
VERSION     DASTKC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909134"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFR25_00660"
     CDS             <1..511
                     /locus_tag="VFR25_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9939589.1"
                     /translation="KASFPKEFAHLFEAQFPRLARYLNRLTGDLDLASDLAQEAFVRL
                     YQRGSMPDQPEAWLVSVAMNLLRTSASTRSRRLRLLTDARAEATLSDPPRAPDEGMEG
                     IEERERVRAALERMPERERRMLLLQAEGYTYRDIATAVRIHEASVGTLLVRARQTFRR
                     HYEDLFGAS"
     gene            501..1265
                     /locus_tag="VFR25_00665"
     CDS             501..1265
                     /locus_tag="VFR25_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939590.1"
                     /translation="MHLDDPRIQELLDGELEAREESAARAHLEDCVPSSGRFEEARSE
                     KVEIAALLETLDAPLPRIDVATIVARADATPRRARARFATLRRAAAVLLVAGLAGAVY
                     ALPGSPVRIWVHDVVRKMAPKPQSPVEPSPPRLEAGISILPEERLVILFRLGAAHGSG
                     VVSLALTGGSEVQVHAPPGAATFSSRPGLLEIEVRDVSAPFVVQVPRSAPWVEVRADE
                     APLFLKEGSRVTTAGPAESGDRYTLRLERRSPASAP"
     gene            1355..2122
                     /locus_tag="VFR25_00670"
     CDS             1355..2122
                     /locus_tag="VFR25_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939591.1"
                     /translation="MRVRSSFAYAAIPIALTLVLIGCSQDGQRGILEPSHSEALATAG
                     GAAEARVVIPFAAANFVGAVQNPFLPLVPGTILTYRQETPEGVEINTVEVTNRTKVIL
                     GVTTYVVHDQVFLEGALSEDTFDWMAPDKDGNVWYFGEDTKELGPPVSTQGSWVAGQN
                     GAMPGILMLAHPKVGNSYVQEDAPGVVADQGKVMDLNATVTVPYGTFTNCIKTQESTP
                     IEPGDRAFKVYAEGIGTVVEIPNKSAGRVELIAVQRP"
     gene            2122..2970
                     /locus_tag="VFR25_00675"
     CDS             2122..2970
                     /locus_tag="VFR25_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939592.1"
                     /translation="MRLSLSSTAFFLAAASALAPVGAGAEELPGYLRDRGTGVATSLF
                     GTYVRGGELLVYPFYEYTVNKDQEYSPEELGFVGTQDYRGKNTEHEALIFLSYGLSEN
                     VAVELESALWTTATQHKSPDDPSAMPSEVTESGFGDTQAELRWRWWKESESRPEFWSY
                     FETVFPFQKNRKLIGTQNWELIQGFGLTRGFHFGTLTTRASMEYQADEGKVAFGEYAL
                     EYLKRLSPKWRTVLAVEGEEDEVAAIVEVQRQLAPNAVLKLNNGFGLTDKAPDLAPEV
                     GIVFSW"
     gene            3047..5026
                     /gene="ftsH"
                     /locus_tag="VFR25_00680"
     CDS             3047..5026
                     /gene="ftsH"
                     /locus_tag="VFR25_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9939593.1"
                     /translation="MRQDSRPPTRGQDQPQRGQGPPPTPPPAPPRRIWLFFVLIVLAN
                     ILLTRMFFPSTRPVKVPYTLFKQEVVRGNVDKIYSRGESMTGRFRHAVIYPAKPDSAT
                     GQQPRSALDFSTTLPAFVDPGLESLLISHGVEISAEPIGQGNPWLGLLFSFAPALLLI
                     GLYVWAFRRAAKQGGLGGGLMGIGRSTARRFDSETADRVTFEDVAGIDEAENELVEIV
                     DFLKDPKKYQRLGGTAPKGVLLVGAPGTGKTLLARAVAGEAGVPFFSMSGSEFVEMIV
                     GVGAARVRDLFRLARESAPAIVFIDELDSIGRARGLVSIGGSSEQEQTLNQILTEMDG
                     FSSREGIIVLGATNQPDILDRALLRPGRFDRRVVVNLPDRKGREEILRVHTRRVPLDQ
                     DVQLGEIASATPGLAGADLRNLVNEAALLAARREETEVHAKDFFDALEKVVLGPERPI
                     LLSRDDRERIAYHEGGHAILGLVVPGADTVHRVSIVPRGMALGVTYQRPESDRYNYPE
                     SYLRARLIGMLGGRAAEEIVFGTRTTGAQNDIEQATHLARNMVTRWGMSDKLGMVQLA
                     PPENPYLAGPDGFGGPKGISEETASLVDAEVLRIIRESHDEAKRLLEVHRGALDKLAD
                     ALLSHETLDEREILEVTGLTPPPAPDGRPRRADGG"
     gene            complement(5043..>5500)
                     /locus_tag="VFR25_00685"
     CDS             complement(5043..>5500)
                     /locus_tag="VFR25_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939594.1"
                     /translation="AKTVGFRYHGHLNAWQCSEGTYLRHTETDRHARLVRFRAEAKVC
                     NDCRIKHHCTDSDEGRELIHPLDGWSRSDMARFQRALSVSLIVLASLFCAIETVRQSA
                     PPLRSFFLCGFLVGIVLARRELVRLKEAGPAGAHAAGSGVESGPGFPNG"
BASE COUNT          963 a         1838 c         1817 g          882 t
ORIGIN      
        1 gaaggcctcc tttccgaagg aattcgccca cctcttcgag gcccagtttc cgcggctcgc
       61 tcggtacttg aaccggctca cgggcgacct cgacctggcg tccgatctgg cgcaggaggc
      121 attcgtcagg ctctaccagc ggggatccat gcccgatcag ccggaggcgt ggctcgtgag
      181 cgtggcgatg aaccttcttc ggacgtccgc ctcgacgcgc tcgcgccggc tgaggctcct
      241 gacggatgcg cgagccgaag ccactctctc ggacccacct cgcgccccgg atgaaggcat
      301 ggagggaatc gaggagcggg agcgcgtgcg cgccgcgctc gagcggatgc cggagcgtga
      361 gcggcggatg ctgctgctcc aggccgaagg ctacacgtac cgcgacatcg ccacggcggt
      421 tcgcattcac gaggccagcg tcggcacact cctcgtaaga gcgcgccaga cgttccggcg
      481 acactatgag gatctgttcg gtgcatcttg acgatccacg catccaggag cttctcgacg
      541 gagagctcga ggctcgggag gagagcgcag cccgggctca tctcgaggac tgcgtcccta
      601 gcagcggacg cttcgaggaa gcgcggtcgg agaaggtcga gatcgcggca ctcctcgaga
      661 ctctggacgc gccgttgccg cggatcgacg tggccacgat cgtagcgagg gcggacgcaa
      721 ccccgcggcg ggcgcgtgcc cgcttcgcca ccctccgcag ggcggcagcc gtgctcctcg
      781 tggccgggct ggccggggcc gtctatgcgc tcccgggctc gcccgtgcgg atctgggtcc
      841 acgacgtggt gcgaaaaatg gctccgaagc cgcaatctcc cgtcgagcct tcgccgcctc
      901 ggctggaagc aggcatctcg attcttccgg aggagcgtct ggtcattctc ttccgtctcg
      961 gtgcggcgca cggaagcggc gtggtctcat tagcgctcac gggcggatcg gaggttcagg
     1021 ttcacgcgcc tcccggagcc gcgacgttct cctcccgccc ggggctcctc gaaatcgagg
     1081 tgcgggacgt ctcggcgccc ttcgtggtgc aggtgccgcg atccgcgccg tgggtcgagg
     1141 tgcgggcgga cgaggcacct ctgttcctca aggagggctc ccgcgtcacg acggccggcc
     1201 ccgcggaatc gggcgaccgc tacacgctcc ggctggagcg ccgtagcccg gcatcggccc
     1261 cctagcttcg cgcgtcacaa tcgcccggct cgcgggttcc ctctacggat agcagcgccg
     1321 acccggacac ctgtcgaaac gagaggagac catcatgcgc gtacgatcca gcttcgccta
     1381 cgcagccatc ccaatcgccc tcacgcttgt cctgatcgga tgcagccagg acggacagcg
     1441 gggcatcctc gagccgtccc actcggaagc tctcgccacg gcgggagggg ccgccgaggc
     1501 ccgcgtcgtg atcccattcg ccgccgcgaa tttcgtgggg gcagtccaga acccgttcct
     1561 tccgctcgtt ccgggaacca tcctcaccta ccgacaggag acgcccgagg gggttgagat
     1621 caacacggtc gaggtgacga acagaacgaa ggtgatcctt ggagtcacga cctacgtcgt
     1681 ccacgaccag gtgttcctcg aaggagcctt gtcggaagac acctttgact ggatggcgcc
     1741 cgacaaggac gggaacgtct ggtacttcgg cgaggacacg aaggagctcg gtcctcccgt
     1801 gagcacccag gggtcctggg tggcgggcca gaacggcgcg atgccgggca tcctgatgtt
     1861 ggctcacccc aaggttggca attcctacgt tcaggaagac gctccgggcg tcgtcgccga
     1921 tcaaggcaag gtcatggacc tgaacgctac ggtcaccgtg ccctacggca cgttcacgaa
     1981 ttgcatcaag acccaggagt cgacgccgat cgagccgggt gatcgcgcct tcaaggtcta
     2041 tgccgaagga atcggaacgg tggtcgagat ccccaacaag agcgccggcc gcgtcgagct
     2101 gatcgcggtg cagaggccct agtgcggctt tccctttcga gtaccgcgtt cttcctcgcg
     2161 gccgcgtcgg cgctcgcccc cgtgggcgct ggcgcggaag agctcccggg atatctccgg
     2221 gatcgaggca ccggcgtcgc cacctcgctc ttcggaacgt atgtgcgcgg gggcgagctc
     2281 ctcgtctatc ccttctacga atacacggtg aacaaggatc aggaatactc acccgaagag
     2341 ttgggcttcg tgggcacgca ggactaccga ggaaagaaca cggagcacga ggcactcatc
     2401 ttcctgtcgt atggattgtc cgagaatgtc gcggtcgagc tggaatccgc gctctggacg
     2461 accgccacgc agcacaaatc gccggacgat ccatcggcca tgcccagtga ggtcaccgaa
     2521 tcgggattcg gagacaccca ggcggagctg cgctggcgct ggtggaagga gtccgaaagc
     2581 cgacccgaat tctggagcta tttcgaaacc gtgttcccgt tccagaagaa ccgcaagctg
     2641 atcggaaccc agaactggga gctgatccag ggattcggac tgacccgcgg atttcatttc
     2701 ggcacgctca cgacgcgcgc atcgatggag taccaagcgg acgaaggtaa ggtcgcgttc
     2761 ggcgagtacg cgctcgagta cttgaagcgc ctctccccga aatggcgcac ggtcctggcc
     2821 gtcgagggtg aggaggacga ggtggccgcg atcgtcgagg tgcagcggca gcttgcgccg
     2881 aacgcggtgc tcaagttgaa caacgggttc ggcctcacgg acaaggctcc ggatttggcg
     2941 cccgaggtcg ggatcgtgtt ctcctggtag ggaccggccc ccgctcgatc gcgccaccgc
     3001 cacgcccagc gccatgtaaa ctgaagggat ggcgcccgac cggcccgtga gacaggactc
     3061 tcgccctccc acccgggggc aggaccagcc ccagcggggc cagggaccgc ctcccacgcc
     3121 tccaccggca cccccgcgcc ggatctggct cttcttcgtc ctcatcgttc tcgcgaacat
     3181 cctcctcacg cgcatgttct tccccagcac gcgcccggtg aaggtcccct acacgctctt
     3241 caagcaggag gtcgtccgag ggaacgtcga caagatctac agccgcggcg agagcatgac
     3301 ggggcggttc cgccatgcgg tcatctatcc ggccaaaccg gactcagcga ccggacagca
     3361 gccgcgctcg gcgctggact tctccaccac gctccccgcc ttcgtcgacc ccggtctcga
     3421 atcccttttg atctctcacg gcgtggagat cagcgccgag ccgatcggcc aggggaatcc
     3481 ctggctcgga ctcctgttca gtttcgcccc cgcgctcctc ctcatcgggc tctacgtgtg
     3541 ggcgttccgc cgcgcggcca aacagggcgg gctggggggc ggtctcatgg gaatcggtag
     3601 gagcacggcg cgtcgattcg acagtgaaac ggcggaccgg gtcacgttcg aggacgtcgc
     3661 aggcatcgac gaggccgaga acgagctcgt cgagatcgtc gacttcctca aggatcccaa
     3721 gaagtatcag cgcctcggag gcaccgcgcc gaaaggcgtg ctcctggtcg gcgcgcccgg
     3781 caccggcaag acgctcctcg cgcgcgccgt cgcgggcgag gcgggtgttc ccttcttctc
     3841 catgagcgga tccgagttcg tcgagatgat cgtcggcgtg ggcgccgcgc gcgtgcggga
     3901 cctcttccgc ctggcgcgcg agagcgctcc ggccatcgtg ttcatcgacg agctggactc
     3961 gatcggacgg gcgcgcggac tcgtgtcgat cggcggatcg agcgagcagg agcagacgct
     4021 caatcaaatc ctcacggaga tggacgggtt ctcgagccgc gagggcatca tcgtgctcgg
     4081 cgcgaccaat caacccgaca ttctcgaccg cgcccttctc cgccccgggc gtttcgaccg
     4141 gcgcgtcgtg gtgaacctcc ccgatcggaa ggggcgcgag gagatcctgc gcgtccacac
     4201 gcgccgcgtg cctctcgatc aggacgtgca gctgggagag atcgcctccg ccacgcctgg
     4261 cctggccggc gcggacctac ggaacctcgt caacgaggcc gcgcttctcg cggccaggcg
     4321 cgaagagacc gaagtccacg cgaaggactt cttcgatgcg ctggagaagg tggtgctggg
     4381 tcccgagcgg cccattctcc tgagccgcga cgatcgcgag cggatcgcct atcacgaggg
     4441 agggcacgcg atcctcggtc tggtggtacc gggcgcggat accgtccatc gcgtctcgat
     4501 cgtgccgcgc ggcatggctc tgggcgtcac ctaccagcgc cctgagagtg atcgctacaa
     4561 ctacccggag tcctacctgc gcgcgcgtct catcggaatg ctcggcggac gcgcggcgga
     4621 agagatcgtc ttcggtaccc gcaccacggg cgcgcagaac gacatcgagc aggccacgca
     4681 cctcgcacga aacatggtca cccgctgggg catgagcgac aagctgggca tggtgcagct
     4741 cgcgccgccc gagaatccct acctcgcggg acctgatgga ttcgggggtc caaaggggat
     4801 cagcgaggag acggcgagcc tcgtggatgc ggaggtgctc cggatcatcc gcgagagcca
     4861 cgacgaggcg aagcgcctgc tcgaggtgca tcgcggcgcg ctcgacaagc tcgcggatgc
     4921 gctgctatca cacgagacgc tcgatgagcg cgagatcctc gaggtgaccg gactcacgcc
     4981 accgccggcg ccggacggaa ggccgcgtcg cgcggacgga ggctgaggta gatcccttta
     5041 gcttagccgt taggaaagcc cggacccgac tcgactcccg atccggcggc atgcgcaccg
     5101 gcgggcccgg cttccttgag tctcaccagc tcacgcctcg ccagaacgat ccccaccaga
     5161 aacccacaca ggaagaacga tcgtagcgga ggcgcggatt gccgcaccgt ctcgatcgcg
     5221 cagaagaggg atgcgagcac gatcaaactg accgagagag ccctctggaa gcgggccata
     5281 tccgatcggg accacccatc gagcggatgg atcagctccc tcccctcgtc ggagtcagtg
     5341 cagtgatgct tgatgcggca gtcgttacag accttggcct cggcgcggaa gcggaccagg
     5401 cgggcgtggc gatcggtttc cgtgtgccgg agataggtgc cctccgagca ctgccacgcg
     5461 tttagatggc cgtggtaacg aaacccaacc gtctttgcac
//
LOCUS       DASTKC010000018         6474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1199762, whole genome shotgun sequence.
ACCESSION   DASTKC010000018 DASTKC010000000
VERSION     DASTKC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199762"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFR25_00690"
     CDS             <1..451
                     /locus_tag="VFR25_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939595.1"
                     /translation="TWPVSLDAYLARASKEKSKAVDDGFGGTVDVTLDATTYEFGLGV
                     NKTWQRKKFYPYLNAGAVLAKIDVTARQAGTSVSDDDKGLGFWGGGGVFYRVGTTFNL
                     GGAVRYSSANVEFNAFDTGNVSFDGADVPAGGLTFGVLLGWGWPKTP"
     gene            complement(448..2205)
                     /locus_tag="VFR25_00695"
     CDS             complement(448..2205)
                     /locus_tag="VFR25_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9939596.1"
                     /translation="MVSAVQARRAAAAIALLGLMAPLAAGSCFAVTLTRYPSVWLRTK
                     GSVTIAWQTDVPSSGTVLFGPTPGLGASRSHGGTLVDHAVLVDGLDPGSTYYYRVVSG
                     ADTLGLTEQVATAPDGGAPFRFLAFGDIGAATPEQVEVASRIDSLNADLAILTGDIIY
                     EAGEAQNFTPQYFDVYRPTIARIPFYPSLGNHDVVTSNGLPYLEAFYLPVNTSTGTER
                     YYSFDYANAHFAALEVTVENVPPDPTMLAWLDQDLAATSQLWKFVYFHVPALSNAGVH
                     GGDPVIQAALDPILEARGVDVVFQGHNHFYTRTYPTIGTTPVAVVQEPDYLNPGAPIY
                     IVTGGGGRALYPLGPALPFEAFSKSTFHVTSVDVDGNTLTLRAIERDGHVMDSMTLTK
                     EVPTAVALLDYAAESDADGVRLRWRVSRSTDVSGFHVYRGPGPGTVEERLTAEPLRGE
                     AEAFEYLDRTAVVGASYYYALGAVDLRGREERVGLVRGTRGAPYRFEISGAVPNPFAR
                     GSEIRFTLDRASRVDVSIHDASGREVRRLMPGPLGSGPHALAWDGRDGRGRVAAAGVY
                     FVRIRSEQREAVAKLVRVP"
     gene            complement(2225..3007)
                     /locus_tag="VFR25_00700"
     CDS             complement(2225..3007)
                     /locus_tag="VFR25_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9939597.1"
                     /translation="MNRVFRTAVVLVTAAGLAGCVSSGKFNKLKAEQDVLKNEKAELE
                     KQKADLEKQAADLTQSNTQLKSDIDRLNTESASLAAARDSLAKTHEQAVSQYDAVVNQ
                     LSAEVEKGNLQVRQYKNMLTVDVAEQIFFDTGSFTLKKSGQEVLKKVGDAIANYPDKA
                     IRVVGHTDNVPYKSGKWTNWELSAMRATTVVRFLQDKSKIEPARLVSAGRGEYEPVAS
                     NDTPEGRQKNRRIEITLLDKSYLETMAPQQATTGAAPSGESH"
     gene            complement(3343..3945)
                     /locus_tag="VFR25_00705"
     CDS             complement(3343..3945)
                     /locus_tag="VFR25_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HET9939598.1"
                     /translation="MNQRFAVLLATAAVALAVPLAATVAEEAAKADVAAKADEAKIPA
                     GQKLFLDNGCNSCHSMKGQGIEKKKSATATEATASTPAASPSKIPDLSAVGLEHDQAW
                     MTKFLLKTETLHDKKHMKTWKGTEAELATLTTWLAEQKNAEAAGLKTADKAAKEADAA
                     VQKADEAKQEAKEAKKDADKAADQAKDAKEDAEKAKEQGK"
     gene            4187..5773
                     /locus_tag="VFR25_00710"
     CDS             4187..5773
                     /locus_tag="VFR25_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9939599.1"
                     /translation="MTATTARKSRRPGSARAADSTKARRAPLDFVRFVPLILLLVVAA
                     SVTLYLEAEHRIAGRWGFSLDDSWIYAQMARNVATGHGFAFNPGEPVAGSTGPLYTFI
                     LALFYALFHEVVWSAKIFGIACLAGSAFAIYRAVRALVPERPGAALAAGLLLATAPAL
                     LWGSVSGMEITLYLLLVSLGIDAYARGRPNLATLLWSVGVWVRPDGLFLAMLALVFGP
                     PREIWKRALCAAPPILAFLAFNHAIGGHWMPQTVGVKAHFGIVTSHTVNMIREWGALW
                     GVPYRSTDDLEEPVLLLALLAIGAVLLFRRRPILALYAIGFPIALSLFREHSASHKRY
                     ILYVIPFGITLAAVGLGGIASRLGGKRGGAALAALGGACLVWQLAIARGEAETHGWNV
                     QNINEMQRTMGEFARRVTKPGDRIAANDIGAIGYFSERPVVDLVGLITPVEPLPRMLS
                     RYKPELMIVFVDWFRSDVLWDPDSRGFVFLDADSTHKYMIIGAVELKHNTISAKDQML
                     AFRRLPMDAPAPERLLMEAR"
     gene            complement(5775..6260)
                     /locus_tag="VFR25_00715"
     CDS             complement(5775..6260)
                     /locus_tag="VFR25_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9939600.1"
                     /translation="MPADVALTKARNEVLEFLGETFERVRGIYLDRGTSFFETLADVT
                     ASEASRRASPDTASVGAHVRHCIYYLEVLQRALKGEPLGTLDWRSIWTNDRPVDEGEW
                     DAIRQQLRNEVSAVEAMAKDDATWEREDALGEFIAVITHTAYHLGAVRQALRVIRAAQ
                     S"
     gene            complement(6260..>6474)
                     /locus_tag="VFR25_00720"
     CDS             complement(6260..>6474)
                     /locus_tag="VFR25_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET9939601.1"
                     /translation="PDPKQDNPKRLVVKLKAEKALPFAVPLSAFRADDILATTELVRL
                     PRLSIVPLTKAHFTRLLERAGMKGGR"
BASE COUNT          943 a         2189 c         2167 g         1175 t
ORIGIN      
        1 gacgtggccc gtgagcctgg acgcgtatct cgcgcgcgcg tcgaaggaga agtcgaaagc
       61 cgtcgacgac ggcttcggcg gcacggtgga cgtcacgctc gacgcgacga cgtacgagtt
      121 tggcttgggg gtgaacaaga cctggcagcg gaagaagttc tatccctacc tgaacgcggg
      181 cgccgtcctc gccaagatcg acgtcacggc gcgccaggcc ggcacgtcgg tgagcgatga
      241 cgacaaggga ctcggcttct ggggcggcgg cggcgttttc taccgggtcg ggaccacgtt
      301 caacctcggc ggcgcggtcc ggtacagctc cgcgaatgtc gagttcaacg cattcgacac
      361 cggaaacgtg tccttcgacg gcgccgacgt gcccgcgggc gggctcacgt tcggcgtcct
      421 cctcggctgg ggctggccga agactcccta gggaacgcgc acgagcttcg ccacggcctc
      481 gcgctgctcg gaccgtatcc ggacgaagta gacgccggct gccgcgacgc ggccgcggcc
      541 gtccctgccg tcccacgcga gcgcgtgcgg accgctcccg agcgggccgg gcatgagacg
      601 gcggacctcg cggcccgacg cgtcgtggat cgagacatcc acccgtgacg cgcggtccag
      661 cgtgaaccgg atctcgcttc cccgagcaaa gggattgggg acagcgccgc tgatctcgaa
      721 tcgatagggc gcaccccgag ttccgcgcac caacccgacg cgctcctcgc ggcctcgaag
      781 gtccacggcg ccgagcgcgt agtagtagct ggccccgacc acggccgtgc ggtccagata
      841 ttcgaacgcc tcggcctcac cgcggagcgg ctccgccgtg aggcgctcct ccacggtccc
      901 ggggcccggc ccccggtaca cgtggaagcc ggacacgtcc gtggacctgg acacccgcca
      961 cctgaggcgc accccgtcgg cgtcggactc ggccgcgtag tcgaggagcg cgaccgcggt
     1021 cgggacctcc ttggtgagcg tcatcgaatc catcacgtgg ccatcccgct cgatggcgcg
     1081 gagcgtgagc gtgttcccgt cgacatcgac ggaggtcacg tggaaggtgc tcttcgagaa
     1141 cgcttcgaac ggcaacgccg gcccgagtgg atagagggcc cggccaccgc ctccggtgac
     1201 gatgtagatg ggcgctcccg gattcaggta gtcgggttcc tggaccacgg ccaccggcgt
     1261 cgttccgatc gtcgggtagg tacgcgtgta gaagtggttg tgcccctgga acaccacgtc
     1321 cacgccgcgc gcctcgagga tgggatcgag tgctgcctgg atcaccggat caccgccgtg
     1381 cacgccggcg ttggagaggg ccggcacgtg gaagtacacg aacttccaga gctgggacgt
     1441 cgcggcgagg tcctggtcca gccacgcgag catcgtcggg tccggcggca cgttctccac
     1501 cgtgacctcg agagccgcga agtgggcgtt cgcgtagtcg aaagagtagt agcgctcggt
     1561 gccggtcgag gtgttgaccg gcaggtagaa cgcctcgagg tacgggaggc cgttggacgt
     1621 gaccacgtcg tggtttccga gcgacgggta gaacgggatc cgcgcgatcg tcggacggta
     1681 cacgtcgaag tattgcggcg tgaagttctg cgcctcgccc gcctcgtaga tgatgtctcc
     1741 ggtcaggatc gccagatcgg cgttcaagga atcgatccgc gaggcgacct cgacctgctc
     1801 cggcgtcgcg gcgccgatgt cgccaaaggc gaggaagcga aacggcgcac cgccgtccgg
     1861 ggccgtcgcg acctgctcgg tgaggccgag cgtgtccgcg ccggacacga cacggtagta
     1921 gtacgtcgac cctgggtcga gtccgtcgac caggaccgcg tgatccacga gggttccccc
     1981 gtgggagcgc gacgcgccca gaccgggagt ggggccgaag aggaccgttc cggaggacgg
     2041 aacgtccgtc tgccatgcga tcgtgaccga acccttcgtg cgaagccata cggaggggta
     2101 gcgcgtcagc gtgaccgcaa agcaagagcc cgccgcgagt ggcgccatca ggcccagcag
     2161 ggcgatggcg gccgcggcgc ggcgggcttg cacggcgctc accatggagc gtcgtcgaag
     2221 aatgctagtg gctctctcca ctcggggccg ccccggtcgt tgcctgctgc ggcgccatcg
     2281 tctcgagata gctcttgtcg aggagcgtga tctcgatgcg ccggttcttc tgccgtccct
     2341 cgggcgtgtc gttcgaggcg accggctcgt actccccgcg ccccgcggac acgagccgcg
     2401 ccggttcgat cttgctcttg tcctggagga agcggaccac ggtcgtcgcc cgcatcgccg
     2461 agagctccca gttggtccac ttgccgctct tgtacggcac gttgtccgtg tgtccgacga
     2521 cacggatcgc cttgtccgga tagttcgcga tcgcgtcgcc gactttcttc aatacttcct
     2581 ggccggactt cttcagcgtg aagctccccg tgtcgaagaa gatctgctcg gcgacgtcca
     2641 ccgtgagcat gttcttgtac tggcgaacct ggaggttccc cttctccacc tccgccgaga
     2701 gctggttcac cacggcgtcg tactggctca cagcctgctc gtgcgtcttc gcgagcgaat
     2761 cgcgcgcggc cgcgagcgaa gcggactccg tattgagccg gtcgatgtcg gacttgagct
     2821 gggtgttgga ctgcgtgaga tcggccgcct gcttctcgag atcggccttt tgcttctcga
     2881 gctcggcctt ctcgttcttg aggacatcct gctccgcctt cagcttgttg aactttccgc
     2941 tggagacgca tccggccaga ccggccgccg tcacgagcac gacggcggtc ctgaacactc
     3001 ggttcatggg ggttcctcct tcgaggcgcg cgaggccctc gcatcggttg ggcccgcgtt
     3061 cgcgcgggct ccgtcgggag aggggcgcca ctctcccgag cgatacgtcc aggtatcggt
     3121 cgcggatccg gaggattgag agcttgtcag gatgtggtct caccggacca gccacgcgag
     3181 taaaacgcgt tgcaggattc cacggacccg ttcgcccagc cgcatcctgc acctcctccc
     3241 cccaggtgtc aaatcgccgg gcacacggcc gcgagggcaa gaaagacggc cggccccaac
     3301 ccggggaccg gccgtctgag acgcgctgac gcgcggtgtc gactacttgc cctgttcttt
     3361 cgctttctcg gcgtcttcct tggcgtcctt cgcctgatcg gccgccttgt cggcgtcctt
     3421 cttggcttcc ttcgcttcct gcttcgcctc gtccgctttc tgcacggccg cgtccgcttc
     3481 cttggccgcc ttgtccgccg tcttcagccc tgcggcttcg gcgttcttct gctccgcgag
     3541 ccaggtggtg agcgtggcga gctcggcctc ggtccccttc catgtcttca tgtgcttctt
     3601 gtcgtgaagg gtctcggtct tgaggaggaa tttcgtcatc cacgcctgat cgtgctcgag
     3661 tccgacggcc gataggtcgg ggatcttgct gggggaagcg gcgggcgtcg acgctgtggc
     3721 ctccgtcgcc gtcgcggact tcttcttctc gatcccctgt cccttcatcg agtgacagga
     3781 gttgcatcca ttgtcgagaa agagcttctg gcccgcggga atcttggctt catcggcctt
     3841 ggcggccacg tcggccttcg ccgcttcctc cgccacggtc gcggcgagcg ggacggcaag
     3901 cgccaccgcc gccgtcgcga gcagcactgc gaatcgctgg ttcacgggac tacctccttc
     3961 acgggggact gcgcctggac ccaagggaat tatcgcaaga aatagccctt ttcgggagtc
     4021 atcctgacgt ctaccagggg atgcgctccc ggtccttgtg gaaccctcct atccggccta
     4081 ggttcgacga aggtcgcgag cgggaccatg gtttccgtgg gtcgaggcta tcacgcgcgg
     4141 cagcttggag cttgatccgg ctccggagcg cgcggagaat cccgccatga ctgcgacgac
     4201 cgcgcggaag tccaggcgcc cgggatcggc acgcgccgcg gactcgacca aggcccgtcg
     4261 cgcgcccctc gatttcgtgc gcttcgttcc gctcattctc ctcctggtgg tggccgcgag
     4321 cgtgaccctc tacctcgagg cggagcatcg gatcgctgga cggtggggct tctcgctgga
     4381 cgactcctgg atctatgccc agatggcgcg gaacgtcgcc acgggacacg gattcgcgtt
     4441 caatcccggc gagcccgtcg cgggctcgac cggccccctc tacacgttca tcctcgccct
     4501 cttctacgcg cttttccacg aggtcgtctg gagcgccaag atcttcggca tcgcctgtct
     4561 cgcgggatcg gcgttcgcga tttatcgagc ggtgcgcgcg ctggtgccgg agcgacccgg
     4621 agccgcgctc gcggcgggac tccttctcgc caccgccccg gcgctcctct ggggatcggt
     4681 gtcggggatg gagatcacgc tctatctcct cctcgtgtcc ttggggatcg acgcctatgc
     4741 ccgagggcga ccgaacctcg cgacgctcct ctggagcgtc ggcgtgtggg tgcgcccgga
     4801 cggtcttttc ctcgcgatgc tcgcgctcgt gttcggtccc ccgcgcgaga tctggaagcg
     4861 cgcgctctgc gcggcgcctc cgatcctcgc gttcctcgcg ttcaatcacg cgatcggtgg
     4921 gcactggatg cctcagaccg tcggggtcaa ggcgcacttc ggcatcgtca cgagccacac
     4981 ggtcaacatg atccgcgaat ggggagcgct gtggggggtc ccctaccgaa gcacggacga
     5041 cctcgaagag ccggtgctcc tcctcgcgct cctcgcgatc ggtgcggtgc tcctcttccg
     5101 gcggcgcccc atcctcgcct tgtacgcgat cggcttcccg atcgcgctgt cgctcttccg
     5161 ggagcacagc gcgtcgcaca agcggtacat cctgtacgtc atcccgttcg gcatcacgct
     5221 ggcggcggtg ggtctcggtg ggatcgcttc gcgcctcggt gggaagcgcg gaggggcggc
     5281 gctggccgcg ctcggcggcg cctgcctcgt ctggcagctc gcgatcgcgc gcggagaggc
     5341 ggagactcac gggtggaacg tccagaacat caatgaaatg cagcgcacga tgggcgagtt
     5401 cgcgcgccgc gtcacgaagc cgggagatcg catcgcggcg aacgacatcg gggcgatcgg
     5461 gtacttcagc gagcggccgg tcgtggatct cgtggggctc atcacgccgg tcgagccgct
     5521 gcctcggatg ctgagccgct acaagcccga gctcatgatc gtattcgtgg actggttccg
     5581 ctcggacgtc ctctgggatc cggacagccg tggcttcgtg tttctcgacg ccgactccac
     5641 gcacaagtac atgatcatcg gcgccgtgga gctgaagcac aacacgatca gcgcgaagga
     5701 tcagatgctg gcgttccggc ggctccccat ggatgctccc gcccccgagc ggctcctgat
     5761 ggaggcgcgc tgagctagct ctgcgcggct cggatgaccc ggagcgcctg tctcaccgca
     5821 cccaggtggt acgccgtgtg ggtgatcacc gcgatgaact cgcccagcgc gtcctcgcgc
     5881 tcccacgtgg catcgtcctt ggccatcgct tcgaccgcgg aaacttcgtt ccggagctgc
     5941 tgccggatcg cgtcccattc gccctcgtcc acaggacgat cgttggtcca gatcgagcgc
     6001 cagtcgagcg tgccgagcgg ctcgcccttg agtgcgcgct ggagaacctc gagatagtag
     6061 atgcaatgcc ggacgtgggc gccgacgctg gccgtgtcgg gcgatgcccg cctcgacgcc
     6121 tcggacgcgg tcacgtccgc gagcgtctcg aagaacgacg tgccccggtc gaggtagatc
     6181 ccgcgcaccc gttcgaacgt ctcccccagg aattcgagca cttcgttccg cgccttcgtg
     6241 agcgcgacat cggccggcat cagcgccccc ctttcatccc ggcgcgctcg aggagccgcg
     6301 tgaagtgcgc cttggtgagg ggcacgatcg agaggcgcgg cagccgcacc agctcggtcg
     6361 tcgccaggat gtcgtcggcg cggaacgcgc tcagcggaac cgcgaacggg agcgccttct
     6421 cggccttcag cttcacgacg agccgtttgg ggttgtcctg cttgggatcg ggga
//
LOCUS       DASTKC010000019         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_491137, whole genome shotgun sequence.
ACCESSION   DASTKC010000019 DASTKC010000000
VERSION     DASTKC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491137"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFR25_00725"
     CDS             <1..746
                     /locus_tag="VFR25_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET9939602.1"
                     /translation="GCGIVAGLANVMGGMLAVGTRTWDHVRQSYFVATGAGFMLAAVI
                     LRMIPESYRLNVASTGDGRGALSVGLLILLGYLLVHFAEHVLVGHFHFGEETHHEHWV
                     EPVVGTSALVGLLLHTLLDGVSIGSAFVVQPALGILVAVAVFLHKVPEGFTIGSIMVA
                     AGRSRRDAFLSAAWLGVATVVGTLTISLWSGLVPYALPLSAGAALYVAASDLIPAVNE
                     TKGIKMALVVFGGVVLFFATESVLERLGL"
     gene            861..1448
                     /locus_tag="VFR25_00730"
     CDS             861..1448
                     /locus_tag="VFR25_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET9939603.1"
                     /translation="MGLEELVPDGILTTTVEKMVNWARRSSIWPATFGLACCAIEMMA
                     TSASRYDLARFGAEVFRASPRQADLMIVAGRVSKKMAPVLKRIYDQMPEPKWVISMGA
                     CASCGGEFKDYAIVQGVDTVVPVDVYVPGCPPRPEALIYGIMKLQEKIDRMKLKGDHP
                     APAMLVETPQPTGSTPGSGQITPMSGVLPPNPGPA"
     gene            1462..2649
                     /gene="nuoD"
                     /locus_tag="VFR25_00735"
     CDS             1462..2649
                     /gene="nuoD"
                     /locus_tag="VFR25_00735"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET9939604.1"
                     /translation="MSATTAPEFKTITINMGPQHPSTHGVLRLIIELDGETVVSLKPV
                     MGYLHTGMEKIMEAKSYTKSITVTDRMDYLSPLGNNMAYVGAVERIMGLTLPERAQTL
                     RVILLELARISSHLVWLGTHAIDLGAMSMFLYCFREREKLLDVNDMVAGSRMTPSYFR
                     VGGFFQDVPDTFVPYVRKFVDEFPSSIDEYHNLLTKNRIWMKRTVGVGTITAEQAVGL
                     SVTGPTLRGSGLNYDVRKAMPYCGYETYQFDVPLGKNGDVYDRYLCRMAEMRQSLAIV
                     RQGLDRLKPGSVRADNPYLFPARREDVKSGMEELIFHFKIMSEGFRPPIGDAYFSVES
                     PKGELGFYVASDGEPLPYRVRVRPPSFINLQALETMCVGRMVADVVACIGSIDIVLGE
                     VDR"
     gene            2651..>3587
                     /gene="nuoF"
                     /locus_tag="VFR25_00740"
     CDS             2651..>3587
                     /gene="nuoF"
                     /locus_tag="VFR25_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET9939605.1"
                     /translation="MESNGRVLTATTALPGSHTLEVYRKHGGYEALAIALAKTPDEVI
                     DVVKRSGLRGRGGAGFPTGTKWGFVPRSTDKPKYMCVNADESEPGAFKDRLLLAKDPH
                     QVIEGTLIGAFAIGARRAYIYIRGEFAHEAAIVQAAIDEARAAGLVGKNILKSGYDCE
                     VVIYLGAGAYICGEETALIESIEGQRGEPRLKPPFPAVVGLFGCPTVVNNVETLACVP
                     HIVKRGWEWFAGIGPDRNTGPKLYGLSGHINRPGVYEAPMGISLRTLIDEYGGGVPGG
                     RPVKGVIPGGASCPVLRGDEIDISMDFDTLTRAGSL"
BASE COUNT          573 a         1226 c         1188 g          600 t
ORIGIN      
        1 taggctgcgg catcgtggcc gggctcgcga acgtcatggg cggcatgctc gccgtcggca
       61 cccgcacctg ggaccacgta cgccaatcct acttcgtcgc gacgggggcg ggcttcatgc
      121 tcgccgccgt catcttgcgc atgattcccg aaagctatcg cctgaacgtg gcctcgaccg
      181 gcgacgggag gggcgcgctc tcggtcggcc tcctcatcct cctcggctac ctcctcgttc
      241 atttcgcgga acacgtcctc gtcggtcact tccacttcgg cgaggagacg caccacgagc
      301 actgggtcga gccggtcgtc ggcaccagcg cgctcgtggg tctcctcctc cacacgctcc
      361 tggacggcgt ctcgatcggc tccgcgttcg tcgtgcagcc ggcgctcggc atcctcgtcg
      421 cggtggcggt gttcctgcac aaggtccccg agggattcac gatcggctcg atcatggtcg
      481 cggccggacg atcgcgaagg gacgcgtttt tgagcgccgc ctggctgggc gtcgcgaccg
      541 tggtgggcac actcacgatc tccctctggt ccggcctcgt cccctacgcg cttccgctct
      601 cggcgggcgc cgcgctctac gtggctgcgt cggatctcat cccggccgtc aacgaaacga
      661 aggggatcaa gatggcgctc gtcgtgttcg gcggggtggt gctcttcttt gcgacggaat
      721 ccgtcctgga gcggctgggg ttgtgattga cccggcccta gcctgctggt agggtgacgt
      781 ttccggcatc caatccccct cggcgagcat tcgttccgtc gggggttaac cgcgcgcgga
      841 agcgccagta aggaacccac atgggactcg aagagctcgt accggacggc attctcacca
      901 cgaccgtcga gaagatggtc aactgggcgc gccgttcgtc catctggccg gccacgttcg
      961 gcctcgcctg ctgcgccatc gagatgatgg ccacgagcgc gtcgcgctat gacctcgccc
     1021 gattcggcgc cgaggtcttc cgcgcgtcac cgcgccaggc cgatctcatg atcgtcgcgg
     1081 gccgggtgag caagaagatg gcgccggtcc tgaagcgcat ctacgaccag atgcctgagc
     1141 cgaagtgggt catctcgatg ggcgcgtgcg cctcgtgcgg cggcgagttc aaggactacg
     1201 ccatcgtgca gggcgtcgat accgtggtgc ccgtggacgt ctacgtgccc ggctgcccgc
     1261 cgcggccgga ggcgctcatc tacggaatca tgaagctcca ggagaagatc gaccggatga
     1321 agctgaaggg ggaccatccc gcgcctgcga tgctcgtcga gacgccccag ccgaccggat
     1381 cgactcccgg atcgggacag atcacgccca tgtcgggcgt gctcccgccg aaccccgggc
     1441 cggcctagag ctcgagccgc aatgtccgcg accaccgcgc ccgaattcaa gaccatcacc
     1501 atcaacatgg ggccgcagca tccctcgacc cacggggtgc tccgcctgat catcgagctg
     1561 gacggtgaga ccgtggtctc tctcaagccc gtgatgggct atctccacac ggggatggag
     1621 aagatcatgg aggcgaagag ctacaccaag tcgatcacgg tcaccgaccg gatggactac
     1681 ctgtcgccgc tcggcaacaa catggcctac gtcggtgcgg tcgagcggat catggggctc
     1741 acgctccccg agcgcgcgca gaccctccgc gtcatcctcc tcgagctcgc gcgcatctca
     1801 tcgcacctcg tctggctcgg cacgcacgcg atcgacctgg gcgccatgag catgttcctc
     1861 tactgcttcc gcgagcggga gaagctcctc gacgtgaacg acatggtcgc cggctcgcgg
     1921 atgacgccca gctacttccg cgtggggggc ttcttccagg acgtgccgga cacgttcgtg
     1981 ccctacgttc ggaagttcgt ggacgagttc ccgtcctcga tcgacgagta ccacaacctg
     2041 ctcacgaaga accggatctg gatgaagcgc acggtcggcg tcggcacgat caccgccgag
     2101 caggcggtgg ggctcagcgt gaccggcccg acgctccggg gaagcggcct caactacgac
     2161 gtgcggaagg cgatgcccta ctgcggctac gagacctatc agttcgacgt gccgctcggg
     2221 aagaacggcg acgtctacga tcgctacctc tgccgcatgg ccgagatgcg ccagtcgctc
     2281 gccatcgtgc ggcaggggct cgaccgtctc aagccgggct ccgtgcgcgc cgacaatcca
     2341 tatctcttcc cggctcgccg cgaagacgtg aagagcggga tggaggagct catcttccac
     2401 ttcaagatca tgtccgaggg attccgcccg ccgatcgggg acgcgtactt ctcggtcgaa
     2461 tcgccgaagg gagagctcgg cttctacgtg gcgagcgacg gcgagccgct cccgtaccgc
     2521 gtgcgcgtgc ggcctccgtc gttcatcaac ctccaggcgc tcgagaccat gtgcgtcggc
     2581 cggatggtgg ccgacgtggt cgcgtgcatc gggagcatcg acatcgtcct cggggaggtg
     2641 gaccgctagc atggaatcga acggacgcgt cctcaccgcc acgacggccc tccccggctc
     2701 gcacaccctc gaggtctatc gcaagcacgg cggctacgaa gcgctggcga tcgccctcgc
     2761 gaagactccc gacgaggtca tcgacgtggt gaagcgctcc ggccttcgcg gccggggcgg
     2821 cgccggtttc ccgacgggca ccaagtgggg cttcgtcccc aggtcgaccg acaagcccaa
     2881 gtacatgtgc gtgaacgcgg acgagagcga gcccggcgcg ttcaaggacc ggctcctcct
     2941 cgccaaggat ccgcaccagg tgatcgaggg gacgctgatc ggcgccttcg cgatcggcgc
     3001 gcgtcgcgcc tacatctaca tccggggcga gttcgcgcac gaggcggcga tcgtgcaggc
     3061 ggcgatcgac gaggcgcgcg cggcggggct cgtggggaag aacatcctca agtccggcta
     3121 cgactgcgag gtggtgatct acctcggcgc cggggcctac atctgcggcg aggagaccgc
     3181 gctcatcgag tcgatcgagg ggcagcgcgg cgagccgagg ctcaagccgc cgttcccggc
     3241 cgtcgtcggc ctcttcggct gcccgacggt ggtgaacaac gtggagacgc tcgcctgcgt
     3301 gccgcacatc gtgaagcgcg ggtgggagtg gttcgcaggc atcggccccg accggaacac
     3361 ggggcccaag ctctacggct tgagcggcca catcaatcgc ccgggcgtct acgaagcgcc
     3421 catggggatc tcgctccgca cgctcatcga cgagtacggc gggggcgtcc ctggcggacg
     3481 tccggtgaag ggggtcattc ccggcggcgc gtcctgtccc gtgctccgcg gcgacgagat
     3541 cgacatctcc atggacttcg acacgctcac ccgcgcgggc tcgctct
//
LOCUS       DASTKC010000020         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_254842, whole genome shotgun sequence.
ACCESSION   DASTKC010000020 DASTKC010000000
VERSION     DASTKC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254842"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /gene="recJ"
                     /locus_tag="VFR25_00745"
     CDS             complement(<1..1337)
                     /gene="recJ"
                     /locus_tag="VFR25_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9939606.1"
                     /translation="MRASTPKTWVLRNPAAPEHARRLAAELGLSLTFATLLANRGFGT
                     SEEVNEFLVPSLTRLVDAFEMRDMDKATARIWEAVDKQEPILVYGDYDVDGITATTLL
                     TSALARAGAKVGYFIPDRIRDGYGFSVRGVDVARRRRTRLVITADCGITATNEVRLAK
                     EQGIDVIVTDHHEPLGELPDAVAVLNPKRKDCPYPFKELAGVGVVLKLVQGLVASRPA
                     ALPESFVMEHLDLVALGTIADVVPLRGENRIFAKIGLERICNSQKPGIVALKEVAGLR
                     ARRVESGHVAYILAPRINAAGRLGNAESGVRLLLATDTGEATTIAESLEEDNTNRKKI
                     DEETLEDALEQLRRIGPELPPAIVLWSEEWHPGVLGIVASRLTERFHRPTVLISADED
                     EGKGSGRSIPGFDVCQALQECREHLIGFGGHSYAAGLTIRAEQMQAFRVRLCDV"
     gene            complement(1431..2186)
                     /locus_tag="VFR25_00750"
     CDS             complement(1431..2186)
                     /locus_tag="VFR25_00750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9939607.1"
                     /translation="MNDLNQGAPGGVSGRAALVVGGGRGLGRAVAIALAEGGARVLAV
                     GRTPKPLDELADLARSRRLPLRTARGDAGRTATVRAWLRGVGKERAPDILVIAAGDYW
                     EGPFSKLTVERWEALLHSNTTVPLAVLQEALPLMRRRRYGRILLFGVAGGETPRAAAR
                     AHAYRAAKVALLTLAKSVAQDEAPYGITLNVILPGIIRPAFSGREADLIRRIPAGRLG
                     SPREVARAALFLLAEESGYITGAALPVAGGYLL"
     gene            2251..2589
                     /locus_tag="VFR25_00755"
     CDS             2251..2589
                     /locus_tag="VFR25_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939608.1"
                     /translation="MNDQRWEEVLRRLDKAFGDLEFDEIEDEETQAVTETVVWTGPQG
                     KMKLARTTRPLVVDKKTHFSNRIGGGTHVEYIYSKTESTSRVRLYRWNEGLNDWDEVD
                     AAELGVAAGK"
     gene            2597..>3137
                     /gene="glpX"
                     /locus_tag="VFR25_00760"
     CDS             2597..>3137
                     /gene="glpX"
                     /locus_tag="VFR25_00760"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET9939609.1"
                     /translation="MDRHVGLDLVRITESAALACSRWMGRGDKNAADQAAVTAMRRSF
                     DNVDIRGTVVIGEGERDEAPMLYIGEQVGSGWGPEVDIALDPLECTNSVAYGRPNAMA
                     VIALAARGHFLHAPDTYMEKIAVGPKAASAIDLSRPIEENLEAVAAAKGYHLDDLTVV
                     ILDRPRHEDLIQKVRKTGAR"
BASE COUNT          520 a         1059 c         1095 g          463 t
ORIGIN      
        1 acgtcgcaaa gccggacgcg aaaggcctgc atttgctccg cgcggatcgt gagcccggcg
       61 gcgtagctgt ggccgccgaa gccgatcagg tgctcgcgac attcttgaag cgcctggcac
      121 acgtcgaatc cggggatgct ccgccccgag cccttcccct cgtcctcgtc ggccgagatg
      181 agcacggtcg ggcgatggaa tcgctcggtg agccgggacg ccacgatgcc gagcacgccg
      241 gggtgccact cctccgacca gagcacgatc gcggggggca gctcgggacc aatacggcgc
      301 aactgctcga gcgcgtcctc gagcgtctcc tcgtcgatct tcttgcggtt ggtgttgtcc
      361 tcctcgaggc tctccgcgat ggtcgtcgcc tcaccggtgt cggtcgcgag gaggaggcgc
      421 acgccgcttt cggcgttccc gaggcggccc gccgcgttga tccgcggcgc gaggatgtag
      481 gccacgtgtc cgctctcgac gcgccgcgcg cgaagtcccg cgacctcctt gagcgccacg
      541 atgccgggct tctgcgagtt gcagatccgc tcgagtccga tcttggcgaa gatgcggttc
      601 tcgcctcgga gcgggacgac gtccgcgatc gtgccgaggg cgacgagatc gagatgctcc
      661 atgacgaagc tctcgggaag cgcggccgga cgcgacgcaa cgagtccctg cacgagcttg
      721 agcacaacgc ccacgcccgc gagctccttg aatggatacg ggcagtcctt ccgcttcgga
      781 ttgagcaccg cgaccgcatc gggcagctcg cccaggggct cgtgatgatc ggtcacgatc
      841 acgtcgatcc cctgctcctt cgccagccgg acctcgttgg tggcggtgat gccgcagtcg
      901 gccgtgatca cgagccgcgt ccggcgccgg cgcgccacat ccacgccccg gaccgagaag
      961 ccgtagccgt cccggatccg atcgggaatg aagtacccga ccttggcacc ggctcgcgcg
     1021 agcgcggagg tgaggagcgt cgtcgcggta atgccgtcca cgtcgtagtc gccatagacg
     1081 aggatcggct cctgcttatc gaccgcctcc cagatccggg ccgtggcctt gtccatgtcc
     1141 cgcatctcga aggcgtccac gaggcgcgtc agggacggga cgaggaactc gttgacctct
     1201 tcggaggtgc cgaagccacg gttggcaagg agggtggcga aggtcagaga aagacccagc
     1261 tccgcagcca ggcgccgcgc gtgttccggg gcagccgggt tgcgaagcac ccaggtcttc
     1321 ggggtggatg cgcgcaatca aacctttcgt gagttagagg gctagttcac ggaattgtaa
     1381 cagcttgcgg tgagagggtc aacgagaggc ggagtggggt ccgggccggt ctagagcaga
     1441 tatcccccgg caaccggaag cgccgccccg gtgatgtagc cggactcctc ggcgaggagg
     1501 aaaagcgccg cccgggccac ctcgcgggga ctcccgagcc gtcccgcggg aatgcggcgg
     1561 atcaggtcgg cctcacgccc cgagaaggcg gggcggatga ttcccgggag gatcacgttc
     1621 agcgtgatgc catacggcgc ctcgtcctgc gcgacgctct tggcgagggt caagagggcc
     1681 accttggcgg cacggtaggc gtgtgcgcgt gcggcagcgc gcggggtctc tccgcccgcg
     1741 acgccgaaga gaaggatgcg gccgtagcgg cgtcggcgca tgagcgggag cgcctcctgg
     1801 agcacggcga gcgggaccgt cgtgttcgag tggaggagcg cttcccagcg ctcgacggtg
     1861 agcttggaga agggtccttc ccagtaatcc cccgccgcga tcacgaggat gtccggcgcg
     1921 cgctccttcc ccactccgcg cagccacgcc cgcacggtgg ccgtgcgccc tgcgtccccg
     1981 cgcgcggtcc ggagcgggag gcgccgcgat cgcgcgaggt ccgcgagctc gtcgagcggc
     2041 ttgggcgtgc ggccgaccgc caggacgcgg gcaccccctt cggccagcgc gatcgcgacg
     2101 gcgcgaccga gtccccggcc acctcccacg acaagcgcgg cgcgtccgct cacgccgccc
     2161 ggggctcctt ggttcaagtc gttcatgggg tgtgctacta taggacgctt cgatgccaag
     2221 tcaatcgagc ccccacgagg gcccaattcc atgaacgatc aacgatggga agaggtgctc
     2281 cggcgcctcg acaaggcctt cggcgatctc gagttcgacg agatcgagga cgaggagacc
     2341 caggcggtca ccgagacggt ggtctggacg gggccgcagg ggaagatgaa gctcgcccgc
     2401 accacgcggc ccctcgtcgt ggacaagaag acccactttt ccaaccggat cggcggcggc
     2461 acgcacgtgg aatacatcta ctcgaagacc gagagcacga gcagggtgcg cctctaccgc
     2521 tggaacgaag gcttgaacga ctgggacgag gtcgacgcgg ccgagctggg cgtggccgcg
     2581 ggaaagtagc cgcacgatgg atcgacacgt cggcctggac ctcgtccgca tcaccgaatc
     2641 cgccgccctc gcctgctccc gctggatggg gcgcggcgac aagaacgccg cggaccaggc
     2701 tgccgtcacc gcgatgcggc gctcgttcga caacgtcgac atccgaggca cggtggtgat
     2761 cggcgaaggc gagcgggacg aggcgccgat gctctacatc ggcgagcagg tcggctccgg
     2821 ctggggtccc gaggtcgaca tcgcgctcga tcccctcgag tgcacgaatt ccgtggccta
     2881 cggccgtccg aacgcgatgg cggtcatcgc gctcgccgcg cgcggccact tccttcacgc
     2941 ccccgacacg tacatggaga agatcgccgt cggccccaag gccgcgagcg cgatcgatct
     3001 ctcgcgtccg atcgaggaga acctcgaggc cgtggccgcc gcgaagggct accacctcga
     3061 cgatctcacg gtcgtcattc tcgaccgacc gcggcacgag gacctgatcc agaaggtccg
     3121 gaagaccggg gcgcgca
//
LOCUS       DASTKC010000021        10203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_255177, whole genome shotgun sequence.
ACCESSION   DASTKC010000021 DASTKC010000000
VERSION     DASTKC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255177"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFR25_00765"
     CDS             complement(<1..683)
                     /locus_tag="VFR25_00765"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET9939610.1"
                     /translation="MFLVLAVLVIAASTARAQTWSPVGPPGGTVRDLAHDPRNPDRVY
                     LGTADGILYRSDDGGVNWSRLDPGFPTRGMSLDDIAVDTRSTVYVGYREVSGSGGGVA
                     RSTDGGHTFTLLPGIQGESVRAIALAPSSRRVIAAGTLTGVFLSRDAGQTWTRITPKD
                     HPDLRNIESLAFDPDDPRVLYAGTWHLAWRTRDGGANWTPIHQGMIDDSDVMTMTVLQ
                     DDPQVLYATA"
     gene            complement(916..2145)
                     /locus_tag="VFR25_00770"
     CDS             complement(916..2145)
                     /locus_tag="VFR25_00770"
                     /inference="COORDINATES: protein motif:HMM:NF017546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9939611.1"
                     /translation="MRSMIVAFAAVALVAAAPAFAGGDKGQIEIGGYGGWFFPDDYGQ
                     FHPDKDNIWGGRLGYWFTQNWTLEGSYQKVMTDTEMNLIGVPEVDVDLASYRLNLLYN
                     LGSAASKARLFVTAGAGSEKTDIDTYVDENKFGWNVGGGLRFFLSRNFNLRFDGRFAS
                     VKAVEGSGHEGNGEATVGLGFLFGGHKEEAAAEVIPPPANQPPTVSCAADRGQILPGE
                     TATITATAADPEGGPLTYDWTATGGARVTGTGPSATLDFSGATPPATAAISVKVTDDH
                     GQSASSDCTVALMAPAKPPEAVSCISTGFPKNASRLNNVDKACLDDVDQRLKADPRAR
                     VVVIGHSDTKETTKNIAQARAEAVRDYLVRERGIDTARIEVRAATATAGADVTAQTSN
                     RRVEVWFVPEGASVPGM"
     gene            2526..4955
                     /locus_tag="VFR25_00775"
     CDS             2526..4955
                     /locus_tag="VFR25_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9939612.1"
                     /translation="MPLSKGTKLGTYHITGPLGAGGMGEVYRARDTRLGREVAIKVLP
                     EAVASSPDRLARFEREARAVAGLNHPNIVTLHSVEEEEGVRFLTMELVDGQSLDHVVT
                     SGGLPLSRVLELAIPLANALVAAHERGVVHRDLKPGNVMVTHDGWVKVLDFGLAKVAA
                     EEGADPGVTIGATAGPISGAGQVLGTVPYMAPEQIRGETVDARSDLFALGIILYELAT
                     GRRPFTGESSADISSAILRDVPAPLLSVRSDLPRDLNRIVSRCLEKNPRDRFQTARDV
                     YNELKYLEREGSAPPSPVSGGLTPSPPSAAPTPFPHRPAPPTSGPRPATPAPSGPGSA
                     VSGFGLPSTNVPSIAVLPFVNRSRNEEDEYFSDGLADELLSVLTKVRGLRVAARASSF
                     QFRGMNEDLAVIGERLNVVTLLDGSVRKSGNRVRISVQLVQAADRVQLWAETYDRSLD
                     DIFAVQDDIAQSVVRELRTTLLGEAPDSKASSAAQAAIAAAGRGHGVQNPEAHRAYLQ
                     GKYFVDRVTEEDTAKGLRYLEEAVAIDPTLAVAYAAISRAYSNAGSYGWEPVRAAYEK
                     ARKAGLRALELAPDLPEAHIRLAAIQRVHDWDWKGAEASVRRALELDPSNAETLRAAG
                     SIAHGSGRDEEARAYFLRSIELDPLVSAGYTGLAQVYRAMDRLPDSEQALRKAMELSP
                     QRLGASMMLTILLSDQGKHAEAVREAGREGAEWARLTALAYAYQKAGQTVECDKVLRQ
                     LEEGHAVDSPYQIAAVHNAKGDVDAAFAWLNRAVEERDAGVAQVAVEPVFRNLHSDPR
                     WKPLLKRINID"
     gene            complement(4942..7665)
                     /locus_tag="VFR25_00780"
     CDS             complement(4942..7665)
                     /locus_tag="VFR25_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9939613.1"
                     /translation="MVVESASPPPSSELSDTRIVGRPVWARYGVALGTVLLAWYARQA
                     LTHAVGPTALPFIFFFPAVAVAAWWGGFGPGLMAVALSSLLANWSFMRPTWTLTLSSY
                     DLAALLAFGASCMFILGAIEMMHRARASLHGEVELRRRAEAEMSQALERSAATLASIG
                     DGVIVTDAEGRIVSINHEAERLTEWIRAEAVGRPLAEVFRIVREQDRKPADDPVARAL
                     REGRIVNLANHTLLLSRSGREIPIEDSAAPIRRAGESVQGAVLVFRDATLERRAHEAS
                     VRLAAIVESSGDAILTKTLDGTIRTWNRGAERLFGYKPEEIIGRPITTLIPPERLHEE
                     AEIIERLRSGQPYERLETVRLAKGGRRIIVSLSISPLRDADGEIVGASKIVHDVTEIV
                     AARDALAREKGLLATTLSSIGDGVIVTDAEGRVTFLNPEAERLTGWTGNDAHGLTLPE
                     VFRIVAEKTRTTVKNPVEEVLRRNAVVGLANHTILIARDGAERAIDDSAAPIRVEGVL
                     SGVVLVFRDVSERRRAEAILREEDRRKDEFLAILSHELRNPIAPIGMAVDLMRKKGST
                     DPELRELRDIIERQTNQLARLLDDLLDVSRIVSGKIVLRHEQASLGLAISSAVEAVRP
                     ALGARGHHLTVELPPDPVYVEGDLARLAQVFANLLHNAVKYTDRGGRISIGLERKGSD
                     ALVRVRDTGIGLTVDQMSRIFQMFAQGDRLTEGTQQGLGVGLALSKKLVELHRGGIEV
                     KSGGVGRGSEFLVRLPMSLRVQTHAASESSAPQPTAAPQGIRILIADDNEDSVTTLSW
                     SLSHSGHEVRTALDGLAAVEVATEFRPQLALLDIGMPRLNGYDAARQIREALGDQVTL
                     VAITGWGQQEDKRRAREAGFDRHVTKPIDIETLDRLVAEVGHPNPGAANRC"
     gene            complement(7766..8713)
                     /locus_tag="VFR25_00785"
     CDS             complement(7766..8713)
                     /locus_tag="VFR25_00785"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET9939614.1"
                     /translation="MRRTFRHRAVVAAATMCLLAAAPASGASSSLAVRDGDSWRTFWR
                     SDEAPSRFTAANPALMRAIQWKRLAPGLEWAELRLGCAAPVWRARLIVARLDPREVRL
                     TLAMDLTRDDMRPAWTIDRAPEDAILAVNAAQFTSAMPWGWVVIDGRQRLAPGAGPLA
                     SAVGIDQDGGVHWAHGDTLAAAGIVTGFQSYPTLLSHDGTVPAPLRTAGSLVSLTHRD
                     TRLAFGETRDGHILIAMTRYDAVGTMGEGIPLGPTTPEMAAIMGALGAHDAVMLDGGI
                     SAQLLLRGVDAQEPLRWHAIRKVPLALIARPRDLGRAAK"
     gene            complement(8719..9717)
                     /locus_tag="VFR25_00790"
     CDS             complement(8719..9717)
                     /locus_tag="VFR25_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939615.1"
                     /translation="MPRYASLAILSALVLGLAASAPSPARAGIGNPIKKAKDAVTKEA
                     KKETKSEPEAKPCPAPVFDDVTVELTEARLSRMVDTFHKMESILAPRMGLVEKRNKLM
                     DERGAIWDKNSEKIQNLEQKRDDVNGCYHEGYRRASDKKLQEYSQRAMSDPKLLEKYK
                     NLAMQNNAAAAAGDTAAQNKINAGIMEEMLPSREDSAQVRKECGPIPPTTKEEDQIHA
                     LDKQIQGLEDEIRKIDDKSAETQAKGLGMTPVQMGTAFERIRGFRGTSKDAESAKGER
                     KKKGESSSGSDAKSDSKSESGSNESGSAAVPHACGYTDEEAKALSKHATELVKYLN"
     gene            complement(9808..>10203)
                     /locus_tag="VFR25_00795"
     CDS             complement(9808..>10203)
                     /locus_tag="VFR25_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET9939616.1"
                     /translation="VAALTPNEHPDTTIILSTRGNQRRAVREGWFDFKVKGAPARLEA
                     VRLLEPGVGEQDYSLFFTDATTGKESYKVGRYLEPVKRSDGLYTLDFNMCYNPACAYS
                     EHYNCPIPPKENHLQVAIAAGEMDGHYLH"
BASE COUNT         1655 a         3402 c         3378 g         1768 t
ORIGIN      
        1 gccgtggcgt agagcacctg cggatcgtcc tggagcacgg tcatcgtcat gacgtccgag
       61 tcgtcgatca tgccttggtg gatcggggtc cagttggcgc ctccgtcacg cgtcctccac
      121 gccaggtgcc acgttcccgc gtaaaggacc cgcgggtcgt ccggatcaaa ggcgagcgac
      181 tcgatgttgc ggagatcggg gtgatccttg ggcgtgatgc gggtccaggt ctgacccgcg
      241 tcgcgcgaga ggaacactcc ggtgagcgtg cccgctgcga tcacgcgacg gctcgacgga
      301 gccagtgcga tcgcccgaac cgattcgccc tggatcccgg gcagcaacgt gaacgtatgc
      361 cccccgtccg tgctccgcgc gacgccgcct ccgctccccg acacttcccg ataaccgacg
      421 tacaccgtgc tgcgcgtgtc gacggcgatg tcgtcgaggc tcataccccg cgtcgggaat
      481 ccggggtcga gccgggacca gttcacgccg ccgtcgtcgg accggtagag gatgccgtcc
      541 gcggttccga gatagacgcg gtcgggattc cgaggatcgt gcgcgagatc gcgaacggtg
      601 ccgcctggag ggcccaccgg ggaccacgtc tgagcccgcg cggtcgacgc ggcaatcaca
      661 agaacagcca gcacgaggaa cacagaacgt gctccggcca gcccctttcg acaaacaggc
      721 atcagccctc cgatcgaggt aactcgtccg ttcttcaccc gttcggctct ctgttccgta
      781 atggcgcgca tggcgatttc cttgacagtg catagggtgt gcctcggtgg cacacgggac
      841 gcgtaagtca aacgaagcgg ggcgattccg gtgggaatcg ccccgcaatc aaccgatgtc
      901 gtttgttcgt ccgacttaca ttcctggcac cgacgcgcct tcgggaacga accaaacctc
      961 gacgcgacgg ttgctcgtct gggccgtaac gtcagcgccg gcagtcgccg tcgcggcgcg
     1021 aacctcgatg cgcgccgtgt cgatgccacg ctcgcgcaca aggtaatcgc gcacggcttc
     1081 ggcgcgagct tgggcgatgt tcttcgtcgt ctccttcgtg tcggagtgcc cgatcacgac
     1141 gacgcgcgcg cgtggatcag ccttgagcct ctggtccacg tcatccagac aggccttgtc
     1201 gacgttgttg agccgcgacg cattcttcgg gaatccggtg gagatgcagg acacggcctc
     1261 gggaggcttc gccggagcca tgagggccac cgtgcagtcg gacgaagcgc tctggccgtg
     1321 atcgtccgtc accttcaccg agattgcggc cgtcgcggga ggcgttgcgc ccgagaagtc
     1381 gagcgtcgcc gaggggccgg tccccgtcac gcgcgcacca cccgtcgcgg tccagtcgta
     1441 cgtcaacgga ccaccctcgg gatctgccgc ggtggcggtg atggtcgcgg tctcacccgg
     1501 gaggatctgc ccgcgatcgg ccgcgcacga taccgtggga ggctgattcg cgggaggcgg
     1561 aatcacctcg gccgcggctt cctccttgtg accaccgaag aggaatccaa ggcccaccgt
     1621 ggcctcgccg ttcccttcat gcccgctgcc ctcgaccgcc ttgacactgg cgaaacgtcc
     1681 atcgaagcgg agattgaaat ttcgcgacag gaagaagcgg agcccaccgc cgacgttcca
     1741 tccaaacttg ttctcatcga cgtacgtatc gatgtcggtc ttttcggagc cggcgcctgc
     1801 ggtgacgaag agacgagcct tggaggcggc ggagccgagg ttgtagagga ggttgaggcg
     1861 ataggacgcc aggtcgacat ccacctcggg cacaccgatc agattcattt ccgtgtccgt
     1921 catgaccttc tgatatgaac cctcgagcgt ccagttctgc gtgaaccagt atccgaggcg
     1981 tcccccccag atgttgtcct tgtcgggatg gaactgcccg tagtcgtccg ggaagaacca
     2041 tccgccgtaa ccaccgattt cgatctgtcc tttgtcgccg cccgcaaagg cgggggccgc
     2101 agccacgagc gccacggcgg cgaatgcaac gatcatgctt cgcatgggcc accctttctt
     2161 cttcgtcggt tgacgccggg ccatatcgcg cggcgacccg aggcaaagct cggatccaca
     2221 aggggcgatc caaagtggtc gccgatccag cccgacaaca aacttgccgc gctccaatgc
     2281 cccccacagt gggggccggt gtgccacatc gcagaagtga tgccaaccgc gtagtttgac
     2341 tcgataggag cgagtacaga gttgcgacgc agagacttac gttgcggacg tagttcggcg
     2401 atgacgggat gggtgtaggg acgagaaggc ggccctggga aaagggttcc acgggtcggt
     2461 atgttccttc aaagctcggg cgccgccgct gacagggagt cgccgtacgg ttacactacg
     2521 cggctatgcc cctctccaaa ggaaccaagc tcggcaccta ccacatcacc ggcccgctcg
     2581 gagcgggagg tatgggcgag gtctatcgcg cgcgcgacac gcgcctgggg cgcgaggtcg
     2641 cgatcaaggt gctgcccgag gccgtggcgt ccagtcccga tcggctcgcg cgcttcgagc
     2701 gagaagcgcg cgccgtcgcc gggctcaacc atccgaacat cgtcacgctc cactcggtcg
     2761 aggaggagga aggcgtccgc ttcctgacga tggagctggt cgatggacag agcctcgatc
     2821 atgtggtcac gtcgggaggg cttcccctct cgcgcgtgct cgagctcgcg atcccgctcg
     2881 ccaacgccct cgtcgccgcc cacgaacgcg gcgtcgtgca tcgcgatctc aagcccggga
     2941 acgtcatggt gacgcacgac ggctgggtga aggtgctcga cttcgggctc gcgaaggtcg
     3001 ccgcggagga aggcgccgat cctggcgtga ccatcggcgc gaccgcgggg ccaatctcgg
     3061 gtgccgggca ggtcctgggg accgtgccgt acatggcgcc ggagcagatc cgcggcgaga
     3121 ccgtcgacgc gcgctccgat ctcttcgcgc tcggcatcat tctctacgag ctcgctacgg
     3181 gccgccggcc cttcacggga gagagctcgg ccgacatcag ctcggccatc ctacgtgacg
     3241 tgccggctcc ccttttgagc gtgcgttcgg acctcccgcg cgatctgaac cggatcgtca
     3301 gccgctgtct cgagaagaat ccgcgcgacc gcttccaaac ggcgcgcgac gtgtacaacg
     3361 agctgaagta tctcgagcgc gaggggagcg cgccgccgtc gcccgtatcg ggcggcttaa
     3421 cgccctcgcc gccctccgcc gcgccgacgc cgttcccgca ccggccggca cctccaacat
     3481 cgggacccag accggcgacc cctgctccgt ccgggcccgg ctcggcagtc tccggctttg
     3541 gacttccatc cacaaacgtg ccctcgatcg ctgtgctccc cttcgtcaac cgtagccgga
     3601 acgaagagga tgaatacttc tccgacgggc tcgccgatga gctcctgagc gtgctgacga
     3661 aggtgcgtgg cctacgcgtc gcggcgcgcg cctcgtcgtt tcagttccgt ggcatgaacg
     3721 aggatctcgc cgtgatcggc gagcggctga atgtggtgac gctcctcgat ggaagcgtgc
     3781 ggaaatcggg caatcgggtg cggatctcgg tgcagctcgt tcaggcggcc gaccgcgtgc
     3841 agctctgggc cgagacctac gaccgctcgc tcgacgacat cttcgcggtc caggacgaca
     3901 tcgctcaatc cgtcgtccgg gagcttcgaa ccacactgct cggggaagct ccggactcga
     3961 aggcgagcag cgccgctcag gccgccatcg ccgcggccgg ccggggccac ggagtccaga
     4021 atcccgaggc tcaccgcgcc tacctgcagg gcaagtactt cgtcgatcgc gtgacggagg
     4081 aagacactgc gaagggactc cgctatctcg aggaggcggt cgcgatcgac ccgaccctcg
     4141 cggtcgccta cgccgccata tcccgggcgt actcgaatgc gggcagctat ggttgggagc
     4201 ccgtgagggc ggcctatgag aaggcgagga aggcggggtt gcgcgcgctc gagcttgcac
     4261 cggatctgcc cgaggcgcac atcaggctcg ccgccatcca acgagtgcac gattgggatt
     4321 ggaagggggc ggaggcgtcc gtgcggcgcg cgctcgagct cgacccgtcc aatgccgaga
     4381 cgcttcgcgc tgcgggcagc atcgcacacg gctcagggcg agacgaggaa gcgagagcgt
     4441 acttcctacg atccatcgag ctggatccgc tcgtatcggc cggatacacc gggctcgcgc
     4501 aggtgtatcg agccatggac cgcctgcctg attccgagca ggcactgcgc aaggccatgg
     4561 agctttcgcc ccagcgcctc ggcgcgtcca tgatgctgac catccttctc agcgaccagg
     4621 ggaagcacgc ggaagcggtg agagaggcgg gccgcgaggg cgccgagtgg gcgcgactca
     4681 cggccctcgc gtacgcgtat cagaaagcag gacagaccgt ggagtgcgac aaggtactgc
     4741 gccagctcga ggaggggcat gccgttgact ccccgtacca gatcgcggcc gtccacaatg
     4801 ccaaaggtga cgtggatgcc gccttcgcgt ggctcaaccg ggccgtcgag gagcgggatg
     4861 cgggtgtggc tcaggtcgcg gtggaaccgg tcttccggaa tctccactcg gatccgcgct
     4921 ggaagccact gctgaagagg atcaacatcg attagctgct ccggggttcg gatggcccac
     4981 ttccgccacc agtcgatcca gggtctcgat gtcgatcggc ttcgtgacgt gacggtcgaa
     5041 gcccgcctcg cgggcgcgcc gcttgtcttc ctgctggccc catccggtga tcgcaacgag
     5101 cgtgacctga tcccccagcg cctccctgat ctggcgcgcg gcatcgtagc cgttcaatcg
     5161 gggcatcccg atgtcgagga gcgcgagctg cgggcggaac tccgtggcga cctcgactgc
     5221 ggccaggccg tccaacgcgg tgcgtacttc atgacctgaa tgagacaagg accagctcaa
     5281 ggtcgtgacg gagtcctcgt tgtcgtccgc gatcaggatc cggatgccct gcggagctgc
     5341 cgtgggttgc ggggctgagc tctcggaggc ggcgtgcgtc tgcaccctca ggctcatggg
     5401 cagccggacc aggaattcgc tcccgcgccc gacgccaccg ctcttcactt cgatcccgcc
     5461 gcgatgcagc tcgaccagct ttttcgagag agccaacccc acgccaagac cctgctgggt
     5521 accctcggtc aggcgatccc cctgggcgaa catctgaaag atgcgggaca tctgatccac
     5581 ggtgaggccg atcccggtgt cgcgaacgcg cacgagtgcg tcgctcccct tccgctcgag
     5641 accgatcgag atcctgccgc cacggtcggt gtacttcacc gcgttgtgaa ggaggtttgc
     5701 aaagacctga gcgaggcgtg ccaggtcccc ctcgacgtac acggggtcgg gcggaagctc
     5761 cacggtcaag tggtgtcccc tggccccgag cgcaggtcgc acggcctcga cggcgctcga
     5821 gatcgcgaga cccaggctgg cctgttcgtg acgaagcacg atctttccgg atacgatccg
     5881 gctcacgtcc agaaggtcat ccaggagacg ggcgagctgg ttcgtctgcc gctcgatgat
     5941 gtccctgagc tctcggagct ccgggtccgt cgatcccttc ttgcgcatga ggtcgacggc
     6001 catcccgatc ggggcgatcg gattccggag ctcgtgggac aggatcgcga ggaactcgtc
     6061 cttgcgccgg tcttcctcgc gaaggatcgc ctccgcgcgc cggcgctccg agacatcgcg
     6121 gaacacgagc accaccccgg ataggactcc ttccacgcgg atcggagccg cactgtcgtc
     6181 gatcgcgcgc tccgctccgt cgcgagcgat caggatcgtg tggttcgcga gcccgacgac
     6241 cgcattgcga cggagcacct cctccaccgg atttttcacg gtggtccgcg tcttctcagc
     6301 cacgattcgg aacacctcgg gcagcgtcaa gccgtgggca tcgttcccgg tccacccggt
     6361 caagcgctcg gcttccgggt tgaggaacgt cacacggccc tcagcgtcgg tcacgatcac
     6421 accgtctccg atgctcgaca gcgtcgtcgc gaggagcccc ttctcacgcg ccagcgcgtc
     6481 gcgcgcggct acgatctccg tgacgtcgtg gacgatcttc gacgcgccga cgatctcgcc
     6541 gtcggcgtcc cggagcggcg agatgctcaa cgacacgatg atgcggcggc cgcccttggc
     6601 cagccgcacg gtctcgagac gctcgtacgg ctgtccactc ctgagccgct cgatgatctc
     6661 ggcctcctcg tggaggcgct ccggcggaat gagggtcgtg atcggccggc caatgatctc
     6721 ctcgggcttg tagccgaaca gccgctcggc gccacgattc cacgtgcgaa tagtgccatc
     6781 gagcgtcttg gtcagaatcg catcccccga ggactcgacg atcgccgcga gccgcacgct
     6841 cgcctcgtgt gcgcgacgct ccagggtcgc atcccggaac acgagcacgg cgccttggac
     6901 ggactcgccc gcacgccgga tcggcgcggc gctgtcctcg atggggatct cgcgcccgct
     6961 tcgggacagg agaagcgtgt gattggcgag attgacgatc cggccctcgc gaagcgcccg
     7021 cgccaccggg tcgtcggcag gctttcgatc ctgctcgcga acgatccgga acacctcggc
     7081 gagcggccgc ccgaccgcct ccgcccttat ccattccgtg agccgttccg cctcatggtt
     7141 gatcgacacg atgcggcctt ccgcgtcggt cacgatgacg ccgtcgccaa tgctggccag
     7201 ggtcgcggcc gaccgttcga gcgcctgcga catctccgct tcggcgcgac ggcgcagctc
     7261 cacctcgccg tggaggctgg cccgcgcccg gtgcatcatc tcgatcgccc cgaggatgaa
     7321 catgcaggag gcgccgaacg cgagcaatgc cgcgagatcg taggaggaga gcgtcagcgt
     7381 ccacgtcgga cgcatgaacg accaattggc caggagcgag gaaagtgcga ccgccatgag
     7441 gccggggccg aatccgcccc accaggcagc aaccgcaacg gcaggaaaga agaagatgaa
     7501 cgggagcgcc gtaggaccga ccgcgtgggt cagcgcttgg cgcgcgtacc aggcaaggag
     7561 gacggtccca agggcgacgc catagcgcgc ccagaccggc cgcccgacga tgcgcgtgtc
     7621 gctgagctcg ctcgacggtg gaggcgacgc gctctcgacg accataggtc ggctcttatc
     7681 gattatgggt gcacttacgt aggaccttgg ggggagggcc ttttgtctat tgcaatgacg
     7741 ttagcacgat tggacgagag gaaagctatt tcgccgcgcg tcccaggtct cgagggcgag
     7801 cgatcagggc gagcggaacc ttgcggatgg catgccagcg gagcggttcc tgcgcgtcga
     7861 cgccccgcaa gagaagctgt gcggagattc caccgtcgag catgacggca tcatgagcgc
     7921 cgagcgctcc catgatggcg gccatctccg gggtcgtggg tccgagcgga attccctccc
     7981 ccatcgtccc caccgcgtcg tagcgcgtca tcgcgatcag gatgtggcca tcacgcgttt
     8041 cgccgaacgc gaggcgcgtg tcgcgatgcg tgagactgac cagactgcct gctgttcgca
     8101 gcggagccgg aacggtccca tcgtgggaga gtagcgtcgg ataggactgg aatccggtga
     8161 cgatgcccgc cgcagcgagc gtgtcgccgt gcgcccagtg aacgccgccg tcctggtcga
     8221 tgcccaccgc gctggccagc ggtcccgctc ccggagccag ccgctggcgg ccgtcgatca
     8281 cgacccatcc ccaaggcatg gcgctcgtga actgcgccgc gttcacggcg aggatcgcat
     8341 cctccggcgc tcggtcgatg gtccacgcgg gacgcatgtc gtcccgcgtc aggtccatcg
     8401 cgagcgtgag gcgaacttcc cgcggatcga ggcgcgccac gatgagccgc gcgcgccaca
     8461 ccggggcggc acagccgagc cggagctcgg cccactcgag cccgggggcg agccgcttcc
     8521 actggatggc ccgcatgaga gctggattcg cggccgtgaa tcgggacgga gcttcgtccg
     8581 agcgccagaa cgtgcgccag gaatcgccgt cgcggactgc gaggctcgag gaggcccctg
     8641 aggcgggggc ggccgccagg aggcacatcg tggcggccgc gaccacggcg cgatgtcgga
     8701 acgtgcggcg catcgggtct agttcagata cttgacgagc tccgtggcgt gcttcgagag
     8761 cgccttcgcc tcttcatcgg tgtagccgca cgcgtgggga accgctgcgg agcccgattc
     8821 attggagcct gattcggact tggagtcgga cttggcgtcg gagcccgagc tcgattcacc
     8881 cttcttcttc cgctctccct tggcgctctc cgcatccttc gaggttcccc tgaatccccg
     8941 gatccgctcg aacgccgtac ccatctgcac cggggtcatg cccaggccct tcgcctgggt
     9001 ctcggcggac ttgtcgtcga tctttcggat ctcgtcctcc agcccctgga tctgtttgtc
     9061 gagcgcgtgg atctgatcct cttccttcgt ggtgggcgga atcggtccgc actccttgcg
     9121 aacctgagcg gagtcctcgc ggctggggag catctcctcc atgattcctg cgttgatctt
     9181 gttctgtgcg gcggtgtcgc cggcagcggc cgctgcgttg ttctgcatgg cgagattctt
     9241 gtacttctcg aggagcttgg ggtcgctcat cgcccgctgc gagtactcct gcagcttctt
     9301 atccgaggct cgccggtagc cttcgtggta gcagccgttg acgtcgtctc gtttctgctc
     9361 gaggttctgg atcttttcgc tgttcttgtc ccagatggcg ccccgctcgt ccatgagctt
     9421 gttccgcttc tccacgagcc ccatgcgcgg cgccaggatc gattccatct tgtggaaggt
     9481 gtcgaccatg cgactgagac gcgcctcggt cagctcgact gtcacgtcgt cgaagacggg
     9541 cgccgggcaa ggcttcgcct cgggctccga cttggtctcc ttcttcgcct ccttggtgac
     9601 ggcatccttg gccttcttga ttggattgcc gatcccggcg cgggctggcg agggcgcgct
     9661 cgcggcgagc cccaggacga gcgcggacag gatcgcgagg gaggcgtagc gtggcatgcg
     9721 agttcctccg gtcagggtgc ggcccggctc gggccggacg ggatggacct tcctggtcga
     9781 gaacgcgcga aaacggaccg cttcggctca gtgcaggtag tgcccgtcca tctcccccgc
     9841 agcgatggcc acctggaggt ggttctcctt gggcgggatg gggcagttgt agtgctccga
     9901 gtaggcgcag gcagggttgt agcacatgtt gaaatcgagg gtgtacaaac cgtcggagcg
     9961 cttcaccggc tcgagatacc ggccgacctt gtagctctcc ttcccggtcg tcgcgtcggt
    10021 gaagaagagg ctgtagtcct gctcccccac ccccggctcg agcagccgaa ccgcctcgag
    10081 acgggccggg gctcccttca ccttgaagtc gaaccacccc tcgcgcaccg cgcgccgctg
    10141 gttgccgcgc gtggaaagga tgatcgtggt gtcggggtgt tcgttgggcg tgagcgcggc
    10201 gac
//
LOCUS       DASTKC010000022         5481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1674627, whole genome shotgun sequence.
ACCESSION   DASTKC010000022 DASTKC010000000
VERSION     DASTKC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674627"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFR25_00800"
     CDS             complement(<1..241)
                     /locus_tag="VFR25_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939617.1"
                     /translation="MTVQEAARVAEHYRALREDAMRIDAPWLSAVRLVGPDTRAFLQG
                     LATQDLERTEPGRGAITVFTTERGRPIALAWALVEA"
     gene            350..691
                     /locus_tag="VFR25_00805"
     CDS             350..691
                     /locus_tag="VFR25_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9939618.1"
                     /translation="MTTTKRIPLIPARGRGIGKDDEELAHLAKALGHPARVAIVRLLL
                     ARGECVCGGIVDRLPLAQATVSQHLKVLKEAGLVEGEIDGPRVCYCAAPEAVERLSHL
                     IQALIETKGRT"
     gene            691..1461
                     /gene="arsM"
                     /locus_tag="VFR25_00810"
     CDS             691..1461
                     /gene="arsM"
                     /locus_tag="VFR25_00810"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET9939619.1"
                     /translation="MDIRDAVRERYGSIALNDGGCCGSGCGCGDGDASSALGYTEDQK
                     QAIPQGSNLNLGCGNPLGHAAVRPGETVLDLGSGAGIDVFLASREVGPTGRAIGVDMT
                     PAMISRARKNAAEGDYANVEFRLGEIENLPVADQSVDLIISNCVINLSPAKPRVFAEA
                     MRALRPGGRMVVSDLVLTRSLPDEVRRSVEAYVGCVAGAMREEEFLAAMRNAGFDEVE
                     ILEQRGYATGGTAAQPDVSADAWSAVRSIKVRGMKPRQ"
     gene            complement(1484..2059)
                     /locus_tag="VFR25_00815"
     CDS             complement(1484..2059)
                     /locus_tag="VFR25_00815"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008730059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9939620.1"
                     /translation="MSWTPPTNADAPLTDWIRPVPDWPQPGVLFRDLTPLWADGRAWA
                     RTVGALARPFDAAPPDLVLGIEARGFLVAAALAARWGTGLVLARKPGKLPAAAIRQDY
                     ELEYGRATLETHRDLIARGARVLIADDVIATGGTAAASRAMVEALGCSVAGFAFLVEI
                     EPLGGREKLGSGAPVHSVVVYDGSGGASVHE"
     gene            2102..3001
                     /locus_tag="VFR25_00820"
     CDS             2102..3001
                     /locus_tag="VFR25_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET9939621.1"
                     /translation="MRIAIGGASGLIGTALTRHLTSQGHQVLRLVRRPARDATEVGWN
                     PGAAIIDAAALEGVDAAVNLSGSTIARWPWTGGHRRRVLESRTRSTALLARTLAALTP
                     LPSVFVSLSAVGYYGDRGSEILTETSGAGSGFLAEVCRAWESAADPAAAAGIRVATPR
                     VGVVLSGDGGMLRVLATPFRLGLGGPVGSGRQYISWISIDDLVQVIALLIERDDRSGP
                     WNAVAPQPVPQAEFARTLGHVLHRPAILPFPAFAARFLLGGLADLLLEGQRVEPARLL
                     SSGFTFRHPTLETALEAALHRKR"
     gene            3017..4375
                     /locus_tag="VFR25_00825"
     CDS             3017..4375
                     /locus_tag="VFR25_00825"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9939622.1"
                     /translation="MAKKRLIVLGGGFAGLDVVRSVARSKAARETYDTLLIDKENFFQ
                     FNPLLPAIAVGSVETRHIVYPLREMARHRHIRFQKNKAMTIDLERREVRLHNDLVEPY
                     DTLVISMGSVTNYYGVPGAPENTRPFKTIVDAMTLRARFVELFEMAEQAETREQRRRL
                     LSFAMVGGGVTGVEVAAELAEMARETLLPRYPSLAADDITITIYEGGDRVVPTARPEH
                     SAYIHRYLTKHGVRIELGAKVERVEPKCLHFAGGTTAPAFTIIWTAGVHPPELVRSLP
                     LTHIADGRVRVDPYLRALDPAGWPVEDLYVLGDCAASPTEGGRWQPALSQTGIAMGSY
                     VGECLVRRAEGKPLRTFQFKDAGYIISLGKHSSVLDLFGIPLSGKLAWLLWAGAYLVK
                     MVGFRKQVEVAFDQLTHLVFEHDTSQILNRRQVLTDEELNLSLGGEEPSSERIGTRRQ
                     AS"
     gene            4531..4914
                     /locus_tag="VFR25_00830"
     CDS             4531..4914
                     /locus_tag="VFR25_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939623.1"
                     /translation="MRRVVGALMMTALLAMNGAAVAHDGEHAAGKAMTAVGEIVDTGC
                     YLSHGASGAKHAQCAQLCISKGMPMGLLTATGDLYLLTPPHDTMTAYKKAKDWAGKKV
                     EISGPMSERNGMKSIEVASAKLAPA"
     gene            4929..>5481
                     /locus_tag="VFR25_00835"
     CDS             4929..>5481
                     /locus_tag="VFR25_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9939624.1"
                     /translation="MKLRVIALALVGALILGIGALAFLTRAQLTPVERGRRLAEATGC
                     FACHGAEGIRGMANPGRDEKQVPSFEGTLMMYAKNTEEVREWIRDGGTKARREDPDWQ
                     AERKLGVVRMPAFGKRLSAAQIEDLVAYVVAVGGQNAPDDSLASHGKDRADELGCVGC
                     HGPGGQLARANPGSLKGYIPPWDG"
BASE COUNT          878 a         1862 c         1895 g          846 t
ORIGIN      
        1 ccgcctcgac cagcgcccac gcgagagcga tgggccgccc ccgctcggtc gtgaacacgg
       61 tgatcgcccc ccgtcccggc tccgtgcgct cgagatcttg cgtcgcgagt ccctggagga
      121 acgcgcgcgt gtcggggcct acgaggcgta cggcggaaag ccacggcgcg tcgatccgca
      181 tcgcatcctc gcggagcgcg cggtagtgct ccgccactcg agctgcttcc tggaccgtca
      241 tgcctggaga atagcatcgg gcctctgtgg aaccggtcgc gggccgaaat gggccttgac
      301 ggcctgacgc ttttatcgta tatttacgat taacgatgaa aggaggcgaa tgacgaccac
      361 caagcgcata ccgctgatcc cggcgcgcgg ccgcggcatc ggcaaggacg acgaggagct
      421 ggcccatctg gccaaggccc tggggcatcc cgcccgggtc gccatcgtcc ggctcctgct
      481 cgcacgcggc gaatgcgtgt gcggcggaat cgtggaccgc ctgcctctgg cccaggcgac
      541 ggtgtcccaa catctcaagg ttctgaagga agccggcctc gtcgagggag agatcgacgg
      601 accccgcgtc tgctactgcg ccgctccgga agcggtggag cggctgtcgc acttgatcca
      661 agccctgatc gaaacgaaag gaaggacgtg atggacattc gtgatgcggt tcgcgagcgg
      721 tacggctcga ttgccttgaa cgacggaggc tgctgcggca gtgggtgcgg gtgcggcgat
      781 ggcgacgcct cgtccgctct cggctacacg gaggaccaga aacaggcgat cccccaagga
      841 tcgaacctga atctgggctg cggcaatccg ctcggccacg cggcggtccg gccaggcgag
      901 accgtcctcg atcttggctc gggcgcaggc atcgacgtgt tcctcgcgtc gcgcgaggtg
      961 ggacccaccg ggcgcgccat cggcgtcgac atgacgccgg cgatgatttc gagagcgcgg
     1021 aagaacgcag ccgagggaga ctatgcgaac gtcgagtttc ggctcggcga gatcgagaat
     1081 ctcccggtcg cggatcaaag cgtcgatctc atcatctcca actgcgtgat caatctctcg
     1141 cccgccaagc ctcgcgtctt cgccgaggcg atgcgggcgc tccgtccagg cggccgcatg
     1201 gtggtgagcg atctcgtgct cacgcgttcg ctgcccgatg aggtgcgccg atcggtcgag
     1261 gcgtacgtcg gctgtgtcgc gggagcgatg cgggaggagg agtttctcgc cgcgatgcgg
     1321 aacgctggct tcgacgaggt cgaaatcctg gagcagcggg ggtatgcgac cgggggcact
     1381 gcggctcagc ccgacgtatc cgcggacgcg tggagcgcgg tccggtcgat caaggtgcga
     1441 ggcatgaagc cgcggcagtg acaacacggg cgcccgcgcc ggactattcg tgaaccgagg
     1501 cgcccccgga tccgtcgtag acgaccacgc tgtggacggg ggcgccggag cccagtttct
     1561 cgcgtccgcc caggggctcg atctccacga ggaacgcgaa gccggccacg ctgcacccga
     1621 gcgcctcgac catcgctctc gaggccgcgg ccgtgcctcc ggtcgcgatc acgtcgtccg
     1681 cgatcaggac gcgcgcgccg cgtgcgatca gatcgcgatg cgtctcgagc gtcgcgcgcc
     1741 catactccag ctcgtagtcc tgtcggatcg ccgccgccgg cagcttcccc ggcttccgcg
     1801 cgagcacgag gccggtgccc caccgcgccg cgagcgcggc cgccacgagg aatccgcgcg
     1861 cctcgatgcc cagaacgaga tccggcgggg ccgcgtcgaa cggacgcgcc agcgcgccga
     1921 ccgtgcgcgc ccaggcgcga ccgtccgccc agagcggcgt gagatcgcga aagagaacgc
     1981 cgggctgagg ccagtccggc accggccgga tccagtcggt gagcggcgcg tcggcgttgg
     2041 tgggcggagt ccaagacacg cgcggaccct agcaggcgcg accctccgct acaatcgagg
     2101 catgaggatc gcgatcggcg gcgcttccgg gctcatcggc acggcgctga cgcgccacct
     2161 cacctctcag gggcaccaag tgctccggct cgtgcgccgc cccgctcgcg acgccaccga
     2221 ggtcggctgg aatccaggcg ccgccatcat cgacgccgcc gcgctcgagg gcgtcgacgc
     2281 cgcggtcaac ctttccggga gcaccatcgc gcgctggccc tggaccgggg ggcatcgccg
     2341 tcgcgtgctc gagagccgca cgcgctccac cgcgctcctc gcgcgaaccc tggccgcgct
     2401 cacgccgctc ccgagcgtgt tcgtttcctt gtcggcggtc ggctattacg gcgatcgcgg
     2461 cagcgagatc ctcaccgaga cttcgggcgc aggcagtggc ttcctcgccg aggtctgccg
     2521 cgcgtgggaa tccgccgcgg atcctgccgc ggccgcggga atccgcgtcg ccaccccgcg
     2581 ggtcggggtg gtcttgagcg gagacggcgg catgcttcgg gtcctcgcga cgccgtttcg
     2641 cttgggactc ggcggaccgg tgggctcggg acgtcaatac atcagctgga tctcgatcga
     2701 cgacctcgtc caggtgatcg cgctcctgat cgagcgggac gatcggagcg gcccctggaa
     2761 tgcggtcgcg ccccagcccg ttccgcaggc cgagttcgcg cggactctcg gccacgtcct
     2821 gcatcgcccc gcaattctcc ccttccccgc gttcgcggcg aggttcctgc tcggcggcct
     2881 ggccgacctg ctgctcgagg gccagcgcgt cgagccggcg cgccttctct cgtccggctt
     2941 caccttccgc caccccaccc tcgagacggc gctcgaggct gcgctccacc gcaagaggta
     3001 ggtgtatcct cgaatcatgg cgaagaagcg gctcatcgtt cttggaggag ggttcgcggg
     3061 gctcgacgtg gtccgctccg tggcgcggtc gaaggcggcg cgcgagacct acgacacgct
     3121 cctcatcgac aaggagaact tcttccagtt caatccgctc ctgccggcga tcgccgtggg
     3181 ctccgtcgag acgcgccaca tcgtctaccc gcttcgcgag atggcgcgcc accgccacat
     3241 ccgcttccag aagaacaagg ccatgaccat cgacctcgag cggcgcgagg tgcgcctcca
     3301 caacgatctg gtcgagccgt acgacacgct cgtcatctcc atgggcagcg tgaccaacta
     3361 ttacggcgtg ccgggcgcgc cggagaacac gcgccccttc aagacgatcg tcgacgcgat
     3421 gacgctccgc gcgcgcttcg tcgagctgtt cgagatggcg gagcaggcgg agacgcgcga
     3481 gcagcgccgc cgtcttctct ccttcgccat ggtcggaggc ggcgtcacgg gggtcgaggt
     3541 cgcggcggag ctcgccgaga tggcccgcga gacgctcctc ccgcgctatc cgagcctcgc
     3601 cgcggacgac atcacgatca caatctacga aggcggcgat cgcgtcgtgc cgaccgcgcg
     3661 gccggagcac tccgcgtaca tccaccgcta cctgacgaag catggcgtgc ggatcgagct
     3721 cggagcgaag gtggagcgcg tggagccgaa gtgccttcac ttcgcgggcg gaaccaccgc
     3781 tcccgcgttc acgatcatct ggaccgccgg cgtgcatccg ccggagctgg tgcgctcgct
     3841 gccgctcacg cacatcgcgg acggccgcgt gcgcgtcgat ccctatctcc gcgcgctcga
     3901 tcccgcgggg tggcccgtcg aggacctcta cgtgctcggc gactgcgcgg cgtcgccgac
     3961 cgagggcggg cggtggcagc cggcgctctc ccagaccggc atcgccatgg ggagctacgt
     4021 cggggagtgc ctcgtgcggc gggccgaggg aaagccactc cgcacgttcc agttcaagga
     4081 cgccggctac atcatctcgc tcggcaagca cagctccgtc ttggacctct tcgggattcc
     4141 cctgtccgga aagctggcct ggctcctctg ggccggcgcc tacctcgtga agatggtcgg
     4201 gttccggaag caggtggagg tggcgttcga ccagctgacc catctggtct tcgagcacga
     4261 cacgtcccag atcctgaacc gccgccaggt cttgaccgat gaggagctga atctgtccct
     4321 gggcggcgaa gaaccctctt cggagcgaat cggaacccgc cgccaggcat cgtgacgtag
     4381 tgagggtccg tctgtaacag aacgtgggtc ggctggactc aaccatcgtt cgagttgcgc
     4441 cggagggacc cgacgagtac gttcaggggt aacttcaggg ggaagccggt tttacggggc
     4501 cttcgcccct cacaggtcag gaggacgacg atgcgacgag ttgtgggtgc cctcatgatg
     4561 acggcgctgc tcgcgatgaa cggagcggcg gtcgcgcatg acggggagca cgcggccggc
     4621 aaggcgatga cggcggtcgg cgagatcgtg gatacgggct gttatctgag ccatggcgcc
     4681 tcgggcgcca agcatgccca gtgcgcccag ctctgcatat cgaagggaat gcccatgggc
     4741 ctcctcaccg ccacgggcga tctctacctg cttaccccgc cgcacgacac gatgaccgcc
     4801 tacaagaaag cgaaggactg ggcgggcaag aaagtggaga tctcgggccc gatgagcgag
     4861 cgcaacggca tgaagtcgat cgaggttgcg tccgcgaagc tggccccggc gtaacaaccg
     4921 catcgacgat gaagcttcgc gtcatcgcac tggccttggt gggagccctg atcctcggga
     4981 tcggggctct cgcttttttg acgcgcgcgc agctcacacc cgtcgagcgg ggccgtcgcc
     5041 tcgctgaagc gacgggatgc tttgcgtgtc acggcgccga ggggattcgc ggcatggcga
     5101 atccaggacg cgacgagaaa caggtcccct cgttcgaggg cacgctcatg atgtacgcga
     5161 agaacaccga ggaagtgcgc gagtggatcc gagacggcgg gacgaaggct cggcgcgagg
     5221 accccgactg gcaagcggag cgcaagctgg gcgtggtgcg catgcccgcg ttcggcaagc
     5281 gcctcagtgc cgcccagatc gaggacctcg tcgcgtacgt tgtggccgtg ggaggccaga
     5341 acgctccgga cgactccctc gcatcgcacg gtaaggatcg cgccgacgag ctggggtgcg
     5401 tgggatgcca cggtccgggt ggacagcttg cgcgcgcgaa tcccggatcg ctcaagggct
     5461 acattccgcc ctgggacggg g
//
LOCUS       DASTKC010000023         6297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1202233, whole genome shotgun sequence.
ACCESSION   DASTKC010000023 DASTKC010000000
VERSION     DASTKC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202233"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFR25_00840"
     CDS             <1..214
                     /locus_tag="VFR25_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET9939625.1"
                     /translation="LTAAHRTLPFGSRVRVTNLENGLSVVVEVNDRGPFRKGRVIDVS
                     EEAARELGFYRAGTARVRVEELASRE"
     gene            complement(306..962)
                     /locus_tag="VFR25_00845"
     CDS             complement(306..962)
                     /locus_tag="VFR25_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939626.1"
                     /translation="MKGKQAGLVAVVAVAFLTMGCGKQEHQKLASDTSKLEADVLQQP
                     VATTASMVQTTPRSEQNEGLPMAADSLPPDVVASASALVADRGQVVEITAQGSTDITG
                     MIMSDGLNKTPLTYDMDARAWRGVYRVPLRTKADRVGLAVTATNGRGQWQRVWVFIEV
                     GRPEVGGEEKTSVEEKTGVEQQTGTETQGITEPAQGTGEQGTEPAKEAEPTQTENGNG
                     "
     gene            1266..2351
                     /locus_tag="VFR25_00850"
     CDS             1266..2351
                     /locus_tag="VFR25_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939627.1"
                     /translation="METLTRLRRALLGAGLIVAAVLLRAVAIAAPFDARVIWTHGDRA
                     YLVARDSVWVTAGSSLRFFDKKKEVASSVVVAIEDVALVVVRITSGSLSRVKHLDRLR
                     VDAERPVARTALRVGIPSSRRIQPFFECQPRLSPRGFSADFFDGPFQGFSPHGMNLPD
                     SLYLREFDDAADEEIALERGEIDAAIFWPGEASTHIREAMTWTGTPRLVRSTGVIGVR
                     AKKPWSLRELSTGRLPKGAEEKLARLNADVFRGDLVPIHYANAESLAAWSFEVDPRLP
                     GRELIQRALDPSAHLPSGTGTALLTYFDEPTHPGFGSDALPEPELWLFLIGCPVLSRP
                     ELRPYLESIDLSAIVNGFHCDIRVQKP"
     gene            2348..3970
                     /locus_tag="VFR25_00855"
     CDS             2348..3970
                     /locus_tag="VFR25_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9939628.1"
                     /translation="MRSLRTRLILGIALVAIVPLGLALVLMSKRVETMVREESGRRLE
                     TGVRALQAEMRERLHGTEAKLGILSRDPLLRRLYLVRAGSARELGDYLAERRILLDLD
                     YLEVDGLDSIPVATATGSPADSTLAVKLDLTSPITYDNHWVGTLRGGVLLDRDFLIRL
                     QQRRGLDLVLFDRSGRLAASTLRDTLEHTLPPEASRATIGDGTYWGLSAPLELGASGT
                     VTLTALTPTTPSDETIAALRLTAVLLALGGIVVAMLLGFVWSRQVSRPVERLAAHSRR
                     LARGEWDEPISLHSVRELETLGEALDRMRADLVAYREKLVLSERHAAWSQMARKVAHE
                     VKNPLTPIAISVADLKRSYDLQRPDFPTVLDQAVRTIAAEVDSLKRLLQEFSDFARFP
                     APTLAPVQAGALLDDLRALYAREIEEGRLAVSTAPPITLRADAGQLRQALVNLVKNGI
                     EAVNGSGKVTVEARPAGRSAEFTVTDNGPGLTAEQQAGLFTPGFTTKSHGSGLGLTIV
                     DRIVSEHGGTIAVQSDEGRGTTFVIRLPREEA"
     gene            3974..5314
                     /locus_tag="VFR25_00860"
     CDS             3974..5314
                     /locus_tag="VFR25_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9939629.1"
                     /translation="MPSILIVDDEANIRSSLEGALSREGYDTETAASVAEARTKLKDA
                     YDVVLLDVWFPRESGLDLLGEIAESAPETVVIMMSGHASIDTAVQATRLGAFDFLEKP
                     ISLERLLVLLRNATDSLSLRSENRRLRQAWADSIVGDSPAIRALIKEIQLAASSHARV
                     LIQGENGTGKELVARALHAAGPRRDKPFVAVNCAAIPEELIESELFGHERGAFTGATQ
                     SRRGRFEEAHGGTLFLDEVGDLSQRAQTKLLRVLQEGEFFRVGGNRAIKVDVRVIAAT
                     NRDLAELTRAESFREDLYFRLAVVPLTVPPLRERLEDIPVLVTHLAADVLRGTGGRGK
                     SFSPSAIERLARYPYPGNVRELRNVVERLIIMTPSTRIGADAVDRVLGSAVEPHGADH
                     DDSRLAEQVRRFEKDRIEETLRLEGGNVTKAAARLGLERSHLYKKMRKLGIESA"
     gene            complement(5748..6083)
                     /locus_tag="VFR25_00865"
     CDS             complement(5748..6083)
                     /locus_tag="VFR25_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939630.1"
                     /translation="MSGAAGFGATGEGAGVGIGIAGETGAADGENAGVVTGSSPLDAA
                     LLDERECLAGAAADACCGAFAAAAGFVAVAAAGAVDGAGVAAAPVWIGCGVGTGDGTE
                     SVAAAAGAG"
BASE COUNT         1018 a         2219 c         2022 g         1038 t
ORIGIN      
        1 cctcaccgcc gcgcaccgca cgctcccctt cggcagccgt gtgcgcgtga ccaatctcga
       61 aaacggtctg agcgtcgtgg ttgaggtgaa cgaccgggga ccattccgga aggggcgcgt
      121 catcgacgtg tcggaggagg ccgctcgcga gctgggcttc tatcgcgcgg gaaccgcgcg
      181 cgtgcgcgtg gaggagctgg ccagccgcga gtagtgagct cgttcgtgga aatgaggagc
      241 cccgcggact ttcggtccgc ggggctttgt cgtctcgccg cgctcgccgc gcggtcatcg
      301 tctcatcagc cgttgccgtt ctccgtctga gtcggctcgg cctcctttgc gggctccgtg
      361 ccctgctcgc cagtaccctg cgccggctcg gtgatgccct gcgtttcggt accggtctgc
      421 tgctcgacgc ccgtcttttc ctcgacgctc gtcttctcct caccgcccac ctccggcctc
      481 cccacctcga tgaagaccca cacgcgctgc cactggcccc ggccgttcgt cgccgtgacc
      541 gcgagtccga cgcggtcggc cttggtgcgc agcgggacgc gatagacgcc gcgccacgcc
      601 ctggcgtcca tgtcatacgt gagcggcgtc ttgttgaggc cgtccgacat gatcatcccc
      661 gtgatgtccg tcgagccctg cgccgtgatc tccacgacct ggccccgatc cgcaacgagc
      721 gcggaggcgc tcgcgaccac atccggaggc agcgaatccg ccgccatggg gagtccttcg
      781 ttctgctcgg aacggggcgt cgtctgtacc atgctcgccg tcgttgccac cggttgctgc
      841 agcacatccg cttccagctt gctcgtgtcg ctcgccagct tctgatgctc ctgcttgccg
      901 caacccatcg tgagaaacgc caccgccacc accgccacga gacccgcctg cttgcccttc
      961 atgtccttct cccgtccgga tcaatccgcc ggtgtcgggc aggtcgccgg agcctgaccc
     1021 gagccgcctg gtggtcggca catgccccgt gcaccaggac cgggagggcg ggacttgttg
     1081 ccgccgctcg ggactggcct agatcaagca atgggccaaa tggggatgcg attggaatca
     1141 aatgacttag ctgtttgaca agcgcgaggg aggctgcgaa acgtgtctag attgacacat
     1201 agcctgcgtc ggagcaggct cttagaggac tcaatccaat gcatctcaag ccgataagag
     1261 aaactgtgga aactctgaca cgccttcgcc gcgcactcct cggggccggg ctcatcgtgg
     1321 cggccgtgct cctgcgtgcg gtcgcgatag cagctccgtt cgacgcacgt gtgatctgga
     1381 cgcacggaga ccgcgcctac ctcgtcgcgc gcgattccgt gtgggtcacg gcaggctcga
     1441 gcctgcggtt cttcgacaag aagaaggaag tcgcctcgag cgttgttgtt gcgatcgagg
     1501 acgtggcgct cgttgtggtg aggatcacat cgggctcact ctcgcgcgtg aagcacctcg
     1561 accgacttcg cgtggacgcg gagcggccgg tggcgagaac cgcgcttcga gttgggattc
     1621 cgtcgtccag acgcatccag ccattcttcg aatgccaacc caggctgagc ccgcgcggct
     1681 tcagtgcaga cttcttcgat ggcccgttcc agggattcag tccccatggc atgaacctcc
     1741 ctgactcgct ctatcttcgc gagttcgacg acgcggccga tgaggagatt gcgctggaac
     1801 gcggcgagat cgacgctgcg atcttctggc ctggggaggc gtcgactcac attcgcgaag
     1861 caatgacatg gacgggaacg cccaggttgg tccggtccac aggggtcatc ggcgttcgag
     1921 ccaagaaacc atggtccttg cgtgaactct cgaccgggcg tcttcccaaa ggggcggagg
     1981 aaaagctcgc ccgcctcaac gccgatgtct ttcggggaga tctcgtcccg atccattacg
     2041 cgaatgccga gtccctggca gcatggtcct tcgaggtcga ccctaggctt cccgggcgtg
     2101 agctgattca gcgggcactc gacccctcgg cacatctccc tagcggaact ggcactgctc
     2161 ttctcaccta tttcgacgag ccaactcacc cagggttcgg cagcgatgcc ctgcccgagc
     2221 ctgagctatg gctgttcctc atcgggtgcc cggtcctgag ccggcccgag ctccgcccct
     2281 atctggagtc cattgatctc tcagcgatcg tgaacggctt ccattgcgat atcagggtgc
     2341 agaagccatg agatccctgc gcacgcgact catcctggga atcgccctcg tcgcgatcgt
     2401 gccgcttggg ctcgcgctcg tgctgatgtc caagcgcgtg gagacgatgg ttcgcgagga
     2461 atcaggacga cgtctcgaga ccggcgtccg cgcgcttcag gcggaaatga gggagcgcct
     2521 ccacgggacc gaggccaagc tcgggatcct gagccgcgat cccctcctcc gacggctcta
     2581 cctcgttcgg gcgggtagtg cacgcgagct gggcgactat ctcgcggagc gccgcatcct
     2641 tctggatctg gactatctcg aggtcgacgg gctcgacagc atccccgtgg ccaccgcgac
     2701 cggatcccca gccgattcga cgctcgcggt gaagctcgat ctgacgagtc ccatcaccta
     2761 cgacaaccac tgggtgggca cgcttcgcgg cggcgttctc ctggatcgcg atttcctgat
     2821 ccgcctccag cagcgccgcg ggctcgatct cgtcctcttc gatcgttctg gccggctggc
     2881 cgcctcgacg ctccgcgaca ctttggagca cacactccca cccgaggctt cgcgcgccac
     2941 gatcggagac ggaacctact ggggcctgag cgctccactc gagctcggcg catccggcac
     3001 cgtgacgctc accgcgctca cgcccacgac tccgtccgac gagacgatcg ccgcgctccg
     3061 cctcacggct gtgctcctcg cgctcggcgg catcgtggtc gcgatgctgc tcgggttcgt
     3121 ctggtcccgc caggtctccc gcccggtcga gcgcctggcg gcgcactcgc gccgcctcgc
     3181 gcgcggcgag tgggacgagc cgatctcgct ccacagcgtg cgcgagctgg agaccttggg
     3241 cgaggcgctc gaccgcatgc gcgcggatct cgtggcctac cgggagaagc tcgtcctgag
     3301 cgagcgccac gccgcgtgga gccagatggc gcgcaaggtg gcccatgagg tgaagaatcc
     3361 gctcacgccg atcgcgatct ccgtcgcgga cctgaagcgg agctacgacc tccagcggcc
     3421 cgatttcccc accgtcctcg atcaggccgt gcggacgatc gccgcagagg tcgattcgct
     3481 gaagcggctg cttcaagaat tctccgactt cgcgcgcttc cccgccccca cgctcgcgcc
     3541 cgtgcaggcc ggtgcgctcc tcgacgacct acgggcgctc tacgcgcggg agatcgaaga
     3601 gggccgtctc gccgtctcaa ccgctccccc gatcacgctg cgcgccgacg ccggacagct
     3661 ccggcaggcg ctcgtcaatc tcgtgaagaa cggcatcgag gcggtaaacg gcagcggcaa
     3721 ggtcacggtg gaagcgaggc cggcgggtcg gagcgccgaa ttcacggtca ccgataacgg
     3781 tcccgggctc accgcggagc agcaggccgg gctcttcacg ccgggattca cgacgaagtc
     3841 gcacgggagc ggcctcggtc tcaccatcgt cgaccgcatc gtttccgagc atggcggcac
     3901 catcgcggtc cagtcggacg agggacgcgg caccacgttc gtcattcgcc tgccgcgcga
     3961 ggaggcctga gccatgccgt cgatcctgat cgtcgacgac gaagccaaca tccgctcgag
     4021 cctcgaaggc gcgctctcgc gcgagggcta cgacacggag acggccgcga gcgtcgccga
     4081 ggcgcggacg aagctcaagg acgcgtacga cgtggtcctc ctcgacgtct ggttcccccg
     4141 cgagagcggg ctcgacctcc tgggcgagat cgcggaatcc gcgccggaaa cggtcgtgat
     4201 catgatgtcc ggccacgcgt cgatcgacac cgcggtccag gcgacgcggc tcggcgcgtt
     4261 cgactttctc gagaagccga tctcgctcga gcgcctgctc gtgctccttc ggaacgccac
     4321 cgactccctg tcgctgcgct cggagaatcg ccgtttgcgg caggcgtggg cggactccat
     4381 cgtcggcgac tcccccgcga tccgggccct catcaaggaa atccagctcg ccgcatcgag
     4441 ccatgcgcgc gtgctgatcc agggagagaa cggaaccgga aaggagctgg tcgcccgcgc
     4501 gctccacgcc gccggaccgc gtcgcgacaa gccgttcgta gcggtgaact gcgcggccat
     4561 ccccgaggag ctgatcgagt ccgagctgtt cgggcacgag cggggcgcgt tcacaggcgc
     4621 cacgcagtca cggcgagggc ggttcgaaga ggcgcacggg ggcactctct tcctggacga
     4681 agtcggagac ctgagccagc gggcgcagac aaagcttctc cgcgtgctcc aggagggtga
     4741 gttcttccgc gtcggcggca atcgcgcgat caaggtcgac gtgcgcgtga tcgcggccac
     4801 gaaccgggac ctcgccgagc tcacgcgcgc ggagtccttc cgcgaagacc tctacttccg
     4861 cctcgccgtc gtgccgctca cggtaccgcc gctccgcgag cggctggagg acatccccgt
     4921 gctcgtcacg cacctcgcgg ccgacgtgct ccgtgggaca ggcggccgag gaaagagctt
     4981 cagcccttcg gcgatcgagc gcctggcgcg ctatccctat ccaggaaacg tgcgcgagct
     5041 caggaacgtg gtcgagcggc tgatcatcat gacgcccagc acgcgcatcg gagcggacgc
     5101 ggtcgatcgc gttcttggat ccgcggtcga gccacacgga gccgaccacg acgactcccg
     5161 cctcgccgag caagtgcgtc gattcgagaa ggatcggatc gaggagacgc tccgcctcga
     5221 agggggcaac gtcaccaagg cagccgcgcg tctcggcctc gagcggagcc acctctacaa
     5281 gaagatgcgc aagctcggga tcgaatccgc ctagacgcct cccgctgtcc cccccatctc
     5341 cggtccggaa cttgcatttt gccctcccgc cgagagggct gcgtagcggc ccgcgccgat
     5401 ccatgccgaa gggggacgcg tatctcccac attcggcacg agttcgctcg acatcgatgg
     5461 ccccggggcc gctgcgccaa gcgcccggtc ctggcggacc ccgtacccaa ttgttccaag
     5521 ggggacgtca tgagaagccg attcgaggaa tacaacgagg acgaccatct ggacgaaccg
     5581 gtcaccatca cgggtcgcca gcttcgttcc ctcaagcgcg ccgccacgac cgggttgatc
     5641 gcgacgatcc tcgccatcat cgccgtcgga ctgggcgctt ggaatctcgt gcaggcgggc
     5701 aagagcggtc cgtcggatcc catgagctcg gagccggccg cgcaagctca gcctgcgccg
     5761 gcggccgcgg cgaccgactc cgtgccgtcg ccagtgccga ccccgcagcc aatccagaca
     5821 ggtgccgctg cgacgcccgc gccatcaacc gcgccggccg ccgccaccgc gacaaagccg
     5881 gccgccgccg caaaggcccc acagcacgca tcggcagccg cgccggcgag gcattcacgc
     5941 tcgtccagga gcgcggcgtc gagcggcgaa gagccggtga ccacgcctgc gttctcaccg
     6001 tccgcggctc cggtctcgcc cgcgatgccg atcccgaccc cggcgccctc gcctgtcgca
     6061 ccgaagccgg cggcgccgga cacgaccaag cactaggcgc gcggcccact ccgtactccg
     6121 tgcttgagca agcccgcgag gcggggctag catgccccat acacggaggg gccgatgccg
     6181 atcgaatacg agatcgacca tacccggcgg atcgtgatcg cgcgggggag cggtgtcctc
     6241 ggagaagaag acttcttcga gtaccagaag gacgtctggt cgcgggacga agtccgg
//
LOCUS       DASTKC010000024        11845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_19714, whole genome shotgun sequence.
ACCESSION   DASTKC010000024 DASTKC010000000
VERSION     DASTKC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19714"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFR25_00870"
     CDS             <1..465
                     /locus_tag="VFR25_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939631.1"
                     /translation="RYPKDSLSVVLFGDDAWEVKIRDIAYATVGPFHTNTKAGLQLAQ
                     KILARQKHVNKQIFMITDGKPSAIFENGRFYKNAFGLDPRIVNKTLDEAVACRRKRIP
                     ITTFMVTQDPYLVRFVERFTQLNKGRAYFSDLEELGSYLFVDYAQNRKRRVQ"
     gene            1407..2288
                     /gene="hpnC"
                     /locus_tag="VFR25_00875"
     CDS             1407..2288
                     /gene="hpnC"
                     /locus_tag="VFR25_00875"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HET9939632.1"
                     /translation="MTQIPAEAPAAPEPVPLSDAYRVCADIVRRHDENFPVVSRFIAT
                     PLRPALAAVYAFARLADDVADAAAPSAERLDGLDRIEAALLEALDGDPKGPVLTALAD
                     AVDQHSLPVEPFLDLLGAFRMDARDATFETWDDLLGYCRGSANTIGRLVLALHAIDDP
                     TTLAESDDVCTALQLTNLWQDLARDLERGRVFLPRADMARSGLEAGTLRDPDRQDALT
                     QLLREECRATEGLYARGEAIPTRVPRGLGIQLRMTLAGGRAVLRRVEERGWRVLVDRP
                     SLGQADRLKLLVKGVFA"
     gene            2285..3163
                     /gene="hpnD"
                     /locus_tag="VFR25_00880"
     CDS             2285..3163
                     /gene="hpnD"
                     /locus_tag="VFR25_00880"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET9939633.1"
                     /translation="MSARAAESVDARSRSTTRPTPPGRRSHFGVAFAILPASQRDAIR
                     AVHAWSHAVDDAVDEERDPERARDSVERWRRDVERLYDGAEPDEPETRALRSHVDRFH
                     IPRGYLDELLRGIEMDLTHARYHTFQDLAGYCYRVASVVGLICLRIFGDEEERGRAYA
                     EDLGMALQLTNILRDVGSDHARGRIYLPAEDRERFGYTEEALGRRERNEAFFELMRFQ
                     AARAKSFFAAADREALQLDRKRLIAAEIMGRVYRRLLERIEASGFDVFAREIRVPRME
                     RVWIAASTAVAIRAAR"
     gene            3160..4524
                     /gene="hpnE"
                     /locus_tag="VFR25_00885"
     CDS             3160..4524
                     /gene="hpnE"
                     /locus_tag="VFR25_00885"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HET9939634.1"
                     /translation="MSTPQSRPQTVRPVRSPITNPDVIVVGAGFAGVAAATALAQRGA
                     HVLVLETRQRPGGRAYSWTDPTTGEVRDNGQHVLASFYDETHRLLARLGTADALQADH
                     AFRLRVEVRLRGGYELACPSWPAPWSWAGGILGASGLSLPSRLAALGLQGRIRSLLAR
                     NGSSADTTIQSWLSDLGRGTEDLEGLLWALAISALNDLPGEASATLFARVLGRLLAAP
                     TERTGLAMPRRALGDLIAGYEDFLAQRGSEVRYRTTALGVRIDGGRATGVSLLGGTRL
                     DAGAVILTVPHERITWMLRPEFAEPYRAIAAVPWSPIVSTMHVFDRPVLPERMVALLG
                     TRTQWAFDRGEAGAGRFRVGTVRSAAFADAERDVDVIAREAEAELLESFPAARDARLL
                     ETRVYKERRATMRSTPAAQRVRPVAETAIQGLYLAGDWTDTGLPPTIEGAVLSGHRAA
                     ELAR"
     gene            4521..5570
                     /gene="mutY"
                     /locus_tag="VFR25_00890"
     CDS             4521..5570
                     /gene="mutY"
                     /locus_tag="VFR25_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9939635.1"
                     /translation="MSARNATVKRPTAPRPKRIRPALLAWYRAQRRDLPWRRTRDPYA
                     IWVSEIMLQQTRVETVIPFYERFLERFPTVNALASAHDEDVLAAWSGLGYYRRARGLH
                     AAASIVAREHAGVLPMDSERLRALPGIGEYTAAAIASIAFGQETAAVDGNVVRVLARV
                     HGLRGSRDSARLKREVTHLATALARGPAPGDWTQAVMELGAVVCLPRPKCDECPVARS
                     CDAHRSGDASRYPAPAKKPDPRAAGEVLLLARRGQSIYLERAATPTGTAWTLPRARTR
                     EEAARLGFELRSGDPLTSFRHRTYAEDLKIEVCAAKAGRRGAQGRWFAPDALDRLPLR
                     APTLKAIKRLRLLSS"
     gene            5822..8155
                     /locus_tag="VFR25_00895"
     CDS             5822..8155
                     /locus_tag="VFR25_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9939636.1"
                     /translation="MVDLMIEGDIAPGLLRARGIEVNTVVGRRMTARCPLGLLPAFLA
                     MPGIDRVEVSERCEPTLDSMAVETGIATVRTVTPPTITGQTGAGVLIGIVDTGVDLTH
                     PDFKNEDGTTRLVSVWDQTVAGTPPAGFTYGTEWSHAQIQSGVATEVDDEGHGTHVLG
                     IIGGDGSTTGNGQPAFRYVGVAPAADLCVVKTDFGTTDIVDGVAYIFQVAAARGQKAV
                     VNMSLSSQAGPHDGTYAFDTMVNDLTGPGKIVVTSAGNQQTAAIHAQAAVGGSPVTLS
                     MVVPSYTRNSGPANDYLLFTGWYEGADQMSITVISPNGNTVGPVTPGTDHSDNNTPDG
                     NITVFNGTTTPSNGDHEIYVQLYDSQANRAPATGTWQFVLTPISIQSTGRFDMWLYSN
                     TLGPGASSPQWVNGLAPNTVVGTPASADSVIGVAAHVTKNCWVGSDGIGYCWNPTPAI
                     GSIAYFSSTGPRRDGVIKPDISAPGFGVTSALSAATTPTPDPALVMLDGKHLIEAGTS
                     MSSPIVAGTVGLLLAQPDWANASPSAIKARLKATARADAFTGSVPNSVWGAGKLNAAA
                     ALAPLFALHIQHPAKGQYIPPGKPDSVQVVVGGATAESVAFALSLDGGGTYPIPLGTL
                     TAVVPGPPRSLAWWVDGSYATSAAKIRGIAYRAGSNLTAYSDSLFLIAAPTAVEIADA
                     TPAARFSLSPNVPNPFNPETMIRFATANAGRVTLIVYDIHGARVRALVNEWMPAGSFR
                     ARWDGRNDHGTAVASGVYLYELREGGNRISRKMSLLK"
     gene            complement(8186..10174)
                     /locus_tag="VFR25_00900"
     CDS             complement(8186..10174)
                     /locus_tag="VFR25_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9939637.1"
                     /translation="MSRGTTDLTAGASTEQTVRGRALIFWDPKRPGKKLDAIDTDQIT
                     PAEDCVSESLETLDERWKAGAFRHLMPGFRARVHAGETFVVAGDRFAIGSSREMSPAG
                     LKAIADEAGLTMVVVCGANMGDIFRRNALNLGLHVVQCIEAVEDARDGDVMEFDVESR
                     SIRNVTQGKSYESKPLTAKEEEIRHSGGIIAVGRREMAEARGRKPQVEFPDPFRARYQ
                     TSTEQILWAHRVDKKADVKPGATIQVYADLLPASDGTAPFAIHTFQQIVGDPDVKPRL
                     AAVANDHFVFTGKDADQKQTSISKAFAEKHDIRAPYYASPGTGIFHFYFPEQGLIVPG
                     GFYPGADSHSRAYGAYGAVGIGVGSTTLGFGWATGTIFVTVAKQRRVVFNGKLKPWVS
                     GKDIVLRLLQGWGVKQSQGMSVEFVDRNGELPIPYRNTIANMMAEGEALNGIFAQDAV
                     TEAWYKARGFPLPYPRVTPGEDARYELDETLDLSSVAPMIAKPFSPGNAFPAEEVATE
                     RLTFHKAMIGSCTNGGYDDLLQAALVLRGARTAGIERARRDVEFIVFPGSGTVKKLIE
                     RPDPRLGGESIADVFRAAGAEIRESWCGPCFGQGPDSLEPGERAITTFNRNWQNRMGV
                     GGEGYLASPAVVAASALAGYMAPPSELGLRWDPEAYGI"
     gene            complement(10171..>11845)
                     /locus_tag="VFR25_00905"
     CDS             complement(10171..>11845)
                     /locus_tag="VFR25_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939638.1"
                     /translation="SLQVVEAGMDARRLAPRTAAEAPQFPAARIRRPELRFRRLTFME
                     GEIGVARFTPDGQTVVYSASWDGGPPQLYLSRVGSVETRPLGVRDCEVLAVSKRDEVA
                     VMKNPRDIGGFVVVGTLARVPLIGGTPREMLDRVVRADWSPDSRSLLVLRAIDGRYRL
                     EYPIGTVLHEAEGWMSDPRLSPDGTMIAFREHPVRADNGGYLKVIDLENRVIAQSEYF
                     WTAWRHAWDPSGQAIWFSAAGDERSPGIYRMTLDGATEPVYQAPTYTTVEDVSPRGDL
                     LSLSVIPRMRMQGGSKDGGETRDLAWLDWSLVRDISSDGEWVLFGESGVTGGGPGVYI
                     RRLDGTPAIRLGDGDCWRLSPDFQWVLITQSGRHDDFALLPTGAGPLVPLPTLGLRCS
                     NATWFPDGTGFIALAAEKGHGQRLYRYDLETKTVHLIRDEAFHQIGSISPDGRQLLAM
                     DHERRTVIVATDGSSIRRIGPAVEGRRPICWNREGTHFFTYNKGTIPAPVFLVDAETG
                     EETLWLTIETRERHGVQSVNNVCLTPDGMTYVASYPQTLSSLYVMQEVS"
BASE COUNT         1823 a         4200 c         3933 g         1889 t
ORIGIN      
        1 cgatatccca aggactcgct ctcggtcgtg ctttttgggg acgacgcgtg ggaggtgaag
       61 atccgcgaca tcgcgtacgc gacggtggga cccttccaca cgaacacgaa ggcggggctc
      121 cagctcgcgc agaagatcct cgcgaggcag aagcacgtga acaagcagat cttcatgatc
      181 acggacggca agccgtccgc catcttcgag aacgggcgct tctacaagaa cgcgttcggc
      241 ctggatccca ggatcgtgaa caagacgctg gacgaggcgg tcgcctgcag gcggaagcgg
      301 atcccgatca ccacgttcat ggtgacgcag gatccatatc tcgtccgctt cgtcgagcgg
      361 ttcacccagc tcaacaaggg gcgcgcttac ttctccgatc tcgaggagct cggctcctac
      421 ctcttcgtgg actacgcaca gaatcggaag cggcgggtgc aatagcggag tccggcgggg
      481 cggccggcgc ctgctcgggc caccatggcg cggagagcgg atcgtgtggc gcgggcgctc
      541 gttcaggctc ctccacgcga gtgctccggt gaatgaacct gtaagaaagg gacaccgcga
      601 aagcgttctc gctcaccccc tcggagctcc ccaggcgcag cgttcccccg acccctaggc
      661 gatcgccgaa tcggtactca ccccgcccca cgaatcccgg catgtcttgc gggaccgcgg
      721 ccgcgccgag ccagaaatcc attcgtggcc gccggaagcc aaagcccatc tgcccatatc
      781 cagtggtgag gagcggcacc gcctcgaagt agctgaactc gaagtaggtt cccttcccga
      841 cccggatgtg accgctcccg gtcttgctca agtccacctc cgggaaatac tggtcccctt
      901 tcaccaagcc cccgccgatc gagaaactcg gccagtcgaa cgacaggtag ggattcacgt
      961 accggacgtc gtacgacgac tcgtacccat gccgtcctgc ggccgcgggt ctcacgatcc
     1021 cggcgcgcat gccgaggctg accggggccg tgaatttgtg gctgacctcg gcgccgacgt
     1081 tgtcgtagtc gatcttccgc ttgctgatca cgctgttgtc gcatccgcgg gtgacgtagg
     1141 cataggaacc agagccgccg gcgatacgca gcgccgtgac actgctgtct gtgtggctga
     1201 ccgcgagcgg agccgccgcc agagcggcac tcaagagcgg tacggaccga cgcatgctga
     1261 gctcacctcc tcggagccgg accaggcggc tccaagccca agaacgcggg gactctcgtg
     1321 aaacgcacaa cgtcccgccg aggttccccc gccttgacgc tccgaatgac gcatgttcta
     1381 ctccttcaac cacctgagga cgccgcgtga cgcagatccc agccgaagcg cccgccgcac
     1441 ccgagcccgt gccgctttcc gacgcctacc gcgtgtgcgc ggacatcgtc cggcggcatg
     1501 acgagaattt cccggtcgtg tcccggttca tcgcgacgcc cctgcgcccc gcgctcgccg
     1561 cggtctacgc gttcgcgcgc cttgccgatg acgtggccga cgcggcggcg ccctccgccg
     1621 agcgcctcga cggactcgat cgcatcgagg ccgcgctcct cgaagcgctc gacggagacc
     1681 cgaagggtcc cgtgctcacg gcgctcgccg acgcggtgga ccagcactcg ctcccggtcg
     1741 agccgttcct cgatctcctc ggggcgtttc gtatggacgc gcgcgacgcc acgttcgaga
     1801 cctgggacga tctcctcggc tactgccgcg gctcggccaa cacgatcggc cggctcgtgc
     1861 tcgcgctcca cgcgatcgac gatccgacga cgctcgcgga gtcggacgac gtctgcacgg
     1921 cgctccagct cacgaatctc tggcaggatc tcgcgcgcga cctcgagcgc gggcgcgtgt
     1981 tcctccctcg cgcggacatg gcacggtccg ggctcgaggc cggcacgctt cgtgatccgg
     2041 atcggcagga cgcgctcacc cagctcctcc gcgaggagtg tcgcgcgacc gaaggcctct
     2101 acgcccgcgg cgaagccatt cccacgcgcg tgccgcgcgg gcttgggatc cagctccgca
     2161 tgacgctcgc cggcggacgc gccgtgctcc gccgcgtcga agagcgcgga tggcgcgtgc
     2221 tggtcgaccg cccgtcgctc ggccaggccg atcgcttgaa gctcctcgtg aagggagtgt
     2281 tcgcgtgagt gctcgggcgg ccgagtccgt ggacgcgcgg tcccgctcga ccacacgacc
     2341 gaccccgccc ggacgccgct cccactttgg ggtcgcgttc gcgatcctgc cggcgtcgca
     2401 gcgcgacgcc attcgcgccg tccacgcctg gagccatgcg gtggacgacg cggtcgacga
     2461 ggagcgcgat cccgagcgcg cgcgcgattc cgtggagcgc tggcggcgtg acgtggagcg
     2521 tctctacgac ggtgcggagc cggacgagcc ggagacgcgc gcgcttcggt cccatgtcga
     2581 tcgattccac attccgcgcg gttacctgga cgagctcctc cgcggcatcg agatggatct
     2641 gacgcatgcg cgctaccaca cgttccagga cctcgcgggt tattgctacc gcgtggcctc
     2701 ggtcgtgggg ctcatctgcc ttcgcatctt cggcgacgag gaggagcgtg ggcgcgcgta
     2761 cgccgaggat ctcggcatgg cgctccagct caccaacatt ctccgcgacg ttggctccga
     2821 ccacgcgcgc ggccgcatct atctgccggc ggaggatcgc gagcggttcg gctacacgga
     2881 ggaagcgctc gggcgccgcg agcggaacga ggccttcttc gagctgatgc gcttccaggc
     2941 cgcacgcgcg aagtccttct ttgccgccgc ggaccgagag gcgctgcagc tcgatcggaa
     3001 gcgactgatc gcggcggaga tcatgggccg cgtgtatcgc cggctcctcg agcggatcga
     3061 agcctcgggg ttcgacgtct tcgcgagaga gatccgcgtc ccgcgcatgg agcgcgtctg
     3121 gatcgccgca tccaccgcgg tcgcgatccg cgcggctcgg tgagcacgcc tcagagccgt
     3181 ccgcaaaccg tgcgccccgt tcgttcgccg atcacgaatc ccgatgtcat cgtggtgggc
     3241 gccggatttg ccggtgtggc ggccgcgacc gcgctcgccc agcgcggcgc tcacgtcctc
     3301 gtcctcgaga cccggcaacg ccctggggga cgcgcctact cctggaccga tcccacgacc
     3361 ggcgaagtgc gggacaacgg gcagcacgtc ctcgcttctt tttacgacga gacccatcgc
     3421 ctgctcgccc gcctgggcac ggcggacgcg ctccaggccg atcacgcatt ccggcttcgc
     3481 gtcgaggtgc ggcttcgagg cggctacgag ctcgcgtgcc cgtcatggcc cgcaccgtgg
     3541 agctgggccg gcggcatcct gggagcatcg ggattgagcc taccgtcgcg cctggccgca
     3601 cttggacttc aaggacggat ccgctcgctt ctcgcacgca acggatcgag cgccgacacc
     3661 acgattcaga gctggctgtc cgatctcggc cgcggcacgg aggatctcga agggctgctc
     3721 tgggccctcg cgatctccgc gctcaacgac ctcccgggcg aagcctcggc gacgttgttc
     3781 gcgcgcgtcc tcggccggct ccttgcggcg ccgacggagc gcaccgggct tgcgatgccg
     3841 cgccgcgcgc tcggcgactt gatcgctggg tacgaggatt tcctcgcaca gcgaggcagc
     3901 gaggtccggt atcgcacgac cgcgctgggc gtgcggatcg atggcggccg ggccacgggc
     3961 gtctcgctcc tcggcggaac gcgcctggat gccggcgcgg tgatcctcac ggtgccgcac
     4021 gagcggatca cgtggatgct tcggcccgag ttcgcggaac cgtatcgcgc catcgcggcc
     4081 gtgccctggt ctcccatcgt ctcgaccatg cacgtcttcg accggccggt gcttcccgaa
     4141 cggatggtgg cgctcctcgg aacgcgcacc cagtgggcgt tcgatcgcgg cgaggcggga
     4201 gcgggacggt tccgggtggg aaccgtgcgg agcgccgcgt tcgcggacgc ggagcgcgac
     4261 gttgatgtca tcgcgcgcga ggcggaggcc gaactattag agtcattccc ggctgcgcgt
     4321 gatgcgcgcc tcctcgagac gcgcgtgtac aaggagcgcc gcgccacgat gcgatccacg
     4381 ccggcggcgc agcgcgtgcg tcccgtggcg gagaccgcga tccagggcct gtatctcgcg
     4441 ggcgattgga ccgacacggg actcccgccg acgatcgagg gagccgttct gagcgggcat
     4501 cgcgcggccg agctcgcccg atgagcgccc gcaacgccac cgtgaagcgc ccaaccgcgc
     4561 ctcgtcccaa gcgcatccgt cccgcgctcc tcgcctggta ccgcgcgcag cgccgcgacc
     4621 tcccctggcg ccgtacgcgc gatccctatg cgatctgggt gagcgagatc atgctgcagc
     4681 agacgcgggt cgagaccgtg atccccttct acgagcgctt cctcgagcga ttccccaccg
     4741 tgaacgcgct cgcgagcgcc cacgacgagg acgtgctcgc cgcatggtcc ggactcggct
     4801 attaccggcg cgcgcgcggg ctccacgccg cggcatccat cgtcgcgcgg gagcacgccg
     4861 gcgtgcttcc catggattcc gagcggctgc gcgcgctccc gggcatcggc gagtacaccg
     4921 ccgccgccat cgcgagcatc gcgttcggac aggaaaccgc ggcggtcgac gggaacgtgg
     4981 tgcgcgtgct cgcgcgcgtc catgggttgc gcggcagccg cgactccgcg cgtttgaagc
     5041 gggaggtcac ccatctggcc accgccctgg cccgcggccc agcgccgggc gattggaccc
     5101 aggccgtgat ggaactcggg gcggtcgtct gccttccccg gccgaaatgc gacgaatgcc
     5161 ccgtcgcgcg ctcctgcgac gcgcaccgga gcggcgatgc ctcgcgctat ccggcgccag
     5221 ccaagaagcc cgacccgcgc gcggcgggcg aggtgctcct cctagcgcga cgcggccagt
     5281 cgatctacct cgagagagcg gccactccaa ccggcacggc gtggacgctt ccgcgcgcca
     5341 ggacgcgcga ggaagccgcg cggctcggct tcgagcttcg atcgggcgat ccgctcacgt
     5401 ccttccgcca ccgcacgtac gccgaagatc tcaagatcga ggtctgcgcg gcgaaagccg
     5461 gccggcgcgg cgcccagggg cggtggttcg ccccggatgc gctggaccgg ctgccgttgc
     5521 gtgctcctac cctcaaggcc atcaagaggt tgcggctgct aagttcttga tttttcaggc
     5581 gtttcggggt atacttggtg tgtcttcgca tccttgcggt tcgacgcgcc ttggggactc
     5641 gatgcgccac gctgctccta ctccgctcct cctgttggcg ctaatagccg cccccctttc
     5701 gatcgccttc gtatccacgg cgcacgctgc ccgctccgtc gctccttcgc tgacccggcc
     5761 catcgatcca acgctcccca tccatcagcg cttcctatcg aaggacgccc atggagcgcc
     5821 gatggtggat ctcatgatcg agggggacat cgcgccggga cttctccgcg cgcgcggcat
     5881 cgaggtgaac acggtggtcg gacgtcggat gaccgcacgc tgccccctcg gcctgttgcc
     5941 tgcgttcctc gccatgccgg gcattgatcg cgtcgaggtc tcggagcgct gcgagcccac
     6001 gctcgactcc atggccgtcg agaccggcat cgccaccgtg cgcacggtca cgccaccgac
     6061 gatcacgggg cagaccggtg caggcgtcct gatcggcatc gtggataccg gcgtcgacct
     6121 cacccatccc gacttcaaga acgaggacgg cacgacgcgg ctcgtgtccg tgtgggacca
     6181 gacggtggcc ggcacgccgc cggcagggtt cacctacggc acggagtggt cgcacgcgca
     6241 gatccagtcc ggagtcgcga cggaagtcga cgacgaaggg cacggaaccc atgtgctcgg
     6301 aatcatcggc ggcgatggaa gcacgaccgg caacggccag cccgcgttcc gctatgtggg
     6361 cgtggcacct gccgctgacc tctgcgtcgt gaagaccgac ttcggcacga ccgacatcgt
     6421 cgacggcgtc gcctacatct tccaggtggc cgccgcgcgg ggacagaagg cggtcgtcaa
     6481 catgagcctg agctcgcagg cagggccgca cgacggcacc tatgccttcg acacgatggt
     6541 caacgacctc accgggccgg gaaagatcgt cgtcacttcc gccgggaacc agcagaccgc
     6601 cgccatccac gcgcaggcgg ccgtcggcgg ctcgcccgtg actctctcga tggtggttcc
     6661 gtcctacacg aggaactcgg gccccgcgaa cgactacctc ctgttcacgg gctggtacga
     6721 gggggcggat cagatgtcga tcacggtcat cagcccgaac gggaatacgg tgggcccggt
     6781 gactccgggc acggaccaca gcgacaacaa cacgccggac ggcaacatca cggtcttcaa
     6841 cggcaccacg accccttcga acggcgacca cgagatctac gtccagctct acgactcgca
     6901 agccaaccga gcccccgcga ccggcacatg gcagttcgtg ctgacgccga tttcgatcca
     6961 gtcgaccgga cgattcgaca tgtggctcta ctccaacacg ctcggcccgg gcgcctcgtc
     7021 accacagtgg gtgaacgggc tcgcgcccaa caccgtcgtg ggaacgcccg ccagcgcgga
     7081 ctccgtcatc ggagtcgccg cgcacgtgac aaagaactgc tgggtggggt cggacgggat
     7141 cgggtactgc tggaatccaa cgcccgccat cggatcgatc gcgtacttct cttccaccgg
     7201 acctcgacgt gacggcgtca tcaagccgga catctcggct ccgggattcg gggtgacgtc
     7261 cgcgctgtcc gccgccacca caccgacgcc cgacccggcg ctcgtgatgc tggacggcaa
     7321 acacttgatc gaggctggca cgagcatgtc gtcgcccatc gtggccggta cggtcgggct
     7381 cctcctcgcg cagcccgact gggcgaacgc aagcccgtcc gcgatcaagg cgcgcctcaa
     7441 ggcgaccgcg cgcgcggacg cgttcacggg cagcgtgccg aactcggtct ggggggccgg
     7501 caagctgaac gccgctgccg cactcgcgcc gctcttcgcg ctccacatcc agcacccggc
     7561 gaaagggcag tacattcccc cagggaagcc tgactccgtg caggtggtgg ttggcggcgc
     7621 caccgccgag tcggtggcgt tcgcgctctc gctcgacggc ggagggacct acccgatccc
     7681 gctcgggacc ctcacggccg tggtccccgg cccgccgcgt tccctggcgt ggtgggtgga
     7741 cggctcgtat gcgaccagtg cggccaagat ccgcggcatc gcgtatcgcg ccggaagcaa
     7801 cctcaccgcc tactcggaca gcctcttcct catcgccgcg cccaccgcgg tcgagatcgc
     7861 ggacgccacg cccgcggccc ggttctcgct ctcgcccaac gtgccgaatc ccttcaatcc
     7921 ggagacgatg atccgattcg ccacggcgaa cgcggggcgc gtgacgctga tcgtgtacga
     7981 cattcacggg gcgcgcgtgc gcgccctggt gaacgagtgg atgccggccg ggtcgttccg
     8041 cgcgcgctgg gatggacgga acgatcacgg gacggcggtc gcgtcggggg tgtatctcta
     8101 cgagctgcgc gagggcggga accggatctc gaggaagatg agcctgctga agtaactccg
     8161 cggcgcaggg cgcgccgcga tgacgctaga tcccgtacgc ctccggatcc cagcgaagtc
     8221 ccagctccga cggcggcgcc atgtagcccg cgagcgcgga cgcggccacg accgccgggc
     8281 tcgcgagata gccttccccg ccgacgccca tccggttctg ccagttccga ttgaacgtcg
     8341 tgatcgcgcg ctcgcctggt tcgagcgagt cgggaccctg accgaagcag ggaccgcacc
     8401 aggactcacg gatctccgct ccggccgccc ggaacacgtc cgcgatcgac tctccgccga
     8461 ggcgcgggtc gggccgctcg atcagcttct tcaccgttcc gctccccggg aagacgatga
     8521 attcgacgtc gcgccgagcc cgctcgatac ccgcggtgcg tgcgccgcgc agcacgagcg
     8581 ccgcctggag caggtcgtcg tagccgccgt tcgtgcagga gccgatcatc gccttgtgga
     8641 acgtgagccg ctcggtcgcg acctcttcgg ctgggaacgc gttccccggg ctgaagggct
     8701 tcgcgatcat gggggcaacc gaggagagat cgagcgtctc gtccagctcg tagcgcgcgt
     8761 cctcgccggg ggtgacgcgc ggatacggga gcgggaaccc gcgcgccttg taccaggcct
     8821 cggtcaccgc gtcctgcgcg aagatgccgt tcagcgcctc cccttcggcc atcatgttcg
     8881 cgatcgtgtt ccggtacggg atcggcagct cgccgtttcg atccacgaac tcgaccgaca
     8941 tcccctgtga ctgcttcacg ccccatccct ggaggagccg gaggacgatg tccttcccgc
     9001 tcacccaagg cttcagcttc ccgttgaaca cgacgcggcg ctgcttcgcc accgtgacga
     9061 agatggtccc cgtcgcccag ccaaacccga gcgtcgtgct ccccacgccg attcccacgg
     9121 cgccgtacgc gccgtacgcg cggctgtgcg agtccgcgcc cgggtagaag ccgccgggga
     9181 cgatcagccc ctgctccggg aagtagaagt ggaagatgcc cgtgccgggc gacgcgtagt
     9241 agggcgcgcg gatgtcgtgc ttctccgcga aggccttcga gatcgacgtc tgcttctggt
     9301 ccgcgtcctt gccggtgaag acgaagtggt cgttcgcgac cgcggcgagc ctgggcttca
     9361 cgtccggatc gccgacgatc tgctggaacg tgtggatcgc gaacggcgcc gtgccgtccg
     9421 aagcgggcag gagatcggcg tagacctgga tcgtcgcgcc cggcttgacg tcggccttct
     9481 tgtcgacgcg atgcgcccag aggatctgct cggtggaggt ctggtagcgc gcacggaagg
     9541 gatcggggaa ctcgacctgc ggcttccgtc cacgcgcctc ggccatctcg cgccggccga
     9601 ccgcgatgat gcctccgcta tggcggatct cctcttcctt cgccgtgagc ggcttcgact
     9661 cgtagctctt cccctgggtc acgttccgga tcgagcggga ctcgacatcg aactccatca
     9721 cgtcgccatc gcgagcgtct tccaccgcct cgatgcactg gacgacgtgg agtcccaggt
     9781 tgagcgcgtt ccgccggaag atgtccccca tgttggcgcc gcacaccacg accatcgtga
     9841 gccctgcctc gtcggcgatc gccttcaagc cggccggtga catctcgcgc gaggagccga
     9901 tggcgaatcg gtcgcccgca acaacgaacg tctcccccgc gtgcacgcgc gcgcggaagc
     9961 cgggcatgag atggcggaac gcgccggcct tccaccgctc gtcgagggtc tcgaggctct
    10021 cggagacgca gtcctcggcg ggggtgatct gatccgtgtc gatggcgtcg agcttcttgc
    10081 cggggcgctt tggatcccag aagatgagcg cgcgcccgcg caccgtttgc tcggtgctcg
    10141 cgccggcggt caggtccgtc gtgccccgac tcacgaaacc tcctgcatca cgtagagact
    10201 cgagagggtt tggggatagc tcgccacgta ggtcatccca tccggggtca ggcacacgtt
    10261 gttcacgctc tgcacgccgt gccgctcgcg cgtctcgatc gtgagccaga gggtctcctc
    10321 tccggtttcc gcgtcgacga gaaagaccgg agcggggatc gtacccttgt tatacgtgaa
    10381 gaagtgggtt ccctcccggt tccagcagat cgggcggcgg ccctccaccg ccggtccgat
    10441 cctccggatg gagcttccgt cggtggccac gatcaccgtc cggcgctcgt ggtccattgc
    10501 gaggagctgg cgcccgtccg gagaaatgga cccgatctga tggaacgcct cgtcgcggat
    10561 cagatgcacc gtcttcgtct cgagatcgta ccggtagagg cgctgtccgt gtcctttctc
    10621 ggcagcgagc gcgatgaagc ccgttccgtc tgggaaccag gtggcgttgg agcatcggag
    10681 cccgagggtc ggcaggggca cgagggggcc cgcccccgtc ggaaggagcg cgaagtcgtc
    10741 gtgccgtccg ctctgtgtga tgagcaccca ctggaaatcg ggcgaaagcc gccagcagtc
    10801 gccgtccccc aatcggatgg cgggggtgcc gtcgagccgg cggatgtaga cgccggggcc
    10861 gccaccggta acccccgatt ccccgaagag gacccactct ccgtccgagg agatgtcgcg
    10921 gacgagtgac caatcgagcc aggcgagatc tcgcgtctcc cctccatctt tggagccgcc
    10981 ttgcatgcgc atccggggaa tcaccgacag gctcagaagg tcgccccgcg gcgaaacgtc
    11041 ctcgacggtg gtgtaggtag gcgcctggta cacgggctcg gtcgcgccgt cgagcgtcat
    11101 gcggtagatc ccgggcgagc gctcgtctcc cgcggccgag aaccagatgg cctggcccga
    11161 cgggtcccag gcgtggcgcc aggcggtcca gaagtactcg gattgcgcga tcacgcggtt
    11221 ctcgagatcg atcaccttga ggtagcctcc gttgtcggcg cgaaccgggt gctcgcggaa
    11281 ggcgatcatc gttccatcag gggacaacct cggatcgctc atccagccct ccgcctcgtg
    11341 caggacggtc ccgattggat actcgagccg atacctcccg tcgatggcgc ggagcacgag
    11401 gagcgagcgg ctgtccgggc tccagtccgc gcgcacaacc cggtcgagca tctcgcgcgg
    11461 cgtgccgccg atgagcggca cgcgggccag ggtgcccacg acgacgaacc cgccgatgtc
    11521 gcggggattt ttcatcacgg cgacttcgtc ccgcttcgac acggcgagca cctcgcaatc
    11581 gcgcacgccg agcggcctcg tttccacgct ccccacccgt gacaggtaga gctgtggggg
    11641 ccctccgtcc cacgaggcgc tgtacacgac ggtctggccg tcgggggtga agcgcgctac
    11701 tccgatttcc ccctccatga aggtgagccg gcggaagcgg agctccggac ggcggatcct
    11761 cgcagcggga aattgcggcg cttctgccgc cgtgcgtggg gcgagcctcc gcgcgtccat
    11821 gcccgcctcc accacctgga gcgag
//
LOCUS       DASTKC010000025         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_492889, whole genome shotgun sequence.
ACCESSION   DASTKC010000025 DASTKC010000000
VERSION     DASTKC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492889"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1633)
                     /locus_tag="VFR25_00910"
     CDS             complement(5..1633)
                     /locus_tag="VFR25_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9939639.1"
                     /translation="MKGFIPARGIVCALVAIGLVSLLVAQPASAGVNFTSGKVYVQQK
                     VYDKAAHYLELARKEEPDNTQVYSLLGFSRSQLRQFASAGGAFQIGIKVCTDKKDKKR
                     ADEIEQNRKALYADLFNQGIKALGRAGKIAQDDARTTDEGTPQAAVAKERGEPRDFSR
                     FTENGKMQEFWYYPDQSTVYYFPQGADPIQIPYKPFSVSADPSVAVTDTTVFPAYTGG
                     SAVAEASYDFGLAMLIDPSSPDTYKNLSYTYDLLGRPDDAIKAAQRGLELKPGDEQLT
                     RNLRVAAMGRGNRLYGSGRFMEAIPAYRAAMAYDAAGTVQYLSLIAECYQKGAPSEGP
                     TRAALLDSASAAYMEVYNKAPNDSTGASLKENAIYNSAIIQLNLENTKKGLDILNQGV
                     AAFPNNKDLLTLQGQTKFQAGDMPGAVEAMQKVVTIDPKSADAHQVLFSAYNKLKQQD
                     KSVAEYTIYKALSDGKQRTGSQLKVWVDSAPNRLGAKEQLTKTKTTEGYPEEVRTFMD
                     GDKNLESWFYWSKGKSITFFEGQLFSQATFPPAH"
     gene            complement(1740..>2760)
                     /gene="priA"
                     /locus_tag="VFR25_00915"
     CDS             complement(1740..>2760)
                     /gene="priA"
                     /locus_tag="VFR25_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET9939640.1"
                     /translation="AEAPPVQPSSAYLTPALLTAMERTLVRGEQGILFLNRRGHSTVL
                     QCRGCGSVASCARCDVSLTVHTEDQTLRCHYCGATRRLLLSCKECGASDLWLGGVGIQ
                     KIEREVARLFPHARLARLDFDAVRKRGAAGGILRSFRQGDTDFLLGTQMVTKGFDFPG
                     VTLVGIIVADLQLFLPDFRAAERTFQLLTQVAGRAGRGKSPGEVIMQSYNPDHPALRC
                     AADQDFEAFFALEAAERRELGYPPYGHLVAVEIRGATLDRVVEAAQRVRKALARIQPA
                     VEILGPAPKPISRIQGKERWHLLLRSPSRKSLREHLKRGIPAIRGLKLPGVHVAVDVD
                     PRQLL"
BASE COUNT          437 a          889 c          927 g          507 t
ORIGIN      
        1 tgacttagtg ggcgggcggg aacgtggcct gggagaagag ttggccctcg aagaatgtaa
       61 tcgacttgcc cttggaccaa tagaaccagc tctccaggtt cttgtcccca tccataaagg
      121 tccggacttc ctccggatag ccctcggtgg tcttggtctt cgtgagctgc tccttcgccc
      181 cgagccggtt cggagctgaa tccacccaca ccttgagctg ggatccagtg cgctgcttcc
      241 cgtcactcag ggccttgtag atggtgtact ccgccaccga cttgtcctgt tgcttcagct
      301 tgttgtaggc ggagaagagc acctggtgcg cgtccgccga cttgggatcg atggtcacca
      361 ccttctgcat ggcctcgacc gcccccggca tgtcgccggc ctggaacttg gtctggccct
      421 ggagggtgag caggtccttg ttgttcggga aggcggcgac accctggttg aggatgtcga
      481 gacccttctt cgtgttctcg agattcaact ggatgatggc cgagttgtag atcgcgttct
      541 ctttgagcga ggcccccgtc gaatcgttgg gcgccttgtt gtagacctcc atgtaggcgg
      601 ccgacgcgct gtcgagaagc gccgcccgag tcggtccctc ggacggggcg cccttctggt
      661 agcactccgc gatgagggac aggtactgca ccgtcccggc cgcgtcgtac gccatcgcgg
      721 cgcgatacgc cgggatggct tccatgaagc ggccgctccc gtagagccgg ttcccgcgcc
      781 ccatcgcagc gacgcgcagg ttgcgcgtga gctgctcgtc gccgggtttg agctcgaggc
      841 cccgctgcgc cgccttgatc gcgtcgtccg ggcggccgag aaggtcgtac gtgtagctca
      901 aattcttgta ggtatcgggc gacgagggat cgatcagcat cgcgaggccg aagtcgtagg
      961 aggcctccgc gaccgccgag cctcccgtgt acgccgggaa cacggtcgtg tcggtgaccg
     1021 cgacgctcgg atcggccgag accgaaaacg gcttgtacgg gatctggatc ggatccgccc
     1081 cctgaggaaa gtagtagacg gtggactgat ccgggtaata ccagaactcc tgcatcttcc
     1141 cgttctcggt gaagcggctg aagtccctgg gctccccgcg ctccttcgcg accgcggcct
     1201 gcggcgtccc ttcgtcggtc gtgcgcgcgt cgtcctgcgc gatcttgccc gcgcggccca
     1261 gcgccttgat gccctggttg aagaggtcgg cgtagagcgc cttccgattc tgctcgatct
     1321 cgtcggcgcg cttcttgtcc ttcttgtcgg tgcagacctt gatgccgatc tggaatgcgc
     1381 cgccggcgga cgcgaactga cgaagctgcg acctcgagaa accgaggagc gaatagacct
     1441 gcgtgttgtc cggctcctcc ttgcgcgcaa gctcgaggta gtgggccgcc ttgtcgtaga
     1501 ccttctgctg aacgtagacc ttgccgctcg tgaagttgac gccggccgag gccggctggg
     1561 ccacgaggag cgagacgagg ccgatggcca cgagcgcaca aacgatcccg cgagcgggga
     1621 tgaaaccctt catgaatgcc tccatgaaac tatgtacccg gtgagggggc gacgcgttcc
     1681 agcggcccat tttctcgtgc gattcaagca caggggagcg gcctgtcaag gggttgcaat
     1741 tacagaagct gcctcgggtc cacgtccacg gccacgtgca cgcccggaag cttgagcccc
     1801 cggatcgccg gaattccccg tttcagatgc tcccgcaagg actttcggga ggggcttcta
     1861 aggaggaggt gccatcgttc cttgccttgg atgcgggaaa tcggctttgg tgccggcccc
     1921 aggatctcga ccgccggctg gatccgggcc agagccttcc gaactcgttg cgccgcctcg
     1981 accacccggt cgagggtggc gcctcggatc tcgaccgcga ccaggtgccc gtagggggga
     2041 tagccgagct cccggcgctc cgcggcttcg agcgcgaaga aggcctcgaa gtcctgatcc
     2101 gcggcgcagc gaagcgcggg atggtccggg ttgtaggact gcatgatcac ttcgcccgga
     2161 ctctttcccc ggcccgcgcg cccggcgacc tgggtgagga gctggaacgt ccgctccgcc
     2221 gcgcggaagt cgggcaggaa gagctggaga tccgcgacga tgatgcccac cagcgtgacg
     2281 ccggggaaat cgaatccctt cgtcaccatc tgcgtgccga gcaggaagtc cgtgtctccc
     2341 tgacggaacg atctgaggat cccgcccgcc gcgccccgct ttcgcacggc gtcgaaatcg
     2401 aggcgcgcga gccgcgcgtg cgggaacagg cgcgccactt cgcgctcgat cttctggatc
     2461 ccgacgcccc cgagccagag atcggacgcg ccgcactcct tgcacgacag gaggagccgg
     2521 cgcgtggcgc cgcagtagtg acagcggagc gtctggtcct cggtgtgcac cgtgagcgac
     2581 acgtcgcacc gcgcgcacga cgcgacggag ccgcagcctc ggcactggag cacggtcgag
     2641 tggccgcgcc ggttgagaaa gaggatcccc tgctcgcccc ggaccagcgt tcgctccatg
     2701 gcggtgagga gcgcgggcgt gaggtacgcc gatgagggct gcacgggggg cgcttccgcg
//
LOCUS       DASTKC010000026         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_20917, whole genome shotgun sequence.
ACCESSION   DASTKC010000026 DASTKC010000000
VERSION     DASTKC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20917"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFR25_00920"
     CDS             complement(<1..894)
                     /locus_tag="VFR25_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9939641.1"
                     /translation="MPPLILLTALLEGLSLTLIQGYLPLYLRKALGEPSYLTVALVVA
                     LPALGAMIASNVWGGLADVSGRLKPFILLGLTGYVIALLGIPAFHTGFSVLLFVGMAS
                     LLYGTTAPSIKTYATLVKPDRKEFALASVLMAQSTGWLLGSYGAGGLLERGIGSGLRL
                     ALWSCAGLMALHLIVSTIALRDVRREPVAPRSRESWAARIAADLRSLYENPRLFRLCT
                     LAFFFVAANYIVWGFFSVFMVEHLHSSIHTLRYSLAASSVLGIISFLYVGPVIRRFHG
                     RLVLAIGITLYMGMYLGMALAR"
     gene            1058..2245
                     /locus_tag="VFR25_00925"
     CDS             1058..2245
                     /locus_tag="VFR25_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HET9939642.1"
                     /translation="MSIPLRSRLSSAFALVAALSVSLLTLAGCGNAPSAKTEAKNAVA
                     TSAPPERAINFKIGIMTGTVSQGEDEFRAGQMVIKKYGADHVRHVTYPDNFMNEQETV
                     ISQLVGLADDPDVKVIIVGQAIPGSIAALRKIKEKRPDIKMAFIEAHEDPSMVNEAAD
                     LSIQPDQLARGRTIVEVAKSMGVTDLVHYSFPRHMSQYLLAKRRDIMKEECAKNGIKF
                     HFVTAPDPMGEGGLNATQQFVVEDVPRELKQYGPKTAFFSTNCGMMDPMIKTVVQNGG
                     YIPEQCCPSPTHGYPTALGIAIPADKAGDMAYINAENKKVIADHHMTGHFATWPVPEV
                     MLSIRAMTDLMVDSYEGKADYKNPATVKQYMEQEAGVPITIAKYDPAKGNSYLFLVSN
                     IYY"
     gene            2274..>3484
                     /locus_tag="VFR25_00930"
     CDS             2274..>3484
                     /locus_tag="VFR25_00930"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9939643.1"
                     /translation="MGTTALELRGITKEYEGVRVVKGVSLAVRAGEIHALLGENGAGK
                     STLMNILFGMPVIRETGGYTGEILLDGRPIRFGSPGEAIEAGIGMVHQEFMLLPGFRI
                     AENIKLNREPTRRSLLSRVFGRGLERLDEGRMREDARRALDRVGLTIYEWLPVAGLPV
                     GHMQFVEIAREVDKDRLRVLIFDEPTAVLTESEAERLLATMREFARAGVAVLFITHRL
                     EEVRAVADRATVLRDGEVVGVVDPKTTTVVRIAEMMVGRPAAEVAGRRVFPAGAEAAL
                     TIEHLRVDMPGELVEDISLVVRRGEILGIGGLAGQGKIGIANGVMGLYPANGRVVFEG
                     RELALNRPREALERGLAFVSEDRRGVGILPDETIELNVALSAIEAQDRFMLPGPIGPL
                     RLLDGAAVRRHA"
BASE COUNT          626 a         1115 c         1218 g          525 t
ORIGIN      
        1 ccgcgcgagc gccatgccca gatacatgcc catgtagagc gtgatgccga tggcgagcac
       61 gagtctcccg tgaaagcggc ggatcacggg accgacgtac aggaacgaga tgatcccgag
      121 cacgctcgac gcggcgagcg aatagcggag cgtgtggatg ctcgagtgga ggtgctccac
      181 catgaagacc gagaagaagc cccacacgat gtagtttgcc gccacgaaga agaacgcgag
      241 cgtgcagagg cggaagagcc tcggattctc gtagagggag cggaggtcgg cggcgatgcg
      301 cgcggcccag ctctcgcggg agcgcggtgc gacgggctcg cgtcggacat cccgaagcgc
      361 aatcgtcgac acgatgagat ggagcgccat cagcccggcg cacgaccaga gcgcgagcct
      421 caaccccgaa ccgatcccgc gctcgagcag gcctcccgcg ccatagctcc cgaggagcca
      481 ccccgtcgat tgcgccatca gcacggacgc gagcgcgaac tccttgcggt ccggtttgac
      541 cagggtggcg taggtcttga tcgacggcgc cgtggtcccg tagaggagcg acgccattcc
      601 cacgaagagg aggacggaga aaccggtgtg gaaggcgggt atacccagga gggcgatcac
      661 gtagccggtc agacccaaga gaatgaacgg tttcagtcgc cccgagacgt cggcgagtcc
      721 cccccagaca ttgctcgcga tcatggctcc gagcgcgggg agcgccacga cgagcgcgac
      781 cgtgaggtac gacggttccc cgagggcctt gcggagatag agcggcagat agccctggat
      841 gagggtgagc gaaagcccct ccaggagcgc ggtgagcaga atcaggggcg gcatggtcgg
      901 gcgaggctag cacggtaggc ggcgtggagt gacggcggct gggccgggcc cgcgtcgctg
      961 gtgcggcctc gctcgtaagt gctaaactgg gccgatcctc gttccggttc ctcgccggcc
     1021 cctacggccc gcctcggcgg gcggaggcaa tccacccatg tcgatcccgc ttcgctcgcg
     1081 cttgtcctcg gccttcgccc tggtcgcggc gctttcggtt tcgctcctca cgctcgccgg
     1141 ctgcggcaat gcgccttccg ccaagaccga ggccaagaac gcggtcgcca cgtcggcgcc
     1201 gcccgagcgc gcgatcaact tcaagatcgg catcatgacc ggcaccgtct cccagggtga
     1261 ggacgagttc cgggccgggc agatggtcat caagaagtac ggcgcggatc acgtgcgcca
     1321 cgtgacctat cccgacaact tcatgaacga gcaggagacc gtcatctcgc agctcgtcgg
     1381 tctcgcggac gatccggacg tgaaggtgat catcgtgggc caggcgatcc cgggctccat
     1441 cgcggcgctc cgcaagatca aggagaagcg ccccgacatc aagatggcct tcatcgaggc
     1501 gcacgaagat ccgtcgatgg tgaacgaggc cgccgatctc tccatccagc ccgaccagct
     1561 cgcgcgcggg cggaccatcg tggaggtcgc gaagtcgatg ggcgtgaccg atctcgtcca
     1621 ctactccttc ccgcggcaca tgtcgcagta cctcctcgcc aagcggcgcg acatcatgaa
     1681 ggaggagtgc gcgaagaacg ggatcaagtt ccacttcgtg accgccccgg atcccatggg
     1741 cgagggcggg ctcaacgcga cgcagcagtt cgtggtcgag gacgtgcctc gggagctcaa
     1801 gcagtacggg ccgaagacgg cgttcttctc caccaattgc ggcatgatgg atccgatgat
     1861 caagacggtc gtccagaacg gcggctacat ccccgagcag tgctgcccca gcccgacgca
     1921 cggttatccc accgcgctcg gcatcgcgat cccggcggac aaagccggag acatggccta
     1981 catcaacgcc gagaacaaga aagtcatcgc cgaccaccac atgaccggcc atttcgcaac
     2041 gtggcccgtg cccgaggtga tgctctccat ccgcgcgatg accgacctca tggtcgattc
     2101 ctacgagggc aaggccgact acaagaaccc ggcgacggtg aagcagtaca tggaacagga
     2161 ggccggcgtc ccgatcacga tcgcgaagta cgatccggcc aagggcaact cctacctgtt
     2221 ccttgtcagc aacatctact actagctccg acggcggggg cgaccccgcc gctttgggca
     2281 cgaccgccct cgagctccga ggcatcacca aggagtacga gggcgttcgc gttgtgaagg
     2341 gcgtctcgct cgcggttcgc gcgggcgaga tccacgcgct cctgggggag aacggcgcgg
     2401 gcaaatccac gctgatgaac atcctcttcg ggatgccggt catccgggag acgggcggct
     2461 acacgggcga gatcctgctc gatggccgcc cgattcgttt cggatcgcct ggcgaggcga
     2521 tcgaggcggg gatcgggatg gtgcatcagg aattcatgct gcttcccgga ttccggatcg
     2581 ccgagaacat caagctgaac cgggagccga cgcggcggtc gctcttgtcg cgggtgttcg
     2641 ggcgcggatt ggagcggctg gacgaggggc gcatgcgcga ggacgcgcgg cgtgccttgg
     2701 atcgagttgg cttgaccatc tacgagtggc tgcccgtcgc ggggctgccc gtgggccaca
     2761 tgcagttcgt cgagatcgcg cgcgaggtgg acaaggatcg gttgcgggtc ctcatcttcg
     2821 acgagccgac ggccgtgctc acggaatccg aagcggagcg actcctcgcg acgatgcggg
     2881 agttcgcgcg cgcaggcgtg gccgtcctct tcatcacgca ccgactcgag gaggtgcgcg
     2941 cggtcgccga ccgtgcgacc gtgctgcgcg acggcgaagt ggtgggcgtc gtggacccca
     3001 agaccaccac ggtcgtgcgg atcgcggaga tgatggtcgg gcggccggcg gcggaggtgg
     3061 cgggccggcg cgtgttccct gccggcgccg aagccgcgtt gacgatcgag cacctgcgcg
     3121 tcgacatgcc cggcgagctc gtcgaggaca tctctctggt cgtgcgccgt ggcgaaatcc
     3181 tcgggatcgg cggtctggcc ggtcagggga agattgggat cgcgaacggc gtgatggggc
     3241 tctatccggc gaacgggcgc gtggtgttcg agggacgcga gctcgcgttg aatcgcccgc
     3301 gcgaggcgct ggaacgcggt ctcgcgttcg tctccgagga ccggcgcggc gtcggcatcc
     3361 tgcccgacga gaccatcgag ctgaacgtgg cgctgtcggc gatcgaggcg caggatcgct
     3421 tcatgctgcc gggcccgatc ggcccgctgc ggctgctcga cggcgccgcg gtgcgccgcc
     3481 acgc
//
LOCUS       DASTKC010000027        10381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_731329, whole genome shotgun sequence.
ACCESSION   DASTKC010000027 DASTKC010000000
VERSION     DASTKC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731329"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFR25_00935"
     CDS             <1..266
                     /locus_tag="VFR25_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET9939644.1"
                     /translation="SLSPNPLNPGGILRLTTTRDGFVRVRMFDLQGRMVRVLEDRAMV
                     PAGAHDVRIDGRNTSGQTLASGIYFYQVDTAEGSLKGRITVLK"
     gene            complement(381..1841)
                     /locus_tag="VFR25_00940"
     CDS             complement(381..1841)
                     /locus_tag="VFR25_00940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9939645.1"
                     /translation="MKMPFGIDAPLGMIEPLIIVSVLGILLLLVDLLLPHGKKHHTAW
                     LALAGIVYAMIRTYSQLGQFQVGYSGMVVLDSLSTVLNLLFLASTGVVILLSMPFLKK
                     EDVEPSEYYALLLFATAGMMILGSGLDLITLFLGIEVLSISLYILAGYRRDSDASNEA
                     AMKYFLLGAFASSVLLYGIALVYGATGTTSLVRIAETVSGSATMPEGLMFAGVALILV
                     GLAFKVAAAPFHMWTPDVYEGAPTPITAFLSAGPKAAAFAALIRTFFIGFGPIQAEWN
                     TILAVIAALTMTIGNIAAIAQTRVKRMLAYSSIAHSGYVLIGIVAGGAAGGAAAVFYL
                     FAYVAMNLGAFAVIVLLEHKGERGEELRDYAGIGFRYPVIGALLSLFMISLSGIPPTA
                     GFMGKLYLFGAAVQSGHVLLAVIGVLNSAISVFYYLRLMVLMYMREEQETLPAIRVPL
                     TLAICLLVAAVATLWPGLWPSAWIGAAREGMRALFS"
     gene            complement(1838..3478)
                     /locus_tag="VFR25_00945"
     CDS             complement(1838..3478)
                     /locus_tag="VFR25_00945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9939646.1"
                     /translation="MSGTLTILGMPILSLLIFLPLVGTALVLMIPSSKPGAVRGTAFL
                     VSVLTFVLSIPLITMLDGARSGMQFVERKEWVPNLGISYFLGVDGISALLIVMTTFLS
                     AIAILSSFGAITNRVKTYYATLLALETGMIGVFAALDLVLFYVFWEAVLIPMYLIIGV
                     WGGPRRVYAAVKFILYTVAGSLLMLVAILYLYFSFHAATGEFTFDLLRLYNTPLSHPA
                     QLWLFGAFALAFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFVRFAMP
                     LFPDAAHELAPLMILLAVIGIIYGALVAMVQPDMKKLVAYSSVSHLGFVMLGLFAMNT
                     QGLQGGVIQMINHGLSTGALFLAVGIIYERRHTREISEFGGIAEILPWFAAFFLIICL
                     SSLGLPGLNGFIGEFLILLGAFRVHPKVAAIAATGVILAAVYLLWMYQRVMFGPVTNE
                     KNRGLRDLSPREFWTLAPVLLMILWLGVYPNPFLRKLDGSVADLVARMGRTMAQVDKP
                     GQALPASAAGGAEPAADAAVPAVDAAGPAAVPAEGGSR"
     gene            complement(3475..5409)
                     /gene="nuoL"
                     /locus_tag="VFR25_00950"
     CDS             complement(3475..5409)
                     /gene="nuoL"
                     /locus_tag="VFR25_00950"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9939647.1"
                     /translation="MLDYLGWIPALPLIGAIVNGLGAGRLPRKAVSAIGTGTVGLAFV
                     IALGCFQALLQFPPDQRYHLEQAFSWVDSGDLSVPVRLLLDPLSMVMVLIVTGVGFLI
                     HVYSTGYMGHEKAYGRYFAYLNLFTFSMLVLVMADNFLLMFFGWEGVGLCSYLLIGYW
                     YQRPSAASAGKKAFVVNRIGDWGFMIGMFLIFVSFTTLDFGRVFAEAPSRLMPGSTMA
                     TAIALFLFIGATGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMIARCHILY
                     TLAPAALMVVAVVGAATALFAATIGLLQKDIKRVLAYSTVSQLGYMFLAMGVGAFASG
                     IFHLMTHAFFKALLFLGSGSVIHALSGEQDMDRMGGLKSALPKTHVTMLIATLAIAGI
                     FPFAGFFSKDEILFHAWESGGPILWAVGVIGAFLTAFYMFRLYFMTFHGPSRLTEEAK
                     HHLHESPNSMTIPLMILAVLSVVGGFIQIPLVEGGQRLSEFLAPVFADVTHVAGGAAA
                     AHGAEHGGSGLEIALMVISLAVAAAGIFLAYRFYMVDPAAPKRLAEQMRGLYRLVFNK
                     YWVDEVYNDKIVEPIRRGSIALWQKFDAAVIDGAVNGVGRQIERGAALLRQAQTGSVQ
                     VYAMVITLGLVVVFGYLALR"
     gene            complement(5412..5729)
                     /gene="nuoK"
                     /locus_tag="VFR25_00955"
     CDS             complement(5412..5729)
                     /gene="nuoK"
                     /locus_tag="VFR25_00955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9939648.1"
                     /translation="MTPAPIIPLVYYQVLAAIIFTIGVVGVLVRRSALIIFMSIELML
                     NAVNLSFVAFSRQHGSMDGHIFVFFVMAVAAAEVAVGLAILVAIFRNRDTANVDDVRL
                     LRG"
     gene            complement(5726..6241)
                     /locus_tag="VFR25_00960"
     CDS             complement(5726..6241)
                     /locus_tag="VFR25_00960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9939649.1"
                     /translation="MLISAFLFAMFAGIAIGCSLGVIFHRNPVHCALMLVGVLLSVSG
                     LFILLNASFLAALQVLVYAGAIMVLFLFVLMLLNLKGENPLLTRGAAKGFGILFAIVV
                     FVELLWTVLSPQVEAGSMGAVPTVPRGFGSPAAIGRVLYTVWLYPFEITSILLLIAVI
                     GAVVLAKRKFG"
     gene            complement(6249..7229)
                     /gene="nuoH"
                     /locus_tag="VFR25_00965"
     CDS             complement(6249..7229)
                     /gene="nuoH"
                     /locus_tag="VFR25_00965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET9939650.1"
                     /translation="MDTAFLIESVIKVHIVLGFVLLVVPFIVWMERKVLGHMQDRIGP
                     ERVGPFGLLQTIADGIKLFMKEDLVPAQADKAVFILAPGITVVTALIATTVIPWGDTV
                     TLFGRTIPLHIADVNVALLFVLATTSMGVYGVVLGGWASNSKYPLLGGLRSSAQMISY
                     ELAQGISLVSVVLLAGTLSLAQIARQQAHMWYVVPQFFGFAIYVISGIAETNRAPFDL
                     PEAETELVAGFHTEYSSMKWAFYFLGEYANMMVVSGVAITVFLGGWNGPVLPGPVWFL
                     IKLTAFLFFFIWLRATFPRLRYDQLMAFGWKVLLPASILNLVVTAIVVAL"
     gene            complement(7233..7700)
                     /gene="nuoE"
                     /locus_tag="VFR25_00970"
     CDS             complement(7233..7700)
                     /gene="nuoE"
                     /locus_tag="VFR25_00970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET9939651.1"
                     /translation="MALSDKSKKQIRELLKTYETNQSALIPALHVAQGDQGWLSEETQ
                     REVAELLEISSQEVRGVVSFYTMFLQKPAGKYLLQVCRNLSCSLRGGHKLTKQLEDKL
                     QIKQGETTTDGKFTLIEVECLGSCGTAPVMMVNDKYVEDLTPQALDKMIAELT"
     gene            complement(7700..8224)
                     /locus_tag="VFR25_00975"
     CDS             complement(7700..8224)
                     /locus_tag="VFR25_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9939652.1"
                     /translation="MTPSPEHLDPLSTDQQKVVERFRSTLGDAVLRERSFRGQLSIWI
                     KVESILEALKLAKTDPALDCALLTDLTALDMLWRQEPGEPRFEVVYNLYSFRHNRRFL
                     LKTGVEEGQTVPTATTLWPGANYMEREVWDLFGIRFEGHPNLERILTPDGWLGHPLRK
                     DFPTMSDQFPNVEN"
     gene            complement(8221..8550)
                     /locus_tag="VFR25_00980"
     CDS             complement(8221..8550)
                     /locus_tag="VFR25_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9939653.1"
                     /translation="MMVVVATGFAVVTIAASYYLGRRVNTAAKLMPYECGITPVGNAR
                     DRFHTRFYLVAMLFIVFDIETVFLYPWAVVYRQLALFGLIEMAVFIAILLAGLVYVWG
                     KGALEWD"
     gene            complement(8717..9577)
                     /locus_tag="VFR25_00985"
     CDS             complement(8717..9577)
                     /locus_tag="VFR25_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9939654.1"
                     /translation="MEASTIPSLTIGPYKVHFVAGGRFRLDGGAMFGVVPKVLWNRAM
                     PADERNRIQMRMTCLLLEGNGQKILVDAGSGTKDDPKFRDIYAIEGPEWLTQELAALG
                     VKPEDVNAVAMTHLHFDHCGGGTTRGADGKAKPTFPNAHYFVRRQEWEDAHHANERNR
                     ASYISDNYDPLHDAGQLILHDNDLEILPGVWMKNLPGHTLGHQGCFFDIPGARALYTV
                     DLIPTVSHLPLAYIMGYDLYPMMTLETKRAILRQATAEGWLFLFEHDPELCAIRVGGS
                     EQKVTFEKVA"
     gene            complement(9587..9952)
                     /gene="rnpB"
                     /locus_tag="VFR25_00990"
     ncRNA           complement(9587..9952)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFR25_00990"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         2033 a         3354 c         3396 g         1598 t
ORIGIN      
        1 cttcgctctc ccccaatccg ctcaacccgg gcggcattct gcggctcaca accacgcgcg
       61 acggattcgt tcgcgtgcgg atgttcgatc tccagggccg gatggtgcgc gtgctcgagg
      121 atcgtgccat ggtgcccgcg ggagcgcatg acgtgcggat cgacggacgg aatacttccg
      181 gccagacgct cgcgtcgggg atctacttct accaggtgga tacggcggag ggatcgttga
      241 agggtcggat cacggtcctc aagtagccaa ggtccaagcc acaaacaaga gagggcggcc
      301 cgtcctgggt cgccctttca cttgccccgg gacaagttgc ggtgcctgct ccattcaact
      361 tgcgacgtcg caagctggtg ctacgagaag agcgcccgca ttccttcccg cgcggcgccg
      421 atccacgcgg agggccagag gcccggccaa agtgtggcca ccgccgcgac caagaggcag
      481 atcgccagcg tcaacggaac ccggatcgcg ggcagcgtct cctgctcttc cctcatgtac
      541 atgagcacca tgagccgcag gtagtagaag accgagatcg cgctgttcag cacgccgatc
      601 accgccagga gcacgtgccc cgattgcacg gccgcgccga agaggtagag cttccccatg
      661 aagcccgccg tcggcgggat ccccgagagc gagatcatga agagcgacag gagggcgccg
      721 atgacgggat accggaaccc gatcccggcg tagtcgcgga gctcttcccc gcgctcccct
      781 ttgtgctcga ggagcacgat caccgcgaac gctccgagat tcatcgccac gtacgcgaac
      841 agatagaaca ccgccgcggc ccctccggct gctcccccag ccacgatccc gatcagcacg
      901 tatccggagt gcgcgatgct cgagtaggcg agcattcgct tcacgcgggt ctgcgcgatg
      961 gccgcgatat tcccgatggt catggtcaaa gccgcgatca cggcgagaat cgtgttccac
     1021 tccgcctgga tcgggccgaa gccgatgaag aaggtccgaa tgagcgccgc gaacgccgct
     1081 gccttgggac cggcggacag gaacgccgtg atcggcgtcg gcgctccttc gtagacgtcg
     1141 ggcgtccaca tgtggaacgg ggcggccgcc accttgaagg cgaggccgac aaggatcagc
     1201 gcgactcccg cgaacatgag cccctcgggc atcgtggccg agccgctcac cgtctcggcg
     1261 atccggacca ggctcgtcgt cccggtggcg ccgtagacga gcgcgatgcc gtagaggagg
     1321 acggacgacg cgaaggcgcc gagcaggaag tacttcatgg ccgcctcgtt cgacgcgtcg
     1381 gaatcgcggc gatagccggc caggatgtag agggagatcg agagcacctc gatccccagg
     1441 aacagcgtga tcagatcgag gcccgagccg aggatcatca tcccggcggt cgcgaagaga
     1501 aggagcgcgt agtactccga cggctccacg tcctccttct tcaagaacgg catggagagg
     1561 aggatcacga cccccgtgct cgccaggaag agcaggttga gcacagtcga aagactgtcc
     1621 agcaccacca tgcccgagta gccgacctgg aactggccta actgcgagta ggtccggatc
     1681 atcgcgtaga cgatccccgc gagcgcgagc cacgcggtgt ggtgcttctt cccgtgcggc
     1741 aggaggagat cgacgaggag gagcaggatc ccgaggaccg agacgatgat gagcggctcg
     1801 atcatgccga gcggggcgtc gataccgaag ggcatcttca tcgggagcct ccctcggcag
     1861 gcacggcggc aggcccggca gcgtcgacgg ccggcacggc tgcgtcggcg gccggttcag
     1921 ctccacccgc ggcagacgcc gggagcgcct gccccggctt gtccacctgc gccatcgtgc
     1981 gccccatccg cgcgaccagg tccgccaccg atccgtcgag cttgcgcagg aacgggttcg
     2041 ggtagacgcc gagccagagg atcatcaaca gcaccggcgc cagcgtccag aactcgcgcg
     2101 gcgagaggtc gcggagcccg cggttcttct cgttcgtcac agggccgaac atgacccgct
     2161 ggtacatcca gaggaggtac acggccgcga ggatcacccc ggtggcggcg atcgcggcca
     2221 ccttcggatg cacgcggaac gcgccgagca ggatcaggaa ttcgccgatg aacccgttca
     2281 gccccggcag tcccaggctc gagaggcaga tgatcaagaa aaaggccgcg aaccagggca
     2341 ggatctccgc gatgccgccg aactccgata tctcgcgcgt gtgccttcgc tcgtagatga
     2401 tcccgaccgc gaggaacaga gcccccgtcg agagcccgtg gttgatcatc tggatcacgc
     2461 ccccctggag cccctgcgtg ttcatcgcga agagcccgag catgacgaag ccgaggtggc
     2521 tgaccgagga gtaggccacg agcttcttca tgtccggctg caccatcgcg acgagcgccc
     2581 cgtagatgat cccgatcacg gcgaggagga tcatcaaggg ggccagctcg tgcgccgcgt
     2641 ccgggaagag cggcatcgcg aagcggacga agccgtacgt ccccatcttg aggagcacgc
     2701 ccgcgaggat caccgagccc gcggtcggcg cctccacgtg cgcgtcgggc agccacgtgt
     2761 ggaacgggaa catcggcacc ttgatcgcaa aggcgagcgc gaaggcgccg aacagccaga
     2821 gctgcgccgg atgggagagc ggcgtgttgt agagtcgcaa gaggtcgaac gtgaactcgc
     2881 cggtcgcggc gtggaacgag aagtacaggt agaggatcgc caccagcatg aggaggctcc
     2941 cggccaccgt gtagaggatg aacttgacgg ccgcgtagac gcgccgggga ccgccccaga
     3001 ctccgatgat caggtacatc gggatcagga ccgcctccca gaagacatag aagaggacca
     3061 ggtccagcgc ggcgaagacg ccgatcattc ccgtttcgag ggcgaggagc gtcgcgtagt
     3121 aggtcttcac ccggttcgtg atcgcgccga acgacgaaag gatcgcgatc gccgacagga
     3181 acgtcgtcat cacgatcagg agcgcgctga tcccgtccac gccgaggaag taggagatgc
     3241 cgagattcgg cacccactcc ttacgctcca cgaattgcat ccccgagcgg gctccatcga
     3301 gcatggtgat caaagggatc gagagcacga acgtcagcac cgacacgagg aacgccgtcc
     3361 cgcgcaccgc gcccggcttc gaggacggga tcatgaggac gagcgccgtc cccacgagcg
     3421 gcaggaagat gaggagcgaa aggatcggca tgccgaggat ggtcaaggtc ccgctcatcg
     3481 aagcgccagg tagccgaaca cgaccaccag ccccagcgtg atgaccatgg cgtagacctg
     3541 caccgagccg gtctgcgcct gccggaggag tgcggctccg cgctcgatct gccgtcccac
     3601 cccgttcacc gcgccgtcga tgacggcggc atcgaacttc tgccagagcg cgatcgagcc
     3661 ccgccggatc ggctccacga tcttgtcgtt gtagacctcg tccacccagt acttgttgaa
     3721 gacgagccgg tagagcccgc gcatctgctc cgcgaggcgc ttgggcgctg ccggatcgac
     3781 catgtagaag cgatacgcga ggaagatccc cgccgccgcc accgcgagcg agatcaccat
     3841 gagcgcgatc tccagaccgg aacccccgtg ctcggcgccg tgcgccgccg cggccccgcc
     3901 ggccacatgc gtcacgtccg caaagacagg agcgaggaac tccgaaagcc tctgcccacc
     3961 ttccaccagc gggatctgga tgaacccgcc caccacgctc agcaccgcga ggatcatcag
     4021 cgggatcgtc atgctgttcg gcgactcgtg gaggtgatgc ttggcttcct ccgtcaggcg
     4081 cgacgggccg tggaaggtca tgaagtagag ccggaacatg tagaacgccg tcaggaacgc
     4141 tccgatcacg cccaccgccc agagaatggg tccgccagac tcccatgcat ggaacaggat
     4201 ctcgtccttc gagaagaagc ccgcgaacgg gaagattccg gcgatcgcga gcgtcgcgat
     4261 cagcattgtc acgtgcgtct tcgggagcgc cgacttgagg ccgcccatcc ggtccatgtc
     4321 ctgctcgccg gagagcgcat ggatcacgct ccctgacccg aggaagagga gcgccttgaa
     4381 aaaggcgtgg gtcatgagat ggaagatgcc cgacgcgaac gcgccgacgc ccatggcgag
     4441 gaacatgtag ccgagctggc tcacggtgga gtaggcgagc acccgcttga tgtccttctg
     4501 aaggagcccg attgtcgccg cgaagagcgc cgtcgctgcg cccaccaccg cgacgaccat
     4561 gagcgcggcc ggcgcaaggg tgtagaggat gtggcagcgg gcgatcatgt agacgccggc
     4621 cgtcaccatc gtcgcggcat ggatcagggc gctcaccggc gtcggacctt ccatggcgtc
     4681 cggaagccag acgtagagcg ggatctgggc cgacttgccg gtcgcgccga tgaagaggaa
     4741 gagtgcgatc gcggtcgcca tggtgcttcc cggcatgagc cggctcggcg cctcggcgaa
     4801 gacccggccg aagtcgagcg tcgtgaacga gacgaagatc aggaacatcc cgatcatgaa
     4861 gccccagtcg ccaatccggt tcacgacgaa ggccttcttc cccgccgatg ccgccgaggg
     4921 gcgctggtac cagtagccga tcaggaggta cgagcagagc ccgacccctt cccacccgaa
     4981 gaacatgagg aggaagttgt cggccatcac gagcacgagc atcgagaagg tgaacagatt
     5041 caggtacgcg aagtagcggc cgtacgcctt ctcgtgtccc atgtagcccg tcgagtagac
     5101 gtggatgagg aagcccacgc cggtgacgat caggaccatc accatcgaca gaggatccag
     5161 gagcaggcgc accgggaccg agagatcgcc cgagtcgacc cacgagaacg cctgctccag
     5221 gtggtagcgc tggtccggcg ggaactggag gagcgcctgg aagcagccga gggcgatcac
     5281 gaacgcgagc ccgaccgtac ccgtgccgat cgcgctcacc gccttgcgcg ggaggcgccc
     5341 ggcgccgagc ccgttcacga tggcgccgat cagcgggagc gccggaatcc agcccaggta
     5401 gtcgagcacc gttatcctcg caggaggcgg acgtcgtcga cgttcgccgt atcccggttc
     5461 cggaagatcg cgacgaggat ggcgagtccc acggccacct cggccgcggc caccgccatc
     5521 acgaagaaga cgaagatgtg gccgtccatc gagccgtgct ggcgtgagaa ggccacgaag
     5581 ctcaggttca ccgcgttcag catcagctca atcgacatga agatgatgag cgcgctccgc
     5641 cgcacgagca caccgaccac gccgatcgtg aagatgatcg cggccaggac ctggtagtag
     5701 acgagcggaa tgatgggagc cggggtcatc cgaatttcct cttggccagg accacggctc
     5761 cgatcaccgc gatgagcagc aggatcgacg tgatctcgaa gggatagagc cagaccgtgt
     5821 agagcacgcg cccgatcgcg gccggagagc caaagccccg cggcaccgtc ggcacggcgc
     5881 ccatgctgcc cgcctccacc tgcggcgaga gcaccgtcca gagcagctcc acgaaaacca
     5941 cgatcgcgaa caggatgccg aatcccttcg ccgcgcccct cgtgaggagc ggattctcac
     6001 ccttcagatt caggagcatg agcacgaaga ggaagagcac catgatcgcg cccgcgtaga
     6061 cgagcacctg gagcgccgcc aggaaggacg cgttcaagag gatgaagagc cccgagaccg
     6121 agaggagcac gcccacgagc atgagggcgc agtgcaccgg gttccggtgg aagatcaccc
     6181 cgagggagca gccgatcgcg atccccgcga acatggcgaa gagaaaggcg gagatcagca
     6241 tcgcgcgctc atagggccac cacgatcgcg gtcaccacga gattgaggat cgacgcgggc
     6301 aggagcacct tccagccgaa ggccatgagc tggtcgtatc ggagccgcgg gaacgtggcc
     6361 cggagccaga tgaagaagaa gaggaacgcc gtgagcttga tcaggaacca aacgggccct
     6421 gggagcaccg gtccgttcca ccctccgagg aacaccgtga tcgcgacccc cgagaccacc
     6481 atcatgttcg cgtactcgcc gaggaagtag aacgcccact tcatgctcga gtactcggtg
     6541 tggaacccgg ccacaagctc ggtctcggct tccgggagat cgaagggagc gcggttggtc
     6601 tccgcgatgc ccgagatcac gtagatcgcg aatccaaaga actggggaac gacgtaccac
     6661 atgtgggcct gctggcgcgc gatctgcgcg agactcagcg tccccgcgag gagcacgacc
     6721 gagaccagcg agatcccctg ggccagctcg taggagatca tctgcgccga ggacctgagc
     6781 cccccgagga gcgggtactt gctgttggac gcccagccgc cgagcaccac gccgtacacg
     6841 cccatcgagg tggtcgcgag cacgaagaga agcgccacgt tcacgtcggc gatgtggagc
     6901 gggatcgtgc ggccgaagag cgtcaccgtg tcgccccacg ggatcaccgt cgtcgcgatg
     6961 agcgcggtca cgaccgtgat ccccggcgcc aggatgaaca cggccttgtc cgcctgcgcc
     7021 ggcacgagat cttccttcat gaagagcttg atgccatccg cgatcgtctg gaggaggcca
     7081 aaggggccga cccgctcggg gccgatccgg tcctgcatgt gcccgagcac cttccgctcc
     7141 atccacacga tgaacggaac gacgaggagc acgaagccca ggacgatgtg gaccttgatc
     7201 accgactcga tgaggaacgc ggtatccacg cgctacgtga gctccgcgat catcttgtcg
     7261 agggcctgcg gggtgagatc ctccacgtac ttgtcgttca ccatcatcac gggagcggtg
     7321 ccgcaggagc cgaggcactc cacctcgatg agggtgaact tgccgtccgt cgtcgtctcc
     7381 ccctgcttga tctggagctt gtcctcgagc tgcttcgtga gcttgtgccc gccccgaagg
     7441 ctgcacgaga gattgcggca aacttgaagg aggtacttcc ccgccggctt ctgcaggaac
     7501 atcgtgtaga aggaaaccac gccgcgcacc tcctgcgacg aaatctcgag gagctccgca
     7561 acctcgcgct gcgtctcctc gctcagccag ccctggtccc cctgggccac gtggagcgcg
     7621 ggaatgagcg cggactggtt cgtctcgtac gtcttgagaa gctcgcggat ctgcttcttc
     7681 gatttgtccg agagcgccat cagttctcca cgttcgggaa ctggtcggac atcgtcggga
     7741 agtccttgcg cagcggatgc cccagccagc cgtcgggggt gaggatgcgc tcgagattcg
     7801 gatgtccctc gaaccggatc ccgaagagat cccagacctc acgctccatg tagttggcgc
     7861 cgggccagag ggtggtcgcc gtcgggacgg tctgcccttc ctccacgccg gtcttcaaga
     7921 gaaagcgccg gttgtggcgg aacgagtaga ggttgtagac gacctcgaac cgcggctcac
     7981 ccggctcctg ccgccacagc atgtccaggg cggtgagatc cgtgaggagc gcgcaatcga
     8041 gcgcggggtc ggtcttcgcg agcttcaagg cctcgaggat cgactccacc ttgatccaga
     8101 tggagagctg accccggaag gagcgctcgc ggagcacggc gtcgccgagc gtggagcgga
     8161 agcgctccac caccttctgc tggtcggtgc tgagcggatc gaggtgctcc ggacttgggg
     8221 tcaatcccac tcgagcgccc ctttccccca aacgtagacg agcccggcca ggaggatcgc
     8281 gatgaacacc gccatctcga tcaggccgaa cagggcgagc tgcctgtaga cgaccgccca
     8341 cgggtagagg aacacggtct cgatgtcgaa cacgatgaag agcatcgcca cgaggtagaa
     8401 gcgggtgtgg aaccgatcgc gggcattgcc gacgggggtg atgccgcact cgtacggcat
     8461 gagcttggcg gccgtgttga cccgccggcc cagataatag gaggcggcga tcgtcaccac
     8521 ggcgaagccc gtagcgacga cgaccatcat gaggatgggg atgaatgcgg ccgtgctgtt
     8581 cgggtcgacc ggactcacga gactccctgt cagggcaccc ctggacgggg ccttgccgag
     8641 ggctccaacg aaggcccgcg cgacatcgtg aaaaaaggtg ctaagcgcgg cagaatagag
     8701 acttggcccg tctccgtcaa gccactttct cgaaggtgac cttctgctcg cttcccccga
     8761 cgcggatggc gcagagctcg gggtcgtgct cgaagaggaa gagccagccc tcggcggtcg
     8821 cctgtcgcag gatggcccgc ttcgtctcga gcgtcatcat gggatagagg tcgtagccca
     8881 tgatgtaggc gagcggcaga tgggagacgg tcgggatgag atccaccgta tagagggcgc
     8941 gcgcgccggg gatgtcgaag aagcagccct ggtggccgag cgtgtgcccc ggcaggttct
     9001 tcatccacac gccgggcagg atctcgagat cgttgtcgtg caggatgagc tgccccgcgt
     9061 cgtggagcgg atcgtagttg tccgagatgt aggacgcgcg attccgctcg ttcgcgtgat
     9121 gcgcgtcctc ccactcctgc cgtcgcacga agtagtgcgc gttcgggaag gtcggcttgg
     9181 ccttgccgtc cgcgccgcgc gtggttcccc cgccgcagtg atcgaagtgg aggtgcgtca
     9241 tcgcgaccgc gttgacgtcc tcgggcttca cgccgagcgc ggccagctcc tgcgtgagcc
     9301 actcgggccc ctcgatggcg tagatgtccc ggaacttcgg gtcatccttc gtgcccgatc
     9361 ctgcgtcgac gaggatcttc tggccgttcc cctcgaggag cagacacgtc atccgcatct
     9421 ggattcggtt ccgctcgtcg gcgggcatgg cgcggttcca gaggaccttc ggcaccacgc
     9481 cgaacatcgc gccgccatcc aggcggaagc gtccgcccgc gacgaagtgg actttgtacg
     9541 gaccgatggt gagcgacgga atggtggatg cttccatggg acgatgtacc ggagtacctc
     9601 ataagccgag ttctgtcgga tcgctccgtg gcgaccattt ctctgggacg cgcgtctccg
     9661 cgcgcctcaa gcgacctacc cgggactcaa gcgaggcggg ccacctcatc gtcccctatt
     9721 cggtctttct ccgggtgggg tttacctagc cggaggcgtc gccgcctccg ctggtgcgct
     9781 cttaccgcac cgtttcaccc ttacctcagg cttgcgccca aggcggtctg ctctctgttg
     9841 cactttccct gggatcgctc ccgctgggcg ttacccagca ccctgcccta tggagctcgg
     9901 actttcctca ggccgcgcac gaagcgccgc ctgcggccgc ctgtggtact ccggcaatcg
     9961 gccgtgcctg cctgttacga tgcaacagct tacggggtga atcggatgga atcaaggggg
    10021 gcgccgttgt tcgaagtagc ccgggatggt tacaattcaa cggcgtcatc gccgtcgggc
    10081 tacaagcgag gtcatgccat gggctcccaa ctacgacatc gcgaccgact caggccctgg
    10141 ctcgttctag gatttgtcct catcctgctt cagacggttc tcccaggttg ctctgatgac
    10201 aatccaaccg cccccaaacc atcatccgga tcgtggcagg acattggacc gtcagccgaa
    10261 ttcgctgatg tgggagacct gaccacatgg aacgggcttc tggtcgccag cggcagcttt
    10321 catcagtttc aggggcacct cgattccaat ctccttacat gggatggagc gaactggcag
    10381 c
//
LOCUS       DASTKC010000028         6064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_259173, whole genome shotgun sequence.
ACCESSION   DASTKC010000028 DASTKC010000000
VERSION     DASTKC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259173"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFR25_00995"
     CDS             <1..606
                     /locus_tag="VFR25_00995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9939655.1"
                     /translation="LAAKGYEVTALDESFDAGAGADQVRWVERDLLHFDGEGAFDVVL
                     FTRSLHHMAPLDDALDRAIQALAPQGRVIAEEFAYDRVNLPTARWFYDLKTVLAAAGL
                     LQQSVGTEEDGNPLGRWRREHVADPPLPTGHSILAVARARLDLTLVEEAPYLYRYIWD
                     ELTNAEGARGAAEAVLRIERRLTRERDIAAAGLRFVGTPLG"
     gene            complement(634..1518)
                     /locus_tag="VFR25_01000"
     CDS             complement(634..1518)
                     /locus_tag="VFR25_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET9939656.1"
                     /translation="MSLRAAIVDAFTRTPGMGNRAGIVPDAARLDEPAMQRIAAAVAA
                     SETAFILEPPPGATHHMRYFTPAAEIAFCGHATVATFHWLVETGAVKPGQHVLHCPAG
                     RVPIEVEAMDGSGVRVWMGTPLYPWEPSPIAGATLMGLLGGSQAMRDSALPLERAGKN
                     VYVALSKRADLSSLQPRWDDLVAEGGRHGIGGFYVFTRDAAEPGSVTQGRYFAPAFGI
                     REDPVTGSASGPLAEYLARHGVIALGSGPVRVRAEQGDAMGKPGRVDLEVAGAAGKVE
                     SVRVGGAAVTVIEGSLKI"
     gene            1594..2406
                     /locus_tag="VFR25_01005"
     CDS             1594..2406
                     /locus_tag="VFR25_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HET9939657.1"
                     /translation="MIHGLHPVHLALLCLAAFCGGTVDAIAGGGGLITVPALLAAGLS
                     PHQALGTNKGQSSFGSFAAIFRYARERLVDWRLAAVAFPLGFLGSALGAILALHLKPG
                     VLRPIVLALLVVVGFVFASGRLRPKERIGPAPKLGFLVASFALAIGLYDGFFGPGAGT
                     FIIAGYAALLHLPLTRATANAKVLNFASNVAALLFFARGGMILWGLAIPMAAAQLLGG
                     YCGAHLAIRRGDALIRGVAVAVVLALVLWVALDVPVRIAPGSGTHSIPGGHR"
     gene            2403..2963
                     /locus_tag="VFR25_01010"
     CDS             2403..2963
                     /locus_tag="VFR25_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9939658.1"
                     /translation="MTCRPLLLGGLALMLVTAVSCNTPAPKQTEEAKPAAMTPLQRGE
                     YLATVGGCNDCHTPGTFYGAPDFKRQLAGSDLGWRGPWGVSYAGNLTPDLETGLGTWT
                     NVEIERALRSGVKKDGTPILPPMPWPNTARLTPDDMGSLIAYLKSLPPVVHKVPANLP
                     PNGMAHGPVIELPAPGAWDAPRTQPQ"
     gene            2960..3397
                     /locus_tag="VFR25_01015"
     CDS             2960..3397
                     /locus_tag="VFR25_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9939659.1"
                     /translation="MSPAATTALRPFHFAFHVLDLASTRRFYGDVLGCREGRSTETWV
                     DFDFFGNQISCHTTGPVAATAPTGKVENIHVPMPHFGAILGWDEFHALAERIRAAGFS
                     FVVEPRIRYAGQPGEQATMFLLDPSGNALEFKAFRHPEHVFTS"
     gene            3394..4551
                     /locus_tag="VFR25_01020"
     CDS             3394..4551
                     /locus_tag="VFR25_01020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9939660.1"
                     /translation="MTRASVAVIGAGVVGASVAYHLARRGWREVLLVDRAERLGLGST
                     GAATGGFRAQFASDANVRLSLLARERLSSFQQETGVDPGLVAAGYLWLARRAEDLEAL
                     RTALAIQRRSGFLDAREIGAREAVELNPAIDPDGVLGGTFAGSDGFLRPLAILEGYLR
                     AAERLGVTVRWNAEVTGLRRGHGGIEAVRVGADWILVEAVVNAAGAWAAPLAALADIT
                     VPIAPLRRQALASAPFAGLPETMPMTIWVDDGFHARVRDGRVLALMPTPGDPEDPTST
                     RVDPAWVASVTRIARERIPVLRDADFEHGTAWAGLYEMSPDGHALLGAAPGCANLFLA
                     NGSSGHGVMHAPALGLLAAEILSDGRASSMDTAALDPGRFAAGTVERRELL"
     gene            complement(4553..>6064)
                     /locus_tag="VFR25_01025"
     CDS             complement(4553..>6064)
                     /locus_tag="VFR25_01025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9939661.1"
                     /translation="EQDGHCNVMLADLKAKAGPIQLTDDPWYAFNPAFSPDGARIAWQ
                     EWDQLDMPWDQSRIVIAKLAKPIPACSTLFEAAPATVLSTIAKENASFAAPQWSPDGR
                     RFTYQSDVSGWRSLWIGDADGANAMPVDAGEGEIGLADWLPARSGYRWSDDGTAIYAV
                     RSRQSRDTLLRIDPSSRAVEDIPSAYTDIQGLQIRGHSLVYTATNPSTPQRLVTRIAQ
                     GARPGDEAARASSAVGLIDPESVSKPEVISWKTAGGAVSWGIYYPPVGPDAALGPRPL
                     IVLMHGGPTSQVPFSWQAQAQYYATRGWHYLIVNHRGGTGYGRAYQDQLRDSWGVVDR
                     EDARSGAEHVLKTRGADRSRVVITGGSAGGYATLWALTQEPDFWTAGIALFPLAHIYD
                     AVVGAHRFERHYEESLFGRLPKAGAVWMERSPITHVDRVKAPLLLFHGTADKAVPYQQ
                     SVDFADALRRRGGSVELVSYEGEGHGFAQEANRRDMMERMDRFLEKYVACLQR"
BASE COUNT          852 a         2155 c         2115 g          942 t
ORIGIN      
        1 ctcgccgcga agggctacga ggtgaccgcg ctcgacgaga gcttcgacgc gggcgccgga
       61 gccgaccagg ttcgctgggt cgagcgggac cttctccact tcgacgggga aggcgcgttc
      121 gacgtcgttc tcttcacgcg ctcgctccac cacatggcgc ccctcgacga cgcgctcgac
      181 cgcgcgatcc aggcgctggc gccgcagggg cgcgtgatcg ccgaggagtt cgcgtacgac
      241 cgcgtcaatc tcccaacggc gcgctggttc tacgatctca agacggtgct tgcggcggcg
      301 gggttgctgc agcaaagcgt ggggactgag gaggacggca atccgctcgg tcgttggcgc
      361 cgggagcacg tcgcggaccc gccgctcccg acgggacatt cgatcctcgc cgtcgcgcgt
      421 gcgcggctcg acctgacgct ggtcgaagag gctccctacc tctaccgcta catctgggac
      481 gagctgacga acgccgaggg ggcacgaggc gcggcggagg cagtgctccg aatcgagcgg
      541 cgcttgacca gggagcggga catcgctgcg gcgggtcttc gattcgtggg aacgccgctc
      601 ggctgagcgg cgcacatccc gcgcctgcgg gcgtcagatc ttgagcgagc cctcgatcac
      661 ggtaaccgcg gcgcccccga cgcggaccga ttccaccttc cccgccgcgc cggcgacctc
      721 gagatcgacc cgtcccggct tgcccatcgc gtccccctgc tcggcgcgaa cgcgcaccgg
      781 accgctcccg agcgcgatga cgccgtggcg cgcgaggtac tcggcgagcg gaccgctcgc
      841 ggagccggtc accggatcct cacgaatgcc gaacgcgggc gcgaaatagc gcccctgggt
      901 cacgctcccc ggctcggccg cgtcgcgcgt gaagacatag aagccgccga tcccgtggcg
      961 tcctccctcg gcgaccagat cgtcccagcg cggctgaagc gaggaaaggt cagcgcgctt
     1021 cgataaggcg acgtagacgt ttttccctgc acgctcgagc gggagcgccg agtcccgcat
     1081 cgcctggctt cctccgagga gccccatgag cgttgcgccg gcgatcgggg acggctccca
     1141 cgggtagagc ggcgtgccca tccaaacgcg aacgccgctt ccgtccatgg cctcgacctc
     1201 gatcggcacg cggccggcgg ggcagtggag cacgtgctgg ccaggcttca cggcgccggt
     1261 ctcgacgagc cagtggaacg tcgccacggt cgcgtggccg cagaacgcga tctccgcggc
     1321 aggcgtgaag tagcgcatgt ggtgagtcgc tccgggcggc ggctcgagga tgaacgccgt
     1381 ctcggacgcc gcgacggccg cggcgatccg ctgcatcgcg ggctcgtcaa ggcgggccgc
     1441 gtcggggacg atgcccgcgc ggttgcccat gcccggagtg cgtgtgaagg cgtcgacgat
     1501 ggcggcacgg aggctcacgg cgggagcata ccacgcgctt gcctcgagcc cgccggcgtg
     1561 cataatcgcg cgcatggcac gccgcgccgc gacatgatcc atggccttca ccccgtgcac
     1621 ctcgcgctcc tctgcctcgc ggccttctgc ggcggcaccg tcgacgccat cgccggcggc
     1681 ggcgggctca tcaccgtgcc cgcgctcctc gcggccggac tctccccgca ccaggcgctc
     1741 ggaacgaaca aggggcagtc gtcgttcggc tcgttcgcgg cgatcttccg ctacgcccgc
     1801 gagcgtctcg tcgactggag gctcgccgcg gtcgcgtttc cgctcgggtt cctcggctcc
     1861 gcgctcggag ccatcctcgc gctccatctc aagccaggcg tgctccgccc gatcgtgctc
     1921 gcgctcctcg tggtggtggg gttcgtcttc gcgagcggac ggcttcgccc caaggagcga
     1981 atcggtcccg cgccgaagct cgggttcctc gtggcctcgt tcgcgctcgc gatcggtctc
     2041 tacgacggct tcttcgggcc gggcgcggga acgttcatca tcgcaggcta cgcggcgctc
     2101 ctccatctcc cgctcacgcg cgcgacggcg aacgccaagg tgctcaactt cgcctcgaac
     2161 gtggccgcgc tcctcttctt cgcgcggggc ggcatgatcc tctggggcct tgcgattccg
     2221 atggcggcgg cccagctcct cgggggatat tgtggcgcgc acctcgccat tcgccgcggc
     2281 gacgcgctga tccggggcgt ggccgtggcg gtcgtgctcg ctctggtcct gtgggtcgcg
     2341 ctggacgttc cggttcgcat cgcgcccggc tcgggcacgc actccattcc aggaggacat
     2401 cgatgacctg tcgtccgctc ctgctcggcg gcctcgcgct catgctcgtc acggccgtct
     2461 cctgcaacac cccggccccg aagcagaccg aggaggcgaa gccggccgcg atgaccccgc
     2521 tccagcgcgg ggagtacctc gccacggtcg gggggtgcaa cgactgccat acgccgggca
     2581 cgttctatgg cgcgcccgac ttcaagcggc agctcgcggg gagcgatctc ggctggcgcg
     2641 ggccatgggg cgtgtcgtac gcgggcaatc tgacgcccga cctcgagacg gggctcggca
     2701 cctggaccaa cgtggagatc gagcgcgcgc tgcgctccgg agtgaagaag gacggaaccc
     2761 cgatcctccc gcccatgccg tggcccaaca ccgcgcggct cacgccggac gacatgggct
     2821 cactcatcgc gtacctgaag agcctgccgc cggtcgtgca caaggttccg gccaacctgc
     2881 cgcccaacgg gatggcgcac ggaccggtga tcgagctccc agcgcctggc gcgtgggacg
     2941 cgccgagaac gcagccgcag tgagcccagc cgcgacgacg gcgctccggc cgttccactt
     3001 cgcattccac gtgctcgatc tcgcgtcgac gcgccgcttc tatggcgacg tgctcggctg
     3061 ccgggaaggg cggagcaccg agacgtgggt ggacttcgat ttcttcggca atcagatctc
     3121 ctgccacacg accggccccg tcgcggccac ggcgccaacg gggaaggtcg agaacatcca
     3181 cgtgccgatg cctcacttcg gggccattct cggctgggac gagttccatg ccctcgcgga
     3241 gcggatccgt gcagcgggat tctcgtttgt cgtggagccc cggatccgct atgcggggca
     3301 gcccggcgag caggccacga tgttccttct cgacccaagc ggaaacgcgc tcgagttcaa
     3361 ggcgttccga cacccggagc acgtcttcac gtcatgacgc gcgcgtcggt cgccgtgatc
     3421 ggcgccggcg tggtcggcgc gagcgtggcg taccacctgg cgcgccgcgg atggcgcgag
     3481 gtgctcctcg tggatcgcgc ggaacgtctg ggtctgggga gcacgggcgc ggcgaccggc
     3541 ggctttcgcg cgcagttcgc ctccgacgcg aacgtgcgcc tctcgctcct cgcgcgggag
     3601 cggctctcct cattccaaca ggaaaccggg gtcgatcccg gcctcgtcgc ggccggctat
     3661 ctctggctcg cccggcgcgc ggaggatctc gaggcgctcc ggacggcgct cgccatccag
     3721 agacgaagtg gattcctgga cgcgcgcgag atcggcgcgc gcgaagccgt ggagctgaac
     3781 ccggccatcg atccggatgg agttctcggt ggaacgttcg cggggagcga tggattcctg
     3841 cgccctctcg cgatcctcga aggctacctg cgcgcggcgg aacggctcgg agtcaccgtc
     3901 cgatggaacg ctgaggtcac gggcctgcgg cggggccatg gtggaatcga ggcggtgcgg
     3961 gtcggcgccg attggattct ggtggaagcg gtcgtgaacg cggccggcgc gtgggccgca
     4021 ccgctggccg cgctcgcgga catcaccgtt ccgattgcgc cgctacgacg gcaggccctg
     4081 gcgagcgcgc cattcgcagg tcttcccgag accatgccga tgaccatctg ggtcgacgac
     4141 ggcttccacg cgcgcgtccg cgatggacgt gtgctcgcgc tcatgccgac gcccggcgat
     4201 cccgaggacc cgacgagcac gcgcgtcgat cccgcatggg ttgcgagcgt gactcgcatc
     4261 gcccgggagc gcatccccgt cctccgcgac gctgatttcg aacacggcac ggcgtgggcg
     4321 ggactctacg agatgtcgcc cgacgggcac gcgctgctcg gcgcggcgcc gggatgcgcc
     4381 aacctgttcc tcgcgaacgg ctcctcgggg cacggcgtca tgcacgcgcc ggcgctcggc
     4441 ctcctcgcgg cggagatcct gtcggacggg cgcgcgtcct cgatggacac ggcggcgctc
     4501 gatccgggac gcttcgcggc tgggacggtg gagcggcggg agcttctcta gctcagcgct
     4561 gcaggcaggc gacgtatttc tcgaggaagc gatccatccg ctccatcatg tcgcgccggt
     4621 tcgcctcctg ggcgaatccg tgtccctctc cctcgtagct gaccagctcc accgagccgc
     4681 cgcggcgcct gagcgcgtcc gcgaaatcga ccgactgctg gtaggggacc gccttgtcgg
     4741 ccgtgccgtg aaagaggagc aagggcgctt tcacgcggtc cacgtgggtg atgggggagc
     4801 gctccatcca gacggcaccg gctttgggga gtcgtccgaa gagagactcc tcgtagtgcc
     4861 gctcgaagcg gtgcgcgccg accacggcgt cgtagatgtg cgcgagcggg aagagcgcga
     4921 tgcccgccgt ccagaaatcg ggctcctgcg tgagcgccca gagcgtggcg tacccgcccg
     4981 cgcttccgcc ggtgatcacg acgcggctcc gatcggcgcc gcgcgtcttc agcacgtgct
     5041 ccgcgccgct ccgcgcatcc tcgcgatcca ccacgcccca ggaatcccgg agctggtcct
     5101 ggtacgcgcg tccgtagccc gtgccgcccc ggtggttcac gatgaggtag tgccagcccc
     5161 gcgtggcgta gtactgcgcc tgcgcctgcc acgagaaggg gacttgcgac gtgggaccgc
     5221 cgtgcatcaa tacgatcaaa ggacgcggac cgagcgcggc gtcgggaccc acgggtggat
     5281 agtagatgcc ccaggacacc gccccccccg ccgtcttcca ggagatcact tcgggcttcg
     5341 agaccgactc gggatcgatg aggccgaccg cgctcgaagc gcgcgcggcc tcgtcgccgg
     5401 gccgggcgcc ctgggcgatc cgggtcacga gacgctgggg cgtcgatggg ttcgtcgcgg
     5461 tgtacacgag cgagtgcccg cgaatctgga gtccctggat gtcggtgtac gccgacggga
     5521 tatcctcgac cgcccgcgac gatggatcga tccggagcag cgtgtcccgg ctctgacggc
     5581 tccgcaccgc atagatcgct gtgccgtcgt cgctccaccg atagccggag cgcgccggca
     5641 gccagtccgc gaggccgatc tccccttcgc ccgcgtccac aggcatcgcg ttcgcgccgt
     5701 ccgcgtcgcc gatccatagc gatcgccagc ccgagacgtc gctctggtag gtgaagcgcc
     5761 tcccgtcggg gctccactga ggggcggcga agctcgcgtt ctcctttgcg atggtcgaga
     5821 ggaccgtggc cggagccgcc tcgaagaggg tcgaacacgc ggggatcggc ttcgcgagct
     5881 tcgcgatcac gatccgggac tgatcccacg gcatgtcgag ttgatcccac tcctgccacg
     5941 cgatccgcgc gccatcgggc gaaaacgcgg gattgaacgc gtaccaggga tcgtccgtga
     6001 gctggatcgg gcccgccttg gccttcaggt cggcgagcat cacgttgcag tgcccgtcct
     6061 gttc
//
LOCUS       DASTKC010000029         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_22914, whole genome shotgun sequence.
ACCESSION   DASTKC010000029 DASTKC010000000
VERSION     DASTKC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22914"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VFR25_01030"
     CDS             complement(<1..599)
                     /locus_tag="VFR25_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939662.1"
                     /translation="MTRRTHGVRFRTACAGRRADPLGPAALLRVLFLLFALVLAHPAS
                     AATDASALATALADKHDAGVTRALAPAQACYRDALAQEAAGNTTEALRLLDAASSFDP
                     DFPDAHFTTARILAFRAPDRAVNELSEAFRILARSYPWQRHLLANAATGLVFVWIVGL
                     LLAVVGITLRHLPHLFHVTQEVLARRSTAAVRGAAAAVTL"
     gene            704..2002
                     /gene="asnS"
                     /locus_tag="VFR25_01035"
     CDS             704..2002
                     /gene="asnS"
                     /locus_tag="VFR25_01035"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET9939663.1"
                     /translation="MPSETVRIESLNQHVGQDVTIEGWLYNRRSSGKLEFLQVRDGSG
                     VVQCVASKADLPEDVFARCGTIAQEAAIRVGGSVREDKRAPSGVELTLKDLEAVSGSQ
                     DYPITPKEHGTPFLLDHRHLWIRSQRQHAILRIRHTLIDACRRFLNDEGFINADAPIF
                     TPNACEGTTTLFETQYFDEKAYLTQSGQLYNEATAAAFGKSYCFGPTFRAEKSKTRRH
                     LIEFWMVEPEIAYATLDDVMVFIERFVEAIVARVLAERRPELKILERDVTALERVKAP
                     FPRLSYDEAATRLKDKGLPFEWGGDFGGTDETVLTEGYDRPFFVHRFPAAIKAFYMKR
                     DPSDDRLSLSCDLLAPEGYGEIVGGGQREDDYDTLVSRIQEHQLPMEAFSWYLDLRRY
                     GSVPHSGFGMGIERCLTWICGIEHLREAIPFPRLLNRLRP"
     gene            2049..>2818
                     /locus_tag="VFR25_01040"
     CDS             2049..>2818
                     /locus_tag="VFR25_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9939664.1"
                     /translation="MTSRSALFLLRILVLAVPFLLGAAPSRAAPDRAIYRVSLAGVRD
                     SRALFRLGLDPAGHGPGNTIDFILTPAERDQVRALGYGPVPLATGPSNLAAALSPNFG
                     AYHNLAETMAEMAAYAAAHPSIAVLDTIGFSLEGRPLVAIRISDQAGVNQGEPEALIL
                     GCHHARELMSVELPLYVMRRLLDGYGVDPVLTALVDTRDIWIVPIVNPDGYFYLQSHT
                     SGQSDGWWRKNRRPNGDGTFGVDLNRNYSFRWGWDNIGS"
BASE COUNT          474 a          960 c          947 g          437 t
ORIGIN      
        1 agcgtcacgg ccgcggccgc accccgcacc gcggccgtgg agcgccgcgc gaggacctcc
       61 tgcgtcacgt ggaacaggtg gggcaggtgg cgaagggtga tcccgacgac ggcgagcagg
      121 agtcccacga tccagacgaa gacgaggccg gtcgccgcgt tcgcgaggag atggcgctgc
      181 cacgggtagc tacgcgccag gatccggaac gcctcgctca gctcgttgac cgcgcggtcg
      241 ggggcgcgaa aggccaggat gcgggcggtg gtgaagtggg catcgggaaa atcgggatcg
      301 aagctcgatg cggcgtcgag gagccggagc gcctcggtcg tgttgccggc cgcctcctgc
      361 gcgagcgcgt cccggtagca agcctgcgcc ggagccagcg cgcgcgtgac gccggcgtcg
      421 tgcttgtccg caagggcggt ggcgagcgcg gaggcgtctg tagcggcgga ggcggggtga
      481 gctaggacga gcgcgaagag gaggaaaaga acgcgaagga gcgcggcagg gccgagcgga
      541 tcggctcgac gacccgcgca cgcggtccgg aagcgcactc catgcgttcg gcgcgtcacg
      601 cgcgcacgtt aggcgcgcca ggaaaggagt gtcaaggaaa ttgtccccag agtatccttt
      661 cccgctcatc gtcgacccca acccaagacc ggagagcccg cgcatgccgt ccgaaaccgt
      721 ccgcatcgag tccctgaacc agcatgtcgg ccaggacgtg acgatcgagg ggtggctcta
      781 caaccgccgc tcctccggca agctcgagtt cttacaggta agggacggga gcggggtcgt
      841 tcagtgcgtc gcatccaagg ccgatctgcc cgaagacgtg tttgcgcgct gcggcacgat
      901 cgcgcaggag gcggccatcc gggtgggggg ctccgtgcgg gaagacaagc gcgctccgag
      961 cggggtggag ctgaccttga aagatctcga ggccgtctcg ggcagccagg actaccccat
     1021 cacccccaag gaacacggta ccccgttcct cctcgatcac cgccacctct ggatccggtc
     1081 gcagcgacag cacgcgatct tgcgcatccg ccatacgctc atcgatgcct gccgccggtt
     1141 cctgaacgac gaggggttca tcaacgcgga cgctcccatc ttcacgccga acgcctgcga
     1201 gggaacgacc accctcttcg agactcagta cttcgacgag aaggcgtacc tcacccagag
     1261 cggacagctc tacaacgagg ccacggccgc cgcgttcggc aagtcgtatt gcttcggacc
     1321 cacgttccgc gcggagaaat cgaagacgcg gcgccacctg atcgagttct ggatggtgga
     1381 gcccgagatc gcctacgcga cgctcgacga cgtcatggtc ttcatcgagc gcttcgtcga
     1441 ggccatcgtc gcgcgcgtgc tcgcggagcg ccgccccgag ctcaagatct tggagcgcga
     1501 cgtgacggcg ctcgagcggg tgaaggcgcc cttcccgcgg ctctcgtacg acgaggcggc
     1561 cacgcggctg aaggacaagg gcctcccgtt cgagtgggga ggcgatttcg gcgggaccga
     1621 cgagaccgtg ctgaccgaag ggtacgaccg gcccttcttc gtgcaccgct tcccggctgc
     1681 gatcaaggcg ttctacatga agcgcgatcc gtccgacgac cggctgtcgc tctcctgcga
     1741 tctcctcgct ccggagggat acggagagat cgtggggggt gggcagcgcg aggacgacta
     1801 cgacaccctg gtctcgcgca tccaggagca ccagctcccg atggaggcct tctcctggta
     1861 cctcgatctc aggcgctacg ggtcggtgcc gcacagcgga ttcggcatgg gaatcgagcg
     1921 ttgcctgacc tggatctgcg gtatcgagca cctgagggag gcgatcccgt ttccgcgcct
     1981 cctcaaccgg ctccggccct gacagaagcc ccctcagcgt gatataatga tgggcgccga
     2041 ccgcgcccat gacatctcgt tccgctcttt tccttctccg catcctcgtg ctcgcggtcc
     2101 cgttcctgct cggagccgcg ccgtcccgcg ccgccccaga tcgcgccatc taccgtgtct
     2161 cgctggccgg cgtgagggat tcccgggccc tcttccgcct ggggctcgac cccgcgggtc
     2221 acggtcccgg gaacacgatc gacttcattc tcacgcctgc cgagcgcgac caggtgcgcg
     2281 cgcttggata cggtcccgtt ccactcgcga cggggccctc caacctcgcg gccgcgctca
     2341 gtcccaactt cggcgcgtat cacaatctcg ccgagaccat ggcggagatg gcggcgtacg
     2401 ccgcagccca cccctcgatc gcggtcctcg acacgatcgg gttctcgctg gaggggcgcc
     2461 cgctcgtcgc catccggatc tccgaccagg cgggagtgaa ccagggcgag ccggaggctc
     2521 tgatccttgg ctgtcaccac gcccgggagc tcatgagcgt cgagctcccg ctctacgtca
     2581 tgcgccggct cctcgacggc tacggcgtgg atcccgtgct gacggcgctc gtggacacgc
     2641 gcgacatctg gatcgttccc atcgtgaatc cggacggata cttctacctc cagagccaca
     2701 cgagcggcca gtcggacggc tggtggcgca agaaccgccg gcccaacggc gacggaacgt
     2761 tcggcgtcga cctgaaccgg aactactcct tccggtgggg atgggacaac atcgggtc
//
LOCUS       DASTKC010000030        19161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1678464, whole genome shotgun sequence.
ACCESSION   DASTKC010000030 DASTKC010000000
VERSION     DASTKC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678464"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1117)
                     /gene="recF"
                     /locus_tag="VFR25_01045"
     CDS             complement(14..1117)
                     /gene="recF"
                     /locus_tag="VFR25_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET9939665.1"
                     /translation="MRLLSLELRDFRNHHAAWAPIDGASTLLLGENGSGKTNWIEAAV
                     LLSIGRSFRGARDRDIARRGASRFEVRGTVANRAGVRCDIAARSMGAGPREVRVDGQP
                     LPRLGELLGRFPTVHFSVEDVGAWNGAPAGRRRFLDVALCQLDPAYVAHLREYTGALR
                     QRNRLLAERGPEDPDQLGAWEEILARSGAELDRRRGAVVAELNGTLRGLAGQVDPGLE
                     PSIEYPAEAPGDGDAVAERARKLAAHRSRDRRLGWTADGPHRAPVSCRLAGRELVEGA
                     SRGMTRLYSILLRLALARVIEARLEEPPVVFLDDPESELDERWIGPVLELVPERSQVI
                     VTACRPLTAYPERFREMTIASLVRETAVEEVGS"
     gene            complement(1128..2264)
                     /gene="dnaN"
                     /locus_tag="VFR25_01050"
     CDS             complement(1128..2264)
                     /gene="dnaN"
                     /locus_tag="VFR25_01050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET9939666.1"
                     /translation="MKFTVAQSEIQSALQLVSSAVPSRTTLPVLSNILIEALDGSIQM
                     TATDLDLAIATKATADVKGEGSLTVPAKRLFELVRKLPREELKFEAKDLTLNLGSKTG
                     RFKFNCIRPEEFPARITVNPELTFRIEPKLLEHMVRRSIYAVSTDETRPALNGALLQI
                     VEGEVRLVATDGHRLARATVKQAGIGKSPLKGDVIVPLKALNHLLRLLTDAPDTVTVE
                     LSKNHARFAVGPTTLTTKLIEGPFPNYEQVLPKQNTKILRAGREELTQALDRVSLFSD
                     NLTRQVKFSITNNKLRLVVQTPDQGEANEEIDVRYTADDLDIGYNASYILDILRTIDS
                     EEIVMQLNTPVTAGLVMPAAKDDAKGAPSQEDLLCLVMPLRLAG"
     gene            complement(2664..3197)
                     /locus_tag="VFR25_01055"
     CDS             complement(2664..3197)
                     /locus_tag="VFR25_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939667.1"
                     /translation="MVAMDGAHAEELLADLDLAVAELERAADRGWDRGLPGKWTAGEH
                     VAHIAATMAETADSFEKGAALLRAGFLPQHPRRGPMQWLWVHLVIAPGRLPRGGKTAA
                     RFVPANQPNRSTTLRELQQAAARHRTLTQSLSTRERDRLWIANPFLPRWHYTLPEILR
                     AHAVHARHHARLIAEIP"
     gene            complement(3272..4600)
                     /gene="dnaA"
                     /locus_tag="VFR25_01060"
     CDS             complement(3272..4600)
                     /gene="dnaA"
                     /locus_tag="VFR25_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9939668.1"
                     /translation="MMETMVAGDLWEQALQRIQSNLPSQTYETWFRSLGPLEFDGSTL
                     VLEAPSQFYVDWLDQHYRPLIESSVAAAADRSVGISFHVRPGRPVVEEERPRGPIPTR
                     DESNLSPNNTFETFIVGSGNQFAHAVCQAVAQSPGERYNPLFIYGGVGLGKTHLMHAI
                     GHYVREQRRGARVFFVTAEKFMNEMIYSIQHATTLEFKSRYRTADVLLIDDIQFLAGK
                     ESTQEEFFHTFNTLHGAHKQIVLTSDGPPNSITALEERLISRFTWGVIADLQPPDLET
                     RVAIVKKKAELQRRSLPNEIALLLASNIKTNIRDLEGSLARLLAFAELTGQPLTVEFA
                     QEVLRDQIKPDLARIDALDIQRAVARHFGVSEESLRGKRRTDTIAFPRQIGMYVTRSI
                     TDMSLAEIGGKFGGRDHTTVLHACQKIEKLMQTDRDLRTVVEDLMASLSR"
     gene            5045..5179
                     /gene="rpmH"
                     /locus_tag="VFR25_01065"
     CDS             5045..5179
                     /gene="rpmH"
                     /locus_tag="VFR25_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9939669.1"
                     /translation="MKRTFQPHNRRRKKVHGFRERMSTRGGRAVLKRRRDRGRKRLTV
                     "
     gene            5205..5552
                     /gene="rnpA"
                     /locus_tag="VFR25_01070"
     CDS             5205..5552
                     /gene="rnpA"
                     /locus_tag="VFR25_01070"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9939670.1"
                     /translation="MAGAVPPRERRLQAGWRFREVYRSGKPFHGSLMTLVSLERQDDH
                     GRVAFVASRKVGSAVKRNRAKRLLREAFRATPDVQREALRWRVWIARATCAESVLAQV
                     SDEMRRLLLREAK"
     gene            5549..5761
                     /gene="yidD"
                     /locus_tag="VFR25_01075"
     CDS             5549..5761
                     /gene="yidD"
                     /locus_tag="VFR25_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009786895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9939671.1"
                     /translation="MKQLFLALIRGYRLLLSPLLPRACRFHPSCSEYAEEAFRTLPLH
                     QALYRSARRVLKCHPFNPGGYDPVQH"
     gene            5777..7444
                     /gene="yidC"
                     /locus_tag="VFR25_01080"
     CDS             5777..7444
                     /gene="yidC"
                     /locus_tag="VFR25_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9939672.1"
                     /translation="MERRTLLAIFLIMAVLIGDQIWLSQSAKKRKAPAGVDTTQVATR
                     ADTTRPGLIAPPPTPATGGGSTAAGGSAPAPGGALASHPRTVPAPETTHVIRTPYYVA
                     TFSSKGGVIAHWMVPGYKDLSAPQKDVDLAEPSRAMRLVVKTPYFDYDFTDAPFRVES
                     ASDTAVTFVADDSSGVRVTKSYRVASDPRAMDIEVRAAVPAPYGPIQMKWGWGESLPH
                     TEHMMTPRTVAAVALIGDKMERMDAAHIQKDGNKALQGNVRWAAVRSKYFVAALIPDS
                     ATVGAVEFTQSEQKEATVWLVDAAPPGTDLRRHMRLYAGPIHYDTLVAQGSELDRLAN
                     LGWRWITGVSALLLWCLNALYKLIPNYGIAIILLSAATKLVFYPLTQASLRSMKVMHH
                     LQPEMKALQERYQSDPARMNKEVMDLYKRYKVNPLSGCLPMIVQIPVFFALYNVLLNS
                     IELRGAGFLGYIADLSVPDVLMRVAGFPIHLFPILMTGSSYYMQSQTPVSPQQKPTMM
                     LMPVMMLVFMYNFPSGVVLYWTVTNVLSGVQQYLVNLAEDRKMAASA"
     gene            7447..8289
                     /locus_tag="VFR25_01085"
     CDS             7447..8289
                     /locus_tag="VFR25_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET9939673.1"
                     /translation="MRSHGHDQPIETAVEAEAPTFGEAVNDAAVRLGVSTADLAIEIL
                     DPGAGEGSSTGFRPVKIRARRRAAHSAPDGARPKPPGERRPFERGGYDRGGRGPREAT
                     YRRPEPVNYGPPPVPMDPSKITPEILEMVRGLADGMRDSMEFEGTVTAEKTSHGIRIG
                     IDAGENDQFLIGKDGDTLSAFQHLLGRMVRAKMPDEAPPRIEVDVAGYRDRQIEKLRE
                     LARELMQEAKDTGAEVTTEPLPASERRIVHLEVAEDKEMETVTIGDGYYKRVVVRRAT
                     GEPK"
     gene            8305..9684
                     /gene="mnmE"
                     /locus_tag="VFR25_01090"
     CDS             8305..9684
                     /gene="mnmE"
                     /locus_tag="VFR25_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET9939674.1"
                     /translation="MANDTIVAGITAPGDSAVAVTRVSGPRALELVAARFRGRRSPLS
                     SPSHRILFGSFLGDDGAPVDTVLLSVFRAPHTYTGEDVAEISSHGGAAIPRAIFDALV
                     ADGARPARPGEFTERAYRNGKLDLAQAESVAALVRARSERSARAASLALGGALSRRVE
                     AWDGALIAVLAEVVSRIDFPAEVFEDVDGEALAESCETTIREIDELLARLPGSRRVQE
                     GIRVSMVGRPNVGKSSLLNSLLGYDRAIVTEHPGTTRDTLEDSIWLDGVELRLTDTAG
                     VRAVDDPVERLGVERAEGAVRACDLAVVVLDRSLPETESASHASELMTLASGRPVIVA
                     WNKADLKRGSEARGAVLDAMAEVETVAIHAGGAEPLRDALRSAIPFMLGAHAGEEIAT
                     TSARQEGLLAEARGALERASSGLREERSYDLVAVDLEDARRALAEVRGRGVDDQVVAA
                     IFSRFCIGK"
     gene            9705..11645
                     /gene="mnmG"
                     /locus_tag="VFR25_01095"
     CDS             9705..11645
                     /gene="mnmG"
                     /locus_tag="VFR25_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET9939675.1"
                     /translation="MAKSFPLLVIGAGHAGCEAASIAARLGIRVGLVTMSLDAIAHMP
                     CNPAIGGLAKGHLVREIDALGGLMGRMADQAGIQFKLLNRGRGPAVWSPRAQEDKARY
                     RALVRAHLESTPEIEVVQEQVVALEVAGGRVRGVRLANGAVIGAERVIVTPGTFLNGL
                     MHVGERTIAGGRVGERAARGISDCLTDLGFRLVRLKTGTPPRIHRDSIDFDRMLPQRG
                     DDPPRPFSHFTDELVVDQIECHLTGTTDATHRAVRENLQHSPLYSGRIRGIGPRYCPS
                     IEDKVVRFADKPTHQIFIEPEGRDVDDFYINGLSTSLPEEIQLQILATIPGLEHARMI
                     RPGYAVEYDFIPPTQLHPTLETRLVRGLYLAGQINGTSGYEEAAAQGLMAGINASLSL
                     MEREPMVLRRDEAYIGVLLDDLVTKGTEEPYRMFTSSAEFRLLLRQDNASERLLGRAR
                     ELGALRDEEVEQLEDSVRRRVAAAERMRTMKVACVAHAATAAAVASVGAAASLAAAAS
                     AAPTASARAVSPEELVSIATALRDGRMSVEESCARPELADLGTEAVESAAIEIRYEGY
                     IARQKREVERARRGEQLALPDCVFDEPLNQMSREGREKLRRVRPSTLAQASRIPGMSP
                     ADMSVLLIYAERERRRAGLRPA"
     gene            11602..12294
                     /gene="rsmG"
                     /locus_tag="VFR25_01100"
     CDS             11602..12294
                     /gene="rsmG"
                     /locus_tag="VFR25_01100"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET9939676.1"
                     /translation="MPSASGGARGSVPPDLESDFAAFRRAVQPWLTSVRGASADGELV
                     ESLKKFGLLVHERAASLSLIAQGDRPVLFTRHILDSLNLATITEAPPASLLDVGSGAG
                     FPGIPLAILWPETRVTLLESRDRKAGFLELAVRHLGLRHVRVVCDRLERVSQDRSIDP
                     VGAVTVRAVGDLPRLLTLAAPLATADAWWAYFVGGGLAEDDARTLAPEARVETGAFGG
                     RILRGSFRRSDS"
     gene            12728..13489
                     /locus_tag="VFR25_01105"
     CDS             12728..13489
                     /locus_tag="VFR25_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9939677.1"
                     /translation="MTRTVAIANQKGGVGKTTTAVNLAAALALAQKKVLLVDLDPQGN
                     AGSGLGHPAVKVGRGIYEVLTTDLPIQDVLLETEVPGLSLVPSGQRLAAAEVELVDVE
                     RREFRLKDSLSKIEDRFDFIIIDAPPSLGFLTVNALTGAKSVLVPVQCEYYALEGLTH
                     LLGAIRLVQQSLNPTLQIEGVLLTMYDNRLNLSQQVLEETRRFFAERVYRTVIPRNVR
                     LSEAPSFGKPVVIYDPGCVGSTSYINLAREVVERG"
     gene            13617..14405
                     /locus_tag="VFR25_01110"
     CDS             13617..14405
                     /locus_tag="VFR25_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9939678.1"
                     /translation="MSLTKIVRNPVQPRTQFDPATIKELADSIRERGVLQPVLLRPAG
                     EGYQIVAGERRFLAAREAGFTTIPSIVRPLTDRESLLIALIENIQRENLNPIDEARAY
                     YRLATEYGLQHDEIGARVGKDRSTVSNLIRFNNLPDTVQSLLESGSISGGHARALLGL
                     SDAKSQVDLANSAASHGWSVRELEDRVKRSGPARAPRPRRAPKRAFSGELMAVEDELM
                     RVMGAKVRITRRRGGMGSILIDYHSEEELERLIGLFRDLAATPR"
     gene            14577..15593
                     /locus_tag="VFR25_01115"
     CDS             14577..15593
                     /locus_tag="VFR25_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9939679.1"
                     /translation="MAQPVATSDVQLLEELKNARGEILGELRKVIVGQDAVVEQLLTV
                     LFANGHCLLVGVPGLAKTLLVSTLATILDLKFSRIQFTPDLMPSDITGTEILEDDPVS
                     GKRTFKFIRGPIFANMVLADEINRTPPKTQAALLQSMQEYRVTAGGTTYPLDLPFFVL
                     ATQNPIEFEGTYPLPEAQLDRFMYHISVGYPTDAEEEKIVATTTSAYEASPKKVMGVQ
                     RILDLQHLVRRIPVSEHVVRYAVRLARASRPGPESASFVRDWVSWGAGPRAAQYLVLG
                     AKARAAIQGRPTPGAEDVRSVAPAVLRHRIIPNFNAEADGISSLDIIAKLLETTPSVD
                     GKHA"
     gene            15606..16502
                     /locus_tag="VFR25_01120"
     CDS             15606..16502
                     /locus_tag="VFR25_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9939680.1"
                     /translation="MSERRPRLLDPHFVSKLTRLDLTARLVVEGFLTGLHRSPYHGFS
                     VEFAEHRQYMPGDPLRHLDWRVLAKSDRKFIKQYEEETNLRAMLLVDTSASMGYGSHG
                     VTKLDYARQLAAALAYLMLRQNDAVGMFAFATGRAELIPPRSTLGHARPLLLLLERLT
                     PGGATDFASSLHSLAERMNRRGLVVLISDLLDDPERIAQAIHHFRHKKHEVLVFHVLD
                     PQEVAFDFEREAVYVDLETGERVTTRPQELRPDYRARVSAWRDQIRQFCIEKRAEYVP
                     LTTDQPFDRALLEYLSKRARLL"
     gene            16515..18620
                     /locus_tag="VFR25_01125"
     CDS             16515..18620
                     /locus_tag="VFR25_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HET9939681.1"
                     /translation="MPVFSFLNSGFLAALPLASIPVIIHLLSRRRLPEVPFPTTQFLR
                     QLEPREIRRLRLREILLLILRTLAIILLVLAFARPSITPRNAVTTAAAAMAVIIDDSE
                     SMAGLDEQAKPRMDGAIERARALVLAARPGDEMTLISATRPIGAATEHTSDRVRLGRS
                     LANTQVTMLPADMAGALLMARRTLERSRLRDRELYIISDLQRSNFDAKARAELGPIAA
                     AKIRVYLVPVVQSRVPNHALVDLDPVLRPGPMGRGLELRSRIANYADAPSDRIAIRVR
                     HGDALVGGGDVTLKADEIRWAAMPLDWRGAGFDSSQSSGAVLIEADQDALAPDDRWFA
                     VLGAPGRLRVLRVTEPREGAAPPRFASLALDPGKDGRSGFVVDEVSPAGLLGLTKSRY
                     DVVLIEDVASLSTEGEARVRAFLRDGGGLVVALGPHSDPGYYGSRLFPGVIDLGLDGP
                     EQAAAGGSYELRARLPGHDVLNGLNVGVGAPLTESRLTGLMKARTLTTRPEVVVQTTG
                     GLPLVATSPGVAVFLSSLSDDWGDLPYSGAFVPLVRGLVSYAAQADAQGPGSTRVGEA
                     PAARLTSPPGAAVIVRGPESYSSQASVRAEGTAYRAEADAPAVKPGFYDFEAGGRSIA
                     TVAVNVSPVESDLAVIDADSLRAASGSGSSTFRSANALATHLRDTRRGKELWMTFLLL
                     AAAALAGELLLGSARALRA"
     gene            18617..>19161
                     /locus_tag="VFR25_01130"
     CDS             18617..>19161
                     /locus_tag="VFR25_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939682.1"
                     /translation="MSAPGPLPRRLVEVAARHPALAALASRVGAPSGRPAHVRIRGAS
                     GSSGALTLAALLEGSTRPMAVIAPDIDRAEAWRDDLEFLLGEERVVYLPPLDVVAWSH
                     QLATAPVRDDRLQAIVRLGDPDPPVAVLPAAALHRLVPKPDRVRARSITLTSGYEADP
                     EGLVRRLVVGGYRAVAEIGESG"
BASE COUNT         3022 a         6603 c         6407 g         3129 t
ORIGIN      
        1 tcccgcggcc gggtcacgag cccacctctt ccaccgccgt ctctcggacg agactggcga
       61 tcgtcatttc ccggaatcgc tccggatacg ccgtgagcgg ccggcaggcc gtgacgatga
      121 cctggctccg ctccggcacc agctcgagca ccgggccgat ccatcgttca tccaactccg
      181 actcgggatc gtcgaggaag acgaccggcg gctcctcgag cctcgcctcg atcacccgcg
      241 cgagcgcgag ccgaaggagg atcgagtaga gccgcgtcat cccgcgcgac gctccttcga
      301 ccagctcgcg acccgcgagc cggcagctca ccggcgcgcg gtgcggtccg tcggccgtcc
      361 agccgagtcg acggtcgcgc gagcgatgcg ccgcgagctt ccgcgcgcgc tccgccacgg
      421 cgtctccgtc ccccggcgct tcggccgggt actcgatcga cggctcgagc cccggatcca
      481 cctgcccggc cagcccgcgc agcgtcccgt tcagctccgc gaccaccgcg ccgcgcctgc
      541 gatccagctc cgcgccggag cgggcgagga tctcctccca cgcgccgagc tgatcgggat
      601 cctcgggacc ccgctcggcg agcaggcggt tccgctggcg gagcgcgccc gtgtactccc
      661 ttagatgcgc cacgtaggcc gggtcgagct ggcagagcgc cacgtccagg aaccgccgcc
      721 gtcccgccgg cgcgccgttc caggccccga cgtcctccac ggagaagtgc accgtgggaa
      781 agcgtcccag gagctcgccc agcctcggca ggggctggcc gtccacgcgg acctcgcgcg
      841 gcccggcgcc catggatcgc gccgcgatgt cgcaccggac tcccgcccgg ttcgccaccg
      901 tgccacgcac ctcgaaccgc gaggcgcccc gccgcgcgat gtcgcgatcc cgcgcaccac
      961 ggaacgagcg cccgatcgag agcaggaccg cggcttcgat ccagttggtc tttcccgagc
     1021 cgttctcgcc gaggagaagg gtcgaggcgc cgtcgatcgg cgcccaggcc gcgtgatgat
     1081 tcctgaagtc acgaagctcg agtgacagga gcctcacgcg gtgggaatca cccggccagt
     1141 cggagcggca tgacgaggca gagcaggtcc tcttgcgagg gcgcaccctt ggcgtcgtcc
     1201 ttggccgcgg gcatcacgag acccgcggtg accggcgtgt tcagctgcat cacgatctcc
     1261 tccgagtcga tcgtgcgcag gatgtcgagg atgtagctcg cgttgtatcc gatgtcgaga
     1321 tcgtcggccg tgtagcggac gtcgatctcc tcgttggctt ctccctgatc cggcgtctgg
     1381 accacgaggc ggagcttgtt gttggtgatc gagaacttga cctgtcgcgt caggttgtcc
     1441 gagaagaggg acacgcgatc cagcgcttgg gtcagctcct cgcgccccgc gcgcaggatc
     1501 ttggtgttct gcttcggaag cacctgctcg tagttgggga acgggccctc gatcaatttc
     1561 gtcgtgagcg tcgtcggccc caccgcgaac cgggcatggt tcttcgagag ctccacggtc
     1621 accgtatccg gcgcatcggt gaggagccgg agcagatgat tgagcgcctt cagcggcacg
     1681 atcacatcgc ccttgagcgg gctcttgccg atccctgcct gcttcacggt cgcgcgggcg
     1741 agccgatgtc cgtcggtcgc cacgagtcga acctcgcctt cgacaatctg taagagtgct
     1801 ccgttcagcg ccggccgggt ctcgtcggtg gagaccgcgt agatcgaccg ccgaaccatg
     1861 tgctcgagga gcttgggctc gatccggaac gtgagctccg gattcaccgt gatccgcgcc
     1921 ggaaattctt ccgggcggat gcagttgaac ttgaagcgtc cggtcttgct cccgagattc
     1981 agcgtcaggt ccttggcttc gaacttgagc tcttctcgtg gaagctttcg aacgagctcg
     2041 aagagacgct tcgccggaac ggtgagcgag ccttcgccct tcacgtcggc cgtcgctttc
     2101 gtcgcgatcg cgaggtcgag atcggtggcc gtcatctgga tcgatccgtc cagagcttcg
     2161 atcaagatgt tcgacagaac gggaagggtc gtccgacttg gaacggcgct cgaaacgagc
     2221 tgcaacgcgg actggatttc ggactgcgcg acggtgaatt tcatagccac atcctgggca
     2281 gtgatcatgt cgcttaatcc tttggacggt ttagggttgc ggtcatgtcg aggatggggt
     2341 cggtcgctgg atttcagtat aggttttggg gggtcgaatc acaacaggga aagccttcat
     2401 ttctccacaa tatggggcct gcctacagaa cattagattc cttgaatggt ggtactagaa
     2461 gtaggggctg ttgagcgtgg ggatgggtcg cgggagggtg agagtaagtt gttgtagggg
     2521 tggtaaatgc aggtccttgg tccgcgcgcc gatcgcttgc gttcagtggg gacaaggtga
     2581 tggttatccc cgcgttccgc gcgtgggagc gaatgcgcgg cgtggtccac gcactatcca
     2641 caccgctccg tgacggacga cgctcaagga atctctgcga tgagcctcgc gtgatggcgc
     2701 gcgtggacgg cgtgagctcg gaggatctcc ggcagcgtgt agtgccagcg cggaaggaag
     2761 ggattcgcga tccagaggcg gtcccgctca cgggtggaga ggctttgcgt gagggtccgg
     2821 tgccgcgccg cggcctgttg gagctcgcgg agggtggtcg agcgattggg ctgattggct
     2881 ggaacgaatc gcgccgcggt ctttccgccg cgcgggagcc ggccaggcgc gatcacgagg
     2941 tgcacccaga gccactgcat gggcccgcgc cgggggtgtt ggggcaagaa tcccgcccgg
     3001 agcagcgccg ctcccttttc gaagctgtcc gccgtctccg ccatggtcgc cgcgatgtgt
     3061 gcgacgtgct cgcccgccgt ccacttcccc ggaaggcccc gatcccagcc tcgatccgcc
     3121 gcccgctcga gctccgccac cgccaagtcg agatcggcga gaagctcctc cgcgtgcgcg
     3181 ccgtccatcg ccaccatgcg ccctccagaa acgcgaaacg ggccgcccca tcaggagcgg
     3241 cccgtcccag gatagcagtc tcgaggggcg tctagcggct caggctcgcc atcagatcct
     3301 cgaccacagt tctcaagtcg cggtcggtct gcatgagctt ctcgatcttc tggcaggcgt
     3361 ggagcacggt cgtgtgatcc cgcccgccga acttgccgcc gatctcggcc agggacatgt
     3421 ccgtgatcga ccgggtgacg tacatcccga tctgccgtgg gaaggcgatc gtgtcggtcc
     3481 ggcgcttgcc gcgcagggat tcctcgctca cgccgaagtg ccgcgccacc gcccgctgga
     3541 tgtcgagggc gtcgatccgg gccaggtcgg gcttgatctg gtcccgcagc acctcctgcg
     3601 caaactcgac cgtcaagggc tgcccggtca gctccgcaaa ggcgaggagc cgcgcgaggg
     3661 atccctcgag gtcccggatg ttcgtcttga tattgctcgc gagaaggagc gcgatctcgt
     3721 tcgggaggct ccggcgctgg agctcggcct tcttcttcac gatggcgacc cgggtctcga
     3781 ggtctggcgg ttggaggtcc gcgatcaccc cccaggtgaa ccgggagatc agccgctcct
     3841 cgagcgcggt gatcgagttg ggcgggccgt cgctcgtgag gacgatctgc ttgtgggcgc
     3901 cgtggagcgt gttgaaggtg tggaagaact cttcctgcgt gctctccttg ccggcgagga
     3961 actggatgtc gtcgatcagg agcacgtcgg cggtccggta gcggctcttg aactcgagcg
     4021 tggtggcgtg ctggatcgag tagatcatct cgttcatgaa tttctcggcc gtcacgaaaa
     4081 agacccgcgc cccgcgccgc tgctcccgga cgtagtgccc gatcgcgtgc atcaggtgcg
     4141 tctttccgag ccccacgccc ccgtagatga agagcgggtt gtaccgctcg cccgggctct
     4201 gggccacagc ctggcagacc gcgtgggcga actggttgcc gctccccacg atgaaggttt
     4261 cgaacgtgtt gttcgggctg aggttgctct catcgcgcgt cggaatcggc ccgcgggggc
     4321 gctcctcctc cacgaccgga cggccaggcc gcacgtggaa cgagatcccc accgagcggt
     4381 ccgccgcggc cgccaccgag gattcgatca gcggccggta gtgctgatcg agccagtcca
     4441 cgtagaactg gctaggggcc tccaggacga gggtcgaccc gtcgaactca agcgggccta
     4501 gtgatcggaa ccaggtttcg tacgtttggc ttgggagatt ggattggatc cgttgaagcg
     4561 cttgttccca cagatcgccg gctaccatcg tttccatcat cggggcatcc ccatcgacac
     4621 acaccctctc cacgcatgat ccacatagtt atccccaaag aaacaagggc gcaacggatt
     4681 gcgtgtcgac ccgttgcgcc caaaggcata cgaatgtggc gttgtgttct tcaccgcatg
     4741 ttggacccgg atgaaaggcc gcgcgcgcag cgcgcgtgag ggagaagctc ggacctggtt
     4801 gcgggcccag ggggcgctca ccacccccac ggcccacgac cggccgggaa tttacgtccg
     4861 cggttacaag gtcgcaacga aaaaatcaga gacacgcgac acttcgattg ccagcgagcc
     4921 tgaaacacgg tccgaggcgg gcttcgatgg gcccctcgct ccccgttgac cgggcttggc
     4981 gcatcgggta gactcccacg ctcgatatcg aacaagccgt ttcccaagaa cgaggccatt
     5041 cacagtgaag cgcacgtttc agccgcacaa ccgccgccgg aagaaggtcc acggattccg
     5101 cgagcgtatg agcacccgcg gaggccgcgc cgtattgaag cgccgccggg accgcggccg
     5161 caagcgtctc acggtctgat ccgatccctc cggacgtatc gtcgatggcc ggcgcggttc
     5221 caccgcgcga gcgtcgcctt caggcggggt ggcggttccg cgaggtgtat cgctcgggga
     5281 agccgttcca cggctcgctg atgaccctcg tcagtctgga gcgtcaggac gaccacggcc
     5341 gcgtcgcgtt cgtcgcgagc cggaaggtgg gatccgccgt gaagcgaaat cgcgccaagc
     5401 gtcttctccg ggaggcgttc cgcgccacgc ccgacgtcca gcgggaagcc ctgcgctggc
     5461 gggtctggat cgcgcgcgcc acgtgcgcgg agtccgtgct cgcccaggta tcggacgaga
     5521 tgcggcggct cctgctccgg gaagccaagt gaagcaactc ttcctcgccc tgatccgcgg
     5581 ctacaggctc ctcctctcgc cgctcttgcc gcgggcctgc cgcttccatc cgagctgctc
     5641 cgagtacgcc gaggaggcgt tccggacgct tccgctccat caggcgctct accggagcgc
     5701 tcgccgcgtc ctcaagtgcc atccgttcaa ccccggagga tacgacccgg tccagcacta
     5761 ggccggcacc gactccatgg aacggagaac tctgctcgcg atcttcctga tcatggccgt
     5821 cctgatcggg gaccagatct ggctgtcgca atcggcgaag aagcggaagg cgcccgccgg
     5881 cgtggacacg acgcaagtcg cgacgcgcgc ggacacgacg cgcccgggcc tcatcgcgcc
     5941 tcctccgaca cccgcgacgg gtggcggctc gaccgcggcc ggcggaagcg cgcccgcgcc
     6001 gggcggcgct ctcgcctcgc acccgcgcac cgtgcccgcg cccgagacca cccacgtcat
     6061 ccggaccccc tattacgtcg cgaccttctc gtcgaagggg ggcgtgatcg cgcactggat
     6121 ggtcccgggt tacaaggacc tctccgcgcc gcagaaggac gtcgacctcg ccgagccgtc
     6181 ccgcgcgatg cggctggtcg tgaagacgcc gtacttcgac tacgacttca ccgacgcccc
     6241 gttccgcgtc gagtccgcct ccgacaccgc ggtcacgttc gtcgcggacg actcgtcggg
     6301 cgtgcgcgtg accaagagct atcgcgtcgc gagcgatccc cgcgcgatgg acatcgaagt
     6361 ccgcgcggcc gtgcccgctc cctatgggcc gatccagatg aagtggggct ggggcgaatc
     6421 cctgccgcac acggagcaca tgatgacgcc gcgcaccgtc gcggcggtcg ccctgatcgg
     6481 cgacaagatg gagcgcatgg atgccgccca catccagaag gacgggaaca aggcgctcca
     6541 gggcaacgtc cggtgggcgg cggtgcggag caagtacttc gtcgccgcgc tcattcccga
     6601 ctccgccacc gtcggcgccg tcgagttcac acagagcgag cagaaagaag ccaccgtgtg
     6661 gctcgtggac gcggctccgc ccggcaccga tctccgccgc cacatgcggc tctacgcggg
     6721 acccatccac tacgacaccc tcgtggccca gggctccgag ctggaccggc tcgcgaacct
     6781 cgggtggcgc tggatcacgg gcgtgagcgc gctccttctc tggtgcctga acgcgctcta
     6841 caagctgatt ccgaactatg ggatcgcgat cattctcctc tcggcggcga cgaagctcgt
     6901 cttctacccg ctcacgcagg cctcccttcg ctcgatgaag gtgatgcacc acctgcagcc
     6961 cgagatgaag gcgctccagg agcgctacca gagcgatccc gcgcggatga acaaggaagt
     7021 gatggacctc tacaagcggt acaaggtcaa tccgctgagc ggctgccttc cgatgatcgt
     7081 ccagatcccc gttttcttcg cgctctacaa cgtgctcctg aactcgatcg agctccgcgg
     7141 cgccgggttc ctgggctaca tcgcggacct ctcggtgccg gacgtgttga tgcgggtggc
     7201 cgggttcccc atccacctct tcccgatcct catgacgggg agcagctact acatgcagtc
     7261 ccagaccccg gtctcacccc agcagaagcc gaccatgatg ctcatgccgg tcatgatgct
     7321 ggtcttcatg tataatttcc catccggcgt ggtcctgtac tggaccgtga ccaacgttct
     7381 ctccggagtt cagcaatacc tggtgaacct ggcggaagac cgaaaaatgg ccgcgagcgc
     7441 gtagcgatga gatcccacgg acacgatcag cccatcgaga ccgcggtcga ggcggaagcc
     7501 ccgaccttcg gcgaagccgt gaacgacgcc gccgttcgcc tcggcgtcag cacagccgac
     7561 ctggcgatcg agatcctcga tccaggggcc ggcgaagggt cgagcaccgg cttccggccg
     7621 gtgaagatcc gcgcccgccg gcgcgccgcg cactcggcgc cggacggcgc gcgccccaag
     7681 cctcccggcg agcgccgccc cttcgagcgc gggggctacg atcgaggggg acgcggcccg
     7741 cgcgaggcga cgtatcgacg tcccgagccg gtcaattacg gccctccgcc cgtgccgatg
     7801 gatccgtcga agatcacgcc cgaaatattg gagatggtcc ggggactcgc cgacgggatg
     7861 cgcgacagca tggagttcga aggtacggtc accgcggaga agacctcgca cggcatccgc
     7921 atcggcatcg acgccggcga gaacgatcaa ttcctcatcg gcaaggatgg cgacacgctc
     7981 tccgcgttcc agcacctcct cggccgcatg gtccgggcca agatgccgga cgaggcgccg
     8041 cccaggatcg aggtggacgt cgccggatat cgcgaccgcc agatcgagaa gctgcgcgag
     8101 ctcgcgcgcg agctgatgca ggaagcgaag gacacggggg ccgaggtcac caccgagccg
     8161 ctgcccgcgt cggagcggcg catcgtccat ctcgaggtgg ccgaggacaa ggagatggag
     8221 accgtcacca tcggcgacgg ctactacaag cgcgtcgtgg tgcggcgcgc gaccggcgaa
     8281 cccaagtgaa cgcgcggggc gcaggtggca aacgatacga tcgtagcggg gatcacggcc
     8341 cccggagatt cggccgtggc ggtcacgcgc gtcagcggac cgcgtgctct cgagctggtc
     8401 gccgcgaggt tccgcggacg ccgctcgccg ctttctagcc cctcccaccg cattctcttc
     8461 ggatccttcc tcggggacga cggcgccccc gtcgataccg tcctgctctc cgtgttccgc
     8521 gccccacaca cttacaccgg cgaggacgtg gcggagatct cgtctcatgg cggcgcagcg
     8581 attccgcgtg ccatattcga cgccctggtt gcggacggtg cgcgtccggc gcggccgggc
     8641 gagttcacgg agcgcgccta ccggaatggg aagctcgact tagcacaggc ggaatcggtc
     8701 gcggccctcg tccgtgcgcg ctcggagcgc tccgctcgcg cggcgtcgct cgctctgggc
     8761 ggcgcactgt cgcgcagggt ggaagcgtgg gatggggcgc tcatcgccgt gctggccgaa
     8821 gtggtgtcgc ggatcgattt ccccgcggaa gttttcgagg acgtcgatgg tgaggctctg
     8881 gccgagtcgt gcgagacgac gatccgcgag atcgacgagc tcctggcgcg cctccccggc
     8941 tcgcgccgag tgcaggaggg gatccgagtc tccatggtcg gccgccccaa cgtcggaaag
     9001 tcgagcctcc tgaactcgct tctcggatac gaccgcgcga tcgtgaccga gcatcccgga
     9061 acgacccgcg acacgctcga agactcgatc tggctcgacg gcgtcgagct tcgattgacc
     9121 gacacggccg gcgtgcgcgc ggtggacgat ccggtcgagc ggctcggcgt cgagcgcgcg
     9181 gaaggcgcgg tgcgcgcgtg cgatctcgcg gtggtcgtcc tcgaccgctc gcttcccgag
     9241 acggagagcg cgtcgcacgc atcggagctg atgaccctcg caagcggacg gccggtgatc
     9301 gtcgcgtgga acaaggccga tctgaagcgc gggtcggagg cgcggggtgc ggtgctcgat
     9361 gcgatggccg aagtcgagac cgtagcgatc cacgcgggcg gcgcggagcc gcttcgcgat
     9421 gcgcttcgct ccgccattcc attcatgctc ggcgcgcacg cgggcgagga gatcgcgacg
     9481 acgagcgcgc gccaggaagg cttgctcgcc gaggcgcgcg gggcgctcga gcgcgcctcc
     9541 tcaggcttgc gcgaggagcg ctcctacgat ctcgtcgccg tggatctcga ggacgcgcgc
     9601 cgtgctctcg ccgaggttcg tgggcgcggc gtggacgacc aggtcgtcgc tgcgatcttc
     9661 tcccggttct gcatcggcaa gtgatgcagg gcggcccgag cgaagtggcg aagtccttcc
     9721 cactcttggt gatcggtgca ggtcatgccg gctgcgaggc cgcgtccatc gcggcccgcc
     9781 tcgggattcg ggtcggcctc gtcacgatgt cgctcgacgc gatcgcccac atgccctgca
     9841 atcccgcgat cggcggcctc gccaaggggc acctggtgcg cgagatcgat gcactgggcg
     9901 gattgatggg acgcatggcg gaccaggccg ggatccagtt caagctcctg aatcgcggcc
     9961 gaggccccgc ggtctggtcg ccgagagctc aggaggacaa ggctcgctat cgagcgctcg
    10021 tgcgcgcaca tctggaatcg acgccggaga tcgaggtcgt gcaggagcag gtggtcgcgc
    10081 tcgaggtcgc gggtgggcgc gtgcgcgggg tgcgtctggc gaacggcgcc gtgatcgggg
    10141 ccgagcgcgt gatcgtgact ccgggcacgt tcttgaatgg gctcatgcac gtcggcgagc
    10201 gcacgatcgc cgggggccga gtgggcgagc gcgcggcgcg cggaatctcg gactgcttga
    10261 ccgatctggg attccgcctc gtgcggctca agaccggcac gccgccgagg attcatcgcg
    10321 actcgatcga cttcgatcgc atgctgccgc agcgcggcga cgatcctccc cgtccgttct
    10381 cgcacttcac cgacgagctg gtcgtggacc agatcgagtg ccacctgacg ggaacgaccg
    10441 acgccacgca ccgcgccgtg cgtgagaatc tgcagcactc tccgctctac agcggccgca
    10501 ttcgaggcat cgggccccgg tactgcccgt cgatcgagga caaggtcgtg cgcttcgcgg
    10561 acaagcccac gcatcagatc ttcatcgagc ccgaggggcg cgacgtcgac gacttctaca
    10621 tcaacggcct ttccacgagc ctgcccgaag agatccagct ccagatcctc gcgaccatcc
    10681 ccggcctcga gcatgcgcgc atgatccggc cgggctacgc ggtcgagtac gacttcatcc
    10741 cgcccacgca gctccatccg acgctcgaaa cgcgcctcgt ccgcgggctc tacctcgcgg
    10801 gacagatcaa cggcacgtcg ggctacgagg aggcggccgc gcaaggactg atggcgggca
    10861 tcaatgcctc gctgtcgttg atggagcgcg agccgatggt gttgcgacgc gacgaggcct
    10921 acatcggcgt gctccttgac gatctcgtga ccaaggggac cgaggagcct taccgcatgt
    10981 tcacgtcgag cgccgagttc cgactgttgc ttcggcagga caatgcgtcg gagcgattgc
    11041 tgggtcgggc acgggagctt ggtgcgctgc gggatgagga ggtcgagcaa ttggaggatt
    11101 cggtgaggcg tcgcgtcgcg gcggcggagc ggatgcgaac gatgaaggtg gcgtgcgtcg
    11161 cgcatgccgc gacggcggcc gcggtggcga gtgtgggtgc ggcggcgagt ctggccgcgg
    11221 cggcgagcgc ggccccgacg gcgagcgcgc gagcggtctc gcccgaggag ctggtttcga
    11281 tcgcgaccgc gctgcgcgac ggccgcatga gcgtcgagga gtcctgtgct cgtcccgagc
    11341 ttgccgatct ggggaccgaa gccgtagagt ccgccgcgat cgagatccga tacgaaggct
    11401 acatcgcgcg ccagaagcgc gaggtggagc gcgcgcggcg cggtgagcaa ctggcattgc
    11461 cggactgcgt gttcgacgag ccgctcaacc agatgtcgcg ggagggccgt gagaagctgc
    11521 gccgtgtgcg accgtcgacc ctcgcgcaag cgtcgcgcat cccgggcatg tctccggccg
    11581 acatgtcggt gctcctgatc tatgccgagc gcgagcggcg gcgcgcgggg ctccgtcccg
    11641 cctgatctcg agtcggactt cgcagcgttc cgccgcgcgg tccagccctg gctcacatcg
    11701 gtgcgcggtg catccgcgga tggcgagctt gtcgaaagcc tcaagaagtt cggcctcctc
    11761 gtccacgagc gcgcggccag tctctccctg atcgcccaag gcgatcgccc cgttctcttc
    11821 acccgccaca tcctcgactc gctcaacctt gccaccatca cggaggcgcc cccggcatcc
    11881 ctcctcgacg tgggaagcgg cgccggcttt cctgggatcc cgctggccat cctctggccc
    11941 gagacccggg tcaccctgct cgagtcacgc gatcggaaag cgggctttct cgagctggcg
    12001 gtgcggcacc tcggcctgcg tcacgtccga gtggtctgcg atcgcctgga gcgcgtgagt
    12061 caggaccggt cgatcgatcc ggtcggcgct gtgaccgtgc gggcggtcgg cgacttgccg
    12121 cggctcctga ccctcgctgc gcccctggcc acggcggacg catggtgggc gtacttcgtt
    12181 gggggcgggc ttgcagagga cgatgcgagg acccttgctc cggaggcacg cgtcgagacg
    12241 ggcgcctttg gcgggaggat tctccgaggt tcctttcgcc gttcggactc gtaggaagcg
    12301 cgctgcaaag catgtttcac gtgaaacgtg gccacaagcc actgaaaaca cggcagctac
    12361 ccaggcgcct gcagacttga gccctcttcg agatccccgc cgttccgccc tcttgagtag
    12421 gcccaagtcc cagctcgcat cctgactcac ggccagggcc atggcgccga cctgtgcggc
    12481 gacacctcaa cgaccgcgac cgcgatgccc cgcgcgtcat gctccggcgc gcccccgcga
    12541 cgccaatccc gcccgcgaac gcctcaaacc tgcaccccgc tggacaacgc acgtattttc
    12601 agccacttcc cgccacgttt cacgtgaaac atctttcggt tggaggttcg ccacctcatc
    12661 gcgtagactg ccgacgcccc gacagaactg agccagcctc aggctcgatt gggtcaggga
    12721 aaccaacatg accaggacgg tcgcgatagc gaatcagaag ggtggggtgg ggaagaccac
    12781 gaccgcggtc aatctggcgg cggcgctggc gctcgcccag aagaaggtcc tactcgtcga
    12841 tctcgatccc cagggaaatg ccgggagcgg cctcggccat ccggccgtca aggtgggccg
    12901 cgggatctac gaggtcctga cgaccgacct ccccatccag gacgtgcttc tcgaaacgga
    12961 agtgcccggg ctcagccttg tcccgtcggg ccagcgtctc gctgccgctg aagtcgagct
    13021 ggtcgacgtg gagcggagag agttccgact caaagattcg ctgtcgaaga tcgaggaccg
    13081 attcgacttc atcatcatcg acgcgccccc gtcgctcggc tttctcacgg tgaatgcgct
    13141 cacgggcgcg aagtcggtgc tcgtcccggt gcagtgcgag tactacgcgc tggagggcct
    13201 gacgcacctc ctcggcgcca tccggctcgt gcagcagagt ctcaatccaa cgctccagat
    13261 cgagggcgtc cttctgacga tgtacgacaa tcgcctcaac ctctcgcagc aggtgctcga
    13321 ggagacgagg cgcttctttg cggagcgggt ctatcgaacc gtcatcccgc gcaacgtgcg
    13381 gctgagcgag gctccgagct tcgggaagcc cgtggtcatc tacgatccgg gatgcgttgg
    13441 gtcgacgagc tacatcaatt tggcgaggga ggtggtcgag cgtggctagg aaggcgttgg
    13501 gtcgtgggct cgaggctctg attccgacgg gggtcgatcg agaggttccg attgctccgc
    13561 catcgatggt cccgggcgcc ctaacctcgg agcctcggga gggcgacatt cagcacgtgt
    13621 ccctcacgaa gatcgtccgg aatccggtcc agcctcggac ccagttcgat ccggcgacca
    13681 tcaaggagct cgcggactcg atccgcgaac ggggcgtgct ccagccggtc cttcttcgtc
    13741 cggccgggga gggctatcag atcgtcgcgg gcgaacgccg attcctcgcg gcacgcgagg
    13801 ccggcttcac cacgattccg tcgatcgtcc gcccgctcac cgatcgcgaa tccctgctca
    13861 tcgccctcat cgagaacatc cagcgggaga acctcaatcc catcgacgaa gcgcgtgctt
    13921 attatcgcct cgcaacggaa tacgggctcc agcacgacga gatcggggct cgcgtcggca
    13981 aggatcgctc gacggtttcc aatctcattc gtttcaacaa cttgccagat acggtccaat
    14041 ccctcctcga atccggcagc atctcgggtg gccacgcgcg cgccctgctc ggcctctcgg
    14101 acgccaagag ccaggtcgac ctcgccaact ccgccgcgtc gcacgggtgg tcggtgcgcg
    14161 agctcgaaga tcgagtgaag cgttccgggc ccgcccgggc ccctcgcccg cggcgcgccc
    14221 ccaagcgcgc attctccggg gaactcatgg ccgtcgaaga tgagttgatg agggtgatgg
    14281 gggcgaaggt caggatcacc cgccgccggg gcggcatggg atcgatcctc atcgactacc
    14341 attcggagga ggagctggag cggctgatcg ggctcttccg ggacctcgcc gccacgcccc
    14401 gttgaataga tgtggataag tggcattgag cggtggaaaa ccggataaac tgattcccgg
    14461 atgaggattg cgcagctaag tacgatggac aagcggtcgg ttatgcacag ccgctgactt
    14521 atcgcatacg gacgcggaaa aacccgcggt catcccaacc caaaggacct ggacccatgg
    14581 cacaacccgt cgccacgagc gatgtgcagc tccttgaaga actgaagaac gcccgcgggg
    14641 agatcctggg cgagctccgg aaggtcatcg tcggccagga cgccgtcgtg gagcagctcc
    14701 tcacggtgct cttcgccaac ggccactgcc tcctggtggg cgtccccggt ctcgccaaga
    14761 cgctgctcgt ctccacgctc gcgacgatcc tcgatctcaa gttcagccgc atccagttca
    14821 cgcccgatct catgccgtcg gacatcaccg gcaccgagat cctcgaggac gacccggtgt
    14881 ccggaaagcg gacgttcaag ttcatccgag ggccgatctt cgcgaacatg gtgctcgcgg
    14941 acgagatcaa ccgcacgccg ccgaagacgc aggccgcgct tctccaatcc atgcaggagt
    15001 accgcgtgac ggccggcggc acgacgtatc cgctcgacct gccgttcttc gtgctcgcga
    15061 cacagaatcc gatcgagttc gaggggacgt acccgctccc cgaagcgcag ctcgaccggt
    15121 tcatgtacca catctccgtg ggctatccga ccgacgcgga ggaggagaag atcgtcgcga
    15181 ccacgacgag cgcgtacgaa gcgtcgccga agaaggtgat gggggtgcag cgcatcctcg
    15241 acctccagca cctggtccgg cgcattcctg tctccgagca cgtggtgcgg tacgccgtgc
    15301 gcctcgcgcg cgcgtcgcgc cccgggccgg agagcgcgtc cttcgttcgc gactgggtga
    15361 gctggggcgc ggggccgcgc gccgcgcaat acctcgtgct cggcgccaag gcgcgcgcag
    15421 cgatccaggg gcgccccacg ccgggcgccg aggacgtgcg ctcggtggcg ccggcggtgt
    15481 tacgccaccg catcatcccg aacttcaacg ccgaggcgga cggcatctcg agcctcgaca
    15541 tcatcgccaa gctcctcgag accacgccga gcgtcgacgg caagcacgcg tagcgggggc
    15601 gttccatgag cgagcgacgt ccccgactgc tggatccgca cttcgtctcg aagctgacgc
    15661 ggctcgatct caccgcgcgg ctcgtggtcg aaggattcct caccggcctc caccggagcc
    15721 cgtaccacgg cttcagcgtg gagttcgcgg agcatcgcca gtacatgccg ggcgatccac
    15781 tgcggcatct cgactggcgc gtgctggcca agtcggaccg caagttcatc aagcagtacg
    15841 aagaagagac gaacctccgc gcgatgctcc tcgtcgacac gagcgcgagc atgggctacg
    15901 gctcgcacgg cgtgaccaag ctcgactacg cgcgccagct cgcggcggcc ctcgcctacc
    15961 tcatgctgcg ccagaacgac gccgtcggca tgttcgcgtt cgcgaccggg cgcgcggagc
    16021 tgatcccgcc gcgatccacg ctagggcacg cgcggccgct cctcctcctg ctcgagcggc
    16081 tcacgccggg gggcgcgacc gacttcgctt cgtcgctcca ctcgctcgcc gagcggatga
    16141 accgccgggg actcgtggtt ctgatctcgg atctcctcga cgatccagag cggatcgccc
    16201 aggccatcca ccatttcagg cacaagaagc acgaggtcct cgtgttccac gtgctcgacc
    16261 cgcaggaagt ggcgttcgac ttcgagcgcg aggccgtgta cgtcgacctc gagacgggcg
    16321 agcgggtcac gacgcggccc caggagctcc gccccgacta ccgggcgcgc gtgagcgcct
    16381 ggcgcgacca gatccgccag ttctgcatcg agaagcgcgc cgagtacgtg cctctgacca
    16441 ccgaccagcc gttcgaccgg gcgctcctcg agtacctctc caagcgcgcg cggctcctct
    16501 agcgccaggc ccggatgccc gtcttctcgt tcctgaactc aggtttcctc gcggcgcttc
    16561 cgctcgcctc gatcccggtg atcattcacc tcctgagccg gcggcggctt cccgaggtcc
    16621 cgttcccgac gacgcagttc ctgcgccagc tcgagccgcg cgagatccgc cgccttcggc
    16681 tgcgcgaaat cctgctcctc atcctccgca cgctcgcgat cattctcctc gtcctcgcct
    16741 tcgcgcggcc gtccatcacg ccgcggaacg cggtgacgac cgccgcggcc gcgatggcgg
    16801 tgatcatcga cgactcggag agcatggcgg ggctcgacga gcaggcgaag ccgcgcatgg
    16861 atggggcgat cgagcgggcg cgcgcgctgg tcctcgccgc gcgccccggc gacgagatga
    16921 ccctgatctc ggcgacgcgc ccgatcggcg ccgcgaccga gcacacctcg gatcgcgtcc
    16981 ggctcggccg ttctctcgcg aacacacagg tcacgatgct cccggccgac atggcgggcg
    17041 cgctcctcat ggcgcggcgc acgctcgagc gaagccggct tcgggatcgc gagctctaca
    17101 tcatcagcga cctccagcgc tcgaacttcg acgccaaggc gcgcgcggag ctcggtccca
    17161 tcgcggccgc caagatccgc gtctacctcg tgcccgtcgt ccagtcgcgc gtgcccaacc
    17221 acgcgctcgt cgatctcgac ccggtgctgc gtcccggccc gatggggcgg ggtctcgagc
    17281 tgcgctcgcg catcgcgaac tacgcggacg cgccgagcga ccggatcgcg atccgcgtgc
    17341 gccacgggga cgcgctcgtg ggcgggggcg acgtgacgct caaggcggac gagatccgct
    17401 gggcggcgat gccgctcgac tggagaggtg cggggttcga ctcgtcccaa tccagcggcg
    17461 cagtcctgat cgaagcggac caggacgcgc tcgcgccgga cgatcgatgg ttcgcggtgc
    17521 tcggcgcgcc cggacgcctg cgcgtgcttc gcgtgaccga gccgcgcgag ggcgcggcgc
    17581 cgccgcgctt cgcgtcgctc gcgctcgatc ccgggaagga cgggcggagc gggttcgtcg
    17641 tcgacgaggt gtcgccggcc gggctcctcg gtctcacgaa gtcgcgctac gacgtggtgc
    17701 tgatcgagga cgtcgcgtcg ctttccaccg aaggcgaggc gcgcgtgcgc gcgttcctcc
    17761 gtgacggcgg cgggctcgtg gtcgcgctcg gaccgcacag cgatcccggc tactacggct
    17821 cgcgcctctt tcccggcgtc atcgatctcg gattggacgg accggagcaa gcggcggcgg
    17881 gaggctcgta cgagctcagg gcgaggctcc cgggacatga cgtgctgaac ggactcaacg
    17941 tcggggtcgg cgcaccgctc acggaatctc gcctcaccgg cctcatgaaa gcccgcacgc
    18001 tcaccacgcg ccccgaggtc gtggtgcaga cgacgggcgg gttgccgctc gtcgccacgt
    18061 cccccggcgt cgccgtgttc ctgagctcgc tctcggacga ttggggcgat ctcccgtatt
    18121 cgggagcgtt cgtccctctc gtccgcggcc tcgtctccta cgcggcgcag gccgacgcgc
    18181 agggccccgg gtccacgcgt gtcggggaag ctcccgcggc gcggctcacg tcgcctcccg
    18241 gcgcggcggt catcgtgcgc ggtcccgaga gctacagctc acaggcatcg gtgcgcgcgg
    18301 aaggcaccgc ctaccgcgcc gaagccgatg ctcccgcggt caagccaggg ttctacgact
    18361 tcgaagcggg cggccgatcg atcgcgaccg tggccgtcaa cgtgtctccg gtcgagagcg
    18421 atctcgccgt catcgacgcc gattcgctcc gcgcggccag cggcagcggc tcgtcgacct
    18481 tcaggagcgc gaacgcgctc gcgacccact tgagggacac gcgccgcggg aaggagctct
    18541 ggatgacctt cctcctcctc gccgcggccg ccctcgcggg tgagctcctc ctcggaagcg
    18601 cccgcgcgct ccgcgcatga gcgcgcccgg cccgctcccg cggcgcctcg tcgaggtcgc
    18661 cgcccgccat cccgcgctcg ccgcgctcgc gtcccgcgtc ggtgctccgt ccgggcgtcc
    18721 cgcgcacgtg cgcatccgcg gcgcctcggg gagctcgggg gcgctcacct tggccgcgct
    18781 cctcgaggga agcacgcgtc ccatggcggt gatcgcgccc gacatcgatc gcgccgaggc
    18841 gtggcgcgac gatctcgaat tcctgctcgg cgaggagcgc gtggtctatc tcccgccgct
    18901 cgatgtcgtc gcctggagcc atcagctcgc gaccgccccc gtacgggacg accggctcca
    18961 ggccatcgtg cgcctgggcg atcccgatcc cccggtcgcg gtgctcccgg cggccgcgct
    19021 ccaccgcctc gtgcccaagc cggaccgcgt gcgcgcccgc tcgatcacgc tcacgagtgg
    19081 atacgaggcc gatcccgagg gtctcgtccg tcgtctcgtg gtcggcggat accgggccgt
    19141 ggccgagatc ggcgaatcgg g
//
LOCUS       DASTKC010000031        10718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_968859, whole genome shotgun sequence.
ACCESSION   DASTKC010000031 DASTKC010000000
VERSION     DASTKC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968859"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFR25_01135"
     CDS             complement(<1..679)
                     /locus_tag="VFR25_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9939683.1"
                     /translation="MNDPSAAPLALEATGLAKTYQEGLTPVEVLRGVDLVVEPGEMVA
                     VVGASGSGKSTLLHLLGGLDAPSAGQVRVGGEPMTALNEEGRSRLRSRAVGFVFQFSQ
                     LLPEFTALENAMMPGWIAGGRAEAEARGRAEELLRDLGVMERAEHKPSELSGGEQQRV
                     AMARALHRRPAVLLADEPTGNLDRNAAQGLVEVLQRYRRDHGQATVIVTHNPDLARSA
                     DRVVLLEE"
     gene            complement(672..1892)
                     /locus_tag="VFR25_01140"
     CDS             complement(672..1892)
                     /locus_tag="VFR25_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9939684.1"
                     /translation="MSYVWIIASRYLRSKRRLNFITLVSLLAVGGVLVGVAALTIVLS
                     VMNGFEDQVQQRIAGTNAHVAVLSGDDAPIPQDPALERAILGAVPDAHVAPFVYGKVM
                     VASRHGIDGMVLKGVDPDQERGVTDILARLTPPVGSFDGGDLPGIALGQELASRLRVI
                     KGDVILISVPSDEGGLLGGTPRSMRLRVSSIFRSGLYEYDSSFGVVPMSTAQGFFGLD
                     TDVTGYQLRIPDMFRAQEISRRLDAALGPGYRTTNWIDLNRNLFAWMKIEKAVMFTIL
                     ILIVIVAAVNIVSSLVMLVLEKRRDIGVLRTMGVTPRGIMRIFLLQGTLIGLSGTTLG
                     LLLGSAVSFALGRYKLLHLPGEIYFIDTLPVKMEWPDFVFVAVAATLTCFLASLYPAW
                     QAARLAPVQTIRYE"
     gene            complement(1921..3384)
                     /gene="lysS"
                     /locus_tag="VFR25_01145"
     CDS             complement(1921..3384)
                     /gene="lysS"
                     /locus_tag="VFR25_01145"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9939685.1"
                     /translation="MSVQETRREKLEALTARGIQPYAYAFPVTHRSALIRSEAARLIE
                     SAETVRIAGRLMTRRGHGKACFAHLKDADGTIQIYLREDVLGPEAYENALALDLGDWV
                     GVTGVVFTTKTGETTVRADRIELLSKSLTPLPEKWHGLTDVETRYRQRYADLAVNDDV
                     RAVFRARAAIVRALREFLDARGFLEVETPVLQPLYGGATARPFVTHHNALDRKLYLRI
                     AVELYLKRLIVGGLDRVYEIAKTFRNEGIDRFHNPEFTMLEFYQAYADYEEMMQVTED
                     LLLHTVRSVHGGLQLEFDGKTIDFAPPWPRISFAEAARRGLGVDRLPEDEAGLRALAE
                     RAKIPVDPRVTYGKILDEIVSATYQPGITSPTFLIDHPRDISPLAKVKRGDPSVVERF
                     ELIVAGMEVANAFSEQNDPAEQRRAFEVQLEQRKRGDDEAQLLDADYLRALELGMPPT
                     AGCGIGVDRLVMFLTDSRSIRDVVLFPQMRPEEGEEG"
     gene            complement(3487..5520)
                     /locus_tag="VFR25_01150"
     CDS             complement(3487..5520)
                     /locus_tag="VFR25_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9939686.1"
                     /translation="MTDEESAKENKNRKKLGEYLLEAGLLTEPQLKEALRRQKQSKEP
                     LGRILARTGVVSETDVCRILHQQLGLPLVDLSTVAIDDAVIGLVREDLAKKYTAIPLD
                     LEARNTLRIAMADPLNAAALEDLRFQSGHFIRPVLAPPSAIVDAISKYYHIDANLTGI
                     LETIIQNEPTSELKEIVDSDADVSVDELMKISSGPPIVRLANWLLTRAVEMRASDIHV
                     EPQDKMVGVRVRVDGLLQDLERLPKWTQGALTSRLKILATLDIAEKRLPQDGRFRVEV
                     DGRRIDLRVSTLPTHHGEKVVIRIADQERSAFRIDSLGLSERDIERMRAYGTRPQGIL
                     IVTGPTGSGKTTMLYSLLQQIHSVTKNIVTVEDPIEFQVPGVNQVQVDEKSKKTFAAI
                     LRAMLRQDPDVMMIGEIRDLETAQIAFRASITGHLVLSTVHTNDAPSAITRLIDLGLQ
                     PFMVASSLIGVVSMRLVRTLCPKCKDTYGPTGDELRLLGLSPRDAGDLALHRPVGCEF
                     CGESGYRGRTGVFEVLDMTDPIRRLIASGASESMIRQAAIEAGMVPIGQDGLAKVIAG
                     ESSLEELRRVLYYEEEMARLCQSCHRSVSAEFLYCPHCGGPSSTVCVGCHRRIDPQWL
                     FCPSCGHECTAAQRPVVEPLVPADPQVPTDPDDASLWEELAEGRRAKDGRRDA"
     gene            complement(5806..7287)
                     /gene="purF"
                     /locus_tag="VFR25_01155"
     CDS             complement(5806..7287)
                     /gene="purF"
                     /locus_tag="VFR25_01155"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET9939687.1"
                     /translation="MKERSSVASDTVQGTSIEVAARNQDRLREECGVFGIWGAENAAR
                     LAYAGLYALQHRGQESAGIVATDGVEYREHKGLGLVSDVFAGRALDTMRGSIAIGHNR
                     YSTTGGSQLANAQPLVVNYREGKIAIGHNGNLVNALSLRDEMEGKGSIFQTTSDTEVI
                     LHLIARSSATEIERMIPEALGRCQGAYSLVILAGDKLIGVRDPRGFRPLCLGRRGEAH
                     VLASETCALDIVGADFVREIEPGEMVVIDGSGVKSYRLFRPEPQRACVFELIYFSRPD
                     SVVFGASVDSIRRRLGRRLSEEHPAEADIVISVPDSSNSAALGFSEQSGIPFELGLIR
                     NHYVGRTFIAPHQITRDFGVNLKFNAVRRILDGKRVVVVDDSIVRGTTSRSLVAMLRA
                     AGAREIHFRVSSPPIGWSCYYGIDTPNRRELIASSHTVEQIRKYLHVDTLGYLSMEGL
                     RACVDRPDDHCYACFDGDYSVRFGEEMDKLALERPRAKTAEAP"
     gene            complement(7284..7886)
                     /locus_tag="VFR25_01160"
     CDS             complement(7284..7886)
                     /locus_tag="VFR25_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939688.1"
                     /translation="MSAVLRTRLKISDLTAWTALDAARKLLPEGHTLQKLVREEIFVF
                     EPAEGSRAPEFESELAHAIESSNFFVNPNKEQYRFTRAAERGASLAPPEGAWGILARP
                     RGDTQDEGLLERLLREHPMKGIGAIRRARLWWLWTVGSGGGADDRDACYRAVGPVMGS
                     RRGLLVNPHSETDLRIDGPTAWDAVEAFLRAPAASRREAA"
     gene            complement(7883..8719)
                     /gene="purQ"
                     /locus_tag="VFR25_01165"
     CDS             complement(7883..8719)
                     /gene="purQ"
                     /locus_tag="VFR25_01165"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HET9939689.1"
                     /translation="MAHVAVLQIPGVNCEYETVRVLEAVGLEAAIVRFNEPSSSLAAF
                     DAFVLPGGFAYQDRIRAGAVAAKLSAVERIAQESESGKPVLGVCNGAQVLVEAGLVPG
                     LHKGAVELALAPNRAPRRQGYLCRWVRVRAGEGPGAALWTSALQGDRMAPLPMAHGEG
                     RFDTADPAVRDRIERDGLALFRYVDAEGQPASRYPDDPNGAMLQAAGITNVRGNVLAI
                     MPHPERASWLFQVPLDWRGAWGDRRRAAVGSRSALDGAGPGRFLFESLARHLGVAKRE
                     AA"
     gene            complement(8722..>10718)
                     /gene="purL"
                     /locus_tag="VFR25_01170"
     CDS             complement(8722..>10718)
                     /gene="purL"
                     /locus_tag="VFR25_01170"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET9939690.1"
                     /translation="GAATGIGGIVRDVNCMGAEVIGVLDALRFGDPEGPRASGVREIV
                     RGVVDGIWQYGNPLGVPNLGGDVFFSDRYDENCLVNVVALGLVREDRVVRSRVPEAAS
                     REPYVLVLVGKPTDETGFGGASFASAILEQDAPEQRGHVQVPDPFLKRVLFEANRAAL
                     DWLFREGAPFGFKDLGAGGIACASSELAAAGGFGMDVDLDLVPTPRGEYPPEVIACSE
                     TQERFALVVPERLAPKLLAIYNEDYALPGVYDGARAAVIGRVRKDPSYRILRHGQAVC
                     EAPVEVITAGIEHRRAERPRATAPSTAPELHVPDHGAFLRRMIASPNLGSRDPILRYY
                     DTEVQARMAVRAGEADASVLVPVPGAPLGAAITVDGNPWYVAADPYWGAAHVVYEALR
                     NLVAVGARPIALTDCLNFGNPEDPEVFDEFVRSVRGLGDAARAFGPNGEAGPPVPIVS
                     GNVSFYNESSTGRSIEPSPIVAALGVLDDYSVAVSSGLKKAGSTLFLTGRRQSSMGAS
                     QAQYLLAGEKGGALPSLDFEMERRRLHVVLEAVRRGLVLACHDVSDGGVAMAAFEMAL
                     GGWSAMGLGLELRVPDADGIAIEARLYGETPGFLLEIASGREVEVGALFQKAGVDLTA
                     VGSTMAEPRLRVVSQGSTLVDADLAELARIHSDAIRPYVE"
BASE COUNT         1727 a         3721 c         3629 g         1641 t
ORIGIN      
        1 cctcctcaag caggacgacg cgatcggcgg aacgggccaa atccgggtta tgtgtcacga
       61 ttacagtcgc ttgcccgtga tccctccgat accgttggag cacctcgacc agcccctggg
      121 ccgcgtttcg gtccagattc ccggtcggtt cgtcggccag gaggaccgcc ggtcttcgat
      181 gcaaagcgcg ggccatggcc accctctgct gctccccacc cgaaagctcc gacggcttgt
      241 gctccgcccg ctccatgact ccgagatctc ggaggagctc ctcggcgcga ccgcgggcct
      301 ccgcctcagc ccgcccgccc gcgatccagc ccggcatcat cgcgttttcg agcgcggtga
      361 actccgggag gagctgcgag aactggaaca caaagcctac cgcgcgggag cggaggcggg
      421 agcgcccttc ttcgttgagc gcagtcatcg gctcgccgcc gacccgaacc tggccggctg
      481 acggcgcgtc cagtccgccg agcaggtgaa ggagagtcga cttgccggag cccgacgcac
      541 ccaccaccgc caccatctcc cccggctcga ccacgaggtc cacgccccgc agcacctcga
      601 cgggggtcag gccttcctgg tacgtcttcg cgagtccggt cgcctcgagg gcgagcgggg
      661 ccgcggaggg gtcattcata gcggatcgtc tggaccggag caagcctggc ggcctgccac
      721 gctggataaa gactggccag gaagcacgtg agggtggcgg ccacggcgac gaacacgaaa
      781 tcgggccact ccattttgac cggcaaggta tcgatgaagt agatctcccc cggcaggtgg
      841 aggagcttgt agcggcccag ggcgaaggag accgcggacc ccaggaggag ccccagcgtc
      901 gttcccgaca ggccgatgag cgtcccttgc aggaggaaga ttcgcatgat tccgcgaggg
      961 gtgaccccca tggtgcggag taccccgatg tcccgccgct tctccagcac cagcatgacc
     1021 aggctcgaga cgatgttcac cgccgccacg atcacgatca ggatgaggat cgtgaacatg
     1081 accgccttct cgattttcat ccaggcgaag agattccggt tcaggtcgat ccagttggtc
     1141 gtgcggtacc cggggccgag cgccgcgtcc agccggcgcg agatctcctg cgcccggaac
     1201 atgtcgggaa tgcggagctg gtagccggtc acgtccgtgt cgagtccgaa gaatccctgc
     1261 gcggtgctca tcggaacgac gccgaacgac gagtcgtact cgtagaggcc tgagcggaag
     1321 atcgagctga cccggagcct catggatcgc ggcgtgccgc ccaggagtcc gccctcgtcg
     1381 ctcgggacgc tgatcaggat gacgtctccc ttgatgacac gcagacgcga ggcgagctcc
     1441 tgtccgagcg cgatcccggg aaggtcgccg ccgtcgaagg aaccgaccgg cggtgtgagc
     1501 cgcgccagga tgtcggtgac gccgcgctcc tggtcgggat ccacgccctt cagcaccatg
     1561 ccgtcgatgc cgtggcgcga cgccaccatg accttcccgt acacgaaggg agcgacgtgc
     1621 gcgtcgggca ccgcgcctag gatcgcgcgc tcgagcgccg gatcttgcgg gatcggcgcg
     1681 tcgtcccccg acaggaccgc gacgtgcgcg ttcgtccccg cgatccgctg ctgcacctga
     1741 tcctcgaagc cgttcatcac cgagaggacg atggtgaggg ccgcgacgcc gaccagaacg
     1801 ccccccacgg cgagaaggct cacgagcgtg atgaagttga ggcggcgctt ggaccggagg
     1861 tagcgcgagg cgatgatcca gacgtagctc atcagcgttc ccgacccgcg cggcgcggga
     1921 tcagccttcc tcgccttcct cgggccgcat ctgcgggaag agcaccacgt cacggatgga
     1981 gcgcgagtcg gtgaggaaca tcacgaggcg gtccacgccg atcccacatc cggcggtcgg
     2041 cggcatcccc agctcgagcg cgcggaggta gtcggcgtcg aggagctggg cttcgtcgtc
     2101 tccgcgcttc cgctgctcga gctgcacctc gaaggcgcgg cgctgctcgg ccgggtcgtt
     2161 ctgctcggag aacgcgttcg cgacctccat cccggcgacg atcagctcga accgctcgac
     2221 cacgctggga tcgccgcgct tcaccttggc gaggggcgag atgtcgcgcg gatgatcgat
     2281 gaggaacgtg ggcgaggtga tcccgggctg gtacgtggcc gagacgatct cgtccaggat
     2341 cttcccgtac gtgacgcgcg gatcgaccgg gatcttggcc cgctccgcaa gcgcgcgaag
     2401 gccggcttca tcctccggga gtcgatccac gccgaggccg cggcgcgccg cttcggcgaa
     2461 ggagatccgc ggccagggcg gcgcgaaatc gatcgtcttg ccgtcgaatt ccagctggag
     2521 cccgccgtgg accgagcgca ccgtatgcag gaggagatcc tcggtgacct gcatcatctc
     2581 ctcgtagtcc gcgtaggcct ggtagaactc gagcatcgtg aattccggat tgtggaaccg
     2641 gtcgatgccc tcgtttcgga acgtcttcgc gatctcgtag acgcggtcga gcccgcccac
     2701 gatgagccgc ttcaggtaga gctccaccgc gatgcggagg tagagcttcc ggtcgagcgc
     2761 gttgtgatgg gtgacgaacg gccgtgccgt ggcgccgccg tagagcggct ggagcacggg
     2821 cgtctccacc tcgaggaagc cgcgcgcatc aaggaactcg cggagcgccc gcacgatcgc
     2881 ggcgcgcgcg cggaagaccg cgcggacgtc gtcgttcacg gcgaggtccg cgtaccgctg
     2941 gcgatagcgc gtctcgacgt ccgtgaggcc gtgccacttc tcggggaggg gcgtgagcga
     3001 cttcgagagg agctcgatcc ggtccgcgcg cacggtggtc tcgccggtct tcgtcgtgaa
     3061 gaccaccccc gtcacgccga cccagtcccc cagatccagc gcgagcgcgt tctcgtacgc
     3121 ctcgggaccg aggacgtcct cgcgaaggta gatctggatc gttccgtccg cgtccttgag
     3181 gtgcgcgaag cacgccttgc cgtggccgcg ccgggtcatg aggcgccccg cgatccgcac
     3241 ggtctcggcc gattcgatga ggcgggcggc ctcgctccgg atgagcgcgg agcgatgggt
     3301 gaccgggaac gcgtacgcgt acggctgaat gcctcgggcc gtcagcgctt cgagcttctc
     3361 gcggcgggtt tcttggacgg acacggttcc ccgggaatca acggaaacgc gcaccggttg
     3421 acgcgcggcg cgcgcggcgg atcgaaggag agcggaggcg atggtacgta gaggccgcgg
     3481 ccccggtcaa gcgtcgcggc gaccgtcctt ggcacggcgt ccctcggcga gctcctccca
     3541 gagggaggcg tcgtcggggt cggtgggcac ctgcgggtcc gccggcacca gcggctcgac
     3601 gacgggcctc tgcgccgcgg tgcattcgtg gccgcacgac ggacagaaga gccactgcgg
     3661 atcgatgcgg cggtggcagc ccacgcaaac ggtcgacgac ggtccgccgc agtgcggaca
     3721 gtagaggaac tcggcggaga ccgagcggtg gcaggactgg cagaggcgcg ccatctcctc
     3781 ctcgtagtag aggacgcgcc gaagctcttc caggctcgac tcccccgcaa tcaccttcgc
     3841 gagaccgtcc tggccgatcg gaaccatccc cgcctcgatc gcagcctgcc ggatcatcga
     3901 ctccgacgcg ccgctcgcga tcaggcggcg gatcggatcg gtcatgtcga gcacctcgaa
     3961 cacgccggtc cggccgcggt agcccgactc gccgcagaac tcgcatccga ccggacggtg
     4021 gagtgccaag tcgcccgcgt cgcgcggaga gaggccgagc aaacggagct cgtcccctgt
     4081 cggaccgtag gtgtccttgc acttcgggca gagcgtccgg accaggcgca tggagacgac
     4141 accgatcaag ctcgacgcca ccatgaaggg ctgtagcccg agatcgatga gccgggtgat
     4201 ggccgacggg gcgtcgttcg tgtgcaccgt cgagagcacg aggtgccccg tgatcgaagc
     4261 gcggaaggcg atctgcgccg tctccaagtc gcggatctca ccgatcatca tgacgtccgg
     4321 gtcctgccgc agcatggcgc gcaagatcgc cgcgaacgtc ttcttgctct tctcgtcgac
     4381 ctggacctgg ttcacgcccg ggacctggaa ctcgatcgga tcctcgacgg tcacgatgtt
     4441 cttcgtcacc gagtggatct gctggaggag cgagtagagc atcgtcgtct tgccgctgcc
     4501 ggtgggtccc gtgacgatga ggattccctg cggtcgcgtg ccgtacgcac gcatccgctc
     4561 gatatcccgc tcgctcagac ctagggaatc gatccggaac gcggaccgct cctggtccgc
     4621 gattcggatc acgaccttct cgccgtggtg ggtcggcagg gtcgagacgc gaagatcgat
     4681 acggcggccg tccacctcga cccggaagcg accgtcctgc gggagccgct tctccgcgat
     4741 gtcgagcgtg gccaggatct tgaggcgcga cgtgagcgct ccctgggtcc acttagggag
     4801 ccgctccagg tcttggagca ggccgtccac gcgcacgcgc acgccgacca tcttgtcctg
     4861 cggctcgacg tgaatgtcgc tcgcccgcat ctcgacggcg cgcgtgagga gccagttggc
     4921 gagccgcacg atcggcgggc cggagctgat cttcatcagc tcgtccaccg acacgtcggc
     4981 gtcgctgtcg acgatctcct tcagctcgga ggtcggttcg ttctggatga tggtctcgag
     5041 gatgcccgtg aggttcgcgt cgatgtggta gtacttcgag atcgcgtcca cgatcgccga
     5101 cggcggcgcg agcacgggac ggatgaagtg gccgctctgg aagcgaaggt cctcgagcgc
     5161 ggcggcgttc agaggatcgg ccatggcgat gcgcagcgtg ttccgggcct cgagatcgag
     5221 cgggatggcc gtgtacttct ttgccaggtc ctcccgcacg agcccgatca cggcgtcgtc
     5281 gatcgccacc gtggagagat cgacaagggg aagcccgagc tgctggtgga ggatgcggca
     5341 gacgtccgtc tcgctcacga ctcccgtgcg agcgaggatc cgcccgagcg gctccttgct
     5401 ctgcttctgg cgccggagcg cctccttgag ctgcggctcc gtgagaagcc cagcctcgag
     5461 gagatactcc cccagcttct tccgattctt gttttctttc gcgctttctt cgtcggtcac
     5521 gagcggcccc cgtgcatccg tgcaagcggc ggagcggatg aagccgttcc gtgatgggca
     5581 aagcccgtgc cacgaggggg aaaggaccgg agcgcccgtg ccgagcgcga ggatcaagcg
     5641 gttaggggcg tcgggagcgc acggtcgtgc gggtcaggcg tgcgatttcg cggagacgat
     5701 gcactctgca cggtcgggta cgcggccgat gatgccttcg gccggtgcaa caggtcgtgc
     5761 ggcatggctc gtccccgacc ccggcctcag aacctcagca acggctcagg gggcttcagc
     5821 agtcttggcg cgaggccgct ccaaagcgag cttgtccatc tcctcgccga accgcaccga
     5881 atagtcgccg tcgaagcacg cgtagcagtg gtcgtccggg cggtcgacgc aggcgcggag
     5941 cccttccatc gagaggtacc ccagcgtgtc gacgtgcagg tacttgcgga tctgctccac
     6001 cgtgtgggac gacgcgatca gctcgcgccg gttcggcgtg tcgatgccgt agtagcagga
     6061 ccagccgatc ggcgggctcg agactcggaa gtggatctcg cgcgcgccgg cggcgcggag
     6121 catggcgacg aggctccggc tcgtcgtgcc gcgcacgatc gagtcgtcca ccaccacgac
     6181 gcgctttccg tcgaggatgc ggcggacggc gttgaacttg aggttcacgc cgaagtcgcg
     6241 cgtgatctgg tgcggcgcga tgaaggtgcg cccgacgtag tggttccgga tgagccccag
     6301 ctcgaacgga atgcccgact gctcggagaa tccgagcgcg gccgagttgc tcgaatcggg
     6361 gaccgagatc acgatgtccg cctcggccgg atgctcctcg gacaggcgcc gcccgagccg
     6421 gcgccggatc gagtcgacgc tcgcgccgaa gaccacgctg tccggacgcg agaagtagat
     6481 cagctcgaac acgcaggcgc gctggggttc ggggcggaag agccggtacg acttcacccc
     6541 cgacccgtcg atcacgacca tctcgcccgg ctcgatctcg cggacgaagt ccgcgcccac
     6601 gatgtcgagc gcgcaggtct cgctcgcgag cacgtgcgcc tcgccgcgac ggcccaggca
     6661 gagcggacgg aagccgcgcg gatcgcgcac gccgatcagc ttgtccccgg cgaggatcac
     6721 gagcgagtag gcgccctggc agcgcccgag cgcctccggg atcatccgct cgatctcggt
     6781 cgccgaggag cgggcgatga gatgcaggat cacttcggtg tcgctcgtgg tctggaagat
     6841 ggagcccttc ccttccatct cgtcccggag cgacagcgcg ttcacgaggt ttccgttgtg
     6901 gccgatcgcg atcttgcctt cgcgatagtt cacgacgaga ggctgcgcgt tggcgagctg
     6961 gctcccgccg gtcgtggaat accggttgtg gccgatggcg atcgatccgc gcatcgtgtc
     7021 gagcgcacgg cccgcgaaga cgtcggagac gaggccgagc cccttgtgct cgcgatactc
     7081 gacgccgtcg gtcgcgacga tgcccgcgct ctcctgaccg cggtgctgaa gcgcgtagag
     7141 cccggcgtag gcgagccgcg ccgcgttctc cgccccccag atcccgaaca ccccgcactc
     7201 ctcccgcagg cggtcctgat tccgggccgc cacctcgatc gaggtcccct gcaccgtgtc
     7261 tgacgccacg ctcgaacgct ccttcatgcg gcctcccgcc gcgaggcggc cggcgcgcgg
     7321 aggaacgctt ccacagcgtc ccacgccgtc ggcccgtcga tcctgagatc cgtttccgaa
     7381 tgcggattca ccaacagtcc ccgtcgcgat cccatcaccg gcccgaccgc gcgatagcac
     7441 gcgtcgcgat cgtccgcgcc cccgcccgat ccgaccgtcc agagccacca gagtctcgcc
     7501 ctgcgaatcg cgccgattcc cttcatcgga tgctctcgaa gcagacgctc gagaagtccc
     7561 tcgtcctgcg tgtcgccgcg gggccgcgcc aggatacccc aagccccttc gggcggcgcg
     7621 agcgaagctc cgcgctccgc ggcgcgggtg aaccggtact gctccttgtt cggattcacg
     7681 aagaagttgc tgctctcgat cgcgtgcgcg agctcgcttt cgaactcagg cgcgcgcgat
     7741 ccctccgccg gctcgaagac gaagatctcc tcgcgaacca gcttctggag cgtgtgccct
     7801 tccgggagaa gcttccgcgc cgcgtcgagc gccgtccacg ccgtcagatc cgaaatcttg
     7861 agccgcgtcc ggaggaccgc gctcatgccg cctcccgctt cgccacgccg agatgccgcg
     7921 cgagcgactc gaacaggaag cgcccggggc ccgcgccgtc cagcgcgctt cgcgatccca
     7981 ccgcggcacg gcgccggtcg ccccacgcgc cgcgccagtc gagcggcacc tggaagagcc
     8041 aggacgcgcg ctccgggtgc ggcatgatcg cgagcacgtt gcctcgcacg ttcgtgattc
     8101 cagcggcctg gagcatggcg ccgttgggat cgtcgggata tcggctggcg ggctgcccct
     8161 ccgcgtccac ataacggaat agagccaacc catctctttc gatgcgatcg cgcacggcgg
     8221 ggtccgcggt gtcgaagcgc ccctcgccgt gcgccatcgg aagaggcgcc atgcgatcgc
     8281 cttggagcgc cgaggtccag agtgcggcgc ctgggccctc ccccgcgcgc acgcgcaccc
     8341 agcggcagag gtagccctgg cggcgcggcg cccggttggg cgcgagcgcg agctcgacgg
     8401 ctcccttgtg gaggccggga acgagcccag cctcgaccag cacctgcgct ccattacaaa
     8461 caccgaggac cggtttcccg ctttcggatt cctgtgcgat ccgctcgacc gcggacagct
     8521 tggccgcgac cgccccggcc cggatccgat cctgatacgc gaatccgccc ggaagcacga
     8581 atgcgtcgaa cgccgcgagc gatgacgacg gctcgttgaa ccgcacgatc gccgcctcga
     8641 gccccacggc ttccagcacg cgcacggtct cgtactcgca attgacgccc ggaatttgaa
     8701 ggacagccac gtgcgccatc gtcactccac gtaggggcgg atcgcgtcgg aatggatccg
     8761 cgcgagctcc gccaggtccg catcgacgag cgtcgagccc tgactcacga ctcgaaggcg
     8821 aggctcggcc atcgtactgc ccaccgcggt cagatcgacg ccggcctttt ggaagagcgc
     8881 gcccacctcg acctcgcgcc cggacgcgat ttcgaggagg aagccgggcg tctcgccata
     8941 caggcgcgcc tcgatcgcga ttccgtccgc gtcggggact cgaagctcga ggcccagccc
     9001 catcgcgctc caaccgccga gcgccatttc gaacgcggcc atcgcgaccc cgccgtccga
     9061 gacgtcgtgg cacgcgagca cgagaccgcg gcgcacggct tcaagtacta catgaaggcg
     9121 gcgacgctcc atctcgaaat cgagcgaggg aagcgcccca cccttctctc ccgcaagaag
     9181 atactgggcc tgggatgcgc ccatggacga ctggcggcgc ccggtgagga atagcgttga
     9241 gccggctttc ttgagtcccg agctcaccgc gacggagtag tcgtcgagca cgccgagcgc
     9301 cgccacgatg ggcgagggct cgatggagcg gccggtcgag gactcgttgt agaaggagac
     9361 gttccccgag acgatcggga ctggcggtcc cgcttccccg ttcggcccga acgcgcgcgc
     9421 cgcgtcgccc agcccgcgca cgctccggac gaactcgtcg aagacttcag ggtcctcggg
     9481 attgccgaag ttcaagcagt cggtcagcgc gatcggcctc gctccaacgg cgacgagatt
     9541 gcggagggcc tcgtacacga catgggccgc gccccagtac gggtccgcgg cgacgtacca
     9601 gggattgccg tcgaccgtga tcgccgcacc gagaggcgcg cccggcacgg ggacgagcac
     9661 cgacgcgtcc gcctcccccg cgcgcacggc catccgcgct tgcacctcgg tgtcgtagta
     9721 gcgaaggatc ggatcgcgcg agcccagatt gggggacgcg atcatccggc gtaggaacgc
     9781 gccgtggtcc gggacgtgga gctccggagc cgtggaaggc gccgtggcgc gcggacgctc
     9841 cgcgcggcgg tgctcgatcc cggccgtgat cacctcgacc ggcgcctcgc acacggcctg
     9901 accgtggcga aggatgcgat aggacggatc cttgcgaacc cggccgatca ccgcggcgcg
     9961 cgcgccgtcg tacacgcccg ggagcgcgta gtcctcgttg tagatcgcga ggagcttcgg
    10021 tgcgaggcgc tcgggcacga cgagcgcgaa gcgctcctgc gtctccgagc aggcgatcac
    10081 ctcgggcgga tactccccgc gcggcgtggg cacgaggtcg agatccacgt ccatgccgaa
    10141 tccaccggcc gccgcgagct ccgaggacgc gcacgcgatc ccgcccgcgc ccaggtcctt
    10201 gaagccgaac ggcgcccctt ctcggaagag ccaatcgagc gcggcgcgat tcgcctcgaa
    10261 gagcacgcgc ttgaggaacg gatccggcac ctgcacgtgc ccccgctgct ccggcgcgtc
    10321 ctgctcgagg atggccgacg cgaacgaggc gccgccgaat ccggtctcgt ccgtcggctt
    10381 gccgacgagc acgagcacat agggctcgcg ggaggcggcc tcggggacgc ggctccgcac
    10441 cacgcgatcc tcccggacca gaccgagcgc gaccacgttc acgaggcagt tctcgtcgta
    10501 gcgatccgag aagaagacat cgcccccaag gttcggaact ccgagcggat ttccgtactg
    10561 ccagatgccg tccacgacgc cccgcacgat ctcgcgcacg ccgctcgcgc gcggaccttc
    10621 gggatcgccg aagcggagcg cgtcgagaac gccgatcacc tccgccccca tgcagttcac
    10681 gtcgcgcacg atcccgccga tcccggtcgc ggcgccct
//
LOCUS       DASTKC010000032        10819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1679624, whole genome shotgun sequence.
ACCESSION   DASTKC010000032 DASTKC010000000
VERSION     DASTKC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679624"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VFR25_01175"
     CDS             <1..1294
                     /locus_tag="VFR25_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9939691.1"
                     /translation="LAELTSLFQLGGTLQMNLPLETVAEITVRRVASSLHSYDATLFL
                     IDAAGRRLVPRATFGLTARGPEPEAAVGEGALGWAARAREPILMRAADHEARFADFFT
                     KHPDAGSVLILPVCVEKRCIAVLQVCRASCADPFRIEHRDVGQLFADNVAPVIDRAQA
                     QLRFREAAAVAGTVTPPAEEIASGAFRDVFLTSAANELKSPLTSLVAYTEVFDQNDPA
                     MTPATRREFTARLYEEAQRMLGLVDDVLDLVRLELGRYLLDVRLGSVNQAVRAARDLV
                     APFAESRSVAIDLDLEGSVPDQHFDPAKIRQSLVHILRHAIRFSPQRSRVRLRSIVDG
                     EGVLIEVKDSGPTIPAEASEAMFNLESVGDQFGKRCKERQGFGLHLARRFVELHGGRV
                     GAGPAPDGGAVIWVRLPRGEDLSSLLGSAPFMEDFAKS"
     gene            complement(1302..2408)
                     /locus_tag="VFR25_01180"
     CDS             complement(1302..2408)
                     /locus_tag="VFR25_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9939692.1"
                     /translation="MRWSWKVATLAGIPLRVHATFFLIFPYAWFAEAQGDPKRGVEAI
                     ALIIALFTCLVLHELGHALTARRFGVRTRDITLLPIGGVARLERIPDKPVQEIAVALA
                     GPAVNVVIASILLAIIAATGNLATFATADALEHNFVTKLLSMNVLLVIFNMIPAFPMD
                     GGRVLRALLAIRLDYVKATRIAASIGQGIAILFGLLGLIWNPMLLFIAMFVFLGAGQE
                     SAAVQMRSAFDGVPVWKAMIRDFRALQGDEPVSRAVELLLNGHQQDFPVIGLPGDPPV
                     GILLRADLFRALSEGKTNVRVDEIARRTCGSAHPREMLEGAVLRMQERGCPALPVLEE
                     DGALVGMLTLENVGELATVQAALGSAARSRRAVS"
     gene            complement(2417..3157)
                     /locus_tag="VFR25_01185"
     CDS             complement(2417..3157)
                     /locus_tag="VFR25_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939693.1"
                     /translation="MLDKRMMRILSGMGAALVLAFVLAGCGDQQKAVADKEQPKEQGA
                     TTTEDKLAPAGEEQTAEQTPQKSGPTSHTYTLATGTSFSGTLQQTLSSGKNHVGDPFS
                     IRTTLAKTVDGATAVPAGSTIRGHLSYVDGAGRVAGGAKLTLRYDSIKLPDGTTYDIS
                     AVPLRLEGKGDAKESALEIGGGAVAGGIIGGVLGHGSGDVAKGAAVGAAVGTGVAVAT
                     KGDQIVLGTGQKIKVVLDAPLKVTRTGS"
     gene            complement(3174..5114)
                     /locus_tag="VFR25_01190"
     CDS             complement(3174..5114)
                     /locus_tag="VFR25_01190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9939694.1"
                     /translation="MSRVGAPAPAPDGSFLIVPVTTYDIEENRGRARLWRVPRQGEAR
                     PLTSADASSTEPALSPDGTRLLYTRCQGDEKPQIHILSLQGGEPERLTEMPLGAMDPR
                     WFPDGKRIAFVAFLLADAPTSAATKELLEKRAKDPVKAHVTSDRIYRFWDKWLTGGER
                     PHLFVLDLATRALTDLTPDGAGWLDFMDSTGHYDIAPDGSEIAFSANSSQPPHYLLRW
                     AIYTVPTSGQGPVRCLTPEGSPENVSDALRPRYSPDGRSIVYGAQRDPFFYADKVRLV
                     RYDRAKNTHQTLTEAWDRSAGAWEFLADGSLAIDAEDRGKGALFHLPAQESTPRPLGI
                     AGTVGGLRVGRDGRLYFTIQSLSAPAEAASVKPDGSDLQVHTSFNAPILAEIAMGEVR
                     EMIFQGADNDPVQMYVTLPPNFDPSKRWPLVHVIHGGPHAISGDNFHFRWNPHLFAAR
                     GYVVATVNFHGSTSWGQEFAACIQGGHGDKPFTDIMRATDVLIETGFVDPARMAATGG
                     SYGGYLVCWIAGHTDRFRCLVNHAGVFDLLAEYGSDVTQGRHQAYGGEPWDRLEAIDR
                     WNPARFARGFTTPMLILHGERDYRVPHTQALAAYNIYQAKRVDSRLVFFPDENHWVLK
                     PKNSLVWNREVREWLDRYLGQA"
     gene            complement(5340..6482)
                     /locus_tag="VFR25_01195"
     CDS             complement(5340..6482)
                     /locus_tag="VFR25_01195"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939695.1"
                     /translation="MASRAQKIEVQTFRSEDLKPLLQSAGAPCVSIYLPTSRRFPEKR
                     QDPLRYRKLVDKAAALLKPNDAEAMIAPLRALEQESHWERSLDGFAAFLSPSFAAAYR
                     VSAPVPEIAVVADSFHVKPLLRLKHEATRYLVLAVSQKSARLYEGGPLGASAVDVPGL
                     PQSVNDALGAVVNERRSKTVHAGGIAHHGYGVGKEETKEDLLSYFRVIDKALREFLHG
                     ERAPLLLAAVKYYQPIYREANTYPHLIDAMLEGSYEHANGDKIHADAWPIVSAAASHV
                     FEPWVERYKTSAPSGLATDRIESVAEAVIQGRVSCVLAAEGETVWGILDRATGSVERR
                     ERQLDAADDDLLDDLCEEALKRGGEAFVVPRASMPTASPIAAVFRF"
     gene            complement(6486..6719)
                     /locus_tag="VFR25_01200"
     CDS             complement(6486..6719)
                     /locus_tag="VFR25_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET9939696.1"
                     /translation="MPAKIIIRNNGPIRIEGEFTIVDQDGREFDLAGRTAITLCRCGH
                     STKKPFCDSTHKTCGFDSTVVAIALPPPAPKPV"
     gene            6853..8532
                     /gene="ettA"
                     /locus_tag="VFR25_01205"
     CDS             6853..8532
                     /gene="ettA"
                     /locus_tag="VFR25_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET9939697.1"
                     /translation="MAPQYIFTMVNLGKVHPPNKEVLKGIYLSFYPGAKIGVLGQNGA
                     GKSTLLRIMAGVEEPTSGEARPAKGIKVGFLPQEPVLDPSKDVLGNIEEGVAETKALL
                     NRFNELNMKLGESLSDAEMEKVMEEHGRVMAEIEAVNAWELDRTLEIAMDALRVPPGD
                     AKVETLSGGERRRVALCRLLLTRPDLLLLDEPTNHLDAESVAWLERFLHDYPGTVVAV
                     THDRYFLDNVAGWILELDRGQGIPWEGNYSSWLDQKKNRLALEEKAESSRQRTLAREL
                     EWIRMSPRARQAKSKARVTAYEELLQQDQEKRSEVTEITIPAGPRLGNLVVEATDLAK
                     GYGDRALIEDLSFRLPPGGIVGIIGPNGAGKTTLFRMIAGQEKPDGGTLRLGDTVKLS
                     YVDQSRDALDGKKTVWAEISGGEDLIELGTRSVPSRSYVAAFNFKGADQQKPVKNLSG
                     GERNRVHLAKVLKSGGNLLLLDEPTNDLDVDTLRALEEAILSFAGCVCVITHDRWFLD
                     RIATHMLAFEEDGSVVWFEGNFQDYEANRHMRLGAAADQPHRLKYKKLVRE"
     gene            8846..>10819
                     /locus_tag="VFR25_01210"
     CDS             8846..>10819
                     /locus_tag="VFR25_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9939698.1"
                     /translation="MTRLLAPASLAVLLVSVPLALPAAARAADLDTRYGAPFSTLPMY
                     QTNTSVLMRDWNGDGLLDVTVTCNADDRVSTLKGVGQGAFGGRIDFSGPDNPYKVAAG
                     DINGDGTADMVLALYDANSVRVYYLVPGDVSYSTFADLPTGSGPVDVAVADIDQDGDN
                     DIVTSNFNSNNISVLRNSGGGTFLPHADYATGQNPWGMAVGRVNADAYPDVVTADFSS
                     STVTLWLGSSTGTLTFGYSLSAGSNPTDVALVDLDADGVQDMALANLNASSITLVDAY
                     GNIALQVVGTIPSLSGYEVVAADFNNDGKVDLAIAGSENASGSGTVHLGTGVGAFGPR
                     HDFATGGNLSIGMSAGDLDGDGKADLALASSPNNVSLLRGNGDGTFGVNRAVAPDGVN
                     ADFKRVALAEMTGDSHLDLILAGNANVRVARGNGSGAFSFVGQSGAPAVDIAVADLDG
                     DGDRDVASVRGTAFTNLAISYNPGNGAIATGAIYTVGGIAIAVAAGDVNHDGRPDLVV
                     GCDVPGDLFFLRNLGDGSFAPATSVAGSGWRRAVAVVDVNGDGDPDIVAANYLADNVE
                     VYPGDGSGGFGPSTTFATGHRPVSIGTGDFNADGAIDLALACEGNTISVLLNSGSGTF
                     LPATAYVKPGTWSGIAVGSADQDASPDLFVVDGY"
BASE COUNT         1771 a         3681 c         3620 g         1747 t
ORIGIN      
        1 tctcgcggag ctcacgtccc tcttccagct cggaggaacg ctccagatga acctcccgct
       61 cgagaccgtc gccgagatca ccgtgcgacg cgtcgcgagc tcgctccact cgtacgatgc
      121 gacgctcttc ctgatcgacg cggccggacg gcgcctcgtg ccgcgcgcga ccttcggcct
      181 caccgcgcgc ggacccgagc ccgaggccgc cgtgggcgag ggggcgctgg gctgggccgc
      241 ccgggcgcgc gagcccatcc tgatgcgcgc cgccgaccac gaagcgcgct tcgccgactt
      301 cttcaccaag cacccggacg cgggctcggt cctgatcctg ccggtttgcg tggagaagcg
      361 gtgcatcgcc gtgctccagg tctgtcgcgc ctcctgcgcc gatcccttcc ggatcgagca
      421 ccgtgacgtg gggcagctct tcgccgacaa cgtcgcgccg gtgatcgacc gcgcgcaggc
      481 gcagctccgg ttccgcgaag cggcggccgt ggccggcacg gtgacccctc cggcggagga
      541 gatcgcttcg ggcgccttcc gcgatgtctt cctgacctcg gccgcgaacg agctcaagtc
      601 gccgctcacg agtctcgtcg cgtacaccga agtcttcgac cagaacgatc cggcgatgac
      661 gccggccacg cggcgcgagt tcacggcgcg gctctacgag gaagcacagc gcatgctcgg
      721 cctcgtcgac gacgtgctcg accttgtccg tctcgagctg gggcgctatc tcctcgacgt
      781 gcgcctcgga agcgtgaatc aggccgtccg cgcggcgcgc gatctcgtgg cccccttcgc
      841 ggagagccgc tcggtggcga tcgatctcga tctcgaggga tccgtccccg accagcactt
      901 cgaccccgcc aagatccgcc agtcgctggt ccacatcctg cgccacgcga tccgcttctc
      961 gccgcagcgc tccagggtcc ggctccgctc catcgtggac ggcgaggggg tgctgatcga
     1021 ggtgaaggat tcgggtccga ccattcccgc ggaagcgtcg gaggcgatgt tcaatctaga
     1081 gtccgtgggc gatcagttcg gcaagcggtg caaggagcgc cagggcttcg gactccatct
     1141 cgcgcgccgc ttcgtcgagc tccacggcgg ccgcgtgggc gcgggcccgg cgcccgatgg
     1201 cggtgccgtg atctgggtgc gcctgccgcg cggtgaagat ctctcgagcc tcctcggctc
     1261 ggctccattc atggaggact tcgcgaagtc ctagcttcgc gtcaggacac ggcgcgccgc
     1321 gagcgtgccg ccgatccgag cgccgcctgc accgtcgcca gctcccccac gttctcgagc
     1381 gtgagcatcc ccaccagcgc accgtcttcc tcgaggaccg gaagcgccgg gcagcccctc
     1441 tcctgcatgc gcaggacggc cccctccagc atctcgcgcg gatgcgccga tccgcacgtg
     1501 cgccgggcga tctcgtcgac gcgcacgttc gtcttcccct cgctcagcgc ccgaaagaga
     1561 tccgcgcgaa gcaggatgcc gacgggcggg tcccccggca aaccgatgac cggaaagtcc
     1621 tgctggtgtc cattcaggag gagctcgacc gcgcgcgaca cgggctcgtc tccctgaagc
     1681 gcgcgaaagt cccggatcat cgccttccag accggaacgc cgtcgaacgc cgatcgcatc
     1741 tgcaccgccg ccgattcctg ccccgccccc aggaagacga acatcgcgat gaagaggagc
     1801 atcgggttcc agatgaggcc gaggaggccg aagaggatcg cgatcccctg tccgatcgag
     1861 gccgcgatcc gcgtggcctt cacgtagtcg agccggatcg cgaggagcgc gcggagcacg
     1921 cgcccgccgt ccatcggaaa ggccgggatc atgttgaaga tcacgaggag gacgttcatg
     1981 ctgaggagct tcgtcacgaa gttgtgctcg agggcgtctg ctgtcgcgaa ggtggcgaga
     2041 tttcccgtgg ccgcgatgat cgcgaggagg atggaggcga tcaccacgtt caccgcgggc
     2101 cccgccaggg ccaccgcgat ctcctggacc ggcttgtccg ggatccgctc cagacgggcg
     2161 acgccgccga tgggaaggag cgtgatgtcg cgcgtcctca ccccgaagcg ccgcgccgtg
     2221 agcgcgtgcc ccagctcgtg gagcacgagg caggtgaaga gggcgatgat gagggcgatc
     2281 gcctcgacgc cccgcttggg atcgccctgc gcttcggcga accaggcata ggggaagata
     2341 aggaagaagg tcgcgtgcac gcggagcggg atgcccgcga gcgtcgcgac cttccaggac
     2401 cagcgcatct agtgcgtcag gaaccggtgc gggtcacctt gagcggagcg tcgagcacga
     2461 ccttgatctt ctgccccgtt ccgagaacga tctgatcccc tttcgtcgcg accgcgacgc
     2521 cggtgcccac ggccgcgccc accgcggcgc ccttggccac gtcaccggac ccatgcccca
     2581 gcacgccgcc gatgatgccg cccgcgacag cgcctccgcc gatctcgagc gcgctctcct
     2641 tggcgtcccc cttgccctcc aggcggagcg gcacggcgga gatgtcatag gtcgtcccgt
     2701 ccggcagctt gatgctgtcg taacggagcg tcagcttggc cccgcccgcg acgcgtcctg
     2761 ctccgtccac gtaggagagg tggccgcgga tggtcgagcc ggccggcacg gcggtggcgc
     2821 cgtccacggt cttggcgagg gtggtgcgga tgctgaaggg gtcgccgaca tgattcttgc
     2881 cggagctgag cgtttgctgg agcgtgcccg agaaggacgt gccggtcgcc agggtatatg
     2941 tgtgcgacgt gggccccgac ttctgcggcg tctgctccgc cgtctgctcc tcaccggccg
     3001 gcgcgagctt gtcctcggtg gtcgtcgctc cctgttcctt cggctgctcc ttgtccgcga
     3061 ccgccttctg ctgatcgccg cagccggcga gcacgaaagc caggaccagg gcggcaccca
     3121 ttccgctcag aattcgcatc atgcgtttgt cgagcatcgt caacctcctt cgttcaggcc
     3181 tggccgagat agcgatccag ccattcccga acctcacggt tccagacgag cgagttcttg
     3241 ggcttcagga cccagtggtt ctcgtccggg aaaaagacca ggcgggagtc gacccgcttg
     3301 gcttggtaaa tattgtacgc ggcgagcgcc tgcgtgtgcg gcacgcggta gtcgcgctct
     3361 ccgtgaagaa tcagcatcgg cgtcgtgaat ccgcgcgcga accgcgcggg attccaccgg
     3421 tcgatcgcct cgagccggtc ccacggctcc ccgccgtagg cttgatgcct cccctgcgtg
     3481 acgtcgcttc cgtactccgc aagaagatcg aacacgcccg catggttcac gagacagcgg
     3541 aagcggtccg tgtgccccgc gatccagcag acgaggtacc cgccgtacga gccgcccgtc
     3601 gcggccatgc gcgcgggatc cacgaagcct gtttcgatca ggacgtcggt cgcgcgcatg
     3661 atgtcggtga acggcttgtc tccatgcccg ccctggatgc aggccgcgaa ctcctgcccc
     3721 cacgaggtcg atccgtggaa gttcacggtg gcgaccacgt agccgcgggc cgcgaagagg
     3781 tgcggattcc accggaagtg gaagttgtcc cccgagatcg catgcggccc gccgtggatg
     3841 acgtggacga gcggccacct cttcgacgga tcgaagttcg gaggcagcgt cacgtacatc
     3901 tggaccggat cgttgtccgc tccctggaag atcatctcgc gcacctcgcc catcgcgatc
     3961 tcggcgagga tgggagcgtt gaagctcgtg tgcacctgga ggtcgctccc gtccggcttg
     4021 acgctcgccg cctcggcggg cgcgctcagc gactggatcg tgaaatagag ccggccgtcc
     4081 cggcctaccc tgagcccgcc cacggtcccc gcgatcccga gggggcgcgg cgtcgactcc
     4141 tgggccggaa ggtggaagag agcacccttg cctcgatcct ccgcgtcgat cgcgagcgag
     4201 ccgtccgcga ggaactccca cgctcccgcg gaacggtccc acgcctcggt gagcgtctga
     4261 tgcgtgttct tcgcgcggtc gtagcgcacg agccgcacct tgtcggcgta gaagaaggga
     4321 tcgcgctgcg cgccgtacac gatcgaccgc ccgtcgggtg agtagcgcgg acggagcgca
     4381 tcggacacgt tctcgggcga gccctccggc gtcaggcagc gcaccggccc ctggccggac
     4441 gtcggaaccg tgtagatggc ccagcgcaga aggtaatgag gcggctggct cgagttggcg
     4501 gagaacgcga tctccgatcc gtccggagca atgtcgtaat gccccgtcga atccatgaag
     4561 tcgagccagc ccgctccgtc gggggtgaga tcggtgagcg cgcgcgtcgc gagatcgagc
     4621 acgaacagat gcgggcgctc accccccgtg agccacttgt cccagaagcg gtagatccga
     4681 tccgaggtca cgtgcgcctt caccggatcc ttggcgcgct tctcgaggag ctccttcgtc
     4741 gcggcggacg tgggcgcgtc cgcgagcagg aacgcgacga acgcgatccg cttcccatcc
     4801 gggaaccagc gcggatccat ggctccgagc ggcatctcgg tgaggcgctc gggctcgccc
     4861 ccctggagtg agaggatgtg gatctgcggc ttctcgtctc cctggcagcg cgtgtagagg
     4921 agccgcgtgc cgtcggggga gagcgcgggc tcggtggacg aggcgtccgc gctggtcaac
     4981 gggcgcgcct caccctgacg aggcacccgc cagagacggg cgcggccgcg attctcttcg
     5041 atgtcgtacg tcgtgacggg aacgatcagg aaggagccgt ccggcgcggg cgcgggggcg
     5101 ccgacgcggc tcatgcgcca gagatcctcg gccgtcatgg ggcgctggcc gcggttccgc
     5161 tcctggtgaa ggacggatga cggtcgtgtt tcggatcggg gctcggcgac gtgcacggca
     5221 cacctccggt ggggagaggc ccgcagccac cccggcgtgg gcgcgggact gacggaatgg
     5281 atcatcatag acgacccacg ccgccgacgg aaccgcggaa cgcggctccg ggaatcgggc
     5341 tagaagcgaa agacggccgc gatcgggctc gcggtgggca tcgaggcgcg tggaaccacg
     5401 aaggcctcgc cgccccgctt gagcgcttcc tcgcaaaggt cgtcgaggag atcgtcgtcc
     5461 gcggcgtcga gctgacgctc gcgccgctcc accgagcccg tggcccggtc gaggatgccc
     5521 cagaccgtct cgccctcggc cgcgagcacg cacgacaccc gtccctggat caccgcttcc
     5581 gccaccgact cgatgcgatc ggtcgcgagg ccgctcgggg ccgacgtctt gtagcgctcc
     5641 acccaaggct cgaagacatg tgaggcggcc gcgctcacga tgggccacgc atccgcatgg
     5701 atcttgtcgc cgttcgcgtg ctcgtagctc ccctcgagca tggcgtcgat cagatggggg
     5761 tacgtgttcg cctcgcgata gatcggctgg tagtacttca ccgcggcgag aaggagcggg
     5821 gcgcgctctc cgtgcaggaa ttcgcggagc gccttgtcga tgacgcggaa gtaggagagg
     5881 agatcctcct tcgtctcttc cttccccacc ccgtagccgt gatgcgcgat gccccccgcg
     5941 tgcaccgtct tcgagcggcg ctcgttcacc accgcgccga gcgcgtcgtt gacgctctgc
     6001 gggagcccag gcacgtccac ggccgacgcg ccgagcggcc caccctcgta gagtcgcgcc
     6061 gatttctggc tcacggcgag gacgaggtag cgggtcgcct cgtgcttgag acggaggagc
     6121 ggcttcacgt ggaacgagtc ggccaccacc gcgatctcgg gcacgggagc ggacacgcgg
     6181 tacgccgccg cgaacgaagg cgagaggaac gccgcaaatc cgtcgagcga gcgctcccag
     6241 tgcgattcct gctcgagcgc gcggagcgga gcgatcatcg cttcggcatc gttcggcttg
     6301 aggagcgccg ccgccttgtc gacgagcttc cggtagcgaa gcggatcctg ccgcttctcc
     6361 ggaaaacgac gcgaggtcgg taggtagatc gaaacgcaag gagcaccggc cgactggaga
     6421 agcggcttca agtcctcgct ccggaacgtc tggacctcga tcttctgcgc gcgggaggcc
     6481 atggctcaga ccggcttggg ggccggaggc ggaagcgcga tcgcgacaac cgtggagtcg
     6541 aagccacagg tcttgtgggt cgagtcgcag aacggcttct tcgtcgagtg accgcagcgg
     6601 cagagggtga tcgcggtgcg gccggccagg tcgaactcgc ggccgtcctg atccacgatc
     6661 gtgaactccc cctcgatgcg gatggggccg ttgttacgga tgatgatctt ggcgggcatc
     6721 gcgctcctcc caggtccgtc ggatcgggcc cacgccgcgc ccgcggcggt ccccctcgtc
     6781 gtctgctcca agtatatact cccggggaac cgtctcgcgt catcccccaa acccagagag
     6841 gtcagccgcc ccatggcgcc ccagtacatt ttcacgatgg tcaacctcgg taaggtccat
     6901 ccgcccaaca aggaggtcct gaaggggatc tatctctcct tctatccggg cgccaagatt
     6961 ggcgtcctcg gccagaacgg cgcgggcaag agcacgctcc tccgcatcat ggccggagtg
     7021 gaggagccga cgtcgggcga ggccaggccg gccaagggga tcaaggtcgg cttcctgccc
     7081 caggagcccg tgctcgaccc ctcgaaagac gtgctcggca acatcgagga gggagtggcc
     7141 gagaccaagg cgcttctgaa tcggttcaac gagctgaaca tgaagctggg cgagtcgctc
     7201 tccgacgccg agatggaaaa ggtgatggaa gagcacgggc gcgtgatggc cgagatcgag
     7261 gcggtcaacg cgtgggagct cgaccgcacg ctcgagatcg cgatggacgc gctccgcgtg
     7321 cctcctggcg acgcgaaggt ggagacgctg tcgggaggcg agcggcgccg cgtcgcgctc
     7381 tgccggcttc ttttgacgcg ccctgatttg ttgttgctgg acgagcccac gaaccacctc
     7441 gacgccgagt cggtggcttg gctcgagcgc ttcctccacg actaccccgg cacggtcgtc
     7501 gccgtgacgc acgatcgcta cttcctcgac aacgtcgccg gctggattct cgagctggat
     7561 cgcggacagg gcatcccctg ggaggggaac tactcgtcct ggctcgatca gaagaagaac
     7621 cggctcgcgc tcgaggagaa ggccgagtcc tcacgccagc gcacactcgc ccgcgagctc
     7681 gaatggatcc gcatgtcgcc gcgcgcccgg caggccaaga gcaaggcgcg cgtcaccgcg
     7741 tacgaggaat tgctgcagca ggaccaggag aagcggagcg aggtcaccga gatcacgatc
     7801 ccagccggcc cgcgcctcgg gaatctcgtg gtcgaagcca cggacctcgc gaagggttat
     7861 ggcgaccgag cgttgatcga ggacctctcg ttccgtctcc ctcccggagg gatcgtcggc
     7921 atcatcggtc ccaacggcgc cggcaagacg acgctcttcc gcatgatcgc gggccaggag
     7981 aagccggatg gcggcaccct ccgcctcggc gacacggtga agctctcgta cgtggaccag
     8041 tcccgcgatg ccctcgacgg caagaagacg gtgtgggcgg agatctcggg gggcgaagac
     8101 ttgatcgagc tcggcacgcg gagcgtcccg tcgcgctcct acgtggccgc cttcaacttc
     8161 aagggcgccg atcagcagaa gcccgtcaag aacctctccg gcggcgagcg aaaccgcgtg
     8221 cacctggcca aggtcttgaa gtcaggcggg aacttgctgc tcctcgacga gccgacgaac
     8281 gatctcgacg tcgacacgct gcgcgcgctc gaagaggcga tcctgagctt tgcgggctgc
     8341 gtctgcgtga tcacccacga tcgctggttc ctcgaccgca tcgcgacgca catgctggcg
     8401 ttcgaggagg acgggagcgt cgtctggttc gagggcaact tccaggacta cgaagccaac
     8461 cgccacatgc gcctcggcgc ggcggccgac cagccgcacc gcctcaagta caagaagctg
     8521 gtgcgggagt agcgccgaga gccctccccc ggcacacgcc gcacatgagc cgatttcgcc
     8581 ggtttctgcg ccttgtcagg cagaagcgtc gtcggtcgcg actggagagc acggtcgagc
     8641 ctgcggccgc cctacccatg ccctgacatc cgtttcgcca taatacacat tcacccaatg
     8701 gcttagcgga cccgggcgcc gcgtggggcg cccgtccgag cctctccccg ctatcccgcg
     8761 ctcctcgaca tcccgatcca gccttcaggt ctagcttcat gaggtcgctc ggttctgacc
     8821 gaccgtgctc taggggggca ctctcatgac acgtctgctc gcgcccgcat cgcttgccgt
     8881 tctgctcgtg tccgttccgc tcgccttgcc cgcagccgca cgcgcggccg acctcgacac
     8941 gagatacggg gctccgttct cgaccctacc catgtatcag acgaacacga gcgtcctcat
     9001 gcgggattgg aacggggacg ggctgctgga cgtcaccgtt acctgcaatg cggacgaccg
     9061 cgtctccaca ctgaagggcg ttggtcaagg cgccttcggt gggcgcattg atttctccgg
     9121 ccctgacaat ccgtacaaag tcgcggctgg agacatcaat ggcgatggga cggcggacat
     9181 ggtgctggcc ctgtacgacg cgaattccgt gcgggtctac tacctcgtgc ccggcgacgt
     9241 gagctacagc accttcgcgg atctgcccac cggctcgggg ccggtcgacg tcgccgtggc
     9301 tgacatcgat caagacggag acaacgacat cgtgacgtcg aacttcaact cgaacaacat
     9361 ctcggtcctg cgcaacagcg gcggcggtac cttcctccct cacgctgact acgcaaccgg
     9421 ccagaacccg tggggcatgg ccgtgggacg cgtgaacgcc gatgcctacc ccgacgtcgt
     9481 caccgcggac ttctcgagca gcaccgtgac gctctggctc gggtcgtcca cgggaacact
     9541 cacgttcggc tacagcctat cggccggcag caatcctacc gatgtggcgc tcgtcgacct
     9601 ggacgcggac ggcgtccagg acatggcact tgcgaatctc aatgcctcga gcatcacgct
     9661 cgtggacgcc tatggaaaca tcgccctcca ggtcgtggga accatcccga gcctctccgg
     9721 ctacgaagtc gtagcggccg atttcaacaa cgacggaaag gtcgacctgg cgatcgcggg
     9781 atccgagaac gcgagcggct cgggcaccgt gcacctcggg accggagtcg gcgcgttcgg
     9841 tccgcgccac gatttcgcca cgggaggaaa cctctcgatc gggatgtcgg cgggggatct
     9901 ggacggagac ggaaaggccg acctggccct cgcctcatcg ccgaacaatg tctcgctgct
     9961 ccgagggaat ggcgacggaa cgttcggcgt gaatcgagcg gtcgctcccg acggcgtgaa
    10021 cgcggatttc aagagagtgg cgctggccga gatgaccggg gacagccatc tcgatctcat
    10081 cctcgcggga aatgccaacg tgcgcgtcgc ccggggaaac ggctccggcg ccttctcctt
    10141 cgtaggacag agcggagctc cggcggtcga tatcgctgtg gccgatctcg atggcgatgg
    10201 cgatcgcgac gtcgcgagcg tcaggggcac cgcattcacg aacctcgcca tctcctacaa
    10261 cccggggaac ggggccatcg cgacgggcgc catctacacg gtgggaggaa tcgctattgc
    10321 ggtggccgct ggtgacgtca accacgacgg gcgccccgat ctggtcgtcg gctgtgacgt
    10381 tcccggcgat ctcttcttct tgcggaacct tggggacggc tcgttcgccc ctgcgacgtc
    10441 cgtcgcgggg agcgggtggc ggcgtgccgt ggccgtcgtc gacgtgaacg gagacggcga
    10501 ccccgacatc gtggccgcga attacctcgc ggacaacgtg gaggtctacc caggagatgg
    10561 ctcgggcgga ttcggaccga gcacgacctt tgcgacggga caccgaccgg tctcgatcgg
    10621 gaccggtgac ttcaatgcgg acggagctat cgatctcgct ctcgcatgcg aaggaaatac
    10681 gatttccgtc ctcctgaatt ccgggagcgg aacgtttctc cccgcgaccg cgtacgtcaa
    10741 acctggtacc tggagcggta tcgcagtcgg gagcgcggac caggacgcct cgcccgatct
    10801 cttcgtcgtc gacggttac
//
LOCUS       DASTKC010000033         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_970364, whole genome shotgun sequence.
ACCESSION   DASTKC010000033 DASTKC010000000
VERSION     DASTKC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970364"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2580
                     /locus_tag="VFR25_01215"
     CDS             <1..2580
                     /locus_tag="VFR25_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HET9939699.1"
                     /translation="LRTKDRLEDDPILIAGGLCTINPEPLAPFIDGFVIGDGEEVIQE
                     LCDLMVERRETGMPRAEVLRRMGAIEGVYVPALYDEVENGFGFVAREPRADAPASKPR
                     FRYVASLLPEYYPERPLISPTEITHDRLGVEVMRGCTRGCRFCLAGYMNRPLREKRAD
                     EVVKEVQCGIVHAGWDEVSLLSLSTTDYKQLPVVLHELDRFTNELGVSLSLPSTRVGT
                     LAPSVADKIGQGRKGSITFAPEAGTQRMRDVINKGIDEAELEYSVKLARDQGWGGIKF
                     YFMIGLPTETDADVDGIAETLRKSQEWAKGGNKRMHFNVGISPHVPKPHTPFQWEVQD
                     DMETLWRKIDRLKLGFRGMNNVRLKWRDPKTAFLEGVFARGDVRTADALEAAWKLGAR
                     FDGWSECFDFDLWMRAFEAIGMDPSIYLRPRDLEERLPWDHIRTPVVKKFLAAERQKA
                     YAAALTPDCRDHACYRCGAPCFTPKARDGRNLSLQLAPGQGAVPASLDFSVPPEVTTA
                     QIQERIQELTKKPIDTLRAPTAAAEAATNGKEHGANGHATHGANGKANGTNGANGTNG
                     ANGKTEGERAYGRRRRVWPTVGRGPQASRYRVAYEKLGDGRFTSHLDLVRIFDRAIRV
                     AKIPIAYTQGFNRHAKIAYGPPLSLGATSAAEYFDLDLAQACPWSTIEAMNEILPEGI
                     RILEGRPFTKASDSLMAAIGRADYWVRLTPYLMEELTRGEDVHRVRAELVQSIASFTA
                     SGDWPVAKQSHGQKGKTVNARLAVVDVALDPEAGPVGVRVASRLQATGYLRPDLLVRS
                     FLPSFDFDPRLLLVHREAMWVERGGVLRSPLEALEDSAFWRAVPVDSVDPAPADPAWE
                     VDA"
     gene            2573..4123
                     /locus_tag="VFR25_01220"
     CDS             2573..4123
                     /locus_tag="VFR25_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET9939700.1"
                     /translation="MRREIIINASASETRIALLEDKELVEILVERPEAVRRVGDIYKG
                     RVNAVLPGMQAAFVDLGLEKSAFLHASDLMPDSDLDDMFEEDEDGEDKKRGGGRGRRD
                     ETPPRIEEALKKGQEILVQITKEPIGTKGPRVTTQVSLAGRFLVFMPDHEHVGVSRKI
                     EDRTERTRLKQLLRELRPKGAGVIVRTVGAEQGRKEFQGDVKYLEQMWEKIEKQGERS
                     RAPALLHQEMEFTTGLIRDIFTEDVAQLVIDSKEEHKQILRYLSTYAPELKTRVKIYR
                     GEAPIFDHFGIEGEIEKAMERKVWLKKGGYITIDQTEALVAVDVNTGRFTGKRSQEET
                     ILKTNLEAAEEIARQLRLRDLGGIIVLDFIDMEEEANKKAVLDTLRNRLKRDRSRTKA
                     FAVSELGLIEMTRQRQRPSLANYFTENCAACEGTGRVLSLSSATLKIERMLRRVGQRS
                     KERQLLLRVHPDVAIHLVEQNADRLERLEKQYRFRVEIRDDPSLRRDEIRLFRGKTFE
                     EITKQYEH"
     gene            4174..4494
                     /gene="rplU"
                     /locus_tag="VFR25_01225"
     CDS             4174..4494
                     /gene="rplU"
                     /locus_tag="VFR25_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET9939701.1"
                     /translation="MSARYAVIEAQGLQFRVSPDEEHILPHLGAEEGRELTFDRVLLI
                     GGEGGITLGRPLVEGASVKAKVLGTERGPKITVGVYKRRKKYRRKIGYRDTLTRVRIL
                     DIVV"
     gene            4512..4793
                     /gene="rpmA"
                     /locus_tag="VFR25_01230"
     CDS             4512..4793
                     /gene="rpmA"
                     /locus_tag="VFR25_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET9939702.1"
                     /translation="MAHKKGVSSSKNGRDSASQRLGVKRFGGERVTAGSIIVRQRGTA
                     VAPGLNVGRGKDDTLFALKDGVVVFRRRGKSKMSVSVDPAPSASEAAHA"
     gene            4786..>5397
                     /locus_tag="VFR25_01235"
     CDS             4786..>5397
                     /locus_tag="VFR25_01235"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9939703.1"
                     /translation="MPKITVVGAGNVGASCAIYAAEAELGDVVLIDILEGVAKGKALD
                     LLEAGPVRGYDAYVEGSGDLRAMQGSDLVIVTAGLPRKPGMSRQDLLKANADIVRGVA
                     EAIKQHAPKAFVILVTNPLDVMAYLTYRITGFPRERVMGMAGVLDSSRFRTFLAEEIG
                     VSVEDVQAMVLGGHGDTMVPILDYATVSGIPVRNFIKPERLAEI"
BASE COUNT          965 a         1714 c         1933 g          785 t
ORIGIN      
        1 ctccgcacga aggaccggct ggaggacgac ccgatcctga tcgcgggcgg actctgcacg
       61 atcaacccgg agcccttggc gcccttcatc gacggcttcg tgatcggaga cggcgaggaa
      121 gtcatccagg agctgtgcga cctcatggtc gagcgccggg agacggggat gccgcgcgcc
      181 gaagtactcc gtcgcatggg agccatcgaa ggggtgtacg tgcccgcgct ctacgacgag
      241 gtcgagaacg gattcggctt cgtggcgcgc gagccgcggg cggatgcccc ggcgtccaag
      301 ccgcggttcc gctacgtcgc gtcgctcctg cccgagtact acccggagcg gccgctcatc
      361 tcgccgacgg agatcacgca cgaccgcctc ggggtcgagg tgatgcgcgg ctgcacgcgc
      421 ggctgccgct tctgcctcgc cggctacatg aaccgcccgc ttcgcgagaa gcgggccgac
      481 gaggtggtga aggaagtgca gtgcggcatc gtgcacgcgg gatgggacga ggtctcgctc
      541 ctctcgctct cgaccaccga ctacaagcag ctgccggtgg tgctccacga gctcgatcgc
      601 ttcaccaacg agctcggggt gtccctgtcg ctgccgtcga cccgcgtcgg gacgctcgcg
      661 ccgtccgtgg cggacaagat cggacagggc cggaagggga gcatcacgtt cgcgcccgaa
      721 gcgggaacgc agcggatgcg ggacgtgatc aacaagggaa tcgacgaggc ggagctcgag
      781 tactcggtga agctcgcccg cgaccaggga tggggcggca tcaagttcta cttcatgatc
      841 ggcctgccca ccgagaccga cgccgacgtg gacggcatcg ccgagacgct ccggaagtcg
      901 caggagtggg cgaagggcgg gaacaagcgc atgcacttca acgtgggcat ctcgccgcac
      961 gttccgaagc cgcacacgcc gttccagtgg gaagtgcagg acgacatgga aacgctctgg
     1021 cggaagatcg accggctcaa gctcggcttc cgcggcatga acaacgtgcg gctcaagtgg
     1081 cgcgatccga agaccgcgtt cctcgagggc gtgttcgcgc gcggcgacgt gcgcacggcc
     1141 gacgcgctcg aagcggcgtg gaagcttggc gcgcgcttcg acggctggtc ggagtgcttc
     1201 gacttcgatc tctggatgcg cgcgttcgag gcgatcggca tggatccgtc gatctatctc
     1261 cggccgcggg atctggagga gcggcttccg tgggaccaca tccggacgcc ggtggtgaag
     1321 aagttcctcg cggcggagcg gcagaaggcg tacgccgcgg cgctcacccc cgactgccgg
     1381 gaccacgcgt gctaccgctg cggcgcgccc tgcttcacgc cgaaggcgcg ggacggtcgg
     1441 aatctctcgc tccagctcgc accggggcag ggagcggtgc ccgcgtcgct cgatttcagc
     1501 gtgccgcccg aggtcacgac ggcccagatc caggagcgca tccaggagct cacgaagaag
     1561 ccgatcgata cgctccgtgc gccgacggct gccgccgagg ccgcgacgaa cggcaaggaa
     1621 cacggcgcca acgggcacgc cacccatggc gcgaacggca aggcgaacgg caccaacggc
     1681 gccaacggca cgaacggcgc caacggcaag accgaaggcg agcgcgccta cggtcgccgg
     1741 cgccgggtgt ggccgacggt cggccgcggc cctcaggcgt cgcgctaccg cgtggcctac
     1801 gagaagctgg gggacggccg gttcacctct catctcgatc tggtgcggat cttcgaccgc
     1861 gcgatccgcg tcgcgaagat cccgatcgcc tacacgcagg gcttcaaccg ccacgcgaag
     1921 atcgcgtacg gcccgccgct ctcgctcgga gcgacgagcg cggccgagta cttcgacctg
     1981 gatctggcgc aggcttgtcc gtggtccacg atcgaagcga tgaacgagat cctgcccgag
     2041 ggcatccgca tcctcgaagg gcggccgttc acgaaggcgt ccgactcact catggccgcc
     2101 atcggccggg ccgactactg ggtccggctc acgccgtacc tgatggagga gctcacgcgc
     2161 ggcgaggacg tgcaccgcgt gcgggcggag ctcgtgcagt cgatcgcatc gttcacggcg
     2221 tccggagact ggccggtcgc caagcagagt catggtcaga agggcaagac ggtcaacgcg
     2281 cgtctcgcgg ttgtggacgt cgcgctggac ccggaggccg gccccgtggg ggtccgcgtc
     2341 gcctcgcggc tccaggcgac cgggtatctc cgtccggatc tcctcgtgcg gagcttcctg
     2401 ccctcgttcg atttcgaccc ccgcctcctg ctcgtgcatc gcgaagcgat gtgggtggag
     2461 cgcggtggtg tccttcgttc tcccctcgag gcactcgagg attcggcatt ttggcgggcg
     2521 gtccccgtcg attcggtgga cccggccccg gcggaccccg cttgggaggt tgatgcgtag
     2581 agagatcatc atcaatgcga gcgcgagcga aacccggatc gcgctcctcg aagacaaaga
     2641 gctggtggag atcctggtcg agcggcccga ggcggttcgt cgggtcggcg acatctacaa
     2701 ggggcgtgtg aatgcggtgc tgccgggcat gcaggccgcg ttcgtggacc tcggtctcga
     2761 gaagagcgcg ttcctccacg cttcggatct gatgcccgac tcggacctcg acgacatgtt
     2821 cgaagaggac gaggacgggg aagataagaa gcgcggcggg ggacgtggcc ggcgcgacga
     2881 aacgccccct cgaatcgagg aagcgctcaa gaagggccag gagatcctgg tccagatcac
     2941 gaaggagccg atcggcacga agggcccccg ggtcacgacc caggtgtcgc tggcggggcg
     3001 gttcctcgtg ttcatgcccg accacgagca cgtgggcgtg tcgcggaaga tcgaggaccg
     3061 caccgagcga acgaggctga agcagctgct ccgcgagctc aggcccaaag gggcgggcgt
     3121 catcgtccgc accgtggggg cggagcaggg gcgaaaggag ttccagggcg acgtcaagta
     3181 tctcgagcag atgtgggaga agatcgagaa gcagggagag cgctcccgcg cgccggctct
     3241 cctccatcag gagatggagt tcaccaccgg cctgatccgg gacatcttca cggaggacgt
     3301 cgctcagctc gtcatcgatt cgaaggagga gcacaagcag atcctgagat atctctcgac
     3361 ctacgcgccc gagctcaaga ctcgcgtgaa gatctaccgc ggcgaggcgc cgatcttcga
     3421 tcacttcggg atcgagggcg agatcgagaa ggcgatggag cggaaggtct ggctcaagaa
     3481 gggcggctac atcaccatcg accagaccga agcgctcgtc gcggtcgacg tgaacaccgg
     3541 acggttcacg gggaagcgga gccaggaaga gacgatcctg aagacgaatc tcgaggcggc
     3601 tgaggagatc gcgcggcagc tacggctccg ggacctgggc ggcatcatcg tcctcgactt
     3661 catcgacatg gaggaggagg cgaacaagaa ggcggtgctc gacaccctcc ggaaccggct
     3721 caagcgggac cggtcgcgga cgaaggcgtt cgcggtgagc gagctcgggc tgatcgagat
     3781 gacgaggcag cggcagcggc ccagcctcgc caactatttc acggagaatt gcgccgcgtg
     3841 cgaggggacc ggccgggtgc tctcgctctc atccgcgacg ctcaagatcg agcggatgct
     3901 gcgccgcgtg ggacagcgct cgaaggagcg gcagctcctc ctccgggtgc atccggacgt
     3961 cgcgatccac ctggtggagc agaacgcgga ccgcctggag cggctcgaga agcagtaccg
     4021 gttccgtgtg gagatccggg acgatccgtc gcttcggcgg gatgagatcc gtctcttccg
     4081 cggcaagacg ttcgaggaga tcaccaagca gtacgaacac tgaacccggc ggacaacgcc
     4141 ggccacgaag cacgaacgaa aggatcgctg tctatgagtg ctcgctatgc cgtcatcgaa
     4201 gcccagggtc tccagttccg ggtctccccg gacgaggagc acatcctgcc ccacctcgga
     4261 gccgaggaag ggcgcgagct cacgttcgat cgggtgctcc tgatcggagg cgagggcgga
     4321 atcacgctgg gccgcccgct ggtcgagggg gcgtccgtga aggcgaaggt cctcggcacg
     4381 gagcgcggtc cgaagatcac cgtcggggtg tacaagcggc gcaagaagta tcggcgcaag
     4441 atcggatacc gggatacgct gacgcgggtc cggatcctgg acatcgtcgt ctagcggaag
     4501 gagcgaacga catggcgcac aaaaaaggtg tatcgagctc gaagaacgga cgcgactccg
     4561 cgtcgcagcg gctgggcgtg aagcgcttcg gcggcgagcg ggtcacggcc gggagcatca
     4621 tcgtccggca gcgcggcacg gcggtggcgc cgggcctcaa cgtgggccgg ggcaaggacg
     4681 acacgctgtt cgcgctcaag gacggcgtcg tggtgttccg ccgccgcggc aagagcaaga
     4741 tgtcggtgtc cgtcgacccc gcgccgagcg cgagcgaggc ggcccatgcc taagatcacc
     4801 gtcgtcggcg cgggcaacgt cggcgcgtcc tgcgcgatct acgcggccga ggccgagctg
     4861 ggggacgtcg tcctcatcga catcctcgag ggcgtggcca agggcaaggc gctcgacctc
     4921 ctcgaagcgg gaccggtgcg cggctacgac gcgtacgtcg aggggagcgg cgacctgcgt
     4981 gcgatgcagg gcagcgacct cgtcatcgtg acggcggggc ttccccggaa gcccggcatg
     5041 tcgcggcagg atctcctcaa ggcgaacgcc gacatcgtgc gcggcgtggc cgaggcgatc
     5101 aagcagcacg cgccgaaggc gttcgtgatc ctggtcacca atccgctcga cgtcatggcg
     5161 tacctcacgt atcggatcac cggcttcccg cgcgagcgcg tcatgggcat ggcgggggtg
     5221 ctcgactcgt cccgcttccg gacgttcctg gccgaggaga tcggtgtgtc ggtggaggac
     5281 gtgcaggcga tggtgctggg cggccacggc gacacgatgg ttccgattct cgactacgcc
     5341 acggtctcgg gcattccggt ccggaacttc atcaagccgg agcggctcgc cgagatc
//
LOCUS       DASTKC010000034         8021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1916859, whole genome shotgun sequence.
ACCESSION   DASTKC010000034 DASTKC010000000
VERSION     DASTKC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916859"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFR25_01240"
     CDS             complement(<1..452)
                     /locus_tag="VFR25_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HET9939704.1"
                     /translation="MMALIGWLGSVAGPAPACAAPPAGQETRALWVVRHAISTPGRVD
                     AVVDLAEQMNINTLLVQVRGRGDAFYKSDLVPPAETLEDAPAGFDPLERIIERAHARG
                     LEVQAWINVYLVWSAGALPHSPLHIVNAHPDWISIRADGRRLVEMVPE"
     gene            complement(548..1177)
                     /gene="rplQ"
                     /locus_tag="VFR25_01245"
     CDS             complement(548..1177)
                     /gene="rplQ"
                     /locus_tag="VFR25_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9939705.1"
                     /translation="MRHRKDHRKLSRTHSHRKALLRNLVTSLFIHERIETTVAKAKEA
                     RRLGERMITFAKRGDLPARRHVARFVHGDDVVRKLFDTVAPWYAERNGGYTRIVRIGR
                     RLGDAGETALLELVKSPELKEKLRHEAEEAAKAAKAAEKKGGAKTAKGKAGEAAAAKG
                     GEGEAAAEGGSPKEKRSAKPARGDKFGKKGVPTQHKPTTRARQRGKSGD"
     gene            complement(1246..2238)
                     /locus_tag="VFR25_01250"
     CDS             complement(1246..2238)
                     /locus_tag="VFR25_01250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9939706.1"
                     /translation="MKWKNLQMPKNVELEEKSSTNRYGKFSIEPLERGFGVTLGNALR
                     RVLLSSLPGAAVTAVKIDGVQHEFSTLTGVKEDVPEVLLNLKQMRFKIHSDGPKMATF
                     DARGTNAVTAADLKADPDIEILNPELHIATLNKDGEFRAEVEVGAGRGYVSAENQAVQ
                     DRPIGVLPVDSMFSPVTKVNFEVENTRIGQRIDYDKLTLEVWTDGSILPSDALAYGAK
                     ILKDHFALFVHFEEEIVEEVEEEVDEEFLRVKQLLERSVEELELSVRSSNCLKAANIK
                     TIGDLVTKSEGEMLKYRNFGRKSLKEIADILANMQLHFGMDVGKFKLGEKIEAA"
     gene            complement(2264..2893)
                     /gene="rpsD"
                     /locus_tag="VFR25_01255"
     CDS             complement(2264..2893)
                     /gene="rpsD"
                     /locus_tag="VFR25_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9939707.1"
                     /translation="MATFHEGKCRQCRREGEKLFLKGTRCFTEKCAFERRGYAPGEHG
                     KDRRPKETNYGTQLRMKQKARRIYGVLERQFRNYFEKAEHQKGVTGQNLLLMLERRLD
                     NLTYRLGFAPSRSSARRLVRHRHMMVNGKLVDIPSFQVRVGDEISVKPASKDLVAIQA
                     SVEHTKGRDMMSWLSLDPEKLSGRLLEYPTRGNIPTKVSEQLIVELYSK"
     gene            complement(2918..3319)
                     /gene="rpsK"
                     /locus_tag="VFR25_01260"
     CDS             complement(2918..3319)
                     /gene="rpsK"
                     /locus_tag="VFR25_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9939708.1"
                     /translation="MAEQAASAPKPKKKRERKVGINGIAHVQSTFNNTIVTIADMDGH
                     TVAWASAGKVGFKGSRKSTPFAAQIAAEAATKEALSLGLKKVEVWVKGPGAGREAAIR
                     SLQAAGLEISAIKDVTPIPHNGCRPPKRRRV"
     gene            complement(3370..3777)
                     /gene="rpsM"
                     /locus_tag="VFR25_01265"
     CDS             complement(3370..3777)
                     /gene="rpsM"
                     /locus_tag="VFR25_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9939709.1"
                     /translation="MARIAGVDLPNDKRVVIGLTYLFGIGNSTARKIIQMTGVSPDTR
                     IKNLTEEETARLRQVIENQFKVEGARRSEVAMSIKRLMDIGAYRGLRHRKNLPARGQR
                     TRTNARTRKGPKKTAGAMRKVVAAKKPAPKPAG"
     gene            complement(3793..3906)
                     /gene="rpmJ"
                     /locus_tag="VFR25_01270"
     CDS             complement(3793..3906)
                     /gene="rpmJ"
                     /locus_tag="VFR25_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9939710.1"
                     /translation="MKVKASVTKICDNCKVVRRKGVVRIICKNPRHKQRQG"
     gene            complement(3923..4141)
                     /gene="infA"
                     /locus_tag="VFR25_01275"
     CDS             complement(3923..4141)
                     /gene="infA"
                     /locus_tag="VFR25_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET9939711.1"
                     /translation="MAKEEGVQVEGTVVEPLPNAMFRVELENGHRVLAHISGKMRMNF
                     IKILPGDKVTVELSPYDLNRGRIVYRYK"
     gene            complement(4141..4926)
                     /gene="map"
                     /locus_tag="VFR25_01280"
     CDS             complement(4141..4926)
                     /gene="map"
                     /locus_tag="VFR25_01280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9939712.1"
                     /translation="MRAGGVKAVGILIHTPEEIAKIRESAQIVGRCLRMIASEIHPGV
                     STMHLDRLAERFIRDSGGEPAFKGYRGFPASICASINEEVVHGIPSEKRILREGDVIS
                     VDIGVRRNGWYGDAARTFAVGEIDPETAKLLAATERSLEEGMAKARNGGRLSDISFAI
                     EQEVKKDGFSVVRALVGHGIGRELHEEPQVPNYGRAGEGPRLQTGMTLAIEPMVNAGG
                     ADVMTLSDQWTVVTADSKRSAHFEHTVAVGPDGPEILSRIEGD"
     gene            complement(4923..5573)
                     /locus_tag="VFR25_01285"
     CDS             complement(4923..5573)
                     /locus_tag="VFR25_01285"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9939713.1"
                     /translation="MRVVLLGPPGSGKGTHGQRLAKQLGTPVISTGDILREAIAQQTR
                     LGQAAQADVKSGRLVADETVLGLIETRLGLTDAKNGFILDGFPRTVAQADGLKRILGG
                     QKIDRVVNLVVPADMLVQRLEARWTCPSCGAIYNMKTSPKPAKAGVCDRCGGELKQRE
                     DDRPEAVRTRLKVYEEQTAPLVAYYDREGALRNVDASGLPEEVYAAIERAIGQVAA"
     gene            complement(5570..6877)
                     /gene="secY"
                     /locus_tag="VFR25_01290"
     CDS             complement(5570..6877)
                     /gene="secY"
                     /locus_tag="VFR25_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9939714.1"
                     /translation="MLEKFQNIFRIPELKRRVLFTLGLLVVYRLGGHIPTPGVNSEAL
                     VAAFAQQTNSLFGLYDLFVGGSFAKATVFALGIMPYISVSIIFQLLGAVIPYFEKLQK
                     EGEEGRKKITQYTRVGTVALAAVQSLAFAKFLESMGSGGAPVVPHPGITFQLMTMITQ
                     TTGTILIMWLGEQITERGIGNGISLIIFIGIVARFPADIVNTIRAIRVGTLSPVIAAL
                     LAGMMVAVIAAVVAMTQAQRKIPVQYAKRVVGRKMYGGQATHIPLRVNTAGVIPIIFA
                     QSLIMLPSTLALYFQGSNMIQSVARLFAHGTWVYVIVYSLTIIFFTYFYTAIVLNPTD
                     MADNMKKYGGFVPGIRPGRKTSEYIDRILSRITLPGAIFLALIAVLPDLLIRSFNVPF
                     YFGGTSVLIVVGVALDTLQQIESHLIMRHYEGFVTHGRMRGRR"
     gene            complement(6887..7372)
                     /gene="rplO"
                     /locus_tag="VFR25_01295"
     CDS             complement(6887..7372)
                     /gene="rplO"
                     /locus_tag="VFR25_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET9939715.1"
                     /translation="MRIGQIKPAKGATKDRKRRGLGPASGLGGTAGKGHKGQLARTGG
                     KVARWFEGGQMPIQRRLPKVGFKNHTRVEFQVVNVGRLAAYGAGETVDRASLQSKRLI
                     DRVNLPVKLLGTGDVAGALKVSVDAVSASARQKIEGAGGTITLPEPRKYRPRGVKKTR
                     A"
     gene            complement(7474..7644)
                     /gene="rpmD"
                     /locus_tag="VFR25_01300"
                     /pseudo
     CDS             complement(7474..7644)
                     /gene="rpmD"
                     /locus_tag="VFR25_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
     gene            complement(7631..>8021)
                     /locus_tag="VFR25_01305"
     CDS             complement(7631..>8021)
                     /locus_tag="VFR25_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9939716.1"
                     /translation="LGKANEVADAIRKAGESAKKRMFEVPLRRTTIPHEVTGRFGAGR
                     VFLKPATAGTGVIAGGAIRAVLEAAGIQDVLTKSLGSGNPHNVVKATVAGLQGLRRAS
                     DVAALRGKTVDELLGIRRNKVESDASN"
BASE COUNT         1172 a         2806 c         2528 g         1515 t
ORIGIN      
        1 tcctcgggca ccatctccac gaggcggcgg ccatcggcgc gaatggagat ccagtcggga
       61 tgcgcattca cgatgtggag cggcgagtgg ggcaaggcgc cggcggacca gaccaggtac
      121 acgttgatcc aagcctgcac ttcgaggccg cgcgcgtgtg cgcgctcgat gatgcgctcc
      181 agaggatcga atccggcggg ggcgtcttcc agggtctcgg cgggaggcac gaggtcggac
      241 ttgtagaagg cgtcgccgcg gccgcgcacc tggaccagga gcgtattgat gttcatctgc
      301 tcggcgagat cgacgaccgc gtccacgcgg ccgggggtgc tgatggcgtg gcgcaccacc
      361 cagagcgcgc gggtttcctg gcctgcgggg ggcgcggcgc acgcgggcgc agggcccgcc
      421 acggaaccga gccacccaat caaggccatc atcaacacca ccgccactcg agccatgccc
      481 ggaccccaaa gagaaacgcc ggcgcaggat gccgcgccgg cgtggaaacg ttcaattcat
      541 cgcggctcta gtcgccgctc ttccctcgct gccgggcgcg cgtggtcggc ttgtgctggg
      601 taggaacacc cttcttgccg aacttgtccc cgcgggccgg cttggcgctc cgcttctcct
      661 tcggcgaacc gccttcagcc gcagcctcgc cctcaccgcc cttggccgcg gcagcctcgc
      721 cggccttgcc cttcgccgtc ttcgcgccgc ccttcttctc ggccgccttc gccgccttgg
      781 cagcctcctc ggcctcgtgg cggagctttt ccttcagctc gggcgacttc accagctcga
      841 ggagcgcggt ctcgcccgcg tcgccgagcc ggcgaccgat ccggacgatc cgcgtgtagc
      901 cgccgttccg ctcggcgtac cacggagcca cggtgtcgaa gagcttcctc acgacgtcgt
      961 ccccgtgcac gaaccgcgcg acgtgacgac gggcgggcag atcgccccgc ttcgcgaagg
     1021 tgatcatgcg ctcgcccagc cggcgagctt ccttcgcctt ggcaaccgtc gtctcgatcc
     1081 gctcgtggat gaagagcgac gtcaccaggt tccggagcag cgccttccga tgggagtgcg
     1141 tccggctgag cttccgatga tctttacggt gtcgcatggt ctaggaacct gtcctctcag
     1201 ggttcggcgg gaccgctacg aagcgctccc ggggcctttc gcccctcaag ccgcttcgat
     1261 cttctcgccg agcttgaact tgcccacgtc catgccgaag tggagctgca tgttcgcgag
     1321 gatgtcggcg atctccttga gcgacttgcg gccgaagttc cggtacttga gcatctcgcc
     1381 ctcggacttc gtgaccaggt cgccgatggt cttgatgttc gcggccttga ggcaattcga
     1441 ggaccgcacg ctgagctcga gctcttccac gctccgctcg agcagctgct tcacgcgcag
     1501 gaactcctcg tccacctcct cctcgacctc ctcgacgatc tcctcctcga agtggacgaa
     1561 cagagcgaag tggtccttca ggatcttggc gccgtacgcg agggcatccg acggaaggat
     1621 ggagccgtcc gtccagacct cgagcgtcag cttgtcgtag tcgatgcgct ggccgatccg
     1681 ggtgttctcc acctcgaagt tgaccttcgt caccggcgag aacatggaat caaccgggag
     1741 cacgccgatc gggcgatcct ggacggcctg gttctccgcc gagacgtagc cccggcccgc
     1801 gcccacttcg acttcggcgc ggaactcgcc gtccttgttc agggtggcga tgtggagctc
     1861 cggattcagg atctcgatgt ccggatccgc cttgagatcg gccgccgtca ccgcgttcgt
     1921 gccgcgcgcg tcgaacgtgg ccatcttcgg gccgtccgag tggatcttga agcgcatctg
     1981 cttgagattg aggagcacct cgggcacgtc ctccttcacc cccgtgaggg tggagaactc
     2041 gtgctgcacg ccgtcgatct tgacggccgt gaccgcggcg cccggcaagg acgagaggag
     2101 cacgcgccgg agcgcgttcc ccagcgtcac tccgaagccc cgctcgagag gctcgatgga
     2161 gaactttccg tatcggttcg tcgaggactt ctcttccagc tcgacgttct tgggcatctg
     2221 aagattcttc cacttcattc ttggattccc cctcctcgac cgtttacttc gagtacagct
     2281 ccacgatgag ctgctcggaa accttggtcg gaatgttccc gcgcgtcgga tattcgagca
     2341 gccgccccga gagcttctcg gggtcgaggc tgagccagct catcatgtcg cgtcccttgg
     2401 tgtgctccac ggacgcctgg atcgcgacga gatccttgct cgccggcttc acgctgatct
     2461 cgtcgcccac ccgcacctga aacgacggga tgtcgaccag cttgccgttc accatcatgt
     2521 gccggtggcg caccagccgt cgcgccgaag agcgcgaggg ggcgaagccg agccggtagg
     2581 tgaggttgtc gagccggcgc tccagcatga ggagcaggtt ctggcccgtg acccccttct
     2641 ggtgctccgc cttctcgaag tagttccgga actgccgctc cagaacgccg tagatgcgcc
     2701 gggccttctg cttcatgcgg agctgcgtgc cgtagttggt ctccttggga cgccgatcct
     2761 tgccgtgctc cccgggcgcg taaccgcggc gctcgaaagc gcacttctcc gtgaagcagc
     2821 gggtaccctt gaggaagagc ttctctccct cccggcggca ctgccggcat tttccttcgt
     2881 gaaaggtcgc catatcggtg cttcgtacct ccttcggcta gacgcggcgc cgcttgggcg
     2941 gccggcagcc attgtgcggg atcggggtga cgtctttgat ggccgagatc tcgagcccgg
     3001 ccgcctgcag cgaccggatc gccgcctcgc ggcccgcgcc cggccccttc acccacacct
     3061 ccaccttctt cagcccgagc gagagcgctt ccttggtcgc cgcctcggcc gcgatctgcg
     3121 cggcgaacgg cgtgctcttg cgcgagccct tgaatccgac cttgccggcg ctggcccagg
     3181 ccaccgtgtg gccgtccatg tcggcgatcg tcacgatcgt gttgttgaac gtcgactgga
     3241 cgtgggcgat gccgttgatg cccaccttcc gttcgcgctt cttcttgggt tttggtgccg
     3301 atgccgcctg ttccgccacg ttcgctcctt ctcttcaggg acggcgcgcc gtgcgcgccg
     3361 cgctcatcgt caaccggccg gcttcggcgc gggcttcttc gcggccacca cttttcgcat
     3421 ggcgccggcg gtcttcttgg gtcccttgcg ggtgcgcgcg ttggtgcgcg tgcgctgccc
     3481 gcgggcgggc aggttcttgc gatggcgcag gccacgatag gcgccgatgt ccatgagccg
     3541 cttgatggac atcgcgacct cgctccggcg cgcgccttcc accttgaact ggttctcgat
     3601 cacctggcgg agccgtgcgg tctcctcttc ggtgaggttc ttgatgcgcg tgtccggact
     3661 cacccccgtc atctggatga tcttccgggc cgtcgagttg ccgataccga agagatacgt
     3721 cagcccaatg acgacgcgct tgtcgttggg tagatcgacg ccggcgatac gagccaaacc
     3781 ttgcctcctg tcctatccct gccgctgctt gtggcgcggg ttcttgcaga tgatccggac
     3841 cacacccttg cgccggacga ccttgcagtt gtcgcagatc ttggtgaccg aagccttgac
     3901 cttcatgacc gctccgtttc ttctacttgt accggtagac gatgcgcccg cgattgagat
     3961 cgtacgggct caattccacc gtgaccttgt ctccgggcag gatcttgatg aagttcatcc
     4021 gcatcttgcc ggagatgtgc gccaggacgc ggtgtccgtt ttccaattcc actcggaaca
     4081 tcgcgttcgg gagaggctcg accaccgtcc cctcgacctg aacgccttct tctttcgcca
     4141 tcaatcgccc tcgatccggg acaaaatctc agggccgtcc ggaccgaccg cgacggtgtg
     4201 ctcgaaatgc gccgaccgct tgctgtccgc cgtcacgacc gtccactggt cggagagggt
     4261 catgacgtcc gcccctccgg cgttcaccat cggctcgatc gcgagcgtca tccccgtctg
     4321 gagacgggga ccttcacccg cacggccgta gtttggaacc tgcggctcct cgtggagctc
     4381 gcgcccgatg ccatgtccca cgagcgcccg caccacactg aagccgtcct tctttacttc
     4441 ctgctcgatc gcgaacgaga tgtccgagag ccggcccccg ttccgcgctt tcgccattcc
     4501 ttcttcgagc gaccgctcgg tcgccgcgag gagcttcgcg gtctcggggt cgatctcccc
     4561 caccgcgaag gtccgcgccg catcgccgta ccagccgttt cgccggacgc cgatgtcgac
     4621 cgagatcacg tccccctcgc ggaggatccg cttctcgctc gggatcccgt gcaccacctc
     4681 ctcgttgatc gaggcgcaga tgctcgccgg gaatccccgg tatcccttga acgccggctc
     4741 gccccccgag tcccggatga accgctcggc cagccggtcc agatgcatcg tcgagacgcc
     4801 ggggtgaatc tccgacgcga tcatccggag gcaccgcccg acgatctggg cgctctcccg
     4861 gatcttcgcg atctcctcag gcgtgtggat caggatccct acggccttga ctcctccggc
     4921 cctcacgccg cgacctgtcc gatcgcgcgc tcgatcgccg cgtagacctc ctcgggcaag
     4981 cccgacgcgt ccacgttacg aagcgcgccc tcgcggtcgt agtacgcgac cagcggggcc
     5041 gtctgctcct cgtacacctt gagccgcgtc cggaccgcct cggggcggtc gtcctcgcgc
     5101 tgcttcaatt cgccgccgca gcggtcgcac accccggcct tcgcaggctt cggggaagtc
     5161 ttcatattgt agatggcgcc acagctcggg caggtccagc gcgcctcgag ccgctgcacc
     5221 agcatgtccg ccggcaccac caggttcacc acccggtcga tcttctggcc gccgaggatc
     5281 cgcttcagcc cgtcggcctg cgcgaccgtg cgcgggaatc cgtccaggat gaagccgttt
     5341 ttcgcgtccg tgaggccgag ccgggtctcg atcagtccga gcaccgtctc gtccgcgacc
     5401 agccgccccg acttcacgtc ggcctgcgcc gcctggccga gccgcgtctg ctgcgcgatg
     5461 gcctcgcgga ggatgtcccc cgtcgagatc accggggtcc ccagctgctt cgcgagccgc
     5521 tggccgtgcg ttcccttgcc gctccccgga ggaccgagga gcaccaccct catcgacgcc
     5581 cccgcattct cccgtgcgtc acgaatcctt cgtagtgccg catgatcaga tgggattcga
     5641 tctgctgcag ggtgtcgagg gccacgccga ccacgatcag cacgctcgtg ccgccgaagt
     5701 agaacggcac gttgaacgag cggatcagga ggtccggcag gaccgcgatc agggccagga
     5761 agatggcgcc cggcagcgtg atgcgcgaca ggatgcggtc gatgtattcg gaagtcttcc
     5821 gccccggccg gatgcccggg acgaagccgc cgtatttctt catgttgtcc gccatgtccg
     5881 tcgggttcag gacgatggcg gtgtagaagt aggtgaagaa gatgatcgtg agcgagtaga
     5941 cgatgacata gacccaggtt ccgtgcgcga agagccgcgc gaccgactgg atcatgttgc
     6001 tcccttgaaa gtagagcgcc agcgtgctcg gcagcatgat caacgactgc gcgaagatga
     6061 tcgggatcac gcccgccgtg ttcacgcgga gcgggatgtg cgtcgcctgg ccgccgtaca
     6121 tcttccggcc cacgacgcgc ttcgcgtact ggaccgggat cttgcgctgg gcctgcgtca
     6181 tggccacgac cgccgcgatc accgcgacca tcatgccggc gaggagcgcc gcgatcaccg
     6241 gcgagagcgt gccgacgcgg atcgcccgga tcgtgttcac gatgtcggcc gggaagcggg
     6301 ccacgatgcc gatgaagatg atgagcgaga tgccgttccc gatccctcgc tcggtgatct
     6361 gctcgcccag ccacatgatg aggatcgtcc cggtggtctg ggtgatcatg gtcatgagct
     6421 ggaacgtgat cccgggatgc ggcaccaccg gcgccccacc cgagcccatc gactccagga
     6481 acttggcgaa tgcgagcgac tggaccgccg cgagcgccac cgtgccaacg cgggtgtact
     6541 gggtgatctt cttccgcccc tcttcccctt ccttctggag cttctcgaag tagggaatca
     6601 ccgcgccgag gagctggaag atgatcgaga ccgagatgta cggcatgatc ccgagcgcga
     6661 agaccgtcgc cttggcgaag cttccgccga cgaacaggtc gtagagaccg aagagcgagt
     6721 tcgtctgctg cgcgaacgca gccacgagcg cctcgctgtt cacccccggg gtcgggatgt
     6781 gcccgcccag gcggtagacc acgaggagcc ccagcgtgaa gaggacccgg cgcttcagct
     6841 ccgggatccg gaagatgttc tggaatttct cgagcattgc tctccgttac gcccgggtct
     6901 tcttgacgcc gcgcggacga tacttgcgag gctccggcag cgtgatggtg ccgcccgccc
     6961 cttcgatctt ctgacgagcc gacgcgctga ccgcgtcgac cgacactttg agtgctcccg
     7021 ccacgtcgcc cgtgccgagg agcttgaccg gaaggttgac ccgatcgatc agccgcttcg
     7081 actggaggga cgcgcgatcc acggtctccc cagcgccgta cgcggcgagc cggcccacgt
     7141 tcaccacctg gaactccacg cgggtgtggt tcttgaaccc gaccttgggg agccggcgct
     7201 ggatgggcat ctggccgccc tcgaaccagc gcgcgacctt gccgcccgtc cgggcgagct
     7261 gtcccttgtg gcccttgccc gcggtgccgc cgagacccga agccgggccc aagccgcggc
     7321 gcttccggtc cttcgtggct cccttcgcgg gcttgatctg tccaatcctc acgacgtcct
     7381 cccggccgcg gccttggcgc gcggcttggc cggcgtcttg gcggcgcgcg gcttggcggt
     7441 cttccctgcg ggcgacttcg cgccgtccgc gggctcgaca cgcacgagat agcgaacctg
     7501 gtccaccatg ccgcgaatcg cgggcgtgtc ttcgtggacc accgaatgac gaatgcgccg
     7561 gaggccgagc gcggcgagcg tccgcttgtg cttcttcagc gtgcggatct tgctcttctc
     7621 ctgcgtgatc ttaatttgag gcatcgctct ccaccttgtt gcgtcgaatg ccgagaagct
     7681 cgtccacggt cttcccgcgg agcgccgcga cgtcgctcgc gcggcggagg ccctggagtc
     7741 cggccaccgt ggccttcacc acgttgtgcg gattgcccga gccgagcgac ttggtgagca
     7801 catcctggat ccctgccgcc tcgaggacgg cgcggatggc gccgcccgcg atcacgccgg
     7861 taccggccgt ggcgggcttc aggaagacgc ggccggcgcc gaagcggccc gtgacctcgt
     7921 gagggatcgt ggtccgccgg agcggaacct cgaacatgcg cttcttcgca gactcgcccg
     7981 ccttgcggat cgcgtcggcc acctcgttcg ccttaccgag g
//
LOCUS       DASTKC010000035        15804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_970754, whole genome shotgun sequence.
ACCESSION   DASTKC010000035 DASTKC010000000
VERSION     DASTKC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15804
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970754"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFR25_01310"
     CDS             complement(<1..1023)
                     /locus_tag="VFR25_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET9939717.1"
                     /translation="MSVRRALAGARAAGLLLILAGAGISRAQAAPVRYTITGNKVSVY
                     NLVGSLEIVPGTGSAVTAEVEVAGPDAAKLKVLTGAVRGRQTLRVLYPDDHVIDRSMG
                     RNSRTTLKVRDDGTFGDSKDGVDKGDRMTISGSGEGLEASADVKLLVPAGTNLMVYWG
                     HGSASVSRVDADVFLDGASMPVHAEDLHGPFHADVGSGEIEILGSDAAVNIDTGSGAV
                     RLRDVRTTEGILVDTGSGEVSGENLKAATVAIDTGSGSINLENVDAKSVNMDTGSGEI
                     DLDLAGDVSTLVVESGSGDITVSFPPGVGAQVSVESGSGGIRSELALETQVRKHDALV
                     GRIGDGR"
     gene            1243..1773
                     /locus_tag="VFR25_01315"
     CDS             1243..1773
                     /locus_tag="VFR25_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9939718.1"
                     /translation="MPQAIDRTDVTRAQDGDTRAFERLYRSHVARVHGLARRMIGPEH
                     AAEVTQDVFVRVWEKLGTFRGESAFGTWLHRVAVNVVLHRRAALKLERMRFDDDEEAL
                     DVIPAPVRHATAEFGMDFDRAIDKLPDGARVVFVLHDVEGFKHEEIAGFLGITSGTTK
                     AQLHRARMILRQHLTR"
     gene            1787..2485
                     /locus_tag="VFR25_01320"
     CDS             1787..2485
                     /locus_tag="VFR25_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9939719.1"
                     /translation="MSDRYTDLISDYLDGTLDPGTHRQVERHLAGCTECAALLGDFEH
                     VVGRARVLAEEDTPPAEDLWPGIEARLRRAAHTPLPIPVTITAVPERRSLKPRISFTI
                     PQLAAACLAVVALSAAGALYIGSATRKAETTASVTQQLPAPAAATMTESQDAIKDVAR
                     LKDILASRRNELDPATVAALEGSLRAIDNAIRQGQLALAADPKNPYLRDHLDDTMRRK
                     VDLMRRATMLASAD"
     gene            2509..3483
                     /locus_tag="VFR25_01325"
     CDS             2509..3483
                     /locus_tag="VFR25_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET9939720.1"
                     /translation="MSLPVVLAAVAVLSLQADVDTTFAVKKGAALQVENFAGEVVLSA
                     WDKNAVRIQAPEADDARVIVKPTPRGLVIKTGSKHVAAGSVDLRITVPAWMDLDVSGV
                     QTDVSIDGARGRVRVATIHGDIAVSGGRGQIELNAVDQDIHLADASGVVVSETVNGDL
                     TLQRIESDSVDVSTVNGEIFYEGTLRSRGVYRFTSHNGDIAVAIPTTTSATVNVSTFS
                     GEFASDFPVTLQESRPGRRYCFTLGKGGARVDLESFQGTIHVFRPGTRGPVTDVENQR
                     MERLQERMEKVKEKFERRKNEYKYQTKSSKDDAEDDSDDDDDDSEDSK"
     gene            complement(3676..4446)
                     /locus_tag="VFR25_01330"
     CDS             complement(3676..4446)
                     /locus_tag="VFR25_01330"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9939721.1"
                     /translation="MSLLRGRIAIVTGGARGIGAACCEKLAREGASVLVTDVLDDEGK
                     ALADSIRSSGGRALYRHHDVSSEPEWTAAVETAVREFGGLHVLVNNAGIARIEDVEEE
                     TLEGYEKLIAVNQTGAWLGMKAAVPAMKRAGLGSIVNVASIYGTVGGNGNAIAYHASK
                     GALRLMTKSAALRYAREGIRVNSVHPGFIDTPMVGPFLAKSEGAPNPMLDYILTHTPM
                     GRVGRPEEVANAVAFLASDESSYVTGSELYVDGGWTAA"
     gene            complement(4443..6161)
                     /locus_tag="VFR25_01335"
     CDS             complement(4443..6161)
                     /locus_tag="VFR25_01335"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET9939722.1"
                     /translation="MIPFEERTDPRTGEIYWAVNREGEALLDDPLLNKGTCFSPEERE
                     AFHLHGLLPPGVATEEEQRARAYENYLRSGSEVQRYLFLAALQDRNETLFYRLLLDHL
                     EEMAPIVYTPTVGKVCEQFSHIYRRPRGVYVSIEDRGRVVEILRHASQPDVRVIVATD
                     NEAILGIGDQGVGGMGIPIGKLALYTAGAGLHPSRCLPLDIDVGTDNQALLDDPLYLG
                     VPRRRVRGPEYDSLIDEIVDAIRDVFPRALLQWEDLSNRNAFRVLTRHRNQIASFNDD
                     IEGTGAVVTAGIRSATHQVGRAVAEERIVFFGAGASGAGSALAVRRALAEAGVPSREL
                     SSRVLCLDSKGLIVADRPGLEAEKAAIAAPVELVRSWGHVGDGPVTLEQVVASFHPTV
                     LVGASGQPGAFTEPVVRAMFQHCARPVILPISNPTSKCEATPAELLGWTGGAAVVGTG
                     SPFPPVEIGGTVHEIGQGNNALVFPGVGLGAVAVEARRLTDEAFAAASRAVFECTDKS
                     GRPGAPIYPPLSRLREISFRVALAVATTLVSEGAAPELEPAAVEARIRAMIWEPVYRP
                     YRRIRT"
     gene            complement(6232..6699)
                     /locus_tag="VFR25_01340"
     CDS             complement(6232..6699)
                     /locus_tag="VFR25_01340"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9939723.1"
                     /translation="MSLRQLQRLFEIDAYAFGVNVKSLSHEDSLVQPPAGGNCLNWVA
                     AHIVASRGAILDLLGEAQVWDADRNDRFKRGSRPVQDTKDSLPFDLIVADFRRSQERI
                     QAGLARLSDEELLEKRGDDTLADKLHFLQFHEAYHIGQAGLLRRMAGKECAIP"
     gene            complement(6713..7303)
                     /locus_tag="VFR25_01345"
     CDS             complement(6713..7303)
                     /locus_tag="VFR25_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET9939724.1"
                     /translation="MTEWMGLVAGTSFAAGLNLYATVATLGLLQRFHVATLPPSLAAL
                     GHPVVIGVALVLFVVEFVADKVPFFDNVWDAVHTFIRPPAAAILAYAAVAGVHEPWRV
                     AAALIAGGVALTSHGTKASARAAVNTSPEPISNGILSTTEDVIAIGLAWLAATHPVLT
                     LVLTGILLILAILALTLIYRLLKRVFGGVRTSRPVT"
     gene            complement(7319..7717)
                     /locus_tag="VFR25_01350"
     CDS             complement(7319..7717)
                     /locus_tag="VFR25_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939725.1"
                     /translation="MFFVLLLLTLFVALATAGLVVLLFRKPIRQILDRVIGEQIAEGW
                     QRFLLFAVFVVGISNGVQIWKLEQYVHPERFGPDQAAKTLPLDGPSMGLEIYRTIIQT
                     LQGVAWALLVFFVVALIAFAIVRRGEARQG"
     gene            7841..8218
                     /locus_tag="VFR25_01355"
     CDS             7841..8218
                     /locus_tag="VFR25_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939726.1"
                     /translation="MTATPAMAHAAPCRFGRSAAILAAALLVSIAGCKGATPIHDILS
                     NAAAQDGKTVRIAGKVENSAGAFGYAVYRVDDGTGKIDVVAKTGGAPAQGTEVGVEGT
                     FRQAFTIGTDVVAVIEESKRSSR"
     gene            8372..9259
                     /locus_tag="VFR25_01360"
     CDS             8372..9259
                     /locus_tag="VFR25_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9939727.1"
                     /translation="MAETKPGIARLEIAITAILFSLGGAGIKACSLTSWQIAMFRSAI
                     AAVMFLVFLPESRRGWTRRTWIVGAGYASTMLMFVIANKLTTSANTIFLQSTAPLYVL
                     LLGPWLLRERNRTSDFLVMGLLAVGLCLFFVGHENPWRTAPDPLRGNVLAAISGVSWA
                     LTIMGMRLLGRESHGSPNPTVPAVLAGNLIAVIVSLPFAVPVPALGRVDALVLLGLGT
                     VQIGLAYVFLTRGIRSVPAFETMVILLLEPALNPVWAWLVHGERPGGWAIVGGVLILG
                     ASALKTWNDARLAPEIPPA"
     gene            9256..10311
                     /locus_tag="VFR25_01365"
     CDS             9256..10311
                     /locus_tag="VFR25_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939728.1"
                     /translation="MTRGRKALLAIFAILLAALLWLLLPTGWNPLSARARPALNRDDA
                     IERLHRLEALDDSEALYLPCRTLVLDHGARTERAYVLLHGFTNCPRQFGTLAQLLYDG
                     GANVLVPRMPRHGFANASTHVLTDLTADELIGTAGEAVDIARGLGKRVTVVGLSSSAV
                     VAAWLAQERADLDQILLIAPSFAPKDVPEAVAIPLSNALLRLPDIFVPWDPRHEWTGG
                     PKSAYSGFTAHAIARVYALGARVLESGPPAPGTRVLVVTTPHDEGVNNEVTLELARRW
                     AGRGVDVRSFEFPAGAWVHHDMIDPEQPYQRVRVVYPLLRKLLETGRIPTLADSVAYG
                     GDSTRVADAAQAGAAPP"
     gene            complement(10287..11471)
                     /locus_tag="VFR25_01370"
     CDS             complement(10287..11471)
                     /locus_tag="VFR25_01370"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9939729.1"
                     /translation="MSRPRSKRRAMLGRLILRPLLVGIPFALFFTFMQGGSLRSLPAY
                     YLVTLLFSFIIVTMVELNRAFVVPRILPPDRTPGHPLEIASFALAALLGSVIAGTILN
                     LTILHGSFGTARSVIMFLIFSVIFTTFFMALVYATRWKALLVERAEKTAREAQELRMA
                     AEIQQALLPPRRRSGAGYEAAGASIPCRTIGGDFFEYFDFTSGRIGFALGDVAGKGPP
                     AAILAAMVQGILATHANDGPAATLERVNEALCRRSIEGRFATMAYVVLDPGGTLMSCS
                     AGHNPAVLLRASGTIERPDRGGLVLGAFDTAHYEEEAIALQRGDTMVLFSDGVTDAEN
                     PAGEQYGEERLLEALAGSERRAPDEILDKVLSSVRAFADEQPPADDVTVLVIRYGGAA
                     PA"
     gene            complement(11468..11959)
                     /locus_tag="VFR25_01375"
     CDS             complement(11468..11959)
                     /locus_tag="VFR25_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9939730.1"
                     /translation="MPALAGDRAMGVRRAETAEDVEAVRVLFLEYAASLEFDLAFQDF
                     EHEVTSLPGDYAPPRGALLLATDGTAPAGCVALRPWGDEDTCEMKRLYVRPGMRGKRV
                     GVSLVAAILEEARARGYRRMRLDTVPGMDAAIALYRAAGFRDIAPYRANPVPGALFME
                     IEL"
     gene            complement(11940..12272)
                     /locus_tag="VFR25_01380"
     CDS             complement(11940..12272)
                     /locus_tag="VFR25_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HET9939731.1"
                     /translation="MSGVIYAVYTWVPEDVLAEWNAWHSGVHIPNVLKAPEMRSAVKL
                     RVTDTNLEGWRPQYVTLYTLDSLEDYAAYRDGYGLKMREEYDARYRGVGKVARVVMVE
                     ELRAGARG"
     gene            complement(12269..12691)
                     /locus_tag="VFR25_01385"
     CDS             complement(12269..12691)
                     /locus_tag="VFR25_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9939732.1"
                     /translation="MAYRFGTAEYAQALCDEINRSSEYRNAASKWGVGFNGNLLFAFE
                     ADDALRNALYLLVRLQAGRCDEAVFVPGPSHPEAGFTLRAPFGLWRDVLERRTLAATA
                     ILTGRMKVEGDKITLLKHAGSNRSLIHCTASVPTEFPG"
     gene            complement(12693..14105)
                     /locus_tag="VFR25_01390"
     CDS             complement(12693..14105)
                     /locus_tag="VFR25_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9939733.1"
                     /translation="MRPIAEGLRPLVKGRVLEPSEVEPERITNFGHIFMARPGAVLEP
                     AHAEDVAAAIAFARGEGISVSVRGAAHSQSQIAISSGGLLIDMRSMGRIGHVARGASS
                     VEVEGGAIWRDVTRTTLEHRLAPPVLTNNLSVTVGGTLSTAGVGVASHRYGTQGDQVE
                     EITVVTGTGDVRTCGPDRDPDLFDAVIAGLGQVGVITSARLKLRAVRPMTRTYYLLYD
                     DAHRFMEDTKTAMDSGRWDHLESWASPSPQGTKPVSGRRQVFARWFFPFHPTVEFDAG
                     SPPDDRALLDGLHPYDHLYTDDLPILDFFERLEPVFDLWKRGGTWGHVHPWMEVVLPW
                     DRTPDVLEAVLPDLPPAILVGGHVLLWPAKGSTSRSKLFRRPQGEDLMGFGILPAIPA
                     PFWEEAKPMLENLSRLVVAMGGNRYLSGYVDFDDEAWRTHYGDSWAFFEAAKRRHDPD
                     GILNPGFVPLRGAPASTRKG"
     gene            complement(14105..14683)
                     /locus_tag="VFR25_01395"
     CDS             complement(14105..14683)
                     /locus_tag="VFR25_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9939734.1"
                     /translation="MSTVIRSILEPAPGYASREIASFLTQMDDQTKRLTDVTRDMTPE
                     ELEWQPRPGTNTIGMLLAHMAIVEVFWTALIMKHEEHFRSEPVLGIGADDDGMPIPEE
                     GLPPAVLAGKDIAFFDDLLARGRAYVKEAAKAVPDSDLEREIPRTRADGTRRLINVRW
                     YYYHLLEHFAGHYGQILLLRHLYRAAQVPAKA"
     gene            14774..>15804
                     /locus_tag="VFR25_01400"
     CDS             14774..>15804
                     /locus_tag="VFR25_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939735.1"
                     /translation="MSFEPLPVERLYLAHELSEPIFGDTSDDVFYARRADGKRSIVRQ
                     RLGSGLAEIVTAEPRPMGGVGYGGGVFTVRGTALVYAGSDGRLHAVDLASGAQRPLTP
                     TFEGVAAPVLSPCGRAVAFLAEQDGHCNVMLADLKGKTGPIQLTDDPWYAFNPAFSPD
                     GARIAWQEWDQLDMPWDQSRIVIAKFAKPIPACSALFEAAPATVLSTIAKENASFAAP
                     QWSPDGRRFTYQSDVSGWRSLWIGDADGANAMRVDAGEGEIGLADWLPARSGYRWSDD
                     GTAIYAVRSRQSRDTLLRIDPSSRAVEEIPSAYTDIQGLQIRGHSLVYTATNPSTPQR
                     LVTRLAQGAR"
BASE COUNT         2567 a         5414 c         5334 g         2489 t
ORIGIN      
        1 gcgcccgtcc ccgatccgtc cgaccagggc gtcgtgcttc cgcacctgcg tctcgagggc
       61 cagctcggaa cgaatcccgc cgctgcccga ttccaccgag acctgcgcgc cgacgccggg
      121 cgggaaggag acggtgatgt ctccgcttcc cgattccacg acgagcgtcg agacgtcgcc
      181 ggcgaggtcg agatcgattt cccccgatcc ggtgtccatg ttgaccgact ttgcatccac
      241 gttctccaga ttgatcgagc ccgaacccgt gtcgatcgcc acggtcgccg ctttgagatt
      301 ttcgccgctc acctcgcccg agcccgtatc caccaggatg ccctccgtcg tgcgcacgtc
      361 acgcaggcgg acggccccgg agcccgtgtc gatgttgacc gcagcgtccg atccgaggat
      421 ctcgatctct ccggagccga cgtccgcgtg gaaggggcca tgcagatctt cggcgtggac
      481 cggcatggac gcgccgtcca ggaagacgtc ggcgtccacc cggctcaccg acgcgctgcc
      541 gtggccccag tagaccatca agttcgtgcc ggccgggacg aggagcttca cgtcagcgct
      601 cgcctccagc ccctcgccgc tgccggaaat ggtcatccgg tcgcccttgt cgacgccgtc
      661 cttcgaatcg ccgaaggtcc cgtcgtccct cactttgagc gtggtcctgg aattccgccc
      721 catggagcgg tcgatcacgt ggtcgtccgg atagaggacc cggagcgtct ggcggccccg
      781 gaccgctccc gtcaggacct tgagcttcgc ggcatccggc ccggcgacct cgacctccgc
      841 cgtgacggcc gagcccgttc ccggcacgat ctcgagtgat cccacgaggt tgtagacgga
      901 taccttgttg ccggtgatgg tgtaacgcac cggggcggcc tgggccctac tgatgccggc
      961 ccctgcgaga atgaggagga gtcccgcggc gcgggctccg gccaaggcac gtcgaacgct
     1021 catgcggcgc tcctttcggt atcaggatgt tgaagggacg cctgttgaga gggtcccgga
     1081 gccgaaaacg ttggggccgg gcggggacct gcccctagac ttccctacgc gccccccaac
     1141 cgcctagttt ccaaccctta ccccacccgg cctgtccaac actggcgaca ccggggcccg
     1201 aaagatccag gagtgaacgc gtcgaccaca ctcggacgag tcgtgccgca ggccatcgat
     1261 aggaccgacg tcacgcgcgc gcaggacgga gacacgcgcg ccttcgagcg gctctatcgc
     1321 agccacgtcg cgcgcgtcca cggactcgcc cgccggatga tcggccccga gcatgcggcg
     1381 gaagtcaccc aggacgtctt cgtgagggtc tgggagaagc tgggcacgtt ccgcggcgag
     1441 tcggcctttg gaacgtggct gcatcgggtc gcggtgaacg tggtcctcca ccgccgcgcc
     1501 gcgctcaagc tggagcggat gcggttcgac gatgacgagg aggcgctgga tgtcataccg
     1561 gcgccggtcc gccacgcgac agccgaattc ggcatggact tcgaccgcgc catcgacaag
     1621 ctcccggacg gcgcgcgggt ggtgttcgtg ctccatgatg tggaaggatt caagcacgag
     1681 gagatcgccg gattcctggg catcacctcg ggaacgacca aagcgcagct ccatcgagca
     1741 cggatgatcc tgcgccagca cctcacacga tgagacggaa cgaacgatga gcgaccgcta
     1801 caccgatctg atttcggatt atctcgacgg cacgctcgac ccgggaacgc accggcaggt
     1861 cgagcgccac ctcgcgggct gcaccgagtg cgccgcgctc ctcggcgact tcgagcacgt
     1921 cgtcggacgt gcccgcgttt tggccgagga ggacacgccc ccggccgagg atctctggcc
     1981 cggcatcgag gccaggctca ggcgcgccgc gcatacgccg ctcccgattc cggtcacgat
     2041 caccgcggtg ccggagcgcc gctcgctcaa accgcggatc tccttcacca ttcctcagct
     2101 cgccgccgcg tgtctcgcgg tcgtcgcgct gtcggccgct ggagcgctct acatcggtag
     2161 cgccacgcgc aaggccgaga cgaccgcctc cgtcacccag cagcttccgg cgccggccgc
     2221 ggccaccatg acggagagcc aggacgccat caaggacgtg gcgcggctca aagatattct
     2281 cgcgtcgcgc cgcaacgagc tcgatccggc gacggtcgcc gcgctcgagg ggagcctgcg
     2341 cgccatcgac aacgccatcc gccagggaca gctcgcgctg gcggcggatc ccaagaaccc
     2401 gtacttgcgc gaccatctcg acgacacgat gcgccgcaag gtggacctca tgcgccgcgc
     2461 caccatgctc gcgagcgcgg actaagcgaa ccggagaccc cccacaccat gagcctgccc
     2521 gtcgtcctcg ccgccgtcgc ggtcctgtcg ctccaagccg acgtcgacac cacgttcgcc
     2581 gtcaagaagg gggcggccct ccaggttgag aacttcgccg gcgaggtcgt cctgagcgcc
     2641 tgggacaaga acgcggtgcg cattcaggcc cctgaggccg acgacgcgcg cgtgatcgtg
     2701 aagccgacgc cccgcggcct cgtcatcaag acgggctcga agcacgtcgc ggccggatcc
     2761 gtcgatctca ggatcaccgt gcctgcgtgg atggatctcg acgtctcggg cgtccagacc
     2821 gacgtctcga tcgacggagc gcgcggacgc gtgcgggtcg cgacgatcca cggcgacatc
     2881 gcggtgtcgg gcgggcgcgg gcagatcgag ctgaacgcgg tggaccagga catccacctg
     2941 gccgacgcat cgggggtggt cgtgtccgag acggtgaacg gcgacctcac gctccagcga
     3001 atcgaatcgg acagcgtcga cgtctccacg gtgaacgggg agatcttcta cgaaggcact
     3061 ctccggagcc gaggcgtcta ccgatttacc agccacaacg gcgacatcgc ggtcgccatc
     3121 cccaccacca ccagcgccac cgtcaacgtc tcgacgttct ccggcgagtt cgcctccgac
     3181 ttccccgtca cgctccagga atcacgcccc ggccgccgtt actgcttcac gctcggcaag
     3241 ggaggcgctc gcgtcgacct cgaatccttc cagggaacga tccacgtctt ccgtcccggc
     3301 acccgagggc ccgtcaccga cgtcgagaac cagcggatgg agcggcttca ggagcgcatg
     3361 gagaaggtga aggagaagtt cgagcggcgg aagaacgagt acaagtacca gacgaagtcc
     3421 tcgaaggacg acgccgaaga tgactccgat gacgatgacg atgattcgga ggattcgaag
     3481 tagcgtctag gtcgtcgcgc gacttggccg gcgaggggag ggtgtggggg tccggaacag
     3541 cacgtccggc gctcgctatc gcacccctac cctgacttgg ttggcgccgg cgcgaatatt
     3601 ccgcaccccc acaccctccc ctcacctccc atggccccga tggactagtt gttcgcttcg
     3661 cagccaagtc gcgcgtcagg cggcggtcca gcctccgtct acgtagagct cggagccggt
     3721 gacgtacgag gactcgtcgc tcgccaggaa tgctacggcg ttggcgactt cttccgggcg
     3781 gcccaccctt cccatcgggg tgtgcgtgag gatgtagtcc agcatcggat tgggtgcgcc
     3841 ttctgacttt gcgagaaacg ggcccaccat gggggtgtcg atgaatccgg ggtggacgga
     3901 gttgacccgg attccctccc tcgcgtagcg gagggcggcg ctcttggtca tgaggcggag
     3961 ggcgcccttc gaggcatggt aggcgatggc gttgccgttg cccccgacgg ttccgtagat
     4021 cgaagccacg ttgacgatcg agcccaagcc cgcgcgcttc atggctggga cggcggcctt
     4081 cataccgagc catgcgcccg tctggttcac cgcgatcagc ttctcgtagc cctcgagcgt
     4141 ctcctcttcg acgtcctcga tgcgcgcgat gccggcattg ttgacgagca cgtggagccc
     4201 gccgaactcc cgcaccgcgg tctcgactgc cgcggtccac tcgggctcgc tcgagacgtc
     4261 gtggtgccgg tagagggccc ggccgccgga cgatcggatc gaatcggcta gggcttttcc
     4321 ctcgtcgtcg aggacgtcgg tgacgagcac cgatgcgcct tcccgcgcga gcttctcgca
     4381 gcacgcggcg ccgatccccc gcgcaccgcc ggtgacgatc gctatcctcc ctcgaagcaa
     4441 gctcatgttc gaatcctccg gtacggccgg tagacgggct cccagatcat ggcgcggatg
     4501 cgcgcctcga ccgccgcggg ttcgagctcg ggcgcagcgc cctcggatac gagcgtggtc
     4561 gccacggcca gcgcgacccg gaacgagatc tcccggagcc tcgagagcgg agggtagatg
     4621 ggcgcgcccg gccgtccgct cttgtcggtg cactcgaaga ccgcgcggct cgcggccgcg
     4681 aaggcttcgt cggtgaggcg cctggcctcg accgccacgg ctccgagccc aacgccggga
     4741 aagacgagcg cgttgttccc ctgcccgatc tcgtgaacgg tcccgccgat ctcgactggc
     4801 ggaaacgggc tccccgttcc gaccaccgcc gcacccccgg tccagccgag gagctccgcc
     4861 ggggtggcct cgcacttcga tgtcgggttg gagatcggga ggatcacagg ccgagcgcaa
     4921 tgctggaaca tcgcgcgcac gaccggctcc gtgaaggccc ccggttgccc cgacgccccg
     4981 acgagaacgg tcgggtgaaa gctggccacc acttgctcca aggtcacggg gccatctccg
     5041 acgtgacccc agcttcggac gagctcgacc ggcgcggcga tcgcggcctt ttcggcctcg
     5101 agaccggggc ggtcggccac gatgagccct ttcgaatcca gacataggac tcgtgaggag
     5161 agctcgcgag agggcacgcc ggcttccgcg agcgctcgtc gcaccgccag cgcggagccg
     5221 gctccggacg ctccggctcc gaagaagacg atgcgctcct cggcgaccgc acggcccacc
     5281 tggtgggtgg cgctccggat ccccgccgtg accaccgcac cggttccctc gatgtcgtcg
     5341 ttgaaggaag cgatttggtt tcgatgacgc gtgaggacac ggaacgcgtt ccggttcgac
     5401 aggtcctccc actggaggag cgcgcgcggg aagacgtcgc ggatcgcgtc cacgatctcg
     5461 tcgatcaagc tgtcgtactc cgggccccgc acccgccgcc ggggcacgcc gaggtagaga
     5521 ggatcgtcga ggagcgcctg attgtccgtg ccgacgtcga tgtcgagtgg caagcagcgc
     5581 gacgggtgga ggccggcccc cgcggtgtag agggcgagct ttccgatcgg gatccccatg
     5641 cccccgacgc cttggtcgcc gattccgagg atcgcctcgt tgtcggtcgc cacgatcacg
     5701 cgcacgtcgg gttgggacgc gtgccgcagg atctcgacga cccgtccccg gtcctcgatc
     5761 gacacataga ccccgcgtgg gcggcgatag atgtggctga actgctcgca caccttcccc
     5821 acggtcggcg tgtagacgat cggggccatc tcctcgagat ggtccaggag gaggcggtag
     5881 aagagcgtct cgttgcgatc ttgaagtgct gccaggaaga ggtagcgctg cacctcggag
     5941 cccgagcgga ggtagttctc gtacgcgcgg gcgcgctgct cttcctcggt cgcgaccccc
     6001 ggtgggagaa gcccgtggag atggaaggct tcccgctctt cgggtgagaa gcaggtccct
     6061 ttgttgagga ggggatcgtc cagcagcgct tccccctccc ggttcacggc ccagtagatc
     6121 tcgccggtcc gggggtcggt gcgctcttcg aacgggatca tgcgcggagt atagggtggc
     6181 tgttcggcgg tgaaacggtg gccggaagta gcatcgtctt cccggccgcg gtcagggaat
     6241 cgcgcactcc ttccctgcca tgcggcgaag caggccggcc tggccgatgt ggtacgcctc
     6301 gtggaactgg aggaagtgga gcttgtccgc gagcgtgtcg tcgccgcgct tctcgagaag
     6361 ctcctcgtcg ctcagccgcg cgagaccagc ctggatccgc tcctgcgaac gtcggaagtc
     6421 ggccacgatc aaatcgaacg ggagcgagtc cttggtgtcc tgcacgggtc gggagcctcg
     6481 cttgaagcga tcgttcctgt cggcgtccca cacttgggcc tcgcccagga gatcgagaat
     6541 cgcgccccgg ctcgccacga tgtgggccgc gacccagttg aggcagtttc ccccggccgg
     6601 cggctgcacc aggctgtcct cgtggctcag gcttttcaca ttcactccga acgcgtaggc
     6661 gtcgatctcg aagaggcgct gaagctggcg aaggctcacg acggctcctt tctcaggtga
     6721 cgggacgcga cgtgcggact ccgccgaaca cgcgtttcaa gaggcgatag atcaaggtca
     6781 gcgccaggat ggcaagaatg agcaggatcc cggtcaagac cagtgtgagc acgggatgcg
     6841 tggccgcgag ccaggcgagg ccgatcgcga tcacgtcctc cgtggtgctc aggatgccgt
     6901 tcgagatggg ctcggggctc gtgttgacgg cggcgcgcgc ggacgccttc gttccatgcg
     6961 aggtcagcgc tacgcccccc gcgatgagcg cggccgcgac gcgccagggc tcgtggacgc
     7021 ccgccaccgc ggcgtaggcg aggatcgcgg ccgccggcgg acggatgaac gtgtgaacgg
     7081 cgtcccacac gttatcgaag aagggcacct tgtccgccac gaactccacg acgaacagaa
     7141 ccagggccac gccgatcacg accggatggc cgagcgcggc gagcgacggc ggaagcgtgg
     7201 cgacgtggaa acgctgcagg aggcccagcg tcgcgacggt cgcgtagagg ttgagtccgg
     7261 ccgcgaacga ggtgcccgcg accaacccca tccattcggt catcgttcgg cccgggcgct
     7321 agccctgccg cgcttccccg cgacgcacga tggcgaacgc gatcagcgcg acgacgaaga
     7381 agacgaggag cgcccacgcc acaccctgga gcgtctggat gatggtccgg tagatctcga
     7441 gccccatgga gggtccgtcg agcggaaggg tcttcgcggc ctggtcgggg ccaaagcgct
     7501 ccggatggac gtactgctcg agcttccaga tctgcacgcc gttcgagatg ccgaccacga
     7561 acaccgcgaa caggaggaag cgttgccatc cctcggcgat ctgttcgccg atgacgcggt
     7621 ccaggatctg gcggatcggc ttccggaaga ggaggacgac gagccccgcg gtcgcgagcg
     7681 cgacgaacag cgtgaggaga aggaggacga agaacatcga ttccctttcg atgggggcct
     7741 tgggctggac gccaggcagg agtctagccg agagtcggct tcccggcgta gagttgcttc
     7801 ccagctcccg tagatcggga ggacccgaac ggaggatccc atgaccgcaa ctcccgctat
     7861 ggcgcacgcc gcaccgtgcc gattcggccg ctccgcggcc attctcgccg ccgcgctcct
     7921 cgtctcgatc gcgggctgca agggcgccac tccgatccac gatattctca gcaacgcagc
     7981 agcgcaggac ggcaagaccg tccgcatcgc cggcaaggtc gagaactccg cgggggcgtt
     8041 cggctatgcc gtctaccgtg tggacgacgg caccggcaag atcgacgtgg tggccaagac
     8101 cggcggcgcc ccagcccaag gcaccgaggt cggtgtggag ggcacgttcc gccaggcctt
     8161 cacgatcggc accgacgtag tcgccgtcat cgaagaaagc aagcgatcct cccgttgagc
     8221 atcgaggcga ttccgtgcag agggagacgt gcattgttta agccacaatg ggagggacgg
     8281 agaggcgatg accggaggac gcctcgcagt cgcgcgaagc gcgacatgcg ccgcggccgg
     8341 aggtcatcga ctctccgtcc cggccaaatg aatggctgaa acaaagcccg gcatcgccag
     8401 gctagaaatc gcaatcacag cgattctgtt ctcgctcggc ggcgcgggca tcaaggcctg
     8461 ctcgctcacg agctggcaga tcgccatgtt ccgctccgcc atcgcggccg tgatgttcct
     8521 cgtgttcctt ccggagtccc gtcgaggctg gacgcggcgg acctggatcg tgggcgcggg
     8581 ctacgcatcc acgatgctca tgttcgtgat cgcgaacaag ctcacgactt cggcgaacac
     8641 gatcttcctc cagtcgacgg cgccgctcta cgtgctgctg ctcgggccgt ggctgctccg
     8701 ggagcggaac cggacgtctg atttcctggt catgggactc ctcgccgtgg ggctctgtct
     8761 cttcttcgtc gggcacgaga acccatggcg caccgcaccc gatcccctgc gcgggaacgt
     8821 gctcgcggcc atttcgggcg tgtcctgggc gctcacgatc atgggaatga ggcttctcgg
     8881 ccgcgagagc cacggctccc cgaacccgac cgtgcccgcg gtcctcgccg gaaacctgat
     8941 cgcggtcatc gtctctcttc cgttcgcggt cccggtcccg gcgctgggcc gcgtggacgc
     9001 gctcgtcctg ctcggactcg ggacggtgca gatcggactc gcctatgtct tcctcacccg
     9061 cgggatccgg agcgttcccg cgttcgagac gatggtgatc ctgctcctcg agccggccct
     9121 caacccagta tgggcgtggc tcgtgcacgg cgagcggccg ggcggatggg cgatcgtggg
     9181 cggggttctg atcctgggag cgagcgcgct caagacctgg aacgacgccc ggctcgcccc
     9241 cgagatccct ccagcatgac gcgcgggcgg aaggcgctcc tggcgatctt cgcgatcctc
     9301 ctcgccgccc tgctctggct cctccttccc acagggtgga atccgctttc cgcgcgggcg
     9361 cgcccggccc tgaaccgcga cgacgcgatc gagaggctgc atcgcctcga agcactcgat
     9421 gattcggagg cgctctatct cccatgccgc acgctggtcc tcgaccacgg agccaggacg
     9481 gaacgcgcat acgtgctgct gcacggcttc acgaactgcc cacgccagtt cgggaccctc
     9541 gcccagctcc tctacgacgg cggcgccaac gtgctcgttc cgcgcatgcc gcggcacgga
     9601 ttcgcgaacg cgtcgaccca cgtgctcacc gatctcacgg cagacgagct gataggaacg
     9661 gcgggagaag ccgtcgacat cgcgcgcggt ctcggcaagc gcgtcacggt ggtcgggctc
     9721 tcctcgagcg ccgtggtcgc agcttggctg gcgcaagagc gcgccgatct cgaccagatc
     9781 ctcctgattg caccatcgtt cgcgccgaag gacgtgccgg aggctgtcgc gatcccgctc
     9841 tcgaacgcgc tccttcgcct tcccgacatc ttcgttccct gggatccgag gcacgagtgg
     9901 acaggcgggc cgaagtcggc gtattccggg ttcacggcgc acgcgatcgc cagggtgtac
     9961 gcactcggcg cgcgcgtcct cgagtccggt cccccggcgc cgggcacccg ggtgctcgtc
    10021 gtcacgacgc cgcacgacga aggggtgaac aacgaggtca ccctggagct ggcgaggcgg
    10081 tgggccgggc gaggtgtgga cgtgcggtcg ttcgagttcc ctgccggggc atgggtgcac
    10141 cacgacatga tcgatccgga gcagccgtac cagcgcgtga gggtcgtcta tcccctgctc
    10201 cggaagctgc tcgagaccgg gcgcatcccg acgctcgccg actcggtcgc ctacggtgga
    10261 gactcgacgc gcgtcgcgga cgcggctcag gcgggcgcag ctcctccata gcggatcacg
    10321 aggaccgtga cgtcgtccgc cggaggctgc tcgtcggcga acgcccgcac gctcgagagc
    10381 accttgtcca agatctcgtc cggagcacgt cgctcggatc cggcgagcgc ttcgagcagg
    10441 cgctcctctc catactgctc ccccgccgga ttctccgcgt ccgtcacgcc gtcgctgaag
    10501 aggaccatgg tgtctccccg ctggagcgcg atcgcttcct cttcatagtg cgccgtatcg
    10561 aacgctccga gcacgagccc gccgcggtcg ggcctctcga tcgtcccgct cgcccggagg
    10621 aggaccgccg ggttgtgtcc cgcgctgcag gacatgagcg tcccgcccgg atcgaggacc
    10681 acgtacgcca tcgtcgcgaa gcgcccttcg atggagcggc ggcagagcgc ttcattgacc
    10741 cgctccagcg tggcggccgg gccgtcgttc gcatgggtgg cgaggatgcc ctgcaccatc
    10801 gccgcgagga tcgcggcggg tggacccttg cccgcgacgt cgccgagcgc gaacccgatg
    10861 cgtcccgacg tgaagtcgaa gtactcgaag aagtcgccgc cgatcgtgcg gcacggaatc
    10921 gacgccccgg cggcctcgta gcccgcgccg ctacgccttc gcggtgggag gagcgcctgc
    10981 tggatctcgg ccgccatgcg gagctcctgt gcctcgcgag cggtcttctc ggcgcgctcc
    11041 acgaggaggg ccttccaccg ggtggcgtag acgagcgcca tgaagaacgt cgtgaagatg
    11101 acgctgaaga tcaggaacat gatgaccgac cgtgccgttc caaacgatcc gtgaaggatc
    11161 gtgaggttga ggatcgtgcc tgcgatgacg ctccccagga gagccgcgag cgcgaagctc
    11221 gcgatctcga gtgggtgccc cggagtccga tcgggcggga ggatgcgcgg cacgacgaag
    11281 gcgcgattca gctcgaccat cgtcacgatg atgaaggaaa agaggagcgt caccagatag
    11341 tacgcaggga gcgagcgaag ggatcccccc tgcatgaatg tgaagaaaag ggcgaacggg
    11401 atcccgacga gaagcggccg gaggatgaga cggccgagca tcgcgcgccg cttgcttcgc
    11461 ggccggctca cagctcgatc tccatgaaga gggcgccggg caccggattc gccctgtagg
    11521 gcgcgatgtc gcggaatccc gccgcccgat agagcgcgat ggcggcgtcc atccccggaa
    11581 cggtgtcgag acgcatccgc cggtatccgc gggctcgagc ctcttcgagg atggctgcga
    11641 cgagggacac gcccacgcgc ttcccacgca tccccgggcg gacgtagagt cgcttcatct
    11701 cgcacgtatc ctcgtcgccc cacgggcgga gcgcgacgca tccggccggc gccgtcccgt
    11761 ctgtcgcaag gagcagcgcg ccgcgcggcg gagcgtagtc ccccggcagc gacgtcacct
    11821 cgtgctcgaa atcctggaac gcgagatcga actcgagcga cgccgcgtac tcgaggaaga
    11881 gcacgcgcac ggcctcgacg tcctcggccg tctccgctcg gcgcacgccc atggcgcggt
    11941 cacccgcgag cgccggcacg aagctcctcg accatcacca cgcgcgcgac tttgcccacg
    12001 cctcggtagc gcgcgtcgta ctcctcgcgc atcttgagcc cgtagccgtc ccggtaggcc
    12061 gcgtagtcct cgagcgagtc gagcgtgtag agcgtcacgt actgagggcg ccagccctcc
    12121 agattcgtat cggtcacacg gagcttcacg gcgctccgca tctcgggcgc cttgagcacg
    12181 ttgggaatgt ggaccccgct gtgccacgcg ttccactccg cgagcacgtc ctcgggcacc
    12241 caggtgtaga cggcgtagat cacgccgctc atcccggaaa ctccgtcggc acggacgccg
    12301 tgcagtggat gagcgagcgg ttcgatcccg cgtgcttgag gagcgtgatc ttgtctccct
    12361 cgaccttcat cctgccggtc aggatcgcgg tcgcggcgag cgtgcgccgc tcgaggacgt
    12421 cgcgccagag gccgaacggc gcgcgcagcg tgaagcccgc ctcgggatgc gagggtcctg
    12481 ggacgaacac tgcctcgtcg catcgacccg cttggaggcg taccaggagg tagagcgcgt
    12541 tccggagcgc gtcgtccgcc tcgaacgcaa agaggaggtt cccgttgaac ccgacgcccc
    12601 acttggacgc cgcgttccgg tactcgctcg atcggttgat ctcgtcgcag agagcctgcg
    12661 cgtactcggc ggtgccgaag cggtaggcca tctcagccct tccgcgtcga cgcgggtgct
    12721 ccgcggagag gcacgaatcc cgggttcaga atcccatccg ggtcatggcg ccgcttcgcc
    12781 gcttcgaaaa aggcccacga gtccccgtag tgcgtccgcc acgcttcgtc gtcgaagtcg
    12841 acgtagcccg agaggtagcg attccctccc atcgcgacga cgagccggct caagttctcg
    12901 agcatcggct tggcttcctc ccagaacggc gcgggaatcg cgggaaggat gccgaacccc
    12961 atcaagtcct ccccttgggg acggcggaag agcttcgatc gcgacgtgct ccccttggcg
    13021 ggccagagga gcacgtgccc gcccacgagg atggccggcg gaagatcggg gagcacggcc
    13081 tcaagcacgt cgggggtccg atcccacggc agcacgacct ccatccaggg atgcacgtgc
    13141 ccccaggtgc cgccgcgctt ccagaggtcg aacacgggct ccagccgctc gaagaaatcg
    13201 aggatgggga ggtcgtctgt gtagaggtga tcgtagggat gcagcccgtc gaggagcgca
    13261 cggtcgtccg gaggcgatcc cgcgtcgaac tccacggtcg gatggaacgg gaagaaccag
    13321 cgcgcgaaca cctgccgccg cccgctcacc ggcttcgtac cctgcgggga tggagacgcc
    13381 cagctctcga gatggtccca gcgtccggag tccatcgcgg tcttcgtgtc ttccatgaag
    13441 cggtgcgcgt cgtcgtagag gagatagtag gtgcgggtca tcgggcggac ggcgcggagc
    13501 ttcaagcgcg cgctcgtgat gacgcccacc tggcccagtc cggcgatcac cgcgtcgaag
    13561 agatccggat cgcgatcggg gccgcacgtg cggacgtcgc ctgtgcccgt gacgacggtg
    13621 atctcctcga cctggtcgcc ttgggtgccg tagcggtgcg acgcgacgcc gaccccggcc
    13681 gtggagaggg tgccccccac ggtgacgctc aagttgttcg tcaggaccgg cggtgcgagg
    13741 cggtgctcga gcgtggtccg cgtcacgtcg cgccagatcg cgcccccctc gacttcgacg
    13801 ctcgacgcgc ctctcgctac atgcccgatc cgccccatgg aacgcatgtc gatgaggagg
    13861 ccgccgctcg agatcgcgat ctggctctgg ctgtgcgcgg cgccgcgcac cgagaccgag
    13921 attccctcgc cgcgcgcgaa ggcgatcgca gcggccacgt cctcggcgtg cgcgggctcg
    13981 agcaccgcgc ccggccgcgc catgaagatg tggccgaagt tggtgatccg ctccggctcg
    14041 acctcggagg gttcgaggac ccggcccttc acgagcggcc ggagtccttc cgcgatggga
    14101 cgcactaggc cttggccggt acctgggccg cgcgatacag atgccggagc agcaggatct
    14161 gcccgtagtg acccgcgaag tgctcgagga ggtggtagta gtaccaccgc acgttgatga
    14221 gccggcgggt gccgtccgca cgggtgcgcg ggatctcgcg ctcgaggtcc gagtcgggca
    14281 ccgccttggc ggcttccttc acgtatgcac ggccgcgcgc gagcagatcg tcgaagaacg
    14341 cgatgtcctt ccccgcaagg accgcggggg gcaggccctc ctcgggaatg ggcatgccgt
    14401 cgtcgtcggc gccaatgccg agcacgggct cgctccggaa gtgctcctca tgcttcatga
    14461 tgagcgcggt ccagaacacc tccacgatcg ccatgtgcgc gagcagcatc ccgatcgtgt
    14521 tcgtgccggg acgcggctgc cactcgagct cctcgggggt catgtcccgc gtgacgtcgg
    14581 tgaggcgctt cgtctggtcg tccatctgcg tgaggaacga cgcgatctcc cgggaggcgt
    14641 agccgggcgc gggctcgagg atggaacgga tcacggtcga catggtgatg cctccggagc
    14701 ggaatcgggt ggatggtcgt cgggaccggg aaagcatagc agtcccaagc cgcggggggt
    14761 atcatcgccc gccatgtcct tcgagccgct tccggtcgag cgcctctacc tcgcgcatga
    14821 gctgagcgag cccatcttcg gcgacacgtc cgacgatgtc ttctacgcgc ggcgcgccga
    14881 cgggaagcgg agcatcgtgc gccagcgcct tggaagcggc ctcgccgaga tcgtgacggc
    14941 cgagccgcgt cccatgggag gcgtcgggta cggcggcggc gtcttcacgg tccgcggcac
    15001 ggcgctcgtc tacgcgggga gcgatgggcg cctccacgcg gtggacctcg cctcgggcgc
    15061 ccagcgaccc ctgaccccga ccttcgaggg agtggccgcg ccggtcctct cgccgtgcgg
    15121 acgagcggtc gcgtttctcg ccgaacagga cgggcactgc aacgtgatgc tcgccgacct
    15181 gaagggcaag acgggtccga tccagctcac ggacgatccc tggtacgcgt tcaatcccgc
    15241 gttttcgccc gacggcgcgc ggatcgcgtg gcaggagtgg gatcaactcg acatgccgtg
    15301 ggatcagtcc cggatcgtga tcgcgaagtt cgcgaagccg atccccgcgt gctcggccct
    15361 cttcgaggcg gctccggcca cggtcctctc gaccatcgcc aaggagaacg cgagctttgc
    15421 cgcccctcag tggagccccg acggaaggcg cttcacctac cagagcgacg tctcgggctg
    15481 gcgatcgcta tggatcggcg acgcagacgg cgcgaacgcg atgcgtgtgg acgcgggcga
    15541 aggagagatc ggcctcgcgg actggctgcc ggcgcgctcc ggctatcggt ggagcgacga
    15601 cgggacggcg atctatgcgg tgcggagccg ccagagccgg gacacgctgc tccggatcga
    15661 tccatcgtcg cgtgcggtcg aggagatccc gtcggcgtac accgatatcc agggactcca
    15721 gattcgcggg cactcgctcg tgtacaccgc gacgaaccca tcgacgcccc agcgtctcgt
    15781 gacccggcta gcccagggcg cccg
//
LOCUS       DASTKC010000036         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1918649, whole genome shotgun sequence.
ACCESSION   DASTKC010000036 DASTKC010000000
VERSION     DASTKC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918649"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFR25_01405"
     CDS             <1..591
                     /locus_tag="VFR25_01405"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HET9939736.1"
                     /translation="NMGTIPLHVWSARVASLDRPDWMVLDLDPKEAPFTHVVQVARAL
                     KKILDDLELPSVPKTSGATGLHVLLPMGQRYTHEEVRTFARVLATMVVEALPEISTIV
                     RAIRGRGGKVYVDFGQNGHGNTIVAPYSVRPLPGAPASCPLRWDEVNARLDPARFTIR
                     TIPERFEEMEDPLRMVLGEGIDMAEAIATIERRMRG"
     gene            584..1279
                     /locus_tag="VFR25_01410"
     CDS             584..1279
                     /locus_tag="VFR25_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET9939737.1"
                     /translation="MADQGTATTTELRFLASSSAGEVSAILIRPAGARWLYVFGHGAG
                     AGMRHHFMEDAATALAGRGFATFRYQFAYQEKGSRRIDPQPILLATVRAAIDAARQAA
                     PDLPLLAGGKSMGGRMTSLAASKEPLHGVKGIAFFGFPLHPAGAPDQVRADHLALVTV
                     PMLFLQGTRDTLANLDLLRPVIDKLGAPATLHVLDGADHGFNVLKSSGRTPADVMEEL
                     TRTMEGWAESLPR"
     gene            1299..1991
                     /locus_tag="VFR25_01415"
     CDS             1299..1991
                     /locus_tag="VFR25_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9939738.1"
                     /translation="MRIRAGTSGWSYKEWKGHFYPEKLAAKDMLRYYAEHFPTVEVNN
                     TFYRMPNLTTLEGWAAEVPEDFSFVLKATKRITHDKRLKDVQDSVDYLLRTAGTLGSK
                     LGPFLVQLPPNMKKDVPRLRDFLAGLTARAAFEFRHSSWHDDEVYSALRERNMAWCIA
                     DTGEPDDPPFVSTADWGYFRLRRVVYEDADLKTWADRVRGETWKEAFVFFKHEDAGTG
                     PKLATRFLELCR"
     gene            2031..2651
                     /locus_tag="VFR25_01420"
     CDS             2031..2651
                     /locus_tag="VFR25_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939739.1"
                     /translation="MNNADSQTVFLLSPAHCGGRRAQLLLREGAAFPLAVRMRETGAP
                     IGEVFAFLSGLYFRGKLAYARAFARRSTPPGVLVITPGLGLIDPDRVVTLEDLRAIAA
                     VGVDEDEPRFRAPLEAHAADLAASLAGDDRVVLLGSIATGKYVDVIERHLDGRLRFPA
                     DFVGRGDMSRGGLMLRCVESRQELTYVPIAGAVRHGPRPPKLEPLR"
     gene            2648..>3581
                     /gene="ligD"
                     /locus_tag="VFR25_01425"
     CDS             2648..>3581
                     /gene="ligD"
                     /locus_tag="VFR25_01425"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9939740.1"
                     /translation="MSPAKATTRKKGSAPKTPTARTKGTPRTKGIARTKTAAITIPTG
                     VRDVEIDFDGRVVRLTNLDKPFWPELGITKRDLLQYYARVAPVLLPHLKDRAMVMKRY
                     PNGAHGEFFFMKRAPSPRPEWIETCRIPHGSGNVIDFPIIQDLPSLLWVINLGCIDLN
                     PWYARCDDTDRPDFLHFDLDPVPKATFGQVIETALLVRRGLEGLGMECHAKTTGSKGI
                     HVYVPIKRGPVQKEVWTFAKNFAFGLAAHAPKLVTAEYRIAKRPADHVLVDYNQNAWG
                     RTLASVYSVRPRDRATVSTPVTWEELERGVEIEDF"
BASE COUNT          583 a         1228 c         1198 g          572 t
ORIGIN      
        1 aacatgggca cgatcccgct ccacgtctgg agcgcgcgcg tggcgtcgct cgaccggccc
       61 gactggatgg tgctggacct cgatcccaag gaagctccat tcacccacgt cgtccaggtc
      121 gcacgcgcgc tcaagaagat cctcgatgac ctcgagctgc cgagcgttcc caagacgtcg
      181 ggcgcgaccg gcctgcacgt cctgctcccc atggggcagc gctacacgca cgaggaggtg
      241 cgcacgttcg cccgcgttct cgctacgatg gtggtggagg cgcttcccga gatctcgacc
      301 atcgtacggg cgatccgggg gcgcggcgga aaggtgtacg tcgacttcgg acagaacggg
      361 catggcaata ccatcgtcgc cccgtactcg gtccggccgc ttccgggcgc tccggcgtcg
      421 tgcccgcttc gatgggatga ggtgaacgcg cggctcgacc ccgcgcgctt cacgatccgg
      481 acgatccccg agcggttcga ggagatggaa gatccgctcc ggatggttct gggcgagggc
      541 atcgacatgg ccgaggcgat cgcgacgatc gagcggagga tgcgtggctg accaaggcac
      601 cgcgaccacg acggagcttc gattcctcgc cagctcctcg gccggcgagg tctccgcgat
      661 cctcatccgg ccggcgggcg cgcgctggct ctacgtcttt ggccacggcg cgggcgcggg
      721 gatgcggcat cacttcatgg aggatgcggc caccgccctc gcggggcgcg gcttcgcgac
      781 ctttcgctat cagtttgcct accaggagaa gggatcgcgc cgcatcgatc cgcagcccat
      841 cctcctcgcc accgttcgag ccgcgatcga tgcggcccgc caagccgcgc ccgacttgcc
      901 gctgctcgct ggaggcaagt cgatgggagg ccgcatgacc tctctcgcag cgtccaagga
      961 gccgctccac ggggtgaagg ggatcgcgtt ctttggattc cccctccatc ccgcgggggc
     1021 gccggaccag gtgcgcgccg atcatttagc gctggtcacg gtccccatgc tcttcctcca
     1081 gggcacgcgg gacacgctcg ccaacctcga ccttctccgg cctgtcatcg acaagctcgg
     1141 cgccccggcg acgctccacg tcctggacgg cgccgatcac ggcttcaacg tgctgaagag
     1201 ctccggccga actccggctg acgtgatgga ggagctgacg cgtaccatgg aagggtgggc
     1261 ggagagcctg ccccggtagc ctggacccag gagcacgcat gcggatacga gcgggaacga
     1321 gcggctggtc gtacaaggag tggaagggac acttctatcc cgagaagctc gcggcgaagg
     1381 acatgctccg ctactacgcc gagcactttc ccacggtcga ggtgaacaac acgttctatc
     1441 gcatgccgaa cctcacgacg ctcgagggct gggccgccga ggtgcccgag gatttttcat
     1501 tcgtcctcaa ggcgacgaag cggatcacgc acgacaagcg cctcaaggac gtccaggact
     1561 ccgtggacta tctcctccga accgcgggca cgctcggatc gaagctcggg ccgttcctcg
     1621 tccagctgcc gccgaacatg aagaaggacg tgccgcgcct gcgcgacttc ctcgcggggc
     1681 tgacagcccg cgccgcattc gagttccgcc attcgtcctg gcacgacgac gaggtgtact
     1741 cggcgctccg cgagcgcaac atggcctggt gcatcgccga tacgggcgag ccggacgatc
     1801 ctcccttcgt gtccactgcg gattggggct acttccggct ccgtcgcgtc gtgtatgagg
     1861 acgccgatct caagacatgg gcggatcgcg tgcgaggcga gacgtggaag gaggcgttcg
     1921 tcttcttcaa gcacgaggac gccgggaccg gccccaagct cgcgacccgc tttctggagc
     1981 tgtgccgtta ggcgcgccgt cacaagggcc aaggggaaga agctagagcg atgaacaacg
     2041 cggattcgca gaccgtattc ctgctctcgc cagcccactg cggtggtcga cgggcccagc
     2101 tcctgcttcg cgagggggcg gccttcccgc tcgcggtgcg tatgcgggag accggcgccc
     2161 ccatcggaga ggtgttcgcg tttctgagcg ggctctattt ccgggggaag ctcgcgtatg
     2221 cgcgcgcctt cgcgcgccgc tcgacgccgc ccggcgtgct cgtcatcacg ccggggctgg
     2281 ggctgatcga tcccgatcgc gtcgtgacgc tcgaggatct ccgggcgatc gccgcggtgg
     2341 gtgtggacga ggacgagccg cgattccgcg ccccgctcga agcgcacgcc gccgacctcg
     2401 cagcgtcgct cgcaggcgat gaccgcgtgg tcctgctcgg gagcatcgcg acggggaagt
     2461 acgtggatgt gatcgagcgg cacctggacg gccgtctccg atttccggcc gacttcgtgg
     2521 ggcgcggcga catgagccgg ggcggactca tgctgcgctg cgtcgagagc cgccaggagc
     2581 tcacgtacgt gcccatcgcc ggcgcggtgc gccacgggcc ccggccgccc aagctggagc
     2641 cgctccgttg agcccggcca aggcgactac gcggaagaag gggtccgcgc cgaagactcc
     2701 gaccgcgcgg acgaagggga ccccgcggac gaaggggatc gcgcggacga agacggccgc
     2761 catcaccatc ccgaccggcg tccgcgacgt cgagatcgac ttcgacggcc gggtcgtccg
     2821 cctcaccaac ctcgacaagc ccttctggcc cgagctcggg atcacgaagc gcgatctcct
     2881 ccagtactac gcccgcgtcg cgccggtgct cctgccgcat ctcaaggacc gcgcgatggt
     2941 gatgaagcgc tatccgaacg gcgcacacgg tgagttcttc ttcatgaagc gcgcaccgtc
     3001 gcctcggccg gagtggatcg agacctgccg catcccgcac ggctcgggga acgtcatcga
     3061 ctttccgatc atccaggacc tgccgtcgct cctctgggtg atcaacctcg gctgcatcga
     3121 tctcaatccc tggtacgcgc gctgcgacga caccgatcgt cccgacttcc tccatttcga
     3181 cctcgacccc gtgcccaagg cgacgttcgg ccaggtgatc gagaccgcgc tcctcgtgcg
     3241 gcgcgggctc gagggcctgg gcatggagtg ccatgcgaag acgaccggat cgaagggcat
     3301 ccatgtctac gttcccatca agcgtggacc ggtccagaag gaagtctgga cgttcgcgaa
     3361 gaatttcgcg tttggtctcg ccgcgcacgc gccgaaactg gtgaccgcgg aataccggat
     3421 cgccaagcgt ccggcggacc acgtcctcgt ggactacaac cagaacgcct gggggcgcac
     3481 gctcgcctcg gtctactcgg tgcgcccgcg cgaccgggcg accgtgtcca cgccggtcac
     3541 gtgggaggag ctggagcgcg gggtcgagat cgaggacttc a
//
LOCUS       DASTKC010000037         6433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_263855, whole genome shotgun sequence.
ACCESSION   DASTKC010000037 DASTKC010000000
VERSION     DASTKC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263855"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFR25_01430"
     CDS             <1..911
                     /locus_tag="VFR25_01430"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9939741.1"
                     /translation="LIGEDIASYGGVFKATRGLYEKFGPMRVIDSPISENFLVQGMVG
                     AAIAGLIPSPEIQFDDFISLAVDGIVEHAAKMRYRSGGMFTCPMVIRCCCGAAVGGGL
                     FHSQFNASWFMHAPGLVVVAPSNPHDAKGLLKSAFRGQNPVLYYEHKRLYRTVKGPVP
                     QHDYTVPLGKAAVSREGRHVTVISYSLMHQRSLEAAEKLAAEGIDVEVIDLRTLAPYD
                     KETILASVEKTARAVVVYESSRTMGVGAEIGAMIAEEGFSFLDAPVRRVSPPDAPQEP
                     FAPVLADHYLPDANRIAQAIRETVAY"
     gene            942..2315
                     /gene="sucB"
                     /locus_tag="VFR25_01435"
     CDS             942..2315
                     /gene="sucB"
                     /locus_tag="VFR25_01435"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET9939742.1"
                     /translation="MPVTIVVPQLGESVVEGTIGKWLKKVGDPIAKDEPIVEIITDKI
                     NIELPSPAAGTLGQIAVPEGQVAQVGDPLGQILAAGESASAAAPAAPKPAAAGSPAPQ
                     AASQAAALAPQAPAPSRPAPSPAPAYATAVAEPPSTDGQRLSPAVRKLAKEHQVDTAQ
                     IRGSGMGGRVTREDVLTYLEARKVAASAPSFAPAGAPGRAPESTPQPKPLKPIPSAAF
                     AAAGARESERKPLTNIRKKIAENMVKSRHTAAHCSTWDEVDMTNLVELRGRLKDRVRM
                     EYGIGLTYMPFIMRAVVRALKEYPILNASMTDTEVELKKYYNIGVAVHRDQGLIVPVV
                     HDADRKTLLQLAAEIDDLGRRARNDKLTLDDIQGGTFTITNAGMFGATASTPIINVPE
                     VGILGVHLIQERAVVRDHRIVVRWMMNLVISFDHRLVDGTPAVQFLHRVKELLEDPES
                     WLLDSVG"
     gene            2312..3028
                     /gene="lipB"
                     /locus_tag="VFR25_01440"
     CDS             2312..3028
                     /gene="lipB"
                     /locus_tag="VFR25_01440"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET9939743.1"
                     /translation="MTSKAPIPLYDLGRVPYRAALSFQHRAVETRARGESPDVLYTME
                     HEPVLTLGRSTEPGTLKASAAELARRGIEVIPVERGGDVTYHGPGQIGGYPIVDLAGL
                     PGGRDLHRYLRDLEEALIRTLASYGLTAERRPPYTGVWVGDRKVAAIGVAVRRWVAFH
                     GFALNVDPDLTHFDLIHPCGIRHLGVASMASLLGQAPPRDQVLDRLASAFARVWDRPV
                     ATAGLEASPSIPLPEESSHV"
     gene            3021..4460
                     /locus_tag="VFR25_01445"
     CDS             3021..4460
                     /locus_tag="VFR25_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HET9939744.1"
                     /translation="MSDAVKEPPAASRPGSTRTERDSLGPLEVPADAYYGVQTARAIA
                     NFPISGERLHPEMVRAVTTIKLAAAKANAELGVLDAKKAEAIAVAAREVIEGRLADQF
                     VVDAYQAGAGTSFHMNVNEVLANRACEILGGARGDQKLVNPNDHVNMAQSTNDVIPTA
                     MRIAAYSLSGPLLEELDLLTQSLDRKAKEFARVLKSGRTHLQDAVPVTLGQEFSGYRE
                     CVAGWAASLRKSREGLLALGLGGNAAGTGINAHPKYRERVVATIAEITGSPYRSSPNL
                     FEAMQSMAPFVRVSNDLRGFALDLNRIANDLRLLASGPTTGFDEVVLPAVQPGSSIMP
                     GKVNPVIAEMTNMVCYQVLGYDTTVAYAAQAGQLELNVMMPVIAHNLLRSIALLTPTL
                     RVLRTTCIDGITANEDVCRRYFDRSISLATALNPYIGYARAAEVAKESAKTGKTVIEI
                     VREQKLLTEEQIAQVFSPENMTHSEGGQS"
     gene            4457..5146
                     /locus_tag="VFR25_01450"
     CDS             4457..5146
                     /locus_tag="VFR25_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9939745.1"
                     /translation="MKRFPLAWIVLAALVIATPAFAGTAQFGIGVFGGYNTYSMNDVN
                     DDITSTNDTYGTAIDEMNGGVGFGGGLRIRTSPALSFALDYERLNSSTSTSGTDGTTT
                     FDVNFDLPANAFVASATYLFPSASKARFGVTGGIGYYKADGSLELTVDDGVTPITLSG
                     DASGSGVGFHGGGSLDVSLSPVAHFEAMAGYRVAKTGNLEVDGTEIPDYAAEYSGFMS
                     RAGFSFFFGSH"
     gene            5217..5942
                     /locus_tag="VFR25_01455"
     CDS             5217..5942
                     /locus_tag="VFR25_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939746.1"
                     /translation="MIQYVRAAGWTAFVAAVLLLPVPARSSTRVALAIHAGYNEYDMT
                     DVNQDLITHSINELILGGSGFALEEIHHGLGFGAGLRFHASKRFSFALDYDRLTAKSH
                     LSLFNIDFNVKAPAHSVTGSAIYWFPSATRVRGGVGAGGGYYWSNGEFGADTSGVGVT
                     TPMTGSGPGFHGMGLFDYTISPKVHFEANAGLRWAETKDLEIEGAKAVTSDGSNATLD
                     WSGFMSRIGLTFYFGPGEAVPGS"
     gene            complement(5939..>6433)
                     /locus_tag="VFR25_01460"
     CDS             complement(5939..>6433)
                     /locus_tag="VFR25_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939747.1"
                     /translation="VAAEYQTVYVPFNEEKPFQQALMLTVRLQGPGGVKAEVGSYIDP
                     TGRVRYTAQGSQFVYRGSSAAPKVPEFGIPDNVVKGTVQDDVGSPISGAALRIGDDLV
                     FTDSQGAFFVRTKKARDYPLTILFDEFLGPSRYEGLFVPGQVTAQPEARATPISIVLR
                     RVAN"
BASE COUNT         1045 a         2233 c         2139 g         1016 t
ORIGIN      
        1 tcctgatcgg cgaggacatt gcgagctacg gcggcgtctt caaggcgacg cgcgggctct
       61 acgagaagtt cgggccgatg cgcgtcatcg acagcccgat ctccgagaac ttcctggtcc
      121 aggggatggt gggcgcggcg atcgccggtc tcattccctc ccccgagatc cagttcgacg
      181 acttcatctc gctcgcggtg gacgggatcg tcgagcacgc cgcgaagatg cggtatcgct
      241 ccggcggcat gttcacctgc ccgatggtga tccgctgctg ctgcggcgcc gccgtcggcg
      301 gcgggctctt ccactcgcag ttcaacgcgt cctggttcat gcacgcgcct ggcctcgtcg
      361 tggtcgcgcc gtccaatccg cacgacgcga agggactctt gaaatccgcg ttccgcgggc
      421 agaacccggt cctctactac gagcacaagc gcctctaccg gacggtgaag ggaccggtgc
      481 cgcagcacga ctacacggtc ccgctcggca aggccgcggt gtcgcgcgag ggacgccacg
      541 tcacggtgat ctcctattcg ctcatgcacc agcgctcgct cgaggcggcg gagaagctcg
      601 cggcggaagg gatcgacgtc gaggtcatcg acctgcgcac gctcgctcct tacgacaagg
      661 agacgatcct cgcgtcggtc gagaagaccg cgcgcgccgt ggtcgtctac gagagctcgc
      721 gcacgatggg cgtcggcgcc gagatcggcg ccatgatcgc cgaggagggc ttctcgttcc
      781 tcgacgctcc ggtccgccgc gtgtcgccgc ccgacgcgcc gcaggagccg ttcgcgccgg
      841 tgctcgccga ccactatctg cccgatgcca atcggatcgc ccaggcgatc cgcgaaacgg
      901 tcgcgtacta gccgggccca gagcccgcac gggaggaagg gatgccggtc accatcgtag
      961 tgccccagct cggcgagagc gtggtcgagg gcacgattgg gaagtggctg aagaaggtcg
     1021 gcgatccgat cgcgaaggac gagccgatcg tcgagatcat caccgacaag atcaacatcg
     1081 agctcccgtc ccccgccgcc ggcacactgg gacagatcgc ggtgcccgag ggacaggtcg
     1141 cccaggtcgg cgatcctctc ggccagatcc tcgccgcggg cgagtccgcg tcggcggccg
     1201 ctccggcggc gcccaagcct gctgccgcgg gtagtcccgc gccgcaggcg gcgtcgcaag
     1261 ccgcggcctt agcacctcag gcgccagcgc cttcgcgtcc cgccccttca ccggcccccg
     1321 cgtacgccac ggccgtggcg gagccgccgt ccaccgacgg tcagcggctc tcccccgcgg
     1381 tgcggaagct cgcgaaggag caccaggtcg acaccgcgca gatccgcggc tcgggcatgg
     1441 gcgggcgagt cacgcgcgag gacgtcctta cctatctcga agcgcgcaag gtcgccgcgt
     1501 cggcgccctc cttcgcaccg gccggtgcgc cgggccgcgc tcctgagtca acgccgcagc
     1561 cgaagccttt gaagccgatc ccctccgccg cgttcgcggc ggccggagcg cgcgagtcgg
     1621 agcggaagcc gctcacgaac atccggaaga agatcgccga gaacatggtg aagtcgcgcc
     1681 acaccgcggc ccactgctcc acgtgggacg aggtggacat gacgaacctc gtcgagctcc
     1741 gcggtcggct caaagaccgg gtgaggatgg agtacgggat cgggctcacg tacatgccgt
     1801 tcatcatgcg ggcggtggtg cgcgcgctga aggagtaccc gatcctgaac gcctcgatga
     1861 ccgacaccga ggtggagctc aagaagtact acaacatcgg cgtcgccgtg caccgcgacc
     1921 aggggctcat cgtcccggtc gtccacgacg cggatcggaa gaccctcctt cagctcgcgg
     1981 ccgagatcga cgacctgggc cggcgcgcgc ggaacgacaa gctcacgctc gacgacatcc
     2041 agggcgggac cttcacgatc acgaacgcgg gcatgttcgg cgccacggcg tcgaccccca
     2101 tcatcaacgt ccccgaggtc ggcatcctcg gcgtgcacct gatccaggag cgtgcggtgg
     2161 tgcgggatca tcggatcgtg gtccgctgga tgatgaacct cgtgatctcc ttcgatcacc
     2221 gtctcgtcga cggcacaccg gcggttcagt tcctgcaccg cgtgaaggag ctcctggagg
     2281 atccggagag ctggctcctg gattcggtgg gatgacctcg aaggcgccca ttccgttgta
     2341 cgacctgggc cgcgtgccat accgcgcggc cctttcgttt cagcatcgcg ccgtggagac
     2401 gcgcgcgcgc ggcgaatcgc ccgacgtgct gtacacgatg gagcacgagc ccgtgctcac
     2461 gctggggcgg tcgaccgagc cgggcacgct caaggcgagc gccgccgagc tcgcgcgtcg
     2521 cggcatcgaa gtgatcccgg tcgagcgggg cggcgacgtg acgtatcacg gccccgggca
     2581 gatcgggggc tatccgatcg tcgatctcgc ggggctcccg ggaggacgcg atctccaccg
     2641 ctacctgcgc gacctcgaag aggccctcat ccgcacgctc gcaagctacg gcctcaccgc
     2701 ggaacggcgc cctccctaca ccggcgtctg ggtcggcgac cggaaagtcg cggcgatcgg
     2761 cgtcgcggtg cggcgatggg tcgcgttcca cggcttcgcc ttgaacgtcg atcccgatct
     2821 cacgcacttc gatctcatcc atccctgcgg catccgccat ttgggcgtcg cctccatggc
     2881 gtcgctcctg ggccaggcac cgccgcgcga ccaggttctc gaccgtctcg cgagcgcgtt
     2941 cgcgcgcgtg tgggaccgtc ccgtcgccac cgcgggcctc gaggcgtccc ccagcatccc
     3001 tttgccagag gagtcgtccc atgtctgacg ctgtgaagga gcctcccgcg gcctcccgtc
     3061 ccggctccac gcgcaccgag cgcgattcgc tcgggccgct cgaggtgccg gccgacgctt
     3121 actacggcgt ccagacggcg cgcgcgatcg ccaacttccc gatctccggc gagcgcctcc
     3181 atcccgagat ggttcgcgcc gtcacgacga tcaagctcgc cgccgccaag gcgaacgccg
     3241 agctcggcgt gctcgacgcg aagaaggccg aggcgatcgc ggtcgcggcg cgcgaggtga
     3301 tcgagggccg cctcgccgac cagttcgtgg tggacgcgta ccaggcgggc gccgggacct
     3361 cgttccacat gaacgtgaac gaggtgctcg cgaatcgcgc ctgcgagatc ttgggcggcg
     3421 cgcgcgggga ccagaagctc gtgaacccga acgatcacgt gaacatggcg cagtcgacca
     3481 acgacgtgat cccgaccgcg atgcggatcg cggcctactc cctctccggg ccgctcctcg
     3541 aggagctcga cctcctcacc cagtcgctgg atcggaaggc gaaggagttc gctcgcgtct
     3601 tgaagtcggg gcgcacgcat ctccaggacg cggttcccgt gaccctcggg caggagttct
     3661 cgggctaccg ggagtgcgtg gccggatggg ccgcgtcgct tcggaagagc cgcgaaggcc
     3721 tcctcgcgct cgggttgggc ggaaacgcgg ccggcaccgg catcaacgcg cacccgaagt
     3781 accgggagcg cgtcgtcgcg acgatcgccg agatcacggg gagtccctat cgctcgtcgc
     3841 ccaatctctt cgaggcgatg cagagcatgg cgccgttcgt ccgcgtgtcg aacgatctgc
     3901 gagggttcgc gctcgatctc aaccggatcg cgaacgacct gcggcttctc gcgagcgggc
     3961 cgacgacggg cttcgacgag gtcgtgctcc ccgcggtgca gccggggtcg tcgatcatgc
     4021 cgggcaaggt caatccggtg atcgccgaga tgacgaacat ggtctgctac caggtcctgg
     4081 gctacgatac gaccgtcgcc tacgcggcgc aggccgggca gctggagctc aacgtgatga
     4141 tgcccgtgat cgcgcacaac ctgctccgct cgattgcgct tctcaccccc accctgcgcg
     4201 tgctccgcac cacgtgcatc gatgggatca cagccaacga ggacgtgtgc cggcgctact
     4261 tcgaccgctc catctcgctg gcgaccgccc tcaacccgta catcggctat gcacgggccg
     4321 cggaggtggc caaggagtcg gccaagacgg gaaagacagt gatcgagatc gtgcgggaac
     4381 agaagctgct cacggaggag cagatcgcgc aggtcttctc cccggagaac atgacccact
     4441 cggaaggagg gcaatcgtga agcgctttcc tctcgcctgg atcgtcctgg cggcgctcgt
     4501 catcgccacc cccgccttcg ccggaaccgc gcagttcggg atcggcgtgt tcggcgggta
     4561 caacacgtat tccatgaacg acgtgaacga cgacattacc agcacgaacg acacctacgg
     4621 aacggccatc gacgagatga acgggggcgt cggcttcgga ggcggcctcc gcatccgcac
     4681 ctccccggcg ctcagcttcg cgctcgacta cgagcgtttg aacagcagca cgagcacgtc
     4741 cggtaccgat ggcaccacca ccttcgatgt caatttcgat ttgccggcga acgcgttcgt
     4801 cgcgagcgcc acgtacctct tcccctcggc ttccaaggcg cgcttcggcg tgaccggcgg
     4861 catcgggtac tacaaggccg acggcagcct ggaactgacg gtggatgatg gagtgacccc
     4921 catcacgctg tcgggggacg cgtccgggag cggcgtcggc ttccatggcg gcggttcgct
     4981 cgacgtttcg ttgagcccgg tcgctcattt cgaggcgatg gcgggatacc gggttgccaa
     5041 gaccggcaac ctcgaggtcg acggcacgga gatcccggat tacgcggcgg aatacagcgg
     5101 cttcatgagc cgcgccgggt tctcgttctt cttcggcagt cattgactca cgcggggctc
     5161 cgcctcgtgg cggggccccg ttccccttct tggccggcgc agcacgagga gcgatcatga
     5221 tccaatacgt gcgggccgcc ggctggacgg cattcgtcgc cgccgtcctc ctgcttccag
     5281 tcccagcgag atcatcgacg cgggtcgcgc tcgcgatcca cgcgggctac aacgagtacg
     5341 acatgacgga cgtgaaccag gatctcatca cccattcgat caacgagctg atcctgggag
     5401 gctccggatt cgccctcgag gagatccacc acggcctcgg cttcggcgcc ggactccgct
     5461 tccacgcgtc gaagcgcttt tcgttcgccc tggactacga tcgcctgacc gcgaagtcgc
     5521 acctctcgct cttcaacatc gacttcaacg tcaaggcgcc cgcgcactcc gtgaccggga
     5581 gcgcgatcta ctggttcccg agcgcgacgc gcgtgcgcgg cggcgtgggc gccggaggcg
     5641 gctactactg gtcgaacgga gagttcggcg cggacacgtc gggggtggga gtcacgactc
     5701 cgatgacggg gagcgggcct ggctttcacg gcatggggct cttcgactac accatctccc
     5761 ccaaggtgca cttcgaggcg aacgcgggcc ttcgctgggc ggagaccaag gatctcgaga
     5821 tcgaaggagc caaggccgtg acgtcggacg ggagcaacgc cacgctggac tggagcggct
     5881 tcatgagccg catcgggctg accttctact tcggcccggg cgaggcggtc cccgggagtt
     5941 agttcgcgac ccggcgcagc acgatcgaga tcggcgtggc gcgcgcctcg ggctgagccg
     6001 tcacctggcc gggcacgaac agtccctcgt agcgcgaggg gcccaggaac tcgtcgaaga
     6061 ggatcgtgag cgggtagtcc ctcgccttct tcgtccgcac gaagaacgct ccctgcgaat
     6121 cggtgaacac gaggtcgtcc ccgattcgaa gcgcggcccc tgagatcggc gacccgacgt
     6181 cgtcctgcac cgttcccttg accacgttgt ccgggatccc gaactcgggg accttgggcg
     6241 cggcgctcga gccgcgatag acgaactggc ttccctgcgc cgtgtagcgg acgcgcccgg
     6301 tcggatcgat gtagctcccc acctccgctt tgacgccgcc cggaccctgg agccgcaccg
     6361 tgagcatgag cgcctgctgg aacggtttct cctcgttgaa cggaacgtag accgtctggt
     6421 actcggcggc gac
//
LOCUS       DASTKC010000038        21783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1682748, whole genome shotgun sequence.
ACCESSION   DASTKC010000038 DASTKC010000000
VERSION     DASTKC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21783
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682748"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFR25_01465"
     CDS             complement(<1..378)
                     /locus_tag="VFR25_01465"
                     /inference="COORDINATES: protein motif:HMM:NF039894.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9939748.1"
                     /translation="MIGLSDPFTISTIDPAGLAARLGLAPGERILEINGVVLHDEIDF
                     GSQISEEELTLLVRAKDGVERTVRGLREYGVPFGAEFEARQPKRCHNNCVFCFVYQHP
                     KGVRRELLIKDDDYVFSFVHGNFI"
     gene            complement(505..579)
                     /locus_tag="VFR25_01470"
     tRNA            complement(505..579)
                     /locus_tag="VFR25_01470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(545..547),aa:Gly,seq:ccc)
     gene            complement(663..1586)
                     /gene="miaA"
                     /locus_tag="VFR25_01475"
     CDS             complement(663..1586)
                     /gene="miaA"
                     /locus_tag="VFR25_01475"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET9939749.1"
                     /translation="MTRTTPVVLLGATAGGKSEIALHLARLVGGEIVGADSRQIYRGL
                     EIGTAAPSASDRAVAPHHLASFLAPDEIYSAGRYAREAAPIIEDIAGRGRVPIVVGGS
                     GLYLRALLDGLFEGPERDPALRASLDERLRTEGLDALRADLQRVDPVAFASILPGDSV
                     RVIRALEVYALTGRPISELRRERRRKGVASRRFGIRWRREPLATRIANRIRDQLRDGF
                     LAQAQELMGAGYAPAAPGLLTLGYRELLAHLRGETSLDEAVATIALRTRQLAKRQETW
                     FRATGEVTWFDLESPEQFPEISRSIAATVRD"
     gene            complement(1597..3387)
                     /gene="mutL"
                     /locus_tag="VFR25_01480"
     CDS             complement(1597..3387)
                     /gene="mutL"
                     /locus_tag="VFR25_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9939750.1"
                     /translation="MPSVRLLDPSVIAKIRAGEVVDRPAAVAKELIENALDAGSRLIA
                     IQSASNPDRMIRVQDDGSGMGREDALLAVRQHATSKLERAEDLEAIATLGFRGEALAS
                     IAEVSRLTLSTRAADELTGTQIEILGGTVISVSGVGRAVGTTVSVEDLFYNTPARKRF
                     LRSREAEVRALARVVWSYALVTPHVHWRFRVEGREDTELPTAADLLERWQVFYGRGSA
                     ESAAPFEHEGAGYRFRGVLGAPEMARANREQQIFAVNGRVISSSSMAAALRQGFGNLI
                     PGDRHPVALLLVDIDPSQIDVNVHPTKREVRFRDESGLFQATRRAVENAMRVYVPVAL
                     GVPESGSMVPEEGSAGGTLVIEPGTVGEGAPSYVPEGIAPGRLFESAPATELGSPWAR
                     ERVVTADVKESLAEVEVPIWQLHDRYLLAPIRGGLVIVDQHAAHERVLYEEARIHLAD
                     EAGPSQHLLFPRVLDLSPPELEELLSMEPLVRRLGYEVSLFGERQVAIRGVPATLAEE
                     TAIDALKRLLAGEETHGEITRDDPPEERIAKSWACHAAVRSGQPLDPVERRALFDRLF
                     ATTLPHGDPHGRPTYVRVPLEELDRRFGRR"
     gene            complement(3390..4598)
                     /locus_tag="VFR25_01485"
     CDS             complement(3390..4598)
                     /locus_tag="VFR25_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9939751.1"
                     /translation="MTSTTAAPARDLGAQKLAQAQSLLAECGLDAWLTVVRESAERPD
                     PTLRYFLDLDFTWTTFFLVTPSSSAALVATFDAPDLRERGLFDKVVTYKEGPRAALLE
                     LLDETRPARIGINFSEHDALADGLTAGLRDSLESLLKGTPHQGRLVSAAPLLFRLRNV
                     KLPEERRRIERAVLETESMFDRVRDRIRLGMTARQIAEMFHEEAARAGVPTAWPSHHC
                     PTVTVGAKSPIGHVGPGDEPVTDGCIVHVDFGISRDGYCSDLQRLWYVKRDGDAPEPV
                     RRAYAAVAGAIDATARALIPGSIGWEIDQLARERIREGGYPEYAHALGHHLGRAVHDG
                     GGVLGPRWERYGEEPYEPVRLGAVYTLEPSVHLPDHGIVSLEEDVVVEEKGGVFLSRF
                     PRELPLLRTA"
     gene            complement(4649..6988)
                     /locus_tag="VFR25_01490"
     CDS             complement(4649..6988)
                     /locus_tag="VFR25_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9939752.1"
                     /translation="MRLAFLDLPDDRVDLISLASRTPSVEIVLVVHSDPEALSLEIAE
                     VLQIPRSAEPLDLLSLKPDQVALPSLEAPGAKALLRSGISERIFTSLDEIHSRLQDPV
                     PPTIQDIPIADGDADGDGVVAPTVQGRTARSSGDPEPLQAWEAMFDEATGGLGKIQDA
                     MDLTDDRSRVLNEILTLAVDQTGAEMGSIMILDEEEGELTIAFANGIPSETVRSTRQK
                     LGEGISGKVAQDRTPLLIHDPSPESRADPRERSRIRAAMCAPIQMGDRVLGVINVSSH
                     QADKRFEDLDLQRLVRIAERIATILDRVVQVQRRDADAAEFRARRGVEMALTREKLDQ
                     DERLRLAATRIASALRADAVQIYLAVPGEERFHVVSSGTAAGVQGRMPVGEGLVASVH
                     EDGEPLCLTARRSGSKGVLSRLILAPLQGRDSMGVIAIENRSLKEEETEEETRLVVRL
                     GRLIAQHIELHRGEGAIRQEQLLAKLADIAPRLMVPHDLDSLLLESAAALRAICPSGL
                     VSARLRGEGDEVAIRSSVAGSTADPTALTKLDEELGTLAVRDGKESASPEGSGSNTEV
                     RFACVPVRTADRVIGAVTAVVPRNETASSPLGAPELEAIRKLALYVALAWERTQSTEA
                     PAPSAIADPATGLIAASALEARVLEEVKRAERYHDKFLLTLCAIAGYHDLVERHGAEW
                     TEGLIRDFAKSLSRNVREVDLVARVGDGRFAVLSPETDKDHGAMLKRLDQLLLDAQKT
                     REVPYTEQVQLIGRQYVFPEDVPTGGEMVALLRGSYAVP"
     gene            complement(7203..9392)
                     /locus_tag="VFR25_01495"
     CDS             complement(7203..9392)
                     /locus_tag="VFR25_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939753.1"
                     /translation="MKATSDKERSPILNRASRLFGAIVIGAMLGHASPALAVKTSFWR
                     TDDLQGFLQGEHVAGVALESDGYLTLGPAWDSVATRLEGVSYIWCVARDSKGRVYFGT
                     GDNGRIYRWTREKGASVVWETGAGEITSLVIDKADNVYAGSAPGGIVYRVGAAGDTTR
                     YYETGEESIWSLLIGKDGTLYAGTGSKGKIYRITGAKKGTVLAETKDVNVLALAETED
                     GALLAGTASKGLLLRVDKNGGTRVIYDSDSDEVRGIAVLPDGSIVVGTNRQSKTGSDG
                     PGQGGAVGYAIEVTPSGSKCGVFLVQTDGSARLLYAPPTDFIYAVVPDGPKSVLVATG
                     ESAALFRVSTDKKYALIGVPEEKQILAIARSGNATYVATGNDAVLYELSSAPAKEGIY
                     TSQAYDLRSVASWGRMTAGLTGKGEVLWSTRSGLSEEPDEGWSAWSKDTPIKGSTPIE
                     SPPSRFLQYRLTFKGSGGDGPVVSWVEVAYQQRNLPPEIGDIKVYGPDNPYMEGGPDY
                     RPPQISQTFPSGLKIEYNMPRTGPKPVSDPSAAWARGVRTVSWEALDPNGDGLKYDVS
                     IKADDEKEWRPLVSDHPERVYSFDSESYPNGEYRVRVEAKDTPDNPPSVALHTERTGS
                     PFEIDNVPPRVEDLKTSKNAPKGGRESITVSGSAIDADTRITTIEYSVDGGDWTDIFP
                     EDGMFDEKQETFRFEVKDLSPGEHRISVRASDLERNVAVGKVLTVAR"
     gene            complement(9404..11095)
                     /locus_tag="VFR25_01500"
     CDS             complement(9404..11095)
                     /locus_tag="VFR25_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HET9939754.1"
                     /translation="MKAGMTGYGLTVFEGTRIDTFQITILGVLRGYRPGANLVLARAA
                     GHDLERTGIIAGMSGSPVYIHGKLLGAIAYAWAFNKDPVGGITPVEEMLSLFPPAGQP
                     TPDDVDQRLGLADFGTDGSPADAPAPGTNPLEDPTGVRAIAAPLTLSGFTPEAVSFLQ
                     PWLEKRGFVVSPGGGYQPGVVCDSLVPGSAVGVELIRGDWSAAAIGTLTYRDGDRLLA
                     FGHPFVAMGWVRFPLTAATIHTIFASQQISTKVGSPSTTCGTLVADRSIGVSGELGAP
                     PQMIPVHVAIQGTGKRDKRYNFEVVRNRLLTPSLVGSAVVNSISEALNDAGYATIDYD
                     LTFYMNAGARTVRKGNVFLTQSPIAGVGEQVSQSLQLLMSDHFRPSTLDSARIDVRAT
                     LGLEAARISDVRVRPASAAPGDSVEVEITLRRGGAAKETRRTRLYIPPQTPEGELSIR
                     VADGDETDRWERERTPDLYQPDTFDQLVQVIESERRLDRIYVQLFRQAGGATVRGGEI
                     SQPPASVLGVLTADRKSGAVSATKGATLSEKEIPMDVVVRGSETAKLDVVPDLSR"
     gene            complement(11218..13872)
                     /gene="mutS"
                     /locus_tag="VFR25_01505"
     CDS             complement(11218..13872)
                     /gene="mutS"
                     /locus_tag="VFR25_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9939755.1"
                     /translation="MESTPMLDQYRRLKAEHPGCILLFRMGDFYETFGDDALIASRVL
                     GIALTSRDKKRDPLPLAGVPHHAVESYLKKLVQHGYSVAIAEQMEAASEAKGLVERQV
                     IEVVTPGTVTRPGLLDTQESNFIVALLPGEERVGVAVAEVSTGEFRVGHVAPTDLEAI
                     AIEFPAREVLVPEGAFRSGDATGANGLASLGFSGATPTRWEPARFDARSGEAELKRRF
                     QVLSLEAFGLDGAREGMGAAGALLAYLQTLKKSDLPQFRDLRPLREGAPLVVDEVTLR
                     NLEVLASPAGPDHTLLALLDRTETSMGARALRALLRAPTSDRALLDARLARTECFAAS
                     AVLRGEMRDRLHRVPDLERCLGLLGSGRATPRDLGSLRDGLRRLPGLRLALEGRSHPV
                     LDAWRRALPDLSNLAAELEAALVEELPLHATQGGIVRPGYDDELDRLRGDASDIRARV
                     LALEARERERTGIVNLRVLYHRTFGYLIEVTRSQLGRVPDDYIRRQTLTGAERFATPE
                     LSQMEQRIEASSIESHKIEAAHFQRIRDLALGRIGDLTAAARVIAEIDLHLSLGDVAG
                     RERWVKPALTDDRRLILERARHPMVERALPPGGFVPNDCVLDPDVEQVWLITGPNMGG
                     KSTFLRQVGLCVYLAQIGSFVPCAAATVGLVDRIFTRVGASDQIARGASTFFIEMKET
                     ATILRQATDRSLVLLDEVGRGTSTYDGLSLAWAVTEALHDGPEARPRTIFATHYHELT
                     ELTDHLPRLRNRTVRVAEEGGEIVFLHQIAPGRADRSYGIHVAQLAGVTEPVLRRARD
                     VLARLEAEHARPVPERTAARKRGTPVEQGDGGVSSEERRALLTALADLPVESLTPVQA
                     LNELARLREQARSSAGAAALDPLQHSRE"
     gene            complement(13881..14993)
                     /locus_tag="VFR25_01510"
     CDS             complement(13881..14993)
                     /locus_tag="VFR25_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET9939756.1"
                     /translation="MSGVLRMGARVSLPVRSLALRGILAALVLALAAASLGAKTASKP
                     APNRGPSPEERAIALAREALADGNVEGARGYLAPVTSGRVPHEKEDDFAYLTAVLIED
                     GDAYQEALQNYLRDYPKGLHRREATLALAKVRYVRGEYSESERLLATFSPGVEKDFVG
                     RQALVLLGLAQLARGDAVGALAGLHQAAPDLEGSPQEEAYDFALAQSALHASRPADAG
                     DALKRLLEGHPKGDYVPQALYALGTGLEAVGRTGDASGVFRQVMVRYPASYEATRVRD
                     RGIRITVEPEAPGPLALRGGFALQVGAFSRRDLAESLAKDLKTSGVEDVSVKAGPETP
                     PVFRVRAGAFTTRDEAKAMGERLRRERGFSYTIVPR"
     gene            complement(14960..16093)
                     /locus_tag="VFR25_01515"
     CDS             complement(14960..16093)
                     /locus_tag="VFR25_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9939757.1"
                     /translation="MSEFATVLVVGGLLTGIGYAAWVLSRDAGASPPPPETPYQHALN
                     ALLAGDRAEALSAFAETVRQDSDNIDAYIHLANLLRERGDAARALQIRRDLTVRAGLS
                     AGQERAIREGLVRDLIALGRPADAVAEARELRDLDKKNGSALKLLMKAHEAAGEWERA
                     YETRAEVAKATSENGVGLARYRSSIGEIYLREGKLDDAKRQFQAALKLKQDEPAALLR
                     LGDLCYETNRPERAVVLWKALAEAHPTFSHLVLDRLETSYFESGRFADVDQTYEEMLA
                     RNPKDVRVHLALARVHLKKGDLDDAGRMLNEAFELEPGSVPARLLLAELHRRRGDLPR
                     ALDEMQSLIERFGPGEVYICPSCGHESEEYWSRCPECFAWARA"
     gene            complement(16090..16419)
                     /locus_tag="VFR25_01520"
     CDS             complement(16090..16419)
                     /locus_tag="VFR25_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET9939758.1"
                     /translation="MWLLKNLIWLIIMILVVGFAILNVNETVTSVILPGRIYRTLPSN
                     VALFSAFVIGMLTAFLLTLAHQLKTRAGVARVRRENQDLKRELDGLRNLPLEDLTLKE
                     TAGAARE"
     gene            complement(16495..17892)
                     /gene="mtaB"
                     /locus_tag="VFR25_01525"
     CDS             complement(16495..17892)
                     /gene="mtaB"
                     /locus_tag="VFR25_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HET9939759.1"
                     /translation="MSSAPRVSFLTLGCRLNQHDTAAMRARLADAGWTQVDAPEGADV
                     VVVNTCTVTARADQEARQLVRSLARRAPSARIVVTGCYAQRAPGEVAAIPGVSLVLGT
                     AERERIELYVGAGSAAWKGAAAGALRRASRDAREGRSAFPGHASYLDEQLDAIPLHVG
                     PGRAKRAFVASSPVAFGRTRALLKVQDGCDAFCSYCVVPYVRGRARSLPLDEAVAQGA
                     RLLEAGFHEIVVTGADLGSYGSDLGKPGLLAPLVERLLALGSAHRVRISSIEPEKVPA
                     GLVEMIGSEERLCPHLHLPLQSGSDAILAAMRRRYRVRDYARLLERVTARGTVAIGAD
                     VIVGYPGEGDAEFEETGRFIDGAPLSYLHVFRYSARPGTSAAPLPAPMTARERAAELR
                     ALGLAKERAFYESLVGTTRSVLLEGRSDAASVRGRADVYAPVLVRSTPRWVGLARVVL
                     REAGQDGCVGELATR"
     gene            complement(17933..19762)
                     /locus_tag="VFR25_01530"
     CDS             complement(17933..19762)
                     /locus_tag="VFR25_01530"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9939760.1"
                     /translation="MDLKDDAADDAEVEAKPRKRKAITRLVHEDLGEEAGGVAVGVGE
                     LDPDEDAQSSMGQWLQLYEDSLKDLEEGEIVRGRVLKIDDKEVTVDVGFKSEGVIPVE
                     EFPDLEGVKVGDEIEVFLEKTENQDGLVVLSKQRADFVKVWDRVKTASDNGELVEGKL
                     VRKIKGGVVVDLYGVEAFLPGSQIALRQVQNVDSLIGQSMEFKIIKLNKRRRNIVVSR
                     RAVLEEHRAKQKSEIIQELAKDQIREGVVKNITDFGAFVDLGGIDGLLHITDMSWGRV
                     SHPSELVSIGDKVRVKVLSFDPEKERISLGMKQLTPYPWEDVDKRYTVTQRVKGKVVS
                     ITDYGAFVELEKGIEGLIHISEMSWTRHVRHPSKVVAIGDQIEAMILKIDKDNEKISL
                     GLKQIEPDPWLTLDDRFPIGTRLTGKVRNLTNFGAFVEIEEGIDGLVHISDMSWTRRV
                     VHPSEVLKKGDKVEVVVLSIDKDARRISLGLKQVSDDPWPTLAERYLPDMIVRGKVVR
                     LLDRGVIVDLEDGLEGFIPLSQLGIEGLKKPSDSFKPEDTLDLKVTRVDTQAHRIILS
                     VKAWLTDQDAATQAAFAEKYKPTPSADNEPVATEETSDSGAAS"
     gene            complement(19848..20708)
                     /gene="ispH"
                     /locus_tag="VFR25_01535"
     CDS             complement(19848..20708)
                     /gene="ispH"
                     /locus_tag="VFR25_01535"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET9939761.1"
                     /translation="MEVIVADNAGFCFGVKRAIKMANEAMDQDPASVKALGPLIHNPQ
                     VVASFRNRGLEVVSELEQVDQGSTVIIRSHGVGPEVKASAETRGLKVVDTTCPFVTKA
                     QQYAAKLIDENYKVVMIGDQHHPEVIGVIAHTGGKAIVIDTVAEAEALKFIPRMGVVF
                     QTTHAIGHVQEIVGALLKRGKEVRVFNTLCGATTSMQKTAVELAPSVEAMVIIGGRQS
                     ANTAQLAEVCRKVNPNVYQIESAEEIVPDWFQGMRQIGVSAGASTPDEVIAEVVDRLT
                     QIEPAQAAGA"
     gene            complement(20710..>21783)
                     /gene="cmk"
                     /locus_tag="VFR25_01540"
     CDS             complement(20710..>21783)
                     /gene="cmk"
                     /locus_tag="VFR25_01540"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9939762.1"
                     /translation="EAGPQGLTIGGELVEPHIRTAEAGDAASQVAVHPKLRKHLVKIQ
                     RSLAKAPGLVMEGRDIGTVVFPKADLKIFLTASAEARTERRVAQLRAAGLPADPAEIE
                     AAIRERDRRDSAREASPLAPAPDAFPLDTTSLTLEQQVDLAVRWAELARRGPGKRTLF
                     YLFGHHASGIFCRTFLRLRITGMEHIPAKGPVIIACNHISFWDPPIMGGVVPRRLLFL
                     AKEELFQNRWFGALIRGYNSIPIQRGPGARSALRGAERVLDEGGAVLIFPEGTRSKTG
                     SFLPPRAGIGRLAATARAPVLPARISGSRQIRRSMARQVEIRLAFGSMIMPPVGQVES
                     RADADAFARRVMDAIGDLPGGAY"
BASE COUNT         3355 a         7568 c         7406 g         3454 t
ORIGIN      
        1 gatgaagttt ccgtggacga acgagaagac gtagtcgtcg tccttgatga ggagctcgcg
       61 ccgcacgccc ttcgggtgct ggtagacgaa gcagaagacg cagttgttgt ggcagcgctt
      121 gggctgccgc gcctcgaact ccgcgccgaa ggggacgccg tactccctaa gcccgcgcac
      181 ggtccgctcc acgccgtcct tcgcccggac gaggagggtc agctcctcct cggaaatctg
      241 ggagccgaag tcgatctcgt cgtggagaac gaccccgttg atctcgagga tccgctcgcc
      301 cggcgcgagg ccgaggcggg cggccaggcc ggccgggtcg atcgtggaga tggtgaaggg
      361 atcgctcagg ccaatcacgg ccgcggttcc tcgctcaggg ctatggagtc aagaggcatg
      421 cgtcgaactc gtgaattatg aggcacttcg ctctctgtca tcaaggagtg ccgcacgttc
      481 ggcggccgtc cgcggaggga gaactggagc gggcaacggg atttgaaccc gcgaccttca
      541 gcttgggaag ctgacactct accactgagt tatgcccgct ccctcaacga aggcagccta
      601 ggtgcttccg ccgcaagggt caagcccaat cgggacatac ggacagacgt ggtcgcggcc
      661 ggtcagtctc gaacggtcgc cgcgatcgat cgcgagatct cggggaattg ctccggcgac
      721 tcgaggtcga accacgtcac ctcccccgtc gcccggaacc aggtctcctg gcgcttggcg
      781 agctgccggg ttcggagcgc gatcgtcgcg accgcctcgt ccagcgaggt ctccccgcgg
      841 agatgtgcga ggagctcccg gtagccgagc gtgaggagcc ccggcgcggc gggcgcgtag
      901 cccgccccca tgagctcctg ggcctgcgcg aggaatccat cgcgaagctg gtcccggatc
      961 cggttcgcga tccgcgtcgc gagaggctcg cgccgccagc ggatgccgaa tcggcgcgag
     1021 gcgactccct tcctccggcg ctcgcggcga agctccgaga tgggacgccc cgtgagcgcg
     1081 tacacctcga gcgctcggat gacgcgcacg gaatcccctg ggaggatcga ggcgaaggcc
     1141 accgggtcca ccctttggag gtcggcccga agcgcatcga gcccttccgt gcgaagccgc
     1201 tcatcgagcg aggcgcgaag cgcgggatcg cgctcgggac cctcgaagag accgtcgagg
     1261 agcgcgcgca gatagaggcc tgagcccccg accacgatcg gcacgcgacc gcgccccgca
     1321 atgtcctcga tgatgggagc cgcctcgcgc gcgtagcggc cggcgctgta gatctcgtcg
     1381 ggagcgagga aggaggccag gtggtgcggc gcaacggcgc gatcgctcgc gctcggcgcc
     1441 gcggtcccga tctcgagacc gcgatagatc tgccgggaat ccgctccgac gatctcgccg
     1501 ccgacgagcc gggccaggtg gagcgcgatc tcgctcttcc ctcccgcggt cgcgccgaga
     1561 aggacaacgg gggtcgtgcg cgtcacggcg aagaggtcag cgccgcccga agcggcggtc
     1621 cagctcctcc aggggaacgc ggacgtaggt gggacgtccg tgcggatcgc cgtgcgggag
     1681 cgtggtcgca aacaggcggt cgaacagcgc tcgacgctcg accggatcga gcggctgccc
     1741 cgagcggacc gcggcatggc aggcccagct cttggcgatg cgctcctcgg gaggatcgtc
     1801 gcgcgtgatc tcgccgtgcg tctcctctcc tgcgaggagg cgcttcagag cgtcgatcgc
     1861 ggtctcctcg gcgagagtcg cgggtacgcc gcgaatcgcg acctgccgct cgccgaagag
     1921 cgagacttcg tagcccaggc gtcgcacgag cggctccatc gacaggagct cttccagctc
     1981 cggcgggctc agatcgagaa cgcgcgggaa gaggaggtgc tggctcggcc ccgcctcgtc
     2041 ggcgagatgg atccgcgctt cctcgtagag gacgcgctcg tgcgccgcgt gctgatccac
     2101 gatgacgagg ccgccgcgaa tcggcgcgag gaggtagcgg tcgtggagct gccagatcgg
     2161 aacctcgacc tcggccaggc tctccttgac gtccgccgtc accacgcgct cgcgggccca
     2221 gggcgatccc agctccgtcg cgggggccga ttcgaagaga cgccccggcg cgattccctc
     2281 gggcacgtac gaaggcgctc cctcccccac ggtcccgggc tcgatcacga gcgtcccgcc
     2341 cgcgcttccc tcctccggca ccatcgaccc cgactcgggc acgccgagcg ccacgggcac
     2401 gtacacgcgc atcgcgttct cgaccgcgcg ccgcgtcgcc tggaagagtc ccgactcgtc
     2461 gcggaagcga acctcccgct tcgtgggatg cacgttcacg tcgatctgcg aggggtcgat
     2521 gtcgacgagg aggagcgcca ccggatggcg atcgccggga atcaggtttc cgaatccctg
     2581 gcgaagggcc gcggccatgg acgaggacga gatcacgcgc ccgttcaccg cgaagatctg
     2641 ctgctcgcgg ttcgcgcgcg ccatctcggg ggcgcccagc acgccgcgga agcggtatcc
     2701 ggcgccttcg tgctcgaacg gcgccgcgct ctcggcggag ccgcggccgt agaagacctg
     2761 ccagcgctcg aggagatcgg cggcggtcgg aagctccgtg tcttcgcgcc cctcgacgcg
     2821 gaagcgccag tggacgtgcg gcgtcacgag ggcgtagctc cagacgacgc gcgcgagcgc
     2881 gcgcacctcg gcctcgcgcg atcgcaggaa tcgcttccgc gcgggcgtgt tgtagaagag
     2941 atcctcgacc gagaccgtcg ttccgaccgc gcggcccacg ccggagaccg agatcaccgt
     3001 gccgcccagg atctcgatct gcgtgcccgt cagctcgtcg gcggcgcggg tggagagcgt
     3061 gagcctcgag acctccgcga tcgaggcgag cgcctcgccg cggaatccga gcgtggcgat
     3121 ggcctcgaga tcctcggcgc gctcgagctt gctcgtcgca tgctggcgga ccgcgagaag
     3181 cgcgtcctcg cgccccatgc ccgacccgtc gtcctgcacg cggatcatgc gatcggggtt
     3241 ggacgccgac tggatggcga tgagccggct ccccgcgtcg agagcgttct cgatcagctc
     3301 cttggcgacg gcggccgggc gatcgacgac ttccccggcg cggatcttcg cgatgacgga
     3361 gggatcgagg aggcgaaccg agggcatcct taggccgttc gcaagagcgg caactcgcgc
     3421 ggaaaccggg agaggaacac gccgcctttc tcctcgacga cgacgtcctc ttccagcgac
     3481 acgatgccgt ggtcggggag atgcacgctc ggctcgagcg tgtagaccgc ccccagacgg
     3541 accggctcgt agggctcctc gccgtagcgc tcccagcggg gtcccagcac gcctccgccg
     3601 tcgtgcaccg cgcggcccag atgatgcccc agtgcgtgcg cgtactcggg gtagcctccc
     3661 tcgcggattc gctcccgcgc gagctgatcg atctcccatc cgatggaccc aggaatgagc
     3721 gcgcgagcgg tggcgtcgat ggctcccgcg accgccgcgt acgcgcgtcg cacaggctcc
     3781 ggcgcgtccc cgtcccgctt cacgtaccag aggcgctgga ggtcggaaca gtagccgtcg
     3841 cgcgagatgc cgaaatcgac gtggacgatg cagccatccg tcaccggctc gtccccgggg
     3901 ccgacgtggc cgatcggcga cttcgcgccg acggtgaccg tggggcaatg gtgggacggc
     3961 catgcggtcg gaacgccggc acgcgcggct tcctcgtgga acatctccgc gatctgtctc
     4021 gcggtcattc cgagtcggat ccggtcccgc acgcgatcga acatgctctc ggtctcgaga
     4081 acggcgcgct cgatccggcg gcgctcctcc gggagcttca cgtttcgtag cctgaagagg
     4141 agtggcgcgg cgctcacgag gcggccctga tgcggcgtgc ctttgaggag cgactcgagc
     4201 gaatcgcgga gacccgcggt caagccgtcc gcgagggcat cgtgctcgga gaagttgatc
     4261 ccgatccgcg cgggccgggt ctcgtccagg agctcgagga gcgcggcgcg cggcccttcc
     4321 ttgtaggtca caaccttgtc gaagagtccc cgctccctga gatcgggggc gtcgaaggtc
     4381 gcgacgagcg ccgcggacga gctcggggtg acgaggaaga aggtggtcca ggtgaagtcg
     4441 aggtcgagaa agtaccggag cgtcggatcg ggccgctccg ccgattcccg gaccacggtg
     4501 agccaggcgt cgagcccgca ttcggcgagg agggactgcg cctgtgcgag cttctgtgcc
     4561 ccgagatcgc gcgccggtgc cgccgtggtc gatgtcatgc gccaaccata gcccgccgcg
     4621 cttcgcgcgg caagggcgga ccgcgcgatc agggcaccgc gtagcttccg cggaggagcg
     4681 cgaccatctc tcccccggtc gggacatcct ccgggaatac gtactgccgt ccgatgagct
     4741 gcacctgctc ggtgtacggg acctcgcggg tcttctgcgc gtcgaggagg agctgatcga
     4801 ggcgcttcag catcgcgcca tggtccttgt cggtctccgg cgagagcacg gcgaaccgac
     4861 cgtcgccgac ccgcgcgacc aggtccacct cacgcacatt ccgggacagc gacttcgcga
     4921 agtcccggat caggccctcg gtccactcgg ctccatgccg ctcgaccagg tcgtgatagc
     4981 ccgcgatggc gcacagggtg aggaggaact tgtcatggta gcgctccgcg cgcttcacct
     5041 cttcgaggac gcgggcttcc agcgcggagg ccgcgatcag cccggtggcg gggtccgcga
     5101 tcgcggatgg cgcgggggcc tccgtgctct gcgtgcgctc ccacgcgaga gcgacgtaca
     5161 acgcgagctt tcggatcgcc tcgagctccg gggcgccgag cggggacgac gcggtctcat
     5221 tgcgaggaac gaccgctgtc accgcgccga tcacgcggtc cgccgtgcgc accgggacgc
     5281 aggcgaaccg aacttccgtg ttcgaaccac tcccctcagg cgatgcagac tccttgccgt
     5341 cccgcaccgc aagcgtgccg agctcttcgt cgagcttcgt gagcgccgtg ggatccgccg
     5401 tcgatcctgc aaccgatgac cggatcgcga cctcgtcgcc ctcgccgcgc agacgagccg
     5461 agacgagccc actcgggcag atcgcgcgaa gagcggccgc gctctcgagg agcagcgaat
     5521 cgaggtcgtg cggcaccatg aggcggggag cgatgtccgc gagcttcgcg aggagctgct
     5581 cctggcggat ggccccttcg ccccggtgca gctcgatgtg ctgcgcgatg agacggccca
     5641 gccggaccac gaggcgggtc tcctcctcgg tctcctcttc cttgagcgac cgattctcga
     5701 tcgcgatcac tcccatggag tctcttccct ggagcggcgc gaggatgagt cgcgagagga
     5761 cgcccttgga gccggagcgc cgcgcggtca ggcacagggg ctccccgtcc tcgtgaacgc
     5821 tcgcgacgag tccttcgccg acgggcatgc gtccctggac cccggccgcc gtccccgagg
     5881 acacgacatg gaatcgttcc tcccccggca cggcgagata gatctggacc gcatcggccc
     5941 ggagcgcgct cgcgatgcgc gtggccgcga ggcggagccg ctcgtcctga tccagcttct
     6001 cgcgcgtgag cgccatctcc acgccgcgcc gcgcgcggaa ctcagcggca tccgcatcac
     6061 gccgctggac ctgcaccaca cggtcgagaa tcgttgcgat gcgctccgcg atccgaacga
     6121 ggcgttggag gtccagatcc tcgaatcgct tgtcggcctg atggctcgac acgttgatga
     6181 ccccgagcac tcgatccccc atctggatcg gggcgcacat ggccgcacgg atgcgcgagc
     6241 gctcgcgggg gtccgcgcgc gattccggcg acgggtcgtg gatcaggagt ggcgtccgat
     6301 cctgggccac cttgcccgag atcccctcgc ccagcttctg gcgcgtggag cgcacggtct
     6361 ccgagggaat gccgttcgcg aacgcgatcg tgagctctcc ctcctcctcg tcgaggatca
     6421 tgatcgatcc catctccgcg ccggtctgat ccaccgcgag ggtcaggatc tcgttcagca
     6481 cgcggcttcg atcatcggtc agatccatcg cgtcctggat cttgccgagg cctccggtgg
     6541 cctcgtcgaa catggcttcc cacgcctgga ggggctccgg atcgccgctc gagcgcgcgg
     6601 tgcgcccctg aacggtcggc gcgacgacgc catcgccgtc cgcgtccccg tcggcgatgg
     6661 gaatgtcctg gatcgtgggt ggaacggggt cttgaaggcg ggagtggatc tcgtcgagcg
     6721 aggtgaagat tcgctcggag atccccgagc ggaggagcgc cttggccccc ggcgcctcca
     6781 gcgacggaag cgcgacctgg tcgggcttca gggagagaag atcgagcggc tcggcggagc
     6841 gaggaatctg caggacttca gcgatctcga gcgagagcgc ttccggatcg gaatgaacga
     6901 cgagcacgat ctccaccgac ggcgtcctgc tcgcaagcga gatcaggtcg acgcggtcat
     6961 cgggcaggtc gagaaaggcc agcctcatgc cacccctgag gtttgcagcg gatgtgagaa
     7021 gggataatga gccgaagcgc aaaccgcaag gattgtacca ccggacgggg gttgcggcga
     7081 cggaatgagg cgcttccggc acaacgggtt agcgccccgc ggcaccgcct ggacgcctca
     7141 aacgacgcgg gccgctcggc tcccgatccg ggaaccggcg gcccgcgttg cgttctgcac
     7201 gatcagcggg cgacggtgag caccttgccg accgcgacgt tgcgctcgag atcggacgcg
     7261 cgcaccgaga tccggtgctc gcccggtgag agatccttca cctcgaaccg gaaggtctcc
     7321 tgcttctcgt cgaacatgcc gtcctcggga aagatgtccg tccagtcccc tccgtccacc
     7381 gagtactcga tcgtcgtgat ccgcgtgtcg gcgtcgatgg ccgatccgct caccgtgatc
     7441 gactcgcgcc cgcccttcgg cgcgttcttc gacgtcttca ggtcctcgac gcgcggcgga
     7501 acgttgtcga tctcgaacgg gctgcctgtg cgctcggtgt ggagtgccac cgacggcgga
     7561 ttgtccggcg tgtccttcgc ctccacgcgc acgcgatact cgccgttcgg ataggactcg
     7621 gagtcgaagc tgtagacacg ctcgggatgg tcggacacga gcggccgcca ctccttctcg
     7681 tcgtcggcct tgatcgagac gtcgtacttg aggccgtcgc cattcggatc gagcgcttcc
     7741 cacgacaccg ttcgaacgcc gcgcgcccat gcggcgctgg gatcggacac cggcttcggg
     7801 ccggtgcgcg gcatgttgta ctcgatcttc agaccgctcg ggaaggtctg tgagatctgc
     7861 ggcgggcgat agtccggacc gccttccatg tatggattgt cgggaccgta gaccttgatg
     7921 tcgccgatct cgggcgggag gttgcgctgc tgatacgcga cctcgaccca cgagacgacc
     7981 ggaccgtcgc ccccgctccc cttgaacgtc aggcgatact ggaggaatcg cgacggcggg
     8041 ctctcgatgg gcgtgctccc cttgatcggc gtgtccttgg accaggcgct ccatccttcg
     8101 tccggctcct cggagaggcc gctgcgcgtg gaccagagaa cctcgccctt ccccgtgagt
     8161 ccggcggtca tgcgtcccca ggacgcgacg gagcgaagat cgtaggcctg cgaggtgtag
     8221 atgccttcct tcgcgggcgc cgagctcaat tcgtaaagca cggcgtcgtt cccggtcgcg
     8281 acgtaggtcg cattgccgga gcgcgcgatc gcgaggatct gcttctcctc agggacgccg
     8341 atcagcgcgt acttcttgtc ggtgctcacg cggaagagcg cggcggactc gccggtcgcc
     8401 acgagcacgc tcttcgggcc gtcgggaacg accgcataga tgaaatcggt cggcggcgcg
     8461 tagagaaggc gggcgcttcc atcggtctgg acgaggaaga cgccgcactt gctgccgctc
     8521 ggcgtgacct cgatcgcgta gcccaccgcg cccccctgtc cgggcccgtc ggagcccgtc
     8581 ttggactggc ggttggttcc cacgacgatc gagccgtccg ggagaacggc gatgccgcgg
     8641 acctcatccg agtccgaatc gtagatgacg cgcgttccgc cgttcttgtc gacgcgaagc
     8701 agaagtccct tcgacgccgt gcccgcgagg agcgcgccgt cctccgtctc ggcgagcgcg
     8761 aggacgttca cgtccttcgt ctcggcgagg acggtaccct tcttcgcccc ggtgatccgg
     8821 tagatctttc ccttcgagcc cgtgccggcg tagagcgtgc cgtccttccc gatgaggagg
     8881 gaccagatgg actcctcgcc cgtctcgtag tagcgggtcg tgtcccccgc cgcgccgacc
     8941 cggtacacga tgccgcccgg agcggagccg gcgtacacgt tgtccgcctt gtcgatcacg
     9001 agcgaggtga tctcgcccgc gccggtctcc cagacgacgc tcgcgccctt ctcgcgggtc
     9061 cagcgataga tccgcccgtt gtcacccgtg ccgaagtaga cgcgcccctt ggaatcgcgc
     9121 gccacgcacc agatgtagga gaccccttcg agccgcgtgg cgaccgagtc ccacgcgggg
     9181 ccgagcgtca gatagccgtc gctctccaga gcgacccccg cgacgtgctc tccctggagg
     9241 aatccctgca gatcgtccgt cctccagaac gacgtcttca cggccagggc tggggacgca
     9301 tgccccagca tcgcgccgat cacgatcgcg ccgaacagcc tagaggctcg gttcagaatc
     9361 ggactccttt ccttgtcgga cgtggccttc atggatgacg ggttcatcgg gagaggtccg
     9421 gaacgacgtc gagcttcgcg gtctcgctgc cccgaaccac gacatccatc ggaatttcct
     9481 tctcggagag cgtcgctccc ttggtggccg agacggcgcc tgatttcctg tccgcggtga
     9541 gcacgccgag gaccgaagcg ggcggctgcg agatctcgcc cccgcgcacg gtcgcgccgc
     9601 ctgcctgccg gaagagctgc acgtagatcc ggtcgaggcg ccgctctgat tcgatgacct
     9661 ggacgagctg gtcgaacgtg tcgggctggt acagatcggg cgtgcgctcg cgctcccacc
     9721 ggtcggtttc atcgccgtcg gcgacgcgaa tgctcagctc gccttcgggc gtctgcggtg
     9781 gaatgtagag gcgcgtgcgc cgcgtctcct tcgccgcgcc cccgcggcgg agcgtgatct
     9841 cgacctcgac ggaatcgccg ggagcggccg aagcgggacg cacgcgcacg tccgagatgc
     9901 gcgccgcttc gagacccaag gtcgcccgca cgtcgatcct cgcggagtcg agcgtggagg
     9961 gacggaagtg gtcgctcatc aagagctgga gcgattgact cacctgctcc ccaacccccg
    10021 cgatcgggga ctgcgtgagg aacacgttcc ccttccgcac cgtccgggcg ccggcgttca
    10081 tgtagaacgt gaggtcgtaa tcgatcgtcg cgtagcccgc gtcgttgagc gcctcggaga
    10141 tcgagttgac gaccgccgat ccgacgaggc tcggggtcag gaggcgattg cgcacgacct
    10201 cgaagttgta gcgcttgtca cgctttccgg tgccctggat ggcgacgtga accgggatca
    10261 tctgaggggg cgcgcccagc tcgcccgaga cgccgatcga gcgatcggcg acgagcgtgc
    10321 cgcaggtcgt gctcggcgag cccaccttgg tggagatctg ctgacttgcg aagatcgtgt
    10381 ggatcgtggc cgccgtgagc gggaatcgca cccaccccat cgcgacgaac ggatggccga
    10441 acgcgaggag ccggtcgccg tcgcggtagg tcagcgtgcc gatggcggcc gcggaccagt
    10501 cgccgcggat cagctccaca ccgaccgcgg atccggggac gagagagtcg cacaccacgc
    10561 ccggctggta gccgccgccc gggctcacga cgaagccgcg cttctcgagc cagggttgga
    10621 ggaagctgac cgcctcgggg gtgaagccgg agagcgtgag cggcgccgcg atggcgcgca
    10681 caccggtcgg atcctcgagg ggattcgtgc cgggtgcggg agcgtccgcg ggcgatccgt
    10741 ccgtcccgaa atcggcgagg ccgagccttt gatccacgtc gtccggtgtc ggctggccgg
    10801 ccggcgggaa gaggctcagc atttcctcga cgggcgtgat cccgccgacg ggatccttgt
    10861 tgaaggccca ggcgtaagcg atcgctccga ggagcttccc atggatgtag accgggctcc
    10921 cgctcatgcc ggcgatgatc ccggtccgct cgaggtcgtg accggcggcg cgagccagga
    10981 cgaggttggc tcccggccgg tagccccgga ggacgcccag gatcgtgatc tggaacgtgt
    11041 cgatccgggt cccctcgaag accgtgagtc cgtatcccgt catgccggcc ttcacggagt
    11101 ccaccgggaa gacgcccggc aagggacggg ccgaagctgc gacggaacgg tgggcggctg
    11161 gaacgatcag agaagctgca agcagcgtca ggacggcggc tttacgaggt aaattcatca
    11221 ttcccttgaa tgttggagtg gatccagtgc ggccgcgccg gccgagctgc gggcctgctc
    11281 gcggagccgg gccagctcgt tcaaggcctg gaccggcgtg agggattcca ccggaagatc
    11341 cgccagcgcc gtgagaagcg cccgccgttc ctccgaggag acccccccgt ctccctgctc
    11401 tacgggtgta ccccgtttcc gcgcggccgt tcgttccggg accggacggg cgtgctccgc
    11461 ctccagacgg gccaggacgt cccgcgcgcg acggagcacc ggctcggtca cgcccgcgag
    11521 ctgggcgacg tggataccgt acgatcgatc ggctcttccg ggggcgatct ggtgcaggaa
    11581 aacgatctcc ccaccctcct ccgccacccg gaccgtacgg ttccgtagcc gaggcaggtg
    11641 gtccgtcagc tccgtcaact catgatagtg cgtcgcgaag atggtgcggg gccgcgcttc
    11701 gggaccgtcg tggagcgctt ccgtcaccgc ccaggcgaga ctcaagccgt cgtacgtgct
    11761 cgtcccccgg cccacctcgt ccaggagcac caggctccgg tcggtcgcct ggcggaggat
    11821 cgtcgcggtc tccttcatct cgatgaagaa ggtcgaggct ccacgggcga tctgatccga
    11881 cgcccccacg cgggtgaaga ttcgatcgac gagccccacc gtcgcggccg cgcacgggac
    11941 gaacgatccg atctgcgcga gatagacgca gagccccacc tggcggagga acgtcgattt
    12001 tcctcccatg ttcggaccgg tgatgagcca gacctgctct acgtccggat cgagaacgca
    12061 gtcgttcggc acgaatcccc cgggcgggag cgcacgctcc accatgggat ggcgcgcgcg
    12121 ctcgaggatc aaccggcgat cgtccgtgag cgcgggcttg acccagcgct cgcgtcccgc
    12181 cacgtcgccg agcgagagat ggagatcgat ctccgcgatg acgcgcgctg ccgcggtcag
    12241 gtcgccgatc cgtccgagcg cgaggtcgcg gatcctctgg aaatgcgccg cctcgatctt
    12301 gtggctctcg atgctcgacg cctcgatccg ctgctccatc tggctcagct ccggcgtggc
    12361 gaagcgctcc gcgccggtca gggtctggcg acgaatgtaa tcgtccggca cgcggccaag
    12421 ctgggagcgg gtgacctcga tgagatagcc gaacgtccgg tgatagagga cgcgcaggtt
    12481 cacgatcccc gtacgctccc gctcgcgcgc ttcgagcgcg agcacgcgcg cgcggatgtc
    12541 ggaggcgtcg ccgcgcaggc gatcgagctc gtcatcgtag ccggggcgca cgatgccccc
    12601 ctgcgtggcg tggaggggga gctcctcgac gagcgccgcc tcgagctcgg ccgcgagatt
    12661 cgagaggtcc ggcagcgcgc ggcgccaagc gtccagcacc ggatgcgagc gcccttcgag
    12721 cgcaagcctc aaaccaggca ggcgccggag cccatcgcga agcgatccga ggtcgcgcgg
    12781 ggttgcgcgc cccgagccga gaaggccgag acagcgttcg aggtcgggga ccctgtgaag
    12841 ccggtcccgc atctcgcctc gaaggacggc cgaagcggcg aagcactccg tcctggccag
    12901 cctcgcgtcg agaagcgcgc gatcgctcgt cggagcgcga agcaaggcgc gaagggcgcg
    12961 cgcccccatc gacgtctccg tgcggtcgag gagcgcgagc agtgtgtgat ccggcccggc
    13021 gggcgacgcg aggacttcga gattccggag cgtgacctcg tccacgacga gcggcgcgcc
    13081 ctcgcggagc ggccggaggt cgcggaactg cggcaggtcg gacttcttca gcgtctggag
    13141 ataggcgaga agcgcccctg cggcgcccat cccctcgcgc gctccgtcca ggccgaaggc
    13201 ctcgaggctc agcacctgga agcgccgctt cagctcggcc tctcccgacc gggcatcgaa
    13261 gcgagccggc tcccagcggg tcggcgtcgc cccgctgaag cccagggagg cgaggccgtt
    13321 cgcgcccgtg gcgtcacccg atcggaacgc gccctccggg acgaggacct cgcgcgccgg
    13381 gaactcgatc gcgatcgctt cgagatcggt cggcgccacg tggcccacgc ggaactcgcc
    13441 tgtcgacact tcggccaccg cgacgccgac gcgctcctca cccggcagga gcgccacgat
    13501 gaagttgctc tcctgcgtgt cgaggagtcc cgggcgggtc accgtgcccg gcgtgaccac
    13561 ctcgatgacc tggcgctcga cgagcccctt cgcctcggac gcggcttcca tctgctccgc
    13621 gatggcgacc gagtatccgt gctggacgag cttcttcaga tagctctcga ccgcgtggtg
    13681 cggaacgccg gcgagcggga gaggatcgcg cttcttgtcg cgggatgtca gggcgatgcc
    13741 gaggacgcgc gaggcgatga gggcgtcgtc gccgaacgtc tcgtagaaat cgcccatccg
    13801 gaagaggagg atgcagcctg gatgctccgc cttgagccgg cggtactgat ccagcatcgg
    13861 ggtcgattcc acggacccct ctagcgcgga acgatggtgt aggagaagcc gcgctcccgg
    13921 cggagtcgct cgcccatggc cttcgcctcg tcgcgcgtgg tgaacgcgcc cgcgcgcaca
    13981 cggaagaccg gcggcgtctc cggccccgcc ttcaccgaga catcctccac gcccgatgtc
    14041 ttgagatcct ttgcgagcga ttcggcgaga tcgcgccggc tgaacgcgcc gacctggagc
    14101 gcgaacccgc cgcgcaacgc gagcggtcct ggcgcctcgg gctcgaccgt gatgcggatg
    14161 ccgcgatcgc gcacccgcgt ggcctcgtag gaagcgggat agcggaccat cacctggcgg
    14221 aagacgccgg aggcgtcccc tgtgcgtccc accgcctcga gtcccgtgcc gagcgcgtag
    14281 agcgcctgcg gcacgtagtc ccctttcgga tgtccctcga gcagtcgctt gagcgcatcg
    14341 ccggcgtcgg ccggccggct cgcgtggagc gccgactgcg cgagcgcgaa gtcgtaggcc
    14401 tcctcctgcg ggctcccctc gagatcgggc gcggcctgat ggaggcccgc gagcgcgccg
    14461 acggcatcgc cgcgcgcgag ctgggcgagc ccgaggagga cgagcgcctg acggccgacg
    14521 aaatccttct ccacgccggg cgagaaggtg gcgagcagcc gctccgactc cgagtactcg
    14581 ccccgcacat accgcacctt cgccagggcg agcgtggcct cgcgccggtg gagtcccttc
    14641 ggatagtcgc gcaggtagtt ctggagcgct tcctggtagg cgtccccgtc ctcgatgagg
    14701 acggccgtga gataggcgaa gtcgtcttcc ttctcatggg gcacgcggcc gctcgtgacg
    14761 ggcgccaagt agcctcgcgc tccttcgacg ttgccatccg cgagggcttc gcgcgcgagc
    14821 gcgatcgccc gctcctccgg cgacggacca cggttcggcg cgggcttcga agccgtcttc
    14881 gcgcccaggg aagccgcggc cagcgcgagc acgagggcgg ccaggatgcc gcgcagcgcg
    14941 agcgaacgca ccggcaggct cacgcgcgcg cccatgcgaa gcactccgga caccgcgacc
    15001 agtactcctc ggactcgtgg ccgcacgacg ggcagatgta cacctccccc ggaccgaagc
    15061 gctcgatcag ggactgcatc tcgtcgagcg cgcgcgggag gtcgccgcga cgccggtgga
    15121 gctcggcgag gaggagccgc gccggcaccg agccgggctc gagctcgaac gcctcgttca
    15181 gcatgcgtcc ggcgtcgtcc aggtcgccct tcttgagatg gacccgcgcg agcgcgagat
    15241 ggacccgcac gtccttcgga ttccgcgcga gcatctcctc gtacgtctgg tccacgtcgg
    15301 cgaaccgccc cgattcgaag taggaagtct cgaggcggtc gagcacgagg tgcgaaaacg
    15361 tcggatgcgc ctccgccagc gccttccaga ggaccacggc gcgctccggc cggttcgtct
    15421 cgtagcagag atcgccgagg cgaaggagcg cggccggctc gtcctgcttc agcttgagcg
    15481 ccgcctggaa ctggcgcttg gcgtcgtcca gcttcccctc gcggagatag atctcgccga
    15541 tggacgagcg atagcgcgcg agcccgacgc cgttctcgct cgtcgccttg gccacctcgg
    15601 cccgggtctc gtatgcgcgc tcccattcgc ctgcggcctc gtgggctttc atgaggagct
    15661 tgagcgcgct gccgttcttc ttgtccaggt ctcggagttc gcgcgcttcg gcgaccgcgt
    15721 ccgccgggcg cccgagggcg atgagatcgc gcacgagccc ctcgcggatc gcgcgctcct
    15781 gccccgcgct caatccggcg cgcacggtca ggtcgcgccg gatctggagc gcgcgcgccg
    15841 cgtcgccgcg ctcccggagg aggttggcca ggtggatgta ggcatcgatg ttgtcgctgt
    15901 cctggcgcac ggtctcggcg aaggcgctga gcgcttccgc tcggtccccc gcgaggagcg
    15961 cgttcagcgc gtgctggtac ggcgtctcgg gcggaggcgg cgacgcgccc gcgtcgcgtg
    16021 acagaaccca cgcggcgtag ccgatcccgg tcaggagacc gccgacgacc aggacggtgg
    16081 cgaactcgct cattcgcggg cggcccctgc cgtctccttc agcgtgagat cctccagagg
    16141 caggttgcga agcccgtcca gctcgcgttt cagatcctga ttctcacgcc ggacccgcgc
    16201 gacgcccgcg cgggtcttga gctggtgcgc cagcgtgagc aggaacgcgg tgagcatccc
    16261 gatcacgaag gcggagaaca gcgcgacgtt cgacggaagc gtgcgataga tgcgcccggg
    16321 caggatcacc gaggtgaccg tctcgttcac gttcaggatg gcgaacccga caaccagaat
    16381 catgatgatg agccagatca gattcttgag gagccacatg gtcgcctccc tgagcggtgg
    16441 ggcgccgccc gccggggcgg cacggccgga gaatagcccg catggaactg ccggtcaacg
    16501 agttgcgagt tcgccaacgc atccgtcctg gcctgcctcg cgaaggacca cgcgcgcgag
    16561 cccgacccag cgcggtgtgg accgcacgag aaccggcgcg tacacatccg cgcgcccgcg
    16621 cacggaggcg gcatccgagc gcccctcgag gagcacggac cgcgtggtcc ccacgagcga
    16681 ctcgtagaac gcgcgttcct tggccagccc cagcgcacga agctccgccg cccgctcgcg
    16741 cgccgtcata ggcgccggaa gcggagccgc cgaggtcccc ggtcgggccg agtatcggaa
    16801 cacgtgtagg tatgagagcg gtgccccatc gatgaagcgg cccgtctcct cgaactccgc
    16861 gtccccttcc cccggatacc ccacgatgac atccgccccg atcgccaccg tcccccgcgc
    16921 ggtcacccgc tcgagcagcc gcgcgtagtc ccgcacccga tatcgccgcc tcatcgccgc
    16981 aaggatcgca tccgatccgc tctggagcgg caagtgaaga tgcggacaca gccgctcctc
    17041 gctcccaatc atctcgacca gccccgcagg caccttctcc ggctcgatcg aggagatgcg
    17101 cacgcgatgc gccgagccca acgcgaggag tcgctcgacg agaggtgcga gaagcccagg
    17161 cttcccgaga tcgctgccat acgatccgag gtccgccccg gtcacgacga tctcgtgaaa
    17221 ccccgcctcg agcagccgcg ccccttgggc gactgcttcg tccagaggaa ggctgcgtgc
    17281 ccgcccgcgc acataaggca ccacgcagta cgagcaaaac gcgtcgcacc cgtcctgcac
    17341 cttgagaagc gcccgagtac gcccgaacgc aacaggagag gaggcaacga acgcacgctt
    17401 cgcgcggccc ggcccgacgt gaagtgggat cgcatcgagc tgctcgtcga ggtaggaagc
    17461 gtggcctgga aaggcagagc ggccctcgcg ggcgtcgcgc gaagcgcgac gcaatgcgcc
    17521 ggccgcggcg cctttccagg ccgcgcttcc ggccccaaca tacaactcaa tgcgctcccg
    17581 ctccgccgtg cccagcacga gcgaaacgcc gggaatggcg gccacctcgc cgggcgcgcg
    17641 ttgcgcgtag cacccggtca cgacgatgcg agcggaaggc gcacgacgcg cgagcgatcg
    17701 cacgagctgc cgcgcctcct gatccgcgcg cgccgtcacc gtgcaggtgt tcacgaccac
    17761 gacgtccgca ccctcgggag cgtcgacctg agtccagccc gcatcagcga gccgggcccg
    17821 catggccgcc gtgtcgtgct gattcagccg gcagcccagc gtcaaaaacg aaacccgagg
    17881 ggcggaactc aacccctcgg gtgtctggcg atcccctcgg atcaccgagc tgctacgaag
    17941 ccgcgccgga gtcgctggtc tcctcggtcg cgaccggttc gttgtccgcg gacggcgtgg
    18001 gcttgtactt ctccgcgaac gcggcctgcg tggccgcgtc ctggtcggtg agccacgcct
    18061 tcacgctcag gatgattcga tgcgcctggg tatcgacccg ggtcaccttg aggtcgagcg
    18121 tgtcctccgg cttgaagctg tcggagggct tcttcaaccc ttcgatgccg agctgcgaga
    18181 gcggaatgaa accctcgagc ccgtcctcga gatccacgat gacgccgcgg tcgaggagac
    18241 ggaccacctt gccccggacg atcatgtcgg gaaggtagcg ctccgccagg gtcggccacg
    18301 ggtcgtcgct gacctgcttc aggccgagcg agatccgccg cgcgtccttg tcgatcgaga
    18361 gcaccacgac ctcgaccttg tcgcccttct tcagaacttc gctcggatgg accacgcggc
    18421 gcgtccacga catgtcgctg atgtggacga gaccgtcgat cccctcctcg atctcgacga
    18481 aggcgccgaa attggtgagg ttccgaacct tccccgtgag gcgcgtgccg atcgggaagc
    18541 ggtcgtcgag cgtgagccac ggatcgggct cgatctgctt caggccgagc gagatcttct
    18601 cgttgtcctt gtcgatcttg aggatcatcg cctcgatctg atcgccgatg gcgaccacct
    18661 tggacggatg ccgtacgtgg cgcgtccagg acatctcgga gatgtggatg aggccctcga
    18721 tccccttctc cagctccacg aaggcgccgt agtcggtgat cgagacgacc ttgcccttga
    18781 cccgctgggt gaccgtgtac cgcttgtcga cgtcttccca cgggtagggc gtgagctgct
    18841 tcatgccgag ggagatgcgc tccttctcgg gatcgaacga gagcaccttc acgcgcacct
    18901 tgtccccgat cgagacgagc tcggacgggt ggctcacgcg gccccaggac atgtcggtga
    18961 tgtggaggag gccgtcgatc ccgcccaggt cgacgaaggc gccgaagtcg gtgatgttct
    19021 tcaccacgcc ctcgcgaatc tgatccttgg ccagctcctg gatgatctcg ctcttctgct
    19081 tggcgcggtg ctcttccagg acggcgcggc gggagacgac gatgttccgg cgcctcttgt
    19141 tcagcttgat gatcttgaat tccatggact ggccgatgag cgagtccacg ttctgcacct
    19201 ggcggagcgc gatctgcgac ccggggagga acgcctccac cccgtagaga tccacgacca
    19261 cgccgccctt gatcttgcgg accagcttgc cctcgaccag ctctccgttg tcggaggcgg
    19321 tcttgacgcg gtcccacacc ttgacgaagt cggcgcgctg cttggacagg acgaccaggc
    19381 cgtcctggtt ctcggtcttc tcgaggaaga cctcgatctc gtcgccgacc ttgacgcctt
    19441 ccagatcggg gaactcctcg accggaatga cgccctcgga cttgaatccg acgtcgacgg
    19501 tgacttcctt gtcgtcgatc ttcaggacgc gcccgcggac gatctcccct tcctcgaggt
    19561 ccttgaggga atcctcgtag agttggagcc actggcccat cgagctctgg gcgtcctcgt
    19621 cgggatcgag ctcaccgact ccgaccgcta caccgccggc ctcctcgccc aagtcctcgt
    19681 gtaccagcct cgtgatggcc ttccgcttgc gcggcttggc ctcgacctcg gcgtcgtccg
    19741 ccgcgtcgtc cttcaggtcc acatcctcat cgtgccgtct caccatctgt gtctgtttcc
    19801 cccttgtttc tatgaaattc ggcctcgagg gccggtggtg ccgttcgtta ggcgcccgcc
    19861 gcttgcgcgg gctcgatctg ggtgaggcga tcgacgactt cggcgatcac ctcgtcgggg
    19921 gtggatgccc ccgcggacac gccgatctgg cgcatgccct ggaaccagtc ggggacgatc
    19981 tcctccgccg actcgatctg gtagacgttc gggttcacct tgcggcaaac ctccgcgagc
    20041 tgcgcggtgt tcgcgctctg ccggccgccg atgatgacca tcgcctcgac ggacggagcc
    20101 agctccaccg cggtcttctg catcgaggtg gtcgccccgc agagcgtgtt gaacacccgc
    20161 acttccttcc cgcgcttgag gagcgcgccc acgatctcct ggacgtgccc gatcgcgtgc
    20221 gtggtctgga acaccacgcc catccgcggg atgaacttga gcgcctcggc ctccgccacc
    20281 gtgtcgatga cgatggcctt gccgcccgtg tgcgcgatca ccccgatcac ctcggggtgg
    20341 tgctggtcgc cgatcatgac gaccttgtag ttctcgtcga tcagcttcgc cgcgtactgc
    20401 tgcgccttcg tcacgaaggg gcaggtcgtg tcgacgacct tgagccctcg cgtctcggcg
    20461 cttgccttca cctcgggccc gacgccgtgc gagcggatga tcacggtcga gccctggtcc
    20521 acctgctcga gctcgctcac cacctcgagg ccgcggttcc ggaacgacgc cacgacttga
    20581 ggattgtgga tcagagggcc cagcgccttg acgctcgccg ggtcctggtc catcgcctcg
    20641 ttcgccatct tgatcgcgcg cttcacgccg aagcagaagc ccgcgttgtc ggctacgatc
    20701 acttccacgc tagtacgctc ctcctggaag atccccgatg gcgtccatca cccgtcgcgc
    20761 gaacgcgtcg gcatcggcac ggctttcgac ctgccccacc gggggcatga tcatggagcc
    20821 gaaggccagc ctgatttcga cctgtcgcgc catggatcgg cggatctgtc tcgatcccga
    20881 gatgcgggcg ggtagaaccg gagcacgggc cgtcgccgcg aggcgcccga ttccggcgcg
    20941 cggcggcagg aacgacccgg tcttgctgcg ggtgccctcc ggaaagatca gtacggctcc
    21001 accttcatcc agcacccgct cggcgccgcg aagcgcggac cgggccccag gccctcgctg
    21061 gatcgggatc gaattatagc cccggatgag ggctccgaac cacctattct ggaacagctc
    21121 ctccttggcc aggaagagga gcctcctcgg gaccacgccc cccatgatgg gtgggtccca
    21181 gaaggagatg tggttgcagg cgatgatgac cggccctttg gccgggatgt gctccatccc
    21241 ggtgatccgg agcctcagga aggtccggca gaagatcccc gacgcgtggt gcccgaagag
    21301 gtagaagagg gtccgtttcc cgggcccccg ccgggccagc tcggcccagc ggaccgccag
    21361 atccacctgc tgctcgagcg tgagcgaggt cgtgtccagc gggaacgcgt ccggggcggg
    21421 cgcgaggggg ctcgcctccc gggcgctgtc cctccggtcg cgctcgcgga tggcggcctc
    21481 gatctccgcc gggtcggcgg gaagcccggc tgcccggagc tgcgcgaccc gccgctcggt
    21541 gcgcgcttcg gccgatgcgg tcaggaagat cttgaggtcc gccttcggga acacgacggt
    21601 cccgatgtcg cgcccctcca tgaccagccc gggcgctttg gcgagcgagc gctggatctt
    21661 gacgaggtgc ttccgcagct tgggatggac cgcgacctgc gaggccgcgt cgcccgcctc
    21721 cgcggtccgg atgtgcggct cgaccagctc gcctccgatc gtgagcccct gagggcccgc
    21781 ctc
//
LOCUS       DASTKC010000039         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_264927, whole genome shotgun sequence.
ACCESSION   DASTKC010000039 DASTKC010000000
VERSION     DASTKC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264927"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /gene="dnaG"
                     /locus_tag="VFR25_01545"
     CDS             complement(<1..900)
                     /gene="dnaG"
                     /locus_tag="VFR25_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET9939763.1"
                     /translation="MTTTRTPQEAKEEVRNAIDIAQVIGERVPLRRAGRTWKGLCPFH
                     AEKTPSFTVNPERQIWHCFGCNKGGDVFAFLMEIDKVTFPEALQALAERAGIDLPRSE
                     ASAGDKRRDRLYQATSLAKDFFEANLYSEAGAHARQYLAGRGFDRPMLERFHIGYAPA
                     GWDGLKTALGKLLPIEVLEEAGLVVRRGDGGHYDRFRNRITFPIEIAAGRLAGFGARA
                     VDPEDTPKYLNSPESPIYRKGSVLFGLTLARTAIREKRQVLVCEGNLDVVRLHAMGFA
                     HSVCTSGTALTIDQARALARFDAA"
     gene            complement(902..>2896)
                     /locus_tag="VFR25_01550"
     CDS             complement(902..>2896)
                     /locus_tag="VFR25_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9939764.1"
                     /translation="RAITRALEPSGEVKDDASPELSRIRRRIRGLREKLATKLESILR
                     SLATPESFVTLREGRYVVAIPSSNRRALPGTVLGHSGSGASLFVEPREAADANSELSE
                     LGLDEAAEVDRILRDLSGRAHTHRAALGADFEALVRLDAAEAVIAWTRDGDGSIPELT
                     EERRMRLRGGRHPLLVERHRKGETGAPVPLDLELDPDTRMLLITGPNMGGKTVAMKTV
                     GLLSLLAMTGLPIPAAAGSVMPWLDRVICDIGDEQSILADVSTFLSHLRRVTEAIEHA
                     TERSLVLLDELGSGTDPVEGAALGQAVLETLLQRGALTIATTHHGMLKTFAHDAAGVR
                     NASMAFDEETLRPRFRIVVGVPGGSRAIQVAERYGMEPKVLDRARQLLPEGERDLNKL
                     LEELSRLREEARTERAALERAMLDLASRESELREMRDRLESERRERKQAELAARKDLL
                     RQLENQIDDYRKKLRADKKATPERLREARDLAKEMEAEIERETPRQAPVVRGEPVRQV
                     AVGDRVFVTSLQADAEVLTDPDSDGRVRVRIGGVTAMLPLAQLRRAEGQAGQPAAGAT
                     AAGPATQRKHAVVVEPIESKSEIDVRGLEADEAIRAAERFLEDAVMAGLPTARIIHGK
                     GKGILRERMKHWLSKNSLVKEFRLGEIREGGTGVTVVTLG"
BASE COUNT          392 a         1072 c          970 g          462 t
ORIGIN      
        1 cgcggcgtcg aaccgcgcga gcgctcgcgc ctggtcgatg gtgagcgccg tccccgacgt
       61 gcagaccgag tgcgcgaacc ccatggcgtg cagccggacg acgtccaggt tcccctcgca
      121 cacgaggacc tgccgcttct cgcggatcgc cgtccgtgcg agcgtcaatc cgaagaggac
      181 gctgcccttt cgatagatcg gagactccgg agagttgaga tatttcggcg tgtcctccgg
      241 atccaccgcg cgcgcgccga agccagcgag ccgcccggcc gcgatctcga tggggaacgt
      301 gatccggttc cggaagcggt cgtagtgacc gccgtccccg cgccgcacca cgagccccgc
      361 ctcctccagc acctcgatcg ggagcagctt cccgagcgcg gtcttgagac cgtcccatcc
      421 ggccggcgca tacccgatgt ggaatcgctc gagcatgggc cggtcgaacc cgcgcccggc
      481 gagatactgc cgcgcgtgcg cgcccgcctc cgagtagagg ttcgcctcga agaagtcctt
      541 cgcgagcgag gtggcctggt agagccggtc gcgccgcttg tccccggcgc tcgcttcgct
      601 gcgcgggaga tcgatcccgg cccgctccgc cagcgcctgg agcgcttcgg ggaaggtgac
      661 cttgtcgatc tccatcagga acgcgaagac gtcgcctccc ttgttgcagc cgaagcagtg
      721 ccagatctgc cgctcgggat tcacggtgaa ggacggggtc ttctccgcgt gaaacgggca
      781 gagccccttc cacgtgcgcc cggcgcggcg gagcggcacg cgctcgccga tcacctgcgc
      841 gatgtcgatc gcgttccgga cctcttcctt cgcttcctgg ggcgtgcggg tcgtcgtcat
      901 gtcagccgag cgtcaccacc gtgacacccg ttccaccctc gcggatctca ccgagacgga
      961 actccttcac cagggaattc ttcgagagcc agtgcttcat gcgctcgcgg aggattccct
     1021 ttcccttgcc gtggatgatg cgcgccgtcg ggagcccggc catgaccgcg tcctcgagga
     1081 agcgctccgc ggcacggatc gcctcgtccg cctcgagtcc gcggacgtcg atctcggact
     1141 tggactcgat cggctcgacg acgacggcat gcttccgctg cgtcgccggt cccgcggccg
     1201 tcgcgcccgc cgcgggctgc cccgcctggc cctcggcgcg ccgaagctgc gcgagcggga
     1261 gcatcgcggt gacgccgccg atgcgcaccc gaacgcggcc gtcggaatcc ggatccgtga
     1321 gcacctcggc gtccgcctgg agcgacgtca cgaacacgcg gtcgcccacg gcaacctgac
     1381 gcacgggctc gccgcgaacc accggcgcct gccggggcgt ctcgcgctcg atctcggctt
     1441 ccatctcctt cgcgagatcg cgcgcctcgc gcagccgttc cggcgtcgcc ttcttgtcgg
     1501 cgcggagctt cttccggtag tcgtcgatct ggttctcgag ctggcgcagc aggtccttcc
     1561 gcgcggcaag ctcggcttgc ttccgctcgc gccgctcgga ctcgagccgg tccctcatct
     1621 cgcgaagctc cgactcgcgc gaggcgaggt cgagcatggc gcgctcgagc gcggcgcgct
     1681 cggtgcgcgc ctcctcgcga agccggctca gctcctcgag gagcttgttg agatcgcgct
     1741 ccccttcggg aaggagctgc ctcgcgcggt cgagcacctt gggctccatc ccgtagcgct
     1801 cggccacctg gatggcgcgg cttcctccgg ggacgccgac cacgatccgg aaacggggcc
     1861 ggagcgtctc ctcgtcgaag gccatcgacg cgttccgcac gcccgcggcg tcgtgcgcga
     1921 acgtcttgag catgccgtgg tgcgtggtcg cgatcgtgag cgcgccgcgc tggagcaggg
     1981 tctcgagcac cgcctgcccc agcgccgccc cctcgaccgg atccgtgccc gacccgagct
     2041 cgtcgagcag caccaggctc cgctcggtcg cgtgctcgat cgcctccgtc acgcgcctca
     2101 ggtgcgagag gaacgtgctc acgtcggcca ggatcgattg ctcgtcgccg atgtcgcaga
     2161 tcacgcggtc gagccacggc atcacgcttc ccgccgcggc cggaatcgga agtccggtca
     2221 tcgcgaggag cgagaggagg ccgaccgtct tcatggccac cgtcttcccg cccatgttcg
     2281 gcccggtgat gaggagcatc cgcgtgtcgg gatcgagctc gagatcgagt ggcaccggtg
     2341 cgccggtctc gcctttgcgg tgtcgctcga cgaggagcgg atgccgcccg ccgcggaggc
     2401 gcatacggcg ctcttccgtc agctccggaa tcgagccatc gccgtcgcgc gtccaggcaa
     2461 tcaccgcctc ggcggcatcg agccgcacga gggcttcgaa gtcggccccg agcgccgcgc
     2521 ggtgcgtgtg cgcccgaccg ctcagatcgc ggaggatccg atccacctcg gccgcttcgt
     2581 ccagccccag ctcggagagc tcgctgttgg catccgcggc ctcccgcggc tcgacgaaga
     2641 ggctcgcccc cgatcccgaa tgcccaagca ccgtgccggg aagcgcgcgg cgattggacg
     2701 acggaatggc cacgacgtac cgcccctcgc ggagcgtgac gaagctctcg ggcgtcgcga
     2761 gcgagcgaag gatcgactcg agcttcgtcg cgagcttctc gcgcaggccg cggattcgcc
     2821 ggcgaatcct ggacagctcg gggctcgcgt cgtccttcac ctcgccgctc ggctcgagcg
     2881 cgcgcgtgat cgcgcg
//
LOCUS       DASTKC010000040        14086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_737787, whole genome shotgun sequence.
ACCESSION   DASTKC010000040 DASTKC010000000
VERSION     DASTKC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737787"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFR25_01555"
     CDS             <1..729
                     /locus_tag="VFR25_01555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9939765.1"
                     /translation="ATEDGSFYYVMELLDGLDLRRLIERHGPVPVHRAVHLLAQACHS
                     LADAHESGLIHRDVKPANIFACRRGREEDFVKVLDFGLVKHADSKTVDVQLTAEGTTT
                     GTPAFMAPEAVYEADRMDARSDLYALGCVGYWLVTGRLVFERDTPIQTLLSHLNDTPV
                     PPSKRATQPIPEQFDRVILDCLEKDPAKRPESAETLGKRLQRSLGAMDPWTVDDARAW
                     WERVRHGSGEARSSPAEALSTAKS"
     gene            844..2283
                     /locus_tag="VFR25_01560"
     CDS             844..2283
                     /locus_tag="VFR25_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET9939766.1"
                     /translation="MRRFLLVALATARLACIAASAIASETAPPSETVLAQSRASSASM
                     GVECPECNPPKKFLPAFGELMAVQLIPWSVNHFARDAEWADISPETWWTNMSNPWLWD
                     NNKFVNNQFSHPYHGNLYFNAGRANGYNFWESSLWAGGGSLMWELYGEAWAPSPNDWF
                     NTTLGGIALGEMLWRMSSLALDNETSGSERTWREIGGAALNPVRGFSRLVNGQTHGRR
                     PNPDEWRPSRIQAVFDVGFRRTNHFGQTGEELDQGYGELALSYGDQVEDLKGKPFSAF
                     LVDLEVAPGQPEVGALAQLRARGNLAAHTLGRTNGHVHQLAAFLTYEYFNTPAFQFGG
                     QGFHGGLVSRWGKPRGARTQVEVLGSFLPISALRSDYFVTLEGRDYDYGVSFGTWTRV
                     ARIWEGKGMVQAVGRMLYTPIISGFDGDHVQAGATAEGRLYLRGRLGFGAAYTYYYRN
                     SWYDDFPDVKRDGSQARAYVSYAFPRWQP"
     gene            2280..2708
                     /locus_tag="VFR25_01565"
     CDS             2280..2708
                     /locus_tag="VFR25_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939767.1"
                     /translation="MTAASIRRVLGAAALGLATLLAVAGCQDTIVKYLGPENKPDLYI
                     ATDTTRYEAHDLDNVTDVEAWTWPHTGRIANVYHRNFVHHGHARITIEDANGKVVYDH
                     IPVEYSLVNETQDGEPGDWRVMIELFGAKGRVDFSVSKKR"
     gene            complement(2721..3863)
                     /locus_tag="VFR25_01570"
     CDS             complement(2721..3863)
                     /locus_tag="VFR25_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9939768.1"
                     /translation="MTSLKSAALAVSLGAALVAASPQPTAITSGPRIHHRGDTERSGS
                     YHGAPIRSRPRVLWTVDKGDLFLGTPLVDQGAIYSGASDGTLCAFDARTGKSLWTASG
                     FDMIENATAIAGDVIVGGGMNGQVRALNRKTGEPIWSYSAGVFVTTPPLIVGDAVYVA
                     THQGLHALDVGTGQRRWMTPLGTQPNFVGNPAAHKGVIVVSVGRELLAIDAATGAERW
                     RVRSSSQFWSPALGEDRVFVGSSDGNLIAYDLATGAERWRFRSAYPTADDIWSAPAIS
                     KGVVYAGSRDAALYAVDATTGRKVWSFPTTGESVGDPVVSNEILYFSDSNHALPPGVR
                     RLYALDASSGAEVWKYEVNSTLLTTPAVDQRTLYATITGQVMALTD"
     gene            complement(3961..6705)
                     /locus_tag="VFR25_01575"
     CDS             complement(3961..6705)
                     /locus_tag="VFR25_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9939769.1"
                     /translation="MLRSPSRLIPALALGALLAGCGSAPSVVYRVTISPATPHAIDVS
                     SEWTAVPRDSLVLGGFETADHLKISRFEATDLSGAALPVRSTVGGMTAEGRTLLVPRY
                     VIVGPLPSRIRVRDLIDPDIREGDAHVGFSSVRYGYVGQQFAEIGGRNLFLLPMGPLG
                     DVRVRFELPPGWIPVTPWAKKGGEFQPGIQGRFRDEHLIASTLAFGPFVERSMTLGKT
                     RYRFAYLPGSVDSTLTAFEHVARTVRAVVGGDLGREYVTAVLPTTPDGSDIHGEPWAT
                     GQGGTLTPITASRLLRYAEALLQARLKFEPYRSEIERPDEYWLVDGITNLYAWRAVAA
                     AGLVQESEIENDLATSYAAARHVAGVEHDLERLYETKLDTELARRVEAPYVLHFLDGE
                     LRARSGGRETLDAVVRRMLRSRRAPSLWTSFEGGGHRDWDRFRDRFVRGNEAIPPRRE
                     TGLALVEPTPTPPVGRPVHDLTVLFTGDTSGFLEHCGCKVNQSGGVARRATMIERLRR
                     EHPDAPLLDLGNAFAKPETPAELDYLARQEQRLYLQTMATMGYDAVAVGTNELLYGTG
                     WFREATKGLGLPYLSGNVLEHGATLEPAWRVVRARGLRIAIVSVLEPPHGPDALPQFE
                     ASTTSLRFADPVAAVERSLSEIGDRADFTIVIGRLESRTIQNLVLEVPGIDLILSNAG
                     GTSGLVQASGAPETTGDQGFLRRTLILHEDSRNYGLESVDLRLDDSSHVASARTTHHW
                     LYEDVPDDPTIRAMLTRFYEQVGTRDSAQASVRPLFPTSKERLEGIYVGASRCATCHR
                     LEFDQWKTTRHATAYKTLLDAHRHYQPRCIVCHVVGFRTAHGYKLGDPEEPLANVQCE
                     VCHGPGGAHVAAPATARMERTPPESTCLECHNPQHSDAFVYSEKIRMVQHRAEVASTP
                     "
     gene            complement(6758..7033)
                     /locus_tag="VFR25_01580"
     CDS             complement(6758..7033)
                     /locus_tag="VFR25_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939770.1"
                     /translation="MTSRLKLGALVGVLVLAMMLVGMFLSTGSKSVAKGPYQSALQSV
                     GVATAEAAKCANRACEFASPGFVCSEGSGTKCVFGGGCTTVSCSHGH"
     gene            complement(7161..8453)
                     /locus_tag="VFR25_01585"
     CDS             complement(7161..8453)
                     /locus_tag="VFR25_01585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9939771.1"
                     /translation="MTTTRADVIVVGAGAAGLEAARQLRAHGRSVLVLEARDRIGGRV
                     MTLNDPRIPLPIELGAEFIHGDAPITTALLAEAGQSSLDVSGPHGEAHRGTVRRVAEA
                     LSVDRVLHRIDTHAPDESVAEFLAQRPGGHALERERSQTRQFVEGFHAADITRISAQS
                     IAPDPGESGAELAAHVGRPTQGYHALMAWLARDLGSSLRLESTVRAITWGQRKVAVLV
                     RGPAGRKRRFTGRAAIVTVPIGVLQAPATAAGGIAFDPEPRHMRRAIAGIEMGRVARV
                     VVWFHSFPWDGASRFQFVHLHDGPFQVLWTAHPIRWPVAVLWCGGPTSARLEGSRDQV
                     QRVVETQLAAAFGTKPKQIRGGIRGLWWHDWSKDPYARGAYSYVQAGAKNPGRALSRP
                     EGRTLFFAGEAAGTDTGTVEAALESGRRAARQVHRALP"
     gene            complement(8450..9025)
                     /locus_tag="VFR25_01590"
     CDS             complement(8450..9025)
                     /locus_tag="VFR25_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939772.1"
                     /translation="MIPALTHWESFYVIVGSSAAALTGLQFVVITLIADSTMSVSGRG
                     LAAFGTPVVVHFGSALLISAVLSAPWTSTTPVAWTTAILGLGGLAYGAVVIRRARAQG
                     EYQVVTSDWIWHTVLPNFAYATVLLAALALTRSTEAALFVLGGAALLLLFIGIRNSWD
                     TVTYIVITFRRRHEGPEPAPTDEDQAVPPTE"
     gene            9201..10493
                     /locus_tag="VFR25_01595"
     CDS             9201..10493
                     /locus_tag="VFR25_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9939773.1"
                     /translation="MSSTPAATLRAHAQTTFRALRHRNFRLYWSGQLVSLIGTWMQTV
                     AQAWLIHRLTSSAFMLGLLGFMQFLPVLLLSLWAGVIADAMNKRKLLLITQTAFLVQA
                     TLLATVVSTHVVTAWMVLVLAFVFGTINALDLPARQSFVVELAGKEDLPNAIALNSAA
                     FNTARILGPAVAGILLATIGEAGCFWLNAVSYIAVIASIWRMDLMSRPAIKLDMSEAT
                     RHMKEGIRYVRSVLPLRNLLTLLGLTAGLGFQYMILLPVYVKDILHADAKAYGALVSA
                     FGLGSLISAAWMTRPHDRWALRRNIFIGLITASIGMGTFAWSRTLPLSLAMGFLAGLG
                     LILYVATTNTLIQLTTDDQHRGRVMGLYTLMFIGTAPIGSLITGSVAQKFGAPVATSI
                     SALVLFGGAAWMFRRLRVLALREAPPPAMAPGATEKLG"
     gene            10535..13147
                     /locus_tag="VFR25_01600"
     CDS             10535..13147
                     /locus_tag="VFR25_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939774.1"
                     /translation="MRGAAALGVALLLGMSLLASSPLSAQEAPAAPDSIAAPESTAAP
                     STAATATKTIVVDPKYKASGFHSFWLGEGYRKVWTTPVTAPVLDLQTYAGGLKPVREV
                     GARQTPGLALVGPDGRAYTFRSLRKEPDRALPEPYRKTWVAWVVRDHTAAQHPGAALL
                     LPPLAEAAGLLHTEPTLCVMDDDPALGSFREVFANQPGTIDEYPLAASGVPPFHGATE
                     IINTRKLWNRWLQGPENRVDSRAFLRARILDLYVENWDRHRGQWRWVRLPGNERWEPL
                     PEDPDMAFNRNDGFATSFLRSRNPKLVQFNDHFDSRLEGPTINGSEMDRWLLTDLDRA
                     AFEEVARETASKFTDDVIEQAVRRLPVEWQSLSGDLSSSLRARRGLLVGYVMHYYKDL
                     AKDVDVHATDKDEVVSIRRLDGGVVDVSVSLVGAEPYFERRFLPSETNEVRVYLHGGN
                     DRVERSGPKGGSILVRVIAGQGDDVVDDSKSGGTDVWSGSGALAIQKGRGTDTHREWR
                     NPEPDTTALWLEPRNWGRWTMLETQVAYGGDLGFVPEVTVLRSNWGFRTLPEARRQHL
                     SLAWSTGVSRGRAAYQGIFRRPGSGTAFRLDLLASGIEQFNFFGFGNETVLPARVEEH
                     RTEEQVLSIQPSVQIDPSHRLQLYAGPTYRGSWSPTDRENVLNAVSPYGLGQFSEAGL
                     RAGLDYDSRGQERASSLLEEILKGPAVASTWRLQGFYVPAVLDVDRPFGGIEGDVVSY
                     LGRNESRAQLAVRAGGRQVWGDYPWFESAFLGGRSSLPGYSRARFAGDKSLYGGLEAR
                     MWLFSVSPFRVGALGLADVGRVWLDGQSSDLWHNSWGAGGMIQPIATNLFLTGAVAWG
                     HDQTKVYFATKSLF"
     gene            13159..>14086
                     /locus_tag="VFR25_01605"
     CDS             13159..>14086
                     /locus_tag="VFR25_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9939775.1"
                     /translation="MSLAVRLPCDEASIRAGRVDPRCESAGRWVLAAAILGSSLSFID
                     GTVVTVALPAIGRELGGAGADLQWVVESYTLFLAALLLVGGSLADLFGRRRIYSLGVI
                     LFTAASIACGSASTMTWLITARALQGVGAALLVPGSLALISASFDQSHRGRAIGTWSG
                     FSGITAAIGPLLGGWLVNHSWRWAFFLNVPIAVLVLVLLTRVPESRNRDVRGVDVPGA
                     ALASLGLGGIVFALIESPRRGGADPAVLAGFGAGLTALAMFLLVEARSKSPMLPLSLF
                     RSRTFAGTNVLTLFLYGALSAVFFFLPLNLIQV"
BASE COUNT         2265 a         4709 c         4697 g         2415 t
ORIGIN      
        1 gccaccgagg acggaagctt ctactacgtg atggagctcc tcgacggcct cgacctgcgc
       61 cggctcatcg agcgccacgg tcccgttccg gtccatcgcg cggttcactt gctcgcgcag
      121 gcctgccatt cgctggcgga cgcgcacgaa agcggactca tccatcgcga cgtcaagccg
      181 gcgaacatct tcgcctgccg gcgcggacgg gaagaggact tcgtcaaggt cctcgatttc
      241 gggctcgtga agcacgcgga ttcgaagacg gtcgacgttc agctcaccgc ggagggaacg
      301 accaccggaa cgcccgcgtt catggctccc gaagccgtct acgaggcgga ccggatggac
      361 gcgcgctccg atctctacgc gctcggctgt gtcggatact ggctcgtgac ggggcgcctc
      421 gtgttcgagc gcgatacgcc catccagacc ctcctgagcc acctcaacga cacgcccgtc
      481 cctccatcga agcgggcgac gcagccgatt cccgagcagt tcgaccgggt gatcctggac
      541 tgcctggaaa aggatccggc caagcggccc gagtcggcgg aaacgttggg aaagcgactc
      601 cagcgttccc tcggcgcgat ggatccatgg acggtggacg atgcgcgcgc ctggtgggag
      661 cgggtgcgcc acggttcagg cgaggcgcgc tcgagcccgg cggaagcgtt gagcaccgcc
      721 aagagctgac cctccccaga gtacagttcc gaaatcctac tgcggcattt ccgggcgtgt
      781 gctagcgtcg cgccgttcga acgatgcacg cgttccccgg atccagggat ggccggttcc
      841 cgaatgaggc gcttccttct agtggcactc gcgaccgctc ggctcgcgtg catcgccgct
      901 tccgcaatcg cgagcgagac cgctccgcct tcggagaccg tgctcgcgca gtcccgcgcc
      961 tcgagcgctt cgatgggcgt cgagtgcccc gaatgcaacc cgcccaagaa attcctgcct
     1021 gcgttcggtg agctcatggc cgtgcagctc attccgtggt cggtgaatca cttcgcccgc
     1081 gacgccgagt gggcggacat cagtcccgag acctggtgga cgaacatgag caacccctgg
     1141 ctctgggaca acaacaagtt cgtgaacaac cagttctcgc acccgtacca cgggaacctc
     1201 tacttcaatg ccggccgtgc caacggatac aacttctggg aatcgtccct ctgggccggc
     1261 ggcggcagcc tcatgtggga gctctatggc gaggcatggg ctccatcacc gaacgattgg
     1321 ttcaacacga cgctcggcgg catcgcgctg ggcgagatgc tctggcgcat gtcctcactc
     1381 gcgctcgaca acgagacctc tgggagcgag cggacgtggc gggagatcgg tggagcggcg
     1441 ctcaatccgg tgcgtggatt cagccggctc gtgaacggcc agacgcatgg gcggcgcccc
     1501 aatcccgacg agtggcgccc gagccggatc caggcggtgt tcgacgtcgg cttccgtcgc
     1561 acgaaccact tcggccagac cggcgaagag ctggaccagg gatacgggga gctcgcgctc
     1621 tcctacggcg accaggtgga agacttgaag gggaagccct tctccgcgtt cctcgtggac
     1681 ctggaggtcg cccccggcca gcccgaagtg ggcgctctcg cccagctccg tgcccgcggc
     1741 aatctggcgg cccacaccct cggccgcacg aacggacacg tgcaccagct cgccgcgttc
     1801 ctcacgtacg agtacttcaa tacgcccgcg ttccagttcg gcggacaggg gttccacggc
     1861 ggcctcgtct cgcgctgggg aaagccgcgg ggcgcgagga cgcaggtcga ggtcctcgga
     1921 tcgttccttc cgatctcggc gctcaggtcc gactacttcg tgacgctgga ggggcgtgac
     1981 tacgactacg gcgtcagctt cggcacctgg acccgcgtgg cgaggatctg ggaagggaag
     2041 ggcatggtgc aggcggtcgg gcgcatgctc tacacgccga tcatcagtgg attcgacggc
     2101 gatcacgttc aggcgggcgc gacggcggag gggcgcttgt atctccgcgg gcggctcgga
     2161 ttcggagcgg cgtacaccta ctactaccga aacagctggt acgacgactt cccggacgtg
     2221 aagcgcgacg gctcgcaggc ccgcgcatac gtgtcgtacg ccttcccacg gtggcagcca
     2281 tgacggccgc gtcgatccgt cgcgtgctcg gcgccgcggc cctcggtctc gcgaccctgc
     2341 tcgcggtggc cggctgtcag gacacgatcg tcaagtacct gggtcccgag aacaaaccgg
     2401 acctctacat cgcgaccgac acgacgcgct acgaggccca cgatctcgat aacgtgaccg
     2461 acgtcgaagc gtggacctgg ccccacaccg gccggattgc gaacgtctac caccggaact
     2521 tcgtgcatca cggccatgcc cgcatcacca tcgaggatgc gaacggcaag gtcgtctacg
     2581 accacatccc cgtcgagtac agcctcgtga acgagacgca ggacggcgag ccgggcgact
     2641 ggcgcgtgat gatcgagctc ttcggcgcga agggtcgagt cgacttctcg gtctcgaaga
     2701 agcgctagcc ttcgttcgcc ctagtcggtc aacgccatca cctgccccgt gatggtggcg
     2761 tacagcgtgc gctgatccac ggccggcgtc gtcaacagcg tgctgttcac ctcgtacttc
     2821 cagacctcgg ccccactcga cgcgtcgaga gcgtagagac gcctcacgcc gggtggaagg
     2881 gcatggttgc tgtcgctgaa gtagagtatc tcgttgctca caaccgggtc gcccaccgac
     2941 tcgcccgtcg tggggaacga ccacaccttc ctgccggtcg tagcatccac ggcgtagagc
     3001 gccgcatcgc ggctccccgc atagacgact cccttcgaga tggcgggagc cgaccagatg
     3061 tcgtcggccg tcgggtacgc cgaccggaat cgccagcgct cggcaccggt cgccaggtcg
     3121 tacgcgatca ggtttccgtc ggagcttccc acgaaaaccc gatcctcgcc gagcgccgga
     3181 gaccagaact ggctcgacga tcgaacccgc caccgctccg cccctgtggc ggcgtcgatc
     3241 gccaggagct cccggcccac gctcaccacg atcacacctt tgtgggcagc gggattgccg
     3301 acgaagttgg gctgcgtgcc gagcggcgtc atccaccggc gctgacccgt gcctacatcg
     3361 agagcgtgga gcccctgatg cgtcgccacg tagaccgcgt ctcccacgat caagggcggc
     3421 gtcgtgacga agactcccgc gctgtacgac cagatcggtt cgcccgtctt gcggttcaag
     3481 gcgcgcacct ggccgttcat gccgccgccg acgatcacgt cgcccgcgat ggcggtcgcg
     3541 ttctcgatca tgtcgaaccc ggatgcggtc cagagagact tgcccgtccg cgcgtcgaac
     3601 gcgcacagcg taccgtccga ggcgccgctg tagatcgctc cctggtcgac tagcggcgtt
     3661 cccaggaaga ggtcgccctt gtccacggtc cagagaactc ggggccgcga ccggatgggc
     3721 gctccgtgat aggagcccga gcgctcggtg tcgccgcgat gatggattcg agggccggac
     3781 gtgatggctg tgggctgcgg ggatgcggcg accagggcgg ccccaagcga gaccgcgagc
     3841 gctgcgctct tgagagatgt catttcgtgg tgctcctgcc cccggcggga cccgaggacg
     3901 atagcgaaac gcgcagcgcg cgacaatggc ggatggcgcc aggaggggct tgtcgtagcg
     3961 ctacggcgtc gaggccacct ccgcccggtg ctgcaccatg cggatcttct ccgagtagac
     4021 gaacgcgtcg gagtgttgcg ggttgtggca ttcgagacag gtggattcgg gcggcgtccg
     4081 ctccatgcgc gcggtcgcgg gggccgcgac atgggcgccg cccggcccgt ggcacacctc
     4141 gcactggacg ttcgccagcg gctcttccgg atccccgagc ttgtagccgt gcgccgtccg
     4201 gaatccaacg acgtggcaga cgatgcagcg gggttggtag tggcgatgcg cgtcgagcag
     4261 cgtcttgtac gccgtggcgt ggcgcgtcgt cttccactga tcgaactcca accggtggca
     4321 cgtcgcacag cgtgaagccc cgacgtagat accctcgagg cgctccttcg aggtggggaa
     4381 gagcggccgg acgctcgcct gggccgagtc ccgcgttccg acctgctcgt agaagcgcgt
     4441 cagcatggcg cggatcgtgg ggtcgtcggg gacgtcctcg tagagccagt ggtgcgtggt
     4501 cctggcgctc gcgacgtggc tcgaatcgtc gaggcgaagg tcgaccgact cgagtccgta
     4561 gttccggctg tcctcgtgga ggatcagggt ccgcctcagg aagccctgat ccccggtcgt
     4621 ttccggagcc ccggaggcct ggacgaggcc cgaggttcct cccgcgttcg agaggatcag
     4681 gtcgatcccg ggaacctcga ggaccaggtt ctgaatggtc ctcgattcca atcgcccgat
     4741 cacgatcgtg aagtcggccc gatctccgat ttcgctgagg ctccgctcta cggcggcgac
     4801 gggatccgcg aatcggagac tcgtcgtgct cgcctcgaac tggggcaacg cgtcgggacc
     4861 gtgcggcggc tcgaggaccg agacgatcgc gatgcgaagc cctcgggcac gcacgactcg
     4921 ccacgccggc tcgagcgtgg ctccgtgctc caggacgttg cccgataggt agggcaatcc
     4981 gagccctttc gtggcctcgc ggaaccatcc ggttccgtag aggagctcgt tcgtgcccac
     5041 cgcgaccgcg tcgtagccca tggtggccat ggtttggagg taaagcctct gttcctggcg
     5101 cgcaagatag tcgagctccg ccggcgtctc cggcttggcg aaggcgttcc cgagatcgag
     5161 caggggcgcg tccgggtgct cgcgcctcag gcgttcgatc atcgtggctc ttcgcgccac
     5221 gcctcccgac tgattcacct tgcagccgca gtgctcgaga aagccgctgg tgtcgcccgt
     5281 gaagaggacg gtgaggtcgt gcaccgggcg gcccacgggc ggtgtcgggg tgggttcgac
     5341 cagggcgagg cccgtttctc gtcgtggggg tatggcctcg ttgcctcgca cgaagcgatc
     5401 gcggaagcgg tcccagtctc gatgcccgcc tccctcgaac gacgtccata gggagggggc
     5461 gcgccgcgac cggagcatcc gtcgcacgac tgcatccaga gtctcgcgcc cgccgcttcg
     5521 cgcacgaagc tcgccgtcca ggaagtggag cacgtacggg gcctcgaccc gccgcgcaag
     5581 ctcggtgtcg agcttcgtct cgtagaggcg ctccagatcg tgctcgacgc cagcgacatg
     5641 gcgcgctgcg gcgtaggacg tcgcgagatc gttttcgatc tccgactcct gcacgagtcc
     5701 ggcggcggcg accgcgcgcc acgcgtagag attcgtgatt ccgtccacga gccagtactc
     5761 gtcggggcgc tcgatctcgc tgcgatacgg ctcgaacttg aggcgcgcct ggagcagggc
     5821 ttccgcgtag cgcaggagcc ggctcgcggt gatcggcgtg agcgtcccgc cctgtcccgt
     5881 ggcccacggc tcgccgtgga tgtcgcttcc atccggtgtg gtcgggagca cggcggtcac
     5941 gtactcgcgg cccagatcgc cgccgacgac cgcgcgcacc gtccgggcca cgtgttcgaa
     6001 cgcggtgagc gtcgagtcca cgcttccagg caggtaagcg aatcgatatc gagtcttccc
     6061 gagggtcatc gagcgctcga cgaatggccc gaacgcgagc gtcgacgcga tcaagtgctc
     6121 gtcgcggaac cggccctgga tgcccggttg gaactccccg cccttcttcg cccatggcgt
     6181 caccgggatc caccctgggg gcagctcgaa gcgaacgcga acatccccaa gcgggcccat
     6241 ggggagcagg aacaggtttc gaccgccgat ttctgcgaac tgctggccga cgtatccgta
     6301 gcgaacgctg ctgaaaccga cgtgggcgtc gccctcacgg atgtcggggt cgatcaggtc
     6361 gcggacgcgg atccgcgaag gaaggggacc gacgatcaca taccggggca caaggagcgt
     6421 gcggccctcg gcggtcatcc ctccgaccgt ggatcggacc ggcagtgccg ccccggacag
     6481 gtcggtggcc tcgaacctcg agatcttcag atgatccgca gtctcgaatc cgccgagcac
     6541 cagcgagtct cgaggaacgg cggtccattc ggagctgaca tcgatggcgt ggggcgtggc
     6601 aggcgagatg gtgactcgat agacgacgga cggagccgag ccgcagcccg cgagaagcgc
     6661 gcccagcgca agggctggaa tgaggcgtga aggggagcgg agcacggatt tcaatgcggc
     6721 gggccggcgg tgccgccggt ccgccgcgaa acgtcagcta gtgaccgtgg ctgcaggaaa
     6781 ccgtggtgca tcctccgccg aagacgcatt tcgtgccgct gccttcgctg catacgaagc
     6841 ccggcgaggc gaattcgcag gcgcggttgg cgcacttcgc ggcttcagcc gtggcgactc
     6901 ccacgctctg gagcgcggac tgatagggtc ccttggctac ggacttggat ccggtggaga
     6961 ggaacatgcc gaccagcatc atcgcgagga ccagcacgcc tacgagggcg cccagcttga
     7021 gccgtgatgt catgtctccg tcctttcgac cgatggggaa cggccgcccg agtggtcagg
     7081 ctcggacggg aggccggcgg caccaccggc gcccgcgacc cggaacgcgg aagcccgaag
     7141 catagcaccg gagccggacg tcagggcagc gcccggtgaa cctggcgcgc cgcgcggcgc
     7201 ccgctctcga gcgccgcctc caccgtgccc gtgtccgtcc ccgctgcttc gccggcaaag
     7261 aagagcgttc gcccttccgg tcgggacagg gcacgcccgg ggttcttggc tcctgcctgg
     7321 acgtaggagt aggcgccgcg ggcgtaggga tccttcgacc agtcgtgcca ccagaggccg
     7381 cgaatccctc ctcggatttg cttcggcttc gttccgaagg ccgccgcgag ctgggtctcg
     7441 accacccgct ggacctggtc gcgcgagccc tcgagtctcg cggaggtcgg tcctccacac
     7501 cacagcaccg cgaccggcca tcggatcggg tgcgcggtcc acaagacctg gaacggtccg
     7561 tcgtgcaggt ggacaaattg gaaccgcgac gcgccgtccc atgggaatga atggaaccac
     7621 acgacgactc gagcgacccg ccccatctcg atgccggcga tcgcgcgacg catgtgccga
     7681 ggctcgggat cgaacgcgat gcccccggcg gctgttgccg gcgcctggag cacgccgatc
     7741 ggaacggtga cgatggcggc tcggccggtg aagcgtcgct tccgcccggc gggtccgcgc
     7801 actaggacgg cgaccttccg ctgaccccac gtgatcgcgc gcaccgtgct ctcgaggcgc
     7861 agcgacgacc cgagatcgcg ggcgagccac gccatgagtg cgtggtagcc ctgcgtcgga
     7921 cgcccgacgt gcgcagccaa ctccgctccg gactcgcccg ggtcgggcgc gatcgattgg
     7981 gcgctgatcc gcgtgatgtc cgcggcgtga aatccctcga cgaactgcct cgtttggctg
     8041 cgctccctct cgagcgcgtg cccgcccggc cgctgggcga ggaactcggc cacgctctcg
     8101 tccggggcat gggtgtcgat gcgatgaagc acacgatcga ccgagagcgc ctcggcgacg
     8161 cggcgcaccg tcccccgatg ggcttctcca tggggaccgg aaacgtcgag ggacgactga
     8221 ccggcctctg ccaggagcgc ggtcgtgatc ggcgcgtcgc catggatgaa ctcggcgccg
     8281 agctcgattg gaaggggaat gcgcgggtcg tttagggtca tcacgcggcc accgatccgg
     8341 tcccgagcct cgagcacgag caccgagcgt ccgtgtgcgc gcagctgccg cgcagcctcg
     8401 aggcccgcgg cgccggcgcc gacgacgatc acgtcggctc gcgtggtcgt cattccgtcg
     8461 gaggaaccgc ctggtcttcg tccgtcggcg ccggctccgg accttcgtgt cgccgccgga
     8521 acgtgatgac gatgtacgtg accgtgtccc acgaattgcg gatcccgatg aacaggagga
     8581 ggagtgccgc tccgccgagg acgaagaggg cggcctccgt gctccgcgtg agcgcgagag
     8641 ccgccagcag gacggtcgcg taggcgaagt ttggaagcac ggtgtgccag atccaatccg
     8701 aggtcacgac ctggtactcg ccctgggctc gtgccctgcg gatcacgacg gctccgtacg
     8761 cgagcccgcc caatcccaga atcgccgtcg tccaggccac gggagtcgtc gaggtccagg
     8821 gagcgctcag gaccgccgag atgagaagcg ccgatccgaa atggacgacc acgggcgtgc
     8881 cgaacgcagc gagccctcgc ccggacacgg acatggtcga gtcggcaatc aacgtgatca
     8941 cgacgaactg gagcccggtg agcgcggccg cggaggatcc gacgatgacg tagaagcttt
     9001 cccagtgcgt cagggctgga atcacggtga agcctcccct gaaaccaacg gcgtccccag
     9061 gcatcgatga tgataagagc gcatggtggt tcgacacatc gtcgccaccc ttgctacgag
     9121 cgctgccagg ctgctaagtt aatagattca gagtacttgg caactccaaa ccatagcgga
     9181 gggctgagcc gatccccgac atgagctcca cgcccgccgc caccctccgc gcacacgctc
     9241 agaccacctt ccgcgccctc cggcaccgca atttccgcct ctactggagc gggcagctcg
     9301 tctccctcat cggcacctgg atgcagaccg tcgcccaggc atggctcatc catcgcctga
     9361 cctcgtccgc cttcatgctc ggtcttctcg gattcatgca gttcctgccg gtgctcctgc
     9421 tctcgctctg ggccggcgtc atcgccgacg cgatgaacaa gcggaagctc ctcctcatca
     9481 cccagacggc gttcctcgtt caagcgacgt tgctcgcgac cgtggtgtcg acccacgtcg
     9541 tgaccgcctg gatggtcctc gtgctcgcgt tcgtcttcgg cacgatcaac gccctcgatc
     9601 ttcctgcgcg ccagtccttt gtcgtggagc tggcggggaa ggaggacctc ccgaacgcga
     9661 tcgccctcaa ctcggcggcg ttcaacaccg cgcgaatcct cggtcccgcg gttgcgggca
     9721 tccttctcgc caccatcggg gaggcgggct gcttctggct gaatgcggtg tcctatatcg
     9781 ccgtgatcgc gagcatctgg agaatggatc tcatgtcgcg gccggcgatc aagctggaca
     9841 tgagtgaggc gacgcgccat atgaaggagg gaatccggta cgtgcggagc gtgctcccgc
     9901 tgcggaacct cctcaccctt ctcgggctga ccgcaggact cggattccag tacatgatcc
     9961 tgcttcccgt gtacgtgaag gacatcctgc acgccgatgc caaagcctac ggagccctcg
    10021 tctccgcgtt cggacttgga tcgttgatct cggccgcatg gatgacccgg ccgcacgatc
    10081 gatgggcctt gaggcggaac attttcatcg gcctcatcac cgcgtcgatc gggatgggga
    10141 ccttcgcgtg gtcgcgcacg cttccgttga gtctcgcgat gggatttctc gcggggctgg
    10201 gcctcatcct ctacgtggcc acgacgaaca cgctgatcca gctcacgacc gacgaccagc
    10261 atcggggtcg cgtcatggga ctttacacgc tgatgttcat tggcacggca cccatcggct
    10321 cgctcatcac cggctcggtg gcgcagaagt tcggcgcccc ggtggcgacg tcgatcagcg
    10381 cgctcgtgct cttcggcgga gcggcctgga tgttccgccg gctccgcgtc ctcgccctcc
    10441 gcgaggcgcc gccgcccgcg atggcgccgg gtgccaccga gaagctcggg tgaggcggtg
    10501 gcggggtgag cccacagcgc gtagtatcga tgtcatgaga ggcgccgcag cgctcggggt
    10561 tgctctgctc ctcgggatgt cgttgttggc atcgtcgcca ctatcggctc aagaggctcc
    10621 cgccgcgcct gactctatcg ccgcgccaga atccaccgcc gcgccgtcca ccgctgcgac
    10681 agccaccaag accatcgtcg tcgacccgaa gtacaaggcc agcggcttcc acagcttctg
    10741 gctcggtgag ggctatcgca aggtctggac gacgccggtt acagcgccgg tgctcgatct
    10801 ccagacctac gccggcggac tcaagccggt tcgcgaggtg ggcgcgaggc agacgcccgg
    10861 actcgctctc gtgggacccg acgggcgcgc ctataccttc aggtctctta gaaaggagcc
    10921 cgaccgcgct ctcccggagc cgtatcgcaa gacatgggtg gcctgggtcg tgcgcgacca
    10981 cacggccgcg cagcatccgg gcgcggcgct ccttcttccg cccttggccg aggccgctgg
    11041 cctcctgcac acggagccca cgctgtgcgt gatggacgat gaccccgcgc tcggatcgtt
    11101 tcgcgaggtc ttcgcaaacc agccggggac gatcgatgag tatccccttg cggcatcagg
    11161 cgtgcccccg ttccacggcg ccacggagat catcaacacg cgcaagctgt ggaaccgatg
    11221 gctccagggc cccgagaatc gtgtcgattc gagggcattc ctgcgcgcac ggatcctcga
    11281 cttgtacgtg gagaactggg atcgccatcg agggcaatgg cgctgggtga ggctgcctgg
    11341 gaacgaacga tgggagcctt tgcccgagga tccggacatg gcgttcaaca ggaacgatgg
    11401 attcgcgact tcgttcttgc ggtcccgaaa tcccaagctc gtccagttca acgaccactt
    11461 cgacagtcgc ctggagggcc ccacgatcaa tgggtccgag atggatcgtt ggctcctcac
    11521 cgacctcgac cgagcggcct tcgaggaagt agcccgagaa accgcgtcga agttcaccga
    11581 cgatgtcatc gagcaggcgg ttcggcgcct tcctgtggag tggcagagcc tctcggggga
    11641 tctctcatcg tccttgcggg cgcgccgcgg gctgctcgtg ggctacgtga tgcactacta
    11701 caaggacctc gccaaggacg tggacgttca cgccaccgac aaggacgagg tcgtctcgat
    11761 ccggcgactc gacggaggag tcgtcgatgt gagcgtgtct ctcgtcgggg ccgagccgta
    11821 cttcgaacgg cgcttcctgc cctcggagac gaacgaggtc cgggtctatc tccatggcgg
    11881 gaacgatcgc gtcgagcgta gcggcccgaa gggtgggtcg attctcgtac gcgtcatcgc
    11941 gggccagggt gacgacgtcg tagacgattc gaagagcggg ggaaccgacg tctggtcggg
    12001 aagcggcgct ctggccattc agaaggggcg cggcaccgac acacatcgcg aatggcggaa
    12061 tccggaaccg gataccaccg ctctctggct cgagccccgg aactggggac gctggaccat
    12121 gctggagacc caggtcgcgt acggaggcga tctcggtttc gttccggagg tcaccgttct
    12181 ccgatcgaac tggggatttc gtaccctgcc cgaggccagg cgacagcacc tgtctctggc
    12241 ctggtccacg ggcgtgagcc ggggccgcgc agcgtatcag ggcatcttcc gccgcccggg
    12301 ttccggcacg gcgttccgac tggatctgct cgcgtcgggg atcgagcagt tcaatttctt
    12361 tggatttgga aacgagactg tcctgcctgc ccgcgtcgag gagcatcgaa cggaagagca
    12421 ggtcctctcc atccagccct ccgtgcagat cgacccatca catcggctcc agctctacgc
    12481 aggacccacg taccgcgggt cctggtcgcc cacggaccgt gagaacgtct tgaacgccgt
    12541 ctcaccgtat ggcctggggc agttctcgga ggctggactg cgagccggac tggactacga
    12601 cagccggggg caggagcgag cgtcgtctct gctggaggaa atactgaagg ggccggccgt
    12661 cgcgtcgacc tggcggttgc aggggttcta tgtccccgcg gttctcgacg tggatcgtcc
    12721 tttcggtgga atcgaagggg acgtcgtgtc gtacctggga cggaacgaat ctcgcgctca
    12781 gcttgcggtc cgagccggag ggaggcaggt ctggggggac tacccgtggt tcgaatccgc
    12841 attcctcggc ggacgttcca gcctgcccgg gtacagccga gcccgcttcg ccggcgacaa
    12901 gtcgctctac ggtggcctgg aagcgcgcat gtggctcttc tcggtgagcc ccttccgcgt
    12961 gggtgcgctc ggcctcgcag atgtcgggcg ggtgtggctg gacggacaga gctccgatct
    13021 ctggcacaat tcctggggtg ccggcggcat gatccagccc attgccacga atcttttcct
    13081 caccggcgcc gtggcttggg gccacgatca aaccaaggtc tacttcgcaa cgaaatctct
    13141 gttctagggg ctagccgcgt gtcgctcgcc gtccgtctcc cttgcgatga ggcatcgatt
    13201 cgcgcgggtc gagtcgatcc tcgatgtgag tcggcggggc ggtgggtcct tgcggcagcg
    13261 atcctcgggt cgagcctctc cttcatcgac ggcacggtcg tcaccgtcgc gcttcccgcg
    13321 atcggtcgag agcttggggg cgcgggagcg gacctccagt gggtcgtcga gtcctacacc
    13381 ctcttcctgg ccgcgctcct cctcgtcgga ggatcgctcg cggatctctt cggcagacga
    13441 cggatctatt cgctcggagt gatcctgttc acggccgcat cgatcgcgtg cggctccgcc
    13501 tccaccatga catggctgat caccgcgcga gcgctccagg gggtcggggc ggccttgttg
    13561 gtccccggta gcctggcgct catctcagcc tcattcgatc agtcgcaccg gggtcgcgcg
    13621 attggcactt ggtccggatt cagcgggatc acggccgcga tcggaccctt gctgggcgga
    13681 tggctcgtga atcactcctg gcgctgggcc ttcttcctga acgtaccgat cgccgtcctg
    13741 gtcctcgttc ttctgacgcg cgtgcccgag agtaggaatc gcgacgtgcg cggcgtggat
    13801 gttccgggcg ccgctctggc cagcttgggc ctgggtggaa tcgtgttcgc gctgatcgag
    13861 tcgcctcgac gtggaggggc ggatcctgcc gttctcgcgg gtttcggcgc gggactgacc
    13921 gctttggcga tgttccttct cgtggaagct cgatcgaaat cgccgatgct cccgctctcc
    13981 ctcttccggt cccgcacctt cgcggggacc aacgtcctca cgctctttct ctacggggca
    14041 ctgtccgccg tgttcttctt ccttccgctg aatctgatcc aagtcc
//
LOCUS       DASTKC010000041        27233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_266652, whole genome shotgun sequence.
ACCESSION   DASTKC010000041 DASTKC010000000
VERSION     DASTKC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 27233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27233
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266652"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFR25_01610"
     CDS             complement(<1..213)
                     /locus_tag="VFR25_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939776.1"
                     /translation="MTPRLKKITLFVGAGLALYAVAGFLVAPRILRSVLLKNLGASLA
                     TTPTLGDVRVNPFAPSVTLRRFAIPDK"
     gene            complement(316..684)
                     /locus_tag="VFR25_01615"
     CDS             complement(316..684)
                     /locus_tag="VFR25_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939777.1"
                     /translation="MSFVGTYEPDDIIRQFETALAEAPPSEKIALLVDTTRSEVLGTR
                     SPNQIRYVAQALAPYSERIGGRVAVVATEDVHFGLSRMGSVYSESVGVEAEVFRNEED
                     ALAWLGGAGGPHVGVQRDRA"
     gene            complement(750..1139)
                     /locus_tag="VFR25_01620"
     CDS             complement(750..1139)
                     /locus_tag="VFR25_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939778.1"
                     /translation="MKRLSAAAWAAGLLLAAGCASTHTSGNDFQVTNRSDYFEFKIGT
                     AKTFTMSQNYAWWNPDSSAVVRQGSDIQGGEALVEVRDADGSVVHSKNLSEQGTMVSL
                     KGKPGMWSVKVTLDKASGLVRFQIEGK"
     gene            complement(1136..2377)
                     /locus_tag="VFR25_01625"
     CDS             complement(1136..2377)
                     /locus_tag="VFR25_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9939779.1"
                     /translation="MPRTSETIVIGAGQAGLSTSYLLSTHGREHLVLERGRVAETWRS
                     ERWDGFYLNTPNWCHKLPGFAYAGPDPDAFAALPEVISYLEDYARSFRAPVREGVEVL
                     AVRPDGSGFTVETNGGALTADNVVVAAGAYQRPTPTPLMAAVPSDVMQIHTSQYRRPS
                     QVPDGAVVMVGSGQSGCQIADELIDAGRQVYLAAGRCPWLPRRYRGGELMHWMLETGL
                     ADQTVDTLPSPAARLLCNVPVSGNDGGHDCNPRWLARRGAVLLGRLEGVDGWKLKIGG
                     GLEESLTMGDEFVAAYRKRVDDYVRAKGIHTADPEPEDPRDPISSPGELDLRQAGVST
                     MIWANGFRPEYRWIEGLELDQQGWPVHRRGVSTLPGLYFVGMHWLHKRKSALFLGVGE
                     DAEYVVEQIMERESAVKGGGA"
     gene            complement(2361..2807)
                     /locus_tag="VFR25_01630"
     CDS             complement(2361..2807)
                     /locus_tag="VFR25_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9939780.1"
                     /translation="MKTDALAIHLCLTAAFEPYRAQYTHGAFRDTVPSPAGIEERLRD
                     MALFVATAPDGAIVGTIACQDVGGGLGHLRGMGVQPDWQGRGIADALIEAAEAELERT
                     GCRRVNLHTTQPLARAQRFYARHGFRLTGDVEDFFGMEIAEHAKDL"
     gene            complement(2891..3409)
                     /locus_tag="VFR25_01635"
     CDS             complement(2891..3409)
                     /locus_tag="VFR25_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9939781.1"
                     /translation="MTTRLDWKDAVWGQFGAAIDSMELAIDACPDEVWGDRSRFPEYW
                     YVVYHTLFFLDYYMSESPEGYAPPAPFTLSELDPAGVLPDRVYSKEEMRRFLEHGRQK
                     VRARVAGMSDEKAASRSPFRDLGELDFLLYQMRHVQHHTAQLNLIIRQVTNSDAPRWV
                     GRTKLPLNPSGS"
     gene            complement(3406..3882)
                     /locus_tag="VFR25_01640"
     CDS             complement(3406..3882)
                     /locus_tag="VFR25_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9939782.1"
                     /translation="MDHDAALRDHVAKLILWEDAHGGFDSAVDGIAAASRGVVPDGFP
                     HSAWQLVEHLRLAQADILDFCRNPDYEEGTFPDDYWPKSAAPPYEKAWDESLAAFRKD
                     RSDVVGLVQDPKRDLFARIPHGSGQTYLREALLVADHNAYHVGQLIALRRMLGIWK"
     gene            4019..5797
                     /locus_tag="VFR25_01645"
     CDS             4019..5797
                     /locus_tag="VFR25_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET9939783.1"
                     /translation="MRRLALFATLLCVSALFLTTESGFAGPSQDLAKLCDKYWQGYLQ
                     AYPTAATGLGDRRYDDRLDDISPTGIAKERKRLEGVAAAARGISPDKLSQADRLTREA
                     LIEEVEDQLAWYGCEFEQWVVDPLGGPQVQLMNIPDYTTIETPKDAAAYVKRVRAMGP
                     YLNDHVANLRDGLSLRKTASVDAVLKTIDELTQLTSAPTESLAVWKPSQATHDGWLQA
                     DRDRFATDLRDAIESSLRPGLVRYRDMLQSEVLPAARPPEKAGLAALPGGLECYKKMI
                     RMHTSLDLDPEQIHQMGLDAVAAFRRDLSALGQKVFGTSDIVEIQRRLREDPAMHFKT
                     GAEIEAKAREALARAQAAVPQWFGRIQPKAPCEVRVMGMHEAPHSTIAYYRHPSDDGK
                     RPGTYMINTYLPATRPRYEAEALAFHESVPGHHLQIAVAQELTGIPEFRKHQGVTAFV
                     EGWGLYAERLADQMGLYTSDVDRMGMLSFDAWRACRLVVDTGLHAKGWSRQQAIDYMT
                     DNSILAENNIENEVDRYITWPGQALAYKLGQMEILKLRDEAKAKLGSRFDIKAFHDAV
                     LGNGAVALPVLRQQVQAYIATASVAN"
     gene            complement(5830..6426)
                     /locus_tag="VFR25_01650"
     CDS             complement(5830..6426)
                     /locus_tag="VFR25_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9939784.1"
                     /translation="MGKGRLEAFSDGVLAILITIMVLELKVPHGEDLASLVPLIPKLL
                     SYVLSFVYLAIYWNNHHHMLHVTHKVTGPILWANMHLLFWLSLIPFATGWMGENRFAP
                     VPTAVYGVPLLMAGIAYWILLQAILKAEGPDSVLAHAVGEDLKGKSSILLYVAALGLA
                     FVNPAIARAIYVLVALIWLIPDRRIERALAEYEARSRD"
     gene            complement(6427..6774)
                     /locus_tag="VFR25_01655"
     CDS             complement(6427..6774)
                     /locus_tag="VFR25_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding ACT domain-containing
                     protein"
                     /protein_id="HET9939785.1"
                     /translation="MGEVFGRAGVSVEGGGVFLAGGVGVAHFLFVDGHAARRALEAAG
                     IRVLAVNEVVALRLRQDEPGQLGKIARRMAEAGVNIEVQYSDHDHQLILVVDDLAAGR
                     AVADAWMRERAGA"
     gene            complement(6828..7178)
                     /locus_tag="VFR25_01660"
     CDS             complement(6828..7178)
                     /locus_tag="VFR25_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET9939786.1"
                     /translation="MSRLVEIRSYNLKPGTRAEFHRLVMERAMPLLEQVRMDVVAFGP
                     SPHDDNSYYLIRAFDDPAHRDASEAAFYGGAPWREGPREAILALIENYAEFVIELDEA
                     VIDRLRTRPVVPRS"
     gene            complement(7212..7667)
                     /locus_tag="VFR25_01665"
     CDS             complement(7212..7667)
                     /locus_tag="VFR25_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9939787.1"
                     /translation="MIRGAKYSHTNLIARNWRALAKFYAEVLGCVPVPPERDYQGADL
                     DRGTGIRGARLTGAHLRLPGRGPDGPTLEIYHYSILEHSESPRVNRPGFGHIAFEVDS
                     VMEARDEVLRAGGSPVGDIVTLTTATGAKVTWCYVTDPEGNIIELQSWA"
     gene            7809..11645
                     /locus_tag="VFR25_01670"
     CDS             7809..11645
                     /locus_tag="VFR25_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9939788.1"
                     /translation="MRASLPGYQLVPLLLVALVSAASASAAPSTLEVALSESAAQRAE
                     QVSNAVPVLAHVSDMTVNPGEVADQAITATDGDGNPLTFGKSFGPAFVTVTTTTPGTG
                     SAQGNIHAAPAVSDAGTNQVGITVSDGFATVAQSIEVEVPGGDHAPVLTQPNNMSVPA
                     GNTVTQELTAVDNDGNALSFYVNGPSFATVSTNDPGAGLASGTLRLATLTTQATGAYP
                     ITVGVTDGTLTNEKSLTATVLPRLNTAPLLFQPENAQVQGGSAVDVELHAVDAEHDVL
                     SFSLVSGPAFVTVSTVNATAGLGNAHIAPPLTVPSNTYVVTVSVSDGLASDQRSFLVF
                     ATGVHPPTLGFISNMSPRAGQTLTQSIFASDPDGGQLTFGKVSGPTYMDVATTSQSSG
                     FSQGRITLTPSTSDIGTATGVVSVTDGTLFDQRSFTITVGQPNHPPFFLTPPADMTVT
                     QGSIGEQVVQAVDADNDFVSFSIGPNSPGYMDVFSTGLIRLTPGANDVGTAQGAVRAT
                     DNFGGVANATFQITVLAGTLPPACPAGSFNVTSTPGAPSYSVRAADLNGDDVQDVVAA
                     DYDGGNTDIYFGDGAGGLAAPVHYPSFHPIDAAIGDFNGDLIPDLASLEYSPSSVSVR
                     LGLGPGTFGPLQSFLIGESPTRLLTADFDRDGHLDLVASTYSYTATLSFLYGSGTGGF
                     EPARNLALNDYCVDIQVVDVNHDGWPDILATFGSSRAAILTNLHNRNFSVTTVPVSNY
                     PYSIRLADLNGDGVLDMITNDGNNPFVRVYLGTGPGTFGPATAYSPQQYVYALEVGDF
                     NGDSLPDVATVGNLGTTILLGNGAGLLGSRMMLAGPLLYSLAVADMDNDLRADLVSGS
                     YDLAGVQTMLNRCAPQRDRPPHVLAPRSASTQEGAVVTINVTASDPDGEPITQLRADA
                     SGLPLGNDAALVPNGANTGGVFTWTPTSADARPAPYPVVFIASNAISGSTTTNVTVTN
                     VNRAPSANAGGPYNALADVAIQFQGGASSDPDGDALTYSWVFGDGTSGKGPAPMHTYH
                     ATGTYAVGLVVSDGALTGVATTTVSVLGVFPARAFTLSGNKTIRLNSGKPKWCVEIEP
                     SNTSFLLQVVDLSSIRMKSQGTGSVSEISAIADKSSIGADRDGNGITEFEACFAKTDL
                     RQLFSKVHGSAAVTVTIEGSLLTGGSFRASMDVGVQASGGGNLATSLSPNPLNPSGIL
                     TLRLERSGPLRVAIYDVTGRLVRVVCDESMATAGYHDFVIDGRGSHGETLSSGVYFYR
                     VEAAEGVDRGRFTILK"
     gene            11655..14093
                     /locus_tag="VFR25_01675"
     CDS             11655..14093
                     /locus_tag="VFR25_01675"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET9939789.1"
                     /translation="MRPRTLHHRAGLLVAGAGVLIAVTLAPSALARPPATGDAPNRAP
                     AVIDNDDRFPITNLDMQLSNHGSFARTSTQPGVIYPRGSGHGVIYAAGPWIGGVVAGS
                     LRLAIGEYNGNFAPGPMVGGGYFPDAPEFRNYTITRGNTSSADFLGWPAGQGAPVDAF
                     GNPALLGDAMTWCVYNDVGPHSIVDFAFPNPLGIEIQQSTFGFNGSGPLANVIFLRFR
                     MTNGTPTPISDAYFSLWSDPDLGEAFDDVAGCDPGLGLGYVYNADNADRDYGSTPPAT
                     GFLLLQGPILANGTAFDTLGVTAFRAYRGNEDPQGIERYSQMRGLLDDGSPMHQQNDP
                     ALPVTTYQFSGDPVQGTGWLDLLPGDKRILMSSGPFPLGPGETQEIWAAVLVGQGADR
                     LSSITALRDVAAAAKAAYRQGFLLHVEAGAPAHRSVDEGATLSFDVGGRAPFGHPVTL
                     RVAEGPPGGMFQDHGDGTGTFTWTPDFQQAGSHVARFEATSDGAVSSATTTIDVRNVN
                     RGPDARAGGPYTTFMGFPVTLDASATSDPDGDALTYHWDFGDETEGMGAAPVHTYAYR
                     GTYGVALSVSDGTLASDATTSVEVLDLLRARAFTAGGNKTIKLSSGKPAWCLELEPVG
                     QSFAISDVDLATLVMRSAGTGRVDEIPAIGGKTSTGSDRDGNMVPEISACFRTTDLAD
                     LFSKLHGTTTVNVTLNGYLFSGAPIQGLMSVGVQAGGGGQLAASVRPNPMNPTGTISW
                     RTSKAGPVRVDLYDLAGRLVRRLVDDTSQPAGWHEATFDGRDGAGRELASGVYFYSIQ
                     TIDGMERGRLAILQ"
     gene            14190..16124
                     /locus_tag="VFR25_01680"
     CDS             14190..16124
                     /locus_tag="VFR25_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9939790.1"
                     /translation="MPSFGYRHGWNQRGPKAFACCLIALAVWTAPTRAQVIAAGNLAD
                     LTLEQLSNIEVTIATRRAQPLADVPASAFVISSEDIRRSGVTSLPEALRLAPTLQVAR
                     ADANQYAISARGFNNVLANKMLVMIDGRVVYSPLFSGVFWEAQGVMLDDVDRIEVVTG
                     PGGTLWGTNAMNGVINVVTRSAEETQGALLDAGSGNHDTVIGARFGLAPGSATHVRAY
                     GQFSSRDDTERGGDLDLPDASDRLQAGFRGDWGDASTAMTLSGDLYRGNITQPSPGGD
                     TTRVVSGGNVLGSWTHNYKTGSQLRVQGYFDYSEREQPDAIHESLQTWDIQAQHGFRP
                     GEKHDAVWGVEYRYQADRVANLTPLIGFLPPDRDLRTASLFGQDVFTVHERVELTGGV
                     RFERNEFTDWEFLPNARLAWKPQDARLLWAAASRAVRAPSRIDREIFSPVTTPHLLLA
                     GGPGFESEVARVFELGYRDQPTRDLSFSLTGFHHNYDDLRSLTTSPDGPIFANGIEGT
                     NTGVEIWGSYRASTAWRFTAGWVQQSIDLDVAPGHSALAGTATLGNDPKSWGQLRSAL
                     DLGDVDVDAAVRYVGRLPDPEVPSYATLDARLGWHPTKDFELSLSGWNLTGGSHPEWG
                     VPGARPDFERSVFLKIKMGS"
     gene            16153..16701
                     /locus_tag="VFR25_01685"
     CDS             16153..16701
                     /locus_tag="VFR25_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HET9939791.1"
                     /translation="MIRWILLSSALLAILSGSARAQSDDSDLERRVKAAYLYKFAGYV
                     EWPDSSFPDPTTPVRIAVLGDDKLRAELATMVLGHTVNGRPVVVLGYTEEDSLEGVHV
                     LYVGRQETSRLPKLKDEARKRSVLLVSEGDDAIDQGSVIAFVLSRRRVRFDISMACAA
                     KSGLTLSSRLLSVARTVVTAGS"
     gene            16698..18608
                     /locus_tag="VFR25_01690"
     CDS             16698..18608
                     /locus_tag="VFR25_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9939792.1"
                     /translation="MIFGGIRRSIRRKFMLVVMATTLVALLLASIALVTYDLLTYKRT
                     WVADLSTQADILGLASAPALSFQDPKTAQENLGLLSVRPNVTAAAIYTSDGRLFASYR
                     RDSTDTTPLPPHPGPAGHEAGTREISLFRPIVTPDEQLGWVYLRARYDLTGRLLSYLA
                     IVVGVMLASLTVAALMSVALQASVIRPILAIADVAHEVKDRRNYAARVPKTTEDETGY
                     LVETFNDMLGEVGQRTEDLERANRRLEQEVAVRQNAEHALIEADRRKDEFLATLAHEF
                     RNPLAPLRNALHILKVAGPGSEDGKVARDIMDRQLRQLVRLVDDLLDVSRITTGKVVL
                     QRESVPLATIVESALETVRPFVAERGIALSVTMPDLPLMLRADPTRMAQALTNLLHNA
                     AKFTDAGGRIDLRVEREGLEGITITVADTGIGIPADMLPFIFDMFTQLDRSLERPYSG
                     LGVGLSLAKRLIELHGGSLEAASEGQGKGTRFTARLPGIVVFEPGPMAASNDGGAPAA
                     PARRRVLLADDNIDFTSSFALMLRSMGHDVIEAHDGAEALQAARAIQPEVAFLDIGLP
                     KMNGYDLARRLREDPATRRTVLVAVTGWGQDGDRRLAAEAGFDLHLVKPVESAQIETI
                     FAGLASTPLRPA"
     gene            complement(18632..20017)
                     /locus_tag="VFR25_01695"
     CDS             complement(18632..20017)
                     /locus_tag="VFR25_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HET9939793.1"
                     /translation="MRRGGGSPVRTALIVAVVAAIAAGGIAALLINIFQRQAEGRNPF
                     YRVVEINETTEDPAVWGKNFPAEYDGYKRTVDMVRTRYGGSEAEERTPTAADPRSVVA
                     RSRLEQDPRLKTMWAGYAFAVDFREERGHAYMLDDQTFTERQLVTTQPGTCLHCHASI
                     VVPYRKLGDGDLARGFERMNAMSYADARKLVTHPIACIDCHNPQTMELRVTRPGFMEG
                     IRALKASQGIQNYDVNKDATRQEMRSYVCGQCHVEYYFKGKEKRLVYPWAKGIKIEEI
                     QAYYDEIQQKDWIHADTGAPVLKAQHPEFEMWSQGIHARSGVACADCHMPYMREGALK
                     ISDHHVQSPLLNINRACQTCHKFPEAELRQRVEIIQDRTFKLRNQAMDALVDLIEDLK
                     HAQAVGQNEVRLEAGRRYQRLAQFRLDFVEAENSNGFHAPQEAARILGESIDYSRKGQ
                     IVLRDGTKGRN"
     gene            complement(20036..20458)
                     /gene="nrfH"
                     /locus_tag="VFR25_01700"
     CDS             complement(20036..20458)
                     /gene="nrfH"
                     /locus_tag="VFR25_01700"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HET9939794.1"
                     /translation="MLAGLAVGVAVGTGLYTFTYAHGGAYLTNDPRACANCHIMREQY
                     DGWVRSTHHATATCNDCHTPHDPIGKVTTKARNGFWHSLHFTLGDFPEPIQIKPHNRA
                     IVEKNCRSCHGPIVAMMTAGHPSSDPSCIRCHGSVGHP"
     gene            complement(20802..20951)
                     /locus_tag="VFR25_01705"
     CDS             complement(20802..20951)
                     /locus_tag="VFR25_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET9939795.1"
                     /translation="MLVTIAVLLVVLWALGLITKVAVGGFIHLLLVAAIVVFLLSVIS
                     GRRAV"
     gene            complement(21076..21753)
                     /locus_tag="VFR25_01710"
     CDS             complement(21076..21753)
                     /locus_tag="VFR25_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9939796.1"
                     /translation="MVRSWAIALWVLVLTVAIAVVAGMLRHEPVVKRAEALTAAANPS
                     IQLSEILAGPSRDWDGDGAFVARQDEWLEIRNNGASVETLAGYLVSDADSTIRYAFSG
                     SLAPNAVILVTGRMAEDWQRAQSRTVSGLSLNNSGDTVRLFQIQGSDTVEVESKTYNS
                     IEGATDRSTGHLGPSDAWVLFDGLNRYAGSGTPAGTGCNPTPGAVNDCPTATEQTTWG
                     KIKMRFR"
     gene            complement(21934..22980)
                     /locus_tag="VFR25_01715"
     CDS             complement(21934..22980)
                     /locus_tag="VFR25_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939797.1"
                     /translation="MIPGRSRGGPWVLLGNAALLVALFTGSYLIAAAAGSSQGWPPWS
                     GDIVLAAAGLLFLGLAFLFPLHAGLLNFGIHAQFLAGFIAGSLIARSADILPGAQGLL
                     GLIAGAGGGLFVGLVMAWLKRKLAIHEILSGLLLAAMLIPVARAFGVEPAAAPALEIQ
                     LGTLARTLAWAPGLHLAPSFVMAWGILLLALGIGLGLLAAHLLRASVRGFELRAVGSN
                     PLAAVAAGVDVDAMQTLTIAAGGACAGLVGALQLWTDPAVALERWPVPLGFAGITVAF
                     LGGGYLRGALVAAIVLAVWLNTPGVAAALSSPLWGGAMALLLVLPAFWNLPRLTPDQG
                     APRAIWRTRHRESV"
     gene            complement(22977..24455)
                     /locus_tag="VFR25_01720"
     CDS             complement(22977..24455)
                     /locus_tag="VFR25_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9939798.1"
                     /translation="MVDKNVRLDVKGMALADGPRWLVQDLSFSVGPGSVHMLLGERDT
                     GKTAILEALAGRRKLERGTLLAPAPAALVPQPAGSAQIRVLDRLILHRQPKRMGMFIH
                     WKQAREEARALAARFALEGLLDVPVETLRPLERRLLELAASLADQPALLLLDEPTSEL
                     GPHEARHFLATIRRVASEEKIPAVITSAFPRDAYPEAEAVTILWRGAEPVTVTTHECS
                     EGTLVERWTGGLGIRRTPSGHHTPGEPLLRIEDLVLRGRGRETSLAGLEFEVKAGEVL
                     AIVGAPADGLNLLHDVLLGSRSPEQGSMQFLGKEMILSTRRHRVESGISFVNPPHARD
                     QAIPEFTIEENLILGQTTGPPFARKGFLRHDSIRGNAVRTLRDFEIPEAEAHHRFRQL
                     KLGDRQRVIVAREVLRNPVLMVARSPAQGLSLAAQEYVRRALVLQCERGSGLLWLTEE
                     PEEAMRVADRLAVLAAGRFVWIPVTETLTREMIIDEMSGAAA"
     gene            complement(24538..25491)
                     /gene="arcC"
                     /locus_tag="VFR25_01725"
     CDS             complement(24538..25491)
                     /gene="arcC"
                     /locus_tag="VFR25_01725"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET9939799.1"
                     /translation="MDRLPHSILITLGGNAILPARGTGTFDEQCAITRLTMQPIAALI
                     QQGVRVVLSHGNGPIVGNILIRNEAARDQIPPMPLDVCGADSQGGIGYMMQQALDNEL
                     RRIGIQRPVTTLVTQVIVDERDPAFRRPTKPIGPFYTQERARLLAKEKGWTVTEDAGR
                     GWRRVVASPKPLEVVEITAIRKLVEDGAIVIAAGGGGIPVARQWDGTLHGVEAVIDKD
                     LASSLLARLLGCEMLCIITGVDRVALHYGKPEQRELPCATAEELSEYAAAGHFPAGSM
                     GPKVQAAIEFVRGGGREVVITSPAKLTEALEGTTGTRVRRE"
     gene            complement(25492..26712)
                     /locus_tag="VFR25_01730"
     CDS             complement(25492..26712)
                     /locus_tag="VFR25_01730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9939800.1"
                     /translation="MRTPRFGRTDEEKSMRYADRMSQLGTETAFEVLAKARALEAKGR
                     TIVHLEIGEPDFDTPASIRQAAIRAIEEGFTHYGPSAGLPEVREAIAQYITKDRGLPV
                     APEQVVVTPGGKPVLTFAVLACVNPGDEVIVPNPGFPIYESAVRFAGGTPVSLALRED
                     RSFRFGAADVEKLLTPRTRMLVLNSPHNPTGSVLTPKDLDEIASLLRDRDVFVLSDEI
                     YSKILYEGTHETIATRPGMAERTIILDGFSKTYAMTGWRLGYGVMNPTLAKHVARLAT
                     NVYSCATSFVQRAAPGALLGPQDEVRAMVEEFRRRREAIVEGLNAIPGIKCAKPSGAF
                     YVFPNITGLGMKSSQVETALLEEAGVAALSGTAFGSNGEGYVRFSYANSIENIREGLK
                     RFEAFAQAHANVTR"
     gene            complement(26719..>27233)
                     /locus_tag="VFR25_01735"
     CDS             complement(26719..>27233)
                     /locus_tag="VFR25_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939801.1"
                     /translation="EGRIAPAGYGPLSFRIGSARTESFSNAAGFHASIERYAVAEMQV
                     ARADGRTLTLLASRRDKETVAGVRRGSSLGGRLSLAWRERATLEIQLEGIRAVRDGAA
                     WSSGLYASGATALLTTARPGVAATARGAWKLGRWTLGGLVEQRDDVSGERATAASIWV
                     GRELRESGRE"
BASE COUNT         4469 a         9418 c         8917 g         4429 t
ORIGIN      
        1 cttgtccgga atggcgaagc gacggagcgt caccgagggg gcgaacggat tgacgcggac
       61 atccccgagc gtgggcgtcg tggcgagact cgcgcccagg ttcttcagca ggaccgagcg
      121 gaggattcgc ggcgcgacca gaaaaccggc caccgcgtac agcgcgaggc cggcgcctac
      181 gaagagagtg atcttcttca gacgcggggt cattgggctc ggtcaccgga gtcgaggaac
      241 gaggggattc tctgcgaacc tagccccggg actccgctcg gtcaagcccg gttcgatcgc
      301 ggggcacccg caatcctacg cacggtcccg ctgcactcct acgtgcggtc cgcccgcacc
      361 gccgagccaa gcgagagcat cttcctcgtt ccggaagacc tccgcctcca ctcccaccga
      421 ttccgaatag acgcttccca tgcggctcaa gccgaagtga acgtcctcgg tcgcgacgac
      481 ggcgacccgg cctccgatcc gctcggagta aggcgcgagg gcctgggcca cgtaccggat
      541 ctgattgggg cttcgtgttc caagaacctc cgaccgggtg gtatccacga gcagcgcgat
      601 cttctccgag ggtggcgcct ccgcgagcgc cgtctcgaac tggcggatga tgtcgtcggg
      661 ctcgtacgtg cccacgaaac tcatccggag gagcgtaccc tcccaagcgt gaatcactgg
      721 catgaggctc cccctttccg tcccataggc tactttccct cgatctggaa gcgcaccaat
      781 ccgctcgcct tgtcgagggt caccttcacc gaccacatgc cgggcttccc cttgagcgac
      841 accatcgtcc cctgttcact cagattcttg gaatggacga cggatccgtc cgcgtcgcga
      901 acctcgacga gcgcctcacc cccctggatg tcgctcccct gccgtaccac cgcggaggag
      961 tcgggattcc accatgcgta gttctggctc atggtgaagg tcttcgcggt tccgatcttg
     1021 aactcgaagt agtcggagcg attggtcacc tggaagtcgt tgcccgatgt gtgcgtcgaa
     1081 gcacagcccg cggcgagcag gagccctgcc gcccaggccg cggccgagag ccgcttcacg
     1141 cgccgcctcc cttcacagcg gactcacgct ccatgatctg ttccaccacg tactccgcat
     1201 cttcccccac gccgaggaac agcgccgact tcctcttgtg aagccagtgc atgcccacga
     1261 agtagagccc aggcaacgtc gagacgccgc gccggtgcac cggccaaccc tgctggtcca
     1321 gctccagccc ttcgatccaa cgatactccg gtcggaaacc attggcccag atcatcgtgc
     1381 tcacgcccgc ctgacgcaga tccagctcgc ctggtgagga gatggggtcg cgagggtctt
     1441 ccggttccgg atcggcggtg tggataccct tggctcgaac gtagtcgtcg acgcgctttc
     1501 gataggccgc gacgaactcg tcccccatcg tcagcgattc ctcgagccct cccccgatct
     1561 tgagcttcca cccgtccacg ccttccaggc gcccgagcag aaccgctccg cgacgcgcga
     1621 gccagcgcgg attgcagtcg tgaccgccgt cgttgcccga taccggcacg ttgcagagca
     1681 gccgggccgc aggcgaagga agcgtatcca ccgtttggtc cgcaagcccg gtctcgagca
     1741 tccagtgcat cagctcgcct ccgcgatatc gtcgcggcag ccacggacag cgtcccgccg
     1801 ccaggtagac ctggcgcccc gcgtcgatca gctcgtccgc gatctggcac ccggactgac
     1861 cgctccccac catcacgacc gccccgtcgg gaacctggct cgggcggcga tactggctcg
     1921 tgtggatctg catgacgtca gatggaaccg cggccatgag cggcgtgggc gtggggcgct
     1981 gatacgcacc cgcggccacc acgacgttgt ccgcggtgag cgctcccccg ttcgtctcga
     2041 ccgtgaagcc ggagccatcc ggccgcaccg ccaggacctc gacgccttcg cgcaccggag
     2101 cgcggaagct ccgcgcgtag tcttcgagat acgagatcac ctcgggaagc gcggcgaacg
     2161 cgtccgggtc gggtcccgcg taggcaaacc ccggcagctt gtgacaccag ttcggcgtgt
     2221 tgagatagaa gccgtcccag cgctcggagc gccacgtctc cgcgacccgg ccgcgctcca
     2281 gcacgagatg ctcgcgccca tgggtgctga ggagatagct cgtcgagagg ccggcctgtc
     2341 ccgcgccgat gacgatcgtc tcagaggtcc ttggcatgct cggcgatctc catcccgaaa
     2401 aaatcctcga cgtcacccgt gagccgaaag ccatggcgcg catagaatcg ctgggcgcgc
     2461 gccagaggct gagtcgtgtg cagattcacg cggcggcatc ccgtgcgctc gagctcggct
     2521 tcggcagcct cgatcaaggc gtccgcgatc ccgcgtccct gccagtctgg ctgcaccccc
     2581 atcccgcgaa ggtggcctag cccgcccccc acgtcctggc atgcgatcgt gcccacgatg
     2641 gccccatccg gcgccgtcgc gacgaagagc gccatgtcgc gaagcctttc ttcgatcccc
     2701 gcgggcgatg gcaccgtgtc gcggaacgct ccgtgggtgt actgcgctcg gtagggctcg
     2761 aacgcggccg tgaggcagag atggatcgcc aacgcatccg tcttcacggc ccggcggacc
     2821 tcgaactcag gcgtcgcact cggctcggca ggagtcatta gcccgtcagc ctaccatgtg
     2881 cagaggcggc tcaggacccc gacggattga gcggcagctt ggtgcgcccg acccagcgtg
     2941 gagcgtccga gttggtcacc tggcggatga tgaggttgag ctgcgccgtg tgatgttgca
     3001 cgtggcgcat ctggtagagg aggaagtcga gctccccgag atcgcgaaac ggggagcgcg
     3061 aagccgcttt ctcgtcgctc atgccggcaa ctctcgcacg caccttctgg cggccgtgct
     3121 ccaggaagcg ccgcatctct tccttcgagt agacgcgatc cgggagcacg cccgcgggat
     3181 ccagctcgct cagcgtgaag ggagccggcg gcgcgtagcc ttccgggctc tcggacatgt
     3241 aatagtcgag gaagaagagc gtgtgataga cgacgtacca gtattccgga aaccgcgaac
     3301 gatcgcccca cacctcgtcc gggcaagcgt cgatcgcgag ctccatcgag tcgatggcgg
     3361 ccccgaactg cccccacacc gcgtccttcc agtcgagtcg ggtcgtcact tccagatccc
     3421 gagcatgcgc cgcaacgcaa tgagctggcc gacgtggtag gcgttgtggt ccgctacgag
     3481 gagcgcctcg cggaggtagg tctgtccgga gccgtgcggg atccgggcga agagatcgcg
     3541 cttcggatcc tgcaccagcc ccaccacgtc gctccggtcc ttgcggaaag cggccaggct
     3601 ctcgtcccac gccttctcgt acggcggggc cgcgcttttc ggccagtagt cgtcggggaa
     3661 ggtgccttcc tcgtaatcgg ggttgcggca gaaatcgaga atgtccgcct gcgcgaggcg
     3721 caggtgctcg acgagctgcc acgcggagtg cggaaatcca tccggaacga cgccccgcga
     3781 tgcggcggcg atgccgtcga cggccgagtc gaagccgccg tgcgcgtctt cccagagaat
     3841 gagcttcgcc acgtgatcgc gaagcgccgc gtcgtgatcc atacctcact ctagcctcgc
     3901 atccggcgcc aggtcaacgc gcccgcggcc gccacaaaac gaagcctccg ttgccgcgaa
     3961 aaggggctat cattcggccg cgccgtcgcc caccccgccg tgccggaggt caattcccat
     4021 gcgtcgcctt gccctgttcg ctaccctgct ttgcgtgtcc gcactgttcc tcaccacgga
     4081 gtcgggcttc gccgggcctt cccaggacct cgccaagctc tgcgacaagt actggcaggg
     4141 ttacctccag gcctacccga cggccgccac gggcctgggt gacaggcgtt acgacgaccg
     4201 gctcgacgac atcagcccca cagggatcgc gaaggagcgg aagcgcctcg agggcgtcgc
     4261 cgcggccgcg cgcggcatca gtccggacaa gctgtcccag gcggatcgcc tgactcgtga
     4321 agcgctgatc gaggaggtcg aagaccagct cgcgtggtac ggatgcgagt tcgagcagtg
     4381 ggtggtcgat cccctcggcg ggccccaggt ccagctcatg aacattccgg actacacgac
     4441 catcgaaacg cccaaggacg cggccgcgta cgtgaagcgc gtccgcgcga tgggacccta
     4501 tctcaacgat cacgtggcca acctccgcga cggcctctcg ctccgaaaga ccgcttcggt
     4561 cgacgccgtg ctgaagacga tcgatgagct gacgcaactc acctccgcgc ccacggagag
     4621 tctcgctgtg tggaagccgt cgcaggcgac gcacgatgga tggcttcagg ccgatcgcga
     4681 ccgcttcgcg accgacctgc gcgacgccat cgagtcctcg cttcggccgg ggctcgtgcg
     4741 gtatcgcgac atgctccaga gcgaggtgct cccggccgcg cgtcctcccg agaaggcagg
     4801 actcgcggcg cttccgggcg gcctcgagtg ctacaagaaa atgatccgca tgcacacgtc
     4861 gctcgatctc gatcccgagc agatccacca gatgggcctg gacgccgtcg ccgccttccg
     4921 tcgcgacctc tccgcgctcg gccagaaggt gttcggtacc tccgacatcg tcgagatcca
     4981 gcgccgtctg cgcgaggacc cggcgatgca cttcaagacc ggcgcggaga tcgaggccaa
     5041 ggcgcgggaa gcgctcgcgc gcgcacaggc ggcggtgccg cagtggttcg ggagaattca
     5101 gcccaaggcg ccgtgcgaag tgcgcgtgat gggaatgcac gaggcgcctc actcgaccat
     5161 cgcgtactac cggcatccct ccgatgacgg caagcgcccc ggaacctaca tgatcaacac
     5221 ctaccttccg gcaacgcggc cccggtacga agcggaggcg cttgcgttcc acgagtcggt
     5281 gcccggacat cacctccaga tcgcggtcgc gcaggagctg acggggatcc ccgagttccg
     5341 gaagcaccag ggagtcacgg cgttcgtcga aggctgggga ctctacgcgg aacggctcgc
     5401 ggaccagatg ggcctctaca cgagcgatgt ggatcggatg gggatgctct cgttcgacgc
     5461 ttggcgcgcg tgccgcctcg tcgtggacac ggggctccac gccaagggct ggagccgcca
     5521 gcaggcgatc gactacatga cggacaacag catcctcgcc gagaacaaca tcgagaacga
     5581 ggtcgaccgc tacatcacgt ggcccggcca ggcgctcgcc tacaagctcg gccagatgga
     5641 gatcttgaag ctgcgcgacg aggcgaaggc gaagctcggg agccgcttcg acatcaaggc
     5701 attccacgac gccgtgctcg ggaacggagc ggtggcgctt cccgtgttga ggcagcaggt
     5761 ccaggcgtac atcgcgacgg cttcggtagc gaactagcgt ccaccgaccg cgccattcgc
     5821 tggaagcgat cagtcccggc ttcgggcctc gtactcggcc agtgcgcgct cgatccgccg
     5881 gtccgggatc agccagatga gcgcgaccag cacatagatg gcgcgcgcga tcgcgggatt
     5941 cacgaaggcg aggccgagcg ccgccacgta gagcaggatc gagctcttcc ccttcagatc
     6001 ctcacccacc gcgtgggcca gcacggagtc cggtccttcc gctttcagga ttgcctgcag
     6061 gaggatccag tacgcgatcc cggccatcag gagcggcacg ccgtagaccg ctgtcggcac
     6121 cggcgcgaat cggttctcac ccatccatcc cgtcgcgaat gggatgagcg agagccagaa
     6181 gagcaggtgc atgttggccc agaggatggg cccggtcacc ttgtgcgtga cgtggagcat
     6241 gtggtggtgg ttgttccagt agatggcgag atagacgaag ctgagcacgt agctcaagag
     6301 cttggggatc aaggggacga gcgacgcgag gtcctcgccg tgcggcacct tgagctcgag
     6361 caccatgatc gtgatgagga tcgcgagcac gccgtcgctg aaggcctcga gccgtccctt
     6421 tcccatctag gcccccgctc gctcccgcat ccaggcgtcc gccacggcgc gccccgccgc
     6481 gagatcgtcc acgacgagga tgagctggtg gtcgtggtcg ctgtactgga cctcgatgtt
     6541 cacgccggct tcggccatcc gccgcgcgat cttcccgagc tgtccgggct cgtcctggcg
     6601 taggcggagc gcgaccacct cgttcacggc gagcacgcgg atgcccgccg cttcgagcgc
     6661 gcgccgcgcg gcgtggccgt cgacgaagag gaagtgcgcc acgcccactc cgcccgccag
     6721 gaacaccccg cctccctcca cgctcacgcc cgcgcggcca aacacctccc ccatggcggc
     6781 gagcgcgccg ggacgatgtt ccagctcgat cgtgacgtcg tgcagggtca gctccggggg
     6841 accacggggc gggtgcgaag acgatcgatc accgcctcgt ccagctcgat cacgaactca
     6901 gcgtagtttt cgatgagcgc aaggatggct tcgcgcggac cctcgcgcca cggcgcaccg
     6961 ccatagaacg cggcctcgct cgcgtcgcgg tgggccggat cgtcgaacgc gcggatcagg
     7021 tagtaggagt tgtcgtcatg aggcgagggt ccgaaggcga cgacgtccat ccgaacctgc
     7081 tcgagaagcg gcatcgcacg ctccatgacg agccggtgga actcggcgcg cgtgccgggc
     7141 ttgaggttgt aggagcgaat ctcgacgagg cggctcatgg acgccacttt acgcatccgg
     7201 aggcgtcgag actaggccca tgactggagc tcgatgatgt tgccctcggg atcggtgaca
     7261 tagcaccagg tcaccttcgc gccggtcgct gtcgtgagcg tgacgatgtc tcccaccggc
     7321 gagcctccag cccgcagtac ctcgtcgcgc gcttccatca ccgagtcgac ctcgaacgcg
     7381 atgtggccga atcccggccg gttcacgcga ggcgattccg agtgctcgag gatcgagtag
     7441 tggtagatct cgagcgtcgg accgtccggt ccacggccgg gaagccggag gtgggccccg
     7501 gtcagcctcg ctccgcgaat gccggtccca cgatcgaggt cggcgccctg gtagtcccgc
     7561 tcgggcggca ccggaacgca gccgaggacc tcggcgtaga acttcgcaag ggcgcgccaa
     7621 tttcgcgcga tcagattggt gtggctgtac ttggctccgc gaatcatgga ccgtcgcacc
     7681 tccccgtcaa cttcttcctg ttcgcttatg gcgccccgaa cgttctagct ctgcgggggg
     7741 tacagcgcca ttcgagtcac cctccccacc ccagactacc cgagcagcga tcacaggagg
     7801 gccacctcgt gcgcgccagt ctccccgggt accagctcgt cccgctcctt ctcgtagccc
     7861 tcgtctccgc cgcttccgcc agcgccgccc cctccacctt ggaggtcgcg ctgtccgaga
     7921 gcgcggccca gcgtgcggag caggtcagca acgcggttcc ggtcctggct cacgtcagcg
     7981 acatgaccgt gaaccccggc gaggtcgcgg atcaggcgat cacggcgacc gacggggacg
     8041 gaaacccgct cacgttcggc aagtcgttcg ggcccgcgtt cgtcacggtg accacgacga
     8101 cgccgggcac aggctccgcg cagggaaaca tccacgcggc gccggccgtg tcggacgccg
     8161 gcacgaacca ggtcggcatc acggtgagcg acggattcgc gacggtggct cagtcgatcg
     8221 aggtggaggt gcccggcggc gaccacgcgc ccgtattgac ccagcccaac aacatgtcgg
     8281 tcccggcagg aaacaccgtg actcaggagc tgactgcggt ggataacgac gggaacgcgc
     8341 tgtcgttcta tgtcaacggt ccatccttcg cgacggtctc gaccaacgat cctggagccg
     8401 gtttggcatc gggaaccctg cggctggcca cgttgacgac ccaggcaaca ggcgcctacc
     8461 cgataaccgt cggtgtgacc gatggaacgc tgaccaacga aaaatcgctc acggccaccg
     8521 tcctaccccg actcaacacc gccccccttc tcttccaacc tgagaacgct caggtccagg
     8581 gaggcagcgc cgtcgatgtg gagctccacg ccgtggacgc ggagcacgat gtcctctcgt
     8641 tctcgctcgt ctcagggccg gcattcgtca ccgtgtccac ggtcaacgcg accgcgggcc
     8701 tgggaaacgc gcatatcgct cccccgttga cagttcccag caacacgtac gtggtgacgg
     8761 tttcggtttc ggacggcctc gcgagcgacc agaggagctt cctcgtcttt gcgaccggcg
     8821 tccacccccc cacgctggga ttcatcagca acatgagccc ccgtgcgggc cagacgctga
     8881 cgcaaagcat tttcgccagc gatccggacg ggggccagct caccttcggc aaggtgtccg
     8941 gccccaccta catggatgtc gcgaccacca gccagtcctc cggcttcagc caagggagga
     9001 tcacgctcac gccttccacc tcggatatcg gcacagccac aggcgtggtg tcggtcaccg
     9061 acggaacgtt gttcgatcag aggagcttca ccatcaccgt cgggcagccg aatcatccac
     9121 cgttcttcct tacgccaccg gcggacatga ccgtgaccca aggctccatt ggagagcagg
     9181 tcgtccaagc cgtcgatgcc gacaatgact tcgtcagctt ctcgattggt ccgaactcac
     9241 ccggctacat ggacgtgttc tcgacagggc tcatccgatt gacgcctggc gcgaacgatg
     9301 taggaaccgc gcagggagcc gtgagggcca ccgataactt cggaggcgtc gcgaacgcga
     9361 cgttccagat cacggtcttg gccgggacgc tccctcccgc gtgccccgcc ggctccttca
     9421 atgtgacgtc gacgccaggc gcaccgtcgt actcggtacg cgccgcggat ttgaacgggg
     9481 atgatgtcca ggatgtcgtg gccgcggatt acgatggcgg gaacaccgat atctacttcg
     9541 gagacggagc cggtggactt gcggcgcccg ttcattaccc atcgtttcat ccaatcgacg
     9601 cggcgattgg cgatttcaac ggcgatctga tcccggatct cgcctccctg gagtactcgc
     9661 cttcctccgt gagcgtccgg ctcggcctgg gcccaggcac gttcggcccg ctccagagtt
     9721 tcctgatcgg cgagtcgccc acccgcctgc tcaccgccga cttcgaccgg gatggacatc
     9781 tggaccttgt cgcctcgacc tacagctaca cggccacatt gtctttcctc tacggaagcg
     9841 gaaccggcgg tttcgagccg gctcgcaacc tcgccctgaa cgactactgc gtcgacatcc
     9901 aggtcgtgga cgtgaaccat gacggctggc ccgacatcct ggcgacattc ggctcgagcc
     9961 gggccgcgat cctcaccaat ctccacaatc gaaacttcag cgtgacgacc gttccggtct
    10021 cgaactaccc ctatagcatc cggctcgcgg atttgaacgg ggacggtgtc ctcgacatga
    10081 tcacgaacga cggcaacaac ccgttcgttc gagtctacct ggggacgggc ccggggacct
    10141 tcggccccgc cacggcctac tcgccgcagc aatacgtgta cgcgctcgag gtcggggact
    10201 tcaatgggga ctcgttgccc gatgtcgcga ccgtgggcaa cttgggcacg accatcctcc
    10261 tcggtaacgg agcggggttg ctcggatcca gaatgatgct ggccggcccc ctcctctact
    10321 cattggcggt ggccgacatg gacaacgacc tccgcgccga tctggtgagt ggcagctacg
    10381 acctcgccgg ggtccagacg atgctcaatc gctgtgcgcc gcaacgtgac cgtccgcctc
    10441 acgtcctcgc accgcggagc gcctcgacgc aggagggcgc cgtcgtgacg atcaacgtga
    10501 ccgcgagcga ccccgacggg gaaccgatca cgcagcttcg agccgacgcg tcgggacttc
    10561 cgctcggaaa cgatgccgcg ctcgtgccca acggagcaaa cacggggggc gtgttcacct
    10621 ggacgccgac ctccgcggac gcccggccgg ctccctaccc ggtcgtcttc atcgcatcga
    10681 acgcgatttc gggctcgacg acgaccaacg tgacggtcac gaacgtgaac cgggcgccct
    10741 cagccaatgc gggcggccca tacaacgcgc tcgcagacgt ggcgattcag ttccaggggg
    10801 gcgcgtcctc ggacccggac ggcgacgcgc tgacgtattc ctgggtgttc ggcgatggca
    10861 cgagcggcaa aggtccggcg ccgatgcaca cgtatcacgc gacgggaacg tacgcggtcg
    10921 gactcgtcgt gtcggacggc gcgctcaccg gcgtggccac gacgaccgtg agcgtcctcg
    10981 gggtcttccc cgcgcgagcg ttcacgctga gcggcaacaa gacgatccgc ctgaactcag
    11041 gcaaaccgaa atggtgcgtc gagatcgagc cttcgaacac gtccttcctg ctccaggtcg
    11101 tggatctctc ttcgatccgg atgaagtccc aggggacggg atccgtaagc gagatcagcg
    11161 ccatcgcgga caagagctcg atcggagccg acagggatgg caacggcatc acggagttcg
    11221 aggcctgctt cgcgaagacc gacctgcggc agctcttctc caaggtccac ggcagcgcgg
    11281 ccgtcaccgt cacgatcgaa gggtcgctcc tgacgggggg ttccttccgc gcctcgatgg
    11341 atgtcggcgt ccaggcgagc ggcggcggca atctggccac gagcctctcc ccgaacccgc
    11401 tcaatccgtc gggcatcctg acgcttcgtc tcgagcgctc gggcccgcta cgcgtggcga
    11461 tctatgacgt caccgggcgg ctggtgcggg tggtctgtga cgaatcgatg gccacagccg
    11521 gataccacga ctttgtcatc gatggccgcg gatcccacgg cgagacgctc tcgtcgggcg
    11581 tgtacttcta tcgcgtggaa gcggccgaag gggtcgatcg cggccggttc accattctca
    11641 agtaggggga cgcgatgcga cctaggaccc ttcaccaccg tgccggactc ctcgtcgccg
    11701 gcgcgggagt gctcatcgcg gtcacgcttg ccccgagcgc gctcgcgcga ccgcccgcga
    11761 cgggcgacgc tccaaaccgc gcccccgcgg tcatcgacaa cgatgaccgc ttcccgatca
    11821 cgaatctcga catgcaactg agcaaccacg gcagcttcgc ccgcaccagc actcagcccg
    11881 gtgtgatcta cccgaggggg agcgggcacg gcgtcatcta cgctgcgggg ccatggatcg
    11941 gaggagtggt cgccggaagt cttcgcctgg cgatcggaga atacaacgga aacttcgcgc
    12001 ccggaccgat ggtaggcggc ggctatttcc cggacgcccc cgagttccgc aactacacga
    12061 tcacgcgcgg caatacatcg agcgccgatt tcctgggttg gccggcaggg caaggcgcgc
    12121 cggttgacgc gttcggcaat ccagcgctcc tgggcgacgc catgacctgg tgcgtgtaca
    12181 acgacgtcgg gccacacagc attgtcgatt tcgccttccc gaaccccttg ggtatcgaga
    12241 tccagcaatc gacgttcggc ttcaacggtt cgggcccgct cgcaaatgtg atcttcctcc
    12301 gcttccggat gaccaatgga accccaacgc cgatttccga cgcctatttc tccctctggt
    12361 cggatccgga tctgggagaa gcgttcgacg acgtggccgg ctgcgaccct gggctcgggc
    12421 tcggctacgt ctacaatgcg gacaacgcgg atcgagacta cggcagcaca ccccccgcga
    12481 ccggctttct cctgctgcaa ggcccgatcc tcgcgaatgg caccgcgttc gacacgctcg
    12541 gagtgacggc gtttcgagcc taccgaggca acgaggaccc gcaggggatc gagcgttatt
    12601 cgcagatgcg cggcctcctg gacgacggat cgcccatgca ccagcagaac gatcccgcgc
    12661 tgcccgtcac gacgtatcag ttttcagggg atccggtgca aggaaccggc tggctcgacc
    12721 tgctgccggg ggacaagcga atcttgatgt cgtccggccc cttcccgctc ggtcccggcg
    12781 agacgcagga gatctgggcc gcggtgctgg tcggccaggg cgcggaccgc ctttcgtcga
    12841 tcacggctct ccgggacgtc gcggccgccg cgaaggccgc gtaccgacaa ggattcctcc
    12901 tccacgtcga agccggcgcg ccggcacacc gatccgtcga cgagggagcc acgctcagct
    12961 tcgacgtggg cggtcgcgct ccgttcggtc accctgtgac gcttcgcgtc gcggaagggc
    13021 ccccgggcgg gatgttccag gatcacggcg acggcacggg cacgttcacg tggactcccg
    13081 acttccagca ggctggctcg cacgtcgcgc gcttcgaggc gacgtcggat ggcgcggtga
    13141 gctcggccac gacgacgatc gatgtgcgga acgtgaatcg cgggccggat gcgcgggcgg
    13201 gcggtccgta caccacgttc atgggattcc cggtgacgct cgacgcaagc gccacctcgg
    13261 atccggacgg tgacgcactc acctatcact gggacttcgg tgacgagacg gaaggaatgg
    13321 gcgccgcccc ggtacacacg tacgcctacc gaggcacgta cggcgtggcg ctcagcgtct
    13381 cggacggaac gctggcgtcc gacgccacga cgtcggtgga ggtcctcgac ctcctgcgcg
    13441 cgcgcgcttt caccgccggt ggaaacaaga cgatcaagct ctcgagcggc aagccggcgt
    13501 ggtgcctcga gctcgagccg gtcgggcaat ccttcgcgat ctccgacgtg gacctcgcca
    13561 cgctcgtgat gcgctccgcc ggtaccggga gagtcgacga gatcccggcc atcggcggga
    13621 agacctcgac cggatccgat cgcgacggaa acatggtccc cgagatatcg gcctgcttcc
    13681 gcacgaccga tctcgccgac ctcttctcga agctccatgg aacgacgacg gtgaacgtca
    13741 cgctcaacgg ctatctcttc agcggggcgc cgatccaggg cctcatgagc gtcggcgttc
    13801 aggcgggggg cggaggacag ctggccgcgt ccgtgcggcc caaccccatg aatccaaccg
    13861 gcacgatctc ctggcgcacg tccaaggctg ggccggtgcg cgtcgacctc tacgacctgg
    13921 ccggccggct cgtgcggcgc ctcgtggacg acacctcgca acccgcgggc tggcacgagg
    13981 ctacattcga cggccgggac ggagccggcc gggagctcgc aagcggcgtc tacttctact
    14041 cgatccagac catcgacggg atggagcgcg gccgcctggc gatcctccag tagcttcccc
    14101 tcatcccctc acgtgctagg ctcatggggc cgccttcggg cggccccatt attttggact
    14161 ccgacctagg acttgaagag ggacccccca tgccgtcctt cggataccgc cacggctgga
    14221 accagcgtgg ccccaaggcc ttcgcctgct gtctgatcgc cctcgccgtg tggaccgcgc
    14281 ccactcgagc ccaggtgatc gcggccggaa atctggccga cctcacgctc gagcagctca
    14341 gcaatatcga agtcaccatc gcgacccgcc gcgcccagcc cctggcggat gttcctgcct
    14401 ccgcgttcgt catctcgtcg gaggacatcc gccgctccgg tgtcacgagc ttgccggaag
    14461 ctctgcggct cgctccgacg ctccaggtgg cgcgggcgga cgcgaaccag tacgcaatca
    14521 gtgcgcgtgg gttcaacaac gtcctggcca acaagatgct cgtgatgatc gacgggcggg
    14581 tcgtctattc acccctcttt tcgggcgtct tctgggaagc ccaaggggtg atgctcgacg
    14641 atgtcgaccg gatcgaggtg gtgaccgggc cgggcggcac gctctggggc acgaacgcca
    14701 tgaatggcgt gatcaacgtg gtgacccgct cggccgaaga gacgcaaggg gcgctcctcg
    14761 acgccggctc ggggaaccat gacacggtca tcggcgcccg attcggcctc gcacctggat
    14821 cggccacgca tgttcgcgcg tacggccagt tctcgagccg cgacgacacg gagcggggtg
    14881 gcgacctgga tcttccggat gcgtccgacc ggctgcaggc gggcttccgc ggagactggg
    14941 gagatgcctc cacggccatg acgctctcgg gcgacctcta tcgcgggaac atcacgcagc
    15001 cctcgcccgg tggcgatacg actcgggtcg tttccggcgg gaacgtcctc gggagctgga
    15061 cgcacaacta caagaccgga tcgcagcttc gcgtgcaggg ctatttcgat tacagcgagc
    15121 gggaacagcc ggacgcgatc catgaatcac tccagacctg ggacatccag gcccagcacg
    15181 gttttcggcc cggcgagaag cacgacgccg tctggggtgt cgagtaccgg taccaggccg
    15241 atcgcgtcgc caatctcacg cccctcatcg gattcctgcc gcccgaccgg gatctcagaa
    15301 cagcgtccct gttcggtcag gacgtgttca cggtgcacga gcgcgtggag ctgaccggcg
    15361 gcgttcgctt cgagcgaaac gagttcacgg actgggagtt cctcccgaac gcgcggctcg
    15421 cctggaagcc tcaggacgcg cgcctcctct gggccgccgc atcgcgcgcc gttcgcgcgc
    15481 cgtcccgcat cgatcgcgag atcttctcgc ccgtcacgac cccgcatctc ctcctcgcgg
    15541 gcggtccggg attcgagtcg gaagtcgcca gggtgttcga gctgggctac cgcgatcagc
    15601 cgacgcgcga cctttcgttc tcattgaccg gattccacca caactacgac gacctgcgct
    15661 cgctcaccac ctcccctgac ggccccatct tcgcgaacgg aatcgaggga acgaacacgg
    15721 gcgtcgagat ctgggggagc tatcgcgcat ccaccgcgtg gcgattcacg gccggatggg
    15781 ttcagcagtc gatcgatctc gatgtcgcgc ccggacactc ggcgctcgcc ggcacggcca
    15841 cgctcggcaa cgatcccaag tcgtggggcc agctccgctc ggcgctcgac ctgggcgatg
    15901 tggacgtgga cgccgcggtc cgctacgtgg gccgcttgcc cgatccggag gttccgtcct
    15961 acgcgacgct cgacgctcgc ctgggctggc acccgacgaa ggacttcgag ctgtcgcttt
    16021 ccggatggaa tttgacggga ggctcccatc cggaatgggg cgtcccaggc gcgcgacccg
    16081 acttcgagcg gagcgtgttc ttgaagatca agatgggctc ctaggcctct cgagggtgag
    16141 acgcttccat ccatgattcg ctggatcctc ctctcttccg cgctcctggc catcctctct
    16201 gggtccgcgc gcgcccagag tgacgattcc gacctggagc gccgggtcaa agccgcctac
    16261 ctctacaagt tcgctgggta cgtcgagtgg ccggactcct ccttccccga tccgacgacc
    16321 cccgtccgga tcgcggtgct cggcgacgac aagctccggg cggagctcgc caccatggtc
    16381 ctggggcaca cggtgaacgg gcgtcccgtg gtcgttctgg ggtacaccga ggaggactcc
    16441 ctggagggcg tccacgtgct ctacgtggga cggcaggaaa cctcgcgcct tcccaagctc
    16501 aaggacgagg cgcgcaagcg gtccgttctc ctcgtctcgg aaggagatga cgcgatcgat
    16561 cagggaagcg tcatcgcgtt cgtgctgagc cggcggcggg ttcggttcga catctcgatg
    16621 gcctgtgcgg ccaagtccgg gctcacgctg agctcgcgcc tcctgtcggt ggcgcgcacg
    16681 gtggtgacgg cgggatcatg atctttggcg ggattcgccg gtcgatccgg cgcaagttca
    16741 tgctcgtggt gatggcgacc acgctcgtgg cgctcctcct cgcctcgatc gcgctcgtca
    16801 cctacgacct cctcacctac aagcgcacgt gggtcgcgga cctctcgacg caggccgaca
    16861 tcctggggct cgcgagcgcc cccgcgctct ccttccagga tccaaagacg gcgcaggaga
    16921 acctcggcct tctctcggtc cgcccgaacg tgacggccgc agcgatctac acgtccgatg
    16981 ggcggctctt cgcttcgtat cgtcgggact cgacggacac cacgcctttg ccgccgcatc
    17041 ctggaccggc aggacacgag gccggtacga gggagatctc gctcttccgt ccgatcgtga
    17101 ctccggacga gcagctcggc tgggtctatc tacgcgcgcg ctacgacctg accgggcggc
    17161 tcctgagcta cctcgccatc gtggtcgggg tcatgctcgc gagcttgacg gtcgccgcgc
    17221 tcatgtcggt cgcgctccag gcgtccgtga tccgaccgat cctcgccatc gcggacgtcg
    17281 cgcacgaggt gaaggatcgc cgcaactacg ccgcccgcgt ccccaagacg acggaggacg
    17341 agaccggcta cctggtcgag accttcaacg acatgctcgg cgaggtgggc cagcggaccg
    17401 aggatctcga gcgcgcgaac cgccggctcg agcaggaagt ggccgtgcgg cagaacgcgg
    17461 agcacgcgct catcgaggcg gatcgtcgga aggacgagtt cctcgcgacc ctcgcgcacg
    17521 agtttcgcaa tccgctcgct cctctccgga acgcgctcca tatcctgaaa gtggcggggc
    17581 cgggctcgga ggacggaaag gtcgcgcgcg acatcatgga tcgccagctc cggcagctgg
    17641 tccggctcgt ggacgatctc ctcgacgtct cgcgcatcac gacgggcaaa gtcgtgctcc
    17701 agcgcgaatc cgttccactc gcgacgattg tcgagagcgc gctcgagacg gtgcgcccct
    17761 tcgtcgccga gcgcggcatc gcgctctccg tcacgatgcc ggacctgccg ctcatgcttc
    17821 gcgccgatcc gacgcggatg gcgcaggctc tcacgaactt gctccacaac gcggccaagt
    17881 tcaccgacgc tggggggcgc atcgatctgc gcgtggagcg cgagggcttg gaagggatca
    17941 cgatcaccgt ggcggacacg gggatcggga tcccggcgga catgctcccg ttcatcttcg
    18001 acatgttcac gcagctcgac cgctcgctcg agcgccccta ttccggcctc ggcgtgggat
    18061 tgtcgctcgc gaagcggctg atcgagctcc acggcggatc gctcgaggcg gcgagcgagg
    18121 ggcaaggcaa gggaacccgg ttcacggcgc gccttcccgg gatcgtcgtg ttcgagcccg
    18181 gccccatggc ggcctcgaac gatggcggcg ctcccgcggc gcccgcgcgc cgacgcgttc
    18241 tactcgcgga tgacaacatc gacttcacgt cgagcttcgc gctcatgctc cggagcatgg
    18301 gccacgacgt gatcgaggcg cacgatgggg ccgaggcgct tcaagccgcg cgcgccatcc
    18361 agcccgaggt cgcattcctg gacatcggtc tgcccaagat gaacggctac gatctggcga
    18421 ggcgcctgcg cgaggatccg gcgacgcgcc gcaccgtcct cgtcgccgtg accggttggg
    18481 gccaggatgg cgaccggagg ctcgccgcgg aggcgggctt cgaccttcac ctggtcaagc
    18541 cggtcgagtc cgcgcagatc gagacgatct tcgcggggct cgcttcgacg ccgcttcgcc
    18601 ccgcgtagcc cggaagcgcg ccgcctttgc tctagttgcg ccccttcgtc ccgtcccgca
    18661 gcacgatctg ccccttccgg ctgtagtcga tcgactcgcc caggatgcgc gcggcctcct
    18721 ggggcgcgtg gaagccgttc gagttctccg cctccacgaa gtcgagccgg aactgggcca
    18781 gacgctggta gcgccggccg gcttcgagcc ggacctcgtt ctgcccgacc gcctgcgcgt
    18841 gcttcaggtc ctcgatgaga tcgacgagcg cgtccattgc ctggttccgg agcttgaagg
    18901 tgcggtcctg gatgatttcg acccgctgtc gaagctcggc ctccgggaac ttgtggcagg
    18961 tctggcaggc gcggttgatg ttgaggagcg ggctctgcac gtgatgatcg ctgatcttga
    19021 gcgcgccttc ccgcatgtac ggcatgtggc agtcggcgca cgccacgccg gaacgcgcgt
    19081 ggattccctg gctccacatc tcgaactccg ggtgctgcgc cttcagaacg ggcgcgcccg
    19141 tgtcggcgtg aatccagtcc ttctgctgga tctcgtcata gtaggcctgg atctcctcga
    19201 tcttgatccc cttcgcccac ggatagacga gccgcttctc cttgcctttg aagtagtact
    19261 cgacgtggca ctggccgcag acgtaggagc gcatctcctg gcgtgtcgcg tccttgttca
    19321 cgtcgtagtt ctggattccc tgcgacgcct tgagtgcgcg aattccctcc atgaagccgg
    19381 ggcgcgtcac gcggagctcc atcgtctgcg gattgtggca gtcgatgcag gcgatcggat
    19441 gcgtgacgag cttccgcgcg tccgcatagg acatcgcgtt catgcgctcg aagccgcgcg
    19501 cgaggtcgcc gtctccgagc ttccggtacg ggaccacgat cgaggcgtgg cagtggaggc
    19561 aggtgcccgg ctgcgtggtg acgagctggc gctcggtgaa ggtctggtcg tccagcatgt
    19621 aggcatgccc gcgctcctca cggaagtcga ccgcgaaggc atagcccgcc cacatggtct
    19681 tgagcctcgg gtcctgctcc aggcgcgacc tggcgacgac ggagcgggga tccgccgccg
    19741 tgggcgtccg ctcctccgcc tcgcttccgc cgtagcgcgt ccgcaccatg tcgaccgtgc
    19801 gcttgtagcc gtcgtattcg gccgggaagt tcttccccca gacggccgga tcctccgtgg
    19861 tctcgttgat ctcgaccacg cgatagaacg gattccgccc ctcggcctgc ctctggaaaa
    19921 tgttgatgag aagggccgcg atgcctccgg ccgcgatcgc ggcgacgacc gcgacaatga
    19981 gtgccgtacg gaccggggat ccaccgcctc gacgcatagc gctctctcct tggctctagg
    20041 ggtgcccgac gctgccgtgg cagcggatac aagacggatc gctggacgga tgccccgccg
    20101 tcatcatagc gacgatggga ccgtgacagc tccggcagtt cttttcgacg atcgctcgat
    20161 tgtgaggctt gatctggatg ggctcgggga agtcgccgag cgtgaagtgg agcgagtgcc
    20221 agaagccgtt ccgcgccttc gtggtcacct tgccgatggg atcgtgcggc gtgtggcagt
    20281 cgttgcaggt cgcggtcgca tggtgcgtgg accgcaccca gccgtcgtat tgctcccgca
    20341 tgatgtggca gttggcgcag gcgcggggat cgttggtgag gtacgcgcct ccgtgcgcgt
    20401 acgtgaaggt gtagagcccc gttcccaccg ccacgccgac ggcgaggccg gcgagcacgg
    20461 ccggaacgtc acggatcgac cacgagcgcc caccgcggct ccggggcgtc agacgacctc
    20521 cggcgcgctg ggatgcggcg cgcaccacgg attcggatgt ggtccctttg gaattcgatc
    20581 gtgcacgcgc cccctcgtcc agccccgacc cgtgcgggag catagaggga ccgcgcgcga
    20641 ggggcaacgc cgttgggtgg cgcagcgcta cgtagtggcg gcgagctccc gaaggccctc
    20701 gagggccgtt cggggcgccg cgtcggttcc gccgctttcc ggcaaagcgg cggaactcga
    20761 ctcagctttg taagaccaac aagcccttcg ggccgaaaac gctatacggc tcgccgtccg
    20821 ctgatgacgc tcagcaggaa cacgacgatg gcggcgacca agaggaggtg gatgaacccg
    20881 ccgacggcca ccttggtgat cagccccaaa gcccaaagaa caacgagaag taccgcaatc
    20941 gtaacgagca tgtctgaatc ctcctggccc cccttggggg tccacgttgg acgccccgcg
    21001 cgcccatgat ggacggagga agcaaggagc ttgccggtgg gcgcgcgggg ccgattctcg
    21061 gcctcagagc ggcacttagc ggaagcgcat cttgatcttt ccccaggtcg tttgttccgt
    21121 cgcggtggga cagtcgttca cggcgccggg cgtggggttg caacccgttc ccgcgggcgt
    21181 cccggatccc gcgtagcggt tgagtccgtc gaagagaacc caggcgtcgc tggggccaag
    21241 gtgccccgtc gatcggtccg tcgcgccctc gatggagttg taggtcttgc tctccacctc
    21301 gaccgtatcg gacccctgga tctggaaaag gcggaccgta tcccccgagt tgttcaggct
    21361 gagcccgctc acggtccggc tctgcgcgcg ctgccaatcc tcggccatgc gtcccgtgac
    21421 gaggatcacc gcgttcggcg cgagggaacc cgagaacgcg tagcggatgg tggaatcggc
    21481 atccgagacg aggtagcccg cgagtgtctc gaccgaagcg ccgttgttcc ggatctcgag
    21541 ccactcgtcc tgccgcgcaa cgaacgcgcc gtccccatcc caatcgcgcg agggtcccgc
    21601 gaggatctcg ctcagctgga tggacggatt ggcggcggcc gtcagcgcct ccgcccgttt
    21661 caccaccggc tcgtgtcgca gcatccccgc gaccaccgcg atcgccacgg tcagcacgag
    21721 cacccacaac gcaatcgccc acgaacgcac catgatctcc ccctttcagg agtgtggttg
    21781 gcgtcggggc gaaacggatt tgtccttcgg ggcggcgtgg ggagtgtaaa gggtgcacgc
    21841 tgggagtgat gatcggggga gagctaacgg tgccggaatt cgaagcgtgc tgtcaagcgc
    21901 gcatcatgcg ttcgatctca tttacgacag gtgctacacc gactcccgat gcctcgtgcg
    21961 ccagatggcg cgcggcgcgc cctggtcggg ggtgagtctc ggcagattcc agaacgcggg
    22021 gaggacgagg aggagcgcca tcgctccgcc ccagagcggg ctcgacagcg cggcggccac
    22081 gcccggcgtg ttgagccaga cggcgagcac gattgcggcg acgagcgcgc cgcggagata
    22141 accgccgccg agaaacgcga cggtgatccc cgcgaacccc aagggcacgg gccagcgctc
    22201 gagcgcgacg gcggggtcgg tccagagctg gagcgcgccc acgagacccg cgcacgcgcc
    22261 gcctgcggcg atcgtgagcg tctgcatcgc atccacgtcg acccccgccg cgacggcggc
    22321 gagtggattg gagccgacgg cgcggagctc gaacccacgc acggacgcgc ggaggagatg
    22381 cgcggccagg agccccagcc cgataccaag ggccagaagc aagattcccc acgccatcac
    22441 gaacgacgga gccaggtgga gccctggcgc ccaggcgagc gtgcgcgcca gcgtgccgag
    22501 ctggatctcg agtgcgggcg cggcggcggg ctcgacgccg aacgcgcgcg ccacgggaat
    22561 gagcatggcc gcgaggagga gcccgctcag gatttcgtgg atcgcgagct tccgcttgag
    22621 ccacgccatc accaggccca cgaagagccc gccaccggcg cccgcgatca ggcccaagag
    22681 tccctgcgcc ccggggagga tgtcggcgct ccgcgcgatc agcgatcctg caatgaagcc
    22741 ggcgaggaac tgagcgtgaa tgccgaaatt caggagaccc gcgtggagcg ggaagaggaa
    22801 cgcgagcccg agaaagagga gccccgccgc tgcgagcacg atgtctccgc tccacggcgg
    22861 ccatccctgg ctcgaccccg cggcggcggc gatgagatag cttcccgtga agagcgcgac
    22921 caggagcgcc gcgttcccga gcagcaccca tggaccgccg cgcgagcgtc ctggaatcac
    22981 gccgccgctc ccgacatctc gtcgatgatc atctcgcgcg tgagcgtttc ggtgaccggg
    23041 atccagacga agcgccctgc cgcgaggacg gcgaggcggt ccgcgacgcg catcgcttcc
    23101 tcgggctcct cggtcagcca gaggagaccg ctcccgcgct cgcactggag cacgagcgcg
    23161 cggcgcacgt attcctgcgc ggccagcgag aggccctgcg ccggcgagcg cgcgaccatg
    23221 agcaccggat tgcgaagcac ctcgcgcgcc acgatcaccc gctggcgatc gccgagcttg
    23281 agctggcgga agcgatggtg ggcctcggcc tcgggaatct cgaagtcacg cagcgtgcgc
    23341 accgcgttgc cgcggatcga gtcgtggcgg aggaatccct tccgtgcgaa cgggggcccc
    23401 gtggtctggc cgaggatgag attttcctcg atcgtgaact cggggatcgc ttgatcgcgc
    23461 gcatgcggcg gattcacgaa gctgatcccg gactccacgc ggtgccgccg cgtggacagg
    23521 atcatctcct tgccgaggaa ctgcatgctc ccctgctccg gcgagcggct cccgaggagg
    23581 acgtcgtgga gaaggttgag gccgtccgcc ggcgcgccca cgatcgcgag cacctccccc
    23641 gccttcacct cgaactcgag accggcgagc gacgtttcgc ggccgcggcc gcggagcacg
    23701 agatcctcga tgcgaagaag aggctcgccc ggcgtgtgat gtccgctcgg agtcctccgg
    23761 atgcccagcc cgcccgtcca tcgctcgacc agggttccct cggagcactc gtgcgtcgtc
    23821 accgtgacgg gctcggcgcc gcgccacagg atcgtgaccg cttcggcctc gggataggcg
    23881 tcgcgcggga aggcgctcgt gatgacggcc gggatcttct cctcggatgc gacgcggcgg
    23941 atggtcgcga ggaagtgccg cgcttcgtgg ggaccgagct cggacgtcgg ctcgtcgagg
    24001 agaaggaggg ccggctgatc ggccagggac gcggcgagct cgagaaggcg ccgctcgagc
    24061 ggacggagcg tctcgaccgg gacgtcgagg agaccttcga gcgcgaatcg agccgcgagc
    24121 gccctcgcct cctcgcgcgc ctgcttccag tggatgaaca tccccatccg cttgggctgg
    24181 cgatggagga tcaggcggtc gaggacgcga atctgcgcgg agccggcggg ctgcgggacg
    24241 agggctgccg gtgcgggtgc gaggagcgtg ccgcgctcga gcttcctccg tccggcgagc
    24301 gcttcgagaa tcgcggtctt cccagtatcc cgctcgccga ggagcatgtg cacggagccg
    24361 ggaccgaccg aaaagctcag atcctggacg agccagcgcg gcccgtcggc cagcgccatt
    24421 cccttcacgt cgagccgcac gttcttgtcg accacgttcc cctcctcccg tcacggccgc
    24481 cacgcggcaa atcttaacgc tactctcggc gcctattgcc tctacctcgc gccgcggcta
    24541 ctcccgtcga actctggtcc cggtagtccc ctcgagcgct tccgtgagct tggcgggcga
    24601 tgtgatcacg acctcgcgtc cgccgccgcg cacgaactcg atcgcggcct gcaccttggg
    24661 ccccatgctc ccggccggga aatgcccggc ggcagcatac tccgaaagct cctccgccgt
    24721 cgcgcacggc aattcgcgct gctccggctt tccgtagtgg agggccaccc gatcgacgcc
    24781 cgtgatgatg cagagcatct cgcagccgag gagccgcgcg agcaggctcg acgcgagatc
    24841 cttgtcgatc acggcctcga cgccatggag cgtgccgtcc cattgccgcg ccacggggat
    24901 cccgcctcct ccggccgcga tcacgatcgc gccgtcctcg acgagcttcc ggatcgccgt
    24961 gatctccacg acctcgagcg gcttcggcga ggccacgacc cggcgccacc cgcgcccggc
    25021 gtcctcggtc acggtccacc ccttctcctt cgcgaggagc cgcgcgcgct cctgcgtgta
    25081 gaaggggccg atcggcttgg tcgggcggcg gaacgcgggg tcccgctcgt ccacgatgac
    25141 ctgggtcacg agcgtcgtga ccggccgctg gatgccgatg cggcggagct cgttgtcgag
    25201 ggcctgctgc atcatgtagc cgatcccgcc ctgggagtcg gcgccgcaca cgtcgagcgg
    25261 catcgggggg atctgatccc gcgcggcctc gttccggatc aggatgttcc ccacgatcgg
    25321 accgttcccg tggctcagga ccacccgcac accctgctgg atgagcgcgg cgatcggctg
    25381 catcgtgagc cgcgtgatcg cgcactgctc gtcgaacgtg ccggtcccgc gcgcgggcag
    25441 gatcgcgttc ccgccgaggg tgatcaggat gctatggggc agccggtcca atcagcgcgt
    25501 cacgttggcg tgcgcttgcg cgaaggcctc gaaccgcttc aatccttcgc ggatgttctc
    25561 gatggagttg gcgtacgaaa agcggacgta gccctcgccg ttcgatccga acgcggtgcc
    25621 ggagagcgcg gccacgccgg cctcctcgag gagcgcggtc tcgacctgcg agctcttcat
    25681 gccgagcccg gtgatgttcg ggaagacgta gaacgcgccg gacggcttcg cgcacttgat
    25741 ccccggaatg gcgttcagcc cctcgacgat ggcttcgcgc cgccgccgga actcctccac
    25801 catcgcgcgc acctcgtcct gcggcccgag gagcgcaccg ggagcggccc gctgcacgaa
    25861 ggacgtcgcg cacgagtaga cgttggtcgc gaggcgcgcg acgtgcttgg cgagcgtcgg
    25921 attcatgact ccgtaaccca gccgccagcc ggtcatcgcg taggtcttgg agaatccgtc
    25981 caggatgatc gtccgctccg ccatgcccgg gcgcgtcgcg atcgtctcgt gcgtcccttc
    26041 gtagaggatc ttggaataga tctcatcgga gaggacgaag acgtcgcggt cccgaaggag
    26101 cgacgcgatc tcgtcgagat ccttcggcgt gagcacggag ccggtcgggt tgtggggcga
    26161 gttgagcacg agcatccggg tgcgcggcgt gaggagcttc tccacgtcgg ccgcgccgaa
    26221 gcggaacgag cggtcctcgc ggagcgcgag cgagacgggc gtgcctccgg cgaaccgcac
    26281 cgcggactcg tagatcggga agccgggatt cgggacgatc acctcgtccc ccgggttcac
    26341 gcacgcgagc acggcgaacg tgagcacggg ctttccaccc ggcgtcacca cgacctgctc
    26401 cggtgcgacc gggagtccgc gatccttcgt gatgtactgc gcgatcgcct cgcgaacctc
    26461 gggaaggccc gccgaggggc cgtagtgcgt gaagccttcc tcgatcgcgc ggatcgccgc
    26521 ctgacggatc gaggccggcg tatcgaagtc gggctcgccg atctcgaggt gcacgatggt
    26581 ccggcccttc gcctcgagcg cgcgcgcctt ggcgagcact tcgaaggcgg tctcggtgcc
    26641 gagctgactc attcgatccg cgtagcgcat ggacttctcc tcgtcagtcc gcccgaatcg
    26701 gggcgttcgc attcaccatc attcccggcc ggattcgcga agctcccggc cgacccagat
    26761 cgaggccgcg gtcgcgcgct cgccggagac atcgtctcgc tgctcgacga gtccgcccag
    26821 cgtccagcgt cccagcttcc aggcgcctct cgcggtcgcc gcgacgcccg gccgcgcggt
    26881 cgtcaagagc gccgtcgcgc cgctcgcata gagcccgctc gaccaggccg cgccgtccct
    26941 gacggcgcga atcccttcga gctggatctc gagcgtggcg cgctcgcgcc atgcgagcga
    27001 gagccggccc ccgagggacg accctcgcct cacgcccgcg accgtctcct tgtcgcgccg
    27061 cgacgcgaga agcgtgagcg tccggccgtc cgcgcgggcc acctgcatct cggcgaccgc
    27121 gtaccgctcg atcgacgcgt gaaagcccgc cgcgttggag aagctctcgg tccgcgcgct
    27181 cccgatccga aacgagagcg ggccgtaccc cgccggtgcg atccggccct cca
//
LOCUS       DASTKC010000042        16702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_976353, whole genome shotgun sequence.
ACCESSION   DASTKC010000042 DASTKC010000000
VERSION     DASTKC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16702
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976353"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFR25_01740"
     CDS             <1..893
                     /locus_tag="VFR25_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HET9939802.1"
                     /translation="GDAEFQRIIDERLSPLYVSVHATDPEVRVRLMKNPKSGRIMEHI
                     DKLTAAGIEMHTQLVVCPGINDGAILERSIRELGERHPAVKTIAVVPVGLTKHRERLP
                     QLRTFTADDARLALEETARQATAFKKRFGTRIVFAADEMYVLAGEAVPSARAYEGFPQ
                     LENGIGMLRSTMDRWAKGEDDIRPRNGARERVVLVTGTSAGPTIERLLDERPPAGVDV
                     SVCTVVNDYFGDTVTVSGLLVGADIERALLEHGPADRVLLPPNCLKEGEVFLDDRTRT
                     DLESRLGVPVQIGFDAKIAA"
     gene            890..2212
                     /gene="der"
                     /locus_tag="VFR25_01745"
     CDS             890..2212
                     /gene="der"
                     /locus_tag="VFR25_01745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9939803.1"
                     /translation="MSEALPIVAIVGRPNVGKSTLFNRLLGQRRAIVDELSGLTRDRH
                     YAETEWSGRKFLLVDTGGIDPGSAHPIQRQIIHQTTQALEEADVSLLVVDAAQGITAL
                     DREVADRVRKGGRPMLLIVNKADSAAREDEAAEFYSLGVGTPIPVSALHGRNAGELLE
                     EIVHRLPTVEAEPVDETAIRIAVVGRPNVGKSSIVNRLLGSERMVVDSVAGTTRDAVD
                     TAFTKDGKRYVIIDTAGLRRERKVTDPVEFYSVTRALRALSRADIMLLIIDVSREPAK
                     QDAKLAALAEEQRKGLIAVFNKWDLVEDPVEARALIEEEFLKQYPFLDFVPRLYVSAQ
                     KGTGLKKILPAAATVHEQYARSITTSQLNDAIQGILGRVQPPATKGGKHLKFYYAAQT
                     GTRPPTFSLFVNNPRYRQQNYVSYLERGLRERFGFQGTPIVLEWKASH"
     gene            2247..2885
                     /gene="plsY"
                     /locus_tag="VFR25_01750"
     CDS             2247..2885
                     /gene="plsY"
                     /locus_tag="VFR25_01750"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET9939804.1"
                     /translation="MPLLVPSILIGFLLGSIPTGLLIGRARGVDLRTQGSKNIGATNA
                     FRVLGPKWGGLVFALDVVKGLTAVLLVRHFGPSWGASHDAVLTAMLAAGVAAILGHVF
                     SPWLRFKGGRGVATSLGVFLGIVPVPTLLAFALWVILLLVSKRVSVGSIGAALAYPFL
                     VYALAKDVPRGVVTAVTAAVALLVILRHTANIRRLLAGTEPPIIGARREGRS"
     gene            2882..3907
                     /locus_tag="VFR25_01755"
     CDS             2882..3907
                     /locus_tag="VFR25_01755"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET9939805.1"
                     /translation="MKIGVIGGGGWGTALSIVLESRGHQVTLWVYEADLAEHMRRSRV
                     NERFLPEVSIPEAVTITSSLGEAVAGATSLLFVPPSHALRSTAARLVEEAPDLTRVEW
                     VTVATKGIETRTLKRPSEVLAEILPAPLRERIVVLAGPSHAEEVARRIPTLIVAASRD
                     AALAQRTQEAFHTDWLRIYTNDDVVGVEIGVALKNVIAIAAGIADGLGFGDSTKAALL
                     TRGLAEMGRLAAKLGARPETLSGLAGMGDLIATAGSRHSRNRRFGEAVGRGATVKEAL
                     AASPMVVEGVATAEAAVALGQREGVELPIIEQVHAILHEGKSARTALRELLTRDLKSE
                     APTMSRR"
     gene            3912..4313
                     /locus_tag="VFR25_01760"
     CDS             3912..4313
                     /locus_tag="VFR25_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9939806.1"
                     /translation="MKSLPAGRLYYSISEVSDLIGVKPHVLRYWETQFKMLRPKKGRG
                     GARMYRRRDVETLYDIKQLLYDQRFTIAGARRRILDGKQEDGKEQIELPFSKLDREEM
                     MRSLKSDMEHLLTMLRDEAVGAKVAKGRSKR"
     gene            4404..4480
                     /locus_tag="VFR25_01765"
     tRNA            4404..4480
                     /locus_tag="VFR25_01765"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4438..4440,aa:Pro,seq:ggg)
     gene            4552..5385
                     /locus_tag="VFR25_01770"
     CDS             4552..5385
                     /locus_tag="VFR25_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939807.1"
                     /translation="MTDFGYLLASTTACTLPRPWSEALAAAFADLYTWIHPARARAID
                     GRLRRIWSETGRPGSPPSPRETYRAFARAVLDFLAARPGKRPRVRLDDEARAILDQVR
                     ASGRPTVIVSGHFGPWEVALQWVAREVGSVHAIARAHRSRAVERFFAARRAAFGVRTL
                     SGTRPASAALEKLRAGGWIAALADRGSRPRRATAPAAASGGLVPLDPAPLLLARRANA
                     QILAGVAWRAPDGAVEVRFHPPFSLDIGMHEAEATLQRFFDAHVRAHPTQWFHWAGNA
                     G"
     gene            5382..6047
                     /locus_tag="VFR25_01775"
     CDS             5382..6047
                     /locus_tag="VFR25_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9939808.1"
                     /translation="MKLVSVIPARDAAGTIGDVVRSLRAKFPESEVVVVDDGSTDETG
                     ARARDAGAVVVKHEVKLGKGAALQSGFDEALRRGADAVLALDADGQHDVNAAPGLIAA
                     LTDADLVIGSRERDRTGMPWLRRKTNDVTTWWTSYLAGQRVPDSQSGYRAIKAPVIRA
                     VRPVSRRFEYESEFLVRAARQGFRIGQAPIPTLYNAPGSHIHPVRDTMRFIRLVLRLL
                     ASR"
     gene            6047..6808
                     /gene="surE"
                     /locus_tag="VFR25_01780"
     CDS             6047..6808
                     /gene="surE"
                     /locus_tag="VFR25_01780"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET9939809.1"
                     /translation="MEILVTNDDGIGAEGIRALAKAMGTLGNVTIVAPDREQSATSHA
                     LTLHRPLRMRKISERELGVDGTPTDAVLLGVHGFLKERPALVVSGINHGPNMGNDVLY
                     SGTVAAASEGTFLGIPSVAISLATWDAADFGPSARVAQALVAQLLRRGLPAKTCLNVN
                     IPPIPFEEIKGVRVTRLGKRVYRDVIVEKTDPRGKLYYWIGGEEPTWENDDSSDFTAV
                     ERGYVSVTPLTFELTDYKELVDLEAKGLSLDESAS"
     gene            6792..7460
                     /locus_tag="VFR25_01785"
     CDS             6792..7460
                     /locus_tag="VFR25_01785"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET9939810.1"
                     /translation="MNPHHSDLSVARRRMVEEQLRTRGIHDLHVLRIMQAMPRHLFVD
                     DTLSASAYSDHALPIGDDQTISQPYMVALMTQALDLTGNEKVLEIGTGSGYQTAILAE
                     LAERVFTIERIPAIAQAARERLTALGYSNIVFRTADGTLGWRDMAPFDRILITAGAPH
                     LPTFLEEQLHLGGIAVVPVGQSENQSLIKATLTPEGLTRRVLCGCTFVPLIGREGWAA
                     GEVG"
     gene            7534..7610
                     /locus_tag="VFR25_01790"
     tRNA            7534..7610
                     /locus_tag="VFR25_01790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7568..7570,aa:Pro,seq:cgg)
     gene            7643..7951
                     /locus_tag="VFR25_01795"
     CDS             7643..7951
                     /locus_tag="VFR25_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET9939811.1"
                     /translation="MRFRVPVVTETQKARLEARVTGRVQGVGYRWFVQAEADRLSLGG
                     SVRTLPDGAVAVAAEGPRPKLLVLLKRLHEGPPAARVERVDAEWLTPHGQTEFRILSG
                     "
     gene            7960..9015
                     /locus_tag="VFR25_01800"
     CDS             7960..9015
                     /locus_tag="VFR25_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9939812.1"
                     /translation="MPENPRRFVSELSEAMRAGLERGALTDDDLDPLLHSSDFDAGAF
                     DLFLAEARRQGIRLPDEAKPDNLNPPDSDHTIGSLERRYLKEIQRYPILARDEERRLW
                     EAMRVGNQDARKALILAYLRLVVSIARAYRNRGVEFLDLVEEGNLGLITAVDRFDVDR
                     GIHFSTYGTWWIRQALARGVANQSRTVRIPIHVLQMMRRFVATQRRLESELRRKPEAS
                     EVAQEMGLPEARVYRLESLVQSIHTLDVDMSNEAFHGLIESESIEQPPSLDEIVEMQI
                     RDQQLNEMLKRLSGREEAILRLRYGFYDDRPRTLAETGEHFGLSRERIRQLEERALLK
                     LRHLLEAETSEARASVH"
     gene            complement(9012..9644)
                     /locus_tag="VFR25_01805"
     CDS             complement(9012..9644)
                     /locus_tag="VFR25_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939813.1"
                     /translation="MRAIIIALAIAAALPGCAQNPKTAWEIVPGERLGVLKLSDSERE
                     LGAGYGAQNVRHVTVSLGEGEALSGTLLYPEDPEKRVEIVWMNVTARRYPMHAILRGT
                     RSRWTLPRGITLGTTLAELERLNGHPFTMAGFGWDYSGAVTDWEGGALSSVLKGRAWL
                     YLEPAPGKDQDPSFAQVQGDRDISSTSPAMRALEPRITRIRVSLAEPRRN"
     gene            complement(9641..10498)
                     /locus_tag="VFR25_01810"
     CDS             complement(9641..10498)
                     /locus_tag="VFR25_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET9939814.1"
                     /translation="MTVRAIAFAFICGTSSLPVHSLAEQTETGGSTDSSESRFTREMS
                     TDRPDLTESPYTVEKGHTQVEIEAVSHGSETSDGVEVTSTGLAGFLIKRGIHRNADIQ
                     FGTSQLFRVTDFEFDRTLHPGEIDVSDNTGPGDLQIRLKWNFWGNDGGKTAFALMPFL
                     DIPTSHDSPGSALEGGLIAPLAIEGPAGFGLGVMGEVDWFDVQGERWTEWLTTATASH
                     DLVGGLGGFVEFAATFRVPDEGEWLGLFNTGATLAITPNTQLDGGVRLGITDVAPDFE
                     AFLGFSFRQ"
     gene            10594..10899
                     /locus_tag="VFR25_01815"
     CDS             10594..10899
                     /locus_tag="VFR25_01815"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HET9939815.1"
                     /translation="MKRYGFVVWIVCATALGCAGTAGHLAKPGKEIKAHGLLAAGAEC
                     PMLETSDGHRYALAGSLESFKTGDRVCVRGKVAGASICMAGEATLTIESIEAESACR"
     gene            10952..11266
                     /locus_tag="VFR25_01820"
     CDS             10952..11266
                     /locus_tag="VFR25_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939816.1"
                     /translation="MLLVREIMYCKPGKVKAFVEKTLAMQKLMKKLPNQPSMRVMTDF
                     CGEQFWMCVMQMEVPDMATFEKLMSGEGESQESMKEFENIMKGYHDLVDHGRREIYKI
                     EG"
     gene            complement(11330..11479)
                     /locus_tag="VFR25_01825"
     CDS             complement(11330..11479)
                     /locus_tag="VFR25_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939817.1"
                     /translation="MIRKLKSGQYRLYSIKKNPKTGRRRNLGTFSSRQAAEKHERAVQ
                     YFKRH"
     gene            11611..12063
                     /locus_tag="VFR25_01830"
     CDS             11611..12063
                     /locus_tag="VFR25_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9939818.1"
                     /translation="MKKSESASTLIDGKIKELGDWRGKTLAKVRKLIHQADPEIVEEW
                     KWMGTPVFSHDGIVCTGETYKSVVKLTFAKGASLDDPAGLFNSSLDGNVRRAIDIREG
                     EAIDEAALKNLIRAAVTLNLEGKSKPKSRPKTATAKATRKKAKRSSTA"
     gene            complement(12075..13520)
                     /locus_tag="VFR25_01835"
     CDS             complement(12075..13520)
                     /locus_tag="VFR25_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET9939819.1"
                     /translation="MRRTLVEPGAPIRPGVLLGLLAFVVGMVVSARAEAQRAAPQFPD
                     SSAAKQIERPDEGYVAVKRPWLAASEVVGVNALVWSYDRYIRENGTNPGFRIGFNSFQ
                     ENIQNGFEFDDNNFSTNMYAHPYHGDLYFNAARSNGMSYWGSVPYTWGGSFMWEYFGE
                     VHHPAINDWILTSMGGMALGEVFFRLSQMVTDNTATGSRRSWGEVGGLIISPVRGFTR
                     LVTGEFNRVGPNPPDRFPRSSRVSYRVGLRTSGQDQLWDADTTRPFIEVAAYVGDPFL
                     GDNKKPYDSFDFNFQININDAQTIGRVQTIGLLASSPVMGTDHSKHLIGFTQHFDYFN
                     NSDIELGGQSLAASLYSQFRAGEHFAVRTQLHMNALIMGATKDNYQTFTGRSYDFGPG
                     LAFKFGSTFYYHGHPYLMLTHSRFWIHSINGSVSDHLISTSRVRFDVPLTRGFSIGAD
                     YWLYTADRLYRDLPDVYERVPVLRTGISFNL"
     gene            complement(13520..14671)
                     /locus_tag="VFR25_01840"
     CDS             complement(13520..14671)
                     /locus_tag="VFR25_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939820.1"
                     /translation="MAPRLLMAHIVLAALFLSASALAAEGRSPIVLAFLYPLATNRSP
                     EVATSVHLSLLYGRVGSVDALGLHGVVTMTGGNARGVQLTGIYAQTYGKASGVHLTGV
                     VSYAMQDVSGIHLSGLANVAQGRLSGFEAAALANVAGEGMSGVQLSPVFNLADGGARG
                     FQVAGIANVVGRAFDGWQVATGLNYVTDQMVGLQFGGANAALDMRGTQIGVVNFATRT
                     RGFQMGALNFAEEQHGVPFGMVNIAGNGDVDWITYGSNLSAVNTGIGTSVRRFYSMLT
                     AGLPDLQGDVSSTLILTWNYGYAIPTGRGTSIGIDMGYAHYIPEKVDDPNENDRLHFS
                     LQARAMVERTFSRKAKGFAGGGVARIWDEYRLDAPAETEPLFFAGMALF"
     gene            complement(14671..15270)
                     /locus_tag="VFR25_01845"
     CDS             complement(14671..15270)
                     /locus_tag="VFR25_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939821.1"
                     /translation="MLILFLPTFTAQPAFALYEHVRTGWVAGIGYGWSKAHITTGDSL
                     HLESPWQDGSAPRLRLGHMLGRHAMLGYEQVQWLQVVGNADAFVRVSLQTFGAAITVF
                     PGDPKHVTGGIYLRAGAGLVNARVAFGKVPTGADSTHHEETVDESGAGFMFGGGYELR
                     ISKPAALALDVTANYHTVGKDFFDKAWFVPMTLGLNWYF"
     gene            complement(15327..16280)
                     /locus_tag="VFR25_01850"
     CDS             complement(15327..16280)
                     /locus_tag="VFR25_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939822.1"
                     /translation="MMPRARVRFGPWALAIAGTLALAAPAAAQQGPRPALGGHTFVST
                     DLVPDAFVRTYVRNSLGYTMTPEFNYPPIVVGGDTLLTLNGTLAYALLGMEYQHAIRD
                     WIAVRAALGMRTRIGTQVSSLVNEGVNVTSGIELGWLVRIRETPRTSLVGSLDVTRQT
                     LTLIDIRQFTEDVIDGIPDPSLIDNIPTVRSSAGLRFAWAMSRTFGLTALAEGSYGES
                     PRRDQVDSWENGFGASVDLDGGAAWGVPIGLVLAYRQTSQPALTTTDNGSVRQTLFRI
                     AYTGKPDFLIALDITGVLDRENREADSVWAGGAAFSLRYYF"
     gene            complement(16277..>16702)
                     /locus_tag="VFR25_01855"
     CDS             complement(16277..>16702)
                     /locus_tag="VFR25_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9939823.1"
                     /translation="TFVQGNTKLRSADADPGDERSLDLDLDYLSVPVVVKFGAAHGRT
                     YFAGGMDVGFLNSARVSGEGIDEDIEPLLHSVDVGALFGFGVVFPAGRSRLTTELRYV
                     QGLVNLRDAEQGLVQELPDRFHSSGLQLMVGILLPLGAR"
BASE COUNT         2904 a         5536 c         5565 g         2697 t
ORIGIN      
        1 tcggcgacgc ggagttccag cgcatcatcg acgagcgcct ctcgccgctc tacgtctcgg
       61 tccacgccac cgatcccgag gtgcgcgtcc ggctcatgaa gaatccgaag tcggggcgga
      121 tcatggagca tatcgacaag ctcacggcgg cggggatcga gatgcacacg cagctcgtgg
      181 tctgcccggg catcaacgac ggtgccattc tcgagcgctc catccgtgag ctcggcgagc
      241 ggcatcccgc cgtgaagacg atcgccgtgg tgccggtcgg actcaccaag caccgcgagc
      301 ggctgccgca gctccgcacc ttcaccgccg acgacgcgcg gctcgcgctc gaggagacgg
      361 cgcggcaggc gaccgcgttc aagaagcgct tcgggacgcg catcgtgttc gcggcggacg
      421 agatgtacgt gctcgccggc gaggcggtcc cgtccgcccg agcctacgag ggcttcccgc
      481 agctggagaa cggcatcggg atgctccgct cgacaatgga ccgctgggcg aagggggaag
      541 acgatatccg cccgaggaac ggcgcgcgcg agcgcgtggt cctcgtgacc gggacgagcg
      601 caggccccac gatcgagcgg ctcctcgacg agcgcccacc cgcgggcgtg gatgtctcgg
      661 tctgcaccgt ggtgaacgac tacttcggcg acacggtgac cgtgagcggg ctcctggtcg
      721 gcgccgacat cgagcgcgcg ctcctcgagc acggcccggc cgatcgcgtc ctgcttccgc
      781 cgaactgcct caaggaaggt gaagtgttcc tggacgatcg cacgcggacc gatctcgaat
      841 cgcgcctcgg cgtccccgtc cagatcgggt tcgacgcgaa gatcgccgcg tgagcgaagc
      901 gctccccatc gtcgcgatcg tgggccgccc caacgtgggg aagtccacgc tcttcaaccg
      961 cctgctcggc cagcggcgcg ccatcgtcga tgagctctca gggctcacgc gcgaccggca
     1021 ctacgccgag acggagtgga gcggacggaa gttcctcctc gtcgatacgg ggggcatcga
     1081 tcccggaagc gcccatccca tccagcgcca gatcatccac cagaccacgc aggcgctcga
     1141 ggaggcggat gtctcgctcc tcgtcgtcga cgccgcgcaa ggcatcaccg cgctcgaccg
     1201 tgaagtcgcg gaccgggtgc gcaagggcgg gcgcccgatg ctcctcatcg tgaacaaggc
     1261 ggactccgcc gcccgcgagg acgaggcagc cgagttctac tcgctcggcg tgggcacgcc
     1321 catccccgtc tccgcgctcc atgggcgaaa cgccggcgag ctgctcgagg agatcgtcca
     1381 ccggctgccc accgtggaag cggagcccgt ggacgagacc gccatccgga tcgctgtggt
     1441 ggggcgcccg aacgtgggga agtcctccat cgtgaaccgg ctcctcggct cggagcggat
     1501 ggtcgtggac tcggtggcag ggaccacgcg ggatgcggtc gacaccgcct tcacgaagga
     1561 cgggaagcgc tacgtcatca tcgatacggc aggactgagg cgggagcgga aggtcacgga
     1621 tccggtcgag ttctacagcg taacccgtgc tctgcgtgcc ctttcccgcg cggacatcat
     1681 gcttctcatc atcgacgtgt cgcgcgagcc ggccaagcag gacgccaagc tcgcggcgct
     1741 cgccgaggag cagcggaagg ggctcatcgc ggtcttcaac aagtgggatc tcgtcgagga
     1801 tccggtggaa gcccgggcct tgatcgagga ggaattcctc aagcagtacc cgttcctcga
     1861 tttcgtgccc cggctgtacg tctccgctca gaagggaacc ggattgaaga agatccttcc
     1921 cgccgcggcc accgtgcacg agcagtacgc gcgctccatc accacctcgc agctgaacga
     1981 tgcgatccag ggcatcctgg ggcgggtcca gcccccggcc acgaaggggg gcaagcacct
     2041 caagttctac tacgcggccc agaccgggac ccgtccgccg acattctccc ttttcgtgaa
     2101 caatcctcga tatcgtcagc aaaattatgt aagttacctc gaacgaggcc tacgcgaacg
     2161 gttcggtttc cagggcaccc cgatcgtgct cgaatggaag gcgagccact agcggaggtt
     2221 cggcgcccga ccctcggagg ctcgccatcc cgctcctcgt tccgtccatc ctgatcggct
     2281 tcctgctcgg aagcatcccc acggggctcc tgatcggccg cgcccgcggt gtcgatctac
     2341 gcacccaggg cagcaagaac atcggcgcga cgaacgcgtt ccgcgtgctc ggtccgaagt
     2401 ggggcggcct cgtcttcgcg ctcgacgtgg tgaaggggct cacggcggtc ctcctggtgc
     2461 ggcacttcgg accgtcctgg ggcgcgtcgc acgacgccgt gctcacggcc atgctcgccg
     2521 caggcgtcgc ggccatcctc ggccacgtct tctcgccatg gcttcgcttc aagggcggac
     2581 gcggcgtcgc cacgagcctc ggcgtgttcc tcggcatcgt cccggtgccg acgctcctcg
     2641 cgttcgcgct ctgggtgatc ctcctcctcg tgtccaagcg cgtctccgtg ggctcgatcg
     2701 gcgccgcgct cgcctatcca ttcctcgtct atgcgctcgc caaggacgtg ccgcggggtg
     2761 tggtgaccgc cgtgacggcg gccgtcgcgc tcctcgtgat ccttcgccac acggcgaaca
     2821 tccgccggct cctcgccggc accgagcctc cgatcatcgg ggcgcgccgg gaggggcgct
     2881 cgtgaagatc ggcgtcatcg gcggaggcgg atggggcacc gcgctctcga tcgtgctcga
     2941 gagccgcggc caccaggtga cgctgtgggt ctacgaggcc gacctcgcgg agcacatgcg
     3001 gcgctcgcgc gtgaacgagc ggttcctgcc cgaggtgtcg atcccggagg cggtgacgat
     3061 cacgtcgtcg ctcggcgagg cggtggccgg agcgacgagt cttctcttcg ttccaccctc
     3121 ccacgcgctc cgctcgacgg ccgctcgtct cgtcgaggag gctcccgacc tgacccgcgt
     3181 ggagtgggtc acggtggcga ccaagggaat cgagacgcgc acgctgaagc gcccgagcga
     3241 ggtgctcgcg gagatccttc ccgcgcccct tcgcgagcgc atcgtcgtgc tcgcggggcc
     3301 gagccacgcg gaggaggtgg cgcggcgtat cccgaccctc atcgtcgccg cgtcgcgcga
     3361 cgcggcgctg gcgcagcgca cccaggaggc attccacacc gactggctcc ggatctacac
     3421 gaacgacgac gtcgtcggcg tggagatcgg cgtggcgctc aagaacgtga tcgcgatcgc
     3481 cgccggcatc gcggacggcc tcgggttcgg ggattcgacg aaggccgccc tcctcacgcg
     3541 cggactcgcg gagatggggc gtctcgcggc caaactcggc gcacgccccg agacgctgtc
     3601 cggcctcgcc ggcatgggcg atctcatcgc gaccgcgggg agccggcaca gccgcaatcg
     3661 ccgcttcggt gaggccgtcg gacgaggagc cacggtgaaa gaggcgctcg ccgcttcacc
     3721 gatggtcgtt gaaggcgtcg cgaccgccga ggccgcggtc gcgctgggac aaagggaagg
     3781 cgtcgagctg cccatcatcg agcaggtcca cgccatcctt catgaaggga agagcgcacg
     3841 cacggctctt cgcgagcttt tgacccggga cttgaaatcg gaagctccca ccatgtccag
     3901 gaggtgatcc cgtgaagtcg cttccggccg gcaggctcta ctactccatc agcgaagtca
     3961 gcgacctgat cggcgtcaaa cctcacgtgc tccgttactg ggagacgcag ttcaagatgc
     4021 tccgtcccaa gaaggggcgc ggcggcgcca ggatgtaccg gcgccgcgac gtcgagaccc
     4081 tctacgacat caagcagctc ctctacgacc agcgcttcac catcgccggc gcccggcgcc
     4141 gaatcctcga cgggaagcag gaggacggca aggagcagat cgagctcccg ttctcgaagc
     4201 tcgaccgcga ggagatgatg cgctcgctca agtcggacat ggagcacctc ctcacgatgc
     4261 tccgggacga ggcggtgggg gcgaaggtcg cgaaggggcg ctcgaagcgc tgaggcgccg
     4321 ctatcggctt gagcggccgc cccttgccgt caaacacgcc ctcgtgtagc atcgccgaaa
     4381 cgcccggctt ttgaagcttc ggtcggggcg tagcgcagcc cggtagcgca cctccttggg
     4441 gtggaggtgg tcgcaggttc aaatcctgtc gccccgacca tttcgctgtc cgcagtcctg
     4501 ccgcctcggg gcacgccccg gggcgtcgtt gttctcgcgc gtgggggaca cgtgaccgac
     4561 ttcgggtatc tgctcgcatc gacgaccgcg tgcacgctcc cgcgtccgtg gagcgaggcc
     4621 ctcgcggccg ccttcgcgga cctctacacg tggatccatc cggcgcgcgc cagggccatc
     4681 gacggccgcc tgcgtcgcat ctggtccgaa accggccgcc ccgggtctcc acccagcccg
     4741 cgcgagacct atcgcgcgtt cgcgcgcgcg gtgctggact tcctcgcggc acgtcccggc
     4801 aagcgcccgc gggtgcggct cgacgacgag gcacgcgcga tactcgatca ggtgcgtgct
     4861 tccggccgcc ccacggtgat cgtgagcggc cacttcggtc cgtgggaagt ggcgctccag
     4921 tgggttgccc gtgaggtcgg ttccgtgcac gcgatcgcgc gcgcgcaccg ttcgcgcgcg
     4981 gtggagcgat tcttcgcggc caggcgggcg gccttcggag tgcgaacgct gagcggaacc
     5041 aggcccgcgt cggccgcact cgagaaactg cgtgccggcg ggtggatcgc ggcgctcgca
     5101 gaccgcgggt cgcgtccgcg acgggcaacc gcaccggcag cagcatcggg cggactcgtt
     5161 ccgctcgatc ccgccccgct cctcctcgcg cggcgcgcca acgcgcagat cctcgcgggc
     5221 gtcgcgtggc gggcgcccga cggcgccgtg gaggtccgct tccatccgcc attctcgctc
     5281 gacatcggca tgcacgaagc cgaagccacg ctccagcgct tcttcgacgc gcacgtccgc
     5341 gcgcatccca ctcagtggtt ccactgggcc gggaacgcgg gttgaagctc gtctccgtga
     5401 ttcccgctcg agacgcggcc ggaaccattg gagacgtggt gcgctcgctg cgcgccaagt
     5461 ttcccgagtc cgaggtggtt gtggtcgacg acggctccac ggatgagacc ggagcacgcg
     5521 ctcgcgatgc gggggcggtc gtggtcaagc acgaggtcaa gctggggaag ggagccgcgc
     5581 tccagtcggg cttcgacgaa gcgctccggc ggggcgcgga cgcggtcctg gccctcgacg
     5641 ccgacggcca gcacgacgtc aacgccgcgc ccggtctgat cgcggcgctg acggacgccg
     5701 acctcgtcat cggctcgcgc gagcgcgacc ggaccggaat gccgtggctt cgccgcaaga
     5761 cgaacgacgt gaccacgtgg tggacgtcct acctcgcggg gcagcgcgtc cccgattccc
     5821 agtccggcta ccgcgccatc aaagcgcccg tgatccgcgc cgtgcggccc gtgagccgcc
     5881 ggtttgaata cgaatcggag ttcctggtgc gcgcggcgcg gcaaggcttc cgcatcggcc
     5941 aggcgccgat cccgaccttg tacaacgcgc ccggatccca catccatccg gtccgggaca
     6001 cgatgcggtt catccggctc gtgctccggc tcctggcgtc tcgatgatcg agatcctggt
     6061 cacgaacgac gacgggatcg gtgcggaagg gatccgcgcg ctcgcgaagg cgatggggac
     6121 gctcgggaac gtcacgatcg tggcccccga ccgggaacag agcgcgacca gccacgccct
     6181 cacgctccac cgaccgcttc gcatgcgcaa gatctccgag cgcgagctgg gcgtggacgg
     6241 cacgccgacg gacgcggtgc tcctcggcgt gcatggcttc ctcaaggagc ggccggcgct
     6301 cgtggtctcc ggcatcaacc acggccccaa catggggaac gacgtgctct actccgggac
     6361 cgtcgccgcg gcgagcgaag gaacgttcct cggcattcca tccgtcgcca tctcgctcgc
     6421 cacctgggac gcggcggact tcggcccctc ggcccgcgtc gcccaagcgc tcgtcgccca
     6481 gctcctccgt cggggccttc cggccaagac ctgcctcaac gtcaacatcc cgccgatccc
     6541 gttcgaggag atcaagggcg tccgggtcac acggctgggg aagcgggtct atcgcgatgt
     6601 gatcgtggaa aagaccgatc cgaggggtaa gctctactac tggatcggcg gcgaggagcc
     6661 cacctgggag aacgacgact cgagcgactt caccgccgtc gagcgcggtt acgtgtcggt
     6721 cacgccgctc acgttcgagc tgaccgacta caaggagctg gtcgacctcg aggcgaaggg
     6781 actttccctc gatgaatccg catcatagtg atttgagcgt cgcccgccgg cgcatggtgg
     6841 aggagcagct ccgaacgcgc ggcatccacg atctgcacgt actccggatc atgcaggcga
     6901 tgccgcggca tctcttcgtg gacgacacgc tctccgcgag cgcctattcg gaccacgccc
     6961 tccccatcgg cgacgatcaa acgatctcgc aaccctacat ggtcgcgctc atgacccagg
     7021 cgctcgacct gacagggaac gagaaggtgc tcgagatcgg caccgggtcg ggctaccaga
     7081 ccgcgatcct cgccgagctg gcggagcgcg tgttcacgat cgaacggatc cccgccatcg
     7141 cccaggccgc gcgcgagcgg ctgaccgccc tcggctactc gaacatcgtc tttcggaccg
     7201 cggacgggac gctcggctgg cgcgacatgg ccccgttcga ccgcatcctc atcacggccg
     7261 gcgcgccgca cctgccgacc ttcctcgagg agcagctcca cttaggcggg atcgcggtgg
     7321 tgccggtggg gcagagcgag aatcagtccc tcatcaaagc gaccttgacc ccggaggggc
     7381 tcacccggcg cgtcctttgc ggctgcacgt tcgtgccttt gatcggccgt gaaggctggg
     7441 ccgcgggcga ggtcggttga ccctccagcg ctctcgctga tagaatcccg cgcctgggaa
     7501 agaattccat cgatgcggtt tgcggttgag gatcggggcg tggctcagcc tggtagagca
     7561 cctggttcgg gaccaggggg tcggaggttc aaatcctctc gccccgacca tgacccaccg
     7621 catcggttcg acccatgagc gcatgcgctt tcgggtcccc gtcgtgacgg agacccagaa
     7681 agcgcgcctc gaggcccggg tgacggggcg ggtccagggt gtcgggtacc gctggttcgt
     7741 ccaggccgag gcggaccggc tcagcctcgg cgggtcggtg cggactctgc cggacggggc
     7801 agtggcggtg gcggcggaag gtccccggcc caagcttctc gttcttttga agcgacttca
     7861 cgaaggtccc ccggcggccc gtgtcgaacg ggtggacgcc gagtggctca ccccccacgg
     7921 tcaaaccgaa tttcgaatcc tgtccggttg agcgaaccca tgcccgagaa tccacgccga
     7981 ttcgtatccg agctgtccga ggccatgcgc gcgggcctcg aacgtggcgc gctcacggac
     8041 gacgatctcg atccgctcct ccactcctcc gacttcgacg cgggcgcatt cgacctcttc
     8101 ctggccgagg cgcgccgtca gggcatccgg cttcccgacg aagcgaagcc cgacaatctc
     8161 aaccctcccg acagcgacca cacgatcggc agcctggagc ggcgctactt gaaggagatt
     8221 cagcgctacc cgatcctcgc ccgcgacgag gagcgacggc tctgggaagc gatgcgggtg
     8281 gggaaccagg atgcacgcaa agcgctgatt ctcgcctacc tcaggctcgt cgtttcgatc
     8341 gcgcgcgcgt accggaaccg aggcgtcgag ttcctcgatc tcgtcgagga ggggaacctg
     8401 ggcctcatca cggccgtcga tcgcttcgac gtggatcgcg gcatccattt cagcacgtac
     8461 ggcacctggt ggatccggca ggcgctcgcg cgcggcgtgg cgaaccagtc ccgcacggtg
     8521 cgcattccga tccacgtgct ccagatgatg cgccggttcg tggccacgca gcgccggctc
     8581 gagtccgagc tcaggcgcaa gcccgaggcg agcgaagtcg cgcaggagat ggggttgccc
     8641 gaggctcgcg tctaccggct cgagagcctg gtgcagtcga tccacacgct cgacgtggac
     8701 atgagcaacg aggcgttcca cggcctcatc gagtcggagt ccatcgagca gccgccgagc
     8761 ctcgacgaga tcgtcgagat gcagatccgc gatcagcagc tgaacgagat gctgaagcgg
     8821 ctctcagggc gcgaggaggc gatcctccgt ctgcgctacg gcttctacga cgaccgcccg
     8881 cgcacgctcg cggagacggg ggagcatttc gggctctcgc gcgagcgcat ccgccagctc
     8941 gaggagcgcg cgctcctcaa gctgcgccac ctgctcgagg ccgagaccag cgaggccagg
     9001 gcctcggtcc actagttccg gcggggctcc gcgagcgaga cccggatacg ggtgattcga
     9061 ggctcgagcg cccgcatggc aggtgacgtg gacgaaatgt cgcgatcccc ctgcacctgc
     9121 gcgaagcttg gatcctgatc cttcccaggc gcgggctcca ggtagagcca tgcccgcccc
     9181 ttgaggacgc tggacaaggc gcctccttcc cagtccgtca ccgcacccga gtaatcccat
     9241 ccgaatccgg ccatggtgaa cgggtgtccg ttcagccgct cgagctcggc gagcgtagtt
     9301 ccgagcgtga tgcctcgtgg aagggtccat cgactccggg taccgcgcag gatcgcgtgc
     9361 atggggtagc gtcgggcggt cacgttcatc cacacgatct cgacgcgctt ctcagggtcc
     9421 tccgggtaga gaagggtacc ggagagagcc tcgccctcac ccagcgacac ggtcacgtgg
     9481 cggacgttct gcgcgccata ccccgcgcca agctcgcgct ccgaatcgga cagcttcagc
     9541 acgcccagtc gctcaccggg gacgatctcc caggcggtct tcgggttctg cgcgcagccc
     9601 ggcagtgcgg ccgcgatggc caaggcgatg atgatcgctc tcactggcgg aagctgaaac
     9661 cgaggaatgc ctcgaaatcg ggagcgacgt ccgtgatgcc aaggcggacg ccgccgtcga
     9721 gctgggtgtt cggggtgatc gcgagggtcg cacccgtgtt gaagaggccg agccactcgc
     9781 cctcgtcggg cacgcggaac gtggccgcaa attcgacgaa cccaccgagc cctcccacga
     9841 ggtcgtggct agcggtcgcc gtagtcagcc actcggtcca ccgctctccc tggacgtcga
     9901 accagtccac ctcgcccatg acgcctaagc cgaagccggc gggaccttcg atcgcaagag
     9961 gtgcgatgag ccctccctcc agtgcgctgc cgggagagtc gtgagatgtc gggatgtcga
    10021 ggaagggcat gagcgcgaac gcggtcttgc cgccgtcgtt gccccagaaa ttccacttca
    10081 agcggatttg aaggtcgcca ggccccgtgt tgtcggagac atcgatctcg cctggatgga
    10141 gcgtccggtc gaactcgaaa tcggtgacac ggaagagctg ggacgtcccg aactgaatat
    10201 cggcgttccg atggatgccg cgcttgatca agaaccccgc caagcccgtc gacgtcactt
    10261 cgaccccatc ggacgtctcg cttccatggc tgacggcctc gatctcgacc tgcgtatgtc
    10321 ctttctcgac ggtatagggg ctctcggtca gatccggacg gtccgtactc atctcgcgtg
    10381 tgaagcggga ttcggagctg tcggtggagc ctccggtttc ggtctgctcg gccaacgagt
    10441 ggaccggcag ggacgaggtc ccacatatga aagcgaacgc gatcgcgcga acggtcacgg
    10501 cttcctcctg aaacgagtca aggggcgcac caaccggcat cataacggcg ccccggaccg
    10561 ggaggtacaa tggagtttcg gaggggaggg gctatgaaac gttacgggtt cgtcgtgtgg
    10621 atcgtgtgcg cgaccgcgct aggctgtgcc ggcaccgcgg gtcacctggc caagccgggg
    10681 aaggaaatca aggcgcacgg tctccttgcc gccggagcgg agtgccccat gctcgagacg
    10741 agcgacggtc atcgctatgc gctggccggt agcctggaga gcttcaagac aggcgatcgc
    10801 gtgtgtgtac gaggcaaggt cgccggagcc tcgatctgca tggccggcga ggcgaccctg
    10861 acgatcgaat ccatcgaagc tgaaagcgcc tgccgctagg agcggcattc acccgcggcc
    10921 tttggccgcc agaacaaggg aggaagacgg catgctgctc gttcgcgaga tcatgtactg
    10981 caagccaggc aaggtgaagg cgttcgtcga gaagaccctg gcgatgcaga agctcatgaa
    11041 gaagttgccg aaccagccat ccatgcgggt catgacggac ttctgtggcg agcaattctg
    11101 gatgtgcgtg atgcagatgg aagttcccga catggcgacg ttcgagaagc tgatgagcgg
    11161 ggaaggggag agtcaggaat cgatgaagga gttcgagaac atcatgaagg gctaccacga
    11221 cctcgtggat cacggacggc gggagatcta caagatcgag ggttaggtcg cttcttgcga
    11281 acgagcggag gggccgccgt ggtcgggcgg ccccgctctt cgtcctaggt tagtgccgct
    11341 tgaagtactg cacggctcgc tcgtgcttct ccgccgcttg gcgcgaggag aaggtgccga
    11401 ggttgcgccg cctgccggtc ttcgggttct tcttgatcga gtacaggcgg tattgaccgc
    11461 tcttcaattt gcgtatcatg gctcctcctg ccgccggtag acacccggcg tctccttcta
    11521 atgctggacc ggcggggagc cagggctcaa gggtggagac ggacctgcca gtctcgccga
    11581 ggcgccggcg gcccagtctt ggaggtacag atgaagaagt cggagtcagc ttcgacattg
    11641 atcgacggga agatcaagga gctcggcgat tggcgtggaa agaccctggc gaaggtgcgc
    11701 aagctcatcc atcaggccga ccccgagatc gtcgaggagt ggaagtggat ggggaccccc
    11761 gtcttctccc acgacggcat cgtctgcacg ggagagacct acaagagcgt ggtcaagctg
    11821 accttcgcca aaggagcatc gctggacgac cccgccggcc tcttcaactc gagcctcgac
    11881 ggaaacgttc ggcgcgcgat cgatattcgt gaaggcgaag ccatcgatga ggcggcgctc
    11941 aagaatctca ttcgcgccgc ggtgacgctg aacctcgagg ggaagagcaa gccgaagtcg
    12001 cggcccaaaa cggctacggc gaaggcgacg aggaagaagg cgaagaggtc gagcacggcc
    12061 tgatccgact gacgctacag gttgaaggat atccccgttc gaagcacggg gacgcgctcg
    12121 tagacgtcgg ggagatcgcg atagagccgg tccgccgtat agagccagta gtccgcaccg
    12181 atggaaaatc ctcgagtcaa cggcacgtcg aaccgaaccc gcgacgtact gatcaggtgg
    12241 tcggagaccg agccgttgat ggagtggatc caaaatctag agtgcgtcag catcaggtaa
    12301 gggtggccat ggtagtagaa cgtacttccg aacttgaagg cgagcccggg tccgaagtcg
    12361 tacgaacggc ccgtgaaggt ctgatagttg tccttggtcg cccccatgat gagggcattc
    12421 atgtggagct gggtccgaac cgcgaaatgc tcgcctgccc gaaactgtga gtagagcgag
    12481 gcggcgagac tctgcccccc cagctcgata tcgctgttgt tgaagtaatc gaaatgctgg
    12541 gtgaagccga tcaagtgctt ggaatgatcc gttcccatga ccggcgacga cgccaggagc
    12601 ccgatcgtct gcacccgccc gatcgtctgc gcgtcattga tgttgatctg gaagttgaag
    12661 tcgaacgaat cgtacggctt cttgttgtcc ccgaggaacg ggtcgccgac ataggcggcg
    12721 acctcgatga agggcctggt cgtgtccgca tcccagagct ggtcttgccc cgaggtccgg
    12781 agcccgacac ggtacgagac acggctcgag cgcggaaaac ggtccggcgg gttgggcccg
    12841 acgcggttga attcccccgt gacgagccgc gtaaagccgc gcaccgggct gatgatcagc
    12901 ccgccgacct cgccccagct ccgccgggag ccggtggcgg tgttgtccgt caccatctgc
    12961 gataggcgga agaagacctc ccccagcgcc atgcccccca tgctcgtcag gatccagtcg
    13021 ttgatggccg gatggtggac ctcgccgaag tactcccaca tgaagctgcc gccccaggta
    13081 tagggcaccg agccccagta ggacatgccg ttcgatcggg cggcgttgaa gtaaaggtcg
    13141 ccgtggtagg gatgtgcgta catgttcgtc gagaagttgt tgtcatcgaa ctcgaacccg
    13201 ttctggatgt tctcctggaa cgagttgaac ccaatgcgaa agccgggatt ggttccgttc
    13261 tcgcggatgt agcggtcgta gctccagacg agagcgttca cccccaccac ctcgctcgcc
    13321 gcgagccagg gacgtttgac ggcgacgtaa ccctcatccg gcctctcgat ctgcttcgcc
    13381 gccgagctat cggggaactg cggcgccgcc ctctgcgcct ccgcccgcgc ggacacgacc
    13441 atgccgacga cgaaggccag gagtccgagg aggactcccg gtcgaatcgg tgcgccaggc
    13501 tcgaccagcg tccgtcgcat cagaagagcg ccatcccggc gaagaacagg ggctccgtct
    13561 cggcgggagc gtccaggcga tactcgtccc agatccgcgc cacaccgccc cccgcgaaac
    13621 ccttcgcctt cctgctgaag gtccgctcca ccatggcccg cgcctgcaag gagaagtgga
    13681 ggcgatcatt ttcgttggga tcgtccacct tttccggaat gtaatgggcg tatcccatgt
    13741 cgatgccgat gctggtacct cggcccgtcg ggatcgcata gccgtaattc caggtcagga
    13801 tgagcgtgct cgaaacatcc ccctggagat ccggaagccc cgctgtcagc atggagtaga
    13861 agcgtcgaac ggacgtgccg atgcccgtgt tgacggcgct gagattgctg ccgtacgtga
    13921 tccagtccac gtcgccgttg cccgcgatgt tgaccatccc gaacgggacg ccgtgttgtt
    13981 cctcggcgaa gttcagggca cccatctgga agcctcgggt cctcgtcgcg aaattgacca
    14041 ccccgatctg cgttccacgc atgtccagag cggcgttggc accgccgaac tggagcccga
    14101 ccatctggtc cgtgacgtag ttgaggccgg tggctacctg ccagccatcg aaggcacgcc
    14161 cgacgacgtt ggctatcccc gcgacctgga atccccgtgc gccgccatcg gcgagattga
    14221 agacgggcga gagctgcacg cccgacatgc cttcgccggc cacattggcg agtgcggcgg
    14281 cctcgaaacc gctgagacgc ccttgagcca cgttcgcgag tccgctcaga tgaattccac
    14341 tcacgtcctg catcgcgtag gagacgactc cggtcaggtg gacaccgctg gccttcccgt
    14401 aggtctgggc gtagatcccg gtgagctgaa ctccgcgagc gtttcctccg gtcatggtca
    14461 cgacaccgtg gagtccgagc gcatcgacgg aaccgacgcg accgtagagc agggagaggt
    14521 gaacggaggt cgcgacctcc gggctccggt tcgtggccag cggatagagg aacgcgagca
    14581 cgatcggact ccggccctcc gccgcgaggg cggacgccga gaggaagagc gctgcgagca
    14641 cgatatgagc catcaaaagg cgcggggcca tcagaagtac cagttcaatc cgagggtcat
    14701 cgggacgaac cacgccttat cgaagaagtc cttcccgact gtgtggtagt tggccgtgac
    14761 atcgagcgcg agcgccgcgg gcttggagat ccggagctcg tagccacctc cgaacatgaa
    14821 gcccgcgccg ctctcatcga cggtctcctc gtgatgggtc gagtccgctc cggtcggaac
    14881 cttcccaaac gcgacacgcg cgttcacgag cccggcaccg gcacggagat agatgcctcc
    14941 cgtcacgtgc ttcgggtcac ctgggaagac cgtgatcgca gcaccgaacg tctggaggct
    15001 gacgcgtacg aaggcgtcgg cattgcccac cacttgcagc cactggacct gctcataccc
    15061 gagcatcgca tggcggccca gcatgtggcc gagcctcagg cggggcgcgg aaccgtcttg
    15121 ccacggcgat tccagatgaa ggctgtcacc cgtggtgatg tgggctttgg accagccgta
    15181 gccgatgccc gccacccagc cggtacgaac gtgctcgtag agagcaaacg caggctgcgc
    15241 cgtgaacgtg gggaggaaca ggatgagcaa gaatgcggtt cggcggagcg tgactaccgg
    15301 gctctggacg gcgtgtaacg ccacgctcag aagtagtacc ggagcgagaa ggcggcgccc
    15361 ccggcccaga ccgagtcggc ctcccggttc tcccggtcga ggacgcccgt gatgtccagc
    15421 gcgatcagga aatcgggctt ccccgtgtac gcgattcgga aaagtgtctg gcgcacactt
    15481 ccattgtctg tcgtggtcag ggccggctgc gaggtctggc gatacgcgag cacgaggccg
    15541 atcggaactc cccacgcggc gcctccgtcg aggtccacgc tcgcaccgaa gccgttctcc
    15601 caggagtcca cctggtcccg cctcggagat tccccgtagg aaccttcggc tagagccgtg
    15661 aggccgaagg tgcggctcat cgcccaggcg aagcgcagcc ccgcgctgga tcggaccgtc
    15721 ggaatgttgt cgatcaggga cggatctgga attccgtcga tcacatcctc ggtgaattgc
    15781 ctgatgtcga tgagggtgag ggtctgcctc gtgacgtcca gcgacccgac aagactggtc
    15841 ctgggggttt cgcggattcg cacgagccac ccgagctcga tcccgctcgt gacgttcacg
    15901 ccttcgttga ccagggagga cacttgcgtg ccgatgcgcg tgcgcatgcc cagtgcggct
    15961 cgaactgcga tccagtcccg gatcgcatgc tggtactcca tgcccaggag ggcgtacgcg
    16021 agggttccgt tcagcgtgag aagcgtgtcg ccgcccacca cgatcggggg gtaattgaac
    16081 tccggggtca tcgtgtagcc cagtgaattc cgaacgtagg tacgcacgaa cgcgtccgga
    16141 acgaggtcgg tggagacgaa ggtgtgcccg ccgagcgcgg gacggggccc ctgttgcgcc
    16201 gcagccggcg ccgccagcgc aagggtcccg gcgatggcca gagcccaggg accgaatcgg
    16261 acgcgagcgc gtggcatcat cgggctccga ggggcaaaag gatccccacc atgagctgca
    16321 agccactgga gtggaatcga tcgggcagct cctgaaccag gccctgctcc gcgtcacgaa
    16381 ggttgacgag tccctgcacg tagcggagct ccgtggtgag gcgcgaacga cccgccggga
    16441 agacgacacc gaagccgaac agcgcgccga cgtcgacgct gtggaggagc ggctcgatgt
    16501 cctcgtcgat cccttcgccc gacacccgag cggagttgag gaaccctacg tccatcccgc
    16561 ccgcgaagta ggtccggcca tgcgcggctc cgaatttgac cacgaccggg accgagagat
    16621 aatccaagtc gaggtcgaga gagcgctcgt cgcccgggtc ggcatccgcg gatcgaagct
    16681 tcgtgtttcc ctgaacgaac gt
//
LOCUS       DASTKC010000043         6961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_503906, whole genome shotgun sequence.
ACCESSION   DASTKC010000043 DASTKC010000000
VERSION     DASTKC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503906"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFR25_01860"
     CDS             <1..240
                     /locus_tag="VFR25_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939824.1"
                     /translation="IEREADYGTSSRQAPAAAAVTGGDARTVTVPIDLGSLPSGRVRL
                     VFEVRIEVDAETSGGRAKKSTRGGAMMRDLETIPD"
     gene            342..1364
                     /locus_tag="VFR25_01865"
     CDS             342..1364
                     /locus_tag="VFR25_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9939825.1"
                     /translation="MDRALPLSLRDSALTGSESREESLALAETLVHSTADPEQVREAA
                     RIFAAQDRAERAIETLRQGILTHPRQADLLVLLGDLLSRSGAHDEAGLYLRQAVELEP
                     QSADASFRLGLHHARRGHSEAARASYETTVRLDPAHVRAWVNLGLTRSDLGERKGAID
                     ALRRAVNLDPACSDARSNLGTLYAEWGMAEEALEEFRRAVELSPQSAEAHFNLGWALL
                     ADDELQEAEAMLEASARLDPASAEALYALAILHLRIGAYARAVGELKRALEIRPDDAR
                     LHYHLGVAYNNQDAADQAILALENAARLRPEDPRIHRLLGVAYDKKELPLRARDAYRR
                     AAALNG"
     gene            1367..2161
                     /gene="trpA"
                     /locus_tag="VFR25_01870"
     CDS             1367..2161
                     /gene="trpA"
                     /locus_tag="VFR25_01870"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET9939826.1"
                     /translation="MPGRIGRALERTAAEGRLAFVPFLTSGFPDPPESDRLLAALCEE
                     GADLLELGVPFSDPLADGPVIQESTRVALAKGVRLADTLEQARRLRARFETPLVVMTY
                     LNPVLAYGAERFAKDAVAAGVDGLILVDLPPEEDPALWSSFQSAGLDTIALVAPTTDP
                     TRLSSIAAAARGYLYVVARLGVTGGGAGDARLDDLLRACREHSPLPRCVGFGWTPTSD
                     VAPLRGHAEGVIVGSALIRELSEEKDGARREERLRRFARAMRARLS"
     gene            complement(2196..3188)
                     /locus_tag="VFR25_01875"
     CDS             complement(2196..3188)
                     /locus_tag="VFR25_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939827.1"
                     /translation="MLAVVLSVVLGCGSEDTIKPGTDPDAVVWTKLTTFPRFTTQPDS
                     MQLSDATALYPDWRGDSVVFNALDPVTRHWARIAYLDVRDPASVYVSNYPGLATWHDL
                     GPRWVAPGLVIFASGTLSNPVQNPEKYDLYYRIVDTGVNRRLFTWTAAERYPDPQPGG
                     VGLVYVEGDNNLRGRLVFVADTANTANRTYMTAPDVYAGEAQWDPTGAKVVFSADSTV
                     QFGARHIWWVQPGDLSSHQLTFGPYVDQFPTFSPDGTKILFVSNRSGRSSLWIMDPVA
                     GEAGGLKRIAFEDVGARIESPTWSDDGTRIIVTSNGRDYVRQLWMLSNLDLTLP"
     gene            3568..3858
                     /gene="csrA"
                     /locus_tag="VFR25_01880"
     CDS             3568..3858
                     /gene="csrA"
                     /locus_tag="VFR25_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HET9939828.1"
                     /translation="MLLLTRKLGENIRIGDDVKITIVEVKGNHVKLGIDAPPSVKVHR
                     EEIYERIQQENRRAQALRDAGGTPNGETGTARPSGAQGNPPSRPENGRESKP"
     gene            3892..4230
                     /locus_tag="VFR25_01885"
     CDS             3892..4230
                     /locus_tag="VFR25_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939829.1"
                     /translation="MSTAYGGADPGELDRWIERGEEVLALFDCALRSCQEENRSIALR
                     TVLDLMGRLEHRSRDVGGRVLAHFDACLSDIERGDFEHAFEGLLDLRESWMEAMATLK
                     KDRDRAASLN"
     gene            4256..4408
                     /locus_tag="VFR25_01890"
     CDS             4256..4408
                     /locus_tag="VFR25_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939830.1"
                     /translation="MAQLPSVERARRRIHRRYYDRPEVRRTLAGLILRRMASSPKRKE
                     SPTETA"
     gene            4607..5803
                     /locus_tag="VFR25_01895"
     CDS             4607..5803
                     /locus_tag="VFR25_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9939831.1"
                     /translation="MRSRARLPAPPPDPPPDDRVLDAAWSHYRHAPQGVLAVDRDGKV
                     LAVNPAAARLLGFEPDPLEGEDAARVFRAPKGEGHVPPGACASAEEPREVEVVTRSGD
                     VLPVSLKLLPLEGRDGSLRGTLALFQDLREQKAREEQWRRRDRLASIGELAAGVAHEI
                     RNPLAGISNSAQLLKRRLESDDPRSQFAHLILEEVSRLDRIVESLLQFARPSTPKLTR
                     QSILPSIDRALMLVHEVAVQENVNLRVERADRVPEIYMDHDQVLQVVLNVLMNALQAL
                     TQGGEIEVKVGPARKRAAERSSIGRRATDRLLERERPPLQDVVEVRVKDNGPGIPAAV
                     IARVFDPFYTTRTQGTGLGLSICQSIVREHGGAISIESTVGQGTTVIIDLPVEKRHGD
                     RRGSPR"
     gene            5775..>6961
                     /locus_tag="VFR25_01900"
     CDS             5775..>6961
                     /locus_tag="VFR25_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9939832.1"
                     /translation="METDAAVLAKQTVGILVVDDERTLRFTLKEGLSDEGYRVETAAD
                     GAEALEKLEREEYQLVLLDQKLPDTNGLELLKTIKARKRGVQVVMMTAYGQIPQAVEA
                     TKAGCYEYLSKPFELDHLKLIVRNALAQTRLTEEVARLRDVDRRRSGSTLIVGGGSRM
                     NKVIEIVAKMAQSPTSTVLLQGETGVGKELLAREIHDRSARRDGPFVELNCSAVAENL
                     LESELFGHEAGAFTDAKKAKKGLMEMANLGTLFLDEIGEMSPNLQAKLLRALEMKRFR
                     RVGGTVDIAVDTRIVAATNRDLKAMVGQERFREDLYFRLNALQLVIPPLRERPEDIPP
                     LIDHFVGHWNRELGRNVKGPSDEALTLLLAYRWPGNVRELKNVIERAILLESEEWILP
                     EHLP"
BASE COUNT         1190 a         2348 c         2402 g         1021 t
ORIGIN      
        1 atcgagcgtg aggccgacta cggaacctcg tcgcgccagg cgccggccgc cgcggcggtg
       61 acgggtgggg acgcgcgcac ggtcacggtg ccgatcgacc tgggctcgct gccgtccgga
      121 cgcgtacgcc tggtgttcga agtccgcatc gaggtcgacg cggagacctc cggggggcgc
      181 gcgaagaagt cgacgcgcgg cggcgcgatg atgcgcgatc tggagacaat tcccgactga
      241 cgcggcgcgc gccgcgggcg cgcacaaaaa aaaatgcatc gggcccgtca attcgggcct
      301 tttttgtttg actcattcgc gctcgtggat agagtgcgaa cgtggatcga gcgctacctc
      361 tctcccttcg cgacagcgcg ctcacgggat ccgaatcccg cgaggagagc ctcgcgctcg
      421 ccgagacgct cgtgcactcc accgcggatc cggagcaggt gcgcgaagcg gcccgcatct
      481 tcgccgcgca ggaccgcgcc gagcgcgcca tcgagacgct gcgccagggc atcctcacgc
      541 atccccgcca ggcggacctc ctcgtgctcc tgggggatct cctgtcgcgc tcgggcgcgc
      601 acgacgaggc cgggctctac ctgcggcagg cggtggagct cgagccccag agcgcggacg
      661 catccttccg cctgggcctc catcacgcgc ggcgcggcca ctccgaagcg gcccgcgcct
      721 cctatgagac aacggtgcgg ctcgacccag cccacgtgcg ggcctgggtc aatctcggcc
      781 tgacccgctc ggatctggga gagcggaagg gcgccatcga cgcgctccgt cgtgccgtga
      841 acctcgaccc cgcctgctcg gacgcgcggt ccaatctcgg caccctctac gccgagtggg
      901 gcatggccga ggaggcgctc gaggagttcc gccgcgcggt cgagctgagc ccccagagcg
      961 ccgaggccca cttcaatctg ggatgggcgc tcctcgccga cgacgagctc caggaggcgg
     1021 aagccatgct cgaggcgtcc gcccggctcg atccggcgag cgccgaggcg ctctacgcgc
     1081 tcgcgatcct ccatctgcgc atcggcgcct acgcgcgcgc cgtgggcgag ctgaagcgtg
     1141 cgctcgagat ccgtcccgac gacgcgcggc tccactacca cctcggcgtc gcctacaaca
     1201 atcaggacgc cgccgatcaa gccatcctcg ccctggagaa cgccgcccgg ctgcgccccg
     1261 aggatccgcg catccatcgc ctgctggggg tggcctacga caagaaggag cttcctctcc
     1321 gcgcccgcga cgcctaccgg cgcgcggcgg ccttgaacgg ctgaccgtgc ccggccggat
     1381 cggccgcgcg ctcgagcgca cggccgccga gggtcgcctc gccttcgttc cgttcctcac
     1441 gtcgggattc cccgatcctc ccgaatccga tcgcctgctc gccgcgctct gcgaggaggg
     1501 ggcggatctc ctcgagctgg gagtgccctt cagcgatccc ctggccgatg gcccggtgat
     1561 ccaggaatcc acgcgcgttg cgctcgcgaa gggggtgcgt ctggcggaca cgctggagca
     1621 ggcgcgccgc ttgagagcga gattcgagac accgctcgtc gtgatgacct acctgaaccc
     1681 ggtcctcgcc tatggcgcgg agcgcttcgc caaggacgcc gtcgcggcgg gagtggacgg
     1741 gttgatcctg gttgatctgc cccccgagga agatcccgcg ctctggtcct cgttccagag
     1801 cgcggggctc gacacgatcg cgctcgtcgc tccgaccacc gatcccaccc gtctctcctc
     1861 gatcgccgcg gcggcgcgag ggtatctcta tgtcgtcgcg cgcctcggcg tgaccggcgg
     1921 cggggcaggc gacgcacggc tcgacgatct gctccgggcc tgccgggagc actcgccgct
     1981 tccgcgatgc gtgggattcg gatggacccc gacgagcgac gtcgcgccgc ttcgaggtca
     2041 tgcggaaggg gtgatcgtgg gaagtgccct catccgggag ctctcggagg agaaggacgg
     2101 cgcccgccgc gaggagcggc tgcgacggtt tgcgcgggcg atgcgggcgc ggctgagcta
     2161 ggaacgctta cgacgcgaac gtcgtggcgt gaagctcaag gcagcgtgag gtcgaggttc
     2221 gaaagcatcc agagttggcg cacgtagtca cgaccgttgg acgtgacgat gatccgcgtg
     2281 ccgtcgtcgg accacgtggg tgattcgatc ctcgctccca catcttcaaa ggcgatccgc
     2341 ttcaaaccac ccgcctcacc cgccacgggg tccatgatcc agaggctgga gcgcccggag
     2401 cggttcgaga cgaagaggat cttcgtaccg tcgggactga atgtaggaaa ttggtcgacg
     2461 tagggtccaa aggtcagctg gtgcgagctc agatcgcccg gctgaaccca ccaaatgtgc
     2521 ctcgctccga attgcacggt gctgtcggca ctgaagacga ccttggcccc cgtgggatcc
     2581 cattgggcct cgccggcgta gacgtcgggg gcggtcatgt acgtccgatt cgcggtgttc
     2641 gccgtgtcgg ccacgaaaac cagtcgtccg cgcaggttgt tgtctccctc cacgtaaacc
     2701 agaccgactc cgcctggctg cggatcgggg taacgctcgg cggcggtcca cgtgaagagc
     2761 cgacgattca ccccagtgtc cacgatccgg taatagagat cgtatttctc ggggttctgt
     2821 acagggttcg ataaggtccc ggaagcgaag atgaccagcc cgggcgcgac ccagcgaggg
     2881 cccaagtcgt gccatgtggc aagtccggga taattggaga cgtagacgga cgcaggatcc
     2941 cggacatcga gataggcaat gcgggcccag tgccgcgtga ccggatcgag agcgttgaag
     3001 acgaccgaat ccccgcgcca gtcggggtac agcgcggtcg catcgctgag ctgcatggaa
     3061 tcaggctgag tggtgaatct cgggaaggtg gtgagcttcg tccagacgac ggcgtcgggg
     3121 tccgtgcccg gcttgatcgt gtcctccgag ccgcagccga gaacgaccga gaggacgacg
     3181 gcgagcaagg aaacgaggaa accgcgggtt cggggccggc cgtcaggtac gcgggatctg
     3241 tccaggggag tcacgtggcg gaatggtagc aggggccgtg cctcctctca agctgatgcc
     3301 ccggccgaag ccctcctcgg aatagcgcgt tagcacccga tttcgcgcgc gggtttcacc
     3361 acccgacacc accgtttcac ggcgcgatct ggctcgatcg aaccgcgcca agaccttcgt
     3421 tcttccagtg ccggcgcggg gattcgccat tcagatggcg cccgctcctg ccgatggttg
     3481 ggccggaagg gaaaggctcc gggaccaacc cggcgccccc tccgcgaccc agaccccggc
     3541 aggacgctgg agaggaacga gggcacgatg ctcctcctga caaggaagct cggagaaaac
     3601 atccgcatcg gtgacgacgt caagatcacc atcgtggagg tgaagggaaa ccacgtgaag
     3661 ctgggcatcg acgccccgcc gagcgtgaag gtgcacaggg aagagatcta cgagcggatt
     3721 cagcaggaga atcgtcgcgc ccaggctctt cgcgacgccg gcggcacccc gaatggcgag
     3781 accggcacgg cgcgcccgag cggcgcgcag gggaatcccc cgtcgcgccc cgagaacgga
     3841 cgggagtcga agccttgacc ggccgcatcg acacggcggg ggcctgacgc gatgagcacg
     3901 gcctacggag gggccgatcc cggcgagctg gaccgctgga tcgagcgcgg cgaggaggtt
     3961 ctcgcgctct tcgactgcgc gctacggagc tgccaggagg agaaccgctc gatcgcgctc
     4021 aggacggtcc tcgatctcat ggggcgcctc gagcatcgat cgcgcgacgt cggaggacgc
     4081 gtcctggcgc acttcgacgc ctgtttgagc gacatcgaac gtggagactt cgaacatgcg
     4141 ttcgaagggc tcctggatct cagggagagc tggatggaag caatggcgac cttgaagaag
     4201 gatcgggacc gtgccgcaag cctcaactaa gccggcccag atggaggagc ccgacgtggc
     4261 ccagctgccc agcgtggagc gcgcgcggcg ccgcatccat cgccgctact acgaccgccc
     4321 cgaggtgcga cgcacgctcg cggggctgat ccttcgccgc atggcttcct ccccgaaacg
     4381 caaggagagt ccgaccgaaa ccgcttgacc cccggcgagg ggcactggta aagatgtgac
     4441 cactcacgat cggttctgca ctcgcgagtt cgggcacagg gatgatgccg gaggtcggct
     4501 tggaagccga aaagggaagg gcgcccggaa cgcttcacgt tcgcaccgcc attccattcc
     4561 cccgatccct gcccggcgcg cctcctcgaa gcgcgggcgg gacgagttga gatcccgcgc
     4621 ccgcctcccc gcgccgcctc ccgacccgcc acccgacgac cgcgtgctcg atgccgcgtg
     4681 gagccattac cgccacgctc cgcaaggcgt gctcgccgtg gatcgggacg gcaaggtcct
     4741 ggcggtcaat ccggccgcgg cccggcttct cgggttcgag cccgatccgc tcgagggtga
     4801 ggacgccgcc cgcgtcttcc gtgctccgaa gggcgagggg catgtgcccc ccggcgcctg
     4861 cgcgtcggcg gaagagccgc gcgaagtcga ggtggtgacg cgctccggcg acgtcctccc
     4921 cgtgtcgctc aagctccttc cgctcgaggg acgggacggg tcgctccgcg gcacgctggc
     4981 gctcttccag gatctccgcg agcagaaggc gcgcgaggag cagtggcggc ggcgcgaccg
     5041 gctcgcgtcg atcggcgagc tcgcggccgg tgtcgcgcac gagatccgga acccgctcgc
     5101 cgggatcagc aactccgccc aattactaaa gaggcgcctg gagagcgacg atccgcggag
     5161 ccagttcgcg cacctgatcc tcgaggaggt ctcgcgcctc gaccggatcg tcgagagcct
     5221 tctccagttc gcgcgcccgt cgacgcccaa gctcacgcgg cagtcgatcc tcccttcgat
     5281 cgaccgcgcg ctcatgctgg tgcacgaggt ggcggtgcag gagaacgtga acctgcgcgt
     5341 cgagcgcgcc gaccgcgtgc cggagatcta catggaccac gaccaggtcc tccaggtcgt
     5401 gctgaacgtc ctcatgaacg cgctccaggc gctcacgcag gggggcgaga tcgaggtcaa
     5461 ggtcggaccg gcccggaagc gtgccgcgga gcggagctcg atcgggcgcc gcgccaccga
     5521 ccgcctgctc gagcgcgagc ggcctccgct ccaggacgtg gtcgaggtgc gcgtgaagga
     5581 caacggcccg gggatcccgg cggccgtcat cgcgcgggtg ttcgatccct tctacacgac
     5641 gcgcacgcag ggcaccggtt tgggactcag catctgccag agcatcgttc gcgagcacgg
     5701 cggcgccatc tccatcgaaa gcacggtggg gcagggcacg accgtgatca tcgatctacc
     5761 cgtggagaaa cgacatggag acagacgcgg cagtcctcgc taagcagacg gttggaatcc
     5821 tcgtggtgga tgacgagcgg acccttcggt tcaccctcaa ggaaggcctg tccgacgagg
     5881 ggtaccgggt ggagaccgcg gcggacgggg cggaggcgct cgagaagctc gagcgcgagg
     5941 agtaccagct cgtgctgctc gatcagaagc tccccgacac gaacgggctc gagctcctga
     6001 agacgatcaa ggcgaggaag cgcggcgtcc aggtggtgat gatgaccgcg tacgggcaga
     6061 tcccgcaggc ggtcgaggcg acgaaggccg gctgctacga gtacctctcg aagcccttcg
     6121 agctggacca cctgaagctg atcgtgcgga acgcgctcgc gcagacacgc ctcaccgagg
     6181 aggtggcccg gctccgcgac gtcgaccggc gccgctcagg ctcgaccctc atcgtcggcg
     6241 gcggctcgcg catgaacaag gtcatcgaga tcgtggcgaa gatggcgcag agcccgacct
     6301 cgacggtgct cctacagggt gagacgggcg tggggaagga gctcctcgcg cgcgagatcc
     6361 acgaccggag cgcgcggcgg gacggcccct tcgtcgagct caactgcagc gccgtcgccg
     6421 agaatctcct cgagagcgag ctcttcggac atgaagcggg cgccttcacc gacgccaaga
     6481 aggcgaagaa ggggctcatg gagatggcca atctcggcac gctcttcctg gacgagatcg
     6541 gcgagatgag cccgaacctc caggcgaagc tcctccgcgc gctcgagatg aagcggttcc
     6601 ggcgcgtggg gggcaccgtc gacattgctg tggacacgcg catcgtcgcc gcaaccaatc
     6661 gcgatctcaa ggcgatggtg ggacaggagc gcttccgcga ggatctctac ttccgtttga
     6721 acgcgctcca gctcgtcatc ccgccgctcc gcgagcggcc ggaggacatt ccgccgctca
     6781 tcgaccactt cgtcgggcac tggaaccgcg agcttggacg gaacgtgaaa ggcccgagcg
     6841 acgaggccct cacgctgctg ctcgcctacc gctggcccgg caacgtgcgc gagctcaaga
     6901 acgtgatcga gcgggccatc ctgctcgaga gcgaggagtg gatcctgccc gagcacctgc
     6961 c
//
LOCUS       DASTKC010000044        14195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_741214, whole genome shotgun sequence.
ACCESSION   DASTKC010000044 DASTKC010000000
VERSION     DASTKC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741214"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..2007
                     /locus_tag="VFR25_01905"
     CDS             151..2007
                     /locus_tag="VFR25_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9939833.1"
                     /translation="MDRSLAIKKRAQALVQKGQIDAALAEFEKLFETGDRDPYDFITV
                     ADLLAKRGAMQEAVRRYRQAIEEYGKAELYKNAIAVCKKILRISKEDLQIHRSLGELY
                     GKEGLYGDAQIHYLEFAEGSIRRHEFEAALSAVEQVVLLSPDNDDLSEKLVSIAMKAD
                     LPERGGKELLRRAERMEQRGLMSQGAELRERASTLAPGLVAESASAAAESSPEMPRAQ
                     AHAGQVELERSAPAMPYNPSPPTGFGDAPPAEAAPPLELESANGSRYEGARFTPPAPA
                     AGPPAAAPPAAAPPAAAPPTPAAEAPVAASSTAEIPAGPDRHEAMAGEHLRAGQIEPA
                     AEEFWKGAELAFFRSDLARARALLESLLQIRPNHEPALRRMVDITSQADDTAAEAKAR
                     FELGELYLGQQEWGLARAEYMRALELDSSNDRARMRVARIDAMNGEVTGSPVDLDNLP
                     HQKPAASVRVRDDQPAPTDSLVDLEEIIDEFKAGVSSTISGEDHESHYDLGMAYMEMG
                     LYDEAVSEFQIASKGSAIELKCLEMIALCYLEKNEPATAAKELKRALELPGHGPDETI
                     SIRYNLGMALERLGNLDKALQYFEEVYLLNVDFFKVATKVRELKQRIAAGGV"
     gene            2007..2855
                     /locus_tag="VFR25_01910"
     CDS             2007..2855
                     /locus_tag="VFR25_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9939834.1"
                     /translation="MPASIVDLHMHSTASDGSLAPADVVALAQRNGVKIIALTDHDSV
                     DGLPAALERAREAGIRIIPGIELSVSEQNMDVHLLAYGFDTTDARMLEAIARYRESRH
                     ERARKILTRLKGLGIRIPMEEIEEIARGGAIGRPHVAEALMTNGHVETFHEAFQRYLG
                     HHAPAYVAKHTVSLEEAVEVVRDAGGVTVLAHPGTLNRDAWIPGLARRGLDGIEVWHS
                     KHGPAEINRYREVARAHGLLMTGGSDFHGERTPDVSIGSVAVPEQVIADLDEALRARR
                     ALKAHP"
     gene            2934..3488
                     /locus_tag="VFR25_01915"
     CDS             2934..3488
                     /locus_tag="VFR25_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939835.1"
                     /translation="MRTVVRSAVLIAFSVACGYAKFLLFPYLFFVEIFIVSVFLSGSL
                     VGVAWGAWVGAVAGAVFSLVNPYGVPHPWVMAAQVLSDALVGAAGGIAGRWLLHGSGE
                     TRLGARSRTALLLGAGIVLTAIYDLLTNTAQGIAYGSIPAALALAVVPSLQHIASNAA
                     IFAIVGNLAFPWLARHPAVVRHAA"
     gene            3478..5637
                     /locus_tag="VFR25_01920"
     CDS             3478..5637
                     /locus_tag="VFR25_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939836.1"
                     /translation="MRPELRPVVLMAGLVCALAAAPRARAQSTAPRDSTSHAIAPVDS
                     TRHSVSAAPHDSLALRAARRDSLRAVAAVRDSIRFARALQDSARSVLARRDSIWADRA
                     GIQFEEQRKRGEITLEHAWVGRRGAILRGLPLAGIPVGTLSVPDAGSPIRTEPPGSEG
                     IAATDRPLVSAMPFGVGIVDLPVALDSPRAEGDEPLDLVEVEAVPAPGSYRRAGELLA
                     DPAAEQVFVHAMPGTDAHYRRARSALYYGNGDEGELDTAARFASATFGWGIAGSYARH
                     EADGLAPLHHAKATRYAVASGLPRALHHTLWIEARAMEWNIEDEAIGIDPINGTPFSA
                     VGRAEISRRDVLLHGQAGGSNASSVWTAQLEELKRTRVEGTGARQRWIFPGVQATWDG
                     HVDLDRQWSVLAHAEGSSRRIRYDENAAPIVDGRREEGRLGLALAHADAGGGSRLDVA
                     YDARETAPSFADARASLWTERGRMRGRIDLERSHERPSWVDLATPASTTERLDPTFTQ
                     RLILTRSGDPSLEPRRLTGGLARGALELGPNLAVTLEGSARYVEDDFGWTLTRVATPE
                     TLLVDTRAAVRGSGWVSYLGSGFRARTGSARWRAFGWVRGGPDDLGPRSGLVPRMGAD
                     VAADFRVTFFEGDLPLELGVRGHALGPSHGTIEAAGSFTADLALRADFGPAGGFLEMM
                     NVFDRAVPSSVMELSTGEAAPLPGRAFHLGLVWYLFD"
     gene            5648..6115
                     /locus_tag="VFR25_01925"
     CDS             5648..6115
                     /locus_tag="VFR25_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9939837.1"
                     /translation="MWGRELRAGLAFVLVSLLVGTLVREWQRDHRVRFEDLVADLEEA
                     HGPGSAPAPRADSSQTTATSTAKPTRTRRTPEPALRPGGLEMDRATASDWERLPGIGP
                     SLAARIVADRAERGPFRTPEGLLRVRGIGPRTLERIRPYLTPAPVDSGSPIAN"
     gene            6335..8035
                     /gene="pilB"
                     /locus_tag="VFR25_01930"
     CDS             6335..8035
                     /gene="pilB"
                     /locus_tag="VFR25_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET9939838.1"
                     /translation="MQQENIGLKLLEARLITPDALQKAQLQIKTGGGNLTGALVKIGA
                     IAEDDLAKFLSEFYAVPSVDLSNFNPDPSLTRILPSDVANKFQAVPISRSGRRLTVAM
                     ANPANIFAIDDIKFITGCEVQPCVATESAIKKAIDKAYDSAATFESVMRGLDDEIEVL
                     EDTTDDVPDVSLLGEAEQAPVVKLVNSLITDAVRKGSSDIHIEPYEKSLRVRFRIDGT
                     LYEMMSPPFRMKAAVTSRLKIMADLDIAERRVPQDGRIKLRLLGRNIDLRVSTLPTIF
                     GEKVVMRILDKGNLNIDLNKLGFQEQAFNDFTKAIAMPYGMVLVTGPTGSGKTTSLYS
                     ALSKINVPDVNIMTAEDPVEYNLEGINQVLVNEGVGLTFAAALRAFLRQDPNIVMVGE
                     IRDLETASIAVKAALTGHLVLSTLHTNDAPSTINRMVDMGIEPFLVASSTNLIMAQRL
                     IRRICKQCKTDVQLHAEVLRELGLSDDEAKKSSFKEGKGCVDCNNTGYRGRLGVYEVM
                     PITPKVRDLILDRAPTSQIKKVAVDEGMLTLRMDGLAKLKNGVTTAEEILKETAADRF
                     "
     gene            8049..9134
                     /locus_tag="VFR25_01935"
     CDS             8049..9134
                     /locus_tag="VFR25_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET9939839.1"
                     /translation="MISLRQLLDEMIKMRASDLHISAGVPPQARIDGSITAMGHPPLT
                     PQMTQQLAYSVLTEEQRKRFETTKELDLSFGVPGLSRFRANVFLQRGVTSMAIRQIPY
                     EIFSCEQLGLPKTIRDLTQKQKGMILITGPTGSGKSTTLAAMIDLINTSRAGHIITIE
                     DPIEYVHHHKKCIVHQREINSDTTTFATALKYVLRQDPDVILIGEMRDLETIAAALTI
                     AETGHLVLATLHTNSAYESVNRIVDAFPSSQQPQVLAQLAFVLEAVVTQQLIPRSRGP
                     GRVMVAETLLCTAAVRAVIRERKTHQIYSLMQAGSKFGMQTMNQGLFQAFVNKHIALD
                     EALGRSSDVKELEGMLEKVGVHISAAY"
     gene            9150..10373
                     /locus_tag="VFR25_01940"
     CDS             9150..10373
                     /locus_tag="VFR25_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9939840.1"
                     /translation="MPVYLWKGRTTAGEIQTGEMTFDSQDEVLSALRKKRIIITSVRE
                     KPRDLKITLPKIGSGVSTRDLAIFTRQFATMINAGLPLVQCLDILSKQTEKEDFRAVI
                     AQTMRDVEAGTTLAEALAKKENNKVFDELFVNMVEAGEAGGILDDILQRLATFIEKAE
                     ALKRKIQGAMVYPAVVMTVACLATAFMLIFIIPTFARMFTAFGGDLPLPTKIVMGLSS
                     FLRSYWWVMLGGIVGSIVMIRRYYTTEQGHMQIDKLLLRVPVLGDVIRKGAVARFTRT
                     LGTLISSGVPILTGLEITARTAGNRVVQEAIMAARASIREGETISAPLKQSNVFPPMV
                     VQMISVGEETGALDDMLTRIADFYDSEVDTAVDALTSLIEPVMIVFMGAVVGGMVIAM
                     YLPMFKLINVVAGGH"
     gene            10435..12108
                     /locus_tag="VFR25_01945"
     CDS             10435..12108
                     /locus_tag="VFR25_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9939841.1"
                     /translation="MNAVIFGRVALITLVATMAVAFSDTGLSLTRHYPFGFLILLSGG
                     LGFAYTRAVRSGADVNALLTTQFVVDVAMISYLLLFTGGTSSQFAPLFLLAPLMGGIF
                     LHVRGGLLFAGAAALAYTAFYVSERAGMVPPMAYGLTERLSDVALKLRLILYVPLFFI
                     VGAIGGAIGRSLREGDQALNEARAQLNRALFDTETILENLSSGLATIDAGGLVRHWNR
                     AASEILGIPAGAVRGRHYVEVFREGFEAFASRLRETLEQGVEGTRYEVTVRTADGRTV
                     SLGMSTSLLLGSAGERRGMIALFQDLSEVKALEEKIRRGETLVAIGQLSAGIAHEIRN
                     CLNPIAGSVEVLQRELQVEGENARLLDLIVRESERLDRFIKELLDYARERPLRTEAVD
                     AGTLAGESVELARRHPSATTAKRVAFEPSGTKIWIQADMDQMKQVLTNLLINALEAID
                     GEGTVSVAVRQDRRAAHGWGRPQSAPRAVLEIVDTGTGIQPEDLEHVFDPFFSTKQGG
                     TGLGLAIASRIVERHGGHLEVESRVGVGTTMRLWLPQAERPARTVAHAA"
     gene            12131..13519
                     /locus_tag="VFR25_01950"
     CDS             12131..13519
                     /locus_tag="VFR25_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9939842.1"
                     /translation="MAGEKILIVDDEESMIQFLSILLTKEGYEVQVARSGREALQRAE
                     EDGFDVVVTDIKMPGDIDGLGVLSGVKKIDPSVPVVIRTAFASQKSAIEALNRGAYQY
                     LEKTAKNDEIRLVIKNALAMRRVQSENVLLKRQLKKTDDSKEIIGHSEQIQRVLKLVE
                     RVADSDSTILIVGESGTGKELIAKRVHYLSRRAEGPFVSINCGALPKDLLESNLFGHV
                     KGSFTGAVRDQEGLLAVAGGGSFFLDEVGETLPATQVKLLRALQEREIIPVGGTKPRK
                     IDVRLIAATNADLEKAVAENRFRPDLFYRLNVIQIRLPALRERKEDIEEMVEHFLRKL
                     TAGKEPKTLSGAAKDSLLAYDWPGNVRELENVIERAVILTDDGVIEPKDLPERVLQGG
                     KGRGSLVIDNPTLTLDELEKEYIMKVLHHTAGQKKRASEILGINPSTLYRKLLSYGLE
                     HEREPIELEGLS"
     gene            13777..14187
                     /locus_tag="VFR25_01955"
     CDS             13777..14187
                     /locus_tag="VFR25_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9939843.1"
                     /translation="MIRNEKGFTLIELMIVVVIIGILAAIAIPNFVAMQDRAREASVK
                     ANMHSFQLAVEDFAVKNTGVYPVAANAAAVHANFPSGTYPTNPFTGVTDEAPQSWAAA
                     ASAQGRWATNSTTTSYSISGYGKAAALTLVLTNG"
BASE COUNT         2376 a         4821 c         4810 g         2188 t
ORIGIN      
        1 ctcgagcaac ggtcggcgcg cgcccggcga gaggcggcgg agctccaccg gacgtgcgtg
       61 gaagcagtcg agcggcgcat ccaggcctcg atggcgtctt cgtgacgccg tcctggcctt
      121 agtgtagcta gttccgagct aaggagacgc gtggaccgta gcctcgccat caagaagcgc
      181 gctcaggcgc tcgtgcagaa ggggcagatc gatgccgccc tcgccgagtt cgagaagctc
      241 ttcgagaccg gcgaccggga tccgtacgac ttcatcaccg tcgcggacct cctcgccaag
      301 cgcggcgcga tgcaggaggc ggttcgccgc taccgtcagg ccatcgagga atacggcaag
      361 gcggagctct acaagaacgc gatcgccgtc tgcaagaaga tcctccggat ttcgaaagag
      421 gatctccaga tccatcgctc cctgggcgag ctgtacggca aggaaggact ctacggcgac
      481 gctcagatcc actatctcga gttcgccgag ggctcgatcc gccgccacga gttcgaagcc
      541 gcgctgagcg cggtcgagca ggtcgttctc ctgtcgcccg acaacgacga cctctccgag
      601 aagctcgtct cgatcgcgat gaaggcggac ctcccggagc gcggcgggaa ggagctcctc
      661 cgacgcgcgg aacggatgga gcagcgcggc ctcatgtctc agggggcgga gcttcgcgag
      721 cgcgcctcaa ccctcgcccc agggctcgtg gcagaatccg cctccgcggc tgccgagtct
      781 tcgcccgaga tgccccgggc gcaagcgcat gccggccagg tggagctcga gcggagcgct
      841 ccggcgatgc cctacaatcc gtcgccgccc accggcttcg gcgacgcgcc ccccgcggag
      901 gccgccccgc cgctcgagct cgagagcgcc aacggatcgc ggtacgaagg cgccaggttc
      961 accccgccgg cgccagccgc cggcccgcca gccgctgcac cgccggccgc tgcaccgccg
     1021 gccgccgcac cgcccacccc agccgcggaa gcgccggtcg cggcgtcgtc gacggccgag
     1081 attcccgccg gccccgatcg gcatgaagcg atggccggag agcacctccg cgcggggcag
     1141 atcgagccgg ccgcggagga gttctggaag ggagcggagc tcgccttctt ccggtccgat
     1201 ctggcgcgcg ctcgcgcgct gctcgagtcg ctcctccaga tccggccgaa tcacgagccc
     1261 gcgctccgcc ggatggtcga catcacatca caggcggacg acacagcggc ggaggccaag
     1321 gcgcgtttcg agctgggcga gctctatctc ggacagcagg agtggggcct cgcgcgcgcg
     1381 gagtacatgc gcgccctcga gctagactcg tccaacgacc gtgcgcgcat gcgcgtggcc
     1441 cgcatcgacg ccatgaacgg agaggtcacc ggatcgccgg tcgatctgga caacctgccg
     1501 caccagaagc cggccgcctc cgtgcgcgtg cgcgacgatc agcccgcccc gaccgacagc
     1561 ctcgtcgacc tcgaagagat catcgacgag ttcaaggcgg gcgtgagcag cacgatctcg
     1621 ggcgaggacc acgagagcca ctacgacctc ggcatggcgt acatggagat ggggctctac
     1681 gacgaggcgg tgagcgaatt ccagatcgcg tcgaagggca gcgcgatcga gctcaagtgt
     1741 ctcgagatga tcgcgctctg ctatctggag aagaacgagc ccgcgacggc ggccaaggag
     1801 ctgaagcgcg cgctcgagct gcccggccat gggccggacg agacgatcag catccggtac
     1861 aatctaggca tggcgctcga acggctcggg aacctggaca aggctctgca gtacttcgag
     1921 gaggtgtatc tcctcaacgt cgacttcttc aaggtcgcga ccaaggtgcg cgagctgaag
     1981 cagcggatcg cggctggggg cgtgtagtgc ctgcttcgat cgtcgatctc cacatgcatt
     2041 cgaccgcgtc cgacggctcc ctcgcccccg ccgacgtcgt cgccctggcc cagcggaacg
     2101 gcgtcaagat catcgcgctg accgaccacg attccgtcga cgggcttccg gcggccctcg
     2161 agcgggcccg cgaggccggc atccggatca tccccggcat cgagctctcc gtgagcgagc
     2221 agaacatgga cgtccatctc ctcgcctacg gcttcgatac cacggatgcc cgcatgctcg
     2281 aggcgatcgc ccgctatcgc gagtcacggc acgagcgcgc gcggaagatc ctgacccgcc
     2341 tgaaggggct cggcatccgg atcccgatgg aggagatcga ggagatcgcg cggggcggcg
     2401 ccatcggccg cccgcacgtg gccgaggcgc tcatgacgaa cggccacgtc gagacgttcc
     2461 acgaggcgtt ccagcgctac ctgggtcacc acgcgcccgc ctacgtggcc aagcacaccg
     2521 tctcgctcga ggaagcggtg gaggtcgtgc gcgacgcggg gggcgtcacc gtgctcgccc
     2581 atcccggcac gctcaaccgc gacgcgtgga tcccgggtct cgcgcggcgc ggactcgacg
     2641 gcatcgaggt ctggcactcg aagcacggtc ccgcggagat caaccgctac cgggaggtcg
     2701 cgcgggcgca cggactcctc atgacgggcg gctccgactt ccacggagag cgcacccccg
     2761 acgtctccat cggcagcgtc gcggtccccg agcaggtgat cgccgatctc gacgaggcgc
     2821 tccgcgctcg tcgcgcgctc aaagcccacc cctagccgca tcaccggtcc gcgctcggac
     2881 cgccttgcac atccggacct ccatctggta gcgtcatcct tcctcctcgg cccatgcgaa
     2941 ccgtcgtccg aagcgccgtt ctcatcgctt tctcggtcgc gtgcggctac gcgaagttcc
     3001 tcctgtttcc ctatttgttc ttcgtggaga tcttcatcgt gtcggtgttc ctctccggct
     3061 cgctggtcgg ggtcgcgtgg ggcgcctggg tgggcgccgt ggcgggggcg gtcttcagcc
     3121 tggtgaatcc gtacggtgtc ccgcacccct gggtgatggc ggcgcaggtg ctctcggacg
     3181 cgctcgtcgg agccgccgga gggatcgccg gacggtggct tttacatgga tccggcgaga
     3241 cccggctcgg cgcgcgctcc cggaccgccc tcctcctcgg ggcgggaatc gtgctgacgg
     3301 cgatctacga cctcctcacg aacacggcgc agggcatcgc gtatggctcg ataccggcgg
     3361 ccctggcgct cgcggtcgtt ccgagcctcc agcacatcgc atcgaacgcc gcgatcttcg
     3421 cgatcgtcgg gaatctcgcg ttcccctggc tcgcacgtca tccggccgtg gtgcggcatg
     3481 cggcctgagc tgcgtccggt cgtcctgatg gccgggctcg tttgcgccct ggcagcggcc
     3541 ccgcgcgcgc gcgcccagtc cacggcgccg cgcgattcga cctcgcatgc catagcgccc
     3601 gtcgattcca cgcgacactc ggtctcggca gcgccgcacg actcgctcgc cttgcgcgcg
     3661 gcgcgccgcg attcgctccg tgctgtggcc gccgtgcgcg attccatccg gttcgcgcgc
     3721 gcgctccagg attccgcccg ctcggtcctg gcgcgccgcg actcgatctg ggccgaccgc
     3781 gcggggatcc agttcgagga gcagcgaaag cgaggcgaga tcacgctcga gcatgcgtgg
     3841 gtcggccgcc gcggcgcgat cctccgtgga ctgccgctcg ccggcattcc ggtcggcacg
     3901 ctgtccgtgc cggatgcggg atcgccgatc cgcaccgagc ctccaggctc ggaagggatc
     3961 gccgcgaccg accggccgct cgtctcggcc atgcccttcg gcgtcggcat cgtggatctc
     4021 cccgtcgcgc tcgattcgcc gcgcgcggaa ggggacgagc cgctcgacct ggtggaggtg
     4081 gaggccgtcc ccgcgccggg gtcgtatcgc cgcgcggggg agctcctcgc cgatccggcg
     4141 gcggagcagg tgttcgtcca cgcgatgccc ggaaccgacg ctcattatcg ccgggcgcga
     4201 agcgccctct attacggaaa cggcgacgag ggggagctcg ataccgcggc gcgcttcgca
     4261 tcggccacgt tcggatgggg gatcgcgggg tcgtacgcgc ggcatgaagc ggacggcctg
     4321 gcgcccctcc atcacgcgaa ggcgacgcgc tacgccgtcg cctcggggct tccgcgcgcg
     4381 ctccaccaca cgctctggat cgaggcgcgc gcgatggagt ggaacatcga ggacgaggcg
     4441 atcgggatcg atcccatcaa tggcacgccc ttcagtgcgg tcggccgcgc cgagatcagc
     4501 cgccgcgacg tcctcctcca tgggcaggcc ggcggctcga atgcgtccag cgtctggacc
     4561 gcccagctcg aagagctcaa gcgcacgcgc gtcgagggga cgggcgcgcg ccagcgctgg
     4621 atcttcccgg gcgtccaggc cacgtgggac ggacacgtcg atctcgatcg ccagtggtcc
     4681 gtgctggcgc acgccgaagg atcgtcgcga cggatccgct atgacgagaa cgcggcgccc
     4741 atcgtggacg gccgccgcga ggagggacgg ctcgggctcg ccctcgcgca cgcggacgcc
     4801 ggaggcggat cgcggctcga cgtcgcgtac gacgcccgcg agaccgcacc gtccttcgcc
     4861 gacgcccgcg cgtcactctg gaccgagcgc ggccggatgc gggggcggat cgatctcgag
     4921 cggtcgcacg agcggccgag ctgggtggac ctcgcgacgc ccgcgagcac gaccgaacgt
     4981 ctcgacccga ccttcaccca gcggctcatt ctcacccgct cgggagatcc ctcgctcgag
     5041 ccgaggcggc tcaccggcgg gctcgcgcgt ggcgcgctcg agctcggccc gaatctcgcg
     5101 gtcaccctcg aagggagcgc gcgctacgtg gaggacgatt tcggctggac gctgacgcgc
     5161 gtggcgactc cggagacgct cctcgtggac acgcgggcgg ccgttcgggg gagcggctgg
     5221 gtttcctatc tgggaagcgg ctttcgcgcg cgaacgggct ccgcgcgctg gcgcgcgttc
     5281 ggctgggttc gcggcggtcc ggacgacctc ggcccgaggt cgggcctcgt cccccgcatg
     5341 ggggcggacg tcgccgcgga cttcagggtc acgttcttcg agggcgacct gccgctcgag
     5401 ctgggagtgc gggggcatgc gctcggaccg agccacggca cgatcgaagc cgccggctcg
     5461 ttcacggccg acctggcgct ccgcgccgac ttcggccccg ccggcggatt cctcgagatg
     5521 atgaacgtct tcgaccgcgc ggtccccagc tcggtgatgg agctctcgac cggggaagcg
     5581 gccccgcttc ccggacgggc gttccacctc ggcctcgtct ggtacctctt cgactaggag
     5641 cgcggccgtg tgggggcggg agttgagggc ggggctcgcg ttcgttctgg tgtcgctcct
     5701 cgtcggcacg ctcgtccgcg agtggcagcg cgatcatcgc gtccgcttcg aggatctggt
     5761 cgcggatctg gaggaggccc acgggccggg atccgcccct gctcctcggg ccgactcgtc
     5821 ccagaccacc gctacgtcca cggccaaacc aacccgcacg cgccgcacgc ccgagccggc
     5881 gctccgtccg ggcggcctcg agatggatcg ggcgaccgct tcggactggg agcggcttcc
     5941 cggcatcggt ccgtccctcg ccgcccgcat cgtggccgac cgtgccgagc gagggccctt
     6001 ccggacgccg gaaggactcc ttcgcgtgcg cggcatcggc ccccgcacgc tcgagcgcat
     6061 ccgtccctac ctcaccccag caccggtcga ttccggctcg ccgatcgcaa actgagcagg
     6121 gcaatccgca cggttccgag ccgtcatcgc ggtcgtacgc ggcaaaacgc cgcacgcacg
     6181 ccgcgctaat cgccgttccc ctcatccgat cgcgcgtcgt gcccggcgcc atcgcggacg
     6241 tggcaccggt cttgcgaatc gaaggtccgc ccattcccgc gctcttggcg ggaacccgcc
     6301 acaacaacgc tagaacgaca ttgaggggac caccgtgcag caggagaaca tcggcctcaa
     6361 gctgctggaa gcacgcctca ttacgcccga tgcccttcag aaggcccagc tccagatcaa
     6421 gaccgggggc gggaatctga ccggggccct cgtcaagatc ggcgccattg cagaagacga
     6481 tctcgccaag ttcctctccg agttctacgc agttccctcg gtcgatctca gcaatttcaa
     6541 tccggatccc tcgttgacga ggattctccc gtccgacgtc gcgaacaagt tccaggcggt
     6601 tccgatctcc cgctcggggc gccgcctcac ggtggccatg gcgaatcccg ccaacatctt
     6661 cgcgatcgac gacatcaagt tcatcaccgg ttgcgaggtg cagccgtgcg tggcgacgga
     6721 atcggccatc aagaaggcga tcgacaaggc ctatgactcc gccgccacct tcgagagcgt
     6781 catgcgcggc ctcgacgacg agatcgaggt cctcgaggac acgaccgacg acgttccgga
     6841 cgtgtctctc ctcggcgagg cggagcaggc gccggtcgtc aagctggtca actcgctcat
     6901 caccgacgcg gtccggaagg ggtcgagcga catccacatc gagccctacg agaagtcgct
     6961 ccgcgtccgc ttccgcatcg acggcacgct ctacgagatg atgtcgcctc cgttccggat
     7021 gaaagccgcg gtcacgtccc gcctcaagat catggccgac ctcgacatcg cggagcgccg
     7081 cgtgcctcag gacggacgca tcaagctccg cctcctgggc cggaacatcg acctccgcgt
     7141 ctcgaccctc ccgaccatct tcggcgagaa ggtcgtgatg cgaatcctgg acaagggcaa
     7201 cctcaacatc gacttgaaca agctcggctt ccaggaacag gcgttcaacg acttcacgaa
     7261 ggcgatcgcg atgccgtacg ggatggtgct cgtgacggga ccgaccggaa gcggcaagac
     7321 gacctcgctc tactccgcgc tctccaagat caacgtgccg gacgtcaaca tcatgaccgc
     7381 ggaggacccg gtcgagtaca acctcgaggg gatcaaccag gtcctcgtga acgagggggt
     7441 cgggctcacg ttcgcggcgg ctctccgagc cttcctgcgc caggacccga acatcgtgat
     7501 ggtgggcgag atccgcgacc tggagaccgc atccatcgcg gtcaaggcgg cgctcaccgg
     7561 ccacctggtc ctctccacgc tccacaccaa cgacgccccg agcacgatca accgaatggt
     7621 cgacatgggg atcgagccct tcctggtcgc ctcgtccacg aacctgatca tggcccagcg
     7681 cctcatccgc cggatctgca agcagtgcaa gacggacgtg cagctgcacg ccgaagtgct
     7741 gcgcgagctg gggctcagcg acgacgaggc caagaagtcg agcttcaagg aaggcaaggg
     7801 ctgtgtggat tgcaacaaca cgggttaccg cggccggctc ggcgtctacg aagtgatgcc
     7861 gatcaccccc aaggtccgcg atctgatcct cgaccgcgcc cccaccagcc agatcaagaa
     7921 agtggccgtg gacgagggga tgctgacgct gcggatggac ggcttggcga aactcaagaa
     7981 cggagttacg acggccgaag aaattctgaa ggaaacggcc gccgaccggt tctaggcacg
     8041 ggggaaccat gatctcgctt cgccaactgc tggacgagat gatcaagatg cgcgcgtccg
     8101 atcttcacat ctcggccggc gtgcctccgc aggctcgtat cgacggctcg attacggcca
     8161 tgggccatcc gccgctcacg ccgcagatga cccagcagct cgcgtacagc gtgctgaccg
     8221 aggagcagcg gaagcggttc gagacgacca aggagctgga cctctccttc ggcgtcccgg
     8281 ggctctcgcg cttccgcgcc aacgtgttcc tgcagcgcgg cgtcacgtcc atggcgatcc
     8341 ggcagatccc gtacgagatc ttcagctgcg agcagctcgg gcttcccaag acgatccggg
     8401 acctgaccca gaagcagaag gggatgatcc tcatcacggg cccgaccggc agcggcaagt
     8461 cgaccacgct cgcggccatg atcgacctca tcaacacctc gcgcgccggc cacatcatca
     8521 cgatcgagga tccgatcgag tacgtgcacc accacaagaa gtgcatcgtg caccagcgcg
     8581 agatcaactc cgacacgacc acgttcgcga cggcgctcaa gtacgtgctg cgccaggacc
     8641 cggacgtgat cctgatcggt gagatgcggg atctcgagac gatcgcggcc gcgctcacca
     8701 tcgccgagac gggacacctg gtcctcgcga cgctccacac gaactccgcc tacgagtcgg
     8761 tcaaccgcat cgtggatgcg ttcccgtcga gccagcagcc ccaggtcctc gcgcagctcg
     8821 cgttcgtgct cgaggccgtg gtcacgcagc agctcatccc tcggtcccgt ggtccgggcc
     8881 gcgtgatggt ggcggagacg ctcctctgca cggcggccgt gcgggccgtc atccgggagc
     8941 ggaagacgca ccagatctac agcttgatgc aggccggctc caagttcggc atgcagacca
     9001 tgaaccaggg cctgttccag gcgttcgtga acaagcacat cgccctggat gaagcgctgg
     9061 gccggagctc cgacgtgaag gagctcgagg gaatgctcga gaaggtcggc gtccacatca
     9121 gcgccgccta ctaagcaggg ggtcgcatcg tgcctgtgta tctctggaaa ggccgaacca
     9181 ccgccgggga gatccagacc ggggagatga cgttcgactc ccaggacgag gtcctgtcgg
     9241 cgctccggaa gaagcggatc atcatcactt cggtccggga gaagcctcgc gacctcaaga
     9301 tcacgctccc caagatcggg agcggcgtga gcacgcgcga cctggcgatt ttcacgcgcc
     9361 agttcgcgac gatgatcaac gccggtctcc cgctcgtgca gtgtctggac atcctttcca
     9421 agcagacgga gaaggaggac ttccgcgccg tcatcgcgca gacgatgcgc gacgtggagg
     9481 cgggcacgac actggccgag gccctcgcga agaaggagaa caacaaggtc ttcgacgagc
     9541 tcttcgtgaa catggtcgag gccggcgagg cgggcggtat cctggacgac atcctccagc
     9601 ggctcgcgac gttcatcgag aaggccgagg ccctgaagcg aaagatccag ggggccatgg
     9661 tctacccggc cgtcgtcatg acggtcgcgt gccttgcgac cgccttcatg ctcatcttca
     9721 tcattccgac cttcgcgcgc atgttcaccg ccttcggcgg cgacctgccg cttccgacca
     9781 agatcgtcat ggggctctcg tcgttcctgc gctcgtactg gtgggtgatg ctcggcggca
     9841 tcgtggggtc catcgtcatg atccgccgct actacacgac cgagcagggg cacatgcaga
     9901 tcgacaagct cctcctgcgc gtgccggttc tcggggacgt catccggaag ggcgccgtcg
     9961 cgcgcttcac gcggacgctc ggaaccctca tctcctcggg cgttccgatc ttgacgggcc
    10021 tcgagatcac cgcccgcacg gcggggaatc gcgtggtcca ggaggcgatc atggccgcgc
    10081 gggcgtccat ccgcgagggc gagacgatct ccgcgccgct caagcagtcc aacgtgttcc
    10141 cgccgatggt cgtccagatg atctccgtcg gcgaggagac gggcgcgctc gacgacatgc
    10201 tcacgcgcat cgccgacttc tacgattccg aagtggacac agcggtcgac gcactgacct
    10261 cgctcatcga gcccgtcatg atcgtcttca tgggcgcggt cgtcggcgga atggtcatcg
    10321 ccatgtactt gccgatgttc aagctgatca atgtggtcgc cggcgggcac taagccgcgc
    10381 cgggtgccgc ccgcgccggc cctgcctgag cgcgagcggc ggcagcgaga ggcggtgaac
    10441 gcggtcattt tcggccgcgt ggcgctgatc acgctggtcg cgaccatggc ggtcgcgttc
    10501 tcggacacgg ggctcagcct gacgcggcac tacccgttcg ggttcttgat tctcctgagc
    10561 ggagggctcg gcttcgcgta cacgcgcgcg gtccggagcg gcgcggatgt gaacgcgctc
    10621 ctgacgacgc agttcgtggt cgacgtggcg atgatctcgt acctgcttct cttcaccggg
    10681 ggcacgtcga gccagttcgc gccgctcttc ctgctggcgc ctctgatggg agggatcttc
    10741 ctgcacgtgc ggggagggct cctcttcgcg ggcgcggcgg cgctcgcgta cacggcgttc
    10801 tacgtctcgg agcgggcggg gatggttccg ccgatggcgt acgggctgac ggagcgcctg
    10861 agtgatgtgg cgctcaagct ccggctgatc ctgtacgtgc cgctcttctt catcgtgggg
    10921 gcgatcggag gagcgatcgg ccggtcgctt cgcgaggggg atcaggcgct gaacgaagcc
    10981 cgcgcccagc tcaaccgggc gctgttcgac acggagacga tcttggagaa cctttcgagc
    11041 ggactggcca ccatcgacgc ggggggactg gtgcgtcact ggaaccgggc ggccagcgag
    11101 atcctcggga tcccggccgg cgcggtgcgg gggcgccact acgtcgaggt gttccgggag
    11161 ggattcgaag cgttcgcgtc ccggctccgg gagacgctcg agcagggagt ggaggggacg
    11221 cgctacgagg tgacggtacg cacggcggac gggcgcacgg tctcgctcgg gatgtcgacg
    11281 agcctgctcc tcggaagcgc gggcgagcgg cggggaatga tcgctctctt ccaggacctg
    11341 tccgaggtga aggcgttgga ggagaagatc cggcgcggcg agaccctggt ggcgatcggg
    11401 cagctctccg ccggcatcgc gcacgagatc cggaactgcc tcaatccgat cgcggggtcg
    11461 gtcgaggtcc tccagcgcga gctccaggtg gagggtgaga acgcgcggct cctcgatctc
    11521 atcgtgcgcg agtcggagcg gctcgaccgg ttcatcaagg agctcctcga ctacgcgcgc
    11581 gagcgcccgc ttcgcacgga ggcggtggac gccgggaccc tcgcgggcga gtcggtggag
    11641 ctggcccggc gtcatccgag cgcgacgacc gcgaagcggg tcgcattcga gccgtccggg
    11701 acgaagatct ggatccaggc ggacatggac cagatgaagc aggtcctgac gaatctcctg
    11761 atcaacgccc tcgaggcgat cgacggggag ggaacggtgt cggtggccgt gcgccaggac
    11821 cggagggcgg ctcatggctg gggacggccg cagagcgcgc cgcgcgcggt gctcgagatc
    11881 gtcgacacgg gcacgggcat ccagcccgag gacctcgagc acgtattcga tccgttcttt
    11941 tccacgaagc agggtggcac cggtctcggt ctggcgatcg ccagccggat cgtcgaacgc
    12001 catggcggac acctggaagt cgagagtcgg gtcggagtgg gcaccacgat gcgcttgtgg
    12061 ctgccccagg ccgagcgccc ggcacgcacc gtcgcgcacg cggcgtaacc ccatcggtga
    12121 ggatggtcca gtggccggcg agaaaatcct gatcgtcgat gacgaagaaa gcatgatcca
    12181 gttcctctcg atcctcctga cgaaggaggg ttacgaggtg caggtcgcgc gctccggccg
    12241 cgaggccctc cagcgggcgg aggaggacgg attcgacgtc gtggtcaccg acatcaagat
    12301 gccgggggac atcgacggcc tgggagtcct ctcgggcgtg aagaagatcg atccgagcgt
    12361 gccggtcgtc atccggaccg ccttcgcgtc gcagaagtcc gcgatcgagg cgctcaaccg
    12421 tggcgcctac cagtatctgg agaagacggc gaagaacgac gagatccgcc tcgtcatcaa
    12481 gaacgcgctg gccatgcgcc gggtgcagtc ggagaacgtg ctgctgaagc gccagctcaa
    12541 gaagaccgac gactcgaagg agatcatcgg gcactcggag cagatccagc gggtcctgaa
    12601 gctggtcgag cgcgtggccg attccgattc cacgatcctg atcgtggggg agagcggcac
    12661 gggcaaggag ctgatcgcga agcgcgtcca ctatctcagc cggcgcgccg agggtccatt
    12721 cgtctccatc aactgcggcg ccctgcccaa ggaccttctc gagtcgaatc tgttcggcca
    12781 cgtgaaggga tcgttcaccg gcgcggtgcg ggaccaggag ggtctcctcg ccgtggcggg
    12841 aggcggctcg ttcttcctcg acgaagtggg agagacgctg cccgcgaccc aggtgaagct
    12901 cctccgcgcg ctccaggagc gcgagatcat cccggtcgga ggcaccaagc cgcggaagat
    12961 cgacgtgcgt ctgatcgcgg cgacgaacgc ggatctcgag aaggcggtcg ccgagaaccg
    13021 cttccgtccc gacctcttct atcgcctcaa cgtgatccag atccgccttc cggcgctgcg
    13081 cgagcggaag gaggacatcg aggagatggt ggagcacttc ctgaggaagc tcaccgcggg
    13141 caaggaaccg aagacgctga gtggagccgc caaggactcg ctcctcgcct acgactggcc
    13201 gggaaacgtc cgggagctcg agaacgtgat cgagcgtgcg gtaatcctca cggacgatgg
    13261 cgtgatcgag ccaaaggatc tgccggagcg ggtgctccag ggcgggaagg ggcgcgggag
    13321 cctcgtcatc gacaacccga cgctcacgct cgacgagctg gagaaggaat acatcatgaa
    13381 ggtgctccac cacaccgccg gacagaagaa gcgcgcgtcg gagatcctcg ggatcaaccc
    13441 gtcgaccctc tatcgcaagc tcttgtccta cggcctggag cacgagcggg agccgatcga
    13501 gctcgaaggg ctgagctagg cccggcgcgc tcgcggcgcg cccgcgcgca tcgtgcaggc
    13561 tgcggtaggg ccggtctgcg tcgtgcgaat tgcgcgatgc agcgggcgcg aagcgcgcgc
    13621 ggcgataagg tccggcgtta cgtaatcacg gcagttgcgg ttggtatcag ggggctaccg
    13681 tgcgcgggag gtcatggcac ggacgttgca gtctaccccg gcatcgaggg acggttccgc
    13741 caaggacggc agtcacccca aacgaggaga cacgcaatga tccggaacga aaagggcttc
    13801 accctcatcg agctcatgat cgtggtggtc atcatcggta ttctcgccgc gatcgccatt
    13861 ccgaacttcg tggccatgca ggaccgggcc cgcgaagcga gcgtgaaggc caacatgcac
    13921 agcttccagc tggcggtgga agactttgcc gtgaagaaca cgggcgtgta tccggtggct
    13981 gccaacgcgg cggcggtgca tgcgaacttc ccgtcgggca cgtatccgac gaatccgttc
    14041 acgggcgtga cggacgaggc tccgcagtct tgggcggccg cggcgtccgc gcagggtcgc
    14101 tgggcgacga actccacgac gaccagctac tcgatctcgg gctatggcaa ggcggctgcg
    14161 ctgaccctgg ttctgaccaa cggttaagca gcggc
//
LOCUS       DASTKC010000045         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1217159, whole genome shotgun sequence.
ACCESSION   DASTKC010000045 DASTKC010000000
VERSION     DASTKC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217159"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VFR25_01960"
     CDS             complement(<1..1591)
                     /locus_tag="VFR25_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9939844.1"
                     /translation="MSAREPHGRLFRKYLVVLLVLVGGVLMASSLVELYFSYQETQRA
                     IVRVERAKATATAARIEQFMKDIERHVRETTQAASDDTEATRVGSRRSTFREGLSTAL
                     AEQRELDFLRLLRNVPAVTEISHLDVSGREQLRVSRVKFDATSSQDDFSRAPVFLEAR
                     TKTYVSPVYLRNGSDPYVTLAVPVGTNAVEVTVAQINLKSVLRLIAQLEVGTGGYAYV
                     LDSRGRLFAHPDARLVQENRDLSEMPQVKAARTRDGVVQDDDSKVTVADGLQGTRVLA
                     AHAAIAPLGWRVFVERPVADAYAPLRAPIIRSAVIFVLGLALSILASVLLARRMVAPI
                     QTLREGAARIGAGALDHRIEVKTGDELEALGQEFNRTAEQLQQSYAGLEQKVEARTRE
                     LAEALDQQRTLGEVSQIVSSTLDLQTVLTTIVTHAVQLSKTEAGTIYEFDEAEQVFVP
                     RANFGISEELIAALRDSRIHVGDTVIGKSAATRVAVQIPDLANEPNYRLFDVLEQAGF
                     RALLAVPLLREDRVIGALVVRR"
     gene            complement(1588..>2217)
                     /locus_tag="VFR25_01965"
     CDS             complement(1588..>2217)
                     /locus_tag="VFR25_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9939845.1"
                     /translation="FTLVSDPVAAGIVANLARPEGSVTGVTSLQAELVGKRLEILKIL
                     VPNVRRVWIVHHSGDTSAAPMISQALSAGPLLKIDMVPRGVTTPEDLALVLRSVRPGD
                     ALLAPETSNLDIPTAILQTSLSARIPAAFSAALWVGHGGLVSYGPDYYAHGFQAAYLV
                     ARILRGARPADVPVEETDKIDLAVNLKTADLLGITVPRKILLRADAFRR"
BASE COUNT          373 a          734 c          740 g          370 t
ORIGIN      
        1 tgcgacgcac gaccagcgcg ccgataaccc ggtcctcgcg caggagcggg accgccagga
       61 gcgcgcggaa acccgcctgc tcgagaacgt cgaacagacg atagttcggc tcgttcgcca
      121 gatcgggaat ctggaccgcg acccgagttg ccgccgattt cccgatcacc gtgtcaccca
      181 cgtggatccg cgagtcgcgg agcgccgcga tcagttcctc gctgatcccg aagttcgccc
      241 gcggcacgaa gacctgttcg gcctcgtcga actcgtagat tgtgccggcc tctgtcttgg
      301 agagctgaac ggcgtgagtg acgatggtgg tgagcacggt ctgcaggtcg agggtggagc
      361 tgacgatctg gctgacctcc cccaacgtcc tctgctgatc gagcgcctcc gccagttccc
      421 gcgtgcgggc ctccaccttt tgctccagcc cggcgtagga ttgctggagc tgctccgccg
      481 tgcgattgaa ctcctggccc aacgcctcga gctcgtcccc ggtcttcacc tcgatgcgat
      541 gatcgagggc gccagctccg atgcgagcgg ctccttcccg cagcgtttga atcggcgcca
      601 ccattcggcg agcgaggagg acgctggcca ggatcgacag cgcgagaccg aggacgaaga
      661 tgacggcgct ccggatgatg ggcgcgcgca gaggcgcgta ggcatccgcg accggccgct
      721 ccacaaagac gcgccatccg agaggggcaa tcgctgcgtg cgcggcgagc actcgcgttc
      781 cctgcaagcc gtccgcaacg gtgaccttgc tgtcgtcatc ctggacgacc ccgtctcgag
      841 tccgcgcggc tttcacctgg ggcatctcgg agagatcgcg gttctcctgg acgagccgtg
      901 cgtccggatg ggcgaagagg cggcctcgcg agtccagcac gtaggcgtag ccgcccgtgc
      961 cgacctcgag ctgcgcgatc aacctcagga ccgacttcag gttgatctgg gccaccgtga
     1021 cttcgaccgc gttcgtcccc accgggacgg cgagcgtgac gtaggggtcc gatccgttcc
     1081 ggaggtacac gggactgaca tacgtcttgg tccgtgcctc caggaagacc ggcgcccgcg
     1141 agaagtcatc ctgactgctg gtcgcgtcga acttgacgcg ggacactcgg agctgttccc
     1201 tacccgacac gtcgaggtgg ctgatctcgg tgacggcggg cacattgcgc agcaaccgca
     1261 gaaagtccag ctcgcgctgc tcggccagag cggtgctgag gccttcgcgg aaggtcgacc
     1321 ttctactgcc gacccgggtc gcttcggtat cgtcggaggc ggcctgggtt gtctcgcgca
     1381 cgtgacgttc gatgtccttc atgaattgct cgatgcgcgc ggcggtcgcc gtggctttcg
     1441 cgcgctcgac ccggacgatc gcgcgctgcg tttcctggta ggagaaatac agctcgacga
     1501 ggctcgacgc catcaacacc ccgccgacca ggaccagcag gacgacgagg tacttcctga
     1561 acaggcggcc gtgcggctcg cgagcgctca ccggcggaac gcgtccgcgc ggagcaggat
     1621 cttgcgcggc accgtgatac cgagaagatc cgcggtcttg aggttgacgg ccagatcgat
     1681 cttgtcggtt tcctcgacgg gcacgtcagc cggccgcgca ccccgaagaa tcctggcgac
     1741 gagataggcg gcctgaaagc catgcgcgta gtagtcggga ccgtaggaca ccaggccgcc
     1801 gtgaccaacc cagagcgcgg cgctgaacgc cgccggaatg cgggccgaga gggatgtctg
     1861 caggatggcc gtcggaatgt ccaggttgga ggtctctggt gccagcaggg cgtcgccggg
     1921 ccgcacgctc cggagcacca gggccaggtc ttccggcgtc gtaacacctc gcgggaccat
     1981 gtcgatcttc agcagtggcc ccgcgctcag agcctgggag atcatcggcg ccgcgctcgt
     2041 atcgcccgaa tgatggacga tccagacacg ccgcacgttc gggaccagga tcttgagaat
     2101 ctccaaacgc ttgcctacca gctcggcttg gaggctggtg actcccgtga cgctcccttc
     2161 cggacgcgcc aggttcgcga cgatgccggc ggccacggga tcgctgacga gcgtgaa
//
LOCUS       DASTKC010000046        13173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_35009, whole genome shotgun sequence.
ACCESSION   DASTKC010000046 DASTKC010000000
VERSION     DASTKC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35009"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFR25_01970"
     CDS             <1..704
                     /locus_tag="VFR25_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9939846.1"
                     /translation="FTGATQMHRGYFERASKGTLFLDEITEMPIELQVKLLRVLETGT
                     LVRVGGDEPISVDVRVIAASNRSPEQAVKDGKFREDLLYRLNVFPILLPPLRDRNGDI
                     ELLAEHFLAQLNREESANKKFSAVARRRLETYSWPGNVRELMNIVRRAFILAEESIEL
                     EGLPVPATAGVGASAGGAAPDAVRVGMSLAELERHFILATLEQYGGDKRKAAEVLGIS
                     LKTMYNRLNNYAAAV"
     gene            complement(735..2915)
                     /locus_tag="VFR25_01975"
     CDS             complement(735..2915)
                     /locus_tag="VFR25_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET9939847.1"
                     /translation="MRTSVDDLIQIQDRYYILASSSRIDDRTRVLKHDDLFAVLDRFG
                     ELAPQGFGELGLYYDGTRFLSRLGISLGHHRPLLLSSTLSRDNTRLLVDLTNPDLPAE
                     DGVVIPRGTIHLCREVLLWNGVAYDQLRLVNHGRTPVSVDLSIHLDADFTDIFEVRGT
                     HRERSGRRAPVVCSPTGMEFAYDGLDDRRRVTRVVFSEAPRSQSGSESVFALRLTPHE
                     PWTLVTTYACEISDRAAGGALTFRDALEKRHDDLATSLARDSTIVTSNPAFNDWLERS
                     IADLHMMVTRTAQGPYPYAGVPWFSTPFGRDGIWTALMTLWISPEIARGVLRFLAAHQ
                     ATEHDPARDAQPGKILHEARGGEMAALGEVPFGRYYGSVDATPLFVMLAGAYLERTGD
                     IAFAREIWPHVERAIEWIEGDGDPDGDGFVEYARRSRDGLVQQGWKDSFDSVSHANGD
                     LAEPPIALCEVQGYVVAALEAAAIMAERIGMNGDPGRLRARAELVRQRFEECFWCEEL
                     STYAIALDGAKRPCRVRSSNAGHALYTGTANESRAALVMQGLLAEDCFSGWGVRTLSL
                     KEARYNPMSYHNGSVWPHDNAVIAAGFARYGFHEAAARVLSALFEASLHFDLRRTPEL
                     YCGFGRTRDAGPTLYPVACAPQSWAAAAPFLLLKACLGLRIDAPRGLLTLVRPALPDF
                     IEEVHLKNVRVGDSCVSLRLDRNGPKVDVSVVERRGEVGVAIHS"
     gene            complement(2896..3978)
                     /locus_tag="VFR25_01980"
     CDS             complement(2896..3978)
                     /locus_tag="VFR25_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9939848.1"
                     /translation="MRIAQVSPLYESVPPRRYGGTERVVSYLTEELVAQGHDVTLFAS
                     GDSRTSARLVPATPRALRLDSRCRDPLAHHVLMIEAVFAQQGCFDLIHFHCDYLHFPL
                     SRRSRTPTLTTLHGRLDLPDLAPLYREYVDMPLVSISNAQREPIPRANWRQTIHHGLP
                     AGISRLYPDRGRYLAFLGRVSPEKRVDRAIEIAHRSGLPIKIAAKVDAADREYFEDVV
                     RPLLSGPGIDFVGEIAEEDKDAFLGHALALLFPIDWPEPFGLVMIEAMARGTPVIAFP
                     GGSVHEVVENGLTGFVVSEIPEAVRAVERVFSLDRAAIRREFERRFTAHRMARDYAGL
                     YARQIEERARRAFEPALRAEHANQRR"
     gene            4295..4480
                     /locus_tag="VFR25_01985"
     CDS             4295..4480
                     /locus_tag="VFR25_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET9939849.1"
                     /translation="MPRYGQAAQKSVERAMHKKKHGTLKSGRSGATVKSRKQAIAIGL
                     SEAREKGAKVPRSSKRK"
     gene            4528..5019
                     /locus_tag="VFR25_01990"
     CDS             4528..5019
                     /locus_tag="VFR25_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939850.1"
                     /translation="MSKLVTGIFQSRESAELASEELIRSGFAPEDISLLVSETTHGRE
                     FAVKQTTKAPEGAATGAAVGGALGAVAAGLAAVATISVPPLSFLAAGPVVAALTGLGA
                     GGAAGGLIGALTGLGIPEHEAKFYHGELSKGGILLGVYAHHDRVKLAREILDNAGAEK
                     TRG"
     gene            5349..5555
                     /locus_tag="VFR25_01995"
     CDS             5349..5555
                     /locus_tag="VFR25_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET9939851.1"
                     /translation="MNWDQIEGTWRQVRGKAREQWGKLTDDDLDVVAGRRDALIGKIQ
                     ERYGIARDEAERQVHDWSDRMTIH"
     gene            5692..7005
                     /locus_tag="VFR25_02000"
     CDS             5692..7005
                     /locus_tag="VFR25_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939852.1"
                     /translation="MLPLAAFLPCAIAILAFARMAIAQEGSGTGRYGDSTWVAPSYCV
                     EPVRDDARVAAPDHERTAETVLRTPFRLVFFPVRMVARGSEKVVGFASDYVVQPPQRV
                     EWLGVRIGPAFGISGTSGPAAGVSIQGHGGLGARSNLTATWSTKDTRKIHFRAAVTDR
                     HMPLGVTANASYDYLPNRRFYGLGNEAVYNKTIYLRRENAGGVGVHFGADPYRRVYAL
                     VGISDVNVGPGYTGVPRAQDVFSPDDVPGLESDTKVIGYGAGADLASTDSRTDPTLGV
                     HLRGRVLRKESIDEHNLDYYDWSGEARAYMPVLSKRRVIALRYVYQGVDQVGSSEEIP
                     FYRMPTSANEVRFAGFKGNRFVDRQLMLAHAEYRWVAWRRIWADLFLQYGEVAPYAEA
                     FKASEAHRSIGGGLRTRLSASTSARLEIGHSHEGTAVYLDLKGDF"
     gene            7005..8687
                     /locus_tag="VFR25_02005"
     CDS             7005..8687
                     /locus_tag="VFR25_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939853.1"
                     /translation="MARSDIRRGALALVVGWVLGAALAHPAAAATPHGTGVPIDSSAC
                     IDRDDHPISMPKVRDPNLAENLYREGIMDQLGSAFDIPDKILWALQPVGVTTNQRAVN
                     INKYDEVPASSWFTPRNHVRALKPEEIKNGPEGPVHPQPPYTIKSTKKGGVTPGFQIK
                     DAADRKWIVKLDPKGYPQLGSGAAVVSSRLVWAAGYNFSQDEAFSFTRDELKIDDELL
                     KGKDGEKPFTEEYLDAMLREGATANGRYYAAASLFLKGKPAGPLSFSNRRDDDPNDWY
                     HHKNRRELRGLYVVYSWVNNWDVKDQQSLDMFREDEGSKEGHLVHNLLDASGSLGAAG
                     QGPKQLPTGYEERIDLGWTMKRILTLGFVVEPWRKVKQESGIPSVGNFESEGFKPKGW
                     KAYQAIPPWREMTRADAYWGAKLVASFTDAQIAAAIDAAGYEDPTARDYLFRVLVDRR
                     NKIARTWFKEVTPLDFFVVEGNTLRFRDLAVDVGAAKPREYEVVTGKDKDGVVHAVRL
                     TSPELPLDEGPLARVHNPLILEIRAQGVDAKPVKVTLAHRGADWAVTEVRHE"
     gene            8684..10231
                     /locus_tag="VFR25_02010"
     CDS             8684..10231
                     /locus_tag="VFR25_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9939854.1"
                     /translation="MIPADGWSPGGADRIRAGLGVPAVLLALLVLLPAGCAKTGRLQY
                     VEQPTAHVSPAPGSPVPQPPMRALFGTDSLIGFVPQTGDSTRRYLGRLNRGFTADTLN
                     LILCGDNRPAFRTMRLRPEYQHVKHMVSLNPLKIARGLLAIPIILVKGMIPDAALVRD
                     LVPAIRHMPTYGAEKDVNKAMLAKVDSLERRGEAVTAVINTGDLVKDGRRPAHWERFL
                     GLIRPLSSRAPYLAVAGNHERTDTEDGLANWWAGTGLPISGDRLYYCFDSADGWVRFI
                     ALDSNPMTDPANFYSREVEVKYSDEQIDWMVARLKEHKGPAFVFLHNPPFSAGYHRTE
                     WQNDAVLRERRDRMVRALYEAKIGIIASGHEHAYQRAIFTWPDAVLINLVTGGAGSPL
                     HDIPGPAQSAALYSEYHVAGSVVKPENVYTGRFYHFIHMRLWFGGGEFYTYAVEKGGK
                     VRLADRVTIDLERFGVPEVDQQKMPVPQGKSGPQSEDLKKPKAASNDSTSAASKRIES
                     SPPPATK"
     gene            10261..11643
                     /locus_tag="VFR25_02015"
     CDS             10261..11643
                     /locus_tag="VFR25_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET9939855.1"
                     /translation="MSTRADAVPNARSSEPPRGVLDRALNLFADVRAGEGVTAVLLML
                     NIFLLLAAYYLLKTIREPLILTVQGGAEVKSYSAAAIAGLLMILVPVYSALASRVSRV
                     PLINGVTAFFILCLVAFFFLNQAGVAIGIPFFIWVGIFNLMVIAQLWAFANDVYTVDQ
                     GKRLFAIVGFGASLGAIAGSFFTGKLVSQYGPYPFLIAAAVLLGICMLLTNVVNAREK
                     ASKDGEGEGETEGDRAERSGDSSVKGRSGFALVFADRYLLLIAVLMLLSNLVNTTGEY
                     ILGKTVVANYTAHAGAAAAGGLDEKKIIGEFYGNYFTWVNVISALLQALVVSRILKYL
                     GVRAGLMVLPFVALFGYLAMAFIPVVAFIRGAKIAENSLDYSVQNTTRNALYLPTSRE
                     AKYKAKQANDTFFVRLGDLASAGLVFAGTTWLAFGPRQFALTAVALVGIWLIVAFVLG
                     KRFQSLAHTN"
     gene            complement(11659..12336)
                     /locus_tag="VFR25_02020"
     CDS             complement(11659..12336)
                     /locus_tag="VFR25_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9939856.1"
                     /translation="MLTPVERVLILKGVDLLKDVGPRHLLGLANVAREVEIFDGDSIY
                     EEKDAADALYIVVEGRVRLSTDGRTMSEVGPGEAFGTWSLVDDSARGHRAMCADSGRV
                     LALNREDFYDLAAADLTLLTELVRVLAKRLRALTAERPQEEQRVEGEGIEKPEALVQE
                     DEAAQTPEAVPPTAGSALAAAALGKPLPTPTESEPAPVAPDPSVAPGGVPIPKPIVPP
                     AEEKPLP"
     gene            complement(12348..>13173)
                     /locus_tag="VFR25_02025"
     CDS             complement(12348..>13173)
                     /locus_tag="VFR25_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939857.1"
                     /translation="SYDARLAIAALRNAAIPALQPYLSGVRGIRSQDRAARTLAQIAT
                     PAALRALLGLVRSSDRALRQLGLENASKIRVTVGRPVMSRTRVHKLFLRELREYREWI
                     VPSHAMAQHLAPEVRLLGESFREYADMALERGFRALSCWYDPKPLSGAFERLKTRGTE
                     DDAPALEYLSHVLPRTIFRHVETQFETLTAPSEDYAGDVSRGLAGWIASAWRSGDAWL
                     RACAVRASRWATDLEPGLFDTSEADSSSIVRAELKAVRSQSEEAPSWILTPRPLSP"
BASE COUNT         2228 a         4364 c         4495 g         2086 t
ORIGIN      
        1 gcttcacggg agccacgcag atgcaccgcg gctacttcga gcgcgcgtcc aagggaacgc
       61 tcttcctgga cgagatcacc gagatgccga tcgagctcca ggtgaagctc ctccgcgtgc
      121 tcgagaccgg cacgctggtc cgcgtgggcg gagacgagcc gatctcggtc gacgtccgcg
      181 tgatcgccgc gagcaatcgc tcccccgagc aggccgtgaa ggatgggaag ttccgcgagg
      241 accttctcta ccgtctcaac gtgttcccga tcctcctgcc gccgttgcgc gatcgaaacg
      301 gcgacatcga gctcctggcg gagcacttcc tcgcgcagct caatcgcgag gagagcgcga
      361 acaagaagtt cagcgccgtc gcgcggcgcc ggctggagac gtatagctgg ccgggaaacg
      421 tgcgcgagct catgaacatc gtgcggcgcg cgttcattct cgcggaggaa tcgatcgagc
      481 tggaaggcct gccggtgccg gcgaccgccg gcgtgggcgc gtccgcggga ggcgccgctc
      541 cggacgcggt ccgcgtgggg atgtcccttg cggagctgga gcgccacttc atcctcgcga
      601 cgctcgaaca gtacggcggg gacaagcgga aagcagcgga ggtgctcggg atcagcttga
      661 agacgatgta caaccgcctc aacaactacg cggccgcggt ctgacgggtg gcggccgcgt
      721 gagcggcggc cgccctacga gtggatcgcc acgccgacct ctcctcgacg ctccaccacg
      781 ctcacgtcca ccttcggacc gttccggtcc agacgaaggc tcacgcacga gtctcccacg
      841 cgcacgttct tgagatgcac ctcctcgatg aagtcgggga gcgcgggccg gacgagcgtc
      901 aaaagcccgc gcggcgcgtc gatccggagc ccgaggcacg ccttgagaag caggaaggga
      961 gcggccgcgg cccacgattg cggcgcgcag gcgacggggt agagcgtagg acccgcgtcg
     1021 cgcgtgcggc cgaagccgca gtagagctcc ggcgttctcc gtagatcgaa gtgaaggctg
     1081 gcttcgaaga gcgccgagag cacgcgtgcg gcggcctcgt ggaagccgta gcgcgcgaat
     1141 cccgccgcga tcaccgcgtt gtcgtgcggc cagaccgagc cgttgtgata cgacattggg
     1201 ttgtagcgcg cctccttcag ggagagcgtg cggacgcccc acccgctgaa gcagtcctcg
     1261 gccaggagcc cctgcatcac gagcgcggcc cggctctcgt tcgcggtgcc ggtgtagagg
     1321 gcatgccccg cgttcgacga gcggacccgg cacgggcgct tcgcgccgtc gagggcgatc
     1381 gcgtaggtcg agagctcctc gcaccagaag cactcctcga atcgctgccg cacgagctcc
     1441 gctcgcgctc ggagccggcc gggatcgccg ttcatgccga tgcgctccgc catgatggcc
     1501 gcggcctcga gcgcggcgac cacgtagccc tgcacctcgc agagcgcgat gggcggctcc
     1561 gccagatcgc cgttagcgtg agacacggaa tcgaaggagt ccttccaccc ctgctgcacg
     1621 agaccgtcgc gggagcggcg cgcgtactcg acgaagccgt cgccgtccgg gtcgccgtct
     1681 ccctcgatcc actcgatcgc gcgctcgacg tggggccaga tctcgcgggc gaacgcgatg
     1741 tctcccgtcc gctcgagata ggcgcccgcg agcatcacga agaggggcgt ggcgtccacg
     1801 ctcccgtaat agcgcccgaa cgggacctcg ccgagcgcgg ccatctcgcc cccgcgcgcc
     1861 tcgtggagaa tcttgcccgg ctgcgcgtcg cgcgcgggat cgtgctcggt cgcctggtgg
     1921 gccgcgagaa agcgaagaac gccgcgggcg atctcagggc tgatccagag ggtcatgagc
     1981 gcggtccaga tcccgtcccg tccgaacggc gtgctgaacc acggcacgcc ggcatacgga
     2041 tacggtccct gggccgtccg ggtgaccatc atgtggagat cggcgatcga gcgctcgagc
     2101 cagtcgttga acgcggggtt cgaggtcacg atcgtcgaat cgcgcgcgag gctcgtggcc
     2161 aggtcgtcgt gccgcttctc gagcgcgtcg cggaaggtca gcgctccacc cgccgcgcgg
     2221 tcactgatct cgcacgcgta ggtcgtcacg agcgtccacg gctcgtgcgg cgtgaggcgc
     2281 agggcgaaga ccgactccga gcccgactgc gaccggggcg cctccgagaa gacgacgcgc
     2341 gtcacccggc gacggtcatc gagcccgtcg tatgcgaact ccattcccgt gggcgagcag
     2401 acgaccggcg cgcgccgacc gcttcgctca cggtgcgtgc cgcggacctc gaagatgtcg
     2461 gtgaaatccg cgtcgagatg gatcgaaagg tcgaccgaga cgggggttcg cccgtgattg
     2521 acgagccgga gctggtcgta ggcgactccg ttccacagga ggacctcgcg gcaaaggtgg
     2581 atcgttccac gcggaatgac caccccgtcc tccgccggga gatcgggatt cgtgagatcc
     2641 accaggagcc gcgtattgtc ccggctgagc gtggagctca ggaggagcgg acggtgatgc
     2701 cccagggaga tgccgagccg cgagaggaag cgcgtcccgt cgtagtagag cccgagctcg
     2761 ccgaagccct gcggggcgag ctccccgaag cggtcgagca cggcgaaaag gtcgtcgtgc
     2821 ttgagcacgc gggtgcgatc gtcgatccgc gacgagctgg ccaggatgta gtagcggtcc
     2881 tggatctgga tcaggtcatc gacgctggtt cgcatgctct gcgcgcaacg ctggttcgaa
     2941 tgctctgcgc gcacgctcct cgatttggcg cgcgtagagc ccggcgtagt cgcgcgccat
     3001 gcggtgggcc gtgaagcgcc gctcgaactc gcggcggatg gccgcccggt cgagggagaa
     3061 gacgcgctcc accgctcgga ccgcttccgg gatctcgctc accacgaacc cggtgaggcc
     3121 gttctccacc acctcgtgca ccgagccccc cggaaacgcg atgacgggcg tgcctcgcgc
     3181 catggcctcg atcatcacga gtccgaacgg ctcgggccag tcgatcggga agaggagcgc
     3241 gagcgcgtgg cccaggaacg cgtccttgtc ctcctccgcg atctctccga cgaaatcgat
     3301 gccggggccg gagaggaggg gacgcaccac atcctcgaag tactcgcgat cggccgcgtc
     3361 caccttggcc gcgatcttga tcgggagacc tgaacgatgc gcgatctcga tggcgcggtc
     3421 cacccgcttc tccggagaca cgcgtcccag gaaggcgaga taccggccgc ggtccgggta
     3481 gagcctcgag atgcccgccg ggaggccgtg gtggatcgtc tggcgccagt tggcgcgtgg
     3541 aatgggctcg cgctgcgcgt tcgagatcga gacgagcggc atgtcgacgt attcgcggta
     3601 gagcggtgcg agatccggca ggtcgagccg cccgtgcagc gtggtcaggg tcggcgtgcg
     3661 ggatcgccgc gagagcggga agtggaggta gtcgcagtgg aagtggatga gatcgaagca
     3721 cccctgctgg gcgaacaccg cctcgatcat cagcacgtgg tgcgcgagcg ggtcccggca
     3781 gcggctgtcc aggcgaagag cgcgcggcgt ggctggaacg aggcgcgccg acgtccgtga
     3841 atcgccgctc gcgaacaggg tcacgtcgtg cccttgtgcc acgagctcct cggtcaggta
     3901 actgacgacg cgctccgtgc ctccgtacct gcgtggaggc acggactcgt agaggggtga
     3961 tacctgcgcg atgcgcaggc gctctcggac cactcggtcg gggttcacga tccctcgaat
     4021 agcaggcatt gtgccgacgc tatgtcccgg tttcgtgagg taaccgcgtg taggtttggg
     4081 ggatttcggt ggaaggcatt gcacagacga ccttggatcg ttccacggat gtcccatagc
     4141 ggtcccgtaa cagaggggga acgctcgccg tggcatgaac tctgcgatgt cattcgttcg
     4201 tccggttcgc gcaacgggct gcgcgcgttc gatcggacct gggatggaag cagcacgtca
     4261 aggagaggac acaagagccc ggagggaagc actcatgcca cgatacggac aagccgcgca
     4321 gaagtcggtc gagcgcgcga tgcacaagaa gaaacacgga acgttgaaga gcggaaggag
     4381 cggagccacg gtcaagagcc gcaagcaggc catcgcgatc ggactcagcg aggcgcgcga
     4441 gaaaggcgcc aaggttccgc ggtcttccaa gcgcaaatag aaccggatcc gacccgttcc
     4501 gacgggcagg aataaaggag gttcgacgtg tctaagctag tcactggaat ctttcagtct
     4561 cgagaaagcg ccgagctcgc gagcgaagag ctcatccgct ccggcttcgc gcccgaagac
     4621 atcagccttc tcgtttccga gaccacgcac gggcgggagt tcgccgtgaa acagaccaca
     4681 aaggcgcccg aaggggcggc gaccggggcc gccgtcggcg gtgcgctcgg cgccgtggcc
     4741 gcgggtctcg cagcggtcgc cacgatctcg gtacccccgc tttcgttcct ggctgcgggg
     4801 cccgtggtcg cggccctgac tgggctcggg gccggcggag ccgccggggg cctgatcggc
     4861 gccctgaccg gactcggcat ccccgagcac gaagcgaagt tctatcacgg tgagctgagc
     4921 aagggcggca tcctgctcgg cgtctatgcc caccacgacc gggtgaagct ggcccgggag
     4981 atcctggaca acgcgggagc ggaaaagacc cggggctgac cgtcccagga agaccgaggg
     5041 gctccgccgt cgaggcggag ccccattttg taaatgccgt tccctaccga tgtaggaatt
     5101 acaccgccta ccccatcagc cccgtgacac tactgatatt caacaagtta cgcattcctt
     5161 caaaacggca tgatagctgc ttttgcccgg gtcacgagct tacgggccgg caaccgaccg
     5221 gccccaacga tgcgagcagg cgctgggggt tcgggctaac gactgggccg attatgacgg
     5281 cagtatgttc gccccgcatt ctggctctgc tccaatttaa agaccgccct ttgggttggg
     5341 gggacgcgat gaactgggat cagatcgaag gcacttggag gcaagtccgt ggcaaggctc
     5401 gggagcagtg gggcaagctc acggacgacg atctggacgt ggtcgccggg cggcgcgatg
     5461 cgctcattgg caagatccag gagcgctacg gaatcgcccg cgacgaagcg gagcgtcagg
     5521 ttcatgactg gtcagaccgc atgacgatcc actgatccgg tccccagtcg tccgtcgtaa
     5581 gggggccctc caacggcctt ccaatgggaa ctgggcggtt ccccacccaa cccattcccc
     5641 ctagataagg agggccctct gactttttcc ttcatcaacc tcggtttccg aatgctgccg
     5701 ctcgcggcat tccttccgtg cgccatcgcg atcctcgcgt tcgcgaggat ggccatcgcg
     5761 caggagggtt ccggcacggg gcgctatgga gacagcacct gggtcgcccc gtcctactgc
     5821 gtcgagcctg tgcgtgacga tgcgagggtc gcggctcccg atcacgagcg caccgctgag
     5881 actgtcctcc gcactccgtt ccgcctggtc ttcttcccgg tgcgcatggt cgcgcgcggc
     5941 agcgagaagg tcgttgggtt cgcgagcgac tacgtcgtcc agccgccgca gcgcgtggag
     6001 tggctcggcg tacgcatcgg tcccgcgttc gggatctccg gaacatcggg tcctgcggcg
     6061 ggcgtgagca ttcaggggca cggcggcctc ggcgcccggt cgaatctgac cgcgacctgg
     6121 tccaccaagg acacgcgcaa gatccacttc cgtgcggccg tcaccgatcg gcacatgccg
     6181 ctcggcgtca ccgcgaacgc gagctacgac tacctcccca accggcgctt ctacggcctg
     6241 ggtaatgagg cggtctacaa caagaccatc tacttgaggc gcgagaacgc cggcggcgtc
     6301 ggcgttcact tcggggccga tccgtatcgg cgcgtctacg ccctcgtcgg catatcggac
     6361 gtgaacgtgg gcccaggcta cactggcgtg ccgcgtgccc aggacgtgtt ctcgccggat
     6421 gacgtgccgg ggctggagtc ggataccaag gtgatcggct atggggccgg cgcggacctg
     6481 gcctcgaccg actcgcgaac cgatcccaca ctcggcgtgc acctccgggg ccgcgtgctg
     6541 cgcaaggaga gcatcgacga gcacaacctc gactattacg actggtccgg cgaagcgcgc
     6601 gcgtacatgc ccgtgctctc gaagcggcgg gtgatcgcgc tgcgctacgt ctaccaaggg
     6661 gtcgaccagg tcgggagctc ggaggagatc ccgttctacc gcatgccgac gtcggccaac
     6721 gaggttcgct tcgccggctt caagggcaac cggttcgtgg atcgccagct gatgctggcg
     6781 cacgcggagt accgctgggt cgcgtggcga cgcatctggg ccgatctctt cctccagtac
     6841 ggggaggtgg ccccctatgc cgaggcgttc aaggcgagcg aagcgcaccg ctccatcggg
     6901 ggcggtctcc gcacgcgttt gagcgcgtcg acctcggcgc gtctcgagat cgggcacagc
     6961 cacgagggca ccgcggtgta cctcgatctg aagggggact tctgatggcg cgctcggaca
     7021 tccggcgtgg cgcgctcgcg ctcgtcgtgg gctgggtcct cggggctgcg ctggctcatc
     7081 ccgcggccgc cgcgaccccg cacggcacgg gcgtcccgat cgattcctcc gcctgcatcg
     7141 atcgggacga ccatcccatc tcgatgccca aggtgcgcga tcccaatctc gcggagaacc
     7201 tctaccgcga ggggatcatg gatcagctcg ggagcgcgtt tgacattccg gacaagatcc
     7261 tctgggcgct ccagccggtc ggcgtgacca cgaaccagcg cgcggtcaac atcaacaagt
     7321 acgacgaggt gccggcgtcc agctggttca ccccgcgcaa ccacgtgcgc gcgctcaagc
     7381 cggaggagat caagaacggc ccggaaggtc ctgtccatcc ccagccgcct tatacgatca
     7441 agagcaccaa gaaggggggc gtgacgccgg ggttccagat caaggacgcg gcggaccgca
     7501 agtggatcgt gaagctcgat ccgaaggggt atccgcagct cggatccggc gcggcggtcg
     7561 tgtcgagccg gctcgtctgg gccgcgggct ataatttctc ccaggacgag gcgttctcgt
     7621 tcacgaggga cgagctcaag atcgacgacg agctcctgaa gggcaaggac ggtgagaagc
     7681 cgttcaccga ggaatatctg gacgccatgc ttcgcgaggg cgcgaccgcg aacggccggt
     7741 actacgctgc cgcgagcctc ttcctcaagg ggaagccggc cggcccgttg agcttcagca
     7801 accgtcgcga cgacgatccg aacgactggt accaccacaa gaatcgacgg gagctaaggg
     7861 gcctctacgt ggtctactcc tgggtcaaca actgggacgt gaaggaccag cagtccctcg
     7921 acatgttccg cgaggacgag gggtcgaagg aggggcatct cgtgcacaac ctgctcgacg
     7981 cgagcggatc gctcggcgcc gcgggtcagg gtcccaagca gcttccgacc ggctacgaag
     8041 agcggatcga cctcggctgg accatgaagc ggatcctgac cctcggcttc gtcgtcgagc
     8101 cctggaggaa ggtgaagcag gagtccggca tcccctccgt gggcaacttc gagtcggagg
     8161 gattcaagcc gaagggctgg aaggcctatc aggcgatccc tccctggcgc gagatgacgc
     8221 gcgccgacgc ctattggggg gcgaagctcg tggcgtcctt cacggatgcg caaattgccg
     8281 ctgccatcga tgccgccggc tacgaggacc cgaccgcgcg cgactacctg ttccgcgttc
     8341 tcgtggatcg ccgcaacaag atcgcgcgga catggttcaa ggaagtcacg ccgctcgact
     8401 tcttcgtcgt cgaggggaac acgcttcgct tccgggacct cgcggtcgac gtcggagccg
     8461 cgaagccgcg cgaatacgaa gtggtcacgg gcaaggacaa ggacggcgtg gtccatgccg
     8521 ttcgcctgac gtcaccggag ctcccgctcg acgaggggcc gctcgcccgc gtccacaatc
     8581 cactcatcct cgagatccgc gcccagggcg tggatgcgaa gccggtcaag gtcacgctcg
     8641 cgcatcgtgg cgccgactgg gcggtcacgg aggtccggca cgaatgatcc cggcggacgg
     8701 ttggtcgccg ggaggcgcgg atcgtattcg cgccggcctt ggcgtgcccg ccgtcctcct
     8761 ggcgcttctc gttctccttc ccgccggatg cgcaaagacc ggacgccttc agtacgtgga
     8821 gcagccgacg gcgcacgttt cgcccgcccc gggctcgccc gtgccgcagc ccccgatgcg
     8881 cgcgctgttc gggaccgaca gcttgatcgg cttcgtgccg cagaccggcg attcgacccg
     8941 gcgctacctg ggcaggctga accgtggatt cacggccgac acgctcaacc tcatcctttg
     9001 cggcgacaac cgccccgcgt tccgcacgat gcgccttcgc cccgagtacc agcacgtcaa
     9061 gcacatggtc tcgctcaacc cgctcaagat cgcgcgcggg ctcctggcga tcccgatcat
     9121 tctcgtgaag ggaatgatcc cggacgcggc cttggtgcgc gatctggtgc cggcaatccg
     9181 ccacatgccc acctacggcg cggagaagga cgtcaacaag gccatgctcg cgaaggtgga
     9241 cagcctggag cgccgcgggg aagccgtcac cgcggtgatc aacacggggg acttggtcaa
     9301 ggatgggcgg cgcccggccc attgggagcg ctttctcggg ctcatccggc cgctgtcgag
     9361 ccgcgcgccc tatctcgcgg tcgccggaaa tcacgagcgc acggacaccg aggacgggct
     9421 cgcgaactgg tgggcgggga ccggccttcc gatcagcggg gaccggctct actactgctt
     9481 cgactcggcg gacggctggg tccggttcat cgcgctcgac tcgaatccga tgaccgatcc
     9541 cgcgaacttc tattcgcggg aggtcgaggt gaagtactcc gacgagcaga tcgactggat
     9601 ggtggcgcga ctcaaggagc acaagggccc ggccttcgtg ttcctgcaca acccgccgtt
     9661 ctcggcggga taccatcgca ccgagtggca gaacgacgcc gtgctgaggg agcgacggga
     9721 ccgcatggtg cgcgccctct acgaggccaa gatcggaatc atcgcgagcg gacacgagca
     9781 cgcgtaccag cgcgcgatct tcacgtggcc cgacgcggtg ctcatcaatc tcgtcacggg
     9841 aggggcgggc tcgccgctcc acgacattcc cgggccggcg cagagcgccg ccctgtactc
     9901 cgagtaccat gtggcgggat cggtcgtgaa gccggagaac gtctacacgg gacgcttcta
     9961 ccatttcatc cacatgcgac tatggttcgg aggcggggag ttctacacgt acgcggtcga
    10021 gaaggggggc aaggtgcgcc tggccgatcg cgtgaccatc gacctcgagc gctttggcgt
    10081 accggaggtc gaccagcaga agatgccggt gccccagggg aagtcgggac cgcagtcgga
    10141 ggatttgaag aagcccaagg ccgcatccaa cgactccacg tccgccgcca gcaagcggat
    10201 cgagtcgagt ccgccgcccg ccacgaagtg acgcgggcct gaatcaccga ggggggatcc
    10261 atgagcactc gagccgacgc cgttcccaac gcccgctcca gtgagccgcc acgcggcgtg
    10321 ctcgaccgcg cgctgaacct cttcgcggac gtgcgcgcgg gagagggtgt cacagccgtt
    10381 ctcctcatgc tgaatatctt cctcctgctc gcggcgtact acctgctgaa gacgatccgg
    10441 gagccgctca tcctcacggt ccagggcggg gccgaggtga agagctactc cgccgcggcg
    10501 atcgcggggc tcttgatgat cctcgtgccg gtctacagcg cgctcgcgtc gcgcgtgtca
    10561 cgcgtgccgc tcatcaacgg ggtcacggcg ttcttcatcc tgtgcctcgt cgcgttcttc
    10621 ttcctgaatc aggctggcgt ggcgatcggg atccccttct tcatctgggt ggggatcttc
    10681 aacctgatgg tcatcgcgca gctctgggcc ttcgcgaacg acgtgtatac ggtcgaccag
    10741 gggaagcggc tcttcgcgat cgtaggattc ggggcgtcgc tcggcgcgat cgcgggctcc
    10801 ttcttcacgg ggaagctcgt gtcgcagtac ggtccctacc cgttcctcat cgccgccgcg
    10861 gtgctccttg gcatatgcat gctcctcacg aacgtggtga acgcgcgcga gaaggcgagc
    10921 aaggacggag agggcgaggg ggagaccgaa ggagaccggg ccgagaggtc cggtgactcg
    10981 agcgtcaagg gacgctccgg cttcgccctc gtcttcgcgg accgctatct cctcctcatc
    11041 gccgtgctca tgctcctcag caatctcgtg aacacgaccg gcgagtacat cctcggcaag
    11101 accgtggtcg cgaactacac ggcgcacgcg ggagcggctg cggcgggagg cttggacgag
    11161 aagaagatca tcggggagtt ctacggaaac tacttcacct gggtgaacgt catctcggcg
    11221 ctcctccagg ccctcgtcgt gtcgcgcatc ctcaagtacc tcggcgtgcg cgcggggctc
    11281 atggtgctcc cgttcgtggc cttgttcggc tacctcgcga tggcgttcat ccccgtcgtc
    11341 gcgttcatcc ggggcgcgaa gatcgccgag aactcgctcg actactcggt ccagaacacc
    11401 acgaggaacg cgctctacct cccgacgagc cgcgaggcca agtacaaggc caagcaggcg
    11461 aacgatacct tcttcgtgcg gctgggagac ctcgcgtcgg ctgggctcgt cttcgcggga
    11521 accacgtggc tcgcgttcgg tccgcggcag ttcgcgctca ccgcggtcgc gctcgtcggg
    11581 atctggctca tcgtcgcgtt cgtgctgggg aagcgcttcc agtcgctcgc gcacacgaac
    11641 taggcggccg aaaccgcctc agggaagagg cttctcctcg gccggaggca cgatcggctt
    11701 cgggatcgga acgccccccg gcgcgacgga cgggtcgggt gcgaccggtg cgggttccga
    11761 ctcggtcggg gtcggcaggg gcttgcccag cgctgccgcg gccagcgccg atccagccgt
    11821 gggaggcacc gcctcgggag tctgcgcggc ctcgtcttcc tggacgagcg cctcgggttt
    11881 ctcgatgcct tccccttcga cgcgctgctc ttcctgcggg cgctcggccg tgagcgcgcg
    11941 aagacgcttc gcgagcactc gaacgagctc ggtcaagagg gtcagatccg cggcggccag
    12001 gtcgtagaag tcctcccggt tgagcgccag gacgcgcccc gagtcggcgc acatggcgcg
    12061 gtgcccgcgc gccgagtcgt ccaccaggga ccaggttccg aacgcttcgc ccggccccac
    12121 ttcggacatg gtccggccgt cggtcgagag gcgcacgcgc ccctcgacga cgatgtagag
    12181 cgcgtcggca gcgtccttct cctcgtagat cgagtcccca tcgaagatct ccacctcgcg
    12241 cgcgacgttg gcgaggccga ggagatggcg cggccccacg tccttgagga gatccactcc
    12301 cttcaagatc agaacgcgct cgaccggtgt cagcatgccg tcctctccta aggggagagg
    12361 ggccgcggcg tcaggatcca cgacggcgcc tcctccgatt gagaccggac cgccttgagc
    12421 tcggcccgaa cgatggagct cgaatccgct tccgacgtgt cgaacaagcc cggctcgaga
    12481 tcggtggccc agcgggacgc gcgcaccgcg cacgcgcgga gccaggcgtc gcccgaccgc
    12541 cacgcgctcg cgatccaccc agcgagccct cgactcacat cgccggcata gtcctcggag
    12601 ggagcggtca gggtctcgaa ctgagtttcc acgtgacgga agatggtccg gggcagcacg
    12661 tggctcaagt actcgagcgc cggcgcatcg tcctccgtcc ctcgcgtctt gagccgctcg
    12721 aacgctccgg agaggggctt gggatcgtac cagcacgaga gcgcgcggaa tccccgctcg
    12781 agcgccatgt ccgcgtactc gcggaagctc tcccccagga gccgcacctc cggtgctaag
    12841 tgctgggcca tcgcgtggga gggcacgatc cactcgcggt attcgcggag ctcgcgcagg
    12901 aagagcttgt gcacgcgcgt gcgggacatc accggtcgac cgaccgtgac gcggatcttg
    12961 ctcgcgttct cgagcccgag ctggcggagc gctcggtcgc tgctccgcac gagtccgagc
    13021 agggcgcgga gcgcggccgg cgtggcgatc tgagcgagcg tgcgcgccgc ccggtcctgc
    13081 gaacgaatcc cacgcacgcc cgagagatag ggctggagcg cgggtatggc cgcgttccga
    13141 agagccgcga tcgcgagccg cgcatcgtag ctc
//
LOCUS       DASTKC010000047         6058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_35577, whole genome shotgun sequence.
ACCESSION   DASTKC010000047 DASTKC010000000
VERSION     DASTKC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35577"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFR25_02030"
     CDS             <1..405
                     /locus_tag="VFR25_02030"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HET9939858.1"
                     /translation="TQLEVLRYIATEEEGRDMDRLKTSEEKLTFWTNFWKARDPSPET
                     PQNEARDEFYKRVTYANQHFSAGTQGWRTDMGRIYIKYGQPDEVVRNPFRFEGPPEEI
                     WYYYRARYTFFFVDEKGFGQYQIDDSRSSKID"
     gene            892..2070
                     /locus_tag="VFR25_02035"
     CDS             892..2070
                     /locus_tag="VFR25_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939859.1"
                     /translation="MNIYPYPTSVVRQNNLVLAELGHNPDGDVNPVTKETEQSLTIIK
                     NFTNFGAIAFQMEQNDNNSSFPNNLTNQQIDVIWGKGFDKLDLAVRLDLTNSKIEISD
                     NANNSAESHGIDTFGGALDPYPFGAFNPDAILSGPFELNTFGVTPAVALHLSNDNRVE
                     AALTLRKYSLDRSITTGGVAGESWEDNGNMSYALFVRGIMNKGDRATWYPAAWYVNDD
                     LGYTVTNSGIGVEPRDIDETYKTWGVGLSHNMRVNDNNLLIFGASVGEAKHEYDRNDS
                     NDGALNAETKTFEEKTMLLPIFFAGLETEATSWLKIRMGATQSLENNETETTAFDGTS
                     LKIKTHESSFDFNLGAGIRWNNLDFDLTLNEAFPMSGGWILSGDEATPATRASATYHF
                     "
     gene            2427..5378
                     /locus_tag="VFR25_02040"
     CDS             2427..5378
                     /locus_tag="VFR25_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9939860.1"
                     /translation="MTRLRVRRGLGVATVLIVGCALFVFGGTARAGTAGGITGTIRDA
                     ATSEPIGLATVTIPELKRGATTDAQGHYFILNLLPGKYTVRVALLGYIPQVREGVEVF
                     PDFNAKVDFTMTSTVLKDVQEVQVKGERPLIQKDVTTTTKFLNGDEIKNQPLRGYQEA
                     VAQQAGVVSFNLNNLNQVSAGTEIQNSNTLIIRGGRPNEVAYYVDGFSQQDPLTGFST
                     TSIANDAIDEVVVQSGGFNAEYGRINSGIVNVVTREGGDRYAGSVEGQLGDYLDRRGD
                     HTILAASLGGPITPKFKNLTFFLSGEHQNIDDRKPSFITDELFLPQQPGVFEDGVLPA
                     NDSESWATAAKMAYKLSPTKTIRVGGTYNKDDWRQFLSSYRFNLAHSPRYKDENWSAF
                     GSWNHTVNNRSFYELRANMFSTERIRGDGIYFDDLRGYARPEGNPSFDDLALYWNGDD
                     PATGADESHVWNDLLHRKSSYWGMAANYTNQVTQKLQLKAGGDFQRHTLRYFDHYTPT
                     QAFDKNGNPDNVIDVDHYGYDALGNELNDGDTFTDTNGNNSYDEGEPFNDLNGNGIYD
                     DPLDAAKHPKVASAYVQGKFEQLGLVVNAGLRWDYLTPATHALKSETLPLGPEFGGVD
                     TTNNPTTLDPTDLEDSKVYQRLSPRLGIGFPVTDQTLLHVNYGQFFQQPNLQDLYASY
                     TFLEYKINNGGYFVGFGNPNLKPEETTAYELGIQHTPNERSRIDATIYYKDVKDLVEI
                     VTIRSSPNAFSSYRNTDFATIKGLDLAYTMRRTGHVSMNAAYSLSWARGTGSISQSQR
                     TIAWTAQEPPKLATPLSFDQRHRFTLNFDYRFGAGEGPTWGGTRWLENTGINVLFNAA
                     SGTPYTPTKVYNAVTLGATFPTPTGEINSQYGPWTATADLKASKAFGIGRGQNIEAYV
                     LVKNITNRKNVYAVYTATGSAESTNYLNTDAGQAAFSTPEDQELYHAAELNPDYFGNP
                     RQVRFGARLSF"
     gene            5422..>6058
                     /locus_tag="VFR25_02045"
     CDS             5422..>6058
                     /locus_tag="VFR25_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939861.1"
                     /translation="MHHRVRMAAVALVLGLVAVHPAIAAMKSGGATVTGSKKAIRKAA
                     KSLDNDDRMNVNNLDMFVTNHGSFGWDLATSQPGLRFPNGTLKTALFAAGIWVGAKVN
                     GEIRTAVAEYSQEFAPGPMYNGTFQVDNQSFRNYRLDAGNTTSPDYVNWPSAPPPAGQ
                     GAPLDSLGNPVVLGDATIWSVYNDAQASRHTNGSAGRTEPLGLEIQQTTFAY"
BASE COUNT         1268 a         1930 c         1874 g          986 t
ORIGIN      
        1 acccagttgg aggtcctccg gtatatcgcg acggaggagg agggtcgcga catggatcgc
       61 ctcaagacct ccgaggagaa gctcaccttc tggacgaatt tctggaaggc gagggacccc
      121 agcccggaaa ccccccaaaa cgaggcccgg gacgagttct acaagcgggt cacctacgcg
      181 aaccagcact tctcggcggg aacgcagggc tggaggaccg acatggggcg gatctacatc
      241 aagtatggcc agcccgatga ggtcgtgcgg aaccccttcc ggttcgaggg cccgcccgag
      301 gagatttggt actactaccg agcccgctac acgttcttct tcgtggatga gaagggtttc
      361 ggccagtacc agatcgacga ctcccgaagc agcaagatcg actagcccac cacccgctcc
      421 acccatgggg aatggccagc taagcccaag ggtcgcaatc gattatcatt ttctttgcaa
      481 attagcgttt gacaggcctg gaatcaggtg cctagtgtcc cgctcggcgt gatccaggcg
      541 gtcgtccgcc gttgaaacga gttgggcgac ttcgtcttgt ccagacagcg cacgacaact
      601 tgaacggaaa ggaggtggcg aaaaaggtcc aagcgagctg ggaatgctcc accggatctt
      661 cccgggccta acgccgaaca taagtgagca cggctcgggc gcgaccctgg atgggccagg
      721 gtccgacacc aaggaggcca aaccagtatg tttcagcgca agctgatcgt agtgacgtgt
      781 gcggtcgctc tactcgtgct ttcggcgggc gtgagcgagg cttcgctttc gcgagtcgag
      841 gggatgggtt tgggctcggt cccttactta agccaattca ctgacgacta cgtgaacatc
      901 tacccgtatc ccacgtcggt ggttcgtcaa aacaatctcg ttctcgccga gctcggtcac
      961 aacccggacg gtgacgtcaa tccggtcacc aaggagaccg agcagagtct caccatcatc
     1021 aagaacttca cgaatttcgg cgccatcgcg ttccagatgg agcagaacga caacaactcg
     1081 tcgttcccga acaatctgac caaccagcag atcgacgtga tctggggcaa gggcttcgac
     1141 aagctcgacc tcgctgttcg tctcgacctc accaactcga agatcgagat ctcggacaac
     1201 gccaataatt cggcggagtc ccatggcatc gacacgttcg gcggcgctct ggatccgtat
     1261 ccgttcggtg cgttcaaccc ggatgcgatc ctgagtgggc cgttcgagct caacacgttc
     1321 ggcgtgaccc cggcggtcgc gctccatctg tccaacgaca accgcgtcga ggcggccctc
     1381 accctgcgca agtacagcct cgatcgcagc atcaccaccg gcggcgtcgc cggcgagtcg
     1441 tgggaagaca acggcaacat gtcctatgca ctcttcgttc gcggcatcat gaacaagggc
     1501 gatcgcgcga cctggtaccc ggccgcgtgg tacgtgaacg acgacctcgg ctacacggtg
     1561 acgaactcgg gcatcggcgt cgagccccgc gacatcgacg agacgtacaa gacctggggt
     1621 gtcggtctct cccacaacat gcgcgtgaac gacaacaacc tgctcatctt cggagcctcg
     1681 gtcggcgagg cgaagcacga gtacgaccgc aacgactcga acgacggcgc gctcaacgcc
     1741 gagaccaaga cgttcgaaga gaagaccatg ctcctgccga tcttcttcgc cggtctcgag
     1801 accgaggcca cgagctggct gaagatccgg atgggcgcga cgcagtccct cgagaacaac
     1861 gagaccgaga cgacggcctt cgacgggacg tcgctcaaga tcaagacgca cgagtcgagc
     1921 ttcgacttca acctgggcgc cggtatccgg tggaacaacc tcgacttcga cctgacgctg
     1981 aacgaggcct tcccgatgtc gggcggctgg attctgtcgg gcgacgaggc gaccccggcc
     2041 acgcgcgctt cggcgacgta ccacttctag acccaaccgc ggcgggtttg accccgccac
     2101 gggagcagca aggccgcccg gcgaattccc gccgggcggc ccttttttgt gaaaaagtgt
     2161 tgacaccgct tgaaagcgga cttctactga accacagggc atggtgttgc ggcgccggtc
     2221 cggcgctgtc ggtgagcaca cacgcaaagt caggaggttc atcaagccgg tcaacgtgga
     2281 tgtgccttaa agggataccc ggtgtgtgac gatggagttg gcagggtcca gctcccgtgg
     2341 ggatgagggg acggatggcg gacgacactc agggatcgag tgacccttcg gaaggcgttc
     2401 cacagctttg gaggggaggg ccagtcatga cgcgacttcg cgtgcgccgc gggttaggcg
     2461 tagccacggt tttgatcgtg ggctgcgccc tttttgtttt tgggggcacg gcccgcgccg
     2521 gaaccgccgg cgggatcacg gggaccatcc gtgacgcggc cacgagcgag cccatcgggc
     2581 tcgcgacggt caccatcccc gagttgaagc ggggagccac gaccgacgct cagggtcact
     2641 atttcatcct caatctgctc cccggcaagt acaccgtccg ggtcgccttg ctcgggtaca
     2701 tccctcaagt acgcgagggc gtcgaggtgt tcccggactt caacgccaag gtcgatttca
     2761 ccatgacctc gacggtcctc aaggacgttc aggaggtcca ggtcaagggg gagcgtccgc
     2821 tcatccagaa ggacgtcacc accaccacca agttcttgaa tggcgacgag atcaagaacc
     2881 agccgctgcg cggctaccag gaggcggtgg cccagcaagc gggtgtggtc tcgttcaacc
     2941 tgaacaatct gaatcaggtg agcgccggga ccgagatcca gaacagcaac accctcatca
     3001 tccgcggtgg ccgccccaac gaggtggcct actacgtgga tggattctcg cagcaggacc
     3061 cgctcaccgg cttctcgacg acgtccatcg cgaatgacgc gatcgacgag gtcgtggtgc
     3121 agtccggcgg gttcaatgcc gagtacgggc gcatcaactc gggcatcgtg aacgtcgtga
     3181 cccgagaggg aggggatcgc tacgctggat ccgtcgaggg tcagctcggg gattacctcg
     3241 atcgccgcgg cgaccacacg atcctggcgg cgtcgctcgg cggtccgatc actccgaagt
     3301 tcaagaacct gaccttcttc ctctccggcg agcaccagaa catcgacgac cggaagccga
     3361 gcttcatcac ggatgagctg ttcctgcctc agcagcccgg cgtcttcgaa gacggcgtcc
     3421 tgccggccaa cgacagcgag tcgtgggcga ccgccgcgaa gatggcgtac aagctctcgc
     3481 cgaccaagac gatccgggtc ggcggcacct acaacaagga cgactggcgg cagttcttga
     3541 gctcctatcg gttcaacctg gcgcactcgc cgcgttacaa ggacgagaac tggtccgcgt
     3601 tcggttcctg gaaccacacg gtcaacaacc gatcgttcta cgagctccgg gcgaacatgt
     3661 tctcgacgga gcgcatccgt ggagacggca tctacttcga tgacctgagg ggctatgcga
     3721 ggcccgaagg caacccgtcc ttcgacgatc tggccctcta ctggaacggc gacgatcccg
     3781 caacgggcgc ggacgagtcg cacgtctgga acgacctcct gcaccggaaa tcgtcgtact
     3841 ggggcatggc ggcgaactac acgaaccagg tcacccagaa gctgcagctc aaggcgggcg
     3901 gcgactttca gcgccacaca ctgcggtact tcgatcacta cacgcccacc caggcgttcg
     3961 acaagaacgg caaccccgac aacgtgatcg acgtcgacca ctacggctac gacgcactag
     4021 ggaacgagct gaacgacggc gacaccttca ccgacacgaa cggcaacaac agctacgacg
     4081 agggcgagcc gttcaacgac ttgaatggga acgggatcta cgacgatccg ctggacgcgg
     4141 ccaagcaccc caaggtcgct tctgcgtacg tccagggtaa gttcgagcag cttgggctgg
     4201 tcgtgaacgc cggccttcgc tgggactatc tgacccccgc cacgcacgcg ctgaagagcg
     4261 agacgctccc cctggggccc gaattcggag gcgtggatac gacgaacaat ccgaccacgc
     4321 tcgatccgac ggacctcgaa gactcgaagg tgtaccagcg gctcagcccg cgcctcggaa
     4381 tcgggttccc ggtcaccgac cagacgctgc tccacgtgaa ctacgggcaa ttcttccagc
     4441 agccaaatct ccaggacctc tacgcgagct acaccttcct cgaatacaag atcaacaacg
     4501 gcggctactt cgtcggattc ggcaacccga atctgaagcc ggaggagacg acggcgtacg
     4561 agctgggcat ccagcacacg ccgaacgagc ggtcccgcat cgacgccacg atctactaca
     4621 aggacgtgaa ggatctcgtc gagatcgtca cgatccgatc gtcgccgaac gcgttctcgt
     4681 cgtaccggaa cacggacttc gcgacgatca agggtctcga cctggcgtac accatgcgcc
     4741 ggacggggca cgtgtccatg aacgcggctt atagcctctc gtgggcgcgc ggcaccgggt
     4801 cgatctcgca gtcgcagcgg acgatcgcgt ggacggccca ggagcccccg aagctcgcga
     4861 cgccgctctc gttcgaccag cggcatcggt tcaccctcaa cttcgactac cggttcggcg
     4921 cgggtgaagg cccgacctgg ggcggtacca ggtggctcga gaacaccggt atcaacgttc
     4981 tcttcaatgc cgcgagtggg acgccctaca cgccgaccaa ggtctacaac gcggtgacgc
     5041 tcggcgcgac gttcccgacg ccgacgggtg agatcaactc gcagtacggc ccgtggacgg
     5101 cgacggcgga tctcaaggcg agcaaggcgt tcggtatcgg ccggggccag aacatcgagg
     5161 cctacgtgct ggtcaagaac atcaccaacc ggaagaacgt ctacgcggtc tacaccgcga
     5221 cgggcagcgc ggagagcacg aactacctga ataccgatgc cgggcaggct gcgttcagca
     5281 cgcccgagga tcaagagctc tatcacgccg ccgagctgaa ccccgattac ttcggcaacc
     5341 cgcgccaggt gcgctttggc gcgcggctga gcttttagcg atggccacgg caaccgctgg
     5401 acgtccaagg agggacgaag catgcaccac agggtgagga tggcggccgt ggcgctggtc
     5461 ctcgggctcg tcgcggttca tcccgcgatc gcggccatga agagcggcgg cgcgaccgta
     5521 acgggatcca agaaagccat ccggaaggcg gcgaagagcc tcgacaacga cgaccggatg
     5581 aacgtgaaca atctggacat gttcgtcacg aaccacggct cgttcgggtg ggatctcgcc
     5641 acgtcgcagc ctggattgag gttcccgaac ggaaccctga agacggccct gttcgcggcg
     5701 gggatctggg tcggagccaa ggtcaacggg gagatccgga ccgccgttgc cgaatattcc
     5761 caggagttcg cgcccggccc catgtacaac gggaccttcc aggtcgacaa ccagtcgttc
     5821 cggaactacc ggctggatgc agggaatacg accagtccgg actacgtgaa ctggccgtcc
     5881 gctcctccgc cggctggtca gggcgcgccg ctcgactcgc tggggaaccc cgtggttctc
     5941 ggcgacgcga cgatctggag cgtgtacaac gacgcccagg cgtcccggca cacgaacggc
     6001 agcgcgggcc ggaccgagcc gctcgggctc gagatccagc agacgacgtt cgcgtaca
//
LOCUS       DASTKC010000048         9089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_745832, whole genome shotgun sequence.
ACCESSION   DASTKC010000048 DASTKC010000000
VERSION     DASTKC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745832"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFR25_02050"
     CDS             <1..1038
                     /locus_tag="VFR25_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9939862.1"
                     /translation="SGFGSCQGIAWRPDGKEIWFSATRGGTGAEIYAATLDGTVRKVA
                     GSPGGMRITDIAPDGRVLFVRDNHRAELHVLVPGATEERELTWGDWSVLADLSDDGHT
                     ILFTEEGDAVGGEYAVCIRGTDGSPVVKLGGGNAAALSPDGRYALATTLDPPTELLLF
                     PTGAGQARTLPRGPIRTHYQAAWLPDGSGVFSMGEDSTGTVKGYVQDVAGGDPRPVLQ
                     GVDFTAGSVHVAPDGRYAAVSPLHKLYSVTDGSSIDIPGLAPSERLYCAGFSRDGREI
                     FVSEIGSRRPTLFGIDLVTGTRRVIRVITPRSPAGVLSATLMRATGDAKTIAYGIDRN
                     LSEIYVATGLH"
     gene            1089..1895
                     /gene="kdsB"
                     /locus_tag="VFR25_02055"
     CDS             1089..1895
                     /gene="kdsB"
                     /locus_tag="VFR25_02055"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET9939863.1"
                     /translation="MPRPQTASADREGLSRSTQGAVAIIPARLHSTRLSEKALQDLGG
                     APLIVRVLERVREASCLRDVWVATDSERIAEAVRGAGGQAIMTRADHASGLDRVAEAV
                     GILSDAPAPDPDQVFVNVQGDEPFVSPAGLDHLVRLFESPDVVMGTLAAPFPAWASPD
                     DPNKVKLVRDRANRALYFSRSPIPSGMDPGAPRLLHVGVYAYRRRTLLELARMEPAPL
                     ERAERLEQLRALSNGIPIHVAVGDYESVGIDTPEDLDRARALWGEGRSRS"
     gene            1892..3541
                     /locus_tag="VFR25_02060"
     CDS             1892..3541
                     /locus_tag="VFR25_02060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9939864.1"
                     /translation="MTKFVFVTGGVVSSLGKGIAASAIGSLLKSRGLSVTLQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDVGHYERFLGVSMGRQNNVTAGQIYDTIIQKE
                     RRGDYLGRTVQVIPHVTDEIKSRMLALTRGKKPVDVAIVEIGGTVGDIESLPFLEAIR
                     QLRLELSTVNTLFIHVTLVPHLGAAREIKTKPTQHSVKELRAIGIQPDILLARSEVPI
                     PQEVKEKIGLFCNVPTQAVIEAIDVSSIYEVPLMFHRGGLDDLVVDMLRLDAGPADLS
                     AWQRYQERVKAARDSVTISVVGKYTHLRDAYKSISEAITHGGAANGVSVKIDWVDSER
                     IEMDGPDALLGSSHGILIPGGFGDRGTQGMVLAARYARERRVPFFGICLGMQCAVIEM
                     ARDLAGLDGADSSEFDPETPHPVIALLESQHGVTKKGGTMRLGAYRCVVAPGSRASEA
                     YGASEVSERHRHRFEFNNDYQAPLEAKGMRVTGVYPELSLVEIVELPDHPWFVGVQFH
                     PELRSRPDQPHPLFQGFIRAANEERGKRTGTSDSRAGAARN"
     gene            3543..4391
                     /gene="kdsA"
                     /locus_tag="VFR25_02065"
     CDS             3543..4391
                     /gene="kdsA"
                     /locus_tag="VFR25_02065"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET9939865.1"
                     /translation="MDARFQPIAVGSVRIEPGRLALIAGPCVLEDDTMAEEVAQEVKR
                     IAVSLGMPFVFKASFAKANRSSRESYRGPGAERGLASLARIRERVGVPVTSDVHETSD
                     VSDAARALDLLQIPAFLCRQTALLEAAGASGKPVHVKKGQFLDPASMGRAVEKVRAAG
                     APGVIVTERGTTFGYGDLVVDMRSIPTMARLGCPVFFDATHSVQRPGGVETGGQREFI
                     PALARAAVAAGADGLFIETHPDPARARSDRESQWPLGDLEPLLRSLMKVHQSVADPSL
                     VETRRT"
     gene            4400..5410
                     /locus_tag="VFR25_02070"
     CDS             4400..5410
                     /locus_tag="VFR25_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET9939866.1"
                     /translation="MEVEPASKAASPSGSVTAWAREVLDLEGEAILALKPRIGPEFEA
                     AVHAVAAVKGQVLALGVGKSGLVAKKIAATLTSTGTPATFVHPADAAHGDLGIVSRDD
                     AAILLSKSGETAELLELLPALRRHGVLVVTITCHPTSSLARGADHVLDVGRPREAGPE
                     DLVPTATTTAMLALGDALAIVLMRLKGFTREDFAYLHPGGVLGRVALLRVADRMHTGT
                     ALPRVPETATLHEALVEILNKRLGMTTVVDGEGVLLGILTDGDLKRILLQGSADLSLP
                     VSRVMSRSPRTIEKEALIAQAVRSMEENPGGAITSLVVLDGLGRPDGVLHLHDCLDLR
                     AR"
     gene            5463..6002
                     /gene="lptC"
                     /locus_tag="VFR25_02075"
     CDS             5463..6002
                     /gene="lptC"
                     /locus_tag="VFR25_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET9939867.1"
                     /translation="MLVAALAMGCHKDAPSTTSGEEVRVPDQEARDFTLTESMEGKKS
                     WTLWASYAAMFNDKSLVDAKTVRIDFFDKEGKKNSSLTADQGLVFQRTNDLEARGKVR
                     VTTESGVTLETDSLRWQNASGKIVSDAFVRVTRNGDVVTGYGFESDANLEHFHLAREV
                     RAEVQPQAGENPDSVGSLR"
     gene            5999..6724
                     /gene="lptB"
                     /locus_tag="VFR25_02080"
     CDS             5999..6724
                     /gene="lptB"
                     /locus_tag="VFR25_02080"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET9939868.1"
                     /translation="MSGLVARDLERYYGHWKVVNKVTVQVDRGQVVGLLGPNGAGKTT
                     TFYLIVGLLRADGGQISIDGRDITSLPMHQRARIGIGYLAQEPSIFRRLTVRENILAI
                     LETLKLSSKERAQRLEQLLEELNIAHLADRKGYNLSGGERRRVEITRALVTQPGFLLL
                     DEPFVGIDPIAVAEIQDIVSRLRQKGLGILITDHNVRETLTTTDHAYIMFEGKILMQG
                     TGQELAQDPVARQIYLGERFRLD"
     gene            6743..8170
                     /gene="rpoN"
                     /locus_tag="VFR25_02085"
     CDS             6743..8170
                     /gene="rpoN"
                     /locus_tag="VFR25_02085"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET9939869.1"
                     /translation="MKHGLSMQQKPTLIMTQRLQQALKLLQMPTLELQQALKLELERN
                     PLLEEVDEIEEVQEIEEVKKEEGQDGEAEPVETAEPDKNQEVDWNELWPDNYEIPSAP
                     RTDDPDAEFYERVPVTRRSLGDHLIEQLRLLERGEEDLAIGEYLIGSIDENGYLQTTV
                     EEVAEAFAVAPEKVEEVLAEIQKFEPAGVGARNLQECLWIQLVQRKDEGTLAGRVVQE
                     QFDNLLAKRFGEIARGLKCSIEEVQAAGDLIATLDPRPGQEIAAEETKYVTPDLIVER
                     VAGEFVVSLNDRNVPRLRISQAYQQMLRNKSAGQDETRKFISDKLNSAKWLIQTIEQR
                     RKTMIKVMRRIVEEQREFFERGVEGLKPLTLQQIANQIGMHESTVSRVTTNKYVQTPR
                     GVFELKYFFSSGLATEEGDDVSAKVAKDKILTLINNEDKMDPLSDQRIAEILHEQGLR
                     IARRTVAKYREALRLLPARARRRYA"
     gene            8196..8723
                     /gene="raiA"
                     /locus_tag="VFR25_02090"
     CDS             8196..8723
                     /gene="raiA"
                     /locus_tag="VFR25_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9939870.1"
                     /translation="MEITIKGRHWKPTPGFREYAVERLERLTRFYPAIIRADLVVTQE
                     GYRHSAELRLHGNALDLLTKATDSEAKASLDQVLTKVERALQKQKDKLKDRKKRGASI
                     RMEPAVETPPRAKAAIEVVRLRPPKRPVLSAEEAARALMKSRKPVLVFAERGEEGLRV
                     AYRLEDGQVGLLELD"
     gene            8710..>9089
                     /locus_tag="VFR25_02095"
     CDS             8710..>9089
                     /locus_tag="VFR25_02095"
                     /inference="COORDINATES: protein motif:HMM:NF014642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET9939871.1"
                     /translation="MSSTSPPTLTVAQLYEQQKDQLSLEHLSESLESKVPITMSDINR
                     PGMALMGFTENFLAERIQIFGRTELAYLGTLKDKERRAALDRFFQFQAPCIIVAKGLE
                     IPSELVGRANDHEIPVFRTPQDTTP"
BASE COUNT         1564 a         3006 c         3149 g         1370 t
ORIGIN      
        1 tcgggattcg ggagctgcca gggcatcgca tggcgccccg atggaaaaga gatctggttc
       61 tcagccaccc gaggcggcac cggggcagag atctacgcgg ccacgctgga cgggacggtc
      121 cggaaggtgg cggggagccc cggaggcatg cggatcaccg acatcgctcc cgatgggcga
      181 gtactcttcg tccgggacaa ccaccgggcg gagctccacg tgctcgtgcc tggtgcaacc
      241 gaggagcgcg agctcacgtg gggagactgg tccgtcctcg cggatctgag cgatgatggc
      301 cacacgattc tcttcacgga agaaggagac gcggtgggtg gcgagtacgc ggtctgcatc
      361 cggggaacgg acgggtcgcc ggtcgtgaag ctggggggcg ggaacgcggc ggcactgtcg
      421 ccagacggtc ggtacgccct cgcgacgacg ctcgatcccc cgacggagct cctcctcttt
      481 cccacgggag cggggcaggc caggacgctt ccgcgcgggc ccattcgaac tcactatcag
      541 gctgcgtggc tccccgacgg cagcggagtc ttctccatgg gggaggactc gactgggacc
      601 gtgaaaggct acgtccagga tgtcgcggga ggcgatccgc gcccggtcct ccagggcgtg
      661 gatttcacgg cggggagcgt acatgtggcc ccggacggcc gctacgccgc tgtgtccccc
      721 ctccacaagc tctactccgt gacggatggc tcctccatcg acataccggg actcgctccc
      781 agcgagcgct tgtactgcgc cggtttcagc cgcgatggcc gggagatctt cgtgtccgag
      841 atcggatccc gacggccgac gcttttcggg atcgacctcg ttaccggaac ccggcgcgtc
      901 atccgggtca tcacgccgcg aagtcccgcc ggcgttctca gcgccacctt gatgcgcgcc
      961 acgggggatg cgaagaccat cgcctacggg atcgatcgca acctttccga gatctacgtc
     1021 gcgacgggac ttcattaggg atcccgccgc ctcttgacgc gccccacacg cgttggatag
     1081 catggctcgt gccccgtccg cagaccgcct ccgcggatcg cgagggcctc tcgcggtcca
     1141 cccaaggcgc cgtggccatc atcccggcgc gactccactc cacccgtctc tctgaaaagg
     1201 ccttacagga tctcggcggc gctcctctga tcgtccgcgt gctcgagcgc gtgcgggagg
     1261 cgtcgtgcct cagggacgtg tgggtggcga cggacagcga acggatcgcg gaggccgtcc
     1321 gcggcgcggg ggggcaggcg atcatgacgc gcgccgatca cgcgtccggg ctcgaccgcg
     1381 tggcggaagc ggtggggatt ctctcggatg ctccggcgcc cgatcccgac caagtcttcg
     1441 tcaacgtgca aggcgacgag ccgttcgtga gccccgctgg cctcgatcat ctcgtccggc
     1501 tcttcgagag tccggacgtg gtgatgggga ccctcgcggc gccgtttccg gcgtgggcgt
     1561 ctccggacga tccgaacaag gtgaagctgg tgcgcgaccg cgcgaatcgc gcgctctact
     1621 tctcgcgctc gcccattccc tcggggatgg atccgggcgc gccacgcctt cttcacgtgg
     1681 gcgtctacgc atatcgccgg cgcacgctcc tcgagctcgc gcgcatggag ccggcgccgc
     1741 tcgagcgtgc ggagcggttg gagcagcttc gtgcgctgtc gaatggaatt ccgatccacg
     1801 tcgccgtggg cgactacgaa tccgtgggaa tcgatacgcc cgaggatctg gaccgtgctc
     1861 gggccctctg gggggaaggg aggtcgcgat catgaccaag ttcgtgttcg tcacgggcgg
     1921 agtcgtctcg tcgctcggaa agggaatcgc cgcgtccgcc atcggctcgc tgctgaagag
     1981 ccgcgggctc tcggtgacgc tccagaagtt cgatccctac ctgaacgtcg atcccgggac
     2041 gatgagtccg taccagcatg gtgaagtgtt cgtgaccgac gacggcgccg agacggatct
     2101 cgacgtgggt cactacgagc gcttcctcgg cgtctcgatg gggcgccaga acaacgtcac
     2161 cgcgggccag atctacgaca cgatcatcca gaaggagcgc cgcggcgact atctcggccg
     2221 tacggtgcag gtgatcccgc acgtcacgga cgagatcaag tcgcgcatgc tcgccttgac
     2281 gcgcgggaag aagcccgtgg acgtggcgat cgtcgagatc gggggcacgg tgggcgacat
     2341 cgagagcctt ccgttcctcg aggcgatccg ccagctcagg ctcgagctct ccaccgtgaa
     2401 cacgctcttc atccatgtga ccctcgtgcc gcacctgggc gccgcgcggg agatcaagac
     2461 caagccgacg cagcactccg tgaaggagct ccgcgcgatc ggtatccagc ccgacatcct
     2521 cctcgcccgc tccgaggtgc cgattccgca agaggtgaag gagaagatcg ggctcttctg
     2581 caacgtgccg acgcaggccg tgatcgaggc gatcgacgtc tcgtcgatct acgaggtccc
     2641 gctcatgttc caccggggcg gcctggacga cctcgtcgtc gatatgctgc gcctcgacgc
     2701 ggggcccgcg gacctctccg cgtggcagcg gtaccaggag cgcgtcaagg ccgcacgcga
     2761 ctcggtcacg atctccgtcg tgggcaagta cacgcatctc agggacgcgt acaaaagcat
     2821 ctcggaggcc atcacgcacg gcggcgccgc gaacggcgtg tcggtgaaga tcgactgggt
     2881 cgattccgag cggatcgaga tggacggtcc cgacgcgctc ctcggctcgt cccacggcat
     2941 cctgatcccg ggcggcttcg gcgaccgtgg cacccagggg atggtgctcg cggcgcggta
     3001 cgcgcgggag cgccgggtgc cgttcttcgg aatctgtctc ggcatgcagt gcgccgtgat
     3061 cgagatggcc cgcgacctgg cggggctcga cggagccgac tcctcggagt tcgaccccga
     3121 gacgccgcac ccggtgatcg cccttctcga gtcgcagcac ggcgtcacga agaagggggg
     3181 cacgatgcgc ctcggcgcct accggtgcgt cgtcgcgccg ggaagccgcg cctccgaagc
     3241 gtacggcgcg agcgaggtgt ccgagcggca ccggcaccgg ttcgagttca ataacgacta
     3301 ccaggccccg ctcgaggcga agggcatgcg cgtgaccggc gtctaccccg agctctcgct
     3361 cgtcgagatc gtggagctgc ccgaccatcc ctggttcgtc ggcgtgcaat tccatccgga
     3421 gctcaggtcc cgtccggatc agccgcaccc gctgttccag ggcttcatcc gcgcggccaa
     3481 cgaggagcgc ggcaagagga ccggcacctc cgactcgcgc gcgggcgcgg cgcggaactg
     3541 acgtggacgc gcggttccag cccatcgcgg tcggatcggt ccggatcgag cccggccgtc
     3601 tcgcgctcat cgcgggaccc tgcgtgctcg aggacgacac gatggccgag gaagtcgcgc
     3661 aggaggtgaa gcggatcgcg gtctcgctcg ggatgccctt cgtcttcaag gcctcgttcg
     3721 cgaaggcgaa ccgctcctcg cgggagagct accgcggtcc cggcgcggag cgcggcctcg
     3781 catcgctcgc ccggatccgg gagcgagtcg gcgttccggt gacgagcgac gtgcacgaga
     3841 cctcggacgt gtccgacgcg gcgcgcgcgc tcgacctcct ccagatcccg gcgttcctct
     3901 gccggcagac cgcgctcctc gaagcggccg gtgcgagcgg gaagccggtg cacgtgaaga
     3961 aggggcaatt cctcgatccg gcgtcgatgg ggcgcgcggt cgagaaggtg cgcgccgcgg
     4021 gcgcgccggg cgtgatcgtg acggagcgag ggaccacttt cggctatggc gatctcgtcg
     4081 tggacatgcg ctcgatcccg acgatggccc ggctcggctg cccggtcttc ttcgacgcga
     4141 cgcactcggt gcagcgcccc gggggcgtgg agaccggagg gcagcgggag ttcatccccg
     4201 ccctcgctcg ggcggcggtg gccgcggggg cggacggcct tttcatcgag acccatcccg
     4261 atcccgcacg ggctcgatcc gaccgcgagt cacagtggcc gctcggggac ctggagccgc
     4321 ttctccgaag tctcatgaag gttcaccaat cggttgccga tccatctctt gtggagacga
     4381 gacgcacgtg aagcgaagcc tggaagtgga accggcgtcg aaggcggcga gcccgagcgg
     4441 ctcggtcacg gcctgggcac gcgaggtcct ggatctcgaa ggggaggcca tcctcgcgct
     4501 caagccgcgg atcggtcccg agttcgaagc cgccgtccac gcggtcgcgg cggtgaaggg
     4561 gcaggtcctc gcgctcgggg tcggaaagtc gggactcgtg gcgaagaaga tcgcggcgac
     4621 cctgacgagc accggaacgc ccgcgacgtt cgtgcatccg gccgacgcgg cgcacggcga
     4681 cctcgggatc gtgtcgcgcg acgacgccgc gatcctcctg tcgaagagcg gcgagacggc
     4741 ggagctgctc gagctcctgc cggcgctccg tcgccatggc gtcctcgtcg tcacgatcac
     4801 gtgccatccc acatcctcgc tcgcgcgcgg agcggaccac gttctcgatg tcggccgtcc
     4861 gcgtgaggcg ggccccgagg acctggtccc gaccgccacg accaccgcga tgctcgcgct
     4921 cggggacgcg ctcgcgatcg tgttgatgcg gctcaaggga ttcacgcggg aggacttcgc
     4981 ctatctccac ccgggcggcg tcctcgggcg cgtcgcgctc ctgcgcgtcg cggaccgaat
     5041 gcacacggga acggcgctcc cccgcgtgcc cgagaccgcc acgctccacg aggcgctggt
     5101 cgagatcctg aacaagaggc tcggaatgac gaccgtggtc gacggcgagg gcgtgctctt
     5161 gggcatcctc acggatggcg acctgaagcg gatcctgctc caggggagcg cggacctctc
     5221 gctccccgta tcccgcgtca tgtcccggtc gccccggacc atcgagaagg aggcgctgat
     5281 cgcccaggcg gtccgctcga tggaggagaa tcctggcggg gccatcacct ccctggtcgt
     5341 tctggacggc ctcggccgcc cggatggggt gctccacctg cacgattgcc tcgaccttcg
     5401 ggcgaggtaa gaccatgcgg ttccaatggt ttgaccgtgg cttcgcgggc gtggtactgt
     5461 ccatgctagt cgcggccctg gccatgggct gccacaaaga tgcgccctcc acgacgagcg
     5521 gggaggaggt ccgggttccg gaccaggaag cgagggactt cacgctcaca gagtccatgg
     5581 agggaaagaa gagctggacg ctctgggcgt cgtacgcggc gatgttcaac gacaagagtc
     5641 tcgtcgacgc caagacggtc cggatcgact tcttcgacaa ggaagggaag aagaactcgt
     5701 ccctgaccgc tgatcagggg ctggtcttcc agagaacgaa cgacctcgag gcgcggggca
     5761 aggtgcgcgt cacgaccgag agcggcgtga cgctcgagac cgactcgctc aggtggcaga
     5821 acgcgagtgg caagatcgtc tccgacgcct tcgtgcgcgt gacccgaaac ggggatgtgg
     5881 tgacggggta cggcttcgag agcgacgcca atctcgagca tttccacctc gcgcgcgagg
     5941 tgcgcgccga ggtccagccc caggcgggcg aaaatcccga ctcggtgggg tcgctgcggt
     6001 gagcggtctc gtggcgcggg acctggagcg ctactatgga cactggaagg tggtcaacaa
     6061 ggtcaccgtc caggtcgacc gcggccaggt cgtgggcctc ttggggccga acggggcggg
     6121 gaagaccacg acgttctacc tgatcgtcgg gctcttgcgc gcggatggcg ggcagatctc
     6181 gatcgacggg cgggacatca cgtcgcttcc catgcaccag agggcccgga tcggaatcgg
     6241 gtacctggcg caggaaccgt cgatcttccg tcgcctgacc gtccgcgaga acatcctcgc
     6301 gatcctcgag acgctgaagc tctcctcgaa ggagcgggcg cagcggctcg agcagctcct
     6361 cgaggagctg aacatcgcgc acctggcgga ccggaagggc tacaacctgt cgggaggcga
     6421 gcggcggcgc gtggagatca cccgcgcgct cgtcacgcag ccgggctttc tcctcctcga
     6481 cgagccgttc gtcgggatcg acccgatcgc cgtcgcggag atccaggaca tcgtgtcccg
     6541 cttgcgacag aaggggctgg gcatcctcat cacggaccac aacgtacgcg aaacgctgac
     6601 cacgacggac cacgcgtaca tcatgttcga aggcaagatc ctgatgcagg gcaccggcca
     6661 ggagctcgcg caggatccgg tcgcgcgcca gatctacctg ggcgagcgct tccgactgga
     6721 ttgaggggct agagccatgg aaatgaaaca cggcctgagc atgcagcaga agccgacgct
     6781 gatcatgacg cagcgtctgc agcaggcctt gaagctcctc cagatgccga cgctcgagct
     6841 ccagcaggcg ctgaagctgg agttggagcg gaatcctctc ctcgaggaag tggacgagat
     6901 cgaggaggtg caggagatcg aggaggtcaa gaaggaggag ggccaggacg gcgaggcgga
     6961 gccggtcgag accgcggagc cggacaagaa ccaggaagtc gactggaacg agctctggcc
     7021 cgacaactac gagatcccgt cggccccgcg caccgacgac cccgacgccg agttctacga
     7081 gcgcgtcccc gtcacgcggc gcagcctggg ggatcatctc atcgagcagc tcaggctcct
     7141 cgagcgcggc gaagaggatc tcgccatcgg cgagtacctg atcggctcga tcgacgagaa
     7201 cggctatctc cagacgacgg tcgaagaagt ggccgaggcg ttcgccgtgg cgccggagaa
     7261 ggtcgaggaa gtgctcgcgg agatccagaa gttcgagccc gcgggagtcg gcgcgcggaa
     7321 tctccaagag tgcctgtgga tccagctcgt gcagcggaag gatgaaggaa cgctcgcggg
     7381 tcgggtcgtg caggagcagt tcgacaatct cctcgccaag cgcttcggtg agatcgcgcg
     7441 cggcctcaag tgctcgatcg aggaggtgca ggcggcgggg gatctcatcg cgaccctgga
     7501 cccgcggccg ggacaggaga tcgcggccga ggagacgaag tacgtcaccc ccgacctgat
     7561 cgtcgagcgc gtggcgggcg aattcgtggt atcgctcaac gaccggaacg ttccgcggct
     7621 tcggatcagc caggcctacc agcagatgct ccgcaacaag agcgccgggc aggacgagac
     7681 gcggaagttc atctcggaca agctcaactc cgcgaagtgg ctcatccaga cgatcgagca
     7741 gcggcgcaag acgatgatca aggtgatgcg ccggatcgtc gaggagcagc gggagttctt
     7801 cgagcgcggc gtggaggggc tgaagccgct caccctccag cagatcgcca atcagatcgg
     7861 catgcacgaa tcgaccgtga gccgcgtcac gaccaacaag tacgtccaga cgccgcgcgg
     7921 cgtcttcgag ctgaagtact tcttctcgag cggcctcgcg accgaggaag gggacgatgt
     7981 ctccgccaag gtcgcgaagg acaagatcct gaccctgatc aacaacgagg acaagatgga
     8041 cccgctcagc gatcaacgca tcgccgagat cctgcacgag cagggactcc gcatcgcgcg
     8101 gcgcaccgtg gcgaagtacc gggaggcgct ccgcctcctg ccggcccgcg cgcggcgccg
     8161 gtacgcctga gcgcgtctta cccatagggg gtggcatgga gatcacgatc aagggacgtc
     8221 actggaagcc cacgcccggg ttccgggaat acgcggtcga gcgtctcgag cggctgacgc
     8281 ggttctatcc ggcgatcatc cgcgccgatc tcgtcgtcac gcaggagggc taccgccact
     8341 cggccgagct tcgcctccac gggaatgcgc tcgacctcct caccaaggcg accgacagcg
     8401 aggccaaggc ctcgctcgac caggtcctga cgaaggtgga gcgggcgctc cagaagcaga
     8461 aggacaagct caaggaccgg aagaagcgcg gcgcgagcat ccgcatggag cccgcggtcg
     8521 agaccccgcc gcgcgccaag gccgcgatcg aggtggtccg gctccgcccg cccaagcggc
     8581 ccgtcctgag cgcagaggag gccgcccgag cgctcatgaa gagccggaag cccgtcctcg
     8641 tcttcgccga gcgcggcgag gaagggcttc gcgtggccta ccgcctcgag gatggacagg
     8701 tggggctgct tgagctcgac tagcccgccg accctcacgg tcgcgcagct ctacgagcag
     8761 cagaaggacc agctctccct cgagcacctc tcggagtcgc tggagtccaa ggtcccgatc
     8821 acgatgagcg acatcaaccg tccggggatg gcgctcatgg gcttcaccga gaacttcctc
     8881 gccgagcgca tccagatctt cgggcgcacg gagctcgcct acctgggtac cctcaaggac
     8941 aaggagcggc gtgccgccct ggaccggttc ttccagttcc aggcgccctg catcatcgtc
     9001 gccaagggcc tcgagatccc gtccgagctg gtgggacgcg ccaacgacca cgagatcccg
     9061 gtgttccgca cgccccagga cacgacgcc
//
LOCUS       DASTKC010000049         5597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1455718, whole genome shotgun sequence.
ACCESSION   DASTKC010000049 DASTKC010000000
VERSION     DASTKC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455718"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFR25_02100"
     CDS             complement(<1..296)
                     /locus_tag="VFR25_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9939872.1"
                     /translation="MPVDVVLERADSARAEGESRTLADLMPRGPGPYADTLGLAGWSR
                     PLLDLGVRIAALAPSPATVFVHGETGTGKELIARALHEMSPRRERPFLPHNFAA"
     gene            complement(312..1331)
                     /gene="purM"
                     /locus_tag="VFR25_02105"
     CDS             complement(312..1331)
                     /gene="purM"
                     /locus_tag="VFR25_02105"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET9939873.1"
                     /translation="MSRYEEAGVNIKKGEEAVERIKAHLRSTFVPGVVGDVGAFAGAF
                     RIPEGGGQILLSSIDGVGTKLKVAILCDRHDTVGYDLVCHSANDILVHGGRPLFFLDY
                     VAMGVLEPERVEQLVAGLARGCREVGCALIGGETAEMPGLYREGDYDLAGAIIGIVEE
                     SGLVDGSRVAPGDVLLGLPSVGLHTNGYSLARTILFDRLKLRADDPMPGGGGTVGDVL
                     LSPHPWYGREVAVAREVGDVRAMAHITGGGLPGNLIRVLPEKCRADIRVGSWPVPFVF
                     RALVDAGGVPQDEAHRVWNMGIGFVLVVKRADADAVENALQAKGHDAHRIGEIVAGER
                     EVRLL"
     gene            complement(1328..2692)
                     /locus_tag="VFR25_02110"
     CDS             complement(1328..2692)
                     /locus_tag="VFR25_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9939874.1"
                     /translation="MMTKQRSIRSPRAWASRVLWTVVPSFFAALPILIALFGGPELHA
                     SRWDWPAIAVWAVLLFAAEALPVPLPRGGTMTIASILDIAALLLFGPWIAAALDLVTT
                     IPAQMLILRNRTGTAALSAGLYASTTIVAGAAYLAAGGALGAPRFPQDLLPLLLAGSL
                     YYALNTGWVSLVLGSQVREPAITIWRQQFQDGLAQYALSIGYGLLFAVVCMTAGLPGV
                     LLLVLPLLFARYALRLYADLRQDHVSFVRALSLALDAVDPYTHEHSVRVADYSVRVAR
                     RMGLPESEVETIRYAALVHDIGKIAQRPDVIRKPDTLDEEERRLMMRHPEAGARIIGQ
                     VRALGDAAKMVRTHHWRPDGRGYPPGLTEADVPVGARVIHVCDAFDAMVSDRPYRKGL
                     PVARALGELTRHAGTQFDAEIVFALVALHEEGRLMTEPDAARAEHAPADDPDRLEAFA
                     GDLP"
     gene            complement(2894..3823)
                     /locus_tag="VFR25_02115"
     CDS             complement(2894..3823)
                     /locus_tag="VFR25_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9939875.1"
                     /translation="MSIAVVGSIGLDTIQTPAGRVTEVLGGSAAYFSLAARHFMPVSL
                     VAVVGKDFPREARALLDQPDVDLAGLEIQDGKTFRWEGVYSDDMNTRETIRTELNVFE
                     RFKPDLPERTRSLKNVFLANIDPVLQREVLEQLDQPRLVLADTMNYWIRTKKDELLET
                     LKKVRIVIINEEEARELTGEALTHKAARAILSLGPETVVIKQGEHGAFLQGPEVFFTC
                     PAYPIDNVIDPTGAGDSFAGGFVGALARMARVNDHNLRRAVVYGCVLASFTVEGFGVS
                     GLAKADRDSILARAEAISDMTKFNLSDTARILR"
     gene            complement(3827..5506)
                     /gene="argS"
                     /locus_tag="VFR25_02120"
     CDS             complement(3827..5506)
                     /gene="argS"
                     /locus_tag="VFR25_02120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9939876.1"
                     /translation="MLLVKTWPIEAYLVDRVEDAVSKTGLTPPGGARVEIEVPRDPDH
                     GDWSTNVALTLAKTAKRPPREVAKALVDALEMDAAVIAAVEIAGAGFINFRLAPAWLQ
                     ETVRRVLGDPDGYGGSDAGKGERILVEYVSANPTGPLNIVSARAASLGDTLLRLFNAT
                     GHRAEGEFYVNDAGLQAELFGASVRTRFQERIGVPADAIPEEGYQGAYVADLAAALDE
                     AEGRAWLALPEREQRARFGRWGIDKNLEIQRHDLERFGARMDRYFLESSLHAGGAVQD
                     ARAMLEKRGVLDEREGAVWFRSTEFGDQEDRVIVRSNGEPTYFLADTAYHHDKFTRGF
                     DRLIDILGPDHHGHVQRMQGVLDALGWEKDRFEVIVAQWVRLLRGGETVKMSKRGGEF
                     ITLEELVEEVGVDAARFFFLMRRAESPMDFDLELAVKRSEDNPVYYVQYAHARIAHVL
                     DYARAQGVPEPDPERADLALLAEPETLTLLRGLAAFPSLVLASARSREPHRIPAFLKD
                     LAAKFHSFYHQHRVVTSDAPLTAARLLLTKATGAVFRRGLGLLGVSAPEAM"
     gene            complement(5478..5591)
                     /locus_tag="VFR25_02125"
     CDS             complement(5478..5591)
                     /locus_tag="VFR25_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HET9939877.1"
                     /translation="MLLDYVDVVVHVFHARAREYYLLDKLWGDAAREDVAD"
BASE COUNT          877 a         1940 c         1957 g          823 t
ORIGIN      
        1 atggcggcga agttgtgcgg gaggaaggga cgttcgcggc ggggactcat ctcgtggagc
       61 gcgcgagcga tcagctcctt cccggtgccg gtctcgccgt gcacgaagac cgtcgcgggg
      121 ctcggcgcga gggccgcgat gcggacaccg agatcgagga gaggccggct ccagccggcg
      181 aggccgagcg tgtccgcgta cgggcccggc ccgcgcggca tcagatcggc cagggtgcgc
      241 gactcccctt ccgcccgggc ggaatccgca cgctcgagca ccacgtccac cggcatctcc
      301 gacccccagc cctagaggag cctaacctcc cgctcccccg cgacgatctc accaatccgg
      361 tgcgcgtcgt gccccttggc ttggagcgcg ttctcgaccg cgtccgcgtc cgcacgcttc
      421 acgacgagca cgaatccaat tcccatgttc cagacgcggt gcgcttcatc ttggggcacg
      481 ccccccgcgt cgacgagcgc gcggaagacg aagggcacgg gccaggaacc gacacgaatg
      541 tccgctctgc acttctccgg gaggacgcgg atcaggttcc ccgggaggcc gcctccggtg
      601 atgtgggcca tcgcgcggac gtcgccgacc tcgcgtgcca cggccacctc ccgcccgtac
      661 caggggtgcg ggctcaagag aacgtccccc accgtgccgc cgccgccggg catgggatcg
      721 tcggcgcgga gcttcagccg gtcgaagagg atcgtgcgtg cgagcgagta gccgttcgta
      781 tggaggccca cgctcgggag accgaggagc acgtccccgg gcgccacgcg cgatccatcc
      841 acgagccccg attcctcgac gatgccgatg atcgcccccg cgaggtcgta atcgccttcg
      901 cggtagagcc ccggcatctc ggcggtctcg ccgccgatga gcgcgcaccc gacctcgcgg
      961 cagccgcgcg ccagtcccgc cacgagctgc tccacgcgct ccggctccag aacacccatc
     1021 gcgacgtagt cgaggaagaa gagcggacgg ccgccgtgca cgaggatgtc gttggctgaa
     1081 tggcagacga ggtcgtagcc gaccgtgtcg tggcggtcgc aaagaatcgc caccttgagc
     1141 ttggttccga caccgtcgat ggaggagagg aggatctgtc ctccgccttc gggaatgcga
     1201 aacgctcccg cgaacgcgcc gacgtcgccg accacgccgg gcacgaacgt cgagcgcagg
     1261 tgcgccttga tgcgctcgac ggcctcttcc cccttcttga tgttgacccc ggcttcctcg
     1321 tagcggctca tggaagatcc cccgcgaagg cctcgaggcg gtcgggatca tccgcgggcg
     1381 cgtgctccgc gcgcgccgcg tccggctccg tcatcagccg tccctcttca tggagcgcca
     1441 cgagcgcgaa gacgatctcc gcgtcgaact gcgtgcccgc gtggcgcgtg agctccccga
     1501 gggcgcgggc cacgggcagc cccttccggt acgggcggtc ggagaccatc gcgtcgaacg
     1561 cgtcgcagac gtgaatgacg cgcgccccga ccggcacgtc cgcttccgtg agccccggcg
     1621 gatagccgcg tccgtcggga cgccagtggt gcgtgcggac catcttggcc gcgtcgccga
     1681 gcgcgcggac ctgcccgatg atccgggcgc ccgcctccgg gtggcgcatc atgagacgcc
     1741 gctcctcctc gtcgagcgtg tccggcttgc ggatcacgtc ggggcgctgc gcgatcttgc
     1801 cgatgtcgtg gacgagcgcg gcgtaccgga tggtctcgac ctccgactcc ggcaggccca
     1861 tgcgccgggc gacgcgcacg gagtagtcgg cgacgcggac ggagtgttcg tgcgtgtacg
     1921 gatcgacggc gtcgagcgca aggctgagcg cgcgcacgaa gctcacatgg tcctggcgga
     1981 gatcggcgta gaggcggagc gcgtagcgcg cgaagaggag cggaagcacg aggaggagca
     2041 cgcccgggag ccccgctgtc atgcacacga ccgcgaagag caggccatag ccgatcgaga
     2101 gcgcgtactg cgcgagcccg tcctggaact gctgccgcca gatggtgatc gctggctcgc
     2161 gaacctgcga tcctaggacg aggctcaccc agcccgtgtt gagggcgtag tagagcgagc
     2221 cggcgaggag cagcggcagg aggtcttgag gaaagcgggg cgctcccagg gcgcctcccg
     2281 ccgcgaggta cgccgcgccg gcgacgatgg tggtcgacgc gtagagcccg gcgctcaagg
     2341 ccgcggtgcc cgtccggttg cgcaagatga gcatctgggc cggaatcgtg gtgacgaggt
     2401 cgagcgcggc cgcgatccag ggcccgaaga gaaggagcgc ggcgatgtcg aggatcgagg
     2461 cgatcgtcat cgtgccgccg cgcggaagcg gcacgggcag cgcctcggcg gcgaagagga
     2521 gaacggccca gaccgcgatc gcgggccagt cccagcggct cgcgtgcagc tccgggccgc
     2581 cgaagagcgc gatcaggatc ggaagggccg cgaagaacga gggcacgacc gtccagagga
     2641 cgcgggacgc ccaggctcgc ggtgaacgga tggagcgttg cttcgtcatc atggcatcgt
     2701 catggcactc gagctcgccc ctttccgcgt gtcgtgtcgt ggccgaaggc cgaacgcatc
     2761 gggcaagggc cgtgccaggt catgtggacc gtcacgatct cgcaacccgc gctcgcgacg
     2821 tctcgggcgg gcggccatac cgacctgcga cgcatccccg cgcgtcgggg gtccgtcgcc
     2881 gtcggtccgc ccatcatcgc aggatgcgcg cggtgtccga tagattgaac ttggtcatgt
     2941 cggagatcgc ctccgcgcgc gcgaggatcg aatcgcggtc ggctttggcg agccccgaga
     3001 cgccgaagcc ctcgacagtg aacgacgcga ggacgcagcc gtacacgacc gcgcgcctca
     3061 gattgtggtc gttcacccgg gccatgcgcg cgagggcgcc gacgaatccg ccggcgaagc
     3121 tgtcaccggc gccggtcgga tcgatgacgt tgtcgatcgg ataggccgga caggtgaaga
     3181 agacctcggg gccctggaga aacgcgccgt gctcgccctg cttgatgacc accgtttccg
     3241 ggccgagcga cagaatggcg cgcgccgcct tgtgcgtcag tgcttcgccg gtcagctcgc
     3301 gggcctcctc ctcgttgatg atcacgatcc gcaccttctt cagcgtctcg aggagctcgt
     3361 ccttcttggt ccggatccag tagttcatcg tgtcggcgag aaccagcctc ggctgatcga
     3421 gctgctccaa gacctcgcgc tggagcacgg gatcgatgtt cgcgagaaag acgttcttga
     3481 gcgagcgggt ccgctccggg agatcgggct tgaagcgctc gaacacgttg agctccgtcc
     3541 ggatcgtctc gcgcgtgttc atgtcgtcgc tgtagacgcc ttcccaccgg aacgtctttc
     3601 cgtcctggat ctcgagcccc gccaggtcca cgtcgggctg gtccaggagg gcgcgcgcct
     3661 cgcgggggaa gtccttcccc acgacggcca ccagggagac cggcatgaag tgccgcgcgg
     3721 cgagcgagaa gtacgcggcc gaaccgccca gcacctcggt cacccgcccc gcgggtgtct
     3781 ggatcgtgtc gagtccgatc gagccgacga cggcgatgct catgcgctac atggcctccg
     3841 gcgcgctcac gccgagcagg ccgagcccgc gccggaacac cgctccggtc gccttcgtaa
     3901 gaaggaggcg agcggcggtg agcggcgcgt cggacgtcac gacgcgatgc tggtggtaga
     3961 aggaatggaa cttcgccgcc aaatccttga gaaacgcggg aatgcggtgc ggctcgcggc
     4021 tccgggccga ggccaggacc agggacggga acgcggcgag tccgcggagc agcgtgagcg
     4081 tctccggctc ggcgaggagc gcgagatcgg cccgctcggg atcgggctcg ggcacgccct
     4141 gcgcccgcgc gtagtcgagc acgtgcgcga ttcgcgcgtg cgcgtactgc acgtagtaga
     4201 ccggattgtc ctcggaccgc ttcacggcca gctcgaggtc gaagtccatg gggctttctg
     4261 cacggcgcat gaggaagaag aagcgcgcag cgtccacgcc cacctcctcc accagctcct
     4321 cgagcgtgat gaactccccg ccgcgcttcg acatcttcac cgtctcgccg ccccggagga
     4381 ggcgcaccca ctgcgccacg atcacctcga accggtcctt ctcccagccg agggcgtcga
     4441 ggaccccctg catccgctgc acgtggccgt ggtgatccgg acccagaatg tcgatgagcc
     4501 ggtcgaagcc gcgcgtgaac ttgtcgtggt gataggccgt gtcggcgagg aagtacgtcg
     4561 gctcgccgtt cgaccgcacg atgacccggt cctcctggtc gccgaactcg gtcgagcgga
     4621 accagaccgc tccctcccgc tcgtcgagca cgccccgctt ctcgagcatg gcccgcgcgt
     4681 cctggaccgc gccccccgcg tgcaggctgg actccaagaa ataccggtcc atccgcgcgc
     4741 cgaagcgctc caggtcgtgg cgttggatct cgaggttctt gtcgatgccc cagcgaccga
     4801 agcgggcgcg ctgctccctt tcggggagcg cgagccacgc gcgtccctcg gcctcgtcga
     4861 gcgccgcggc gaggtcggcg acgtacgctc cctgatagcc ctcttccggg atcgcatccg
     4921 cgggaacgcc gatccgctcc tggaagcgcg tgcgcaccga ggcgccgaag agctcggcct
     4981 ggagcccggc gtcgttcacg tagaactcgc cttcggcgcg atgccccgtc gcgttgaaaa
     5041 gccggagcag cgtgtcgccg aggctcgcgg cgcgtgcgct cacgatgttg agggggccgg
     5101 tcggattggc gctcacgtac tcgaccagga tccgctcgcc cttccctgcg tcgctcccgc
     5161 cgtagccgtc gggatccccg agcacgcggc ggaccgtttc ctggagccac gcgggcgcga
     5221 ggcggaagtt gatgaagccg gcgcccgcga tctcgacggc ggcgatcacg gccgcgtcca
     5281 tctcgagcgc gtcgacgagc gccttcgcca cctcgcgcgg cgggcgcttc gcggtcttgg
     5341 ccagggtgag cgcgacgttg gtggaccagt cgccgtgatc cggatcgcgc gggacctcga
     5401 tttcgacgcg cgcgccgccc ggcggggtca ggccggtctt ggagaccgcg tcctcgaccc
     5461 gatcgacgag ataggcctca atcggccacg tcttcacgag cagcatcgcc ccacagcttg
     5521 tcgagaaggt agtactcgcg tgcacgcgcg tggaacacat ggacgaccac gtcgacatag
     5581 tcgaggagga tccagcg
//
LOCUS       DASTKC010000050         6011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_37706, whole genome shotgun sequence.
ACCESSION   DASTKC010000050 DASTKC010000000
VERSION     DASTKC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37706"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFR25_02130"
     CDS             complement(<1..202)
                     /locus_tag="VFR25_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939878.1"
                     /translation="MISALMLAVIPGVLLPWILIPLRSQLLFGLLPVIPLALVYARWI
                     ASRRAGLALAGAGLWALGLSLST"
     gene            251..1801
                     /locus_tag="VFR25_02135"
     CDS             251..1801
                     /locus_tag="VFR25_02135"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET9939879.1"
                     /translation="MLQEYTSTALLDYALPAHENGMRAALESVRARLGKSYPLVIDGK
                     RITTPDHFQSANPANPSECLGNFSKATVEHVNLAVEAAAKGFASWSAIPAEERANVIL
                     RAAGILRRRRHEMSAIMVLEVGKNWGEADGDTAEAIDFLEFYAREMLRYSDRQPITPN
                     PGESNVFEYLPLGVGAVIPPWNFPCAIAMGMTAAALVTGNTAILKPSSDAPLTAWTIF
                     EIFEEAGVPPGALHFLSGPGGAMGDALVEHPKVRFVAFTGSMPVGIGINEKAARVQKG
                     QIWLKRAILEMGGKDFTIVDEGADLEAAVNGVYAGAFGFQGQKCSACSRAIVHQSLVG
                     EFEERLKDKVAGMTIGSTEKRENYLGPVVSARAEKTILEYMEVGKGEGRLLSGGGKHS
                     GPGFFLQPTVIGGVKANARIAQEEIFGPVLAIIPASSFEEEIAIANGTIYGLTGAYYS
                     RDRAKIVEAKRRLFCGNLYINRKCTGALVGIHPFGGFNMSGTDSKAGGRDYLGLFMQG
                     KVTTELVG"
     gene            2039..2923
                     /locus_tag="VFR25_02140"
     CDS             2039..2923
                     /locus_tag="VFR25_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939880.1"
                     /translation="MPRWIRFILVVCLPLFAFSTAHAAVISLTLVGGAMGMDGSTVLA
                     DPLAQESLTGTTLSQIGVPAIWDLTAPFPMDLTFRLTNISDVDLLFPDALPGVSVLTE
                     VYGLAGYTVRQNIAAGGARGSNGAAGTDGAVSRTALDMTETLDAIMGAASGSNGGGGG
                     AATGVANNFISITGASGAELAAYLANLTLHPGEHVDIAGFAHVLAFGRADEAAKIGFG
                     FDLPTFSFGGTSFTTGAWTATCGGPRPDVVEEPPVTTTSTTTSSAPLPGTSGLMLIGG
                     LAGALGRRRDFGRLLNRA"
     gene            complement(2867..4318)
                     /locus_tag="VFR25_02145"
     CDS             complement(2867..4318)
                     /locus_tag="VFR25_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET9939881.1"
                     /translation="MPRETAAVMSAVERRYHFSGVGGSGMAPLAILTASRGALVTGSD
                     RNHDRGIALPVFDALRAAGVRLVPQDGSGITPDLTAFVHSTAVETGNPDFLRAVELDV
                     PRIRRGSFLAQVASEHRTIAVAGTSGKSSVTTMIAHILVEAGLDPSFLGGGAAVSLPG
                     AIPPGSIRVGGSNWLVIETDESDGSVAEFAPAISVLTNLSRDHKEIEEVARMFEALLT
                     ATTECAVMHSGDPILGRIRRPESLPHTYVALEGRQAWIPPDMIARSIVLEPDSVRFEI
                     DDVAVSVPFPGILTVENTLLAIAAATCAGVPLADAAHAMGTFAGVRRRLERIGAARGV
                     DVYDDFGHNPVKIQAALEALRPRGAFWVYYQPHGYGPTRFFRKELVETFRASFRPGDH
                     LLLAPIYDAGGTADRSIRSEHLVEELRGLGVNATLAETRGEAARAIALGSRPGDRAVV
                     MGARDDTLPAFARDVLRAIQQPAEVAPPAQRSR"
     gene            4446..4521
                     /locus_tag="VFR25_02150"
     tRNA            4446..4521
                     /locus_tag="VFR25_02150"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4479..4481,aa:Ala,seq:ggc)
     gene            4645..5316
                     /locus_tag="VFR25_02155"
     CDS             4645..5316
                     /locus_tag="VFR25_02155"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9939882.1"
                     /translation="MIPRNLKAMLLLVLGAMSLISDGCAHSGSRVGGEGTSKTGTLRG
                     KVMDENLRGIENVRVAISGTSEEQLTHADGSFLFARVPSGSYSVSARATGYVSTSRGG
                     VLVDVGRVTFVELALKEVAVRMMRSQEVPADQAGVISGTVVDERDAPLMYAIAFDMNS
                     TRAASTDAGGHFLLSNLPAGAYAIKVKLIGFATEIREGVQVVGRDTTKVVFKLKEQPV
                     QMRIR"
     gene            complement(5320..5817)
                     /locus_tag="VFR25_02160"
     CDS             complement(5320..5817)
                     /locus_tag="VFR25_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939883.1"
                     /translation="MNAVRTAPGALPRAQRALLLAAAAAAGLALFMPGAAHGAVDPTA
                     QVPEFNRICSDLVHAREDLRMGLLEEPAFGNRILDLFVQADSLRTALLATAPGLKLPG
                     TPAFAMERGLRYLIESLRWNYVGVDRANGFNFVDADRALQAAVAWRSGAMGGGAVSMG
                     VEAGP"
BASE COUNT         1018 a         1956 c         2069 g          968 t
ORIGIN      
        1 ccgtggagag ggacaggccc agggcccaga gccccgcgcc ggcgagcgcg aggccggcgc
       61 ggcgcgacgc gatccagcgc gcgtagacca aagcgagcgg gatcacgggg aggaggccga
      121 acaggagctg gctccggagc ggaatgagga tccacgggag caggaccccc gggatgacag
      181 ccagcatcag agcggatatc atgagggccg cagtatcctc gaatccaccg acccccgaca
      241 aggagaagtg atgcttcagg agtacacgag caccgccctg ctcgattacg cactgcccgc
      301 tcacgagaac ggcatgcgcg ccgcgctcga gagcgtgcgg gcccgtttgg ggaagtccta
      361 tccgctcgtc atcgacggga agcggatcac gaccccggac cacttccaga gcgcgaaccc
      421 cgccaaccca tcggaatgcc tcggcaactt ctccaaggcc acggtcgagc acgtgaacct
      481 cgccgtcgag gccgcggcca agggcttcgc ctcgtggagc gccatcccgg ccgaggagcg
      541 cgcgaacgtc atcctgcgcg cggcggggat cctgcgccgg cgccgccacg agatgagcgc
      601 gatcatggtg ctcgaggtgg ggaagaactg gggcgaggcg gacggcgaca cggcggaggc
      661 gatcgacttc ctcgagttct acgcgcgcga gatgctccgc tactcggacc gccagcccat
      721 cacgccgaat ccgggcgaga gcaacgtgtt cgaatatctc ccgctcggcg tgggcgcggt
      781 gatcccgccc tggaatttcc cgtgcgcgat cgcgatgggc atgacggcgg ccgcgctcgt
      841 gaccggcaac acggccattc tgaagccgag cagcgacgct ccgctcacgg cctggaccat
      901 cttcgagatc ttcgaggaag cgggcgtccc tcccggagcg cttcacttcc tgagcggtcc
      961 cggcggcgcc atgggcgacg ccttagtcga gcatcccaag gtgcgcttcg tcgccttcac
     1021 cggctcgatg ccggtcggaa tcgggatcaa cgagaaggcg gcgcgcgtgc agaagggcca
     1081 gatctggctc aagcgcgcga tcctcgagat gggaggcaag gacttcacga tcgtcgacga
     1141 aggcgccgac ctcgaagcgg cggtgaacgg cgtctacgcg ggcgcattcg ggttccaggg
     1201 gcagaagtgc tccgcctgct cgcgggcgat cgtgcaccag agtctcgtcg gggagttcga
     1261 ggagcgcctc aaggacaagg tcgcgggcat gaccatcggc tcgaccgaga agcgtgagaa
     1321 ttacctcggc ccggtcgtga gcgcgcgggc ggagaagacg atcctcgagt acatggaggt
     1381 cgggaagggc gagggaaggc tcctctccgg aggcggcaag cactcgggtc caggcttctt
     1441 cctccagccg accgtgatcg gaggcgtgaa ggcgaacgcg cggatcgcgc aggaggaaat
     1501 cttcggcccc gtgctcgcga tcattccggc ctcgtcgttc gaggaggaga tcgcgatcgc
     1561 caacgggacc atctacggtc tgaccggtgc gtactactcc agggaccgcg cgaagatcgt
     1621 ggaagccaag cggcggctct tctgcgggaa cctctacatc aaccggaagt gcacgggcgc
     1681 tctcgtcggc atccatccct tcggcggctt caacatgagc gggacggact cgaaggcggg
     1741 cggacgcgac tacctcgggc tcttcatgca gggcaaggtg accacggagc tggtgggtta
     1801 ggctccgcgt aggtgtagca ccgtcgaccg gcgttggcga tcctgccaac gccggtttgc
     1861 gctttggggc cacgaggtcg gtcgcaggcc gtccaccgcg gtcgctgcgc tcctcgatcg
     1921 gcagcatgcc ctcgcgattc tccctcggtc cagtcgtcgg gttctgcctt tcggcacatc
     1981 cttcgcattg ccccgctcgc ccccagcctc gagtcccatt ccatggaggt gagccgccgt
     2041 gccacgctgg atccgcttca tcctggtcgt ctgtctcccg ctgttcgcgt tctccacggc
     2101 tcacgccgca gtcatttcac tgaccctggt gggtggtgcg atggggatgg acggctcgac
     2161 cgtcctcgcc gatccgctcg cgcaggaatc gctcacgggc accacgttgt cccagatcgg
     2221 cgttccggcg atctgggacc tcacggctcc gtttccgatg gacctgacgt tccgcctcac
     2281 gaacatctct gacgtcgatc tcctctttcc cgacgcgctc cccggcgtgt cggtgctgac
     2341 cgaggtctat ggactcgccg gatacacggt caggcagaac atcgcggctg gaggcgcccg
     2401 aggatcgaac ggcgcggccg ggacggacgg cgccgtgagc cggaccgcgc tcgacatgac
     2461 cgagacgctg gacgcgatca tgggcgccgc gtcgggctcg aacggcggtg gaggaggagc
     2521 cgcgacgggg gtcgccaaca acttcatttc gatcaccggc gcgtccggcg cggagctggc
     2581 ggcctacctc gccaacctca cgctccatcc aggcgagcac gtggacatcg cgggattcgc
     2641 gcacgtcctc gcgtttggtc gcgcggatga ggcggccaag atcggattcg ggttcgatct
     2701 tccgaccttc agcttcggcg ggaccagctt cacgaccggc gcgtggacgg caacgtgcgg
     2761 aggaccgagg ccggacgtcg tggaggagcc gccggtgacg accacgtcga ccaccacgag
     2821 ctcagcgccc cttccgggga cgtccggact gatgttgatt ggggggctag cgggagcgct
     2881 gggccggcgg cgcgacttcg gccggctgct gaatcgcgcg tagcacgtcg cgcgcgaagg
     2941 cggggagcgt gtcgtcgcgc gctcccatga cgaccgcgcg gtccccgggg cgcgagccca
     3001 gggcgatcgc gcgggccgcc tcgccgcgtg tctcggcgag cgtcgcgttc acaccgaggc
     3061 cacggagctc ctcgacgagg tgctccgacc ggatggagcg gtctgccgtt ccgcccgcgt
     3121 cgtagatcgg cgcgaggagg agatggtcgc cggggcggaa cgaggcacgg aaggtctcga
     3181 ccagctcctt ccggaagaag cgcgtcgggc cgtagccgtg cggctggtag tagacccaga
     3241 aggcgccgcg gggccgcaag gcctcgagcg cagcctggat cttgaccggg ttgtgaccga
     3301 agtcgtcgta gacatcgact ccgcgcgcgg cgccgatccg ctcgaggcgc cggcggacgc
     3361 ccgcgaaggt tcccatggcg tgggccgcgt ccgcgagagg gactccggcg cacgtggccg
     3421 cggcgatcgc gagaagcgtg ttctccaccg tgaggatccc tgggaaggga acgctcaccg
     3481 cgacgtcatc gatctcgaag cgaaccgaat ccggctcgag cacgatcgag cgcgcgatca
     3541 tgtccggtgg aatccacgcc tgccgccctt cgagggcgac ataggtgtgg gggagggatt
     3601 caggacgccg gatacggccc aggatcgggt cgcccgaatg catgaccgcg cactcggtgg
     3661 tcgcggtgag gagcgcctcg aacatgcgcg cgacctcctc gatctccttg tggtcgcggc
     3721 tcaggttcgt gagcacggag atggccggcg cgaactcggc cacgctccca tcgctctcgt
     3781 cggtctcgat cacgagccag ttgctcccgc cgacgcggat gctacccggc gggatggcgc
     3841 cgggcagcga cactgcggcg ccgccgccga ggaaggaggg atcgagccca gcctcgacga
     3901 ggatgtgcgc gatcatcgtc gtcacggacg acttgccgct cgtcccggcg acggcgatgg
     3961 ttcggtgctc cgacgcgacc tgtgcgagga acgagccgcg gcggatgcga gggacatcga
     4021 gctccaccgc gcggaggaag tcgggattgc ccgtttcgac cgcggtggag tgcacgaagg
     4081 cggtgaggtc gggcgtgatg ccggatccgt cctgaggaac gaggcgcacg cccgcggcgc
     4141 gaagcgcgtc gaacaccggc agggcgatcc cgcggtcgtg gttccggtcg gagccggtca
     4201 cgagggcgcc ccgtgaggcc gtgaggatcg caagaggggc cattccgctc cctcccacgc
     4261 cgctgaagtg gtaccgccgc tcgaccgcgc tcatcacggc cgcagtctcg cggggcatcg
     4321 gcctagggtc aaggaaagtc gcggacggac gcgccggaag gcaccgggcg ccgcgggcca
     4381 cccgagggcg ctcgggttga cgctcggact catcccgggt agattgacgc ctgtcggcac
     4441 gggatggggc tgtagctcag atgggagagc gcttgactgg cagtcaagag gtagcgggtt
     4501 cgatccccgc cagctccacc aaccctgcct caccacgttc ttaggcttca ccaacgagtc
     4561 ctcccctcga cgaagccctt cggcaccccc gcgacacacc ttgaggtccc ccgaggttca
     4621 cggctagccg gtttcgaggt ttccatgatt cccagaaacc tgaaggcgat gttgctgctc
     4681 gtcctcggag caatgtccct tatctcggat ggatgcgccc attccggatc ccgggtcggc
     4741 ggcgagggaa cttcgaagac cggcaccctc aggggcaagg tgatggacga gaatctcagg
     4801 gggattgaaa acgtacgcgt cgcaatatcg ggtacctccg aagagcagct gactcatgcc
     4861 gacggcagct ttctcttcgc cagggtccct tcaggttcat attccgtctc ggcaagagcg
     4921 accgggtacg tctccacctc ccgtggcggg gttctagtcg atgtgggtcg cgtgaccttt
     4981 gtggagcttg ccttgaaaga ggtagcggtg cgaatgatgc ggtctcagga agtccctgct
     5041 gatcaagccg gcgtaatctc gggaactgtg gtcgatgagc gggacgcccc tcttatgtac
     5101 gccatcgcct ttgacatgaa cagcacccga gctgcgtcaa cagacgctgg cggtcacttc
     5161 cttctttcaa atctgcccgc tggagcttac gcgatcaaag taaagcttat tggctttgcg
     5221 actgagattc gtgagggagt gcaggtcgtg ggtcgtgaca ctacgaaggt ggttttcaag
     5281 ctgaaggaac agccggttca gatgaggatt cgttagcagc tacggtcccg cctcaacccc
     5341 catactcacg gcaccgccac ccattgcccc gctccgccac gccacggccg cctgcagtgc
     5401 gcgatccgca tccacgaaat tgaacccgtt cgccctgtcc acccccacgt aattccagcg
     5461 cagcgactcg atcaaataac gtaagccgcg ctccatcgcg aaggcgggcg tccccggaag
     5521 cttgagcccg ggggcggtcg cgaggagcgc ggtgcgcagg ctgtcggcct gcacgaagag
     5581 gtcgaggatg cggtttccga acgcgggctc ctcgagcagg cccatacgga ggtcctcacg
     5641 ggcgtgcacg aggtcggaac agattcggtt gaattcgggt acttgggcgg tagggtccac
     5701 cgcgccgtgc gccgcgcccg gcatgaagag ggcgaggccg gcggccgctg ccgcggcaag
     5761 gagaagcgcg cgttgggctc ggggcagggc cccgggcgcg gttcggactg cgttcatgtt
     5821 ggccttcctt agcgaggccc cccggcctca cacataaaat ttcggtaagg aggcgcgtat
     5881 ccttgactag aaatcgcccg gttttttgca tcggcctctc gatttgcccg gagcccggcc
     5941 cttcgattga cgggggaccg ccgctctgat agcctgcagg cccatgctcc acaggtcgaa
     6001 tcccattcat t
//
LOCUS       DASTKC010000051        17971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_38575, whole genome shotgun sequence.
ACCESSION   DASTKC010000051 DASTKC010000000
VERSION     DASTKC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17971
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38575"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..439)
                     /locus_tag="VFR25_02165"
     CDS             complement(56..439)
                     /locus_tag="VFR25_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939884.1"
                     /translation="MIVAAQLLLTLGVLAFILTPLILNRGERSWAEPELEAQRRSIAE
                     RKGRLYGQLLDLDFDRDSGKISPEDHGRLREEIMAEVVQVLAEEEKTVPQGQRRPVVL
                     EGGDRVERMIEEYKRARRPGAGAAR"
     gene            complement(436..954)
                     /locus_tag="VFR25_02170"
     CDS             complement(436..954)
                     /locus_tag="VFR25_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET9939885.1"
                     /translation="MIRPSILATLALVAGLLTAFAVPAHATESAAGRKDPARAITSQL
                     ICPCSCGEILSGCTCETGKSMQGFVTDELKKGRTKDEVTASLVSKYGEVILGAPKAQG
                     FNLLVWVLPFVATAIGFVIAALVLKRWVGRPQPAGAGGLALPGQAPRTTAEQDLADLR
                     ARAEAELRGLGE"
     gene            complement(951..2912)
                     /locus_tag="VFR25_02175"
     CDS             complement(951..2912)
                     /locus_tag="VFR25_02175"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET9939886.1"
                     /translation="MSEIGKLSLFLAFLCSCYAVGAFVVGLKSGMAGPLRSGRRAVWA
                     VCGLTLLATILLVRALVARDFSYRYVAEHTSLDLPTMYAFSSLWAGQEGSLLLWLLIL
                     SAYGAAFLWCYRKRLDPFYDAVAMVVASVMIFFTSLLSFVSSPFRILATPPPDGNGLN
                     PLLQDPGMMIHPPILYTGYVGFIIPFAFGIAVLIMNRTGTRWIEEVRRWTLFNWMFLG
                     FGILLGARWAYIELGWGGYWGWDPVENASLMPWLVGTAFLHSVMIEQRRGMLKTWNIV
                     LIVLTFELSIFGTFLTRSGVLTSVHSFAESDIGPWFMGFILASGTIATALILYRKALL
                     ESENRMDAVVSREGSFLFNNVLFVALCFATFLGTTFPVLSEAVTGSKISVSAPFFNRV
                     NVPIALALLMLTGIGPVLSWKRATGAFVKRNFMIPAFVGVLATLVCIPFGLRGVYPIV
                     CVFGAAFVIAAVVMEFVRGVQARRDVDAPANPAAILHLVQKNKRRYGGYIVHLGVVMI
                     FVGVLGSSVFQKEAHSPLQPGQSMAIGPYALTLDRVEDTQIQNATQTRAHVQVTRNGK
                     RLADMNPSKAMYTKSQQPMTEVALRSTAAEDLYLILGGVNEDGSASIQAYINPLVAFV
                     WAGGVVMVIGTIICLADRMRLKREEKLLA"
     gene            complement(2909..3322)
                     /locus_tag="VFR25_02180"
     CDS             complement(2909..3322)
                     /locus_tag="VFR25_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET9939887.1"
                     /translation="MNRTTLRLILVTVAVLGCVAWLISSGVKATGMQYMTVTELAQLT
                     APPRQGGFRLDGKVQAGSIAYDQKAPRLHFQMTDGKQAIGVVYEGLMPDAFADGREVV
                     VEGSYHHDDRTLHATKLVTKCPSKYEAEGLGKDRS"
     gene            complement(3444..4124)
                     /locus_tag="VFR25_02185"
     CDS             complement(3444..4124)
                     /locus_tag="VFR25_02185"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9939888.1"
                     /translation="MIPLRDKNPSGTFPLVTLAIIAGCTVTFLYELAAPSLQQLFASY
                     ALIPARVTLGLESGTGPLGSLGAIVPFLTSMFLHGGWLHLIGNMWYLWIFGDNVEDTL
                     GPVRYLLFYFLCGIAAGATHVLVSPGSTAPTVGASGAIAGVLAGYASLFPRARVVTLI
                     PIVFYFSIVELPAVALLVVWFLIQGLSGFLSLGAPGSTGVAWGAHVGGFLAGLILIRI
                     LRPRKVMA"
     gene            complement(4137..4919)
                     /locus_tag="VFR25_02190"
     CDS             complement(4137..4919)
                     /locus_tag="VFR25_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9939889.1"
                     /translation="MRSRARTAIFALLLLLTDAIPASAAPLAVATPAMVAPASYVPAA
                     PAPLAALSWRKSAAFAAAAASDAPDSIPSEVTLPGGSTVLRETVMAGAAIGVGFLLDD
                     TIEPGSGEDTFLDNAGQALGSPVVLVGGTVLMALYGWKAHAPKELNTAKRLALAITAT
                     SLTVLTLKTTTNRERPDESDDLSFPSGHTANTMAVATVLDREYDGVVPWIAYGAAAFV
                     GASRVAGNHHWFSDVMAGAVIGRFFGRLVTHNDVQHHNVSHH"
     gene            complement(4937..5554)
                     /locus_tag="VFR25_02195"
     CDS             complement(4937..5554)
                     /locus_tag="VFR25_02195"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9939890.1"
                     /translation="MQRALPAALLLPALVLASILAPAPSRASTTTATLLRKSAIAAGL
                     IGAAFALDGAVKRDGIDEGPGEVIEEPGEVVGAGQFVFGGTAVLGAIGLVTGDREAMH
                     TAKDLAVALTATSAAVWALKYTTHRERPDGSNHYSFPSGHTAVAFAAASVIDKRYGGI
                     AGWGAYTAAALAGEARIADNHHYLSDVVAGAVLGTVIGHLVAHAP"
     gene            5684..6757
                     /locus_tag="VFR25_02200"
     CDS             5684..6757
                     /locus_tag="VFR25_02200"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9939891.1"
                     /translation="MPAKRFRAVDERVKAAIAEGLIPGAVAIVGRSQGPVHRVVAGHR
                     AVRPRREPMTAATIFDLASLTKVMATAPLAVEEATHGRLRLADPLERHLVETRDTQVG
                     TIPLHRLLTHTAGFVPDNPIEDYRGSRAALVRAIAEEPLESEPGTKFTYSDVGYILLQ
                     IVLERRARKRLDVLAQEALFRPLGLKDTRFGTRVKDRPHTAPTELVRGRWLRGVVHDP
                     RARSRALLGIGGHAGVFGTAADTARFCEMILRRGAYGRRRVLAEATVRAMTTDQCEPG
                     VGARRGFGFDIQSPYSAPRGLRFSQRSFGHSGWTGGSLWIDPEADAYVVLLTNAIHPD
                     GHRDLKALRSDVATAAAEGLLKI"
     gene            complement(6743..9238)
                     /locus_tag="VFR25_02205"
     CDS             complement(6743..9238)
                     /locus_tag="VFR25_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9939892.1"
                     /translation="MLALAIAIPLLAIMQVQVRSTHRSVEENEALRGAALARLGAMAV
                     DEYLRGIADYAEAYAEHPEILQGANRRDERAVRAVLARLIRGNPRVDRTFVTDVTGVE
                     WSDYPRDPSVIGKNFAFRDWFRGVSSGETTYVSSSYHRMAGRQERSIAIATPIRSGTG
                     PVSGYLVAQVLVSSLERQLAQPVRPDSGGIVVIDRDRAVVTELGFPPGSAPGVELGAL
                     VRAKDGGGSLGTIQVGGAPYLVAASPSPTLGGRVLALRSLRAALAPARAMELVVFLLV
                     LAVALLLALFVTAMIEQRSRRIAATERERAEATLRESERRFRATFEQAAVGIAHLSLA
                     GAFVRVNQRLCDILGYSRSELLHMRVKEFMRPEDLAGGAGCPPEEVLQHIGEWKEGIC
                     RVDQELKRKDGTMVWCHLTLAPVSDGSGTPEYFTAVVEDITERKRLEEQFLQAQKMRA
                     IGQLAGGVAHDFNNLLTTILGYCELIQRKLPVNDALRGYVDEIAMAGQRAAALTSQLL
                     AFGRKQILRPLPVNLNSVIEDMDRLLRRLVGDEVQLETRLDPKLGTVRADQGQMEQVL
                     MNLVLNARDALPHGGRVTLETRNEWIESGSAPERTGLAPGAYAVVSVKDTGVGMDSAV
                     RAQLFEPFFTTKEKGKGTGLGLSTVYGIVKQSGGGITVQSEPGQGSRFEVYLPTAKAT
                     DVHAPRVSEPHPEEGAPAGGSETILLVEDDATLRALTHRVLEERGYRVLEAPSGIDAL
                     AMVERIREPIHLLLTDVVMPRMSGAALAEGVRDRHPATRVVFMSGYTDEAVVRQAAAG
                     GGIRFIQKPYKPEGLLRTIRAALDAPDPVSNLK"
     gene            complement(9337..9915)
                     /locus_tag="VFR25_02210"
     CDS             complement(9337..9915)
                     /locus_tag="VFR25_02210"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET9939893.1"
                     /translation="MKEMTDLAYEIERLEQEIAERSERLAEHKRLLPTTPVSDYTLTG
                     PHGPVKLSQLFGQGKDLIVIHNMGKGCRYCTLWADGWNGILPHLENRAAFVVITPDSP
                     DTQAQLAHSRGWNFRMISSHGSMFTEDMGFRSEKSWLPGVSTFYKNDDGSIVRVARAG
                     FGPFDSFCSAWHLFGLLKDGVNGWEPKYRYGT"
     gene            10058..11083
                     /locus_tag="VFR25_02215"
     CDS             10058..11083
                     /locus_tag="VFR25_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939894.1"
                     /translation="MNDVRPGRPTQPPRRAATIAGLAALALVSMGAAFESHWQDGKAE
                     LDGYRYTVTRYGHARAGTAVMVYVTEPFSEAKRVKVDHPTPGSADVVEALKLNFIRDF
                     QTGIYDYNTMVSLFARSRDFSPMKISFSGSEWCGNVYEEMLFERYHLADRYQSYFEGE
                     SDARRLTLVKPGIAEEELFILLRDLRSDWLAPGQKRKIAFLPAAFYRRLTHRPLIWTQ
                     AVIERGKGVEAVTVPAGRFDAVRYVVKVADGREGTFWVERAYPHRILRWTWLPAAGMT
                     QRGGWSPAEGLDQGELAGSQRLPYWQLHEPGDEKYLAQLGLKPQTAIVGGTTRLRGDV
                     GTTPSKR"
     gene            complement(11170..12471)
                     /gene="aceA"
                     /locus_tag="VFR25_02220"
     CDS             complement(11170..12471)
                     /gene="aceA"
                     /locus_tag="VFR25_02220"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET9939895.1"
                     /translation="MPLPASRWAGVVRPYRNEDVERLRGSVKVEHTLAQLGARRLWHL
                     LTTREYVNALGALSGNQAVQQVKAGLEAIYLSGWQVAADANLAGQTYPDQSLYPANSV
                     PSVVRRINQALLRADQIEHAEARKAPSQNGHSQNGHGANGHNAPAARTWLAPIVADAE
                     AGFGGPLNAFELMKSMIEAGAAAVHFEDQLSSEKKCGHLGGKVLVPTGHFIRTLVAAR
                     LAADVLDVPTLIVARTDADSAKLITSDVDERDREFLTGNRTSEGFFGYKGGIECAIAR
                     AIAYAPYADLIWMETSTPDLEEARQFAEAVHAKFPGKMLAYNCSPSFNWRAKLDPETI
                     AKFQKELGAMGYKFQFVTLAGFHSLNHGMFELARAYKDRGMAGYAELQTAEFDSEKHG
                     YSATRHQREVGTGYFDEVMQVISNGSCSTLALDGSTEKAQF"
     gene            complement(12624..14216)
                     /gene="aceB"
                     /locus_tag="VFR25_02225"
     CDS             complement(12624..14216)
                     /gene="aceB"
                     /locus_tag="VFR25_02225"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HET9939896.1"
                     /translation="MNLSALTSTGASVEAPVRPGYERLLTPEALAFLESLTRAFRPTL
                     EQLLGDRASRLSIAAPLEFLDETLPVRQGDWTIAPVPGDLTRRIVEITGPVDRKMVIN
                     ALNSGADCFMADFEDSNAPTWSNNVEGQLNLMDAVRRTIDYVDPASGKEYRLADRTAT
                     LIVRPRGLHLPERHLHVDGRPVPGALFDFGLYFFHNAHELIRRGTGPYFYLPKLESYR
                     EARLWNEVFLAAEETLDVPRGTIKATVLIETLPAAFQMDEILYELREHVAGLNCGRWD
                     YIFSFIKQRRNDPAAVLPDRSQVTMTQPFLRAYTQLCIRTCHRRGAHAMGGMAAQIPI
                     KSDPEANHAALEKVRQDKLREVTDGHDGTWVAHPGLVALAREVFEAVMDGPNQLDRKR
                     EDVEVTGSDLLAVPRGTRTEEGLRLNARVGIQYLEAWLEGHGCVPLYNLMEDAATAEI
                     SRAQVWQWVHHKARLEDGAVVDAAHVRAVVEDEMRHVEQEIGSERFRNGRFTEACDLF
                     LELSTAETLEEFLTIPAYSRID"
     gene            14350..15789
                     /locus_tag="VFR25_02230"
     CDS             14350..15789
                     /locus_tag="VFR25_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HET9939897.1"
                     /translation="MKAPALGRKIRALRQAEGLTQAAMAARLGISTSYLNLIENDRRP
                     LGAGLLLTLARAFNIDLRTFAIGEDARLVSDLAEAFGDPLFGERRLDERELREFVSSS
                     PAIARAVVQLHHAYTDARASAESIAAEVVDRQDLSGVDRAGLAAEEVSDLVQRHVNHF
                     PELEAEADRIWKDAKLEDQDFFEGMAHYMERRHSVRVRVLRLGEMGDAVRRFDPERRE
                     LHLSEVLRRGSRNFQLAHQAALLTCPDTLDRIAQDEQLTSPESRALCRVALANYFAGA
                     LLMPYDAFFRAAEEERYDTDLLEHRFRANFEQICHRLTTLNRSGARGIPFHMVRVDIA
                     GNISKKFSATGLRFPRFGGLCPLWNVHAAFLQPNVIRAQLDRFPDGTLYFSFARTIRK
                     HRGGYHTPEVLYSVGLGCDVESAKRLVYADGFDLTNPQAAAPVGITCRLCERLDCRAR
                     AFPSIYQPLKVDENLRGVSFFARPGGTGT"
     gene            complement(16377..16505)
                     /locus_tag="VFR25_02235"
     CDS             complement(16377..16505)
                     /locus_tag="VFR25_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939898.1"
                     /translation="MRHFSVRGFGIRFALALVLPITGIGLVLWTVAQLIVYQLAHA"
     gene            complement(16637..>17971)
                     /locus_tag="VFR25_02240"
     CDS             complement(16637..>17971)
                     /locus_tag="VFR25_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9939899.1"
                     /translation="VAFAAPAALAASAAPAAPAAPAASAVPAASAVPAVPRVFALRFA
                     RIVTGAGPVIEDAVVIVEGDKIRAIGPAARTSLPAGAQEIDLTRYTGIPGLIDVHTHL
                     TYSWDGTPGTNPWQQLSARPTAATVYLAQGNAKHTLETGVTTVRDLGSWDYMDIAMRD
                     LIASGAMMGPRMFVCGYGLHPTLSPNRPGYMEPPGGNADGAAEVMRVVRQNIAAGADV
                     IKVYGSTGSADDVTGDETYTYEELKAAVDAAKGRGKRVAVHSYGPDGARDAVRAGATS
                     IEHATDMDDATLREMAKRGTFYVPTIDHNRYYAENASIFGYDSSAVARLNEYRGRNVE
                     TLRRAVRAKVKVAMGSDALFTMCGENTRELEWFVKAGMTPAQALAAATVNGAALLGQE
                     KQLGALAPGYMADIVAVEGNPLTDIQAVTARVRWVMKAGQVVVDRRLGTAER"
BASE COUNT         2913 a         6045 c         6222 g         2791 t
ORIGIN      
        1 ggcgcggcga tcgcgaggag cgcgaggagc gctcccagcg aggcggcgcg cttcatcacc
       61 gcgcggcccc cgcccccggg cggcgtgcgc gcttgtactc ctcgatcatc cgctccacgc
      121 gatcgcctcc ttcgagcacc actggacggc gctgcccttg cggcacggtc ttctcctctt
      181 ccgccaggac ctgcaccacc tccgccatga tctcctcgcg gagccgcccg tgatcctccg
      241 gagagatctt gcccgaatcc cggtcgaagt ccagatcgag gagctggccg tagagcctcc
      301 ccttccgctc ggcgatggaa cggcgctgag cttcgagctc gggctcggcc cagctccgct
      361 cgccccgatt caggatcaac ggcgtgagga tgaacgcgag aacccccagg gtgaggagaa
      421 gctgtgcggc gacgatcatt cgccgaggcc gcgcagctcg gcttcggcgc gcgctctcaa
      481 gtcggcgaga tcctgctccg ccgtcgtcct gggtgcctgc ccggggagag cgaggccgcc
      541 cgctccggcc ggctggggcc ttcccaccca tcgcttcagc acgagggccg cgatgacgaa
      601 gccgatggcc gtggcgacga acggcaggac ccagacgagc aggttgaatc cctgcgcctt
      661 gggcgcgccg aggatcacct cgccgtactt cgagacgagc gatgccgtga cctcgtcctt
      721 cgtcctcccc tttttcagct cgtcggtgac gaagccttgc atcgacttcc cggtctcgca
      781 cgtgcagccg ctcaggatct cgccgcagga gcagggacag atgagctggc tcgtgatggc
      841 gcgcgccgga tccttccggc cggcggcgga ctccgtggcg tgcgccggaa cggcgaaggc
      901 cgtgaggagg ccggctacga gcgcgagggt ggcgaggatg gagggacgaa tcatgcgagg
      961 agcttttctt cccgcttcaa gcgcatgcgg tcggcgaggc agatgatcgt gccgatcacc
     1021 atcacgacgc cgcccgccca aacgaacgcg acgaggggat tgatgtaggc ctggatcgac
     1081 gcggagccgt cctcgttcac gccgcccagg atgagataga ggtcttcggc ggcggtcgaa
     1141 cggagcgcga cctcggtcat gggctgctgc gatttcgtgt acatcgcctt ggacggattc
     1201 atgtcggcga gccgcttgcc gttccgggtc acctgcacgt gggcgcgcgt ctgcgtggcg
     1261 ttctggatct gcgtgtcctc gacccgatcc agcgtgagcg cgtacgggcc gatggccatc
     1321 gactgccccg gctggagcgg cgagtgcgcc tccttctgga acaccgagga gccgagcacg
     1381 ccgacgaaga tcatcacgac acccaggtgc acgatgtagc cgccgtagcg gcgcttgttc
     1441 ttctgcacca ggtggaggat cgcggccgga ttcgccggcg cgtccacgtc tctacgcgcc
     1501 tgcacgccgc gcacgaactc catcacgacg gccgcgatca cgaaggcggc tccgaacacg
     1561 cagacgatcg ggtagacgcc gcgcagcccg aaggggatgc agacgagcgt ggcgagaacg
     1621 ccgacgaagg ccggaatcat gaagttccgc ttcacgaacg cgcccgtcgc gcgcttccac
     1681 gagagcaccg gcccgatgcc ggtgagcatg aggagcgcca gggcgatcgg cacgttcacg
     1741 cggttgaaga agggggccga gaccgagatc ttggagcccg tcaccgcctc ggagagaacc
     1801 gggaatgtgg tccccaggaa cgtcgcgaag cagagcgcga cgaagagcac gttgttgaag
     1861 aggaacgagc cctcgcgcga gaccaccgcg tccatccggt tctcggattc gaggagcgcc
     1921 ttgcgataga ggatgagcgc cgtcgcgatc gtccccgagg cgaggatgaa gcccatgaac
     1981 catgggccga tgtccgactc cgcgaacgag tgaacggagg tgaggacgcc cgaccgcgtg
     2041 aggaaggtgc cgaagatcga gagctcgaac gtgagcacga tcagcacgat gttccaggtc
     2101 ttcagcatgc cgcggcgctg ctcgatcatg accgagtgga ggaacgccgt tcccacgagc
     2161 cagggcatga gcgacgcgtt ctcgaccgga tcccagcccc agtagccgcc ccagcccagc
     2221 tcgatgtacg cccaccgcgc gccgagcagg atgccgaagc cgaggaacat ccagttgaag
     2281 agcgtccagc gccgcacttc ctcgatccag cgcgtgccgg tgcgattcat gatgaggacc
     2341 gcgatcccga acgcgaacgg gatgatgaag cccacgtaac cggtgtagag gatcggcggg
     2401 tggatcatca tccccggatc ctgaaggagc ggattcaagc cgttcccgtc cggcggcggc
     2461 gtcgcgagaa tccggaacgg cgacgacacg aacgagagga gcgatgtgaa gaagatcatg
     2521 acgctggcca ccaccatcgc gaccgcgtcg tagaagggat cgagccgctt ccggtagcac
     2581 cagaggaacg cagcgccgta cgcgctcagg atgaggagcc agaggaggag cgagccctcc
     2641 tgccccgccc agagcgagct gaacgcgtac atcgtcggga gatcgaggct cgtgtgctcg
     2701 gccacgtagc ggtaggagaa gtcgcgcgcg acgagcgcgc gcacgaggag gatcgtcgcg
     2761 aggagtgtga ggccgcagac ggcccatacc gcgcgccggc cgctccgaag cggcccggcc
     2821 atccccgatt tgagacccac cacgaacgcg ccgaccgcgt agcaggagca gaggaacgcg
     2881 aggaagaggg agagcttccc gatctcgctc acgagcggtc cttgccgaga ccctccgcct
     2941 cgtacttcga cgggcacttg gtcacgagct tcgtggcgtg gagcgtgcgg tcgtcatgat
     3001 gatagctgcc ttccacaacg acctcgcgcc cgtccgcgaa ggcgtccggc atgaggcctt
     3061 cgtagacgac accgatcgcc tgcttgccgt cggtcatctg gaagtggagg cgcggggcct
     3121 tctggtcgta cgcgatgctc ccggcctgga ccttcccgtc gagccggaac ccgccctggc
     3181 gcggaggcgc cgtgagctgg gccagctcgg tcacggtcat gtactgcatg cccgtcgcct
     3241 tgaccccgga cgagatgagc caggcgacgc agccaaggac agccacggtc acgaggatca
     3301 ggcggagggt ggttcgattc atggatcgcc ttgccctggt ttgggttaag tgcgcgggat
     3361 tccggtcatt gtggcagaag gcaggagcga gtcaagcagc gacggtggcc tgctacctcg
     3421 gtagtacccg cgcgcccggc ggattacgcc atcaccttgc ggggacgtaa gattcgaatg
     3481 aggatgagcc ccgccaggaa gcctcccacg tgggcccccc acgccacccc ggtcgaaccc
     3541 ggcgcaccca gcgacaggaa tccgctcaag ccttggatga ggaaccagac gaccaggagc
     3601 gcgaccgcgg gcagctcgac gatcgagaag tagaagacga tcgggatgag cgtgacgaca
     3661 cgggcgcgcg ggaagagcga ggcgtagccc gcgaggaccc ccgcgatggc cccgctcgcg
     3721 ccgacggtgg gcgccgtgga cccgggcgag accagcacgt gcgtggcccc ggcggcgatc
     3781 ccgcacagga agtagaagag gaggtagcgg accggtccga gcgtgtcctc gacgttgtcg
     3841 ccgaagatcc agaggtacca catgttcccg atcaggtgga gccagcctcc atggaggaac
     3901 atcgaggtga ggaacggcac gatggcgccg agcgacccga gcggcccggt cccgctctcg
     3961 agcccgagcg tgacgcgcgc cgggatgagc gcgtaggacg caaagagctg ctggagcgag
     4021 ggcgcggcga gctcgtagag gaacgtgacc gtgcaccccg cgatgatcgc gagcgtgacg
     4081 agcggaaacg tgcccgatgg gttcttgtcg cggagcggga tcatgactct acgattctag
     4141 tggtgcgaga cgttgtggtg ctggacgtcg ttgtgcgtga cgaggcgtcc gaagaaccgc
     4201 ccgatcacgg cgcccgccat cacgtcggag aaccagtggt ggttccccgc gacccggctc
     4261 gctccgacga acgcggccgc gccgtacgcg atccagggga ccacgccgtc gtactcgcga
     4321 tcgagcacgg tcgcgaccgc catcgtgttc gcggtgtgcc ccgaagggaa cgacaggtcg
     4381 tccgactcat cgggacgctc tcgattcgtc gtggtcttga gcgtgagcac ggtgaggctc
     4441 gtcgccgtga tcgcgagcgc cagccgcttc gcggtgttga gctccttggg cgcgtgcgcc
     4501 ttccatccgt agagcgccat gagaacggtg ccccccacga gcacgaccgg gctcccgagc
     4561 gcctgcccgg cgttgtcgag gaatgtgtcc tcgccggacc caggttcgat cgtgtcgtcg
     4621 aggaggaagc cgacgccgat cgcggcgccc gccatcacgg tctcgcggag caccgtcgag
     4681 ccgccgggca gcgtgacctc ggaggggatc gaatcggggg cgtccgaggc tgcggcggcg
     4741 gcgaaggcgg cggacttgcg ccaggagagc gcggcgagcg gggccggcgc ggccgggacg
     4801 taggaggcgg gcgcgaccat cgcaggtgtc gcgacggcta gaggcgccgc ggacgccgga
     4861 atcgcgtccg tcagcaagag gagcagagcg aagatcgcgg tccgggcccg agagcgcatg
     4921 ggccgcggag gatagcctat ggagcgtgcg cgaccaagtg accgatcacg gtcccgagga
     4981 ccgcgcctgc gaccacgtcg gagagatagt ggtggttgtc cgcgatgcgc gcttcgcccg
     5041 cgagcgcggc ggccgtgtac gcgccccacc cggcgatgcc gccatagcgc ttgtcgatca
     5101 cggaggcggc cgcgaacgcc accgcggtgt ggccggacgg gaaggagtag tggttcgagc
     5161 cgtcgggtcg ctcgcggtgg gtcgtgtatt tgagagccca gaccgcggcc gatgtcgccg
     5221 tgagcgcgac ggcgagatcc ttcgcggtgt gcatcgcctc gcgatcgccc gtgacgagcc
     5281 cgatggcgcc gagcaccgcc gtcccgccga aaacgaactg cccggcgccc acgacctctc
     5341 ccggctcctc gatcacctcg ccggggccct cgtcgatgcc atcgcgcttc accgcgccat
     5401 cgagcgcgaa ggcggcgccg atcaagccgg cggcgatcgc ggacttgcgg agtaaggtgg
     5461 cggtggtggt gctggcgcgg gagggggcgg gcgcgaggat cgaggcgagg acgagcgccg
     5521 gcaggaggag tgctgccggc agggcgcgtt gcatgactcc tcctacccca acggaagcgc
     5581 gcgggtccat tgcgagtaga gaacggcaaa gccccccgct tcttgacccc ggaagcccgc
     5641 ggcgcttcgc ccccgcccat cgatgacgta tcctgtcgcg cgcatgccag ccaagcgatt
     5701 ccgcgccgtc gacgagcggg tgaaggccgc gatcgccgag ggactcatcc ccggcgcggt
     5761 cgcgatcgtc ggccggagcc agggcccggt ccatcgcgtc gtggccggcc atcgcgcggt
     5821 ccgcccgcgg cgggagccga tgaccgcggc gacgatcttc gacctcgcgt cgctcaccaa
     5881 ggtgatggcg acggcgccgc tcgcggtcga ggaggcgacg cacggccgac tccggctcgc
     5941 ggacccgctc gagcgccacc tggtcgagac ccgcgacacg caggtcggga cgattccgct
     6001 ccaccggctc ctcacccaca ccgccggctt cgtgcccgac aatcccatcg aggactaccg
     6061 cggctcccgg gcggcgctcg tccgggccat cgcggaggag ccgctcgaaa gcgagccggg
     6121 aacgaagttc acctacagcg atgtcggcta catcctgctc cagatcgtgc tcgaacggcg
     6181 cgcgcgcaag cggctcgacg tcctcgcgca ggaggcgctc ttccgtccgc tcgggctcaa
     6241 ggacacgcga ttcggcactc gcgtgaagga tcggccgcac acggcgccca ccgagctcgt
     6301 gcgtggccgc tggctccgcg gcgtggtgca cgatccgcgg gcgcggtcgc gcgcgctcct
     6361 gggcatcgga gggcacgccg gcgtgttcgg cacggcggcc gacaccgcgc ggttctgcga
     6421 gatgatcctc aggcgcgggg cttatggccg ccgtcgcgtg ctcgcggaag cgaccgtccg
     6481 cgccatgacc accgatcaat gcgagcccgg agtaggagcg cgccgcggat tcggcttcga
     6541 catccagagt ccctactccg cgccgcgcgg actgcgattc tcgcagcggt cgttcgggca
     6601 ctcgggctgg accggcggct cgctctggat cgatcccgag gccgacgcct acgtcgtgct
     6661 cctcacgaac gcgatccatc ccgacggtca tcgggacttg aaagcgctcc gctccgacgt
     6721 ggccaccgcg gccgcggaag gattacttaa gatttgacac ggggtcgggc gcgtcgagcg
     6781 cggcccggat ggtgcggaga agtccctcgg gcttgtacgg cttctggatg aaccgaatgc
     6841 cgcctccggc cgcggcctga cgcacgacgg cctcgtcggt atagcccgac atgaatacga
     6901 cgcgcgtcgc ggggtgccgg tcgcgcacgc cttcggcgag cgccgccccg ctcatgcgcg
     6961 gcatcacgac gtcggtgagg aggaggtgga tcggctcgcg gatccgctcg accatcgcca
     7021 gcgcgtcgat cccgctcggc gcttccagca cgcgataacc ccgctcctcg agcacgcggt
     7081 gcgtgagagc gcggagcgtg gcgtcgtcct cgaccaagag gatcgtctcg ctgccccccg
     7141 cgggcgcgcc ttcctcgggg tgcggctcgc tcacgcgcgg ggcatgcacg tcggtcgctt
     7201 tcgccgtcgg gagataaacc tcgaagcggc tcccctgtcc cggctcgctc tggacggtga
     7261 tgccgccgcc ggattgcttc acgattccgt acacggtcga cagcccgagc ccggtcccct
     7321 tccctttctc cttcgtcgtg aagaatggct cgaagagctg cgctcggacg gccgagtcca
     7381 tgccgacgcc ggtgtccttc acggatacga cggcataagc gcccggcgcg aggcctgtcc
     7441 gctcgggggc ggatcctgac tcgatccatt cattccgcgt ctcgagcgtc acgcggccgc
     7501 cgtgcggcag cgcgtcgcgg gcgttgagca ccaggttcat caagacctgc tccatctgcc
     7561 cctggtcggc gcgcaccgtg ccgagcttcg ggtcgagccg ggtctcgagc tggacctcgt
     7621 cgcccacgag ccgtcgtaga agacggtcca tgtcctcgat gaccgagttc agattcacgg
     7681 gaagcggacg caggatctgc ttccgcccga acgcgaggag ctgcgacgtg agcgccgccg
     7741 cccgctgccc cgccatcgcg atctcgtcca cgtatccgcg gagcgcatcg ttcacgggaa
     7801 gcttgcgctg gatgagctcg cagtagccga ggatcgtggt gaggagattg ttgaagtcgt
     7861 gtgcgacgcc tccggcgagc tggccgatgg cgcgcatctt ctgcgcctgg aggaactgct
     7921 cctcgaggcg cttccgctcg gtgatgtcct cgaccacggc ggtgaagtac tccggggtgc
     7981 cggagccgtc ggagaccggc gcgagcgtga gatggcacca gaccatcgtg ccgtccttcc
     8041 gcttgagctc ctgatccacg cggcagatgc cttccttcca ctcgccgatg tgctggagca
     8101 cttcctccgg cgggcagccc gcaccccccg ccagatcttc gggccgcatg aattccttga
     8161 cccgcatgtg gaggagctcc gagcgcgagt agccgaggat gtcgcagagc cgctggttca
     8221 cgcgcacgaa cgcgcccgcc aggctcagat gggcgatgcc gacggccgcc tgctcgaacg
     8281 tggcgcggaa ccgccgttcg ctctcgcgca acgtcgcttc ggcgcgctcg cgctcggtcg
     8341 cggcgattct cctcgagcgc tgctcgatca tggcggtcac gaagagggcc aggaggagcg
     8401 cgaccgcgag gacgagcagg aagacgacga gctccatggc gcgcgcggga gcgagcgccg
     8461 cacggaggct ccggagcgcc agcacgcggc cgccgagggt gggcgagggg gaggcggcga
     8521 cgaggtacgg cgcgcccccg acctggatcg taccgagcga gcctccgccg tccttcgcgc
     8581 ggaccagcgc gccgagctcg acaccggggg cggagccggg tggaaagccg agctcggtga
     8641 cgacggcgcg gtcgcggtcg atcaccacga tcccgcccga atccggccgc acgggctgcg
     8701 cgagctggcg ctcgagcgag gagacgagga cctgcgccac gagatagccc gagaccgggc
     8761 cggtgcccga gcggatcggc gtcgcgatgg cgatggagcg ctcctgccgt cccgccatgc
     8821 ggtgatacga cgaagagacg taggtggtct caccggacga gacgccgcgg aaccagtctc
     8881 ggaacgcgaa atttttcccg atgacggagg ggtcgcgggg gtaatcgctc cactcgacac
     8941 cggtcacatc ggtgacgaac gtgcgatcga cgcggggatt gccccggatc agccgcgcga
     9001 ggacggcgcg caccgcacgc tcgtccctgc gattcgctcc ctggaggatt tcggggtgct
     9061 cggcgtacgc ttcggcgtag tcggcgatgc cgcgcaggta ttcgtccacg gccatcgcgc
     9121 cgagccgcgc gagcgcggcg ccgcggaggg cctcgttctc ctcgacgctc cggtgcgtcg
     9181 agcggacctg gacctgcatg atcgcgagga gcggaatcgc gatcgcgagc gcgagcacga
     9241 cgaaccgcgg aaccggcgag cgaggccgga cgtcgttcag agagccctcc ccttacgaag
     9301 ggccctcagg agcaaggacc cgaatccgga tgcggactac gtcccgtacc ggtatttcgg
     9361 ctcccagccg ttcacgccat ccttgaggag cccgaacaga tgccatgcgg agcagaagga
     9421 atcgaagggc ccgaacccgg cgcgggccac gcggacgatg ctcccgtcgt cgttcttgta
     9481 gaacgtggag acgcccggga gccagctctt ctcgctccgg aatcccatgt cctcggtgaa
     9541 catcgagccg tggctggaga tcatgcggaa gttccagccg cgggaatgcg cgagctgcgc
     9601 ctgggtgtcg ggagaatccg gggtgatgac gacgaaggcg gcacggttct cgaggtgcgg
     9661 caggatgccg ttccaaccgt cggcccagag cgtgcagtag cggcagccct tccccatgtt
     9721 gtggatcacg atgaggtcct tgccctggcc gaagagctgc gacagcttca ccggaccgtg
     9781 gggaccggtc agggtgtaat cggagacggg agtcgtcggc agcaagcgct tgtgctcggc
     9841 cagccgctcc gagcgctccg cgatctcctg ctcgagccgc tcgatttcgt aggcgagatc
     9901 cgtcatttcc ttcatcgccc ctcctctaac ggtccggaca ccggcccgga aacccaggcc
     9961 ggcggcgccg gattcgacca tcgagcatac ccccggcgac ggggggcgca acggggaact
    10021 acggagaccc cggccgggat cgcgctatac tgttgccgtg aatgacgtcc gcccgggtcg
    10081 cccgacccag ccgccccgcc gcgccgcgac gatcgcaggg ctcgcggcac tcgcgctcgt
    10141 gtcgatgggc gccgccttcg aatcccactg gcaggacggg aaggccgagc tggacggcta
    10201 ccgctacacc gtcacccgct acggccacgc gcgggccggt accgcggtca tggtgtacgt
    10261 caccgagccc ttcagcgaag ccaagcgcgt gaaggtggat catcccacgc ccggctccgc
    10321 cgacgtggtc gaggcgctga agctcaactt catccgcgat ttccagaccg ggatctacga
    10381 ctacaacacg atggtgtccc tgttcgcgcg gagccgcgac ttctcgccga tgaagatctc
    10441 gttctcgggc tcggagtggt gcggcaacgt gtacgaggaa atgctcttcg agcgctacca
    10501 tctcgccgat cgctaccaga gctatttcga aggggagtcg gacgcgcgcc gcctgacgct
    10561 cgtgaagccc ggcatcgccg aggaggagct cttcatcctg ctccgtgatc tccggagtga
    10621 ttggctggcg ccggggcaga agcggaagat cgcgttcctt cccgcggcgt tctaccggcg
    10681 gctcacgcac agaccgttga tctggaccca agccgtgatc gagcgcggca agggcgtgga
    10741 agccgtgacc gtgccggcgg gacgattcga cgcggtgcgc tacgtggtaa aggtcgccga
    10801 cggacgcgag gggacgttct gggtggagcg cgcgtatccc caccgcatcc ttcgctggac
    10861 gtggctcccc gccgccggca tgacgcagcg aggcgggtgg tcgcctgcgg aagggctcga
    10921 ccagggcgag ctcgcgggaa gccagcgtct tccctactgg cagctccacg agcccggcga
    10981 tgagaagtac ctggcgcagc tcgggctcaa gccccagacc gccatcgtcg gcgggacgac
    11041 ccggctccgg ggcgacgtgg ggaccacgcc gtccaaacgg taggcgtccc caaacccccg
    11101 ttcagggacg cccccggtgc tgcacttcga tgatgggccg gacgcggccc tgggccgggt
    11161 agcggccccc tagaactgcg ccttctcggt gctcccgtcg agcgcgagcg tcgagcagct
    11221 tccgttcgag atgacctgca tcacctcgtc gaagtagccg gtcccgactt cccgctgatg
    11281 ccgcgtggcc gagtagccgt gcttctcgga gtcgaactcc gccgtctgga gctcggcata
    11341 gcccgccatt ccgcgatcct tgtacgcccg cgccagctcg aacatgccgt ggttgagcga
    11401 gtggaagccg gcgagcgtca cgaactggaa cttgtagccc atcgctccca gctccttctg
    11461 gaacttcgcg atggtctcgg gatcgagctt cgcgcgccag ttgaaggacg gtgagcagtt
    11521 gtaggcgagc atcttgcccg ggaacttggc gtgcaccgcc tcggcgaact gccgcgcctc
    11581 ctcgagatcg ggcgtcgacg tctccatcca gatgagatcg gcgtagggcg cgtaggcgat
    11641 cgcacgggcg atggcgcact cgatgccgcc cttgtagccg aagaatccct ccgacgtgcg
    11701 gttgccggtc aggaactccc ggtcgcgctc gtccacgtcg ctcgtgatga gcttggcgct
    11761 gtccgcgtcg gtgcgcgcga cgatgagggt cggcacgtcg aggacgtccg ccgcgaggcg
    11821 cgcggccacg agcgtgcgga tgaagtgtcc ggtcgggacg agcaccttgc cgcccagatg
    11881 gccgcacttc ttctccgagg agagctggtc ctcgaagtgc acggccgccg cgcccgcctc
    11941 gatcatggac ttcatgagct cgaacgcgtt gagcggtccg ccgaagccgg cctcggcgtc
    12001 agcgacgatg ggtgcgagcc aggtgcgcgc cgccggagcg ttgtgtccgt tggctccgtg
    12061 gccgttctgg gagtgaccgt tctgggacgg cgccttgcga gcttccgcgt gctcgatctg
    12121 gtccgcgcgc aggagcgcct ggttgatgcg gcgcaccacg ctcggaacgc tgttggcggg
    12181 atagaggctc tggtcgggat aggtctgccc ggcgaggttg gcatccgcgg ccacctgcca
    12241 gccgctcagg tagatcgctt cgagacccgc cttcacctgc tgcaccgcct ggttgccgct
    12301 cagagcgccg agcgcgttca cgtactcgcg cgtcgtaagg agatgccaga ggcgccgggc
    12361 gccgagctgg gcgagcgtgt gctcgacctt gaccgagccg cggagccgct cgacgtcctc
    12421 gttgcgatac ggacggacca cgccggccca gcgcgaagct gggagcggca tgagggccgg
    12481 ttccgccggg actgcgcgcg ctgcggcgac gttcgggaca gccgccggcg cgggcgcctg
    12541 cgtgatgcgg tttcgagctc gtttcaagcg cgtcgttgcc atgatgtccc ccttgatgtc
    12601 gacgactcga gccggatcgc tctctagtcg atccggctgt aagccggaat ggtcaaaaac
    12661 tcttcgagcg tctccgccgt ggagagctcg agaaacaagt cacacgcttc ggtgaatcgc
    12721 ccgttcctga atctctcgga accgatctcc tgctccacat ggcgcatctc gtcctcgacg
    12781 accgcgcgga catgcgcggc gtcgacgacg gctccgtctt cgaggcgcgc cttgtggtgg
    12841 acccactgcc acacctgcgc tcgggagatc tccgcggtcg cggcgtcctc catgaggttg
    12901 tagaggggga cgcagccgtg cccctcgagc caggcttcca ggtactggat cccgacccgg
    12961 gcgttgagcc tgaggccctc ttcggtgcgc gttccgcgcg gcacggcgag aaggtcgctc
    13021 cccgtgacct ccacgtcctc gcgcttccgg tcgagctgat tggggccgtc catgacggcc
    13081 tcgaacactt cgcgcgcgag cgccacgagc cccggatgcg cgacccaggt gccgtcgtgg
    13141 ccatccgtga cctcgcggag cttgtcctgc cgcaccttct cgagcgccgc gtggttcgcc
    13201 tccgggtcgc tcttgatcgg gatctgagcc gccatgcccc ccatcgcgtg cgcgccgcgc
    13261 cgatggcacg tgcgaatgca gagctgcgtg tacgcgcgga ggaagggctg cgtcatcgtc
    13321 acctgggagc gatcgggcag aacggccgcg gggtcgtttc gccgctgctt gatgaaggag
    13381 aagatgtagt cccagcggcc gcagttgagc ccggccacgt gctcgcgcag ctcgtagagg
    13441 atctcgtcca tctggaacgc ggccgggagc gtctcgatca gcaccgtggc cttgatggtt
    13501 ccgcgcggga cgtcgagcgt ctcctcggcg gcgaggaaga cctcgttcca cagacgcgcc
    13561 tcgcgatagc tctccagctt ggggagatag aagtacggtc cggttccccg ccggatcagc
    13621 tcgtgcgcgt tgtggaagaa gtagaggccg aagtcgaaca gcgctcccgg gacgggccgt
    13681 ccgtccacgt ggaggtgccg ctccgggagg tgcagcccgc gcgggcgaac gatgagcgtg
    13741 gctgtgcgat cggcgagccg gtactccttg cccgacgccg gatccacgta gtcgatcgtg
    13801 cggcgaacgg cgtccatgag gttgagctgc ccttcgacgt tgttgctcca ggtcggggcg
    13861 ttcgaatcct cgaagtcggc catgaagcaa tccgcgccgg aattgagcgc gttgatcacc
    13921 atcttccggt cgaccggtcc cgtgatctcg acgatgcgtc gcgtgagatc gccggggacc
    13981 ggcgcgatcg tccagtcgcc ttgccgaacc ggaagcgtct cgtcgaggaa ctcgagtggg
    14041 gcggcgatcg agagccggct ggcgcggtcc ccgagaagct gctcgagggt tggccggaac
    14101 gcccgcgtga ggctctcgag gaacgccagg gcctccggcg tgaggagccg ctcgtatcct
    14161 gggcggaccg gggcttcgac cgaagcccct gtcgatgtga gtgcgctcaa gttcatgtaa
    14221 caagaatgac agaatctgtg gatcaaagcc agtaatttat tgtgttacaa taaattactg
    14281 catttactaa tacctatttc accaatgcct tatttgtaac tgtggtaaac tgtgttggtg
    14341 gaagcgaact tgaaagcccc agccctgggg cggaagatcc gcgccctccg ccaggccgag
    14401 gggctgaccc aggccgccat ggccgcccgg ctcggaatct ctacttcgta cctgaacctc
    14461 atcgagaacg atcgccgccc gctgggtgcg ggactcctcc tcaccctggc tcgcgcgttc
    14521 aatatcgacc tcagaacctt cgccatcggc gaggatgcgc ggctcgtgtc ggacctcgcc
    14581 gaggccttcg gcgatccgct cttcggcgag cgccgcctcg acgagcggga gctccgcgag
    14641 ttcgtatcgt cgagtcccgc catcgcccgc gccgtggtgc agctccatca cgcatacacg
    14701 gacgcgcgcg catccgccga atccatcgcc gccgaggtgg tggaccggca ggatctctcc
    14761 ggagtggatc gcgcgggact cgcggccgag gaggtatcgg acctcgtcca gcgtcacgtg
    14821 aaccacttcc ccgagctcga agccgaggcg gatcggatct ggaaagacgc caagctcgag
    14881 gatcaggact tcttcgaggg aatggcgcac tacatggagc ggcgccacag cgtgcgcgtg
    14941 cgggtgctcc gcctgggtga gatgggggac gcggtgcggc gcttcgatcc ggaacggcgc
    15001 gagctgcatc tgtccgaggt gttgaggcgg ggctcgcgca acttccagct cgcgcatcag
    15061 gccgcgctcc tcacctgccc cgacactctg gatcgcatcg cccaggacga gcagctcacg
    15121 tcgccggagt cgcgcgcgct ctgccgggtc gcgctcgcga actacttcgc cggcgcgctt
    15181 ctcatgccct acgacgcctt ctttcgcgcc gccgaggaag agcgctatga cacggacctc
    15241 ctcgagcatc gcttccgcgc caacttcgag cagatctgcc atcgcctcac gacgctcaac
    15301 cgctccggag cgcgcgggat cccgttccac atggtgcggg tcgacatcgc cggaaacatc
    15361 tcgaagaagt tcagcgccac cgggcttcgg tttccgaggt tcggcggcct ctgcccgcta
    15421 tggaacgtgc acgcggcctt cctccagccg aacgtcatcc gcgcgcagct cgaccgcttc
    15481 cccgacggaa ccctctactt ctccttcgca cgcacgatcc ggaagcaccg cggcggctat
    15541 cacacgcccg aagtcttgta ttccgtgggc ctggggtgcg acgtggagtc ggccaagcgg
    15601 ctggtgtacg ccgacggttt cgatctcacg aatccgcagg ccgccgcgcc ggtcgggatc
    15661 acgtgccgcc tgtgcgagcg cctcgactgc cgggcgcggg cattcccctc gatctatcag
    15721 ccgctcaagg tggatgagaa tctgcggggc gtttcgttct tcgcgaggcc ggggggaacg
    15781 gggacgtagg gcggcgggcc ggctgttcat ggctttcacg cgagttgccg caagcaccct
    15841 actccgatcc gtgaacgaac gttgacgcta caggccccgg ggccagttgc tgagggtccg
    15901 ccgcgacgct cggaaggacc gatccagcgt ccccacgctc ccgccaactg gccccggggc
    15961 cagttgtctg agaccacaga cgggagcaac cgaggcccac tgtagtgccc cacgcactgg
    16021 ccccggggcc agtgatcgga gagcccgggg gagcaaccca gttccagccg gcgtccgacc
    16081 tactggcccc ggggccagtt gaaacgacgc acagccgtga atgcggacgt ggtggtgctc
    16141 gcgggttaat tcttcattca cattgggatg gcgagaatcc tggccagcaa cgattcggtt
    16201 ccactggccc cggggccagt tgaaaccacg cacaggcgtg accacggcgc ggtgcatggc
    16261 gaccatcgaa gcagcatgaa tgtgattgga cggagtgctg gcgtgaaggg agcaagcccc
    16321 gggtggagcg gggagtgggt gtgaagggag ccaagccccc ggcggagccg gccgggtcag
    16381 gcgtgggcga gctggtagac gatgagctgg gcgactgtcc agagcacgag cccgatgccc
    16441 gtgatgggaa ggacgagggc gagggcgaat cgaatgccga agccgcggac gctgaagtgg
    16501 cgcatggcgg aatcatcggc agatccggtg gaaacctaaa ggccacggca cgccgtcgca
    16561 ggccgcggcc agccgtggcg ggccgctcag cgctccgcgg aaccaaaccg ctcagcgtcc
    16621 cgcggtaccc gaccgctcag cgctccgcgg tacccaacct ccggtccacg acgacctgcc
    16681 ccgccttcat cacccaccgg acgcgcgcgg tgacggcctg gatgtccgtg agtggattcc
    16741 cctcgacggc cacgatatcc gccatgtagc caggcgccag tgcgccgagc tgcttctcct
    16801 gcccgaggag cgcggcgccg ttcacggtcg ccgcggcgag cgcctgcgcc ggggtcatgc
    16861 ccgccttcac gaaccactcg agctcgcgcg tgttctcgcc gcacatcgtg aagagggcgt
    16921 cggagcccat cgccaccttc accttcgccc gcacggcgcg gcgaagcgtc tccacatttc
    16981 gcccgcggta ctcgttcagc cgcgccaccg cggacgagtc gtagccgaag atcgaagcat
    17041 tctcggcgta atagcgattg tgatcgatcg tcggaacgta gaacgtgccc cgcttcgcca
    17101 tctcgcggag ggtggcatcg tccatgtcgg tcgcgtgctc gatcgacgtg gcgcccgccc
    17161 gaacggcatc gcgcgcgccg tcgggaccat acgagtggac cgcgacgcgc ttgccccgtc
    17221 ccttcgccgc gtcgaccgcc gccttaagct cttcatacgt gtaggtctcg tcgccggtca
    17281 cgtcgtccgc gcttccggtc gagccgtaga ccttgatcac atcggcgccg gccgcgatgt
    17341 tctgccgcac gacgcgcatc acctcggcgg ctccgtcggc gttcccgccc ggcggttcca
    17401 tgtagccggg gcggttcgga ctcagggtcg gatggagtcc gtacccgcag acgaacatcc
    17461 gcgggcccat catcgcgccg ctcgcgatga gatcgcgcat ggcgatgtcc atgtagtccc
    17521 aggagccgag gtcgcgcacc gtggtgacgc cggtctcgag cgtgtgtttt gcattacctt
    17581 gcgcgagata gaccgtcgcc gcggtgggcc gtgcggagag ctgctgccac ggattcgttc
    17641 ccggcgtgcc gtcccacgag taggtgaggt gggtgtgcac gtcgatcagt ccggggattc
    17701 cggtgtagcg cgtgaggtcg atctcctgcg cgccggccgg gagcgacgtt cgagccgcgg
    17761 gaccaatcgc gcggatcttg tccccctcga cgatcacgac cgcatcctcg atcacggggc
    17821 cggccccggt cacgatccgc gcgaagcgga gcgcgaacac gcgcggcacg gccggcacgg
    17881 cggacgcggc cggcacggca gatgcggccg gcgcagcggg cgcagcgggc gcggcagatg
    17941 ctgcgagcgc agcgggcgcg gcaaacgcga c
//
LOCUS       DASTKC010000052         7955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1694674, whole genome shotgun sequence.
ACCESSION   DASTKC010000052 DASTKC010000000
VERSION     DASTKC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694674"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFR25_02245"
     CDS             complement(<1..721)
                     /locus_tag="VFR25_02245"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9939900.1"
                     /translation="MTKTRVLLVLFGLALSAAAIVMLGRSIDVGEAMRAMGRASIPWF
                     LAANLLTIAVYWLRSLRWRSLLKPHASPTVGRLFRANMVGFLAINILPARLGELVRAF
                     ALARKERISTAAVLGSVAVERILDLVFLGIFWALSLLFAPLPPWFKWSGYATIAIAVV
                     VGGGIWFLNAARGRSSRLRSGVMAWVPHRMKDRFSASVSAFGEGIQIVGRPALLLEAA
                     FWSAASWLVSCGTFLLVGQSLG"
     gene            complement(718..1668)
                     /locus_tag="VFR25_02250"
     CDS             complement(718..1668)
                     /locus_tag="VFR25_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939901.1"
                     /translation="MSRHSEIDLKGVRTIPVAQRPTKVRTEQFAPAPDPDAPVGSFDQ
                     YLPDLLTGAALRRLVEAWVLARADGRPAVAMLGGHVIKTGVQRPLLALLEAGAFTAVA
                     MNGAAAIHDFEIALFGSTSEPVEETLGAGQFGLVEETPALMNLAVNEGVKREKGMGEA
                     LGDFLNEADAPHASLSLLARAAQLGIPCSVHVAIGTDTLHQHPSFDGASTGAATHRDF
                     RIFTAALRDLEGGLVLNFGSAVILPEVFLKALSTVRNLGHEAARFTAANFDQIRHYRP
                     EKNVLERPLLAGGHGHSFVGPHELWIPLLAALYLGRRPTA"
     gene            complement(1691..2569)
                     /locus_tag="VFR25_02255"
     CDS             complement(1691..2569)
                     /locus_tag="VFR25_02255"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET9939902.1"
                     /translation="MKRRFSWLGMAIGSGLLLLLPMRTTAPTAARADEPAATPIPAAT
                     SPASAEVAAAPDTADAGPQTGLAPVPWKIGEYFQFAIDWNGLNGGGSLMQVQNIVRVD
                     GRRAYRIVTKAESNSFVSKFYKVRDRAESFVDAESLYSVRFVKHLREGGYKKDVDVRF
                     DQGAGKARYDDGKAYDVPKRVHDVLSAFYYVRTVPLPDGATLSIPTHDNEKTYEMVVK
                     VHGREKVEVPAGKFDCVVVEPILKSEGVFKSKGSILVYLSDDARRIPVMVKSKIPIGS
                     IAVSLTDMRLSFPGRH"
     gene            complement(2613..3056)
                     /gene="dut"
                     /locus_tag="VFR25_02260"
     CDS             complement(2613..3056)
                     /gene="dut"
                     /locus_tag="VFR25_02260"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET9939903.1"
                     /translation="MTTVPLKITLEEHAVGAPEYQSEHAAGMDLVAAIDREVEIAPGE
                     VVAVPTGLRLEIPVGYEGQVRPRSGLAAKHAIGVPNAPGTIDADYRGEVKVLLVNLGR
                     TPYRVQRGDRIAQLVIAPVVRAAIDVVASLTTTARGDGGFGHTGR"
     gene            complement(3053..4411)
                     /locus_tag="VFR25_02265"
     CDS             complement(3053..4411)
                     /locus_tag="VFR25_02265"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9939904.1"
                     /translation="MATVVAGVRSLRAASGASASPVRLTAEDFTRVTLPNGITIVGER
                     MPAVRSVAVGAWIKVGSRHEVGPEAGVSHFLEHVVFKGTKRRDALAIALSLESVGGSL
                     DAFTGREVTCYNARVLEEHLDLAVDVIADLALSPRIDPADVEKEKGVILEEIHSSEDT
                     PDDRVHDLFADTVWRGHPLGHRILGDEETVSAFTPEMVAEYHGRHYTAPNLLLSVAGS
                     FDWDRFVGLVERHFVAGPGRPARSRAETSPNGGGVFHHAQDLAQQHLCIGGVGLPHEH
                     PDRFALVLLSSLLGGGMSSRLFQRVREQEGLAYSVYTYADSYDDTGLFCAAMSVQPDN
                     GPRALQLTLEEFDRAAQEGIPPDELDSVKMQLKGSLLLGTESTSSEMTRLARAEIYAG
                     RNVPVAELIESIDRVTEADVRRLAGELFKRERLSLVALGGRAERSYVAADLLDKSARG
                     AA"
     gene            complement(4368..6560)
                     /gene="pnp"
                     /locus_tag="VFR25_02270"
     CDS             complement(4368..6560)
                     /gene="pnp"
                     /locus_tag="VFR25_02270"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9939905.1"
                     /translation="MPESVSVELGGRTLSIEVGKVAKQASGATWVQYGETVVLNAAVA
                     SKDPIDKDFFPLSVEYREKTYAAGKIPGGFFKREGRPTEKEILSSRLIDRPLRPLFPD
                     GYRNEIQIMSTVLSSDQANDADILGLTGASAALMVSDIPFPEPVSAVRIGFIGGQLTL
                     NPTHAELVESELDLVVAATDSNIVMVEAGAREVSEATIVEALEFAHVAIRKINQIQHE
                     LRARVGKPKREFPLPKRNEALDRQIAETYRDRLQKANKISEKLERQAALDTLKVEAIE
                     LFEGTHPGSAKDVRRVLGELEERAVRETIVKEKKRPDGRGFDEMRKVNCEVGVLPRTH
                     GSALFTRGQTQALVVATLGTKVDEQRVEELEGQSWKSYMLHYNFPPYSVGEVRPFRGP
                     GRREIGHGALAERAIEPLIPSDIDFPYTIRIVSDILESNGSSSMATVCGGSLSLMDSG
                     VPIKTHVAGVAMGLVKEGNDFAILTDIQGVEDHLGDMDFKVAGTRNGVTALQMDIKIS
                     GVSYALMADALEKARRARMHLLDIMDAAIQTPRAELSPYAPRIYIMQIDPDKIREVIG
                     PGGKMIKKISAETGTQIDIEDSGEVRIAAFSGADGDRAREIIRSITEDPEIGRVYQGT
                     VRRVVPFGAFVEISPGKDGLVHISELETKRVERVEDVINEGDSVLVKVIGIDREGKIK
                     LSRKQALPGHENDPVVVSEPRGDRGGRGGRDGGRDGDRGRRRPEPSRR"
     gene            complement(6694..6963)
                     /gene="rpsO"
                     /locus_tag="VFR25_02275"
     CDS             complement(6694..6963)
                     /gene="rpsO"
                     /locus_tag="VFR25_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9939906.1"
                     /translation="MTLTPERKREVIGQFKTHETDSGSAEVQIALLTERIKYLTEHFK
                     VHKRDHHSRRGLLRMVGQRRRLLDYLKSTKIERYRLVVKELGLRK"
     gene            complement(7043..>7955)
                     /locus_tag="VFR25_02280"
     CDS             complement(7043..>7955)
                     /locus_tag="VFR25_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HET9939907.1"
                     /translation="PIALTIGVFDGLHRGHQEIVAATLAEARSRGGAAWVATFDPHPD
                     SVVRGTPDRPWITPPGERASLLHAMGVDRVETVRFDDEVRGLTPEGFLDRVLGPGAPL
                     QALVAGPDFHMGKGRVGDRGYLEEMGRKRGFVYREVAFLESGEGGGKLSSTRLRQEIA
                     AGAIDQAADIMGRPYALEGTVGSGAGRGTTLGFPTANLEVRPEKLLPAPGIYLSQNQF
                     DGRTWPGITYVGSAGTFGPGPVRVEVFLIDYNGALRGRWLKTLLISQLRADRVFRSAD
                     ELIRAMDDDLARARAFWAGARQADPAR"
BASE COUNT         1246 a         2720 c         2708 g         1281 t
ORIGIN      
        1 tgccaaggct ctgacccacc agcaggaagg tcccgcagct gaccagccac gaagctgccg
       61 accagaaggc cgcctccaag aggagggccg gccttccaac gatctggatg ccctccccga
      121 acgcggacac cgatgccgag aaccggtcct tcatccgatg gggcacccac gccatgaccc
      181 cgcttcggag acggctcgat cgtccgcgcg cagcattcag gaaccagatc ccacccccca
      241 cgaccaccgc gatcgcgatc gtcgcgtagc cgctccactt gaaccagggc ggaagcggcg
      301 caaacagaag cgacagcgcc caaaaaatac ccagaaagac gaggtcgagg atgcgctcca
      361 cggcgacgga tccgaggacg gcggccgtgg agattcgctc cttccgggcg agcgcaaagg
      421 cccggaccaa ttctcccagt ctcgcgggca ggatgttgat cgcgaggaag ccgaccatgt
      481 tcgcgcggaa gagccgtccg acggtcgggc tcgcgtgcgg cttcaggagg gagcgccagc
      541 ggagcgatcg gagccagtag acggcgatcg tgaggaggtt ggccgccagg aaccagggaa
      601 tggacgcgcg gcccatcgcg cgcatggcct cgccgacgtc gatcgagcgg cccagcatca
      661 cgatcgcggc cgcgctcagg gcgaggccga agaggacgag caggacccgg gtcttcgtca
      721 cgcggtggga cgccgtccga ggtagagcgc ggccaggagc gggatccaga gctcgtgcgg
      781 gccgacgaac gagtggccgt ggcctcccgc gagaagcggg cgctcgagca cattcttctc
      841 gggccggtag tgtcggatct gatcgaagtt cgccgcggtg aaccgggcgg cctcgtggcc
      901 gaggttgcgc accgtcgaga gcgctttcag gaagacctcg ggcaggatca ccgccgatcc
      961 gaagttgagc accaggcctc cttcgagatc gcggagcgcc gcggtgaaaa tccggaagtc
     1021 gcggtgcgtg gccgcgcccg tggacgctcc gtcgaacgac gggtgctgat ggagcgtgtc
     1081 cgtcccgatc gccacgtgca cgctgcacgg aatcccgagc tgggcggcgc gcgcgaggag
     1141 gctgagcgac gcgtgcggcg catcggcctc gttcaggaaa tcgccgagcg cctcgcccat
     1201 tcccttctcc cgcttgacgc cttcgttgac cgcgaggttc atgagcgccg gcgtctcctc
     1261 gacgaggccg aactgtcccg cacccagcgt ctcctcgacg ggctcggacg tgctcccgaa
     1321 gagcgcgatc tcgaaatcgt gaatggccgc cgccccgttc atcgcgacgg ccgtgaacgc
     1381 tcccgcctcg aggagggcca ggagcggccg ctgcacgccg gtcttgatga cgtggccgcc
     1441 cagcatcgcg accgcgggcc ggccgtcggc gcgcgcgaga acccatgctt ccacgaggcg
     1501 gcggagcgcg gccccggtga gcaggtcggg gaggtactgg tcgaaggacc cgacgggcgc
     1561 atcggggtcc ggcgcgggag cgaactgttc cgtccggacc ttcgtcggcc gctgggcgac
     1621 ggggatggtg cggacgcctt tgaggtcgat ctcggagtgg cggctcatcg cgcggccccc
     1681 ggcgccagcc tcaatgccgt cccgggaacg agagccgcat gtcggtgagg ctcaccgcga
     1741 tcgaaccgat cgggatcttg ctcttcacca tgacgggaat ccggcgcgcg tcgtccgaga
     1801 gatagacgag gatgctcccc ttcgacttga atacgccctc cgatttgagg atcggctcga
     1861 cgaccacgca atcgaacttg cccgccggga cctccacctt ctcccggccg tgcaccttca
     1921 ctaccatctc gtacgtcttc tcgttgtcgt gcgtcggaat cgagagggtc gctccgtccg
     1981 gaaggggcac ggtgcggaca tagtagaacg cggagagcac gtcgtgcacg cgcttcggca
     2041 cgtcgtaggc cttgccgtcg tcgtagcgcg ccttccccgc gccctggtcg aaccggacgt
     2101 ccacgtcctt cttgtagccc ccctcgcgca ggtgcttcac gaatcggacg ctgtaaaggg
     2161 actcggcgtc gacgaaggat tcggcccggt cgcgcacctt gtagaacttg gagacgaacg
     2221 agttcgattc cgccttcgtg acgatgcggt aggcccggcg cccgtccacg cgcacgatgt
     2281 tctgcacctg catgagcgag ccgccgccgt tcagtccgtt ccaatcgatc gcgaactgga
     2341 agtactcccc gatcttccac ggcaccggcg cgaggccggt ctggggcccg gcgtccgcgg
     2401 tgtccggggc ggcggccacc tcggcagacg cgggcgaagt cgcggcgggt atgggcgtgg
     2461 ccgccggctc gtccgccctc gccgccgtcg gcgcggtggt gcgcatgggc aggaggagca
     2521 ggaggccgga gccgatggcc attccgagcc aggagaatcg acgtttcata tcgagacaaa
     2581 cacgccgaat ggagccccgg gtccccagcg cctcaccgcc cggtatggcc gaacccgcca
     2641 tcgccgcgcg ccgtcgtggt gagcgaggcc accacgtcga tcgcggcccg gacgacggga
     2701 gcgatcacga gctgcgcgat ccgatcgccg cgctggacgc gatagggagt acggcccagg
     2761 ttcacgagca gcaccttgac ctcgccgcgg tagtcggcgt cgatcgtgcc gggagcgttt
     2821 ggaaccccga tggcgtgctt cgcggcgagg ccgcttcggg ggcgcacctg gccttcgtat
     2881 ccgacgggga tctcgagacg cagcccggtc ggaacggcga ccacctcgcc cggagcgatc
     2941 tcgacctcgc gatcgatggc cgcgacgaga tccatcccgg cggcatgctc cgactgatac
     3001 tccggcgcgc cgaccgcgtg ctcctcgagc gtgatcttga gcggaaccgt ggtcacgcgg
     3061 ccccccgcgc gctcttgtcg aggagatccg cggccacata cgaccgctcc gcccgcccgc
     3121 cgagcgcgac gaggctgagc cgctcgcgct tgaagagctc ccccgcgagg cggcgcacgt
     3181 ccgcctcggt cacccgatcg atcgactcga tgagctccgc caccggaacg tttcggcccg
     3241 cgtagatctc ggcccgcgcg agccgtgtca tctcgctcga cgtgctctcg gtgccgagca
     3301 ggaggcttcc cttgagctgc atcttcaccg aatcgagctc gtccggagga atcccctcct
     3361 gggccgcgcg atcgaactcc tcgagcgtga gctggagcgc gcgcgggccg ttgtccggct
     3421 gcacgctcat cgccgcgcag aagaggccgg tatcgtcgta cgaatcggcg tacgtgtaga
     3481 ccgagtaggc gagaccctcc tgctcgcgca cgcgctggaa gagtcgcgag ctcatgccgc
     3541 cccccaggag cgaggacaga agaacgagcg cgaaccggtc gggatgctcg tgcggcaggc
     3601 cgacgccgcc gatgcagaga tgctgctgcg ccagatcctg ggcgtgatgg aagacgccgc
     3661 cgccgttggg tgaggtctcg gcgcggctcc gagccgggcg cccgggcccg gcgacgaagt
     3721 gccgctccac gagaccgacg aagcgatccc agtcgaagct tcccgccacg gacaggagca
     3781 ggttgggcgc ggtgtaatgc cggccgtgat actcggccac catctccggc gtgaaggcgg
     3841 acacggtctc ctcgtcgccg aggatgcggt gtccgagcgg atgcccgcgc cagaccgtgt
     3901 cggcgaagag gtcgtggacg cggtcgtcgg gggtgtcctc ggagctatgg atctcctcga
     3961 ggatgactcc cttctccttc tccacgtcgg cgggatcgat cctgggggag agagcgagat
     4021 cggcgatcac atcgaccgcg aggtccaggt gctcctcgag gacgcgcgcg ttgtagcagg
     4081 tcacttcgcg gccggtgaac gcgtcgaggc tcccgccgac cgattccagg gagagcgcga
     4141 tcgcgagcgc gtcgcgccgc ttggtcccct tgaagacgac gtgctcgaga aagtgggaga
     4201 ccccggcttc gggaccgacc tcgtgccggg atccgacctt gatccaggcc ccgaccgcaa
     4261 cggagcgaac cgcgggcatc cgctccccaa cgatggtgat cccgttgggg agcgtgaccc
     4321 gcgtgaaatc ttcggcagtc aaccgcaccg gcgacgccga ggcgccgcta gcggcgcgaa
     4381 ggctccggac gccggcgacc acggtcgcca tcccggccgc cgtcccgtcc gccgcgaccg
     4441 ccgcgatcgc cgcgcggctc ggacacgacc accggatcgt tctcgtgacc cggcagcgcc
     4501 tgcttgcggc tcagcttgat cttcccttcg cggtcgatgc cgatcacctt gacgaggacc
     4561 gagtccccct cgttgatgac gtcctcgacg cgctccaccc ttttcgtctc gagctcggag
     4621 atgtggacga gcccgtcctt gcccggcgag atctcgacga acgcgccgaa cggcacgacg
     4681 cgtcgcaccg tgccctggta gacgcggccg atctcgggat cctcggtgat cgagcggatg
     4741 atctcccgcg cgcggtcgcc gtcggcgccc gagaaggcgg cgatccggac ctcgcccgag
     4801 tcctcgatgt cgatctgcgt gccggtctcg gccgagatct tcttgatcat cttgccgccc
     4861 ggaccgatga cctcgcggat cttgtccggg tcgatctgca tgatgtagat gcgcggcgcg
     4921 tacggcgaaa gctccgcgcg cggcgtctgg atggcggcat ccatgatgtc gaggaggtgc
     4981 attctcgccc ggcgcgcctt ctcgagcgcg tccgccatga gggcgtagga gacgcccgag
     5041 atcttgatgt ccatctggag ggccgtgacg ccgtttcgcg tgcccgcgac cttgaagtcc
     5101 atgtcgccca ggtgatcctc gacgccctgg atgtccgtga ggatcgcgaa gtcgttcccc
     5161 tccttgacga ggcccatcgc gacgcccgcg acgtgggtct tgatcggcac gcccgagtcc
     5221 atcagcgaga ggctgccgcc gcagaccgtg gccatcgacg acgagccgtt cgactcgagg
     5281 atgtcggaga cgatgcgaat cgtgtacggg aagtcgatgt cgctcgggat cagaggctcg
     5341 attgcgcgct cggcgagcgc gccgtgcccg atctcgcggc ggcccgggcc ccggaaggga
     5401 cggacctcgc cgaccgagta gggcgggaag ttgtagtgga gcatgtagct cttccagctc
     5461 tgcccctcga gctcctccac gcgctgctcg tccaccttcg tgccgagcgt ggccacgacc
     5521 agcgcctgcg tctggccgcg cgtgaagagc gcggagccgt gcgtgcgcgg aaggacgcct
     5581 acttcgcaat tcaccttccg catctcgtcg aagccgcggc cgtcaggacg cttcttctcc
     5641 ttgacgatcg tctcgcgaac ggcgcgctcc tcgagctcgc cgagcacccg ccgcacgtcc
     5701 ttggccgagc ccgggtgggt tccctcgaac agctcgatcg cctcgacctt gagggtgtcg
     5761 agggcggcct gccgctcgag cttctccgag atcttgtttg ctttctgaag gcggtcccga
     5821 taggtctcgg cgatctggcg atccagggcc tcgttgcgct tcgggagcgg gaactcgcgc
     5881 ttcggctttc cgacgcgcgc gcggagctcg tgctggatct ggttgatctt ccggatggcg
     5941 acgtgggcga actcgagcgc ctcgacgatc gtcgcttccg acacctcgcg cgcccccgcc
     6001 tccaccatca cgatgttcga atcggtggcc gcgaccacga ggtcgagctc cgactcgacg
     6061 agctcggcat gggtcgggtt gagcgtgagc tggccgccga tgaagccgat gcggaccgcc
     6121 gagaccggct cggggaacgg aatgtccgag accatgaggg ccgccgaggc gcccgtgagt
     6181 ccgaggatgt cggcgtcgtt ggcctggtcc gacgagagga ccgtggacat gatctggatc
     6241 tcgttgcgat agccgtccgg gaagagcggc cgaagcggcc ggtcgatgag ccggctcgac
     6301 aggatctcct tctcggtggg acggccctcg cgcttgaaga agccgcccgg gatctttccg
     6361 gcggcgtagg tcttctcgcg gtattcgacg gacagcggga agaagtcctt gtcgatgggg
     6421 tccttgctcg cgaccgccgc gttcagcacg acggtctcgc cgtactggac ccaagtggcc
     6481 cccgaggcct gcttggcgac cttcccgacc tcgatggaca gggtgcggcc gccgagctcg
     6541 acgctcactg attcgggcat tcgtttctcc tctctgaaac tccatgactc tcgaacggca
     6601 tcgggcgccc ctggcgagcc attggccagg cgaggcgcct cgggagtgtc ctagggagga
     6661 attgcggcga ggttcccgct cgcgcgggag gagctacttg cggaggccaa gctccttgac
     6721 caccaaccgg tagcgctcga tcttcgtcga tttcaggtaa tcgagcagcc tacgtcgctg
     6781 acctaccatc cggagcagtc cccggcgact gtgatggtcg cgcttgtgga ccttgaagtg
     6841 ctccgtcagg tacttgattc gttcggtgag gagggcgatc tggacctcgg ccgaccccga
     6901 atccgtctcg tgggtcttga attggccgat tacttcgcgc ttcctctcag gcgtcagcgt
     6961 caccggtgcc acctccttga ctttccctct ctgtgtacga gcgagcactc tagcacaggg
     7021 gggtggggtt tcaagggcat tcctaccgag ccgggtctgc ctgtcgggcg ccggcccaga
     7081 aggcgcgtgc gcgggcgaga tcgtcatcca tcgcccggat cagctcatcg gccgatcgga
     7141 acactctatc ggcgcggagc tgggaaatca gcagcgtttt gagccatcgg cccctcaaag
     7201 cgccgttgta atcgataaga aacacctcca cgcgcacggg cccggggccg aaggtcccgg
     7261 cgcttcccac gtaggtgatg cccggccagg tccggccgtc gaactggttc tgggagaggt
     7321 agatccccgg cgccgggagg agcttctcgg gcctcacttc gaggttcgcg gtcgggaagc
     7381 caagcgtcgt cccccgcccc gctccggagc ccaccgtccc ctcgagcgcg tagggcctcc
     7441 ccatgatgtc ggccgcctgg tcgatggccc cggcggcgat ctcttgcctg agccgcgtgc
     7501 tcgagagctt gcccccgccc tctcctgact ccaagaaagc aacctcgcgg tagacgaagc
     7561 cgcgcttccg ccccatctcc tcgaggtagc cccggtcgcc gacccggccc tttcccatgt
     7621 ggaagtccgg accggcgacc agggcctgga gcggcgcgcc gggaccgagc acccggtcga
     7681 ggaatccctc gggggtgagg ccgcggacct cgtcgtcgaa ccgcaccgtc tcgacccggt
     7741 ccacccccat cgcgtgaagg agggacgcgc gctcgccggg cggtgtgatc cacgggcgat
     7801 cgggcgtgcc ccgtaccacg gagtcgggat ggggatcgaa cgtggcgacc caggccgctc
     7861 cgccgcgcga acgcgcttcg gccagggtgg cggcgacgat ctcctgatgg ccgcggtgaa
     7921 gtccgtcgaa gacgccgatc gtgagcgcga tcgga
//
LOCUS       DASTKC010000053        24848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1223255, whole genome shotgun sequence.
ACCESSION   DASTKC010000053 DASTKC010000000
VERSION     DASTKC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 24848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24848
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223255"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VFR25_02285"
     CDS             <1..1424
                     /locus_tag="VFR25_02285"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9939908.1"
                     /translation="SLSKQFTAAAIYKLESQGKLHLTDTLGKWFPGAPGDKQDITIDQ
                     LVHHTSGLPYLGRGDLYDSVSVDSMVRETFSYPLDFAPGARYSYSSPGYDLLGVLIAR
                     ASGRSFNEYMRQEIFAPAGMTETGFVDETARWTGSKRTPSYSSVDPDPDPPLYPAALA
                     PKVVGAGGIISTTGDLWKWEQALRSGRVLDAPTTAKLFAPGVAMSPTSSYAGGWLVVR
                     SQRNTTVIMHGGDIGGFNADMRRMVDEHATIIFLSNTRDAGRGYRDVVPYTVTRVLFG
                     PEPTLPPAPVHVPESALRRWNGPVPVAPGVTVDARTHGGQVWLTARTQEGMFALAGAD
                     SMARAQSLTLNRLAETTTLPLLSGEATGLDSIFSPSLVETAHPYFFQLWKAVADSIGG
                     VTSHEVLGSTVSPPSGARSLVRVTGPKGSRVMTLDWLGGKLIQSSPIPGEGLTFRFQP
                     ESDETLSRYDLWAGRVIRVARRG"
     gene            1429..1884
                     /locus_tag="VFR25_02290"
     CDS             1429..1884
                     /locus_tag="VFR25_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9939909.1"
                     /translation="MADTPDPVTANERIRAARERAGLEHGEMADKVGISWEEYWDIEG
                     FEEDVWCCHSFDTLHRLAQALGLTLLGILEGDDSVDPAESMSFADFRSAVAESVRASG
                     GDADAWGERAGWDVEPVLRDPEAFWKRCPDELRDVANAAGIDWRAVLPK"
     gene            1895..2461
                     /locus_tag="VFR25_02295"
     CDS             1895..2461
                     /locus_tag="VFR25_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9939910.1"
                     /translation="MATVLLFHHALGLTPGVQAFADELRAAGHTVHTPDLYEGKTFTE
                     LNEGVAHADRTGFDTITDRGVSAADQLPADIVYAGFSLGAMPAQKLTQTRPGARGALL
                     FHSSIPLKYYGGSWPAGVPLQIHTMEKDDWGDVDVAKQIAETVPGTELFLYPGDRHLF
                     TDRSTPDYDEKAAAQVRERVLQFLGKVG"
     gene            2466..3107
                     /locus_tag="VFR25_02300"
     CDS             2466..3107
                     /locus_tag="VFR25_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET9939911.1"
                     /translation="MRVGILGSGLMGGKLGTLFARAGHDVVFSYARQRAKLEGLARSA
                     GGKARAGTPAEAAACDAVLLAVHWSRLDDVLHQTGDLSGKVVVSCSLPMSEDDSGLVI
                     AHTSSGAEELAKRLPRAQVVLAFNTVPSEVLFDVFDARDKGPRPSLAYCGDHEHAKTV
                     AATLIRDVGFDPVDAGPLKIARYMEPFTLLIAQLAYEGEKGPELAYRFEWRNG"
     gene            3104..3910
                     /locus_tag="VFR25_02305"
     CDS             3104..3910
                     /locus_tag="VFR25_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939912.1"
                     /translation="MKDLDRLTIVAIAVLAYSLANIVHEGLGHGGACLLLGARPTMFN
                     AIFFNYDETTASDTVQRLISAAGSIVNVIVGLPIVALLLRKRSGMAPALRYFLWLFAA
                     VNLLTAFGYLLYSGVGGIGDWARVIQGAEPVLLFRGVMSAVGAWLYFIVAPRLLMPAL
                     DPFLGVNPVERPRRAKTLMLLPYFIGAATLVVAGIFNPLGVQIVLISAAAASLGGTSL
                     LAWYHAKPRTPSALAQDPPLGVPRSLGWIAAAVVVLAIFVFVLGPGIGKL"
     gene            3988..4542
                     /locus_tag="VFR25_02310"
     CDS             3988..4542
                     /locus_tag="VFR25_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9939913.1"
                     /translation="MANGDATAARLWLWARKARATDWDARFAEELPRVYNFFRYRVGP
                     GVVAEDLTGITFEKAWSARDRYREDRAGFETWLFAVARNVAVDHFRRAKVSVPLDEVV
                     DLPGGPTPEEIAERRSDEARLAALLARRSERERELLALKYGAELTNRDIARMTGLSES
                     NVGTILHRAVEALRDDWNKESHDG"
     gene            4535..5515
                     /locus_tag="VFR25_02315"
     CDS             4535..5515
                     /locus_tag="VFR25_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939914.1"
                     /translation="MDDRMLHEYRREPDPEFARDLRERLRRSERTRRFPRPVLRAVAV
                     ACSLGVVVALFAIPSVRVSAQALLDLFRVRRFAAVNFDQSRLDALQSIQKEKGFLVFD
                     HEEMIREPGPPRSVPTAQAATAEAGFAVSSPSYLPDSLAVDSVLVQGAGAMRFSVSEA
                     KLRSVLDKLDVKGVTVPSGLDGQWVEVKKPPIVIQRFQSSNRRAVLLQALSPEIAVPA
                     GWNIEQLAEIGLRILGLDPSEAKRIARTTDWRTTLLVPVPLNATTFRLVTVRGNSGLM
                     IITTGDEAPAPGKPKRKGAMVMWSEGDRVFCLRGDLSAENLLQMANSVSP"
     gene            5528..6466
                     /locus_tag="VFR25_02320"
     CDS             5528..6466
                     /locus_tag="VFR25_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9939915.1"
                     /translation="MTDPVIETRELRKQFGDKTAVEDLSLEVHRGEVFGFLGPNGAGK
                     TTSIKMLLGLVEPTSGSGSVLGAPIGHRATRARIGFLPEHFRFHDWLTGRELLLVHGR
                     FLGMSSRDTKARADALLARVDLLDAAHRPVRTYSKGMMQRVGLAQALLGRPDVVFLDE
                     PTSGLDPLGRLLVRDVIRELREHGTTVFLNSHILGEVEATCDRVTFVREGRTIRDLRL
                     GEIDGSTEVDLRVGSLTEEARAAVARIGRIVEDGATSTADGPERLRVSIPREDLLPEL
                     ARALVERGIALYEMRVARKSLEATFLEVMGEDQRPG"
     gene            6463..7302
                     /locus_tag="VFR25_02325"
     CDS             6463..7302
                     /locus_tag="VFR25_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9939916.1"
                     /translation="MSVAHGLLAVTRLTLYEAFRKRILMATLIAGAGFLTLYGLGLHF
                     MVRGEAGGMALVERRVFLNMLTLAGLFAANFLTIMTAVLLPVDTLSGEIASGVIQTVA
                     VRPIRRRDIVLGKWLGHWIVMAGYFGLLAGGVLTITFLRSGFTPPKLEIGLPLILLEG
                     TVLLTLSIAGGAKLSTVTNGMLAFGLFGLAFIGNLVEQIGTMAQNDAARQVGTVASLI
                     MPSESLWQLAAYHMQPSVIRDLGNSPFSPVSVPSVAMVVWAAGYIVVTLALGIRWFGK
                     RAL"
     gene            7543..7950
                     /locus_tag="VFR25_02330"
     CDS             7543..7950
                     /locus_tag="VFR25_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939917.1"
                     /translation="MGDSVLTCCGHYDPGFINERVIHVMYYDPTFQEKSWNIEILADA
                     ATQGAVYTLPTTVVPPHKVPYVTLFAPSGEYSSSEQLASGTITLNSFECGASTISVDF
                     SVDATLESETSHGSTITVQGRFRAVYPKTECEP"
     gene            7977..8531
                     /locus_tag="VFR25_02335"
     CDS             7977..8531
                     /locus_tag="VFR25_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939918.1"
                     /translation="MLVGAVILFTSCSSSKQVADTPTPAAPPAPASQAPARRAPEVPL
                     VAVARVYLDRGSNLHVVQVDSQDIRVTFQGRYLDPQLSPDRHTVGVLAADSVDNGVGD
                     TVEVANKLKLLRGGGFVQEFEPGGFIRSWSFARDGKAVAIYSGGLHFAGTYELYDTVS
                     GAMLEYAKDPVTDTSPDWVRALSP"
     gene            complement(8549..9019)
                     /locus_tag="VFR25_02340"
     CDS             complement(8549..9019)
                     /locus_tag="VFR25_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939919.1"
                     /translation="MDNPADVIRRLREQAFHLSAADLELSPTPARPHVWCAIMELGYP
                     TGIATLLSVAEGTTSLYFSNGGGVIGAGEHESVRDAAEVFLDAVEAHRAMLSPVGDTP
                     TPRIGRVRFYARTFEGTLGAEATEDLLGQKLHPLSPVFFAGHAVITAIRESSES"
     gene            9303..10094
                     /locus_tag="VFR25_02345"
     CDS             9303..10094
                     /locus_tag="VFR25_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939920.1"
                     /translation="MLLLAPGPKASAATSFSFSLRVGDPYIGPRLAFYEQPDVVLVPG
                     TRVYYVDDYDYDLYRYGNYWYYCWNGGWYRSVDYDGPFYFIGYQSVPYSIRYIPVRYR
                     HHWRDYRGAAYYSYRAGRYYPSNPTSYTYYRTRYENQYPRQSRGQYSVQQNRYRQQQP
                     VQQYRQQRQFRQQQAPQYRQQQAPQQRERYQRQQQPQRLQQLPERVQQPPQSVQQQPE
                     QRPQQASQGQRNRGGGNRGGGSQKERDQKEHGQGNGNGHGRGQGG"
     gene            10355..11461
                     /locus_tag="VFR25_02350"
     CDS             10355..11461
                     /locus_tag="VFR25_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HET9939921.1"
                     /translation="MTNSRLAELRHEIDLLNENILASIQRRGSIVLEIAALKRSLGIA
                     GYDAKREEEMLQSLTSSALGPFGPAEVKEIFKAIFRVSLDLQNLERRKGMRVLRRDLV
                     PESGFLVGDVRIGGPEPVLFLGPCSVETAEQIDTIAEFVAGIPGPKVLRAGAFKPRTN
                     PYTFQGLREEGLRLLRNAADRHGLVVVTEVLDTATVDIVSEHADMLQIGARNMYNTEL
                     LKAVGRAGKPVLLKRAFMATTDELLLSAEYILAGGNDRVALCERGIRTFQRTTRFTLD
                     IAAVPLLQQETSLPVIVDLSHALGRKDIMLPCARAALAVGANGLMIEIHNRPDQALSD
                     GFQQFDLKEFAAFLDNLGHRAAAVREPVLGGRNG"
     gene            11454..12230
                     /gene="cmoA"
                     /locus_tag="VFR25_02355"
     CDS             11454..12230
                     /gene="cmoA"
                     /locus_tag="VFR25_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HET9939922.1"
                     /translation="MDRTLRTRRAAGEATSARDQIFAAPAERVADFDFGEKTAAVFDD
                     MLGRSVPFYAEIQRMTGELAADYAAEGTAIYDLGCSTGTTILEIAEHIPRERNVRYIG
                     VDNSPEMLEKARRKLAESEFDRPFELEFADLNAGVRIDNASVVLMVLTLQFIRPMYRH
                     RLIRSIYEGLTENGALILVEKVLGENSEFNRLFIEHYYEMKRENGYSDLEIAQKREAL
                     ENILVPYRLEENKELLREQGFRHVDVFFKWYNFCAIIATK"
     gene            12194..12988
                     /locus_tag="VFR25_02360"
     CDS             12194..12988
                     /locus_tag="VFR25_02360"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9939923.1"
                     /translation="MVQLLRDHSDQVAPSAVHRTLVRFGEFLFSGRNLIFPFAFLAIA
                     AVSFPRAPFESERADLILDAVGLSVAVAGQTLRAVVIGLAYIRRGGKGKKIHADRLVQ
                     DGIFAHSRNPLYVGNMMVFLGLFIVLNSTWGYLVGVPFFYISYLSITLAEEEFLRKRF
                     GEEYVDYCRRVNRFLPSWKGIAATMRSMSFDWGRLIRKEYGSTFIWITAALWLLLWEA
                     AVRQGWDSVRPRARVLAAVWVVALAAYLVARYLKKTGRLSAQRAAG"
     gene            13029..13814
                     /locus_tag="VFR25_02365"
     CDS             13029..13814
                     /locus_tag="VFR25_02365"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HET9939924.1"
                     /translation="MDFLFDLPLIVTGPLLLAVLVGFSLLGLSWFRKHRLPHLRFGET
                     DVEFGAAMLASIMVFYGLATALTAVQVWEGYEKVKEINEQEASSLATFYRNVTYYPEP
                     IRSAIRERIRDYTYQVIHESWPLQHLGQIPTQGVRAMDEVQAMLVRFEPSTEAEKGLA
                     LETLASYDRMMEARRMRLGSVERKLPGILWLVIVAGAFISLISSFYFPVLDPGVHRLQ
                     VGLLAGFIGLVIFMMLALDRPYRGDLGLKPRPYELLYEQLMTR"
     gene            complement(13878..15005)
                     /locus_tag="VFR25_02370"
     CDS             complement(13878..15005)
                     /locus_tag="VFR25_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9939925.1"
                     /translation="MKSYQSTTATYQQGARTLPGEFYTSERVLAEERERVFARGWNCV
                     GRASRMARPGDFLVRDVAGESILIVRDREGVLRAFFNICRHRGTRICREESGQLGATI
                     QCPYHAWTYSTDGRLVGAPHMQDVAGFDKRDYPLHAAALAEWEGFLFVHLGENPTPFE
                     VAWAPLQGKFARFGLPGLVVGHSVSYEVRANWKLVFQNYSECLHCPTIHPKLATVLPY
                     QSGANDLTEGPFLGGYMEIKPPNESATMSGRACGHLVCGDLPAEDRRRGFYYSLMPNL
                     LLSLHPDYVNYYLIRPLAVDRTLVESEWIFHPDTVADATSNIRDAIEFWDLTNRQDWD
                     IVEQSQLGVSSRRYTPGPYSPRESVPAAWDREYLRQMDRDL"
     gene            complement(15015..16604)
                     /locus_tag="VFR25_02375"
     CDS             complement(15015..16604)
                     /locus_tag="VFR25_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9939926.1"
                     /translation="MSAGTYDVVVIGGGANGLTAAATLGRAGRRVLLVEGDAELGGQA
                     RVIEFAPGFRAAPLGQDPGWIPETVARALGMNGLERLAADPGIAVRADGTYLTLPRDA
                     ARAADAIRVHSPSDAAKWPEFTSRLRKLAGFLETLYTTPAPDISASSLGELLPLLSVG
                     RKFRALGRRDMVEFLRTLPLSAWELADDWFECAPIKAAIAAAGVQHHQQGPRSGGTGF
                     VLLHHLIGAPAGALRGRSPWRSGPGAFAKAAEAAARRQGVEIRTNARVGRVEVRDDAV
                     AGVVLRNGEEIAARAVLSTANPAATLLDWVDPVWLDPEFLLAVGNIRHRGSTAFVLYA
                     LDVLPDFPGLSSKEALAGIVSLTPSVREIEQAADAAKFGMVSEHPHVELWAPTLHWPD
                     LAPGRHVIVARAQHAPYRLRDGAEWNRTAREDLANRVTAAIEGAAPAFSAHVLHHAVL
                     TPRDLEERFGLREGAFSQGELGLDQILFMRPIAGWGNHATPITGLYLGGAGTHPGPGI
                     LGGAGWLAGRQLLADLRRRSQ"
     gene            complement(16601..18184)
                     /locus_tag="VFR25_02380"
     CDS             complement(16601..18184)
                     /locus_tag="VFR25_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9939927.1"
                     /translation="MSAPRYDAIIVGGGHNGLVAAAYLARAGRRTLVLERRPVLGGAA
                     VTEQVFPGFKFSVFSYVVSLLRPEIIRDLDLPRHGLQILPLESTVTPLDNGDYLASWA
                     DPDETRRELCRHSTRDADATILFGRLMHHMAMAVKPILGMVPPDPASLSPKDLMGLLK
                     LGRHFRGLGPERFHALHKLMTMSSADYLDEWFEFGPLKATKSASGIIGTFLGPRSPGS
                     AYVLLHHYMGEIDGAFRAWGFQKGGTGGISEAIAGAARAHGAEIRTSAAVEHVLVREG
                     RAIGVVLAGGEEIPASTVISGLDPRLTFTRLVDPNELPDPLVEGIRRFKFRGSSGKVN
                     LALSGLPDFTCLPGHGPHLRGAISISPSIEYLERAYDDAKYGEFSRNPYMDIVIPSMI
                     DPGMAPPGKHVMSIFVQYAPYTLNGGWNDQRREAFGDAVVKTLARYAPNIESLILHRQ
                     VLTPDDIERITGLSEGNIFQGELALQQLFFLRPVPEWAKYRTPIQGYWQCGAGTHPGG
                     GIMGASGRLAALEILRSRA"
     gene            complement(18181..19326)
                     /locus_tag="VFR25_02385"
     CDS             complement(18181..19326)
                     /locus_tag="VFR25_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HET9939928.1"
                     /translation="MRAQTWRRWAGYQMASAYDPHPDREYAAIRNSAALIDVSPLHKY
                     RITGRDAERLLDRMITRDMTKLREGQVYYTPWCDHAGKVIDDGTVTRLGSSEFRLTSA
                     DSSLRWLHMNASGMEVGIEDISESMGAMSLQGPLSRAVLSQASPADLGSLKYFRMVPT
                     TVRGIPVAISRTGYTGDLGYEIWVDAAQAVNLWDALVEAGTSYGVTPAGVWALDIARI
                     EAGLIMLDVDYFSSHHALIEARKSTPYEINLGWAVSADKGPFNGRRALAAERTRGAAW
                     GFVGLEVDWVGFERLFAARGLPPQVPSIAWRASAPIYKDGRQVGYATSGCWSPLLKKL
                     LALAHLRAPHFAPGTSVSLEVTVEHHRERVDAVVRKLPFYDPPRKKA"
     gene            complement(19548..20492)
                     /locus_tag="VFR25_02390"
     CDS             complement(19548..20492)
                     /locus_tag="VFR25_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HET9939929.1"
                     /translation="MTPGRRFDRERAPVRLLSVIAILGLGVAMSSCSQKPAEQQTFAT
                     PEAAVEAMFHALSEGDTTALHALLGPDASDVMNRADPVQAARDRQVVIAAMIERWWLE
                     GEGPTRTVVVGNESYPLPIPLVEEKGRWRFDTAAGKEEMLYRRIGRNELAVMDVAEAF
                     VEAQHEYAATGHDGVPKGAFAQRMLSQPGRHDGLFWPATPASPAPSPMGELAAKAAAE
                     GYRRQEGPAPYEGYFFKVLTAQGPSADGGERSWIVGSRMTGGFGLLAWPADYGKSGVM
                     TFLIGPDAKLRQQDLGAETLARAEAINSFDPDSSWSVP"
     gene            complement(20489..21628)
                     /locus_tag="VFR25_02395"
     CDS             complement(20489..21628)
                     /locus_tag="VFR25_02395"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HET9939930.1"
                     /translation="MISRSHPLVALAALCLVAFALYPASAPASDADNLDKLLAPIALY
                     PDALLAQILTASTSPDQVTEFSKWLKDQTATGSDLQQAAMNAKFDAAFASLAIFPDVV
                     KLLADNMDWTKEIGTAYLSSKDQVNTSIQRLRAQAKEKGNLKSNEQQKVEVNSEGGSQ
                     TIIIEPANPQVVYVPQYDPNVVYTQSASSSSSNDAAVAALVGFTLGIAIGAAMSNDYY
                     GWGAWGYGWHGGGAYYHRSVWVVPARPRYPYARPVPNYRPRGNVATPRNTNVNRNGNS
                     NRTNAGNRVDSKDVDRRDASRDKAKAGDRGGAKTDRESSTARGRPSTNDKPGRPESDA
                     FGGYGNGSSTRASSDRGRSSVASSKGGGSSRGGGRSGGGGGGRRR"
     gene            21934..23154
                     /locus_tag="VFR25_02400"
     CDS             21934..23154
                     /locus_tag="VFR25_02400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9939931.1"
                     /translation="MLRTVRCVFLAVLILASTFPARTALALGAQEVGSILSIRPLGEA
                     EPLPGDDGLTHVAYELHVVNPSRFLVSIDLLEILDAGNGRTLGRLAGSALSAMTIMGG
                     GEKGPVFGPSHSGYIYLDVVLPNEVLPSAIDHRFTISLQVRAAADDDHHGGPVPASVG
                     LDSSLTFTGARVEIDRRPPAVIAPPLRGDHWLVANGCCDAVNPHRAAVIAIDGALYVP
                     ERFAIDFVQLDRDGKLLTGPVDKLTSYSYFGAPVFSVASGTVAAAHDGEPEEVPGAMP
                     KGKTISTATGNYVIVDIGSRRYAVYAHFKTGSVRVRAGQHVAEGDVLGLVGNSGNTSN
                     PHLHFHVSDRASPLSSVGLPYVFKRFEDQGIVSGLDAVIGGGAGTIQRAPQPGPHRNQ
                     LPLDNHVVGFGPTN"
     gene            23370..>24848
                     /locus_tag="VFR25_02405"
     CDS             23370..>24848
                     /locus_tag="VFR25_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HET9939932.1"
                     /translation="MTRLTTPSYPRWTRTLLVLALLILGAEPASAQQSRNVTLLSHMD
                     LYSMAGYSSCWSYIHSDGREYAVEFSRSGASIVRLADPQNPVEVAFFNLPDSEWHEGR
                     QYQHYFYITTEFVNNYNPGGLTILDMADPEHPRRISNFDSPILWSHTIEIDTARGFLY
                     TPGAAGTGPSGNIVQGLFILSLANPENPTVIAVYGDEAPNYTHTIHVRGTRGYASMQN
                     QGTVRILDLTNPAQPSLLAEIQTPAGVEPGGDRTLFRRTHSAWESADERFLYVSDETS
                     PLGVYVYDIQNLANIQRVYTFEGMPPRSIGHDPVVRGNRLFLSYYTTGARVYDISNPA
                     WPVEIGYYDTYDGRDGGFHGCWEVAPLFPSGIFVASDMETGLYVFRLDATYGIVRGTV
                     RKGPNGPPVAGALVRQAGSSASTVSFSDGRYALAVTPGSNVTLNTTLFGYDPLSKSLA
                     VTAGSDRPLDLPLRASDAGSISGVVRGTPGSPTLNEAELEITG"
BASE COUNT         4129 a         8242 c         8171 g         4306 t
ORIGIN      
        1 gctcgctctc caagcagttc accgcggcgg ccatctacaa gctcgagtcc cagggcaagc
       61 tccacctgac cgataccctg gggaaatggt ttccgggcgc gcccggcgac aagcaggaca
      121 tcacgatcga ccagctcgtc catcacacgt cgggacttcc gtacctcggg cggggcgacc
      181 tctacgattc cgtgtcggtg gactcgatgg tccgcgagac cttctcgtac ccgctcgact
      241 tcgcgccagg cgcgcgctac tcgtactcga gtcccggtta cgacttgctc ggggtcctca
      301 tcgcccgcgc ctcgggtcgc tccttcaatg agtacatgcg tcaggagatc ttcgccccgg
      361 ccggcatgac ggagaccggc ttcgtcgacg agaccgcgcg ctggaccgga tcgaagcgca
      421 cgccgtccta ctccagcgtg gatcccgatc ccgacccgcc gctctatcct gccgcgctcg
      481 ctccgaaggt cgtgggcgca ggcggcatca tctcgacgac gggcgatctc tggaagtggg
      541 agcaggcgct tcgctcggga cgcgtgctcg atgcccccac gacggccaag ctcttcgcgc
      601 cgggagtggc gatgagcccg acctcgtcgt acgcgggcgg gtggctcgtg gtgcggagcc
      661 agagaaacac gaccgtcatc atgcacgggg gagacatcgg cgggttcaac gcggacatgc
      721 ggcgcatggt cgacgagcat gcgacgatca tcttcctgtc gaacacgcgc gacgcagggc
      781 gcggatatcg ggacgtcgtc ccgtacacgg tcacgcgggt tctcttcgga ccggagccca
      841 cgctgccgcc ggcgcccgtc cacgttcccg aatcggcctt gcgacgctgg aacggtccgg
      901 ttccggtggc gcccggcgtg acggtggacg cccgcacaca cggcggccag gtctggctga
      961 ccgcgcgaac gcaggaagga atgttcgcgc tcgcgggggc ggactccatg gcacgcgccc
     1021 aatcgctcac gctcaaccgc ctcgccgaga ccacgacgct tccgctcctt tcgggtgaag
     1081 cgactggact cgactcgatt ttcagcccga gcctcgtcga gaccgcccat ccgtacttct
     1141 ttcagctctg gaaggcggtc gcggactcga tcggcggcgt gaccagccac gaggtgctcg
     1201 gcagcaccgt gtcgccgccc agcggagcac ggagcctcgt ccgcgtcacc ggccccaagg
     1261 gctcgcgcgt gatgacgctc gactggctcg gaggaaagct catccagagc tcgcccattc
     1321 ccggcgaggg gctgacgttc cgcttccaac ccgagagcga cgagacactc tcgcgctatg
     1381 acctctgggc ggggcgcgtc atccgcgtcg cccggcgcgg ctgagccgat ggctgatacc
     1441 ccggaccccg tgacggcgaa cgagcggatc cgcgcggcgc gagagcgcgc aggcctggag
     1501 cacggcgaaa tggcggacaa ggtcgggatc agctgggagg aatactggga catcgagggg
     1561 ttcgaggaag acgtttggtg ttgccactcc tttgacaccc tgcatcgtct cgcgcaggca
     1621 ctgggtttga cccttctcgg aatcctcgag ggagacgact ccgtcgatcc cgccgaatcc
     1681 atgtcgttcg ccgatttcag gagcgcggta gccgagtccg tccgagcctc aggtggggac
     1741 gccgatgcct ggggcgagcg tgctggatgg gacgtggagc ccgtgctccg ggatcccgag
     1801 gcgttctgga agaggtgccc cgatgagctg cgcgacgtcg ccaatgccgc aggcatcgac
     1861 tggcgcgcgg ttcttccgaa atagggaggc ctctatggct accgtccttc tcttccatca
     1921 tgcgcttgga ctcacgcccg gcgtacaggc gttcgccgac gagcttcggg cagcggggca
     1981 caccgtccat acgccggacc tgtacgaagg caagaccttc accgagctga acgagggcgt
     2041 ggcccacgcc gacagaaccg ggttcgacac gatcaccgat cgcggcgtca gcgccgcgga
     2101 tcagctgccc gcagacatcg tctacgccgg cttttcgctg ggggccatgc cggcgcagaa
     2161 gctcacccag acgcgccccg gcgcgcgcgg agcgcttctt ttccacagca gcatcccgct
     2221 caagtactac ggtggctcct ggcccgcggg cgttccgctc cagatccaca ccatggagaa
     2281 ggacgactgg ggcgacgtgg acgtcgcgaa gcagatcgcg gagaccgtgc ctgggacgga
     2341 gctcttcctc tatccaggcg accgccatct gttcacggac cggagcacgc ccgactacga
     2401 cgagaaggct gccgcgcagg ttcgggagcg cgtcctccaa ttcctcggga aggtcggata
     2461 ggcgcgtgcg cgtcgggatc ctgggctcgg ggctcatggg ggggaagctc ggaaccctgt
     2521 tcgcccgcgc cgggcacgac gtcgtgttca gctacgcgcg ccagcgggcg aagctcgagg
     2581 ggctcgcccg gtccgcggga gggaaggcgc gcgcgggcac gccggcggag gccgccgcat
     2641 gcgatgccgt cctcctggcc gtccactggt cgcgcctcga cgacgtgctt catcagacgg
     2701 gcgacctgtc gggcaaggtc gtcgtgagct gctcgcttcc catgagcgag gatgactccg
     2761 gcctcgtcat cgcccacacc tcctcgggag cggaagagct ggcgaagcga ttgccccgcg
     2821 ctcaggtcgt gctcgcgttc aacaccgtgc cgagcgaggt gctgttcgac gtgttcgacg
     2881 cccgggacaa gggtccgcga ccgagcctgg cctactgcgg cgaccacgag cacgccaaga
     2941 ccgtggccgc gacgctgatc cgcgatgtag gcttcgatcc tgtggacgca ggtccgctca
     3001 agatcgcgag gtacatggag cccttcacgc ttctcatcgc gcagctggcg tacgaggggg
     3061 agaaggggcc ggagctggcg taccgattcg agtggcggaa cggttgaagg atctcgatcg
     3121 gctcacgatc gtcgccatcg ccgtcctcgc gtattcgctc gccaacatcg tccacgaagg
     3181 actcggccat ggcggcgcgt gcctcctcct cggggcgcgc cccacgatgt tcaacgcgat
     3241 cttcttcaac tacgacgaga ccacggcgag cgacacggtc cagcgtctca tctccgccgc
     3301 gggaagcatc gtcaacgtca tcgtgggctt gccgatcgtc gccttgctgc tgcgaaagcg
     3361 ctccggcatg gcgccggccc tgcgctactt cctctggctc ttcgcggccg tgaacctcct
     3421 caccgcgttc ggctatctgc tctactcggg agtcggcggg atcggggact gggcgcgcgt
     3481 gatccagggc gcggagccgg tccttctctt ccggggagtc atgtccgccg tcggagcctg
     3541 gctctacttc atcgtggcgc cccggctctt gatgcccgcg ctcgacccgt tcctcggagt
     3601 caatcctgtc gaacgaccgc ggcgtgcaaa gacgctcatg ctcttgccgt atttcatcgg
     3661 cgcggccacg ctggtcgtgg cggggatctt caatccgctg ggggtgcaga tcgtgctgat
     3721 ctcggcggcc gccgcgtcgc tcggggggac ctcgctcctc gcgtggtatc acgcgaagcc
     3781 tcgaacgccc tcggccctgg ctcaggatcc gcccctgggt gtcccgcgca gcctggggtg
     3841 gatcgcggcc gcggtcgtcg tgctcgcgat cttcgtcttc gtgttgggtc ccgggatcgg
     3901 aaagctgtag tcagtcccgc gcccccctgg accactggga aagaaaatct gtgatacctg
     3961 ccccgctcgc gcgtatccgg aggtgccatg gcgaacggtg acgcgaccgc ggcgcgcctc
     4021 tggctctggg ccaggaaggc gcgcgcgacc gactgggatg cgcgattcgc ggaggagctg
     4081 ccgcgggtct acaacttctt ccggtaccgg gtcggcccgg gcgtcgtggc cgaggatttg
     4141 accggaatca cgttcgagaa ggcctggagc gcgcgggatc gatatcgcga ggaccgcgcc
     4201 ggattcgaga cgtggctctt cgcggtggct cggaacgtgg ccgtcgatca cttccggcga
     4261 gcgaaggtgt ccgtgccgct cgacgaggtg gtcgaccttc cgggcggccc cacgcccgag
     4321 gagattgcgg agcggaggtc cgacgaggcg cgcctcgcgg ccctgctcgc acgacgatcc
     4381 gagcgggagc gcgagctcct cgcgctcaag tacggcgccg agctcaccaa ccgggacatc
     4441 gcccggatga ccggcctcag cgaatccaac gtcggcacga tcctccaccg tgcggtggaa
     4501 gcgcttcgcg acgactggaa caaggagtca cacgatggat gatcggatgc ttcacgagta
     4561 ccgccgcgag ccggaccctg aattcgcgcg cgatctgcgc gagcggctgc gccggagcga
     4621 gcgcacgcgc cgattcccgc gccccgtgct gcgcgcggtg gccgtagcct gctcgctggg
     4681 ggtggtcgtg gccctcttcg ccattccgtc ggtgcgcgtc tccgcccaag cgctcctgga
     4741 tctcttccgc gtgcggcgct tcgcggccgt gaacttcgac cagtcgcggc tcgatgcgct
     4801 tcagtcgatc cagaaggaga agggcttcct tgtcttcgat cacgaggaga tgattcgcga
     4861 gccgggcccg ccccggtccg ttccgaccgc ccaggcggcc acggcggagg ccggattcgc
     4921 ggtgagcagt ccctcctacc ttccggacag cctcgcggtc gacagcgtcc tcgtgcaggg
     4981 tgcgggggcg atgcgcttct cggtgagcga ggcaaagctc cgctccgttc tcgacaagct
     5041 ggacgtgaag ggggtgacgg tgccgagtgg gctggacggt cagtgggtcg aggtgaagaa
     5101 gccgcccatc gtgatccaga ggttccagtc ctcgaatcgc cgcgcggtcc tgctccaggc
     5161 gctgagcccc gagatcgcgg tgcccgcggg atggaatatc gagcagctcg cggaaatcgg
     5221 gcttcggatc ctcgggctcg acccgtccga ggcgaagcgg atcgcgcgca ccaccgactg
     5281 gcggaccacg ctcctcgttc cggtgccgct caatgcgacc acgttccggc tggtgaccgt
     5341 ccgcggcaac tccggactca tgatcatcac gaccggggac gaggcgcccg cgccgggaaa
     5401 gcccaagcgc aagggggcga tggtgatgtg gagcgagggc gatcgagtgt tctgcctgcg
     5461 cggcgatctc tcggcggaga acctgcttca gatggcgaat tccgtgtcgc cgtgacatcc
     5521 tgacggcgtg accgatcccg tcatcgaaac gcgcgagctg cggaagcagt tcggcgacaa
     5581 gaccgcggtc gaggaccttt ccctcgaggt gcatcgggga gaggtcttcg gcttcctcgg
     5641 accgaacggg gccgggaaga cgacctcgat caagatgctc ctgggactcg tggagccgac
     5701 gtccggatcc ggctccgtgc tcggagcgcc catcggtcat cgcgccaccc gcgcgcggat
     5761 cggcttcctg cccgagcact tccgattcca cgactggctc accggacgcg agctcctcct
     5821 cgtgcacggc cggttcctcg ggatgagctc ccgcgacacg aaggcgcgcg cggatgcgct
     5881 cctcgcgcgc gtcgaccttc tcgacgcggc ccaccggccg gttcgcacct actccaaggg
     5941 gatgatgcag cgcgtgggtc tcgcgcaggc gctcctcggg cgtccagatg tcgtgttcct
     6001 cgacgagccc acgtccgggc tcgatccgct cggacgcctc ctcgtgcgcg acgtgatccg
     6061 cgagctccgc gagcacggca ccacggtctt cctgaactct cacatcctgg gcgaggtgga
     6121 ggcgacctgc gatcgggtca ccttcgtgag ggaggggcgc acgatccgcg acctcaggct
     6181 cggggagatc gacggttcca cggaggtgga ccttcgcgtg ggatcgctca cggaggaggc
     6241 ccgcgccgcg gtcgcccgga tcggccgcat cgtggaggac ggcgcgacgt ccaccgcgga
     6301 cggccccgag cgcctgcgcg tctcgattcc gcgggaggac ctgcttcccg agctcgcgcg
     6361 cgccctggtc gagcgcggca ttgcgctcta cgagatgcgg gtggcgcgca agtccctcga
     6421 ggccacgttc ctcgaggtga tgggggagga tcagagaccc ggatgagcgt cgcccacgga
     6481 cttctcgccg tcacgcgcct cacgctgtac gaggcgttcc ggaagcggat tctcatggcc
     6541 acactgatcg ccggcgccgg attcctcacg ctctacggtc tggggctcca cttcatggtg
     6601 cgtggagagg cgggcggaat ggcgctcgtc gagcggcgcg tcttcctcaa catgctcacc
     6661 ctcgcgggac tcttcgccgc gaatttcctg accatcatga ctgcggtcct gctgcctgtg
     6721 gacacccttt cgggggagat cgcctcaggc gtgatccaga cggtcgcggt gcggccgatc
     6781 cgccgccgtg acatcgtgct cggaaagtgg ctggggcact ggatcgtgat ggcgggctac
     6841 ttcgggctcc ttgcgggggg cgtgctgacg atcacgttcc ttcgctccgg gttcaccccg
     6901 cccaagctcg agatcggact gccgctcatc ctcctcgaag gcaccgtcct gctcacgctg
     6961 tcgatcgcgg gcggcgccaa gctcagcacc gtgacgaacg ggatgctcgc ctttggcctc
     7021 ttcgggctcg ccttcatcgg caatctggtc gagcagatcg gcacgatggc gcagaacgac
     7081 gccgcgcgcc aggtcgggac ggtggcgagc ctcatcatgc cgagcgaatc cctctggcag
     7141 ctcgccgcat accacatgca gccctcggtg attcgcgatc tcgggaactc gccgttctct
     7201 cccgtctcgg tgccgagcgt ggcgatggtc gtgtgggccg cgggatacat cgtggtgacg
     7261 ctggccctcg ggatccgctg gttcgggaag cgggcgctgt agggagcacc tgaggtagac
     7321 tcatctgtcg agcctcaacg aggtggacag ggggtcgaga tgaacgttac tcgggtgtgc
     7381 gcgctccgca tccagctctt ggtcttgggt agcctggcca tcctcccggg ttgcagtgat
     7441 gacgaccaca gcagcaaatc gatgactccg acgggcccgc cgccgactcc cttcgtcgag
     7501 gactccctgg tctttcggac gtcgggggga tctgtcatcg agatgggaga ctccgtcctc
     7561 acgtgctgcg gccactacga ccccgggttc atcaacgagc gcgtcattca cgtgatgtac
     7621 tacgacccca cgttccaaga gaagtcgtgg aacatcgaga tcctcgcgga tgccgccacg
     7681 cagggggccg tctacacgtt gccgacgacg gtcgtgcctc cgcacaaggt cccgtatgtc
     7741 accctcttcg ccccctccgg cgaatacagc agcagcgagc agctggcttc cgggacgatc
     7801 acgctgaact ccttcgagtg cggggcctcg acgatatccg tggatttcag cgtggacgcg
     7861 accctcgaga gcgagacgtc ccatggatcc acaatcaccg tccaggggcg cttccgcgcc
     7921 gtctacccca agaccgagtg cgagccctga acgtcctcgg aagggtgcaa gcctgggtgc
     7981 tggtcggcgc cgtgatcctg ttcacctcct gcagctcgtc caagcaggtg gcggatacgc
     8041 cgactcccgc cgctccgccg gcgcccgcgt cacaagctcc ggcgcgtcgt gccccggaag
     8101 ttccgctcgt cgcggtcgcg agggtctatc tcgatcgagg cagcaacctc cacgtggtgc
     8161 aggtcgactc tcaggacatc cgtgtgacct tccagggacg ctacctggat ccccagctca
     8221 gcccggatcg acacacggtg ggcgtgcttg cggccgactc cgtcgacaac ggcgtcggcg
     8281 acacggtcga ggtagcgaac aagctcaagc tcctccgtgg cgggggattc gtgcaggaat
     8341 tcgagccggg gggcttcatc cgatcctggt cgttcgccag ggacggcaaa gcagtggcca
     8401 tctacagcgg aggccttcac ttcgcaggaa cctacgagct ctatgacacg gtgtccggcg
     8461 ccatgctcga atacgccaag gaccccgtta cggatacatc gccggactgg gttcgggcgc
     8521 tctcaccgta gacgaggccg gcgacttcct aggattcgct cgactccctt atcgccgtga
     8581 tcacagcatg gcccgcgaag aagacgggcg acaatggatg gagcttctgg cctagcagat
     8641 cctcggtcgc ctccgcgcca agcgtcccct cgaacgtgcg ggcgtagaat cgaacgcgac
     8701 cgatacgcgg cgtgggtgtg tccccgacag ggctcaacat agcccggtga gcttcgaccg
     8761 cgtcgagaaa cacctcggcg gcgtcgcgca cggattcgtg ctcgcccgcg ccgatgacgc
     8821 cccctccatt gctgaagtag aggctcgtcg tgccttcggc gaccgacagc aacgttgcga
     8881 ttccggtcgg atacccgagc tccatgatcg cgcaccagac atggggccga gcgggagtcg
     8941 gggatagctc caggtcggcg gcgcttaggt ggaaggcctg ctcgcgcagg cgccggatca
     9001 cgtcggcggg gttgtccatg gccgagatcc taccaagtca gacggggaag gctccgtgtc
     9061 gtccactggt cgcagtgtcg ctgtaatgta ctgacttcct atcccccaaa acgctcgaat
     9121 tcgaaacacc tgatagccct cgctgtatct gcctcattcc caaccatatc gcgacgtccg
     9181 attcgactcc ttccatggca caggcgttgc acaagtcacc tttcggtaat tcacgacgaa
     9241 tatccagaaa ggtgggaact cctatgagat atcgatcact ctgggccgcg ttcgcagcgt
     9301 tcgtgctgct gctcgctccg ggcccaaagg cttcagcagc gacgagcttc tcgttcagcc
     9361 tgcgcgtcgg cgacccgtac atcggcccac gcctcgcatt ctacgagcag cccgacgtcg
     9421 tgctcgttcc gggcacgcgg gtgtattacg tcgatgatta cgactacgat ctctatcgct
     9481 acggcaacta ctggtactac tgctggaacg gagggtggta ccgctcggtc gactacgacg
     9541 ggccgttcta ctttatcggc tatcaatccg tgccttactc cattcgctac attccggtgc
     9601 ggtacaggca tcactggcgg gattaccgag gcgcggccta ttactcctac cgggcggggc
     9661 gctactaccc gagcaacccg accagctaca cgtactatcg gacgagatac gagaaccagt
     9721 acccgcgtca gtcgcgcggc cagtactcgg tgcagcagaa ccgctaccgt cagcagcagc
     9781 ctgtgcagca gtatcggcag caacggcagt tccgacagca gcaggcgcca cagtaccggc
     9841 agcagcaggc gccacagcag cgcgagcggt atcagcggca gcagcagccc caacgcctgc
     9901 agcagctgcc cgagcgagtg cagcagccgc cacagagcgt gcagcagcag cccgagcagc
     9961 gcccgcagca ggcgtctcag gggcagcgaa atcggggtgg cggaaaccgt ggtggtggga
    10021 gtcagaagga gcgggaccag aaggagcacg gccagggcaa tggcaatggc cacggtcgcg
    10081 ggcagggagg ctgagaaagc cgactagcta cgagattaag gaacgggagg gcgcagcccc
    10141 acggggcatg cgccctcttc acattttagg gctgaggagt cggagctgcc ccttgtgctg
    10201 cgtccgcttc gttcccagcg gggcaaactc ccgatgtgga cgggagccgc cggacggatt
    10261 atcttctact gacgcggcgc tacggcaagg gcacgccagc gtcaacatcg cgtcccaggg
    10321 gtggggccgt acggctccaa cgacgaggtg ctcgatcacg aattcacgac tcgctgagct
    10381 gcgccatgag atcgaccttc tgaacgagaa catcctcgcc tccatccaga ggcggggctc
    10441 gatcgtcctc gaaatcgctg ccctcaagcg gtccctcgga attgccggat acgacgcgaa
    10501 gcgcgaggag gagatgctgc agagcctcac ctcctccgcc ctcggaccct ttgggcccgc
    10561 cgaagtgaaa gagatcttca aggcgatctt ccgggtctcg ctcgacttgc agaatctcga
    10621 gcggcggaag ggaatgcgcg tgctccgcag ggacctcgtg ccggaatcgg ggtttctggt
    10681 cggtgacgta cggattggcg ggccggagcc ggtcctcttc ctgggccctt gctccgtcga
    10741 aacggcggag cagatcgaca ccatcgccga gttcgtcgcc ggcattcccg gcccgaaggt
    10801 cctcagggcg ggggccttca agccgcggac gaatccatac accttccagg gtctgcggga
    10861 agaggggctg cggctcttgc ggaacgccgc agatcgccac ggcctcgtcg tcgtcacgga
    10921 agtcctggac accgccaccg tcgacatcgt ctcggagcac gccgacatgc ttcagatcgg
    10981 cgctcggaac atgtacaaca cggagctcct caaagccgtg gggcgcgccg gcaagccggt
    11041 cctcttgaag cgcgccttca tggccacgac cgatgagctg ctcctctcgg cggaatacat
    11101 cctggccggc ggcaatgatc gcgtggccct ctgcgagcgc ggcatccgca cgttccagcg
    11161 gaccactcgg ttcaccctcg acatcgcggc cgttcccctg ctccagcagg agacctcgct
    11221 gccggtgatc gtggacttga gccatgcgct cggccggaag gacatcatgc tgccctgcgc
    11281 gagggcggcg ctggctgtgg gcgcgaacgg gctgatgatc gaaattcaca accggccgga
    11341 ccaggcgctc tcggacggat tccagcagtt cgaccttaaa gaatttgcgg cgtttctcga
    11401 caacctgggc catcgtgccg ccgcggtccg ggaaccggtt ctgggaggac ggaatggata
    11461 gaaccttgag gacgcggcgc gccgccggag aggcgacgag cgcgcgggat cagatcttcg
    11521 cggcaccggc cgagcgggtc gccgacttcg acttcggcga gaaaacagcc gcggtcttcg
    11581 acgacatgct gggacggtcc gtcccgttct acgccgagat ccaacggatg accggggagc
    11641 tggcggcgga ctacgccgcc gaggggaccg cgatctacga cctgggctgc tcgaccggca
    11701 ccacgatcct cgagatcgcg gagcacattc cccgcgaacg gaatgtccga tacatcggag
    11761 tcgacaattc gccggagatg ctcgagaagg cgcggcgcaa gctcgccgaa tcggagttcg
    11821 accggccgtt cgagctggaa ttcgccgacc tgaacgccgg ggtgcgcatc gacaacgcgt
    11881 ccgtcgtcct catggtgctc acgcttcagt tcatccggcc catgtatcgc catcgcctga
    11941 tccggagcat ctacgaaggt ctcacggaga acggagcctt gatcctcgtc gagaaggtgc
    12001 tcggggagaa ctcggaattc aaccggctct tcatcgaaca ctattacgag atgaagcgcg
    12061 agaacggcta cagcgacctc gagatcgccc agaagcgcga ggcactcgag aacatcctcg
    12121 tcccgtaccg tctcgaggag aacaaggagc tcctgcgcga gcagggtttc cggcacgtgg
    12181 acgtcttctt caagtggtac aacttctgcg cgatcatagc gaccaagtag cgccgtccgc
    12241 cgtccaccgc acgctcgtgc ggttcggcga attcctcttc tcagggcgaa atctcatctt
    12301 tccgttcgcc ttcctcgcga tcgccgcggt gtcgtttccg cgtgcaccct tcgagagcga
    12361 acgggcggat ctcattctgg acgcggtcgg gctgagcgtc gccgtcgccg gacagacgct
    12421 ccgcgcggtg gtcatcgggc tcgcgtacat ccggcgcggc ggcaagggta agaagatcca
    12481 cgcggaccgc ctggtccagg acggcatctt cgcgcactcc cgaaacccgc tctacgtggg
    12541 caacatgatg gtatttctgg gtctcttcat cgtgctgaac tcgacctggg gctatcttgt
    12601 cggggtgccg ttcttctaca tctcctatct gagcatcacc ctcgccgagg aagagttcct
    12661 gcgaaagcgg ttcggggagg agtacgtcga ttattgccgt cgcgtgaacc gattccttcc
    12721 gtcatggaag ggcatcgccg ccacgatgcg gagcatgtcc ttcgattggg ggcgcctcat
    12781 ccgaaaggaa tacggctcga cgttcatctg gatcacggcc gcgctctggc tgctgttgtg
    12841 ggaggccgcc gtccgtcagg ggtgggattc ggtccgaccg cgtgcacgcg tcctggccgc
    12901 ggtttgggtt gtggcgctcg ccgcgtacct ggtggcgcgc tatctcaaga agaccgggag
    12961 gctgtcggct caacgcgcgg ccgggtgacc catcccgagc cacggcatct cttggaggag
    13021 ggcgcgcctt ggactttctc ttcgatctcc ccctgatcgt gaccggaccc ctcctgttgg
    13081 cggtcctggt cggcttctcg ctcctgggcc tcagttggtt tcgcaagcac cggctgcctc
    13141 atctgcgatt cggtgagacc gacgtggagt tcggcgccgc gatgctggcg tccatcatgg
    13201 tgttctatgg cctcgcgacc gcgttgaccg cggtgcaggt ttgggagggc tacgagaagg
    13261 tcaaggagat caacgagcaa gaggccagct cgctggcgac cttctaccgg aacgtcacgt
    13321 attaccccga gccgatacgg agcgccatac gcgagcggat tcgcgactac acctatcagg
    13381 tcatccatga gtcctggccg ctccagcacc tgggccagat tcccacccag ggcgtgaggg
    13441 ccatggacga ggtccaggcc atgctcgtgc gcttcgaacc ctccaccgag gcggagaagg
    13501 ggctggcgct cgagaccctc gcgtcctacg accggatgat ggaggcccgg cgtatgcgcc
    13561 tgggctcggt ggagcgcaag ctgcctggaa ttctatggct ggtgatcgtc gcgggagcct
    13621 tcatcagcct gatctcttcg ttctactttc cggttctcga cccaggcgtc caccgcctcc
    13681 aggtcggcct gctcgcgggg ttcatcggcc tggtgatttt catgatgctc gccctcgatc
    13741 gaccctaccg aggcgatctc ggtctgaagc caaggccgta cgagctcctg tacgaacagc
    13801 tgatgacgcg ctgaccgaac agcaaaggga acagccgcga ggacggttgg gtcgggcagg
    13861 agtagtcgtg gatgctgcta caaatctcga tccatctgcc gcaggtattc gcggtcccac
    13921 gccgccggga cgctctcgcg tggggagtaa ggtcccggtg tataccggcg ggacgagaca
    13981 ccgagctggc tctgctcgac gatgtcccaa tcttggcgat tggtgaggtc ccagaactcg
    14041 atcgcgtccc ggatgttgct cgtcgcgtcc gcgacagtat ccgggtggaa gatccactcc
    14101 gactcgacca gcgtgcggtc gacagctagg ggtcggatga ggtagtagtt cacgtaatca
    14161 gggtggaggc tcaggagcaa gttcggcatc agcgaatagt agaagccgcg tcgccggtct
    14221 tccgcaggga gatccccgca aacaaggtgc ccgcacgcgc gtccgctcat cgtcgcgctc
    14281 tcgttcggtg gcttgatctc catgtatccg cccaggaacg gcccctcagt gaggtcgttg
    14341 gcgccgctct ggtacgggag cacggtcgcg agcttgggat gaatcgtggg gcaatggagg
    14401 cactctgaat agttctggaa cacgagcttc cagttcgcgc ggacttcgta ggacacggag
    14461 tgtcccacga cgaggcccgg aaggccgaag cgcgcgaact tcccctggag cggtgcccat
    14521 gcgacctcga agggagtggg gttctctccc aagtggacga acaggaatcc ctcccactcg
    14581 gcaagcgccg ccgcatggag cggataatcc cgcttgtcga agccggcgac gtcctgcata
    14641 tggggcgccc ccacgagccg cccgtccgtg gaataggtcc acgcgtggta cgggcactgg
    14701 atcgtggctc cgagctgccc ggactcctcg cgacagatcc gcgttccgcg gtgccggcag
    14761 atattgaaga acgcccggag aactccctcc cggtcgcgca cgatgaggat cgattccccc
    14821 gcgacgtccc ggacgaggaa atcgccgggc ctcgccattc gcgaggcgcg cccgacgcaa
    14881 ttccacccgc gcgcgaacac gcgctcccgc tcctcggcca ggactcgctc ggacgtgtag
    14941 aactcgccgg ggagtgttcg cgctccctgc tggtacgtcg cagtcgtgct ctgataggac
    15001 ttcatgggcc tcgtctactg actccggcgg cggagatccg cgagtagttg ccgcccggcg
    15061 agccaaccgg cgccccccag gattccaggg ccggggtggg ttcccgcacc acccaagtat
    15121 agccccgtga tcggcgtggc gtggtttccc cacccggcga tcggacgcat gaacaggatc
    15181 tggtcgagcc ctagttctcc ctgggagaag gcgccctcgc ggaggccgaa tcgctcctcc
    15241 aggtcacgcg gcgtgagcac cgcgtggtgg agcacatgcg cactgaatgc aggagccgcc
    15301 ccttcgatgg cggcggtcac tcggttcgcc aaatcctccc gagcagtccg attccactcg
    15361 gcgccgtcgc ggagccggta gggcgcgtgc tgggcgcgcg cgacgatgac gtggcgcccc
    15421 ggcgcgagat cgggccagtg gagcgtggga gcccagagct cgacgtgcgg atgctccgag
    15481 accatgccaa acttcgctgc gtccgcagcc tgttcgatct cacgcacgga tggtgtcagc
    15541 gacacgatgc cggcgagcgc ctccttcgag gagagcccgg ggaaatcggg gagcacgtcc
    15601 agcgcgtaga gcacgaacgc ggtggagccc cggtggcgga tgttccccac ggcgaggaga
    15661 aattcgggat ccaaccagac cggatcgacc cagtcgagaa gggtagccgc ggggttcgcg
    15721 gtggagagca cggcgcgcgc cgcgatttcc tcgccgtttc gcagcacgac gcccgcgact
    15781 gcgtcgtcgc ggacttcgac tcgccccacg cgcgcgttag tccgaatctc caccccttgc
    15841 cggcgcgctg cagcctctgc cgccttcgcg aacgcaccgg gtccactccg ccagggtgag
    15901 cgcccgcgca aagcgcccgc gggagccccg atcaggtgat ggagcagcac gaagcctgtg
    15961 ccgccggaac ggggaccctg ttggtgatgc tggacgccag cggccgcgat ggcggccttg
    16021 atcggcgcgc actcaaacca gtcgtcagcc aactcccacg cggagagcgg gagcgtgcga
    16081 aggaattcca ccatgtcgcg ccggcccaga gcgcggaact tccgccccac ggagaggagc
    16141 gggaggagct cgcccagcga ggaggcgctg atgtctggag cgggcgttgt gtaaagcgtc
    16201 tcgagaaacc ctgccagctt ccggagccgc gacgtgaact caggccactt ggccgcatcg
    16261 gaaggcgagt ggacgcggat cgcgtccgcg gcccgggccg cgtctcgagg aagggtgagg
    16321 tacgtcccat ccgcgcggac cgcgatcccc ggatcggcgg cgaggcgttc gagcccgttc
    16381 atgccgagcg cacgcgccac ggtctcggga atccagcccg ggtcctggcc gagtggggct
    16441 gcgcggaacc caggtgcgaa ctcaatcacg cgcgcctgcc cgcccagctc tgcatcgcct
    16501 tcgacgagga gcacgcgacg gcccgcgcgc ccaagcgtcg cggccgccgt gagcccgttc
    16561 gcccccccgc cgatcacgac aacgtcgtac gtgccggcgc tcacgctctc gacctcaaga
    16621 tttccagggc tgcgagacgg cccgacgccc ccatgatgcc gcccccggga tgggttcccg
    16681 cgccgcactg ccagtagccc tggatcggcg tccggtactt cgcccactcc gggaccggcc
    16741 ggaggaagaa gagctgctgg agcgccagct cgccctggaa gatgttccct tcggagagcc
    16801 cggtgatccg ctcgatgtcg tcgggggtga gcacctggcg atgaaggatc agcgactcga
    16861 tgttgggagc atatcgcgcg agcgtcttca cgaccgcgtc gccgaatgcc tcgcgcctct
    16921 ggtcgttcca accgccgttc agcgtgtagg gtgcgtactg gacgaagatg ctcatgacgt
    16981 gcttcccggg aggagccatc cctgggtcga tcatcgatgg aatcacgatg tccatgtagg
    17041 gattccgcga gaactcgccg tacttcgcgt cgtcgtaggc ccgctcgagg tactcgatgc
    17101 tcgggctgat cgagatcgca ccgcggagat gggggccgtg ccctggcagg cacgtgaaat
    17161 ccggaaggcc cgagagcgcg aggttcacct tgcctgagga gccgcggaat ttgaagcggc
    17221 ggatcccctc gacgaggggg tcggggagct cgttcggatc aacgaggcgc gtgaacgtaa
    17281 ggcgcggatc aaggcctgag atcactgtgg acgccggaat ctcctcgccg ccggcgagga
    17341 caacgccgat ggcccggccc tcgcggacga gaacgtgctc cacagcggcg ctggttcgga
    17401 tctcggcgcc gtgcgcgcgg gccgcgccgg cgatcgcctc gctgatgccg ccggtcccac
    17461 ccttctggaa accccacgct cggaacgcgc cgtcgatctc gcccatgtag tggtggagga
    17521 gcacgtaggc cgacccgggc gagcgcggac ccaggaacgt cccgatgatc ccactcgccg
    17581 acttcgtggc cttgagcggg ccgaactcaa accactcgtc gaggtaatcg gcactgctca
    17641 tggtcatcag cttgtggagt gcgtggaagc gttcggggcc gagcccgcgg aaatgacgcc
    17701 cgagcttcag gagccccatg aggtccttcg gcgagagcga ggctgggtcc ggaggtacca
    17761 ttccaaggat cggtttcacc gccatcgcca tgtggtgcat gagccgcccg aaaaggatcg
    17821 tggcgtcggc gtcgcgcgtc gagtggcggc agagctcgcg gcgtgtctcg tcggggtcgg
    17881 cccacgatgc caggtagtcg ccgttgtcga gtggagtcac cgtgctctcg agcgggagga
    17941 tctgaagccc atggcgcggg agatccaggt cccggatgat ctccggccgg agcaggctca
    18001 ccacgtagga gaaaacggag aacttgaagc ccgggaagac ttgctccgta accgccgcgc
    18061 cccccagcac ggggcgccgc tcgagcacga gcgtgcgccg gcccgcgcgc gcgagatacg
    18121 ccgctgcgac gaggccgttg tgcccgcctc cgacgatgat cgcgtcgtag cgcggcgcgc
    18181 tcatgccttc ttccgcgggg gatcgtagaa gggaagcttc cgcaccacag cgtccacgcg
    18241 ctcacggtgg tgctccacgg tgacctcgag gctcaccgat gtgccggggg cgaaatgggg
    18301 ggcgcggagg tgcgcgagcg cgaggagctt cttgaggagc ggggaccagc agccgctcgt
    18361 ggcgtacccg acctgcctgc cgtccttgta gatcggcgcg ctcgcgcgcc acgcgatgct
    18421 cggaacctgg ggcgggaggc cgcgcgccgc gaaaagccgc tcgaagccca cccaatccac
    18481 ctcgagcccg acgaagcccc acgcggcacc ccgcgtgcgc tcggccgcga gcgctcggcg
    18541 gccgttgaac ggacccttgt ccgcgctcac ggcccagccg aggttgatct cgtagggcgt
    18601 ggacttccgc gcctcgatca gcgcgtggtg cgaggagaaa tagtccacgt ccagcatgat
    18661 gagccccgcc tcgatgcgcg caatgtcgag cgcccacacg cccgccggcg tgacgccgta
    18721 ggaagtcccc gcctcgacga gcgcatccca gagattcacg gcctgcgcag cgtccaccca
    18781 gatctcgtaa ccgaggtccc ccgtgtaacc ggtgcgcgag atcgcaaccg ggatcccgcg
    18841 cacggtggtc gggaccatgc ggaaatactt gagcgaccct agatcggcgg gggacgcctg
    18901 ggacagaacg gcgcgtgaga gcgggccctg gagggacatg gcccccatgc tctcggagat
    18961 gtcttcgatc cccacctcca tgcccgacgc gttcatgtga agccaccgga gcgacgaatc
    19021 cgcactcgtc aggcgaaact cgctcgatcc caggcgcgtc acggtgccgt cgtcgatgac
    19081 cttccccgcg tgatcgcacc agggggtgta gtagacctgg ccctcccgga gcttggtcat
    19141 gtcgcgcgta atcatgcggt ccagcaggcg ctcggcgtcc cggccggtaa tccggtactt
    19201 gtggagcgga gacacgtcga tcagcgcggc ggagttccgg atcgcggcgt attcccgatc
    19261 gggatgcgga tcgtaggcgc tcgccatctg gtagcccgcc cagcggcgcc acgtctgcgc
    19321 gcgcacaagg ggcgcggtgc ggggatgaaa gggagaagtc ttgagcatgg gaatcaggca
    19381 gtgtaagcga gcgctgcgag cgaaacaatc ggcatgtgtg cacacacgtg tgccgtcagc
    19441 agccgatttg ctccagaacc atcgtagggg aggcgatcac ggtcggcttg gcgagtaagt
    19501 cgcagatcga ttggtcgcga ggtgagagag attggccgcg agcgttccta aggcacgctc
    19561 caggagctgt ccgggtcgaa ggagttgatg gcctcggccc gcgcgagggt ttcggcgccc
    19621 aggtcctgct gccgaagctt ggcgtcgggt ccgatcagga acgtcatcac cccgctcttg
    19681 ccgtaatccg ccggccacgc gagcaagccg aatccaccgg tcatcctgct cccgacgatc
    19741 cacgagcgtt cgccgccgtc agcgctcgga ccctgtgcgg tcagcacctt gaagaagtac
    19801 ccctcgtagg gagcaggacc ctcctgccgt cggtagccct cggcggccgc cttggcggcg
    19861 agctctccca tgggactcgg tgcggggctc gcaggcgtgg ccggccagaa gagtccatcg
    19921 tgtcgtccgg gctggctcag catacgctgg gcgaatgcgc ccttgggcac accgtcatga
    19981 cctgtcgcgg catactcgtg ctgcgcttcc acgaacgctt cggcgacgtc catcacggcg
    20041 agctcattcc gcccgatgcg ccggtagagc atctcctcct tgcccgccgc ggtatcgaat
    20101 ctccagcggc ccttctcctc gaccagtggg atgggtagcg ggtaggactc gttccccacg
    20161 accaccgtcc gcgtcggtcc ttctccctcg agccaccagc gctcgatcat ggccgcgatc
    20221 acgacctggc ggtcccgcgc ggcctgaacc ggatcggccc gattcatgac atcggacgca
    20281 tccgggccga gaagggcgtg cagggctgtc gtgtctccct cggaaagggc atggaacatc
    20341 gcctcgaccg cggcctccgg cgtcgcaaac gtctgctgct ccgccggttt ctgcgaacag
    20401 ctcgacatcg ccacaccaag tccgagaatc gcgatcaccg agaggaggcg cacgggagcg
    20461 cgctccctgt cgaaacgccg cccgggggtc atcgtctccg acctccaccg cctccaccgc
    20521 tgcgaccgcc gccgcggctg ctcccgccgc ccttactcga ggccacggac gatcgcccgc
    20581 gatcgctcga ggcgcgcgtg ctggatccgt tcccatagcc gccgaaggcg tccgattccg
    20641 gtcgacctgg cttgtcgttg gtcgatggac gtccccgcgc ggtggagctt tcccgatcgg
    20701 tcttggcgcc gcctcggtcg ccagctttgg ccttgtcccg gctcgcatcg cggcgatcca
    20761 cgtctttgct gtcgacccgg ttgccagcat tggtccggtt gctgtttccg ttccggttca
    20821 cattggtgtt gcgcggcgtc gcgacgttgc ccctcgggcg atagttcggg acgggacgcg
    20881 cgtacgggta gcggggacga gcgggcacga cccagaccga gcggtggtag taggcgccgc
    20941 cgccgtgcca tccgtagccc caggcgcccc acccgtaata gtcgttgctc attgcggcgc
    21001 cgatcgcgat ccccagagtg aagccgacga gggccgcgac cgcggcatcg ttgctcgacg
    21061 agctcgacgc cgactgcgtg tagaccacgt tcggatcgta ctggggtacg taaacgacct
    21121 gcggattcgc cggctcgatg atgatcgtct gcgacccgcc ctcgctattg acctcgacct
    21181 tctgctgttc gttcgatttg agattgccct tctccttggc ttgggcccgg aggcgctgga
    21241 tcgacgtgtt gacctgatcc ttgctgctca ggtaggccgt gccgatctcc ttcgtccagt
    21301 ccatgttgtc ggccagcagc ttgacgacat cggggaagat ggcgagggag gcgaacgcgg
    21361 cgtcaaactt cgcgttcatc gccgcctgct gcagatcgct cccggtggcc gtctggtcct
    21421 tcagccactt gctgaactcc gtgacctggt ccgggctggt cgaagcggtg aggatctgcg
    21481 ccaggagcgc gtcgggatag agcgcgatgg gcgcgagcag cttgtcgagg ttgtccgcgt
    21541 cagaggccgg ggcggaggcg gggtagaggg cgaacgcgac gaggcatagc gcggcgagtg
    21601 cgacgagtgg gtgcgagcgc gagatcatgg ctctccctcc ctgggacgag gcacccgcgg
    21661 gtcatccgac agcggctgca tcccagccag ggtaggggcc gcgtccccag gttgccacca
    21721 ctaactagcg agcgatgcga tcgtcgatga gcagatcgcc agtcggtaag attccacccg
    21781 attcgtcccc agaatattcg gtgtaagctc tgctccgtcg gaggggcggc cgtgcggcct
    21841 gagagcgggg ctcatccctt ctgtcggtaa agtgcaagag agggtaagag ggcgtggcca
    21901 caatcggatc acagatatcc gttaggaggc cgggtgctgc ggacggtacg gtgcgtcttt
    21961 ctcgcggttc tgatccttgc ttcgaccttc ccggctcgga cggctctggc tcttggcgcg
    22021 caggaggtcg gaagcatcct gtcgattcgc cccctaggcg aggcggagcc gttaccggga
    22081 gacgacggcc tgacgcacgt cgcctatgag ctccatgtcg tcaacccgag ccggtttctg
    22141 gtttcgatcg atttactcga gatcctggat gcgggaaatg gccggacgtt gggtcgcctg
    22201 gccgggtcgg ccctttcggc catgacgatc atgggcggcg gtgagaaagg ccctgttttc
    22261 ggtccctcgc attccggtta catctacctg gatgtcgtac taccgaacga ggtgctcccg
    22321 agtgcgatcg accatcggtt cacgatctcg ctccaggtgc gcgcggcagc cgacgacgac
    22381 caccacggcg gtcccgtacc ggcaagcgta gggctcgatt cctcgctgac cttcaccggt
    22441 gcccgggtcg agatcgaccg caggcccccg gcggtgatcg cgccaccctt gcgcggtgac
    22501 cactggctgg tcgccaacgg ctgctgcgac gccgtcaatc cacatcgcgc agcggtcatc
    22561 gcgatcgacg gcgcgcttta cgtgccggaa cgctttgcca tcgacttcgt ccagctcgat
    22621 cgggatggga agctgttgac cggtcccgtg gacaagctca ccagttactc ctacttcggc
    22681 gcgcccgtgt tctcggtcgc gagcggtacc gtggctgctg cacatgatgg agagccggag
    22741 gaggtacccg gcgcgatgcc gaagggcaag acgatctcca ccgccacagg caactacgtc
    22801 atcgtggaca tcggtagccg gcgctatgcc gtctacgccc acttcaagac aggaagcgtt
    22861 cgtgtgcggg ccggtcagca cgtcgccgag ggggatgtcc tgggccttgt cggcaactcg
    22921 ggcaacacgt cgaatcctca ccttcatttc catgtctcgg accgggcctc gccgctttcc
    22981 tcggttgggc tcccgtatgt gttcaagcgg ttcgaggatc agggcatcgt gtccggtcta
    23041 gatgcagtca tcggaggagg agccggcacg atccaaaggg cgccgcagcc tggaccccat
    23101 cgcaaccagt tgccgctgga caatcacgtc gtcggcttcg gtcccacgaa ctagcggacc
    23161 atccgatagt cgaggagcag atagccggtc gtggaccaac gcagcttgcg attattgacc
    23221 ggcaacgcta cggcccgcaa acggaggtat ttgggcgtgc gtgagatcgt cctatgggtg
    23281 aggccatcct tggcgctgtc taacctgtta cgctcaaaga cgccctcgac gaaaaggcca
    23341 cgtttcccca accctaggga ggaacgacca tgacccgctt gaccacgcct tcctatcccc
    23401 gctggacccg aaccctgttg gttcttgcgc ttctgatcct cggcgccgag cccgcgagcg
    23461 cgcaacagag ccgcaatgtc acgctcctct cgcacatgga tctctattcc atggccggct
    23521 actcctcctg ctggtcgtac atccacagcg acgggcgcga gtacgccgtg gagttttcac
    23581 ggtcgggcgc ctcgatcgtg cgcctcgccg atccgcagaa cccagtggaa gtggcgttct
    23641 tcaatcttcc cgattccgaa tggcatgagg gacgtcaata ccaacactac ttctatatca
    23701 ccaccgagtt cgtgaacaac tacaaccccg gtgggctcac gatcctggac atggcggatc
    23761 cggagcatcc acggaggatc tccaacttcg actctccaat cctgtggtca cacaccatcg
    23821 aaatcgacac ggcccgtggt ttcctctata ccccgggggc ggccgggacc ggtccgtcgg
    23881 gcaacatcgt gcaaggtctc ttcatcttat ctctcgcgaa tcctgagaac ccgaccgtga
    23941 tcgccgtgta cggggacgag gcgcccaact acacccacac cattcacgtg cgcggaacgc
    24001 gcggatacgc ctccatgcag aatcagggca cggtccggat cttggatctg accaacccgg
    24061 cacagccctc actccttgcc gagattcaaa cgcccgctgg tgtggagcca ggcggcgatc
    24121 gaacactctt ccgccgcacg cacagtgcgt gggaatccgc ggacgaacgg ttcctctacg
    24181 tttccgatga gacgtcaccc cttggcgtct acgtgtacga catccaaaac ctcgccaaca
    24241 ttcagcgtgt gtacacattc gaggggatgc ctccgcgaag catcggccac gatcccgttg
    24301 ttcggggcaa ccgcttgttc ctctcgtact acacgactgg ggcgcgggtg tacgacatct
    24361 cgaatccggc gtggcccgtg gaaatcgggt actacgacac ctacgacggg cgtgacggcg
    24421 ggttccatgg atgctgggaa gtcgcgccgc tctttccctc ggggatcttc gtcgcgagcg
    24481 acatggagac tggtctctac gtcttccgcc tcgacgcgac gtacggaatc gttcgaggca
    24541 ccgtgcggaa ggggcccaat gggccgccgg tcgccggcgc cctcgtccgc caagcgggct
    24601 cgagcgcctc tacggtcagt ttctccgacg ggcggtacgc cttggccgta acgccgggct
    24661 cgaacgtgac gctgaacaca acgctcttcg gatatgaccc gctctcgaag tccctcgccg
    24721 tgaccgccgg aagcgatcgc ccgctcgacc ttccgttgcg tgcctcggat gcgggcagca
    24781 tctccggtgt cgttcgtgga acgccgggtt cgcccacgct caatgaggcg gagctcgaaa
    24841 tcaccggg
//
LOCUS       DASTKC010000054         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_40751, whole genome shotgun sequence.
ACCESSION   DASTKC010000054 DASTKC010000000
VERSION     DASTKC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40751"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFR25_02410"
     CDS             complement(<1..215)
                     /locus_tag="VFR25_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9939933.1"
                     /translation="MKPFVVAIDGPAGAGKSVTAKAVAERLGLNAVDSGAMYRAVGWH
                     ALRSGVPLDSEAALLGLLSRARIEAGP"
     gene            complement(212..1492)
                     /gene="aroA"
                     /locus_tag="VFR25_02415"
     CDS             complement(212..1492)
                     /gene="aroA"
                     /locus_tag="VFR25_02415"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9939934.1"
                     /translation="MSRATLRPSGPLRGACTVSGDKSITQRLILLGALARGTTRIEGG
                     NEGADARAALGIVKALGVPVRATGAGSWTIRGGALKESDRVLDARNSGTALRLSMGML
                     AAQPFFSVLTGDASLRRRPLSRVIEPLRALGADLSARDQDRLAPVAIRGRRLHGASVR
                     TPVASAQVKSALLLAAIQAEGDSMIEEAAPTRDHTERLLPRFGAPVTQENGRIAVRGP
                     ASLQGIEARVPGDFSAAAFLWAAAAIVPRSDVTVRGVGVNPTRRAFLDLLARAGAQVD
                     EVDRRDESDEPVADVRVRAHALEPIHVDPDEARGMIDELPLAAVLAAFAKGESVIRGA
                     EELRVKESDRIATVAAGLRAIGVPCEERPDGWVIQGTGAPRGGSVVSEGDHRIAMAFL
                     VAGLRAKDGVRVEGVEAAAVSDPGFLPRLKGLLR"
     gene            complement(1489..>2264)
                     /locus_tag="VFR25_02420"
     CDS             complement(1489..>2264)
                     /locus_tag="VFR25_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET9939935.1"
                     /translation="IALGLALRGASRVTGYDANPAKGAAALEANAVEAVLDDIDPASS
                     DLVALALPATQTPAMIQAIAPRLPAQSILMDLAPVMQPSVVAASSAGILQRFVPAHPV
                     PSAPESTDPGRFRGATVLIGASTDPGSPGATVADLWSSIGAVPAPVAPTIHDALFALT
                     HDLPVLSAVALVRTLRRTGSLTRLLAPASRAMLDAATRAAGAGSSTDTLAINAPKLLP
                     ALELLEREVRRLRHALEEGGPELGHLLDEARQFRQELVR"
BASE COUNT          324 a          818 c          829 g          293 t
ORIGIN      
        1 tgagggccgg cctcgatccg cgcgcgcgac aggaggccga ggagcgcggc ttcggagtcg
       61 agagggacgc ccgatcggag cgcgtgccac cccacggcgc ggtacatcgc gcccgaatcc
      121 acggcgttga gcccaaggcg ctccgccacg gccttggcgg tgacgctctt ccccgctccc
      181 gcgggaccgt cgatcgcgac cacgaagggc ttcatcgaag gagacccttc aatcgcggga
      241 gaaagcccgg gtcggacacg gccgccgctt cgacgccctc gacgcgcacg ccgtccttgg
      301 cgcgaagccc ggcgacgagg aacgccatcg cgatccggtg gtccccctcc gagacgacgg
      361 atccgcctcg tggagcgccg gtcccctgga tcacccagcc gtcgggccgc tcctcgcaag
      421 ggacgccgat cgcgcggagt cccgccgcca cggtcgcgat ccggtccgat tccttcacgc
      481 gaagctcctc ggcgccgcgg atgacgctct cccccttcgc gaacgcggcg agcacggccg
      541 cgagcggaag ctcgtcgatc atcccgcgcg cctcgtcggg gtcgacgtgg atcggttcga
      601 gcgcatgggc tcgcacgcgc acgtcggcca ccggctcgtc cgactcgtct ctccggtcga
      661 cctcgtccac ctgggctccc gcacgcgcga gaagatcgag gaacgcgcgg cgcgtgggat
      721 tcactccgac gccgcgcacg gtcacgtccg atcgcggaac gatcgcggcg gccgcccaca
      781 ggaacgccgc ggcgctgaaa tcgccgggaa cgcgggcttc gatcccctgg agcgaggccg
      841 gccctcggac ggcgatgcgg ccgttctcct gcgtgacggg tgcgccgaat cgcgggagca
      901 ggcgctccgt gtggtcccgc gttggagccg cctcctcgat catcgaatcc ccctcggcct
      961 ggatcgccgc gaggaggagc gcgctcttca cctgggcgct cgcgaccggc gtgcgcaccg
     1021 acgcgccgtg gagccgtcgt ccgcgaattg cgaccggcgc gaggcggtcc tggtcgcgcg
     1081 cggagagatc cgcgccgagc gcgcgcaacg gctcgatcac ccgggagagc ggacgccgcc
     1141 tgagcgacgc gtcccccgtg agcacggaga agaagggctg cgccgcgagc atccccatgg
     1201 agagccgcag cgccgtgccc gagttcctgg cgtcgagcac gcggtcgctc tccttgagcg
     1261 cgccgccgcg gatggtccac gagcccgcgc cggtcgcgcg caccggcacg cccagcgcct
     1321 tcacgattcc gagcgccgcc cgcgcgtcgg cgccttcgtt tcccccctcg atgcgggtcg
     1381 ttccccgcgc gagcgcgccg agcaggatga ggcgctgggt gatcgacttg tctcccgaga
     1441 cggtgcaggc gccccggagc ggcccggacg gacgcagcgt cgcccggctc aacgcacgag
     1501 ctcctggcgg aactgacggg cctcgtcgag gagatgcccg agctcgggtc ctccctcctc
     1561 gagcgcgtgc ctcagccggc gcacttcccg ctcgaggagc tcgagcgccg ggaggagctt
     1621 cggcgcgttg atcgcgagcg tgtcggtgga cgagcccgct ccggccgcac gcgtcgcggc
     1681 gtcgagcatc gcgcgggacg ccggcgcgag gagacgcgtg aggcttccgg tccgtcgaag
     1741 cgtgcgcacg agcgcgaccg ccgagaggac ggggagatcg tgcgtgagcg cgaagagcgc
     1801 gtcgtggatc gtgggagcca ccggtgcggg caccgctccg atcgacgacc agaggtcggc
     1861 gaccgtcgcg ccgggcgagc ctggatcggt cgatgcgccg atcagaaccg ttgcgccacg
     1921 aaaacgccca gggtcggtcg attccggtgc ggacggaacg gggtgtgcgg gcacgaagcg
     1981 ctgtaggatg cccgcgctcg aggcggcgac cacggacggc tgcatgacgg gtgcgagatc
     2041 catgaggatg gactgcgctg gaaggcgtgg agcgatcgcc tggatcatcg ccggcgtctg
     2101 ggtggccgga agcgcgaggg cgaccagatc gctcgaggct ggatcgatgt cgtcgagaac
     2161 cgcttcgacg gcgttcgcct cgagcgccgc ggcgcctttc gcaggattgg catcgtagcc
     2221 cgtcacccgc gaagccccgc ggagcgccag cccgagagcg atgc
//
LOCUS       DASTKC010000055         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1696111, whole genome shotgun sequence.
ACCESSION   DASTKC010000055 DASTKC010000000
VERSION     DASTKC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696111"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..2031
                     /locus_tag="VFR25_02425"
     CDS             91..2031
                     /locus_tag="VFR25_02425"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9939936.1"
                     /translation="MRCASSSFARLRRALGAAFLAVVALGTAPAGAAVSASTSAEGFS
                     REALRTLARPQGVRVIEGVSGDPRETVQALERAGYHVDVAVLPSTFFVRERGPHRALP
                     AGFLDVTGPAEDPATPMSSAPSYSGDSDVNPFQGRADMLPPLDRTGIDGPGLGPNAAP
                     PMPAGLPHGARWTDTSEFMMGRVAVSLIFPESDGSIDANLYDWTPALRDSVARSAVRG
                     LLKWTTFAAAHDVPLTFLIEVHASVPTGYEPITRTTSFELDWIREALKPIAGYGTDAE
                     KEAYDVVNAARARLGAHWGTLILAVQNDTDPDGAFPDGFIAHANLGGPWLVLPINNAN
                     TRSAALDYYVEHEMTHQFWALDEYASNSAWWSCTLLTGYFGWPNSNSVIPAPRYCGQD
                     DRCMMKGNYPDSLCSSTMGSIGWVDRDHSGVLDFYETRPSVRTDSTHYGNMPGFPIHV
                     TGTAGDVAFPNTNPFLFGAGDSITIATIDSLWYRVDGGPWTTLPSDDGVMDEGAERFS
                     LTFNLPVADHVIHFRARNSSGREQLVPDSVIVTVSGSSGSPAPGGGAPRPSVSAAPTP
                     SRGPVHLGVRAPRGAVTKAEIFDARGRRVRAWEFRIPDTGLMTWDWNGAADNGRTLSS
                     GVYYLRVRWKDGEIARRVVLLR"
     gene            2045..2599
                     /locus_tag="VFR25_02430"
     CDS             2045..2599
                     /locus_tag="VFR25_02430"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HET9939937.1"
                     /translation="MTSLERLATQTPAEAPAAPLRGRRYDEDRLHLLVRDPHRAAAIW
                     EISSKLHARAESMAKAARAPVRYRIVVHQAPSPEGAPIATIAIDLPDALRDETWFFDL
                     QVSGGVARAELGLDLPSGLAVLLTSRWMPVPPDGPCAETGAWSVDPASAAWLDAEAAR
                     ARSGSPRTSPSSVARYLATRPGES"
     gene            2596..>3547
                     /locus_tag="VFR25_02435"
     CDS             2596..>3547
                     /locus_tag="VFR25_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HET9939938.1"
                     /translation="MTTSGPSGFLSILLHAHLPYVRHPEYEDPLEERWLVEALLECYL
                     PLLGVLERRADRGARGRITLSLSPTLIAMLDDDLIRGRLAHRLARLEDLAARDVRRHA
                     NEPGLERLSRHYLDRFREGRRRYEDTYRRDVIEAFASLEKAGVVSLVTCGATHGFLPL
                     MDMGAPSWRAQIGTAARELERRLGHGPEGMWLPECGYTPGVESVLAEFGVGWFVVDAH
                     GIANACPRPLPGTYAPFRTGAGVLALGRDVDASRQVWSATEGYPGDPEYREYYRDQGF
                     DLPLDEVRSDLPSGGTRTHLGLKYHRITDRRFEHRELYDPD"
BASE COUNT          512 a         1262 c         1224 g          549 t
ORIGIN      
        1 ccttgatccg ctgggacggc agggacgcga ggtcgaatca ggcgggaagc ggcgtgtact
       61 ggatcgttgc ggaatcggga ggagagcgcc gtgcggtgcg cgtcgtccag cttcgctaga
      121 ttgagacgcg cgctcggggc cgcgttcctc gcggtggtgg cgctcgggac cgctcccgcg
      181 ggcgcggccg tgagcgcgtc cacgtccgcg gagggcttca gccgcgaagc gctccgcacg
      241 ctcgcgcggc cgcaaggcgt tcgcgtgatc gaaggcgtga gcggcgatcc tcgggagacc
      301 gtccaagcgc tcgagcgcgc gggctatcac gtcgacgtcg ccgtcttgcc gtccacgttc
      361 ttcgttcgcg agcgcggccc gcaccgcgca ttgcccgccg gcttcctgga tgtgaccggg
      421 ccggccgagg atccggcgac accaatgtcc agcgcgccgt cctactcggg ggattccgac
      481 gtcaatcctt tccagggccg ggccgacatg ctgccgccgc tcgaccgaac cgggatcgac
      541 gggccagggc tagggccgaa cgccgcgccg ccgatgccgg ccggtctccc gcacggagcc
      601 cggtggacgg atacgagtga attcatgatg ggacgcgtcg cggtctcgct catcttcccg
      661 gaatccgacg gcagcatcga cgcgaacctc tatgactgga cgcccgcgct ccgcgactcg
      721 gtcgctcgct ccgcggtgcg gggcctgctc aagtggacca cgttcgccgc ggcccacgac
      781 gtcccgctca cgttcctgat cgaggtgcac gcatcggtcc cgacgggtta cgagccgatc
      841 acgcggacca cgagcttcga gctcgattgg atccgggagg cgctcaagcc gatcgcgggg
      901 tacggcaccg acgcggagaa ggaggcctac gacgtcgtca acgcggcgcg cgcgcggctc
      961 ggcgcacact gggggacgct catcctcgcg gtgcagaacg acaccgaccc cgacggtgcg
     1021 ttcccggatg ggttcatcgc ccacgcgaat ctgggcgggc catggctcgt cttgcccatc
     1081 aacaacgcga acacccggag cgcggcgctc gactactacg tggaacacga gatgacgcac
     1141 cagttctggg cgctcgacga gtacgcctcg aacagcgcct ggtggtcgtg cacgctcttg
     1201 accggatact tcggctggcc caactccaat tcggtgatcc cggcgccgcg ctactgcgga
     1261 caggacgacc gctgcatgat gaagggaaac tatcccgaca gcctctgctc ctcgaccatg
     1321 ggctcgatcg ggtgggtcga ccgggatcac tccggcgtcc tcgacttcta cgagacccgg
     1381 ccctcggtcc ggaccgactc gacccactac ggcaacatgc ctgggttccc gatccacgtc
     1441 accggcaccg ccggagacgt cgcgttcccg aacacgaatc cattcctgtt cggggcaggg
     1501 gattcgatta ccatcgctac gatcgactcg ctctggtacc gggtcgacgg cggcccctgg
     1561 accacgctcc cgtcggacga cggcgtgatg gatgaagggg cggagcgctt ctcgctcacg
     1621 ttcaacctgc cggtggcgga ccacgtgatc cacttccggg cgcgcaactc gagtggccgg
     1681 gagcagctcg ttccggacag cgtcatcgtc acggtgtcgg ggtcgtccgg ttcccccgcg
     1741 ccgggcgggg gagcgccgag gccttcggtg agcgccgcgc cgacgccgag ccgcgggccg
     1801 gttcacctgg gcgtgcgcgc gccccgaggg gcggtcacga aggcggagat cttcgacgcc
     1861 cgcgggcggc gcgtccgcgc ctgggagttc cggattccgg acacgggtct catgacgtgg
     1921 gactggaacg gagccgccga caacgggcgg acgctctcga gcggcgtcta ctatctgcgc
     1981 gtacgatgga aggacggcga gatcgcgcgc cgcgtggtcc tgctccgctg acgaaaggga
     2041 atccatgacg tcgctcgaac gcctcgcgac ccagactccg gccgaggcgc cggccgctcc
     2101 gcttcgtggg cggcgctacg acgaggaccg gctgcatctc ctcgtccgcg acccgcaccg
     2161 cgcggccgcg atctgggaaa tctcgtccaa gctccacgcg cgcgcggagt cgatggcgaa
     2221 ggcggcgcga gcgccggtcc gataccgcat cgtcgtgcat caagcaccga gtccggaggg
     2281 ggctccgatc gcgacgattg cgatcgatct tcccgacgcg cttcgggacg agacctggtt
     2341 cttcgatctc caagtgtccg gcggcgtcgc ccgtgccgag ctgggactcg acttgccgtc
     2401 gggactggcc gtactgctca cgtcgcgctg gatgccggtg ccgccggacg ggccctgcgc
     2461 cgagacaggc gcctggtccg tcgaccccgc gtcggccgcg tggctcgacg ccgaggccgc
     2521 gcgcgcccgg tccggatccc cgcgcacatc tccgagctcc gtcgcgcgct atctcgcgac
     2581 ccgccccggc gaatcgtgac cacatcgggc ccgtcgggat tcctttcgat cctgctccac
     2641 gcgcatcttc cgtacgtgcg ccatcccgag tacgaggatc cgctcgaaga gcgctggctg
     2701 gtcgaggcgc tgctcgagtg ctatctcccg ctcctcggcg tgctcgagcg gcgtgccgac
     2761 cgtggcgcgc gggggcggat caccctctcc ctctcgccca ccttgatcgc gatgctggac
     2821 gacgatctga tccgggggcg cctcgcgcac cggctcgcgc gcctcgagga cctggccgcg
     2881 cgcgacgtcc gccgccacgc gaacgagccc gggctcgagc ggctctcacg ccactacctc
     2941 gaccgcttcc gcgaggggcg gcggcgctac gaggacacat accggcgcga tgtcatcgag
     3001 gcgttcgcgt cgctggagaa ggcgggcgtc gtgagcctcg tcacctgtgg cgccacgcac
     3061 ggcttccttc ctctgatgga catgggcgcg ccctcatggc gcgcgcagat cggcaccgcc
     3121 gcacgggagc tcgagcgccg tctaggccat ggacccgagg gcatgtggct cccggaatgc
     3181 ggctacacgc cgggcgtgga gtcggtcctc gcggagtttg gagtcgggtg gttcgtggtg
     3241 gatgcgcacg ggatcgcgaa cgcgtgcccc cgtccgctcc cgggaaccta cgcgccgttt
     3301 cgaacgggcg ccggcgtgct cgcactgggc cgtgacgtgg acgcgtcgcg tcaggtgtgg
     3361 agcgcgaccg agggctaccc cggcgatccc gagtaccgcg agtactaccg cgaccaggga
     3421 ttcgatctgc cgctcgacga ggtccgctcc gatcttccgt cgggcggcac gcggacccac
     3481 ctcggactca agtatcaccg gatcacggac cgccgcttcg agcaccgcga gctctacgat
     3541 cccgatc
//
LOCUS       DASTKC010000056         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_986683, whole genome shotgun sequence.
ACCESSION   DASTKC010000056 DASTKC010000000
VERSION     DASTKC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986683"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFR25_02440"
     CDS             complement(<1..373)
                     /locus_tag="VFR25_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9939939.1"
                     /translation="MEDPKRILVTRIRQIGDVILTLPILDALRARFPDATIDYLAEGA
                     PAQAAIGHPALRRVLTLRKWPGLPGAPHLLAALRRAQYDWVIDLYGNPRSAFLTRVTG
                     APVRIGPARRSRRQHYTHAIPA"
     gene            complement(375..854)
                     /locus_tag="VFR25_02445"
     CDS             complement(375..854)
                     /locus_tag="VFR25_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET9939940.1"
                     /translation="MSRAKMRDREELARALDRDPALRRGLVLANGLFDLIHVGHVRYL
                     EGARACGERLLVAVNSDDSARRLKGPDRPYVPLAERMEIVAALDCVDWVIGFDEPNVE
                     TLLRALRPAVHAKGTDYTVESVPERAVARELGIETVVVGDSKDHATSDLARRVREGR"
     gene            complement(851..1927)
                     /locus_tag="VFR25_02450"
     CDS             complement(851..1927)
                     /locus_tag="VFR25_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9939941.1"
                     /translation="MSPPRARTARASREAMSAAPDRDRLLELSAALRDVPVAVVGDLV
                     LDRYWFGRSSRISREAPVLILDFEEERGVPGGAANAAMNARSLGGRPRLVGRVGRDEA
                     GRDLARSLRAEGLDPRWLLRDNGAGTIVKTRILAGSSHTSRQQIVRLDRGHGALMPRS
                     GERSLERALARAMKGSSAALLSDYGYGVLAPPLRRAAIAAARRAGLPVVVDSRYALRS
                     YRGVTLLTPNEHEIRDAFHLPSLDTGALRRAGEDLRERTAARGVWITRGSDGMMLLER
                     GRKALRIPIVGSSDVADVTGAGDAVSAAATLALGAGASLVEAALLATYAASVVVMKRG
                     TATVSRAELDAAIRAHPVPRPEPA"
     gene            complement(1924..>2320)
                     /locus_tag="VFR25_02455"
     CDS             complement(1924..>2320)
                     /locus_tag="VFR25_02455"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217682.1"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET9939942.1"
                     /translation="AQLSCPDMRLPLLYALSFPERWTSSLPRLDLAKLGSLAFEAPDP
                     ARSPGLALARRALERGGTTPAVLNAADEEAVQLFLTGVIGFSDLTRLVGEVLDAHAPT
                     GPLTLESIADADRWARARLADLAASRARR"
BASE COUNT          341 a          832 c          849 g          298 t
ORIGIN      
        1 gggctgggat cgcgtgcgtg tagtgctgtc gtcgagaccg ccgtgccggt ccaatgcgaa
       61 ccggcgcgcc ggtgacgcgc gttaggaaag cgcttcgggg attgccgtag agatcgatca
      121 cccagtcata ctgtgctcgc cgcaacgcgg cgagcagatg gggtgctccc ggaaggccgg
      181 gccactttcg aagcgtgagc acccttcgga gcgcgggatg tccgatggcc gcctgcgcgg
      241 gcgctccctc ggccaagtag tcgatggtcg cgtcgggaaa gcgcgctcgg agggcatcga
      301 ggatcggaag cgtgaggatc acgtccccga tctgccggat ccgcgtgacc aggatccgct
      361 tcggatcctc cacgtcagcg cccctcgcga acgcggcgcg cgagatcgct cgtcgcgtga
      421 tccttggagt cgcccacgac gacggtctcg atccccagct cgcgcgccac ggcgcgctcc
      481 gggacggact ccacggtgta gtcggtcccc ttcgcgtgaa cggccggccg gagggcgcga
      541 agaagcgtct ccacgttcgg ctcgtcgaat ccaatcaccc agtcgacgca gtcgagcgcc
      601 gcgacgatct ccatgcgctc ggcgagcgga acgtagggcc gatccggccc cttgaggcgg
      661 cgcgccgaat catccgaatt cacggcgacg aggagcctct caccgcaagc tctcgccccc
      721 tccaggtagc gcacgtgccc cacgtggatg agatcgaaga ggccgttggc gaggacgagg
      781 ccgcggcgga gcgctggatc ccggtcgagt gccctcgcca gctcctcccg gtcgcgcatc
      841 ttggcgcggc tcacgccggc tccgggcgtg gaaccgggtg cgcgcggatc gcggcgtcca
      901 gctcggcgcg cgacaccgtc gctgtgcccc gcttcatcac gacgaccgac gcggcgtacg
      961 tcgcgaggag cgcggcctcg accagcgagg cccctgcacc tagcgcgagt gtggcggccg
     1021 cggagaccgc atcaccggcc cccgtcacgt cggccacatc cgaggatccg acgatcggga
     1081 tgcggagcgc cttccggccc cgctcgagca gcatcatgcc gtcgctcccg cgcgtgatcc
     1141 agacgccgcg cgccgcggtg cgctcgcgga gatcctcccc ggcgcggcgc agcgctcccg
     1201 tatcgagcga cggaaggtga aacgcgtccc ggatctcgtg ctcgttcggc gtgagcaggg
     1261 tcacgccgcg ataggagcgg agcgcgtacc gggagtccac caccaccgga agtcccgcgc
     1321 gccgggccgc cgcgatcgcg gcgcgtcgaa gaggaggcgc gagcacgcca tagccgtaat
     1381 cggagaggag cgccgcgctc gagcctttca tcgcccgcgc gagcgcgcgc tcgagcgacc
     1441 gctcgccgga gcgcggcatc agggctccgt gtccgcgatc cagccgcacg atctgctggc
     1501 gcgacgtgtg gctggatccc gcgagaatgc gggtcttcac gatcgtgccc gcgccgttgt
     1561 cccggaggag ccaccgcggg tcgagcccct cggcgcggag cgagcgcgcg aggtcccgcc
     1621 cggcctcgtc ccggccgacg cgccccacga gcctcggacg gcccccgagc gatcgggcgt
     1681 tcatggcggc gttcgccgcc cctccgggca cgccgcgctc ctcctcgaag tcgaggatga
     1741 ggacgggagc ctcgcgcgag atgcgggacg agcggccgaa ccagtagcgg tcgagcacga
     1801 gatcgccgac caccgcgacc ggcacgtcgc gaagcgcggc gctcagctcg aggagccgat
     1861 cgcgatccgg ggccgcgctc atggcctcgc ggctcgcgcg cgcggtccgc gctctcggcg
     1921 ggctcatcgt cgcgcccgtg aggcggccag atcggcgagc cgggcgcgcg cccaacgatc
     1981 cgcgtccgcg atcgattcga gcgtgagcgg ccccgtaggc gcgtgcgcgt ccaggacctc
     2041 gcccacgagg cgggtgagat cggagaagcc gatcacaccg gtcaggaaga gctgcaccgc
     2101 ctcctcgtcc gcggcgttca ggacggcggg ggtggtgcct ccgcgttcga gcgcccggcg
     2161 cgcgagcgcg agcccgggcg agcgcgcggg atcgggagcc tcgaacgcga gcgacccgag
     2221 cttcgcgaga tcgagccgcg gcagggagga cgtccatcgc tccgggaagc tgagcgcgta
     2281 gaggagcgga agccgcatgt cggggcagga gagctgcgcg
//
LOCUS       DASTKC010000057        16282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1697275, whole genome shotgun sequence.
ACCESSION   DASTKC010000057 DASTKC010000000
VERSION     DASTKC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16282
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697275"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFR25_02460"
     CDS             <1..224
                     /locus_tag="VFR25_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939943.1"
                     /translation="ETRYTADIEGLPVGDAATRLTKAGMPAEDAAQWKESLERLEQLA
                     YAPPDARGAGGTALQSAAELVRRFREGLA"
     gene            221..913
                     /locus_tag="VFR25_02465"
     CDS             221..913
                     /locus_tag="VFR25_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939944.1"
                     /translation="MRLALFIVTATLVVAAEGHAATAPPPRADVLYNAGTAALDKGDV
                     GPAVAFLTAAARLEPRARDIQINLKQATVAAARARGVESQDEIEVPREVPLSTAEIAW
                     VGAILLLVGAALGIASRVTRLPRTLGIAAVLVVAVGAAIHVWLVARAHAEAVHPEAVV
                     VVPQLAVERGPDEPSRAPVVLGAGERVHLGPTRGGQVEIRIAGAAIGWAPHEGLWRVQ
                     ETPRYTARYATP"
     gene            926..1645
                     /locus_tag="VFR25_02470"
     CDS             926..1645
                     /locus_tag="VFR25_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9939945.1"
                     /translation="MSSASNTALVTGGTGGLGRAVVARFLADGLKVAVPYRSEAEWTA
                     LSSAHPDSVRSGELHGFVADVTDESSMADAASRAASAMGGLRVLAHIAGGYRGGVDVE
                     SVDANDVRSMIEVNLLSAFWAAKHVIPHAKRTGSGRLLFVSSRGAIQYYPGAAAYAAA
                     KIGLHGLVGTLAKELLADGVTANAVLPSTIDTAANRSAMPKGKFDDWVRPEQVAALLA
                     FLASDAASAVSGALIPIYGRA"
     gene            1645..2766
                     /gene="speY"
                     /locus_tag="VFR25_02475"
     CDS             1645..2766
                     /gene="speY"
                     /locus_tag="VFR25_02475"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HET9939946.1"
                     /translation="MAPGQKVSKGPKIAPDPIRPGITVDQLVDQSFLAYNGARLREGC
                     RLFVEKMLEPDVTVGITLSGALTPAGLGRSCLIPLLKAGFVDWIISTGANLYHDTHFG
                     IGLTMHQGRSNVDDVALREQGIVRIFDIFFDYDVLLSTDSFFREVMRQPEFRAPMGTA
                     EFHYRVGKYILEREKALGQKETSLLAQAHALEVPVYTSSPGDSSIGMNAAELWLDGAA
                     MHWDVFRDVNETAAIVYDAKQKGGKSAILILGGGSPKNFALQTEPQIQEVLGIEEEGH
                     DYFLQVTDARVDTGGLSGATPGEAVSWGKIDPDRLPDAVVAYVDSTVALPILAAYALS
                     KHEARPLKRLYKDRERLLDQLRAAHKGARAEAEPRGARS"
     gene            2763..3917
                     /locus_tag="VFR25_02480"
     CDS             2763..3917
                     /locus_tag="VFR25_02480"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9939947.1"
                     /translation="MKTVPTGTASTATPVPLLDLGLAHRAIAEELKRDFERVLTSGQF
                     ILGAEHDAFERELAQAAGLTHAIGLSSGTSAITLALQALGVGPGDEVILPAFTYFATA
                     SAVAHAGATPVFADVEPHRFGLDWASVESRLGPRTKAILAVHLYGLACDLAPLTRLAQ
                     TRAIPLLEDAAQAIGATDQGVPVGKADAGATLSFFPTKNLGALGDAGAFVTDRDDVAW
                     RVRLLSRQGDAGDYRHTVVGTNARLDALQAAFLRTKLRHLEAWVTARRDAAQAYREAL
                     AGVPVTLPEEPAGAKHTYHQFTIRAPRRDALQSYLREQGVATRIYYPIPLHIQPAFAH
                     LGLGPGTFPVSEQLAGEVISLPLFPGITREQIERVASCVRAFYTERPSRG"
     gene            3921..6461
                     /locus_tag="VFR25_02485"
     CDS             3921..6461
                     /locus_tag="VFR25_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET9939948.1"
                     /translation="MADTPKPAAAPRGKRILWIVLGSIAGLVVLVYLAAWLLLPKDFV
                     ESQAMKMASKNQGATIRWTRMTTGLQGFSFGVKIEGMTIRMPAEGKGEPSLDGRIGEV
                     IVRFRLLPLLFRKVEIDAAQVNGAGIAMYERGKPPGEGKKKEEKPSFALHLPKLGFDR
                     VDVRSRDRYGSGYDLRGLSGSVAIDGNLDAFRSAKIEAKGDSVSWKPSAQGKAVPLPG
                     PLHATATLESRDQGKRYEFTQGHIEVGALTSELSGSFRKPAEGSDAMPLAIVITGKPQ
                     ALKSSDAAFRALMPESPGSTPVDWKTTASWKIDIGGTSEAMVQNGEANLRPLSIEAGP
                     NKFTLDRVQVRYETTTDQKFTMDATGAGSGVALAVAAAGSTAPGGGSKGDLHVSAPAT
                     RLNGLMPNTPVWTMGTLEVGATFALEPPAAPVVRWTVTGKDLSGSVTGLQRPVTDLQF
                     HIDGDQQTANVRSMTARVGSSNVSLTGAVKQGKPLGTGTFRASMDKFVAEEWADPKTA
                     QEKGKGKDSKAPAPPPAIPLRSFDGLVTIGEVHSGNLTIRNLSAPIAFASDKLVAAPI
                     QGDMGTGTLAGKVEVANITKDPSYAVHFDLKRVPVREFVGGMMPLNLGLTGFATGQAD
                     ISGAGLPGLAAMNTLTGALQGNVEEGKILETPTINALRSALGTVNGPGSLGDLVFKTL
                     QYSAKIDKGRLVLNQVNGALGKDLMQLAGSAGFDKSLDLDLLLKLAPGRIQSSTALGQ
                     FANYARDEQGRLPIKVAITGTVTNPKVSLKAASTVEAAGKGLAKEIFENIMRGNRPDT
                     TRADTSKAHADTSRADTTRAAKKPARADTTVSEPLKKAQEALEKIFKK"
     gene            6458..7474
                     /locus_tag="VFR25_02490"
     CDS             6458..7474
                     /locus_tag="VFR25_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9939949.1"
                     /translation="MTQRGPAPAIETHRLTRLFKARRRQAEVHALEGVDLRVERGECF
                     GLLGPNGAGKTTLIKILTTLLLPTTGTATVAGFDVVNDPLQVRRRINMVSGGDTSGYG
                     ILTVRETLHLFARLYGVPHAEARRRADALMDVTGLGPKADALLSSLSTGMKQKLNFAR
                     AFMSDPEIVFLDEPTLGLDVEAARDIRAFVTRWIEERPDRTVLLTTHYMVEADELCGR
                     VAIIDRGQILALDTPRALKRAISSEPVFELQIGTGTAQFEDLRDVYGVRSLSHHAHPA
                     TGAIELRVAVVEDQVIGEVLGRLKERGASVLNLTKSEPTLETVFVHLVGRGLEDEDTD
                     GQGA"
     gene            7476..8306
                     /locus_tag="VFR25_02495"
     CDS             7476..8306
                     /locus_tag="VFR25_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9939950.1"
                     /translation="MLPSDWRERLRLNWGAAQARARVRIVASWREKSWIIGETVFPFL
                     AMSAFILVYRGLHAPKIYEAFVVLGGAMIAYWNNVLWSMASQFFWEKEQGQLQLYLMA
                     PVSRMSILLGMALGGMIMTTSRAIVILTGGILIYHVPLPLDRAFPAFLMFLLTLGAVY
                     SLGMILSSLFLLWGREVWHTATLFQEPIHLLSGFYFPVRALGAVAAGLASLLPITLGL
                     DGIRQILYGKAAHGLLPLAWIPPLQILLLAFFLFLAHKSLALMENLGKREGRLTLRGG
                     "
     gene            8303..9175
                     /locus_tag="VFR25_02500"
     CDS             8303..9175
                     /locus_tag="VFR25_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9939951.1"
                     /translation="MSPPAERPGALRRQADVIGASAWLGWQIDANWADPFTFLVFSIA
                     KPLATSLILYFMVKIVSPASATGEAFLFLFLGNTFFLYVTEVLIGISWTVFRDREDYE
                     TLKYIYLAPMPLRSYLFGRGMAKMGTATLGVIVALLFGRFVLHLPIGTPHTPWGLVIL
                     AVLLGLVAVAWLGIVLAGVSLVVARHSINLNEGLSGVFYLLSGAVFPIEVLPGFAQRI
                     SLWLPFTYWLELLRRLILGKSFSKVLQGVSDGRLWLVLVLSTVGIAIFAWVWFRFCEH
                     VARSRGLIDWKTNY"
     gene            complement(9197..9697)
                     /locus_tag="VFR25_02505"
     CDS             complement(9197..9697)
                     /locus_tag="VFR25_02505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9939952.1"
                     /translation="MISPKQRRQKQLAVVVAIGREHFLTGVRMMYACRYGDALVHFQE
                     AVMLEPNSKGYLSYLGVAVAHAERKYADAEQLCRRAIEAEYHRPEHYYNLGEVHFMAG
                     RKSDAIKCFNQALSWNPNFEAAQDALRKLGIRKAPVLPMLSRSHPVNVILGKALRKKV
                     RRPLPA"
     gene            9962..10459
                     /locus_tag="VFR25_02510"
     CDS             9962..10459
                     /locus_tag="VFR25_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9939953.1"
                     /translation="MTSPSLAASKNASSLESLLRPVHFVSELRSKKKAQVLDELAAAL
                     AATGVTRSPEIVAELLREREALGSTGVGKGIAVPHARSTLIAERALLVARSQKGIEFD
                     AVDGEPVHLLFLVVAPPTERDPIYLKVLADVVRAVRLSRTRQRLGDAPDFETVREILI
                     HAAAE"
     gene            10443..10799
                     /locus_tag="VFR25_02515"
     CDS             10443..10799
                     /locus_tag="VFR25_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET9939954.1"
                     /translation="MPPLNEQLDALVTLQDIDLMLRDSRDPEMGTHEDRLGFARQNVK
                     GLEKTRERLRSQIDEPLLRTYERMSRRIPRLVVPVEGRVCQGCRMSLPTSSAGRNTPG
                     TTIETCENCGRILYRI"
     gene            10879..11295
                     /locus_tag="VFR25_02520"
     CDS             10879..11295
                     /locus_tag="VFR25_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939955.1"
                     /translation="MLKKTLASALAIMALGIVLTCLARSEPPPPTVVGPTQILYADAD
                     TVYFLRSREWWSGNVRHQEKLLLHGLKMKYWRDRLPDDMRLVFDELGYPTGRVLLTPY
                     GHTEEHWYYGPAGPPLRFRDGVLLDQDRFDALRSEP"
     gene            11417..11986
                     /locus_tag="VFR25_02525"
     CDS             11417..11986
                     /locus_tag="VFR25_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939956.1"
                     /translation="MATVERPNNSLRILFARAAVLVIAAATLLALTGARPADAPATAP
                     LDQIAQAPVDQPFELPDTTEDEEDDADTLDVVPPSAQQQAPPPASPAVEDTTGAFRPD
                     LNTVPPGATADSSATGAAPDTLFMPSGVLPTNPQPTTTTTTQPQATPPLPGPKQEVKK
                     PRKGLLGIHPVAILFGLAALHIMVTHLAE"
     gene            11986..12678
                     /locus_tag="VFR25_02530"
     CDS             11986..12678
                     /locus_tag="VFR25_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939957.1"
                     /translation="MPRRGTGDGSGVALRVRWNPPDFALREPAERVLADAVRELGLAG
                     VIHDLHVFIDSANRDDHAYIEWNTHDHRAARLWFALGNFVTPKRRRMWSRTWARRSGT
                     PPLMARQFSARSFAEACLHELCHLKDDHESGVDLSGHPESDREALNELWNVWIDGRLN
                     RRGLPAMSRGERRRVFARTLASTPRYSAVGERVFRALWRADHLGPRELRAYLEELKGP
                     RGDATARSRRRR"
     gene            12675..14441
                     /locus_tag="VFR25_02535"
     CDS             12675..14441
                     /locus_tag="VFR25_02535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9939958.1"
                     /translation="MSGEATLPTPVRTEVDRLRFLVRASRELALLKRDALVRRARDLL
                     LSTGSFSELNLWVLDPRTEVLYPWALGNTPVVGRRPRLQVGQGVAGQACAEEAPAFHA
                     GVAESVFVPEEWGDRPRTLLGGVLTIPLRRGNEPFGVAVLVKADRDPLPPEEMEFFGE
                     LGIQVSIALGNARLYAEAMREKVQNQLLLELTTQISGSLEMNRLLEQILDLVFQVVRY
                     DAAGIYLVDKRTQWIHRQTLRGYDPARDEAVRLKVGKGLIGWCVKTGQGVIVSDVTTD
                     PRYMNARDETRSEMVAPIRIGSEVIGAFNLESDERDAYEAEDMELLTAFASQAAVAIE
                     RTRLHEEVLEKRRLEEEVTIGQRIQRTFLPERNPELPHFDIAGANYSSELVGGDYYDF
                     IRIADQQLGVVVGDVSGKGIAAALIMASFRASLIAEIRNNYAIRTIMTKVNRLLWESV
                     EADRFVTALYGVLDIGARHFTYVNAGHNPGFLYRAGADRFDSLDATGPLLGTLQTASY
                     KERSVDIPAGDVLVLYTDGVTEAMNGSQEFFGEERLQEVVRRKKAEPAALIVRGIWDA
                     VREFREGDQDDDLTIVVVRGTG"
     gene            14748..14834
                     /locus_tag="VFR25_02540"
     tRNA            14748..14834
                     /locus_tag="VFR25_02540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14782..14784,aa:Leu,seq:caa)
BASE COUNT         2663 a         5505 c         5518 g         2596 t
ORIGIN      
        1 cggaaacccg atacacagcc gacatcgaag ggctcccagt gggggacgcc gcgacgcgac
       61 tcacgaaggc cggcatgccc gctgaggatg ccgcacagtg gaaggagtcg ctcgagcggc
      121 tcgaacagct cgcctacgcg ccgcccgatg ctcgaggcgc gggagggacc gcgctccaga
      181 gcgccgcgga gctcgtgcgc cgcttccggg aggggctcgc gtgaggctgg ctctcttcat
      241 cgtcacggcg acgctggtcg tcgcggcgga ggggcacgcg gccaccgcac cgcctccacg
      301 cgcggatgtc ctctacaacg cggggacggc ggcgctggac aagggcgacg tcggtccggc
      361 ggtggcgttc ttgaccgcgg ccgcgaggct cgagccgcgc gcccgcgaca tccagatcaa
      421 cctcaagcag gccacggtcg cggcagcgcg cgcgcgcggc gtggagtcgc aggacgagat
      481 cgaggtgcct cgcgaggtcc cgctctcgac cgcggagatc gcttgggtcg gagccattct
      541 tctcctcgtg ggagcggcgc tcgggatcgc ctcgcgcgtc acccgtctcc ctcggaccct
      601 ggggatcgcc gcggtcctcg tcgtcgcggt gggcgccgcg atccacgtgt ggctcgtcgc
      661 gagggcgcac gccgaagccg tgcatcccga ggccgtcgtc gtcgtgccgc agctcgcggt
      721 cgagcgcggc ccggacgagc cgtcgcgcgc cccggtcgtg ctcggcgcgg gcgagcgcgt
      781 gcacctcggt ccgacccgcg gcggccaggt cgagatccgc atcgcgggag ccgcgatcgg
      841 ctgggcgccg cacgaggggc tctggcgggt ccaggagacc ccacgctata ctgcgcgcta
      901 tgccactccc tgagcgggaa cgcgcgtgag ctccgcttcg aacacggcgc tcgtcacggg
      961 cgggaccgga gggctcggtc gcgcggtggt cgcgcggttc ctcgcggacg gactcaaggt
     1021 cgcggttccg taccgctccg aggccgagtg gaccgcgctt tcgagcgcgc atccggactc
     1081 ggttcgatcg ggcgagctcc atgggttcgt ggccgacgtg accgacgagt cctcgatggc
     1141 cgacgcggca tcgagagccg cgtcggcgat gggcggcctt cgcgtgctcg cgcacatcgc
     1201 ggggggatac cggggaggcg tggacgtgga gtccgtggat gcgaatgacg tccggagcat
     1261 gatcgaggtc aatctcctct ccgcgttctg ggccgcgaag cacgtgatcc cgcacgcgaa
     1321 gaggacggga tcgggacggc tcctcttcgt ttcctcgcgc ggcgcgattc agtactaccc
     1381 cggggcggcc gcctacgccg cggccaagat cggccttcac gggctcgtcg ggacgctagc
     1441 caaggagctg ctcgcggacg gcgtgacggc gaacgcggtc ctgccgagca ccatcgacac
     1501 cgcggccaac cggtccgcga tgccgaaagg gaaattcgat gactgggtgc gccccgagca
     1561 ggtcgcggcg cttctcgcgt ttctcgcgtc ggacgcggcc tcggccgtct ccggcgcgtt
     1621 gatccccatc tacgggaggg catgatggcc cccggccaga aagtatcgaa gggacccaag
     1681 atcgcgccgg accccatccg tcccggcatc acggtcgacc agctcgtcga ccagtcgttc
     1741 ctggcctaca acggcgcccg cctccgcgag ggatgccggc tcttcgtcga gaagatgctg
     1801 gagcccgacg tgaccgtggg catcacgctg agcggcgcgc tcacgccggc cggcctcggc
     1861 cgatcgtgcc tcatcccgct cctcaaggcg ggcttcgtcg actggatcat ctcgacgggg
     1921 gcgaatctct accacgacac ccacttcggc atcggcctca cgatgcacca ggggcgctcg
     1981 aacgtggacg acgtcgcgct gcgcgagcag gggatcgtgc gcatcttcga catcttcttc
     2041 gactacgacg tgctcctctc gaccgactcc ttcttccgcg aggtcatgcg gcagccggag
     2101 ttccgcgcgc ccatgggcac ggcggagttc cactaccgcg tcgggaagta catcctcgag
     2161 cgcgagaagg cgctcgggca gaaggagacc tcgctcctcg cgcaggcgca cgcgctcgag
     2221 gtgccggtct acacgtcgtc gccgggagac agctcgatcg gcatgaacgc ggcggagctc
     2281 tggctcgacg gagcggcgat gcactgggac gtgttccgcg acgtgaacga gacggccgcg
     2341 atcgtgtacg acgccaagca gaagggcgga aagagcgcga tcctcattct gggcggcggg
     2401 tcgcccaaga atttcgcgct ccagaccgag ccgcaaatcc aggaagtgct cggcatcgag
     2461 gaagagggac acgactactt cctacaagtg acggacgccc gcgtggacac gggcgggctc
     2521 tccggcgcga cgccgggcga ggccgtgagc tggggcaaga tcgatcccga tcgccttccg
     2581 gacgcggtgg tcgcgtacgt cgattcgacc gtggcgctcc cgatcctcgc ggcgtacgcg
     2641 ctctcgaagc acgaggcgcg gcccttgaag cgcctctaca aggaccggga acggctcctc
     2701 gaccagcttc gcgccgcgca caagggcgcg cgtgcggagg ccgagccgag gggcgcgcgc
     2761 tcgtgaagac ggtcccgacc ggcactgcgt ccaccgcgac acccgtccca ctcctagacc
     2821 tcggactcgc gcatcgcgcg atcgcggagg agctgaagcg cgacttcgag cgggtgctca
     2881 cctcggggca gttcatcctc ggggcggagc acgacgcctt cgagcgcgag ctggctcagg
     2941 ccgcagggct gacccacgcg atcgggctct cgtccggaac ctcggccatc acgctcgcgc
     3001 tccaggcgct gggcgtcggg cccggggacg aggtgatcct gcccgcgttc acgtacttcg
     3061 ccaccgcgag tgccgtagcg cacgcagggg cgacgccggt cttcgccgac gtggagcccc
     3121 atcgcttcgg attggactgg gcgtcggtcg agtcgcgtct cggaccgcgc acgaaggcga
     3181 tccttgccgt gcatctgtac ggcctcgcat gcgatctcgc gccgctcacg cgcctggccc
     3241 agacgcgcgc gatcccgctc ctcgaggacg cggcgcaggc gatcggcgcg accgaccagg
     3301 gcgtgccggt ggggaaggcg gacgcgggtg cgaccttgag cttcttcccg accaagaacc
     3361 tgggagccct cggcgacgcg ggggctttcg tcacggaccg ggacgacgtc gcctggcgcg
     3421 tgcggctcct ctcgcgtcag ggagatgcgg gcgattaccg gcacaccgtc gtcggcacga
     3481 acgcccggct ggacgcactc caggcggcgt tcctgcgcac gaagctgcgc catctcgagg
     3541 cgtgggtcac cgcgcgacgc gatgcggctc aggcctatcg cgaggcgctc gccggcgtgc
     3601 cggtcacgct tcccgaagag cccgcgggcg cgaagcacac ctaccatcaa ttcacgatcc
     3661 gggcaccgcg gcgcgacgcg ctccagtcgt atcttcgcga gcagggggtc gcgacccgca
     3721 tctactaccc gatcccgctc cacatccagc ccgcgttcgc gcatttgggg ctcggtccgg
     3781 ggacgttccc ggtctcggag cagctcgcgg gcgaagtgat ctcgctgccc ctcttcccgg
     3841 ggatcacgcg ggagcagatc gagcgcgtgg catcctgcgt ccgcgcgttc tacaccgaac
     3901 gcccatcgag aggctgatcc atggccgata cgccgaaacc cgccgcggcg ccccgcggga
     3961 agcgcatcct ctggatcgtc ctcggatcca tcgcggggct cgtcgtgctc gtctacctgg
     4021 ccgcgtggct cctcctgcct aaggatttcg tcgagtccca ggcgatgaag atggcctcga
     4081 agaaccaggg cgcgaccatc cgatggacgc gcatgaccac gggactccag ggcttctcgt
     4141 tcggggtgaa gatcgaggga atgaccatcc gcatgccggc cgagggcaag ggggagccgt
     4201 ccctggacgg ccgcatcggc gaagtcatcg tgcgcttccg gctcctgccg ctcctcttcc
     4261 gcaaggtcga gatcgacgcg gcgcaggtga acggcgcggg catcgcgatg tacgagcggg
     4321 ggaagccgcc gggagaagga aagaagaagg aggagaagcc gtccttcgcg ctccatctcc
     4381 ccaagctcgg gttcgaccgc gtcgatgtcc gcagccgcga ccgctacgga tccggctacg
     4441 acctgcgcgg tctctcggga tccgtcgcca tcgatggcaa tctcgacgcg tttcgaagcg
     4501 cgaagatcga ggccaaggga gacagcgtgt cctggaagcc ttccgctcag gggaaggcgg
     4561 tccctttgcc ggggccgctc cacgcgaccg cgacgctcga gtcgcgggat caggggaagc
     4621 gctacgagtt cacccaaggg cacatcgagg tgggagcgct cacgagcgag ctctcgggct
     4681 cgttccggaa gccggccgaa ggcagcgatg ccatgccgct cgcgatcgtg atcacgggga
     4741 agccgcaggc tctcaagtcg agcgacgcgg cgttccgcgc cctcatgccg gagagcccag
     4801 ggagcactcc cgtggactgg aagacgaccg cgtcctggaa gatcgacatc ggcggcacca
     4861 gcgaggcgat ggtgcagaac ggcgaggcga atctccgccc cctctcgatc gaggcgggac
     4921 ccaacaagtt cacgctcgat cgcgtgcagg tgcgatacga gacgaccacg gatcagaagt
     4981 tcacgatgga tgcgacggga gcaggctccg gcgtggcgct ggcggtggcc gccgcgggat
     5041 cgacggcgcc cggcggcggc tcgaaaggcg atctccacgt ctccgcgccg gcgactcgct
     5101 tgaacgggct catgccgaac acgccggtct ggacgatggg cacgctcgag gtgggcgcca
     5161 cgttcgcgct cgagcccccg gccgcgccgg tcgtccgctg gacggtcacc ggaaaggacc
     5221 tcagcggctc ggtgacggga ctccagcgcc cggtgaccga tctccagttc cacatcgacg
     5281 gcgatcagca gaccgcgaac gtgcgatcga tgaccgccag ggtcggatcg tccaacgtgt
     5341 cgctcaccgg agccgtgaag caagggaagc cgctcggcac cgggacgttc cgcgcgtcga
     5401 tggacaagtt cgtcgcggag gagtgggcgg atccgaagac cgcgcaagag aaagggaagg
     5461 gcaaggactc gaaggccccg gcgccgcctc ccgcgatccc cctccgctcg ttcgacgggc
     5521 tcgtgacgat cggagaggtg cattcgggga atctgacgat ccgcaatctc tcggcaccga
     5581 tcgccttcgc gtccgacaag ctcgtcgcgg ctccaatcca gggcgacatg gggaccggaa
     5641 ccctcgcggg caaggtcgag gtggcgaaca tcacgaagga tccgtcctac gccgtgcact
     5701 tcgacctgaa gcgcgtcccg gttcgggagt tcgtcggtgg catgatgccc ttgaacctcg
     5761 ggctcaccgg attcgcgacc ggccaggcgg acatctcggg cgccggcctt cccggtctcg
     5821 cggccatgaa cacgctcacg ggagcgctcc aggggaacgt cgaggaagga aagatcctcg
     5881 agacccccac catcaacgcg ctccggtcgg cgctcggcac ggtgaacgga ccgggctcgc
     5941 tcggcgacct cgtcttcaag acgctccagt actcggccaa gatcgacaag ggccggctcg
     6001 tgctcaacca ggtgaacggc gctctcggca aggacctgat gcagctcgcg ggctccgccg
     6061 gtttcgacaa gtcgctcgac ctcgatcttc tcctcaagct cgcgccggga cggatccaat
     6121 cgagcaccgc cctgggccag ttcgccaact acgcccgcga cgagcagggg cggctcccga
     6181 tcaaggtcgc gatcacaggc accgtgacga accccaaggt gtcgctcaag gcggcatcca
     6241 cggtggaggc cgcgggaaag ggtctcgcca aggagatctt cgagaacatc atgcgcggga
     6301 accgtcccga cacaacgagg gccgacacgt ccaaggctca cgcggacaca tcccgcgccg
     6361 acacgacgcg cgccgccaag aagcccgcgc gcgcggacac gactgtctcc gagcctctga
     6421 agaaggctca agaggcgctc gagaagatct tcaagaagtg acccagcgcg gcccggctcc
     6481 ggccatcgag acccaccggc tgacgcgcct cttcaaggcg cggcggcggc aggccgaggt
     6541 ccacgctctc gaaggagtgg atctccgcgt cgagcggggc gagtgcttcg ggctcctcgg
     6601 cccgaacggc gcgggcaaga ccaccctcat caagatcctc acgacgctgc tcctcccgac
     6661 gacgggaacc gcgaccgtgg cgggtttcga cgtcgtgaac gatccgctcc aggtcaggcg
     6721 ccggatcaac atggtctcgg gcggggacac gtccggctat gggatcctca cggttcgcga
     6781 gacgctccat ctcttcgcac gcctgtacgg agtgcctcac gccgaggcgc gccgccgtgc
     6841 ggacgcgctc atggacgtga ccgggcttgg ccccaaggcg gacgcgctcc tctcctcgct
     6901 ctcgaccggc atgaagcaga agctcaactt cgcgcgcgcg ttcatgagcg atccggagat
     6961 cgtcttcctg gacgagccca ctctgggact ggacgtcgag gcggctcgcg acatccgcgc
     7021 gttcgtgacg cggtggatcg aagagcgccc ggatcggacg gtcctcctca cgacccacta
     7081 catggtcgag gcggatgagc tctgcggccg ggtcgcgatc atcgaccggg ggcagatcct
     7141 tgcgctggat acgccgcgcg cgctcaagcg cgcgatctcg agcgagccgg tgttcgagct
     7201 ccagatcgga acaggcacgg cccagttcga agaccttcgg gacgtgtatg gcgtccgatc
     7261 cctgtcgcac cacgcccatc ccgcgaccgg cgccatcgag ctccgcgtgg cggtggtcga
     7321 ggaccaggtg atcggggagg tgctcggccg gctcaaggag cgcggtgcgt ccgtcctcaa
     7381 tctcaccaag tcggagccga cgctcgagac cgtgttcgtc cacctggtcg gccgagggct
     7441 cgaggacgag gacacggacg ggcagggtgc ctagcgtgct tccatcggac tggcgcgagc
     7501 gcctgcggct caactggggc gccgcccagg cgcgcgcccg cgtgaggatc gtcgcctcct
     7561 ggcgcgagaa gtcatggatc atcggcgaga cggtgttccc gttcctcgcg atgtccgcgt
     7621 tcatcctcgt ctatcgcgga ctccacgcgc ccaagatcta cgaggcgttc gtcgtcctgg
     7681 ggggcgcgat gatcgcttac tggaacaacg tgctctggag catggcgagc cagttcttct
     7741 gggaaaagga gcagggccag ctccagctct atctgatggc gcccgtctcg cgcatgtcga
     7801 tcctcttagg catggcgctc gggggcatga tcatgaccac gagccgcgcg atcgtgatcc
     7861 tgacgggggg catcctcatc taccacgtgc cgctcccgct cgatcgcgcg tttccggcgt
     7921 tcctgatgtt cctcctcacg ctgggcgcgg tctacagcct cgggatgatc ctgagcagcc
     7981 tgttcctcct ctgggggcgc gaggtctggc acacggccac gctcttccag gagccgatcc
     8041 atctcctctc ggggttctac ttcccggtgc gggcgctcgg cgcggtggcc gccgggctcg
     8101 cgtcgctcct tccgatcacg ctcggcctgg acggcatccg ccagatcctc tacgggaagg
     8161 cggcgcacgg gctcctgccg ctcgcctgga tcccgccgct ccagatcctc ctcctcgcct
     8221 tcttcctctt cctggcgcac aagtcgctcg cgctgatgga gaacctcgga aagcgggaag
     8281 ggcggctcac gctgaggggc ggatgagccc gcctgcggaa cgccccggcg cgctgaggcg
     8341 ccaggccgac gtcatcggcg cctccgcatg gctgggctgg cagatcgacg cgaactgggc
     8401 ggacccgttc acgtttctcg tgttctcgat cgcgaagccg ctcgcgacga gcctcatcct
     8461 ctacttcatg gtgaagatcg tgtcgcccgc gagcgcgacg ggggaggcct tcctctttct
     8521 cttcctcggg aacacgttct tcctctacgt gaccgaggtc ctgatcggca tctcctggac
     8581 cgtgttccgc gaccgcgagg actacgagac gctgaagtac atctacctcg cgcccatgcc
     8641 gctccgatcg tatctcttcg ggcggggcat ggcgaagatg ggcaccgcga cgctcggcgt
     8701 gatcgtcgcg ctcctgttcg gacgcttcgt gctccacctt ccgatcggca cgccccatac
     8761 gccgtggggc ctcgtgatcc tcgccgtgct cctcggcctg gtcgcggtgg catggctcgg
     8821 gatcgtgctc gccggcgtgt cgctggtggt cgcgaggcac agcatcaact tgaacgaggg
     8881 attgagcggc gtcttctacc tactgagcgg cgcggtgttt ccgatcgagg tcttgccggg
     8941 tttcgcgcag cggatctcgc tctggctccc gttcacctac tggctcgagc tcttgcgaag
     9001 gttgatcttg gggaaatcgt tctcgaaggt gctccagggc gtgagcgacg ggaggctctg
     9061 gctcgtgctc gtcctctcga ccgtggggat cgccatattc gcgtgggtgt ggttccgctt
     9121 ctgcgagcac gtggcgcgct cgcgcggact catcgactgg aagacgaatt actgacgcgc
     9181 cgctggggcg cgggaatcag gcgggaagcg ggcggcgaac tttcttgcga agggctttgc
     9241 cgaggatcac gttcactggg tggctccgcg acagcatcgg gagcaccggc gctttccgga
     9301 tgccgagctt ccggagcgcg tcctgcgcgg cctcgaagtt cgggttccac gagagcgcct
     9361 ggttgaagca cttgatggcg tccgacttcc gcccggccat gaagtgcacc tcgccgaggt
     9421 tgtagtagtg ctcggggcgg tggtactccg cttcgatggc gcgccggcaa agctgctccg
     9481 cgtcggcgta cttccgctcc gcgtgcgcca ccgcgacgcc caggtagctc aggtacccct
     9541 tggaattcgg ctcgagcatg accgcttcct ggaagtgcac cagggcgtcc ccgtaccggc
     9601 aggcgtacat catccggacc ccggtcagga agtgctcccg cccgatggcc accacgacgg
     9661 ccagctgctt ttgacgacgc tgcttcggtg agatcatgct tggctccagg ggtggtggtt
     9721 cgcgcggagt gaaacgttcg caatcctcgt gcctggaggg ctcgacccag gggccaattg
     9781 cacggctagg taaacccgta cgggacactg gagtacgagg caagcttcag agggcctaga
     9841 cccctcgccg ggcgggtcgc gaggaggcag tgcgcaataa ggagcttgtc accagcgaat
     9901 tgcgtcttgt aaggtggggt gcgagggtca ggagccagcg ccacctaaga cccggaggta
     9961 catgacgtcc cccagcttgg ccgcttcgaa gaacgcgtcg tcgctcgaat cacttctcag
    10021 acccgtccac tttgtatccg agcttcgatc caagaagaag gcacaggtcc tcgacgagct
    10081 tgcggctgcc ttggccgcaa ccggagtgac acgctcgccc gagatcgtgg ccgagctcct
    10141 gcgcgagcgg gaagccctcg gaagcacggg cgtggggaag ggaatcgccg tgccgcacgc
    10201 tcggtccacg ctgatcgccg agcgggcgtt gctggtggcc aggtcccaga aggggatcga
    10261 gttcgacgcc gtcgatggag agccggtgca cctgctcttc ctcgtcgtgg ccccgccgac
    10321 ggagcgggac cccatctatc tcaaggtgct cgcggacgtc gtgcgtgcgg tacggctgtc
    10381 ccggacgcgt cagcgcctag gcgacgcgcc cgatttcgaa accgttcggg agatcttgat
    10441 ccatgccgcc gctgaatgag cagctcgacg cactcgtcac gcttcaagac atcgacctca
    10501 tgctccggga ctcccgcgat cccgagatgg ggacccacga ggatcgcctc ggcttcgcca
    10561 ggcagaacgt gaagggcctg gagaagaccc gggagcggct ccgcagccag atcgacgagc
    10621 cgctcctccg gacgtatgag cggatgagcc ggaggattcc ccggctcgtg gtgcctgtgg
    10681 aagggcgggt ctgccaggga tgccggatga gcctgccgac gtcctcggcc ggccgaaaca
    10741 cgcccggcac aactatcgaa acctgcgaga actgcgggcg aatcctctac cgaatctaga
    10801 ggcatttcgg gtatacttac gggtgttccg gcccatcgtg gccggccccg tcgacccgat
    10861 ccgaaggcag ttcaaggcat gctgaagaag accctggctt cggccctcgc catcatggcc
    10921 ctgggcatcg tcctcacctg tctcgcccgc tccgagcccc ctccgccgac cgtcgtggga
    10981 ccgacgcaga tcctgtacgc cgatgccgac accgtgtact tcctgcggag ccgcgagtgg
    11041 tggtcgggca atgtgaggca ccaggaaaag ctcctcctcc acggcctcaa gatgaagtac
    11101 tggcgggacc gcctgcccga cgatatgcgg ctcgttttcg acgagctggg gtatcccacg
    11161 gggagggtcc tcctcacgcc gtacgggcac accgaggagc actggtatta cggtcccgcg
    11221 gggccgccgc tgcgcttccg ggacggagtc cttctcgacc aggatcgctt cgacgcgctc
    11281 cggtcggaac cctgatcttt ccggaccacc ccgcgctcca cgtcgtctaa aatccaggca
    11341 tcgcgcatgc tgcgccgagc gccatccggc cagggggggt gggaacgatg ccgcgggtcc
    11401 atgggaaggg cgagtattgg cgacggtcga acgtccaaac aactcactgc gaattctctt
    11461 cgcgcgcgcg gcggtcctcg tgatcgcggc cgcgacgctg ctcgctctga ccggcgcccg
    11521 ccccgcggat gcgccggcga cagcacccct cgatcagatc gcccaggccc ccgtcgacca
    11581 gccgttcgag ctcccggata cgaccgaaga tgaagaggac gacgccgaca cgctggacgt
    11641 cgtgcctcca tccgcccagc agcaggcgcc gcctccggcg tccccggccg tggaggacac
    11701 gacgggcgcc ttccggcccg acttgaacac ggtccctcca ggtgcgaccg ccgactcgag
    11761 cgcaacgggc gccgcgcccg atacgctctt catgccttca ggcgtgctgc cgaccaaccc
    11821 acagcccacg accacgacca caacgcagcc gcaagcgacg cctcctcttc ctgggccgaa
    11881 gcaggaggtc aagaagccaa ggaagggact cctcggcatc catcccgtcg ccatcctctt
    11941 cggactggcg gcgctccaca tcatggtcac gcacctggcg gagtgatgcc gcgacggggg
    12001 acgggtgatg ggagcggcgt ggcgctgcgc gtccggtgga atccacccga cttcgcgctg
    12061 cgggagccgg ccgagcgcgt gctcgcggac gccgtgcgcg agctggggct cgcgggggtg
    12121 atccacgacc tccacgtgtt catcgactcg gccaatcggg acgaccacgc gtacatcgag
    12181 tggaacacgc acgaccatcg cgccgcgcgg ctctggttcg ccctcggcaa tttcgtgacg
    12241 ccgaagcggc gccgcatgtg gtcccgcacc tgggcccgcc gttcggggac gccccccttg
    12301 atggcgcggc agttctcggc gcggagcttc gcggaggcct gcctgcacga actctgtcat
    12361 ctcaaggacg atcacgagtc cggtgtcgat ctctccgggc atcccgagtc cgaccgcgag
    12421 gccttgaacg agctctggaa cgtgtggatc gacgggcggc tcaatcgccg cggccttccg
    12481 gccatgtcgc gcggcgagcg ccggcgcgtc ttcgcgcgca cgctcgcctc gacgccgcgc
    12541 tactcggccg tcggcgagcg cgtgttccgc gcgctctggc gggcggatca tctcggaccg
    12601 cgcgagcttc gcgcctacct cgaggagctg aagggacccc gtggcgacgc aacggctcgg
    12661 agccgtcgga ggcgatgagc ggggaagcga cgctgcccac acccgtgcgc acggaggtcg
    12721 atcgccttcg ctttctcgtt cgcgcttctc gggagctggc cctcctcaag cgcgacgccc
    12781 tcgtgcgccg cgcgcgcgac ctgctgctga gcaccggctc cttctcggag ctgaatctgt
    12841 gggtgctcga cccgcggacc gaggtcctct atccgtgggc tctggggaac acgcccgtcg
    12901 tgggacgcag gcccaggctc caggtgggcc agggcgtcgc cgggcaagcg tgcgccgagg
    12961 aggctccggc gttccacgcc ggggtcgccg agtccgtgtt cgtgcccgag gagtggggcg
    13021 accgccctcg tacgctcctg ggcggcgtgc tcacgatccc gttgcgtcga ggtaacgagc
    13081 ccttcggcgt ggccgtgctg gtgaaggccg atcgcgaccc gctcccgccc gaggagatgg
    13141 agttcttcgg cgagctgggc atccaggtct cgatcgcgct cgggaacgcg cgcctctacg
    13201 cggaggccat gcgcgagaag gtccagaacc agctcctcct cgagctgacc acgcagatct
    13261 cgggaagcct cgagatgaac cgcctcctcg agcagatcct ggatctcgtc ttccaggtcg
    13321 tccgctacga cgccgcgggc atctatctcg tcgacaagcg cacccagtgg attcaccgcc
    13381 agacgctgcg cggctacgac cccgcgcggg acgaggccgt gcgcttgaag gtgggcaagg
    13441 gcctgatcgg ctggtgcgtg aagacgggac agggggtcat cgtctccgac gtgaccacgg
    13501 acccgcgcta catgaacgcg cgcgacgaga cgcgaagcga gatggtggcc ccgatccgga
    13561 tcgggagcga ggtgatcggc gcgttcaacc tcgagtcgga tgaacgggac gcgtacgagg
    13621 cggaggacat ggagctcctg accgcgttcg cgtcgcaggc ggcggtcgcg atcgagcgca
    13681 cgcggctcca cgaggaagtc ttggagaagc gccggctcga ggaagaggtc acgatcgggc
    13741 agcgcatcca gcgcacgttc ctgcccgagc ggaaccccga gctcccgcac ttcgacatcg
    13801 cgggggcgaa ctactcgtcg gagctcgtgg gcggggacta ctacgatttc atccggatcg
    13861 ccgaccagca gctcggcgtg gtcgtgggcg acgtctcggg gaaggggatc gcggccgcgc
    13921 tgatcatggc ctcgttccgc gcctccctga tcgcggagat ccggaacaac tacgccatcc
    13981 gcaccatcat gacgaaggtg aaccggctcc tctgggagag cgtcgaggcg gaccgcttcg
    14041 tgacggcgtt gtatggggtt ttggatatcg gcgcccggca tttcacgtac gtgaacgccg
    14101 gccacaaccc aggcttcctg taccgggccg gggcggatcg cttcgactcg ctggacgcga
    14161 cggggccgct cctgggaacg ctccagacgg cctcgtacaa ggagcggtcg gtcgacattc
    14221 ccgcggggga cgtgctggtc ctctacacgg acggggtcac ggaggccatg aacggcagcc
    14281 aggagttctt cggggaggag cggctccagg aggtggtccg ccggaagaag gcggagccgg
    14341 cggccctcat cgtccggggg atctgggacg cggtccggga gttccgggag ggggaccagg
    14401 acgacgacct gacgatcgtg gtggtgcggg ggacggggta gagggccgat ggtggcccgg
    14461 gggtaaacga gaagggcggc gccgcctttc ggctacgccg ccctctcagg agaatggaag
    14521 ttccccgtgc aaaccgtatc ctgaacgacc tgtataaggc ccaagaaacc caccggcgaa
    14581 cgccgggtca agccgcccag ggaacgtgga ttacagggaa cttcacaccc aatgcgcaag
    14641 actttagact cgcgaactgt tagaaagttc cactggatcg ggggatgctc cagaacaact
    14701 ggaagaatta tagctaagta agtgtcgaaa agtcaattag attctgtgcc ggagtggtga
    14761 aattggcaga cgcgcgggac tcaaaatccc gtgaccgcaa ggtcgtgggg gttcgattcc
    14821 cccctccggc accacattcc catccgaggt ggaggggtcg agagaggtca gtcttacggg
    14881 gcgctgtcgc tggatgaacc gaggcgagtc aggaggcgca cgggcccgtg gcgccaacct
    14941 gactccctgt cttcacgaca cttcagagct cgggtctagc agacgcaggt cgcttctgtg
    15001 caaaagcagg tttcaatgca ggaatagtac gcagtgtcct cacagttgtt gatgcagtac
    15061 gtggcctcac aggtgtcgtt accgtatgta ttgtggcagt actcaggact gttgctataa
    15121 ccggtattac agtgcccctg tgtgttcttc gtatcgaggt gcatagagcc tccccgcgcc
    15181 tgtccggcag ccgtcggcat cagagccctc actgtagtcc ggctgaggcg caacttcctg
    15241 tcgcgtggat tcatcgttgg cttcctcccc tttggaagac cggctggtgc cgctgaagag
    15301 acagcaccgc atgcatcgta gggtctgatt ttggcgaaca caagggtggt cgagccgccg
    15361 cccgatggga tgagggaact atcgaggaga aactcccacc tccggtgcca agactgccga
    15421 tgtctgtgct gaaataaacc gtgatcagga acgcttgggc aggtgcagct acgaggcaaa
    15481 gaaagagcag cggaatgaca ggtattgcgc gcgtcatggt cccccctgga tccggctcgg
    15541 tggggggcac caccttcacc tgaccaaccg tgacagggca agcgcgaccg cgaagaacgg
    15601 cggtggtatt ggcgctggcg gtggcccgca gttcccaaac tagatcggag tcgactcctg
    15661 atgcggtccg gtcgtcaggg gattggagga agagggagga acgacttcgt cccgtagctc
    15721 tgaccgtcct caccgggatg caccggtgaa gagccgcccg cgccggcggc accgagggtg
    15781 aaggcgtttc cgctcatcgt gggtggggtc aagtaggcga tcccgatcga cggtccgcca
    15841 ccgcctccgc caccagcgcc gccgtatccg cccggacctc cgtcgccgcc ctttccgcct
    15901 gcataagctc caacggcact cacacctccc ctggcacctg ccatgttgag gccatcagag
    15961 atggctgtgc ctcgaagacc cgaagtgcca ttgcccccgg ctccgccgtt ggccgtcacg
    16021 atggtgttgc tctctacaac gacgacctcg gtctgggtga tcaagatgcc gaacgagcct
    16081 ccccctccac gtccgccgtg tccaggggag ccacccaggc ctccggatcc gccgccgccg
    16141 ccgccgccac cacagacaac cgagccgccg cctccgccac caccaccgcc ccctccgcct
    16201 gcgacgccgc gctgccccgg ggtcccggac gacggttgat agccgctggc aacgagggat
    16261 cccaacgcgc ctccaccggc gc
//
LOCUS       DASTKC010000058         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1461141, whole genome shotgun sequence.
ACCESSION   DASTKC010000058 DASTKC010000000
VERSION     DASTKC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461141"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VFR25_02545"
     CDS             complement(<1..1623)
                     /locus_tag="VFR25_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939959.1"
                     /translation="MHSPSLRRPCRLGSLIVLIFGLAAPATLHAASKVVAHTETPSSI
                     SAIYGRAVSVAGAGAGPYVIPTQTGFEVRDAAAPASGPLGVWRSPGPIREVAYGNQGV
                     FGGPELVFTFAGAQGIGIVDITTPSTPTGGASRIDPTPAVLGAVLQPADGLIASDGAS
                     LQPYRITFVFGDGIHLTPLAPISYADGRRIVAIRARADSFLVVSERGAPAPRTILTLY
                     QLQASASAPVQLREVQVPLQTPSDLAWDRDIAFVAASSGGVVIANIATGAFHTTALPG
                     FRAVRALDVNDSLVVMGVAASGLAKLRRSGALGDTLVGYTVEALQQEPMHVTLLGSRV
                     VIATQDALAASEPDEVARGAIEFRDLENPIAVEPAGGTGRVRRVVAAGGYAYVADYSG
                     GLRVYHSSATDSTLVGVLPATSSGRVLDLAVDPVRKRAYLAASSDGLEIVDVSDPASP
                     ALLGTLVLAERAAAVAIVDSTTVIVGGRGTVVGGVTVVDVSTPSAPAPLGHLFLQDPR
                     AIAVKDAVAFVADATQGLVSVDVGTPSDPSMIG"
     gene            1720..2388
                     /locus_tag="VFR25_02550"
     CDS             1720..2388
                     /locus_tag="VFR25_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET9939960.1"
                     /translation="MDHLLDKIVAPLAAFILGVIAALGYGGIVLCMTIESACIPLPSE
                     IIMPFSGYLVTTGRFTLWGITLAGAVGNVFGSWIAYWLGMKGGRPLAEGLARWKIIRI
                     EEYDRADGWLKKHGLKVAFWSRLLPIVRTFISFPAGSARVDFKKFTLYTFLGSLPWCL
                     GLGYVGVYFGENWESIREYWRKFDLVVVVGILVLFGIWLRHHFRSEPKRAEERDAQED
                     RPAV"
     gene            2426..2499
                     /locus_tag="VFR25_02555"
     tRNA            2426..2499
                     /locus_tag="VFR25_02555"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2458..2460,aa:Gln,seq:ctg)
     gene            2536..2610
                     /locus_tag="VFR25_02560"
     tRNA            2536..2610
                     /locus_tag="VFR25_02560"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2569..2571,aa:Glu,seq:ctc)
     gene            2620..>3948
                     /locus_tag="VFR25_02565"
     CDS             2620..>3948
                     /locus_tag="VFR25_02565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9939961.1"
                     /translation="MAARSLAIALLSAALFASAQAQPRETSHSRSTSTKGIPSNVERV
                     DSLAGITQYRLRSNGMNIYVSPNHAAPVVTFLVVYHVGSRNEAPGNTGSAHLLEHMLF
                     NKSTKNFGKANKKPTFQEVLYGAGADYSSTNMTTWNDRMTGYSTLPADKLELAMQIES
                     DRIARALILDSERQPEMSVVRNEYEIGENNPYRALEKSVVAAAIVAHPYHWDTIGYRS
                     DIEGVSTEKLREHYRTYFWPDNAEAVLVGDFDTAEALRLFDREFGKLPRSKAPIPKVI
                     TVEPPQEGERRVVVNRPGEVGIVELGYIRPGALDHDFIPLDLIQMILSNGVNSKLYQA
                     LVETKLATDIDAGNYTLMDPFPFLATATVAPGVTHEKVEAAMKKVLADLAANGVSQTE
                     LNRAKSQLEVVVVRQRDGTYPFASQLGEAVASANWKWFATYLDSMNSFTTA"
BASE COUNT          695 a         1324 c         1294 g          635 t
ORIGIN      
        1 gccgatcatg ctcgggtcgc tcggcgtgcc gacgtccacg ctcacgagtc cctgcgtcgc
       61 atcggcgacg aatgcgacgg cgtccttcac cgcgatcgcg cgcggatcct ggaggaagag
      121 atgtcccagc ggggctggcg cgctgggcgt cgagacatcg acgacggtca ccccgcccac
      181 gaccgtgcct cgtccgccga cgatgacggt tgtcgaatcc acgatggcca ccgcggccgc
      241 acgctccgcg agaaccagcg tgccgagcag cgcgggcgag gccggatccg agacatccac
      301 gatctcgagc ccatccgagg aagccgcgag atacgcccgc ttccgcacgg gatcgaccgc
      361 gaggtcgagc acgcgccccg acgacgtcgc ggggagcact ccgaccaggg tcgagtccgt
      421 ggcgctcgag tggtacacgc gaaggccgcc gctgtagtcg gcaacgtagg cgtatccgcc
      481 cgcagccacc acgcgacgaa cgcggcccgt ccctcccgcc ggctccaccg cgatcgggtt
      541 ctcgagatcc cggaactcga tcgcgccccg cgcgacctcg tcgggctcgc tggcagcgag
      601 cgcgtcctga gtcgcgatga cgacgcgcga gcccaagagc gtaacgtgca tcggctcctg
      661 ctggagcgcc tccacggtgt acccgacgag cgtgtcgccc agcgcgccgc tgcggcggag
      721 cttcgcgagg cccgaggcag cgacccccat gacgacaagc gaatcgttca catcgagcgc
      781 acgcacggcg cgaaagccgg gcagcgccgt cgtgtggaac gcgccggtcg cgatgttcgc
      841 gatcaccacg cccccggagc tcgcggcgac gaaggcgatg tctcggtccc acgcgagatc
      901 cgacggcgtc tggagcggaa cttggacttc gcgaagctgg accggagcgg atgcggaggc
      961 ctggagctga tagagcgtga ggatggttct cggcgcgggt gcgccacgct cgctcacgac
     1021 gaggaacgaa tcggcgcgtg cgcggatcgc gacgatgcgg cgcccatctg cgtaggagat
     1081 cggcgcgagt ggcgtgaggt ggatgccatc tccaaacaca aaggtgatcc gatacggttg
     1141 gagagaggct ccatcggaag cgatgagtcc gtccgcgggc tgcaacacgg cgccgagtac
     1201 ggcaggcgtg ggatcgatcc tcgatgcacc cccggtggga gtcgatggcg ttgtgatgtc
     1261 gacgatcccg atgccctgcg caccggcgaa tgtgaagacc aactctgggc cgccgaagac
     1321 cccttggttt ccataggcca cctcgcgaat cggccccggt gagcgccaga cgcctagtgg
     1381 cccggaggca ggcgcagcgg cgtcccgaac ctcgaacccg gtttgggtcg ggatcacgta
     1441 gggacccgcg cccgcgcccg ccacggacac ggcccggccg tagatggcgg agatcgagga
     1501 cggcgtctcc gtatgggcga cgaccttgga ggcggcatgg agcgtggcgg gggcagccag
     1561 gccgaaaatc aggacgatca ggctcccgag ccggcagggc cgtcgaagcg aaggggaatg
     1621 catgtctacc ttggacgcta gcacgcggcc gcacaacccc tcaagttgta agtctttccg
     1681 cttgtcggct cgagtccgtc tcgggtacgg ttccgcgccg tggatcacct gctcgacaag
     1741 atcgtagcgc cgctcgcggc cttcattctg ggcgtcatcg cagccctcgg ctatgggggc
     1801 atcgtccttt gcatgacgat cgagagcgcc tgcatcccgc ttccgagcga gatcatcatg
     1861 cccttctcgg ggtacctcgt gaccacgggg cggttcaccc tctggggcat cacgctggcg
     1921 ggcgcggtgg gcaacgtctt cggctcctgg atcgcctact ggctcggaat gaaggggggg
     1981 cgccccctcg ccgaggggct cgcgcgctgg aagatcatcc ggatcgagga gtacgaccga
     2041 gcggacggct ggctcaagaa gcatggcctc aaggtcgcgt tctggagccg gctcctcccg
     2101 atcgtgcgaa ccttcatctc cttccccgcc ggatcggccc gggtggattt caagaagttc
     2161 acgctctaca cgttcctcgg ctcccttccc tggtgcctcg gcctcgggta cgtcggcgtc
     2221 tacttcggcg agaactggga gtcgatccgg gagtactggc ggaagttcga tctcgtggtc
     2281 gtggtcggaa tcctcgtgct ctttggaatc tggctccggc accacttccg gagcgagccg
     2341 aagcgcgcgg aagaacgcga tgcgcaggaa gaccggcccg cggtttgacg gtcccggaaa
     2401 ccgatgctag aatcggccgc tcttttgggg gatcgtccag tggtaggaca cgtggctctg
     2461 gaccacggta caggggttcg aatcctcttc ccccagccac taacgcaatt gccgctcaat
     2521 gagccggttt tccatggcgc tatcgtctag gggttaggac gggtggttct cagccatcaa
     2581 accggggttc gaatccccgt agcgctacca tttcgccgta tggcagcgcg gtcgctcgcg
     2641 atcgcgctgc tctccgcggc tctcttcgct tccgcccagg ctcagccacg cgagacgtcc
     2701 cattcccgtt ccacctcgac gaaggggatc ccctccaacg tcgagcgggt cgattcgctc
     2761 gcgggcatca cccagtaccg cctccgttcc aacggcatga acatctacgt gtcgcccaat
     2821 catgcggctc cggtcgtgac gttcctcgtc gtctaccacg tgggctccag gaacgaggcg
     2881 ccaggcaaca ccggctccgc tcacctcctc gagcacatgc tcttcaacaa gagcaccaag
     2941 aacttcggca aggcgaacaa gaagccgacc ttccaggagg tcctgtacgg cgcgggggcg
     3001 gactattcct ccaccaacat gaccacctgg aacgaccgga tgaccggcta ctcgaccctc
     3061 cccgcggaca agctcgagct ggccatgcag atcgagtccg accgcatcgc ccgcgcgctc
     3121 atcctcgatt cagagcggca gccggagatg tccgtggtgc ggaacgagta cgagatcggc
     3181 gagaacaacc cctacagggc gctcgagaag tccgtcgtgg ccgcagccat cgtggcgcac
     3241 ccgtaccact gggacacgat cggctatcgc tccgacatcg agggcgtctc caccgaaaag
     3301 ctccgcgagc actaccgaac gtacttctgg ccggacaacg ccgaggccgt gctcgtcggc
     3361 gacttcgata cggcggaggc cctccggttg ttcgatcgcg agttcggcaa gcttccacga
     3421 tccaaagcgc cgattccgaa ggtgatcacg gtggagccgc cgcaggaagg cgagcgccgc
     3481 gtggtcgtga accggccggg cgaggtcggg atcgtggagc tcggctacat ccgtcccggc
     3541 gcgctcgacc acgacttcat cccgctcgat ctgatccaga tgatcctctc gaacggggtg
     3601 aattcgaagc tctaccaggc gctcgtcgag acgaagctcg cgaccgacat cgacgcgggc
     3661 aactacaccc tcatggaccc gtttcccttc ctcgcgaccg cgacggtggc ccccggcgtc
     3721 acacacgaga aggtcgaggc ggcgatgaaa aaggtcctcg ccgatctcgc ggcgaacggg
     3781 gtgagccaga cggagctgaa ccgcgccaag agccagctcg aggtcgtggt cgtgcgtcag
     3841 cgtgacggca cgtatccatt cgcgtcgcag ctcggcgaag cggtggcgtc ggccaactgg
     3901 aagtggttcg cgacgtacct cgattcgatg aactcgttca cgacggcg
//
LOCUS       DASTKC010000059         7681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1462906, whole genome shotgun sequence.
ACCESSION   DASTKC010000059 DASTKC010000000
VERSION     DASTKC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462906"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3590
                     /locus_tag="VFR25_02570"
     CDS             <1..3590
                     /locus_tag="VFR25_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9939962.1"
                     /translation="QLRSFAAEVTRVAREVGTDGRLGGQAVVAGVGGTWKDLTDNVNL
                     LAANLTTQVRNIAEVTTAVARGDLSRKITVDVKGEILELKNTINTMVDQLNAFAAEVT
                     RVAREVGTDGKLGGQAVVPGVAGTWKDLTDTVNVMAANLTEQVRGIVKVVTAVANGDL
                     KQNLTVKAKGEVAALADTINDMTGTLATFADQVTTVAREVGVDGRLGGQANVPGAAGT
                     WKALTGNVNLLAANLTTQVRAIAEVATAVTQGDLTRSIQVDARGEVAELKDNINTMIR
                     NLKLTTDQNREQDWLKTNLARFTGMLQGQRDLATVGRMLLTELVPLVNAHQGILYQMD
                     LSGMPRLRMLASYGNAKVEGYPDELDLGVGFVGQCAVDKRRILVTSLPPDTPPIDTAM
                     FQALPRSLVVLPVTFEGETRAVLGLASLGDFAPAQLAFLDQLTASVGIVFNSIEATMQ
                     TEGLLQQSQQLASELQFRQKELQQTNEQLALKAQQLAERNVEVERKNQEIEQARRALE
                     DKASELALTSKYKSEFLANMSHELRTPLNSILILGQQLGDNADGNLSPKQVEFARSIH
                     GAGTDLLNLITDILDLSKIESGTVTVEAEEIYFTMLLDTVRRTFRHEAENRRLTFEVQ
                     VDPQLGSGMATDPKRLQQVLKNLLSNAFKFTEHGGVRLSVAVATGGWSIGNPILDDAG
                     TVVSFEVSDTGVGIPTEKQKIIFEAFQQADASTSRKYGGTGLGLAISRELAHLLGGEI
                     QLRSTPGRGSTFTLYLPRNYAGPAAATPEETDGRAVKAPARFPSVRLPDRAIEKVPDD
                     REQLVPGEATLLVVEDDPTYARVLMDLAHDEGFKVLVAHRGSDALDLARQFMPSAVSL
                     DIFLPDMLGWTVLSHLKQDPATRHIPVQIVTLDEDRQHGLSRGAFSFLTKPTTTEGLG
                     AALSRIKQYATPRKKRLLIVEDDALEQRSISELLGHDDIEIETAGTAGEALEALRTVA
                     YDCAVVDLRLPDMSGLEMLEKLREDGSLTDLPVVVFTGKELSPEEDARLHTLARSVVV
                     KGVESPERLLDETSLFLHRVISKLPSEKQRMLDRLYRSDDDLVGRAVLVVDDDVRNIF
                     ALSSALERRGMTVLTASTGREALTTLDSTPDVAIVLMDVMMPEMDGYETMREVRKNPA
                     YRRLPIVALTARAMKGDREKCLEAGASDYLAKPVNTEQLLSVLRAWLHR"
     gene            3607..5223
                     /locus_tag="VFR25_02575"
     CDS             3607..5223
                     /locus_tag="VFR25_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9939963.1"
                     /translation="MTKTPVNILMVDDQPAKLLTYEAILGPLGENLIKASSGREALET
                     LLRTDIAIVLIDVHMPDLDGFELAQMIHQHPRHQRAAIIFVSAVHMTDIDRVRGYEVG
                     GVDYVSVPIVPEILRARVSVFADLYRKTQELENLNEELERRVSERTSELESSTERLRE
                     SEDALRETDKRKDEFLAMLAHELRNPLAPIRNAVEYLRMRAEADPSVRWSHDVIDRQI
                     DHLTRLVDDLLDVSRITRGRLEIRRMRSDLGDILRGAVDTIQASIQGRGQRLHVSLPS
                     EPVWVNADVVRMTQIFLNLLDNASKFTPEGGTIWLSSERTEGNAVVRLRDTGAGITPE
                     ELPQLFQMFYQIRRSTTGAQGGLGIGLALVRRLVELHGGTVEASSEGHDRGAEFVVCL
                     PELVDVPEAGSNGAGMAVPEPPATRRVLVVDDNVDSAESLAMLLRLGGHEIETAYDGL
                     QAVEAAERLKPDLVLLDIGLPGIDGYDAAARIREGSGGREVVLVAITGWGQDEDRRRS
                     REAGFDAHLTKPVDMAALSRLLAELPKVAV"
     gene            complement(5251..>7681)
                     /locus_tag="VFR25_02580"
     CDS             complement(5251..>7681)
                     /locus_tag="VFR25_02580"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9939964.1"
                     /translation="TRVVLKAKGKSWPLKTSEGKWGFTTATAATQIPAGSYTLEIDFS
                     NDFDRRATSLYRVESGGNAYTFTQFEAIDARGAFPCWDEPAYKIPFQVTIAVPAAHEA
                     VSNTPVMSSTTTKGVKTVVFEKTEPTSSYLLCIATGPLEYVPMQGMSIPARIVVPKGS
                     TALAQTAATMTPPLLKALEEYFGRPYPYKKLDLIAVPEFSPGAMENPGAITFGDRFIL
                     FDPKTMSTARKRLYAIFAAHEMAHQWFGDLVTMKWWDDLWLNESFADWMSYRIAGKVY
                     PELGVDEYELQHLATVMAEDSRLSTHAIRQPVTNIDNLFEGIQVTYGKGNATIGMFER
                     WMGPDTFRTAIRTYLKKYEWKNAVAEDLWDELSAAAGKDVKTPMATFLDQPGIPLVRA
                     ELLDGGKVKLSQRRFLGYGVQAPTEQRWGIPMTVRYPGASGVQTYSFLFDQPTSEIQL
                     PGVTAKAAWIDVNAAGGYYRTSVEPAVLATLSSRAQEILSTPERIRFINNIGALVLSG
                     EVKGDDFLRTLQGFSTDPRPEVINAVLASLGQVKGAFVTTDLEAPFSVYVRKVLGPAA
                     QRFGWDRKNGEEEAVSILRPTLLDWMADEGRDDAALTRGEQLAQSFRADRGSIDPSLV
                     NAALRLSAIRGKADLFDEYQKRFEQATAPTDRDPLLAAMGSFRDPALRDRALAYSLGP
                     TVRPHEVFAVAEAALGRLENRDVVAAWARTNHKAILAKIPPVYAPFLVYLGIACEQPK
                     LDQTNAFYSAPENTAPGMDVELKKVMETGGDCVKLRAREGPAVARYLTQVAQGSGGTA
                     PAAASRVSQPH"
BASE COUNT         1325 a         2600 c         2561 g         1195 t
ORIGIN      
        1 agcagcttcg atcatttgct gccgaggtga ctcgcgtcgc tcgagaagtg ggaaccgacg
       61 ggcggctcgg cggccaggcg gtcgtcgccg gcgtcggcgg cacgtggaag gacctcaccg
      121 acaacgtgaa cctcctcgcc gcgaacttga cgacgcaggt ccggaacatc gccgaggtga
      181 ccaccgccgt ggcccgcggg gacctctcgc gcaagatcac ggtggacgtg aagggcgaga
      241 tcctcgagct caagaacacc atcaacacga tggtcgatca gctgaacgcg ttcgcggccg
      301 aggtgacgcg cgtcgcgcgc gaggtcggga ccgacggaaa gctgggcggc caggcagtcg
      361 tgcccggcgt cgccggcacg tggaaggacc tcaccgacac cgtcaacgtc atggcggcga
      421 acctcaccga gcaggtgcgc ggcatcgtga aggtcgtgac cgccgtcgcg aacggcgacc
      481 tgaagcagaa cctcaccgtg aaggcgaagg gcgaggtcgc ggcgctcgcg gacaccatca
      541 acgacatgac cggaacgctc gcgaccttcg ccgaccaggt caccacggtg gcgcgcgagg
      601 tgggcgtgga cgggcgcctc ggcggacagg cgaacgtccc tggcgccgcc ggcacctgga
      661 aggctctgac cgggaacgtg aacctgctcg cggcgaatct caccacgcag gtccgcgcga
      721 tcgccgaggt ggcgaccgca gtgacgcagg gcgatctcac gcggtccatc caggtcgacg
      781 cgcgcggcga ggtggcggag ctcaaggaca acatcaacac gatgatccgc aacctcaagc
      841 tcacgaccga tcagaaccgc gagcaggact ggctcaagac caacctcgcg cggttcaccg
      901 ggatgctcca ggggcagcgg gacctcgcca ccgtgggacg gatgctcctc acggagctcg
      961 tgccgctggt caacgcgcac cagggcatcc tctaccagat ggacctctcc ggcatgccgc
     1021 ggctccgcat gctggcgagc tacggcaacg cgaaggtgga gggctacccg gacgagctcg
     1081 atctgggcgt cggcttcgtg ggccagtgcg ccgtggacaa gcgccgcatc ctggtcacga
     1141 gccttccgcc cgacacgccg ccgatcgaca ccgcgatgtt ccaggcgctg ccgcggagtc
     1201 tcgtcgtcct gccggtcacc ttcgaagggg agacgcgagc cgtgctgggc ctcgcgtcgc
     1261 tcggggactt cgctcccgcc cagctcgcct tcctcgacca gctcacggcc agcgtcggca
     1321 tcgtgttcaa ctcgatcgaa gccacgatgc agaccgaggg actcctccag cagtcccagc
     1381 agctcgcgag cgagctccag ttccggcaga aggagctcca gcagaccaac gagcagctcg
     1441 ccctcaaggc gcagcagctc gccgagcgca acgtcgaagt ggagcgcaag aatcaggaga
     1501 tcgagcaggc gcggcgcgcg ctcgaggaca aggcgagcga gctcgcgctc acgtccaagt
     1561 acaagtccga gttcctcgcc aacatgtccc acgagctccg cacgccgctc aactcgatcc
     1621 tcatcctggg ccagcagctc ggcgacaacg cggatggaaa cctgtcgccg aagcaggtcg
     1681 agttcgcgcg cagcatccac ggggccggca cggacctcct caacttgatc accgacatcc
     1741 tcgacctctc gaagatcgag tcggggacgg tgacggtgga ggccgaggag atctacttca
     1801 cgatgctcct cgacacggtc cgccgcacgt tccggcacga ggccgagaac cggaggctca
     1861 cgttcgaggt ccaggtggat ccgcagctcg gaagcggtat ggcgaccgat ccgaagcggc
     1921 tccagcaggt gctcaagaac ctgctctcga acgcgttcaa gttcaccgag cacggcgggg
     1981 tgcggttgag tgtcgcggtg gcgacgggcg gctggagcat cggcaatccg atcctcgacg
     2041 acgcgggaac ggtggtgtcg ttcgaggtgt cggacacggg cgtcggcatc ccgaccgaga
     2101 agcagaagat catcttcgag gcattccaac aggcggacgc gagcacgagc cgcaagtacg
     2161 gcggcaccgg gctcggactc gccatcagcc gcgagctcgc gcacctcctt ggcggcgaga
     2221 tccagctccg ctccacgccg ggccgcggct cgacgttcac gctctacctg ccgcgcaact
     2281 atgccggacc ggcggccgcg acgccggaag agaccgacgg ccgtgctgtg aaggcgccgg
     2341 cgcgattccc ctcggtgcgg cttccggatc gcgcgatcga gaaggtgccg gacgaccgcg
     2401 agcagctcgt tccgggagaa gccacactcc tcgtggtcga ggacgacccg acctacgccc
     2461 gcgtgctcat ggatctggcg cacgacgaag ggttcaaggt cctggtcgcg caccgcggct
     2521 cggacgcgct ggatctggcg cgccagttca tgccgtccgc ggtgtccctc gacatcttcc
     2581 tgccggacat gctcggctgg acggtgctga gccatctgaa gcaggatccg gcgacgcggc
     2641 acatccccgt ccagatcgtc acgctggatg aagaccggca gcacggcctc tcgcgtggcg
     2701 ccttctcgtt cctcaccaag ccgacgacga cggaggggct cggagccgca ctctcgcgca
     2761 tcaagcagta cgcgacgccc cggaagaaga ggctcctcat cgtggaagac gacgcgctcg
     2821 agcagcggag catctcggag ctcctggggc acgacgacat cgagatcgag accgccggca
     2881 ccgccggcga ggcgctcgaa gcgcttcgga ccgtcgcgta cgactgcgcg gtggtcgacc
     2941 tcaggcttcc ggacatgtcg gggctcgaga tgctggagaa gctccgcgag gacggctccc
     3001 tgaccgacct tccggtcgtg gtcttcaccg gcaaggagct gtcgcccgag gaggacgccc
     3061 ggctccacac cctcgcgcgg agcgtcgtcg tcaagggcgt cgagtctccg gagcggctcc
     3121 tggacgagac atcgctcttc ctgcaccgcg tgatctcgaa gcttccgtcc gagaagcagc
     3181 gcatgctgga ccggctctac cgctccgacg acgacctggt cggccgggcg gtgctggtcg
     3241 tggacgacga cgtccggaac atcttcgccc tgtcgagcgc gctcgaaagg cgcggcatga
     3301 cggtgctcac ggcgagcacg gggcgcgaag cgctcaccac gctcgactcc acgccggatg
     3361 tcgcgatcgt gctgatggac gtcatgatgc ccgaaatgga cggctacgag accatgcggg
     3421 aagtgcggaa gaacccggcc taccgccgcc tccccatcgt cgcgctcacg gcgcgcgcga
     3481 tgaaggggga ccgcgagaag tgcctcgaag ccggcgcctc ggactacctg gccaagccgg
     3541 tcaacacgga gcagctcctc tcggtgctcc gcgcgtggct gcaccgttga tccaggggac
     3601 gatcccgtga ccaagacgcc cgtcaacatc ctgatggtgg acgatcagcc cgcgaagctc
     3661 ctcacctatg aggcgatcct cggaccgctg ggtgagaacc tcatcaaggc gagctccggg
     3721 agggaggcgc tcgagaccct gctccgcacc gacatcgcaa tcgtcctgat cgatgtccac
     3781 atgcccgatc tggacggctt cgagctggca cagatgatcc atcagcatcc gcgccaccag
     3841 cgcgccgcga tcatcttcgt ctcggccgtt cacatgaccg acatcgaccg cgtgcgcggc
     3901 tacgaggtcg gcggcgtgga ctacgtctcg gtgcccatcg tgcccgagat cctgcgcgcc
     3961 cgggtcagcg tgttcgcgga cctttaccgc aaaacccagg agctcgagaa cctcaatgag
     4021 gagctggagc ggcgcgtcag cgagcgcacc tcggagctcg agtcatcgac cgagcggctc
     4081 cgggagtcgg aggacgctct gcgcgaaacc gacaagcgga aggacgaatt cctggcgatg
     4141 ctcgcgcacg agcttcgaaa tccgctcgct ccgatccgga acgcggtcga gtacctgcgc
     4201 atgcgcgccg aggcggaccc gtccgtccgg tggagccacg acgtcatcga ccggcagatc
     4261 gaccacctca cccggctcgt ggacgatctc ctcgacgtga gtcgcatcac gagggggcgc
     4321 ctcgagattc gccgcatgcg atcggacctg ggagacatcc ttcgcggcgc cgtggacacg
     4381 atccaggctt caatccaggg acgaggccag aggctccacg tgtcgcttcc gtcggagccg
     4441 gtctgggtga acgcggacgt ggtgcggatg acgcagatct tcctcaacct cctcgacaat
     4501 gcctccaagt tcacgcccga gggaggcacg atttggctct catcggagcg gaccgagggg
     4561 aacgcggtgg tccgcttgcg ggacacggga gcggggatca cgcccgagga gctgcctcag
     4621 ctcttccaga tgttctacca gatacgacgc tcgacgacgg gcgcgcaggg aggactcggg
     4681 atcgggctcg cgctcgtccg gcggctcgtg gagctccacg gtggaacggt cgaggcgtcg
     4741 agcgaagggc acgaccgggg ggccgaattc gtcgtctgtc ttccggagct tgtggacgtg
     4801 ccggaggcag gatcgaacgg cgccgggatg gctgtgccag aaccgcccgc cacgcggcgg
     4861 gtcctcgtcg tcgacgacaa cgtcgactcc gcggagagcc tcgccatgct tctccgcctc
     4921 ggtgggcacg agatcgagac cgcctatgac ggcctgcaag ccgtggaagc cgcggagcga
     4981 ctaaagcccg atctcgtcct gctcgatatc ggacttcccg gaatcgacgg ttatgacgcc
     5041 gcggcgcgca tccgggaggg ctccggcggc cgcgaggtgg ttctggtcgc aatcacggga
     5101 tggggtcagg atgaggatcg tcgccggtct cgtgaagcag gcttcgacgc ccacctcacg
     5161 aaaccggtcg acatggcggc cctgagtcgc ctcctcgcgg agctcccgaa ggtcgccgtc
     5221 tagcgcgcgt cttgcgcgcc gccgcgctcg tcagtgcggc tgcgacaccc ggctggcggc
     5281 cgccggcgcg gttccgccgg atccctgcgc cacctgcgtc agatagcgcg ccaccgcggg
     5341 gccctcgcgc gccctcagct tcacgcagtc accgcctgtc tccatcacct tcttcagctc
     5401 gacgtccatc ccaggagccg tgttctcggg cgccgagtag aaggcgttcg tctggtcgag
     5461 cttgggctgc tcgcaagcga tccccaggta cacgaggaac ggcgcgtaca ccggcgggat
     5521 cttggccagg atggccttgt ggttcgtccg cgcccacgcg gccaccacgt cgcgattctc
     5581 gaggcggccc agcgcggctt ccgcgaccgc gaatacctca tgggggcgga ccgtcggtcc
     5641 gagcgagtag gcgagagcgc gatctcggag cgccggatcg cggaagctcc ccatcgccgc
     5701 gagcaaggga tcgcgatccg tgggcgccgt cgcctgctcg aagcgcttct ggtattcgtc
     5761 gaagagatcc gccttcccgc gtatcgccga gagtcggagc gccgcgttca cgagggacgg
     5821 atcgatggag ccgcggtcgg cccggaagga ctgcgcgagc tgctcgcccc gcgtgagcgc
     5881 cgcgtcatcg cgtccctcgt ccgccatcca gtcgaggagc gtgggacgca ggatggacac
     5941 ggcttcttct tcaccgttct tccggtccca tccgaatcgc tgcgcggccg gcccgagcac
     6001 cttccgtacg tagaccgaga acggagcctc cagatcggtg gtgacgaagg cccccttcac
     6061 ctgcccgagc gatgccagca ctgcgttgat cacctcgggg cgcgggtccg tggagaagcc
     6121 ctgaagcgtg cgcaggaagt cgtccccctt cacctctccg gacaggacga gcgcgccgat
     6181 gttgttgatg aagcggatcc gctccggcgt gctcaagatc tcctgcgcgc gcgaggagag
     6241 cgtggcgagg accgccggct cgacgctcgt gcggtaataa ccgccggcgg cgttgacatc
     6301 gatccaggcc gccttcgcgg tgactcccgg gagctggatc tccgaggtcg gctggtcgaa
     6361 gaggaacgag taggtctgga ctccggatgc gcccggatag cgcacggtca tcgggatgcc
     6421 ccaccgctgc tccgtcgggg cctggacgcc gtaaccgagg aaacggcgct gcgagagctt
     6481 caccttcccg ccatccagga gctccgcgcg cacgaggggg atgccgggct gatcgaggaa
     6541 ggtggccatc ggcgtcttga cgtccttgcc cgcggccgcg gaaagctcgt cccagagatc
     6601 ttcggccacc gcgttcttcc attcgtactt cttgagatac gtccggatcg ccgtgcgaaa
     6661 cgtgtcgggg cccatccacc gctcgaacat gccgatcgtg gcgttcccct tcccgtaggt
     6721 gacctggatc ccctcgaaga ggttgtcgat gttcgtcaca ggctggcgga tcgcgtgcgt
     6781 gctcaaacgc gaatcctcgg ccatcacggt cgccaggtgc tggagctcgt attcatcgac
     6841 tcccagctcg ggatagacct tccccgcgat ccgatagctc atccaatccg caaacgactc
     6901 gttgagccac aggtcgtccc accacttcat cgtgaccaga tcgccgaacc actggtgcgc
     6961 catctcgtgc gcggcgaaga tcgcgtagag gcgcttccgc gccgtgctca tggtcttcgg
     7021 atcgaacagg atgaaccggt cgccgaacgt gatcgcaccc ggattctcca tcgcgccggg
     7081 cgagaattcc ggcaccgcga tcaggtcgag cttcttgtag gggtacgggc gtccgaagta
     7141 ctcctcgagc gccttgagga gcggcggcgt catggtcgcg gccgtctgtg cgagcgccgt
     7201 gctccccttc ggcacgacga tacgggccgg aatcgacata ccctgcatgg gcacgtactc
     7261 gagcggcccg gtggcgatgc acaggaggta cgacgaggtc ggctcggtct tctcgaacac
     7321 gactgtcttc acgcccttgg tggtcgtcga gctcatgacc ggtgtgttcg agacggcctc
     7381 gtgcgccgcg ggcactgcga tcgtcacctg gaagggaatc ttgtacgccg gctcgtccca
     7441 gcaggggaac gctccgcgcg cgtcgatcgc ctcgaactgg gtgaacgtgt acgcgttccc
     7501 ccccgattcc acgcgataga gcgaggtggc gcgccggtcg aagtcgttcg agaaatcgat
     7561 ctcgagcgtg tagctcccag ccgggatctg ggtcgccgcg gtcgcggtcg tgaagcccca
     7621 cttgccttca gatgtcttga gcggccagga cttccctttc gctttcaaca cgacgcgcgt
     7681 g
//
LOCUS       DASTKC010000060        15629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_517680, whole genome shotgun sequence.
ACCESSION   DASTKC010000060 DASTKC010000000
VERSION     DASTKC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15629
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517680"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..813)
                     /gene="bla"
                     /locus_tag="VFR25_02585"
     CDS             complement(58..813)
                     /gene="bla"
                     /locus_tag="VFR25_02585"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HET9939965.1"
                     /translation="MVRPLAWAAFAASLLVAAAAPAVMKSAAPLKSTVKLRLLRPGVW
                     IHTSRYVFPDGSVIPANGLVVKEGGGLVLIDTAWGEMLTADLLDEIKREIKLPVRRAI
                     VTHSHADRIAGADLLRQRGIPVFAHPLTVRKAASVAIAMPTDSLAGLRKPGDAVFVGS
                     VEVFYPGPAHSPDNIVVWVPSARVLFGGCAVKALDSNLGNLADADTRAWPDAMHRVME
                     RYRQAEVVVPGHGAEGGQQLLTHTLELLSESRS"
     gene            complement(819..1220)
                     /locus_tag="VFR25_02590"
     CDS             complement(819..1220)
                     /locus_tag="VFR25_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939966.1"
                     /translation="MPRRVRLALIAMSLSVLIVTALPIVRELTRRTDIWWTPRTMLVP
                     LADSADRVEIYAHGQPLAKLIQAGHVQVLDEGRPVSLGAEDVGLRFNNSERVRAERLP
                     RLLVSSALFGAAVLYLLFLVTGRLVYREERA"
     gene            complement(1244..1639)
                     /locus_tag="VFR25_02595"
     CDS             complement(1244..1639)
                     /locus_tag="VFR25_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939967.1"
                     /translation="MKRLRILAGIIAALLTIYAVVGITGLMKGFDLIGAMLVALAATL
                     AWLGWWFVIRGWRVVQARLKWALIGGIVVGGVGFIIGFLGPLLWAPDANQGPLLGIFI
                     TGPIGFILGAVAGAILGKPRERDATTSTK"
     gene            complement(1636..2295)
                     /locus_tag="VFR25_02600"
     CDS             complement(1636..2295)
                     /locus_tag="VFR25_02600"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET9939968.1"
                     /translation="MSGAGPDESLPKLRPIDPAGIPSADVYALMTGCVVPRPIAFVSS
                     LSVSGVPNLAPFSFFNAGGANPPSLVFAPITSGAGRDKDTLHNIAATREYVVHIVPWS
                     LREHMNQTSADYPPETDEFEMAGFTKAPSVKVKPWRAAECPIAMECRLFKIVEHGEGP
                     FRANYVIGEVVLFHVAEALLSGLRVDAASIDAIARLGGPNYTRVTKESVFAMPRPVLP
                     K"
     gene            complement(2292..3482)
                     /locus_tag="VFR25_02605"
     CDS             complement(2292..3482)
                     /locus_tag="VFR25_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET9939969.1"
                     /translation="MPIYHTLGNVPHKRHTQFRRPDGKLYTEQLMGSRGFSGRSSLIY
                     HHNMPTQAREIRKIQDCRVTYADEQTLRHHHFKTKGLQKSGDQVSGRVTLLHNNDVSM
                     AIAVPDQTMAYFYRNGQGDDMYFVHEGTGRIQTIFGELHYRPGDYVVIPRGTTYRFHP
                     DEGTQRYLVIESASSIETPKRYRNEYGQLLEHSPFTERDIRKPERLETFTDKGTFEVR
                     IKARDQITAYEFDFHPLDVVGWDGYLYPYIFNIEDFEPITGRIHMPPPTHQCFEGRNF
                     VVCSFVPRLYDYHPDALPVPYNHSNVDSDEVLYYVNGNFMSRRGIEVGSFTLHPSGIP
                     HGPHPGAAEASIGAKETKELAVMLDTFYPLRVTTDAMPYDDKSYPYSWLEPPKSFTGE
                     GATG"
     gene            complement(3492..4460)
                     /locus_tag="VFR25_02610"
     CDS             complement(3492..4460)
                     /locus_tag="VFR25_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9939970.1"
                     /translation="MRLVSFSPEGTAARALGALVDDSVLELARAAAALDVPESDPALL
                     STMETLLADWDHGLLVTRETVARATRLSGPLRERLAQSIHPRASVTLHPPVAHPPSLR
                     DFYAFETHVKNARARRGLTVPEEWYQFPAFYFSNPGALVADGEPVTKPAWTEAMDYEL
                     EIACVISHEARDVRAENWRSVVAGFTILNDWSARDVQRKEMAVGLGPAKGKDFATSLG
                     PALVTLDELEPSREGDRHNLAMEARVNGRTLSRGNARDLHFTFGQMIARASQDVYLFP
                     GDVIGSGTVGGGCILELGTETHRWLAPGDEVTLEIERLGALTNRIV"
     gene            complement(4468..5562)
                     /gene="hppD"
                     /locus_tag="VFR25_02615"
     CDS             complement(4468..5562)
                     /gene="hppD"
                     /locus_tag="VFR25_02615"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET9939971.1"
                     /translation="MSANDDFLKIRSVDHVEFWVGNARQASFYYVNAFGFTVTGYAGL
                     ETKVRDRASYLLQQGKIRFVMTAPLSPEGPIAEHVHRHGDGVKDIALEVEDVDRAYQE
                     TTKRGARGVMEPTTISDDHGSIRRAAIATYGDTIHSFIDRSKYKGPVLPGYKPTGTRP
                     ADGPLLYVDHMVGNVALGDMNKYVAYYRDVMGFSQFQHFDDKDISTEYSALMSKVMAD
                     GSGKVKFPINEPAEGKRKSQIEEYLDYYKGAGVQHVALATSDIISTVARLRESGVPFI
                     SVPYSYYEVLEDRVGKIDEDKAALAKLGILVDRDEEGYLLQLFTKPVQDRPTVFYEVI
                     ERKGSRGFGKGNFKALFEAIEREQALRGNL"
     gene            5691..7562
                     /locus_tag="VFR25_02620"
     CDS             5691..7562
                     /locus_tag="VFR25_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9939972.1"
                     /translation="MGAWTLTALAGLYVALVWMATNAAGPRNWGIHSAAFLPPTLRVA
                     LLAAMAAAVAALALAARESRAPHRPTEPRREPRSKRKTQAPQSTQRTSRRKAPLWLLA
                     LPLYGVLLYVLRTRTHFLGDGGVWLNILLGGNAETFSEPLSSVAWHGYRTVARALGFP
                     MTDATFALFPILCGVAFAGLGWLLLGEALPRGSRGTRALAWGLLLTIGTTQLYYGYIE
                     TYPIASVVVLAYLWLALRHVRGADPPWLLPLVYTITIGFHLMAVVLFPSYAMAVFKRP
                     GTSAVKAALLPAPLILAPLLLTATGVEPSQWMRPFELAARSAAGGGAALTRPYPLLST
                     AHAVDVANALLLAAPIPFLMGLSWLVGRRGRIRPARPASQVLAVAGLAAVAALLVLVL
                     PVAPAQDWDLTAFLLLPATLAALTAGMTMAPRPLAAGALLTLSAASLLAFVLVNATEA
                     ASIARYKTLLAPEAPLSPYGRGYGHSMLSEFYEDRGVMDSALVYAELAIRSEPTNPRY
                     WLREGTILYEQKRYDEAIPKLEEAIQRGTTRVAAHFNLGLAYARTQRFGEAADQFRTA
                     MAMDSTNPEYPHHLALMLYDEGFPDSARGMWEGVLERWPGYALTARALARRFPAQTP"
     gene            complement(7516..8721)
                     /locus_tag="VFR25_02625"
     CDS             complement(7516..8721)
                     /locus_tag="VFR25_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939973.1"
                     /translation="MPARRVAIIGAGPIGLEAALEAARRGFEVRVYEAGQAGEHLRRF
                     AAIPLFTPFGMNSSLEGRERLRSEGAALPSDSTILTAGDLVERYLVPLTRLPELAASI
                     REQERVTHVGREGLGKAAGDGRGGRGFLLRVEADGATRFDRTDFVVDATGVYGNPNAT
                     GPGGLPALGEDRLGDAIARHWDVRIHSANGPYARGTTLLIGDGHSAATALLAFDQVAR
                     ADASFRVHWISRMRRVAFPEIDGDSLPARLDLARRANEIASSAPWLTRDPGVHVVAYE
                     RRGDRVRVALTDGSSLDVDRVLALVGYRPDVAMTRELQVHYCYASEGTMKLAAAILGA
                     GAGGDCTAQVAHGPESLQNPEPDFFVLGAKSYGRNPQFLLSIGHQQVRDAFGLMGSGP
                     GIAAPALSP"
     gene            complement(8721..9431)
                     /locus_tag="VFR25_02630"
     CDS             complement(8721..9431)
                     /locus_tag="VFR25_02630"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET9939974.1"
                     /translation="MNAEARAAALVEEANDALDEGDPTTALDRFEAALRDDPEHWGAA
                     LGRVEALHHLWRPDEALREVRALAPPRGEEDDPDRAELEGRILEVLGDLEGADRLFAE
                     AHRLEPDDFSLPVRMNEADFKTILDRVLESMPQVIRETIVQVPVLVETKPSAEVADRA
                     PQITPDIVGLFVGTTVGEKITAPSGYPDVVLLFQRNLERAGRSRTEVAREIKITLLHE
                     YGHYLGFDEEELEHLGLG"
     gene            complement(9439..9885)
                     /locus_tag="VFR25_02635"
     CDS             complement(9439..9885)
                     /locus_tag="VFR25_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939975.1"
                     /translation="MRLHARLPALVAASVLVLGLASCQQLAGTGKGSAQFVELRTQLA
                     GTREALRKAVGESDVTRVGTLLPALSSRLDEIEKRSSAMNMLDREHVALEIATARRTI
                     TETDRWVAAGDLDSVRLKISQIDASLGEIDQILDRTIRGATDQSTS"
     gene            complement(9855..10631)
                     /locus_tag="VFR25_02640"
     CDS             complement(9855..10631)
                     /locus_tag="VFR25_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9939976.1"
                     /translation="MSFAAEHHSHDPVPSPWPIVAAFGLGFVPIGLILWSHGHPQGLA
                     VLAVGLAITLFSASKWWATLLLDRFVGRDLVDADSRQKRAFLMFILSEAAIFSAFFAA
                     IIYSRVHTKIWPPEGMPHFEVLLPAVNTFLLVTSSVTLHLGHTALERGRKDGVVASLL
                     MTIFLGALFLCGQAYEYGFLNGSAFSIKSGIFGATFFMLTGFHGLHVLVGIIFLNVVY
                     GAAVKGRLTPEQNFPFQAASWYWHFVDVVWLCVFTLVYLL"
     gene            complement(10741..11226)
                     /locus_tag="VFR25_02645"
     CDS             complement(10741..11226)
                     /locus_tag="VFR25_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939977.1"
                     /translation="MSPKGRGRRLLAALTFIAVASSVATTHRSSRAAELQILDETQAT
                     EAQILDSVILRWTAPGDDGLTGRAAHYDLRYSLSSITGTDTLGWWNSANRISIWNKVP
                     ALPSKPDSVRALGFTPGYRYYAMIRTCDDASNWSPFSNIAVIQASDLTPPKAVTDLRV
                     R"
     gene            complement(11448..14669)
                     /locus_tag="VFR25_02650"
     CDS             complement(11448..14669)
                     /locus_tag="VFR25_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9939978.1"
                     /translation="MKLSDLSIQRPVFATVMSLAIILFGVIAFTRLPVREYPDIDPPI
                     VSVTTFYRGASPSVVETEITDILEEQFATIEGIKTIDSSSREQGSVITIEFELSRNVE
                     EAANDVRDRVSRVRGTLPTEADDPIVAKVDVNAQPIFWLALSSDRHNGLELTEVGDLT
                     LKEQIQRFPGVASVFIGGERRYAMRVWLDAQRMANRGVTTQDVERAVKAENAEIPGGR
                     VEGIGREFAVRTRGELTTADEFGAIIVAQRGSDIVRLSDIAEVQVGPEDERSLARWNG
                     QQAVGLGIVKQKTASTLQVAKEVREAIPELSKLLPQGMKLDVAYDSSTFIQDSIDEVS
                     HTILIAMCLVILVILLFLKSFRATLIPSVAIPVSIIGALAVAYFLGFTINILTLLAMV
                     LAIGLVVDDAIVVLENVYRHMEMGKSRMKAAFDGADEIGFAILATTISLVAVFIPVAF
                     LQGSVGRLFNEFGLTVAVAVAISGFVALSLTPMLTSRMLRPLHGGGKGWASRTFDNFF
                     HWLDRVYDRLLRGAIHRPLLVGGIALLFVGVSVVCFMFMRKELAPTEDRGVAFGIVIA
                     PEGSTLAYTDKYMRQVEAILLPMPERAGLFTATGLGFGGPGRVTNGFLFFRLKPLGER
                     PKAWIPTPLPMLPMVQVPIYRAKSAQEVVQQLFPQLISIPGVLAFVINPPSLGGQFSS
                     TPIEYVLEADDYGTLGQAVGAMMAEGQKLGYLVNMDTDLRLNTPQLDIGIDRDRAAQL
                     GVSVTDIGSTLETLLGGKAISEFKRGTKQYDVIAQLKPTARATPEAIEGIYLRGANGL
                     VQLASVINIKETVAPKELNHYNRQRSATITANLAPGVSLGQALDDLDRISATALPSGV
                     KHELAGQSKEFRESSNSLYLLFGLAVVFIYLVLAAQFESFIHPLTILFSVPLAVVGAL
                     ISLFIFGQSMNIFSQIGLIMLIGLVTKNAILIVEYANQLRGRGQELTEAVIHAAEIRL
                     RPILMTSLATIFGVLPLAIGIGAGAESRRPLGIAVVGGMLFSTFLTLLIVPLVYRLLG
                     RYTHLNTAEGGAGSAETSVSHEAPAPAHAPAAASVLEPTRTAH"
     gene            complement(14674..>15629)
                     /locus_tag="VFR25_02655"
     CDS             complement(14674..>15629)
                     /locus_tag="VFR25_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9939979.1"
                     /translation="LPFHEGQPVAKGTVLARLDDSQLKAEVDRAAATRDQAKSSYDRV
                     KEVVEQKAGSAQDMDDAAAALKVADANLALARTRLSKTRIVAPFSGVVGSKKVSPGAY
                     LRVGTEITDLAQLAELKVIFSAPERYLATLKRGSEVTVSTPAFPNTVLSGRIDVVNPV
                     LDPGTRSAQVIARVRNPRGEFRPGMSANVSAVLSERMNALTIPNEAVFAEGNQTYVYV
                     VGPDSTVARTAVQLGTRLSDVVEVVSGLETGARIVVAGHQKLFDKARVIPIDSHADQS
                     AAGGSTAPGGAAGAAAAPAKPESGPKGDASSKPAASGTTRK"
BASE COUNT         2528 a         5194 c         5362 g         2545 t
ORIGIN      
        1 gtcaccttag cccctgagca tggtagaggt gcgattcgtt cgagatggag ctaagcatca
       61 cgagcgagat tccgagagga gctcgagcgt gtgtgtgagg agctgctggc caccctccgc
      121 gccgtgtccc ggcacgacta cctcggcttg gcgatagcgc tccatgacgc gatgcatcgc
      181 gtccggccat gcccgcgtgt ccgcgtcggc gagattgccg aggttcgagt ccagcgcctt
      241 cacggcgcag ccgccgaaga gcacgcgggc cgacggcacc cacacgacga tgttgtccgg
      301 cgaatgcgcc ggccccgggt agaagacctc caccgatccc acgaaaactg cgtcccctgg
      361 cttgcggagc cccgcgaggc tgtccgtcgg catggcgatc gccacggaag ccgccttccg
      421 gaccgtgagc gggtgcgcga acaccgggat cccgcgctgg cgcaggagat cggcgcccgc
      481 gatgcggtcg gcgtgggagt gcgtcacgat ggcgcggcgc accggcagct tgatctcccg
      541 cttgatctca tcgaggagat cggccgtgag catctcgccc caggccgtgt cgatgagaac
      601 gagtccaccc ccctccttca ccaccagccc gttcgcgggg atgaccgatc cgtccggaaa
      661 gacgtaccgc gacgtatgaa tccagactcc ggggcggaga aggcgaagct tgacggtgga
      721 cttgagaggg gccgcggact tcatgaccgc cggggccgcc gcggccacga ggagcgaggc
      781 cgcgaacgct gcccacgcga gtggccgcac catggacgtc acgcccgctc ctcccggtag
      841 acgagacgtc cggtgacgag gaagagaaga tagagcaccg ccgcgccgaa gagcgccgag
      901 gagacgagca gcctcggcag gcgctccgcg cggacgcgct ccgagttgtt gaatcggaga
      961 ccgacgtctt cagcacccag actcaccggc cggccctcat cgaggacctg cacgtggccc
     1021 gcctggatga gcttcgcaag cggttgtccg tgtgcgtaga tctcgacccg gtccgcgcta
     1081 tcggcgagcg ggacgagcat cgtgcgcggg gtccaccaga tgtccgtccg ccgggtgagc
     1141 tcgcggacga tgggaagcgc ggtcacgatc aggactgaga gactcatggc gatcagggcg
     1201 agccgtacgc gacgaggcat cgttcctccc tccgtgggcg ggcctatttc gtcgaggtcg
     1261 tggcgtcgcg ctcgcggggc tttccgagaa tggcgcccgc caccgcgccg aggatgaatc
     1321 cgatcgggcc ggtgatgaag atcccgagga gtggaccttg attggcgtcg ggagcccaaa
     1381 ggagcggacc gaggaacccg atgatgaatc ccactccccc cacgacgatc ccaccgataa
     1441 gcgcccactt gagccgggcc tgcaccactc tccatccacg aatgacaaac caccacccga
     1501 gccaggccaa ggtcgccgcg agagcgacga gcatcgcgcc gatgagatcg aatcccttca
     1561 tcaagcccgt gatgcccacg acggcgtaga tggtcagcaa ggccgcgatg atcccggcca
     1621 ggattcgcag gcgtttcact tcggcagcac cggccgtggc atcgcgaaca cgctctcctt
     1681 cgtgacgcgc gtgtagttcg gcccgccgag acgcgcgatc gcgtcgatcg acgccgcgtc
     1741 cacccggagc ccgctcaaga gcgcctccgc gacgtggaac aggacgactt caccgatgac
     1801 gtagttggcc cggaagggac cctccccgtg ctccacgatc ttgaagagcc ggcactccat
     1861 cgcgatcggg cattccgcgg cgcgccaggg cttcaccttg acgctcggcg ccttggtgaa
     1921 gccggccatc tcgaactcat ccgtctcggg cgggtagtcc gccgaggtct gattcatgtg
     1981 ctcccgcagg gaccacggca cgatgtggac gacgtattcc ctcgttgcgg cgatgttgtg
     2041 gagggtgtcc ttgtcgcgcc ccgcgccgct cgtgatcggg gcgaagacga gcgacggcgg
     2101 attcgcgccg cccgcgttga agaaggagaa cggcgccaga ttcggcacgc cggaaaccga
     2161 gagtgaggaa acgaacgcga tcggccgcgg aaccacgcag ccggtcatga gcgcgtagac
     2221 gtcggcggac gggatgccgg cgggatcgat ggggcgaagc ttgggaagcg actcgtccgg
     2281 gcccgccccg ctcacccggt ggcgccctct cccgtgaagc tcttcggcgg ctcgagccag
     2341 gagtacgggt agctcttgtc gtcgtacggc atggcatcgg tcgtgacgcg gagcggatag
     2401 aacgtgtcga gcatcacggc gagctccttc gtctccttcg ccccgatcga tgcttcggcc
     2461 gcgccgggat gcggtccgtg cgggatcccc gacggatgga gcgtgaacga gccgacctcg
     2521 atgccgcgcc ggctcatgaa gttcccgttc acgtagtaga gcacctcgtc cgagtccacg
     2581 ttggagtggt tgtacgggac cggcagtgcg tcgggatggt agtcgtagag gcgcggcacg
     2641 aacgagcaga ccacgaagtt ccgcccttcg aagcactggt gcgtcggcgg cggcatgtgg
     2701 atgcgccccg tgatcggctc gaaatcctcg atgttgaaga tgtagggata gagatagccg
     2761 tcccagccga ccacgtcgag cggatggaag tcgaattcgt aggccgtgat ctggtcgcgc
     2821 gccttgatcc ggacctcgaa cgtgcccttg tcggtgaagg tctcgaggcg ctcgggcttc
     2881 cggatgtcgc gctccgtgaa cggggagtgc tcgaggagct ggccgtactc gttccggtac
     2941 cgcttcgggg tttcgatgga cgacgcgctc tcgatgacga ggtagcgctg cgtcccctcg
     3001 tccggatgga acctgtacgt cgtgccgcgc gggatcacga cgtagtcgcc ggggcggtaa
     3061 tggagctcac cgaagatggt ctggatgcgc ccggtgccct cgtggacgaa gtacatgtcg
     3121 tccccctggc cgttccggta gaagtacgcc atcgtctggt ccggaaccgc gatcgccatg
     3181 gagacgtcgt tgttgtggag gagcgtgacg cggccgctca cctggtcgcc gctcttctgg
     3241 agacccttcg tcttgaagtg atggtggcgg agcgtctgct cgtcggcgta ggtcacgcga
     3301 cagtcctgga tcttccggat ctcgcgcgcc tgcgtcggca tgttgtggtg atagatcaag
     3361 gaggagcggc ccgagaagcc tcgggagccc atcagctgct ccgtgtagag cttcccgtcg
     3421 ggcctgcgaa actgggtgtg ccgcttgtgc gggacgttcc ccagggtgtg atagatcggc
     3481 atgcgtgtgc ctcagacgat tcgattggtg agagcgccga ggcgctcgat ctcgagcgtc
     3541 acctcgtcgc cgggggcgag ccagcgatgc gtctccgtgc cgagctcgag gatgcagccg
     3601 ccccccacgg tcccggaccc gatgacgtcg cctgggaaaa gatacacgtc ctgcgacgcc
     3661 ctcgcaatca tctgcccgaa cgtgaaatgg aggtcgcgag cgttgccgcg cgagagcgtg
     3721 cggccgttca cgcgcgcctc catggcgagg ttgtgccggt ccccttcgcg gctcggctcg
     3781 agctcgtcca gcgtgacgag cgcggggccg agcgacgtcg cgaagtcctt ccccttcgcg
     3841 gggccgagcc cgaccgccat ctccttccgc tgcacgtcgc gcgcgctcca gtcgttcagg
     3901 atcgtgaagc cggccacgac ggaccgccag ttctccgcgc gcacgtcccg cgcttcgtgc
     3961 gagatcacgc acgcgatctc gagctcgtag tccatggcct cggtccaggc cggcttcgtc
     4021 accggctcgc catcggccac gagcgctccc ggattgctga agtagaaggc cgggaactga
     4081 taccactctt ccggcaccgt gaggccgcgc cgcgcgcgcg cgttcttcac gtgggtctcg
     4141 aaggcgtaga agtcgcgaag gctgggcgga tgtgcgacgg gcgggtggag cgtcaccgac
     4201 gcgcgcgggt ggatggactg cgcgaggcgc tccctcaagg ggccgctgag cctcgtagcg
     4261 cgcgccacgg tctcgcgggt cacgagaagg ccgtgatccc agtccgcgag cagcgtctcc
     4321 atcgtggaga ggagcgcggg atcggattcg ggcacgtcga gcgccgccgc ggcgcgcgcc
     4381 agctcaagca cggagtcgtc gacgagcgcc ccgagggcgc gcgcggccgt cccctccggg
     4441 gagaaggaga cgaggcgcat ggaggggcta gaggttgccc cggagcgcct gttcccgctc
     4501 gatggcttcg aacagggcct tgaagttgcc cttaccgaag ccgcggctcc ccttccgctc
     4561 gatcacctcg tagaacacgg tgggccggtc ctggaccggc ttcgtgaaga gctgaagaag
     4621 gtacccctct tcgtcgcggt cgacgaggat cccgagcttc gcgagagccg ccttgtcctc
     4681 gtcgatcttc cccacccggt cctcgagcac ctcgtagtac gagtagggga cgctgatgaa
     4741 ggggacaccc gactcgcgga gccgcgcgac cgtcgagatg atgtcgctcg tggcgagcgc
     4801 cacgtgctgc acgccggcgc ctttgtagta gtcgaggtat tcctcgatct gcgacttccg
     4861 cttcccctcg gccggctcgt tgatcgggaa cttcaccttg cccgacccgt ccgccatcac
     4921 cttcgacatg agcgcggagt actcggtcga gatgtccttg tcgtcgaagt gctggaactg
     4981 gctgaagccc atcacgtcgc ggtagtaggc aacatacttg ttcatgtcgc cgagcgcgac
     5041 gttcccgacc atgtgatcca cgtaaagaag cggaccgtcc gcggggcgcg ttccggtcgg
     5101 cttgtagccg ggcaggaccg ggcccttgta cttcgagcga tcgatgaagg aatggatcgt
     5161 gtcgccgtag gtggcgatcg ccgcgcgccg gatcgagccg tgatcgtccg agatcgtcgt
     5221 cggttccatg acgccgcgcg cgccgcgctt cgtggtctcc tggtacgcgc ggtcgacgtc
     5281 ctcgacctcg agcgcgatgt ccttcacgcc gtcgccgtgg cgatggacgt gctccgcgat
     5341 gggcccttcg gggctgagcg gggccgtcat gacgaatcgg atctttccct gctggagcag
     5401 gtaggacgcg cggtcgcgga ccttggtctc gagcccggcg tagccggtga cggtgaaccc
     5461 gaacgcgttc acgtaataga acgaggcctg gcgggcgttc cccacccaga actcgacgtg
     5521 gtcgacggag cggatcttca ggaaatcgtc gttcgcggac atcccgcggc ctcctcgaag
     5581 cagggtccgg cccccggtcg ggggcggaca aggttggtgt gggcttgatc ttccgccatt
     5641 gtacgccccc cggggggccg cgcaacgcgg aaagcgtata ctgccgccgc atgggagcgt
     5701 ggacgctcac ggcgctggcc ggcctgtacg tcgccttggt ctggatggcc acgaatgcgg
     5761 cgggaccccg gaactgggga atccactccg cggccttcct cccgccgacc ttgagagtcg
     5821 cgctcctcgc ggccatggcg gccgcggtgg cggcgttggc gctcgcggcg cgcgaatccc
     5881 gcgcgcccca ccgaccgacg gagccccggc gagagccgcg atcgaaacga aagacgcagg
     5941 cgccgcaatc cacgcagcgc acctcgcgcc gcaaagcacc gctctggctg ctcgcccttc
     6001 ccctgtacgg ggtcctgctc tacgtccttc gaacgcgcac tcactttctc ggcgacggcg
     6061 gcgtgtggct caacatcctc ctcggaggga acgccgagac cttcagcgag ccgctgagct
     6121 ccgtcgcatg gcatggctat cgcaccgtcg cccgcgcgct gggatttccg atgacggacg
     6181 cgaccttcgc tcttttcccg atcctctgcg gcgtcgcttt tgcgggcctg ggatggctcc
     6241 ttctcggcga ggcgcttcca cgcggctctc gaggaacgcg ggccctcgcg tggggactcc
     6301 tgctcacgat cgggaccacg cagctctact acggctacat cgagacctat ccgatcgcga
     6361 gcgtcgtcgt gctcgcctat ctgtggctcg cgctgcgcca cgtgcgcgga gccgacccac
     6421 cgtggctcct ccccctggtc tacaccatca ccatcggatt ccatctcatg gcggtcgtcc
     6481 tctttccctc gtatgcgatg gccgtcttca agcgcccggg cacgagcgcc gtgaaggcgg
     6541 cgctcctccc cgctccgctc atcctcgctc cgctcctctt gaccgctacg ggagtggaac
     6601 cctcccagtg gatgcgtcca ttcgagctcg cggcgcgctc ggcggcaggt ggaggagccg
     6661 cgttgacccg gccctacccg ctcctgtcga cggcgcacgc ggtcgacgtc gcgaacgccc
     6721 tcttgctcgc cgcaccgatt ccattcttaa tggggctgtc gtggctcgtg ggccgacgcg
     6781 ggcgaatccg tccggccagg cccgcatcgc aggtgctcgc cgtcgcaggg ctcgcggccg
     6841 tggcggcgct cctcgtcctg gtgctcccgg tcgcgccggc gcaggattgg gacctgacgg
     6901 cgttccttct cctgccggcc acgctcgcgg ctctcacggc cggcatgacg atggcacctc
     6961 ggccgctcgc ggccggcgcg ctcctcaccc tctccgccgc gtccctcctc gcgttcgttc
     7021 tcgtaaacgc gacggaggcg gcgtcgatcg cgcgctacaa gacgctcctc gctcccgagg
     7081 cgcctctctc cccgtacggc cgcggctacg gccacagcat gctgtcggag ttctacgaag
     7141 atcgcggggt gatggactcg gctctcgtct acgcggagct cgcgatccgg tcggagccca
     7201 cgaacccgcg ctactggctg cgcgagggga cgatcctcta tgagcagaag cggtacgacg
     7261 aagcgatccc gaagctcgag gaggcgatcc agcgaggaac cacgcgggtg gccgcgcact
     7321 tcaatctggg cctggcctac gcgcgaacgc agcgattcgg tgaggcggcg gaccagttcc
     7381 ggaccgcgat ggcgatggac agcacgaacc ccgaataccc gcatcacctc gcgctgatgc
     7441 tctacgacga gggcttcccc gattcggctc gtggaatgtg ggagggcgtg ctcgagcgct
     7501 ggcccggcta cgccctcacg gcgagagcgc tggcgcggcg attcccggcc cagaccccat
     7561 gagcccgaac gcatcgcgca cctgctggtg tccgatcgag aggaggaact gggggttccg
     7621 cccgtagctc ttcgctccga gcacgaagaa gtcgggttcg ggattctgga gcgattcggg
     7681 gccgtgggcg acctgcgctg tgcaatcgcc accggcgccg gctcccagga tcgcggccgc
     7741 gagcttcatc gtgccttccg acgcatagca gtagtgcacc tggagctcgc gcgtcatggc
     7801 gacgtcggga cgatagccga cgagcgccag cacgcgatcg acgtcgaggc tcgagccgtc
     7861 cgtgagggcc acgcggacgc gatctccgcg ccgttcgtac gcgaccacgt ggacgccggg
     7921 atcacgcgtg agccacggcg cgctcgaggc gatctcgttg gctcggcggg ccaggtcgag
     7981 acgggccggg agcgaatctc catcgatctc agggaacgcg acgcgacgca tccgcgagat
     8041 ccagtgaacg cggaacgacg cgtccgcgcg cgcaacctga tcgaaggcga ggagcgcggt
     8101 cgcggcagag tgtccatccc cgatcaggag cgtcgtgccg cgagcgtacg gcccgttcgc
     8161 ggagtggatc cggacgtccc agtgccgcgc gatggcatcg ccgagacggt cctcgccgag
     8221 ggcgggcaac cctccaggcc cggtcgcgtt cgggtttcca tagacgccgg tcgcgtccac
     8281 gacgaagtcg gttcgatcga agcgcgtcgc tccgtcagcc tccacgcgaa gcaggaaccc
     8341 acgccctccg cggccgtcac cagcagcttt gccaagaccc tcgcgtccga cgtgggtgac
     8401 gcgctcctgc tcgcggatcg aggccgcgag ctcgggcagc cgggtcagcg gcacgaggta
     8461 gcgctcgacc aggtctcccg cggtcaggat ggtggaatcg gaagggagcg ctgcgccctc
     8521 ggagcggagc cgttcgcgcc cttccagcga ggagttcatg ccgaacggag tgaagagagg
     8581 gatcgctgcg aaccggcgga ggtgctcgcc ggcctggccc gcctcgtaga cccgaacctc
     8641 gaagccgcgc cgcgcggcct cgagcgccgc ctcgagaccg atggggccgg ccccgatgat
     8701 ggcgacccgc cgggccggca tcagccgagt ccgaggtgct cgagctcctc ctcgtcgaac
     8761 ccgaggtagt ggccgtattc gtggagaagg gtgatcttga tctcgcgcgc cacttcggtg
     8821 cgggagcgcc cggcgcgctc gaggttcctc tggaagagga gcacgacgtc gggatagccg
     8881 gagggcgcgg tgatcttctc ccccaccgtc gtgcccacga agagccccac gatgtcgggc
     8941 gtgatctgcg gcgcgcgatc ggcgacttcg gcgctgggct tggtctcgac cagcaccggc
     9001 acctggacga tcgtttcgcg gatcacctgg ggcatcgact cgagcacgcg atcgaggatg
     9061 gtcttgaagt cggcctcgtt catccggacc ggaagcgaga agtcgtcggg ctcgagccgg
     9121 tgggcttccg cgaagaggcg gtcggcccct tcgagatcgc ccaggacttc gaggatgcgt
     9181 ccctcgagct ccgcgcggtc gggatcgtcc tcttccccgc gaggcggtgc gagcgcgcgc
     9241 acttcgcgaa gcgcttcgtc cgggcgccag aggtgatgga gggcttcgac gcggccgagc
     9301 gccgcgcccc agtgctcggg atcgtcgcgg agcgcggcct cgaagcgatc gagcgcggtc
     9361 gtcggatcgc cctcgtcgag ggcatcgttc gcctcttcga cgagtgctgc ggcgcgggct
     9421 tcggcgttca tgggagcctt aggaggtgga ctggtcggtc gcgccgcgaa tcgtgcgatc
     9481 gaggatctgg tcgatctcgc ccaggctggc gtcgatctgg ctgatcttga gccgcacgga
     9541 gtcgaggtcc cccgcggcga cccaacggtc ggtctcggtg atggtgcgcc gcgccgtcgc
     9601 gatctcgagg gcgacgtgct cgcggtcgag catgttcatg gccgagcttc gcttctcgat
     9661 ctcgtcgaga cggctcgaaa gggcgggaag gagggtgccg acccgcgtga cgtccgactc
     9721 tccgaccgcc ttccggagag cctcgcgcgt ccccgcgagc tgggtgcgaa gctccacgaa
     9781 ctgggccgac cccttccccg tgccggcgag ctgctggcac gaggcgagcc ccaggacgag
     9841 gaccgaggcc gcgactagag caggtagacg agcgtgaaga cgcatagcca caccacgtcg
     9901 acgaagtgcc agtaccagga agccgcctga aacgggaaat tctgctcggg cgtgaggcgg
     9961 cccttcacgg cggcgccgta gaccacgttc aaaaagatga ttccgacgag cacgtgcagc
    10021 ccgtggaagc cggtgagcat gaagaaggtc gcgccgaaaa tcccgctctt gatgctgaac
    10081 gcggagccgt tcaggaatcc atactcgtac gcctgcccgc agaggaaaag cgcccccagg
    10141 aagatggtca tgaggagcga agcgacgacc ccatctttcc gaccccgctc gagcgccgta
    10201 tggcccaggt gaagcgtgac gctgctcgtg acgaggagga acgtgttgac ggccgggagc
    10261 aggacctcga agtgcggcat cccctccggc ggccagatct tggtgtgcac ccgcgagtag
    10321 atgatcgccg cgaagaaggc gctgaagatc gcggcctcgg agaggatgaa catgaggaac
    10381 gcgcgcttct gtcgcgagtc ggcgtccacg aggtcgcgcc ccacgaagcg gtccaggagg
    10441 agcgtcgccc accacttgct ggcgctgaag agcgtgatgg cgaggcccac cgcgaggacg
    10501 gccaggccct gcggatgccc atgagaccac aggatgagac cgatcggcac gaatccaagt
    10561 ccgaacgcgg ccacgatggg ccacgggctt ggaacgggat cgtgggaatg atgctcggca
    10621 gcgaacgaca tgcgcgtcct ccgaatcagg tcgggccgga attcggcggc catgatgccc
    10681 gggaacggag tcgcccgcaa gcgttttccc gatccacgac cgaggccggt tgcccctggc
    10741 tcaacgcacc cggaggtcgg tgaccgcctt cggcggcgtg agatcggacg cctggatgac
    10801 cgcgatgttg gagaacgggc tccaattcga cgcatcgtcg cacgtccgga tcatcgcgta
    10861 gtagcggtag ccgggcgtga agccgagcgc ccgcaccgaa tcgggcttgc tgggcagggc
    10921 cgggaccttg ttccagatgg agatcctgtt cgcgctgttc caccatccga gggtgtctgt
    10981 gcccgtgatc gaactcaggc tgtagcggag atcgtagtga gctgcgcgac cggtcagacc
    11041 atcgtcgccg ggagcggtcc aacgaaggat cacggagtcg aggatctggg cttcggtggc
    11101 ctgcgtttcg tcgagaatct ggagctccgc cgcccgactc gagcggtggg ttgtggcgac
    11161 actcgatgcg acagcaatga aggtgagagc ggcgagcaaa cgacggcccc gtcccttggg
    11221 cgacatgaat cctccgaagt tgtctggcaa tggatgctga ggatggccgc cgggggaggc
    11281 gactgcgggc gcctaacgca gttttcgtgc cgaacccttg ggtctgcgtc gcatgcggcg
    11341 caaatcacgc atcgtcggcg cgggcgcacg cgaggaacgc acgcgtaagc gcaacgtgtg
    11401 taccggaatg ctcggcggcc gcaccgaagt ggcgcagccg tgcgcgatca gtgcgccgtg
    11461 cgcgtgggct cgagcacgct cgcggcggcg ggagcgtgcg ccggagcggg tgcctcgtgt
    11521 gacacggatg tctcggcaga gcccgcaccg ccttctgccg tattcaggtg cgtgtagcgc
    11581 ccgaggaggc ggtagacgag cggaacgatg aggagggtga ggaaggtgga gaagagcatt
    11641 ccgccgacga ccgcgatgcc gagcggccgg cgcgactcgg cgcccgcgcc gatgccgatc
    11701 gcaagcggaa gcacgccgaa gatcgtggcg agcgaggtca tgagaatcgg ccggagacgg
    11761 atctcggccg cgtggatcac cgcctccgtg agctcctggc cgcggcctcg gagctggttc
    11821 gcgtactcca cgatcagaat cgcgttcttg gtcacgagcc cgatcagcat gatgagcccg
    11881 atctgcgaga agatgttcat gctctgcccg aagatgaaga gcgagatcag ggccccgacg
    11941 actgcgagcg gcaccgagaa gaggatcgtg agcgggtgga tgaagctctc gaactgcgcc
    12001 gcgagcacga gatagatgaa gacgacggcc aagccgaaca ggagatagag gctgttgctc
    12061 gactcgcgga actccttcga ctgccccgcc agctcgtgct tgacccctga tgggagcgcc
    12121 gtggcagaga tccggtcgag atcgtccagc gcctgcccga ggctcacccc gggcgcgagg
    12181 ttcgctgtga tggtcgcgga gcgctgccgg ttgtagtggt tgagctcctt gggcgccacc
    12241 gtttccttga tgttgatgac gctcgcgagc tgcacgagcc cgttcgcgcc ccgcaggtag
    12301 atcccctcga tcgcctcggg cgtggcgcgc gcggtcggct tgagctgcgc gatcacgtcg
    12361 tactgcttcg tgccccgctt gaattcggag atcgccttgc caccgaggag ggtctcgagc
    12421 gtgctcccga tgtcggtcac cgacacgccg agctgcgccg cgcgatcgcg gtcgatcccg
    12481 atgtcgagct gcggcgtgtt gagacggaga tcggtgtcca tgttgaccag atagccgagc
    12541 ttctgcccct ccgccatcat ggcccccacc gcctgcccca gcgtcccgta atcgtcggcc
    12601 tcgagcacgt actcgatcgg ggtcgacgag aactgcccgc ccagggacgg cggattgatg
    12661 acgaacgcga gcacgccggg aatcgagatg agctgcggga agagctgctg cacgacctcc
    12721 tgagccgact tggcgcggta gatggggacc tgcaccatgg ggagcatcgg gagcggcgtc
    12781 gggatccatg ccttcggtcg ctcgccgagc ggcttcaacc ggaagaagag gaacccgttc
    12841 gtcacgcgtc cgggcccacc gaaacccagg ccggtcgcgg tgaacagccc cgcgcgctcc
    12901 ggcatcggca ggaggatggc ttccacctgg cgcatgtact tgtcggtgta ggcgagcgtc
    12961 gacccttccg gcgcgatgac gatgccgaac gcgacgccgc ggtcttctgt gggcgccagc
    13021 tccttccgca tgaacatgaa gcacacgacg ctcaccccga cgaacaggag cgcgatcccc
    13081 ccgacgagca acggccggtg gatggccccg cggaggagcc ggtcgtagac gcggtccagc
    13141 cagtggaaga agttgtcgaa cgtccgcgac gcccagccct tgccgccgcc atggagcggc
    13201 cggagcatgc gcgatgtgag catcggggtg agcgagagcg cgacgaagcc cgagatcgcg
    13261 accgccaccg cgaccgtgag tccgaactcg ttgaagagcc gtcccaccga tccctgcagg
    13321 aacgccacgg ggatgaacac ggcgacgagc gagatcgtcg tggcaaggat cgcgaatccg
    13381 atttcgtccg ccccgtcgaa cgccgccttc atgcgcgact tccccatctc catgtgccgg
    13441 tacacgtttt ccagcacgac gatggcgtcg tccaccacga gcccgatggc taggaccatg
    13501 gccaggagcg tgaggatgtt gatcgtgaaa ccgaggaagt aggccaccgc gagcgcgccg
    13561 atgatcgaga ccgggatcgc gaccgaggga atgagcgtgg cccggaagct cttgaggaag
    13621 aggaggatca cgaggatcac gaggcacatg gcgatcagga tggtgtgcga gacctcgtcg
    13681 atcgagtcct ggatgaaggt cgatgaatcg taggcgacgt cgagcttcat tccctggggg
    13741 aggagcttcg agagctcggg gatcgcttct cgaacctctt tggcgacctg gagggtgctc
    13801 gccgtcttct gcttcacgat gccgagtccc accgcctgct gtccgttcca gcgggccagg
    13861 ctccgctcgt cctcggggcc gacctgcacc tcggcgatgt cggagagccg gacgatgtcg
    13921 gatccccgct gcgcgacgat gatcgcgccg aactcatcgg ccgtggtcag ctcgccccgc
    13981 gtccgcaccg cgaactcgcg cccgatccct tcgacccgtc cgcccgggat ctccgcgttc
    14041 tcagccttga ccgcgcgttc cacgtcctgc gtcgtgacgc cccggttggc catgcgctgc
    14101 gcgtcgagcc agacgcgcat ggcgtaccgg cgctcgccgc cgatgaagac cgacgccacg
    14161 cccgggaagc gctggatctg ctccttgagc gtgaggtctc cgacctcggt cagctcgagt
    14221 ccgttgtgcc ggtcgctcga gagggcgagc cagaagatcg gctgggcgtt gacgtccacc
    14281 ttggcgacga tgggatcgtc cgcctcggtc ggcagcgtgc cccgcacgcg cgacacgcgg
    14341 tcgcggacgt cgttcgccgc ttcctccacg ttgcgggaga gctcgaactc gatcgtgatg
    14401 accgacccct gctcgcggct cgacgagtcg atcgtcttga tcccctcgat ggtcgcgaac
    14461 tgctcctcca ggatgtccgt gatctcggtc tcgaccacgc tcgggctcgc gccgcgatag
    14521 aaggtcgtca ccgacacgat cggcggatcg atgtccgggt actcacggac gggaagccgc
    14581 gtgaacgcga tcaccccgaa gaggatgatc gcgaggctca tgaccgtcgc gaagacggga
    14641 cgctggatgg agagatcgct cagtttcatg ggtctacttc ctcgtcgttc cggacgccgc
    14701 cggcttgctc gaggcgtccc ccttgggtcc tgattcgggc ttggccggag ccgccgcggc
    14761 gccggccgct ccgccgggag ccgtgctgcc tccggccgcc gactgatccg cgtgcgaatc
    14821 gatcgggatc acgcgcgcct tgtcgaagag cttctgatgt ccggccacca cgatgcgcgc
    14881 acctgtctcg agcccgctca ccacttcgac cacgtccgag agccgggtcc cgagctggac
    14941 cgccgtgcgc gccacggtcg agtccggtcc gaccacgtac acgtaggtct gattcccctc
    15001 cgcgaacacg gcctcgttcg ggatcgtgag ggcgttcatg cgctcgctca ggacggccga
    15061 gacgttggcc gacatgcccg gacggaactc gccgcgaggg ttccgcacgc gcgcgatcac
    15121 ctgagcgctt cgcgtccccg gatcgagcac cgggttgacc acgtcgatcc gaccgctcag
    15181 gaccgtgttc gggaacgccg gcgtcgacac cgtgacctcc gatccgcgct tcagtgtcgc
    15241 caggtagcgc tcgggagcgg agaaaatgac cttgagctcg gcgagctgcg cgaggtcggt
    15301 gatctcggtg cccacccgca gataggcgcc ggggctcacc ttcttcgagc cgaccacacc
    15361 cgagaacggg gcgacgatcc gcgtcttcga gagccgcgtg cgcgcgagcg ccaggttcgc
    15421 atccgcgacc ttgagcgcgg ccgccgcgtc gtccatgtcc tgcgcggagc ccgccttctg
    15481 ctccacgacc tccttcaccc ggtcatacga ggacttcgcc tgatcgcgcg tcgccgccgc
    15541 gcggtcgacc tcggccttga gctgggagtc gtcgaggcgc gcgaggaccg ttcccttggc
    15601 gaccggctga ccctcgtgga acggaaggc
//
LOCUS       DASTKC010000061         6538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1228621, whole genome shotgun sequence.
ACCESSION   DASTKC010000061 DASTKC010000000
VERSION     DASTKC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228621"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFR25_02660"
     CDS             complement(<1..594)
                     /locus_tag="VFR25_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9939980.1"
                     /translation="MIARRDVSARACYAPRMPTPAAIPITVVPAVPLLALDTVWFQVA
                     GTLCNLTCSHCFITCGPTNRRHAMMSRAETRPYLDEALALGVRDFYFTGGEPFLNREL
                     LSILEDALAMAPSTVLTNATLIDAREASRLRALERESRYSLEMRVSLDGLDPASNDAL
                     RGAGSFAEALAGLRELSQAGFDPIVTVAQTWSDADDDV"
     gene            635..1642
                     /locus_tag="VFR25_02665"
     CDS             635..1642
                     /locus_tag="VFR25_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9939981.1"
                     /translation="MIWDVVIIGAGPAGLSAALFTRMRRMSTLMIETAVAGGQLASLY
                     PKKPVHDMPSYTYVMGEDLGRNFVEQAAHMGVEIHEGEHVTSIARDDDAAVVRVVSTK
                     GSYEARSVIVATGGGAFEPRKINKPGEAELKGRGVTYGMPDIEESRGKRVLVIGGGDS
                     ALESALSLKDVAQVTLIHMLDKWQGMDGYVEDVRESSIRALLETETVEIKGNGRVETV
                     VVRNKKTGDTEELPIDLVSINIGYLMDTKIVRQWGLELEGSQIKVDPIMKTNITGVFC
                     CGDIATYPGKYKLLITACSEGAAAANTAYIHVKQPKKFTVGELYAAPKEEGDTQPKAG
                     E"
     gene            complement(1682..3025)
                     /locus_tag="VFR25_02670"
     CDS             complement(1682..3025)
                     /locus_tag="VFR25_02670"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9939982.1"
                     /translation="MPISRPEEVLNNVRSMAERSGAAEAETYFEFVRFVEVRYREGEV
                     ELLQQSAITGLGLRVLRDRKMGFLYTTNLNRTVLDELVVRTIALASQATPRDENKLPD
                     QTFPPQTNLEIEDPYADRITPQDLIVLAKALEDNALLVNKVKTTQTARAGYVVGETHF
                     SNTFIPYQTFRNTQFWLYCSAVATDGSQKRVGSFTDRKRNFQDLTTPDRVGRKAGERA
                     VARLGAKPVPSARVPVIFEAEAAGGFLSGLFGAFVGLNVLEQRSFLAGKKGQTIGSPL
                     VTLVDDAILRRGLGTRPFDGEGTQTSRRIVADRGVLTRFLHTAATARREGVTPSGSVW
                     RGYDSLPAVGPSNFYLENGSTKLSTMIEQVPNGLFVTGTAGFGFDLAAGDYSQQVEGN
                     WIEKGKLGRPVDGITVAGKISDMLMGVDAVGRDLDFRAQVCSPSIRFKELTVGGG"
     gene            complement(3027..4574)
                     /locus_tag="VFR25_02675"
     CDS             complement(3027..4574)
                     /locus_tag="VFR25_02675"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9939983.1"
                     /translation="MPIDEPISRRDFLRHGAVLSGAAMAAGASGLSWLKPPDADAAIM
                     GIGKELRDVLNLETVNQILIEALKRGGDFADLFAEQRFRTNIVLDDQKIDSVTYGYPR
                     GAGVRVFFRRQTGYAFSDEIAYNDLLDAAHVASAVVLNQGTVAPVDVRPRVLTPPFSV
                     MNPVALMNENTKFDILRRMDAAARSVSPKVVSVRIEYQDEARDTLIGTSDGGYAIDRQ
                     YLVSISCSATAVNGPDRRTGRSFLGGRVGEDYFLRRDPEAVAKDAALQAVTLLGADPA
                     PAGAMPVVIAPGWGGVLIHESFGHALEGDSIRRGTSLLSGLMGQKIGSPHLRVIDDAL
                     WPNGRGSFTVDDEGTPGQRTVLVDGGYLQSYILDRQSASLLNLKSTGNGRRMSYRQWP
                     LPRMTNTYIDRGTDDPASLLSGITKGFYAADLGGGSVDTTSGNFNFAVQLGYMIENGK
                     RTKPVRGAVLIGNSLETMQRIEGIGSDLQVDQTRGLCGKDGQMVPVGVGQPTVRFASI
                     TVGGTAI"
     gene            4670..5065
                     /locus_tag="VFR25_02680"
     CDS             4670..5065
                     /locus_tag="VFR25_02680"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET9939984.1"
                     /translation="MDEANLLLPELERALDGLAALGKEITALRREIEVLAAIESSGAD
                     AANPDVRELRDREKACAARMEIFRRSLEEISRHGCIIRDLDMGLVDFYTVSKGQVVCL
                     CWRRGEPQIEHWHPVNEGFSGRRPLDDIA"
     gene            5106..>6538
                     /gene="polX"
                     /locus_tag="VFR25_02685"
     CDS             5106..>6538
                     /gene="polX"
                     /locus_tag="VFR25_02685"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET9939985.1"
                     /translation="MDKHQVARTLEEIAILLELKGENSFKIRAHENAARAVDGLAEDL
                     AVLVQEGRLTSVPGIGDSIAKKIAELWTTGRMKHYDELAAEVPRGYLDMIRVPGLGAK
                     KIRALGEALQITSLERLKEACEAGEVRVLKGFGEQSERKILQGIALVLQGAGRYLAAE
                     VRPMAEELLSYLRGHASVKAAEVGGSLRRWMETVKDVDLVVATTKPAAVAKAFLEILP
                     MHTITGAGDTKTSVRLPNGLAIDLRLVSPKEFPFALHYFTGSAAHNIRVRGRALDRGW
                     SLNEYTLLAEAAKVKKAPSVADEADLFRALGLAYIEPELREDRGEIEAAEAGTLPELI
                     TLADMKGILHCHTVASDGKSTLEEMAEAAIAHGASYLGISDHSASARYANGLSEEALA
                     EQGAAIDRWNKKSKKLRILKGTETDILPDGTLDYPDHVLAKLDFVIASVHTHFTMDEA
                     AMTARVVRALRNPFVSVLAHPTGRLLL"
BASE COUNT         1104 a         2167 c         2246 g         1021 t
ORIGIN      
        1 gacgtcgtcg tccgcgtcgc tccacgtctg tgccacggtg acgatgggat cgaagcctgc
       61 ttgggagagc tcccgcagcc cggccagcgc ctctgcgaac gaccctgccc cgcgcagcgc
      121 atcgttcgac gccgggtcga ggccgtccag gctcacgcgc atctcgagcg agtagcggct
      181 ctcccgctcg agggcgcgga ggcggctcgc ctctcttgcg tcgatcaggg tcgcgttcgt
      241 gagcacggtc gagggagcca tcgcgagcgc gtcttccagg atcgaaagga gctcgcgatt
      301 caggaacggc tccccgccgg tgaagtagaa gtcgcgcaca ccgagcgcga gggcttcgtc
      361 caggtagggg cgcgtctccg cgcgcgacat catcgcgtgc cggcgattgg tcgggccgca
      421 cgtgatgaag cagtggctgc acgtgagatt gcacagcgtg cccgccacct ggaaccagac
      481 ggtgtccagg gcgaggagcg gaaccgcggg aacgacggtg atcggaatcg cggcgggcgt
      541 cggcattctc ggagcgtagc acgcgcgggc cgagacgtcc cgccgtgcta tcatgccggg
      601 ccgttccacc cccgttctcc aaatggatcc tcgcatgatc tgggacgtcg tcatcatcgg
      661 tgccggcccc gccggcctct ccgctgcgct cttcacgcgc atgcgccgca tgtccacgct
      721 catgatcgag accgcggtcg caggcggaca gctcgcctcg ctctatccca aaaagccggt
      781 ccacgacatg ccctcctaca cgtacgtgat gggcgaggac ctggggcgga acttcgtcga
      841 gcaggcggcg cacatggggg tcgagatcca cgagggggag cacgtcacct cgatcgctcg
      901 ggacgatgac gcggccgtcg tgcgggtcgt ctcgacgaag ggatcctacg aagcgcggag
      961 cgtcatcgtc gcgaccgggg gcggcgcctt cgagccgcgc aagatcaaca agcctggcga
     1021 agccgagctc aagggacgcg gagtcacgta cggcatgccc gacatcgagg agagccgcgg
     1081 caagcgcgtg ctcgtgatcg ggggcggcga cagcgcgctc gagtcggcgc tctcgctcaa
     1141 ggacgtggct caggtcacgc tcatccacat gctcgacaag tggcagggca tggacggcta
     1201 cgtcgaggac gttcgagagt cctcgatccg ggctctactc gagaccgaga cggtcgagat
     1261 caaggggaat ggccgcgtgg agacggtcgt cgtccggaac aagaagaccg gcgacacgga
     1321 ggagctcccg atcgacctcg tctcgatcaa catcggctat ctcatggaca cgaagatcgt
     1381 gcgccagtgg gggctcgaac tcgaggggag ccagatcaag gtcgatccca tcatgaagac
     1441 gaacatcacc ggggtcttct gctgcggcga catcgccacg taccccggga agtacaagct
     1501 cctcatcacc gcctgcagcg agggggcggc cgcggcgaac acggcctaca tccacgtgaa
     1561 gcagccgaag aagttcaccg tgggcgagct ctacgcggcg ccgaaggagg aaggcgacac
     1621 ccagccgaag gccggggagt aggggcgggg cgtcgaggcg gaacgaacgg agggtcggcg
     1681 cttatccgcc gccgaccgtg agctccttga atcggatgga cggcgagcag acctgggctc
     1741 ggaagtcgag atcccgtccc accgcatcca cgcccatcag catgtcggag atcttcccgg
     1801 ccaccgtgat tccatccacc gggcggccga gctttccctt ctcgatccag ttcccctcga
     1861 cttgctgcga gtagtctccc gccgcgaggt cgaatccgaa tccggccgtg cccgtcacga
     1921 agagaccgtt cggcacctgc tcgatcatcg tcgagagctt ggtcgagccg ttctcgagat
     1981 agaaattgga cggtcccacc gcggggagcg agtcgtagcc gcgccacacg ctccccgacg
     2041 gggtcacgcc ctcgcgccgc gcggtggctg ccgtgtggag gaagcgcgtg agcacgcccc
     2101 gatccgcaac gatgcggcgg ctcgtctggg tcccctcgcc gtcgaagggc cgcgtgccga
     2161 gcccgcggcg gaggatcgcg tcgtccacga gcgtcacgag cggcgagccg atggtctgtc
     2221 ccttcttccc cgcgaggaac gagcgctgct cgagcacgtt gagcccgacg aaagcgccga
     2281 agagcccact caggaatccc cccgcggcct cggcttcgaa gatgacgggc acgcgcgctg
     2341 acggcacggg cttggcgccc aggcgcgcca ccgcgcgctc gcccgccttc cgccccacgc
     2401 ggtccggcgt ggtgaggtcc tggaagttgc gcttccggtc cgtgaaggag ccgacccgct
     2461 tctggcttcc atccgtcgcc acggcggagc aatagagcca gaactgggtg ttgcggaagg
     2521 tttgatacgg gatgaacgtg ttcgagaagt gggtctctcc cacgacgtag ccggcgcgcg
     2581 cggtctgcgt ggtcttcacc ttgttcacga ggagcgcgtt gtcttcgagc gccttcgcga
     2641 gaacgatcag atcttgcggc gtgatgcggt cggcgtaggg atcctcgatc tcgagattcg
     2701 tctgcggcgg gaaggtctgg tccgggagct tgttctcgtc ccgcggcgtc gcctgcgagg
     2761 cgagcgcgat ggtccgcacc accagctcgt ccagcacggt gcggttcagg ttcgtcgtgt
     2821 agaggaagcc catcttccgg tcgcggagca cccggagtcc gagtcccgtg atcgcggact
     2881 gctggagcag ctccacctcg ccctcgcgat accggacctc gacgaagcgg acgaactcga
     2941 agtaggtctc ggcctcggcc gcgccggagc gctccgccat gctgcggacg ttgttcagga
     3001 cttcttcggg gcgggagatg ggcatgctag atcgctgtgc ctccgaccgt gatcgaggcg
     3061 aagcggacgg tgggctgtcc gacgccgacc ggaaccatct ggccgtcctt cccgcacagg
     3121 ccgcgggtct ggtcgacctg gaggtcgctt ccgatgccct cgatccgctg catggtctcg
     3181 agggagttgc cgatcaagac cgcgccgcgc accggcttcg ttctcttgcc gttctcgatc
     3241 atgtagccga gctgcaccgc gaagttgaaa ttgccgctcg tcgtgtccac cgagccgccc
     3301 ccgagatccg cggcgtagaa ccccttcgtg atcccggaga gcagcgacgc cgggtcgtcc
     3361 gtcccccggt cgatgtaggt gttcgtcatg cgcgggagcg gccactgccg gtaggacatg
     3421 cggcgcccgt tccccgtcga cttcagattc aggagggacg cgctctgccg gtcgaggatg
     3481 taggactgga gatagccgcc gtccacgagc accgtccgct ggcccggcgt cccctcatcg
     3541 tcgacggtga aggagccgcg cccgttgggc cagagcgcat cgtcgatcac gcgaaggtga
     3601 ggcgatccga tcttctgtcc catgagtccg ctcaggagcg acgtgccgcg gcggatcgag
     3661 tcgccttcga gcgcgtggcc gaaggactcg tggatgagga cgccgcccca gcccggggcg
     3721 atcacgacgg gcatcgctcc cgcgggagcg ggatccgctc cgagaagcgt caccgcctgc
     3781 agcgccgcat ccttcgcgac cgcctcggga tcgcggcgca ggaaatagtc ctcgccgacg
     3841 cggccgccta ggaagcttcg ccccgtgcgc cggtccggtc cgttcacggc cgtcgcggag
     3901 cagctgatcg agacgaggta ctggcgatcg atcgcgtatc cgccgtccga ggtgccgatg
     3961 agcgtgtcgc gcgcctcgtc ctggtattcg atgcgcaccg acacgacctt cgggctgacg
     4021 ctgcgcgcgg ccgcatccat gcggcgaagg atgtcgaact tcgtgttctc gttcatgagc
     4081 gcgaccgggt tcatgaccga gaacggaggc gtgaggacgc gcggccgcac gtcgaccggc
     4141 gcgacggtgc cttgattgag gacgaccgcg gacgcgacgt gcgccgcgtc gaggagatcg
     4201 ttgtaggcga tctcgtccga gaatgcgtaa ccggtctgcc tccggaagaa gacgcgcacg
     4261 ccggcaccgc ggggataccc gtacgtgacg gaatcgatct tctggtcgtc gaggacaatg
     4321 ttggtgcgga agcgctgctc ggcgaagagg tccgcgaaat ccccgccccg cttgagggcc
     4381 tcgatcagga tctgattgac cgtctcgaga ttgaggacgt cgcggagctc cttgccgatc
     4441 cccatgatcg ccgcgtcggc atcggggggc ttgagccagg agaggccgga cgcgcctgcg
     4501 gccatggcgg cgccggagag gactgcgccg tggcgcagga agtcgcggcg cgagatcggt
     4561 tcgtcgatcg gcatcgggct ggagcctctc agaaaggtcc attggttgca agctcccgcg
     4621 gccgatgcta cgttaggccg cgatggagcg aattcggaag cttttctccg tggatgaggc
     4681 caacctgctc cttcccgagc tggaacgggc gctcgacggg ctcgccgcgc tcggcaagga
     4741 aatcacggcg ctccgccggg agatcgaggt cctcgccgcc atcgaatcga gcggcgcgga
     4801 tgccgccaac cccgacgtcc gcgagctcag ggaccgggag aaggcgtgcg ccgcccgcat
     4861 ggagatcttc cgccgctccc tggaggagat ctcccgccac ggctgcatca tccgggacct
     4921 cgacatgggc ctcgtggact tctacaccgt atccaagggc caggtcgtat gtctttgctg
     4981 gagacgggga gaaccccaga tcgagcactg gcaccccgtg aacgagggct tctccggacg
     5041 tcgtcccctc gacgacatcg cctagcccgg accccgctgc cccaagagcc cgggaggcca
     5101 cccccgtgga caagcaccag gtcgcccgca ccctcgaaga gatcgcaatc ttattggaat
     5161 tgaaggggga gaactccttc aagatccgcg cgcacgagaa cgccgcccgg gccgtcgacg
     5221 ggctggccga ggacctcgcc gtgctcgtcc aggaggggcg gctcacgtcc gtccctggga
     5281 tcggcgactc gatcgcgaag aagatcgccg agctctggac cacgggccgg atgaagcact
     5341 acgacgagct cgcggccgag gtcccccggg ggtatctcga catgatccgg gtgcccgggc
     5401 tcggggcgaa gaagatccgc gcgctcggcg aggcgctcca gatcacctcg ctcgagaggc
     5461 tcaaggaagc gtgcgaggcg ggcgaggtgc gagtcttgaa gggcttcggc gagcagtcgg
     5521 agcggaagat cctccagggc atcgcgctcg tcttgcaagg cgcaggacgc tacctcgcgg
     5581 ccgaagtgcg cccgatggcg gaggagctcc tctcgtacct gcgcggccac gcctcggtga
     5641 aggccgccga ggtgggcgga agcctgcggc gatggatgga gaccgtgaag gacgtggatc
     5701 tcgtcgtcgc cacgaccaag ccggccgcgg tggccaaggc cttcctcgag atccttccca
     5761 tgcacacgat caccggcgcc ggcgacacga agacctccgt ccggcttccg aacggcctcg
     5821 cgatcgacct tcgattggtc tcgccgaagg aattcccctt cgcgctccac tacttcaccg
     5881 gcagcgccgc gcacaacatc cgcgtgcgcg gccgcgcgct cgaccgcgga tggagcctga
     5941 acgagtacac gctcctggcc gaagcggcca aggtgaagaa ggcgccgtcc gtcgcggacg
     6001 aagcggatct cttccgcgcg ctcgggctcg cgtacatcga gcccgagctg cgcgaggatc
     6061 ggggcgagat cgaggcggcg gaggccggaa cgctccccga gctcatcacg ctcgccgaca
     6121 tgaaagggat cctccactgc cacacggtcg cgagcgacgg caagtcgacg ctcgaggaga
     6181 tggcggaggc ggccatcgcg catggcgcgt cctatctcgg gatctcggat cacagcgcct
     6241 ccgcgcgcta cgcgaacggc ctctcggagg aggccttggc cgagcagggg gcggcgatcg
     6301 accgctggaa caagaagtcg aagaagctcc ggatcctgaa agggaccgaa accgacatcc
     6361 ttccggacgg cacgctcgac tatcccgacc acgtgctcgc gaagctcgac ttcgtgatcg
     6421 cctcggtcca cacgcacttc acgatggacg aggccgcgat gacggcgcgg gtggtgcgtg
     6481 cgctgcggaa tccgttcgta tcggtgctgg cgcatccgac ggggcggctt ttgctcca
//
LOCUS       DASTKC010000062        13942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1229070, whole genome shotgun sequence.
ACCESSION   DASTKC010000062 DASTKC010000000
VERSION     DASTKC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13942
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229070"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VFR25_02690"
     CDS             complement(<1..1040)
                     /locus_tag="VFR25_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9939986.1"
                     /translation="MNPIARYTRWLHTRWPAGTVEPLPEARHDGSTSVPGVYVVGDLT
                     GIPLLKFSLDTGVRAVERVAAELGTGRNRTKGLLDLVIIGAGVSGVAAAIEAKRRNLS
                     YKLLEAREPFSTIVDFPKAKPIYTYPRSMKPAGSLQVTATVKEPLVDELREQAKAAGI
                     EAVPFDVERLERASGQILVHGAGGEGLRARRVIVAIGRAGSYHALGVPGEDRDRVYHR
                     LHDPNDFAQADVLVVGGGDTAVETAVALAQCGARVTLAHRKGELTRPKPENRERLERL
                     LHDPNADVTVEHPTDERMSTASGAFAARTHKPGSIRLLLDTQVREIREQSVVLDTKGA
                     AEEIPNDAVFEM"
     gene            complement(1111..1545)
                     /locus_tag="VFR25_02695"
     CDS             complement(1111..1545)
                     /locus_tag="VFR25_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939987.1"
                     /translation="MLEALILSYQSRDSTEYDSLYASSYIGTSTNLNAPPGNQTSTFH
                     LADEAAHIAALAKSTTISSIVLDLGPSSTWTRLPSDDVTHPEWAMIQIGAGNFHGEIS
                     DGGTSWSIQSINPMIFAFTPTVAAPGDTTWKIIRWTEVGQGV"
     gene            complement(1674..2261)
                     /locus_tag="VFR25_02700"
     CDS             complement(1674..2261)
                     /locus_tag="VFR25_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939988.1"
                     /translation="MRMLSALRPFTLLIAAFVVIAGCSDDDEAKPPFQPNPTPAELVS
                     PEITLGLLQQAYEARDSVTTARVYDTDYAGTSTDLNDPPGSQTINFTRANEISHVGAL
                     RKSTTITSVTCDLGPPASWVRLASDDVSHPEWALIHISGGFVRIEINDGASILQAGGP
                     SDQMSFYFTPKPDTTSQTDTLWTVIKWDETRAGVP"
     gene            complement(2271..3569)
                     /locus_tag="VFR25_02705"
     CDS             complement(2271..3569)
                     /locus_tag="VFR25_02705"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HET9939989.1"
                     /translation="MSSPIRHSRGGARRTACALVALAAAFPGEARSAGFAVYEQGVAA
                     TANGGAFTARASDASAVYFNPAALATHEGVEAMVGTTGILLHGGHFTSGATGDHYDQV
                     DNTTFPSYLYLTHRASSRWAWGAGITFPSGLKTEWGPEFEGRFISRMSSLAVMNVNAN
                     VARSIGSGFSIAAGVDVAHVDVKELSRNIDLTPLGFPGSEGFTRMEGTGTGVGWNAAA
                     RWARGAWSAGASYRSKIEPDIDGTIVFENIPAPVAPLFPDGGATAELPLPASLAAGVA
                     HTWGRLWETEADVVWMGWSAFETLNIDVEKETQVGGNPVVRDIVQDESWDDAWSYRVG
                     VARHLGSRHTLRIGGYRDVTPVPADHMRPRIPDSSRSSVQIGYGFASESGILLDAAYQ
                     AIFFDDRTAVGSTSDPTNPVLPGEYGNFIGRIGLSVGYRF"
     gene            3728..4891
                     /locus_tag="VFR25_02710"
     CDS             3728..4891
                     /locus_tag="VFR25_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939990.1"
                     /translation="MPACIVFGANITALAVMRSCGRAGVPVYNAGSDRTTIGRSRWFR
                     PVPGVDVPESASPDRVADLLRALPFDRSVLFPCSDHWALALAALPSDIVTRHTPVTAS
                     LPVVQTLVDKEAFAHAAAAHGVPAPRQFKPQQLSDISDSDLPNFFIKPRDSQLFSGRF
                     GVKAFLLTGRANAEEMLGQLAAERIEVVLQELIPGGATDHVFLDGYVDRRGAIRACFA
                     RRRLRMYPRPFGNSTLSVTIPMREVAAARESLERLYTGLGFVGLFDAEFAFDARDGLF
                     KIVEVNGRPWWQLALSGGSGLDVCVMAYHDALGEPFTVPSEYRIGRTWVHPIPDLSAW
                     WDGVKRGDRTGGFPVRNWFTGPNTVCTWDDPWPVLDELGRLARITFRKVRRTS"
     gene            complement(4922..5626)
                     /locus_tag="VFR25_02715"
     CDS             complement(4922..5626)
                     /locus_tag="VFR25_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9939991.1"
                     /translation="MTQRKWMAFAAAGACFLTAIALAETVATHPGTKMAGALPDTTVV
                     DSIKGAHTVFPRPKADLPAKLREGGYVLVFRHSITDWSQRDADVTNFEDRTTQRNLSA
                     EGEKMAKAIGQSIAALKIPIGRVIASPMWRCRDTAQLAFGRHEVSNDLFQRGSQSRAV
                     RVDLMSTPTEKGKNLVLVTHQDVIIPIVAGLARDQLKEGEALIVRPLGDKKFEVVAQI
                     GPEDWAKLAAADKPTH"
     gene            complement(5623..6459)
                     /locus_tag="VFR25_02720"
     CDS             complement(5623..6459)
                     /locus_tag="VFR25_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939992.1"
                     /translation="MTSRPAWTSRALHWSLRIGVAMCFIGHGAFGIRVKEGWFPFFNA
                     LGFGDEMARRLMPIVGTFDITMGITALLSPCRAVLLWMSVWATFTAFLRPLAGQGFWE
                     VLERAGNYGVPMTFLALSGLGRTWREWFEPIMPSDTPSQHGITREQASRLLRWSTALL
                     LLGHAGYGAFMHKAMLQDHYAVIGIANKAAAVSAVGWFEVALAAGVLFAPIPAFLLFV
                     CAWKVGTELLYPMSGSPFWEWIERGGSYVAPFALFLTQTRSKTAIEPVPRVAPAVEER
                     LA"
     gene            complement(6912..8630)
                     /locus_tag="VFR25_02725"
     CDS             complement(6912..8630)
                     /locus_tag="VFR25_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9939993.1"
                     /translation="MTPEDDDRHERLLKLAQTIADGNPVDWDHALEADPDSTDSIEAL
                     RILESVVAAHVDPPPSIPVPFTWGHLEVREKIGDGAFGEVYRALDTRLRCDVALKLLS
                     SSRAGSKRAADQFIGEARMLAQVRSQHVVTVHGADVHQGRPGLWTDLIRGQTLEQIIT
                     IAGQRSDSEAVTIGVALCQALSAIHKKGLVHRDVKTQNVMREEGSGRIVLMDFGAAAA
                     FLPDELGAREGGESVYGTPLAVAPEVLAGAKATPASDIYSLGVLLYRLVTRRYPVDAS
                     TFRELEERHRTGNRKPLRDARPDLPADFVNVVERALEADPARRYGSVGEMERALLSYG
                     DDRDDRGEKRKPPKWRWPLLAAAALAVLVVAYIAWLSTPPRSSPPIAVQAALAADAKL
                     YRERDGKSERLLPGARVEPGDKLYLELTGDDAMNVYVFNQDEKGSVFVLFPLPGLDAA
                     NPLPAKQSNRLPGSRQGVPESWQVTSAGGSEELFVVASRTPLAALEQELAAVPRARPG
                     APVQLGEEMLHTLRGIGGTEPSEVETAKAPRLSDVLAKVSQDPARSRDLWVWQTRLAN
                     PESRGD"
     gene            complement(8770..11592)
                     /locus_tag="VFR25_02730"
     CDS             complement(8770..11592)
                     /locus_tag="VFR25_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9939994.1"
                     /translation="MSTDRSSTTRLARLLSVESLDDALAEGAALLAEEANVGAAAVFL
                     ADGNQPLRDFWHGSPEARERHRPAFKSAALEATRTIAKSQRPPSGETCELRAFPLAAG
                     GRALGAVCLPWPGDEDDSELEGRIASIAAILAAKAHFHGEIARHQVQRSRDERWFKTL
                     DSHLRVLDRERQKFAAFVGQTDTLVFVTDRDAKISWANRAMCDLFPPEDGGSWIGSSC
                     TSVCARLGGPCAECPVARTSSDAVVSHQELRGKVGSNVEGLLYLTALPICGPNGTPDE
                     IMVMIQDLTGLEGLRRSEMRYRLLYERNVDGILMVRPGTFGITLANPAACSILGFSPD
                     EIKERVLADLHAPDEWDRLSASYDACLAGGSPIALECTLQTRFGEERTAQIAANRFRH
                     EEEEVLLVSFRDVTEMRRAQRALERVEERLRAVIASAPIVLFAIDREGIFTVSEGHGL
                     EALGLKPGEVVGKSVYVLYKDQADILDHVRRALSGEEIEASVEVAGIHFDVRYAPLKD
                     GSGEIQGVIGVATDVTQRRKAEHALHDSEEALRVSEEQLRQAQKMEALGVLAGGVAHD
                     FNNLLTVIMTQSELLQKDLPGGSKEREKAESIHGASARGAMLTRQLLTFSRNEVLAPQ
                     VLDVNTVVGEIEGMLRRLIGEDVEITCSCDTAPVHVRCDRGQLEQVIMNLAVNARDAM
                     PHGGRLRIAVETGEVDEVEAHVLNLDRPGRYCVLRVEDTGSGMDTTTLERIFEPFFTT
                     KGPGKGTGLGLSTVYGVARQNGGSVSVRSEPGRGSTFTVHLPLKEPTAVPTPPKAAPD
                     LRARGGGETVLLVEDEAGVRAIGKDLLEFHGYKVLEAENGVRALEVEARHQGPIHLLL
                     TDVVMPLMGGRELAERLSSRRPETRILFVSGFTDDTVVRHGVLDEGVAFLQKPFTLES
                     LSQTVRQVLDGQPVVPSSPPTQTPKR"
     gene            complement(11589..12062)
                     /locus_tag="VFR25_02735"
     CDS             complement(11589..12062)
                     /locus_tag="VFR25_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9939995.1"
                     /translation="MEIDDLIAHLDEMAAGGAGLERLLETAVEGLSRTDPRFDWTGVY
                     ELFSDNMLRLGPFVGAPTDHVFIGVGNGVCGTAVAERRNMNIPDVREITNYLACSVST
                     RSELVVLIRTGDVIHGQIDIDSHQVGAFDEASVAKVERVADWLARAYDARAGGAS"
     gene            12287..13936
                     /locus_tag="VFR25_02740"
     CDS             12287..13936
                     /locus_tag="VFR25_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9939996.1"
                     /translation="MLRRVSLAFAIAALAAAPAFAQHDHHTTPASGPGAAPLLEGLGQ
                     LHREIKTRAPLAQKYFDQGLRLAYGFNHEEAKRAFLEAARLDSTCAMAWWGAALVLGP
                     NINLPMSPEAETEAYGYAQRALACPPAAPLERGMVEALAKRYDATGGMNRAAHDSAYA
                     DAMRALHKAQPADPDVAVLTAESLMDLRPWDYWAMDGKAQPGTMEIVSILEGVLKSHP
                     NHIGAIHLYIHAVEESPEPGRAEAYADKLAKLAPSAGHLVHMPSHIHLRVGRYADAVD
                     YNAKAISADRAYIGKYNPPGVYPIMYYPHNIHMRWSALLSSGRRADALQAARDLAKAV
                     PDSIVVAMPMAEFFRVAPYLTQARFGLWEEALQEPAPKAGMPFQRGAWHYARGLAFAA
                     GGKYAEAQAERDSVSAVAASLPPDAYFSLNPAAPVYRFAAAHLAGEIALRRGNLDEAI
                     QLLQTAAGMQDSLHYDEPPAWQQTARQSLGTALLAAGRAADAEAVYREDLARYPETGW
                     SLYGLTQALRAEGKTKEAAETQKRFQRAWAKSDTKLVASRF"
BASE COUNT         2194 a         4650 c         4782 g         2316 t
ORIGIN      
        1 atcatctcga acacggcgtc gttcggaatc tcctcggcag cgcccttggt gtcgaggacc
       61 acgctctgct cccggatctc gcgcacctgg gtgtccagga gaagtcggat gctcccgggt
      121 ttgtgggtcc gcgcagcaaa ggccccagag gccgtgctca tacgttcgtc tgtggggtgc
      181 tccacggtga cgtccgcgtt gggatcgtgg aggagccgct cgagccgctc gcggttctcc
      241 ggtttgggtc gcgtgagctc gcccttccgg tgcgcgagtg tgacgcgcgc gccgcattgc
      301 gcgagcgcca ccgctgtctc gacagcggtg tcgccgcccc ccacaaccag cacgtccgcc
      361 tgagcgaaat cattgggatc gtggagccgg tgatagacgc ggtcccgatc ctcgcccgga
      421 acgccgaggg cgtgatagct cccggcacgg ccgatggcca cgatgacgcg acgcgcgcgg
      481 agcccctcgc cgcccgcgcc atgcacgagg atctggccgc tcgcgcgctc gaggcgttcc
      541 acgtcgaagg ggacggcctc gatgccggcc gctttggcct gctcgcgaag ctcatccacg
      601 agcggctcct tcacggtggc cgtcacttgg agggagcccg cgggcttcat cgaacgcggg
      661 taggtgtaga tcggcttggc cttggggaaa tcgacgatag tggagaacgg ctcccgcgct
      721 tccaggagct tgtaggaaag attgcgccgc ttggcttcga tcgccgcggc aacgccggac
      781 acgccggccc cgatgatgac gaggtcgaga aggcccttcg tgcggttgcg ccccgtcccg
      841 agctcagccg caacgcgctc cactgcgcgc acaccggtgt cgagcgagaa cttgaggagc
      901 ggaatgccgg tgaggtcgcc gacgacatac acgccgggca ccgacgtcga gccgtcgtgg
      961 cgtgcctcgg ggagcggctc gaccgtgccc gccggccagc gggtgtgcag ccagcgcgtg
     1021 tagcgggcga tggggttcat ggccgggctg cggtggtggt catcacaagg gagctacgtc
     1081 cggatcgcca cgacgtgacg agggttccgg ctagacgccc tgcccaacct cggtccagcg
     1141 aatgatcttc caggtcgtgt ctccgggcgc ggcgaccgtg ggggtgaagg cgaagatcat
     1201 gggattgatc gactggatcg accacgacgt gccgccgtcg gagatctccc cgtggaagtt
     1261 gccggcgccg atctgaatca tggcccactc gggatgggtc acgtcgtcgg atggaagccg
     1321 ggtccacgtt gacgagggcc cgagatcgag gacgatgctc gagatcgtcg tcgacttggc
     1381 cagagccgca atgtgggccg cctcatcggc aagatgaaag gtcgaggtct gattgccggg
     1441 tggggcgttc agattcgtcg aggtcccgat atagctggag gcgtagaggg agtcgtattc
     1501 cgtgctgtcc cggctctggt acgagaggat cagagcctca agcacgtgtt gcggagaagt
     1561 ccgcgccgga tagttgggtc ccggtggtgg cttcagcggg tccttgccgt cgctgccgca
     1621 cccaccaaga gccgccgccg ctagcacccc cacgacccac cccacgcgcc gcatcagggc
     1681 actcccgcgc gcgtttcgtc ccatttgatc acggtccaaa gcgtatccgt ctgggacgtc
     1741 gtgtcgggct tgggcgtgaa gtagaagctc atctgatccg aaggaccgcc ggcttgcaag
     1801 atcgacgcgc cgtcgttgat ctcgatccga acgaagccgc cggagatgtg gatcagcgcc
     1861 cactccgggt ggctcacgtc atcggatgca agccgcaccc agctcgcggg cgggccgaga
     1921 tcgcaggtga ccgacgtgat cgtcgtggat ttccggagtg cacccacgtg gctgatctcg
     1981 ttcgcgcgcg tgaagttgat ggtttgggag ccggggggat cgttgaggtc ggtggacgtg
     2041 cccgcgtagt cggtgtcgta gacccgcgcg gtcgtcacgg aatcgcgggc ctcgtacgct
     2101 tgctgcagca agccgagcgt gatctcgggg ctcacgagct cggcgggcgt ggggttgggc
     2161 tggaagggag gcttggcctc gtcatcgtcg ctgcatcccg cgatcacgac gaacgccgcg
     2221 atcaggagcg tgaagggccg gagcgcagat agcatcctca tcccccctgc ctagaaccga
     2281 taccccacac tcaagccaat ccgaccgatg aagttgccgt actcgccggg gaggacgggg
     2341 ttcgtgggat cgctcgtgga gccgaccgcg gtgcggtcgt cgaagaaaat ggcctggtac
     2401 gccgcgtcga ggagtatgcc tgattccgac gcgaatccat agccgatctg caccgaggag
     2461 cggctcgaat ccgggatgcg aggacgcatg tgatccgccg gcacgggcgt cacatctcga
     2521 tatccgccga tgcggagcgt gtgccgggac cccaggtgcc gagccactcc cactcggtag
     2581 ctccacgcat cgtcccagct ctcgtcctgg acgatatccc gcaccaccgg attgccgcca
     2641 acctgggtct ccttctcgac gtcgatgttc agggtctcga acgcgctcca tcccatccac
     2701 acgacgtcgg cttcggtctc ccagagcctg ccccacgtgt gcgcgactcc ggccgcgagc
     2761 gatgctggaa gggggagctc ggccgtggcc cctccatccg gaaagagcgg cgccacgggt
     2821 gcggggatgt tctcgaagac gatcgtgccg tcgatgtcgg gctcgatctt cgatcggtac
     2881 gacgcaccgg cggaccatgc gccacgagcc catcgcgcgg cggcgttcca tcccactccg
     2941 gttcccgtcc cttccatccg cgtgaatccc tccgacccgg ggaagccgag cggcgtgaga
     3001 tcgatgtttc gcgacagctc cttcacatcg acatgggcga cgtccacacc ggcggcgatc
     3061 gagaagcccg aaccgatcga cctcgccacg ttagcgttca cgttcatgac cgcgaggctc
     3121 gacatgcggg agatgaatcg cccctcgaat tccgggcccc attcggtctt gagtcccgag
     3181 ggaaacgtga tgccggcgcc ccaggcccat cgcgatgagg cgcggtgcgt caggtagagg
     3241 taggaaggaa acgttgtgtt gtcgacctga tcgtaatgat cgcccgtggc gcccgaggtg
     3301 aaatggccgc cgtggaggag gattccggtc gttccgacca tcgcctccac accctcgtgc
     3361 gtcgcgagcg cagcggggtt gaagtagacc gcgctcgcat cggaggcgcg cgcggtaaag
     3421 gcaccgccgt tcgcggtagc cgccacgccc tgctcgtaga cggcgaagcc cgcgctccgt
     3481 gcttccccgg ggaacgctgc cgcgagggca acgagcgcgc acgcggttcg gcgggcgccg
     3541 ccccgggagt gccgtatggg tgagctcatc ggtcgcacgt acgggagtgt acgtaggccg
     3601 gacggcttgg agaagccttt ccgtggggct gacgccgaca tgccgtggct gctatcctgc
     3661 ccgcgttagg gcgtttccgc gctggccaca ccaccgagtg accaagctcc ccacgacgag
     3721 gagaagtgtg cccgcttgca tcgtcttcgg cgccaacatc acggcgctcg cagtcatgcg
     3781 ctcgtgcggg cgcgccggcg tgcccgtcta caacgcgggc agcgaccgga cgacgatcgg
     3841 acggtcgcgg tggttccggc cggtcccggg cgtggacgtc ccggagagcg cgagccccga
     3901 tcgtgtggcg gacctcctgc gggcgctgcc gttcgaccgc tcggtgttgt ttccgtgcag
     3961 cgaccactgg gccctggcgc tcgccgccct gccctccgat atcgtcaccc gccacacgcc
     4021 ggtcaccgcg tcgctgcccg tcgtgcagac gctggtcgac aaagaggcct tcgctcacgc
     4081 cgccgccgcc catggcgtgc cggcgccccg gcagttcaag ccgcagcaac tatccgacat
     4141 ctccgacagt gatctgccga acttcttcat caagccgcgc gactcgcagc tgttttcggg
     4201 tcgattcggc gtcaaggcgt tcctgctgac gggtcgcgca aacgccgagg agatgctggg
     4261 gcagctcgcg gccgaacgaa tcgaggtcgt gctgcaagag ctgatacccg gcggggcgac
     4321 cgaccacgtg ttcttggacg gctacgtcga tcggcgcggc gcgattcgtg cgtgtttcgc
     4381 ccgccgccgt ctgcggatgt acccgcggcc attcggcaac agcacgctgt ccgtgacgat
     4441 tccgatgcgg gaggttgcgg ccgcgcgcga gtcgctcgag cggctgtata caggactcgg
     4501 gttcgtcggc ctcttcgacg ccgaattcgc gttcgacgcg cgggacggct tgttcaagat
     4561 cgtcgaggtc aacggccgtc cgtggtggca gctcgcactg tccggcggct ccgggctcga
     4621 cgtgtgcgtc atggcctatc acgacgcgct cggcgagccg ttcacggtac cgtccgagta
     4681 ccggatcggc cgcacgtggg tgcatcccat cccggatctg agcgcgtggt gggatggtgt
     4741 gaagcgaggc gatcgcaccg gcgggtttcc ggttcgcaac tggttcaccg gaccgaacac
     4801 cgtgtgcacc tgggacgatc cctggcccgt cctcgatgag ctgggccggc tcgcgcggat
     4861 cacgttccgg aaggtacggc ggacgtcttg agccgggctg agcgagcgcc ttcggcgcgg
     4921 gctagtgcgt gggcttgtcg gctgcagcga gcttggccca gtcctccggg ccgatctgcg
     4981 cgacgacttc gaatttcttg tccccgagcg gcctcacgat caacgcctcg ccttccttga
     5041 gctgatcgcg cgcgagcccc gccacgatgg ggatgatcac gtcctggtgc gtgacgagga
     5101 cgaggttctt ccccttctcg gtgggcgtgc tcatgagatc gacgcgtacg gcgcggctct
     5161 ggctgccccg ctggaagaga tcgttcgaga cttcatgccg gccgaacgcg agctgcgccg
     5221 tgtcgcggca gcgccacatc gggctcgcga tgacgcgtcc gatcgggatc ttgagcgcgg
     5281 cgatcgactg gccgatggct ttcgccatct tctcaccctc ggcgctcagg ttccgctgcg
     5341 tcgtccggtc ttcgaagttc gtgacgtcgg cgtcgcgctg ggaccagtcg gtgatcgagt
     5401 ggcgaaagac gaggacgtag cccccctcgc gaagcttggc cgggaggtcg gccttcgggc
     5461 gcgggaatac cgtgtgcgcc cctttgatgc tgtcgacgac cgtcgtgtcg ggcagggcgc
     5521 ccgccatctt cgtgccgggg tgcgtggcga cggtttccgc gagcgcgatg gccgtcagaa
     5581 agcatgcgcc cgcggccgcg aacgccatcc acttgcgttg ggtcaagcca gccgttcctc
     5641 gacggccgga gccacgcgtg gaacgggctc gatcgctgtc ttgctcctcg tctgggtcaa
     5701 gaagagtgcg aagggagcga cgtagctccc gccccgctcg atccattccc agaatggcga
     5761 cccgctcatc ggatagagaa gctcggttcc caccttccag gcgcacacga agaggaggaa
     5821 cgccggtatg ggcgcgaaga gaacgccggc cgccagcgcg acctcgaacc atccgaccgc
     5881 ggagacggcg gccgccttgt tggcgatgcc gatcacggcg taatggtcct gaagcatcgc
     5941 cttgtgcatg aaggcgccat atcccgcgtg gccgagaagg aggagcgcgg tgctccaccg
     6001 cagcagtctg cttgcctgtt ccctcgtgat tccgtgctgg gacggcgtat cgctgggcat
     6061 gatcggctcg aaccactcgc gccaggtgcg tcccagacca ctcagggcca ggaacgtcat
     6121 cggaacgccg tagtttccgg cgcgctcgag gacttcccag aagccctgcc ccgcaagcgg
     6181 acgcaggaac gcggtgaagg tcgcccagac ggacatccag agaagaacgg cgcggcaggg
     6241 gcttaggagc gcggtgatcc ccatcgtgat atcgaaggtg ccgacgatgg gcatgagacg
     6301 gcgggccatc tcgtccccga aaccgagcgc gttgaagaag gggaaccacc cctccttgac
     6361 ccggatgccg aaggcgccgt ggccgatgaa gcacatggct acgccgatcc ggagcgacca
     6421 gtggagtgcg cgggaagtcc aggcgggacg ggaggtcatg gatgccgagg tcgggtcgat
     6481 ggctggctcg tggttccggc gttcgggcgc gcctccacac gaggcgctcg aacacccaat
     6541 ctagcagaaa tccgctgcaa tttcaagggt ttgggcgggc gggaaggggt ggaacgtgaa
     6601 gcagaggccg gccttggctc aaaccgcgtg gtggcggcgg tgcccgcccc ggcagtgaat
     6661 gaacgccgca cggaacggac catccgtctc cccagccgga tccgccgccc cacgctccat
     6721 gtggcgtgct cgccctattc aaatggctcg ctcgtgtccg gagggacacc agggcggtcg
     6781 gctcgccgaa atccccacag cgtttcggcg ggccccccac ccgttcatgg gggtaaacga
     6841 tgcggcgagg gtcgaacgca cagcaaatct gccgtgggta atcgggagag tgcgttttga
     6901 aaaaacggac gtcagtcgcc ccggctctcc ggattcgcaa ggcgggtctg ccagacccag
     6961 aggtccctgg atcgcgcagg atcttgggag accttggcca gcacgtcgga aagccgcggc
     7021 gccttcgctg tttcgacctc cgacggctcc gtgcctccga tccctcgtag cgtatgcagc
     7081 atctcctcac ccagctggac gggagcgccg ggacgggcgc gaggcacggc cgcgagctcc
     7141 tgctcgagcg cggcaagcgg cgtgcgcgaa gcgactacga agagctcctc gcttccgccg
     7201 gcgctcgtga cttgccagct ctccggcacg ccctgccgcg atccgggaag gcggttgctc
     7261 tgcttggccg gtaagggatt ggccgcatcg agaccgggaa gcgggaagag cacgaagacg
     7321 cttcccttct cgtcctgatt gaagacatag acgttcatcg cgtcgtcgcc ggtgagctcg
     7381 aggtagagct tgtccccggg ctccacgcgc gcgccgggca ggagccgctc cgactttcca
     7441 tcgcgctccc gatagagctt ggcgtccgcg gcgagcgcgg cttgcactgc gatcggaggg
     7501 gatgaacgtg gcggggtgga cagccaggcg atgtaggcca cgacgagcac ggcaagcgcc
     7561 gccgccgcga ggagcggcca gcgccacttc ggcggcttcc gcttctcacc gcggtcgtcg
     7621 cggtcgtcgc cgtagctgag gagcgcacgc tccatctctc ccacgctccc gtaacggcgg
     7681 gccggatcgg cctcgagggc gcgctccacg acgttcacga aatcggccgg aaggtcgggc
     7741 ctcgcgtccc ggagcggctt tcggttcccc gtccggtgac gctcttcgag ctcgcggaat
     7801 gtggatgcgt cgacgggata gcggcgggtg acgaggcggt agaggagaac accgagggag
     7861 tagatgtcgg aagccggcgt ggccttggcg ccggccagaa cctccggcgc tacggcgagc
     7921 ggcgtgccgt agaccgactc cccgccttcc cgcgcaccca gctcgtcggg taggaaggcg
     7981 gcggccgcgc cgaagtccat gaggacgatg cgaccgctcc cttcctcgcg catgacgttc
     8041 tgggtcttca cgtcgcggtg cacgaggccc ttcttgtgga tggcggagag cgcctggcag
     8101 agcgcgacgc cgatggtcac cgcctcgctg tcgcttctct ggcccgcgat ggtgatgatc
     8161 tgctcgagcg tctggccgcg tatgaggtcg gtccagagtc cggggcgccc ctggtggaca
     8221 tcggcgccgt ggaccgtgac gacgtgctgg gaacgaacct gggcgagcat tctggcctcg
     8281 ccgatgaatt gatcggcggc ccgcttcgat ccggcccggc tcgatgagag gagcttgagc
     8341 gcgacatcac aacgcaggcg cgtgtcgagc gcgcggtaca cctcaccgaa ggcgccgtcg
     8401 ccgatcttct cccgcacttc caggtgcccc catgtgaacg ggaccgggat gctgggggga
     8461 ggatcgacgt gggccgccac gaccgattcc aggattcgga gcgcctcgat gctatcggtg
     8521 ctgtccgggt cggcttcgag cgcgtggtcc caatcgaccg gattgccgtc cgcaatcgtc
     8581 tgggcgagct tcagaagccg ctcgtgccgg tcgtcgtctt caggagtcac gctctgcctc
     8641 cttggattcc cgccgcaccg tgggggcggt gccctcatcg ttccatgact agaacgccgg
     8701 aatcggcttc atctgcacag gataatgaag cggggagggg cccgccagcg ggccgaagac
     8761 gggcctaagt cagcgtttgg gggtctgggt aggtggagac gagggcacga ccggctgtcc
     8821 gtccaggacc tggcggacgg tctgggaaag gctttccagc gtgaacggct tctgcaggaa
     8881 ggcgaccccc tcgtccagca cgccgtggcg gaccaccgtg tcgtccgtga atcccgagac
     8941 gaagagtatg cgggtctcgg gccggcgcga gctcagccgc tccgccagct cccggccccc
     9001 catgaggggc atgacgacgt cggtgagaag aaggtggatc ggtccctggt gccgggcttc
     9061 gacctcgagg gcgcgcaccc cgttctccgc ctcgagcacc ttgtagccat ggaattccag
     9121 gagatccttg ccgatcgcgc gcaccccggc ctcgtcctcg acgaggagga cggtttctcc
     9181 tcccccccgc gcgcggagat ccggcgccgc cttcggtggg gtgggaacgg cggtgggctc
     9241 cttgagcgga aggtgcacgg tgaacgtcga gcctcgcccc ggctcgcttc gaacgctcac
     9301 gctgccgccg ttctgccgcg cgaccccata cacagtggag agtccgagcc cggttccctt
     9361 tcccggcccc ttcgtcgtga agaagggctc gaagatccgc tcgagcgtcg tcgtgtccat
     9421 tccggagccg gtgtcctcga cgcgaagcac gcaatagcgt ccggggcggt cgaggttgag
     9481 cacgtgcgcc tccacctcgt cgacctcgcc cgtctccacc gcgatgcgaa gccgtccgcc
     9541 gtggggcatc gcgtcgcgcg cgttcaccgc gagattcatg atgacctgct cgagctgccc
     9601 gcggtcgcag cggacgtgga ccggcgccgt atcgcaggag caggtgatct ccacatcctc
     9661 tccgatcagg cgtcggagca tgccttcgat ctcgcccacc acggtgttca cgtcgagcac
     9721 ttggggcgcg agcacctcgt tccggctgaa ggtgaggagc tgccgcgtga gcatcgcgcc
     9781 cctcgcgctc gcgccgtgga tcgactcggc cttctcgcgc tccttcgatc cgccggggag
     9841 atccttctgg agcagctcgc tctgcgtcat gatcacggtg agcaggttgt tgaagtcgtg
     9901 ggccacgccc cccgcgagaa cgccgagggc ctccatcttc tgggcctgac gcagctgctc
     9961 ctcgctgacc cggagcgcct cctcggaatc gtggagcgca tgctcggcct tccgcctctg
    10021 agtcacgtcc gtcgcgacgc cgatcacgcc ctggatctcc ccgctcccgt ccttgagcgg
    10081 cgcgtagcgg acgtcgaagt ggatgcccgc cacctccacg ctcgcttcga tctcctcccc
    10141 cgagagcgcg cgtcgcacgt ggtcgaggat gtccgcctgg tccttgtaga gcacgtacac
    10201 ggactttccc accacctcgc ccggcttcaa accgagcgcc tcgagcccat gcccttcgga
    10261 gacggtgaag attccctcgc ggtcgatggc aaagaggacg atcggagcgg atgcgatcac
    10321 ggcccggagg cgctcctcca cccgctcgag ggcgcgctgg gcacggcgca tctcggtgac
    10381 gtcccggaag ctcacgagaa ggacttcttc ctcttcgtgc cggaagcggt tggccgcgat
    10441 ctgcgccgtc cgttcctcgc caaagcgcgt ctggagcgtg cactcgagcg cgatgggcga
    10501 tccgcccgcg aggcacgcgt cgtaggacgc gctcaggcgg tcccactcat ccggcgcgtg
    10561 gaggtcggcg agcacgcgct ccttgatctc gtcgggcgag aaaccgagga tcgagcacgc
    10621 cgccggattg gcgagcgtga tgccgaacgt gccgggacgc accatcagga ttccgtccac
    10681 gttccgctcg tagaggagcc ggtagcgcat ctccgaccgg cgaagtccct cgagtccggt
    10741 caggtcctgg atcatcacca tgatctcgtc gggcgttccg ttcggcccgc agatcggaag
    10801 cgcggtgaga tagaggaggc cttcgacgtt gctcccgacc tttccgcgaa gctcctgatg
    10861 gctcacgacg gcatcgctcg acgtgcgcgc gaccggacat tcggcacagg gcccgccgag
    10921 tcgcgcgcac accgacgtgc agctcgatcc gatccaggat ccgccgtcct cgggcgggaa
    10981 gaggtcgcac atggcgcggt ttgcccagct gatcttcgcg tcgcggtccg tcacgaagac
    11041 gagggtgtcg gtctgtccga cgaacgcggc gaacttctgc cgctcccggt cgagcacgcg
    11101 aagatggctg tcgagggtct tgaaccagcg ctcgtcgcgc gagcgctgga cctggtggcg
    11161 ggcgatctcg ccgtggaagt gcgccttcgc ggcaaggatc gcagcgatcg acgcgatccg
    11221 gccctcgagc tcggaatcgt cttcgtcgcc cggccagggc aggcacacgg ctccgagtgc
    11281 gcggccgccg gcggccagcg gaaacgcgcg aagctcgcag gtctcaccgc taggcgggcg
    11341 ctgcgacttg gctatggtcc gggtggcctc gagcgccgcg ctcttgaagg ccgggcgatg
    11401 acgctcgcgc gcttcggggg agccgtgcca gaaatcgcga agcggttggt tcccgtcggc
    11461 gaggaacacc gcggccgcgc ccacgttggc ttcctccgcg aggagcgctg cgccctccgc
    11521 gagcgcgtcg tcgagactct cgacgctgag aaggcgcgcc agccttgtcg tggaggagcg
    11581 gtcggtgctc atgatgctcc gccggcgcgc gcgtcgtagg cgcgggcgag ccagtcggcc
    11641 acgcgctcga ccttggccac cgaggcctcg tcgaacgcgc cgacctggtg gctatcgatg
    11701 tcgatctgac cgtgaatgac gtcgccggtc cggatcagga ccacgagctc ggagcgcgtg
    11761 gagacgctgc acgcgaggta gttcgtgatc tcgcggacgt cgggaatgtt catgttccgg
    11821 cgctcggcga ccgccgtgcc gcacacgccg ttcccaaccc cgatgaagac gtggtcggtg
    11881 ggcgcgccga caaaggggcc gagcctaagc atgttgtcgg agaagagctc gtagacgccg
    11941 gtccagtcga acctcggatc ggtgcggctc aagccttcaa ccgctgtttc gagaaggcgt
    12001 tcgagccccg ccccgcccgc ggccatctcg tccaggtggg cgatcagatc atcgatttcc
    12061 atcagccaat gatcggaaga atgaggaccg ctctttagcg tcgggcgggg gtctccggag
    12121 caggtaagtg gatgacagta agccgattat ccgatgcgac ccgccgaaag gcgggtcacg
    12181 gaaccgggag tgggccgtac gggtggatga cgttgtggct gggcgggagt aggttacgtt
    12241 cgcccgtcga tggatttgcg ccctgaaacc ctcggaggaa ccctcgatgc tgcgccgcgt
    12301 ctccctcgcg ttcgcaatcg ccgccttggc cgccgcaccc gccttcgccc agcacgatca
    12361 ccacacgact ccagcatccg gcccgggcgc cgcaccgctc ctcgaagggc tcggccagct
    12421 ccatcgcgag atcaagacgc gcgctccgct cgcgcagaag tatttcgacc aggggctaag
    12481 gctcgcctac ggcttcaacc acgaggaggc caagcgggcg ttcctcgagg cggcgcgtct
    12541 cgactccacg tgcgccatgg cgtggtgggg cgcggcgctc gtgctcggcc cgaacatcaa
    12601 cctgcccatg agcccggagg cggaaacgga ggcctacggc tacgcgcagc gcgcgctcgc
    12661 ctgcccgccg gcggcgccgc tcgagcgcgg gatggtcgag gcgcttgcga agcgctacga
    12721 cgcgaccggc gggatgaatc gcgctgcgca cgattccgcc tatgccgacg cgatgcgcgc
    12781 gctccacaag gcgcagcccg cggatcccga cgtcgcggtc ctcacggccg agtcgctcat
    12841 ggatctccgc ccctgggact actgggccat ggacggcaag gcgcagccgg gcacgatgga
    12901 gatcgtgtcg atcctcgagg gagtcttgaa gtcgcacccg aatcacatcg gggcgattca
    12961 tctctacatc cacgccgtcg aggaatcgcc ggagccggga cgcgccgagg cctatgcgga
    13021 caagctggcc aagctcgcgc cgagcgcggg gcacctggtc cacatgccga gccatatcca
    13081 tctgcgcgtg ggacgctacg cggatgccgt cgactacaac gccaaggcga tctcggcgga
    13141 tcgcgcgtac atcgggaaat acaacccgcc cggcgtgtat ccgatcatgt actacccgca
    13201 caacatccac atgcgctggt cggcgctcct ctcttccggg cgccgcgccg acgcgctcca
    13261 ggcagcgcgc gatctcgcga aggccgttcc ggactcgatc gtcgtggcga tgccgatggc
    13321 ggagttcttc cgcgtcgcgc cgtatctcac gcaggctcgg ttcgggctct gggaggaggc
    13381 gctgcaagag ccggcgccca aagcggggat gcccttccag cgcggcgcgt ggcactacgc
    13441 gcgcggcctc gcgttcgccg ccggaggcaa gtatgcggag gctcaggccg agcgggacag
    13501 cgtgtccgcc gtcgcggctt cgcttccgcc ggacgcatac ttcagcctga atccggccgc
    13561 gcccgtctac cgattcgcgg ccgcacatct cgcgggtgag atcgcgctcc gccgcgggaa
    13621 cctggacgag gccatccagc tcctccagac cgcggccgga atgcaggact cgctccacta
    13681 cgacgagccg ccggcgtggc aacagacggc ccgccagtcc ctcggcacgg cgctcctcgc
    13741 ggccggacgc gcggcggacg cggaagcggt gtatcgtgag gatctcgcgc gctacccgga
    13801 aacggggtgg tcgctctatg gactcacgca ggcgctccgc gcggaaggaa agacgaagga
    13861 agccgccgag acccagaagc gcttccagcg cgcctgggcg aagtcggaca cgaagctcgt
    13921 ggcttcacgg ttctgagctg aa
//
LOCUS       DASTKC010000063        24493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_283184, whole genome shotgun sequence.
ACCESSION   DASTKC010000063 DASTKC010000000
VERSION     DASTKC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 24493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24493
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283184"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFR25_02745"
     CDS             <1..588
                     /locus_tag="VFR25_02745"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9939997.1"
                     /translation="ERVVKRAGIVYHRAEGEAVFYGPKIDIKISDALGRAWQCSTVQF
                     DFNLPRRFEVNYVGSDGQEHQVYMIHRALLGSLERFFATLIEHYGGDFPVWIAPVQVQ
                     LITIADAQIPYAEEIKAMLLDRGVRVEADLREEKMGAKIRDAELLKIPYMLVLGRQEV
                     ENRAVSVRSRKKGNEGSVPLDRFVENLVAEVEART"
     gene            810..1415
                     /gene="infC"
                     /locus_tag="VFR25_02750"
     CDS             810..1415
                     /gene="infC"
                     /locus_tag="VFR25_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9939998.1"
                     /translation="MRVNDRIRIPSVRVIGPDGEQVGILSIREALAYAQERQLDLVEV
                     SPTAKPPVCRVMDFGKFKYEQNKKQQKARRKQHVTHLKEVKLRPKIEEHDYNFKVEHG
                     RRFLELHDKVKFTVMFRGREMAHPEAGTRLLEKVIKDLELVGQVEIPARMEGRSMVLL
                     MVPRPTTAAAKPAEAKKPAPAGAPAPQPAAPRAEAAKTAKP"
     gene            1426..1626
                     /gene="rpmI"
                     /locus_tag="VFR25_02755"
     CDS             1426..1626
                     /gene="rpmI"
                     /locus_tag="VFR25_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET9939999.1"
                     /translation="MPKMKTNRAAAKRFRRTGTGKLKRAHAFARHHLGLKSRKRKRKL
                     HASAITESTERRHLQKCLPYGG"
     gene            1667..2029
                     /gene="rplT"
                     /locus_tag="VFR25_02760"
     CDS             1667..2029
                     /gene="rplT"
                     /locus_tag="VFR25_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9940000.1"
                     /translation="MPRTKTVVPGRKRRRKVMTAVKGNRGGRRKLYQTARETLMRSLQ
                     FAYRDRRAKKREFRKLWIVRINAAARHHGLSYNAFIRGLKAASVEIDRKVLADLAVRD
                     GEAFARLAEVAKQATIAA"
     gene            2046..3062
                     /gene="pheS"
                     /locus_tag="VFR25_02765"
     CDS             2046..3062
                     /gene="pheS"
                     /locus_tag="VFR25_02765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9940001.1"
                     /translation="MIERLDALRAEGERALADAASPADLEAARVRFLGRKSELTGILR
                     GLKDLTPDERTLVGARANQTRARLEALLAEAESRLQAASAPASARDVTLPGRRPPVGH
                     RHLIPSVLRELEGIFRRMGFAVAAGPDVEDDWHSFGALNMPKGHPARAATDTFYLEGD
                     SIVLRPHTSSVQIRAMGASKPPLRIISPGRVYRNEAVDATHLFEFHQIEGLYVDENVS
                     MADLKGTLDRFLKEFFGPKTEMRFSPSYYPFVEPGAVVDIRCFFCGGKGCTVCGPLSG
                     GWLELLGAGMVHPNVFREVGYDPERWTGFAFGVGVERLIMLRHGVPDSRYLFGNDIRF
                     LTQF"
     gene            3070..5499
                     /gene="pheT"
                     /locus_tag="VFR25_02770"
     CDS             3070..5499
                     /gene="pheT"
                     /locus_tag="VFR25_02770"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9940002.1"
                     /translation="MKVTWNWLGDWVDLAGTPEELAQLLASRGLPVESVERGVTFDPA
                     IVVGHVVEKGQHPNADRLSLCSVDIGGPARLSIVCGAPNVAAGQKVAVAQIGSKLPDG
                     TKLRKSKIRGVESEGMICSERELGLSDESQGIWVLPGDPPIGAPLASVVGGGDPVLDV
                     EITSNRPDCMSVIGLAREIAAARGKKLKPPRPLAAAGSGALPPVTIENPADCPRYMAR
                     VVRGVKIGPSPDWLKKRLEATGFRSISNVVDATNYVLREHGQPIHAFDAAKIGGFAIV
                     VRRAKPGEKLTLLDGREMALDARHLVIADRSIPMALAGVMGGLDSGVTDTTVDVVLES
                     AVFDAALTQATAKSLGIATDASARFIEGVDPVGVERALDTTARLLAEIAGGTVTRESV
                     DQWPGRREHAPISLSRRKVERLLGIPLDPAAITSALSTLEIEPAGSWAAKEGDEVAPF
                     RAPSYRHDLAIQEDLIEEVGRAVGYDAIPLRLVAAPSGPDLVRTDALVAERLLDLATG
                     LGFQEALTNILVGDIPADAREGIGDDQIWELKNPMSRELKHLRVGILPGLVEAAGRNL
                     RMGAADVRLAEAGKVFLSSPPPLGTERYEAGLILAGTPDEWDHPDADTDRYLELKGAV
                     EALLQGFGIDSCRTATYHGRNWSEGSGASMRVGSETVAQMGEVAPSLKASMGLDRPAW
                     AAILDVAAIERVAPAVRRYRDIPRYPATKRDLAVIVPRQTHHAELEDAIRKAGGALLD
                     RVRLFDVFEGRSIGEGKKSMAYALEFRSPDRTLSDREADGAVQSIVGALEARFGAVLR
                     GGPSGRTGDLT"
     gene            5496..5783
                     /locus_tag="VFR25_02775"
     CDS             5496..5783
                     /locus_tag="VFR25_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940003.1"
                     /translation="MMAELDQLEDRIHAAVGLLEKYREEKRRLESENGELKDRVHALE
                     VAAGKQAEDLKPKIQALEDERTALLDERRVVARRVEEMMSKLDQLHKAVHA"
     gene            5854..6147
                     /locus_tag="VFR25_02780"
     CDS             5854..6147
                     /locus_tag="VFR25_02780"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET9940004.1"
                     /translation="MTTEPNTQSVTILGSEYKIRGADPDYIQVVAAYVDGKMRELEGR
                     IPPGTPAKLAILTSLNIADELFREREERTRRDNELRERTKEWGRLLMEVTRES"
     gene            6219..6400
                     /gene="ssrS"
                     /locus_tag="VFR25_02785"
     ncRNA           6219..6400
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFR25_02785"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6424..7992
                     /gene="rny"
                     /locus_tag="VFR25_02790"
     CDS             6424..7992
                     /gene="rny"
                     /locus_tag="VFR25_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET9940005.1"
                     /translation="MTLAPWLIILIALGAALVAFFGGWIAHRRVGEGKLLSAEELAKK
                     VVREAERESENLKKGALLEAKDEWFREKAKLDREVQGQKAEVQKAERTLHELESNLNR
                     RAEVLDKKERDQKRTDRELEVKGEALAERDKDLARILNEQNVRLQRISGMTMDEAKSQ
                     LIANMENEARADAARKLQEIREDTIRNAEKEAREIITLAIQRTAADHSAETTVSVVHL
                     PNDEMKGRIIGREGRNIRAFETATGIDVIIDDTPEAVILSGFDPVRREIAKRSLTRLV
                     TDGRIHPARIEEIVAKVQKEVEDQILELGEGAVLEVGVHSMHPEIVKLLGRLQYRTSY
                     GQNILQHSKEVAWICGIMAAQLGFDVQLAKRAGLLHDLGKAVTHEVEGAHAQISMDFA
                     KKYGEPEEVVKAVGYHHHDPTAENLYAVLVMAADAVSGARPGARRESIENYVKRLEAL
                     EKIADSFPGVEKSYAIQAGREVRIMVEPKNVDDSRAATLASDVARRVERELQYPGMIK
                     VVVIRETRAMEYAK"
     gene            8015..8824
                     /locus_tag="VFR25_02795"
     CDS             8015..8824
                     /locus_tag="VFR25_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET9940006.1"
                     /translation="MNILFVADVYGSPGRRAVRELLPDIVSSMGIDFTVVNVENAAAG
                     FGVTKDTLEEMKTLGVDAMTSGNHIWDRRESLPLLDSEPLLLRPHNYPPGVPGTGSRV
                     FTSRDGVKVGVLNMMGRVFMRELLCPFRTADREIERLKDEGARVVLIDFHAEATAEKV
                     ALGWYVDGRASAVLGTHTHIQTADDRILPGGTGYITDCGMTGPFDSVIGVKKEQAIQK
                     FLTLLPTRFEPAGGDVRLNGVHLDIDETTGLCRHIERIQLALEPAVRPATA"
     gene            8833..9717
                     /locus_tag="VFR25_02800"
     CDS             8833..9717
                     /locus_tag="VFR25_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET9940007.1"
                     /translation="MTAVRLAGGPLADEIKASVRGRFERLRAKGIVPGLAVVTVGEPP
                     GGNPYVRSKCRAAEELGAQAHVTRLPHTASEAEVRATLQDLSDHPAYHGIILQLPLPS
                     SLHEERHLEDIVPVKDVDGIHPMNVGRWVSGLPAHRPATPAGIVELLRRHVGDLSGKR
                     ICIVGRSRIVGRPLSVFLSERATGMNSTVTLCHSASRDLASITREAEILVVAMGHPRA
                     IGAAHVRPGAIVVDVGIHAIENPAPGGPKYEGDVDYEAVSKIASAVTPVPGGVGPLTV
                     AVLLRNLADAAEAAAERA"
     gene            9714..11114
                     /gene="xseA"
                     /locus_tag="VFR25_02805"
     CDS             9714..11114
                     /gene="xseA"
                     /locus_tag="VFR25_02805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9940008.1"
                     /translation="MSPRIGAAEIDFHQGQPRVYTVHEIAQAINLTLGDHGVVSVQGE
                     ISSFRRQSSGHIYFELKDDNARLRVSWFRNRIRQSIDALGDGVQVQVDGRLEFYATGG
                     TVSLIADRVSPVGLGALQARFDALKRALEAEGLFRQERKRPLPAYPTRIGIVTSPSGA
                     AVRDMLRILRQRAPYVRVVVAPSSVQGAGAAFEIAAAIALLNDWGKVDVIIAGRGGGS
                     IEDLWAFNEEAVVRAIAGSRIPVISAVGHETDVTLADLVADLRAMTPTHAAQVVAPSR
                     EDVCATLDKVAKHARDRLGRELREARAHLAGIRNHHALREPGHMIRNNRRRVDELSDC
                     LGRGLGDWVLKRRRRVDQLTGVLHAHTPTRTLERSRERLTALADRARRSTLSQVQRDR
                     TAVSGAGRLLASYDHRGVLRRGYALVWSPDRKLIARGGTLRPGDDVQVEFADAKAEAK
                     VARVDRKPESSEGGAT"
     gene            11111..11410
                     /locus_tag="VFR25_02810"
     CDS             11111..11410
                     /locus_tag="VFR25_02810"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET9940009.1"
                     /translation="MTPKANAPAPDDAGAITFEQMMGRLQDLVDKLEEGQLSLDESIR
                     SFEEGMALVKKCTEVLDRAEERIQKLTRDSSGRPMTSPLDGDEDEAGTGGDELPF"
     gene            11388..12284
                     /locus_tag="VFR25_02815"
     CDS             11388..12284
                     /locus_tag="VFR25_02815"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET9940010.1"
                     /translation="MATSSRFERYLERARPKIEAALDRLLPSASREPKTLHRAMRYSV
                     FAGGKRLRPALCLLACESISGKSTPALPAAAALEMIHTFSLIHDDLPGMDDDDLRRGR
                     PTNHKVFGEGMAILAGDALLSLAFETLSRSRNLKRGANPCALLETVCMATGPEGMIGG
                     QAMDLLFEGRKVPLRSVYAMHRRKTGALLHGSLRLGAQIGGASAAELRAFQAFGERVG
                     LAFQIGDDLLNVRSTRKELGKATGSDRARGKATVPGRVGAERAEREAARLLREAAAIA
                     PKLGRRPEEFVSMTEFLLHRTH"
     gene            12286..12732
                     /locus_tag="VFR25_02820"
     CDS             12286..12732
                     /locus_tag="VFR25_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HET9940011.1"
                     /translation="MPISSSPLVHALACGLLVQLSKVLTFLLREKKINVRRLVETGGM
                     PSSHAASVCALSTCVGLREGIGSVLFQVVLFFSLIVMYDAAGLRRAAGRQATLLNRIL
                     HEHIQLPGPPHERLRELLGHTPIEVLVGAVIGILFSLAIYRSPGGM"
     gene            12729..14660
                     /gene="dxs"
                     /locus_tag="VFR25_02825"
     CDS             12729..14660
                     /gene="dxs"
                     /locus_tag="VFR25_02825"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET9940012.1"
                     /translation="MSTDYLSQVHGPDDLKRLPTDALPALAAEMRERIIQVVSRTGGH
                     LAPSLGTVELTIALHYVYDTPRDVLIWDVGHQAYGHKILTGRNERFDTLRQEAGLSGF
                     PRRVESEFDPFGTAHASTSISAALGFACARDLRGDKNRVIAVVGDGALTGGLAYEGLN
                     QAGVLGSDILVVLNDNSMSISPNVGAIARYLTRLSSSPTYRRFESDVWDLLGKLPAGR
                     KARRLVGRVKEGMKNLMVPNILFEELGFKYYGPVDGHDLGELVELLRQLKDVKGPVLL
                     HALTRKGKGYKFSEDDSRKFHGVASFDKLTGASAKKAGGTSPTYMEIYGQTMIDLASA
                     DRSIVAITAAMTDGTGLAKYATTLPAQFHDVGIAEQHGTCFAAGLAAAGMKPFVTIYS
                     TFLQRAYDQIVHDVVVQNLPVRFALDRAGFVGEDGATHHGLFDIAYLRCLPNVVLMAP
                     KDENELRHMLATMASYSAGPIAVRYPRGSGPGAALDPKLTPLPIGEGELLRNGGDLLL
                     VAYGTMVPAAEKAAQLLEARGIKAAIINARFAKPLDERLILEWARRVPRVATLEEGAL
                     PGGFGDAVLEFLATQSIDGLRLRRFGVPDRLFDHATRDALLRAAGLDPETLAAELERF
                     VRGESRSTPEPIAPAVTRA"
     gene            14657..15535
                     /locus_tag="VFR25_02830"
     CDS             14657..15535
                     /locus_tag="VFR25_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET9940013.1"
                     /translation="MTRPPRHVGIFGNRDKAEVRALVPELARWIQKQGIKVSAESGIT
                     RGRGLSLASLVKKVDVLLVLGGDGTMLHAARAASQAGVPIFGINLGGLGFLTETSDDE
                     LYPALERLFRGDVHTERRLMVEARVKSRSGRTWSAVGLNDAVIHARDRSRVVAIDVRV
                     GPTPIGTLVADGLIVATPTGSTAYSLSAGGPIVGPTIEALIATPISPHTFAFRPLLVP
                     VGETVVARVRAGHAPAAVTVDGQQSRPLSFDDEVTFRRAKAHVDLLVLERGTFYEVLR
                     TKLAWAELPRTRAARR"
     gene            15540..17264
                     /gene="recN"
                     /locus_tag="VFR25_02835"
     CDS             15540..17264
                     /gene="recN"
                     /locus_tag="VFR25_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET9940014.1"
                     /translation="MLSWLSIQGLALVDSVELSLDPGLNILTGETGAGKSLVLGSIGL
                     LLGERADASWLRAGETRGSVEAVFDLGGRPDLLASLAELGWETEEGRLLLRREIQAEG
                     KGRAFVNGRAALLAQLKAIGERLVDLHGQHEHQQLLDPARQTDFFDGWAGLLGERRAL
                     EEERASIVEERAALRAARERWEREREAEGTDREDFEELERASLDPDEEQKLKEDRERL
                     VHRERLLRALGEARRAAADDEDGAVAAVGRAVRALRGAAALDATVQDVASEAERLQGE
                     LAELGGRLEREEERLVDGVLDPEALEARLDLIHRLKRRHRTDLPGLVALREELRAKVR
                     AFDPSGRELDKAERSHDERLQAFERRLDAFLAHRNDRYAAFESDVGARLGTLGFNRSA
                     LRVRPADTGRAVVDPAPIPHLEFAFQPNPGEGERPLRKIASGGELSRVMLAVKSLMAE
                     RDQVAVLVFDEVDQGIGGAVGEEVGRLLEAIGQRRQVLCITHLPLIAAHGARHFEVAK
                     SVQAKRTRVSVRALEPKERVEELARLLAGDRATETTRTQARELLVAAASGGRETKKEP
                     RRAATRRA"
     gene            17261..18736
                     /gene="trpE"
                     /locus_tag="VFR25_02840"
     CDS             17261..18736
                     /gene="trpE"
                     /locus_tag="VFR25_02840"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET9940015.1"
                     /translation="MIRPSLDDYRRLAREHNLIPVTREILADFDTPVSAFVKLNRGDA
                     SFLLESLEGGETWGRYSILGFRPSVEFRSSGTKVEIRRGERTERMEREDPLAALQDLI
                     ADFRAAPVPDLPPFSGGAVGMVGYDYVRFLERLPSRLTADRDYPDLQFVFPDVVLVFD
                     NFRHRLQVVVNSRPGSAPDRAYAEAGVVIEEMIARLAAPMPNPAPVPHPNGDVRFRST
                     MGEDAYKKAVVKAKEHIRAGDVVQVVLAHHLEADVPVAPLDVYRALRVLNPSPYMFYL
                     RFADRSVAGSSPEILVRVHGDRVTLRPIAGTRRRGATRAEDTALEHELKESEKDRAEH
                     VMLVDLGRNDVGRIAAIGSVRTTELLTLERYSHVMHLVSHVEGRLRNGLGAFDVLRAC
                     FPAGTVSGAPKKRAMEIIESLETTRRGAYAGAMGYIDFQGNADFCITIRTAIIDGGRV
                     RCGVGAGIVADSDPDEEWTETCNKAKAVEEAVRLAARGLET"
     gene            18733..19323
                     /locus_tag="VFR25_02845"
     CDS             18733..19323
                     /locus_tag="VFR25_02845"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET9940016.1"
                     /translation="MILMIDNYDSFTWNLVQYLGELGDTPIVKRNDAITPDEIAAAKP
                     RAIVISPGPGRPDDAGVSKDVIRRFSGTIPILGVCLGHQCIGEVFGGRVVQAERLVHG
                     KTSAIIHTGRGLFLDVDNPFQATRYHSLIVAREGLPDTLQVMAWTPENEIMALRHREH
                     ETWGVQFHPESILTTSGKDILRNFLLSLPAPATAAR"
     gene            19320..20342
                     /gene="trpD"
                     /locus_tag="VFR25_02850"
     CDS             19320..20342
                     /gene="trpD"
                     /locus_tag="VFR25_02850"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9940017.1"
                     /translation="MIRQLLPRLVRGERLAREETAAAIRSIMRGGEDPELVGAFLMAL
                     AQRGETAEELAGGAEALRAEAVPFPGEGPFLDTCGTGGDGTHTYNISTVVGLIAAAAG
                     QRVAKHGNRSVSSRCGSADVLEALGARVDLGPESAKRALEETGFCFLYAPRFHPSMKQ
                     VAGIRRSLGVRTLFNWVGPLANPARATHQLLGVSDRARVDVVAQVLAALGAERALVVH
                     GAGGLDELSLEHGNHAVWVERGREPRGVTVEADAIGLPPASVAELEGGTPEENAGHVR
                     AVLSGRAGARRDAAILNAAAALWVAGAADSLEEAAVLATEALDSGAAARVLDRYAAFS
                     QEIGAD"
     gene            20344..21147
                     /locus_tag="VFR25_02855"
     CDS             20344..21147
                     /locus_tag="VFR25_02855"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET9940018.1"
                     /translation="MDFLQTMARERLARVKDDERRISTLELRAQAEAMDPRADAFAPA
                     LRRPAGAPLRVIAEVKRQSPSAGMIRDAYDPVSIAASYEDAGASAISVLTEPDRFLGS
                     IDDLAHVRERVKVPVLLKDFVVHERQIYEARVRGADAALLIVAMLSPGQLRDYAAVMK
                     AIHLEPLVEILDDRDLDQALALDTQAIGVNNRDLRTLAMRRGWAEGLIREIPPNRVRV
                     GESGYSKRAEIEALERAGGDAALVGESLLKDGDPANALRRLLGREETPR"
     gene            21144..21782
                     /locus_tag="VFR25_02860"
     CDS             21144..21782
                     /locus_tag="VFR25_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9940019.1"
                     /translation="MRTRIKICGITRPEDGLAALTAGADYLGFILTESPRRLTLDQAR
                     TLRRALPPEAQVVGVFAEEPPEIVSTFARELALHAVQVGGWGDAAPDDAGTAEIWHVL
                     RAAELPDPVTLPMVPLRTYVLDAHDPKLPGGTGKRADWEWARRCIEVGRRLIVAGGLE
                     AANVGPLVRDIRPFGVDASSKLESAPGRKDPGKVRAFIDAVHAADHDRPKRS"
     gene            21784..22977
                     /gene="trpB"
                     /locus_tag="VFR25_02865"
     CDS             21784..22977
                     /gene="trpB"
                     /locus_tag="VFR25_02865"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9940020.1"
                     /translation="MTPQSPREGRFGAFGGIYVPETLLTPVLELAAAYDEARRDPAFL
                     AELARELHDFAGRPTPLTLASRFADACGLERVYLKREDLLHTGAHKINNAIGQALLVR
                     RMGKTRVVAETGAGQHGVASATACARYGLECVVYMGEVDMDRQRPNVERMRLLGAEVR
                     GVASGSRTLKDAINEAMRDWSASVRTTHYLLGSVLGPHPYPTMVRDFHSVIGREAREQ
                     ILAVEGRLPDRLVACVGGGSNSIGLFHAFLGDASIAMVGVEAGGEGITTGRHAARFAE
                     PKPGVLHGTRSLVLQDDSGQVRETHSISAGVDYPAVGPEHAALGAAGRVRYDSATDAE
                     ALSAFDVLGRTEGILPALESSHALAWVMREGRAGRIRREERILVNLSGRGDKDLGVVM
                     AARRG"
     gene            complement(22985..>24493)
                     /locus_tag="VFR25_02870"
     CDS             complement(22985..>24493)
                     /locus_tag="VFR25_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940021.1"
                     /translation="HLELSGRTPLALLATVLALLSKETAVALVPAIALIRPLSGRRPI
                     RSTIPVVAAYAGLVAIWGAVHPGIRGLLTHRFESSDPAVAYLSAARADLAGSMAKVGR
                     TLVNVPIQGAQVAWPVELDMVLPFAIGVLALGIWMAWRSGPTDSTANVSVGRILFLAA
                     LLTLPAIVVVAGLVKILSPYYAVLAGIGTSLAIGLGLARAPRILAAALTVAFLGMGFA
                     CRGMDLGPSVPTERTLKGPSDRLAKLEQSFRQVLPRIEGPTRLLVSVQAPDDRDLALH
                     LIRLQVARIWYRNPKLDTMYPERLTAGRTPEILAWVSQELSVYAIDPATLGVIPSGMP
                     DSSGYRTTLRAYARGLAGSGQTDRAVELLLRMKTGEEWEAAYNRRLAGALLLVAGRKA
                     EADRILEDTPRFNRNDAIAIVYEVASIPSSLDLDAAILDAVGLGASDAEAIRSVLRQL
                     ALSRYPAATIRFATRLLALRPGDSEARTVLQMLQRGEDANRVTIPVEHDIPW"
BASE COUNT         3989 a         8390 c         8560 g         3554 t
ORIGIN      
        1 gagcgagtgg tcaagcgcgc ggggatcgtc taccaccgcg cggaggggga agctgtcttc
       61 tacggaccca agatcgacat caagatctcc gatgccctgg ggcgcgcgtg gcagtgctcg
      121 accgtccagt tcgacttcaa cctgccgcgc cggttcgagg tgaactacgt cggctcggac
      181 gggcaggagc atcaggtcta catgatccac cgggcgctcc tcggctcgct ggagcgcttc
      241 ttcgcgacct tgatcgagca ctacggcggc gacttcccgg tctggatcgc gccggtccag
      301 gtccagctca tcaccatcgc ggacgcccag atcccgtacg ccgaggagat caaggcgatg
      361 ctcctcgacc ggggcgtccg ggtcgaggcc gacctccgcg aggagaagat gggggccaag
      421 atccgggacg cggagctcct caagatcccc tacatgctgg tcctgggccg ccaggaggtg
      481 gaaaaccggg cggtctccgt caggagccgg aagaagggga acgagggatc ggtgccgctg
      541 gatcgctttg tggaaaatct cgttgcggag gtcgaggcca gaacgtagac tggcctctcg
      601 tttcacgttc aagcaggagc cgacggcttc tcaccccgag gcgcaacgct accggggtca
      661 acgcgatacg gcccgcgcca cccgcgcggt cctgggccgg tgaaccatgg ggcagagccc
      721 tacccggtcc gtcgctccgt ccgtcgcctc ctgccactca aactgtagcc cggggcagag
      781 gagggtagcc gatcgccacc agcaaagaga tccgcgtcaa cgaccgcatc cgaattccgt
      841 cggtccgcgt catcgggccc gatggggagc aggtcggaat cctgagcatc cgcgaagcgc
      901 tcgcctacgc gcaggagcgc cagctcgatc tcgtcgaagt ctcgcccacc gccaagccgc
      961 cggtgtgccg ggtgatggac ttcggaaagt tcaagtacga gcagaacaag aagcagcaga
     1021 aggcgcggcg gaagcagcac gtcacgcacc tcaaggaagt caagctgcgg cccaagatcg
     1081 aagagcacga ctacaacttc aaggtcgagc atgggcgccg cttcctcgag ctgcacgaca
     1141 aggtgaagtt cacggtgatg ttccgtggac gcgagatggc gcaccccgaa gcggggacca
     1201 ggcttctcga gaaggtgatc aaggacctcg agctggtcgg ccaggtcgag atcccggcgc
     1261 gcatggaagg caggagcatg gtgctcctca tggtgccgcg tcccaccacg gcggccgcga
     1321 agcctgcgga ggccaagaaa ccggccccgg ccggcgcgcc cgcgcctcag cccgcagcac
     1381 cgcgggccga agcggcgaag accgcgaaac cctaggagtc gacgaatgcc gaagatgaag
     1441 accaaccgtg cggccgcgaa gcgattccgc cgcaccggaa ccggaaagtt gaagcgcgcg
     1501 cacgccttcg cgcggcacca cctcgggctc aagagccgga agcgcaagcg gaagctccac
     1561 gcgtccgcga tcaccgagtc gacggaacgg cgccacctac agaagtgtct accgtatggc
     1621 ggctgagccc tgacgggctc gtgccgaacc gaagaggaac ctagccatgc cacgcacaaa
     1681 gacggtcgtt ccgggccgca agcgtcgccg caaggtgatg accgcggtca aggggaaccg
     1741 aggcggccgc cgcaagctct accagacggc ccgcgaaacc ctgatgcgga gcctgcagtt
     1801 cgcctaccgc gatcgccgcg cgaagaagcg cgagttccgg aagctctgga tcgtccggat
     1861 caatgccgct gcccgccatc acggactctc gtacaacgcc ttcatccgcg gcctcaaggc
     1921 ggcctcggtg gagatcgatc gcaaggtcct cgcggatctc gcggtgcgtg acggcgaggc
     1981 gttcgcccgg ctggccgagg tcgccaagca agccaccatc gcggcctgat cggccgcaag
     2041 attccatgat cgagcgcctg gacgccctcc gcgccgaagg ggagcgcgcg ctcgccgacg
     2101 ccgcgtctcc cgcggacctc gaagcggccc gtgtgcgctt tctcggccgc aagagcgagc
     2161 tgaccgggat ccttcgcggg ctgaaagatc tcacacccga cgagcgcacg ctcgtcggcg
     2221 cccgcgccaa tcagacccgg gcccggctcg aggcgctgct cgcggaggca gaatcgcggc
     2281 tccaggccgc gtccgctccc gcgtccgcgc gcgacgtcac ccttccggga cgccgcccac
     2341 ccgtcgggca tcgccatctc atcccgtccg tcctgcgcga gctcgaaggg atcttccgcc
     2401 gcatgggctt cgcggtggcc gcgggccccg acgtcgagga cgactggcac agcttcggtg
     2461 cgctcaacat gccgaagggg caccccgcgc gcgccgccac cgacacgttc tacctcgagg
     2521 gggacagcat cgtcctccgg cctcacacgt cctccgtcca gatccgcgcg atgggggcct
     2581 ccaagccgcc tcttcggatc atcagccccg ggcgcgtcta ccggaacgag gctgtggatg
     2641 cgacgcacct cttcgagttc caccagatcg aagggctcta cgtggacgag aacgtcagca
     2701 tggccgacct caaggggacg ctcgatcgct tcctgaagga gttcttcggc cccaagacgg
     2761 agatgcgctt cagcccttcg tactatccgt tcgtcgaacc cggcgccgtg gtcgacatcc
     2821 gctgcttctt ctgcggcggg aagggatgca ctgtgtgcgg ccccttgtcg ggcggatggc
     2881 tcgagctgct tggcgcgggg atggtgcacc cgaacgtgtt ccgcgaggtc ggctacgacc
     2941 cggagcgctg gacaggattt gcgttcggcg tcggcgtgga gcgcctcatc atgctgcgcc
     3001 acggcgtgcc cgattcccga tacctcttcg ggaacgacat ccgcttcctg acccaattct
     3061 gaggagcgag tgaaagtcac ctggaactgg ctcggcgact gggtcgatct cgcaggcacg
     3121 ccggaagagc tggctcagct tctcgcctcg cgcgggcttc cggtcgaatc ggtggagcgc
     3181 ggcgtcacgt tcgatccggc catcgtggtc ggtcatgtcg tggagaaggg ccagcatccc
     3241 aatgcggatc gcctgagcct ctgctcggtc gacatcggcg gtccggcgcg gctctcgatc
     3301 gtctgcggcg cgccgaacgt ggcggcaggg cagaaggtcg ccgtcgcgca gatcggcagc
     3361 aagctcccgg acggcaccaa gcttcgcaag tcgaagatcc gcggggtgga gtcggaaggg
     3421 atgatctgct cggagcgcga gcttggattg agcgacgagt cgcagggcat ctgggtgctt
     3481 cccggcgatc cgcccatcgg cgctccgctc gcctcggtgg taggcggcgg cgatcccgtt
     3541 ctcgacgtcg agatcacgtc caaccgcccc gactgcatga gtgtgatcgg tctcgcgcgc
     3601 gagatcgcgg ctgcacgggg caagaagctc aagccgccgc ggccgctcgc ggcggccgga
     3661 tcgggcgcgc tcccgccggt cacgatcgag aacccggccg actgcccgcg ctacatggcg
     3721 cgcgtggtcc gcggggtcaa gatcggcccg tcgccggact ggctcaagaa gaggctcgaa
     3781 gcgaccggct tccgcagcat cagcaacgtc gtcgatgcga ccaactacgt tctccgcgag
     3841 cacggccagc cgatccacgc gttcgacgcc gccaagatcg gcggcttcgc gatcgtggtg
     3901 cggcgcgcga aaccgggtga gaagctcacg ctcctcgacg gtcgcgagat ggcgctcgac
     3961 gccaggcact tggtcatcgc cgaccgctcg atcccgatgg ccctcgccgg agtgatgggc
     4021 gggctcgact cgggcgtcac cgacacgacc gtcgacgtcg tgctcgagag cgccgtgttc
     4081 gacgccgcgc tcacgcaggc gaccgcgaag tcgctcggaa tcgcgaccga cgcctccgcg
     4141 cggttcatcg agggcgtcga tccggtcggg gtggagcgcg cgctcgacac gacggcgcgc
     4201 cttctcgcgg agatcgcggg gggcacggtc acgcgcgaga gcgtggacca gtggcccggc
     4261 cgcagggaac acgcgccgat ttcgctctcg cgccggaagg tggagcggct tctgggtatc
     4321 ccgctcgacc ccgcggccat cacgagcgcg ctctccacgc tcgagatcga gcccgccggg
     4381 agctgggcgg cgaaggaagg ggacgaggtc gccccgttcc gcgctccgtc gtaccggcac
     4441 gacctcgcga tccaggagga cctgatcgaa gaagtcggcc gggccgtcgg gtacgacgcc
     4501 attccgctcc ggctcgtcgc ggcgccatcg gggccagatc tcgtccgcac ggacgcgctc
     4561 gtcgcggagc gactgctcga tctcgcgacc gggctcggct tccaggaagc cctcacgaac
     4621 atcctcgtcg gcgacattcc cgcggatgct cgggaaggca tcggcgacga tcagatctgg
     4681 gagcttaaga accccatgag ccgcgagctc aagcacctcc gcgtcgggat cctgcccggc
     4741 ctggtcgagg cggccggccg aaatctcagg atgggcgccg cggacgtgcg gctcgcggaa
     4801 gcggggaagg tcttcctgtc ctcgccaccg ccgctcggca ccgagcgata cgaggcgggg
     4861 ctcatcctgg ccggaacgcc cgacgagtgg gaccatccgg acgcggacac cgaccgctat
     4921 ctcgagctca agggggcggt tgaagcgctc cttcagggct tcggcattga ctcgtgtcgg
     4981 acagcaacct accatgggcg aaactggagc gaaggcagtg gcgcgtcgat gcgcgtcggg
     5041 agcgaaaccg tggcccagat gggcgaggtc gccccgtcgc tcaaggcatc catgggtctg
     5101 gatcgcccgg cctgggccgc gatcctggat gtcgccgcga tcgagcgcgt cgcgcccgca
     5161 gtccggcgtt accgcgacat cccgcgctat ccggctacca agcgggacct tgcggtcatc
     5221 gtgcctcggc agacgcacca cgcggagctc gaggacgcga tccggaaggc ggggggagcc
     5281 cttctcgacc gggttcgtct cttcgacgtg ttcgagggac gcagcatcgg cgagggcaag
     5341 aagagcatgg cgtacgcgct cgagttccgg tccccggacc gcacgttgtc ggatcgggag
     5401 gcggatgggg cggtgcagtc gatcgtaggc gcgctcgagg cgcgtttcgg cgcggtgttg
     5461 cgcggcggac cctcggggcg caccggagac ctgacatgat ggccgagctg gatcaactgg
     5521 aagatcggat tcacgccgcg gtggggctcc tcgagaagta ccgggaggag aaacggcgac
     5581 tggaaagtga gaacggtgag ttgaaggatc gcgtccatgc actcgaagtg gccgcgggga
     5641 agcaggccga agacctgaag ccgaagatcc aggctctcga ggatgagcgc acggcgctcc
     5701 tcgacgagcg gcgggtcgtc gcgcggaggg tcgaggagat gatgtccaag ctggatcagc
     5761 tccacaaggc ggtccacgct tgaggtagac ggtccgacgg aaggccctcg ggggctcgga
     5821 ctgtcgtggg aacgatcgat ctgagggatc gcgatgacga ccgaaccgaa cacgcagagc
     5881 gtgacgatct taggcagcga gtacaagatc cgcggcgccg atccggatta catccaagtg
     5941 gtggccgcct acgtggacgg caagatgcgg gaattggaag gccgcattcc gcccggcacc
     6001 ccggccaagc tcgcgatcct gacctcgctc aacattgcgg acgagctctt ccgcgaacgg
     6061 gaagagcgca cgcggcgtga caacgaactg agggagcgga ccaaggaatg gggccgcctc
     6121 ctcatggaag taactcgcga gagctagtcg atccccgacg acagagaccc ggaaccccgg
     6181 aggcgtatcg aagagcgtat ctctacaacg gctggaaacg caccctgcca tgctcgtgat
     6241 gtgccaggca tttttgagcc acaactcgaa ggaagggagc cggaaggttc ggcggagtga
     6301 cggcccgctt cacgcgggcg acgcaaaccc ggatccaggg cgcccacctg gatatttcca
     6361 ggttcaaaag tctcgcaccc acggcattgg cggggcgctt tatttccagg ggaggccgtc
     6421 gtcatgacgc tcgcgccatg gttgatcatc ctgatcgcgc tcggagcagc gctcgtcgcc
     6481 tttttcggcg gctggatcgc gcaccggcgg gtcggtgaag gaaagctcct cagcgccgag
     6541 gagctggcca agaaagtcgt ccgcgaagcc gagcgggaat ccgagaacct caagaagggc
     6601 gcgctcctcg aagccaagga cgagtggttc cgggagaagg cgaagctcga ccgcgaggtc
     6661 caggggcaga aggccgaggt ccagaaggcg gagcgaactc tgcacgagct cgagtcgaac
     6721 ctgaaccgcc gcgccgaggt gctcgacaag aaggagcggg accagaagcg gacggaccgc
     6781 gagctcgaag tgaagggcga agccctcgcg gagcgggaca aggatctcgc gcgcatcctg
     6841 aacgagcaga acgtccggct ccagcggatc agcggcatga cgatggacga ggccaagtcc
     6901 cagctcatcg ccaacatgga gaacgaggcg cgagccgacg ccgcgcggaa gctccaagag
     6961 atccgcgagg acacgatccg gaacgcggag aaggaagccc gcgagatcat caccctcgcg
     7021 atccagcgca cggccgccga ccactccgcc gagaccaccg tatcggtggt gcatcttccc
     7081 aacgacgaga tgaaggggcg catcatcggc cgcgaggggc gaaacatccg cgccttcgag
     7141 accgcgaccg gcatcgacgt catcatcgac gacacgcccg aggccgtgat cctctccggc
     7201 ttcgatccgg tgcgccgcga gatcgcgaag cgctcgctca cccgcctcgt caccgacggg
     7261 cgcattcacc ccgcgcgcat cgaggagatc gtcgccaagg tccagaagga agtcgaggac
     7321 cagatcctcg agctgggcga gggggcggtg ctcgaggtgg gcgtgcactc gatgcatccc
     7381 gagatcgtga agctcctggg acggctccag taccggacct cgtacggaca gaacatcctc
     7441 cagcactcga aggaggtggc ctggatctgc gggatcatgg cggcccagct cggattcgac
     7501 gtgcagctgg cgaagcgcgc gggactcctc cacgacctcg gcaaggccgt gacgcacgag
     7561 gtggaggggg cgcacgcgca gatctccatg gacttcgcga agaagtacgg cgagccggag
     7621 gaggtcgtga aggcggtggg ctatcaccac cacgatccca cggcggagaa cctctatgcc
     7681 gtgctcgtca tggcggcgga cgccgtctcg ggcgcgcggc cgggggcgcg gcgcgagagc
     7741 atcgagaact acgtgaagcg gctcgaggcg ctcgagaaga tcgccgacag cttcccgggg
     7801 gtggagaagt cctacgccat ccaggccggc cgcgaggtgc ggatcatggt cgagccgaag
     7861 aacgtcgacg actcgcgagc cgcgacgctc gcgtccgacg tcgcgcggcg cgtcgagcgg
     7921 gagctccagt atcctgggat gatcaaggtc gtcgtgatcc gggagacccg cgcgatggag
     7981 tacgccaagt agccggcggg atcggaaccc aaccgtgaac atcctcttcg tcgcagacgt
     8041 gtacggctcc ccggggcgcc gcgccgtccg cgagctcctg cccgacatcg tctcctccat
     8101 gggcatcgac ttcaccgtgg tcaacgtgga gaacgccgcc gcgggattcg gcgtgaccaa
     8161 ggacacgctc gaggagatga agacgctcgg cgtggacgcc atgacgagcg ggaatcacat
     8221 ctgggacagg cgcgagtccc tcccgctcct ggattcggag ccgctcctcc tccgcccgca
     8281 caactatccg ccgggcgtgc ccggaacggg ttcgcgcgtg ttcacgagcc gggacggagt
     8341 gaaggtgggg gtgctcaaca tgatggggcg cgtgttcatg cgcgagctcc tctgtccgtt
     8401 ccgcaccgcc gaccgcgaga tcgagcgtct gaaggacgaa ggagcccgcg tcgttctcat
     8461 cgacttccac gccgaagcca ccgcggagaa ggtcgcgctc ggctggtacg tcgacggtcg
     8521 cgcctcggcc gtgctcggaa cccacacgca catccagacc gccgacgatc ggatcctccc
     8581 cggcggcacc ggctacatca ccgattgcgg catgaccggt cccttcgatt cggtgatcgg
     8641 ggtcaagaag gagcaggcga tccagaagtt cctcacgctc ctgcccacgc gcttcgaacc
     8701 cgcgggcggg gacgtgcgcc tgaacggcgt ccatctggac atcgacgaga ccacgggcct
     8761 ctgccgtcac atcgagcgca tccagctcgc cctcgagccg gcggtgcggc cggccacggc
     8821 gtgagcgcca cgatgaccgc cgtccgactc gccggcgggc cgctcgcgga cgagatcaag
     8881 gcgtcggtgc gcggacggtt cgagcggctt cgcgcgaagg ggatcgtgcc cggtctcgcg
     8941 gtggtgacgg taggggagcc tcccggcggg aatccctatg tgcgctcgaa gtgccgtgcc
     9001 gccgaggagc tcggagcgca ggcgcacgtg acgcggcttc cgcacaccgc gtcggaagcc
     9061 gaggtccgcg ccacgcttca agatctgagc gatcatcccg cgtaccacgg catcatcctc
     9121 cagctcccgc tcccgtcctc gctgcacgag gagcggcatc tcgaggacat cgtgcccgtg
     9181 aaggacgtcg acggcatcca tccgatgaac gtgggccgct gggtcagcgg acttccggcg
     9241 cacaggcccg cgacgcccgc cgggatcgtg gagctcctgc gccgccacgt cggcgatctc
     9301 tccggcaagc ggatatgcat cgtgggaagg agccgcatcg tcggccggcc gctctccgtg
     9361 ttcctctcgg agcgcgcgac cggcatgaac tccacggtca cgctgtgcca ttcggcgtcc
     9421 cgcgatctcg cctcgatcac gcgcgaggcg gagatcctcg tcgtcgccat gggacacccg
     9481 cgcgccatcg gcgccgcgca cgtgcgcccc ggcgccatcg tggtcgacgt gggaatccac
     9541 gcgatcgaga accccgcgcc cggcggtccg aaatacgaag gcgacgtcga ttacgaggcc
     9601 gtgagcaaga tcgcctccgc cgtcaccccc gttccgggcg gcgtgggtcc cctcacggtg
     9661 gcggtgctcc tccgcaatct ggccgatgcc gcggaagcgg cggcggagcg cgcgtgagcc
     9721 ctcgcatcgg cgccgcggag atcgatttcc accaagggca gccgcgcgtc tacacggttc
     9781 acgagattgc gcaggcgatc aatctaacgc tcggggatca cggcgtcgtc tccgtgcagg
     9841 gcgagatctc cagcttcaga cgccagtcca gcgggcacat ctacttcgag ctcaaggacg
     9901 acaacgcccg ccttcgcgtt tcctggttcc ggaaccgcat tcgccagtcc atcgacgcac
     9961 tcggggacgg cgtccaggtc caggtcgacg gcaggctcga gttctacgcg acgggcggca
    10021 cggtgtccct catcgcggat cgcgtatcgc ccgtggggct cggcgcgctc caggcgcgct
    10081 tcgacgcgct caagcgcgcg ctcgaggccg agggactctt ccgccaggag cggaagcgac
    10141 cgctgcctgc atatcccacc cggatcggca tcgtgacatc gccttccggc gccgccgttc
    10201 gcgacatgct ccggatcctc cgtcagcgcg caccgtacgt ccgcgtggtc gtggcgccga
    10261 gctcggtcca gggggcggga gccgcgttcg agatcgcggc cgccatcgca ctcctgaacg
    10321 actgggggaa ggtggacgtg atcatcgccg gacggggcgg cgggtcgatc gaggatctct
    10381 gggcgttcaa cgaggaagcc gtcgtccggg ccatcgcggg atcgcgcatc ccggtgatca
    10441 gcgccgtggg gcacgagacc gacgtcacgc tcgcggatct cgtcgccgac ctgcgcgcca
    10501 tgacgccgac gcacgcggcc caggtcgtcg ctccgagccg cgaggacgtc tgtgcgaccc
    10561 tcgacaaggt cgcgaagcac gcgcgggacc gcctcgggcg cgagctgcgc gaggcgaggg
    10621 cgcatctcgc gggcatccgc aatcaccacg cgctccgcga gcccggtcac atgatccgga
    10681 acaatcgccg gcgtgtcgac gagctctcgg actgtcttgg ccgcggtctc ggcgactggg
    10741 tcctgaagcg ccggcgccgc gtggatcagc tcacgggcgt gctccacgcg catacaccga
    10801 cccgaacgct cgagcgctca cgcgaacgcc tcaccgcgct cgcggatcgc gcgcggcgct
    10861 cgacgctctc tcaggtccag cgcgaccgca ccgccgtatc gggcgccggc cggctcctcg
    10921 cttcctacga tcaccgcggc gtgctccggc gcggctacgc gctcgtctgg agtcccgacc
    10981 ggaagctcat cgcgcgcgga gggacgctcc ggcccggaga cgacgtccaa gtcgagttcg
    11041 cggacgccaa ggccgaggcc aaggtcgcga gggtggaccg aaagcccgag tcctcagaag
    11101 gaggtgctac atgacgccca aagccaacgc accggcgccg gacgatgccg gcgcgatcac
    11161 gttcgagcag atgatggggc ggctccagga cctcgtcgac aagctcgagg aaggacagct
    11221 gagcctcgac gaatcgatcc gctcgttcga ggaagggatg gcgctggtga agaagtgcac
    11281 cgaggtgctc gatcgggccg aggagcggat ccagaagctc acgcgggatt cgtcgggacg
    11341 accgatgacg agcccgctcg acggggacga ggacgaggcg ggaacgggtg gcgacgagct
    11401 cccgttttga gcgatacctc gagcgcgccc ggcccaagat cgaggccgcg ctcgaccggc
    11461 tccttccatc cgcgtcccgc gagccgaaga cgctccaccg cgcgatgcgc tacagcgtgt
    11521 tcgcgggagg gaaacgcctt cgccccgcgc tctgcctgct cgcttgcgag tccatctcgg
    11581 gcaagagcac gccggctctt cccgcggccg cggcgctcga gatgatccac accttcagct
    11641 tgatccacga cgatctgccc ggcatggatg acgacgatct ccgccgcggc cgtccgacga
    11701 accacaaggt cttcggcgag ggcatggcga tcctggcggg ggacgcgctc ctctcgctcg
    11761 cgttcgagac gctctcccgg tccaggaatt tgaagcgcgg cgccaatcct tgcgcgctcc
    11821 tcgagacggt ctgcatggcg acgggaccgg agggaatgat cggaggccag gccatggacc
    11881 tcctcttcga gggacgcaag gtgccgctcc ggagcgtcta cgccatgcac cggcggaaga
    11941 ccggcgctct tctccacggc tcgctccggc tcggagccca gatcggcggc gcgagcgccg
    12001 cggagctgcg tgcgttccag gcgttcgggg agcgcgtcgg gctggcgttc cagatcgggg
    12061 acgatctcct caacgttcgg tccacgcgaa aggagctcgg caaggcgacc ggatcggatc
    12121 gcgcgcgcgg caaggcgacg gttccagggc gcgttggcgc ggagcgcgcc gagcgcgagg
    12181 ccgcccggct ccttcgcgaa gccgcggcca tcgcgcccaa gctcgggcgt cggccggagg
    12241 agttcgtctc gatgaccgag ttcctgctgc acaggactca ctagcatgcc gatctcgtcg
    12301 agtccgctcg tccacgccct cgcttgcggg ctcctggtgc agctgagcaa ggtgttgacc
    12361 ttcctcctgc gggagaagaa aatcaacgtg cggcgcctcg tggagacggg cggcatgccg
    12421 agctcccacg cggcatccgt atgcgcgctc agcacttgcg tgggactccg cgaagggatt
    12481 gggtcggtcc tgtttcaggt cgtcctcttc ttcagcctga tcgtcatgta cgatgcggcc
    12541 gggctgcggc gcgccgcggg gcgtcaggcc accctcttga atcgaattct gcacgagcac
    12601 atacagcttc cgggaccgcc ccacgagcgt cttcgtgagc ttctgggaca cacgcccatc
    12661 gaggtcctgg tcggggccgt gatcggcatc ctgttctcgc tcgccatcta tcgctctccg
    12721 ggaggcatgt gagcaccgac tacctgtccc aagtacacgg tccggacgac ctgaagcggc
    12781 tcccgaccga cgcgctcccc gcgctcgcgg ccgagatgcg ggagcgcatc atccaggtcg
    12841 tgtcgcgcac cggcgggcat ctggcgccaa gcctcggcac cgtggagctg acgatcgcgc
    12901 tccattacgt ctacgacacg ccgcgcgacg tgttgatctg ggacgtgggg catcaagcgt
    12961 acggacacaa gatcctgacc gggcggaacg agcgcttcga cacgcttcgc caggaggcgg
    13021 gcctctccgg cttcccgcgc cgcgtcgaga gcgagttcga tcctttcggc accgcgcacg
    13081 cctcgacgtc gatctcggcc gcgctcgggt tcgcctgcgc gcgtgacttg cggggcgaca
    13141 agaaccgcgt gatcgccgtg gtcggcgacg gtgcgctcac gggcggcctc gcctacgagg
    13201 ggctgaacca ggccggcgtg ctggggagcg acatcctcgt cgtgctgaac gacaactcga
    13261 tgtccatctc gcccaacgtc ggcgcgatcg cgcgctacct gactcggctc tcctcgtcgc
    13321 ccacgtaccg caggttcgag agcgatgtct gggatctcct cggcaagctc ccggcgggac
    13381 ggaaggcacg gcggctcgtc ggccgggtca aggaaggcat gaagaacctc atggtcccga
    13441 acatcctgtt cgaggagctg gggttcaagt actacggccc ggtggacggc cacgatctgg
    13501 gcgagctggt ggagctcctg cgtcagctca aggacgtgaa gggcccggtg ctcctccacg
    13561 cgctcacgcg gaaggggaag ggctacaagt tctccgagga cgactcgcgc aagttccacg
    13621 gtgtcgcctc gttcgacaag ctgaccggag cgagcgcgaa gaaggccggt gggacctccc
    13681 ccacgtacat ggaaatctat ggccagacca tgatcgacct cgcgagcgcc gaccgctcca
    13741 tcgtggcgat cacggcggcg atgaccgacg gcacggggct cgccaagtac gcaacgacct
    13801 tgcccgcgca gttccacgac gtgggcatcg ccgagcagca cggcacctgc ttcgcggccg
    13861 ggctcgcggc ggcgggcatg aagccgttcg tcaccatcta ctcgacgttc ctccagcgcg
    13921 cgtacgacca gatcgtgcac gacgtggtcg tccagaacct tccggtccgc ttcgcgctcg
    13981 atcgggcagg tttcgtgggc gaggacgggg cgacgcacca cgggctcttc gacatcgcgt
    14041 atctgcgctg ccttccgaac gtggtcctga tggcgccgaa ggacgagaac gagctcaggc
    14101 acatgctcgc gaccatggcg agctactccg ccggtcccat cgccgtgcgc tacccgcgcg
    14161 gcagcggtcc gggcgccgcg ctcgatccca agctcacacc gcttcccatc ggtgaagggg
    14221 agctcctccg gaacggaggc gatctcctcc tcgtcgcgta cgggacgatg gttcccgccg
    14281 cggagaaggc cgcacagctc ctcgaggccc gcgggatcaa ggccgcgatc atcaacgctc
    14341 gcttcgccaa gccgctcgac gagcggttga tcctcgagtg ggcgcgccgc gtgccgcgtg
    14401 tcgccacgct cgaggaaggc gcgctccccg gaggcttcgg cgacgcggtt ctcgaattcc
    14461 tcgccacgca gagcatcgat ggcctgcgcc ttcgccggtt tggagtgccc gaccggctct
    14521 tcgatcacgc gacccgcgac gcgctcctcc gcgccgccgg actcgacccc gagacgctcg
    14581 ccgcggagct ggaacggttc gttcgcggcg aatcccgatc cacaccggaa cccatcgctc
    14641 ccgccgtcac gcgcgcatga cccgtccgcc ccgccacgtg gggatctttg ggaatcggga
    14701 caaggcggag gtgcgcgcgc tcgtgcccga gcttgcgcgg tggatccaga aacaggggat
    14761 caaggtctcc gcggagtccg gaatcacgcg ggggcgcggc ctctcgctcg cgtcgctcgt
    14821 gaagaaggtc gacgtcctcc tcgtgctcgg cggcgacggc acgatgctcc atgccgcccg
    14881 cgcggcctcc caggcaggcg tcccgatctt cgggatcaac ctgggcgggc tgggcttcct
    14941 caccgagacg agcgacgacg agctctaccc ggccctcgag cgactgttcc ggggagacgt
    15001 ccacacggaa cgccgcctca tggtggaagc gcgcgtgaag tcgcggagcg gccgcacgtg
    15061 gtccgcggtg gggttgaacg acgccgtgat ccacgctcgg gatcggtcgc gggtcgtggc
    15121 catcgacgtg cgtgtgggtc cgacgccgat cggaacgctg gtcgcggatg ggctcatcgt
    15181 cgcgactccg accggctcga ccgcctactc gctctcggcg ggaggaccca tcgtcgggcc
    15241 caccatcgag gcgctcatcg ccacgccgat cagcccgcac acgttcgcgt tccgcccgtt
    15301 gctggtcccc gtgggggaga cggtggtcgc acgcgtgcgc gcgggacatg ctcccgccgc
    15361 ggtcacggtc gacgggcagc agtcgcggcc gctctcgttc gacgacgagg tgaccttccg
    15421 ccgcgccaag gcgcacgtcg acctcctcgt gctcgagcgc gggacgttct acgaagtgct
    15481 ccggaccaag ctcgcctggg ccgagctccc gcgcacgcgc gcggcgcgga ggtgacccgg
    15541 tgctctcctg gctttccatc caggggctcg ccctcgtcga ctccgtcgag ctttcgctcg
    15601 atcccggcct caacatcctg accggcgaga ccggggcggg gaagtcgctc gtcctgggat
    15661 cgatcgggct cctgctcggg gagcgcgccg acgcatcctg gctgcgcgcc ggcgagacgc
    15721 gcggatccgt cgaagccgtg ttcgatctgg gcggcaggcc ggatctcctg gcttccctgg
    15781 ccgagcttgg atgggagacc gaggagggcc ggctcctcct gcgccgtgag atccaggccg
    15841 aaggcaaagg acgcgcgttc gtgaacggtc gcgccgcgct cttagcacag ctcaaagcga
    15901 tcggagaacg gctcgtcgac ctgcacggac agcacgagca ccagcagctc ctcgatcccg
    15961 cgcgccagac cgacttcttc gacggctggg cgggactcct gggtgagcgc cgcgcgctgg
    16021 aagaggagcg cgcgtcgatc gtcgaggagc gcgccgccct gcgcgccgcg cgcgagcggt
    16081 gggagcgcga gcgcgaggcc gaggggaccg atcgcgagga cttcgaggag ctcgagcggg
    16141 cgtcgctcga tccagacgag gaacagaagc tgaaagaaga ccgggagcgg ctcgtgcatc
    16201 gcgagcggct cctccgcgcg ctcggcgagg cgcgccgcgc ggccgcggac gacgaggacg
    16261 gcgcggtcgc ggcggtcggg cgcgccgtcc gcgcccttcg cggggcagct gcgctcgacg
    16321 ccacggtcca ggacgtggcc tccgaggcgg agcggctcca gggcgagctc gcggagctcg
    16381 ggggccgcct ggagcgcgag gaggagcgcc tggtcgacgg cgttctcgat cccgaggcgc
    16441 tcgaggcgag gctcgatctc atccatcgct tgaagcggcg ccaccggacc gacctgccgg
    16501 gcctcgtggc gctccgggag gagctccgcg ccaaggtgcg cgcgttcgat ccctccggcc
    16561 gcgagctcga caaggcggag cgctcgcacg acgagcggct gcaggccttc gagcggaggc
    16621 tcgacgcctt cctcgcgcat cggaacgacc gctacgccgc gttcgagtcc gatgtcgggg
    16681 cccggctcgg gacgctcgga ttcaaccgct ccgcgctccg ggtgcgcccc gcggacacgg
    16741 ggcgtgcggt cgttgacccg gcgccgatcc cgcacctaga attcgcgttc cagccgaacc
    16801 ccggcgaagg ggagcgcccc cttcgcaaga tcgcgtcggg cggggagctc agccgcgtca
    16861 tgctcgccgt gaagtcgctc atggcggagc gcgatcaggt cgcggttctc gtcttcgacg
    16921 aggtggatca ggggatcggg ggcgcggtcg gcgaagaggt ggggcgtctg ctcgaagcca
    16981 tcgggcagcg ccggcaggtg ctctgcatca cgcaccttcc gctcatcgcc gcgcacggcg
    17041 cgcgccattt cgaggtggcc aaatcggtcc aggccaagcg cactcgcgtg tcggtgcggg
    17101 cgctcgagcc caaggagcgc gtggaggagc tggcccgcct cctggccggg gatcgcgcca
    17161 ccgagaccac acggacgcaa gcacgggagc tcctcgtggc cgccgcgagc ggcggccggg
    17221 agaccaagaa ggagccgcgg cgcgccgcga cacgacgagc atgatccgcc ccagcctcga
    17281 cgactaccgg cgcctcgcgc gcgagcacaa cctgattccg gtcacgcgcg agatcctcgc
    17341 cgacttcgac acgcctgtct ccgcgttcgt gaagctgaac cgcggcgacg cgtcgttcct
    17401 gctcgagagc ctcgaggggg gcgagacctg ggggcgctat tccatcctcg gcttccgccc
    17461 ctcggtcgag ttccgatcca gcggcaccaa ggtcgagatc cggcgcggcg agcgcaccga
    17521 gcggatggag cgcgaggatc cgctcgccgc gcttcaagac ttgatcgcgg acttccgcgc
    17581 cgcccccgtt ccggatctgc ctccattcag cggaggcgcg gttgggatgg tcggctacga
    17641 ctacgtgcgc ttcctcgagc gcctgccgtc ccggctgacc gcggaccgcg actatcccga
    17701 cctccagttc gtcttcccgg acgtcgtgct cgtcttcgac aacttccgcc accggcttca
    17761 ggtagtcgtc aacagccgtc ccggctcggc gcccgaccgc gcgtacgccg aagccggcgt
    17821 cgtgatcgag gagatgatcg cccggctcgc ggccccgatg ccgaacccgg ccccggtgcc
    17881 tcacccgaac ggcgacgtgc gcttccggag cacgatgggg gaggatgcct acaagaaggc
    17941 ggtcgtgaag gccaaggagc acatccgcgc gggggacgtg gtccaggtcg tcctggcgca
    18001 ccacctcgag gcggacgtcc cggtggcgcc gctcgacgtc taccgcgccc tgcgcgtgct
    18061 caatccgtcg ccctacatgt tctacctgcg cttcgccgac cgctccgtcg cgggatcctc
    18121 gccggagatc ctggtccggg tccacgggga ccgcgtcacg ctgcgtccga tcgcgggcac
    18181 gcgccgccgc ggagccaccc gggccgagga cacggcgctc gagcacgagc tcaaggaaag
    18241 cgagaaggat cgggcggaac atgtcatgct ggttgacctt ggacggaacg atgtcgggcg
    18301 gatcgcggcg atcgggtcgg tccggacgac cgagctcctg acgctcgagc gctactcgca
    18361 cgtgatgcac ctcgtgagcc acgtggaagg acgccttcga aacggcctcg gcgcgttcga
    18421 cgtgctccgg gcctgcttcc cggcgggcac ggtcagcggg gcgccgaaga agcgcgcgat
    18481 ggagatcatc gagtcgctcg agaccacccg ccgcggcgcg tacgccggcg cgatggggta
    18541 catcgacttc caggggaacg ccgacttctg catcaccatc cgcaccgcca tcatcgacgg
    18601 cggccgggtc cgatgcggcg tcggcgccgg catcgtggcg gattccgatc ccgacgagga
    18661 gtggaccgag acctgcaaca aggcgaaggc ggtcgaggag gcggtccgcc tcgccgcacg
    18721 gggactcgaa acatgatcct gatgatcgac aactacgatt ccttcacctg gaatctcgtc
    18781 cagtacctgg gcgagctcgg cgacacaccc atcgtgaagc ggaacgatgc catcacgccg
    18841 gacgagatcg cggccgcgaa gccgcgcgcg atcgtgatct ccccgggccc ggggcgtccg
    18901 gacgatgcgg gggtgagcaa ggacgtgatc cggcgcttct ccggaacgat tcccatcctc
    18961 ggtgtctgtc tcggccacca gtgcatcggc gaggtgttcg gcgggcgcgt ggtgcaggcc
    19021 gagcggctcg tgcacgggaa gacgtccgcc atcatccaca cggggcgcgg gctcttcctc
    19081 gacgtggaca atcccttcca ggcgacccgc taccattcgc tcatcgtcgc gcgcgagggg
    19141 cttccggaca cgctccaggt catggcctgg acaccggaga acgagatcat ggccctccgc
    19201 catcgcgagc acgagacctg gggcgtccag ttccaccccg agtcgatcct gacgacgtcg
    19261 gggaaggaca tcctccggaa cttccttctc agcctgcctg cgccagccac cgcggcgcga
    19321 tgatccggca gctcctcccg aggctcgtcc gcggcgaacg gctcgcgcgc gaggagaccg
    19381 ccgccgcgat ccggtcgatc atgcggggcg gcgaggaccc cgagctcgtc ggagccttcc
    19441 tcatggcgct cgcgcagcgc ggcgagaccg ccgaggagct ggccggcggc gcggaggcgt
    19501 tgcgcgcgga ggcggttccg tttcccggcg agggcccgtt tctcgacacg tgcggtacgg
    19561 gcggggacgg cacccacacc tacaacatct cgaccgtggt cggattgatc gcggcggccg
    19621 cgggacagcg cgtggccaag cacgggaatc ggtcggtctc gagccgctgt ggcagcgcgg
    19681 acgtcctcga agcgctgggc gcccgcgtcg atctcggccc cgagagcgcg aagcgcgcgc
    19741 tcgaggagac aggcttctgc ttcctctacg ccccgcgctt ccacccctcc atgaaacagg
    19801 tggcgggcat ccggcgttcg ctcggcgttc gcacgctctt caactgggtc gggccgctcg
    19861 cgaatcccgc ccgcgccacg caccagctcc tcggcgtctc cgatcgcgca cgcgtcgacg
    19921 tggtcgccca ggtgctcgcg gcgctcggag ccgagcgcgc tctggtcgtg cacggagcgg
    19981 gcgggctcga cgagctgtcg ctcgaacatg ggaatcacgc ggtctgggtg gagcgcggcc
    20041 gggagccgcg cggcgtcacc gtggaggcgg atgcgatcgg acttcctccg gcgtccgtcg
    20101 cggagctcga aggcggcacc cccgaggaga acgcaggcca cgtgcgggcc gtgctctccg
    20161 gacgggccgg agcccggcgc gacgcggcga tcctgaacgc agcggccgcc ctctgggtgg
    20221 cgggcgccgc ggactcgctg gaggaggcgg cggtcctcgc cacggaagcg ctcgactccg
    20281 gtgcagcggc ccgcgtgctc gatcgctacg ccgccttctc ccaagagatc ggagcggact
    20341 gaagtggact tcctccagac gatggcgcgc gagcgcctgg cccgcgtcaa ggacgacgag
    20401 cgccggatct cgacgctcga gctccgggcg caagccgagg cgatggaccc ccgcgcggat
    20461 gcgttcgcgc cggcgcttcg gaggccggcg ggcgccccgc ttcgcgtcat cgcggaagtg
    20521 aagcggcagt cgccgagcgc cgggatgatt cgcgacgcat acgatccggt atcgatcgcc
    20581 gcgtcctatg aagacgcggg cgcctcggcg atctccgtgc tcaccgagcc ggaccggttt
    20641 ctcggatcga tcgacgatct ggcccacgtg cgggaacggg tgaaggtgcc cgttctcctc
    20701 aaggacttcg tcgtgcacga gcggcagatc tacgaggcgc gcgtccgggg agccgacgcg
    20761 gccctcctca tcgtggcgat gttgagcccg gggcagcttc gcgactatgc tgcggtcatg
    20821 aaggccatcc atctcgagcc gctggtcgag atcctggacg accgggacct ggatcaggca
    20881 ctcgcgctcg acacccaggc gatcggcgtg aacaatcgag acctccggac gctcgcgatg
    20941 cggcgcgggt gggcggaagg attgatccgc gagatcccgc cgaatcgggt gcgcgtcgga
    21001 gagagcggct attcgaagcg cgcggagatc gaggcgctcg agcgggccgg gggggacgcg
    21061 gcgctcgtgg gagagagtct cctcaaggac ggcgatcccg cgaacgcgct tcgccggctt
    21121 ctgggacgcg aggagacccc tcgatgagaa cgcgcatcaa gatctgcggc atcacccgcc
    21181 ccgaagatgg tctcgccgcg ctcacggccg gcgccgatta cctcgggttc atcctcaccg
    21241 agagcccgcg ccgactcacg ctcgatcagg cgcgtacgct ccggcgcgca ctcccgcccg
    21301 aggcgcaggt ggtcggcgtc ttcgcggagg agccgcccga gatcgtctcc accttcgcgc
    21361 gcgagctggc gctgcacgcg gtgcaggtcg gcggctgggg cgacgccgcg ccggatgacg
    21421 cggggaccgc ggagatctgg cacgtgctcc gcgcggccga gctgccggat cccgtgacgc
    21481 tcccgatggt gccgctccgg acctacgtgc tcgacgcgca cgacccgaag ctcccgggcg
    21541 gaacggggaa gcgcgccgac tgggagtggg cgcgccgctg catcgaagtc ggacgccgtc
    21601 tcatcgtcgc cggcgggctc gaggccgcca atgtcggccc gctcgtccgg gacatccgtc
    21661 cgttcggcgt ggatgcatcg agcaagctcg agtccgctcc gggacgaaag gacccaggca
    21721 aggtgcgcgc cttcatcgac gcggtccacg cggccgatca cgatcgcccc aagcggtcct
    21781 gacgtgaccc cccagagccc gcgcgaggga cgcttcggcg cgttcggcgg gatctatgtc
    21841 cccgagactc tgctcactcc tgtcctggag ctcgcggccg catacgacga ggcccggcgc
    21901 gatcccgcgt tcctggcgga gctcgcgcgg gagctgcacg acttcgccgg tcgccccacg
    21961 ccgctcacgc tcgcatcccg gttcgcggac gcgtgcgggc tcgagcgcgt ctacctgaag
    22021 cgcgaggacc tcctgcacac cggcgcgcac aagatcaaca acgcgatcgg ccaggcgctc
    22081 ctcgtgcggc gcatgggcaa gacgcgcgtg gtcgcggaga ccggcgccgg acagcacggc
    22141 gtggcgagcg ccacggcgtg cgcgcgctac ggtctcgagt gcgtcgtcta catgggcgag
    22201 gtcgacatgg accgccagcg gccgaacgtg gagcggatgc ggcttctcgg ggcggaggtg
    22261 cgcggcgtcg cgtccgggag ccggacgctc aaggacgcga tcaacgaagc catgcgggac
    22321 tggtccgcat cggtgcggac gacccattac ctcctcggct cggtcctcgg gccgcatccc
    22381 tatccgacga tggtccgcga tttccactcg gtgatcgggc gggaggcgcg ggagcagatc
    22441 ctggcggtgg aaggacgcct gccggatcgt ctcgtcgcgt gcgtcggagg agggagcaac
    22501 tcgatcggac tcttccacgc cttcctcggc gatgcctcca tcgcgatggt cggcgtcgag
    22561 gcgggaggcg aggggatcac gaccggccgt cacgcggccc gcttcgccga acccaagccg
    22621 ggcgtcctcc atggcacacg gtcgctcgtg ctccaggacg actcggggca ggtgcgcgag
    22681 acgcattcca tctcggcggg cgtcgattac ccggcggtcg gcccggagca tgccgccctt
    22741 ggtgccgcgg ggcgagtccg gtacgacagc gcgaccgatg ccgaagcgct gagcgcgttc
    22801 gacgtcctcg ggcgcacgga gggcatcctt ccggcgctcg agtcctcgca cgcactcgcc
    22861 tgggtgatgc gcgagggccg cgcggggcgg atccggcgcg aggagcgcat cctcgtcaat
    22921 ctctcggggc gcggcgacaa ggatctcggg gtcgtgatgg cggcgcggcg aggctgagcg
    22981 ggtcctacca gggaatgtcg tgctcgacgg ggatcgtgac gcggttcgcg tcttcgcccc
    23041 gctgcagcat ctggagcacc gtgcgggctt cggagtcgcc cggacggagc gcgaggagcc
    23101 gcgtcgcgaa ccggatcgtc gcggccggat agcggctcag cgcgagctgg cgcagcacgc
    23161 tccggatggc ctcggcgtcg ctggccccga ggccgaccgc gtccagaatc gcggcatcca
    23221 ggtcgaggct cgacgggatc gaagcgacct cgtagacgat cgcgatcgcg tcgttccggt
    23281 tgaaccgcgg cgtgtcctcg aggatgcggt cggcctccgc tttccgaccg gccacgagga
    23341 gaagggcgcc ggcgagcctt cggttgtacg cagcctccca ctcttcgccg gtcttcatgc
    23401 gaagcaggag ctccacggcg cggtccgttt gccctgagcc ggccagaccc cgcgcgtagg
    23461 cacggagcgt ggtccggtac cccgacgagt ccggcattcc actcgggatc acgccgagag
    23521 tcgccggatc gatcgcgtac accgatagtt cctgcgacac ccacgcgaga atctcggggg
    23581 tgcgacccgc ggtgagccgt tccgggtaca tcgtgtcgag cttcggattg cggtaccaga
    23641 tgcgagctac ctgtaatcgg atcagatgga gcgcgaggtc gcgatcgtcg ggcgcctgga
    23701 cggagacgag gagccgtgtc ggcccctcga tccgggggag aacctgacgg aagctctgct
    23761 cgagcttcgc caaccggtcc gacgggccct tgagggtgcg ctccgtcgga acgctcggac
    23821 cgagatccat gccgcggcaa gcgaacccca tcccgaggaa cgcgaccgtg agtgcggccg
    23881 cgaggatccg cggcgcgcgc gccaggccga gaccgatcgc gagcgacgtg ccgattcccg
    23941 cgagaacggc gtagtagggg gacaggatct tcacgagccc ggccacgacc acgatggcgg
    24001 ggagggtcag gagcgcggcc aggaatagga tgcggccgac ggatacgttc gcagtcgagt
    24061 cggtcgggcc cgacctccat gccatccaga ttccgagcgc gagcacgccg atcgcgaacg
    24121 gaaggaccat gtccagctcg accggccagg caacctgcgc gccctggatc ggcacgttca
    24181 cgagcgtccg cccgactttc gccatggagc ccgcgaggtc cgcgcgagcc gcgctcaggt
    24241 acgctacggc gggatcgctg ctctcgaagc ggtgcgtcag gaggcctcgg atcccggggt
    24301 gcacggcgcc ccagatggca acgaggcctg cataggccgc cacgaccggg atcgtggagc
    24361 ggatgggcct gcggccgctg agaggtctga tcagcgcgat tgccggcacg agcgcgaccg
    24421 cagtctcctt ggagaggagc gcgagcaccg tcgccaggag cgcgagcggt gtccgcccgg
    24481 acagctcgag gtg
//
LOCUS       DASTKC010000064         9656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_756413, whole genome shotgun sequence.
ACCESSION   DASTKC010000064 DASTKC010000000
VERSION     DASTKC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756413"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFR25_02875"
     CDS             complement(<1..201)
                     /locus_tag="VFR25_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940022.1"
                     /translation="MSAAAVRPLAVTLAAALAFTSPARAAFEVRDGSPAALGAASLDL
                     ESVPFFEPAGFAASASHTTLYQS"
     gene            complement(198..1889)
                     /locus_tag="VFR25_02880"
     CDS             complement(198..1889)
                     /locus_tag="VFR25_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9940023.1"
                     /translation="MLKLALVGACALAVASTARADLVLNEVLYDPDGADEGHEFVELW
                     NPDSVAVPLDGVALETSDGARPGTWAVVWRANAGDIAGPRSPFVVGAARLLAALQNGP
                     DAIRLTRAGAVLDLLGYGELSDPGQYEGSPAPDAPSGASVARVEDGVDTELNRDDWAV
                     AAEPTPGAANHPDMRLRFGRAKLVLSPSVCWPGEPFVARASVRSSGRLPLFGARWRIA
                     CDLEASGSWVPGAAAPGVDLAPGESASVSLSLVDPAPGARRVRLRLEGALGEDAALSD
                     TVAAWLRSIASPVVLSEIAFHNTGSGEWIEIWAREPIEDVGSLTLADAASSPRPIDRG
                     SAPRSLAAGSYLVVAQDPAAVRSRCGLPDSIVVGVTGGWPSLNDGAEPGSPADMVALA
                     LADGVPVDAMTYAGGATVRDGSLERLSFDLPSGDPGSWSECVDANGATPGRANSLHAP
                     AGPSAGGHALLVTATRVLSVHARGSVPIVFRATEAARGRRLTVRVHDLLGRPVRTLTE
                     GQRLATDAAFVWDGNDDAGGPAPAGLYVVRAEALPEDGVAPLRSSLSIAVAGGRP"
     gene            complement(1959..4124)
                     /locus_tag="VFR25_02885"
     CDS             complement(1959..4124)
                     /locus_tag="VFR25_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9940024.1"
                     /translation="MKIELQATPELVALALIGAAIAIGLWAYLTRYPAVPAKRRGILL
                     ATRLLALLALLVASIAPVLRYPETSRARNRLLILVDHSGSMDLRDLSGGRSRRAAADS
                     AAQAVARDLGGRYEVRTASFDAALGPYDKDASKALAFQGGGETALGDAIRFSFNRVDP
                     DSVAAMLVLSDGVVNRGEDPEQALGAAVPLFGLTVGRHTDPPTVGIAGVETPPEIILG
                     RPTPLVVSVRQGSRQESRGTVRVSEEGRVLGRTEFALHGPGASARVSIPVTLLERGKR
                     FLTVDLVDVPDDPMRENKQRLVAVLARPAKRTIPVLASSWDWDLRSLARGIEEDTTRA
                     VARLTPSGSSQAVALGGAPRPFSSWLEDAEAVVVRYDSGTMTPERADALMRFLARGGG
                     VLLWIDPTPRPPVESALTRALSIQWRLFGQPLGPIASTELAPAGRTQDVALLSGDAAS
                     AASIWKDLPPVEPILSLGTAGSPLQTIVIGRVGNEAIPIVLAGSVGKGRVVLLNAAGV
                     YRWGLTAAGLTGKPGIEGSFFGGAERWLAGGWEDRPVSITAPDITPVGRPIAVRVATT
                     PPALGTGAEATVTAHPQAGAKGATSKTATLAPGGPAGEFSGALALPPGTYMLRGRVAR
                     AGRLVGTDSARVAVGTQGIEYEELAADPTPLARLAERSGGTAAPLDSAGRVLDRLRSP
                     DLVRVRLAEMDLFHNPLLFLVLIAALTLEWSLRRKFHLM"
     gene            complement(4270..4926)
                     /locus_tag="VFR25_02890"
     CDS             complement(4270..4926)
                     /locus_tag="VFR25_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9940025.1"
                     /translation="MKGIDVYVEEAAQNPAVSILRVSGYVDTTTSPDLERRLQALLKE
                     KRFHIVVDLASVEYISSAGWGIFISEIREIRENGGDLKLAGMIADVREVFDLLEFENI
                     LKAYADSEQAVVSFGAVAGSPSSSKSSTPATASAPSSQPEGPQRSEVVLSDEDLVREI
                     ARQHPEFGLMRIQRELRRPERGSRDLNPVQIYVLLRKLDLDTKERREAFARSAQPTSR
                     "
     gene            complement(4923..7394)
                     /locus_tag="VFR25_02895"
     CDS             complement(4923..7394)
                     /locus_tag="VFR25_02895"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9940026.1"
                     /translation="MLSLVPRAMAPRRFNLRVAAEERHLVEIRDFVQDAGEKLLIPQK
                     TLAQTKLAVDEACTNVVRHGYKGEPGFIEIAITGNGRDFSIAIHDTGKVFDIRNVKSP
                     DLKMYVETRKRGGLGVFLMNQLMDEVRYRSSSDGNVLTMTKRLGRSRRRKGKGKQRKS
                     LRFQYTIQAFGAITLLVAVAFSTIHWRQARVIEKDAVSQAKSTASSLGQGSVEVLQRA
                     EPMSPEQTHLGETVRALLKAHPEYASARVFDARGRIWASDRFEELFTVRTLPKGVHAV
                     ASGPGAKPSPPLALVEPSDGQKREVYQPILEPGAKEPRAIGWVAIAVRESAIAEEISA
                     ARIELATIALFTLLLGCGLSAILITIFVQPIQALSDSVRAIGEGTMVADIGTSGNEEI
                     DEIARAFNEVTAKFRSAQSNLMEQERMQQEMQVAQEIQHMLLPRKVPELEGFELGYLY
                     QAAKEVGGDYYDFLTVDERTVGVVVADVSGKGVPGSLVMTMIRTALRMEARGNRSASD
                     VMARMNSFVTEDMKKGMFVTMFYVVLDSVNRVVTYASAGHNPMILYRGESDATYFLKP
                     KGIPVGINVPDEELFRKTISVEKLTLRQDDMLVIYTDGITEAMNPDREQFGEARLLTA
                     IKKHGHGTAQEFVDALNREIREFTGDAPQNDDITLVAIKEQVPVEERLEANRRELFRL
                     IDVEGVSIAEACDRLKVAPSTYYRYKRRVSDLGGEAGLKQSRPTPPLARASLEEEAAI
                     LDIVRVEPLFGAKRIHEVLHAAKRCRNDLSERAVYATLKRHGLNRKEKRLEFAQTGTD
                     TRMARLAQAIRSDEPGPTESGGGDA"
     gene            complement(7414..9288)
                     /locus_tag="VFR25_02900"
     CDS             complement(7414..9288)
                     /locus_tag="VFR25_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940027.1"
                     /translation="MRRRAILHASVAVALLLFAVARMAVAQEAGTLNLLAAPGTWPTT
                     GAVSTSTALALDPSAVYLNPAGLATQDERSLLVHHGLLQFDTSWDLAAVAYPLPGLGA
                     VGIGLARVGTSGIDAYDSNNLPLGSIGYSETALAASVSRQVYGPVTAGLTFKVLSQSL
                     GEASAAASAVDVGALFRPAMLRGGQVGLAVQNAFGGSLDLGGSAPALGRSLRVGLASP
                     EWRWGSRNALRGILDLGKQGAEPMTSRAGVEMTRVGWGSLRAGLNAGHPILGAGVTWR
                     RYGLDVAIAQGEVETTHQIALHVAWGEPVSQYEERRRAQYQKAAEESLLARSASRHAA
                     DRAKAEASEQSGDWETALVLWEVIARERPDDALVARRAAKARDEVTARARRAVEEESA
                     HRLASTLAGLMRDALGRGDLEEAEGLRRGLDLRAAGTPYADSLAQLETALDTARAREA
                     SSAAARADSLHREGRVLDAAEAAAFALRLDPNQARAKDVWTALEGTVGKSANDAAALA
                     RKLDALTAVHAASQAFNEGRYGDAQTAVRKALALDPGSKEAKAWKDRVQRRLSTPRPE
                     LDARIKQLYIRGMEAFTSGDYKEALKSWEQILVLDPLNESARRNVLEARERMKAEASR
                     "
     gene            complement(9285..>9656)
                     /locus_tag="VFR25_02905"
     CDS             complement(9285..>9656)
                     /locus_tag="VFR25_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9940028.1"
                     /translation="LTGTTSPIRGGGGLAFQPIASPALTLFVAAHGYPNPFHAGRENV
                     KLSYKLAEDASVKVTIYTLLGARVRDLTLAAGAPGGTRGLNEVAWDGRNGNGDLVLPG
                     VYVARIEGGGAAEQIKVGVLR"
BASE COUNT         1380 a         3436 c         3410 g         1430 t
ORIGIN      
        1 ggactggtag agggtcgtgt gcgatgcgga cgcggcgaat ccggcaggct cgaagaaggg
       61 gacggattcg aggtcgaggc tcgccgcgcc gagcgccgcg gggcttccgt cccgcacctc
      121 gaacgcggcg cgggctggag acgtgaatgc aagggccgcc gcgagcgtga cggcgagcgg
      181 gcgaaccgcc gcggcgctca tggcctgcct cccgcgacgg cgatcgacag cgaggagcga
      241 aggggcgcca caccgtcctc gggaagcgct tccgcgcgga cgacatagag tcccgcgggc
      301 gccggccctc ccgcgtcgtc gttgccgtcc cacacgaacg cagcgtccgt cgcgagccgc
      361 tgtccctccg tcagcgtgcg caccggccgg ccgaggaggt cgtgcacgcg cacggtgagg
      421 cgccgcccgc gcgccgcttc ggtcgcgcgg aacacgatcg gcacggaccc ccgagcgtga
      481 accgagagca cgcgcgtggc cgtgacgagc agcgcgtgtc cgcccgccga tggccccgcc
      541 ggcgcgtgga gcgagttcgc gcgtccaggc gtcgcgccgt tcgcgtcgac gcactccgac
      601 catgagcctg gatcgccgct cggaagatcg aacgagaggc gctcgagact cccgtcgcgc
      661 acggtggctc cgccggcgta ggtcatggcg tcgaccggaa cgccgtccgc gagcgcgagc
      721 gcgaccatgt ccgcggggct gcctggctcc gcgccgtcgt tgagcgaggg ccatccgccg
      781 gtcacgccga ccacgatgct gtcgggaagc ccgcaccggc tccgcaccgc cgcgggatcc
      841 tgagcgacga cgaggtagga gcccgccgcc agcgagcgcg gggccgagcc tcgatcgatg
      901 gggcgagggc tggatgccgc gtcggccagc gtgagcgagc ccacgtcctc gatcggctcg
      961 cgcgcccaga tctcgatcca ttccccggag cccgtgttat ggaacgcgat ctcgctcagc
     1021 acgacggggg acgcgatcga acggagccag gcggccacgg tgtcgctcag agccgcgtcc
     1081 tcacccagcg cgccctcgag ccgcagtctc acccgacgcg cccccggcgc ggggtccacg
     1141 agtgagagcg agaccgaagc gctctctccg ggtgccaggt ccacgcctgg ggcggccgcg
     1201 cccggcaccc acgatcccga agcttcgaga tcgcaggcga tccgccatcg ggccccgaag
     1261 agcggaagcc gtcccgagct ccgcacgctc gcgcgtgcca cgaagggctc gcccggccag
     1321 cacacggacg gagacagcac gagcttcgcg cgaccgaagc gaagccgcat gtcgggatgg
     1381 ttggcggcgc cgggcgtggg ctccgctgcc acggcccagt cgtcccggtt caactcggtg
     1441 tccacgccgt cctcgacgcg cgccacgctc gcgcccgatg gcgcatcggg agcgggcgag
     1501 ccttcgtact ggccggggtc gctcagctcg ccgtagccga ggaggtcgag cacggcgccg
     1561 gcgcgcgtga gccggatcgc gtcgggaccg ttctggagcg cggcgaggag ccgcgcggcc
     1621 ccgacgacga acggcgagcg cggcccggcg atatcccccg cgttcgcgcg ccacaccacc
     1681 gcccacgtgc cggggcgcgc accgtccgac gtctccagcg cgacgccgtc gagcggcacc
     1741 gcgaccgaat ccggattcca gagctcgacg aactcgtgcc cttcgtcggc gccatcggga
     1801 tcgtagagca cttcgttgag gacgaggtcc gcgcgcgcgg tggacgcgac ggcgagcgcg
     1861 cacgcgccaa cgagcgcgag cttgagcatg gcttcctcct tccggaggag gcgcggggga
     1921 tgctggtgtt gagaggtggg cgaggtcggg aagccccgtt acatcagatg aaatttcctc
     1981 ctgagcgacc actccagcgt gagcgcggcg atcagcacca ggaacagaag cgggttgtgg
     2041 aacagatcca tttccgcgag ccgcacgcgc acgaggtcgg gagagcggag gcgatcgagg
     2101 acgcgaccgg cgctgtcgag cggtgcggcg gtgccgccgg accgctccgc gagacgcgcg
     2161 agcggtgtcg gatcggccgc cagctcctcg tactcgatcc cctgcgtgcc gaccgcgacg
     2221 cgagcgctgt cggtcccgac gaggcggccg gcgcgggcga ctcgcccgcg gagcatgtac
     2281 gtcccgggag gaagggcgag cgcacccgag aactcgccgg ccggcccgcc aggcgcgagc
     2341 gtcgcggtct tgctcgtcgc gcccttcgcg ccggcctgtg gatgcgcggt gaccgtggct
     2401 tccgcgcccg tgccgagcgc gggaggcgtg gtggcgacgc ggaccgcgat cggccggccg
     2461 acgggcgtga tgtcgggcgc ggtgatcgag accggacgat cctcccatcc gcccgcaagc
     2521 catcgctcgg cgccgccgaa aaacgacccc tcgatcccag gcttgccggt gagtcccgcc
     2581 gccgtgaggc cccaccggta cacgccggcg gcattcagga ggacgacgcg ccccttccca
     2641 accgaaccgg cgaggacgat cggaatcgct tcgttgccga cgcggccgat cacgatggtc
     2701 tggagcgggc tcccggcggt tcccagcgac agaatcggct cgaccggcgg aaggtccttc
     2761 cagatcgagg ccgcgctcgc tgcgtcgccg gaaaggagag ccacatcctg cgtgcgcccc
     2821 gcgggcgcga gctccgtgct cgcgatcggg ccgagcggct gcccgaagag ccgccactgg
     2881 atgctgagcg cgcgcgtgag cgccgattcg actggcggcc gcggcgtggg gtcgatccac
     2941 aggagcacgc cgccgccgcg cgcgaggaag cgcatcagcg cgtccgcgcg ctcgggggtc
     3001 atcgtgccgg agtcgtagcg aaccaccacc gcctccgcat cctcgagcca gctcgagaac
     3061 ggccgcggcg ctcctcccag cgcgactgcc tgcgaggagc ccgacggcgt gaggcgagcc
     3121 accgcgcgcg tcgtgtcctc ttcgatgcca cgcgcgagcg agcggaggtc ccagtcccag
     3181 ctcgacgcga ggaccgggat cgtgcgtttg gcgggacgcg ccaagacggc gacgaggcgc
     3241 tgcttgttct cgcgcatcgg atcgtcgggg acgtcgacca ggtcgaccgt gaggaatcgc
     3301 ttgccgcgct cgaggagcgt caccggaatc gaaacacggg cggacgcgcc ggggccgtgg
     3361 agcgcgaatt ccgtgcgccc gagcacgcgc ccttcctcgc tcacgcggac cgtgccgcgc
     3421 gattcctgcc tggacccctg gcgcacgctc acgacgagcg gcgtcgggcg acccaggatg
     3481 atctcgggag gcgtttcgac gcccgcgatt cccaccgtgg gtggatccgt gtggcgcccg
     3541 accgtgaggc cgaagagcgg gaccgcggcg ccgagcgcct gctcgggatc ctcgcctcgg
     3601 ttcacgacgc cgtcgctcag cacgagcatc gcggcgaccg agtcgggatc cacgcggttg
     3661 aaagagaatc ggatggcgtc gccgagcgcg gtctcgcccc cgccctggaa ggcgagcgcc
     3721 ttggatgcgt ccttgtcgta cgggccgagc gccgcgtcga aggacgcggt ccggacctcg
     3781 tagcgaccgc cgaggtcgcg cgcgaccgcc tgcgcggcgc tgtccgccgc ggcgcgacgg
     3841 ctccgtccgc cggagagatc gcggagatcc atgctccccg aatggtcgac caggatgagg
     3901 agccggtttc gcgcgcgcga cgtctccgga tagcggagca cgggcgcgat cgacgccacg
     3961 aggagtgcga ggagtgcgag cagccgcgtc gctaggagta tgccgcgtcg cttcgccggg
     4021 accgccggat agcgcgtgag gtaggcccag agcccgatcg cgatcgccgc gccgatcaag
     4081 gcgagcgcga cgagctcagg cgtcgcttgc agttcgatct tcacgcggag aacgctcccg
     4141 gcgccatgga tggaccagtg gactgacgat cattcgggat ctgtgcgccg aggaggcggg
     4201 caacagttcg gggggccgtg ccgaaaacga taggcagcgg ccccccgaag ggtcaagcgg
     4261 gaacttggac tagcggctgg tcggctgcgc gctccgcgcg aaggcttcgc gacgctcctt
     4321 cgtgtcgagg tcgagcttcc ggaggaggac gtagatctgc accggattga gatcgcggct
     4381 gccacgctcc gggcggcgca gctcgcgctg gatgcgcatg aggccgaact cggggtgctg
     4441 gcgcgcgatc tcgcggacca gatcctcgtc ggagaggacc acctcggagc gctgcggccc
     4501 ttccggctgc gagctcggcg cgctcgcggt cgcgggcgtc gaagacttcg acgaggacgg
     4561 cgatccggcc accgcgccga aggagacgac cgcctgctcg gagtcggcgt acgctttgag
     4621 aatgttctcg aattcgagaa ggtcgaacac ttcgcgcacg tccgcgatca tgccggcgag
     4681 cttcaggtcg ccgccgttct cgcggatctc gcggatctcg ctgatgaaga tgccccagcc
     4741 cgcgctcgag atgtactcga cgctggcgag atcgaccacg atgtggaagc gcttctcctt
     4801 gagcagcgcc tgcaggcggc gctcgaggtc ggggctggtg gtcgtgtcca cgtagcccga
     4861 cacgcgcagg atcgaaaccg ccggattctg cgccgcttcc tcgacgtaaa cgtcgatccc
     4921 cttcatgcgt cccctcctcc tgattccgtg gggcccggct catcggacct gattgcctga
     4981 gcgagcctcg ccatccgtgt atcggttccg gtctgggcga actccagcct tttctcctta
     5041 cgattgaggc cgtgacgctt cagcgtggcg tagacggccc gctcggaaag atcgttcctg
     5101 caacgctttg cagcgtgcag aacttcgtgg atccgcttcg cgccgaagag aggctcgacc
     5161 cggacgatgt ccaggatcgc ggcctcctct tcgaggctcg cgcgggccag gggcggggtc
     5221 gggcggctct gtttgaggcc ggcctcgcct cccaggtcgg acacgcggcg cttgtagcgg
     5281 tagtaggtcg agggggccac cttgagccgg tcgcacgcct cggcaatcga gacgccctcg
     5341 acgtcgatga gccggaagag ctcgcggcgg ttcgcctcga gtcgctcctc caccggcacc
     5401 tgctccttga tcgcgaccag cgtgatgtcg tcgttctgcg gcgcgtctcc cgtgaactcg
     5461 cggatctccc ggttgagcgc gtccacgaat tcctgcgccg ttccgtggcc gtgcttcttg
     5521 atcgcggtca agagcctcgc ctccccgaat tgctcccgat ccggattcat cgcctcggtg
     5581 atcccgtccg tgtagatcac gagcatgtcg tcctgacgca gcgtgagctt ctccacgctg
     5641 atcgtcttcc ggaagagctc ctcgtcgggc acgttgatcc cgaccggaat tcccttcggc
     5701 ttcaagaagt aggtggcgtc cgactcgcca cggtagagga tcatcggatt gtgcccagcg
     5761 ctcgcgtagg tcacgacgcg gttcacggag tcgagcacga cgtagaacat cgtgacgaac
     5821 atccccttct tcatgtcctc ggtgacgaag gagttcatcc tggccatcac gtccgaggcc
     5881 gagcggttgc cgcgcgcttc catgcggagc gcggtccgga tcatggtcat gacgagcgag
     5941 cccgggacgc ccttgcccga cacgtccgcg acgacgacgc ccacggtccg ctcgtcgacg
     6001 gtgaggaagt cgtagtagtc gccgccgact tccttcgcgg cctggtagag atagcccagc
     6061 tcgaagccct ccagctccgg caccttgcgc ggcaggagca tgtgctggat ctcctgcgcg
     6121 acctgcatct cctgctgcat ccgctcctgc tccatgagat tgctctgcgc cgagcggaac
     6181 ttggcggtca cctcgttgaa cgcgcgtgcg atctcgtcga tctcctcgtt ccccgacgtg
     6241 ccgatgtcgg cgaccatcgt cccctcgccg atggcgcgca cgctgtccga gagcgcctgg
     6301 atcggctgga cgaagatcgt gatcaggatc gccgagagcc cgcatccgag gaggagcgtg
     6361 aagagggcga tggtcgcgag ctcgatgcgc gcggcgctga tctcctcggc gatggcggac
     6421 tcgcgcaccg cgatcgccac ccatccgatg gcgcgcggct ccttcgctcc cggctcgagg
     6481 atcggctggt agacctcgcg cttctggccg tccgagggct cgacgagcgc caggggaggc
     6541 gacggcttcg cgcccggccc gctcgcgacg gcgtgcactc ccttcggaag cgtgcgcacc
     6601 gtgaagagct cctcgaacct gtccgacgcc cagatgcgcc cccgggcgtc gaacacgcgg
     6661 gcggacgcgt actcggggtg cgccttcagg agcgcgcgca cggtctcgcc cagatgcgtc
     6721 tgctccgggg acatcggctc ggctctctgg agcacctcga ccgacccttg gccgaggctc
     6781 gaggcggtgc tcttcgcctg gctcacggcg tccttctcga tcacgcgcgc ctggcgccag
     6841 tggatggtcg agaaggcaac cgccacgagg agcgtgatcg cgccgaacgc ctggatcgtg
     6901 tactggaagc ggaggctctt gcgctgtttt cccttcccct tccgccggcg cgagcggccg
     6961 agcctcttcg tcatggtgag gacgtttccg tccgagctgg agcggtagcg gacctcgtcc
     7021 atgagctggt tcatcaggaa gacgccgagg ccgccccgct tcctcgtctc gacgtacatc
     7081 ttgagatccg ggctcttcac gttccggatg tcgaagacct tgccggtgtc gtggatcgcg
     7141 atcgagaagt cgcggccgtt tccggtgatc gcgatctcga tgaagccggg ctcgcccttg
     7201 tagccgtggc gcacgacgtt cgtgcacgcc tcgtccacgg cgagcttggt ctgtgcgagc
     7261 gtcttctgcg ggatgaggag cttttccccc gcgtcctgga cgaagtcgcg gatctcgacc
     7321 aggtggcgct cctcggcggc cacgcggaga ttgaagcgcc ggggcgccat cgccctcggg
     7381 acgaggctca gcatcgcgcc ctcccacagc ccctcatcgg ctggcctccg ccttcatgcg
     7441 ctcgcgcgcc tcgagcacgt tccgccgcgc gctctcgttc agaggatcga gcacgaggat
     7501 ctgctcccag ctcttgagcg cttccttgta atcgccggag gtgaacgcct ccatcccgcg
     7561 gatgtagagc tgcttgatgc gcgcgtcgag ctcggggcgc ggcgtggaca ggcggcgctg
     7621 gacgcggtcc ttccaggcct ttgcctcctt gctccccggg tcgagcgcga gcgccttccg
     7681 gaccgcggtc tgcgcgtcgc cgtaacgccc ctcgttgaac gcctgggacg cggcgtgcac
     7741 ggccgtgagc gcgtcgagct tccgcgcgag cgcggccgcg tcgttcgcgc tctttccgac
     7801 cgtgccctcg agcgccgtcc acacgtcctt ggcccgcgcc tggttcggat cgaggcggag
     7861 cgcgaacgcg gctgcctcgg ccgcatcgag gacgcgcccc tcacgatgca gcgagtccgc
     7921 gcgcgcggcg gcgctcgagg cctcccgcgc gcgggccgtg tcgagcgccg tctcgagctg
     7981 ggcgaggctg tccgcgtacg gggtcccggc ggcgcggaga tcgaggccgc ggcggagtcc
     8041 ctccgcttcc tccaggtcgc cgcgaccgag cgcgtcgcgc atcaggcctg cgagcgtcga
     8101 cgcgagacgg tgcgcgctct cctcctccac cgcgcggcgc gcgcgcgccg tgacctcgtc
     8161 gcgcgccttc gcggcgcggc gcgccaccaa ggcatcgtcg ggacgctcgc gggcgatcac
     8221 ttcccagagg acgagcgcgg tctcccagtc gccgctctgc tcggatgcct cggccttcgc
     8281 gcgatccgcc gcgtggcgcg aggcgctccg cgccaggagc gactcctcgg ccgccttctg
     8341 gtactgggcg cgccgtcgct cctcgtactg gctcaccggc tcgccccacg cgacgtggag
     8401 cgcgatctga tgggtggtct cgacctcgcc ctgcgcgatg gcgacgtcga ggccgtagcg
     8461 cctccacgtc actcccgctc cgagaatggg atggcccgcg ttgagacccg cgcgaagcga
     8521 tccccagccc acgcgcgtca tctccacgcc cgcgcgcgac gtcatgggct cggctccctg
     8581 cttcccgagg tcgaggatgc cgcgaagcgc gttccgcgac ccccagcgcc actcggggct
     8641 cgcgagaccg acgcggagcg agcggccgag cgcgggcgcc gagcctccca gatcgagcga
     8701 gccgccgaac gcgttctgga cggccagtcc gacctgtcca ccccggagca tcgcgggccg
     8761 gaagagcgcg ccgacatcga ccgccgacgc cgcggccgac gcctcgccga gcgactgact
     8821 cagcaccttg aacgtgagtc ccgcggtcac cgggccgtag acctggcgcg acacgctcgc
     8881 cgcgagcgcc gtctcggagt agccgatcga tccgagcggc aggttgttcg agtcgtacgc
     8941 gtcgatgccc gacgtgccga cccgcgcgag cccgatcccg accgcgccga ggccgggcag
     9001 cgggtaggcc accgcggcga ggtcccagct cgtgtcgaac tggaggagcc catggtggac
     9061 gaggagcgag cgctcgtcct gggtcgcgag acccgccgga ttgaggtaga ccgcggacgg
     9121 gtccaaggcg agcgccgtgc tcgtgctcac cgcccccgtc gtgggccagg tgcccggagc
     9181 cgcgaggaga ttgagcgtgc ccgcttcctg cgcgacggcc atccgcgcga cggcgaagag
     9241 aaggagcgcg acggcgacgc tcgcgtggag gatcgcgcgg cgcctcatcg cagcaccccc
     9301 accttgatct gctccgccgc accgccccct tcgatgcgag cgacgtacac gcccggaagc
     9361 acgagatcgc cgtttccgtt ccggccgtcc cacgccacct cgttcaaccc gcgcgttccc
     9421 cccggcgcgc cggcggcgag cgtgaggtcg cgcacgcggg cgccgaggag cgtgtagatc
     9481 gtgaccttga cggaggcgtc ctccgcgagc ttgtaggaga gcttcacgtt ctcgcgcccc
     9541 gcgtggaacg gattcggata gccgtgcgcg gcgacgaaga gggtgagcgc cggcgacgcg
     9601 atgggctgga acgcgagccc cccgcctccg cggatggggg acgtcgttcc ggtcag
//
LOCUS       DASTKC010000065        10894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1940770, whole genome shotgun sequence.
ACCESSION   DASTKC010000065 DASTKC010000000
VERSION     DASTKC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940770"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFR25_02910"
     CDS             complement(<1..322)
                     /locus_tag="VFR25_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940029.1"
                     /translation="MNPPNILRSSVVAIALLVVAAALQGAPAQPSASAKPSAKPPSKP
                     KSTAPAPAPRPDEIGMVDGIPIYQIDWDRLAEPYFEEIEAKAGRKLSDDDKKLLRKNV
                     LDELI"
     gene            complement(784..2211)
                     /locus_tag="VFR25_02915"
     CDS             complement(784..2211)
                     /locus_tag="VFR25_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9940030.1"
                     /translation="MSSRTRPTLLVVDDDSGSSGLLREVFVQEGYDVSVAQSGAEALK
                     QAAERPFDVVLSDIQMPDIDGIEVLRRLRKVAPDATVILVTAYSTIEMAIRALNEGAF
                     DYVRKPFKLDEVRRVVERAMERRRAREQTTKAGAAPAPTSSAGEPRRRGRPISAIIGS
                     HPDMVELFKLVSRVAGTKSSVMIQGESGTGKELIARTIHEASTRRDRPFVAVNCTSLS
                     ETLLESELFGHVKGAFTGAIERRPGLFLEANRGTVFLDEVGDMSLSMQAKLLRVLQEE
                     EVKPVGGTDTIPVDVRVVAATHQDLEALVRTGRFRLDLYYRLHVVALRVPPLRERRDD
                     IPLLAEHFLREYGLLAQRPLQGFSTKAMRALMNYPWPGNVRELENVVEGAVALAPGSI
                     VEESDLPDKIVHHAGPVPATPGGQATLDEMTRRYVLSVLDKVRGNKTEAARILGVPRR
                     TLYRMLERYGVGTEVEVSQSGTSIH"
     gene            2419..3753
                     /gene="mgtE"
                     /locus_tag="VFR25_02920"
     CDS             2419..3753
                     /gene="mgtE"
                     /locus_tag="VFR25_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9940031.1"
                     /translation="MTSSPEPELEEPLSTADLQEAWSLLVPSDRLESFRALERTEAED
                     FFLSLSARDQAELLLGIPSAERRSWMRLLAPDDAADLVQELEPEQREELLQLLDDPTR
                     SDVSGLLAYAEDDAGGLMNPRYARLRPDMSVDEAITYLRRHAAERAETLTYAYVLAHD
                     NKLLGVVSYRDLITAKTDQKIDSIMRTDLVTVPEAMDQEEVSRLFATYRLMAFPVVDS
                     EGRMKGIVTLDDIVDVVREEATEDIQKIGGTAVLDAPYLRMSTIDMVKKRAGWLSVLF
                     LGESMTAATMGYFEGEIARAVVLALFVPLVISSGGNSGGQATTLVIRAMALGEVKLRD
                     WWHVMRREIVSGLLLGGILAAIGIARITIWQALFHSYGPHYFLIAICVALSLIGVVLW
                     GTLVGSMLPFILRRLGLDPATASAPFVATLVDVTGLIIYFTAARVILSGTLL"
     gene            complement(3766..5346)
                     /locus_tag="VFR25_02925"
     CDS             complement(3766..5346)
                     /locus_tag="VFR25_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017188890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET9940032.1"
                     /translation="MGIALAAILLLWSTSAIAQEKKPNILVIMGDDIGWFNPSCYNSG
                     IMGYSTPNIDRIAAEGARFTDWYGQQSCTAGRAAFVTGQSPIRTGLTKVGLPGAPIGL
                     SAKDPSMAEFMKQLGYTTGQFGKNHLGDLNQFLPTVHGFDEFFGNLYHLNAEEEPEDA
                     DYPKDPKFKAKFGPRGVLHCWATDKDNATVDPTYGRVGKQKIENTGPLTVKRMETVDD
                     EFTDAALGWMDKQAKAGKPFFCWYNSTRMHIFTHLKKESQGVTGLGIYPDGMVEHDAH
                     VGQLLRKLDDLGIANNTIVVYTTDNGAEVFTWPDGGTTPFKGEKATNWEGAFRVPMLI
                     RWPGVIHPGTVINDICAHEDLVPTFAAVAGEPDLVAKVLKGYRAGGKSFKVHLDGYNL
                     VPFLKGEVKESPRKEFIYWSDDGEAMAIRYQNWKVAFMEQHTETSPQTPIGVWQGEFT
                     RLRAPNLYNLRADPFEHGPEAVGFAEWQMHHTFLIVPAQAYVAQWLQSFKEFPPRQKP
                     ASFNLDDVMRKLSEGGGQ"
     gene            complement(5569..6513)
                     /gene="lipA"
                     /locus_tag="VFR25_02930"
     CDS             complement(5569..6513)
                     /gene="lipA"
                     /locus_tag="VFR25_02930"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9940033.1"
                     /translation="MDSDRRSQAGEKRKPLRQYQPLTLTPPGPRRPDWLRARIPSGKE
                     YQETKAILRTLDLHTVCESANCPNIGDCFSRHTATFLILGNVCTRACPFCDIRSGKPL
                     PVDPDEPARVADAVRRLGLHYTVVTSVNRDELPDGGAEHFAQTIRAIHEAIPSAKVEV
                     LIPDFLGDEAALRTVLEARPEVLNHNLETVKRLYKRVRPAGRYDRSLELLKRVASWEP
                     AIPAKSGIMVGIGETVEEVEELLRDIHAQGVSMATIGQYLPPSKNHLPLERYVTPDEF
                     AHYREYGLGLGIRQVASGPLVRSSYHAEEQAGETVFIP"
     gene            complement(6539..7936)
                     /gene="lpdA"
                     /locus_tag="VFR25_02935"
     CDS             complement(6539..7936)
                     /gene="lpdA"
                     /locus_tag="VFR25_02935"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9940034.1"
                     /translation="MSPDRYDVAILGGGPGGYVAAIRAGQLGLKTVVIEKDKVGGTCL
                     HVGCIPTKALLEAAEVLLHCKRAGEFGIKVAEATLDLKAAMERKDRVVKKNFMGTEGL
                     LRKHKVTTLKGTGKLRSAKSIEVKGADGSVQTIEAGAIVLATGSKVGSLPMAPVDGRV
                     VISSDDILSLDRVPESLLVIGAGAVGVEFASIYHAFGSKIVLIERMPRLVPIEDEDVS
                     QALLRSFTRQGMEIHVGADLKSVRAEGDAAWSEFVVGGETKRVRTERVLMAAGRKPVT
                     DGIGLETVGVKMDRGYVLVNENMETNVAGVYAIGDIVPTAALAHVASHEGVVAVERIA
                     GKKAHPVNYAAIPNCTYCHPQVASVGLTEAQARSTGREIKTGKFPFTANAKASILGEG
                     DGFVKSVADAKTGEILGVHIIGVLATEQIAESVVARHFEASALELADVVHAHPTLAEA
                     TAEAMLGTDGRTIHI"
     gene            complement(7933..8487)
                     /locus_tag="VFR25_02940"
     CDS             complement(7933..8487)
                     /locus_tag="VFR25_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9940035.1"
                     /translation="MNPVTASAPFLDLATAMPGPLPTEAQRMLAPNVAAMIGRGEPHA
                     EVLAHLVRAVHEAFPAYAWTGIYLARDGMLEMGPFHGPDSPHHKIRIGTAEGGDTQGI
                     CGWVAAHGVPQIIPDVNADPRYLMCSASIRSEIVVPIERDGRVLGVIDIDSETPALFT
                     QTDLTILTDLARLIAPTFPVEPRP"
     gene            complement(8484..9290)
                     /locus_tag="VFR25_02945"
     CDS             complement(8484..9290)
                     /locus_tag="VFR25_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET9940036.1"
                     /translation="MFSYTAITLVVLGILILSSAIKVLREYERAVIFRLGRSIGMKGP
                     GLILLIPIIDKMVRISLRTVAMDVPPQDVITRDNVSVKVNAVVYFRVMDPVRAVVEVE
                     NFLYATSQMAQTTLRSVLGESDLDELLSKREKINKELQRVIDGHTEPWGVKVSTVEVK
                     NVDLPQEMQRAIARQAEAERERRAKIINAEGELQASQKLTEAAAVIATQPLAITLRYL
                     QTLSEIATENNSTTLFPIPIDLFEPLIRAKDRAMAAIPPPPPTALPGRTS"
     gene            complement(9302..10627)
                     /locus_tag="VFR25_02950"
     CDS             complement(9302..10627)
                     /locus_tag="VFR25_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET9940037.1"
                     /translation="MKRLAARGILSLALLIAIIAAVSASAKDPADSTRAGSDRPQVDV
                     LTWEGPVQPIMAETVIQAIDRAEKAHREALVIRLDTPGGLDTSMRDIIKRILISEVPI
                     VVYVAPSGGRAASAGTFIGMASHVLAMSPGTSIGAATPVNVGGQDVAKDMANKVKNDA
                     VSYIRSLARQRGRNADWAEKAVREGSSLNEEDALHEHVIDLVARDLTELLKAIDGRTV
                     SVAGTTRTLHTANAETHAYEASWRQKFLGRIIDPNIAYILFILGFYGLLFELSNPGAV
                     LPGVVGGICLLLAFLAFQALPVNLTGVALIVFAMVLFAADVKVQSHGILTVGGAAALL
                     LGSLILLGGDKGVARISLSVILTAVGATVAFFALVIGAGLRAQRLKPTTGTHGMVGEQ
                     GTALTDLGPSGQVFVHGEYWAAEADQPIAKGARVVVHRVDGLVLRVKKA"
     gene            complement(10624..>10894)
                     /locus_tag="VFR25_02955"
     CDS             complement(10624..>10894)
                     /locus_tag="VFR25_02955"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019392761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET9940038.1"
                     /translation="LPWEQVNVLNVNNGERFDTYAIRGPRGSGVICLNGPAARLAHVG
                     DLVIILTYAQMEREELLRHTPIVVHVDENNRIQTSSAGSNRGAKG"
BASE COUNT         1699 a         3648 c         3647 g         1900 t
ORIGIN      
        1 ggatcagctc gtcgaggacg ttctttcgga ggagcttctt gtcgtcgtcg ctcagcttgc
       61 gccccgcctt ggcctcgatt tcctcgaagt acggctcggc caggcggtcc cagtcgatct
      121 ggtagatggg gatgccatcc accatgccga tctcgtccgg acgcggagcg ggagccggcg
      181 ccgtggactt cggtttggac gggggcttcg cggatggctt cgccgaagcc gacggctgcg
      241 cgggcgcgcc ttggagcgcg gcggcgacga cgagcagggc gatggcgacg acggacgatc
      301 gcagaatgtt gggggggttc actgggacgg aaagtctaga aacgggtcca ggtcctgtcc
      361 acgcgaaacg ccggttgacg gcaaagaaaa gcccctggag ggcggttctc gcgaagtgtg
      421 gggggcagaa gcatcgcgag gctgccgcca gctaccaggg gccttaaacc gtcgacttga
      481 acccaaacgc gacccgatac gctgaagcag ggcaagatgt cgtgcacacg cgggcgagcc
      541 acgctgggtc ccagccgtga ggtctgggta cggtctagcc cttttgcact caccgtgcca
      601 ggtcagcggg cacgtcgatc gttttggttc ttgggttaag ttcatgtaaa aatgacagtt
      661 acactcaagg gctggatcga cctcaggacg ggaccgacgg ggttcttgtg ccgcaggata
      721 ccgagggtgt ggatgagtcg ttctaagcca aactcaaaga atcagttatc agacgtgagt
      781 caatcaatgg atcgatgtgc cggattgact cacttccact tcggttccga caccgtaacg
      841 ttccagcatc cggtacagcg ttcggcgcgg aaccccgagg atccgggcgg cttccgtctt
      901 gttcccccgg accttgtcga ggaccgaaag aacatatcgc cgcgtcattt cgtccagagt
      961 ggcctggccc cccggggttg ccggcaccgg gccagcgtga tggacgatct tgtcgggcag
     1021 gtccgactcc tcgacgatcg accctggcgc gagtgcaacc gcgccctcca ccacgttctc
     1081 gagctcccgg acgttcccgg gccaggggta gttcatcagg gcgcgcatcg ccttggtgga
     1141 gaacccctgg agcggacgct gcgcgaggag tccgtactcg cggaggaagt gctcggccag
     1201 gagcgggatg tcgtcccgcc gctcgcgaag cggcggcacg cgcagcgcga ccacgtggag
     1261 ccggtagtag aggtcgaggc ggaagcgtcc cgtgcggacg agcgcctcga gatcctggtg
     1321 cgtcgccgcg accacgcgca cgtcgaccgg aatcgtgtcc gtgcctccga ccggcttcac
     1381 ttcctcctcc tggagcacgc gcaggagctt cgcctgcatg gagagggaca tgtcgccgac
     1441 ctcgtctagg aagacggtcc cgcggttggc ctcgaggaag aggcccgggc gacgctcgat
     1501 ggcgccggtg aaggctccct tcacgtgccc gaagagctcc gactccaaga gcgtctccga
     1561 gaggctcgtg cagttgaccg cgacgaacgg gcggtcccgg cgcgtcgagg cctcgtggat
     1621 cgtccgcgcg atcagctcct tccccgtgcc gctctcgccc tggatcatga ccgagctctt
     1681 cgttcccgcg acgcgggaga cgagcttgaa gagctcgacc atgtccggat gcgatccgat
     1741 gatcgcggag atcgggcgcc cccgccgtct cggctctccc gcggacgacg tcggagccgg
     1801 cgccgcgcca gccttggtcg tttgctcccg cgcgcggcgg cgctccatgg cgcgctccac
     1861 cacccgccgc acctcgtcga gcttgaaggg cttccgcacg tagtcgaagg ccccctcgtt
     1921 gagcgcgcgg atcgccatct cgatcgtgct gtaggccgtg acgaggatca cggtcgcgtc
     1981 gggcgccacc ttgcggagcc ggcgcagcac ctcgatcccg tcgatatcgg gcatctggat
     2041 gtcgctcagc acgacgtcga agggacgctc ggccgcctgc ttcagcgctt cggcgccgct
     2101 ctgtgccacg gaaacgtcgt agccctcctg cacgaagact tcgcgcaaaa gaccggacga
     2161 tccggaatcg tcgtcgacga ccagcagcgt gggacgggta cgagatgaca tcggttttag
     2221 cccccaggta ccggcgcggg gagcgccgct gccctattgt accccacctg agcctcctgt
     2281 gcgccgggcc ctgagacctg ctccatcagg aaaccggcac ccggaaggcc gctccagggt
     2341 tgctgatgga aggagcggcc ggtaccatcg gcgctcatgc ccggcgagcg ggcacccctt
     2401 cacgacgcgg aggcggccat gacgtccagc ccggaacccg agctcgagga accgctctcg
     2461 acggcggatc tccaggaagc ttggtcgctc ctcgttccct cggatcgcct cgagagcttc
     2521 cgcgcgctcg agcggaccga agccgaagac ttcttcctca gcctgagtgc tcgcgaccag
     2581 gccgagctcc tcctcgggat cccctcggcc gagcgccgct cgtggatgcg cctcctcgcc
     2641 cccgacgacg ccgcggactt ggtgcaggag ctcgagcccg agcagcgcga agagctcctc
     2701 cagcttctcg acgatccgac ccgctcggac gtgagcggtc tcctcgcgta cgcggaggac
     2761 gacgcgggcg ggctcatgaa cccgcgctac gcgcggctcc gccccgacat gtcggtggac
     2821 gaggcgatca cgtatctcag gcgtcacgcc gccgagcgcg ccgagactct cacctacgcg
     2881 tacgtgctcg cgcacgacaa caagctcctc ggcgtggtca gctatcgcga tctcatcacc
     2941 gcgaagaccg accagaagat cgactccatc atgcgcaccg acctcgtcac ggtgccggag
     3001 gcgatggacc aggaggaagt gagccggctc ttcgcgacct atcgactgat ggcgttcccg
     3061 gtcgtggatt ccgaggggcg gatgaagggc atcgtcaccc tcgacgacat cgtcgacgtc
     3121 gtgcgcgagg aagcgaccga ggacatccag aagatcggcg gtaccgcggt gctcgacgcg
     3181 ccgtatctcc gcatgagcac gatcgacatg gtcaagaagc gcgccggatg gctgagcgtg
     3241 ctcttcctag gcgagagcat gaccgcggcc accatgggct acttcgaagg ggagatcgcg
     3301 cgggcggtgg tgctcgcgct cttcgtcccg ctcgtcatct cgagcggagg aaactcggga
     3361 ggccaggcga cgacgcttgt gatccgcgcc atggcgctcg gcgaggtaaa gctccgcgac
     3421 tggtggcacg tgatgcgccg ggagatcgtc tcgggactcc tactcggcgg catcctggcc
     3481 gcgatcggga tcgcgcggat cacgatctgg caggcactct tccactccta cggcccccac
     3541 tacttcctga tcgcgatctg cgtcgccctg agcctcatcg gcgtggtgct ctggggcacg
     3601 ctcgtgggat cgatgctccc cttcatcctc cgccgcctcg gcctcgaccc cgccacggcg
     3661 tcggcgccct tcgtggcgac gctcgtcgac gtgacggggc tcatcatcta cttcaccgcg
     3721 gcgcgggtca tcctctcggg tacgctgctc tagctctcgc cgcgcctact gtccgccacc
     3781 ctcggacagc ttccgcatca catcgtcgag gttgaagctg gccggcttct gccgcggcgg
     3841 gaactccttg aagctctgga gccactgggc gacgtacgcc tgagcgggaa cgatcaggaa
     3901 ggtatggtgc atctgccatt cggcgaaccc gacggcttcc ggtccgtgct cgaacgggtc
     3961 ggcccgcagg ttgtagaggt tcggcgcgcg cagcctcgtg aactccccct gccacacgcc
     4021 gatcggcgtc tgcggactcg tctcggtgtg ctgctccatg aacgcgacct tccagttctg
     4081 ataccggatg gccatggctt cgccgtcgtc gctccagtag atgaattcct tccggggcga
     4141 ctccttgacc tcgcctttca ggaacggcac gaggttgtac ccgtcgagat gcaccttgaa
     4201 gctcttgcct cccgcacgat agcccttcaa taccttcgcg actaagtccg gctcgccggc
     4261 aacggccgcg aacgtgggga ccagatcctc gtgcgcgcag atgtcgttga tcacggtgcc
     4321 cggatggatc acgccgggcc accggatcag catcggcacg cggaatgccc cttcccagtt
     4381 ggtggccttc tcgcccttga acggcgtggt accgccgtcg ggccacgtga agacctcggc
     4441 gccgttgtcg gtcgtgtaga ccacgatcgt gttgttcgcg atgccgaggt cgtcgagctt
     4501 gcggaggagc tggccgacgt gcgcatcgtg ctcgaccatg ccgtcgggat agatcccgag
     4561 ccccgtcacg ccctgggatt ccttcttcag atgcgtgaag atgtgcatgc gcgtcgagtt
     4621 gtaccagcag aagaaaggct tcccggcctt ggcctgcttg tccatccacc cgagggccgc
     4681 gtcggtgaat tcgtcgtcga ccgtctccat gcgcttcacc gtcagcgggc cggtgttctc
     4741 gatcttctgc ttgcccacgc gaccataggt tggatccacg gtcgcgttgt ccttgtcggt
     4801 ggcccagcag tggagcacgc cgcgcgggcc gaacttggcc ttgaatttcg gatccttggg
     4861 atagtccgcg tcctccggct cctcctcggc gttcagatgg tacagattgc cgaagaactc
     4921 atcgaagccg tgcaccgtgg gcaggaactg gttgagatcg ccgaggtggt tcttgccgaa
     4981 ttgcccggtc gtgtagccga gctgcttcat gaactcggcc atgctcgggt ccttcgcgct
     5041 caatccgatc ggggcgccgg gaaggccgac cttcgtgagg ccggtccgga tcggggactg
     5101 ccccgtcacg aaggcggcgc gccccgcggt gcagctctgc tgcccatacc agtcggtgaa
     5161 gcgcgcgcct tccgccgcga tgcggtcaat gttgggcgtg ctgtaaccca tgatcccgga
     5221 gttgtagcag ctcgggttaa accacccgat gtcgtcgccc atgatcacga ggatgttggg
     5281 cttcttctcc tgcgcgatcg cggaggtcga ccacagcaga aggatggcgg ccaaagcgat
     5341 tccaatgcgt ctcacccctt tgctcctttc gccgttcgat gcacttctcg ctccagggag
     5401 ctggagcccg aatacgcatg aaagcgacca aggcgaggtg tcgggcgagg tggtggtcgc
     5461 aacgatgagg ctacgctgac gaacctggga tttggccagt atcggcgcgc tcgaaggcgc
     5521 cgcgcaaggg atgtgatggg ctgggtaaaa ctacctagga agcagggcct acgggatgaa
     5581 gacggtctcc ccggcctgct cctcggcgtg gtagctgctt cgcacgagcg gacccgacgc
     5641 gacctggcgg atgcctagcc cgagtccgta ctcgcggtag tgcgcgaact cgtcgggcgt
     5701 gacgtagcgc tcgagcggga gatggttctt cgacggcggc aagtactggc cgatggtggc
     5761 catcgacacg ccctgggcgt ggatgtcgcg caggagctct tccacttcct ccaccgtctc
     5821 gccgatcccg accatgatcc ctgacttcgc ggggatcgcg ggctcccacg acgcgacgcg
     5881 cttcaggagc tcgagcgagc ggtcgtagcg ccccgcgggg cggacgcgct tgtagaggcg
     5941 cttcacggtc tcgaggttgt ggttcaacac ctcggggcgc gcctcgagga cggtccggag
     6001 cgcggcctcg tcgccgagga agtcgggaat gagcacctcg accttcgcgc tcgggatcgc
     6061 ctcgtggatg gcgcggatcg tctgcgcgaa gtgctccgcg cctccgtccg gcagctcgtc
     6121 ccggttcacc gaggtgacga ccgtgtagtg gagaccgaga cgccgcaccg cgtcggccac
     6181 gcgggcgggc tcgtcgggat ccaccgggag cggcttcccg ctccggatgt cgcagaacgg
     6241 gcacgcgcgc gtgcagacgt ttccgaggat caggaacgtg gcggtgtggc gcgagaagca
     6301 gtcgccgatg ttcgggcagt tcgcgctctc gcagacggtg tggagatcca gggtgcgaag
     6361 gatcgccttc gtctcctggt actccttccc cgacgggatg cgcgcgcgga gccagtcggg
     6421 acggcgcggg cccggcggcg tcagcgtgag cggctggtac tggcgcagcg gcttccgctt
     6481 ctcgcccgcc tggctccggc ggtcgctgtc catgcgctta gcggccgcgt cgtcggcgct
     6541 agatgtggat ggtgcgcccg tcggttccaa gcattgcctc cgctgttgcc tcggccaggg
     6601 tcggatgcgc gtgcaccacg tcggccagct cgagcgccga cgcctcgaag tgccgcgcga
     6661 ccaccgactc cgcgatctgc tcggtcgcga gcacgccgat gatgtggacg ccgaggatct
     6721 cgcccgtctt cgcgtccgcg accgacttca cgaagccgtc cccttcccca aggatgctcg
     6781 ccttcgcgtt cgccgtgaag gggaacttgc ctgtcttgat ctcgcgcccg gtggaccgcg
     6841 cctgggcctc ggtgagcccg acgctcgcga cctgcggatg gcagtaggtg cagttgggga
     6901 tggccgcgta gttgaccggg tgggccttct tccccgcgat ccgctcgacc gccacgacgc
     6961 cctcgtgcga ggcgacgtgc gcgagcgccg cggtcggcac gatgtcgccg atcgcgtaga
     7021 cgccggccac gttcgtctcc atgttctcgt tcacgagcac gtagccccgg tccatcttca
     7081 cgcccacggt ctcgagcccg atgccgtcgg tgacgggctt ccgccccgcc gccatgagca
     7141 cgcgctccgt ccggacgcgc ttcgtctcgc cgccgacgac gaattcgctc cacgcggcgt
     7201 ctccctcggc gcgaacgctc ttcagatccg cgccgacgtg gatctccatc ccctgccgcg
     7261 tgaaggagcg gaggagcgcc tgcgagacat cctcgtcctc gatgggcacg agccgcggca
     7321 ttcgctcgat caggacgatc ttcgagccga aggcgtggta gatggaagcg aactccacgc
     7381 ccaccgcgcc cgctccgatc acgaggagcg actccggcac gcgatcgagc gaaaggatgt
     7441 cgtcgctcga gatgacgacc cggccgtcga caggcgccat cgggagcgag ccgaccttgg
     7501 acccggtcgc gagcacgatc gcaccggcct cgatcgtttg cacgctgccg tccgcgccct
     7561 tgacctcgat cgacttggcg ctccggagct ttcccgtgcc cttcagggtc gtgaccttgt
     7621 gcttccggag gagcccttcc gtccccatga aattcttctt caccacgcgg tccttccgct
     7681 ccatcgcggc tttcagatcg agcgtggcct cggcgacctt gatgccgaac tcgcccgccc
     7741 gcttgcaatg gagcaggacc tcggcggcct cgaggagagc cttggtggga atgcacccca
     7801 cgtggaggca cgtgcctccc accttgtcct tctcgatcac gacggtcttc aatccgagct
     7861 gccccgcgcg gatggcggcc acgtagccac ccggcccgcc tcccaggatc gcgacgtcgt
     7921 agcggtccgg gctcacggac gcggctccac ggggaaggtc ggagcgatga gccgggcgag
     7981 atcggtgagg atcgtgaggt ccgtctgcgt gaagagagcc ggcgtctcgg agtcgatgtc
     8041 gatgacgccg agcacgcggc cgtcccgctc gatcggaacc acgatctcgg agcggatgga
     8101 agcgctgcac atgaggtagc gcggatcggc gttcacgtcc ggaatgattt gcggaacgcc
     8161 gtgcgccgcc acccacccgc agatgccctg cgtgtccccg ccctcggccg tgccgattcg
     8221 gatcttgtgg tgcggcgagt cggggccgtg aaacggcccc atctcgagca tcccgtcccg
     8281 cgcgaggtag atgcccgtcc acgcgtatgc cggaaacgct tcgtgcacag cgcggacgag
     8341 atgggccagc acctctgcat gcggctcccc gcgcccgatc atggccgcga cgttgggcgc
     8401 gagcatcctc tgggcctcgg tcggaagcgg ccccggcatg gccgtggcca agtccaggaa
     8461 cggagcggag gcggtaacgg ggttcacgaa gtcctcccgg gcagagccgt cgggggcggg
     8521 ggcgggatcg cggccatcgc ccggtccttc gcacgaatca agggctcgaa gagatcgatc
     8581 ggaatcggaa agagcgtggt cgagttgttc tcggtcgcga tctcggagag cgtctggagg
     8641 tagcggagcg tgatcgcgag cggctgggtc gcgatgacgg ccgcggcctc ggtgagcttc
     8701 tggctcgcct ggagctcgcc ctcggcattg atgatcttgg cgcgtcgctc ccgctcggcc
     8761 tcggcctggc gcgcgatcgc gcgctgcatc tcctgcggca ggtcgacgtt cttcacctcg
     8821 acggtgctca ccttcacccc ccagggctcg gtgtggccgt cgatgacccg ctggagctcc
     8881 ttgttgatct tttcccgctt cgagaggagc tcatccagat ccgactcgcc gagcacgctc
     8941 cggagcgtgg tctgggccat ctggctggtc gcgtagagga agttctcgac ctcgaccacc
     9001 gcgcgcacgg ggtccatcac gcggaagtac acgaccgcgt tgaccttcac gctgacgttg
     9061 tcgcgcgtga tcacgtcctg cggcggcacg tccatcgcca cggtccggag ggagatccgg
     9121 accatcttgt cgatgatcgg gatgaggagg atgagcccgg ggcctttcat gccgatgctc
     9181 cgcccgaggc ggaagatgac ggcacgctcg tactcgcgga gcaccttgat ggcgctcgag
     9241 aggatcagga tcccgaggac gacgagcgtg attgcggtgt acgagaacat ccgtgccccc
     9301 cttacgcctt cttcacccga agcacgagcc cgtcgacacg atgcaccacg acgcgggcgc
     9361 ccttggcgat cggctggtcg gcctcggccg cccagtactc gccgtgcacg aacacctgtc
     9421 cgctcggacc gagatcggtc agcgcggttc cctgctcccc caccatgccg tgcgtacccg
     9481 tggtcggctt caagcgctgg gcgcggagcc ccgcgccgat cacgagcgcg aagaaggcga
     9541 cggtcgcgcc gacggccgtg aggatgacgg agagcgagat gcgggcgacg cccttgtcgc
     9601 ctcccaggag gatgagcgag cccaggagga gcgcggccgc gccgcccaca gtgagaatgc
     9661 cgtggctctg caccttcacg tccgccgcga aaagcaccat agcgaagacg atcaaggcga
     9721 cgccggtcaa gttgaccgga agcgcctgga acgcaaggaa cgcgagcagg aggcagatcc
     9781 ctccgaccac gccgggcaga accgcccctg gattcgacag ctcgaagagg agcccgtaga
     9841 agccgaggat gaacaggatg taggcgatgt tgggatcgat gatccgcccc aggaacttct
     9901 ggcgccagga cgcctcgtac gcgtgcgtct cggcgttcgc ggtgtggagc gtcctcgtag
     9961 tgcccgccac ggagacggtg cgtccatcga tggccttgag gagctcggtg aggtcgcgcg
    10021 cgacgaggtc gatcacgtgt tcgtggagcg cgtcctcctc gttgagcgag ctcccctcgc
    10081 gcacggcctt ctccgcccag tcggcgttcc gcccgcgctg cctcgcgagc gagcggatgt
    10141 agctcaccgc gtcgttcttc accttgttcg ccatgtcctt cgccacgtcc tgcccgccca
    10201 cgttgaccgg cgtggccgca ccgatcgacg tgcccggaga catcgcgagg acgtgcgacg
    10261 ccatcccgat gaacgtgccc gcgctcgcgg cgcgtcctcc ggagggggcg acatacacga
    10321 cgatcggcac ctcggagatc aggatccgct tgatgatgtc gcgcatggac gtgtcgaggc
    10381 cgcccggcgt gtcgagacgg atcacgagcg cctcgcggtg cgccttctcc gcgcggtcga
    10441 tcgcctggat gacggtctcc gccatgatcg gctggaccgg tccctcccag gtgaggacgt
    10501 cgacctgggg gcgatcggag ccggcgcgcg tcgagtccgc cgggtccttg gcggacgcgg
    10561 acacggccgc gatgatcgcg atcagaagcg cgagggagag aatcccccgc gcggcgaggc
    10621 gtttcacccc tttgcgcctc ggttcgagcc ggcgctggat gtctggatgc ggttgttctc
    10681 gtcgacgtgc acgacgatgg gggtgtggcg gaggagctct tcgcgctcca tctgcgcgta
    10741 ggtgaggatg atcacgagat cccccacgtg cgcgaggcgc gcggccgggc cgttcaggca
    10801 aatcacgccg ctcccgcgcg gaccgcggat ggcgtaggtg tcgaagcgct cgccgttgtt
    10861 cacgttgagc acgttcacct gctcccacgg cagg
//
LOCUS       DASTKC010000066         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_994598, whole genome shotgun sequence.
ACCESSION   DASTKC010000066 DASTKC010000000
VERSION     DASTKC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994598"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFR25_02960"
     CDS             <1..306
                     /locus_tag="VFR25_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9940039.1"
                     /translation="RELLAQVGLADKADRAAGRLSGGEMQRVAIARALAHDPKLVLAD
                     EPTGNLDQANAEAVLSHLASLARESGVALVLATHSEAAAAIADRRVHLVDGRIAEIH"
     gene            311..>2493
                     /locus_tag="VFR25_02965"
     CDS             311..>2493
                     /locus_tag="VFR25_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9940040.1"
                     /translation="MALGQLLAALGLRPLGRHYGRTLLTLLGVAAGVGVYVGIDLAVQ
                     ASVQSFTRTARAVAGPAQLRIHRLPLALPESVLVALKPIEGSALFRPTLEAPARAGGP
                     TGYPFTILGVDLVGDPRFLEAEREGNRRSPRSSGLIAEDPRADVKALFDRRSLWVPLT
                     LAHAIGARDSITVVVGTRAHRFQIHPIRVSGPITSAAATVAFMDVAPFQEEFGRVGEL
                     DWIDVEPAPGVAVEPLREEIQRFLEGSRTWADLRVEPPEERSLQVERMLGSYRRNLRA
                     LSLISLVVGMLLAYNALLSSVLQRRSEIALLRALGASRGLVAILLGAEGIVVGLLGGA
                     LGVLLGRALSEGALRLVSRTVSDLYARSAPGVVDLTGSHWLTGIALGAASAAVASIGP
                     LREGLRVRPLEFLREEHPGRAERSWSYRRVATVLGALALAFYLIERPDGIGGSYRGYV
                     GAAAALLAGGLLALPAFVGLVRVFRAPLERLWSPAGRLAYATALAARRRLGVSVAALL
                     LAFSIVWGMASLVESFRVTVEGWADSTLRADLWITPRSRAGSPAEGSMPPSWTKTLAG
                     LPEVEDVDAFRLRELSLGGEMIWLGAGNSDVLARHGYLPLVGGGDPRPLLARAHLGRL
                     ALVSEPLARRHGIHAGSTLLIPTPTGSHVYQVMAVYRDYSSDRGYVILDRSIYLQDFR
                     DSLVTTYALYLAPGVSREAGARAAEAALGQDEVIRVTDTRSIREEV"
BASE COUNT          288 a          926 c          885 g          394 t
ORIGIN      
        1 cgcgagctcc tcgcgcaggt cggactcgcc gacaaggcgg accgcgcggc ggggcggctc
       61 tccggaggcg agatgcagcg ggtcgccatc gcgcgggcgc tcgcgcacga tccgaagctc
      121 gtcctggccg acgagccgac cgggaatctg gatcaggcga acgcggaggc ggtgctctcc
      181 cacctcgcct cgctcgcacg cgagagcggc gtcgcgctcg tcctcgcgac gcacagcgag
      241 gccgccgccg cgatcgcgga ccggcgcgtc cacctggtcg acggccgcat cgccgagatc
      301 cactagcgtg gtggccttgg gccagctgct cgccgcgctc ggcctccgtc cgctcggccg
      361 ccactacggc cgcacgctcc tcacgctcct cggcgtggcc gcgggcgtcg gcgtgtacgt
      421 cgggatcgat ctcgcggttc aggcgtcggt tcagtcgttc acgcgcaccg cccgcgcggt
      481 cgccggtccc gcgcagctcc gcatccaccg gcttcccttg gcactgcccg agtcggtgct
      541 cgtcgcgctc aagccgatcg aggggagcgc tcttttccgc cccacgctgg aggcgcccgc
      601 gcgcgcgggg ggacccaccg gatatccctt cacgatcctc ggcgtggatc tggtcggcga
      661 cccgcgcttc ctcgaagcgg agcgcgaggg gaaccgccgg tcgccgcgct cgagcggtct
      721 catcgcggag gacccgcgcg ccgacgtgaa ggcgctcttc gaccggcgct ccctctgggt
      781 gccgctcacc ctcgcgcacg cgatcggcgc gcgcgattcc atcaccgtcg tggtcggcac
      841 gcgcgcgcat cgcttccaga tccatccgat tcgggtcagc gggccgatca cgtcggcggc
      901 ggccacggtc gcgttcatgg acgtcgcgcc gttccaggag gagttcggcc gcgtgggcga
      961 gctcgactgg atcgacgtcg agccggcgcc gggggtcgcg gtggaacccc tgcgggagga
     1021 gatccagcgc ttcctggagg ggtcccgaac gtgggccgac ctgagggtcg agccgcccga
     1081 agagcgatcg cttcaagtag agcgcatgct cggctcctac cgccggaatc tccgcgcgct
     1141 ctccttgatc tcgctcgtgg tcgggatgct cctcgcgtac aacgcgctcc tctcgtcggt
     1201 gctccagcgg cggagcgaga tcgcactcct ccgcgcgctc ggcgcgtcgc gcgggctcgt
     1261 ggcgatcctc ttgggggcgg aaggcatcgt ggtcggactc ttgggcggcg cgctcggcgt
     1321 gctcctcggg cgcgcgctct ccgaaggcgc gctccggctc gtgagccgca cggtcagcga
     1381 tctctacgcg cggagcgcgc cgggcgtggt ggatctgacg ggaagccact ggctgaccgg
     1441 catcgcgctc ggcgccgcgt cggcggccgt ggcttcgatc ggtccgttac gcgaaggcct
     1501 ccgtgtgcgc ccgctcgagt tcctccgcga ggagcatccg ggtcgcgccg aacgttcctg
     1561 gtcgtaccgc cgcgtcgcga ccgtgctcgg agcactcgcg ctcgcgttct acctgatcga
     1621 gcgccccgat ggaatcggag gcagctaccg cggctacgtg ggcgccgcgg ccgcgctcct
     1681 cgcggggggg ctcctcgcgc tccccgcgtt cgtggggctc gtgcgcgtgt ttcgagcccc
     1741 gctcgagcgc ctctggtccc ctgcgggacg cctggcctac gcgacggcgc tcgcggcgcg
     1801 gcgaaggctc ggcgtgtcgg tcgcggcgct cctcctcgcg ttcagcatcg tctgggggat
     1861 ggcgtcgctc gtggagtcct tccgcgtgac cgtcgaaggc tgggccgact ccacgctccg
     1921 cgcggatctc tggatcacac cgcggagccg cgcggggagt ccggccgaag gctccatgcc
     1981 gccgtcctgg acaaagacgc tggccgggct ccccgaagtc gaggacgtgg acgcattccg
     2041 actgcgcgag ctctcgctcg ggggcgagat gatctggctc ggcgccggca actcggacgt
     2101 gctcgcacgg cacggctacc tgccgctcgt cggagggggc gacccgaggc cgctcctcgc
     2161 gcgcgcccac ttgggacggc tcgccctcgt gtccgagccg ctcgcgcgcc gccatggaat
     2221 ccacgcgggg tcgacgctcc tcatccccac gccgaccggc tcgcacgtgt accaggtgat
     2281 ggccgtctac cgcgactact cgtccgatcg cggctacgtg atcctcgatc ggtccatcta
     2341 cctccaggac ttccgcgatt cgctcgtgac cacgtacgcg ctctatctcg ctcccggcgt
     2401 ctcgcgcgag gccggcgcgc gcgccgcgga agccgcgctg ggacaggatg aggtgattcg
     2461 cgtcaccgac acccgctcga tccgggagga ggt
//
LOCUS       DASTKC010000067         6963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1941326, whole genome shotgun sequence.
ACCESSION   DASTKC010000067 DASTKC010000000
VERSION     DASTKC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941326"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFR25_02970"
     CDS             <1..425
                     /locus_tag="VFR25_02970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9940041.1"
                     /translation="LPLYNQDLEGTTPAWVEFRDRIRAADAVLFVSPEYNRSIPGGLK
                     NAIDVGSRPSGQSAFKGKPAAVISVSPGAIGGFGANQHIRQTLVFLDMPTLQQPEAYV
                     GGAASHFDAEGNLAKEDTKVFLRKFLDAFADWIARLRR"
     gene            complement(436..1152)
                     /locus_tag="VFR25_02975"
     CDS             complement(436..1152)
                     /locus_tag="VFR25_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET9940042.1"
                     /translation="MPSQPRVFVLTGAGISAESGIPTFRGVDGHWRGHAIESVATPEA
                     FARDPLLVWSFYSERREGARGKEPNAAHLALARLEERIQDRFFLCTQNVDPLHERAGS
                     RRVAHMHGRLFETRCSDPECARHPYPDEGTYTRREDLPRCTVCGALARPHICWFGEMP
                     FELETIFAALEHCDLCLVVGTSGVVQPAAGFASAAAARGAVTIYVGPEDPGNAEVFAS
                     IVRGPAAVALPPLLEAIAER"
     gene            1191..4133
                     /locus_tag="VFR25_02980"
     CDS             1191..4133
                     /locus_tag="VFR25_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9940043.1"
                     /translation="MGYHRSLMFVSRAAGSRFGPYELIDRLGQGGMGEVYRARDTRLG
                     REVALKVLPESATASSESKARFEREARAISRLNHPHICTLYDIGSEQGTDYLVMELIP
                     GDTLATRMRGEAVPEGEVVRLGAQIAEALDAAHKAGVIHRDLKPGNLIVTPGGNIKVV
                     DFGLAKAESDHAPPADTTQLETSPGVILGTVAYMSPEHARGGGIDERSDVFSLGIVLY
                     QLATGRLPFTGTTALETLLAIAAAEWPRVEQGALPAGLERVLRRCLERDPVQRYSSMA
                     ELKADLTRLADPGARDRPHHLPPSATSFIGREIEIAELSELIGASRLVTITGAGGSGK
                     TRLAMETARRLAPRFDAGVRQVSFDPELDPSLVASAIAEAFGVVDQEESTVLDRLAAE
                     IGARELLLVVDGCEHVLDETARALESLLGAAPKLRVLATSRESLNVAGEHVWPAPPLS
                     LPESAVGVEAAMSSDAVRLFHDRAVARDPRFALTGGNIEAVLSICRRLEGIPLAIELA
                     VARIPAMAPAEIERRLDDCFRVLSGGRRGGPARHQTLRAALDWSHDLLSEPERVLYRR
                     LAVFAGGFDLEQAESVCGFGNLPEGDVLDTLARLVDKSLVVSRHDDEGRTRYRLLEPL
                     RQHAEEHLRAAGELDAIARRHAERMAAFADRAYDERIRDPARWVERLERDHDNLRAAL
                     AWTAAHDAEVDLRLAGALAWFWQMHGHYREGRQALRRVLGRERRRTRETARALAGASV
                     LAAWGGEAGGGGSAAEESLAIWRELGDAREVAAALEGIGWSRWIAEDDAGAIAAFEEG
                     LATFERLGDAPMVNRAKLAIAQVLVGIPDVGRALAIVRDVLPSAIEQRNARDIHFALH
                     FQADCALIAGDARSAEVFYTDSLRAAIDAGDPSEMCFEIEGIAMALAGQGRDRKAMVL
                     VGALKAERERLGVDFHVRFWDALIDRFLTPARERMGSGAAAAEREGRAMGFRTAIDYA
                     LSSDRDAS"
     gene            4215..5207
                     /locus_tag="VFR25_02985"
     CDS             4215..5207
                     /locus_tag="VFR25_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET9940044.1"
                     /translation="MKRILLVLFACLVPALARAQTIDLTTVGATFFGADGARWYQGTT
                     FAGTTSFVGFLRVQATPTERGFNTDFRPLVEDDIGTLTFTHSITFGVFQPRILTDATG
                     AYRFYTLLLDVNESSTNGDNYISLDSLTVYSVPAGSGGSLSTRAAIAAAGTVRYDMDQ
                     PANQVVLIDGNLVVGSGLNDIEIDIPASRFDGVGASDFIYVLVHFGQLGTVSTRKYGT
                     SGGFEELRYSQTVTDVEEASSAATGPWIRMMGAPSGALRAHYYVPGAGQVRLTLYDIH
                     GRAVASTSFREDAGGMRDLPLHTAPSVSRLASGIYFYRFEWNDTRRSGKVAILR"
     gene            5298..6932
                     /locus_tag="VFR25_02990"
     CDS             5298..6932
                     /locus_tag="VFR25_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9940045.1"
                     /translation="MTRFVGLLAASICIALSPVGCGSQPAPREVEGKLRTQVPAFVGK
                     PGATVRGEPLLEREAVAKFYEGRQYRSAWPSERSTNDLLDAIKDVDADGLKPSDYHLD
                     AIQKLVDEQKTAHDPAIQADLDILMTDAVAAIVDDMRYGRVRPASVNPAWNMDPREGA
                     PPLETELGKVVGADRVRTALQQERPQHFIYQGLVKALADLRKIESSGGWRTIPPGPTL
                     KAGMRNARVAALRDRLQASGELEAGRSADSLRFDADVDRAVKLFQARHRLDEDGVVGK
                     GVLAELNVPVASRIGQVRVNLERARWVLGGLQSDFVLVNLPAFKAYLIRGGRNIWESR
                     IVIGQEARQTPSFRATMQTVVFNPDWTVPPTILAEDVLDGMRQGKNVLAEKRLQVYDK
                     DGNEVNPSSIDWDSASPDNFPYTLKQDPGEDNALGRVKFLFPNKYSIYLHDTPSKSLF
                     ESNKRTFSSGCIRIERPLDLAEILLSGQDGWNRAKMEDVIAQGTTTNVQLEHGLPVVI
                     VYWTVSVGASGEVRYTRDPYDLDPPLLQALNGARRS"
BASE COUNT         1110 a         2384 c         2397 g         1072 t
ORIGIN      
        1 ggctgccgct ctacaaccag gacctcgaag gtacgacacc agcctgggtg gaattccggg
       61 accggatccg agccgccgat gccgtcctct tcgtctctcc cgagtacaac cgctccattc
      121 ccggcggtct caagaacgcc atcgatgttg ggtcgcgtcc ctccggacag agcgcgttca
      181 aggggaagcc ggcggccgtg atcagcgtgt ctccgggtgc gatcggcggg tttggggcga
      241 accagcacat ccgccagacc ctggtctttc tcgacatgcc gacgctgcag cagcccgaag
      301 cctacgtggg cggggcagct tcacacttcg atgcggaggg gaatctcgcc aaggaggaca
      361 caaaggtgtt cctccggaag ttcctggacg ctttcgcgga ctggatcgcg cgcctgcgcc
      421 ggtagctggg tgtgctcagc gctctgcgat cgcctcgagg agaggcggga gcgccaccgc
      481 tgcgggaccc cgaacgatcg acgcgaacac ctcggcgttc cccggatcct ccggccccac
      541 gtagatcgtg accgcgccgc gcgcggctgc cgcggaggcg aagcccgccg ccggctgcac
      601 gacacctgac gtccccacca cgagacagag atcgcagtgc tcgagcgcgg cgaagatcgt
      661 ctccagctcg aacggcattt caccgaacca gcagatgtgc ggccgcgcga gggcgccgca
      721 gacggtgcag cggggaaggt cctcgcggcg cgtataggtc ccctcgtccg ggtagggatg
      781 ccgcgcgcat tcgggatcgg agcagcgtgt ctcgaagagc cggccgtgca tgtgcgccac
      841 gcgcctcgac cccgcacgct cgtggagcgg gtcgacattc tgcgtgcaga ggaagaagcg
      901 gtcctggatc cgctcttcga gccgcgcgag cgccaggtgc gccgcgtttg gctccttgcc
      961 gcgcgctccc tcccgccgct cggagtagaa gctccagacc aggaggggat cgcgcgcgaa
     1021 ggcttccggc gtggccaccg attcgatcgc atgcccgcgc cagtgcccgt ccacgccgcg
     1081 gaacgtgggg atgccgctct ccgcgctgat gccggcgccg gtcaggacga agacgcgcgg
     1141 ctgggatggc aaggaatcaa gggccgggtc catgtgacgg agagtaacgc atgggctatc
     1201 atcggtcgct catgttcgtc tcccgcgccg caggaagccg cttcggtccg tacgagctga
     1261 tcgaccgcct gggccagggc gggatgggcg aggtctatcg cgcccgcgac acgcgtctcg
     1321 ggcgcgaggt cgcgctcaag gtgcttcccg aaagcgccac ggcttcgtcc gagtccaagg
     1381 cgcgcttcga gcgcgaggcc cgcgccatct cccggctcaa ccacccccac atctgcaccc
     1441 tctacgacat cgggagcgag caggggaccg actacctcgt catggagctc atccccggcg
     1501 acaccctcgc gacccgcatg cggggcgagg cggttccgga gggcgaggtc gttcgcctcg
     1561 gggcgcagat cgcggaggcg ctcgacgccg cgcacaaggc cggggtgatc catcgcgatc
     1621 tgaagccggg gaatctcatc gtgacgccgg gcgggaacat caaggtcgtg gacttcggtc
     1681 tcgccaaggc cgagtcggat cacgcgccac ccgccgacac gacgcagctc gagacctcgc
     1741 cgggcgtgat cctgggcacc gtcgcctaca tgagcccgga acacgctcgg ggaggcggga
     1801 tcgacgagcg gtccgacgtg ttcagcctcg gcatcgtgct ctaccagctc gcgaccggac
     1861 ggcttccgtt cacgggaacg acggcgctcg agacactcct cgccattgct gccgcggaat
     1921 ggccgcgggt cgaacagggc gcgctcccgg ccggcctcga gcgggtgctc cgccggtgcc
     1981 tcgagcgcga cccggtccag cgctattcgt ccatggcgga gctcaaggcc gatctgaccc
     2041 ggctcgcaga tcccggcgcg cgcgaccgtc cgcaccatct gccaccgtcc gcgacctcgt
     2101 tcatcgggcg cgagatcgag atcgccgagc tctcggagct catcggcgcg agccggctcg
     2161 tgacgatcac gggggctgga ggctccggga agacgcgact cgcgatggag accgcgcgcc
     2221 gccttgctcc gcgcttcgac gcgggtgtcc gccaggttag cttcgacccc gagctcgatc
     2281 cgtcgctcgt tgcctcggcg atcgccgagg cgttcggcgt cgtcgaccag gaggaatcga
     2341 cggtgctcga ccggctcgcc gccgagatcg gagcgaggga gctcctcctc gtcgtggacg
     2401 ggtgcgagca cgtgctcgac gagacggcgc gcgccctcga atccctcctg ggagcggccc
     2461 caaagcttcg cgtcctcgcc accagccgcg agagtttgaa cgtggcgggg gagcatgtct
     2521 ggccggcgcc ccccctgtcc ctgccggaga gcgcggtcgg cgtggaggcc gcgatgagct
     2581 ccgacgccgt gcggctcttc cacgaccggg cggtcgcgcg cgatccccgc ttcgcgctca
     2641 cgggcggaaa catcgaggcg gtcctgtcga tctgccgccg tctcgaaggc atccccctcg
     2701 ccatcgagct ggcggtggcg cgcattccag ccatggcccc cgccgagatc gagcgccgcc
     2761 tcgacgattg cttccgcgtc ttgagcggcg ggcgtcgcgg cggtccggcg cgccaccaga
     2821 cgcttcgcgc ggcgctcgac tggagccacg acctcctgtc cgagccggag cgcgtcctct
     2881 atcgacggct ggccgtcttc gcggggggct tcgatctcga gcaggcggaa tccgtctgcg
     2941 gcttcggcaa cctcccggaa ggcgatgtgc tcgacaccct cgcgcgtctc gtcgacaaat
     3001 ctctcgtcgt cagccggcac gacgacgagg gccgcacgcg ctaccgcctg ctcgagccct
     3061 tgcggcagca cgccgaagag cacctccgcg ccgccggcga gctcgacgcg atcgcgcggc
     3121 ggcatgccga gcgcatggcc gcgttcgcgg accgcgcata cgacgagcgg atccgagatc
     3181 cggcgcgctg ggtcgagcgc ctggaacgcg accacgacaa cctgcgcgcg gcgctcgcct
     3241 ggacggcggc gcacgatgcc gaggtcgatc tgaggctggc cggagcgctc gcgtggttct
     3301 ggcagatgca cggccactat cgcgagggac gtcaggcgct ccgccgcgtg ctggggcgcg
     3361 agcggcgacg aacgcgcgag acggcgcgcg cgctcgcggg ggctagcgtc ctcgcggcct
     3421 ggggcggtga agcgggcggc ggcgggagcg cggcggagga gagcctcgcg atctggcgcg
     3481 agctcgggga tgcgcgcgag gtcgccgcgg cgctcgaagg gatcggctgg agccgatgga
     3541 tcgcggagga cgatgccgga gcgatcgcgg cgttcgagga aggtctcgcg acgttcgagc
     3601 ggcttggaga cgcgccgatg gtgaaccgcg cgaagctggc gatcgcgcag gtgctcgtgg
     3661 gcattcccga tgtcgggcga gctctggcga tcgtgcgcga tgtgctcccg tccgcgatcg
     3721 agcagcgaaa cgcgcgcgat atccacttcg cgctccactt ccaggcggac tgcgcgctca
     3781 tcgcgggaga tgcccgaagc gccgaagtgt tctacaccga cagtctgcgc gcggccatcg
     3841 acgccggcga tccctcggag atgtgcttcg agatcgaagg gatcgccatg gcgctcgcgg
     3901 ggcagggacg cgaccggaag gcgatggttc tcgtcggagc gctcaaggcg gagcgggaac
     3961 ggctcggcgt cgatttccac gtgcgcttct gggatgccct catcgatcgg ttcctcacgc
     4021 ccgcccgcga gcgcatgggc tctggggccg cggccgcgga gcgggaggga cgcgcgatgg
     4081 gattccgaac cgccatcgac tacgccctat cctccgaccg cgacgcgagc tgagctatac
     4141 tcagaggacc cggccgggct accaatgccc cattcgagcc gggcgctcac gcatcggtga
     4201 ccggggggac acctatgaag cgcatcctgc tcgtcctgtt cgcctgcctc gtgccagccc
     4261 tcgcacgcgc gcaaaccatc gacctcacga cggtaggagc gaccttcttc ggtgcggacg
     4321 gagcccgctg gtatcagggc acgacgttcg cggggaccac atcgttcgtg ggtttcctgc
     4381 gcgtgcaggc gacccccacc gagcggggct tcaacaccga ttttcgcccc ttggtggaag
     4441 acgacatcgg gacgctcacg ttcacgcatt cgatcacatt cggcgtgttc cagccccgaa
     4501 ttctgacaga tgcgaccggc gcgtatcggt tctacaccct gcttctcgac gtgaacgagt
     4561 cctcgaccaa tggagacaac tacatctccc tcgattccct gaccgtctac tcggtgccgg
     4621 cggggagcgg tggatcgctg agcacccgcg ccgcgatcgc ggccgcgggc accgtgcgat
     4681 atgacatgga ccagcctgcc aaccaggtcg tgctgatcga tggaaatctg gtcgtgggca
     4741 gcggcctgaa cgacatcgag atcgacattc ccgcctcccg attcgacggc gtcggcgcca
     4801 gcgacttcat ctacgtcctg gtgcatttcg ggcagctcgg aacggtttcg acgcggaagt
     4861 acggaacatc gggcggcttc gaggagctgc gctactcgca gactgtgacc gacgtggagg
     4921 aggcttcctc ggcggcaacg ggaccgtgga ttcgcatgat gggcgcgccg tcgggcgcgc
     4981 ttcgcgccca ctactacgtc ccgggggcgg ggcaggtgcg tctcaccctg tacgacatcc
     5041 acggtcgtgc cgtcgcatcc acctccttcc gggaggacgc cggcgggatg cgagacctgc
     5101 cccttcacac cgcgccgagc gtctctcgcc tcgcctcggg tatttatttc taccgattcg
     5161 aatggaacga tacgcggcgg agcgggaaag tcgcgatcct gcgttgagtt aggtcggagc
     5221 cctctgcggt ccgtatcttg ctgcacgttc cccgatgagg cccgttgggc ccatttcata
     5281 catcggggga tccagccatg actcgcttcg tcggcctgct cgccgcatca atttgcatcg
     5341 ccctctcgcc ggtcggctgc ggctcgcagc cggctccgcg tgaagtcgag ggcaagctac
     5401 gcactcaggt ccccgcgttc gtcggcaagc ccggcgcgac cgtccgcggc gagccgctgc
     5461 tcgagcgcga agccgtcgcc aagttctacg aaggacgcca gtaccggtcc gcctggccat
     5521 cggagcggag caccaacgat ctcctggacg cgatcaagga cgtcgatgcg gacggcctca
     5581 agccctcgga ctaccacctc gacgcgatcc agaagctcgt cgacgagcag aagacggcgc
     5641 acgatcccgc catccaggcc gatctcgaca tcctcatgac cgacgccgtg gcggcgatcg
     5701 tcgacgacat gcgctacggg cgcgtgcgtc cggcgagcgt gaatccggcc tggaacatgg
     5761 atccgcgcga gggtgcgccc ccgctcgaga cggagctcgg caaggtggtc ggagccgatc
     5821 gcgtgcggac ggcgctccag caggagcggc cgcagcactt catctaccaa ggactcgtga
     5881 aagcgctcgc cgatctgcgg aagatcgaat ccagcggcgg gtggagaacc atcccccccg
     5941 gtccgacgct caaggcgggc atgcgcaacg ctcgcgttgc cgcgctccgg gaccgcctcc
     6001 aggcgtccgg cgagctcgag gcgggacgga gcgccgactc cctccggttc gacgccgacg
     6061 tggatcgcgc ggtgaagcta ttccaggcgc gccaccggct cgatgaagac ggcgtggtgg
     6121 ggaagggcgt gctggccgag ctcaatgtgc cggtcgcctc gcgcatcggt caggttcgcg
     6181 tgaatctcga gcgcgcgagg tgggtgctcg gaggcctcca gagcgacttc gtgctggtga
     6241 acctgcctgc gttcaaggcg tacttgatcc ggggcggccg gaacatctgg gagtcgcgca
     6301 tcgtgatcgg ccaggaggcg cgacagacgc cgagcttccg cgccaccatg cagaccgtcg
     6361 tcttcaatcc cgactggacg gtgcccccga cgattctcgc cgaggacgtg ctcgacggca
     6421 tgcgccaggg gaagaacgtg ctcgccgaga agcggctcca ggtctacgac aaggacggaa
     6481 acgaggtgaa tcccagctcc atcgactggg actccgcctc gccggacaac ttcccgtaca
     6541 cgctcaagca ggatccggga gaggacaacg cgctcggccg ggtgaagttc ctcttcccga
     6601 acaagtactc gatctatctc cacgacacgc cgagcaagag cctgttcgaa tcgaacaagc
     6661 ggaccttcag ctccggctgc atccggatcg agcgccctct cgatctcgcg gagatcctgc
     6721 tctcggggca ggacggttgg aaccgcgcga agatggagga cgtcatcgcg cagggaacga
     6781 cgacgaatgt gcagctcgag cacgggcttc cggtcgtgat cgtctactgg acggtgagcg
     6841 tgggcgcgtc gggggaggtg cgttacaccc gggaccccta cgatctcgac cctccgctcc
     6901 tccaggcgtt gaacggggcg aggcggtcgt agcaacgcgc ggcggcatcg agtcgacggc
     6961 agg
//
LOCUS       DASTKC010000068        10064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_49276, whole genome shotgun sequence.
ACCESSION   DASTKC010000068 DASTKC010000000
VERSION     DASTKC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49276"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2539)
                     /gene="rpoC"
                     /locus_tag="VFR25_02995"
     CDS             complement(<1..2539)
                     /gene="rpoC"
                     /locus_tag="VFR25_02995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9940046.1"
                     /translation="MLASPETIRSWSHGEVTKPETINYRSFKPERDGLFCEKIFGPVK
                     DWECNCGKYKRIRYRGVICDRCGVEVTQAKVRRERLGHINLAVPVSHIWYFKAVPSRI
                     GHLLDMSIRDLERVLYYESYVVIDSGTSGLKKKQLISEDEYYELTQDETVTLTAKMGA
                     EAIRDLLQEIDLDELSAELRAIAKIENSVQRKKETLKRLRIVEAFRQSGNRPEWMILG
                     VIPVLPPDLRPLVPLEGGRFATSDLNDLYRRVINRNNRLKKLIDIRAPEVILRNEKRM
                     LQEAVDALFDNGRRSRAVRGQGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIV
                     VGPELKLHQCGLPKNMALELFKPFIIRKLEDKGFVQTVKSAKRLVERERPEVWDILGD
                     IINGHPVLLNRAPTLHRLGIQAFEPVLVEGKAIRIHPMVCGAFNADFDGDQMAVHVPL
                     SFEAQIETRMLMLSTNNILLPSNGRPVAAPSQDIVLGCNYMTKARPLAQGAKRKMYGS
                     MDEVRAAYDHKQAGLHDPIVCRIEGKRIETTVGRVLFNEALVPWGISFNNDEMDKKAL
                     EQLVGDCHRFLGDKTTALLLDSLKNLGFTYATQAGITVGIDDILIPARKTEIIGRAQG
                     DVASINNQYQQRVITDGERYNQVINTWTLVTTEVEEETFKGLAKDRDGFNPIFMMAHS
                     GSRGTKEQIRQLAGMRGLMAKPQKKITGGLGEIIESPVIHNFKEGLTVLEYFVSTHGA
                     RKGLADTALKTADAGYLTRRLVDVAQDVIINIEDCGTVNGLEVAALKEGEEIIEPLGD
                     RVLGRVAIEDIVDPNTSDVIVRAGEEINEVAAEAIEESGRIEKVSIRS"
     gene            complement(2678..6544)
                     /gene="rpoB"
                     /locus_tag="VFR25_03000"
     CDS             complement(2678..6544)
                     /gene="rpoB"
                     /locus_tag="VFR25_03000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9940047.1"
                     /translation="MKASRGKERRSFSKIEREWDIQIPNLLDVQLDSFRNFLQADIDP
                     LKRKLEGLQEVFQSVFPISDPREFFSLEFVRYDLGEPKYSVDECQERDLTFSVPLKAT
                     LRLRIREDTDEGRKDKSIIEQEVYLGELPIITDKGTFIINGAERVIVSQLHRSPGVFF
                     DESSHPSGKKLFTARIIPYRGSWVEFSLDVNDIMYVHIDRKRKLPVTVLLKALGYVSD
                     REILQLFYDTEEEEIGGARSKKEPDCLGKVSAEDVVDEKTGEVLLEANEVISMERIEG
                     LRKAGFSTVKAFVIAHSDEADILRNTLKKDSTKAEDEALTRIYNLLRPGEPPRADTAR
                     DILKKLFFNPKRYDLAKVGRYKLNRKLPHDVILGGKDRRKKLALEVPDDNQTTLGPED
                     FLVIIAYLMALRLGTEVPGITVSTDDIDHLGNRRVRAVGELLANQFNVGLARMARIIR
                     ERMTLQDAEQVTPYDLINARTISAVIQSFFGSSQLSQFMDQTNPLAELTHKRRLSALG
                     PGGLTRDRAGFEVRDVHYTHYGRMCPIETPEGPNIGLISSLATYARVNELGFLETPYR
                     RVDKGAVDKKQVQFLSADVEDQYKIAQANAAVDGGGHLQGLQNVRERGEFPMVEPSNV
                     DFMDISPIQLVSPAAALIPFLEHDDANRALMGSNMQRQAVPLLYTEAPLVGTGLEQKV
                     AEDSGALVLAKRPGVIEAVSGDSILVRYDQPDGRSRAVDYSEMAGIDSYRLTKFKRSN
                     QDTCINQRPLVSEGDHVKKGQLLADGAATRQGELALGANVLVAFMPWGGYNFEDAILV
                     SERLIKDDRFTSIHIEEFELQVRDTKRGAEEITREIPNVSEEAVKNLDEEGIVRIGAR
                     VKAGDILVGKVTPKGETDLTPEERLLRAIFGEKAGDVRDASLKAPPGMDGVVIDTKVF
                     SRREKDESTKKQEKRKIDRLRRQAKKEKDRILETRAAEVGKALIGEFVNKWVEGDGDK
                     SVARSGKKIDEEFLSKADLDSIPYRTPVTRDDAINDRFWSILEGAARAMDRVEKNLEK
                     EIEKVTRGDELPPGVVKLVKVYVAKKRKLSVGDKMAGRHGNKGVVAKILPEEDMPYLP
                     DGTPVDMVLNPLGVPSRMNIGQILETHLGWAAHELGNTVATPVFAGATVDEIKACLRD
                     ANLPEDGKTVLYDGRTGESFDQRVTVGFIYMMKLSHLVDDKIHARSIGPYSLVTQQPL
                     GGKAQFGGQRFGEMEVWALEAYGAAYTLQELLTVKSDDVTGRSRIYEAIVKGENPPSP
                     STPESFNVLVKELQSLCMDVQLRDEP"
     gene            complement(6597..6989)
                     /gene="rplL"
                     /locus_tag="VFR25_03005"
     CDS             complement(6597..6989)
                     /gene="rplL"
                     /locus_tag="VFR25_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET9940048.1"
                     /translation="MEAATKDQQIITLLEGMTMLEVSGLVKKLEEHFGVTAAMPMMAA
                     MPGMGGGAGAAAAVEEKTEFDVVLTGAGDKKIQVIKVVREVTGLGLKEAKDLVDGAPK
                     PVKEKVSKEEAGSIKTKLEENGASVEIR"
     gene            complement(7048..7563)
                     /gene="rplJ"
                     /locus_tag="VFR25_03010"
     CDS             complement(7048..7563)
                     /gene="rplJ"
                     /locus_tag="VFR25_03010"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET9940049.1"
                     /translation="MALVEKEQEVRELAELLAKAKSIYLTDFQGMNVELVSRMRRKLR
                     DAKVEYRVSKNTLTKRALKDHGVTSLDSYLEGPTGIAFAPDEVSAAKILADFSKEFEK
                     PALKAAYVAGHVYGPDGIKSLAALPPREVLLGQFIGGLRSPMQGVVGVLAGSLRQLVG
                     VVDAVGKKKQA"
     gene            complement(7601..8305)
                     /gene="rplA"
                     /locus_tag="VFR25_03015"
     CDS             complement(7601..8305)
                     /gene="rplA"
                     /locus_tag="VFR25_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET9940050.1"
                     /translation="MKQGKRFKKAVEVTGLDRLGDLQDAVERLKKSATAKFDETVEVS
                     MSLGVDPRHAEQQVRGTVVLPHGTGKKIRVLVLTKGEKEREAKDAGADYAGSEEFIKR
                     LNEGWLDIDSIIATPDMMGEVGKLGRVLGPRGLMPNPRSGTVTMDVAKAVREVKSGKI
                     EYRVDKAGNLHAPVGKASFGKDQLVQNVEVFLREVIRQKPAAAKGQYLRSITLSSTMG
                     PPVRLDPAAIQAGLKA"
     gene            complement(8302..8733)
                     /gene="rplK"
                     /locus_tag="VFR25_03020"
     CDS             complement(8302..8733)
                     /gene="rplK"
                     /locus_tag="VFR25_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9940051.1"
                     /translation="MAKSKPVQAIVKLQVPAGNATPAPPIGPALGQHGVNIMEFCKGF
                     NARTQGQPGLIIPVVITVYNDRSFTFITKTPPAAVLLKRAAGIAKGSGEPNRNKVAKV
                     TAAQVREIAELKKPDLNSNDLEAAIRMVEGTARSMGIEVAG"
     gene            complement(8824..9351)
                     /gene="nusG"
                     /locus_tag="VFR25_03025"
     CDS             complement(8824..9351)
                     /gene="nusG"
                     /locus_tag="VFR25_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET9940052.1"
                     /translation="MQWYVIHTYSGHENKVKANLERAIHSAGLEPNFGQILVATEEFA
                     EMKDGKRITSKRKTFPSYVLVEMDLNQDTRLLVQNVPGVTRFVGSGQEPIPLREAEVK
                     RILGHMEAGGRLRGSAEVPFKSGEHVKVVDGPFTDFTGVVDEVNPERQKVKVMVSIFG
                     RATPVELDFLQVQPV"
     gene            complement(9367..9606)
                     /gene="secE"
                     /locus_tag="VFR25_03030"
     CDS             complement(9367..9606)
                     /gene="secE"
                     /locus_tag="VFR25_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9940053.1"
                     /translation="MRAETTSEKPEVRTGPVQSLTDYVKDVRVEMSKVSWPTREELRE
                     STLVVIIMVFLMMVFIGVIDRGLSLAFEALVKMVG"
     gene            complement(9636..9713)
                     /locus_tag="VFR25_03035"
     tRNA            complement(9636..9713)
                     /locus_tag="VFR25_03035"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9676..9678),aa:Trp,seq:cca)
     gene            complement(9733..9882)
                     /gene="rpmG"
                     /locus_tag="VFR25_03040"
     CDS             complement(9733..9882)
                     /gene="rpmG"
                     /locus_tag="VFR25_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9940054.1"
                     /translation="MRDLITLACNDCKERNYTNTKNKRKHPDRVEWKKFCPRCRKHTL
                     HKETR"
     gene            complement(9887..>10064)
                     /locus_tag="VFR25_03045"
     CDS             complement(9887..>10064)
                     /locus_tag="VFR25_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9940055.1"
                     /translation="DVTGVATLPEGREMVMPGDNVEMAVELITPIAMEKELRFAIREG
                     GRTVGAGVVAEVTE"
BASE COUNT         1467 a         3451 c         3146 g         2000 t
ORIGIN      
        1 ccgagcggat cgacaccttc tcgatgcgtc cgctctcctc gatcgcctca gccgccacct
       61 cgttgatctc ttcgcccgcg cggacgatga cgtcgctcgt gttcgggtcc acgatgtcct
      121 cgatggccac gcgaccgagc acccggtccc cgagcggctc gatgatctcc tcgccctcct
      181 tgagagcggc gacctcgagg ccgttgaccg tgccgcagtc ctcgatgttg atgatcacgt
      241 cctgcgccac gtccacgagc cgccgcgtga gatagccggc gtccgccgtc ttgagcgccg
      301 tgtccgcgag acccttccgg gcgccgtgcg tggacacgaa gtactcgagc acggtgaggc
      361 cttccttgaa gttgtggatg accggcgact cgatgatctc gcccagaccg cccgtgatct
      421 tcttctgcgg cttggccatg aggccgcgca tcccggcgag ctggcggatc tgctccttcg
      481 ttccgcgcga gccggagtgg gccatcatga agatcggatt gaacccgtcg cgatccttgg
      541 cgaggccctt gaacgtctcc tcctcgacct cggtcgtcac gagcgtccag gtgttgatga
      601 cctggttgta gcgctcgccg tcggtgatca cccgctgctg gtactggttg ttgatggacg
      661 cgacatcgcc ctgcgcacgg ccgatgatct cggtcttgcg cgccgggatg aggatgtcgt
      721 cgatgccgac cgtgatgccc gcctgggtcg cgtacgtgaa cccgaggttc ttgagcgagt
      781 ccagaaggag ggccgtggtc ttgtcgccga ggaaccggtg gcagtcgccc accagctgct
      841 cgagcgcctt cttgtccatc tcgtcgttgt tgaaggagat gccccacggc acgagcgcct
      901 cgttgaagag gacccgcccg acggtggtct cgatccgctt gccctcgatg cggcagacga
      961 tcggatcgtg gaggccggcc tgcttgtggt cgtacgcagc ccggacctcg tccatgcttc
     1021 cgtacatctt cctcttcgcg ccctgtgcga gcggacgcgc cttggtcatg tagttgcacc
     1081 cgagcacgat gtcctggctc ggagcggcga ccggccggcc gttcgacggg agcaggatgt
     1141 tgttcgtcga gagcatgagc atccgcgtct cgatctgcgc ctcgaacgac agcggcacgt
     1201 gcaccgccat ctggtccccg tcgaagtcgg cgttgaacgc gccgcacacc atcgggtgga
     1261 tccggatcgc cttgccctcg accagcaccg gctcgaacgc ctggatgccg aggcggtgga
     1321 gggtcggcgc gcggttcagg agcaccgggt gtccgttgat gatgtcgccg aggatgtccc
     1381 agacctccgg gcgctcgcgc tccacgagcc gcttcgcgct cttcacggtc tggacgaatc
     1441 ccttgtcttc gagcttccgg atgatgaacg gcttgaacag ctcgagcgcc atgttcttcg
     1501 gcagcccgca ctggtggagc ttgagctcgg ggccgaccac gatgaccgag cggcccgagt
     1561 agtccacgcg cttgccgagc aggttctgac ggaaccggcc ctgcttgccc ttcaacatgt
     1621 ccgagagcga cttgagcggg cggttgccct gaccgcgcac ggcgcggctg cggcggccgt
     1681 tgtcgaacag cgcgtcgacg gcctcctgga gcatccgctt ctcgttccgg aggatgacct
     1741 caggcgcccg gatgtcgatg agcttcttca accggttgtt ccggttgatg acgcggcggt
     1801 agagatcgtt gagatccgac gtcgcgaacc ggccgccttc gagcgggacg agcgggcgga
     1861 gatccggcgg cagcaccggg atcacgccca ggatcatcca ctcgggacgg ttgccgctct
     1921 ggcggaacgc ctcgacgatc cggagccgct tcagcgtctc cttcttccgc tggaccgagt
     1981 tctcgatctt cgcgatggcg cgaagctcgg ccgagagctc gtccagatcg atctcttgca
     2041 ggaggtcgcg gatcgcctcg gcgcccatct tggccgtgag cgtgaccgtc tcgtcctgcg
     2101 tgagctcgta gtactcgtcc tcggagatga gctgcttctt cttgaggccg ctcgtcccgg
     2161 aatcgatgac gacgtacgac tcgtagtaga ggacccgctc gagatcgcgg atcgacatgt
     2221 cgaggaggtg cccgatgcgg ctcggcaccg ccttgaagta ccagatatgg gacaccggaa
     2281 ccgcgaggtt gatgtggccg aggcgctccc ggcggacctt cgcctgggtc acctccacgc
     2341 cgcaccggtc gcagatcacc ccgcggtagc ggatgcgctt gtacttgccg caattgcact
     2401 cccagtcctt caccggaccg aagatcttct cgcagaacaa cccgtcccgc tccggcttga
     2461 acgaccggta gttgatcgtc tccggcttcg tgacctcgcc gtgcgaccag ctccggatcg
     2521 tctccgggga cgcgagcatg atgcggatcg agttgaacga gagccggcgc gtgcggaggt
     2581 cccgctcgtc cggacgcgtc agcgagaagc gctggcgctc ggtcgcgagc ggagacttga
     2641 ttccgaacag ttcctgcatc gtcaatgcct cctcggctta gggctcatct cgaagctgga
     2701 cgtccatgca aaggctctgg agctccttca ccagcacgtt gaacgattcc ggcgtgctgg
     2761 gtgagggcgg gttctcgccc ttcacgatcg cctcgtagat gcgcgaccgg cccgtgacgt
     2821 cgtccgactt caccgtgaga agctcctgga gcgtgtaggc cgcgccgtaa gcttcgagcg
     2881 cccacacctc catctcgccg aaccgctggc ctccgaactg cgccttgcct cccagcggct
     2941 gctgggtgac gagcgagtac ggtccgatcg agcgggcgtg gatcttgtcg tcgaccaggt
     3001 gcgagagctt catcatgtag atgaagccca ccgtcacgcg ctgatcgaac gattcccccg
     3061 tccgcccgtc gtagaggacc gtcttcccgt cctcgggcag gttcgcgtcc cggaggcagg
     3121 ccttgatctc gtccaccgtc gcgcccgcga agaccggggt cgcgaccgtg tttccgagct
     3181 cgtgggccgc ccaaccgaga tgcgtttcca ggatctgacc gatgttcatt cgcgaaggca
     3241 cgccgagcgg gttcagcacc atgtcgaccg gcgttccgtc cgggaggtac ggcatgtcct
     3301 cctcggggag gatcttcgcg acgacgccct tgttgccgtg gcgtccggcc atcttgtcgc
     3361 caaccgagag cttcctcttc ttggcgacgt acaccttcac gagcttgacc acgccggggg
     3421 gcagctcgtc cccgcgcgtg accttctcga tctccttctc gagattcttc tcgacccggt
     3481 ccatcgcgcg cgccgcccct tcgaggatgc tccagaagcg gtcgttgatc gcgtcgtcgc
     3541 gcgtgacggg cgtgcggtac ggaatcgagt ccagatcggc cttggagagg aactcctcgt
     3601 cgatcttctt gccggagcgc gcgaccgact tgtcgccatc gccctcgacc cacttgttga
     3661 cgaactcccc gatgagggcc ttgccgacct cggccgcccg cgtctcgagg atccggtcct
     3721 tctccttctt ggcctggcgg cggagccggt cgatcttccg cttctcctgc ttcttggtcg
     3781 actcgtcctt ctcgcgccgg ctgaacacct tggtgtcgat gaccacgccg tccatgcccg
     3841 gcggggcctt gagcgaggcg tcgcgcacgt cgccggcctt ctcgccgaag atcgcgcgga
     3901 ggagccgctc ctcgggcgtc aaatccgtct cgcccttggg cgtcaccttg cccacgagga
     3961 tgtcgcccgc cttgacgcgg gcgccgatcc gcacgatccc ttcctcatcg aggttcttga
     4021 ccgcctcctc gctgacgttc gggatctcgc gggtgatctc ctcggcgccg cgcttggtgt
     4081 cgcggacctg gagctcgaac tcctcgatgt ggatggacgt gaagcggtcg tccttgatga
     4141 gccgctccga cacgaggatc gcgtcctcga agttgtagcc gccccacggc atgaacgcga
     4201 cgagcacgtt ggcgccgaga gcgagctcgc cctggcgcgt cgccgcgccg tcggcgagga
     4261 gctgcccctt cttcacgtgg tcaccctcgc tcacgagggg ccgctggttg atgcacgtgt
     4321 cctggttcga gcgcttgaac ttggtgaggc ggtagctgtc gatgcccgcc atctccgagt
     4381 agtccacggc gcgcgaccgg ccgtccggct ggtcgtagcg cacgaggatg gaatcgcccg
     4441 aaaccgcctc gataacgccc ggccgcttgg ccaggacgag cgcccccgaa tcctccgcga
     4501 ccttttgctc gagaccggtg ccgacgagcg gcgcctcggt gtagaggagc ggcactgcct
     4561 gacgctgcat gttggagccc atgagcgcgc ggttcgcgtc gtcgtgctcg aggaacggga
     4621 tcagggccgc cgcagggctc acgagctgga tcggcgagat gtccatgaag tcgacgttcg
     4681 acggctcgac catggggaac tcgccccgct cgcgcacgtt ctgcagtccc tgcaggtgtc
     4741 cgccgccgtc cacggccgcg ttcgcctgcg cgatcttgta ctggtcctcc acgtcggccg
     4801 agaggaactg gacctgcttc ttgtccacgg cccccttgtc gacgcggcgg tacggcgtct
     4861 cgaggaatcc gagctcgttc acccgcgcgt aggtcgcgag cgacgagatg agcccgatgt
     4921 tcggcccttc cggcgtctcg atcgggcaca tgcggccgta gtgcgtgtag tgcacgtcgc
     4981 ggacctcgaa ccccgcgcga tcgcgcgtga gacctcccgg cccgagcgcc gagaggcggc
     5041 gcttgtgggt cagctcggcc agcggattcg tctggtccat gaactgcgag agctggctcg
     5101 atccgaagaa gctctggatc acggccgaga tggtccgcgc gttgatgagg tcgtacggcg
     5161 tgacctgctc cgcgtcctgg agcgtcatgc gctcccggat gatccgcgcc attcgcgcga
     5221 gcccgacgtt gaactggttc gcgaggagct cgcccacggc gcgcacccgg cgattcccga
     5281 gatggtcgat gtcgtcggtc gacaccgtga tccccggcac ctccgtcccc aggcgcagag
     5341 ccatgaggta cgcgatgatg acgaggaagt cctcgggacc cagcgtggtc tgattgtcgt
     5401 ccggaacctc gagcgccagc ttcttccgcc ggtccttccc gcccaggatg acgtcgtgcg
     5461 gcagcttccg gttgagcttg tagcggccga ccttcgccag gtcgtagcgc ttcgggttga
     5521 agaagagctt cttgagaatg tcccgcgccg tgtccgccct gggcggctcg cccgggcgaa
     5581 gcaggttgta gatgcgggtg agcgcctcgt cctcggcctt ggtcgaatcc ttcttgagcg
     5641 tgttccggag gatgtccgcc tcgtcgctgt gcgcgatgac gaacgccttc accgtcgaga
     5701 agcccgcctt gcggagcccc tcgatccgct ccatcgagat gacctcgttg gcctcgagga
     5761 ggacctcgcc cgtcttctcg tcgaccacgt cctccgcgga gaccttgccg aggcagtccg
     5821 gctccttctt ggagcgcgcc ccgccgatct cctcctcttc cgtgtcgtag aagagctgga
     5881 ggatctcgcg gtcggacacg tacccgagcg ccttcaggag caccgtcacc ggcagcttcc
     5941 gcttgcggtc gatgtggacg tacatgatgt cgttcacgtc caggctgaac tcgacccagg
     6001 agccgcggta cggaatgatc cgcgccgtga agagcttctt gccgctcgga tgcgacgact
     6061 cgtcgaagaa gacgcccggc gagcggtgga gctggctcac gatgactcgc tcggcgccgt
     6121 tgatgatgaa ggtccccttg tcggtgatga tcggcagctc gccgaggtag acctcctgct
     6181 cgatgatgct cttgtcctta cgcccctcgt ccgtgtcctc gcggatgcgc aggcgaaggg
     6241 tcgccttgag cggaaccgag aaggtcaggt cccgctcctg gcactcgtcc acggagtact
     6301 tgggctcgcc caagtcgtat cgaacgaact cgagcgagaa gaactcgcgc ggatcactga
     6361 tgggaaatac gctctgaaag acctcctgaa gcccttccag cttgcgcttc agtgggtcga
     6421 tatcggcctg aaggaaattc cggaacgaat ccagctgcac gtcgagcagg ttcgggatct
     6481 ggatatccca ctcccgctcg atcttgctga agctccgtcg ctccttgccg cggctcgctt
     6541 tcacgtcgtg tacacccttt cggtaaagcc cttagcgtcg cccgatgagg gccgaatcag
     6601 cggatctcga ccgatgcccc gttctcctcg agcttcgtct tgatcgaccc ggcctcttcc
     6661 ttcgaaacct tctccttcac cggcttgggc gcgccgtcca cgagatcctt cgcctccttg
     6721 agaccaaggc ccgtcacctc gcgcaccacc ttgatgacct ggatcttctt gtcgccggcc
     6781 ccggtcagga cgacgtcgaa ctccgtcttc tcctcgaccg cagccgcggc gccggcgccg
     6841 ccgcccatcc ccggcatggc ggccatcatc ggcatcgccg ccgtcacgcc aaagtgctcc
     6901 tcgagcttct tcacgagacc cgacacctcg agcatcgtca tgccctcgag gagagtgatg
     6961 atctgctgat ccttcgtcgc tgcttccacg ttcgtcttcc tccttcagtt tttccccggg
     7021 cgtcgccgcg ggggatcgga ccgcggctta cgcctgcttc ttcttaccga cggcgtcgac
     7081 gacgcccacg agctgccgca gggatccagc gaggacaccc acgacgccct gcatgggcga
     7141 gcgcagaccg ccgatgaact ggccgaggag cacttcccgc gggggaagcg ccgccaggct
     7201 cttgattcca tccggcccat acacgtggcc ggccacgtac gccgccttca gggccggctt
     7261 ctcgaattcc ttgctgaagt cggcgaggat cttcgccgcg ctcacctcgt ccggggcgaa
     7321 ggcgatgccc gtcggaccct cgaggtagct gtcgagcgag gtcacgccgt gatccttgag
     7381 cgcgcgcttc gtgagcgtgt tcttcgagac gcggtactcc accttcgcgt cacggagctt
     7441 gcgccgcatg cgcgacacga gctccacgtt catgccctgg aaatcggtca ggtagatcga
     7501 tttcgctttg gccagaagct ccgcaagctc gcggacttcc tgttcttttt ctactagggc
     7561 catcgaagtc tccgtttcgg gtccgtcagg gacgggccgg ttaggccttg agtccggcct
     7621 ggatcgctgc gggatccagc cgcaccggcg gtcccatcgt cgagctgagc gtgatgctgc
     7681 gcaggtactg ccccttggcc gcggcgggct tctgccggat cacctcgcgc aggaacactt
     7741 ccacgttctg gacgagctgg tccttcccga aggaggcctt gcccaccggc gcgtggaggt
     7801 tgcccgcctt gtcgacccgg tactcgatct tcccggactt cacctcgcgc acggccttcg
     7861 cgacgtccat cgtcacggtt ccgctgcgcg gattcggcat gaggccgcgc gggcccagga
     7921 cgcgcccgag ctttccgacc tcacccatca tgtccggcgt cgcgatgatg ctgtcgatgt
     7981 cgagccagcc ttcgttcagc cgcttgatga attcctcgga gccggcgtag tccgcgcccg
     8041 cgtccttcgc ctcgcgctcc ttctcgccct tcgtcagcac caggacccgg atcttcttcc
     8101 cggtcccgtg cggcagcacc acggtgccgc gcacctgctg ctcggcgtgg cggggatcga
     8161 cgcccaggct catggacacc tcgacggtct cgtcgaactt ggcggtggcg gacttcttga
     8221 gacgctccac cgcgtcttgg agatcgccca ggcggtccag gccggtcact tcgacggcct
     8281 tcttgaatcg cttcccctgc ttcatcccgc cacctcgatt cccatgctcc tggcggttcc
     8341 ttccaccatg cggatggcgg cctcgaggtc gttggaattg aggtcgggct tcttcagctc
     8401 ggcgatctcg cgaacctgcg cggccgtgac cttggccacc ttgttccggt tcggctcgcc
     8461 cgagcctttc gcgatcccgg cggcgcgctt caggagcacc gcggccggag gcgtcttggt
     8521 gatgaaggtg aacgagcggt cgttgtacac cgtgatcacc acggggatga tgagccccgg
     8581 ctgaccctgg gtccgcgcgt tgaagccctt gcagaactcc atgatgttca cgccgtgctg
     8641 gcccagcgcc ggcccgatgg gcggcgcggg cgtcgcgttg ccggcgggca cctggagctt
     8701 cacgatggcc tgtaccggtt tcgacttcgc catgcctgga ttcctttcgt tcgcgcgggt
     8761 cgccccgctc ccacgtgcgc gtggtggcgc gcggacgccc gaaggggcgc cgcttccggt
     8821 ccgctagacc ggctgaacct ggaggaaatc gagctcgacg ggcgtcgcgc ggccgaagat
     8881 cgagaccatg accttcacct tctggcgctc cgggttcacc tcgtccacca cgccggtgaa
     8941 gtcggtgaac ggcccgtcga cgactttcac gtgctctccg gacttgaacg gcacctcggc
     9001 ggagccgcgc aaacggccgc ccgcctccat gtggccgagg atccgcttca cttccgcttc
     9061 gcgcagagga atcggctcct ggcccgagcc gacgaagcgg gtgacgccag gcacgttctg
     9121 cacgagcagg cgcgtgtcct ggttcaggtc catctcgacg agcacgtacg agggaaacgt
     9181 cttgcgcttc gacgtgatgc gcttgccgtc cttcatctcg gcgaactcct cggttgccac
     9241 gaggatttgg ccgaaattcg gctccagccc ggcggagtgg atcgcccgct cgaggttggc
     9301 cttcaccttg ttctcatgac ccgaataggt atggatcaca taccactgca tgggccgtcc
     9361 ttccccttac ccgaccatct tgacgagcgc ttcgaacgcg agcgagagcc cccggtcgat
     9421 gacgccgatg aacaccatca tcaggaacac catgatgatg accacgaggg tcgactcgcg
     9481 gagctcctcg cgcgtcggcc agctgacctt ggacatctcc acccggacgt ccttgacgta
     9541 atccgtgagg ctttgaacgg ggcccgtccg gacttccggt ttttcgctcg tcgtctctgc
     9601 gcgcacgatt tcgcctcttg cggtgccttc gttggtggca ggccaggagg gattcgaacc
     9661 cccgacaccc ggttttggag accggtgctc taaccggact gagctactgg ccttccggtc
     9721 ccggcccggc gtctaccggg tctctttgtg aagcgtgtgc ttccggcagc gcgggcagaa
     9781 cttcttccac tccacgcgat ccggatgctt ccgcttgttc ttcgtgttgg tgtagttgcg
     9841 ctctttgcag tcgttgcagg ccagcgtgat gaggtctcgc atcgtcttac tccgtcactt
     9901 ccgcgacgac gccggcgccg acagtgcggc cgccctcacg gatggcgaag cgaagctcct
     9961 tctccatcgc gatcggcgtg atcagctcca ccgccatctc cacgttgtcc cccggcatca
    10021 ccatctcccg accctcgggc aacgtcgcca cacccgtcac gtcc
//
LOCUS       DASTKC010000069        10542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1942553, whole genome shotgun sequence.
ACCESSION   DASTKC010000069 DASTKC010000000
VERSION     DASTKC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942553"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VFR25_03050"
     CDS             complement(<1..1435)
                     /locus_tag="VFR25_03050"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9940056.1"
                     /translation="MSSTLEPLLRPKSVAVIGASRRRDSIGGAILHNLLEQEFQGPVY
                     PVNPSADHVQSVASYASVDAIPGPVDLAVVVVPSAHVLEVVEACGRKGVRALVVISAG
                     FKEIGPEGAKREADLRAAVKKHGMRLIGPNCLGILNTDPNVRLNATFAPVEPPHGRVA
                     FSSQSGALGLAILSYARELNLGISQFVSVGNKADVSGNDLIEFWDEDDGTDVILLYLE
                     SFGNPAKFTQLARRVARRKPIVAVKSGRTVSGARAASSHTGSLAGSDAAVAALFQQSG
                     VIRTDTIEELFDTALLLAYQPVPKGPRVAILTNAGGPGIMAADACESAGLDLPSLAPA
                     TVKALKSFLPAEASVRNPVDMIASADAPSYEKALRLLVADENVDSVIVIFVPPLVTGA
                     TDVARAILAGGKGSTKPILSCFMGSHGIPESLKSLNEGHIPSYAFPEAAARTVARVAK
                     YGVWRAAKPGEVPKLKGVDPAKARAAIE"
     gene            complement(1456..1755)
                     /locus_tag="VFR25_03055"
     CDS             complement(1456..1755)
                     /locus_tag="VFR25_03055"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9940057.1"
                     /translation="MSKLSEPEIREAIQELAGWVDQDGKLTKTFTHSSFPEAIVFVNA
                     VAHIAELANHHPDIDVRYSNITLSLVTHDEGGITPRDVALARQVEAIRRKAGVPA"
     gene            complement(1765..2052)
                     /locus_tag="VFR25_03060"
     CDS             complement(1765..2052)
                     /locus_tag="VFR25_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET9940058.1"
                     /translation="MATDEEIKTALEEVVDPEIHLSIVELGLVREIDQSADPVVIRML
                     LTTPFCPYAPQIVAEVKAVVTRVTEKGTEVEILPEQWTPEMMPDPGLLGRW"
     gene            complement(2072..2398)
                     /locus_tag="VFR25_03065"
     CDS             complement(2072..2398)
                     /locus_tag="VFR25_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET9940059.1"
                     /translation="MTETATRFVRVGRAADVPPGGVEVFDVEDRKIAVYRLEDGYYAI
                     EDICTHDGGPLAEGEIDGDQVICPRHGARFSIRTGAALTFPAITPVEHYPVRVDGDDL
                     LVGLPD"
     gene            complement(2395..2799)
                     /locus_tag="VFR25_03070"
     CDS             complement(2395..2799)
                     /locus_tag="VFR25_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9940060.1"
                     /translation="MSFDDLYRQNILDHYQNPRNFGTLEHPDISAEDSNPLCGDEIRI
                     DLKVKDGVIEDVRFSGKGCSISRAAASMLTEEIRGKTLEEVKRIGRDEVLEMLGIELG
                     PVRLKCALLALKTLKVGVYGIRGWPGDDEEDR"
     gene            complement(2796..4037)
                     /locus_tag="VFR25_03075"
     CDS             complement(2796..4037)
                     /locus_tag="VFR25_03075"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET9940061.1"
                     /translation="MKTLAPVDFAAIRRQFPIFDRKIHGHPLIYLDSTATTQKPVAVL
                     DALERYYRTYNANIHRGVYVIGEEATQAYEDARGKVAKFLNASSHREIVFTRGTTESV
                     NLVAHGWGRKFVNQGDVVLLTEMEHHSNLVPWQLLAQDRGATLRFIPIDGHGRLDLTN
                     LDRLLEGPVKIVSLAHVSNVLGTINPVSEVIRRAHAVGARVMLDAAQSVPHMPVDVRA
                     LDCDFLALSGHKMLGPTGIGVLYGKREALESMNPFLGGGEMIREVRLEGSTWNDVPFR
                     FEAGTMNIADTIAFGVAIDYLEGIGMANVEAHGRELVEDTFRVLGSIPGVTIYGPPAN
                     EPRGAIVAFTVDDIHAHDVAAALDREGVAVRAGHHCAMPLHTKLGVAATSRASYHVYN
                     VPSDIERLGDGIRKAKQLFHR"
     gene            complement(4034..5401)
                     /gene="sufD"
                     /locus_tag="VFR25_03080"
     CDS             complement(4034..5401)
                     /gene="sufD"
                     /locus_tag="VFR25_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET9940062.1"
                     /translation="MNPADTLEPARLDEAIALGSIKADDIAARSARDGEPGFLARRRA
                     EAWAFAEKTPFPGRLEELWRRTDLSGAGWDKVLVDRATTPSVLALADLPTPIQHEIGP
                     ASERSALLVQVDSGIAYREVDEDLARRGVTIMSLAEMAKRQPALLELTLGSTVRFGET
                     RFTAIQAALASGGAVVYVPRGVEVERPIRILIAREASGHATFPHVLVALEPQAKATVI
                     EEYFSPGDSGAGVNVGVTEVTVGEGADLHMATLQRWGSAVVHVGTERVRVARDARFHW
                     TFAGLGGKVTKIDMEMHLEGEGSEARFSGCYFGDGSQHFDFHSFQNHVKGHSTSDLLF
                     KGALRGKARTVYQGLIKVHKDAQRSDAYQANRNLILSDKARADSIPSLEIEANDVRCT
                     HGATVGQIDEEQRFYLMSRGLDRHEAERLILQGFFEPVLERIPAESLRAIVTEAVERK
                     AAIEA"
     gene            complement(5445..6857)
                     /gene="sufB"
                     /locus_tag="VFR25_03085"
     CDS             complement(5445..6857)
                     /gene="sufB"
                     /locus_tag="VFR25_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9940063.1"
                     /translation="MNEQQQTLDIREGYKEKFGFHDDIAPVFKSRRGLDKSVVAEISA
                     MKKEPAWMLDFRLKALEQFEKKPLPKWGGNVGEIDFQNIFYYLKPSEGETKNWDEVPA
                     DIKRTFDKLGIPEAEQKFLSGVGAQYDSETVYHKIKQDLADKGVIFLDTGSALEQHPD
                     LFRQYFGTIIPPSDNKFAALNSAVWSGGSFIYVPKGVKIDMPLQAYFRINAQNMGQFE
                     RTLIIVDEGAWVHYVEGCTAPIYASDSLHSAVVEIIVKKGARCRYTTIQNWSNNVFNL
                     VTKRAAAYQDATMEWVDANLGSKLTMKYPSVYLMEPGAHAEILSIAFAGKGQHQDAGG
                     KAVHGAPNTTSSVVSKSISKDGGRAGYRGLIKIAKGAKGSKSTVRCDALLLDEHSRSD
                     TYPYMEIEEDQVTIGHEATVSKIGDEQLFYLMSRGIPEAEAAAMIVSGFIEPITKELP
                     MEYAVEMNRLIQLQMEGSVG"
     gene            complement(6882..7655)
                     /gene="sufC"
                     /locus_tag="VFR25_03090"
     CDS             complement(6882..7655)
                     /gene="sufC"
                     /locus_tag="VFR25_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET9940064.1"
                     /translation="MVSAKPTLEIRDLHVAVEGKPILKGLSLAVSKGEVHALMGPNGS
                     GKSTLANALMGNPKYEITSGSILLNGVDLLELSADERGRKGLFLAFQYPTAIPGVSVA
                     NFLRMALTARRKEMGIEKPLPPKEFRALVKEKMALLKMDDSFAGRYINDGFSGGEKKR
                     AEILQMAVLQPEIAILDETDSGLDIDALRIVSEGVNALSGPNLGVLLITHYQRILNYI
                     KPQFVHVLVDGRIVKSGGPELAHELEAQGYDWVREIQAA"
     gene            complement(7728..8096)
                     /locus_tag="VFR25_03095"
     CDS             complement(7728..8096)
                     /locus_tag="VFR25_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9940065.1"
                     /translation="MSQTNEKQVPAAQPETLAVTPAAVEWVKRIRTKEGKEGQALRLG
                     VKAGGCSGYSYFMGFTTSERSGDLVLQYDGLTVYVDPRSLEILRGTTVDFARGMLGSG
                     IQFNNPRVKKSCGCGESFSL"
     gene            complement(8093..8617)
                     /locus_tag="VFR25_03100"
     CDS             complement(8093..8617)
                     /locus_tag="VFR25_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9940066.1"
                     /translation="MSKLLKIGDLARRTGKSVRALHLYEELGLLTPTARSQGGFRLYD
                     DTAETRIRWIELLQDSGFSLHEIQALLRAWRSTRYGPDAMARIREIFEKRLAETKQAI
                     VRYQALERELQGSLEYLGACLECRPPRTTQDDCPHCPVDHGMTREPALVAGFHTGPPT
                     PGMIPLHATSENGS"
     gene            8878..9276
                     /locus_tag="VFR25_03105"
     CDS             8878..9276
                     /locus_tag="VFR25_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HET9940067.1"
                     /translation="MRLAPRVLGVTTLLTALLAVLALSGLSCGGDDNNPMNPGGGGGA
                     DVTITINGMNGANSYSPNPDTVTVGQTVAWHNADATTHTATANGAAFNTGNISGGSTS
                     AKITMGTVGSFPYHCSIHPTMTGTLVVVVP"
     gene            complement(9281..>10542)
                     /locus_tag="VFR25_03110"
     CDS             complement(9281..>10542)
                     /locus_tag="VFR25_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HET9940068.1"
                     /translation="DRKDSARREAVESLATFGRFTAAMLDRSIDDAFQPLARNGLTRW
                     IVTELGSVQVLDRPAEGTRGTIRRAHGPEWMLQIYAGNVPTLPVWPIYSALILKAALL
                     GKTSAREPLFAPLLARTVAEVDEKLGACLAVVWWKGGTGGLDRTALSAAPAVLAFGGE
                     EAVGSVGRQAMQGASVVLHGPKVSVGYVERRALTFAGIRGLAARAAHDVSMYDQDGCL
                     SPHAFYVERRGQVSPEKFGEALAAALEEASKDLPPVVAEEEEAAGVQLARAQARFESA
                     GIATVTRVLESPGSTSWTVIVDDRARFEPGPTRRTIRVHAIDGPDEVARVLASSARSI
                     EALGLEARGPERVKLAARFAELGIPRIAAVGSLQNPVPWGTHGGVPRLLPFVTWTTVD
                     QVGGTSGPAKAPGAVRRRLRAAGIRRR"
BASE COUNT         1614 a         3594 c         3489 g         1845 t
ORIGIN      
        1 gctcaatggc ggcgcgagcc ttcgcgggat cgacgccctt cagcttcggc acctcgcccg
       61 gtttggccgc ccgccacacg ccgtacttcg cgacgcgggc cacggtgcgc gccgccgcct
      121 cggggaacgc gtacgacgga atgtgcccct cgttgagcga tttcaggctc tccggaatgc
      181 cgtgcgatcc catgaagcag gagaggatcg gcttggtgct ccccttccct ccggccagga
      241 tggcgcgcgc cacgtcggtg gcacccgtca cgagcggtgg gacgaagatc acgatgacgg
      301 aatccacgtt ctcgtcggcg accaggagcc tcagggcctt ctcgtaggaa ggcgcatccg
      361 ccgaggcgat catgtcgacc ggattcctca cactcgcctc ggccggcagg aacgacttca
      421 aagccttcac ggtcgccggg gccagcgacg ggagatccag gcccgccgac tcgcacgcgt
      481 cggccgccat gattccggga ccgcccgcgt tcgtgagaat ggcgacgcgc ggtcccttcg
      541 gcaccggctg gtaggcaagg aggagcgccg tgtcgaagag ctcttcgatc gtgtcggtac
      601 gaatcacgcc gctctgctgg aagagcgcgg ccaccgcggc atcggagccc gccagcgatc
      661 cggtgtggga cgacgccgcg cgagccccgc tcaccgtgcg cccgctcttc accgccacga
      721 tcggcttccg ccgtgcgacg cgccgggcga gctgggtgaa cttggccgga ttgccgaacg
      781 actcgaggta gagaaggatg acgtcggtgc cgtcatcctc gtcccagaac tcgatgagat
      841 cgttgccgga gacgtcagcc ttgttcccca cgctcacgaa ctgcgagatg ccgaggttga
      901 gctcgcgggc gtaggagagg atcgcgaggc ccagcgctcc gctctgcgag gagaaggcca
      961 cgcgcccgtg gggcggctcc accggcgcga acgtggcgtt gagccgcacg ttcgggtcgg
     1021 tgttcaggat gccgaggcag ttggggccga tcagacgcat cccgtgcttc ttcacggcgg
     1081 cgcgcaggtc cgcttcgcgc ttcgctccct cggggccgat ctccttgaag cccgccgaga
     1141 tcacgaccag cgcgcgcacg cctttgcgcc cgcacgcttc gaccacctcg aggacgtgcg
     1201 ccgacggcac cacgacgacc gccagatcga cggggcccgg gatcgcgtcg acggaagcgt
     1261 acgaggcgac cgactggacg tgatccgcgc tgggattgac cggatagacc ggaccctgga
     1321 actcctgctc gagcaggtta tggaggatcg cgcccccgat cgagtcgcgt ctgcgggagg
     1381 ctccgatcac ggccacggac ttgggccgga ggagcggctc gagcgtgctc gacatgcggc
     1441 gagcgtggcc cgccgctatg cgggcacgcc ggccttgcgg cggatcgcct ccacctgtcg
     1501 ggccagcgcg acgtccctag gggtaattcc cccttcgtcg tgggtcacga gcgagagcgt
     1561 gatgttggaa tagcgcacgt cgatgtccgg gtggtgattc gccagctcgg cgatgtgcgc
     1621 gaccgcgttg acgaagacga tcgcttccgg gaacgacgag tgcgtgaagg tcttggtcag
     1681 cttgccgtcc tggtcgaccc aaccggccag ctcctggatg gcttcgcgaa tttcgggctc
     1741 ggacagtttg ctcatgtggc cctcctacca gcggccgagc agacccgggt cgggcatcat
     1801 ctccggggtc cactgctcgg gcaggatttc cacttccgtc cccttctcgg tgacgcgggt
     1861 gacgactgcc ttcacctccg cgacgatctg gggcgcgtag ggacagaagg gggttgtcag
     1921 gagcatgcgg atcacgacgg gatcggcgct ctggtcgatc tcgcggacga gtcccagctc
     1981 cacgatgctc aggtggatct ccggatccac cacttcctcg agcgcggtct tgatttcttc
     2041 gtcggtagcc atggtcgctc ctcttaggac ttcagtcggg caagccgacc aggaggtcgt
     2101 ctccatcgac ccgcaccggg tagtgctcca cgggcgtgat cgcggggaag gtgagggcgg
     2161 ctccggtcct gatgctgaag cgcgccccgt ggcgggggca gatcacctgg tcgccgtcga
     2221 tctcgccttc ggcgagcggc cccccgtcgt gggtgcagat gtcctcgatc gcgtaatagc
     2281 cgtcctcgag acggtagaca gcgatcttac ggtcctccac gtcgaacact tcgacgccgc
     2341 ccggaggcac gtcggcggcg cgtcccaccc ggacgaaccg ggtcgcggtc tccgtcatcg
     2401 gtcctcctcg tcgtcgccgg gccagccccg gattccatag actcccactt tcagcgtctt
     2461 gagcgcaagc agcgcgcact tgagccggac cggtcccagc tcgatgccca gcatctcgag
     2521 cacctcgtcc ctgccgatcc gcttgacctc ttcgagagtc ttcccgcgga tctcctccgt
     2581 gagcatggac gcggccgcgc ggctgatgga gcatcccttg cccgagaacc gcacgtcctc
     2641 gatgacgccg tccttcacct tgagatcgat ccggatctcg tccccgcaga gcgggttcga
     2701 atcctcggcg ctgatgtccg gatgctcgag cgtcccgaag ttacggggat tttggtagtg
     2761 gtcgaggatg ttctggcggt agaggtcgtc gaagctcatc ggtggaagag ctgcttcgcc
     2821 ttccggatgc cgtcgccgag ccgctcgatg tcggagggca cgttgtagac gtggtagctc
     2881 gcgcgcgacg tcgccgcgac gccgagcttc gtgtggagcg gcatcgcgca gtgatgcccc
     2941 gcgcggacgg ccacgccctc gcggtcgagc gccgccgcca cgtcgtgcgc gtggatgtcg
     3001 tcgacggtga acgccacgat ggcgcctcgc ggctcgttcg cgggcggccc gtagatcgtc
     3061 acgccgggaa tcgatccgag gacccggaag gtatcctcga ccagctcgcg cccgtgcgct
     3121 tcgacgttcg ccatcccgat cccttcgagg tagtcgatcg ctacgccaaa cgcgatcgta
     3181 tccgcgatgt tcatcgtgcc cgcctcgaag cggaagggca cgtcgttcca ggtcgagccc
     3241 tcgagcctca cctcgcggat catctcgccg ccccccagga acggattcat ggattcgagc
     3301 gcctcgcgct tgccgtagag gacgccgatc ccggtcgggc cgagcatctt gtggccggag
     3361 agcgcgagga agtcgcagtc gagggcgcgc acgtcgacgg gcatgtgggg cacgctctgc
     3421 gcagcgtcca gcatcactcg cgcgcctacg gcatgcgcac ggcggatgac ctcggagacc
     3481 ggattgatcg tgccgagcac gttcgagacg tgcgcgagcg agacgatctt caccggcccc
     3541 tcgaggagcc ggtccagatt cgtgagatcg agccgcccgt ggccgtcgat cgggatgaag
     3601 cggagcgtcg ctccgcggtc ctgcgcgagg agctgccacg ggacgagatt cgagtggtgc
     3661 tccatctcgg tgagcagcac cacgtcgccc tggttgacga atttccgccc ccagccgtgc
     3721 gccacgaggt tcaccgactc ggtcgttccg cgcgtgaaga cgatctcgcg gtgcgaggag
     3781 gcgttcagga acttggccac cttgccgcgc gcgtcttcgt aggcctgcgt cgcctcttcg
     3841 ccgatgacgt acacgccgcg atggatgttg gcgttgtagg tgcggtagta gcgctcgagc
     3901 gcatccagga cggcgaccgg cttctgcgtg gtcgccgtgg agtcgagata gatgagcgga
     3961 tgcccgtgga tcttccggtc gaagatcggg aactggcggc ggatcgccgc gaagtcgacc
     4021 ggcgccaggg tcttcatgct tcgatcgccg ccttccgctc gaccgcctcg gtcacgatgg
     4081 cccgaagcga ctcggccggg atccgctcga gcaccggctc gaagaacccc tggaggatga
     4141 gccgctcggc ttcgtgccga tcgagcccgc gcgacatgag gtagaagcgc tgctcctcgt
     4201 cgatctggcc cacggtcgct ccgtgcgtgc agcggacgtc gttggcctcg atctcgaggc
     4261 tgggaatgga gtcggcgcgc gccttgtcgg agaggatgag gttccggttc gcctggtagg
     4321 cgtccgagcg ctgcgcgtcc ttgtggacct tgatgagccc ctggtacacg gtccgggcct
     4381 tcccgcgaag cgctcccttg aagaggaggt cgctcgtcga gtgtcccttc acgtggttct
     4441 ggaagctgtg gaagtcgaag tgctgcgacc cgtcgccgaa gtagcagccc gagaagcgcg
     4501 cctcgctccc ttcgccctcc aggtgcatct ccatgtcgat cttggtgacc ttgccgccga
     4561 gtcccgcgaa ggtccagtgg aagcgcgcgt cgcgcgccac ccgcacccgc tcggtgccga
     4621 cgtgcaccac cgccgagccc cagcgctgga gcgtggccat gtggagatcc gccccctcac
     4681 ccaccgtgac ctcggtcacg ccgacgttga cgccggcgcc cgagtcaccg ggcgagaagt
     4741 actcctcgat cacggtcgcc ttggcctgcg gctcgagcgc cacgaggacg tgcgggaacg
     4801 tggcatgccc cgacgcctcg cgcgcgatca ggatgcggat cggccgctcg acctcgacgc
     4861 cgcgcggcac gtagaccacg gcaccacccg acgcgagcgc cgcttgaatg gcggtgaagc
     4921 gcgtctcgcc gaagcgcacc gtgctcccga gggtgagctc gaggagcgcc ggctggcgct
     4981 tcgccatctc ggcgagcgac atgatggtca cgccgcggcg cgccagatcc tcatcgacct
     5041 cgcggtatgc gattccggaa tcgacctgaa cgaggagagc ggagcgctcg gatgcgggtc
     5101 cgatctcatg ctggatggga gttgggagat ccgcgagcgc aagaacggac ggcgtggtcg
     5161 cgcggtcgac gaggactttg tcccagcctg cgccgctcag atcggtgcgc cgccacagct
     5221 cctcgagacg gccggggaac ggcgtcttct cggcaaaggc ccacgcctcg gccctgcggc
     5281 gggccaggaa tccaggctcg ccgtcgcgcg cagagcgcgc cgcgatgtcg tcggccttga
     5341 tggacccgag cgcgatcgct tcgtcgagcc gcgccggctc cagggtatcg gcaggattca
     5401 cgtgttgttc tctccgttcc ggtaagggac cgtgtccggc ggcctcagcc gaccgaaccc
     5461 tccatctgaa gctggatgag ccggttcatc tcgacggcgt actccatggg cagctccttc
     5521 gtgatgggct cgatgaaccc gctcacgatc atggcggccg cctccgcctc gggaatgccg
     5581 cgactcatga gatagaagag ctgttcgtcg ccgatcttcg agacggtcgc ctcgtggccg
     5641 atcgtgacct ggtcctcctc gatctccatg tacgggtacg tgtcggaccg ggaatgttcg
     5701 tcgaggagga gcgcgtcgca gcgcaccgtc gacttggatc ccttggcgcc tttggcaatc
     5761 ttgatgagcc cgcgatagcc ggcccgtccg ccatccttgc tgatcgactt cgagaccacg
     5821 ctcgaggtcg tgttgggcgc gccgtgcacc gccttgccgc cggcatcctg atgctgcccc
     5881 ttcccggcga acgcgatcga aaggatctcg gcgtgggcgc ccggctccat gagatagacg
     5941 ctcgggtact tcatcgtgag cttggagccg agattggcgt cgacccactc catggtggcg
     6001 tcctgatagg ccgcggcccg cttggtcacg agattgaaca cgttgttgga ccagttctgg
     6061 atggtcgtgt agcggcagcg ggcgcccttc ttgacgatga tctccacgac cgccgagtgc
     6121 agcgaatcgc tcgcgtagat cggcgccgtg cagccctcga cgtagtgcac ccacgcaccc
     6181 tcgtcgacga tgatgagcgt ccgctcgaac tgccccatgt tctgggcgtt gatccggaag
     6241 taggcctgga gcggcatgtc gatcttcacg cccttcggca cgtagatgaa cgagcctccg
     6301 ctccacacgg cgctgttcag cgcggcgaac ttgttgtcgc tcggtgggat gatcgtcccg
     6361 aagtactggc ggaacagatc cggatgctgc tcgagggccg accccgtgtc gaggaagatg
     6421 acccccttgt ccgccaggtc ctgcttgatc ttgtggtaca ccgtttccga gtcgtactgg
     6481 gccccgacgc cggagaggaa tttctgctcc gcttcgggaa tgccgagctt gtcgaacgtg
     6541 cgcttgatgt ccgcggggac ctcgtcccag ttcttggtct ctccctcgct cggcttcagg
     6601 tagtagaaga tgttctggaa gtcgatctcc ccgacgttgc cgccccactt ggggagcggc
     6661 ttcttctcga actgctcgag cgccttgagg cggaagtcga gcatccaggc cggctccttc
     6721 ttcatggcgg agatctccgc gacgacgctc ttgtcgagac cacgccggct cttgaagacc
     6781 ggcgcgatgt cgtcgtggaa cccgaacttc tccttgtagc cttcccggat atcaagtgtc
     6841 tgctgctgct cgttcatgac ttgagcctcc tcgtccgcac gctaggccgc ctggatctcg
     6901 cggacccagt cgtaaccctg ggcctcgagc tcatgggcca gctccggccc gccggacttc
     6961 acgatccgcc cgtcgaccag aacatggacg aactgcggct tgatgtagtt caggatccgc
     7021 tggtaatgcg tgatcaggag cacgcccagg ttcggaccgg agagcgcgtt cacgccctcg
     7081 gacacgatgc ggagcgcgtc gatgtcgagc cctgaatcgg tctcgtcgag gatggcgatc
     7141 tccggctgga gcaccgccat ctggaggatc tcggcgcgct tcttctcgcc gcccgagaac
     7201 ccgtcgttga tgtatcgccc cgcgaaggag tcgtccatct tgagaagggc catcttctcc
     7261 ttcacgaggg cccggaattc cttcggcggg agcggcttct cgatccccat ctccttccgc
     7321 cgcgcggtga gcgccatgcg gaggaagttg gccacgctca cgcccgggat cgcagtcgga
     7381 tactggaacg cgaggaagag ccccttgcgg ccacgctcgt ccgcgctcag ctcgaggagg
     7441 tcgaccccgt tcaggagaat gctccccgag gtgatctcgt acttcggatt ccccatcagc
     7501 gcgttcgcga gcgtgctctt gcccgagccg ttgggtccca tcagggcgtg cacttctccc
     7561 ttgctcacgg cgagggagag gcccttcaag atgggctttc cttcgacggc cacgtgtaga
     7621 tcccggattt cgagcgtcgg cttcgcgctt accatggtct ccttccccta gtatcggtcg
     7681 cggctctcac cgcgcccctg tgatcacgga ccccgaggtc cgcgccgtca tagactgaag
     7741 ctctccccgc agccgcacga cttcttgacg cgggggttgt tgaactggat gccgctcccg
     7801 agcatgccgc gcgcgaagtc cacggtcgtg ccgcggagga tctcgagcga gcgcgggtcc
     7861 acgtagacgg tgagtccgtc gtactggagc acgaggtcgc ccgagcgctc cgacgtggtg
     7921 aatcccatga agtagctgta tcccgagcag ccccccgcct tcactccgag gcggagcgcc
     7981 tgtccttcct ttccttcctt ggtccggatc cgcttcaccc actcgaccgc cgccggagtc
     8041 acggcgaggg tttcgggctg ggcggccggg acctgcttct cgttcgtctg gctcacgatc
     8101 cgttctccga agtcgcgtgg agcgggatca tccccggggt cggcggaccc gtgtgaaatc
     8161 cggcaacgag cgcgggctcc cgcgtcatgc cgtgatccac agggcagtgc ggacaatcgt
     8221 cctgcgtcgt gcgcggcggc cggcactcca ggcacgcacc caggtactcg agcgatccct
     8281 ggagctcgcg ctcgagcgcc tggtaccgca cgatcgcctg cttggtctcg gccaggcgct
     8341 tctcgaagat ctcccggatc cgagccatcg catccgggcc gtagcgtgtg ctccgccagg
     8401 cccggaggag cgcctggatt tcgtgaagcg agaagccgct gtcttgcaga agctcgatcc
     8461 agcggatcct ggtctcggcc gtgtcgtcgt agaggcggaa gccgccctgc gaccgcgcgg
     8521 tcggggtcag gagcccaagc tcctcataga ggtggagcgc gcggaccgac ttcccggtcc
     8581 ggcgcgcgag atcgccgatc ttcagaagct tgctcatcgt ctccaagtct cgttgccctc
     8641 cttaccggat cgtaccctta cgcgcacgta agagtccagg aaaaaggggc cggctgggtg
     8701 ggatcgccca tccaggcctc ggcgggccta cggggttgta acaatttggg ttatcggtgg
     8761 actctcccct aggagactat gcatcccaag ggaacaagcg agggtcgccc ggtgtgccca
     8821 cgggcatccc tctgtctttg atgggtggcc ggtcctaacg atcctaggag gtgcctcttg
     8881 cgtctagctc cccgagtcct cggcgtcacg acccttctca cggccctgct ggccgtcctc
     8941 gccttgagcg gcttgagctg cggcggcgac gacaacaacc cgatgaatcc aggcggtggg
     9001 ggcggggccg acgtcacgat cacgatcaac gggatgaacg gggccaattc ctactccccc
     9061 aatcccgaca ccgtgaccgt gggccagacc gttgcgtggc acaacgccga cgcgacgacc
     9121 cataccgcga ccgcaaacgg cgccgccttc aatacgggga acatcagcgg cggcagcacg
     9181 agcgccaaaa tcacgatggg cacggtgggt tcgttcccct accactgctc gatccacccg
     9241 acgatgacgg gaacgctggt tgtggtggtg ccctagccga tcagcggcga cgaatccccg
     9301 cggcgcgaag ccggcgccga accgctccgg gagccttggc ggggcccgaa gtcccgccca
     9361 cctgatccac ggtcgtccaa gtgacgaagg ggagtaggcg gggcacgccg ccgtgcgtgc
     9421 cccacgggac ggggttctgg agcgagccga ccgccgcgat cctcgggatc ccgagctcgg
     9481 cgaagcgtgc ggcgagcttc acgcgctccg gccccctcgc ctcgagtccc agggcctcga
     9541 tcgagcgcgc cgaggacgca agcacgcgag cgacctcgtc cggtccgtcg atcgcgtgca
     9601 cgcggatcgt gcgccgggtg ggccccggct cgaagcgggc gcggtcgtcc acgatgacgg
     9661 tccacgaggt cgagccgggc gattcgagca cgcgcgtgac cgtcgcgatg cccgcggact
     9721 cgaagcgcgc ctgggcccgc gcgagctgaa cccccgcggc ttcctcttct tccgcgacca
     9781 cgggcggcag atccttcgac gcctcctcga gcgcggcggc gagcgcctcg ccgaacttct
     9841 ccggcgacac ttgcccccgc cgctccacgt agaacgcatg cggtgacagg cagccatcct
     9901 ggtcgtacat cgagacgtcg tgggcggccc gcgcggcgag gccgcgaatt cccgcgaacg
     9961 tgagcgcgcg ccgctcgacg tagccgaccg agaccttggg cccgtgcagc acgaccgatg
    10021 ccccctgcat cgcctgacga cccacgctgc ccaccgcttc ctcgccgccg aacgcgagca
    10081 cggcgggcgc cgccgagagc gcggtgcggt cgagcccgcc cgttcctccc ttccaccaca
    10141 cgaccgcgag gcatgcgccc agcttctcgt ccacctcggc gacggtccgc gcgaggagcg
    10201 gcgcgaagag cggttcgcgc gccgaggtct ttccgaggag cgccgctttc aggatgagcg
    10261 ccgagtagat gggccacacg gggagcgtcg gcacgttgcc ggcatagatc tggagcatcc
    10321 actcgggccc gtgcgcgcgg cggatcgtgc cccgcgttcc ctccgcggga cgatcgagca
    10381 cctgcaccga tcccagctcc gtcacgatcc atcgcgtgag gccgttccgc gcgagcggct
    10441 ggaaggcgtc gtcgatcgat cggtcgagca tggccgcggt gaatcgcccg aacgtcgcga
    10501 gcgactcgac cgcctcgcgg cgcgccgagt ccttccgatc ga
//
LOCUS       DASTKC010000070         9737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1233890, whole genome shotgun sequence.
ACCESSION   DASTKC010000070 DASTKC010000000
VERSION     DASTKC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233890"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFR25_03115"
     CDS             complement(<1..234)
                     /locus_tag="VFR25_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HET9940069.1"
                     /translation="MTPHDARAEHDTDAPEDEWVLKLYVAGQTPRSIAALENLKSICE
                     GHLGGKYTIEVIDLAQNPRLARTDQIVAVPTLVR"
     gene            complement(231..1964)
                     /gene="kaiC"
                     /locus_tag="VFR25_03120"
     CDS             complement(231..1964)
                     /gene="kaiC"
                     /locus_tag="VFR25_03120"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HET9940070.1"
                     /translation="MATTFQSPNAALLGLEKCPTGIDGLDEITNGGLPRGRPTLVAGG
                     PGCGKTLLGIEFVVRGILRYGEPGVILALEERAEDLVRNVRSLGYDLDALVEEGKLVV
                     DYVQVDPFEIEENGEYDLEGLFLRLGDAIDSVGAKRVSLDTLEVLFTSFKNLGVVRAE
                     LRRLFQWLKDREVTAIVTAERGDGPLTRNGLEEYVSDCVILLDHRVRQQILTRMLRVV
                     KYRGSKHATNEFPFYIDEHGIHVLPITSAGLAHKVSSERIQTGIPRLDDMLQGGPHRG
                     SSILVTGSAGTGKTTLLTHLAHASCVRGERALYFSFEESPAQIVRNMRSVGLEIGPFI
                     EQDLLRIVSARPTMYGLETHLAITHQRVEEFAPRMVIFDPISNLNDVSNDADTKSMFI
                     RLIDFLKERSITVVLADLAARGAETTDVGISSLIDTWILLRDIESSGERNRGLYVLKS
                     RGTAHSNQIREFLITERGVHLQDVYTGPEGVLTGSARASREAEERAEAVVRQQSLERM
                     RRDLEAKRLALDAQIEALRAQYAAEQNEITIRIDQESSRLRELALGRQAMGARRGEDG
                     PSEGPGGNGVP"
     gene            complement(2235..2525)
                     /locus_tag="VFR25_03125"
     CDS             complement(2235..2525)
                     /locus_tag="VFR25_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940071.1"
                     /translation="MIESSTQPRTEGPVAKAIEHQTSKIPSDLFLWAALGFLGVSAVR
                     SIRSRRGGGSGENFISSLGPTFLMLGVYNKIVKLHGSDKYDEERQLAGQYVG"
     gene            complement(2656..2838)
                     /locus_tag="VFR25_03130"
     CDS             complement(2656..2838)
                     /locus_tag="VFR25_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940072.1"
                     /translation="MDRDRDNKRDDDRDDDVQEDDRGTSSTSGRTGSSRNAGSNDREN
                     SRTGNPPGTRQSNKKK"
     gene            complement(3073..3993)
                     /locus_tag="VFR25_03135"
     CDS             complement(3073..3993)
                     /locus_tag="VFR25_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9940073.1"
                     /translation="MIVHLIDGTYELFRHFYGLRRFTKGEDKPFGGVTGVLGTVLQMI
                     EKGATHVGVATDHVIESFRNDLWDGYKTGEGIDRALWAQFHPLEDALLAMGVAVWPMI
                     ELEADDGLASAAAIAERDKRVEKVCIWSPDKDLAQCVRGDRVVQMDRRADRMRDEAGV
                     KEAFGVEPVRIPDFLALVGDSADGYPGIKGIGAVGAARLIARYGPIESFPEEALGAAD
                     RKRALLFKDLATLRTDAPLFGDVEDLEWRGPTAGFEAMAGRMGADRLAERARKAAPAD
                     VPAAARPAAPRGRAPARARRPSRGRGPRAG"
     gene            complement(3990..5273)
                     /locus_tag="VFR25_03140"
     CDS             complement(3990..5273)
                     /locus_tag="VFR25_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9940074.1"
                     /translation="MATDTHRAIEAVWRIEAPRLIGALTRVVRDVGLAEDLAQDALLA
                     ALERWPETGVPDNPAAWLMAAAKHKAIDRLRREKRLEQKHAEIERALESSRDGDRDRL
                     DASLDDPVGDDLLRLIFIACHPVLPPEARVALTLRLLGGLSVPEIARAFLVPEATVAQ
                     RIVRAKRTLADANVPFEVPRKDELEARLHSVLEVIYLVFNEGYSATAGEDWMRPALCE
                     DALRLGRILAELLPEEAEVHGLVALLEIQASRAPARVGPTGEPILLLDQDRSRWDPLL
                     IRRGLAALERAETLGGAEGAYALQAAIAACHARARAADETDWARIAALYGRLAERTPS
                     PVIELNRAVAIGMAHGPAAGLEHVDAIAEEPALRAYHLLPSVRGDLLAKLGRREEARA
                     EFERAAAMTRNDRERRLLLDRAARCASAEGASATG"
     gene            complement(5320..5742)
                     /locus_tag="VFR25_03145"
     CDS             complement(5320..5742)
                     /locus_tag="VFR25_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9940075.1"
                     /translation="MRFMMLMIPKGYETAPPDVKLDAERVKAMMKYNESLQKAGVLLA
                     LDGLHPPSSGARVSFAGGKPKVVDGPFPEAKEVVGGYWMIQVRSKEEAVAWASRCPAS
                     DDETIEVRRVQELADFSGDVREAIAGLPEIQAGVGQKR"
     gene            complement(5764..6195)
                     /locus_tag="VFR25_03150"
     CDS             complement(5764..6195)
                     /locus_tag="VFR25_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9940076.1"
                     /translation="MRVMVLVKADKQSEAGALPSGELLEAMGKFNEKLIQAGVMLAGE
                     GLRPSSKGARVRFSGGKKTVIDGPFAETKELVAGFWLWKVDSMDEAIEWLKRAPFEGT
                     EVEIRQVFEAEDFGAEFTPEMREREERHRARAEELSKAGKR"
     gene            6342..7421
                     /locus_tag="VFR25_03155"
     CDS             6342..7421
                     /locus_tag="VFR25_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET9940077.1"
                     /translation="MSRHPTPKRSGTLAAVLVATMVAMSPSPARAAKTDVLTLGNGDR
                     ITGEIKWLTRGKLQYSTDDAGTISIEWVKVARLTSPSSFEVETTTGAKHIGRLVAVDR
                     NGLIVVGGDVIDTLSITNVVAISSLDASFLKRVRSYLDVGLTYAKANEATTFTTEGEA
                     AYRGDKIGTTFGFSSYAQGQESVPTTTRNSVSLRGIRFLPKRWSVAALGMTEQNDELN
                     LELRVSGAAVLGRVLRRSNSSELGAGAGLAVTRERFAPGPGDPPDERETGTNLEGLIF
                     AGWDAFRFDSPKLDFGTSLYLYPSLSTAGRVRGEATLRLKYELISDFNVGVSATDTFD
                     SKPPEETATKNDFITSFTIGWSYRR"
     gene            7473..8111
                     /locus_tag="VFR25_03160"
     CDS             7473..8111
                     /locus_tag="VFR25_03160"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET9940078.1"
                     /translation="MTEGTSRIDAFSDGVFAIAVTLLILEIRLPHAGVEGGLWNGLLA
                     LWPSYFAFVLSFFVILVTWITHHDLMRLVRATNRPVQLANGCALLYVTFIPFPTAVLA
                     ANLTGVDIRTAVAFYCGTFVFGSGAFNVLVATIARGQLFRPEVNAQAIGRIRRGYRIT
                     FAIYLAATLIALVAPWLALAINVAVRLHLLRIRYQPATPAPRPEAELTRERG"
     gene            complement(8092..9612)
                     /locus_tag="VFR25_03165"
     CDS             complement(8092..9612)
                     /locus_tag="VFR25_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET9940079.1"
                     /translation="MLARVLSSTTIGIEGVFVAVEADLGTGLPTFTIVGLPDAAVRES
                     RERVLAAIRNSGFEFPAKKITINLAPAHVRKEGGRFDLPIAVAVLLASGQIPRGIEDG
                     VFLGELALDGGVRGVRGTLAVLAAARREGRGPVWLPSANAAEARAVPGVAIRPIASLR
                     SLRGDEDDAVDAAPTETSWPAEPELDLADVRGQVAPKRALEIAAAGGHNLLMVGPPGA
                     GKTMLGRRLPGLLPPLADEDAIQVSTIYSVAGRLPPGAGLLRRPPFRAPHHSVSSAGL
                     LGTVRGPGELSLAHRGVLFLDELPEFRRDAIEALRQPLEEGHVTITRAGGSVSYPAQV
                     MVVGAMNPCPCGRKGDALRACTCAPGKPEEYVGKLSGALLDRIDLIVDVGAVPADDLF
                     QGARGEPTAAVARRVLAARGADSRRKRGARNADLRNLDLELAAPLDRECRRFLEGAAT
                     ALGISARRILRARRVARTIADLAGSDAVRVDHLAEALQFRLELPAVAGKRATRAPG"
BASE COUNT         1483 a         3371 c         3277 g         1606 t
ORIGIN      
        1 gcggacaagc gtggggaccg ccacgatttg gtcggtgcgc gcgaggcgcg gattctgcgc
       61 caggtcgatg acctcgatcg tgtacttccc tcccagatgt ccctcgcaga tcgacttcag
      121 attctcgagc gcggcgatgg accgtggggt ctggcccgcc acgtagagct tgagaaccca
      181 ctcgtcctcc ggtgcgtcgg tgtcgtgttc ggctcgtgcg tcgtgaggcg tcatggcact
      241 ccgttccctc ccgggccctc cgagggcccg tcctcaccgc gtctcgctcc catcgcctgt
      301 cggcccagag cgagctcgcg aagccgcgac gactcctgat cgatgcgaat ggtgatctcg
      361 ttctgctcgg ccgcgtactg cgcccggagc gcttcgatct gcgcgtcgag cgccagccgc
      421 ttggcctcga ggtcgcggcg catgcgctcg agcgactgct gtcggacgac ggcctcggcg
      481 cgctcctccg cctcgcgcga ggcacgcgcc gagcccgtga gcaccccctc ggggccggtg
      541 tagacgtcct ggaggtgaac accccgctcc gtgatgagaa attcccggat ttggttcgaa
      601 tgcgccgtgc cacgcgactt cagaacgtag aggccccggt tccgctcgcc cgaggactcg
      661 atgtcgcgga gcaggatcca cgtgtcgatc aacgaggaga tccccacatc cgtcgtttcg
      721 gctccgcgcg cggcgagatc ggcgagcacc acggtgatgc tgcgctcctt caggaaatcg
      781 atcaggcgga tgaacatgga cttcgtgtcc gcgtcgttgc tcacgtcgtt gagattgctg
      841 atcggatcga agatcaccat ccggggggcg aactcctcga ccctctgatg cgtgatcgcg
      901 aggtgcgttt cgagcccgta catggtcggg cgggcgctca cgatccggag cagatcctgc
      961 tcgatgaacg gaccgatctc gagccccacg gaccgcatgt tgcgcacgat ttgcgccggc
     1021 gactcctcaa aggagaagta gagggcgcgc tcgcctcgga cacagctcgc gtgggccagg
     1081 tgggtcagaa gggtggtctt gcccgtgccc gcgctgcccg tgacgagaat gctcgatccc
     1141 cgatgcgggc cgccctggag catgtcgtcg agacgaggga tgccggtctg gatgcgctcg
     1201 gacgagacct tgtgggcgag ccccgccgag gtgatgggca ggacgtggat tccatgctcg
     1261 tcgatgtaga acgggaactc gttggtcgca tgcttcgagc cccggtattt gacgacccgg
     1321 agcatgcgcg tgaggatctg ctggcgcacc cgatggtcga ggaggatcac gcagtcggag
     1381 acgtactcct cgaggccgtt ccgggtgagt ggcccatcac cccgctcggc cgtgacgatt
     1441 gcggtcacct cgcggtcctt cagccactgg aagagccggc ggagctcggc ccgaacgacg
     1501 ccaagattct tgaatgaagt gaagaggacc tcgagcgtgt cgagcgagac ccgtttcgcc
     1561 ccgaccgagt cgatcgcgtc gcccagcctg aggaagagac cttcgagatc gtattccccg
     1621 ttctcctcga tctcgaaggg atcgacctgg acgtagtcga cgacgagctt gccctcttcg
     1681 acgagcgcgt ccaggtcgta gccgagagag cggacgttgc gaaccaggtc ctcggcgcgc
     1741 tcctccagcg cgaggatcac cccgggctcg ccgtagcgaa ggatgccgcg cacgacgaac
     1801 tcgatcccga ggagcgtctt gccgcagccg gggccgcccg cgaccagcgt gggacgcccg
     1861 cgcggcaggc cgccgttcgt gatctcgtcg agaccgtcga tcccggtggg gcacttctcc
     1921 aggccgagga gcgccgcgtt gggagattga aacgtcgtgg ccatccagga tgcctctcga
     1981 gcggccctgt gcgggccgct ttcggttcat gggaccaatg tcagagtgat gcgggaccta
     2041 gaagcgtaac gatacgcggg cggggacgac aagccggaaa tgacgggcag gggcggcaag
     2101 tcagataagg ggggtgtctg cggggctagg cgggcaggca ggctccggtc ttcggaacca
     2161 gccggggtaa ggcagacccc ggggtccgcg tctcaagatg gaacgcggaa cccggggcaa
     2221 ccagggaagc ctcctcagcc gacgtattgg cctgcgagct ggcgctcctc gtcgtatttg
     2281 tccgatccgt ggagcttcac gatcttgttg tagacgccga gcatcaagaa ggtcgggccc
     2341 aggctggaga tgaagttctc ccccgagccg ccgccgcgcc ggctccggat cgacctcacg
     2401 gcggaaaccc cgagaaagcc gagggccgcc cacaggaata ggtccgacgg gatcttggag
     2461 gtctggtgct cgatggcctt cgccaccggc ccttcggtgc gtggctgggt gctggattcg
     2521 atcatgggtg ccccgctttc tggcgttggg atcggtcgat gcggcggcga gccgtggggg
     2581 acatcgcgtc ccccggtcgg ctgccgatct tcgtgactcc gcttcgtctc gagaagcgag
     2641 aggttgtcgt tcttcttact tcttcttgtt gctctggcgc gtgccggggg ggttcccggt
     2701 gcgggaattc tcccggtcgt tcgatccggc gttgcgcgag cttcccgtgc gccccgacgt
     2761 cgacgaggtg ccacgatcgt cctcctggac gtcgtcgtca cgatcgtcgt cgcgtttgtt
     2821 atccctgtct cggtccatac gatcctccgt gaatcgtgct ggtgcgtggg ttctcggccc
     2881 ttcggccgac acgcgaggac aggtggcagg ttccatgcca tggaggacat cgacaaggaa
     2941 gacgttgctg ctgcgtcgtg tttcgacagc gcgcgatggg acgcggtggt accgcttcga
     3001 acgatcttgt atccgctaca cgaaggatct acatgcctcg ccctgacctg agcgcggccc
     3061 gctccatgca cctcacccgg ctcgcggtcc acgccctcga gacggtcgac gggcgcgagc
     3121 gggagctcga ccgcgaggag cggcgggacg agcggcagcg gggacgtccg ccggcgccgc
     3181 ctttcgggca cgctccgcga gacggtccgc gcccatccgc ccggccatag cttcgaaacc
     3241 ggcggtcggt ccgcgccatt cgagatcctc gacgtccccg aagagcggcg catccgtgcg
     3301 cagcgtggcg agatccttga agaggagcgc ccgcttccga tcggcggcgc cgagcgcctc
     3361 ctcagggaag ctctcgatcg ggccgtagcg cgcgatcaag cgcgccgccc ccacggcgcc
     3421 gattcccttg atccccggat agccgtccgc ggaatccccc acgagcgcga ggaagtcggg
     3481 aatgcggacc ggctcgacgc cgaacgcctc cttcacgccc gcttcgtcgc gcatcctgtc
     3541 ggcgcggcgg tccatctgca cgactcgatc cccgcgcacg cactgggcga gatccttgtc
     3601 gggcgaccag atgcaaacct tctccacgcg cttgtcgcgc tccgcgatgg cggcggccga
     3661 agcgagacca tcatccgctt cgagctcgat catgggccac acggcgacgc ccatggcaag
     3721 gagcgcgtcc tcgagcgggt ggaactgcgc ccagagcgcg cggtcgatgc cctccccggt
     3781 cttgtatccg tcccagagat cgttccggaa cgactcgatc acatgatcgg tggcgacgcc
     3841 gacgtgcgtc gcccccttct cgatcatctg gagcacggtg ccgagcacgc cggtcacgcc
     3901 gccgaagggc ttgtcctcgc ccttcgtgaa ccggcgaagg ccatagaagt ggcggaagag
     3961 ctcgtaggtc ccgtcgatga gatggacgat catccggtcg cggacgctcc ttccgccgac
     4021 gcgcaccggg ccgcgcgatc gaggaggagg cgacgctcgc ggtcgtttcg cgtcatcgcc
     4081 gccgcgcgct cgaactcggc tcgcgcctcc tcgcggcggc cgagcttggc caggagatcg
     4141 ccgcggacgc tcggcaggag atggtaggcg cggagggcag gctcttcggc aatggcatcc
     4201 acgtgctcga gccctgccgc gggaccgtgc gccatgccga tcgcgacggc gcgattcagc
     4261 tcgatcacag gggagggcgt gcgctccgcg agccgcccgt acagcgcggc gatccgggcc
     4321 cagtcggtct cgtccgcggc gcgggcccgc gcatggcacg cggcgatcgc cgcctggagc
     4381 gcgtacgctc cctccgctcc gcccagcgtc tccgcgcgct cgagcgcggc gaggccgcgc
     4441 cggatgagga gcggatccca gcgcgagcgg tcctggtcga ggaggaggat cggctcgccg
     4501 gtagggccga cgcgcgccgg cgcacgtgac gcctggatct cgagaagcgc gacgagcccg
     4561 tgcacttcgg cctcctcggg caggagctcg gccaggatgc ggccgagccg gagcgcgtcc
     4621 tcgcagagcg cgggacgcat ccagtcctcc ccggcggtcg ccgaatagcc ttcgttgaag
     4681 acgagataga tgacctcgag caccgagtgg agccgcgctt cgagctcgtc cttgcgcggc
     4741 acctcgaagg gaacgttcgc gtccgcgagc gtgcgtttgg cgcgcacgat ccgctgcgcc
     4801 acggtcgcct cgggaacgag gaacgcgcgc gcgatctcgg gcacggagag cccgcctaag
     4861 agccggagcg tgagcgccac gcgcgcctcg ggggggagaa cggggtggca agcgatgaag
     4921 atgagccgga ggaggtcgtc cccgaccgga tcgtcgagcg acgcgtcgag acgatcgcgg
     4981 tcgccgtccc ggctcgactc gagcgcgcgc tcgatctcgg cgtgcttctg ctcgagccgc
     5041 ttctcgcggc ggagccggtc gatcgccttg tgcttggccg ccgccatgag ccaggccgcc
     5101 ggattgtccg gcacccccgt ctcgggccac cgctcgaggg cggccaggag cgcgtcctgc
     5161 gccaggtctt ccgccagccc gacatcgcgc accacgcgcg tgagcgcgcc gatcagacgg
     5221 ggcgcttcga tcctccagac tgcttcgatc gcgcgatggg tgtcggtggc catggccacc
     5281 gatcacacca tcgaacgggc cctcgcgcaa gggcggcggt cagcgcttct gtccgacccc
     5341 ggcctggatc tcgggaagcc ccgcgatcgc ctcacgcacg tcgccgctga agtccgcgag
     5401 ctcctgcacg cgccggacct cgatggtctc gtcgtcggac gcggggcagc gcgacgccca
     5461 cgccaccgcc tcctccttcg agcgcacctg gatcatccag tagccgccga cgacttcctt
     5521 ggcctccggg aagggaccgt ccacgacctt cggcttccct cccgcgaagg agacccgcgc
     5581 cccgctcgag ggcggatgga ggccgtcgag cgcgaggagg acgccggctt tctggagcga
     5641 ctcgttgtac ttcatcatcg ccttcacccg ctcggcgtcg agcttcacgt cgggcggagc
     5701 ggtctcgtag cccttcggga tcatcagcat catgaaacgc atggctcggc tcctttcggg
     5761 tggtcagcgc ttccccgcct tgctcagctc ctcggcccgc gcgcgatgcc gctcttcccg
     5821 ctcgcgcatc tcgggggtga actcggcgcc gaagtcctcg gcctcgaaca cctgacggat
     5881 ctcgacttcg gtcccctcga agggcgcgcg cttcagccac tcgatcgcct cgtccatcga
     5941 gtccactttc cagagccaga atccagccac gagctccttc gtctcggcga acggcccgtc
     6001 gatcacggtc ttcttgccac ccgagaaccg gacccgcgcc cctttggagc tcggccggag
     6061 gccctctccg gccagcatca cgcccgcctg gatcagcttc tcgttgaact tgcccatggc
     6121 ctccagcaac tcaccgctgg gaagggctcc cgcctccgac tgcttgtccg ccttgaccag
     6181 caccatgacg cgcatcgtat tgcctccctt tcgcttacgt ttgccttgag aatccttcga
     6241 ggctccatcg agccccgctt ctaccctcac gtcgagcggc gatgccccga atcgacatcg
     6301 gcccgcgatt ttcttcggac cgtcgtttat ggtggcgcac tgtgagccgc catccaaccc
     6361 cgaaacggtc cggcacgctg gcggcggtgc tcgtcgcgac gatggtcgcg atgtccccgt
     6421 cgccggcacg cgccgcgaag accgacgtcc tgaccctcgg caacggggat cggatcacgg
     6481 gggagatcaa gtggctcacg cgcgggaagc ttcagtactc gaccgatgac gccgggacga
     6541 tctcgatcga atgggtgaag gtcgcgcgcc tcacgagtcc cagctccttc gaggtggaga
     6601 cgacgaccgg cgccaagcat atcggacggc tcgtggccgt cgaccggaac gggctgatcg
     6661 tggtgggcgg agacgtgatc gacacgctct ccattaccaa cgtggtcgcg atcagctcgc
     6721 tcgacgccag tttcctgaag cgcgtgcgtt cgtatctcga cgtggggctc acctacgcga
     6781 aggccaatga ggcgacgacg ttcacgaccg agggcgaggc cgcctaccgc ggcgacaaga
     6841 tcgggaccac gttcggcttc agctcctacg cgcaggggca ggagtcggtc ccgaccacga
     6901 cgagaaacag cgtctccctg cgcgggattc gcttcctgcc gaaacgctgg tccgtggccg
     6961 ccctgggcat gaccgagcag aacgacgagc tgaatctgga gctgcgcgtg agcggcgcgg
     7021 ccgtgctcgg gcgcgtcctc aggcgatcga actcatccga gcttggagcg ggagccgggc
     7081 tcgccgtcac gcgtgagcgc ttcgcgcccg gcccaggcga cccgccggac gagagggaga
     7141 cagggaccaa tctggagggc ctcatcttcg ccggctggga cgcgttccgg ttcgacagcc
     7201 ccaagctgga tttcgggacg tcgctctacc tctaccccag cctctccacc gccgggcgcg
     7261 ttcgcggcga agccacgctc cgcctcaagt acgagctcat ctccgatttc aatgtcggcg
     7321 tgagcgccac cgacacgttc gacagcaaac cccccgagga aaccgcgacc aagaacgact
     7381 tcatcacgtc gttcacgatc ggctggtcct atcggcggta ggcgaagggc ccggtcccgg
     7441 gcatgggtgt tggagctgga cgggaggttg cattgaccga aggcacgagc cgcatcgacg
     7501 cattcagcga cggcgtcttt gccatcgcgg ttaccttgct gattctcgag atccgccttc
     7561 cgcacgcggg ggtcgaaggt ggactgtgga acggtctgct ggcactctgg ccctcctact
     7621 tcgcgtttgt gctcagtttc ttcgtcattc tggttacgtg gatcacccac cacgacctca
     7681 tgcggttggt ccgtgcgacc aatcgcccgg tacagctcgc gaacggctgc gcactcctct
     7741 atgtgacctt catcccgttc ccgaccgcgg ttctcgcggc caaccttacg ggggtggata
     7801 tacgtacggc cgttgctttc tattgcggca cgtttgtctt tggcagcggg gcgttcaacg
     7861 tgctggtcgc gacgatcgcg cgcggccaat tgttccgccc ggaggtcaac gctcaggcca
     7921 tcggccgcat ccgccgaggt tatcggatca cgtttgcgat ctatctcgcc gcgacgttga
     7981 tcgcgctggt agcgccgtgg ctggcgctcg cgatcaacgt ggcggtgcgg ctgcatttgc
     8041 tccgcatccg gtatcagccg gcaacgcccg cgcccaggcc agaggcagaa ctcacccggg
     8101 agcgcgggta gcgcgctttc ccgcgaccgc tggaagctcc aggcgaaact ggagcgcctc
     8161 ggcaaggtga tccacccgca ccgcatccga tccggcgaga tcggcgatcg tcctcgccac
     8221 ccggcgggcg cggaggatcc gccgcgccga gatgccgagc gcggtggcgg cgccctcgag
     8281 gaaccggcgg cactcccggt cgagcggcgc cgcgagctcg agatcgagat tccggagatc
     8341 ggcgttgcgc gctccacgct tccggcgtga atccgcgccg cgcgcggcga gaacccgccg
     8401 tgccaccgcg gccgtcggct cgccccgcgc cccttggaag agatcgtcgg ccggcaccgc
     8461 gcccacgtcc acgatgagat cgatccggtc gagaagcgca cccgagagct ttcccacgta
     8521 ctcctccggt ttgcccggcg cgcacgtgca ggcgcggagc gcgtccccct tgcgcccgca
     8581 cggacacgga ttcatcgctc cgaccaccat cacctgcgcc ggataggaga ccgatccgcc
     8641 cgcccgcgtg atcgtcacat gtccctcctc gagcggctgc cggagggcct cgatcgcgtc
     8701 ccgccggaat tccgggagct cgtcgaggaa gaggacgccc cggtgggcca ggctgagctc
     8761 gccggggccg cgcacggtgc cgaggagccc ggcgctcgac accgagtgat gcggcgcacg
     8821 gaatgggggc cggcggagga gtccggctcc cggggggagc cgtcccgcca ccgagtagat
     8881 cgtgctcacc tggatcgcat cctcgtcggc gaggggcggc aggagtcccg gcaggcgtcg
     8941 cccgagcatg gtcttgccgg cgccgggcgg acccaccatg aggaggttgt gccctccggc
     9001 ggccgcgatt tcgagcgcgc gcttgggggc gacctgcccc ctgacgtcgg ccagatcgag
     9061 ctccggctcc gcgggccatg acgtctcggt cggtgcagcg tccaccgcat cgtcctcgtc
     9121 cccacggagc gagcgaaggg acgcgatggg gcggatggcc acccccggca cggcccgcgc
     9181 ctccgccgcg ttcgcgctgg gaagccacac gggcccgcgc ccctcccggc gcgcggccgc
     9241 gagcaccgcg agcgttcccc gcacgccgcg caccccgccg tcgagtgcga gctcccccag
     9301 gaacaccccg tcttcgatcc cgcgcgggat ctggcccgag gcgaggagca cggccaccgc
     9361 gatcggaaga tcgaagcgcc cgccctcctt ccggacgtgc gccggtgcga gattgatcgt
     9421 gatcttcttc gccggaaatt cgaaccctga gttccggatt gcggcgagga cccgctcgcg
     9481 gctctcccgg accgcggcgt cggggagccc gacgatggtg aaggttggaa gcccggttcc
     9541 gaggtctgct tcgaccgcca cgaagacgcc ctcgattccg atcgtggtgg agctcaagac
     9601 gcgtgcgagc acgggaggtc cctgcttcgg ggtatcatgc gggagagggg cggagcttcg
     9661 aggcacccaa acggtacgag gcgccgcccc ctctgtcaat cgcgcggcgg aggcgcatgg
     9721 ccgaatcgat cggaatc
//
LOCUS       DASTKC010000071        15862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1233894, whole genome shotgun sequence.
ACCESSION   DASTKC010000071 DASTKC010000000
VERSION     DASTKC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15862
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233894"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VFR25_03170"
     CDS             <1..1296
                     /locus_tag="VFR25_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9940080.1"
                     /translation="IIGSGIFRSPSSVARELPSQPLMLAAWILGGILSMAGSLALAEL
                     AVAHPKTGGLYVFIRESFGPRLGFVFGWASLAVIKPTVIASIASVFAIYFCQVVGWSK
                     GAELPVGLTAIGVLTFVNWLGVREGTATQTTLTTLKVAGILLLCFAAFALPHHAPDAA
                     ASTGAPSNPAPANPMHPLWLAFVAAMIPIFFAYDGWTDSTYVAGEVVNPRRNLPIAIL
                     GGTLVVIAVYVLTNLAYFAVLTPTEMAQATAVGSETVRRILGEWGGRALAVLVAVSTF
                     GTISASILTGPRVTLAMASDGLFWKPAAHVHERRGSPDIALWLQCVLSWVWLTVAVGG
                     FEDVSGWFVTTSWLFYGLTVAGVFVLRRKESAAGREPEASYRTWLYPITPVLFILVTL
                     AIIGSDLASSTWRAAAGVVIAALGLPIYHFWFGRRERGA"
     gene            complement(1297..2529)
                     /locus_tag="VFR25_03175"
     CDS             complement(1297..2529)
                     /locus_tag="VFR25_03175"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9940081.1"
                     /translation="MLSAPAPVSLLALAQFLSQFGDSVFQIAFLWLLLDLTGSKSVTG
                     LAATVSYLPPLLFGLVAGVLVDRWDRRRVLIGADLARATLLGLLALLWGLRILTAPWL
                     TAVAFGMATAAVLFNPSRDSLLPEIVSAGALTRANAWVQSSQQFAFLLGPLTASVIID
                     RATIGATFPAGIALFLGSMGLLFAMRVPPRVDAARASAELVGSRNLTGAFRQVFRELL
                     AGLRELGRIPPLPFLYALTALDNLFIMGVSVLGLVVLVRETLGGTAADYALAEATYGV
                     GMVAGSLAVVRWGNRWSSGALLLIGITLDGLTYLPLLACRSMATLVPALLVHSFAIPL
                     ITVPRATILQRLVPPRLVGRAFALLNVVVLGVTALSCGLAGMALERVSAPVLFALAGA
                     LGGATGLVGFLSLRLRSL"
     gene            complement(2549..3076)
                     /locus_tag="VFR25_03180"
     CDS             complement(2549..3076)
                     /locus_tag="VFR25_03180"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9940082.1"
                     /translation="MQNKALMAGFWDHFRTVNGITLRCIEAIPADKIDAHPCQEMRTV
                     KELVTHMYAQMRYVAEGIPKGHTPYNGEQDKDAMAGIKTRDDLLRYAKESWTIADRTV
                     KSLTDAQLQAIVKTEWGGMSFPGWIIVSIIYDEHLHHRGQLYTYLRQLGIEPPFLWDF
                     ENNAPEFRPAQTQQA"
     gene            complement(3100..3840)
                     /locus_tag="VFR25_03185"
     CDS             complement(3100..3840)
                     /locus_tag="VFR25_03185"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET9940083.1"
                     /translation="MKLRILLLAAFLVTVSAAGVYAVTKEFTPDWSFNATIIEACSCP
                     MFCQCYFDHKPASHADHEGMAGMSHGGEHAYCKFNNAFRVNKGNYGGVKLDGAKFWVA
                     GDLGPEFADGEMDWAVLHFDPSVTPEQREGIKNALGYVYPVKWKSFTVGADAPMEWTA
                     TKDVATAKLDGGKVGEVVLKRNQGMTDQPIVIQNLRYFGVPRNTGFVLMQNEVEAYRA
                     GDKPYEYKGTNGFMITLDINSKDVKPKS"
     gene            complement(4021..4755)
                     /locus_tag="VFR25_03190"
     CDS             complement(4021..4755)
                     /locus_tag="VFR25_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940084.1"
                     /translation="MLRVRFLILALLAVARMGVAAPTDETPTYATLPATDTERTSAQR
                     SLVAAVSSLPAAASGFRLAPDGASEEVGRVVLKLDGGKQPWGPLDASAERIYNRTDAS
                     DDEDRTLEVRVSVNGTKGLSTGLASVGGTPRPFTVAGAFALEQNLIGAEDSGGRVAMP
                     VDPSQVERTVALLRIYIVPKALEERLRLMAKGSEDFPAFDSSFVPSSKRAATRSLVVE
                     IHGQKRDVEAYARRVHAADLRRRIEG"
     gene            complement(4814..5917)
                     /locus_tag="VFR25_03195"
     CDS             complement(4814..5917)
                     /locus_tag="VFR25_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9940085.1"
                     /translation="MLDEKRRAGRPVVDLAETNPTRVGLPSIAGALGGDVARYDPDPR
                     GSAAAREAIAAYSRARGAGSFDPDRILLTASTSEAYAHLFRLLCDPGDEILVPSPSYP
                     LFEPLARLESARLASYRLRYDEGWRVDVDSVEALVGPRTRALIVVQPNNPTGSCLTEG
                     EAAALESICAERRIALIADEVFGDFGWREAVLPSVVGRGRALRFGLNGISKLCGLPQM
                     KLGWIAVTGPEDAVARACQGLEWIADLFLSVGAPAQMMLGRWLERRGEFQEAARARIG
                     ANLDQLRLAGAAAGFDVLRGDGGWSAILRFRMGTAAGDASDAAAWALEERDVLLHPGH
                     FYELEDCDAVVSLIVEPRHVREGIERIVGMRSG"
     gene            complement(5962..6312)
                     /locus_tag="VFR25_03200"
     CDS             complement(5962..6312)
                     /locus_tag="VFR25_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9940086.1"
                     /translation="MAKALLHFVVVVAMFLVLTKIIPGFYVPNWGTAILAALLFGVLN
                     STLGLILKILTFPLILVTFGLFYFVVNAVILLLVAWIVPAFSINGFWPALLAAVVLAA
                     VNTVWKMATAEAKD"
     gene            6836..7519
                     /locus_tag="VFR25_03205"
     CDS             6836..7519
                     /locus_tag="VFR25_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9940087.1"
                     /translation="MELRDRLARIRDRMAEAARRSGREPDAVTLLAVTKGFGVEAAQS
                     AFALDVVDLGENRVQEAEGKIAAMGKGPRWHLIGHLQTNKAKRAAQLFDEIHSVDSLR
                     LAEELARRATAEGRTIRCYIEVRTTGESTKSGAAPEEIHALVERMRSLAPLRVAGLMT
                     MGPLHGGPDGARSSFRALRRLRDEGIDRGVLDADAGLSMGMSDDFEVAIEEGATIVRI
                     GSALFGPRP"
     gene            7516..7782
                     /locus_tag="VFR25_03210"
     CDS             7516..7782
                     /locus_tag="VFR25_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET9940088.1"
                     /translation="MRFRIQVTPKSRADTVEADPATRMIRVRVTAAPESGRANEAVVA
                     ALRKKLGLNAGALRIVGGLAERRKWVEVDGIEEAELWRRLEAGS"
     gene            7755..8642
                     /locus_tag="VFR25_03215"
     CDS             7755..8642
                     /locus_tag="VFR25_03215"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET9940089.1"
                     /translation="MAETRGGVVTVDGARATGLLRDIEEARAFVAARAKLKPEVGIVL
                     GTGLGQFAAAMRDPVAIPYADIPHFPHTKVESHAGDLIVGSLAGRPTAILSGRAHYYE
                     GWSMQEVTFPVRVLRALGVDTLVVTNAAGGMNPLYEAADIVAVVDHINLMGDNPLIGP
                     NEDSLGPRFPDMSEPYDRALLKAAREVALAEGIPLKEGVLAAVAGPNLETRAEYRFLR
                     WAGADLVSMSLVPEAIVAAHAGMRLLAFAVVTDLCLPDALEAVDIGRILANAAKAEPK
                     LTRLVTRVLAELPPRASRA"
     gene            8639..11815
                     /gene="ileS"
                     /locus_tag="VFR25_03220"
     CDS             8639..11815
                     /gene="ileS"
                     /locus_tag="VFR25_03220"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET9940090.1"
                     /translation="MSVKADRFRPFETTLDSPASERKILEFWKEVRAFEEGLKRREGS
                     PPFLFYEGPPTANGLPGVHHVLSRTVKDVVCRYQAMRGRYVARKGGWDTHGLPVEIEV
                     EKALGISGKDQIEQFGVAEFNARCRESVLKYENEWRRNTERIGFWLDMDHPYLTFSND
                     YIESVWWLLKRFWDSGLLYLGHKIVPYCPRCGTPLSSHEVSLGYDEVVEPSVTVKFAL
                     VDEPGAYVLAWTTTPWTLPGNVALAVGGDVDYVRVKQTVDGKDERYYLARARLAQLEG
                     EYAIEAEMKGRALVGKRYQPLFDFIDLEEVTGKKAYMVVDADFVTTEDGTGVVHTAVM
                     YGEEDYQLGKKLDLPQHHTVDSRGRFTAEVTPWAGEFVKEAEPEIRRWLRKHGSLYRE
                     EMTTHSYPFCWRCDSPLLYYAWKTWYIATTRYRDQLLAAHKTVEWHPADIGSNRFGNW
                     LENNIDWALSRNRYWGTPLPIWRCASCGSERCVGSVAELKQGKNVPENLDLHRPYVDQ
                     VTFDCPGCGADQRRVPEVIDVWFDSGAMPYAQWHYPFEHEKDFDRLFPADFVSEGMDQ
                     TRGWFYTLMAIAVHVSGRAPYRHVLPLELILDKQGKKMSKSKGNTVNPETILDQRGAD
                     ALRFYLIAVSPLWSTTRFDIEGVTEVTKKLLGTLRNVGQFFALYANIDDYDPARKEPS
                     SPTLLDRWIASRLQTLIGATRDALDAYDLTRGARAIQDFVLEELSNWYVRRSRRRFWK
                     AGDPADKRAAYDTLWECLRVVSRLLAPYTPFVAEELHQNVIRPGSSGEPESVHWCDFP
                     EPDRSRIDERLERSMDLALRVVNLARAARNASSLRTRQPLRRLAVAGLGNQDAQELAG
                     LAGIVLDELNVKELVLNVDREDLVSVTVKPNFPVLGKKAGPAMKEIAARIGETPPETI
                     RAGIAGGGWEVEVGGRSFTLMADDVLVVEASKQPWVGLADGTIAVAVDTTLDEELRTE
                     GLVREFAHRIQALRKSADLDVTDRIRLSWQLSPVLAKACERFEEYIREEVLADELVSR
                     APAGGAVEEWTFDGEQARVGIERVHKGG"
     gene            11818..12207
                     /locus_tag="VFR25_03225"
     CDS             11818..12207
                     /locus_tag="VFR25_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET9940091.1"
                     /translation="MLTKKDLKHFEERLLEERKKLLGQLGYLEKTMSQTQRDAGGDLS
                     AYSFHMADMGTDAMEREKAFLFASAEGKLLYNVDQALRRLYRSEYGTCGSCGKEIGKA
                     RLDAIPHAGLCVECQEKQEKEANATRG"
     gene            12204..12713
                     /gene="lspA"
                     /locus_tag="VFR25_03230"
     CDS             12204..12713
                     /gene="lspA"
                     /locus_tag="VFR25_03230"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9940092.1"
                     /translation="MTQRDGFFYGCAAAVIAADQFTKAIASSRLLPGQPVPFLGDVVR
                     FTLVHNTGAAFGLFPGSRGPFILISILAIIVVLALFRREAYRGLAHRILLGCILGGAI
                     GNLIDRARLGWVVDFIDVGVDTVRWPVFNVADSAVTLGVLFLAWNLSRAGHPAPDPES
                     SHRPGLESG"
     gene            12634..13638
                     /locus_tag="VFR25_03235"
     CDS             12634..13638
                     /locus_tag="VFR25_03235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9940093.1"
                     /translation="MPGTSPERDIPLPIPKVHTVPASRAGERLDRFLAEAERSLSRSA
                     VQQLIDGGHVRLNDAPARASRRVREGDRVEVVRPRREPQALRPEAIPLEVVFEDEHLA
                     VIAKPAGMVVHPAVGHRSGTLVHALLHRYGVLPDGTGSPERAGIVHRLDKGTSGLLLI
                     ARTDEAHRELARQIEAREVKRVYRAVVWGHLAKRTGRIEASLARSPRDRKKIAVVTRG
                     GRHAATRYQVLRQGEYLTELHATLETGRTHQIRVHLAHLGHPVFGDPDYGGRRGPLMK
                     LAPARRAEAALLLADLDHQALHAETLGFTHPATRERLEFTRPVPPEFEAVLDTLPCGS
                     "
     gene            14054..15319
                     /gene="mltG"
                     /locus_tag="VFR25_03240"
     CDS             14054..15319
                     /gene="mltG"
                     /locus_tag="VFR25_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9940094.1"
                     /translation="MRRRSRRLRRTLTLGAIVGVVLLIGLLLVPANVTKNFEQRQVLI
                     PRGAKIEAIADSLHQEGLIGNKKLFVLSARIMGYDRNLKAGQFTIPVGSSTYRILRQL
                     AKGMSAGDMVTIPEGLRVDQIADILHKGAKIDPIRFMALANDSAFMHSIGVPADRVEG
                     YLYPDTYPFYPLLTPQEVMRVMVERSLKVFGEEMALPDAKMGLTLHQLVTLASVVEAE
                     AQVPSERPRIAAVFYNRLHQGMMLQSDPTVLYALGEWRGRVFYKDLDVKSPYNTYRNR
                     GLPPGPICNPGRGAVHAVLFPNADSTELYFVARGDGTHIFTKSWEDHLRAIAMVRAQA
                     RRESTLVPNSAGLVEEAPPPAPTPDAKTSFPVAAKSAQQAPKTTATPPAPKATTSHRS
                     KTSTPSSRKQVPSGKAAFSLKSSVRDSTR"
     gene            15316..>15862
                     /locus_tag="VFR25_03245"
     CDS             15316..>15862
                     /locus_tag="VFR25_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HET9940095.1"
                     /translation="MSPSSEAGLTLRGPGIDPELRAKRDGLRAILETLGRTVVAYSGG
                     VDSAFLLVEARRVLGDRVEGAIADSPSLPRAELDQALALARDRDIPVRVIETREMERE
                     AYVANGPDRCYHCKAELFGCLEAVAEKESWSTIAYGAVTDDLGDVRPGMRAADRYQVR
                     APLLEAGLGKLEVRVLARGLGL"
BASE COUNT         2629 a         5389 c         5359 g         2485 t
ORIGIN      
        1 atcatcgggt cgggaatctt ccggtcgccc agctcggtcg cacgcgagct gccgtcgcag
       61 cccctcatgc tcgccgcgtg gatcctgggc ggcatcctct ccatggccgg ctcgctcgca
      121 ctggccgagc tcgccgtggc gcatccgaag accggcggac tctacgtctt cataagggaa
      181 tcgttcggac cgaggctcgg ctttgtgttc ggctgggcaa gcctcgccgt catcaagccc
      241 accgtcatcg cgtccatcgc gagcgtgttc gccatttact tctgccaggt ggttggatgg
      301 tcgaagggcg ccgagcttcc ggtcggcctc acggccatcg gggtgctcac attcgtgaac
      361 tggctcggtg tgcgggaagg caccgcgacc cagacaacgc tcacgacttt gaaggtcgca
      421 gggatcctcc ttctatgctt cgcagcgttc gcgctgccgc atcacgctcc ggacgcagcc
      481 gcgtccaccg gcgcaccttc caatcccgcg ccggcgaatc ccatgcaccc gctgtggctc
      541 gccttcgtcg cagcgatgat cccgatcttt ttcgcctatg acggctggac ggactccacg
      601 tacgttgctg gcgaggtggt gaatccgcgc cgcaatctgc ccatcgcgat cctgggcggt
      661 acgctcgtgg tgatcgcggt ctacgtgctc acgaacctcg cctacttcgc cgttctcacg
      721 ccgacggaga tggcacaggc cacggcggtg ggcagcgaga ccgtgcggcg gatcctcggc
      781 gagtggggcg gccgcgcgct ggcggtgctg gttgcggtat cgacctttgg cacgatcagc
      841 gcgtcgatcc tcaccggtcc gcgcgtgacg ctcgccatgg cctcggacgg cctcttctgg
      901 aagcccgccg cgcacgtaca tgagcggcgc ggaagcccgg acatcgcgct ctggctccag
      961 tgcgtgctct cgtgggtctg gctcaccgtc gccgtcggcg gcttcgaaga tgtctccggc
     1021 tggttcgtca ccacgtcgtg gctcttctac ggcctgaccg tggccggcgt cttcgtcctg
     1081 cgtcggaagg aatcggcggc cgggcgcgag ccggaagcgt cgtaccgcac ctggctctat
     1141 cccatcacgc ccgtgctctt catcctcgtg acgctcgcga tcatcggcag cgatctcgcc
     1201 tcgagcacct ggcgcgccgc cgcgggtgtc gtgatcgccg cgctgggact gccgatctac
     1261 cacttctggt tcgggcggcg agagcgcgga gcgtagctac aggctccgca accgcagcga
     1321 caggaatcca acgaggccgg tcgcgcctcc gagcgcaccg gccagcgcga agagaaccgg
     1381 cgcggagacg cgctccagcg ccatcccagc cagcccgcac gagagcgcgg tcaccccgag
     1441 caccacgacg ttcaagagcg cgaacgctcg tccgaccagc cgaggaggga cgagcctctg
     1501 caagatggtc gcgcgcggaa cggtgatcag cgggatcgcg aacgaatgga cgaggagtgc
     1561 cgggacgagc gtcgccatcg accggcacgc gaggagaggg agatacgtga ggccgtcgag
     1621 cgtgatgccg atgaggagga gcgctccgct cgaccatcgg ttgccccagc gcacgaccgc
     1681 gaggctgcct gccaccattc cgactccgta ggtcgcttcg gcgagagcgt agtccgcagc
     1741 agtgcctccc agggtctccc gcacgagcac gacgagcccg agcaccgaaa cgcccatgat
     1801 gaatagattg tccagcgcgg tgagcgcata gaggaatgga agcggcggga tgcgtccgag
     1861 ctcccgaagc cccgctaaga gctcgcggaa gacctggcga aaggcgcccg tcaggttcct
     1921 cgagccaacc agctccgcgc tggcccgagc cgcgtccacc ctcggcggca cccgcatcgc
     1981 aaacagcaat cccatcgacc cgaggaacaa ggcgatcccg gccgggaagg tcgcgccgat
     2041 cgtcgcacgg tcgatgatca cgctcgccgt cagcggtccg aggaggaacg cgaactgctg
     2101 cgaggactgg acccaagcgt tcgcccgcgt cagggctccg gcggaaacga tctccggcag
     2161 gagggaatca cgacttggat tgaagaggac cgcggccgtg gccatcccga aggccacagc
     2221 cgtcagccat ggggccgtca agatccggag accccacagg agggcgagca gcccgagcag
     2281 cgtggctcgc gccagatccg cgccgatcag gacccgccgc cgatcccacc ggtccaccag
     2341 gaccccggcg accagaccga acaagagcgg cggcaagtac gacaccgtgg ccgcgagccc
     2401 cgtcacggac ttcgagcccg tgagatcgag caggagccag aggaacgcga tttgaaaaac
     2461 ggaatcgccg aactgggaga ggaactgcgc gagggcgaga agcgaaacgg gggcgggcgc
     2521 cgaaagcacc cgccccctca tcaagccgtc acgcctgctg cgtctgggcg ggacggaatt
     2581 ccggagcgtt gttctcgaaa tcccacagga acggaggctc gatcccgagc tgacgcaggt
     2641 acgtgtacag ctgtccgcgg tggtggaggt gctcatcgta gatgatgctg acgatgatcc
     2701 acccggggaa ggacatcccg ccccactcgg tcttgacgat cgcctggagc tgcgcgtccg
     2761 tcagcgactt cacggtgcgg tccgcgatcg tccacgactc cttggcatag cgcaggagat
     2821 cgtcacgcgt cttgatcccc gccatcgcat ccttgtcctg ctcgccgttg tagggcgtgt
     2881 gtcctttggg gatgccctcg gccacgtagc gcatctgcgc atacatgtgg gtgaccagct
     2941 ccttcaccgt gcgcatctcc tggcacgggt gggcgtcgat cttgtccgca gggatcgcct
     3001 cgatgcaccg cagggtgatg ccgttgaccg tgcggaagtg atcccagaat ccagccatca
     3061 gtgctttgtt ttgcatgaat tctcctgttc gtccggcgct cagctcttgg gcttcacgtc
     3121 cttggagttg atgtccaggg tgatcatgaa tccgttcgtt cccttgtact cgtacggctt
     3181 gtcgcccgcg cggtaagcct cgacctcgtt ctgcatgaga acgaagccgg tgttccgagg
     3241 gacgccgaag taacgaagat tctgaatgac gatcggctga tccgtcatcc cctgattccg
     3301 cttgaggacg acctcgccga ccttcccgcc gtcgagcttc gcggtcgcga cgtccttggt
     3361 ggcggtccac tccatcggag catccgcgcc gacggtgaag gacttccact tgaccggata
     3421 gacgtagccg agagcgttct tgatgccttc acgctgctcc ggcgtcaccg acggatcgaa
     3481 gtggagcacc gcccagtcca tctcgccgtc ggcgaactcg gggccgagat caccggcgac
     3541 ccagaacttc gcgccgtcca gcttgacgcc gccgtagttg cccttgttga cccggaacgc
     3601 gttgttgaac ttgcagtagg cgtgctcgcc gccgtggctc atgcccgcca tgccctcgtg
     3661 atccgcatgg ctggccggct tgtggtcgaa gtagcactgg cagaacatcg ggcaggagca
     3721 tgcctcgatg atcgtcgcgt tgaagctcca gtccggcgtg aactccttgg tgaccgcgta
     3781 cacgccggcg gcgctcaccg tgacgaggaa tgcagcgagc aagagaatgc gcagcttcat
     3841 gggaggtcct ccttgaggga tgtggggagt gacgctccgg aatccggaac ggtgagcccc
     3901 cgttccgccc ggatcagcag gcgttaggtg tacacctttc ggagccgttc gtgaagagtg
     3961 ggcttttggg cgccaccatc ggggcctgac ccggcccgga cccgaagcgg cccgcgcccc
     4021 tcagccctcg atccgccgcc tcagatccgc ggcgtgcacc cgccgcgcgt aggcctcgac
     4081 gtcccgcttc tgtccgtgaa tttcgacgac gagggagcgc gtggccgccc gcttggagga
     4141 aggcacgaag ctggagtcga aggccggaaa gtcctcggag cccttcgcca tcaagcgcag
     4201 ccgctcttcc agggccttgg gaacgatgta gatccggagg agggccaccg tccgctcgac
     4261 ctgggagggg tccacgggca tcgccaccct ccccccggaa tcttcggcac cgatcaggtt
     4321 ttgctccagc gcgaaggctc ccgccacggt gaagggccgc ggcgttcctc ccacggaggc
     4381 cagccccgtg gacaggccct tggttccatt cacggacacc cggacctcca gagtccggtc
     4441 ctcgtcgtcc gaagcatcgg tccggttgta gatccgctcc gccgatgcgt cgagcggacc
     4501 ccatggctgc ttcccaccat cgagcttgag gacgacgcgt ccgacctcct cggaggctcc
     4561 atccggcgcg agcctgaagc cgctcgcggc tgcgggcagc gacgacacgg cggctacgag
     4621 cgaacgctgg gccgaagtcc gttccgtatc cgtggcgggt agggttgcgt aggtgggtgt
     4681 ctcatcggtc ggtgcggcca cacccatgcg ggccacggcc agcagggcca gtatcaggaa
     4741 acggacacga agcatgcgtg aatcctcccg tcgacgcgga gcgctggcga gaccccgccc
     4801 actcttacac cgttcacccg cttctcattc caacgatccg ctcgattccc tcacgcacgt
     4861 gccgcggttc cacgatgagg ctcaccacgg cgtcgcaatc ttccagctcg tagaagtggc
     4921 ccggatggag gagcacatcg cgctcctcga gcgcccatgc ggcggcatcc gaagcgtctc
     4981 ccgccgccgt gcccattcga aagcgcagga tggcgctcca gcctccgtcc ccacgaagca
     5041 cgtcgaaccc cgccgcggcg ccggccaggc gcagctggtc cagattggcc ccgatccgcg
     5101 cgcgcgccgc ctcctggaat tcgccacgac gctcgagcca tcgaccgagc atcatctgcg
     5161 cgggagcgcc gacggaaagg aacagatcgg cgatccactc gagtccctgg catgcgcgcg
     5221 cgacagcgtc ctcgggcccc gtgaccgcga tccagcccag cttcatctgg gggagtccgc
     5281 acagcttcga gatcccgttc agaccgaacc tgagcgcgcg tccacgaccc accaccgaag
     5341 ggagcacggc ctcgcgccat ccgaaatcgc cgaacacctc gtccgcgatc agtgcgatcc
     5401 gccgctcggc gcagatcgat tcgagagcgg ccgcctctcc ctcggtcaag caggatccgg
     5461 tcggattgtt cggttgaacc acgatcagcg cccgggtcct tggccccacg agcgcctcca
     5521 cggagtccac gtccacgcgc catccctcgt cgtagcgcaa gcggtacgac gcgagccgcg
     5581 cgctctcgag acgcgcgagc ggctcgaaga ggggatagct cggactcggc acgaggatct
     5641 cgtctcccgg atcgcagagg agacggaaga gatgcgcata ggcctcgctc gtgctcgcgg
     5701 tgaggaggat ccgatccgga tcgaacgagc ccgcgccgcg cgccctcgag taagccgcga
     5761 tcgcctcccg cgcggcggct gagccgcgcg ggtcgggatc gtagcgggct acgtccccgc
     5821 ccagcgcccc agcgatcgac ggaaggccca cacgcgtggg gttcgtctcc gcgaggtcga
     5881 cgaccggccg tcccgcgcga cgcttctcgt cgagcaaccg atgaaacgga ttggcctcga
     5941 aatctcccgg gagtcgcgag gtcagtcctt cgcctccgcg gtcgccatct tccagacggt
     6001 gttcaccgcg gcgagcacga cggccgcgag gagcgcgggc cagaagccat tgatgctgaa
     6061 cgccgggacg atccaagcga ccaggagcag aatcaccgcg ttcaccacga agtagaagag
     6121 accgaacgtg acgaggatga gcgggaaggt gaggatcttg agaatgagcc cgagcgtcga
     6181 gttcaggacg ccgaagagga gcgccgcgag gatcgcggtg ccccagttgg gcacgtagaa
     6241 gcccgggatg atcttcgtga gcaccaggaa catcgcgacg acgaccacga aatgaaggag
     6301 cgccttggcc atcgatccct ccttgacgtt gaattcccag gatcccgacg acgccagcgt
     6361 agcagaccca agccgggctt gcgcctacag ctccatggcg ttagtctggc cgcgctccgc
     6421 acgagccggc gcgctcgccc cgcgtcgtga ccgcatcgat ccgccgcatc cgttcccgca
     6481 ggggggctcg gccgctacgg agagcacccc atggacctcg atcattggta ccgctgcacg
     6541 cagtgcggct acatgtacac gccccagcag atcctggcgc tccgtcagct ggtggaggct
     6601 cgcgacgagt ccgagtcccc ttcggccgaa cttccatgcc ggaagcgtgg ctgcggcggc
     6661 cgactcgtgg tgaccacgga agttcaccga gacgcgccac gctgactaga acgccctgat
     6721 ccggcgacct tccgtcgctc cacgggccgc atggccccaa acctgcttgt cgctcgcgag
     6781 ccggactcct atactgaccg gctcgcattt ttttggaggg ccaacgggcg ggagcgtgga
     6841 acttcgagat cggctggccc ggatccggga ccgcatggcc gaggctgccc gccggtcggg
     6901 gcgggagccg gatgcggtga cgctccttgc ggtgacgaag ggattcgggg tggaggccgc
     6961 ccagagcgcg ttcgcgctcg acgtcgtgga tctcggtgag aaccgggtcc aggaggcgga
     7021 gggcaagatc gccgcgatgg gcaagggtcc aaggtggcat ctcatcgggc acctccagac
     7081 gaacaaggcg aagcgcgcgg cccagctctt cgacgagatc cattccgtgg attcgctccg
     7141 gctggccgag gaactcgccc gccgtgcgac cgcggagggc cggacgatcc ggtgctacat
     7201 cgaggtccgg accaccggcg agtcgacgaa gagcggggca gcaccggaag agatccacgc
     7261 gctggtcgag aggatgcgat ccctggcgcc gcttagggtg gcggggctca tgaccatggg
     7321 cccgctccac ggcggtcccg acggggcgcg gtcctcgttc cgggcgctca ggcgccttcg
     7381 ggacgaggga atcgaccgag gcgtcctgga cgccgacgcg ggactctcga tggggatgtc
     7441 ggacgacttc gaagtcgcga tcgaggaggg agcgaccatc gtgaggatcg gctcggccct
     7501 gttcgggccg cggccgtgag gttccggatc caggtcacgc cgaagagccg cgccgacacg
     7561 gtcgaggcgg atccggcgac aaggatgatt cgggtgcggg tgacggcggc tcccgaatcg
     7621 ggaagagcca acgaagcggt ggtcgcggcg ttaaggaaga agctcggtct caacgccggc
     7681 gctctccgga tcgtcggcgg tttggccgag cgccggaagt gggtcgaggt ggacggcatc
     7741 gaggaggcgg agctatggcg gagactcgag gcggggtcgt gacggtggat ggcgcgcgcg
     7801 ctacggggct gctccgcgac atcgaggagg cccgcgcctt cgtcgccgcc cgcgcgaagc
     7861 taaagcccga ggtcggcatc gtgctcggca ccggactcgg acagttcgcc gccgccatgc
     7921 gcgacccggt cgcgattcct tacgcggaca tcccgcattt cccgcacacc aaggtcgaga
     7981 gccacgccgg cgatctcatc gtgggctcgc tcgccggccg cccgaccgcg atcctgagcg
     8041 gccgcgcgca ttactacgaa ggctggtcca tgcaggaggt caccttcccg gtgcgcgtgc
     8101 tccgcgcgct cggcgtcgac acgctcgtcg tgacgaacgc ggccgggggc atgaacccgc
     8161 tctacgaagc cgccgacatc gtggcggtcg tcgaccatat caatctgatg ggggacaacc
     8221 cactcatcgg tcccaacgag gactcgctcg ggccgcggtt cccggacatg tcggagccgt
     8281 acgaccgcgc gctcctcaag gccgcgcgcg aggtcgcgct cgccgaaggc atcccgctca
     8341 aggaaggggt gctggccgcg gtggcgggac ccaatctcga gacgcgcgcc gagtaccgct
     8401 tcctccgctg ggccggtgcc gatctcgtca gcatgtcgct cgtgcccgag gcgatcgtgg
     8461 cggcgcacgc cggaatgcgg ctcctcgcgt tcgcggtcgt gaccgacctc tgcctgcccg
     8521 acgcgctcga ggcggtcgat atcgggcgca tcctcgcgaa cgccgcgaag gccgagccca
     8581 agctgacgcg gctcgtcacc cgcgtgctcg ccgagcttcc tccccgggcg agccgcgcat
     8641 gagcgtgaag gccgaccgct tccgtccctt cgagaccacc ctcgactcgc ctgcctccga
     8701 gcggaagatc ctcgagttct ggaaggaggt ccgcgcgttc gaggagggac tcaagcgccg
     8761 cgagggctcg ccgccgttcc tcttctacga gggaccgccg accgcgaacg gactcccggg
     8821 cgtgcaccat gtcctgtcgc gcaccgtgaa ggacgtggtc tgccgctatc aagccatgcg
     8881 cggccgctac gtcgcgcgca aggggggctg ggacacgcac ggcctccccg tcgagatcga
     8941 ggtcgagaag gcgctcggga tcagcggcaa ggatcagatc gagcagttcg gcgtcgccga
     9001 gttcaacgcg cgctgccgcg agagcgtgct caagtacgag aacgagtggc gccgcaacac
     9061 cgagcggatc gggttctggc tcgacatgga ccacccctac ctcacgttct cgaacgatta
     9121 catcgagagt gtgtggtggc tcctgaagcg cttctgggac tcggggctcc tctacctcgg
     9181 gcacaagatc gtgccctact gcccgcgctg cggcacgccg ctctcgagcc acgaggtgag
     9241 cctcggctac gacgaggtcg tcgagccgtc cgtgacggtg aagttcgcgc tcgtcgacga
     9301 gccgggcgcc tatgtgctcg cctggacgac caccccgtgg acgctcccgg ggaacgtcgc
     9361 gctcgcggtc ggcggggacg tcgactacgt gcgcgtgaag caaacggtcg acggaaagga
     9421 cgagcgctac tacctcgccc gcgcgcgcct cgctcagctc gaaggggagt acgcgatcga
     9481 ggcggagatg aaggggcgcg cgctggtcgg caagcgctac cagccgctct tcgacttcat
     9541 cgatctcgag gaagtgacgg ggaagaaggc atacatggtg gtcgacgccg acttcgtgac
     9601 gaccgaggac ggcaccggcg tcgtccacac tgccgtcatg tacggcgagg aggactacca
     9661 gctcggcaag aagctcgatc tcccgcagca ccacacggtg gactcgcggg gccgcttcac
     9721 cgccgaggtc acgccgtggg ccggggaatt cgtgaaggag gccgagcccg agatccggcg
     9781 ctggctccgg aagcacggct ccctctaccg cgaggagatg acgacccaca gctatccctt
     9841 ctgctggcgg tgcgactcgc cgcttctcta ctacgcgtgg aagacctggt acatcgcgac
     9901 gacgcgctac cgcgaccagc tcctggccgc gcacaagacg gtcgagtggc atcccgcgga
     9961 catcgggtcg aaccgattcg gcaactggct ggagaacaac atcgactggg ctctgtcgcg
    10021 gaatcgctac tggggaacgc cgcttccgat ctggcgctgc gcgtcgtgcg gctcggagcg
    10081 ctgcgtgggg agcgtcgcgg agctcaagca gggaaagaac gttcccgaga acctcgatct
    10141 ccaccgtccg tacgtggatc aggtcacctt cgactgcccg gggtgcggcg cggaccagag
    10201 gcgcgtgccc gaggtcatcg acgtctggtt tgactcgggg gccatgccgt acgcgcagtg
    10261 gcactacccg ttcgagcacg agaaggactt cgaccggctc ttcccggccg acttcgtgtc
    10321 ggaggggatg gaccagactc gaggctggtt ctacacgctc atggcgattg cggtgcacgt
    10381 ctcgggacgc gccccctacc gccacgtcct gccgctcgag ctgatcctcg acaagcaggg
    10441 caagaagatg agcaagtcga agggaaacac ggtcaatccc gagaccatcc tcgaccagcg
    10501 cggggccgac gcgctccgct tctatctcat cgcggtgagc ccgctgtggt cgacgacgcg
    10561 gttcgacatc gagggggtga ccgaggtcac gaagaagctc ctcggcacgc tccggaacgt
    10621 ggggcagttc ttcgcgctct acgcgaacat cgacgactac gatcccgccc ggaaggagcc
    10681 ctcgagtccg acgctcctcg accggtggat cgcgtcgcgc ctccagacgc tgatcggggc
    10741 gacgcgcgac gcgctcgacg cgtacgacct gacgcgcggc gcgcgcgcga tccaggactt
    10801 cgtgctcgag gagctctcga actggtacgt tcgccggtcg cgccggcggt tctggaaagc
    10861 cggcgacccg gccgacaagc gtgcggcgta cgacacgcta tgggaatgcc tccgcgtcgt
    10921 gtcgcggctc ctcgcgccct acacgccgtt cgtggccgag gagctccacc agaacgtgat
    10981 ccgccccggg agctccgggg aaccggagag cgtgcactgg tgtgatttcc ccgaaccgga
    11041 ccggagccgg atcgacgagc ggctcgagcg ctcgatggac ctggcgctcc gcgtggtcaa
    11101 cctggcccgc gcggcgcgga acgcttcgtc gctccgcacg cgtcagccgc tccggcgcct
    11161 ggccgtggcc gggctcggca accaggacgc tcaggagctg gcgggcctcg cggggatcgt
    11221 cctggacgag ctcaacgtga aggagctggt cctgaacgtg gaccgtgagg acctcgtctc
    11281 cgtgacggtg aaaccgaact tccccgtgct cggtaagaag gccgggccgg cgatgaagga
    11341 gatcgccgcc cggatcgggg agaccccgcc ggagacgatc cgcgcgggca ttgcgggcgg
    11401 cggctgggag gtggaggtcg gggggcgttc gttcacgctc atggccgacg acgttttggt
    11461 ggtagaggca tccaagcaac cctgggtcgg gctcgccgat gggacgatcg ccgtcgccgt
    11521 cgacaccacc ctggacgagg agctccggac ggaagggctc gtccgcgaat tcgcccaccg
    11581 gatccaggcg ctccgaaaga gcgccgatct ggacgtgacc gatcggatcc ggttgagctg
    11641 gcagctctcg ccggtattgg cgaaagcgtg cgagcggttc gaagagtaca tccgcgagga
    11701 ggtcctcgcg gacgagctcg tgtcccgcgc ccccgccgga ggcgccgtcg aggaatggac
    11761 gttcgacggc gagcaggccc gcgtgggcat cgagcgtgtg cacaaaggag ggtgacgttg
    11821 ctcaccaaga aggatctcaa gcatttcgag gagcggctcc tcgaggagcg gaagaagctc
    11881 ctggggcagc tcggctatct cgagaagacg atgtcgcaga cgcagcgtga cgcgggaggc
    11941 gatctctccg cctactcgtt ccacatggcc gatatgggca cggacgcgat ggagcgcgag
    12001 aaggcgttcc tcttcgcctc ggccgagggc aagctcctct acaacgtgga ccaggcgctc
    12061 cgccggctgt accggagcga gtacggtacg tgcgggtcgt gcgggaagga gatcggcaag
    12121 gcccggctcg acgcgattcc gcacgcaggt ctctgcgtgg aatgccagga gaagcaggag
    12181 aaggaggcga acgcgacgcg cggatgaccc aacgggacgg gttcttctac gggtgcgctg
    12241 cggccgtcat cgcggccgac cagttcacca aggcaatcgc gtcctcgcgg ctcctcccgg
    12301 gacagccggt cccgttcctg ggggacgtcg tccgtttcac gctcgtacac aacacgggag
    12361 cggcgtttgg cctctttccg ggtagccgcg ggccgttcat cctcatctcg atcctcgcca
    12421 tcatcgtggt cctcgcgctc tttcgccgcg aggcctaccg cggcctcgct caccggatcc
    12481 tcctcgggtg tattctcggc ggcgccatcg gcaacctcat cgaccgcgcg cggctcggct
    12541 gggtggtcga tttcatcgac gtgggcgtgg acaccgtccg atggcccgtc ttcaacgtcg
    12601 ccgatagcgc cgtgaccctc ggagtcctgt tccttgcctg gaacctctcc cgagcgggac
    12661 atcccgctcc cgatcccgaa agttcacacc gtcccggcct cgagagcggg tgagcggctg
    12721 gaccgcttcc tcgcggaggc cgagcggtcc ctttcgcgct ccgcggtgca acagctcatc
    12781 gacggcgggc atgtccggct gaacgatgcg ccggcccgcg cgagccgccg ggtgcgcgaa
    12841 ggggatcgag tcgaagtcgt ccggccgcgc cgcgagcccc aggcgctccg tccggaggcg
    12901 atcccgctcg aggtggtctt cgaggacgag catctcgccg tgatcgcgaa gcccgccggg
    12961 atggtggtcc accccgccgt ggggcacagg agcggcacgc tcgtccacgc gctccttcat
    13021 cgctacggtg tgctcccgga cggaactggg agtcccgagc gcgcgggcat cgtgcatcgc
    13081 ctcgacaagg gcacgtcggg actcctcctc atcgcgcgca cggatgaggc gcaccgcgag
    13141 ctcgcgcgcc agatcgaggc gcgcgaggtg aagcgcgtct accgcgcggt cgtctggggc
    13201 cacctcgcga agcggaccgg acgcatcgag gcatcgctcg cccgaagccc acgcgaccgg
    13261 aagaagatcg ctgtggtcac gcgcgggggc cgtcacgccg cgaccaggta ccaagtcctc
    13321 cgccaggggg agtacttgac ggagctccac gccaccctcg agacaggccg cacgcaccag
    13381 atccgcgtcc acctggcgca cctcggtcat cccgtgttcg gagatcccga ctatggggga
    13441 cggcgggggc ccttgatgaa gcttgcgccc gcgcgacgcg ccgaggccgc gcttctcctc
    13501 gcggacctgg accatcaggc gctgcacgcc gagacgctcg ggttcaccca tccggccacg
    13561 cgcgagcggc tcgagttcac gcggcccgtc ccgccggaat tcgaggccgt cctcgacacg
    13621 ctcccgtgcg ggtcttaggg gtggattttg ggctccgaaa cctggggctc gccttgagcg
    13681 atcccggtga ggtgctcgcg acgccgctcc gctcgctccg cctgacttcg gtccggcagg
    13741 ccccggccgc cgtggccgcg gtcgtccggg aagtcgaggc gagcgcggtc gttgtgggag
    13801 cgccagaagg actggagggg gaggagggac gtgccgaatt acggagggtg aaacgcttcg
    13861 ccacagccct tcgccgggag acggggctcc cggtccatct ggtcgacgag agcctgagct
    13921 cgcgggaggc cgaggctaga tcgggccgcg cctccggccg tgaccccgag cgctcttcgg
    13981 ccgcgcacgc ggcctccgcg gccgtcatac tccagcgatg gctcgacaga ccccggaagg
    14041 atgggggcgc ccgatgaggc ggcgctcccg aaggcttcgc cgcacactca cgctcggcgc
    14101 gatcgtgggt gtggtgctct tgatcgggct cctgctcgtg ccggccaacg tgaccaagaa
    14161 cttcgagcag cgccaggtgc tgatcccgcg cggcgccaag atcgaggcga tcgcggacag
    14221 cctccaccag gaagggctga tcgggaacaa gaagctcttc gttctctccg cccgcatcat
    14281 gggatacgac cggaatctca aggcggggca gttcacgatc ccggtcggat cgagcacgta
    14341 ccgcattctc cgccagctcg ccaaggggat gtccgccggg gacatggtca cgatccccga
    14401 ggggctccgg gtcgatcaga tcgccgacat cttgcacaag ggtgcgaaga tcgatccgat
    14461 acgcttcatg gcgctcgcga acgactcggc gttcatgcat tcgatcgggg tgccggcgga
    14521 tcgcgtggaa gggtacctct atcccgacac gtatcccttc tatccgctcc tcacgcccca
    14581 ggaagtgatg cgtgtgatgg tggagcggag tctcaaggtg ttcggcgagg agatggcgct
    14641 ccctgacgcg aagatggggc tcaccctcca tcagctcgtg acgctcgcct cggtggtcga
    14701 ggccgaagcg caggtgccct cggagcgtcc gcggatcgcc gccgtcttct acaaccgatt
    14761 gcaccagggt atgatgctcc agtccgaccc caccgtgctc tatgcgctcg gcgagtggcg
    14821 cgggcgcgtc ttctacaagg atctcgacgt gaaatctccc tacaacacct accgaaaccg
    14881 cgggcttccg cccggtccga tctgcaatcc cggccgtggc gcggtccacg cagtcctctt
    14941 cccgaacgcc gattcgactg agctctactt cgtcgctcga ggcgatggga cccacatctt
    15001 cacgaagtcc tgggaagacc acctgcgcgc gatcgccatg gtgcgggcgc aggcccgccg
    15061 cgagtcgacg ctcgtgccga attcggcggg gctcgtggag gaagcgccgc cgccggcgcc
    15121 cacgccggat gccaagacga gcttccccgt ggccgccaag tccgcgcagc aggcgcccaa
    15181 gaccacggcc acgccgccgg cgccgaaggc cacgacctcc catcggtcca agacctcgac
    15241 gccgtcgtcg cgcaagcagg tgccctccgg aaaggccgcg ttctccctca agtcctccgt
    15301 tcgagacagc acgcggtgag cccctcgagc gaggcagggc tcacactccg cgggccgggt
    15361 atcgaccccg agcttagggc caagcgggac ggcctccggg ccatcctcga gacgctcggg
    15421 cgcaccgtcg tggcctactc tggcggggtc gactccgcgt ttctcctggt cgaagcgagg
    15481 cgtgttctgg gagatcgggt ggagggtgcc atcgcggaca gtccgtcgct tcctcgggcg
    15541 gagctcgacc aggccctcgc cctcgcgcgg gaccgcgaca tccccgtgcg ggtgatcgag
    15601 acccgggaga tggagcggga ggcctacgtg gcgaatggtc ccgaccggtg ctatcactgc
    15661 aaggccgagc tctttggctg cctggaggcc gtcgccgaga aggagagctg gtccacgatc
    15721 gcctacggcg ccgtgaccga cgatctggga gacgtgaggc cggggatgcg cgcggcggac
    15781 cggtaccagg tccgcgcccc tctcctcgag gctggcctcg gaaagctgga ggtccgcgtt
    15841 ctggcccgag gtctgggcct tt
//
LOCUS       DASTKC010000072         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1234204, whole genome shotgun sequence.
ACCESSION   DASTKC010000072 DASTKC010000000
VERSION     DASTKC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234204"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /gene="glgP"
                     /locus_tag="VFR25_03250"
     CDS             <1..2186
                     /gene="glgP"
                     /locus_tag="VFR25_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET9940096.1"
                     /translation="AGPGPVTPFPPMPPELARLPELSRNLWWSWHSEARDLFRSLDET
                     LWRRTRHNPVRLLQEIDPDRLARAAADAGYRVQYIAVMRAFDAALNDEDTWCEEEHPD
                     LADGVLAYFSAEYGVHNSLPLYAGGLGVLAGDITKEASDLGLPFVGVGFMYPQGYFRQ
                     HVNAEGRQEEVYERIERGHVAVSPVRTPDGEPLRVRLSLPDRTLHVAAWQVSIGRSRL
                     FLVDTDLDENTTGDRELTGRLYGGDQNARVSQEILLGIGGVRVLRALGIKPTVWHGNE
                     GHTSLMMLERVREEIAAGLTFDEAVDAVRRTTVFTTHTPVAAGHDAFYYPLMEGHLDP
                     IEGYGVTALGDHRAKLYGLASYPADWGIAFNMTALALRLSGHVNAVSRKHGEVSRAMW
                     APLWPSKKPDEVPIVSITNGVHVPTWIANATDRCLRENLDQDWLRRHDDPALWQKSES
                     IPDEALWALRSHLKLGLFHFLRDRLRQRWAAHDVEASQAVALGALLDPEALTIGFARR
                     FASYKRATLILRDLDRLHHILDDTRRPVQLLFAGKAHPADEGGKDLLQAVYRAARDPV
                     LAGRIAFLEDYDMHAAHWLVQGVDVWLNNPRAPQEASGTSGMKAGINGVPSLSVLDGW
                     WMEAWDGKNGWGFPEAEGDDVDARDVEQLYSLLEKEIVPLYYDRGMDGIPRGWLEVVR
                     HALRTITPAFSARRMVKEYVRTMYAPAMAGKPVLSAPPAPSAPTD"
     gene            2211..2924
                     /locus_tag="VFR25_03255"
     CDS             2211..2924
                     /locus_tag="VFR25_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940097.1"
                     /translation="MPFGVEWHDFVTIGLLVLLEGALSADNALVLAVLVLPLPPEQRT
                     RALRYGILGAFAFRILSTLFATYMIHWDWVKLIGALYLLYLPAKHFIDHPEGKQGGAK
                     AAIKTFLGLSAFWSTVVRVELTDIVFAIDSILVAVAMSRKAWVIVMGGILGILAMRLL
                     IGQLLGIVQRYPAIVDGAYVIVAWVGIKLMIEYFHSIGLIAWEVPRWLSITVVFVLFG
                     ISALYARWKEQKKVAGAGT"
     gene            2921..>3696
                     /locus_tag="VFR25_03260"
     CDS             2921..>3696
                     /locus_tag="VFR25_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940098.1"
                     /translation="MSPRNRWAAALLAALLTAVIAVPALAWDGLPSYVDESNILSKTP
                     GTDDGAIGAIFNPAQWGVLEKGELDFYWSDTSVRPHAMDNWGLAFGQGAGMSIRRHDA
                     LLNGEPSSVTDWQIGAGGGTRGYYSGMSFGFSGPGKGAFNHDNYLGFGSISRPSRYLS
                     TGLVTRFGLSQGDIDGVFDVGIRPLGDPRLLLFGDYALSRGDRWDDGPLAGGVAIRPI
                     PGFEVAGRWGDQDRLQVTVGVTLQRAGFRATPQYDRDGNLG"
BASE COUNT          574 a         1313 c         1208 g          601 t
ORIGIN      
        1 gggctgggcc gggcccggtg acccccttcc cgcccatgcc tccggagctc gcgcgcctgc
       61 ccgagctgtc ccggaacctc tggtggtcct ggcattcgga agcacgcgac ctgttccgct
      121 cgctcgacga gacgctgtgg cgccggacgc gccacaatcc ggttcgcctc ctccaagaaa
      181 tcgaccccga ccgcctcgca cgcgccgccg cggacgcggg ataccgcgtg cagtacatcg
      241 cggtcatgcg cgccttcgac gccgcgctca acgacgagga cacctggtgc gaggaggagc
      301 atcccgatct cgccgacggc gtcctcgcct acttctcggc cgagtacggc gtccacaact
      361 cgctccccct gtacgcgggc ggtctcggcg ttctggccgg ggacatcacc aaggaagcga
      421 gcgacctcgg gctccccttc gtcggcgtcg gattcatgta cccccagggc tacttccgcc
      481 agcacgtgaa cgcggagggg cgccaagaag aggtctacga gcggatcgag cgggggcacg
      541 tcgccgtatc gccggttcgc acgccggacg gcgagccgct gcgcgtccgc ctctcgctcc
      601 ccgaccgaac gctccacgtc gccgcgtggc aagtgagcat cgggcggagc cggctcttcc
      661 tggtcgacac cgacctcgac gagaacacga cgggcgaccg cgagctgacg gggcgactct
      721 acggcggcga ccagaacgcg cgggtgagcc aggagatcct cctcgggatc ggcggcgtgc
      781 gcgtcctccg cgctctcgga atcaaaccga ccgtgtggca cggcaacgag gggcacacgt
      841 cgctcatgat gctcgagcga gtgcgcgagg agatcgcggc cggcctcacg ttcgacgaag
      901 cggtggacgc ggtgcggcgt acgaccgtct tcaccacaca cacgccggtc gccgcggggc
      961 atgacgcctt ctactacccg ctcatggagg ggcacctcga tccgatcgag gggtacggcg
     1021 tgaccgcgct cggcgaccac cgggccaagc tctacggcct cgcgtcctat cccgccgact
     1081 ggggcatcgc gttcaacatg acggcgctcg cccttcggct ctccggccac gtgaacgcgg
     1141 tgagccgcaa gcacggcgag gtctcccgcg cgatgtgggc accgctttgg cccagcaaga
     1201 agccggacga ggtgccgatc gtctcgatca cgaacggcgt gcacgtgccc acgtggatcg
     1261 cgaatgccac cgaccgatgt ctccgggaga atctcgatca ggactggctc cgccggcacg
     1321 acgacccggc tctgtggcag aagtcggagt cgattcccga cgaggcgctc tgggcgctcc
     1381 gcagccacct gaagctcgga ctgttccact tcctgcgcga tcggctccgc cagcgctggg
     1441 ccgcgcacga cgtggaagcg agccaggcgg tcgcgctcgg cgcgctactc gatcccgagg
     1501 cgctcacgat cggcttcgcg cggcggttcg cgtcctacaa gcgcgccacg ctcatccttc
     1561 gcgacctcga tcgcctgcac cacatcctcg acgacacgcg ccgccccgtg cagctcctct
     1621 tcgcgggcaa ggcccatccc gcggacgaag ggggcaagga tctgcttcag gccgtctatc
     1681 gcgccgcgcg cgatcccgtg ctcgcgggac gcatcgcgtt cctcgaggac tacgacatgc
     1741 acgcggcgca ctggctggtc cagggcgtcg atgtgtggct caacaatcca cgcgctccgc
     1801 aggaggcaag cggcacgagc ggcatgaagg cgggaatcaa cggcgtgccc agcttgagcg
     1861 tgctcgacgg ctggtggatg gaggcgtggg acgggaagaa tggctggggg ttcccggagg
     1921 ccgaggggga cgacgtggac gcccgcgacg tggagcagct ctactcgctc ctcgagaagg
     1981 agatcgtgcc cctctactac gaccgcggca tggacggcat cccgcgcggg tggctcgagg
     2041 tcgtgcggca cgcgctccgg accatcaccc ccgcgttctc cgcacggcgg atggtgaagg
     2101 agtacgtccg gacgatgtac gcgcccgcga tggccggcaa gccggtcctg tccgcaccac
     2161 cagcaccctc cgccccgacc gactgatttt cctgtaaagt cgtcccctct atgcctttcg
     2221 gagtggagtg gcacgacttc gtcacgatcg gtctcctcgt ccttctcgag ggggccctga
     2281 gcgcggacaa cgcgctcgtt ctggccgttc tcgtccttcc gctcccgccc gagcagcgca
     2341 cccgggccct ccgctacggc attctcggcg ccttcgcctt ccgaatcctt tccacccttt
     2401 tcgccaccta catgatccac tgggactggg tgaagctgat cggcgccctg tacctgctct
     2461 acctgccggc gaagcacttc atcgatcacc cggagggcaa gcagggcggg gccaaggcgg
     2521 cgatcaagac attcctcgga ctctccgcgt tctggagcac cgtcgtgcgc gtggagctga
     2581 ccgacatcgt gttcgcgatc gactcgatcc tcgtggccgt cgcgatgtcc cggaaggcat
     2641 gggtcatcgt gatgggcggc atcttgggga tcctggcgat gcgactcctg atcgggcagc
     2701 tcctcggcat cgtgcagcga tatcccgcga tcgtggacgg cgcgtacgtg atcgtggcgt
     2761 gggtcggaat caagctgatg atcgagtatt tccattccat cgggctgatc gcctgggagg
     2821 ttccgcggtg gctctccatc acggtggtgt tcgtgctgtt cgggatcagc gccctctacg
     2881 cgcgctggaa ggagcagaag aaggtagcgg gagccgggac gtgagccctc gcaatcgctg
     2941 ggccgcggcc ctcctcgccg ctctcctcac cgccgtgatc gctgtccccg ccctcgcatg
     3001 ggacggcctc ccgtcctacg tcgatgaatc caacatcctc tccaagaccc ccggcaccga
     3061 cgacggagcg atcggcgcga tcttcaatcc cgcgcagtgg ggcgtcctcg agaagggcga
     3121 gctcgacttc tactggtccg acacgagcgt gcggccgcac gcgatggaca actggggcct
     3181 cgcgttcggc cagggcgccg gcatgtcgat tcgccgccac gacgctctat tgaacggaga
     3241 gccgagctcc gtcaccgact ggcagattgg agcgggcggc ggcacgcgcg gttactacag
     3301 cggcatgtcg ttcggcttct cgggcccggg caagggcgcg ttcaaccacg acaactatct
     3361 cggcttcggc agcatcagcc ggcccagccg ctatctcagc accggactcg tcacgcgctt
     3421 cggcctctcc cagggggaca tcgacggcgt gttcgacgtg ggcatccgtc ccctcggcga
     3481 tccccgcctc ctcctcttcg gcgactacgc gctctcgcgc ggcgatcgct gggacgacgg
     3541 cccgctcgcg ggcggcgtcg cgattcgacc catcccaggc ttcgaagtcg cgggacggtg
     3601 gggtgatcag gatcgactcc aagtgaccgt cggcgtgaca ctccagcgcg ctggattccg
     3661 cgccacaccg caatacgacc gcgacggcaa cctcgg
//
LOCUS       DASTKC010000073         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_523773, whole genome shotgun sequence.
ACCESSION   DASTKC010000073 DASTKC010000000
VERSION     DASTKC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523773"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFR25_03265"
     CDS             complement(<1..680)
                     /locus_tag="VFR25_03265"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET9940099.1"
                     /translation="MRIAIVIALCLLVLPLTAEGAQIFEKVGTLGGQSLKMGVGARAN
                     AMGDSYVAIADDASAVYWNPAGISRLSGQSITLNHTAWPADVSFDQAGYVFNIKWIPG
                     MLGVNVRALTMSRDIVRTTYLPDGNGETFDAGEWTYGLSYARSLTDKFSAGFNINYVQ
                     TGLDDVKGKSVTFDFGTLYDVGVLGAKIGMAIQNIGSDMTFIDEKVKMPVFFRVGGSA
                     ALMQSGESR"
     gene            complement(722..3514)
                     /locus_tag="VFR25_03270"
     CDS             complement(722..3514)
                     /locus_tag="VFR25_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940100.1"
                     /translation="MSQAGRSVVGMMRAFRGGLAVVSVAAVASLCGNASAYYEPPPGS
                     TETVRLPANRTFRLDGKFVHNVGRLHLQVTNIGQTGNAANPSRTTVPSAEYPPGSGVD
                     HLYAAGLWVGAVDASGIPHVTTAAYAVEFSPETNPENPCVNLPIDQVADVRESYEGIP
                     GGNRIISSSLNPDDDGDGRIDEDFLNGLDDDGDGLCDEDYAAIGQQMFACEYRDDLPA
                     IRQINPEHVPLGIKVRQTSYAWSTPGQSDFVGMDYRIQNLSGRALRNVFVAIFADPDI
                     GNRAVDGYFQDDEAALFDSTVSYIGSTGEVVKRRIQMAYAWDNPDNPNRPQDNKGGDV
                     PGDGYFGCMFLNHTTDPAGVNAPPRVGITSFKFFSGSASFASGGDPENDAQRYQLMSD
                     PRLNPNNSFNPNFAVTQSSQRPLDYRFLMATGPFRQILPDSTLTISVAWVAGLGLGRD
                     TGGGTSTPSSLVANAVSAQQVFDGLYADLDNNLTTGSCGKETCLRNSQTGRFFTYEIP
                     DSSVCKRRFRSDPDAPDHDGRYADIPPWDANTSCADQQQGKYVCTDAVSPLQQHCASA
                     DTLDLTITSTECTYVDFDCDVNTGLGGREHLINWVAAAPLPSPFYSGVNGNNEVGDFL
                     PNYATHADSVRVSAWFFPGNNQITLKWSNFAEGIRDAQRGNLKEFIGYRIYKAAGWDR
                     PLGTNAPSRELWTLLGEWRVDPKGVSAHPLGDLIDPAAGTVGIDTVEVRWDPVNRRVD
                     NDPTHAHTEIDTLYSIGRYSYVDHNVLNGFPYFYSIVPVSVVPGDVPANDVLLSGNPG
                     ATNSQVVYPRGDSQPTTKEVYVVPNPYKAGAQWDLVPREEDPSGTKVVFVNLPKTKGT
                     IHIYTLAGDLVKDIPFDGNVPADLQFGKDPLTTGQGSVPWNLISRNGQKIVSGIYLYS
                     VDTDLGRQVGKFVVIR"
     gene            complement(3538..6264)
                     /locus_tag="VFR25_03275"
     CDS             complement(3538..6264)
                     /locus_tag="VFR25_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9940101.1"
                     /translation="MPSLRNRHRGGTPVLASRSPRILALLLAAATFAFAGSAHAQTGT
                     IQGKVVDESGMPLQGVNVVLVGLQTGAVTNAAGEFKIARVPVGTFTLRAMIASHERLD
                     RPVKVDANRTSEISFALHEKVTQIKGIEVKGDVKIAINKRSSATKQTVTSEDLRSLPV
                     DSYKDAIGLKAGVISQGGELHFRGGRANEVLTIVNGIPSRNPLSAEGVDLGLLAVSSS
                     EQVLGGLDAQYGNALSGVINLTTREGGDHLAGEVRYFTDRFGESDKSFTNYERLSLGV
                     GGPFFFPKTNFFVSVEGTFSDTYLKNTAETAEHRFLDFIRIGNRQSNQTNISSKLTYK
                     VTPNAKLNTEVIYNKSSRSPYHNRWNRKGFVQVRYDSTAPTDGSIATRYGTWAYYQVD
                     PSYQESNTAQHLPVTDEDYTSLAFTWRQTLAPGTIYNLRASRQEWRYLDDVLGRQLWE
                     YEQTPANYYDPFNRVDGQYYVTNGDYPHYESRFTTTYTLNGDFSKRVGMHNIMTGGDL
                     SYNSLTFLNTDFPNVMNPDGSYGATRDQFQYYNPEGSAFMQDRWEYEGMVLNAGLRYD
                     AFSVGEQIPSAEVTDRVKTQWSPRVGIAYPISDRDVMSFHYGRLFQVPERLNIYQGRN
                     ISAQARGNPNLEPQTTISYQLGVQHLFSKEIYGQFGVYFKDIFGLLTTVDQEVPGFAI
                     TVPTRVNGDYASSRGIEMTLIKRHSHGFSGEVNYTYGNATGTASDPNRALASSGNLSD
                     QYKPTSEQPLEWDQRHSLSATLRLGNEKDWAASFVYQFGTGFPYTPEQREQRRQDPAL
                     VNSQRLPSTSTLSVQAERFFRVWGQSVTFYLQGTNLLDAENITELQPSLWPNNGVNSN
                     SYTVYYTETGRAGGAFLTQDLDGDGREDWFPVNDPRVFQQGRVIRLGLGVQF"
     gene            complement(6565..>6789)
                     /locus_tag="VFR25_03280"
     CDS             complement(6565..>6789)
                     /locus_tag="VFR25_03280"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET9940102.1"
                     /translation="VEPKSVTVTGMTRDGLFAIEKGRISHAIKNFRFNQGVVEMLQQV
                     DGMTPSVRAGDTVCPGLRVRGFRMSSATEF"
BASE COUNT         1128 a         2103 c         2242 g         1316 t
ORIGIN      
        1 cggctctcac cgctctgcat gagggccgcc gagccgccca cgcggaagaa gaccggcatc
       61 ttcaccttct cgtcgatgaa ggtcatgtcg cttccgatgt tctggatcgc catgccgatc
      121 ttggctccca gcacgcccac gtcgtagagg gtgccgaagt cgaacgtgac cgacttgccc
      181 ttcacgtcgt ccaggcccgt ctgtacgtag ttgatgttga acccggccga gaacttgtcg
      241 gtgagcgagc gggcgtagga gagcccgtag gtccactcac ccgcgtcgaa agtctcgccg
      301 ttcccgtccg gcagataggt cgtccggacg atgtcccggc tcatcgtgag ggcgcgcacg
      361 ttcacgccca gcatgcccgg gatccacttg atgttgaaca cgtagccagc ctggtcgaac
      421 gacacgtccg cgggccacgc tgtgtgattc agggtgatgg actgccccga cagacgcgag
      481 atgcccgccg gattccagta caccgccgac gcatcgtcgg cgatagccac gtaggaatcc
      541 cccatcgcgt tcgcgcgggc gcccacaccc atcttgagcg attgcccgcc cagggttccc
      601 accttttcga agatctgcgc tccttccgcc gtcaggggca gcacgagcag acagagtgcg
      661 atcacgatcg cgattctcac gccattcctc ctcggttccc ggtgggtcgc tccggccggt
      721 ctcatcgaat caccacgaat ttgccgactt gccgccccag atcggtgtcg actgaataga
      781 gatagatgcc gctgacgatc ttctgtccgt tgcgggagat cagattccac gggacggacc
      841 cctgccctgt cgtcagcggg tccttgccga actggaggtc ggccgggacg ttgccgtcga
      901 aggggatgtc cttcacgagg tcgcccgcga gcgtatagat gtgaatggtg cccttggtct
      961 tgggcaggtt cacgaacacc accttggtcc cggacggatc ttcttcacga ggcacgagat
     1021 cccactgggc tcccgccttg tacggattcg ggacgacgta gacctccttc gtcgtcggct
     1081 gggaatcccc gcgcggatag acgacctggg aattggtcgc tcccggattg ccgctcagga
     1141 gcacgtcgtt cgctggaacg tcgcccggca cgaccgacac cggaacgatg gagtagaagt
     1201 aggggaaccc gttgagcacg ttgtgatcga cgtagctgta gcgtccgatc gagtagagcg
     1261 tgtcgatctc cgtatgggcg tgggtcgggt cgttgtccac acgccggttg accggatccc
     1321 acctgacttc gaccgtgtcg atgcccacgg tccctgcggc gggatcgatc aggtcgccga
     1381 gcgggtgggc ggacaccccc ttcgggtcca cgcgccactc acccaagagc gtccagagct
     1441 cccgagacgg tgcattcgtt ccgagcggac ggtcccatcc ggccgccttg tagatgcgat
     1501 agccgatgaa ctctttcaag ttgccgcgct gcgcgtcccg gatcccctcg gcgaaattgc
     1561 tccacttgag ggtgatctgg ttgttccccg ggaagaacca ggccgagacc cggaccgaat
     1621 ccgcgtgcgt cgcgtagttc ggcaggaagt cgcccacctc gttgttgccg ttcacccccg
     1681 agtagaacgg ggacggcagc ggtgcggcgg ccacccagtt gatcaggtgc tcccgtccgc
     1741 cgagtccggt attcacgtcg cagtcgaagt cgacgtacgt gcactcggtg gatgtgatgg
     1801 tcaggtccag cgtgtcggcc gaagcgcagt gctgctggag cggcgagacc gcgtccgtgc
     1861 agacgtactt gccctgctgc tgatcggcgc acgaggtgtt cgcatcccag ggcggaatat
     1921 ccgcataacg gccatcgtgg tccggagcgt ccggatcgct gcggaaccgg cgcttgcaga
     1981 cagaggaatc aggaatttca tacgtgaaga accggccggt ctgggaattc cggaggcagg
     2041 tctccttgcc gcaggacccc gtcgtcaggt tgttgtcgag gtccgcgtag aggccgtcaa
     2101 agacctgctg cgcggagacg gcgttcgcca cgagcgagct cggcgtgctg gtgccgcctc
     2161 cggtgtcgcg ccccagcccc agccccgcca cccacgcgac cgagatcgtg agggtcgagt
     2221 ccggaaggat ctggcggaag ggtccggtcg ccatgaggaa ccggtaatcc agaggtcgct
     2281 gcgacgactg cgtgaccgcg aagttcggat tgaagctgtt gttgggattg agcctcggat
     2341 ccgacatgag ctgatagcgc tgggcgtcgt tctccgggtc gccgcccgag gcgaacgagg
     2401 cggagcccga gaagaatttg aacgacgtga tcccgacccg cggtggcgcg ttgaccccgg
     2461 ccggatcggt cgtgtgattg aggaacatgc agccgaagta gccgtctcca ggaacatccc
     2521 cgcccttgtt gtcctgcggc cggttgggat tgtccgggtt gtcccacgcg tacgccatct
     2581 ggatgcgccg cttcaccacc tcgccggtcg agccgatgta cgagaccgtc gagtcgaaga
     2641 gggcagcctc gtcgtcctgg aagtagccgt ccaccgcgcg gttcccgata tcggggtcgg
     2701 cgaagatggc gacgaagacg tttcgaagcg cccggccgct caggttctgg atccggtagt
     2761 ccatgccgac gaagtcgctc tgccctgggg tcgaccacgc gtagctggtc tgacgcacct
     2821 tgatccccag gggaacgtgc tcgggattga tctggcggat ggccggcaga tcgtcccggt
     2881 actcgcaggc gaacatctgc tggccgatgg cggcgtagtc ctcgtcgcaa aggccgtcgc
     2941 cgtcgtcgtc gaggccattc aggaagtcct cgtcgatgcg gccgtcgccg tcgtcgtccg
     3001 ggttcaagct gctcgagatg atccgattgc cgcccgggat gccctcgtag gactcccgca
     3061 cgtcggccac ctgatcgatg ggaaggttga cgcaggggtt ctccggattg gtctccggag
     3121 agaactcgac ggcgtacgcc gccgtggtca cgtgcgggat tccgcttgcg tccacggccc
     3181 cgacccagag gccggcggcg tagaggtggt cgacgcccga tccgggcgga tattcggccg
     3241 agggaaccgt cgtccgcgac gggttcgccg cgtttccggt ctggccgatg ttggtcacct
     3301 gcaaatgcag gcggccgacg ttgtgcacga acttgccgtc gagccggaac gtccggttgg
     3361 caggaagcct cacggtctcg gtcgatccgg gcggcggctc gtagtaagcg gaggcatttc
     3421 cacagagact ggcgaccgcc gcgacggaga cgactgcgag tccccctcgg aaggcccgca
     3481 tcatccccac caccgatcga cctgcctggc tcatggtgtc tggctgtctc gtcatggtca
     3541 gaattggacc cccagtccca accggatcac gcgtccctgc tggaacacgc gcggatcgtt
     3601 gaccgggaac cagtcttcac gaccgtcccc gtctagatcc tgggtcagga aggcaccgcc
     3661 ggctctgccc gtctccgtgt aatagaccgt gtacgaattc gagttcaccc cgttgttcgg
     3721 ccagaggctc ggctggagct cggtgatgtt ctccgcgtcg agcaggttcg tgccctggag
     3781 gtagaacgtg acgctctgcc cccagacgcg gaagaaccgc tccgcctgga ccgaaagggt
     3841 ggacgtcgag gggaggcgct gcgagttcac gagtgcggga tcctgccggc gctgctcgcg
     3901 ctgctccggc gtgtacggga agcccgtccc gaactgatag acgaacgagg ccgcccagtc
     3961 cttctcgtta ccgagccgga gcgtggccga gaggctgtgc cgctgatccc actcgagcgg
     4021 ctgctccgag gtcggcttgt actgatcgct caggttgccc gagctcgcca gggcgcggtt
     4081 ggggtcggac gcggtcccgg tggcattgcc gtaggtgtag ttgacctcgc ccgagaagcc
     4141 gtggctgtgg cgcttgatca gggtcatctc gatgccgcgc gaggaggcgt agtcgccgtt
     4201 cacgcgggtc ggcacggtga tggcgaagcc cggcacttcc tggtcgaccg tcgtcaggag
     4261 gccgaagatg tctttgaagt agacgccgaa ctgaccgtag atctccttgc tgaacaggtg
     4321 ctgcacgccg agctggtagg agatcgtcgt ctgcggctcg aggttcggat tgccgcgcgc
     4381 ctgggcagaa atattccggc cctggtaaat gttgagccgc tccggcacct ggaagaggcg
     4441 gccgtagtgg aagctcatca cgtcccggtc ggagatcggg taggcgattc cgacgcgcgg
     4501 gctccactgc gtcttcaccc ggtcggtcac ttccgccgaa gggatctgtt cgcccacgga
     4561 gaaagcgtcg taccggaggc ccgcattcaa aaccattccc tcgtactccc agcggtcctg
     4621 catgaacgcc gaaccctcgg ggttgtagta ctggaactgg tcgcgcgtgg cgccgtagct
     4681 cccgtccggg ttcatgacgt tcgggaagtc ggtattgagg aaggtgagcg agttgtagga
     4741 gagatccccg cccgtcatga tgttgtgcat cccgacccgc ttcgagaagt cgccgttcag
     4801 ggtgtaggtg gtcgtgaagc gcgactcgta atgcgggtag tcgccgttcg tgacgtagta
     4861 ctgtccgtcg actcggttga acgggtcgta atagtttgcg ggcgtctgtt cgtactccca
     4921 gagctgtcgg ccgaggacgt cgtcgaggta tcgccactcc tgccgcgaag ccctcaggtt
     4981 atagatcgtt cccggcgcca gcgtctggcg ccaggtaaac gcgagcgacg tgtagtcctc
     5041 gtcggtgacc ggtagatgct gcgcggtgtt cgattcctgg tagctcgggt cgacctggta
     5101 gtaggcccag gtgccgtagc gcgtcgcgat cgagccgtcg gtcggggcgg tcgagtcgta
     5161 tcggacctgg acgaaaccct ttcggttcca gcgattgtgg tacgggctcc gcgaactctt
     5221 gttgtagatc acttcggtgt tgagcttcgc gttcggcgtc accttgtagg tcagcttgct
     5281 cgagatgttg gtctggttcg actgccggtt gccgatccgg atgaaatcga gaaagcgatg
     5341 ctcggccgtc tcggcggtgt tcttgagata cgtatccgag aaggtcccct cgaccgagac
     5401 gaagaagttg gtcttcggga agaagaaggg gccgccgacg ccgaggctca gccgctcgta
     5461 gttcgtgaac gacttgtccg actcgccgaa gcggtcggtg aagtagcgga cctcgccggc
     5521 cagatggtcg cccccctcgc gagtcgtgag gttgatgact cccgagagcg cgttgccgta
     5581 ctgggcgtcg agcccaccga ggacctgctc gctcgaggag accgcgagga gcccgaggtc
     5641 cacgccttcc gcggagagcg gattgcggct cgggatgccg ttcacgatcg tgaggacctc
     5701 gttggcgcgg ccaccgcgga agtggagctc gcccccctgg ctgatgacgc cggctttcag
     5761 gccgatggcg tctttgtacg aatcaaccgg cagcgatcgg aggtcctcgc tggtgacggt
     5821 ctgtttggtg gcggagcttc gcttgttgat ggcgatcttg acgtcgcctt tgacctcgat
     5881 ccccttgatc tgggtcacct tctcgtggag cgcgaacgaa atctcgctcg tccgattcgc
     5941 gtcgaccttg acaggtcggt cgaggcgctc gtggctcgcg atcatggcgc gaagagtgaa
     6001 cgtgccgacg ggcacgcgcg cgatcttgaa ctcaccggcg gcgttcgtca ccgcgcccgt
     6061 ctggagtccg acgagcacga cgttcacgcc ttggagtggc atcccgctct cgtccaccac
     6121 cttgccctgg atggttcctg tctgagcatg ggcggagccg gcgaaggcga acgtggccgc
     6181 ggcgagaagg agcgcgagaa tcctgggaga gcgggacgcg aggacggggg tcccgccacg
     6241 atgtcgattc cgcaagctcg gcatggctcg gtctgctcct ttcctggctg actcaggacc
     6301 tgacgaaggg gccgatatta cgtcttcgtg accttcgtcc gtcaaggcaa atcgttgaac
     6361 caaccctggt tgtggcaatc ttaggctcgc tattcggccg ggcagcgcag accaatagac
     6421 ggcatggtac ggggggacga aaccaaaacc ggcggggggg acttcggtcg aagcgccgat
     6481 gggtcgggaa gcgcttctac cgcgaaatta tatccgcttc gtaacggtgc gcaagccctt
     6541 tttaccagtt cttcccgatg ccggtcagaa ttccgtcgcg ctcgacatcc gaaatccccg
     6601 gacccggagc cccggacaga ccgtgtcgcc ggccctaacg gaaggcgtca tcccgtcaac
     6661 ctgttggagc atctccacga ctccctggtt gaaccggaag ttcttgatag cgtgactgat
     6721 cctgcccttc tcgatggcga agagcccatc gcgggtcatc cccgtcacgg ttacgctctt
     6781 cggctcgac
//
LOCUS       DASTKC010000074        14416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_998313, whole genome shotgun sequence.
ACCESSION   DASTKC010000074 DASTKC010000000
VERSION     DASTKC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998313"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFR25_03285"
     CDS             complement(<1..183)
                     /locus_tag="VFR25_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9940103.1"
                     /translation="MNPKDTTRLWEELRHRDPDEKSEWIRQLAEQPSPGSVDVLLDVL
                     KQESWYLRDQAARALAT"
     gene            complement(188..1222)
                     /gene="obgE"
                     /locus_tag="VFR25_03290"
     CDS             complement(188..1222)
                     /gene="obgE"
                     /locus_tag="VFR25_03290"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET9940104.1"
                     /translation="MLADRASIHVVAGRGGNGCVSFRREKYVPKGGPDGGDGGDGGSV
                     ILVVNPHMRTLLDFQSRTLFRAQAGAHGSGNQKFGRGGEDLRVPVPLGTLVIDDESGE
                     TLADLVGPEMEFPAARGGRGGRGNARFARPTHQAPRTAEQGTAGEDRRLRLELRLIAD
                     VGLVGFPNVGKSTLLSSISAARPKIADYAFTTLEPHLGLVRVHDEDSFVMADLPGLIE
                     GAHQGKGLGHEFLRHIWRTRVLVVLIDALSEDPARDLVVLRNELHSYHPEFAHKPMIV
                     ALSRSDLLGGAAPESPPLPLDGATWGGAISGVTGEGTLALKERIWSVLAREGLASPDA
                     DATNAAGTQE"
     gene            complement(1235..2314)
                     /locus_tag="VFR25_03295"
     CDS             complement(1235..2314)
                     /locus_tag="VFR25_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET9940105.1"
                     /translation="MSYRVTLIPGDGTGPELAQATRRVLEATGIKFDWDIQDAGMDVY
                     EKEGTPLPERVLESLRKNKVGLKGPITTPVGSGFRSVNVALRKELDLYACERPCKSYP
                     GVRSRYEAIDLVIIRENTEDLYAGIEFPVGSQGAQALRDLVRREANREIKEDSGISIK
                     PISVSGTERIVRHAFRYARANGRHSVTAVHKANIMKNTDGLFLETARRVSQEFPGIEF
                     QDRIVDNMCMQLVQTPENYDVLVLPNLYGDIVSDLAAGLVGGLGMAPGANIGQDAALF
                     EPTHGSAPKYAGQDKVNPFAMILSGMLMLRHLGEKDAADRLERAVAGVVKRGKQVTYD
                     MKARRDDPTAVGTRAAADAVIEALD"
     gene            complement(2474..2767)
                     /locus_tag="VFR25_03300"
     CDS             complement(2474..2767)
                     /locus_tag="VFR25_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET9940106.1"
                     /translation="MPTYEYECLKCGKRFEVFQKMTDEPKKRCPTCRGKVRRLIGAGA
                     GMIFKGSGFYITDYRSKSYKEQQKKESGAPAPAKDEPKSQPKPKPKESAPKTQ"
     gene            complement(2780..3988)
                     /locus_tag="VFR25_03305"
     CDS             complement(2780..3988)
                     /locus_tag="VFR25_03305"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9940107.1"
                     /translation="MSPSQAGTRERVSARVRALKPSETMALSARARELRARGEKVISF
                     AAGEPDFDTPEHIRLAAVEAVHGGHTRYTEVAGVAPLREAIAAKLARENGVRYEPKEI
                     VVSNGAKHAIWNALFTLLEPGDEVLCPVPYWVTFPEVVRLIGATPVPVPPGSGRYKVA
                     PADLERALTPRTRLLVLNSPNNPSGAVYTRREIESIADFVARHDLYVLSDEIYETLVY
                     GKAEHVSIASLSPELRERTVLVNGVSKTWAMTGWRLGYAAAPRDVADGMDRLQGQMTS
                     NPSSVSQQAALKALTGDRGPALSMRDRFAARRDLMVERLRAIEGVRLVEPEGAFYAFP
                     DVSERIRAARNGVTDSASLCDYLIDEAKIVCVPGSAFGMEGHLRLSYAVSERDIEDGL
                     DRLLAAFQGL"
     gene            complement(3985..4476)
                     /gene="coaD"
                     /locus_tag="VFR25_03310"
     CDS             complement(3985..4476)
                     /gene="coaD"
                     /locus_tag="VFR25_03310"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9940108.1"
                     /translation="MKSGVFAGTFDPVTNGHLDLIHRALGVVDRLVVAVAPRPEKGVL
                     FSLEERVAMIKEAVGVESRVTVEPFQGLLVEHAKARGIPIIIRGVRFVSDFEYEFQMA
                     LMNRRLDPTIETIFLMPSEQFTYVNSTLVKEIARHGGDVNPFVPPNVRERLDKALRKP
                     AAR"
     gene            complement(4473..5057)
                     /gene="rsmD"
                     /locus_tag="VFR25_03315"
     CDS             complement(4473..5057)
                     /gene="rsmD"
                     /locus_tag="VFR25_03315"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET9940109.1"
                     /translation="MGFVRVVGGALRGRKLQVLDRGVRPTSERTREAIFDILGPATVA
                     GARVLDLYAGTGALGTEALSRGARSTDFVEVDRAVARRLEESLAELGLAEVARVHVGD
                     LDRGLPAGIRGPWELVFLDPPYDGDAGARWVEAAARGGLLGAEGILVYERRKGSDAPT
                     PAGLTLRTDRIYGDTAVAFYRADVPEGRPGQGGL"
     gene            complement(5036..5545)
                     /locus_tag="VFR25_03320"
     CDS             complement(5036..5545)
                     /locus_tag="VFR25_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9940110.1"
                     /translation="MPGAGKSIVAPLLAARLGFTHVDLDQRIERDEQTTVADVIRSRG
                     EEAFRALEARALGSALEESAQGVVIACGGGILGRDENRERLARAFVLWLLVTPETAAA
                     RMGSAGRDSRPLLNGGSVLDRLRSLEAARSELYRRAASATVDTEGRSAAEVADLAAGL
                     WRAWDSSAS"
     gene            complement(5674..7350)
                     /locus_tag="VFR25_03325"
     CDS             complement(5674..7350)
                     /locus_tag="VFR25_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET9940111.1"
                     /translation="MRLETSKETGRRAGLLSAVAMGLLLASAVHASAATVKSISLKDA
                     GTGTQVIVEADSPINYHDFTLESPDRIVLDCPGSTLGFTERTWAGRDKSPIKSVRATE
                     MGWGKDTGSRIVVDLAKPTSYGVSAVGNTIVLALEQAAPEPPPAPEPVVSPSSSSSDN
                     MQPSDGRRISIDVQGAEVETVLRSLAEFSGRNIVASKEVKGTVTLRLRNVPWRQALDI
                     LLRTQALGMVDNGNNIVVSNLETLRKEELERRTAERAQEELLPLYTRIIPVSYANADE
                     MSKAVEKTLTKRGHIEVDKRTNALLVTDIDDRLSQAESMIRSLDTRTPQVEIVARLVD
                     VDRSASRSLGIDWTLSNLDIGDAGAHEGVAVTPGQVPSPAGTVKFGTVKGFGSIEATL
                     TALETQNKANIISNPRITTVNNREASVVVGQQIPLIVQDFAGNAVTQLTTIGIKLSVT
                     PHINVGNKITMDVHPEVSDLSSQATVQGGIIINTTMADTRVMVNDGETAVIGGLIRSN
                     ESDTYRGVPILMDVPLLGNLFKSSTKVKQKRELLIFLTPKILGETTASTE"
     gene            complement(7334..8125)
                     /locus_tag="VFR25_03330"
     CDS             complement(7334..8125)
                     /locus_tag="VFR25_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940112.1"
                     /translation="MNLPSSLALPQSKLFQSRIFLVLLLVLAVLIGAGVFYFMRPLTP
                     PKAPTVATSPAAQAVPRPTPVKAPAAPVPAPIVYGPPAPDWVERVPMPAAPGAPAAAA
                     TALPTGAATSTTPLSAASTGALPATGAAGIVAAQAQETPQSLDASVLFAREQYIYTSG
                     GRRDPFQSLLDGKYQSASGDGALVDVGDIHLVGIMWGSADKYALVEDSRGRGFVLRVG
                     DPVVNGYISGITKNEIQVVQNAFGESQTLSIQLKPKEGDENATRN"
     gene            complement(8088..8696)
                     /gene="pilO"
                     /locus_tag="VFR25_03335"
     CDS             complement(8088..8696)
                     /gene="pilO"
                     /locus_tag="VFR25_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET9940113.1"
                     /translation="MDFKDPKTQKLMLVGLGTAVLVYFYVFADYVPFNYKARSKEISA
                     LKADYTKESNELTKAQQLVNRLPELKKEFALLNQKWSVAQELLPTQKEVASLLRKVTI
                     AGQESGVKFVLFKPSEPHPTPDFTENPVQVTITGGFHHAGSFLAEIAELSRLVNVSQL
                     RLKGFDKGELDDTVQAEFVATAYTLAEGIQNEPAKQPGAAAK"
     gene            complement(8711..9271)
                     /locus_tag="VFR25_03340"
     CDS             complement(8711..9271)
                     /locus_tag="VFR25_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET9940114.1"
                     /translation="MIRINLLPRDERQVRRKIVLPNIGAMVPVFALLGVVGLLVACSI
                     VQAMQVARLKADIAHAREEAEKLRPQIQTIQELTQKREELTRRLNVIAALDKNRLWRV
                     KLVDELSKCVPDHLWLTNYEEIGPDQVKVEGVTFSNLLVADFMSRLEASPLYGDVDLV
                     IAERGQIDQRNVVKFTITAKMTFQPA"
     gene            complement(9281..10309)
                     /gene="pilM"
                     /locus_tag="VFR25_03345"
     CDS             complement(9281..10309)
                     /gene="pilM"
                     /locus_tag="VFR25_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET9940115.1"
                     /translation="MFGKKKSTVGLDIGSSSIKVVEIARDRSGDHLINYGVSEPLSEA
                     IVDGEIMDRQMVHEAITNLMESRQIRTKSVATAVSGRAVIVKKILMDRLNEEDAKEAI
                     QWEAEQHVPYDINDVSLDFQIINPNIDAKKMQVLLVAAKKDMVQNHAEIIREAGLTPT
                     IIDVDSFAIQNAIEANYEFLPGETVALVNIGAEITNLNIVRDGVPHFTKDLSVGSNSF
                     VEGIQRRHNVSQPEALAALRGRNESGIDVTAIVQTASEDLSVQIDRAISFLKSAGDAD
                     RIDRILVSGGSARITGLTDFLSGRHHVPVSVVNPVQRLSFDPGLFGGDMPEAVAPMLT
                     VAIGLALR"
     gene            10619..12181
                     /locus_tag="VFR25_03350"
     CDS             10619..12181
                     /locus_tag="VFR25_03350"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9940116.1"
                     /translation="MFRHSRGAAWVAALVALWVYASTLSPTVAWRNFGEDSGDLLVAS
                     ATLGIPHPTGYPLYVLLGRVSSLLPIGTTAFRINLVSALAGAAGVFFIARLAAELAPA
                     AGSAALAIAALAAALLYAFSQGAWSQSVVAEVYTLNIVFLAAILWALARSERSGKNRY
                     LALAAYLFGLGLSNHLLVLAAAPAIAWVAVRRLVSKSIGVTGCVLLLVLVIWGVSLDA
                     YLPIRASLGPEFSWGVPNTPQRLWWVLTGAQYRGNFFSRGFVAVGSHLFPGRWWMDFG
                     PGLLVLLGGVAALLFLARRPRQDGAEGAPNAGASAVPALLALAASLVLLSSYTIPDDV
                     GYWMPAGAILCCLAGAGLGGAWERVPFLGRAALGIAAAALVLLMARANWPLVDASNDM
                     TPYLYARRNLEAVEPNALIVSDYDGRTFSLWFYKATEFKQSHPKLAVVYKSLLIWDWY
                     RYHIAKRYPGLVAPSGPPDPNLMLNRIIARNIKNRPVYIVRPDTALVTLFKTEPVGYP
                     PMPIYRVRLVEP"
     gene            12178..14295
                     /locus_tag="VFR25_03355"
     CDS             12178..14295
                     /locus_tag="VFR25_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940117.1"
                     /translation="MIESVRELLRQRGGILLTLLILAVTAFHVGSMLRTELTVTGGGL
                     SLPLDDSFIYLQYARAIAQGHPFVYTPGNAPTTGATSLGYPLLLLPPHLLRLPPSICI
                     AWTLAIGVVGFVASALLILRLGRRLGGCVGGGLALALFLASAQLLWGYMSGMEVPLYA
                     TVLLATLHTYLRERKEARFPKLRWWLLALALSRPEGAVLCFVFGLLVLADRHRAARAG
                     APLRLAEPISILPFLFGLLPFLVNLAVAGSIESTSSQAKSILAEPYRDVRASYIENAP
                     KVWSDIAASYAGAFLLNERGLPFPRMTIPFAIGAGLYLAFALWPRARSWDRGLALFPL
                     VGAGILVNSIPVVWQVHLFRYQQGLYPVVLLLAAGGFGRLATWSWSTLPRAAGALIAA
                     IAVLAPVVAWSPLLLRAHAEIALFYAHNCENILHQQVRVGQWIDRNLPPNAIVGLNDA
                     GAIAYYGNRSTLDLVGLTTAGFAGVYRSGLGCLFEHLRRLPADRLPTYFAIYPGWFPY
                     WEESGILGPEAYRAHLGINTITGGTDMVVYPASWIDVEPTDHPVLPHREIEGKRPVDS
                     IDLAWLEDESRHEWSSDPEATDVLRRYAYADVRNRPITDAGRIVRKSERFRASVTPGR
                     DLTLVMRTDAWYPTHLDVQVDGAPAGTWSIARSESTWVEPTFTIPARLIQHPRSEIGL
                     TRQVTTDVEAQRDYAPFHYWLYQ"
BASE COUNT         2081 a         5030 c         4735 g         2570 t
ORIGIN      
        1 cgtcgcgagc gcgcgcgccg cctggtcgcg caaataccag ctctcctgct tcagcacgtc
       61 gagcagcacg tccaccgagc cgggtgaagg ctgttcggca agctgtcgaa tccactcgga
      121 tttctcgtcc ggatctctgt gacgcagctc ttcccagagt cgcgtcgtgt ccttcgggtt
      181 catgttctca ctcctgggtg ccggcagcgt ttgttgcgtc tgcgtcgggg ctggctaggc
      241 cctcccgcgc gagcacggac cagattcgtt ctttgagcgc cagggtgccc tcccctgtga
      301 cccccgagat ggcccctccc cacgtcgcgc cgtcgagtgg gagcgggggc gattccggcg
      361 ccgcgccccc caggaggtcg ctccggctga gggcgacgat catgggcttg tgcgcgaact
      421 ccggatggta cgagtgcagc tcgtttcgaa ggacgacgag atccctcgcg ggatcctcgg
      481 acagggcatc gatcaggacg acgaggacgc gagtcctcca gatatggcgc aggaattcgt
      541 ggccgagccc cttcccctgg tgggctccct cgatcaagcc ggggagatcc gccatgacga
      601 agctgtcctc gtcgtggacg cgtacgaggc ccaggtgagg ttcgagggtg gtgaaggcgt
      661 agtcggcgat cttggggcgg gcggcgctga tgctcgagag gagcgtcgac ttgccgacgt
      721 tcgggaaacc gacgagtccg acgtcggcga tgagccggag ctcgaggcgg agccgccggt
      781 cctcgcccgc ggtgccctgc tccgcggtgc gcggcgcctg gtgcgtgggc ctcgcgaagc
      841 gcgcgttgcc gcggccgccg cggccgccgc gcgcggccgg gaattccatc tcgggcccga
      901 ccagatccgc gagcgtctcg ccgctctcgt catcgatgac gagcgtgccg agaggcaccg
      961 gcacgcggag gtcctcaccg ccccggccga acttctggtt gccggagccg tgcgcccccg
     1021 cctgggcgcg gaagagcgtg cgggactgaa agtcgagcag cgtgcgcatg tgcggattca
     1081 cgaccaggat gacgctccct ccgtctcctc catcgccgcc gtccggtccc cctttgggga
     1141 cgtacttctc gcgccgaaag ctgacgcagc cgtttccccc gcgtccggcc accacgtgga
     1201 tggacgcgcg gtccgcaagc atccgggcgc tccgctagtc gagcgcttcg atgacggcgt
     1261 ccgccgcggc ccgtgtgccc acggcggtcg gatcgtcgcg gcgcgccttc atgtcgtacg
     1321 tgacctgctt cccccgcttc accacgccgg cgaccgcgcg ctcgaggcga tcggcagcgt
     1381 ccttctcgcc caggtgccgg agcatcagca tgccgctcag gatcatcgcg aacggattca
     1441 ccttgtcctg ccccgcgtac ttgggcgcgc tcccgtgcgt cggctcgaag agcgccgcgt
     1501 cctggccgat gttcgcgccg ggcgccatgc cgagcccgcc cacgagcccc gcggcgagat
     1561 ccgagacgat gtcaccatag agattcggga gcacgagcac gtcgtagttc tcgggagtct
     1621 ggacgagctg catgcacatg ttgtcgacga tgcggtcctg gaactcgatg ccgggaaatt
     1681 cctgcgatac cctgcgcgcg gtctcgagaa agaggccgtc ggtgttcttc atgatgttcg
     1741 ccttgtgcac cgcggtcacc gagtggcggc cgttcgcgcg cgcgtagcgg aacgcgtggc
     1801 gcacgatgcg ctcggtgccc gagacgctga tcggcttgat cgagatgccg gagtcctctt
     1861 tgatctcacg gttggcttcg cgccggacga ggtcgcgcag cgcctgggcc ccttgcgatc
     1921 cgaccgggaa ctcgatcccg gcatagaggt cctcggtgtt ctcgcggatg atgacgaggt
     1981 cgatcgcttc gtaccgggag cggacgccgg gataggactt gcaggggcgc tcgcaagcgt
     2041 acaggtcgag ctctttccgg agggcgacgt tgacgctccg gaacccggaa ccgacggggg
     2101 tcgtgatcgg acccttcaga ccgaccttgt tcttgcggag cgattcgagg acgcgctccg
     2161 ggagcggcgt cccttccttc tcgtagacgt ccatgcccgc gtcctggatg tcccagtcga
     2221 acttgatgcc ggtggcttcg aggacgcggc gtgtggcctg cgcgagctcc gggccggttc
     2281 cgtcgcccgg aatcaacgtc acgcgatacg acatggactc ccttcctgac gcggatgagg
     2341 ctgcattcgt aatcggaaga aaatttgcga gttagaactg ccgttcgtac caccagggcg
     2401 ggctgacggg cggcggctcg atctgccggc aacgtagcag aggaaaacgc ggagagtcaa
     2461 aagctttcac cggttactgg gttttgggag cgctctcctt cggcttcggc ttgggttggg
     2521 acttgggttc atccttcgca ggagccgggg cgccggactc cttcttttgc tgctctttat
     2581 aggatttcga gcggtagtcg gtgatgtaga agccgctccc cttgaagatc atccccgcgc
     2641 cggcgccgat gaggcggcgt accttcccgc ggcaggtcgg gcagcgcttc ttcggctcgt
     2701 ccgtcatctt ctggaacacc tcgaaccgct tcccgcactt cagacactcg tattcgtacg
     2761 tcggcattga tcctccggtt caaagcccct ggaacgcggc gaggagccga tccagtccgt
     2821 cctcgatatc gcgctcggag accgcatagc tgagcctcag gtgcccctcc atcccgaacg
     2881 cggagccggg cacgcaaacg atcttcgcct cgtcgatcag gtaatcgcac aggctcgcgg
     2941 agtcggtgac gccgttccga gccgcgcgga tccgctcgct cacgtccggg aacgcgtaga
     3001 acgccccctc gggctccacg agacgcacgc cctcgatggc gcggagccgc tccaccatga
     3061 gatcccgtcg ggccgcgaag cggtcgcgca tcgaaagggc cggtccgcgg tcgccggtca
     3121 gagccttgag cgcagcctgc tgcgagacgc tcgacggatt ggacgtcatc tgcccctgca
     3181 gccggtccat cccgtcggcg acgtcccgcg gagccgctgc gtagccgagg cgccagccgg
     3241 tcatcgccca tgtcttggat acgccgttca ccaggacggt tcgctcccgt agctcagggc
     3301 tcagggacgc gatggagacg tgctcggctt tcccgtacac aagggtctcg tagatctcgt
     3361 cggagaggac atagaggtcg tgccgcgcca cgaagtcggc gatcgattcg atctcacggc
     3421 gcgtgtagac cgcgccggag ggattgttgg gactgttgag gacgaggagc cgggtccgcg
     3481 gcgtgagcgc gcgctcgagg tcggccgggg cgaccttgta ccgccccgag ccgggcggga
     3541 cgggcaccgg cgtcgcgccg atcagccgta cgacctccgg gaaggtgacc cagtatggga
     3601 ccgggcagag cacctcgtct cccggctcga gcagggtgaa gagcgcgttc cagatcgcgt
     3661 gcttcgcccc gttcgagacc acgatctcct tcggctcgta gcgcacgccg ttctcgcgcg
     3721 cgagcttcgc cgcgatcgct tcccggagcg gggcgacgcc cgcgacttcg gtgtagcgcg
     3781 tgtgcccgcc gtggaccgct tcgacggccg cgaggcggat gtgctccggc gtgtcgaagt
     3841 cgggctcgcc ggcggcgaag gagatgacct tctccccgcg cgcgcggagc tccctcgccc
     3901 gggcgctcag ggccatggtc tccgatggct tgagcgcgcg cacgcgcgcg ctcacacgct
     3961 cgcgcgtccc ggcctggctc gggctcatcg cgcggccggc ttccggagcg ccttgtcgag
     4021 ccgctcccgg acgttcgggg gcacgaacgg attcacgtcg ccgccgtggc gcgcgatctc
     4081 cttcacgagg gtcgaattga cgtacgtgaa ctgctcgctc ggcatgagga agatcgtctc
     4141 gatggtgggg tccaggcggc gattcatgag cgccatctgg aattcgtact cgaagtccga
     4201 cacgaagcga acgccacgga tgatgatcgg aatcccgcgc gccttcgcgt gctcgacgag
     4261 gaggccctgg aagggctcga ccgtgacgcg cgactccacg cccacggcct ccttgatcat
     4321 cgcgacccgc tcctcgagag aaaagagcac tcccttctcc ggccgcggtg cgaccgccac
     4381 gacgagccgg tccacgacgc cgagcgcgcg gtggatgagg tcgaggtggc cgttcgtgac
     4441 cgggtcgaag gtgcccgcga acacgccgct cttcataggc ctccttgtcc gggacgcccc
     4501 tcggggacgt ccgcacgata gaacgcaacc gccgtgtcgc cgtagatccg gtcggtccgg
     4561 agcgtgagcc cggccggagt gggcgcgtcg cttcccttcc gccgctcgta cactagaatc
     4621 ccttccgcgc cgagcaggcc gccccgcgcg gccgcctcga cccagcgcgc tcccgcgtcg
     4681 ccgtcgtacg gtgggtcgag gaacacgagc tcccacgggc cgcggatccc tgccggaagt
     4741 ccccgatcga ggtcgcccac gtgcacgcgg gcgacctcgg ccaggccgag ctccgcaagg
     4801 ctctcctcga gcctacgcgc cacggcgcga tcgacctcga cgaagtccgt gctcctggcg
     4861 ccgcggctca acgcttccgt gccgagggct ccggtgcccg cgtacagatc gagcacgcgc
     4921 gcccccgcga ccgtagccgg ccccaggata tcgaagatcg cctcccgcgt gcgctccgac
     4981 gtgggcctga ctccgcggtc gagcacctgg agcttccgcc cccggagcgc gcctcctacg
     5041 acgcggacga atcccacgcc cgccacaggc ccgcggccag gtccgcgacc tcggccgcgc
     5101 tccgtccctc ggtatccacg gtcgcgctcg cggcgcggcg gtagagctcg ctccgagccg
     5161 cctccaggga acgcagccgg tcgagtacgc tgccaccgtt gaggagcgga cgcgaatccc
     5221 gtcccgcgga tcccatccgc gccgcggccg tctcgggcgt cacgaggagc cagagcacga
     5281 aggcccgcgc cagccgctcg cgattctcgt cacgccccag gatccctccg ccgcacgcga
     5341 tcacgacccc ttgcgcgctc tcctcgagcg cgctcccgag cgcccgcgct tcgagggcgc
     5401 ggaacgcctc ctcgccgcgg ctccggatca cgtcggcgac cgtcgtctgt tcgtcccgct
     5461 cgatccgctg gtccagatcc acgtgggtga agccgagcct ggccgcgagg agcggagcca
     5521 cgatgctctt tccggccccg ggcaatccga tcagagcgat tcgcgcggac agcgtggcct
     5581 ccgaagccga ctgaaacgag aacggggccc tccgtttcgg aagggccccg cagtataccg
     5641 aggtcgaggc gggccgaaca gcgcccgcga cgcttactcc gtcgacgccg tcgtctcgcc
     5701 gaggatcttc ggcgtgagga agatgaggag ctcgcgcttc tgcttcacct tcgtgctcga
     5761 cttgaagagg ttgccgagga gcgggacgtc catgaggatc ggcacgccgc ggtaggtgtc
     5821 gctctcgttg ctccggatga ggccgccgat caccgccgtc tcgccgtcgt tcaccatgac
     5881 gcgcgtgtcg gccatcgtcg tgttgatgat gatgccgccc tgcaccgtcg cctgcgacga
     5941 gaggtccgag acctcggggt ggacgtccat cgtgatcttg ttgccgacgt tgatgtgcgg
     6001 cgtcacgctc agcttgatac cgatcgtcgt cagctgggtg actgcgttac ccgcgaaatc
     6061 ctgcacgatg agcggaatct gctggccgac gacgaccgag gcttcgcggt tgttgacagt
     6121 cgtgatccgc ggattcgaga tgatgttcgc cttgttctgc gtctcgagcg cagtcagggt
     6181 cgcctcgatc gagccgaagc ccttcacggt gccgaacttc acggtcccgg ccgggctcgg
     6241 aacctgtccc ggcgtcacgg cgacgccttc atgcgcgccg gcgtcaccga tgtccagatt
     6301 gctgagcgtc caatcgatgc ccagagagcg gctggccgaa cgatccacgt ccacgagccg
     6361 cgccacgatc tcgacctgcg gagtgcgcgt gtcgaggctc cggatcatcg actcggcctg
     6421 cgagagacgg tcgtcgatgt ccgtgaccag gagcgcgttc gtccgcttgt cgacctcgat
     6481 gtggccgcgc ttcgtgagcg tcttctccac ggccttcgac atctcgtccg cgttggcgta
     6541 gctcaccggg atgatccggg tgtagagcgg caggagctcc tcctgtgcgc gctccgccgt
     6601 gcggcgctcg agctcctcct tgcggagcgt ctcgaggttg gagacgacga tgttgttgcc
     6661 gttgtcgacc atgcccaggg cctgcgtgcg aagtaggatg tcgagagcct ggcgccacgg
     6721 cacgttccgc aggcgcaggg tcacggtccc cttcacctct ttgctcgcga cgatgttcct
     6781 gccgctgaac tcggccaggc tacgaaggac cgtctcgacc tcggctccct gcacgtcgat
     6841 cgagatgcgg cggccgtcgc tcggctgcat gttgtcggag ctcgagctcg acggcgacac
     6901 gacaggctcc ggcgccggcg gaggctcggg cgcggcctgc tcgagcgcca gcacgatggt
     6961 gttccccacc gcgctcactc cataagaagt aggcttcgcg agatcgacca cgatccgcga
     7021 gcccgtgtcc ttgccccagc ccatctcggt cgcgcggacc gacttgatgg gcgatttgtc
     7081 ccgccccgcc caggtgcgct cggtgaagcc gagggtcgag cccgggcagt cgagcacgat
     7141 gcgatcgggc gactcgaggg tgaagtcgtg gtagttgatc ggggagtccg cctccacgat
     7201 gacctgggtc cccgttccgg catccttgag cgagatgctc ttgaccgtcg ccgcactcgc
     7261 gtggacggcc gatgccagga ggaggcccat cgccaccgcc gagagaagtc ccgcgcggcg
     7321 acccgtctct ttgctagttt cgagtcgcat tctcgtcacc ctctttcggt ttcagttgga
     7381 tcgaaagcgt ctgcgattcc ccgaaggcgt tctgaaccac ctggatttca ttcttcgtga
     7441 tgcccgagat gtacccgttc acgacggggt ctcccacacg cagcacgaac ccgcgtccgc
     7501 ggctgtcctc cacgagcgcg tacttgtcgg ccgatcccca catgatgccg acgagatgga
     7561 tgtcgccgac gtcgaccaag gcgccgtcac cggacgcgct ctggtacttg ccgtcgagga
     7621 gcgactggaa tggatcgcgg cggccgccgc tcgtatagat gtactgctcg cgcgcgaaga
     7681 ggacgctcgc gtcgagcgac tggggcgtct cttgagcttg cgcggcgacg atgcctgcgg
     7741 cccccgttgc gggcagggca cccgtcgagg ccgcgctgag cggcgtcgtt gaggtcgcgg
     7801 cgcccgtggg tagcgcggtc gcagccgcgg ccggagctcc gggcgcggcc ggcatcggca
     7861 cgcgctcgac ccaatcggga gccggcgggc cgtagacgat cggagcggga acgggcgcgg
     7921 cgggcgcctt gaccggcgtc ggccgcggga ccgcctgcgc ggccggcgac gttgcgacgg
     7981 tcggcgcctt cggcggcgtc aacggacgca tgaagtagaa cacgccggcc ccgatcagga
     8041 ccgccagcac gagcaggagg acgagaaaga tcctgctctg gaagagctta ctttgcggca
     8101 gcgccaggct gcttggcagg ttcattctgg atcccctcgg ccagcgtgta ggcggtggcg
     8161 acgaattcgg cctggacggt gtcgtccagc tcccccttgt caaaaccctt caaccgcagc
     8221 tgggagacgt tcaccagccg cgacagctcg gcgatctcgg cgaggaacga gccggcgtga
     8281 tggaagccgc ccgtgatcgt cacctggacc gggttctccg tgaaatcggg cgtcgggtgc
     8341 ggctcgctcg gcttgaagag cacgaacttc acgcccgact cctgccccgc gatcgtgacc
     8401 ttgcggagga ggctcgccac ttccttctgg gtgggcagga gctcctgcgc cacgctccac
     8461 ttctgattga ggagcgcgaa ctccttcttc agctccggaa ggcggttcac gagctgctgc
     8521 gccttcgtca gctcgttcga ctccttcgtg tagtccgcct tgagggcgct gatctccttc
     8581 gagcgcgctt tgtagttgaa ggggacgtag tcggcgaaga cgtagaagta cacgagcacc
     8641 gccgtcccca gcccgacgag catcaacttc tgcgttttcg gatccttgaa gtccatcgtt
     8701 ctcccctgcg tcaggccggc tggaaggtca tcttggccgt gatcgtgaac ttcaccacgt
     8761 tccgctgatc gatctgaccc ctctccgcga tcaccaggtc gacgtcgccg tagagcggcg
     8821 aggcctcgag gcgggacatg aaatccgcca cgaggaggtt cgagaaggtg accccctcga
     8881 ccttcacctg gtcgggaccg atctcctcgt aattcgtgag ccagaggtga tcgggcacac
     8941 acttgctcag ctcgtcgacc agcttcacgc gccagagccg gttcttgtcg agcgccgcga
     9001 tgacgttgag gcggcgcgtc agctcctcgc gcttctgggt cagctcctgg atcgtctgga
     9061 tctgcgggcg cagcttctcg gcctcctcgc gcgcgtgcgc gatgtcggcc ttgaggcgcg
     9121 ccacctgcat cgcctgaacg atcgagcagg cgacgaggag gcccacgacg ccgaggagcg
     9181 cgaacaccgg aaccatcgcg ccgatgttcg gaagcacgat cttgcgccgg acctggcgtt
     9241 cgtcacgggg caagaggttg atgcgaatca tggctccccc ttatcgcagc gcgaggccga
     9301 tggccacggt cagcatgggc gcgaccgctt cgggcatgtc gccgccgaag aggcccggat
     9361 cgaacgacag gcgctgtacc ggattcacga cggacacggg cacgtgatgg cggccgctca
     9421 ggaagtcggt gagcccggtg atgcgggcgc tgcctcccga gacgaggatc cggtcgatgc
     9481 ggtccgcgtc tcccgccgac ttcaggaagg agatcgcgcg atcgatctgc accgagagat
     9541 cctcggacgc cgtctggacg atcgcggtca cgtcgatgcc gctctcgttc cgtccgcgca
     9601 gcgccgcgag cgcctcgggc tggctcacgt tgtggcggcg ctggatgcct tcgacgaagg
     9661 agttcgaccc cacggagagg tccttcgtga agtgcggcac gccgtcccgg acgatgttca
     9721 gattcgtgat ctcggccccg atgttcacga gcgccaccgt ctcgcccggg aggaactcgt
     9781 agttcgcctc gatcgcgttc tggatggcga aggagtcgac gtcgatgatc gtcggcgtca
     9841 ggccggcctc gcggatgatt tcggcgtggt tttgtaccat gtccttcttc gccgcgacca
     9901 ggaggacctg catcttcttg gcgtcgatgt tgggattgat gatctggaag tcgagcgaga
     9961 cgtcgttgat gtcgtacggg acgtgctgct cggcctccca ctggatcgcc tctttcgcgt
    10021 cttcctcgtt gaggcggtcc atgaggatct tcttcacgat cacggcgcgg cccgacacgg
    10081 ccgtcgccac gctcttggtc cggatctggc gcgactccat cagattcgtg atggcttcgt
    10141 gcaccatctg ccggtccatg atctcgccgt cgacgatcgc ctcggagaga ggctccgaga
    10201 cgccgtagtt gatcaaatgg tccccggagc gatcccgagc gatctccacc accttgatcg
    10261 agctcgagcc gatgtcgaga ccgaccgtgc tcttcttctt cccgaacatg gttcgctccg
    10321 gtctgtgatt gggtttgccg tacccccggc acccgacacc ttgcggggga tccgtcgcgg
    10381 ctggatcgag caagtaccgt gccacgactg gcgaaccctg caacgtaagt catactcgcc
    10441 gaacaggtta cgcggcgagc gccccgcgtc gcgcgctttg cgagcgcggc ccttccactc
    10501 cgtgcattcc gcacggagcg atcgatctcg atccgctgca tcgagcgcgc tggctcgaga
    10561 tcgatcgtcg agggcggtcg cgttgaccga ccttcacccc tctgttagcg tgccgcgcat
    10621 gttccgccac agccgcggcg ccgcttgggt tgccgccctg gtcgcgctct gggtctacgc
    10681 gtccaccctg tcgccgaccg tcgcgtggcg aaacttcggc gaggactccg gcgatctcct
    10741 cgtcgcgtcc gcgaccctcg gcattcccca ccccaccggc tacccgctct acgtgctgct
    10801 cggacgcgtc tcgagccttc taccgatcgg cacgaccgcg ttccggatca atctcgtctc
    10861 ggcgctcgcg ggagccgccg gcgtcttctt catcgcgcgc ctggccgccg agctcgcgcc
    10921 ggccgccggt tccgccgcgc tcgcgatcgc cgcactcgcc gcggcgctcc tctacgcgtt
    10981 ctcgcagggc gcctggagcc agagcgtcgt ggccgaggtc tacaccctga acatcgtgtt
    11041 cctcgccgcg atcctctggg cgctcgcgcg ctccgagcgg agcgggaaga accggtacct
    11101 ggcgctcgcc gcgtatctct tcggtctcgg actctcgaat cacctgctcg tgctcgccgc
    11161 ggcgccggcc atcgcgtggg tggctgtacg tcgcctcgtt tcgaaatcca tcggcgtgac
    11221 gggatgcgtt ctgttgctcg ttcttgtgat ttggggcgtt tcgctcgacg cctatctccc
    11281 gatccgggcg tccctggggc cggagttctc gtggggggtg cccaacaccc cgcaacgcct
    11341 ctggtgggtg ctcacggggg cccagtaccg gggcaacttc ttcagccgcg gcttcgtggc
    11401 tgtaggcagc cacctgttcc cggggcgctg gtggatggac ttcgggccgg ggctgctcgt
    11461 gctcctggga ggggtggccg cgctcctctt cctggcgcgg cgaccgaggc aggatggagc
    11521 ggagggcgcc ccgaacgccg gcgcgtcggc ggtgccggcg ctactcgcgc tcgccgcgtc
    11581 tctcgtcctt ctctcgtcct acacgatccc tgacgacgtc ggttactgga tgccggcggg
    11641 ggccatcctc tgctgcctgg cgggggcggg gctcggcggc gcatgggagc gggttccctt
    11701 cttgggccgc gcggctctag ggatcgcggc cgccgctctc gtgctcctca tggcgagggc
    11761 gaactggccc ctggtcgacg cctcgaatga catgacgccg tacctctacg cgcggcgcaa
    11821 tctcgaggcg gtcgagccga acgcgctcat cgtgagcgac tacgacggcc ggaccttctc
    11881 tctctggttc tacaaggcga ccgagttcaa gcagagccac cccaagctcg cggtcgtcta
    11941 caagagcctt ctcatctggg actggtaccg gtaccacatc gcgaagcgct atcccggcct
    12001 ggtggcgccg tccgggccac cggacccgaa cctcatgctg aatcgcatca tcgcgcggaa
    12061 catcaagaac cgccccgtct acatcgtccg ccccgacacc gcgctcgtca cgctcttcaa
    12121 gaccgagccc gtgggctacc ccccgatgcc gatctaccgc gtccgtctcg tcgagccgtg
    12181 atcgagagcg ttcgagagct cctccgccag cgtggcggga tcctcctcac gctcctgatc
    12241 cttgcggtca cggcgttcca cgtgggcagc atgctccgga ccgagctcac ggtcaccggc
    12301 ggcgggctct cgcttccgct ggatgacagc ttcatctacc tccagtacgc ccgcgcgatc
    12361 gcgcaggggc atcccttcgt ctacacgccg ggaaacgccc ccacgaccgg agcgaccagc
    12421 ctcgggtatc cgctcctcct cctgccgccg catctcctcc gcctcccgcc gtcgatctgt
    12481 atcgcatgga cgctcgcgat cggcgtcgtc ggcttcgtcg cgagcgcgct cctcatcctg
    12541 cgcctcggac gccgtctggg gggctgcgtc ggcggaggcc ttgcgcttgc gctgttcctc
    12601 gcgagcgcac agctcctctg gggatacatg agcggcatgg aggttccgct ctatgcgacg
    12661 gtcctcctcg cgaccctcca cacatatctc cgggagcgca aggaggcgcg cttccccaag
    12721 ctgcgctggt ggctcctcgc gctcgcgctc tcgcggccgg agggagcggt gctctgcttc
    12781 gtgttcggcc tcctggtcct cgccgaccgc caccgcgccg cgcgcgccgg ggcgcctctc
    12841 cgcctggccg agccgatctc catccttccg ttcctgttcg gcctgcttcc gttcctggtc
    12901 aatctcgccg tcgcgggatc gatcgagtcg accagctcgc aggccaagtc gatcctggcc
    12961 gagccctacc gggacgtgcg ggcgagctac atcgagaacg cgccgaaggt ctggagcgac
    13021 atcgccgcga gctacgccgg agcgttcctg ctcaacgagc gcggactccc gttcccgcgg
    13081 atgacgatcc cgttcgcgat cggcgcaggg ctgtacctcg cgttcgcgct ctggccccgg
    13141 gcgcgctcgt gggaccgcgg cctcgccctt tttcccctcg tcggtgcggg catcctcgtc
    13201 aactcgattc ccgtggtctg gcaggtgcac ctgttccgct atcagcaggg gctctatccg
    13261 gtcgtgctcc tcctcgcggc cggggggttc gggcgcctcg ccacgtggtc gtggagcacc
    13321 ctgccgcgcg ccgcgggcgc gctcatcgcg gccatcgccg tcctcgcgcc ggtcgtcgcg
    13381 tggtcccccc tcctcctccg tgcccacgcg gagatcgcgc tcttctacgc ccacaactgc
    13441 gagaacatcc tccatcagca ggtgcgcgtc ggccagtgga tcgaccggaa tctcccgccg
    13501 aacgccatcg tcggactgaa cgacgccggg gccatcgcgt actacggcaa ccgctcgacg
    13561 ctcgacctgg tcggactcac gacggccggc ttcgcgggcg tgtatcgctc ggggctcggc
    13621 tgtctgttcg agcacctgcg tcgtcttcca gcggaccggc tcccgaccta cttcgccatc
    13681 tacccaggat ggtttcccta ctgggaggag agcgggatcc tcggacccga ggcctaccgc
    13741 gcgcacctcg gcatcaacac gatcaccgga ggcacggaca tggtcgtcta ccccgcgtcc
    13801 tggatcgacg tggagccgac cgatcacccg gttcttccgc accgggagat cgagggcaag
    13861 cgcccggtcg actcgatcga cctggcgtgg ctcgaggatg aatcacgcca cgagtggagc
    13921 tcggatcccg aagcgaccga tgttctccgg cgctacgcct acgccgatgt ccggaaccgc
    13981 cccatcacgg acgcgggacg gatcgtccgg aagagcgagc gctttcgcgc gtccgtcacg
    14041 ccgggacggg atctcacgct cgtgatgcgc accgacgcct ggtacccaac gcacctcgac
    14101 gtccaggtgg acggagctcc cgctggaacc tggagcatcg cgaggtcgga gtcgacctgg
    14161 gtcgagccca ccttcaccat ccccgcccgc ctcatccagc acccccgctc cgaaattggc
    14221 ctcacgcgcc aggtaaccac ggacgtcgag gcccagcgcg actacgcccc cttccactac
    14281 tggctttatc aatgacggag cgatcgggga aagcccggag cgtcaccgat gcttcggtag
    14341 ggccgtgcac ggactgaccg gatggcgcct ctcgagcttt gcgcggagcg caaagcgaag
    14401 agccgcgccc ggaggt
//
LOCUS       DASTKC010000075         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1236179, whole genome shotgun sequence.
ACCESSION   DASTKC010000075 DASTKC010000000
VERSION     DASTKC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236179"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2650
                     /locus_tag="VFR25_03360"
     CDS             <1..2650
                     /locus_tag="VFR25_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9940118.1"
                     /translation="WLADARRRGMVATEAAIDSRMKQSNYFKKGGKVDEAMFQRFKKS
                     ETSNYPALHEEIQNAILLEEYVRWMERRFGPRDAELRKEFETRSATASIRYMLMGPDA
                     VSLDPEATPAQIRAYYDAHPDEFESADEAHIQYIRIPIGADASVPDSAKDTAAKVAFQ
                     VASDVIAAIRTGAPAETAAKPYGGIQDPGWTKLGEPIRGLGRSEALTAAIKSVEPPAW
                     IRDPIRIGPMYVVVNLVDRHRARRLPFHEVVYYAKRKADFEVRDQMLDSLAREDVRLH
                     PESYYAPKIEVSIVARGLNTFEAGPPPTPKEIDRRLEKLKDDLHLPDSTRAYLDSTRA
                     SIPALILEERQVKSGFHVMKDAAGKLSKKESASRVASRYGGVLSVVRTHRGQPPENAS
                     LLEGKLLDSLYTMRAGAVIGPRVKDDSVYVVRLETIEGTYVPPFAEIRDDAREAAEAK
                     QRADVAKDAEAWFSSRGGTYRTPSKYILDVVFFAKKKPEQIAVPDDSIEAYWRQHPLE
                     FTEPAKAKVRHILFRAQDPSALGPARQKALAVRKRIVDGEDFGAVARGASEDQGSAAR
                     GGDLGELTRGAVPKEFGDAMMTLAIGELSEPIETQFGYHILKVDSRTPERLRPLDECR
                     TEIRGVLADQIADSLARIPARILADRASRVGYRAADSLAAPYGGWKRKGPVGPDGEIE
                     GVAKVPFIARVVASLADSGVAAEPVELPEGDVVIRKVREETPRQARFEEVRDKVIADY
                     QEERRRAASDSLNVRLRAALKEGANPETLFVAFGGLRPSKPFGRQGPIPDFQKDAVLG
                     RDSTYLARVFASKPKTALPPITGGSGTLYGVVESVTVPPASDFARQRDALLRQVLDER
                     IDAWTGRLRTKAPIRMNRKDLQALLS"
     gene            2714..3400
                     /gene="radC"
                     /locus_tag="VFR25_03365"
     CDS             2714..3400
                     /gene="radC"
                     /locus_tag="VFR25_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET9940119.1"
                     /translation="MDRESGVLMHDVEESERPRERLFELGPGALSDADLVALVFGSGS
                     GGESVLDVAARVTRSIHLRRLHQTAVEDLLAVKGMGPARAAQLLAAAEIGRRLWPDAD
                     PIPLVRGPETIFELTADIRRSNREHFVGFYLNSRNQVLRREIISIGSLNASIVHPREV
                     FIPALAVSAASLILAHNHPSGDPSPSEEDLAITRRIAESGRLLGIELLDHVVVAREAY
                     TSFKERRLLR"
     gene            3397..4410
                     /locus_tag="VFR25_03370"
     CDS             3397..4410
                     /locus_tag="VFR25_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940120.1"
                     /translation="MRARWSALVLAIVLAGCAGSRPEGEPEAKIDPNASPAALIALSD
                     SALAVAEPVLARRALERAASLAPKSAAVRLGYGRFYTAIQRYKDAKTEFERAETLDPS
                     SPEPAYGLGLAYLRAGQQDLAFASLSRALRIDPMHVGARTAIRPMLEDRYRRAGVPPE
                     YASIPEQSSVSRGELGVILAVEAGLDPDRVSFHSDAPYRVDWPAIDKAWGSRWLRASV
                     ARGWIAPFADRDLHLDDPLTRGNLAVLLGRIEASSPRAPADTARAAHAPAAAFPDLGG
                     RHYLGHAAAEAVRLGLPTRDGGRFDAEALATGAETMIAARGLARRMGALPVVSIDPPG
                     LVK"
BASE COUNT          704 a         1521 c         1556 g          650 t
ORIGIN      
        1 gtggctggcc gatgcccgcc ggcgcgggat ggtcgcgacc gaagcggcta tcgactcccg
       61 gatgaagcag agcaactact tcaagaaggg gggcaaggtc gacgaggcga tgttccagcg
      121 cttcaagaag tcggagacgt cgaactatcc ggcgctccac gaggagatcc agaacgcgat
      181 cctcctcgag gagtacgtcc gctggatgga gcgccggttc ggcccgcgcg acgccgagct
      241 tcgaaaggag ttcgagacgc gcagcgcgac ggcgtccatc cgctacatgc tcatggggcc
      301 ggacgcggtg tcgctcgatc ccgaggcgac cccggcacag atccgcgcct actacgacgc
      361 gcatccggac gaattcgaga gcgccgacga ggcgcacatc cagtacatcc gcattccgat
      421 cggagcggac gcatcggtgc ccgactcggc caaggacacc gcggccaaag ttgcgttcca
      481 agtcgcgtcc gacgtgatcg ccgcgatccg caccggggcc cctgccgaga cggcggcgaa
      541 gccgtacggc gggatccagg atccgggctg gaccaagctc ggcgaaccca tccggggcct
      601 gggccgctcc gaggccctga cggccgcgat caagagcgtc gagcctcctg cctggatccg
      661 cgatccgatc cggatcgggc ccatgtacgt ggtcgtgaac ctcgtggatc gccaccgcgc
      721 ccggcggctt ccgttccatg aggtggtcta ctacgcaaag cgcaaagccg atttcgaagt
      781 gcgcgaccag atgctcgatt cgctcgcgcg cgaggacgtg cggctccatc cggagtcgta
      841 ctatgccccc aagatcgagg tctcgatcgt ggcgcgcggc ctcaacacgt tcgaagccgg
      901 accgcctccg acgccgaagg agatcgaccg gcgcctcgag aagctgaagg acgacctcca
      961 tctccccgat tcgacgcgcg cctacctgga ctcgacgcgc gccagcatcc cggcgctcat
     1021 cctcgaggag cggcaggtca agtcgggctt ccacgtcatg aaggacgcag ccgggaagct
     1081 ctccaagaag gagtcggcct cccgggtggc gtcgcgctac ggcggggtcc tgagtgtcgt
     1141 ccgcacgcat cgcgggcagc cgcccgagaa tgcatccctc ctcgagggga agctcctcga
     1201 cagcctctac accatgcgcg ccggtgcggt catcggcccg cgtgtgaagg acgactccgt
     1261 gtacgtggtc cgcctcgaga cgatcgaggg cacgtacgtg cccccgttcg cggagatccg
     1321 ggacgacgcg cgcgaggcgg cggaagcgaa gcagagggcc gacgtcgcca aggacgccga
     1381 ggcctggttc agctcgcgcg gaggcaccta tcggacgccg agcaagtaca tcctggacgt
     1441 cgtcttcttc gccaagaaga aacccgagca gattgcggtg ccggacgact cgatcgaagc
     1501 ttattggcgc cagcatccgc tcgagttcac cgagccggcc aaggcgaagg tccgccacat
     1561 cctcttccgc gcgcaggacc cgtccgccct gggtcccgcg cgtcagaagg cgctcgcggt
     1621 gcggaagcgc atcgtcgacg gggaggactt cggcgccgtc gcgcgcggtg cgtcggaaga
     1681 ccaagggtcc gcggcccgag gcggcgacct cggcgagctc acgcgaggcg cggtcccgaa
     1741 ggagttcggg gacgcaatga tgaccctcgc catcggcgag ctgagcgagc cgatcgagac
     1801 ccagttcggc taccacatcc tcaaggtgga ctcgcggacg ccggagcgcc tgcgtcccct
     1861 cgacgagtgc aggaccgaga tccggggcgt cctcgccgac cagatcgcgg attcgctcgc
     1921 gcgcattccg gcccgcatcc tcgcagaccg ggccagccgc gtgggttatc gcgccgccga
     1981 ctcgctggcc gcgccgtacg gtggatggaa gcgaaagggg ccggtcgggc cggacgggga
     2041 gatcgagggg gtcgccaagg ttccgttcat cgcgcgcgtg gtcgcgtcgc tcgcggacag
     2101 cggcgtggcg gccgagcccg tcgagcttcc ggagggggac gtcgtgatcc gcaaggtccg
     2161 ggaggaaacg ccgcggcaag cgcggttcga agaggtcagg gacaaggtga tcgcggacta
     2221 tcaagaggag cgtcgacgcg cggcttcgga ttcgctgaac gtgcgcttgc gagccgcgct
     2281 caaggagggc gcgaatcccg agacgctctt tgtcgcattc ggcggccttc gcccgagcaa
     2341 gcccttcgga cggcagggcc cgattcccga cttccagaag gacgcggtcc tcgggcgcga
     2401 ttcgacctat ctcgcccggg tcttcgcgtc gaagcctaag accgcgctgc ccccgatcac
     2461 gggggggagc ggcacgctct atggcgtggt cgagagcgtc acggtcccgc cggcgagcga
     2521 cttcgcgcga cagcgcgacg cgctccttcg ccaggtgctg gacgagcgga tcgacgcgtg
     2581 gaccggacgg ctccggacca aggcgcccat tcggatgaat cggaaggacc tgcaggccct
     2641 cctcagctga ggtaccatcg agactcctcg cgcttcatca ccccgcgcgt ccccgctccc
     2701 ggaggattcg agcatggatc gggagtcggg cgtcttgatg cacgatgtgg aggaatcgga
     2761 gcggccgcgt gagcgcctgt tcgagctggg accgggcgct ctgtctgacg cggatctcgt
     2821 cgcactcgtc ttcggctcgg gcagcggagg cgagagcgtg ctggacgtcg cggcgcgcgt
     2881 cacgcggtcg atccatctga gaaggctcca tcagaccgcg gtggaggacc tcctcgcggt
     2941 caaggggatg gggccggccc gcgcggcgca gctcctcgcc gccgcggaga tcgggcgaag
     3001 gctctggccc gacgccgatc cgatcccgct cgtgcgcgga ccggagacga tcttcgagct
     3061 gaccgcggat atccggcgct ccaatcggga gcatttcgtg ggcttctacc tgaattcgcg
     3121 gaaccaggtg ctccgccggg agatcatctc catcggaagc ttgaacgcga gcatcgtcca
     3181 cccccgcgag gtcttcatcc ccgcgctcgc tgtctccgcg gcgagcctga tcctcgcgca
     3241 caaccatccg tccggcgatc cgtcgcccag cgaggaagac ctggccatca cccgccgcat
     3301 cgccgaatcg ggacgcctcc tcgggatcga gctcctggac cacgtcgtgg tcgcgcggga
     3361 ggcgtacacg agtttcaagg agcggaggct tcttcgatga gggcccggtg gagcgccttg
     3421 gtgctcgcga tcgtcctcgc gggatgcgcg ggctcgcgtc ccgaggggga gcctgaggcg
     3481 aagatcgacc cgaacgcgtc ccccgcggcg ctcatcgcgc tctccgattc cgcgctggcg
     3541 gtggccgagc ccgtgctcgc ccgacgcgcg ctcgagcgcg cggcgtccct cgcgccgaag
     3601 agcgcggcgg tgcgccttgg ctatggacgc ttctacacgg cgatccagcg atacaaggat
     3661 gcgaaaaccg agttcgagcg cgcggagacg ctcgatccgt cgagcccgga gccggcgtat
     3721 ggtctgggcc tcgcgtacct gagagcgggg caacaggacc tggcgttcgc gtcgctctcg
     3781 cgcgcgcttc ggatcgaccc catgcacgta ggagcgcgca cggcgatccg cccgatgctc
     3841 gaggatcgct accggcgcgc gggcgtgcct ccggagtacg cctcgatccc ggagcagtcg
     3901 agcgtttcgc gtggagagct cggcgtgatc ctggccgtgg aggcggggct cgatccggac
     3961 cgcgtgtcgt tccactcgga cgcgccctac cgcgtggatt ggccggcgat cgacaaggcg
     4021 tggggctcgc gatggctgcg cgcgtcggtg gcgcgcggat ggatcgcccc cttcgccgat
     4081 cgcgacctcc atctcgacga cccgctcacc cggggcaacc tcgccgtgct gctcggccgg
     4141 atcgaggcgt cctctccgcg cgcccccgcg gacacggcac gggcggcgca tgcgccggca
     4201 gcggccttcc cggatctcgg cggccgccac tacctcgggc acgccgcagc cgaggcggtg
     4261 cgcctcggtc ttccgacgcg cgatggaggg cgcttcgacg cggaggcgct cgcgaccggg
     4321 gccgagacca tgatcgccgc gcgcgggctc gcgcgccgca tgggggcgct gccggtcgta
     4381 tcgatcgacc ccccgggtct ggtaaaatga tcgtcgtgaa gcgcattccg g
//
LOCUS       DASTKC010000076        10760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1472111, whole genome shotgun sequence.
ACCESSION   DASTKC010000076 DASTKC010000000
VERSION     DASTKC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472111"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1121)
                     /gene="dapB"
                     /locus_tag="VFR25_03375"
     CDS             complement(366..1121)
                     /gene="dapB"
                     /locus_tag="VFR25_03375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9940121.1"
                     /translation="MAEVPILLHGATGRMGRAVRDCLPEFPDVKLVACVAPREQAPGP
                     APGPLWLTPEELTTHRRREALPDDLVVIDFSLAAGTQALLKTLALWPRALVCGTTGLS
                     QESERMLEMLAGRVPVFRASNLSLGNALVETLLRALPRHVIRTYTADIVEHHHAGKKD
                     APSGTAITLARVLGEVGAVGQEGTEIRIQSIRSGTVPGTHRVILGGAGETIEIVHTVT
                     DRGVFARGALRAAGFIHGQVPGRYTMDDMLRDV"
     gene            complement(1128..2024)
                     /gene="dapA"
                     /locus_tag="VFR25_03380"
     CDS             complement(1128..2024)
                     /gene="dapA"
                     /locus_tag="VFR25_03380"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9940122.1"
                     /translation="MAAIFEGLGVALVTPYRKGSVDTDAAAKLAERLIERGVRAIYPC
                     GCTGEATSLTREERTRLIRAVVEAVRGKGAVIAGTGTANTDETIELSKEAIQLGADAV
                     MVITPYSVRPTQEGLIAHYRAVAAAIDRPMVLYNVPARTGSSIAPETVAKLAENPRIA
                     AIKEASGSVDQTSAIRARCDITVLSGDDSLYLALLAVGARGLVSVAGHLVPAELVELE
                     HHFHAGRIGEAEKVHRKLTPLFKALFLETSPAPVKHALARLGLIEGEIRLPLVPVRPE
                     TGKAVEAAMDAVGLAESARKSA"
     gene            complement(2026..3084)
                     /locus_tag="VFR25_03385"
     CDS             complement(2026..3084)
                     /locus_tag="VFR25_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9940123.1"
                     /translation="MLNRRSLAVLACVVSAGAMAGCARSVFPPGGPLDTIPPKIIAVT
                     PADSTVQVARDAGVELMFSESMDRATVRDGIRVYPPPGRPRLDWSGRRARVTWDEPLA
                     ESTTYVLVVSGQARDERGVPMGRPITVRFSTGGSLDSGRISGVLRTHTLVKRGVPILA
                     FDESLGERPDSSDALPSYATETDTAGVYELTALPVGRGFRILAWFDINTNGSIEPGTD
                     LVSFYPDPVRLTRERARADSINIVAIDRRAPAVLNGRIASRDSTSKYRVEAQDTEDSS
                     LVIRVERTGPGPFTLRVSAGTYRLRVASLPPPDSPEEPVWITREEPIVTKPEEEYGPF
                     EFAFGTVEETPPPPEGGE"
     gene            complement(3084..4115)
                     /gene="argF"
                     /locus_tag="VFR25_03390"
     CDS             complement(3084..4115)
                     /gene="argF"
                     /locus_tag="VFR25_03390"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET9940124.1"
                     /translation="MKRDLLSFADLTRADVDRLFAVAKRLKADLRAGRRHQELAQQTL
                     ALIFHKPSLRTRLSFEVAMTQLGGSSVFITDREIGIGSREPVQDVARVLSGYVDGIMI
                     RTFDHQLAVGLARHATVPVINGLTDWLHPCQILADLFTLVERGLDPDKLIVTYIGDGN
                     NVANSWVEAAGLFGLTVRIACPDGYEPDQNLVQQIDSSGRGRVEIHHDPAVAATGADV
                     LYTDVWASMGQEAEREKRLPIFRNFQINADLIRRARRGAVVLHCLPAHRGEEITEEAM
                     EGPQSAVFDQAENRLHLQKAVVYELLGPAGKRSAKGAGGSKGATSRKAASRVPRLGRS
                     VAGRPRRGR"
     gene            complement(4156..5073)
                     /locus_tag="VFR25_03395"
     CDS             complement(4156..5073)
                     /locus_tag="VFR25_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9940125.1"
                     /translation="MERWIAEFLSYLKENRRCSPLTERAYGEEMRRFAEFATGRLGKA
                     PRVPQDLTPELIAAFAAARSLERTAKKRPIASRTLARSVAAVRSFLRYVERRGHSTTA
                     ARTAMPRVSTPDTLPASVPEASLNALLDELASKVPADDVRGLRGLAAAECLYGAGLRV
                     GELAGLTRGRLDERRRLVRVLGKGSKERVVPISERALHALKRFWTARGKAPEAGDALL
                     AGPSGRGVTSRTLERDIESILGRLGPSAPTHAHALRHSFATHLLDRGADLRAVQELLG
                     HSNLGTTQIYTHVTRRRLKAAYARAHPRA"
     gene            complement(5054..7534)
                     /gene="topA"
                     /locus_tag="VFR25_03400"
     CDS             complement(5054..7534)
                     /gene="topA"
                     /locus_tag="VFR25_03400"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9940126.1"
                     /translation="MKSKAKSVKQAEAAEAVETAAEAKPRPATRKKVAAAAKAAPAPA
                     VEKVPAGVKGATKKKVAARKKASAKREAPAAGGRRRNLVIVESPAKARTIAKYLGPGY
                     EVKASNGHVRDLPKSKLGVDVEKGFEPSYVLIKGKGKILKDLKASARRAATVYLAPDP
                     DREGEAIAWHLAETLGDDADDIRRLAFYEITKRGVTEALEHPRTIDMAKVQAQQARRV
                     LDRLVGYKVSPFLWRTIRYGLSAGRVQSVALRLICEREEEIRKFVTREYWTIDADLVT
                     PAGDIFRARVQKKAGEKFALENEKQARADADALARESFAVTQVRTQEKKRNPVPPFIT
                     STLQQESFRRHRYSGQKTMVIAQQLYEGVEVGEGATGLITYMRTDSTRVSPDAIAEVR
                     AFIQEQYGDEYLPAEVKVYRSRETSQDAHEAVRPTSVARTPASIKGYLDPDQYKIYEL
                     IWQRFVASQMNPALVLTTTAEITAGPYLLRASGSRVKFDGFARAYATALIEETPEGQA
                     SKLPALAEGDKLKMKEILPEQHFTEPPPNYTEASLVKVLEEKGIGRPSTYATIVGTIL
                     TRDYVTRDRGKLLPTELGMTVWKLLEQMFADVFDVAFTAKLESHLDRIESGKDPWVDV
                     VTEFYDPFQSDLAKAESQQEKVRASLVQETDVACPKCGSKMVKKFGRSGPFLACPRYP
                     ECKATMPLEEEGIPAEAPTQKCPQCGSAMRVRTGRFGKFLACSTYPECKGTRPFTLQI
                     PCPKCVQGELVERRTRRGKPFFGCSRYPECDFGIWDRPVKQKCPSCDSPILVKKSSKA
                     KGEYLQCPKCKIQVESESTEALSGTLDR"
     gene            complement(7540..8013)
                     /locus_tag="VFR25_03405"
     CDS             complement(7540..8013)
                     /locus_tag="VFR25_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940127.1"
                     /translation="MDESVREMVQVIVERLKAFVDGDEDALLELSRVLDSGRFDASVV
                     GEAFEMIVRALEPYAREDFGDGSAKERPNVRVPSGPERALLLANPAYAYLFRLLEAGK
                     VSPEQFEEILSRARDMGPSLHDEAQARELAIEVLIRWFDDEHGVTFDPAPTAGAH"
     gene            complement(8028..>10760)
                     /gene="secA"
                     /locus_tag="VFR25_03410"
     CDS             complement(8028..>10760)
                     /gene="secA"
                     /locus_tag="VFR25_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET9940128.1"
                     /translation="HVVTVNEYLAQRDADWMAPVYRFLGLTVGVIKSNQPTEEKRAAY
                     AADITYGTNNEFGFDYLRDNMARHRDHRVQRGHNYAIVDEVDSVLIDEARTPLIISGP
                     VDHSLQQYDEMKPDVDRVVRSQGQITNRILAEAETMLQDPEKEYEAGVKLLQVKRGAP
                     KNKRFLKLVAEQPGLKRLIQKVELDFIRDKRLGEVDEDLLYSIDEKSHSVDLSEQGRT
                     LLSQRDPNRFLLPDLAVELGAIDEDEAISPADKVQKKRELERQHAIRSERVHDILQLL
                     KAYSLFEKDVEYVVQDGKILIVDEFTGRLMPGRRYSDGLHQAIEAKENVKVEGETQTL
                     ATITIQNYFRLYDKLAGMTGTAETEASEFWHTYKRDVVVIPTNQPVRREDSNDVIFKT
                     RREKYNALVDEIASMHAQGIPVLVGTISVEASETLSRLLKRKGVPHNVLNAKYHQQEA
                     EIVANAGRRGAVTIATNMAGRGTDIKLEKGVIRCDRGCYVNQQSKLDNGVWRNVGRCK
                     EEMTCGLHILGTERHESRRIDRQLRGRSGRQGDPGFSRFYLSLEDDLMRLFGSDRIAG
                     IMERLGVQEGEVIEHGLVTRAIERAQKRVEAHNFDIRKHLLEYDDVMNKQRTVIYAQR
                     LRALEEADLKETMLEMMDEMVSERVHTYLGSHERYDEEDLKRVLNDVSQLLLRPVKLP
                     DQGDRLPSPDEVEEEIQEIFHKAYEEKEQEMSAPILRELERVVYLEVIDEHWMDHLRE
                     MDHMREGIGLRAYGQRDPLLEYKKEAFEMFEELTRSIREETVRNLFRAALVMEPVPGR
                     GLPSLAVGPAGASGAPPEMPRILPGRPAAKSAPRQQEVHAEVTAFGTMAEPQEDSGAS
                     QRPGGRSAAPPPRPGAAPPGAKQAPVVSTEPKVGRNDPCPCGSGKKYKKCHGAN"
BASE COUNT         1603 a         3690 c         3573 g         1894 t
ORIGIN      
        1 gcttggctgt gacgtcaaga ctcgttaaac gtggtctttc gatcgtcgcc actcgacggg
       61 cgaagtgctc ccggacatcg cagacccaaa tcgcgatcct cttatcaatg acaatcggag
      121 cgaccccgaa aaaggcggga atgcctcgtg gatccatcct aagagatgaa tacatcagaa
      181 ctgcaaagcg tggttcagga gcttgtacat aactggggag acagcgatgt tcccactggg
      241 gcggcgagta aaggcaactt cccacaaagg taagagcgcc gaccgacagg atgagagcca
      301 aggtccagcg ccttgatgac cttttctctt cttcagggct cacaatcgaa tgcaagccac
      361 acacctcaca cgtcgcggag catgtcgtcc attgtgtagc gtccgggcac ctgcccgtga
      421 atgaaccctg ctgcgcgcag cgcgccacgg gcgaagaccc cgcggtccgt cacagtgtgg
      481 acgatctcga tagtctcccc cgcacccccg agaatcacgc gatgcgttcc ggggacggta
      541 cccgagcgga tggattggat ccggatctcg gtcccttcct gccccacagc tcccacctcg
      601 cccagcacgc gggccagcgt gatagcggtg ccgctcggag cgtccttctt ccccgcgtgg
      661 tgatgctcga cgatgtcagc cgtataggtg cggatgacgt ggcgcgggag ggcgcgcagc
      721 agggtctcga cgagggcgtt gcctagcgag aggttcgacg cgcggaacac gggcacgcgt
      781 cccgccagca tctcgagcat gcgctccgat tcctgggaca gcccggtcgt cccgcacacg
      841 agcgcgcgcg gccagagcgc gagggtcttc agcaaagcct gcgtcccggc tgccaagctg
      901 aagtcgatca ccacgagatc gtctgggagc gcctcgcgtc ggcggtgggt cgtcagttcc
      961 tcgggggtga gccacagtgg tcccggcgcc gggcccggcg cctgctcccg cggagccacg
     1021 cacgccacca gcttcacatc cgggaactcc ggcaggcagt cgcgcaccgc gcggcccatg
     1081 cgtccggtgg cgccgtggag gaggatcggg acttcggcca tgggtcgcta cgcggacttc
     1141 cgcgcggact ccgcgagccc caccgcgtcc atcgcggcct cgaccgcctt ccctgtctcg
     1201 ggacgcacag gcaccagcgg gagccgaatc tcaccctcga tcaagccgag gcgcgcgagc
     1261 gcatgcttca cgggcgcggg gctggtctcg aggaagagcg ctttgaacag aggcgtgagc
     1321 ttccgatgca ccttctccgc ctcgccgatg cgtcccgcgt ggaagtgatg ctccagctcg
     1381 acgagctcgg ccggcacgag atgcccggcc acggacacca gaccgcgagc gccgacggcc
     1441 agcaacgcca ggtacagcga gtcgtccccc gagagcacgg tgatgtcgca cctcgcgcgg
     1501 atggcgctcg tttgatccac cgagccactc gcctccttga tcgccgcgat gcgtggattc
     1561 tccgcgagct tggccaccgt ttcgggggcg atcgaagatc ctgttcgcgc aggcacgttg
     1621 tacaagacca tgggccggtc gatcgcggcc gccaccgcgc ggtagtgagc gatgagccct
     1681 tcctgcgtcg ggcggaccga atagggtgtg atcaccatca ccgcgtccgc gccgagctgg
     1741 atcgcttcct tcgacaactc gatcgtctcg tccgtgttgg cggtgccggt tccggcgatc
     1801 acggcgccct tgccgcgcac ggcctcgacg acggcgcgaa tcaaccgggt gcgctcctcg
     1861 cgcgtgagcg aggtcgcctc gcccgtgcag ccgcacggat agatcgcacg cacgccgcgc
     1921 tcgatcagac gctcggcgag cttcgccgcc gcgtccgtgt cgaccgagcc cttccgatac
     1981 ggcgtaacga gtgctacgcc cagaccttca aagatcgcag ccatgtcact cccctccctc
     2041 gggcggcggc ggggtctcct ccaccgtgcc gaacgcgaac tcgaagggac cgtactcctc
     2101 ttcgggcttg gtcacgatcg gctcctcgcg cgtgatccag accggctcct cggggctgtc
     2161 cggaggcggc aggctcgcca cgcggaggcg ataggtcccc gccgacactc ggagcgtgaa
     2221 tggccccggc cccgttcgtt ccacccggat cacgagagac gaatcctcgg tatcctgcgc
     2281 ttcgacgcgg tacttcgagg tcgaatcgcg cgacgcgatc cgtccgttca ggaccgccgg
     2341 ggcgcgccgg tcgatcgcga cgatgttgat actatccgct cgcgctcgct cccgcgtcaa
     2401 gcggaccggg tccggataga acgagacgag gtcggtgccg ggctcgatcg agccgttcgt
     2461 gttgatgtcg aaccaggcga ggatccggaa cccgcgcccc accggaaggg cggtcagctc
     2521 gtagacgccg gcggtgtcgg tctcggtggc gtaggacggg agggcgtccg agctgtcggg
     2581 gcgctcgccg agcgattcgt cgaaggcgag gatgggaacg ccgcgcttca cgagggtgtg
     2641 cgtgcggagc acgcccgaga ttcgcccgct gtcgaggctc ccgccggtgc tgaaccgcac
     2701 ggtgatcggc cggcccatgg gaactccgcg ctcgtcccgc gcctggccgc tcacgacgag
     2761 cacgtacgtc gtgctctcgg cgagcggctc gtcccaggtc acgcgcgccc gccggccgct
     2821 ccagtcgagc ctcgggcgtc ctgggggagg atagacgcga atcccgtccc gcacggttgc
     2881 gcggtccatc gactccgaga acatcagctc caccccggcg tcgcgcgcga cctgcacggt
     2941 gctgtccgcg ggagtcacgg cgatgatctt cggaggaatg gtgtcgagcg gccctcccgg
     3001 cggaaagacc gaccgcgcgc agcccgccat ggcccccgcg gagacaacgc acgcgaggac
     3061 ggcgagggag cggcggttga gcatcagcgc ccgcggcgtg ggcggccggc gacgctccgt
     3121 ccgaggcgcg gcacgcggga agcggccttg cgggacgtgg cccccttgga ccccccggct
     3181 cccttcgccg aacgcttccc cgcagggcca agcagctcgt agaccaccgc cttctggaga
     3241 tggagccggt tctccgcctg gtcgaacacc gccgactgcg gcccctccat cgcctcctcg
     3301 gtgatctcct cgccccgatg cgcgggcagg cagtggagca ccacggcgcc gcggcgcgcg
     3361 cgccggatga gatccgcgtt gatctggaag ttacggaaga tcgggagccg cttctcgcgc
     3421 tccgcctcct gccccatgct cgcccagaca tcggtgtaga gcacgtccgc gccggtggcc
     3481 gcgaccgcgg ggtcgtgatg gatctcgacc ctcccgcgtc ccgacgagtc gatctgctgg
     3541 accaggttct gatcgggctc gtaaccatcg gggcacgcga ttcggaccgt gagtccaaag
     3601 agaccggcgg cctcgaccca ggagttggcc acgttgttcc cgtctccgat gtacgtcacg
     3661 atgagcttgt cgggatcgag gccccgctcg accagcgtga agaggtccgc caggatctgg
     3721 cagggatgga gccagtccgt gaggccgttg atgaccggca ccgtggcgtg ccgcgcgaga
     3781 ccgacggcga gctggtgatc gaacgtgcgg atcatgatcc cgtcgacgta gcccgagagc
     3841 acgcgcgcca cgtcctgcac cggctcgcgc gatccgatcc cgatctcgcg atccgtgatg
     3901 aacacggagc tcccgccgag ctgggtcatc gcgacctcga aggagaggcg cgtcctgagg
     3961 ctcggcttgt ggaagatcag agcgagggtc tgctgagcca attcctggtg ccggcggccc
     4021 gcgcggaggt cggccttgag acgcttggcc accgcgaaca ggcggtcgac gtcggcgcgc
     4081 gtgagatcgg cgaacgagag aaggtcccgc ttcatgcgtc ggcgctcccg gttgtggagg
     4141 aaatgaggtc cggcgtcacg cgcgcggatg cgcccgcgcg taggccgcct tgaggcggcg
     4201 ccgggtcacg tgcgtgtaga tctgcgtcgt gccgagattg ctgtggccga gcagctcctg
     4261 gacggcgcgc aggtcggcgc cgcggtccag gagatgcgtg gcgaagctgt gccgcagtgc
     4321 gtgggcgtgg gtcggagcgc tcggtccgag acggccgagg atcgactcga tgtcccgctc
     4381 gagggtcctg gacgtgacgc cccggcccga agggccggca aggagcgcgt cccccgcttc
     4441 cggcgccttg ccccgggcgg tccagaatcg cttcaaggca tgcagcgcgc gctcgctgat
     4501 cggaaccaca cgctccttgc tgcccttccc gagcacgcgg acgaggcggc ggcgctcgtc
     4561 cagcctgccg cgagtgagcc ctgccagctc gcccacgcga agccccgcgc catacagaca
     4621 ttcggcggct gcgaggccgc gcaggccgcg aacgtcgtcg gccgggacct tcgaagccag
     4681 ctcgtcaagg agggcattca aagaagcctc ggggacggag gcgggaagcg tgtcgggggt
     4741 cgagacgcgc ggcatcgccg ttcgcgccgc ggtggtcgag tggccgcgtc gctccacgta
     4801 gcggaggaag gaccggacgg ccgccaccga gcgcgcgagc gtgcgcgagg cgatcggccg
     4861 cttcttcgcc gtgcgctcga gcgagcgcgc cgcggcgaac gcagcgatca gctcgggcgt
     4921 cagatcttgt ggaacgcggg gggctttgcc caggcggcca gtggcgaatt cagcaaatcg
     4981 gcgcatctcc tcgccgtagg cccgctccgt gagcggcgag cagcgtcgat tctctttcag
     5041 atagctgagg aactcagcga tccagcgttc cactcagcgc ttccgtgctc tccgattcga
     5101 cttggatctt gcacttcgga cactggagat actcgccctt cgccttgctc gacttcttca
     5161 cgaggatcgg cgagtcgcag ctcgggcact tctgcttcac tggccgatcc cagatgccga
     5221 agtcgcactc gggatagcgg ctgcagccga agaagggctt cccgcgccgc gtcctgcgct
     5281 cgaccagctc cccctgcacg cacttggggc atgggatctg gagcgtgaag gggcgggtcc
     5341 ccttgcactc gggataggtg gagcacgcca ggaacttgcc gaaccgtccg gtgcggacgc
     5401 gcatggcgct cccgcactgc gggcacttct gggtcggagc ctcggcgggg atgccctcct
     5461 cctcgagcgg catcgtcgcc ttgcactcgg gataccgcgg acaggccagg aacggcccgc
     5521 tccgcccgaa tttcttcacc atcttcgagc cgcacttcgg gcaggccacg tccgtctcct
     5581 ggacgagcga ggcgcgcacc ttctcctgct gcgattcggc cttggcgaga tcgctctgga
     5641 agggatcgta gaactcggtg accacatcga cccacggatc cttgcccgac tcgatccggt
     5701 ccagatgcga ctcgagcttc gccgtgaacg cgacgtcgaa gacgtcggcg aacatctgct
     5761 cgaggagctt ccagaccgtc atgccgagct cggtcgggag gagcttcccg cgatcgcggg
     5821 tcacgtagtc gcgcgtgagg atcgtcccca cgatcgtggc gtacgtgctc gggcggccga
     5881 tccccttctc ctcgagcacc ttcacgaggg acgcttccgt gtagttggga ggcggctcgg
     5941 tgaagtgctg ctcggggagg atctccttca tcttcagctt gtccccttca gccaaggcag
     6001 gcagcttgct cgcctggccc tcgggagtct cctcgatgag cgcggtcgcg taggcgcggg
     6061 cgaagccgtc gaacttgacc cggctccccg acgcgcggag gagatacggc cccgccgtga
     6121 tctcggcggt ggtcgtcagc accagcgccg gattcatctg cgacgccacg aagcgctgcc
     6181 agatgagctc gtagatcttg tactggtccg gatccaggta gcccttgatc gacgccggcg
     6241 tgcgcgcgac cgacgtcggc cggaccgcct cgtgggcgtc ctgcgacgtc tcgcgcgagc
     6301 ggtacacctt cacctcggcc ggcaggtact cgtcgccgta ctgctcctgg atgaacgcgc
     6361 gcacctcggc aatggcgtcg ggcgagacgc gggtcgagtc ggtacgcatg taggtgatga
     6421 gccccgtggc cccctcgccc acctccacgc cctcgtagag ctgctgggcg atgaccatgg
     6481 tcttctgtcc gctgtacctg tggcggcgga aggattcctg ctggagcgtg ctcgtgatga
     6541 acggtgggac cgggttccgc ttcttctcct gcgtgcggac ctgggtgacc gcgaacgact
     6601 cgcgcgcgag cgcgtcggcg tccgcacggg cctgcttctc gttctcgagc gcgaacttct
     6661 cgcccgcctt cttctgcacg cgggcgcgga agatgtcccc cgccggcgtg acgagatccg
     6721 cgtcgatggt ccagtactcg cgcgtgacga acttccggat ctcctcctcg cgctcgcaga
     6781 tcaggcggag cgcgaccgac tgcacgcgcc cggccgagag gccatagcgg atcgtgcgcc
     6841 agaggaaggg gctcaccttg tatcccacga gacggtcgag cacgcgacgg gcctgctgcg
     6901 cctggacctt ggccatgtcg atggtgcgcg gatgctcgag cgcttcggtg acgccgcgct
     6961 tcgtgatctc gtagaacgcg agacggcgga tgtcgtcggc gtcatcgccc agcgtctcgg
     7021 caaggtgcca cgcgatcgcc tccccttcgc gatcgggatc gggcgcgaga tagacggtgg
     7081 ccgcgcggcg cgcgctcgcc ttgaggtcct tgaggatctt ccccttgccc ttgatcagca
     7141 cgtacgacgg ctcgaagccc ttctcgacgt cgacgcccag cttgctcttt ggaagatcgc
     7201 ggacgtgccc gttcgaggcc ttcacctcgt agccggggcc gagatacttc gcgatggtcc
     7261 tggccttggc cggcgactcg acgatgacga gattgcgccg gcgaccaccc gcggcgggcg
     7321 cttcgcgctt cgcggacgcc ttcttgcgcg cggctacctt cttcttggtc gcgcccttca
     7381 cgccggccgg caccttctcg acggccggcg ccggggcggc cttggccgcc gcagccacct
     7441 tcttccgtgt cgcggggcgc ggcttcgcct cggcggccgt ctcgaccgcc tcggcagcct
     7501 ctgcctgttt gaccgacttc gccttggact tcaccgcgct caatgcgccc ccgccgtcgg
     7561 agccgggtcg aacgtgaccc cgtgctcgtc atcgaaccac cggatgagaa cctcgatcgc
     7621 cagctcgcgc gcttgcgcct cgtcatggag cgacggtccc atgtcgcgag cgcgtgagag
     7681 gatctcctcg aactgctccg gcgagacctt ccccgcttcg agcagccgga acagatacgc
     7741 gtacgccgga ttcgccagca ggagcgcccg ctccgggccg ctcgggacgc gcacgttcgg
     7801 ccgctccttg gcggagccgt cgccgaagtc ctcgcgcgca tagggctcga gggcacggac
     7861 gatcatctcg aatgcctcac cgaccacgct cgcgtcgaac cggccggagt cgagcacacg
     7921 gctgagctcg aggagcgcgt cttcgtcgcc gtcgacgaaa gccttcagcc gctcgacgat
     7981 gacttgcacc atctcccgga cgctttcatc catggtgtgg gctcctttca gttagcgccg
     8041 tggcacttct tgtatttctt cccgctaccg cacgggcagg gatcgtttcg accgactttc
     8101 ggctccgtgg agaccacggg agcctgcttc gcgcccggag gcgcggcgcc cggtctgggc
     8161 ggtggtgccg cgctccggcc gccgggacgc tgagatgcgc cgctgtcctc ttgcggttcc
     8221 gccatcgtgc cgaacgcggt cacctccgca tgcacttcct gctgacgcgg cgctgactta
     8281 gccgctggac ggccaggcag aatacgcggc atctcgggag gcgcgccgga agcacccgcg
     8341 gggccgacgg caagggatgg gaggccccgc cccggaaccg gctccatcac gagcgcggcc
     8401 cggaagaggt tccgcacggt ctcctcgcgg atggaccgcg tcagctcttc gaacatctcg
     8461 aacgcttcct tcttgtactc gaggagcgga tcgcgctgcc cgtacgcacg gagcccgatg
     8521 ccctcgcgca tgtggtccat ctcgcggaga tggtccatcc agtgctcgtc gatgacctcg
     8581 agatacacca cccgctccag ctcgcgcagg atcggggcgg acatctcctg ctccttctcc
     8641 tcgtacgcct tgtggaagat ctcctggatc tcctcctcga cctcgtcggg cgacgggaga
     8701 cggtccccct ggtcggggag cttcacggga cgaaggagga gctggctcac gtcgttcagg
     8761 acccgcttca gatcctcctc gtcgtagcgc tcgtgcgatc cgagataggt gtggacccgc
     8821 tcggagacca tctcatccat catctcgagc atcgtctcct tgagatccgc ctcctcgagc
     8881 gcgcggagcc gctgcgcgta gatgaccgtg cgctgcttgt tcatcacgtc gtcgtactcg
     8941 aggaggtgct tccggatgtc gaagttgtgc gcctcgaccc gcttctgcgc gcgctcgatg
     9001 gcgcgggtga cgagcccgtg ctcgatgacc tcgccttcct gcacgccgag ccgctccatg
     9061 atcccggcga tccggtcgga gccgaagagc cgcatcaggt cgtcctcgag cgagagatag
     9121 aaccgggaga accccggatc tccctggcgt ccgcttcggc cgcggagctg gcggtcgatg
     9181 cgccggctct cgtgccgctc ggtgccgagg atgtggagcc cgcaggtcat ctcctccttg
     9241 caccgcccca cgttcctcca gacgccgttg tccagcttgc tctgctgatt cacgtagcag
     9301 ccgcggtcgc accggatgac gcccttctcg agcttgatgt cggttccgcg gccggccatg
     9361 ttcgtcgcga tcgtgacggc gccgcgccgt ccggcgttcg cgacgatctc ggcttcctgc
     9421 tggtggtatt tcgcgttcag cacgttgtgc ggaacgccct tccgcttgag aagccggctc
     9481 agggtctcgg atgcttcgac cgagatcgtg cccacgagca cgggtatgcc ctgcgcgtgc
     9541 atgctcgcga tctcatcgac gagcgcgttg tacttctcgc gccgcgtctt gaagatgacg
     9601 tcgttggaat cctcgcgacg gaccggctgg ttcgtcggga tcacgacgac gtcgcgcttg
     9661 tacgtgtgcc agaactcgct cgcctcggtc tcggccgttc cggtcatgcc tgcgagcttg
     9721 tcgtagaggc ggaagtagtt ctggatcgtg atggtcgcga gcgtctgcgt ctcgccttcg
     9781 accttcacgt tctccttcgc ctcgatcgcc tgatgcagtc cgtccgaata gcggcggccc
     9841 ggcatgaggc gccccgtgaa ctcgtcgacg atcaggatct tcccgtcctg gacgacgtat
     9901 tccacgtcct tctcgaagag cgagtacgcc ttcaggagct ggaggatgtc gtgaacccgc
     9961 tcgctccgga tggcgtgctg gcgctccagc tcgcgcttct tctggacctt gtccgccggc
    10021 gagatggcct cgtcctcgtc gatcgctccc agctcgaccg cgaggtcggg caggaggaaa
    10081 cgattcggat cgcgctgcga caggagcgtg cgcccctgct cggagagatc caccgagtgg
    10141 ctcttctcgt cgatggaata gaggagatcc tcgtccactt cgcccaggcg cttgtcgcgg
    10201 atgaagtcga gctcgacctt ctggatgagc cgcttcagtc ccggctgctc cgcgacgagc
    10261 ttgaggaagc gcttgttctt gggagcgccg cgcttgactt gtaagagctt gaccccggcc
    10321 tcgtactcct tctcggggtc ctgcagcatc gtctccgctt cggcgaggat ccggttcgtg
    10381 atctggccct ggctccgcac cacgcggtcc acgtcgggct tcatctcgtc gtactgctgg
    10441 aggctgtggt ccaccggccc cgagatgatg agcggcgtcc tcgcctcgtc gatgagaacc
    10501 gagtcgactt cgtcgacgat cgcgtagttg tgaccgcgct gcacccgatg gtcacgatgc
    10561 cgcgccatgt tgtcgcgcag gtagtcgaag ccgaactcgt tgttcgtgcc gtaggtgatg
    10621 tcggcggcgt aggccgctct cttctcctcg gtcggctggt tgctcttgat gacgccgacg
    10681 gtgagcccga ggaaccggta gacgggggcc atccaatccg cgtcgcgctg tgcaaggtac
    10741 tcgttgaccg tgacgacatg
//
LOCUS       DASTKC010000077         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_290118, whole genome shotgun sequence.
ACCESSION   DASTKC010000077 DASTKC010000000
VERSION     DASTKC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290118"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="sucC"
                     /locus_tag="VFR25_03415"
     CDS             complement(<1..999)
                     /gene="sucC"
                     /locus_tag="VFR25_03415"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET9940129.1"
                     /translation="MNLHEYQGKEIFRAAGIPVPPGEVAKTADEAVAIAGRVGYPAVV
                     KAQVLIGGRGKAGGVKVVKTPEEMAREAGRILGMDIRGHTVHKVLITPSAEIAQEFYA
                     GVVLDRKAETPLLMVSPAGGIDIEEVAHSTPEKILRLHLDSRGLPDYRAREAARFLDS
                     RWDVQKQLARVLVRLARVYRDQDCSLAEINPLVVTKKGEVWALDAKVVIDDNALDRHP
                     DLAELRDLGAEDPGEVEARERGLSYVRLDGTIGCVVNGAGLAMATMDLIQYHGGKPAN
                     FLDIGGSSNPDKVTAAMNILTRDSRVKAVLFNIFGGITRCDDVARGLLTALDRIGTK"
     gene            complement(996..2429)
                     /locus_tag="VFR25_03420"
     CDS             complement(996..2429)
                     /locus_tag="VFR25_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HET9940130.1"
                     /translation="MPRVRRVRKVVIMGAGGRDFHNFNTFFKDNRNYEVVAFTASAQI
                     PGIDDRRYPKSLAGPNYPKGIPIFSEQELPEIVRERDVDLVVFAYSDVSHEYVMHKAS
                     WVAGMGCDFMLMGPKATQIKSRVPVVAVTAVRTGSGKSQTTRKVCAVLKGMGKKVVAI
                     RHPMPYGELSRQGVQRFATYADLDRHKCTIEEREEYEPHLDNGTIVYAGVDYKEILAA
                     AEKEADVIVWDGGNNDFAFYKPDLTIVVADPHRPGHERRYHPGETNVRLADVVIINKE
                     TTTDYANIEAVRASVQQVNPDAVIIDAASPITVDRPELIRGRRALVIEDGPTLTHGEM
                     KYGAGVIAAKRLGAKEIVDPRPWLVGEIQDTFRQYPDIGPLLPAMGYSPEQIRDMERT
                     INATECDVVIVATPIDLRRLMKIRPPSVRVGYELEEIGRPKLEDVLAEALNRLTKGRK
                     PAAKRPLKSGVRRAARKAGPLPRRGSR"
     gene            complement(2653..3114)
                     /gene="rfaE2"
                     /locus_tag="VFR25_03425"
     CDS             complement(2653..3114)
                     /gene="rfaE2"
                     /locus_tag="VFR25_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET9940131.1"
                     /translation="MGEILTLEEALRRRAAARALRQTFVFTNGCFDVIHPGHVALLHQ
                     ARSLGHVLFVGLNSDRSVRALKGEGRPVQSEDARAIVLAALADVSAVVVFDEDTPLEL
                     IQALKPDVLVKGGDYAPEQVVGRDVVEKAGGRLVLVPLVPGASTSAILSRL"
     gene            complement(3107..4129)
                     /gene="rfaE1"
                     /locus_tag="VFR25_03430"
     CDS             complement(3107..4129)
                     /gene="rfaE1"
                     /locus_tag="VFR25_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET9940132.1"
                     /translation="MNPATVPASPSKLTRRSLEERIASFRGRRVLVVGDVILDRYLWG
                     RATRVSPEAPVLVVDIEREELRLGGAANVAHNVRALGADPVLIGVVGEDQAAADLDRL
                     LRSRGVDPSGLVRDPSRRTTLKTRIIAHHQQVLRADEESKDALGTAQEAALWDRVEQA
                     IGEADAALVSDYGKCVVTPSLLSKLLPALERQGVPSAVDPKEEHFFRYTGVSVITPNA
                     AEAAQAWGRRLKTEAELIEAGFGLREKLEAGSVLITRGEEGMSLFTAEGHTHFPTRAR
                     KVYDVTGAGDTVVATIATALAAKATLPEACVLANHAAGIVVAQLGTATAGAEELIASL
                     EDLPVG"
     gene            complement(4126..4416)
                     /locus_tag="VFR25_03435"
     CDS             complement(4126..4416)
                     /locus_tag="VFR25_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940133.1"
                     /translation="MTANRRDDEETEEQLVRVADVWDEATATLLCDFLNEQKIRATAV
                     SAQIPAFGMIERAGSGHWGYVEVLEHDAERAKELIRDFYAARPEAGNGEAAG"
     gene            complement(4413..5396)
                     /gene="galE"
                     /locus_tag="VFR25_03440"
     CDS             complement(4413..5396)
                     /gene="galE"
                     /locus_tag="VFR25_03440"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET9940134.1"
                     /translation="MNFLVVGGAGYIGSVVAEELARGRHSVLVLDDLSTGHKDAVPPL
                     AYLVKGPITDPKALREAFTTKEIDCVVHLAARSIVSDSMADPQGYEEANVRQGTILLD
                     AMKEHGVSRIVFSSTAAVYDASAPMPLHESSPLIPGNPYGQTKLEFEAILRERERADG
                     LRYVALRYFNVAGASIDHGEDHRRETHLIPLLLDAARGAREAVPVYGSDYPTPDGTAV
                     RDYIHVVDIAEAHVRAIRHLMDGGVSLTLNLGAERGLSVREVIRSVERVTGRTVPTVE
                     APRRAGDPAALIASSEEAERVLGWKPQWGDIDTIIETAWAWRQRYPEGYAE"
     gene            complement(5393..6028)
                     /gene="rpe"
                     /locus_tag="VFR25_03445"
     CDS             complement(5393..6028)
                     /gene="rpe"
                     /locus_tag="VFR25_03445"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9940135.1"
                     /translation="MSADFARLGDEIQRVERAGADFLHIDIMDGQFVRNITFGPILVE
                     AMRRLSTLPFDVHLMIVEPLRFLEPFAKAGADHLIVHEETVPDLREAARAIRARGLKA
                     GGVVKPGTTIERLIAALPDLDIALVMTVEPGKGGQAFLEDSPGRIARVREAIDQGGYD
                     CLLQVDGGIGEETIVEAERAGADTFVAGHSIFRTPDPELALRRLRAAAGRA"
BASE COUNT          877 a         2096 c         2049 g         1007 t
ORIGIN      
        1 cttggtgccg atccggtcga gcgcggtgag gagcccgcgc gcgacgtcgt cgcagcgggt
       61 gatgccgccg aagatgttga agaggaccgc cttgacgcgc gagtcgcgcg tcaggatgtt
      121 catcgcggcg gtgaccttgt cgggattgct cgagccgccg atgtcgagga agttggccgg
      181 ctttccgccg tggtactgga tcagatccat cgtcgccatc gcgaggccgg cgccattcac
      241 gacacagccg atcgtgccgt ccaggcggac gtaggagaga ccgcgctccc gcgcctccac
      301 ctcgcccgga tcctcggcgc cgagatcgcg cagctcggcg agatccggat ggcgatcgag
      361 cgcgttgtcg tcgatcacca ccttggcgtc cagcgcccag acctcgccct tcttggtcac
      421 gacgagcgga ttgatctcgg cgagcgagca gtcctgatcg cgatagacgc gcgcgaggcg
      481 gacgagaacg cgagcgagct gcttctgcac gtcccaccgc gagtcgagga atcgcgcggc
      541 ctctcgggca cggtagtcgg gaagtccgcg cgaatcgagg tggaggcgga ggatcttctc
      601 gggggtggag tgcgcgacct cctcgatgtc gatgcctccg gccggactca ccatgaggag
      661 cggcgtctcg gccttccggt cgaggacgac gcccgcgtag aactcctgcg cgatctcggc
      721 gctcggcgtg atcaggacct tgtgcacggt gtgtccgcga atgtccatgc cgagaatgcg
      781 ccccgcctcg cgcgccatct cctcgggcgt cttgaccacc ttcacgccgc ccgccttgcc
      841 acgccctccg atcaggacct gcgccttgac cacggccgga tagccgaccc ggcctgcgat
      901 cgcgaccgcc tcgtccgccg tcttggccac ttctcccggc ggcaccggaa tcccggccgc
      961 gcggaagatc tccttcccct ggtattcgtg aagattcatc ggctccctcg tctcggaagt
     1021 ggcccggcct tccgtgccgc gcggcgaacg ccgctcttga gcggccgctt ggctgccggc
     1081 ttgcgtccct tggtgaggcg gttgagcgcc tcggccagca cgtcttccag cttcggccgc
     1141 ccgatctcct cgagctcgta cccgacgcgc accgacggcg ggcggatctt catgaggcgc
     1201 cggaggtcga tcggcgtcgc gacgatgacg acgtcgcact ccgtggcgtt gatcgtccgc
     1261 tccatgtccc ggatctgctc gggcgagtag cccatcgccg gcaggagcgg cccgatgtcc
     1321 ggatactggc ggaacgtgtc ctggatctcg ccgaccagcc agggccgcgg atccacgatc
     1381 tccttcgcgc cgagccgctt cgccgcgatc acgccggcgc cgtacttcat ctcgccgtgc
     1441 gtgagcgtgg ggccgtcctc gatcacgagc gcgcggcgtc cgcggatcag ctccggacga
     1501 tcgaccgtga tcggcgaggc ggcgtcgatg atcaccgcgt cgggattcac ctgctgcacc
     1561 gacgcgcgaa cggcctcgat gttcgcgtag tcggtcgtcg tctccttgtt gatgatcacc
     1621 acgtccgcga ggcggacgtt ggtctcgccc ggatggtagc ggcgctcgtg cccggggcga
     1681 tgcggatcgg ccacgacgat cgtgagatcc ggcttgtaga aggcgaagtc gttgttgccg
     1741 ccgtcccaca cgatcacgtc cgcctccttc tcggcggcgg ccaggatttc tttgtagtcg
     1801 acgccggcat agacgatcgt gccgttgtcg aggtgcggct cgtactcctc gcgctcctcg
     1861 atcgtgcact tgtgccggtc gaggtcggcg taggtggcga agcgctgcac gccctgcctc
     1921 gacagctcgc cgtagggcat cgggtggcgg atcgcgacca ccttcttccc catccccttg
     1981 agcaccgcgc aaaccttccg cgtcgtctgg ctcttccccg atcccgtgcg cacggcggtc
     2041 accgcgacca cggggacccg gctcttgatc tgggtcgcct tcggccccat gagcatgaag
     2101 tcgcatccca tccctgcgac ccaggaagcc ttgtgcatca cgtactcgtg ggagacgtcg
     2161 ctgtaggcga agaccaccag atcgacgtcg cgctcgcgca cgatctcggg cagctcctgc
     2221 tcgctaaaga tggggattcc cttcgggtag ttgggcccgg ccagcgactt cggatatctc
     2281 cgatcgtcga tgcccgggat ctgggcggag gcggtgaacg ccaccacctc gtagttccgg
     2341 ttgtccttga agaaggtgtt gaagttgtga aaatcgcgcc cgccggcgcc catgatgacg
     2401 acctttcgca cgcgtctcac tcgaggcata cctccttcta gggtgtggcg atccggcggc
     2461 accggccgag ggctcgtcga gcttcgagtc gctcggcgag cgcggcggcg cgccatcggt
     2521 tcgtcgttcg gtgttgactt cgaagaggtc tagaaccgga cgacgttacc ggcacgcgcg
     2581 cgtgagtgtc aagcgtgcga cgggaagcct tggcttcgca aggtgttgca ggaagcccgg
     2641 atgcgggcag ggctacaggc gggacaggat ggccgaggtg gacgcgccgg gaacgagggg
     2701 cacgaggacc aggcgtcccc ccgccttctc gacgacatcg cgccccacaa cctgctccgg
     2761 ggcgtagtcc ccgcccttca cgagcacgtc cggcttcagg gcctggatca gctcgagcgg
     2821 ggtgtcctcg tcgaacacca cgacggcgct cacgtccgcg agcgcggcga ggacgatcgc
     2881 gcgcgcgtcc tcactctgga ccggacgccc ctcgcctttc agcgcgcgca cggaccgatc
     2941 cgagttgagt ccgacgaaga gcacgtgccc caggcttcgc gcctgatgca ggagagccac
     3001 gtgcccggga tggatcacgt cgaagcagcc gttcgtgaac acgaaggtct gccggagcgc
     3061 tcgcgcggcg gcgcgccggc ggagcgcctc ctcgagcgtg aggatttcac ccaaccggaa
     3121 ggtcctcgag cgacgcgatc agctcctcgg ctccggcggt cgcggttccg agctgcgcga
     3181 cgacgatccc cgcggcgtga ttcgcgagaa cgcatgcctc gggaagcgtg gccttggcgg
     3241 cgagcgcggt cgcgatcgtg gcgacgaccg tgtcccccgc gccggtcacg tcgtacacct
     3301 tgcgcgcgcg cgtcgggaag tgcgtgtgcc cctcggccgt gaagagcgac atcccttcct
     3361 cgccgcgcgt gatcaggacc gatcccgcct cgagcttctc gcggagcccg aatcccgctt
     3421 cgatcagctc ggcctcggtc ttgagccggc gcccccacgc ctgtgccgcc tccgcggcgt
     3481 tcggcgtgat gacgctgacc cccgtgtagc ggaagaagtg ctcctccttg ggatcgaccg
     3541 cgctcggtac gccctggcgc tcgagggccg gaaggagctt cgataggagc gagggagtga
     3601 cgacacactt gccgtagtcg ctcacgagcg ccgcgtccgc ctcgccgatc gcctgctcca
     3661 cgcgatccca gagcgccgct tcctgcgccg taccgagcgc gtccttgctc tcctcgtcgg
     3721 cgcgaagcac ctgctggtgg tgcgcgatga tgcgcgtctt gagcgtggtg cgccgggagg
     3781 gatcgcggac gagccccgac ggatcgacgc cgcgcgagcg gaggaggcga tccagatcgg
     3841 cggcggcctg atcctcgccg acgactccga tcagaacggg atcggcgccg agcgcgcgaa
     3901 cgttgtgcgc gacgttggcc gcgccgccca ggcgaagctc ttcacgctcg atgtcgacga
     3961 cgaggaccgg cgcctccggc gagacgcgcg tggcgcgtcc ccagagatag cggtcgagga
     4021 tgacgtcgcc gacgacgagg acgcgccgtc cccggaacga tgcgatccgt tcctcgagcg
     4081 atctcctcgt gagcttggaa ggcgatgcgg gcacggtggc tgggttcaac cggcggcctc
     4141 tccgtttcca gcttccggtc gcgcggcgta gaagtcgcgg atgagctcct tcgcgcgctc
     4201 ggcgtcgtgc tcgagcacct cgacgtagcc ccagtgcccg ctgcccgccc gctcgatcat
     4261 gccgaaggcg ggtatctgcg ccgacacggc ggtggcgcgg atcttctgct cgttcaggaa
     4321 gtcgcagagc agggtcgcgg tcgcttcgtc ccagacgtcg gcgacccgga cgagctgctc
     4381 ttccgtctcc tcatcgtctc tacggttcgc ggtcattcgg cgtatccctc cggataccgc
     4441 tggcgccagg cccacgcggt ctcgatgatg gtgtcgatgt ccccccactg cggcttccag
     4501 cctaacacgc gctccgcctc ttccgacgag gcgatgagcg cggcgggatc gcccgcgcgg
     4561 cggggcgcct cgacggtggg aacggtccgg cccgtgaccc gctccaccga gcggatgacc
     4621 tcgcggacgg agagcccgcg ctccgcgccc agattgagcg tgaggctgac gccgccgtcc
     4681 atcagatgcc ggatcgcgcg cacgtgcgcc tcggcgatgt cgaccacgtg gatgtagtcg
     4741 cgcaccgcgg tgccgtcggg cgtcgggtag tcgctcccgt agaccggaac cgcctcgcgg
     4801 gcgccgcgcg ccgcatccag taggagcggg atcaggtgcg tctcccggcg atggtcctcg
     4861 ccgtggtcga tgctcgcccc cgccacgttg aaatagcgga gcgccacgta gcggagcccg
     4921 tccgcgcgct cccgctcgcg caggatcgct tcgaactcga gcttcgtctg tccgtacgga
     4981 ttccccggga tcagaggcga gctctcgtgg agcggcatgg gcgccgacgc gtcgtagacc
     5041 gcggcggtcg aggagaagac gatccgactc accccgtgct ccttcatcgc gtcgaggaga
     5101 atcgtcccct gccgcacgtt ggcttcctcg tatccctgcg ggtcggccat ggagtcggag
     5161 acgatcgagc gggccgcgag gtggacgacg cagtcgatct ccttcgtcgt gaacgcctcg
     5221 cgcagcgcct tgggatcggt gatggggccc ttcacgagat aggcgagcgg tggcaccgcg
     5281 tccttgtgcc cggtcgagag gtcgtcgagc acgaggaccg aatggcgccc ccgcgcgagc
     5341 tcttcggcga cgacgctccc gatgtaaccc gcgcctccga cgacgaggaa attcacgcgc
     5401 gcccggccgc ggcccggagg cggcggagcg ccagctcggg atcgggggtc cggaagatcg
     5461 agtggccggc gacgaacgtg tccgcgccgg cgcgctcggc ttcgacgatc gtttcctcgc
     5521 cgatgccgcc gtctacctgt aagagacagt cgtacccgcc ctgatcgatc gcctcgcgca
     5581 cgcgcgcgat gcgaccgggc gaatcctcca ggaaggcctg tccccccttc ccgggctcca
     5641 ccgtcatgac gagcgcgatg tcgagatcgg gcaatgccgc gatcaaccgc tcgatcgtcg
     5701 tgcccggctt caccacgcct cccgccttga gcccgcgcgc gcggatggcg cgcgccgcct
     5761 cgcgaaggtc gggcacggtc tcctcgtgca cgatcaagtg atccgccccc gccttcgcga
     5821 acggctcgag gaagcggagc ggctccacga tcatgaggtg gacgtcgaac ggaagcgtgg
     5881 aaagacggcg catggcctcg acgagaatgg gaccgaacgt gatgttgcgg acgaactgcc
     5941 cgtccatgat gtcgatgtgg aggaaatcgg cccccgcgcg ctccacgcgc tggatctcgt
     6001 cgccgagacg cgcgaagtcg gcggacaga
//
LOCUS       DASTKC010000078        13367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1710352, whole genome shotgun sequence.
ACCESSION   DASTKC010000078 DASTKC010000000
VERSION     DASTKC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710352"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..851)
                     /locus_tag="VFR25_03450"
     CDS             complement(351..851)
                     /locus_tag="VFR25_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940136.1"
                     /translation="MNTARLASSPRVAALLAVLVILAGCNKSGTKTEASTDMAGSASS
                     TAAAMGQSLMDQLGGMEGVVKLADAFGANLAANPALSSLLNADAITGAKQGLVNEIAK
                     ASNTPAPYAGASLAGSLSGKGLTHDSVKAVTDALESAADSMHLTPATKSSLMGLMAPI
                     SKALGG"
     gene            complement(907..1491)
                     /locus_tag="VFR25_03455"
     CDS             complement(907..1491)
                     /locus_tag="VFR25_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HET9940137.1"
                     /translation="MLLANELKRKSIHLSSIAIPLAYFFTPDSWQKGWERGLLALVIL
                     SLAIEVFRLNHPRTRHIFRHFFGELLRNHEEASLLGSTYLLIACLLTIHLFPKDIAVL
                     ALCFLILGDTMAAIVGKSIGKIRMFGKTLEGSLACFAVCYGLTLIMPGPNLPFPVGLV
                     GAAVATVFELLPIPLDDNFRIPLSAGFAMELLLR"
     gene            complement(1493..3853)
                     /locus_tag="VFR25_03460"
     CDS             complement(1493..3853)
                     /locus_tag="VFR25_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9940138.1"
                     /translation="MARRGRGLLLGAWTAALAAAAAFALLPGAMICGAETVWKEGAKK
                     MSAERSAWDQARRAYRTARWSGSLPEIAAAFSQAARAESTYLGALEAWIRSGAVGFAG
                     RPDPTFEGTLRDGALDLMEDGRVESAKLLLGGPLRFDASLAPLHARAVGLGAPDSGLA
                     ILGWPPDRRGGAARFGADITGRFSGPSADASYLVAAELSDSVDLPRARRAALWRLQSH
                     PRPAVRSYARMALARSLLASGEPRLAGAVLSSASAMNPDEEALLVDIRADLMESAGDT
                     LGAVNLLIDAVRSGKGTLAARYPLAMRAAGEARGARVDSLDENRWFDLVKQLGTMGES
                     HAALGLLDARRAPPRMANAEFNRAELRASLLARLKRHDEAVLGYQRLLTRKDLSSEAR
                     ARLALGLARSLRAQGSFAAMDAAFVRAVVVDPKGPNASQAAWERAREWEDRRSPTEAA
                     AIFAWARPYVGDPAIAPPLAAHAGIAMLRAGKPDSAVIAITDSIATVPRYWRARARFA
                     AGDTAGAYAALGAIAQGEPWTYEGVRAREELQRVGAPWPGAAAPADSDSSAEAMVRAT
                     PERELDPPLPARVLGGAGAVPLLMDLLRNCAKNGDPGQARGCTDELEERGVFRVGRAG
                     LLPQARLEYPPAYPTAVLHAAERESLAAALLWAIMRQESIYQRNARSKAGAIGLLQLM
                     PFTASRLNGSPVTEGSLTDPDLNVRLGAAYLRGLLQEFKDPRAAMAAYNAGEDAVRRW
                     LRDRPVVDDVWVEMIPYRETRDYVKQVYSIWRRYEALYGSGSAGRP"
     gene            complement(3856..6645)
                     /locus_tag="VFR25_03465"
     CDS             complement(3856..6645)
                     /locus_tag="VFR25_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET9940139.1"
                     /translation="MSHDRAVRAALSLTLLIFVAAPAWAAAPSPKVYRNLTEVQRSAA
                     IKAYDQARVNLRAMGVDKIYPQFALDMAKFPRQGVAHRNVLVVLCKFPSEGGSPSSGP
                     AAVSTPRYFYNHLFSDDPSDGMTSLREYYRTNSNGRLVISGYVTSDWLEMPHSYEYYV
                     NDYAGLNFATYPRSAQKLAEDAMDAAYNDFDGNLKFFDNDGPDGVPSSGDDDGYIDAV
                     MVIVPGAGGETQCSVPQGCQRLWAHESGIALYSDCPQANSGPDCLPGLPLGDKRGFLY
                     NLGSEYNEYPGDNAVGTWIHEFSHTLGLPDLYDLSGGNGLGFYSLMAVGNYLPYSGDP
                     NTTGPGVQGANPSNLDAWSRQFLGFDNPTPAAAGHYVLPPVTRGGGSIKIWTNGEPGT
                     EYFLVENRTKEGSDAFLPGEGLLVYHIDDTKPDNIDGAANYRARIVAADNLGQLESGA
                     NYGDPADFFPGTTGKTNITESTSPNTRDFNLFDTGIRITNIAYAADTVSFDLQIATKP
                     DFRTVRYTIDDGGGDGLPDPNEAEQLTLTMKNVGIPSGPLTLTISTGDPAVTLNTATV
                     AAPSALGGATVTTSSPFAYTIGSIVNLPHLVHFNVNWSDGTYNGVFGFDVVVGAQPGL
                     SEDFESGNEPGLFWETESLPGSSSNQWHAEQQRAKSGTWSAKVGSTATSGAPEDLSYA
                     PMQDAALVSPTFELPAGSQLAFDSWIDAETYGGTESVDGGRVELSMNGGEWVPLPVDG
                     GYGTIIKFDSASSLRGQDVFSGSPQSWRHVTADLSGYSGEARVRFRFSSNEDNPPFGN
                     FGEQVRAYEGWYVDNVVVQTRTAPTSTARAVSLRGGPSPYHIGAPSAGSLQFRFTAPD
                     GHPHPELSPVVRIFDVRGRLIRELPAATGLVASEFHATWNAKDRNGDTCRAGIYFARV
                     DIQGKEQTFRVVLLK"
     gene            complement(6677..8215)
                     /gene="guaA"
                     /locus_tag="VFR25_03470"
     CDS             complement(6677..8215)
                     /gene="guaA"
                     /locus_tag="VFR25_03470"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9940140.1"
                     /translation="MSHERLLILDFGSQFTQLIARRARELGVFAEIRAPNLDAMTLAS
                     LNPKGIILSGGPSSVDEPGAPQLDPAVLAQGVPILGVCYGMQLLARDLGGRIHPSKHR
                     EYGQAVLQVERAGTLLAGLPAESRIWASHGDIVEELPPGFTLLARSTGGLAAAAEDLD
                     HQRYVVQFHPEVAHTEQGMRIYRNFLFDVCGFSGDWTMGQVLDEQTEKIRRQVGKRRV
                     LCALSGGVDSSVVAALLHRAIPGQVRAVFVDHGLLRRNEAAQVQELMQHVIGDDLVTV
                     DARRRFLDALQGIEDPEAKRKTIGRVFIEVFEDEAKRLGGAELLAQGTLYPDVIESTS
                     IRGPSAKIKSHHNVGGLPEHMKMELVEPLRELFKDEVRSLGSLLGLPPSVLGRHPFPG
                     PGLAVRILGPVTEEAVETLGLADDIYITELRRADLYDKVWQAFAVLLPVRTVGVMGDG
                     RTYDQVVALRAVTSSDGMTADWARLPHEFLARVSSRIVNEVRGVNRVVYDVSSKPPAT
                     IEWE"
     gene            complement(8212..8721)
                     /locus_tag="VFR25_03475"
     CDS             complement(8212..8721)
                     /locus_tag="VFR25_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9940141.1"
                     /translation="MGRFKVYQAARRDSLDPWIEFDPGTDRKQRVFQFSGHDTLLLRD
                     GDMGYALENSGVDLYVRAISGSDSLPHLPDGDVLYDQRPRPIWAPPPEYPEFARTARI
                     TGRVVLDVLVDEKGHVRDVFVIQSVMGLDQPAIEAAKVWTFTPARKNGVPVAAWFELP
                     FDFQLPEGP"
     gene            complement(8964..10559)
                     /gene="purH"
                     /locus_tag="VFR25_03480"
     CDS             complement(8964..10559)
                     /gene="purH"
                     /locus_tag="VFR25_03480"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET9940142.1"
                     /translation="MIGRALLSASDKTGLVEFARGLSRHGVKILSTGGTALALRGAGI
                     TVRDVSEVTGYPELFDGRVKTLHPSIHGGILYRRGLGRDEEDRHKHGIVSIDLVVVNL
                     YPFERTVASQATTDDEAIEQIDIGGPALIRAAAKNHSHVVVVVDPDQYGVVLEAMDRQ
                     GGVPPSIARKFARRAFERTATYDAAISRYLAGTERDTPGETRAPAAGVPGALEGVAAL
                     PETIDLQLRIEQRLRYGENPGQEGAWYLPEGGGRLKLGQMRGKTLSFNNLLDVEAALG
                     LLREFSEPAAVVIKHRNPSGAAVADTAAEAIGRARQGDELSAFGGILGLNRPLDDAAL
                     EAVGSFFYEIVLAPSVEANEAKLAALRKNLIILELPWMREASASGLSARTLLGGLLAE
                     TVPGPPRLEGWKAVSKTPPSPAQLEDLHFAWRVVRHAISNGIVIAHGGRTLGIGAGQS
                     SRVDAVRLALLKAERAGHDVRGAVLCSDAFFPFPDSVEIAAEAGIAAIAHPGGSVRDG
                     ESVAAADEAGIALLTTGERCFLH"
     gene            complement(10568..11470)
                     /locus_tag="VFR25_03485"
     CDS             complement(10568..11470)
                     /locus_tag="VFR25_03485"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET9940143.1"
                     /translation="MKTADRALRDVVIPGRAPDYKGKVRDLYDLGSQLLLVATDRVSA
                     YDVILNEAIPGKGRVLTQISRFWFERLESIVPSHYLTTDVARFPEPFASNRSVLDGRS
                     MLVKKAKRYDVECVVRGYLAGSGWKEYQASGMVCGVKLPPGLKLSSKLPEPIFTPATK
                     EQEGHDENIDFDRMASIVGGDVAEKLRATSIAIYKAAAAHAESIGLILADTKFEFGEK
                     DGAIVWIDEALSPDSSRYWPIDRYTEGVAQDSFDKQVIRDYLDSIKWDRNPPPPRLPD
                     EVIEKTARRYEDAFAMITGARAAR"
     gene            complement(11483..12052)
                     /gene="purN"
                     /locus_tag="VFR25_03490"
                     /pseudo
     CDS             complement(11483..12052)
                     /gene="purN"
                     /locus_tag="VFR25_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
     gene            complement(12084..>13367)
                     /locus_tag="VFR25_03495"
     CDS             complement(12084..>13367)
                     /locus_tag="VFR25_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9940144.1"
                     /translation="LFVQSGGATIGTAGVQSTMFGAIPMVGLGLNLIVIPLCTLFMAE
                     CVLFLGAAATGVPLLRDAAAGALEASGLLMLELNTHGSRALDPWLVHGVAPLGALALG
                     ALFLALAGAASESCRGGRTSRSSAARWSAAALFVGALVPFVPSPRRAPSVPTIVMLDI
                     GQGDATWVSLPGSGSLLVDAGPRDERRDSGESVIEPALRAEGAGRIANALLSHAHLDH
                     FGGFEWLARREWIGTLWENGRDRRGAWRRGLARALGRHGGRALGVARDMTLALGGGAS
                     LTVERGPVDGPHENDASLAGILRAGGATALFAGDLETDGEEALLPRLGPVDVVKAPHH
                     GSKTSSGEDWVAKLHPSVVLVSSGEHNRFHHPNPTVLGRYERWGARVLRTDQEGAIRL
                     TLVPGGAWISTRAHPAPERVTWNRDAPVTPSREEP"
BASE COUNT         2122 a         4551 c         4589 g         2105 t
ORIGIN      
        1 tgccgaggat tcgtgtcagg aggttcagca tggcttcctt attggatgcg gtcagggccc
       61 cgtgcgccct ctacccgcgg tttccgggcc ggttccggct cccgtccccc ctagtatggc
      121 aaaaccccag gagcctctgg ccgccggggg ttcgatacgc gaatgctgcc ttctaagctc
      181 ttgctgtttc gctgattagc tcgctcgtcg tgcctcggcc atgctctgtt ctttggcggg
      241 cagtctaggg gccgagccgg ggggtgtcaa gctggaatga ccaatctaag tagaaggctc
      301 caggccccaa tcatccctga gtaaccgctt gtggtgggcg gctggcgccc ctagcccccc
      361 aacgccttcg agatcggggc catgagtccc atgagcgacg acttggttgc cggggtgagg
      421 tgcatggagt ccgcggcgct ctccaacgcg tccgtgaccg ccttcaccga atcatgggtc
      481 aaacccttcc ccgagagcga tccggccagg ctggcgcccg cgtagggcgc cggagtgttg
      541 ctcgccttcg cgatctcgtt cacgagcccc tgcttcgcgc ccgtgatggc atccgcgttg
      601 agcagggagg acaaggcggg gttcgccgca aggttcgctc cgaaggcatc ggctagcttg
      661 acgactcctt ccatcccccc gagctgatcc atgagcgact gacccatggc ggcggcggtg
      721 ctcgacgcgg agccggccat gtccgtagag gcttcggtct tcgtgccgga tttgttgcaa
      781 ccggccagaa tcacgagcac ggcgagcagg gcggctacgc gaggggacga ggcaaggcgg
      841 gcggtattca tgggatcctc cgtggagtcc agcattgaga cgggcggtcc ggggttggcg
      901 gcgtgactac cgcaagagta gctccatcgc gaacccggcg gatagcggaa tgcggaagtt
      961 gtcgtccagc gggatgggca ggagctcgaa cacggtcgcc accgcggcac ccaccaaccc
     1021 caccgggaac ggcaggttcg ggccgggcat gatcagcgtg agcccgtagc agaccgcgaa
     1081 gcacgcgagg gagccttcga gcgtcttccc gaacatccgg atcttgccga tcgacttccc
     1141 cacgatcgcg gccatcgtgt ccccgaggat caggaagcag agggcgagca cggcgatgtc
     1201 tttcgggaac agatggatcg tgagcaggca cgcgatcagc aggtaggtcg atccgagaag
     1261 gctcgcttcc tcgtggtttc gcagaagctc cccgaagaaa tgccggaaga tgtgccgcgt
     1321 gcgtggatgg ttcagccgga acacttcgat cgcgagcgac aggatcacga gcgcgaggag
     1381 tccgcgctcc cagcccttct gccacgagtc cggcgtgaag aaatacgcga gtgggatcgc
     1441 gatcgaggag aggtggatcg atttccgttt cagctcgttg gcaaggagca ccctagggcc
     1501 tgcccgccga gcccgagccg tagagcgcct cgtagcggcg ccagatcgaa tagacctgct
     1561 tcacgtagtc gcgggtctcc cggtacggaa tcatctccac ccacacgtcg tccaccaccg
     1621 gtcggtcgcg gagccaccgg cgcaccgcgt cctcgccggc attgtaggct gccatggccg
     1681 cccgtgggtc cttgaattct tggaggagcc cgcgaaggta ggcggccccc agccgcacgt
     1741 tgaggtcggg gtccgtgaga gatccctcgg tcacgggcga gccgttcaac cgggaagctg
     1801 tgaacggcat gagctgaagg agtccgatcg caccggcctt cgagcgtgcg ttccgctggt
     1861 agatgctctc ctggcgcatg atcgcccaga ggagcgccgc ggcgaggctc tcccgctcgg
     1921 cggcatggag gaccgcggtc gggtacgcgg gcggatactc caggcgtgcc tgaggcagaa
     1981 gtcccgcgcg gccgacgcgg aacacgcctc gttcctcgag ctcgtccgtg cagcctcgcg
     2041 cctgcccggg atcgccgttc ttggcgcaat tgcgcaacag gtccatcagc aacggcacgg
     2101 cgccggcgcc gccgagtacg cgcgcgggca gaggcggatc gagctcgcgc tcgggagtgg
     2161 cgcgcaccat cgcctccgcg gaagagtcgg agtccgcggg agctgccgcc ccgggccacg
     2221 gcgcaccgac gcgctggagc tcctcacggg cgcgcacgcc ttcataggtc cagggctctc
     2281 cttgcgcgat ggctccaagc gccgcgtacg ctcccgccgt gtcgcccgcg gcgaaccgcg
     2341 cgcgagcgcg ccagtatcgc ggcacggtgg cgatcgagtc ggtgatggcg atcaccgcag
     2401 agtccggctt ccccgcccgc agcatcgcga tcccagcgtg cgcggccagc ggcggtgcga
     2461 tggcgggatc gccgacgtac ggacgcgccc acgcgaagat cgcggctgcc tccgtgggac
     2521 tccggcgatc ctcccactcg cgggcgcgct cccaggccgc ctgcgaggcg ttcggcccct
     2581 tgggatccac cacgaccgca cgcacgaatg cggcgtccat cgccgcgaac gagccctgcg
     2641 cgcggagcga ccgcgcgaga ccgagcgcca atcgggcgcg cgcctcggag gacaaatcct
     2701 ttcgcgtcag gaggcgttgg tatccgagaa ccgcctcgtc gtgacgcttc agccgggcga
     2761 ggagcgatgc acgaagctcc gcgcgattga actccgcgtt cgccatgcgg ggcggcgcgc
     2821 gccgcgcatc gaggagccca agcgcggcat ggctctcacc catcgtcccg agctgcttca
     2881 ccagatcgaa ccagcggttc tcgtcgagtg aatcgacgcg cgcccccctg gcctcgccgg
     2941 cggccctcat cgcgagcggg tagcgggccg cgagcgttcc cttgccggag cgtacggcgt
     3001 cgatcaggag gttcacggcg ccgagggtgt cgccggccga ctccatgaga tccgcgcgga
     3061 tgtcgacgag gagcgcttcc tcgtccgggt tcatcgccga ggcagacgag agcacggcgc
     3121 cggccaaccg aggctccccg ctcgccagca acgatcgtgc gagcgccatg cgagcgtagc
     3181 tccgcacggc gggacgcgga tggctctgca aacgccagag cgcggcgcgg cgtgcgcgcg
     3241 ggagatcgac agagtcggac agctcggcgg cgacgaggta ggaagcgtcg gcggagggac
     3301 cggaaaaacg ccccgtgatg tcggcgccga atcgcgccgc accgccccga cgatccggcg
     3361 gccatccgag gatcgcgagc cccgagtcgg gtgcgccgag tccaaccgcg cgggcgtgga
     3421 gcggagccag cgaagcgtcg aatcggagtg gaccgccgag gaggagcttc gccgactcga
     3481 cccggccgtc ttccatcaga tcgagcgcgc cgtcgcggag cgtaccttcg aatgtgggat
     3541 caggccgccc ggcaaacccc acggcgcccg aacgaatcca cgcctcgagc gccccgaggt
     3601 aggtcgactc ggcgcgcgcg gcctgcgaga aggcggccgc gatctcgggg aggctgccgc
     3661 tccagcgcgc ggtgcggtac gcgcgccgcg cctgatccca ggcggagcgc tcggcgctca
     3721 tcttcttggc gccttccttc cacaccgtct cggcgccgca gatcatggcg cccggcagga
     3781 gcgcgaaggc agcggccgcg gcgagcgcgg ccgtccatgc cccgagaaga agcccccgcc
     3841 cccgccgcgc cacgcctact tcagcagaac cacgcggaag gtttgttcct tgccctggat
     3901 gtcgactctg gcgaagtaga tgcccgcgcg gcaggtgtcc ccgtttcgat ccttcgcgtt
     3961 ccacgtcgcg tggaactcgc tcgcgacgag gccggttgcg gcgggaagct cgcggatgag
     4021 gcggccgcgc acgtcgaaga tgcgcacgac gggactgagc tccggatgcg ggtggccgtc
     4081 gggcgcggtg aagcggaact ggagcgagcc ggccgaagga gcgccgatgt gatacgggct
     4141 cgggccgccg cgcagggaca cggcccgcgc ggtcgacgtg ggtgccgtgc gcgtctggac
     4201 gacgacgttg tccacgtacc agccttcgta cgcgcggacc tgttcgccga agtttccaaa
     4261 gggcggattg tcctcgttcg acgagaagcg gaagcgcacc cgcgcctctc cggagtatcc
     4321 ggagaggtcg gctgtgacat gacgccagct ctgcggcgat ccggagaaga cgtcctgacc
     4381 gcggagcgag gaggcggagt cgaatttgat gatcgtgccg tagccgccgt ccaccgggag
     4441 tgggacccac tcgccgccgt tcatcgagag ctcgacccgg cctccgtcca ccgactccgt
     4501 accgccgtag gtttcggcat cgatccagga gtcgaatgcg agctggctcc cggccggaag
     4561 ctcgaacgtg ggcgagacga gggcggcgtc ctgcatcggc gcgtagctca agtcctcggg
     4621 cgcgccggac gtggcggtcg accccacctt cgcgctccag gtcccgctct tcgcacgctg
     4681 ctgctccgcg tgccactggt tgctcgagct gccgggaagc gactcggtct cccagaagag
     4741 gccgggctcg ttgcccgact cgaagtcctc ggacaggcct ggctgcgctc ccacgacgac
     4801 gtcgaacccg aacacgccgt tgtaggtgcc gtcgctccaa ttcacgttga agtggaccag
     4861 gtgcggcagg ttcacgatcg atccgatcgt gtaggcgaac ggtgaagatg tcgtgaccgt
     4921 ggctccacct agagccgaag gcgctgccac cgtggccgtg ttcagcgtca ccgcagggtc
     4981 cccggtgctg atggtgaggg tcaaggggcc ggacggaatc ccgacgttct tcatcgtgag
     5041 cgtgagctgc tcggcttcgt tcggatccgg aagtccgtcg ccaccgccat cgtcgatcgt
     5101 gtaccgcacg gtgcggaagt ccggcttggt cgcgatctgc aggtcgaacg acaccgtgtc
     5161 cgcggcgtag gcgatgttcg tgatccggat gccggtgtcg aagagattga agtcgcgcgt
     5221 attgggcgac gtcgattcgg tgatgttcgt ctttcctgtc gtccccggga agaagtcggc
     5281 cggatcgccg tagttggcgc cgctctcgag ctgcccgaga ttgtcggccg cgacgatgcg
     5341 cgcgcggtag ttcgccgccc cgtcgatgtt gtccggcttg gtgtcgtcga tgtggtagac
     5401 gaggagaccc tcacccggta ggaacgcgtc cgatccttcc ttcgtccggt tctcgacgag
     5461 gaagtactcg gtcccgggct cgccgttcgt ccagatcttg atcgagccgc cgccgcgcgt
     5521 gaccggcggg agcacgtagt gtcccgccgc ggccggcgtg ggattgtcga atccgaggaa
     5581 ttggcgggac catgcgtcga ggttcgaggg attcgccccc tggacacccg gtcccgtcgt
     5641 gttcgggtcg cccgagtacg gtaggtagtt cccaaccgcc atgagcgaat agaagcccag
     5701 cccgttcccg ccgctcaggt cgtagagatc cggcaggccg agcgtgtggc tgaactcgtg
     5761 gatccaggtt cccaccgcat tgtcgcccgg gtactcgttg tactcgctcc cgaggttgta
     5821 gaggaacccg cgcttgtcgc cgagcggcag gcccggcaga cagtcggggc ccgagttcgc
     5881 ctgggggcaa tcggagtaga gcgcgatccc cgactcgtgc gcccagagcc tctggcagcc
     5941 ctgcggcacg gagcactgcg tctcgccgcc ggcgccaggc acgatgacca tgaccgcgtc
     6001 gatatagccg tcgtcgtcgc cgctcgacgg caccccgtcc ggtccgtcgt tgtcgaagaa
     6061 cttgagattg ccgtcgaagt cgttgtacgc ggcgtccatc gcgtcctcgg cgagcttctg
     6121 ggccgagcgg ggataggttg cgaaattgag ccccgcgtag tcgttcacgt agtactcgta
     6181 gctgtgcggc atttcgagcc agtcggacgt gacgtagccc gagatgacga gccggccgtt
     6241 cgagttggtt cgatagtact cgcgaaggct cgtcatcccg tcgctcgggt cgtcgctgaa
     6301 gagatggttg tagaagtacc gcggcgtgct cacggccgcc gggcccgagg agggcgagcc
     6361 gccctccgac gggaacttgc agagcacgac gagcacattt cgatgcgcca cgccctgtcg
     6421 tgggaacttc gccatgtcga gcgcgaactg cgggtagatc ttgtccacgc ccatcgcccg
     6481 tagattgacg cgcgcctggt cgtaggcttt gatcgcggcg ctccgctgga cctcggtgag
     6541 gttccgatag accttcggag atggagccgc cgcccaggca ggcgcggcca cgaagatgag
     6601 aagcgtcagg gacagcgcgg cacgcacggc tcgatcgtgg ctcaacggat tgtctccgga
     6661 tggatgatgg gaagcctcac tcccactcga tcgtggcggg aggcttgctg ctgacgtcgt
     6721 acacgacgcg attgacccct cggacttcgt tgacgatgcg cgacgagacg cgcgcgagga
     6781 actcgtgcgg cagccgggcc cagtcggccg tcatcccatc gctcgaggtg accgcgcgga
     6841 gcgcgaccac ctggtcgtac gtccggccgt cccccatcac cccgaccgtc cgcaccggga
     6901 gaagaaccgc gaaggcctgc cagaccttgt cgtacaggtc cgcgcggcgc agctccgtga
     6961 tgtagatatc gtccgcaagg ccgagtgtct ccaccgcttc ctccgtgacc ggccccaaaa
     7021 tccgcacggc cagaccgggc cccgggaagg ggtgccggcc tagaacggag ggggggagac
     7081 ccagaagaga tccgaggctt cggacctcgt ccttgaataa ctcccgaagg ggctccacca
     7141 gttccatctt catgtgttcg gggaggccgc ccacgttgtg atgtgacttg atcttggccg
     7201 agggcccccg gatggacgtg gactcgatga cgtccgggta gagggtgccc tgggccagga
     7261 gctccgctcc ccccaatcgc ttggcctcgt cctcgaagac ctcgatgaag acccgtccga
     7321 tcgtcttccg cttggcctcg gggtcctcga tgccctggag ggcgtccagg aagcgccgcc
     7381 gggcatccac ggtcacgagg tcgtccccga tcacgtgctg catcagctcc tgcacctggg
     7441 ccgcctcgtt cctccggagc agtccgtggt ccacgaagac cgcccggacc tgccccggga
     7501 tggcccggtg gaggagggcc gccacgaccg aggagtccac gccccccgag agggcgcaga
     7561 ggacccggcg cttccccacc tggcgacgga tcttttcggt ctgctcgtcc aggacctgcc
     7621 ccatcgtcca gtcgccggaa aagccgcaga cgtcgaagag gaagttccgg tagatgcgca
     7681 tcccctgctc cgtgtgcgcg acctcgggat ggaactggac gacgtagcgc tggtggtcga
     7741 ggtcttcggc cgcggcggcg agccctcccg tcgagcgggc caggagcgtg aagccggggg
     7801 gcagctcttc gacgatgtcg ccgtggctcg cccagatccg gctctccgcc ggcagccccg
     7861 cgaggagcgt tcccgcccgc tccacctgga gcacggcctg cccgtactcg cggtgcttgc
     7921 tcggatggat gcgcccgccg aggtcgcgcg ccaggagctg catgccgtag cagacgccga
     7981 gaatcgggac gccctgcgcg agcaccgcgg ggtcgagctg cggcgctccg ggctcgtcca
     8041 cgctcgaggg accgcccgag aggatgatcc ctttcgggtt gagcgatgcc agcgtcatgg
     8101 cgtcgaggtt cggtgcgcgg atctcggcga agacgccgag ctcacgcgcc cggcgcgcga
     8161 tgagctgggt gaactgcgaa ccgaagtcga ggatcagcag tcgttcgtgg ctcacggccc
     8221 ctccggtagt tgaaagtcga agggcagctc gaaccaagcc gcgactggaa ctccgttctt
     8281 cctggccggt gtgaaggtcc acaccttggc cgcctcgatg gccggctggt cgagacccat
     8341 cacgctctga atgacgaaga catctcggac gtgccccttc tcgtcgacga ggacgtcgag
     8401 cacgaccctt cccgtgattc gcgcggttcg ggcgaattcg ggatactcgg gcggcggagc
     8461 ccagatcggc ctcggtcgct ggtcgtagag aacgtcacca tcgggcaggt ggggaaggct
     8521 gtcgctgccg gagatggcac gaacgtacag gtcgaccccg ctgttctcga gcgcataacc
     8581 catgtccccg tctcgaagga gcaacgtatc gtggccggag aactggaaca cgcgctgctt
     8641 cctgtcggtc ccgggatcga actcgatcca tggatcgaga gagtctcgac gagcggcttg
     8701 atacaccttg aatcggccca tccgcgtcct ggatcctccg ttcctgatct cggtcaatcg
     8761 gtacgtcgag tcggcattca gactgagttg accgtcacgt cccggaccac gcgtgaacgt
     8821 atcattggac tgccacctcc acgttccgaa gaggctgtcc gcataaacct cttcgatggg
     8881 cgcggggtga tcgatggtgg cacagcctgc gcctgccaga accccgaggc ccacgatggg
     8941 cgcatactcg cgcagcatcc gtctcaatgt agaaaacatc tctcgcctgt ggtcaaaagc
     9001 gcgatccccg cctcgtccgc ggcggcgacg gattctccgt cccgcaccga tcctcccgga
     9061 tgcgcgatcg ccgcgatccc cgcctcggcc gcgatctcga ccgagtcggg gaacgggaag
     9121 aacgcgtcgg agcagagcac cgcgccgcgc acatcgtgcc cggcccgctc cgctttcaac
     9181 aaagcgagcc gcaccgcgtc cacgcgcgag gactgccccg ccccgatccc gagcgttcgc
     9241 ccaccgtgcg cgatcacgat gccgttcgag atcgcgtggc gcaccacgcg ccaggcgaag
     9301 tgcagatcct cgagctgcgc ggggctcggc ggcgtcttgg agaccgcctt ccatccctcg
     9361 agccgcggcg gccccggcac ggtctccgcc aggaggccgc ccagaagcgt gcgcgcggac
     9421 agacccgacg ccgacgcttc gcgcatccag ggcagctcta ggatgattag attcttgcga
     9481 agggccgcga gcttcgcctc gtttgcttcc accgaggggg cgagcacgat ctcgtagaag
     9541 aacgagccga ccgcttcgag cgcggcgtcg tcgagcgggc ggttcaagcc caggatcccg
     9601 ccgaacgccg agagctcgtc cccctgccgc gcccgcccga tcgcctccgc cgcggtgtcc
     9661 gccacggcgg caccggaggg attccggtgc ttgatgacga cggccgccgg ctcgctgaac
     9721 tcgcgcagca ggccgagcgc cgcctccacg tcgaggaggt tgttgaagga gagcgtcttc
     9781 ccgcgcatct ggccgagctt gagccggccc ccgccctcgg ggagatacca ggccccctcc
     9841 tgtccgggat tctcgccgta gcgaagccgc tgctcgatcc ggagctggag gtcgatcgtc
     9901 tcgggaagcg ccgccacgcc ctcgagcgcc ccgggcacac cggccgccgg cgcgcgcgtc
     9961 tcgcccggcg tgtcgcgctc ggtgcccgcg aggtagcgcg agatcgccgc gtcataggtc
    10021 gccgtgcgct cgaaggcgcg ccgagcgaac ttccgggcga tcgacggtgg aacgcctccc
    10081 tggcgatcca tcgcctcgag gacgacgccg tattgatcgg gatccaccac gacgaccacg
    10141 tggctgtggt tcttcgccgc cgcgcggatg agcgcggggc cgccgatgtc gatctgctcg
    10201 atcgcctcgt cgtcggtcgt agcctgcgac gcgacggtcc tctcgaaggg atagaggttc
    10261 acgaccacga gatcgatcga gacgatgccg tgcttgtggc gatcctcctc gtcccggccg
    10321 aggccgcggc ggtagaggat cccgccgtgg atcgaggggt ggagcgtctt cacgcgcccg
    10381 tcgaacagct ccgggtaccc ggtcacctcg gagacatcgc gcacggtgat gccggcgccg
    10441 cgaagcgcga gcgcggtgcc gcccgtcgag aggatcttca cgccgtggcg cgagagtccg
    10501 cgcgcgaatt cgaccagacc ggtcttgtcg gacgcggaga gcagcgcccg cccgatcacg
    10561 atggcgctca ccgcgcggcg cgcgcccccg tgatcatcgc gaaggcatcc tcgtagcgcc
    10621 gcgcggtctt ctcgatcacc tcgtccggca ggcgaggcgg cggcggattg cgatcccact
    10681 tgatggaatc gagatagtcg cggatcacct gcttgtcgaa gctgtcctgc gccacgcctt
    10741 cggtgtagcg atcgatgggc cagtaacgcg acgaatcggg cgagagcgcc tcgtcgatcc
    10801 agacgatcgc cccgtccttc tcgccgaact cgaacttggt gtcggcgagg atgagtccga
    10861 tcgactccgc gtgcgcggcg gccgccttgt agatcgcgat gctcgtcgcg cggagcttct
    10921 cggcgacgtc accgccgacg atcgacgcca tgcggtcgaa gtcgatgttc tcgtcgtgcc
    10981 cttcctgctc cttcgtcgcg ggggtgaaga tcggctcggg aagcttcgac gagagcttga
    11041 gccccggcgg gagcttcacg ccgcagacca tgccgctcgc ctggtattcc ttccaccccg
    11101 atcccgcgag atagccgcgc acgacgcact cgacgtcgta gcgcttggcc ttcttcacga
    11161 gcatggaccg tccgtcgagc acggaacgat tggaggcaaa gggctcggga aagcgcgcca
    11221 cgtcggtcgt gaggtagtgg ctcgggacga tcgactcgag acgctcgaac cagaagcggg
    11281 agatctgggt gagcacgcgc cccttcccgg ggatcgcctc gttcaggatc acgtcgtacg
    11341 ccgacacgcg gtcggtcgcg accaggagga gctgcgagcc gagatcgtag agatcgcgga
    11401 ccttcccctt gtagtcgggc gcgcggcccg ggatcaccac gtcgcgcagg gcgcgatcgg
    11461 ccgtcttcat cgattcctcc tgagtgaacg ttcgtatcgc gtcgacgtag atcgtgtgtt
    11521 ccagcggcag cagacgctct gcaagcgagt ccgcggtctc acccttttga atcggaagct
    11581 ccgcctggag gatcacgggg cccgagtcca tgcccgcatc cacgcggtgc acggtcgctc
    11641 cggtcaccgc agcgcccgcc tcgatcgcct gccgctgcgc ctccagccca ggaaatcgcg
    11701 ggaggagcga cgggtgcacg ttgaggatcg gggcgcccgc gcggtcgatc gtctccgcgc
    11761 gcagaaggcg catgaagccg cagagcgcga tcagcccggg acgatcccgg cggatgatgc
    11821 cttggagctc gtcttcggct tcgggagcga gccgcgatcc ggggcgccac ggcgtgagga
    11881 cggacgtggg gatcgatcgc tctcgggcgt actcgagcgc gggagcgtcc tgccggtcgg
    11941 agacgacccg cacgaccgct gctcgcagcc gtccgtcgcg cgtggcctcg atcagattcg
    12001 cgacgtggct cccccgcccg gaggcgagga gcagcagccg aaggggctcc atcgacaatc
    12061 gataccatac tcgggcgtcc ggctcaaggt tcttcgcgtg aaggggttac cggcgcgtca
    12121 cggttccacg tcacgcgctc cggagccggg tgcgcgcggg tcgagatcca ggcgccgccc
    12181 ggcaccagcg tgagccggat cgccccctcc tgatcggtgc ggaggacccg agcgccccat
    12241 cgctcgtacc ggcccagaac cgtcgggttg ggatgatgga agcggttgtg ctcacccgag
    12301 gacacgagga cgaccgaggg atggagcttg gccacccagt cctcgccgct cgacgtcttg
    12361 ctcccgtgat gcggcgcctt gaccacgtcg acgggtccga gccgcgggag gagcgcttcc
    12421 tccccgtccg tctcgaggtc gcccgcgaag agcgcggtcg caccgccggc gcggagaatg
    12481 cccgcgagcg aggcgtcgtt ctcatgcggt ccgtcgacag ggcctcgctc gaccgtgagc
    12541 gaggctccgc cgcccagcgc tagcgtcatg tcgcgcgcga ctccgagagc gcgtcctccg
    12601 tgacgtccga gcgcacgcgc gagccctcgc ctccacgcgc cgcggcgatc gcgtccgttc
    12661 tcccataggg tcccgatcca ttcgcgccgg gcgagccact cgaatccgcc gaagtgatcg
    12721 agatgcgcat gcgagaggag cgcgttcgcg atccgccccg ccccctccgc gcggagggcg
    12781 ggctcgatca ccgactcgcc ggagtcgcgc cgctcgtcgc gcgggcccgc gtcgacgaga
    12841 agggatccac tccccggaag cgagacccac gtggcatctc cctgcccgat gtcgagcatc
    12901 acgatcgtgg gcacggaggg tgcgcgccgt ggggacggaa cgaacgggac gagcgcgccc
    12961 acgaagagcg ccgccgcgga ccagcgcgcc gcggaggagc gcgacgtgcg gccgccgcga
    13021 cacgattccg acgcggcccc cgcgagcgca aggaagagcg cgccgagagc gagcgcgccg
    13081 agcggcgcga cgccgtgcac gagccacggg tcgagcgccc gcgatccgtg cgtgttgagc
    13141 tccagcatca ggagtcccga cgcctcgagc gcacccgccg cggcgtcgcg caggagcggc
    13201 acgcccgtcg cggcggcgcc gagaaagagc acgcactccg ccatgaagag cgtgcagagc
    13261 gggatcacga tgaggttgag cccgaggccc accatcggga tcgcgccaaa catcgtcgac
    13321 tggacacccg cggttccgat cgtggccccg ccgctctgca cgaagag
//
LOCUS       DASTKC010000079         7473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_765056, whole genome shotgun sequence.
ACCESSION   DASTKC010000079 DASTKC010000000
VERSION     DASTKC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765056"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFR25_03500"
     CDS             <1..366
                     /locus_tag="VFR25_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9940145.1"
                     /translation="AWEERDPDQAAALFADECVYRSSPFRTPHQGRDGVRAYWQRATD
                     DQRSLDLQFGTPILQGLRVAVEWWAILKTAGETGVWEAGTLPGCLYLIFDEDGRCVEL
                     REYWHWKDGVMSAYDGWGA"
     gene            complement(400..1470)
                     /locus_tag="VFR25_03505"
     CDS             complement(400..1470)
                     /locus_tag="VFR25_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940146.1"
                     /translation="MASLTIAGLSLVYPNGTRAVDSIEIALADGEFLAILGPSGCGKS
                     SLLRLIAGLEPPTSGTIAMNGRDVTALEPKDRDVAMVFQGLALYPHMTVEENMAFGLL
                     ARKTPRAEIAERVREAGTMLGLTRYLAKKPRELSGGERQRVALGRALVRRPQIYLFDE
                     PLSSLDAQLRQELREELARLHRVTRTTSIYVTHDQKEALSLGDKVAVMSAGTLRQVAS
                     PETVYREPHDLFVARFVGEPAINLIEGQIAVDAGAASFRAPGLRLALNGSRARSGPAT
                     LGLRAEAIALVPGVRDAEGSAVVERIEHLGGDTLVHLKGAWGGLVARLSDAASAPAPG
                     ATVEVRPDPKRALFFGPDGARI"
     gene            complement(1472..2566)
                     /locus_tag="VFR25_03510"
     CDS             complement(1472..2566)
                     /locus_tag="VFR25_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET9940147.1"
                     /translation="MTDPGSDKPRVVKGDQKFDLTLERFRERYLARFYDPEFEMVPEE
                     LERIVSVAWEAYHQYRKNPRTRKAGPEFANPDANLPVEWLDTRQHILEAERRQKDAVS
                     PSRILVVNGSARTDQSCPGEISKTYRLAKIVQGTILAEQGFEVDFLDLSALTAEYGLV
                     IYPCKACVSTAQPLCHWPCSCYPNHAMGQVNDWMSELYPRWTAAHGVMILCPVNWYQA
                     PSVLKLMIDRLVCADGGNPDPTTTRGKNPALAKEIELRGWSYPKHLAGRAFSVVVHGD
                     AAGAETLRRMLVDWMKDLEMVPAGPNPGLDRYIGYYEPYATSHDDLDKDTAIQEEVRH
                     AALTLCETVRQIRTGRYHRPDEGLEEPRKK"
     gene            2706..3182
                     /locus_tag="VFR25_03515"
     CDS             2706..3182
                     /locus_tag="VFR25_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940148.1"
                     /translation="MMRATLRISTLAVVSAVFLATNAWAGYPAGVRGGFSSDPDQFVF
                     GVQMEFPPLAENLYVVPSAEVGIGDDAFSLAVNGDLQYRFHTSSGIRPYVGGGLNLYY
                     FDPDADNADSDTNIGVSALGGLFFGSANGPPLFVDVKLGLTDEVPDWKFMFGVMFK"
     gene            complement(3227..4051)
                     /locus_tag="VFR25_03520"
     CDS             complement(3227..4051)
                     /locus_tag="VFR25_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9940149.1"
                     /translation="MTHRSRLSPARTLLVLAMVGLAVLAASQEKTLSIERAVTGQDPG
                     KTPEISTQELRLILVSGSATVFDARPPAEYGMGHIPGARNVAPKPGAPTSEYVSDAAE
                     IGRVVNGNKKAPIVLYCNGPFCGKSKRLADELATAGFTNVRRYQLGIPVWRALGGPCE
                     MSFQAFFDILQKDKTAVVIDAREADAFRALSLPGSRNIPRSLVQEGKDVGEIKKAKDD
                     GRLPMDDHNTRIIVIASDGASARYVAGALTYEAFQNVNYLEGSFAQTKAAGDSLSK"
     gene            complement(4086..5192)
                     /locus_tag="VFR25_03525"
     CDS             complement(4086..5192)
                     /locus_tag="VFR25_03525"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET9940150.1"
                     /translation="MPDELAQLRARIAALDTEIVRLAAERFALVREVGETKRAEGVPV
                     RSFTAEADVIARYRAAAREKGLPEGFAERLALQLIAGAVQLQEEALPGSRPAASRRIT
                     IVGGAGKMGRWLAEYFAGQGHEVSIHDPAGDVPGFPSEAGLEDVARNAEIVVLATPLG
                     SGRAVLQKVLALEPKGIVADIFSLKSHVMDLLRNAASQGQPVTSLHPLYGPDVRTLSG
                     RTLAVCDCGHAGATEEATALFQDTALTITLIPVERHDEIMQYVLGLSHLVSILFFTTL
                     TESGVPFATLEPMASTTFHKQMRAAAAVASESPQLYYEIQKLNRHSAELFEKVRASLE
                     LIEQAAHAGDSASFTKIMNRGHEYFPLVLPQELD"
     gene            complement(5203..6306)
                     /locus_tag="VFR25_03530"
     CDS             complement(5203..6306)
                     /locus_tag="VFR25_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9940151.1"
                     /translation="MNQFVPEVTDADVERIIERDYPRDKHTEIRDLVRGVEVREKPRV
                     MLACLKNGKGDFEKLKGELTNASGWYRESILEAEYPNYRKKMFRIDRLPPEEQERIVE
                     ADKAQYLKWLVTEPLPDYVLQNRGHWDELAKSYAASAARHWAEPSPSWGIWGIPESEV
                     GMIPADIAGKDVIELGCGTAYVAAWLARAGARVTGIDNSEAQLATARRMQIEHGLDFP
                     LVHGNAETVPCPDASFHFAVSEYGACLWADPYKWVPEVARILRPGGRLHFLTNGYVHT
                     LCAPELETESATDRLRRPAFGLHRLEWPDDTGVEFHLTHGSWIRLFRENGFQIHELLE
                     LRPPEGATTRFPYVTAEWARQWPSEEVWKVRKR"
     gene            complement(6308..6853)
                     /locus_tag="VFR25_03535"
     CDS             complement(6308..6853)
                     /locus_tag="VFR25_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940152.1"
                     /translation="MPFDLQPTLEGEILELRPLRPEDFDALFAVASDPLIWELHPERD
                     RYKEERFRAYFQSGLDCGGALLAIDRKTGAVIGSSRYYGYDPEASEIEIGWTFLARSH
                     WGGEYNGEMKRLMLGHAFRFVENVVFLIGADNLRSRKAVEKIGAVYIGTRIDSKREER
                     VVYRITASSWNPLTDPRSWQV"
     gene            complement(6856..>7473)
                     /locus_tag="VFR25_03540"
     CDS             complement(6856..>7473)
                     /locus_tag="VFR25_03540"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET9940153.1"
                     /translation="LRYIGYVVGACAASVIFAGMAGTDPWRPEFMPILNTTAIVIALA
                     TTLMILGAEFLARHRDRLGPSERVMPEVAATLANFILLAWWAREAGHLASVVATPGER
                     LGSLEPTARTLAAVFTSAAWTLQAVTLFGLGWIRDSAFLRWSGLVLFGLTVLKFLLVD
                     LDRVDTFWRFVSALGIGVALLVVSFLYQRRARRSAEEPPRQAEAG"
BASE COUNT         1181 a         2521 c         2512 g         1259 t
ORIGIN      
        1 gcctgggagg agcgcgaccc ggaccaggcg gctgcgctct ttgcggacga atgcgtgtac
       61 cgatcgagcc cgtttcggac cccgcaccag gggcgcgacg gcgtgcgcgc gtactggcag
      121 cgggccacgg acgatcaacg ctcgctggat ctgcaatttg ggacgccgat cctccaggga
      181 ctgcgcgtcg cggtggagtg gtgggcgatc ctcaagaccg ccggcgagac cggagtttgg
      241 gaggccggga cgctccccgg ctgcctgtac ctcatcttcg acgaggacgg ccgctgtgtg
      301 gagctgcgcg agtactggca ctggaaggat ggtgtcatgt ccgcgtacga cgggtgggga
      361 gcataggcga ctgttacgag cggcagcgac cgccgcgatc tagatccgcg cgccgtccgg
      421 tccaaagaag agcgcgcgct tgggatcggg acggacctcc acggtcgctc cgggcgcggg
      481 agccgatgct gcgtccgaga gacgcgcgac gagcccgccc cacgctccct tgagatgcac
      541 cagcgtgtcg cctcccaagt gctcgatccg ctccaccacc gccgagccct cggcatcgcg
      601 aacccccggc acgagcgcga tcgcctccgc ccggagtccc agcgtcgcgg gcccgcttcg
      661 ggctcgcgat ccgttgagcg cgaggcgaag ccccggcgcg cgaaacgacg cggcgcccgc
      721 atccacagcg atctgccctt cgatcaagtt gatcgcaggc tcgccgacga accgcgccac
      781 gaagagatcg tgcggctcgc gatacaccgt ttcgggcgag gccacctgcc gcagcgtgcc
      841 cgcgctcatc accgcgacct tgtctccgag cgagagcgcc tccttctgat cgtgcgtgac
      901 gtagatcgac gtcgtgcggg tgacccgatg gagccgcgcc agctcctcgc gcagctcctg
      961 gcggagctgg gcgtcgaggc tcgagagggg ctcgtcgaag agatagatct gcggccggcg
     1021 cacgagcgcg cgccccagcg ccacgcgctg gcgttcgccg ccggagagct cgcgcggctt
     1081 cttcgcgagg tagcgcgtga ggccgagcat cgtcccggcc tcgcgcacgc gctccgcgat
     1141 ctcggcgcgc ggcgtctttc gcgcgaggag cccgaacgcc atgttctcct cgaccgtcat
     1201 gtggggatag agcgcgagcc cctgaaacac catggccacg tcccgatcct tgggctcgag
     1261 cgcggtcacg tcgcggccgt tcatcgcgat cgtccccgac gtgggcggct ccagcccggc
     1321 gatgagcctg aggagcgagg acttgccaca gcccgacggg ccgaggatcg cgaggaactc
     1381 tccgtccgcg agcgcgatct cgatggaatc gaccgcgcgc gtgccgttgg gatagacgag
     1441 ggacaagccc gcgatcgtca gggatgccac gtcacttctt tctcggctcc tcgagcccct
     1501 cgtcgggacg gtggtagcgg ccggttcgga tctggcggac cgtctcgcac agcgtgagcg
     1561 ccgcgtggcg cacttcttcc tgtatggctg tgtccttgtc gagatcgtcg tggctggtcg
     1621 cgtagggctc gtagtagccg atgtagcggt cgagccccgg attcggtccg gccgggacca
     1681 tctcgaggtc cttcatccag tcgacgagca tgcggcgcag cgtttccgcc cccgccgcat
     1741 ccccgtgaac gaccacggag aacgcgcggc cggcgagatg cttgggatag gaccatcctc
     1801 gaagctcgat ctccttcgcg agcgcgggat tcttgccgcg cgtggtcgtg ggatcgggat
     1861 tgccgccgtc cgcgcagacg agccggtcga tcatcagctt gagcacgctc ggcgcctggt
     1921 accagttcac ggggcaaagg atcatcacgc cgtgcgccgc ggtccaccga gggtagagct
     1981 cgctcatcca gtcgttcacc tggcccatcg cgtggttggg gtagcacgag cagggccagt
     2041 ggcaaagcgg ctgggccgtg gacacgcacg ccttgcacgg atagatcacg aggccgtatt
     2101 cggccgtgag ggcgctcaag tcgaggaagt ccacctcgaa gccctgctcc gcgagaatcg
     2161 tcccctggac gatcttcgcg aggcggtagg tcttcgagat ctcgccgggg cacgactggt
     2221 cggtgcgcgc ggaaccgttg acgacgagga tccgcgaggg cgatacggca tccttctgcc
     2281 ggcgctctgc ctcgaggata tgttgccgcg tgtcgagcca ctccacgggg agatttgcgt
     2341 ccgggttcgc gaactccggg ccggccttcc tcgtacgcgg gttcttgcgg tactggtgat
     2401 aggcctccca cgccacgctc acgatccgct ccagctcctc gggaaccatc tcgaactccg
     2461 gatcgtagaa gcgggcgagg tagcgctcgc gaaaccgctc gagggtgaga tcgaacttct
     2521 gatctccctt cacgacgcgg ggcttgtcgg agcctggatc ggtcatggca gacctacgct
     2581 accctccggc gacgcgtggt tgcaaccccg cgctccatgc tagcctcgat agtggagcac
     2641 ggaccagaaa cgacccccgt tccagcgacc agccggggcg gggatcctta tcaacgtagg
     2701 gggccatgat gcgagccacg cttcgaatct cgactctcgc cgtagtgtcc gccgtgtttc
     2761 tcgccacaaa cgcgtgggcc ggctaccccg ccggcgtgcg cggtggattc agctccgatc
     2821 ccgaccagtt cgtgttcggc gtccagatgg aatttccgcc cctggccgag aacctgtacg
     2881 tggtgccaag cgccgaggtg gggattggcg acgacgcgtt cagcctcgcc gtgaacgggg
     2941 atctccagta ccgcttccac acctcgagcg ggatccgccc ctacgtggga ggcggactca
     3001 atctctacta cttcgatccg gacgccgaca acgcggacag cgacaccaac atcggtgtga
     3061 gcgccctggg cggactcttc ttcggcagcg cgaacggccc gcccctcttc gtcgacgtga
     3121 agctcgggct cacggacgag gttccggact ggaagttcat gttcggagtg atgttcaagt
     3181 aggaacgtcg ggccggcgcg ccctcgaatg gcgcgccggc cacgcttcat ttcgagagcg
     3241 aatctcccgc cgccttcgtc tgcgcgaagc tcccctccag atagttcacg ttctgaaagg
     3301 cctcgtacgt gagcgccccc gcaacgtagc gcgccgacgc cccgtcggac gcgatgacga
     3361 tgatccgggt gttgtggtcg tccatgggga gccgtccatc gtccttggcc ttcttgatct
     3421 cgcccacgtc cttgccctct tgcacgagac ttcgcgggat gttgcggctc cccgggagcg
     3481 agagcgcccg gaacgcgtcc gcctcgcgcg cgtcgatcac gaccgcggtc ttgtccttct
     3541 ggagaatgtc aaagaacgcc tggaacgaca tctcgcacgg accgccgaga gcgcgccaca
     3601 ctggaatgcc gagctgatag cggcgcacgt tcgtgaaccc cgcggtggcg agctcgtccg
     3661 cgagccgctt gctctttcca cagaaggggc cgttgcaata gagcacgatc ggcgccttct
     3721 tgttcccgtt caccacgcgg ccgatctccg ccgcgtcgga gacgtactcg gacgtagggg
     3781 cgcccggctt gggcgccaca ttccgcgcgc ccgggatgtg ccccattccg tactcggcgg
     3841 gcggccgtgc gtcgaacacg gtggcggagc ccgacacgag aatcagccgg agctcctggg
     3901 tggagatctc cggcgtcttg cccgggtcct ggcccgtgac ggcgcgttcg atcgacagcg
     3961 tcttctcctg ggacgcggcc aggaccgcga gtccgaccat cgcgagcacg aggagcgtgc
     4021 gcgcagggct gagcctggat cggtgcgtca tgacgcctcc gtgtttcggg atcctcgcga
     4081 tcccatcagt cgagctcctg aggcaggacg agcgggaagt actcgtgccc tcgattcatg
     4141 atcttggtga acgaggccga gtcgcccgca tgagcggcct gctcgatcaa ttcgaggctc
     4201 gcccgcacct tctcgaacaa ctccgccgag tgccggttca gcttctggat ctcgtagtag
     4261 agctgcgggc tctcgctcgc caccgccgcc gcggcgcgca tctgcttgtg gaacgtggtg
     4321 ctcgccatgg gttcgagcgt cgcgaaggga actccgctct cggtgagcgt ggtgaagaaa
     4381 aggatcgaga ccaggtggga gagcccgagc acgtactgca tgatctcgtc gtggcgctcc
     4441 acggggatga gggtgatcgt gagcgccgtg tcctggaaga gcgcggtcgc ctcctcggtc
     4501 gcgccggcgt gcccgcagtc gcacaccgcg agggtccggc ccgacagcgt gcggacgtcc
     4561 ggaccgtaga gcggatggag gctcgtcacg ggctgcccct gcgacgcggc gttgcggagc
     4621 aggtccatga cgtggctctt caaactgaag atgtccgcga cgatgccttt gggctcgagc
     4681 gccaggacct tctggagcac cgcgcgcccg gagccgagcg gcgtcgcgag cacgacgatc
     4741 tcggcgttgc gtgcaacgtc ctccaggccg gcctcggagg gaaagccagg gacgtcgccg
     4801 gccgggtcgt ggatcgagac ctcgtgtccc tgtccggcga agtattccgc gagccagcgc
     4861 cccatcttgc ccgcgccgcc gacgatcgtg atccggcgtg aggccgcggg acgcgacccg
     4921 ggaagcgcct cttcctggag ctgaaccgct ccggcgatga gctggagcgc cagacgttcc
     4981 gcgaatccct cgggaagccc cttctcgcgg gccgctgcgc ggtagcgcgc gatcacgtcc
     5041 gcctcggcgg tgaagctccg gaccggcacg ccctccgccc gtttcgtctc gcccacctcg
     5101 cgcacgagcg cgaaccgctc cgcggcgagc cgcacgatct cggtgtcgag cgcggcgatg
     5161 cgggctcgga gctgggcgag ttcatcgggc atggcgtcca ccttagcgct tccgcacctt
     5221 ccagacctcc tccgacggcc actgccgcgc ccactccgcg gtgacgtagg ggaagcgcgt
     5281 cgtcgcgcct tccggcgggc ggagctccag cagctcatgg atctggaacc cgttctcgcg
     5341 aaagagccgg atccagctcc cgtgcgtgag gtggaactcg acgcccgtgt cgtccggcca
     5401 ttcgagacga tggagcccga acgcggggcg ccggaggcgg tcggtggcgg actcggtctc
     5461 gagctctggt gcgcagagcg tgtgcacgta accgttcgtg aggaagtgaa gccgtccgcc
     5521 cgggcggaga atccgcgcca cttccggcac ccacttgtag ggatcggccc agaggcatgc
     5581 gccgtactcg gagaccgcga agtggaagct cgcgtcgggg caggggacgg tctcggcgtt
     5641 gccgtggacg agcgggaagt cgaggccgtg ctcgatctgc atgcgtcgcg cggtcgcgag
     5701 ctgggcttcg gagttgtcga tgccggtgac cctggccccg gcgcgtgcga gccatgccgc
     5761 gacgtacgcg gttccacacc cgagctcgat cacgtctttg cccgcgatgt cggcggggat
     5821 catgccgacc tcggattccg ggatgcccca gatgccccag cttgggcttg gctccgccca
     5881 gtgccgcgcg gcggacgcgg cgtagctctt ggccagctcg tcccagtgcc cgcggttctg
     5941 gaggacgtag tcgggaaggg gctcggtcac gagccatttc agatactgag ccttgtcggc
     6001 ctcgacgatg cgctcctgct cctcgggcgg caggcggtcg atccggaaca tcttcttcct
     6061 gtagttcggg tactcggctt ccaagatgct ctcgcgatac catccgctcg cgttcgtaag
     6121 ctcgcccttc agcttctcga agtctccctt gccgttcttg aggcatgcga gcatgacccg
     6181 cggcttctcc cgcacctcga cgccgcggac caggtcgcga atctcggtgt gcttgtcacg
     6241 agggtagtcg cgctcgatga ttcgctcgac gtcggcatcc gtcacttccg gcacgaattg
     6301 attcacgtca cacctgccac gatcggggat cggtcaaggg attccagctc gaagccgtga
     6361 ttctatagac gacccgctcc tcccgcttcg agtcgatcct cgtcccgatg tagaccgcgc
     6421 cgatcttctc caccgctttc cgggagcgca ggttgtcggc tccgatcagg aagaccacgt
     6481 tctcgacgaa ccgaaacgcg tggcctagca tgagccgctt catctcgccg ttgtactcgc
     6541 cgccccagtg ggagcgcgcg aggaaggtcc atccgatctc gatctcgctc gcttccgggt
     6601 catagccgta gtagcgggac gatccgatga cggcccccgt cttccggtcg atcgcgagga
     6661 gcgcgccgcc gcagtcgagc cccgactgaa agtacgcgcg gaagcgttcc tccttgtagc
     6721 gatcacgctc cgggtgaagc tcccagatga gtgggtccga ggccacggcg aagagcgcgt
     6781 cgaaatcctc ggggcgtaac gggcgcagct cgaggatctc gccctcgaga gtcggctgga
     6841 ggtcgaacgg catggctatc ccgcctccgc ctgccgaggc ggctcttccg cggaccgcct
     6901 cgcccgccgc tgatacagga acgacaccac gagaagcgcc acgccgatcc cgagcgcgct
     6961 cacgaagcgc cagaacgtgt ccacgcgatc caggtccacg agcaagaact tcagcacggt
     7021 gagcccaaac aaaaccagtc cgctccatcg cagaaaggcc gagtcccgga tccatccgag
     7081 cccgaagagg gtcacggcct gaagcgtcca cgccgcgctc gtgaagacgg cggcgagtgt
     7141 tcgggcggtg ggctcgaggg agccgaggcg ctcgccaggc gtcgcgacga cgctcgccag
     7201 atgtcccgcc tcgcgcgccc accacgcgag aaggatgaag ttcgccagtg ttgcggcgac
     7261 ctcgggcatg actcgctcgg aaggcccaag ccggtcgcga tggcgcgcga ggaattcggc
     7321 acccaggatc atgagcgtcg tcgccaaggc gatcacgatc gccgtcgtgt tgaggatggg
     7381 catgaattct gggcgccagg gatcggtgcc ggccatgccg gcgaagatca cggaggcggc
     7441 acaggcgccg acaacgtagc caatgtatcg aag
//
LOCUS       DASTKC010000080         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_61748, whole genome shotgun sequence.
ACCESSION   DASTKC010000080 DASTKC010000000
VERSION     DASTKC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61748"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VFR25_03545"
     CDS             <1..1527
                     /locus_tag="VFR25_03545"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET9940154.1"
                     /translation="PVEARGWSRERLRVVFLHEVAHVARHDGVGLLLARVATSVFWFH
                     PLVWKLAHVARQECERCCDDMVLAAGERATDYAAHLLAIVRSMPRQHVFADVAPALAQ
                     GSNLERRLASILRSGQRRDSVSRARFAGTIVFAGLVLAATTVVHVVAAPESENEIWAS
                     VKKANREARQHAREVEAAMEAAAGSGSEMKVSDPNQTGPCDEPCAPVAPSIMSAPSAM
                     SVSVAPEVSMVPEVSVVPEVSVVVPDVSVRVSPVFSAVGSSHRRKSYDSNSSGIELMR
                     DGQYSRAITAFQEEIRQTGSTNAMYNLACAYALKGDKKLAFDALQKSIENGFDNTHHM
                     TEDEDLRSLQGDPHFYELVRLARDLQLFNSGHFGGMNGEKDWSGLLPRLERVTREHPG
                     VGRAWANLGFARLEAGDAKGGVAAYQKALDLGYEKPTTLYNLACCAARSGDIDGAFKY
                     LDRADQAGMEIGEHVGSDSDLDALRGDPRYGAMLKRWDEKMAKEHRAKGGAEVKPRTD
                     "
     gene            1730..>2307
                     /locus_tag="VFR25_03550"
     CDS             1730..>2307
                     /locus_tag="VFR25_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9940155.1"
                     /translation="MEDRWGDGPASGADDLAQLVYLSNLIGQEPRLVQPGGGNTSIKV
                     GDALLVKGSGTDLRTIGREGFTRLSLSALAALRTVETMLDADMMRLMARAMLTEGPTP
                     SVETPLHSLLAARVIAHTHDIATMSLTNVSDATAEQLVTELFAGEIVYVPYVRPGFPL
                     ARTISGIVDRLPLGTIGLALAHHGIVVWGEQPR"
BASE COUNT          413 a          737 c          761 g          396 t
ORIGIN      
        1 ccggtagagg cgcgtgggtg gagtcgcgag aggctccgcg tcgtgttcct ccacgaggtg
       61 gcccatgtgg cgcggcacga cggggtcggc cttttgctgg cacgcgtcgc gacatcggtg
      121 ttctggttcc atccgctggt gtggaagctg gctcacgtcg cgcggcagga gtgtgagcgc
      181 tgctgtgacg acatggtgct cgctgcaggc gagcgagcca cggactatgc cgcgcacctg
      241 ctcgccatcg tgcgctccat gccccgtcag cacgtattcg cggatgtcgc gccggccttg
      301 gctcagggat cgaacctgga gcgtcggttg gcttcgattc tgcgctcggg tcaacgacgc
      361 gattccgtct cgcgggcacg gttcgccggg acgatcgttt tcgcgggcct ggtgctcgcc
      421 gcgaccaccg tcgtccacgt cgtggccgcg ccggaatctg agaatgagat ctgggcctcg
      481 gtcaagaaag ccaaccgcga ggcgcgtcag cacgctcgcg aggtcgaggc cgccatggag
      541 gctgccgcgg ggagcggatc agagatgaag gtgtccgatc ccaaccaaac gggtccctgt
      601 gacgaacctt gcgcccccgt tgctccgagc atcatgtcgg ccccgagcgc catgtcggtg
      661 tccgtcgcgc ccgaggtgtc catggtgccc gaagtgtccg tagtgcccga ggtgtccgtc
      721 gtggtgccgg acgtatccgt gagggtctct ccggtgtttt cggccgtcgg ctcgtcgcac
      781 cgtcgcaagt catacgactc caactcgtca ggcatcgagc tcatgagaga tggtcagtac
      841 tcgcgtgcga tcaccgcgtt ccaggaggaa atccgacaga ccgggagcac gaacgcgatg
      901 tacaacctcg catgcgcgta tgcgctcaag ggggacaaga agctcgcgtt tgatgcactg
      961 cagaagtcga tcgaaaacgg attcgacaat acccaccaca tgacggaaga cgaggacctc
     1021 cgttcactcc agggcgatcc ccacttctat gagctcgttc gattggcccg ggatctgcag
     1081 ctcttcaact cggggcattt cggcggaatg aacggcgaga aggattggag cggtctgctg
     1141 cctcgcctcg agcgcgtcac gcgcgaacac cccggcgtcg gccgtgcctg ggccaacctc
     1201 gggtttgcac gcctggaggc gggtgacgcc aaggggggcg tggccgccta ccagaaggct
     1261 ctcgatctgg gatacgagaa gccgacgact ctatacaacc tagcctgttg cgcggcccgg
     1321 tccggggaca tcgacggggc gttcaagtat ctcgatcgcg cggatcaggc cgggatggag
     1381 atcggcgagc acgtgggttc ggacagcgac ttggacgcgc tccgagggga tccgcgctac
     1441 ggcgccatgc tcaagcgttg ggacgagaag atggcgaagg agcatcgggc caagggtggg
     1501 gcggaggtaa agccacggac tgactgaagc gaaccgcgtg atctcgaccc aaagaaggaa
     1561 gccccaggag acccatcctc ctggggctta acgcattacg ctccttcgta gaagaccccc
     1621 aagcacctcc caattgcgtg acaggtcgat cctccgggga atagactcgt ccggccatgt
     1681 tcccttcccc caccgccaac gcatgctaac gctcagctct gccgcgcgaa tggaagatcg
     1741 ttggggtgac ggccctgcct ccggcgccga cgacctcgca cagctcgtct acctctcgaa
     1801 cctcatcggg caagaaccac gcctcgtaca gccgggcggc ggcaacacgt cgatcaaggt
     1861 cggggatgcg ttgctggtca agggaagcgg caccgacctg cgcacgatcg ggcgggaggg
     1921 attcacacgc ctctccctct cggcactggc cgccctacgc acggtcgaga ccatgctcga
     1981 cgccgacatg atgcgactca tggcgcgcgc gatgctgacg gaaggaccta caccgagcgt
     2041 cgagacaccg cttcactcac tcctcgccgc tcgggtcatc gcgcacactc acgacatagc
     2101 gacgatgagc ctcaccaacg tgagcgatgc cacggcggag cagctcgtca ccgagctgtt
     2161 cgctggagag atcgtgtacg tcccgtacgt gcggcctgga ttcccgctcg cccgaacgat
     2221 cagcgggatc gtggatcggt tgccgctcgg gaccatcggg ctcgccctcg cgcatcacgg
     2281 catcgtggtg tggggggagc aaccgcg
//
LOCUS       DASTKC010000081         8132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_62653, whole genome shotgun sequence.
ACCESSION   DASTKC010000081 DASTKC010000000
VERSION     DASTKC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62653"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2803)
                     /locus_tag="VFR25_03555"
     CDS             complement(<1..2803)
                     /locus_tag="VFR25_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940156.1"
                     /translation="MPRKRWAVLFLASLVTGSSTAPAQSLCPTRKLDDATQILPEAVR
                     PRPALFASFLDSAYATCFERITEDPQIGGARPPVAMGAALQAWNADQSRLLLASGEIL
                     RSPGWAHETSLPAAMAGGAPRWSPIDRDGLFYLEGNALRKLDLFTGSIQTLHVFAEYT
                     SLDAGLGHHDLSRDGRFLVLHGWRGPAGNMEQSDVFVYDLAIDRKGTVLPAVSPITGT
                     LQDAMISPSGAFALVQWSGAGLNPQPMLQAFDSSMVARGRTAPGSGPLDVTVDADGSE
                     WVVRFASGAPGGIQGDYVVKYRLPDGWDRLEGGDATAGDTLCTWQRQVGGGQISGRAF
                     DMGFVIVSADGAPPFATSTRKPFTHEIVKIYLDSSPGAPHLERLADTRSDALFASGSC
                     AAEVAAAARPTATVSRDGTRVLWGSTWDRACRADAYVMDICNKSPNKTLRDLPVNAWV
                     KLSPASYRFDGTREAGDRFPMMQFGRAVFVPEYGSILSWGGGGHGQSRLGNDVWMYDT
                     ARNEWRQMTPGDPLSSYPNPGAATPLLGDYCHNDSSLAVFWSACTPTSNLPIGSTASG
                     APWTSEIYSQRAWDSYNRRFVMFGPNYITGLMHEPEAWFAARASYAYDPFRRTWRFIA
                     NDPHLYHQGGTMAYDPIHRRLVAVNHDWWHHSGYNSSQDRFNFRWWLDVTTDLWTEKP
                     PLLAPNMFDSDLVYDPLTRRMFRQGGDFPTENDLWSYDPENDSWNLLAPAPDPAAGLP
                     PPAAPSAAVNRNGIMLVWGFGGDGECLPTWAYNTQLNSWRKMNALNDPISQAPPTSRV
                     HAWPSLVYDPTNDIFFLLRLSPTEPQTDIGYWPNSLVGEIWAYRYNAPMSITPVPIPE
                     SEPPPKAMLAQNLPNPFRATTSIPFRLDKPARVRLRIFDIHGRVVATLADKTMDGLNE
                     LRWNGKDSRGRDAASGVYWY"
     gene            complement(2957..5419)
                     /gene="leuS"
                     /locus_tag="VFR25_03560"
     CDS             complement(2957..5419)
                     /gene="leuS"
                     /locus_tag="VFR25_03560"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET9940157.1"
                     /translation="MEQRARALWRDERLFEIDDPMKADRRFYCLNMFPYPSGDLHVGH
                     GRNYILGDVVTRLKTMQGYHVLTPMGWDAFGLPAENAAIANKIHPAIWTKRNIARMKE
                     QLISWGLAYDWKREIASCDPSYYRWTQWIFLQLYKKGLAYRAKSAVNWCPKDQTVLAN
                     EQVVDGRCERCGSEVTTRELEQWFFKITAYAEPLLRDLETLTHWPEKVRVMQHNWIGR
                     SEGASIRFALRGEGEPIEVFTTRPDTLYGATFLVLAAEHPRVRELERSGSLSPAATDF
                     VARIRHHRIENRFAVEIEKEGYPTGLEAVHPLTGAPLPVWVANYVLMEYGTGAIMAVP
                     AHDQRDFEFAKKYGLNIVEVIRPESGPGYDGAAAWEGDGRLVNSGPADGTTSAEGKRA
                     VIADLEKRKAGEARVQYRLRDWLISRQRYWGAPIPIVRCPSCGIQPVPESELPVLLPD
                     QVEFSPTGESPLLTNQAFLKATCPKCGGPATRETDTMDTFVDSSWYFLRFLNPALDTA
                     PFSKDAVDRWLPVDQYIGGVEHAILHLLYSRFIVKVLRDMGHLSFSEPFARLFTQGMI
                     TKDGHKMSKSKGNVVAPDALIERFGTDTVRLYTLFIGPPAKDAEWNDRGVEGAYRYLT
                     RYWKMIEDFLQAAGSRTAASSAQHETAWAASGPRRDLRRRTHEIASRLLDDMDRLHLN
                     TAVSGLMQIVNGVQEYQAGGGDLKTPEVEEAVDLATRLMAPLAPHTAEGAWHKLGKPG
                     SVFRSGWPKVSEAALKQDVARYVVSVNGKVRSNIEVPADAGQEVIRERALADGNVRKH
                     TEGKTIQQVIIVPGRLVNVVAQ"
     gene            complement(5590..6687)
                     /locus_tag="VFR25_03565"
     CDS             complement(5590..6687)
                     /locus_tag="VFR25_03565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940158.1"
                     /translation="MSRANATAAVSPTRTLDAPAGLEAFRPEWNAAALERGNLYLTYE
                     WMRSLWESHFGAEGVEFLLHGNDPWSALLPLGFEETRSRGFRLRTALPVTNRYGRNHN
                     DLLLRHSLEAELRWVLTIARERRADLFALASLPDDSPTVPVIRSVASGQGFRVLTEPG
                     AKSPYLTLEGDWDGYLKQQSSNFRSDLKRKGNKARRSALEIRRFTDVASVSMVLEAIY
                     AIETKSWKESTQTSITTNPLAQVFYDRFLHRAAEAGWFESFLLYFEGTPAAYDMGVRF
                     ADRYYMLKTSYDQAWSEQSPGVVLREHVVRHLYETGCREHDFLGDDEGWKMRWTSTVR
                     GHTHYYLYDTSRPKANLYSLLRKLQARFSSP"
     gene            complement(6684..7892)
                     /locus_tag="VFR25_03570"
     CDS             complement(6684..7892)
                     /locus_tag="VFR25_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940159.1"
                     /translation="MAEAARAGATLPPAVILNVYATGLGIARNLAPHGVPVYGLSVQA
                     NAPGNFSRRCHALPCPDSQDEPEALSTFLTRLAARLGARALLLPTRDADIRFLDRHRT
                     ALDPYYEIPQPSGDRLDVIMNKHRLAEEARAAGIPTPLTMRLQSREAARDAATRFTYP
                     VVAKAVYAQDWRKAGIADAVNRRKAVKIASAEELTRFFERVEPHHPDLLVQEWIEGPD
                     DQCVVLGVYRGRGGRVLGWFTARKMLQYPPEFGLGCIVRLEPHPEVERLGKRLLEALA
                     FEGTAEVEFKRHAPSGEYRLIEVNPRHWDQHALGMTCGVNLAWIAYRDLALGDRVDPV
                     APDGRRGAWISGNGLLGSLKADLLQARVRPWKCSDLLHRGTRYAVWDPNDPGPFLGRR
                     IGSAQDPQHR"
     gene            complement(7885..>8132)
                     /locus_tag="VFR25_03575"
     CDS             complement(7885..>8132)
                     /locus_tag="VFR25_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940160.1"
                     /translation="ALEAEGEGGTTALWAVAERALYALDPSPYGYRRSSGPPMRPADA
                     RRALEVVYRADRALKQGELRDAELRDFVEREIAASRG"
BASE COUNT         1330 a         2602 c         2781 g         1419 t
ORIGIN      
        1 cgtaccagta gactccggac gcggcgtcgc ggcctcggct gtcctttccg ttccagcgga
       61 gctcgttcag cccgtccatg gtcttgtcgg ccagagtggc gacgactcgg ccgtggatgt
      121 cgaagattcg aaggcggact cgagcgggct tgtcgaggcg gaacgggatc gacgtggtgg
      181 cccggaacgg gttcgggagg ttttgcgcca gcatggcctt gggcggaggc tctgactctg
      241 ggattgggac cggcgtgatg gacatcgggg cgttgtagcg gtacgcccag atctctccga
      301 cgaggctgtt tggccagtac ccgatgtcgg tctgaggttc ggtcgggctg aggcgcagga
      361 ggaagaagat gtcgttcgtg gggtcgtaga ccagggacgg ccatgcgtgc actcgactgg
      421 tgggcggtgc ctggctgatc gggtcgttca gggcgttcat cttcctccat gagttgagct
      481 gagtgttata ggcccatgtc ggaaggcact caccgtctcc accgaacccc cacaccagca
      541 tgattccatt ccggttcacg gcggccgagg gcgccgccgg aggcggaagg cccgcggccg
      601 gatcgggcgc cggcgcgagc aggttccagg agtcgttctc cgggtcgtag ctccagagat
      661 cgttctcggt cgggaaatca cctccctgcc ggaacatgcg tcgggtgagc gggtcgtaga
      721 ccagatcact gtcgaacatg ttgggtgcga gcaggggcgg cttctccgtc cataggtccg
      781 tcgtgacgtc cagccaccag cggaagttga agcgatcctg gctggagttg taaccggagt
      841 gatgccacca gtcgtgattc acggcgacga gcctccggtg gatcgggtcg tacgccatcg
      901 tgccgccctg gtgatagaga tggggatcgt tggcgatgaa tctccaggtg cgccggaatg
      961 gatcgtacgc atacgaggcg cgcgcggcga accaggcctc gggttcatgc atgagcccgg
     1021 tgatgtaatt gggtccgaac atcacgaaac gccggttgta ggagtcccag gctcgctggc
     1081 tgtagatctc gctggtccag ggtgcgccgg acgcagtgga tccgatcggt agattggacg
     1141 tcggagtgca agctgaccag aacaccgcga gcgagctgtc gttgtggcag tagtcgccga
     1201 ggagcggtgt cgccgccccc ggattcggat agctgctcaa cggatcgccg ggtgtcatct
     1261 ggcgccactc gttccgcgcc gtgtcgtaca tccacacgtc gttgccgagc cgcgactggc
     1321 cgtgtccccc gccgccccag gacagaatcg acccgtactc ggggacgaaa acggcgcgtc
     1381 cgaactgcat catgggaaat cggtccccgg cttccctggt gccgtcgaac cggtaggacg
     1441 ccggagagag cttcacccac gcgttgacgg gcagatctcg aagcgtcttg ttggggctct
     1501 tgttacagat gtccatgacg tacgcgtccg cgcgacacgc gcgatcccac gtgcttcccc
     1561 aaagaacgcg cgtgccgtcg cggctcacgg tcgcagtcgg tcgagcggcg gcggccactt
     1621 ctgcggcgca ggaaccgctc gcgaagagcg cgtcgctccg ggtgtcggcg aggcgctcga
     1681 ggtgcggcgc gccaggcgag ctgtcgaggt agatcttcac gatctcgtgc gtgaacggtt
     1741 ttcgggtgga ggtcgcgaac ggcggggcgc cgtcggcgct cacgatcacg aagcccatgt
     1801 cgaaggcgcg gcccgagatc tgtcccccgc cgacctggcg ctgccaggtg cagagcgtgt
     1861 cgcccgcagt cgcgtcccct ccctcgagcc ggtcccatcc gtccggtagg cggtacttca
     1921 cgacgtagtc cccttggatc cctcccggtg ctcccgaagc gaatcggacg acccactcgg
     1981 agccgtccgc atccaccgtc acatccaagg gtcccgatcc aggagccgtc ctgccgcgag
     2041 ccaccatcga cgaatcgaac gcctgaagca tgggctgggg gttcagccct gcgccggacc
     2101 attgcacgag cgcgaacgcc ccactcgggg agatcatcgc gtcctgcagc gtgccggtga
     2161 tcggagacac cgcgggcagc accgttccct tccgatcgat cgcgaggtcg tagacgaaga
     2221 catcgctctg ctccatgttc cccgcgggac cccgccagcc gtgaaggacg agaaagcgac
     2281 cgtcgcgcga caggtcgtga tggccgaggc ccgcgtcgag cgacgtgtat tccgcgaaga
     2341 cgtgcagggt ctggatcgag ccggtgaaga ggtcgagctt tcggagggcg tttccttcta
     2401 gatagaagag accgtcgcga tcgatgggag accagcgcgg cgccccccct gccatagccg
     2461 cgggaagcga ggtttcatga gcccagccgg ggcttcggag gatctcgccc gaggccagga
     2521 ggagccgcga ctgatcggca ttccaggcct ggagcgccgc gcccatggcg accgggggcc
     2581 gcgcgccccc gatttgagga tcctcggtga ttcgctcgaa gcaggtcgcg taggcggaat
     2641 cgagaaagga tgcgaagagg gcgggacggg gccggacggc ttccgggagg atctgggtcg
     2701 cgtcgtcgag cttgcgggtc gggcagagcg actgggccgg ggcggtggac gaccccgtca
     2761 cgaggctcgc gaggaacaga acggcccacc gctttcgcgg catgcaactc ctacgtggac
     2821 acgtgctctg aagggcgctg ccgccaccgg tggcgtggtc cctggggtgg gatggctgat
     2881 tgacgtgaaa cgatagcaga aattcgtaac ttattcaatt gaaacaataa gcggagactt
     2941 ttgcctcacc ccccccctac tgcgccacta cgttgaccag gcgccctggg acgatgatga
     3001 cctgctggat cgtcttcccc tccgtgtgct ttcgaacgtt cccgtcggct agagcgcgct
     3061 cccgaatgac ctcttggcct gcgtctgccg ggacctcgat gttggaccgg accttcccgt
     3121 tcaccgagac cacgtaccgc gcgacgtcct gcttcagggc cgcctcggag accttgggcc
     3181 agccggaacg aaagaccgag cctggcttcc ctagcttgtg ccaggcccct tcggcggtgt
     3241 gtggcgccag cggggccatc aggcgggtgg cgaggtcgac cgcctcctcg acctcgggcg
     3301 tcttcagatc tccgcctcca gcttgatatt cctgtactcc gttgacgatc tgcatgagcc
     3361 cgctgaccgc ggtattgaga tgcagccgat ccatgtcgtc gagaaggcgc gacgcgatct
     3421 cgtgcgttcg acggcggagg tcgcgacgag gcccggacgc agcccaagcc gtctcgtgct
     3481 gggcggacga cgcggccgtc cggctgccgg cggcctgcag aaagtcctcg atcatcttcc
     3541 agtatcgcgt gaggtagcgg taggcgcctt cgacgccccg atcgttccac tccgcgtcct
     3601 tggcgggagg tccgatgaag agggtgtaga gccgcaccgt gtcggtcccg aacctctcga
     3661 tcaaggcatc gggcgcgacc acgtttccct tcgacttcga catcttgtgc ccgtccttcg
     3721 tgatcatccc ttgcgtgaac aggcgcgcga agggctccga gaagctgaga tgccccatgt
     3781 cgcgtaggac cttcacgatg aatcgtgaat agaggagatg caggatggcg tgctccacgc
     3841 ctcctatgta ctggtccacg ggcagccagc gatccaccgc gtccttcgag aacggcgccg
     3901 tatcgagggc gggattcagg aatcgcagga agtaccagct cgagtcgacg aacgtgtcca
     3961 tcgtgtcggt ctcgcgcgtg gcgggcccac cgcacttggg gcaagtggcc ttgaggaatg
     4021 cctggttcgt gaggagtggc gattcccccg tcggcgagaa ctcgacctgg tcgggcaaga
     4081 gcacgggcag ctccgactcg ggcaccggct ggatcccgca gctcgggcag cgcacgatcg
     4141 ggatcggggc gccccagtag cgctgcctcg agatgagcca gtcccggagg cgatactgga
     4201 cgcgggcctc ccccgccttc cgcttctcga gatcggcgat cacggcgcgc ttcccctccg
     4261 cgctcgtcgt gccgtcggcg gggccggagt tcacgaggcg cccgtccccc tcccacgccg
     4321 cggccccgtc gtacccgggg cccgactccg gccggatcac ctcgacgatg ttcagcccgt
     4381 acttcttcgc gaactcgaaa tcccgctggt cgtgcgcggg cacggccatg atcgcgcccg
     4441 ttccgtactc catgagcacg tagttcgcga cccagaccgg gagcggagcg cctgtcagcg
     4501 ggtgcacggc ttcgaggccg gtcggatagc cctccttctc tatctcgacg gcgaatcggt
     4561 tctcgatccg atggtggcgg atccgtgcca cgaagtcggt ggccgcggga gacagcgagc
     4621 ccgagcgctc cagctcgcgc acgcgcgggt gctccgcggc gagcacgagg aacgtcgcgc
     4681 cgtagagcgt gtcgggtcgc gtggtgaata cctcgatcgg ctctccctcc ccgcgcaggg
     4741 cgaagcggat ggaggcgccc tcgctccggc cgatccagtt gtgctgcatg acgcggacct
     4801 tctcgggcca gtgggtcagc gtctcgagat cccgaaggag cggctcggca taggcggtga
     4861 tcttgaagaa ccattgctcc agctcgcgcg tcgtcacctc ggagccgcag cgctcgcatc
     4921 gtccgtccac cacctgttcg tttgcgagga cggtctggtc cttgggacac cagttgaccg
     4981 cgctcttcgc gcggtaggcg agtcccttct tgtagagctg gaggaagatc cactgcgtcc
     5041 agcgatagta cgaggggtcg caggaggcga tctcgcgctt ccagtcgtag gcaagccccc
     5101 acgagatgag ctgctccttc atgcgggcga tgttgcgctt ggtccagatg gcgggatgga
     5161 tcttgttcgc gatcgccgcg ttctcggcgg gcaggccgaa ggcgtcccat cccatcggcg
     5221 tgaggacgtg gtagccctgc atcgtcttga gccgcgtgac cacgtcgccc aggatgtagt
     5281 tccggccgtg tcccacgtgg agatcgcccg atggatacgg gaacatgttg agacagtaga
     5341 agcggcggtc cgccttcatc ggatcgtcga tctcgaagag ccgctcgtct cgccagagcg
     5401 cgcgggcgcg ctgctccatc tcgcggaacg gatagaaggt tccccgttcc tcggcggatg
     5461 gcgcggcggt cccctcagga tccttggcgt ggctcacggt gtaccctcgt ctcgagatcg
     5521 agatgacgcg tgggaaacgg tcaggatacc ccaaacgtgt agagcggtaa accggagggc
     5581 tgtgcgacct tagggagagc tgaagcgcgc ctggagcttc ctcaagaggg agtagagatt
     5641 cgccttgggg cgcgaggtgt cgtacaggta gtagtgagtg tggccgcgga ccgtggacgt
     5701 ccagcgcatc ttccacccct catcgtcccc caggaaatca tgctcgcggc accccgtctc
     5761 gtagagatgg cggacgacgt gctcacgaag cacgaccccc ggactctgtt cgctccaggc
     5821 ctggtcgtag ctcgtcttca gcatgtagta ccggtccgcg aatcgcacac ccatgtcgta
     5881 cgccgccgga gtcccttcga agtagaggag gaacgactcg aaccagccgg cctcggccgc
     5941 gcgatgcagg aatcggtcgt agaacacctg ggccagtgga ttcgtggtga tcgaagtctg
     6001 ggtggattcc ttccagctct tggtctcgat ggcgtagatg gcctcgagca ccatggacac
     6061 ggaagcgaca tcggtgaagc gccggatctc gagggcggac cgtcgggctt tgtttccctt
     6121 ccgcttgagg tcgctccgga aattcgagga ctgctgtttc aggtagccgt cccagtcccc
     6181 ttccagcgtg agatacggag acttcgcgcc gggctcggtg agaacgcgaa atccctgacc
     6241 ggaggcgacg cttcgaatca cagggaccgt cggactgtcg tccgggagcg atgcgagggc
     6301 aaagagatcg gcccggcgct cccgtgcgat ggtgagcacc catcggagct ccgcctccag
     6361 agaatggcgc aggagcagat cgttgtgatt ccgcccatat cgattggtca caggcagggc
     6421 tgtgcgcagt cggaagccgc ggcttcgggt ctcctcgaat ccgaggggca agagcgcgct
     6481 ccacggatcg ttcccgtgga gcaggaactc cactccctcg gcgccgaagt ggctctccca
     6541 gagcgacctc atccactcgt aggtgagata gagatttcca cgctccagag cggccgcgtt
     6601 ccactccggg cggaaggcct cgagccccgc gggtgcatcg agagtgcgcg ttgggctcac
     6661 cgcggcagtc gcgttcgcgc ggctcatcgg tgctgtggat cctgggcgga accgatacgg
     6721 cgtccgagga acggaccggg atcgttggga tcccacacgg cgtagcgagt tccccggtga
     6781 aggagatcgg agcacttcca gggcctcacg cgcgcctgga gcaggtccgc cttcaaggag
     6841 cccagaaggc cgttgccgct gatccaggct ccacgtcgcc catccggtgc gaccggatcc
     6901 acgcgatcgc cgagggcgag gtcgcgatac gcgatccacg cgagattgac gccgcacgtc
     6961 attccgagcg cgtgctggtc ccagtggcgt ggattcacct cgatcaggcg gtactccccg
     7021 ctcggcgcgt gccgcttgaa ctcgacctcc gccgttccct cgaaggcgag cgcttcgagg
     7081 agccgctttc ccaggcgctc cacctcgggg tgcggctcca gccgcacgat gcagccgagg
     7141 ccgaactcgg gcggatactg gagcatcttc cgggcggtga accatccgag cacgcggcct
     7201 cctcgcccgc gatacacgcc gagcacgacg cactggtcgt ccggcccctc gatccattcc
     7261 tggaccagga gatcgggatg atgcggctcg acccgctcga agaatcgcgt cagttcctcc
     7321 gcggaagcga tcttcacggc cttgcggcgg ttcaccgcgt ccgcgatgcc cgccttgcgc
     7381 cagtcctggg cgtacaccgc cttggcgacg accggatagg tgaaccgtgt cgcggcatcg
     7441 cgtgccgcct cgcgcgattg caggcgcatc gtgagcggcg tgggaatgcc cgcggcgcgc
     7501 gcctcctcgg ccagccggtg cttgttcatg atcacgtcca ggcgatcgcc gctcggctgc
     7561 gggatctcgt agtagggatc gagagcggtg cggtgccggt cgaggaagcg gatgtccgcg
     7621 tcgcgcgtcg gaagcaggag ggcgcgagcg ccgagacgcg ccgcgagacg cgtgagaaac
     7681 gtggacagcg cctccggctc gtcctgcgaa tcggggcacg gaagcgcgtg acagcgcctc
     7741 gaaaagtttc ccggggcgtt ggcttgaacg ctgagaccgt acacgggtac accgtggggc
     7801 gcgaggttgc gcgcgatgcc gaggccggtg gcgtacacgt tgaggatgac ggcgggagga
     7861 agcgtggcgc ccgctcgcgc ggcctcagcc acgggaagcg gcgatctccc gctccacgaa
     7921 gtccctgagc tccgcatcgc ggagctcgcc ctgtttgagt gcgcgatccg cgcgatagac
     7981 cacctcgagg gcgcgtcgcg catcggcggg acgcatgggc ggtccggagc tccggcggta
     8041 gccgtaggga gacggatcga gcgcgtagag ggcgcgctcc gccaccgccc agagcgcggt
     8101 ggttccccct tctccctccg cctcgagcgc gg
//
LOCUS       DASTKC010000082         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1246442, whole genome shotgun sequence.
ACCESSION   DASTKC010000082 DASTKC010000000
VERSION     DASTKC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246442"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /locus_tag="VFR25_03580"
     CDS             complement(<1..2156)
                     /locus_tag="VFR25_03580"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET9940161.1"
                     /translation="MTLAIVLILGLSGLFTLVVAGVLLAFRSDMRELQMRIERLELRG
                     ITPPPEPVRTPEPAVMPPTRLTPPVAVAPPTPSAPATPALVETPPPPTPAPAAAPTPA
                     PAPSFQSVHSRGEAPRLDRGRLEQQIGGIWMQNVGSVLLLLGAFFLIVWGYANKRIGP
                     EILVLAGVALGVVVAWRGHVIGRTLVALGNAIIGIGLGVVYITLYLGHFRMHVFQGAV
                     AFSLLTLTSLVSVGIGLRRREPIITTLGTLAAYLPQLLAVWIPLQGFRLALPALLGYF
                     AVVNVVVFALAASVGWSGLVLLSLVLTTVTWGANSGLVPWGLGIQLGLSALYVALGLA
                     PVVRLARDEKRVRGIDIAVITAAPMLLLIASFPFLVAPWRMRSGLLLTGLSAVYLGIS
                     LWVEKRRREQDLWRPLTAASTVFLAAGIERGLLPEYLALAWCAQGMALLWLGLGERGA
                     WFRRLGYAILAVAATRLFFVLVTNQPDSLLGAPAIRDLIVILALVLASHLLGTARDRL
                     DPEERYAYGPCALIANLLLMIWIGREASQLKHVLPQLARPDLGFVRLALTSACWMIQC
                     VVLLYLSIVRGGAVLRYIGYVVGACASVGILISLAGADWRPEFMTLLNTTAIVIAFAT
                     TLVILGAEFLARHRDRLGPSERVMPEVATVIGNFILMIWIGRQADHLRYVLPQVSWSY
                     YGPGFVTLTLASACWMVQCVVLLYLSIARNAPVLRY"
     gene            complement(2153..>2672)
                     /locus_tag="VFR25_03585"
     CDS             complement(2153..>2672)
                     /locus_tag="VFR25_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HET9940162.1"
                     /translation="KALAENAMAQLEDAQLAVSAGTATESNSVAVLVWHVSGNLTSRF
                     TDFLTSDGEKPWRQREEEFAARTVSRAELMAKWNQGWDALLETLTALTDADLSKSVTI
                     RGQSLLVHEALHRSLAHTCYHVGQIVYVAHAIRGREWRYLSIAPGQSDAYNANPTDET
                     AQAHAERLKERT"
BASE COUNT          523 a          836 c          951 g          362 t
ORIGIN      
        1 atgtatcgaa gcacgggagc attgcgggcg atggagaggt agaggagaac cacgcactgg
       61 accatccagc acgccgacgc gagcgtgagc gtgacgaacc ccgggccgta gtaggaccaa
      121 gacacttggg gcaggacgta gcggagatga tccgcctgtc tcccgatcca gatcatgagg
      181 atgaagttgc cgatgaccgt ggcgacctcg ggcatgacac gctcggaagg tccgagccgg
      241 tcccgatggc gcgcgaggaa ctcggcgccc aggatcacga gcgtcgtggc gaacgcgatc
      301 acgatcgccg tcgtgttgag gagggtcatg aattccgggc gccaatcggc gccggccagg
      361 ctgatgagga tcccgacgga ggcgcaggcg ccgactacgt aaccgatgta ccgaaggacg
      421 gcaccgccgc ggacgatcga gaggtagagg aggaccacgc actggatcat ccagcacgcc
      481 gacgtgaggg cgagcctgac gaaacccagg tcgggtcggg cgagctgtgg aagcacgtgc
      541 ttgagctggc tcgcttcccg ccctatccag atcatgagaa gcaggttcgc gatgagcgca
      601 cacggcccgt atgcgtagcg ttcctcggga tccagacggt cccgcgcggt cccgagcaga
      661 tgggacgcaa ggacgagagc gagaatcacg atcagatcac gaatcgcggg cgcgcccagg
      721 aggctatcgg gctgattggt gacaagaacg aagaagagtc gggtcgcggc gacggcgagg
      781 atcgcgtatc ccaagcgccg gaaccacgcg ccccgctctc caaggccgag ccagaggagc
      841 gccatgccct gcgcgcacca tgcgagcgcc aggtactcgg gaagaaggcc gcgctcgatt
      901 ccggcggcga ggaacacggt ggaggcggcg gtgagcgggc gccagagatc ctgctcccgc
      961 cgacgcttct cgacccacag ggagatgccc aggtagaccg cagaaagtcc cgtgagcaga
     1021 agcccggagc gcatccgcca tggcgcgacg aggaagggga atgaggcgat gagaaggagc
     1081 atcggagccg cggtgatgac ggcgatgtcg atcccgcgaa cgcgtttctc atcgcgcgcc
     1141 agcctgacga ccggggccaa acccaatgct acgtagagag cggacaggcc gagctggatc
     1201 ccgagtcccc aaggaaccag ccccgagttg gcgccccagg tcaccgttgt gagcacgagc
     1261 gacaggagca cgagcccgct ccagccgacc gaggccgcga gcgcgaagac gaccacgttc
     1321 accaccgcga aatatccgag gagcgcgggg agcgcgagcc gaaagccctg gagcggaatc
     1381 cacaccgcga ggagctgggg gagatacgcc gcgagggtcc cgagggtcgt gatgatgggc
     1441 tcgcggcggc ggaggccgat cccaaccgag acgagcgacg tgagcgtgag gagcgagaac
     1501 gcgacagcac cctggaacac atgcatccgg aagtggccca ggtagagcgt gatgtagacg
     1561 acgccaaggc cgattccgat gatcgcgttc ccgagcgcta ccagcgttcg tccgatcacg
     1621 tgcccgcgcc aggccacgac gactccgaga gcgacccccg cgagcaccag gatctccggg
     1681 ccgatccgct tgttggcgta gccccacacg atcaggaaga aggctccgag caggagcagc
     1741 accgatccca cgttctgcat ccagatcccg ccgatctgct gttcgagccg tccgcggtcg
     1801 aggcgcggcg cttcgccgcg ggagtggacc gattggaagc ttggggccgg cgcgggcgtt
     1861 ggcgcggctg cgggagccgg ggtcggtggc ggaggtgtct cgacgagcgc aggtgtcgcg
     1921 ggcgcgctcg gcgtaggagg tgccacggcg acgggtgggg tcaaccgtgt cggtggcatc
     1981 accgccggct cgggcgtccg aacgggttcc ggcggcggag tgattccgcg aagctcgagc
     2041 cgttcgatcc gcatctggag ctcacgcatg tccgagcgga acgcgagcag cacgccggca
     2101 accacgagag tgaacaggcc cgagagtccc aggatcagga cgatcgccag agtcatgtcc
     2161 tctccttgag gcgttcggca tgcgcctggg ccgtctcgtc ggtcgggttc gcgttgtatg
     2221 cgtccgattg ccccggagcg atgctgagat accgccattc gcgtccacga atcgcgtgcg
     2281 ccacgtagac gatctgcccc acgtgatagc acgtgtgcgc gagcgagcga tggagcgcct
     2341 cgtgcaccag gagcgactga ccgcggatcg tgaccgactt cgagaggtcg gcgtcggtga
     2401 gggcggtcag ggtttcgagg agagcatccc acccctgatt ccacttcgcc atgagctcgg
     2461 cgcgcgagac cgtgcgggcc gcgaactcct cctcgcgctg ccgccacggc ttctcgccgt
     2521 cggacgtgag gaagtcggtg aagcgcgacg tgagattccc cgagacgtgc cagacgagca
     2581 ccgcgaccga gttcgactcg gtcgccgtcc ccgccgaaac cgcgagctgc gcatcttcaa
     2641 gttgcgccat cgcgttttcg gccagcgctt tg
//
LOCUS       DASTKC010000083         7581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_536496, whole genome shotgun sequence.
ACCESSION   DASTKC010000083 DASTKC010000000
VERSION     DASTKC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536496"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2547)
                     /locus_tag="VFR25_03590"
     CDS             complement(<1..2547)
                     /locus_tag="VFR25_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940163.1"
                     /translation="MLLIAWLAAALPASAQDEFLVNDDRVNKNQWAPTVACGSSGSLV
                     VIWMDGRNTFNSNVDFDSYALTIHDPKGIGTSLNRRINDDSNGGTQSFPDIAASPAGT
                     FFCVWEDTRAANRDIYGAALDSIGLRITPNLRLNDDVGSFDQSHPAVTAVGPDRYLVV
                     WADQRQAQGEIFGVYVTAAGAPIGPNFKISVDPVVGGSSQGGPAVAARPDGSVLVAWQ
                     DGRGGATIGATDDVFAQRLNALGQPVGGNFKVNGTTGNQDDESVCVAADASGFVVGWI
                     DRRDKPTDTGDVYAQRIDPSGGLVGGNVRVNDDPLGKDQRAVRAFSAPGVAYLLWEDY
                     RGNLGIDANVQAARVPYDTTAPGANFRVNAFLPARQGTPAAVWDGRDGIVAVWEDGRN
                     GSPDVYALSILPDGTRRNSETQLNDDAASGDQRRPHVGRGPGQYLATWIDYRTGTGDL
                     YGQWITASGGRSGANHLIARDDGVNRPVTSSSAVTASGAGLIAAQLTRDSDAGEIRGF
                     LYAAPGTPPVSGFWISDRLASAQSSPATTTDGVTFAVAWLDARTGTTQPFGQLLTLAG
                     ARIGDNHPILTVPPSSPVVGVDLDGDGSGGYWLAYAIDGVAEPGLWIVHLGSDLRQDR
                     ATVEVGAGQTGTRSEPHIGLGSEGRIEVAWLCSDASGLPRVYVQSFATSGVALSNPIR
                     LDPGHDDAMLSPGIAVSGGQSVVTWESKRVGDWTPWMQAFEGGVAPSTGILRVDQDVL
                     GADQLDPAVGLDASGHVVVLWSDTRSVSSGADIVGRVFAITPTPVRDPDPPPAPDPLP
                     APLAFRIGASPNPFSGALAIPIQRPGAAGWHLRVRVVNVRGELIRTLYDGP"
     gene            complement(2622..3998)
                     /locus_tag="VFR25_03595"
     CDS             complement(2622..3998)
                     /locus_tag="VFR25_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET9940164.1"
                     /translation="MNPQIFREYDIRGIADRDLTDDVARAVGRAFAQMMAERGKRRIA
                     VGRDVRLSSPRIRAAVVEGLRDQGASVVDVGQVPTPALYFAVLHLKAEAGVMITGSHN
                     PIEYNGIKVSEGTSSLHGPEIQEIARRGATAGVLEKRGTVEEVSVDEAYMADLSGRIK
                     LAKPLKVVCDPGNGAASILGPRFLRAIGCDVDAMFAEPDGRFPNHLPDPTVPELMKPL
                     CDRVRATKADVGIGFDGDADRVGAVDENGRMIFGDELLGLFAADVLAKKPGSTVVFEV
                     KCSQGLVDWVKQKGGNPIMWKAGHSLIKAKMRETHAPLGGEMSGHMFFADEFYGYDDA
                     LYGAGRLLRILASAGRPLSALVKELPQSQYVSTPELRLDCTDAAKFAIVDSVREHYRP
                     RYDVLDVDGARVQFGDGWGLIRASNTQPVLVVRFEAKSRERLQEIARDVFDTLSRFPE
                     VTIPALTV"
     gene            complement(3995..5203)
                     /gene="prfB"
                     /locus_tag="VFR25_03600"
                     /pseudo
     CDS             complement(3995..5203)
                     /gene="prfB"
                     /locus_tag="VFR25_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893533.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(5180..5713)
                     /gene="coaE"
                     /locus_tag="VFR25_03605"
                     /pseudo
     CDS             complement(5180..5713)
                     /gene="coaE"
                     /locus_tag="VFR25_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis.; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
     gene            complement(5761..>7581)
                     /gene="polA"
                     /locus_tag="VFR25_03610"
     CDS             complement(5761..>7581)
                     /gene="polA"
                     /locus_tag="VFR25_03610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET9940165.1"
                     /translation="VLEPPREPFGSYTIVKDAEGLEALARELSETSGPVAFDTETTGF
                     DPHRSDLVGVALATAPGRAFYLPVGHRQGTVLDKDHVREALRPFFDSTDGWRVAQNAK
                     FDWHVLHAFGIPVKDVAFDTMIAGFLVDPDQPKNLDALSWSRLGLKKITTESVIGSGR
                     DQISMADAPIERVAEYCCEDADACLRLVPLLTHELEAAEALPLFRDVEMPLVGVLTRM
                     ERAGVKVDVSQLEGMAKDMGAELQRLDHEIQELAGVPFNVSSPRQVAEVLFERLKLPR
                     GKRTKDGYSTDAEVLEALAAVHPLPKLLLAYRQVEKLKSGYIDTLPRLTDKDTGRVHA
                     TFHQTVASTGRLSASDPNLQNIPIRTAEGSAIRRAFVAEGERGLLASFDYSQIELRLL
                     AHFSHDPVLTEAFRTGEDVHATTAAKLFGVAPDQVTSAQRAQAKIVNFGILYGMGPVR
                     LARELNLSRPLAAAFIEEYKRTLAGVAQYIERTLAQARRTGYAETILKRRRPFANLRS
                     EGARRAEAERAAVNMPIQGSAADLIKIAMVRIDALLAERKCRTRLILQVHDELLFETD
                     PEELAEIGPLIRQVMEHAIPLRVPLVVHEGSGANWADAHP"
BASE COUNT         1165 a         2659 c         2540 g         1217 t
ORIGIN      
        1 cggtccgtcg tacaaggtcc ggatcaattc cccccgcacg ttgaccacgc ggacgcgaag
       61 atgccacccc gccgcgcctg gccgctggat gggaatcgcg agcgccccgg agaacggatt
      121 cggcgaagct ccgatgcgga aggcgagcgg agcgggcagc ggatccggcg ccggcggcgg
      181 gtcagggtcc ctgaccggcg tcggggtgat cgcgaacacg cgcccgacga tgtccgcccc
      241 gctcgagacc gagcgagtgt cgctccacag cacgaccaca tggccggatg catcgaggcc
      301 gacggcagga tcgagttggt ccgcgccgag cacgtcctga tcgacccgca agatccccgt
      361 cgagggcgcc acgcctccct cgaaggcctg catccacgga gtccagtcgc cgacccgctt
      421 ggactcccac gtcacgaccg actggccgcc ggacacggcg atccccggag atagcatggc
      481 gtcgtcatgg cctgggtcga gccggatcgg gttcgagagc gcgactcccg aggtggcgaa
      541 gctctgcacg tagacgcgcg ggagccccga tgcatcgctg cagagccacg ccacctcgat
      601 ccgcccttcc gagcccaggc cgatgtgcgg ttcggatcgc gtcccggtct gcccggctcc
      661 gacctcgacc gtggcccggt cctggcgcag atccgagccc aggtgcacga tccagagccc
      721 gggttccgcc acgccgtcga tcgcataggc gagccagtag ccgcccgagc cgtcgccatc
      781 gaggtccacc cccacgaccg gcgatgaagg gggcacggtg aggatcggat gattgtcccc
      841 gatcctcgct cccgccagcg tcaggagctg accgaagggc tgggtcgtcc ccgtgcgcgc
      901 gtcgagccag gccaccgcga acgtgacgcc gtcggtcgtc gtcgccgggc tcgattgcgc
      961 gctcgcgagg cggtccgaga tccagaagcc cgagaccggg ggggtgccgg gcgcggcata
     1021 caggaagccg cgaatctcgc ccgcgtccga atcgcgtgtc agctgtgccg cgatgagacc
     1081 ggcgccggac gcggtcacag ccgacgacga ggtgacggga cggttcacgc cgtcatccct
     1141 cgcgatgaga tggtttgcgc ccgatcgtcc gcccgacgcc gtgatccact ggccgtagag
     1201 gtctccggtt ccggtcctgt agtcgatcca cgtggccagg tactgccctg gtccgcgccc
     1261 gacatgaggg cgcctctgat cccccgacgc ggcatcgtcg ttcagctgcg tctccgaatt
     1321 gcgccgcgtg ccgtcgggca ggatggagag ggcgtagacg tccggcgagc cgttccgccc
     1381 gtcctcccac acggccacga tcccgtcgcg gccgtcccac accgcggcgg gcgtgccctg
     1441 gcgcgccgga aggaacgcgt tcacgcggaa gttcgccccg ggcgcggtgg tgtcgtaggg
     1501 gacgcgcgcc gcttggacgt tggcgtcgat gcccagattg ccccgatagt cctcccagag
     1561 caggtacgcg acacccgggg ccgagaaggc gcgcacggcg cgctgatcct tgcccagggg
     1621 atcgtcgttc acccgcacgt tgccgccgac aaggccgccg gatgggtcga tgcgctgcgc
     1681 gtacacgtcg ccggtgtccg tgggcttgtc gcgccggtcg atccagccga cgacgaagcc
     1741 cgacgcatcg gccgcgacgc agaccgattc gtcatcctgg ttcccggtgg ttccattgac
     1801 cttgaaattg ccgccgaccg gctggccgag cgcgttcaag cgctgcgcga acacgtcgtc
     1861 cgtcgcgccg atcgtggcgc cgccgcggcc gtcctgccac gcgaccagga cggagccgtc
     1921 gggccgcgcc gccaccgcgg gtccgccctg ggagctgcct cccacgaccg ggtccaccga
     1981 gatcttgaag ttgggtccga tgggggcgcc cgcggcggtg acgtagacgc cgaagatctc
     2041 gccctgggcc tggcgctgat cggcccacac gacgagatag cgatccggac cgacggccgt
     2101 gacggcagga tgcgactgat cgaaggagcc gacgtcgtcg ttcaagcgga gattgggcgt
     2161 gatccggagt ccgatggagt cgagcgccgc accgtagatg tcgcgattgg ccgcgcgcgt
     2221 gtcctcccag acgcagaaga aggttccggc cgggctcgcc gcaatgtcgg gaaagctctg
     2281 cgtgccgcca ttcgagtcgt cgttgatgcg ccggttcaag gaggtcccga tccctttcgg
     2341 atcgtggatc gtgagggcgt acgagtcgaa gtccacgttc gagttgaacg tgttccgtcc
     2401 atccatccag atcacgacga gcgacccgga cgagccgcag gccacggtcg gcgcccactg
     2461 gttcttgttc acccggtcgt cattgacgag gaactcgtcc tgggcgctcg cgggcagagc
     2521 cgcggccagc caggcgatga ggagcaacgg cagaacgccg ggacgcaaag gcaacgagcg
     2581 atcacccttt ccggcgtggc gcgagaccgg cggcgctccg atcagactgt gagcgcgggg
     2641 atcgtcacct cggggaatct ggacagggta tcgaagacgt cgcgggcgat ttcctggagc
     2701 ctctcgcgcg acttcgcctc gaaccggacc accagaaccg gctgggtgtt ggatgcgcgg
     2761 atgagccccc agccgtcccc gaactggacc cgggcgccgt ccacgtcgag gacgtcgtac
     2821 cggggcctgt agtgctcgcg aacggagtcc acgatagcaa acttggccgc gtcggtgcaa
     2881 tcgagtcgaa gctcgggggt ggagacgtac tgggattgcg gcaactcctt gaccagcgca
     2941 gagagcggac ggccggccga agcgaggatg cgcaggagcc ggcccgcccc gtagagcgca
     3001 tcgtcatacc catagaactc gtccgcgaag aacatgtgcc ccgacatctc gccgcccagg
     3061 ggggcgtggg tttcacgcat cttcgccttg atgagagaat gacccgcctt ccacatgatc
     3121 gggtttccac ccttctgctt cacccagtcg acgagcccct gggagcactt cacctcgaag
     3181 acgaccgtgc ttcccggctt cttcgcgagc acgtccgccg cgaagagccc gaggagctcg
     3241 tccccgaaga tcatccgccc gttctcgtcc acggcgccca cgcgatcggc gtcgccgtcg
     3301 aatccgatcc caacgtccgc cttcgtggcg cgcacccggt cgcacagggg cttcatcagc
     3361 tccggcacgg tcgggtccgg gaggtggttc gggaaccgtc cgtcgggctc ggcaaacatg
     3421 gcgtccacgt cgcagccgat ggcccggagg aagcggggtc ccaggatgct ggcggcgccg
     3481 ttccctgggt cgcacacgac cttgagcggc ttcgcgagct tgatccgacc gctcaggtcg
     3541 gccatgtagg cctcgtcgac ggagacctcc tcgacggtgc cgcgcttctc gagaacaccc
     3601 gcggtcgcgc cgcgccgcgc gatctcctgg atctcgggac cgtggagcga gctggtcccc
     3661 tccgaaacct tgatgccgtt gtactcgatc gggttgtggc ttcccgtgat catcacgccg
     3721 gcctccgcct tcagatggag cacggcgaag tagagcgcgg gcgtcggcac ctgcccgacg
     3781 tccacgaccg aggctccctg atcgcgaaga ccctcgacca ccgccgcgcg gatgcgcggc
     3841 gacgagagcc ggacgtcgcg gccgacggcg atgcggcgct tgccgcgctc cgccatcatc
     3901 tgcgcgaacg cgcgccccac ggcgcgcgcg acgtcgtcgg tcaggtcgcg gtcggcgatg
     3961 ccgcggatgt cgtactcgcg gaagatctgc gggttcacgg gcgctccttc attctgaggt
     4021 aggcatcgat gaatggatcg aggtctccgt ccatcacggc ctgcacgtcg gtgacgacga
     4081 cgtcggtgcg atggtccttg acctgagtgt acggctgaaa gacgtacgag cggatctgat
     4141 tcccccaggc gatgtccttc ttgccaccac cgagcgagtc gcgcttctcc ttgtcctcct
     4201 cgagccgcat ggagtagagg cgcgcggtga ggaccttgag cgccgcgtcc cggttcctgt
     4261 gctgcgaccg ctcgctctgg cactggacga cgagcccggt cggcaagtgc gtgatccgca
     4321 ccgccgactc ggtcttgttc acgtgctgcc ctcccgcgcc gctcgcgcgg taggtgtcga
     4381 tacggaggtc gccatccttg atctcgacgg cgccgtcgtc ccccgcctga ggatagatga
     4441 agacggatgc gaacgacgtg tgtcgacgct tctgagcatc gtagggggag atgcgcacca
     4501 gccggtgcac gccgcgctcg gctttgagat agccgtacgc gaggtcgcca tcgatctgaa
     4561 tcgtggcgtc cttgatcccc gcctcgtccc ccgcctgctg gtcgagcacc tgaaccttcc
     4621 agccccgccg ctcgacatag cggaggtaca tgcgaaggag catctccgcc cagtcctggg
     4681 actcggtccc gccggcgccc ggatgaatcg tgaggatggc gccgtgcgcg tcgctctcct
     4741 ccgagaggag gctccggatc tcgaggtcct ggatgcgcgg aaggagccgc ttcgactgct
     4801 cctcgagctc ctgcgccagc gcctcgtccg gatcgtcggt caggagcgcg gccatgcctt
     4861 cggcgtcgtc cacttcgcgg gcgagctcgc gatagggctc ggtcacccgc ttgagcgatt
     4921 tcagctcggt gaccgtcctc tgcgcctgat ctccgtcgtc ccagaaaccg ggagcggcca
     4981 tccgctcctc cagctccttg gcccgcgttt cgcgctgatc gacgtcaaag atagctcccg
     5041 agctgcgcca ggatctggcg tccatcctcg agcctctttc gcgtagcact ccagtccatc
     5101 gatgctcctt ccgtcaggcc ttcgcttcgg acaggctttg gatctcatcc cagatcgcgt
     5161 ccgcctgcgc gacgagcgcg gcgcgatccc ccgcattctc caccttccag agccgcctcg
     5221 ctccgcgcac aggcgggagc ttctgacctt ggatgcgccc tcgcgcctcg aggtcggaga
     5281 aggatcgcgc cgcgcggagc ctccgcgccc gctcatcctc cgacgcgacc acctcgacca
     5341 cgccgtcgag ctcgggctcg agccgccacg tcgtgagcat ggccgcatcg agcacgcgca
     5401 cgagggcgcc gggtgcttcg agcgcggcgc gcacccgctc gaggagcttc ggctgcacga
     5461 tcgcgttcag ccggcccatc cggtccggat ccccgaagac gatcttcccc agccgcgctc
     5521 gatcgacctc gccttgccgg tccatcacgt cctcgccgaa cgcctggcgg atcgccgcgc
     5581 gcacctcggc caggcgcaag acctcccagc cgagggcgtc gccgtcgacg agatcggcgc
     5641 ctcgctcccg gaaccggcgc gccagggtcg atttgccgga ggccatcgcc cccgtgacgc
     5701 cgatccggac catcacgtca acctatcggc gcgcgcgcgc gagggtcaag ccgaaagccc
     5761 tcatggatgc gcgtcggccc agttcgcccc gctcccctcg tggaccacga gcggcacgcg
     5821 gagcggtatg gcgtgctcca tcacctggcg gatcaagggg ccgatctcgg cgagctcctc
     5881 cggatcggtc tcgaagagga gctcgtcatg cacctggagg atgagccgcg tgcggcactt
     5941 gcgctccgcg aggagggcgt cgatccgcac catcgcgatc ttgatgagat ccgcggccga
     6001 gccctggatg ggcatgttga ccgcggcgcg ctccgcttcg gcccggcgcg ccccttccga
     6061 tctcagattt gcaaacgggc ggcgccgctt gagaatcgtt tccgcgtagc ccgtgcgccg
     6121 cgcctgggcg agagtccgct cgatgtactg cgcgacgccg gcgagcgtcc gcttgtactc
     6181 ctcgatgaac gccgccgcga gcggacgcga gaggttgagc tcccgcgcga gccgcaccgg
     6241 ccccatgccg tacagaatgc cgaaattcac gatcttcgcc tgcgcacgct gcgcggatgt
     6301 gacctggtcg ggcgccacgc cgaagagctt cgcggccgtc gtggcatgca cgtcttcccc
     6361 ggtccggaac gcttcggtga gcaccggatc gtgcgagaag tgggcgagga gccggagctc
     6421 gatctgcgag tagtcgaagg aggcgaggag accgcgctct ccctccgcga cgaacgcgcg
     6481 gcggatcgcg ctcccctccg ccgtccggat ggggatgttc tggagattgg gatcgctcgc
     6541 gctcaagcgc cccgtcgacg ccacggtctg gtggaaggtg gcatgcaccc ggccggtgtc
     6601 cttatccgtg aggcgcggca gggtgtcgat gtagccgctc ttcaacttct cgacctggcg
     6661 atacgcgagg agcagcttgg gaagcggatg caccgccgcg agcgcctcga gcacctcggc
     6721 atcggtcgag tagccatcct tcgtgcgctt gccgcgcgga agcttgagcc gctcgaacaa
     6781 gacctcagcc acctggcgcg gcgaggagac gttgaacggc acgccggcga gctcctggat
     6841 ctcgtgatcg agacgctgga gctccgctcc catgtccttc gccatgcctt cgagctggct
     6901 gacgtcgacc ttcaccccgg cgcgctccat gcgcgtgagc acgccgacga gcggcatctc
     6961 cacgtcgcgg aagagcggaa gcgcttcggc ggcctcgagc tcgtgggtca ggagcggaac
     7021 gaggcggaga cacgcgtccg cgtcctcgca gcagtattcc gccacgcgct cgatgggcgc
     7081 atcggccatg ctgatctgat cgcgccccga tccgatcacg gactccgtcg tgatcttctt
     7141 cagtccgaga cggctccacg agagtgcgtc caagttcttc ggctggtcgg gatcgacgag
     7201 gaagcccgcg atcatcgtgt cgaacgcgac gtccttgacc gggatcccga acgcgtggag
     7261 gacgtgccag tcgaacttcg cgttctgcgc gacgcgccag ccgtcggtcg aatcgaagaa
     7321 gggacgaagc gcctcgcgca cgtggtcctt gtcgagcacc gtcccctgcc ggtgaccgac
     7381 cgggaggtag aacgcgcgcc ccggcgccgt cgcgagggcg acgccgacca agtcggagcg
     7441 atgcggatcg aatcccgtgg tctcggtgtc gaaggcaacc ggtcccgagg tttcggagag
     7501 ctcccgcgcg agcgcttcga gcccttctgc gtccttcacg atcgtgtagc tcccgaatgg
     7561 ctcgcgcggc ggctcgagaa c
//
LOCUS       DASTKC010000084         8310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_773804, whole genome shotgun sequence.
ACCESSION   DASTKC010000084 DASTKC010000000
VERSION     DASTKC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773804"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFR25_03615"
     CDS             complement(<1..188)
                     /locus_tag="VFR25_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9940166.1"
                     /translation="MTEGWKERGRSWFRPIGAALAKRGVKPDHLTIAGLGLSLLAALL
                     LARGDFLSAAVVLGLSGL"
     gene            complement(185..940)
                     /gene="rsmI"
                     /locus_tag="VFR25_03620"
     CDS             complement(185..940)
                     /gene="rsmI"
                     /locus_tag="VFR25_03620"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9940167.1"
                     /translation="MSGEPEPGTLYLVATPIGNVEDLSPRARHILAEAGVVAAEDTRH
                     TRGLLARFGIDRALVSYHDHNKDARTPELVGRLREGMSVAVVSDAGSPGISDPAFTLV
                     RAAVREDLRVVPIPGPSSVLCALEVSGLPTDRFAFEGFLPRKPGRRRTRIRELREDPR
                     TLIFFESPHRVAAALRDLMAELGDRPASISRELTKKFEETRRGPLSSLLAWVGAKTPR
                     GEFVLVVGGAPKDGDDREEAEEPPPRDEWEERE"
     gene            complement(937..1998)
                     /locus_tag="VFR25_03625"
     CDS             complement(937..1998)
                     /locus_tag="VFR25_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET9940168.1"
                     /translation="MTTAYVDLRSDTVTRPTAAMRRAMAEADVGDDHFGEDPTVRLLE
                     EETARLLGKESALFVPSGSMGNQIAIGIQTRRGDQVLLHERSHVAYSEEAALTSFLGL
                     RLLPVVGDAGRLAPESIAAALDSAAREGNGRVGLVELENSHNWAGGAVYDVERLGRAA
                     RPARERGLPVHLDGARLWNASAATGTPVSEFADAADTVMVCYSKGLGAPVGSAIAGPK
                     ALIGEARTLRKRFGGGMRQAGILAAGALHGLRHHRERLVDDHGRARRLAEALASVPGL
                     TIDPKTVTTNIVIVEVTPHERVQELADRAAEAGVRLLVFGGPGRFRAATHLDVDDPAI
                     DRSIDVIRTVARDLGMGRA"
     gene            2134..2838
                     /locus_tag="VFR25_03630"
     CDS             2134..2838
                     /locus_tag="VFR25_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET9940169.1"
                     /translation="MFMPFWDPTMLLLIPAMILAFYAQTKVKSTYQKYSEIMAASRRT
                     GRDIARTILSQNGITDVEVVEGEGFLSDHYDPIKKVVRLSPDNFSQPSVAAISVAAHE
                     VGHAIQHAKGYAPLQLRTAIFPLANIGTMLAWPILLIGLFFVPGVRIAGVSLIDIGIL
                     LFSFGVAFQVITLPVEFDASRRALVQLNQLGLVSPEEQDGAKKVLDAAALTYVAAAAA
                     AILQLVRLLILRDRRN"
     gene            complement(2932..3210)
                     /locus_tag="VFR25_03635"
     CDS             complement(2932..3210)
                     /locus_tag="VFR25_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9940170.1"
                     /translation="MTKADIVDEIAERTGLTKKDVADTVDQFLDAVSRALASGKHIEI
                     RGFGTFKVKDRKSRLARNPRTGESVPVPPRRVPVFKVSKELKDIVAQI"
     gene            3691..4629
                     /locus_tag="VFR25_03640"
     CDS             3691..4629
                     /locus_tag="VFR25_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9940171.1"
                     /translation="MKQVLLFFIDGLGVGSDDPDVNVVASGDYPTLALTRTHRPGSRS
                     GLIPVLAHGVDASLGVPGLPQSATGQTAILTGVNAPAVMGRHISGFPGPTLRAILAEH
                     SILKRIREAGRSASFLNAYGTTFFQTEPSKRRLSATSLATIASGAPFRTLEDLLAGRG
                     VVHDLTHWRMRERGYDVPLRTPEEAGAIIAEQAMAQDFSLFEYFETDRAGHDQDAERA
                     KRCLSDLDRALETVLALADLERLSVVVVSDHGNVEDGTVRTHTLNPAHFALWGSWTPK
                     QTPERLTDIAALCFEALGLPGGAPGGPAGKTATSAA"
     gene            complement(4633..5202)
                     /locus_tag="VFR25_03645"
     CDS             complement(4633..5202)
                     /locus_tag="VFR25_03645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9940172.1"
                     /translation="MTRNHAAWILIASLALAGCAGGKKSSDTAPDTTASTAPAAGSTT
                     AAPSGGTMPDVSTGAAPGTMPVTPNPTPAHITVQHILIGFAGSVPGKGITRSQDDAKK
                     LAYDILARARKGEDFDALVTSYTDDSPPGIYSMANTGVSKGMDEFQREQMVPAFGNVG
                     FAISVGNIDIADYNPTTSPYGWHIIKRLK"
     gene            complement(5237..5872)
                     /locus_tag="VFR25_03650"
     CDS             complement(5237..5872)
                     /locus_tag="VFR25_03650"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HET9940173.1"
                     /translation="MKRTTDPTLPAAETSAPGYPEGDRRRVPEGLTLVPTKIFFTKGV
                     GSHRDELRSFELALKDAGIERLNLVHVSSIFPPLCKIISREEGSKLIAPGSIAFCVMA
                     RQASNEMRRLIASSIGCALPADKNTYGYLSEHHAYGQTDEVAGDFAEDMAASMLASTL
                     GVEFDENANWDEKEQVFKISGEIVRTFNVTQSHVIANKGWTTVVAAGVFIF"
     gene            complement(5913..6617)
                     /locus_tag="VFR25_03655"
     CDS             complement(5913..6617)
                     /locus_tag="VFR25_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET9940174.1"
                     /translation="MWARIRDLLLHNLGLKLASLLLALLLYAHVVTDQQRESRLTIPL
                     ALTGLPDTLALSGRVPSRVVVDVRGKWKDLIRLGLIGRQLTVDLAGVSPGRFQKSISA
                     DDVRERAIPAELAKAVDVTNVIEPRTVDVVIEAKRRRSVPLLARLTGSPAEGYSVVGA
                     PEIDPDSAIVAGPQSLVQNLDTLRTLPVDIAGERERIQRQVQLDTGASPIVVQPRRVL
                     VTLRIARTAPDSMAHP"
     gene            complement(6619..7236)
                     /locus_tag="VFR25_03660"
     CDS             complement(6619..7236)
                     /locus_tag="VFR25_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940175.1"
                     /translation="MTSPTLRHVPWLRLAAMLAAAAIVVALHAARADERPASAGKAAP
                     AGPATGQIGIRLVRLLSEVPEDVEKRSGPLHDLRVELVPGNVIVFRKGSEFAALLPID
                     KIQGRVDSLRYFYYVERSPMFWFFGGARLKGIRAVADGSAFQFDTFRLFWGNGGEQAG
                     WIYFPDTAENQGLRFSVVSGQSVDQADPKETKYWVELGSEGKAGF"
     gene            7396..8268
                     /locus_tag="VFR25_03665"
     CDS             7396..8268
                     /locus_tag="VFR25_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HET9940176.1"
                     /translation="MRLKLPISGPLDLEATFSCGQAFRWRRDADGPWTGIVGGAELRV
                     LGVGDGVLTLETTGPSDAASLERYFRLDENPEGHVDGARELLAIPGFVPLLGLRLLRQ
                     DPWETLASFICSAAANIGKISANVEAVASRHGTDISGSERRAFPGPLAIARARESDLR
                     RAGVGFRAPYLLGTAKRVAEDPDAFWARLRSAPLEEARDELTSLPGVGPKIADCVLLF
                     GLDRLEAYPVDRWIRRATRELMARPQARDEELARWAVRLGPGRGYLQQVLFHLRRTSG
                     PFPSMAALRRRAAG"
BASE COUNT         1258 a         2872 c         2838 g         1342 t
ORIGIN      
        1 cagaggccgg agagcccgag cacgacggcg gcgctcagga agtcgccgcg cgcgaggagg
       61 agcgcggcga ggagggacag gccgagtccc gcgatggtca gatggtcggg cttgacgccg
      121 cgcttcgcga gcgcggcccc gatgggacgg aaccaggatc gcccgcgctc cttccagccc
      181 tccgtcattc cctctcctcc cattcgtccc gtggcggtgg ctcttccgcc tcctcgcggt
      241 cgtcgccgtc cttgggcgca ccgccgacca cgagcacgaa ctcgccgcgc ggcgtcttcg
      301 ctccgaccca cgcgaggagc gacgagaggg gaccgcggcg ggtctcctcg aatttcttcg
      361 tcaattcgcg cgagatcgaa gccggccggt cccccagctc cgccatgagg tcgcggagcg
      421 ccgcggcgac ccggtgcggc gattcgaaga agatgagggt gcgagggtcc tcgcgcaact
      481 cccggatgcg cgtgcgccgc cgcccgggct ttcgcgggag gaatccctcg aacgcgaatc
      541 ggtcggtggg aagtccggac acctcgagcg cgcagagaac gctcgacggg ccggggatcg
      601 gcacgacgcg gaggtcctcg cgcacggcgg cgcgcacgag cgtgaacgcg ggatcggaga
      661 tcccgggcga gcccgcatcg gaaaccacgg ccacggacat cccctcgcga aggcgcccca
      721 cgagctccgg ggtccgcgcg tccttgttgt ggtcgtgata cgagacgagc gcgcgatcga
      781 tgccgaagcg cgcgaggagt ccccgcgtgt gccgcgtgtc ctcggcggcg acgacgccgg
      841 cctcggcgag gatgtgccgc gcgcgcggcg agaggtcctc gacgttgccg atgggagtcg
      901 cgacgagata gagggtgccg ggctcgggct cgccgctcac gcgcgaccca tccccagatc
      961 gcgggccacc gtccgaatca cgtcgatcga gcggtcgatg gcggggtcgt ccacgtcgag
     1021 gtgcgtcgcc gcgcggaatc ggccggggcc gccgaagacg aggagcctca ctcccgcttc
     1081 cgcggcgcga tccgccagct cctgaacgcg ttcgtgcggc gtcacctcga cgatgacgat
     1141 gttcgtggtc acggtcttgg gatcgatggt gaggcccggc acggaggcga gcgcctcggc
     1201 gagccgccgc gcgcgcccgt ggtcgtcgac gaggcgctcg cgatgatggc gaagcccatg
     1261 gagcgcgccc gcagcgagga tccccgcctg ccgcatgccg ccgccgaagc gcttccggag
     1321 cgtgcgcgct tcaccgatca gagccttcgg cccggcgatc gccgacccca ccggcgcacc
     1381 gagccctttc gaataacaaa ccatgaccgt gtccgcggcg tcggcgaact cggagaccgg
     1441 cgtgcccgtt gcggccgagg cattccagag gcgcgcaccg tccaggtgca cggggagacc
     1501 gcgctcgcgg gcgggcctgg ccgcgcggcc gagccgctcg acgtcgtaga ccgcaccgcc
     1561 cgcccagttg tggctgttct cgagctccac caatcccacg cgtccgttcc cttcgcgcgc
     1621 ggccgagtcg agcgccgccg cgatcgattc cggcgcgagc cgtcccgcgt cgccgacgac
     1681 gggcaggagt cgaagtccga ggaagctcgt gagcgcggct tcctcggagt aagcgacgtg
     1741 gcttcgctcg tgcaggagca cctggtcccc gcgccgagtc tggatgccga tcgcgatctg
     1801 gttccccatg gagccgctcg gcacgaagag agccgactcc ttgccgagga gccgggccgt
     1861 ctcttcctcg aggaggcgca ccgtcgggtc ctcgccgaag tgatcgtccc cgacgtcggc
     1921 ttcggccatg gctcggcgca tcgccgcggt cggccgggtc accgtgtcgc ttctcagatc
     1981 gacgtaagcc gttgtcaatc aagccccgat tcgttgaatc ggcccagggc cgatgatagt
     2041 ctcagtgtcc agaaggggtg gaaccgttcc cgcagcacgg cagtataggg gagcggactc
     2101 acgcccccca actcgaacgg aggtaaccgc acgatgttca tgccgttctg ggatccgacg
     2161 atgctgcttc tgatccccgc gatgatcctg gccttctacg cccagaccaa ggtcaagtcg
     2221 acgtaccaaa agtacagcga gatcatggcc gcgagccggc gcacgggacg cgacatcgcg
     2281 cgaacgattc tgagccagaa cgggatcacg gacgtcgagg tggtcgaagg cgagggcttc
     2341 ctctcggatc actacgatcc gatcaagaag gtggtccgcc tgtcgccgga caacttctcg
     2401 cagccctcgg tggccgcgat cagcgtggcc gcgcacgagg tggggcacgc catccagcac
     2461 gccaagggct atgcgccgct ccagttgcgg accgcgatct tccctctcgc caacatcggt
     2521 acgatgctgg cgtggcccat tctcctgatc gggctcttct tcgtgccggg tgtccggatc
     2581 gcgggcgtga gcctgatcga catcggcatc ctcctcttca gcttcggggt ggcgttccag
     2641 gtcatcaccc tccccgtgga gttcgacgcg agccgccgcg ccctcgtcca gctcaaccag
     2701 ctcggtctcg tctccccgga ggagcaggac ggtgccaaga aggtgctgga cgccgccgcg
     2761 ctgacgtacg tggccgccgc ggccgcagcc atactccagc tcgtccggct cctgatcctt
     2821 cgagaccggc gcaactgagc gcgcttccac acgagacgag aaaacgggac gtcccgacac
     2881 cggggcgtcc cgtttcgatt tcaatcgaca ggccctctcg ggccgaatca gctagatctg
     2941 ggcgacgatg tccttgagct ccttggacac cttgaacacg ggtacgcgtc gcggcggaac
     3001 cggcaccgac tcgcccgtgc gcggattgcg cgcgaggcgc gactttcgat ccttcacctt
     3061 gaaggtcccg aagccccgga tctcgatgtg cttacccgag gcgagggcgc gcgacaccgc
     3121 atcgagaaac tggtcgacgg tatccgccac gtccttcttg gtcaggccgg tccgctcggc
     3181 aatttcgtcg acgatatcag ccttggtcat cagaccctcc tggcgagccc actccgcccg
     3241 cgcttccggt gcgcggggca cggtgcccag ctccgggcgc actctccgcg ccgcgggtcg
     3301 ctataaagtg gagcgggtat tacgattaag cgaaccgcgg cacgcgaaac gggactatag
     3361 acctgccgcg aagcgtgtca aggctttttc ccgctacggt ttccgggttc aaagcgccgc
     3421 ctgcaacgcc cgcggcggct tctcgggcat ttggaagggc cgatacgtag tcgccgaaaa
     3481 tccgggggcg agcagtcccc caatcgattg gccagggcga ggttacggaa gcatggaatc
     3541 ctgccccatg gcacgtgcgt tgcgtaagcc gatcccagac ttcgaactcg ggagccccct
     3601 agcgcaggtc cttgaatcca cgtcgccggc cctgcaccga cccagcgcta gctgtgcggc
     3661 tcccgttttc gtttcaggcg gtcccacccc atgaagcagg tccttctctt cttcatcgac
     3721 ggcctcggcg tcggctccga cgaccccgac gtgaacgtgg tcgcgagcgg cgactacccc
     3781 acgctcgcgc tcacaaggac gcatcgcccc ggatcccgtt ccggcttgat ccccgtcctg
     3841 gcgcacggcg tggacgcctc gctgggtgtc ccgggacttc cccagagcgc gaccgggcag
     3901 accgcgatcc tcaccggggt gaacgcgccg gccgtgatgg gacggcacat ctccgggttc
     3961 cccggcccga cgctccgggc gatcctcgcg gagcactcga tcttgaagcg gattcgcgag
     4021 gcgggccgat cggcgagctt cctgaacgcg tacggaacga ccttcttcca gacggagccg
     4081 tccaagcgcc ggctctcggc gacatcgctc gccacgatcg catcgggcgc gccgttccga
     4141 acgctggagg atctcctcgc ggggcggggc gtcgtgcacg acctcaccca ctggcgcatg
     4201 agggagcggg gttacgacgt tccgctcagg acgccggaag aggcgggagc catcatcgcg
     4261 gagcaggcga tggcccagga cttctcactg ttcgagtact tcgagaccga tcgcgcgggg
     4321 cacgatcagg acgcggagcg cgccaagcgg tgtctctcgg atctggatcg cgcgctcgag
     4381 acggtgctcg cgctcgcgga cctcgagcgt ctctcggtcg tcgtggtgag cgatcatgga
     4441 aacgtggagg atgggacggt gcggacccac acgctgaatc ccgctcactt cgcgctgtgg
     4501 ggatcgtgga cgccgaagca gacgcccgag cggctgaccg atatcgccgc tctatgcttc
     4561 gaagccctgg gcctgcccgg cggtgcgccg ggcgggccag cagggaaaac cgctacttca
     4621 gccgcttgac cgctacttca gccgcttgat gatgtgccag ccgtaggggc tggtcgtcgg
     4681 attgtagtcc gcgatgtcga tgttcccgac gctgatcgcg aagccgacgt tcccgaacgc
     4741 gggcaccatc tgctcgcgct ggaactcgtc cattcccttc gacacgcccg tgttcgccat
     4801 gctgtagatc cccggaggcg agtcgtccgt gtacgacgtg acgagggcgt cgaagtcctc
     4861 tcccttccgc gcccgcgcca ggatgtcgta ggcgagtttc ttcgcatcgt cctgcgatcg
     4921 cgtgatgccc ttgcctggca ccgagcccgc gaatccgatg aggatgtgct ggacggtgat
     4981 gtgcgccggg gtcggattcg gcgtgaccgg catggttccg ggggcggccc cggtcgaaac
     5041 atcgggcatg gtgcctccgg aaggcgcggc cgtcgtggat ccggcggccg gcgcggtcga
     5101 tgcggtggtg tcgggagcgg tatcgctgga cttcttgccg cccgcacagc ccgcgagggc
     5161 gagcgaggcg atcaggatcc aggcggcgtg attccgggtc atgggactcc tcatcgatgg
     5221 gtgatggggt tggggatcag aagatgaaca cgccggcggc gacgacggtc gtccatccct
     5281 tgttcgcgat gacgtgcgac tgcgtgacgt tgaacgtgcg gacgatctcg ccggagatct
     5341 tgaacacctg ctccttctcg tcccagttcg cgttctcgtc gaactcgacg ccgagcgtgg
     5401 aggcgagcat ggacgcggcc atgtcctccg cgaagtcgcc ggccacctcg tcggtctggc
     5461 catacgcgtg gtgctcgctc aggtatccgt aggtgttctt gtcggccggc agcgcgcagc
     5521 cgatggagga ggcgatgagc cggcgcatct cgttcgacgc ctggcgggcc atcacgcaga
     5581 acgcgatcga gccgggggcg atgagcttgc tcccctcctc gcgcgagatg atcttgcaca
     5641 gcggcgggaa gatgctcgac acgtggacca ggttgagccg ctcgatgccg gcatccttga
     5701 gcgccagctc gaaggagcgc agctcgtccc ggtggcttcc cacacctttg gtgaagaaga
     5761 tcttggtggg aaccagcgtg agcccttcgg ggactcgccg ccggtcgcct tcggggtagc
     5821 ccggtgccga agtctcggcg gcaggcagtg tcgggtcggt ggttctcttc atctcgtcgc
     5881 gatctccttt tccgggaatt gcaccgtcgg gatcaggggt gcgccatgga gtcgggcgcc
     5941 gtccgggcga tgcgcagcgt cacgaggaca cgccgcggct ggaccacgat cgggctcgcg
     6001 cccgtgtcca gctggacttg acgctgaatt ctctctcgtt ctccggcgat gtcaaccggt
     6061 agcgtgcgca gggtgtcgag attctgcacc aaagactgcg gtccggcgac gatcgccgag
     6121 tcggggtcga tctcgggggc gcccaccacg gagtagccct cggccggcga tccggtcagg
     6181 cgcgccagga ggggcaccga ccgcctccgc ttcgcctcga tgaccacgtc caccgtccga
     6241 ggctcgatga cgttggtcac gtcgaccgcc ttggccagct ccgccgggat ggcccgctcg
     6301 cgcacgtcgt ccgccgagat cgacttctgg aagcgcccgg gcgatacccc ggccagatcc
     6361 acggtgagct gccggccgat caggccgagc cggatcagat ccttccactt gcctcgcacg
     6421 tccacgacca cccggctcgg cacgcgtccc gacaaggcca gggtgtcggg aaggccggtc
     6481 aaggcgagcg gaatggtgag cctggactcg cgctgctggt cggtgacgac gtgggcgtag
     6541 aggaggagcg ccaggaggag tgacgcaagc ttgaggccga ggttgtggag gaggaggtcg
     6601 cggatccggg cccacacgct agaagcccgc cttcccctcg gatcccagct ccacccagta
     6661 cttcgtctcc ttcggatcgg cctggtccac cgactgtccg ctcaccacgg agaagcgaag
     6721 tccctgattc tcggccgtgt ccggaaagta gatccagccg gcctgctcgc ccccgttgcc
     6781 ccagaacagt cggaacgtat cgaactggaa cgcgctcccg tccgcgacgg cgcggatccc
     6841 cttcaagcgc gcgccgccga agaaccagaa catcgggctc ctctcgacgt agtagaagta
     6901 gcggagcgaa tcgacgcgtc cctggatctt gtcgatggga aggagcgcgg cgaactcgga
     6961 acccttgcgg aagacgatca cgttgcccgg cacgagctcc acgcggagat cgtgcaacgg
     7021 cccgcttcgc ttctcgacgt cctcgggcac ctcggagagg aggcgcacca gacggatgcc
     7081 gatctgaccc gtggccggcc cggcgggcgc ggccttcccg gccgacgccg gtcgctcgtc
     7141 cgcgcgcgcg gcgtggagcg cgacgacgat cgccgccgcc gcgagcatcg ccgcgaggcg
     7201 caaccatggg acatgccgga gcgtcggtga ggtcatcgtt ggttgtgccg ggatcgggcg
     7261 tccgcgttcg tggggtagat gagtcaagcg ccgggcgatc ttcccacgcc aaatcgtgat
     7321 gcgtcaacgg aaacccccgt tcgcgccctc ggaatggcat tgacggcgcg ccgccgcgcg
     7381 gacgaccctc gtcccgtgcg cctcaagctg cccatctcgg gccctctcga tctcgaagcc
     7441 acgttttcgt gcggccaggc cttccgctgg cgccgagatg cggacggccc ctggaccggg
     7501 atcgtcgggg gcgccgagct gcgggtgctc ggcgtcgggg acggcgtcct cacgctcgag
     7561 accacgggtc cctccgacgc agcctccctc gagcgctact tccgtctgga cgagaatccg
     7621 gaggggcacg tggacggggc ccgcgagctc ctcgcgatcc cgggcttcgt gccgctcctc
     7681 gggctccggc tcctccgcca ggatccgtgg gaaaccctcg cgtcgttcat ctgctccgcc
     7741 gcggcgaaca tcgggaagat cagcgccaat gtcgaggcgg tcgcgtcccg gcacgggacg
     7801 gacatttccg gctccgagcg ccgcgcgttc cccggtccgc tcgccatcgc gcgagcgcgc
     7861 gaatcggacc taagacgcgc gggcgtcggc tttcgcgctc cctacctgct cggcaccgcg
     7921 aagcgcgtgg cggaggatcc ggacgcgttc tgggcccggc tccgctcggc tccgctcgag
     7981 gaggcgcggg acgagctgac ctcgcttccc ggcgtgggcc cgaagatcgc cgactgcgtg
     8041 ctcctcttcg gtctcgaccg gctcgaggcc taccccgtcg accggtggat ccgccgcgcg
     8101 acgcgcgagc tcatggcgcg cccccaggcg cgcgacgagg agctggcgcg ctgggccgtg
     8161 cggctcgggc ccggacgcgg ctacctccag caggtcctct ttcatctacg gcgaacgtcg
     8221 gggccgttcc cctcgatggc ggcgctccgc cggcgcgcgg ccggatgaag gcggcggtcg
     8281 cgatcctcgc tcgagccccc gtgccgggcc
//
LOCUS       DASTKC010000085         5341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1956623, whole genome shotgun sequence.
ACCESSION   DASTKC010000085 DASTKC010000000
VERSION     DASTKC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956623"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..715
                     /locus_tag="VFR25_03670"
     CDS             245..715
                     /locus_tag="VFR25_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940177.1"
                     /translation="MDFVLREDDLRGPEVAELLQAHLDFAALQSPRESIHALDLDRLR
                     VPDITFYTVWLGRDLAGCGALRELAADHGEIKSMHTAARHRGKGVGARLVAHMLDVAR
                     ARGYRRVSLETGTADAFAPARALYGRFGFTVCPAFAQYRDDDPHSLCMTLALGA"
     gene            complement(720..1571)
                     /locus_tag="VFR25_03675"
     CDS             complement(720..1571)
                     /locus_tag="VFR25_03675"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9940178.1"
                     /translation="MLTGLQIDIAALTDRGKRREANEDAYSVFRVGRFAERVDSSVPE
                     SELPSFYDARALAIGVADGMGGHAAGEVASRDALVELMRLALKKPRWMMYLDDPETRE
                     GEIQDFFDRTKTYLAGVHAAILDEGKEDPGKRGMGTTFTLAYLVGLDLFIVHVGDSRV
                     YRLHQGALTQLTRDHTVAQRLADAGVIPQEVVATHPERHRLTQVVGGSGGWIRGETHH
                     LRLEAGDALLVCSDGLNAVVPDEEIARCIAGARSSQDACRDLVSRTLSGGAPDNVTVV
                     VARLGSG"
     gene            complement(2200..2727)
                     /locus_tag="VFR25_03680"
     CDS             complement(2200..2727)
                     /locus_tag="VFR25_03680"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9940179.1"
                     /translation="MRVTTRALMPLSVCVAIAWFAAAGADQPTPRPASQITEGRTLIT
                     LPDTTGYSPDERAILTLERGRSAAIARGDTAWLATLYATDFRGVAGNGARIERTDLFR
                     IFGRDDPVSRFLIDELSIHSYGNAATVTGRLRTLGPTGDMTAESRYLHVYAMRESHWR
                     LVAAQATVVPQPAKR"
     gene            complement(2746..3528)
                     /locus_tag="VFR25_03685"
     CDS             complement(2746..3528)
                     /locus_tag="VFR25_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HET9940180.1"
                     /translation="MESHPVILTILIPLLMLAGMWACLEIGFRVGRKRRASRTGETND
                     EITAVVAAVLGLLGLVLAFAFGNAQERLTVRRAQIVDEANAIGTAYLRLDLLAPEDQG
                     PLRPLFREYLETRIATFERITDRAAYKVLLDRGARLQEEIWRRSVAASRASPNQGTPI
                     LLLDALNRMIDITTTRDLATTTHAPFLILALLVGLSFMGALLAGDTLALKGRRYPLHM
                     ALFALAIAATVYIVIDLEYPRVGFITLRHMDRAMIQLRDLMH"
     gene            complement(3626..4915)
                     /locus_tag="VFR25_03690"
     CDS             complement(3626..4915)
                     /locus_tag="VFR25_03690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9940181.1"
                     /translation="MGALQRASSAIALITLTLFGVCLAVTFPKAPRPPEPRRVPELWA
                     TGDPADTLSRGAILSHVLATKGLDERQIREITRLLRTYKSPNSLRPGASVRFYPRGGG
                     VPNKIRLDITPDSTLRFVRVLSSWTAHVELKPFTMDTVVVSGLIETSLWASHLGGDVD
                     RLGKRGFEDLVYDMADVFAWKVDFTRDLRKGDSFRLALERKIRGDGSIRSRHFLAIEL
                     TNGGRVLRAIPRPRANGRFAYYDEDGHSLHGAFLRYPVPYRITSRFTSRRYHPILKRY
                     RAHEGIDYGAPTGTPVDVTASGVVTRAGWAGGYGRLVEVRHAQGIRTRYAHLSSIAVG
                     IRPGAKVDQGDVIGRVGSSGLATGPHLHYEFVKDGHHQNPLTVEMPGQAPLASSEMDG
                     FKKDRDHALALLEGVSIPEETRFATAQHDTGRRASRR"
     gene            5025..>5341
                     /locus_tag="VFR25_03695"
     CDS             5025..>5341
                     /locus_tag="VFR25_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET9940182.1"
                     /translation="MRRATLILSPLLALSMMTALPSGAAPSDAPVRPPKPFDASVYAA
                     RREKLMKTLGKGIAVLYARGEEDRDGYRQDSDFYYLTGISEPGAVLVLSPAERTYHER
                     LYL"
BASE COUNT          860 a         1743 c         1859 g          879 t
ORIGIN      
        1 gctgtcatcg ccgcatccgg tggccaggat ggtcgcgagc aggcccaggc atagcaggtg
       61 gaacgagggg gcgggcttcg gggcgacgag gtccgggcgg atgcggagca gggcagtcat
      121 gtgcacctct tggctggatc cacggtggag cccgtggatc cgacagagcc agcaacctag
      181 tatatcaaac cggaactgag gaggcattcc gtccgcccgc gcgatccgct aggattcgcg
      241 ctccatggat ttcgtccttc gtgaagacga tctccgcggt cccgaggtcg cggagctgct
      301 ccaggctcac ctcgacttcg ctgcgttgca atccccgcgc gaaagcatcc acgcgctcga
      361 cctcgaccga cttcgcgtgc cggacatcac gttctacacg gtctggctgg ggagagactt
      421 ggcgggctgc ggggcgctcc gggagctggc ggcggatcac ggagagatca agtcgatgca
      481 cacggcggcg aggcatcggg ggaagggtgt gggggcacgg ctcgtggcgc acatgctcga
      541 cgtcgcgagg gcccggggct atcgacgcgt gagcctcgag acgggcaccg cggacgcgtt
      601 cgctcctgcc cgcgcgctct acggccgatt tgggttcacg gtctgtcccg cattcgcgca
      661 ataccgggac gacgacccgc acagcctctg tatgaccctc gcgctcggcg cctgactttc
      721 tacccggacc cgaggcgagc cacgacgacg gtgacgttgt ccggggcgcc accggaaagc
      781 gttcgactga cgaggtcccg gcaagcgtcc tggctcgatc gggcgcccgc gatgcatcgg
      841 gcgatctcct cgtcgggaac caccgcgttc agtccgtcgg agcacacgag gagcgcgtct
      901 ccagcctcga gccgcaggtg atgcgtctcg ccgcggatcc atcctccgga tccaccgacg
      961 acctgcgtga gtcgatgccg ctcaggatgc gtggcgacga cctcctgcgg gatcaccccg
     1021 gcgtccgcga ggcgctgcgc caccgtgtgg tcgcgggtca gctgggtgag tgctccttgg
     1081 tgcaggcggt agacccgcga gtcgcccacg tggacgatga agagatcgag tccgaccagg
     1141 taggcgaggg tgaacgtggt gcccatccct cgcttgcccg gatcttcttt tccctcgtcg
     1201 agaatcgccg cgtgcacgcc ggccaggtag gtcttggtgc ggtcgaagaa gtcctggatc
     1261 tcgccctcgc gcgtttcggg gtcgtcgagg tacatcatcc agcgcggctt cttgagcgcc
     1321 aagcgcatga gctcgactaa ggcgtcgcgg ctcgcgacct cgccggccgc gtggccgccc
     1381 attccgtcgg cgactccgat ggccagcgcg cgggcgtcgt agaaggaggg aagctccgac
     1441 tcgggcacgc tgctgtcgac gcgctcggcg aagcggccga cccggaagac gctgtacgcg
     1501 tcctcgtttg cttcgcgtcg ctttccgcga tcggtcaggg ccgcgatgtc gatctggagg
     1561 ccggttagca tggccgccgg agtatagcct ccatccatag ggcgtcctag cccggtcgtc
     1621 gcgcgcggag ccggtcggcc atggcgcaag cgatgcgccc cggcggagtc gcccccgccg
     1681 gggccgttct actcatcgtc ctcgtcgtcg agcgatacgg ccttgtccgg atcgccggtc
     1741 atgagcagcc ggcatacctg gcacaggatg gccggttccg acgacagcag atagtggtcc
     1801 ggaacgaccg gcgcgccgca gtcatagcag gctcgaaccg ggttgaaatc ggctcttccg
     1861 gtacgcctgt cgcagctgtt tccggccccg gatccgctcg agcgtccgcg ggtccagctc
     1921 gtggctcttc ttgcggcgga gccgcgccca cgcgcgcgtc cacctcgacg tccacttctt
     1981 gatttgcgtt tgcacgggaa tctcctttcg tgccgggacg cgcgtcccgg ccgatcggcg
     2041 gattcccgcg agacggctta gggggcgagc gtgacccgga tggtcggcgt gacgctgggt
     2101 cctgcgatca aggcacgcat gcgctcctcc gggttggtag gtggtgaacc gtggtgactt
     2161 cgaggctcga tggtagcgcg ggaccgcccc gcgccacaac tatcgcttgg cgggctgcgg
     2221 caccaccgtg gcctgggccg ccacgagccg ccagtggctc tcacgcatgg cgtagacgtg
     2281 caggtatcgc gactccgcgg tcatgtcccc ggtcggcccg agcgtccgaa gacggcccgt
     2341 tactgtggcg gcgttgccgt aggagtggat cgagagctcg tcgatgagaa agcggctgac
     2401 gggatcgtcg cgaccgaaga tccgaaagag atcggtccgc tcgatccggg cgccgtttcc
     2461 cgcgactcca cgaaaatcgg tggcgtacag ggttgccagc caggccgtgt cgccgcgcgc
     2521 gatggccgcg ctccggccac gttcgagcgt caggatcgcc cgctcgtccg gcgagtagcc
     2581 ggtggtgtcg ggaagcgtga tgagggtcct gccctcggtg atctggctgg ccggacgcgg
     2641 cgttggctgg tccgcgcccg ccgccgcaaa ccacgcgatg gcaacgcaca cggacaaggg
     2701 catcagggca cgcgtcgtga ctctcatcgc catctccaga gccggctagt gcatcaggtc
     2761 ccgaagctga atcatcgcac gatccatgtg ccgcagggtg atgaacccca cgcgcggata
     2821 ctccaggtcg atcacgatat agacggtcgc cgcgatggcc agggcgaaga gcgccatgtg
     2881 gagcggatac ctccggccct tgagcgcgag ggtgtctccc gcgaggagcg cccccatgaa
     2941 cgaaagcccg acgagcagtg cgagaatgag gaagggtgcg tgcgtcgtgg tggccagatc
     3001 gcgcgtggtc gtgatgtcga tcatcctgtt gagcgcatcc aggaggagga tcggtgtccc
     3061 ctgattcggc gacgcgcggg acgcggcgac acttcgcctc cagatctcct cttgaagtcg
     3121 agcgcctcga tccagcagaa ccttgtacgc cgcgcgatcg gtgatccgct cgaaggtggc
     3181 gattctcgtc tctaggtatt cccggaagag cgggcgaagt ggcccctggt cctcgggggc
     3241 cagcaaatcg aggcgcaggt aggctgtgcc gatcgcgttg gcctcgtcca cgatctgtgc
     3301 ccgtcgtacg gtgagacgct cctgggcgtt gccgaacgcg aacgccagca cgagcccaag
     3361 gaggcccaga acggccgcga ccacggccgt gatctcatcg ttggtctcgc cggtccggga
     3421 cgcgcgtcgc ttgcgcccga cccgaaaccc gatctcgaga caagcccaca tcccagccag
     3481 catgagaagg ggaatgagaa tggtcagaat gacgggatgg ctttccatgt cgtgtcgaag
     3541 tcccgggacg tagcgacggg gcgagcgcgc gcgaacgtac gcgacgggtc agaggggagt
     3601 caagacgaac gagggtgaag cgtgactagc gccgcgaagc tctccgtccc gtgtcgtgct
     3661 gcgcggtggc gaagcgagtt tcctcgggga tggacacgcc ctcgaggagc gcgagggcat
     3721 gatcgcggtc cttcttgaag ccgtccatct cggacgaggc cagcggagcc tgccccggca
     3781 tctcgacggt gagtggattc tggtgatgcc cgtccttcac gaactcgtaa tggagatgcg
     3841 gccccgtcgc gagccccgag gatccgacgc gcccgatcac gtccccctgg tccaccttgg
     3901 cgccgggacg gatgcccacc gcgatcgagc tcaagtgcgc gtaccgcgtg cgaattccct
     3961 gcgcgtgccg aacctcgacg agccggccgt agccgccggc ccatcccgcg cgcgtcacga
     4021 cgccggacgc ggtcacgtcg acgggcgtgc cggtgggcgc cccgtagtcg attccctcgt
     4081 gggctcgata ccgcttcaag atcggatggt agcgccggct cgtgaagcgg ctcgtgatcc
     4141 ggtagggaac gggatagcgc aggaacgcgc cgtggagcga gtgcccgtcc tcgtcgtagt
     4201 acgcgaagcg gccgttcgcg cgaggacgcg ggatcgcgcg cagcacgcgc ccgccgttgg
     4261 tcagctcgat cgcgagaaag tggcggctcc ggatcgagcc gtcgccgcga atcttccgct
     4321 cgagcgcgag acggaacgaa tcccccttcc ggaggtcgcg cgtgaagtcc accttccacg
     4381 cgaacacgtc ggccatgtcg tagacgagat cctcgaatcc gcgcttgccg aggcgatcga
     4441 cgtcgccgcc caggtgcgag gcccagagac tcgtctcgat gaggcccgac acgaccacgg
     4501 tatccatggt gaagggcttc agctcgacgt gcgcggtcca ggacgaaagg acgcgcacga
     4561 atcggagcgt ggagtccggc gtgatgtcga ggcggatctt gttcggcacg cctccgccgc
     4621 gcggatagaa ccggacggag gctccggggc gaagcgagtt cgggctcttg taggtgcgca
     4681 ggagccgcgt tatttcccgg atttgccttt cgtcgagacc cttcgtcgcg aggacgtggg
     4741 agaggatcgc gccacgcgac agcgtatcgg ccggatcgcc cgtcgcccag agctcgggga
     4801 cgcggcgcgg ctcggggggc cgtggggcct tggggaaggt gacggcgagg cacacaccga
     4861 acaaggtcag cgtgatcaga gcgatggcgg aagaggctcg ctggagggcg cccatcggcc
     4921 attcatcggc cggtcgcctc cggttcctaa ggagatgttg ccgagggcct tagcggctat
     4981 actgccgccg cttcgggccg atccaccgat tcggaggact cgatatgcga cgcgccacgc
     5041 tgatcctttc gccccttctc gcgctctcga tgatgacggc cctcccaagc ggagccgcac
     5101 cgtccgatgc tcccgtgcgt ccccccaagc ccttcgacgc ctccgtctac gcggcgcggc
     5161 gcgagaagct catgaagacg ctcgggaagg ggatcgcggt gctctacgcg cggggcgagg
     5221 aggatcgcga cggctacagg caggacagcg acttctacta tctcaccgga atatcggagc
     5281 ccggcgcggt gctcgtcctc tcaccggcgg agcggaccta ccacgagcgt ctctacctca
     5341 a
//
LOCUS       DASTKC010000086         8427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1720250, whole genome shotgun sequence.
ACCESSION   DASTKC010000086 DASTKC010000000
VERSION     DASTKC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720250"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFR25_03700"
     CDS             <1..276
                     /locus_tag="VFR25_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940183.1"
                     /translation="HPGIHVSLVSPAVVATEFGLHALGGGPDSRQLPQAQSVEEVARV
                     IVDVIERPRADVYTRPELQERVVEYFAAEDLAEVEARTALVIRPSRA"
     gene            complement(326..1552)
                     /locus_tag="VFR25_03705"
     CDS             complement(326..1552)
                     /locus_tag="VFR25_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9940184.1"
                     /translation="MAIDVREGVGIAFLALRENKLRSFLTVLGVIIGITAIMAMISII
                     EGLNQSMKAQLASLGTDVLYIRPFAPGAWVGEMPDSLRRRKWFTPEDADAIRRTAPAV
                     LAVAPLNFTELRLRNGETQTRPTIVIGTSPDYMVTNNYAVDNGRAFTEAEVEHRSAVC
                     ILGVDHVETLFPHVNPVGRTIAIGTQPFMVVGVAEPRGHFLGMSMDELVLVPHTTLEK
                     DFGPKLKMVLNAKPVSPEMIDVAREQISDVLRRQRHVRYDQGDNFAVFTDKSLVDLYG
                     QITGAFYLVMVVISSIGLMVGGIGVMNIMLVSVTERTREIGVRKALGARERDILWQFL
                     VEAMTLTGAGGVIGVLAGLCVGKLVNLLAHFSFGVPIWGILLAFGSATAIGLFFGMYP
                     AARAARLDPVESLRYE"
     gene            complement(1556..2809)
                     /locus_tag="VFR25_03710"
     CDS             complement(1556..2809)
                     /locus_tag="VFR25_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9940185.1"
                     /translation="MTPIGRPRREAWAALAENLKIAVRALRAHKLRTVLTVIGNVVAV
                     MSVIAVVSIIDGVNKYVSERILDQGLNVVYIDKFGLITDDEAYREAAKRRDLTSYEAE
                     VLASRMQLASHVVAQAEGNKAVRYRGTELKNVRILGTTEGYPAVGKYETDQGRELEDM
                     DFQRRRPVCVIGSEVAETLFPRIDPIGQSIKVSGYELLVVGTLVARGNVLGQSQDNLV
                     VIPLGQFEKMYGSHRSLTIIVGAASGVTLDRLEDEARVVLRAARHVPLGAPDDFAFST
                     AETYMALYQTLTGGIFAGTIGLVAISLVVGGIVIMNIMLVAVTERTREIGIRKAVGAK
                     RSDLVSQFLSEAVVLAFFGGLLGVLAGVAIALLIRTITPLPATIQPWSVAISLTVASA
                     VGLFFGVYPATRAARLDPIVALRQE"
     gene            complement(2806..3471)
                     /locus_tag="VFR25_03715"
     CDS             complement(2806..3471)
                     /locus_tag="VFR25_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940186.1"
                     /translation="MGTTEVNALDGVSLRIMTGEYVAIMGPSGSGKSTLMNIVGCLDT
                     PTSGSYKLRGTEIRERDDDELARIRNQEIGFVFQTFNLLPRADALHNVELPLIYSGAP
                     ATERRERAKAMLAMVGLGDRTHHRPNELSGGQRQRVAIARALVNNPSLVLADEPTGNL
                     DSRTGIEILTLMDEIHAKGNTVVLVTHEEDVAARAHRIVRLRDGKIESDRPVARAGAT
                     PGA"
     gene            complement(3522..4820)
                     /locus_tag="VFR25_03720"
     CDS             complement(3522..4820)
                     /locus_tag="VFR25_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9940187.1"
                     /translation="MKGRTRIILIVAGVVLLLVIVLANLNRSAGGKIAVQAGDVRKGT
                     VTATVRAPGRVQPETQVKLSANVPGEVRTLPIHEGDSVRKGQFLLQIDDAQYRAQVGE
                     ANAALAAARSNLRLAEASLAQSQSVLKRKEALSTQNLVSAEELDQARTAASTDQSRVE
                     AGREDVRRLQSTLQAAHDNLRKTRFISPINGTVTQLTIERGEIAVIGTMNNPGTVLLT
                     VADLSRMKVEADVDETDVATIRIGQEATVKVDALPDTSLVGHVTQIANSPLISGADTQ
                     EQETNFKVDVTIDNPPPILRPGMTADVEIRTARRDSVLAVPIQSVVIRTPEDLKPPVT
                     KKTKKRGGAQAETAEKATPSGERPKQEEIRGVFVIVDGKAEFRRVKTGIASDTDFEVL
                     GEIKPGEKVVIGPHKTLRSLKPGQRVKIEEPKKAKGKESQ"
     gene            complement(4848..6344)
                     /locus_tag="VFR25_03725"
     CDS             complement(4848..6344)
                     /locus_tag="VFR25_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9940188.1"
                     /translation="MNLKRSWTPMLVLAAVLLRPATPAAQPPTAAPQPAVGDTVRFTP
                     ATPVESLLVAPITIPGERWSLAQAIQTALHSNADVRNAEARRKQASGAALSAWSGIIP
                     SLTTGASYNYFIPDKTSSFVGVTIPTPTSANPDSNVFATGLATDIRTGAIRADVTSNI
                     LSGPAIGAKKRLDHLRKGSEHDEQETRNNVVFQVKQQYFNLLKAIRLAQVAAQTEQLA
                     RDEEKRSEALLQVGTVARGDLLKARARRANTQVDHIRADNQVLIQSSRLAQVVGVDPT
                     LRIVPDAVLDDQVVLPDSSQAIREAVGTRPLMESARAVERAAHSGVFGAQTQRLPVVT
                     GSFGVSRAKTDQTIEDIDVAIPGAPSSVDTQHYATEWQGQVRLSLPIFTGFQTEGDVR
                     SAKGTLLEAESQRRQRELDVAAQVQEAWLNLREAVQRIDVEREGLASAEEDYKFSKGR
                     YDLGAGTYLDLLTAEVNLANARERLIEAVADARIAEANLEFATGEKRY"
     gene            complement(6439..7134)
                     /locus_tag="VFR25_03730"
     CDS             complement(6439..7134)
                     /locus_tag="VFR25_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9940189.1"
                     /translation="MTEVATAASELPEPSPLGAVAGVFVRPRETFESMRPHPRYWLPL
                     ILVVVAQVAFSMLVLQSGAVANDAVAKQEAKGAPPEQLEKMRAFFESPAAPVIIGVGG
                     LVTMAFIILLGSGVAFFMGNLMMGGGITYRHYLSAQAHGAAIGLLDQAIRTGIAWSKG
                     TLDIRLGLGNLLGDDIGPFGRMLDAATDPFILWSTAVVAIGISVYARKKFSFGLLAVL
                     PMFAVGLILSALR"
     gene            complement(7623..8348)
                     /locus_tag="VFR25_03735"
     CDS             complement(7623..8348)
                     /locus_tag="VFR25_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9940190.1"
                     /translation="MNAMAVPPSGWNPFGSREAVPARGSSRRNVKQGLALSALVHLSA
                     LGCILWIQAQHPEDALVLTRGRVDLVPPRVETPPLPRVGTTPPVRTDGGSIDPKLIMD
                     DPKTEPAVVDPKVGIAPGGGETGNQPSIPGDDERAMAQAVVDPDPSEETFVAFDEPPV
                     PIYNPDPAYPEWARENGIDGRVVLHALVGVDGRVRKVTVIRAIPGLSESAEETLYRWT
                     FKPARAGRQPVAVWIEVPFSFRL"
BASE COUNT         1304 a         2871 c         2842 g         1410 t
ORIGIN      
        1 cacccgggga tccacgtctc cctggtgtcg cccgcggtcg tcgcgacgga attcgggctt
       61 cacgccctcg gcgggggtcc ggattcgcgc cagttgccgc aggcgcagtc ggtggaggag
      121 gtcgcgcggg tgatcgtcga cgtgatcgag cgtccgcgcg ccgacgtcta cacgcgcccg
      181 gagcttcaag agcgggtcgt ggagtatttc gccgccgagg atctcgccga ggtcgaagcg
      241 cgaaccgcgt tggtgatccg cccttcgcga gcctgacccg ggcgcgaccg caccggctcc
      301 ccctcgccgc ttctcgaacg tcccgctact cgtaccgcag cgattcgacc gggtcgagcc
      361 gcgccgcgcg cgccgcgggg tacatcccga agaagagccc gatcgcggtc gccgatccga
      421 acgcgaggag gatcccccag atgggaacgc cgaacgagaa atgggcgagg aggttgacca
      481 gcttccccac gcagagtccg gccagaacgc cgatgactcc tccggcgccc gtcagggtca
      541 tggcctccac caggaactgc cagaggatgt cccgctcgcg tgcgccgagc gctttgcgga
      601 ccccgatctc gcgcgtccgc tcggtcacgg agacgagcat gatgttcatc acgccgatcc
      661 cgcccaccat gagcccgatc gaggagatca ccaccatcac gaggtagaag gcgccggtga
      721 tctggccgta gagatcgacc agcgacttgt cggtgaagac ggcgaagttg tccccctggt
      781 cgtaccgcac gtggcgctgc cggcggagca cgtcggagat ctgctcccgg gccacgtcga
      841 tcatctccgg ggagaccggc ttcgcgttga gaaccatctt gagcttcggc ccgaagtcct
      901 tctcgagcgt cgtgtgtggg acgaggacga gctcgtccat gctcatcccc aggaagtgcc
      961 cccgtggctc cgcgacgccg accaccatga agggctgggt gccgatcgcg atcgtccttc
     1021 ccaccgggtt cacatgcggg aacagagtct ccacgtgatc cacgcccagg atgcagacgg
     1081 cggatcggtg ctcgacctcc gcctcggtga acgcgcggcc gttgtcgacc gcgtagttgt
     1141 tggtcaccat gtaatcggga gacgttccga tcacgatggt cgggcgggtc tgggtctcac
     1201 cgttcctgag gcggagctcc gtgaagttga gcggcgccac cgcgagcacc gcgggagcgg
     1261 tgcggcggat cgcatccgcg tcctcgggcg tgaaccattt ccggcgcctc aggctgtcgg
     1321 gcatctcccc cacccacgcg cccggagcga aggggcggat gtagaggaca tcggtcccga
     1381 gcgaggcgag ctgggccttc atgctctggt tcagcccctc gatgatcgag atcatcgcca
     1441 tgatggccgt gatcccgatg atcacgccga gcaccgtgag gaacgagcgg agcttgttct
     1501 cgcggagcgc gaggaacgcg atcccgactc cctccctgac gtcgatggcc atcgactact
     1561 cctgcctgag cgcgacgatc gggtcgagcc gggccgcgcg cgtggcggga tagacgccga
     1621 agaagagtcc gaccgcggac gccacggtca ggctgatcgc gaccgaccag ggctggatcg
     1681 tggccgggag cggcgtgatc gtccggatca ggagcgcgat cgccactccc gcgagaaccc
     1741 ccagcaagcc gccgaagaac gcgagcacga cggcttcgct caggaactgg gacacgagat
     1801 cggatcgctt cgcccccacg gccttccgga tgccgatctc ccgcgtccgc tcggtcacgg
     1861 cgacgagcat gatgttcatg atcacgatcc cgcccaccac gagcgagatc gcgacgagcc
     1921 cgatcgtgcc cgcaaagatg ccgccggtca gggtctggta gagggccatg tacgtctcgg
     1981 ccgtcgagaa cgcgaagtcg tcgggggcgc cgagcgggac gtgccgcgcg gcgcggagca
     2041 ccacgcgcgc ctcgtcttcg aggcgatcga gggtgacgcc ggacgcggcg cccacgatga
     2101 tcgtcaggga ccggtgggag ccgtacatct tctcgaactg cccgagcggg atgaccacga
     2161 ggttgtcctg gctctggccc agcacgttcc cgcgcgcgac gagcgtgccc acgacgagga
     2221 gctcgtagcc cgacaccttg atcgactgcc cgatggggtc gatgcggggg aagagcgtct
     2281 ccgccacctc ggagccgatc acgcagacgg gacggcgcct ctggaagtcc atgtcctcga
     2341 gctcccgccc ctggtcggtc tcgtacttcc ccaccgccgg gtagccctcg gtggtcccga
     2401 ggatgcggac gttcttgagc tccgtgccgc gatagcggac cgccttgttc ccctccgcct
     2461 gcgccacgac gtgggaggcg agctgcatgc gggaggcgag gacttcggct tcgtacgacg
     2521 tgaggtcccg gcgcttggcc gcctcgcggt acgcctcgtc atccgtgatg aggccgaact
     2581 tgtcgatgta gacgacgttc aggccctggt cgaggatccg ctcggagacg tacttgttca
     2641 cgccgtcgat gatcgagacc accgcgatga cggacatgac cgcgacgacg tttccgatga
     2701 cggtgaggac cgtccgtagc ttgtgggctc ggagcgcccg aacggcgatc ttgaggttct
     2761 cggccagcgc ggcccaggct tcgcgccggg gccgcccgat gggggtcacg cgccgggcgt
     2821 cgcgcccgct ctggccacgg gccggtcgct ctcgatcttc ccgtcgcgaa gccggacgat
     2881 gcggtgcgcg cgtgcggcga cgtcctcctc gtgcgtaacc aggacgaccg tgttcccctt
     2941 ggcgtggatc tcgtccatca aggtgaggat ctcgatcccc gtgcgcgagt cgaggttccc
     3001 ggtgggctcg tccgcgagca cgagggacgg gttgttcacg agcgcgcgcg cgatcgcgac
     3061 gcgctgcctc tggccacccg agagctcgtt cggccggtgg tgcgtgcggt ctccgagacc
     3121 gaccatcgcg agcatcgcct tggcgcgctc gcggcgctcg gtcgccggcg cccccgagta
     3181 gatgagcggc agctccacgt tgtggagcgc atccgcgcgc ggaagcaggt tgaaggtctg
     3241 gaagacgaat ccgatctcct ggttccggat ccgcgcgagc tcgtcgtcgt cgcgctcgcg
     3301 gatctcggtt ccccggagct tgtacgagcc gctcgtcggc gtgtcgaggc agccgacgat
     3361 gttcatgagg gtcgacttgc ccgaccccga cggacccatg atcgccacgt actcgcccgt
     3421 catgatccgc aggctgacgc cgtccagcgc gttgacctcg gtcgtaccca tctcgtatgt
     3481 cttgcggacg tcggtgatgt cgatcaggac gccgttcgcg ctcactgcga ctcctttccc
     3541 ttcgccttct tcggctcttc gatcttcacg cgctggccgg gcttgagcga gcgcagcgtc
     3601 ttgtggggcc cgatcacgac cttctccccg ggcttgatct cgcccaatac ctcgaagtcg
     3661 gtgtcgctcg cgatcccggt cttcacgcgg cggaactccg ccttgccgtc cacgatcacg
     3721 aacaccccgc ggatctcctc ctgcttcgga cgctcgcccg acggcgtggc cttctccgcc
     3781 gtctccgcct gagcgccccc gcgcttcttc gtcttcttgg tcaccggagg cttgaggtcc
     3841 tcgggtgtgc ggatcacgac cgactggatc gggaccgcga gcacgctgtc ccggcgggcc
     3901 gtgcggatct cgacgtccgc ggtcatgccg ggacgcagga tcggcggcgg gttgtcgatc
     3961 gtgacgtcga ccttgaaatt ggtctcctgc tcctgcgtgt ccgctcccga gatcaggggc
     4021 gagttggcga tctgcgtgac gtggccgacg agggatgtgt ccgggagcgc gtccaccttg
     4081 acggtcgcct cctgtccgat ccggatcgtc gcgacgtccg tctcgtcgac gtccgcttcc
     4141 accttcatcc ggctcaggtc cgccaccgtg aggagcaccg tcccgggatt gttcatggtc
     4201 ccgatcaccg cgatctcgcc ccgctcgatc gtgagctggg tcaccgtgcc gttgatcggc
     4261 gagatgaagc gcgtcttccg gagattgtcg tgcgccgcct ggagcgtcga ttggaggcga
     4321 cgaacgtcct cgcgccccgc ctcgacccgc gactggtcgg tgctcgcggc cgttcgcgcc
     4381 tggtccagct cctcggccga aaccaggttc tgggtcgaga gcgcctcctt gcgcttcagc
     4441 acggactgcg actgggccag cgacgcttcc gcgagcctga gattggagcg cgcggcagcc
     4501 agggccgcgt tggcctcgcc cacctgcgct cgatactgcg cgtcgtcgat ctggagcagg
     4561 aactgcccct tccgcaccga gtccccttca tggatcggca gcgtgcggac ctcccccggc
     4621 acgttggcgc tcagcttgac ctgagtctcc ggctggacgc gccctggggc gcgcacggtt
     4681 gcggtgaccg tgcccttccg cacgtccccc gcctgcacgg cgatcttgcc tcccgccgag
     4741 cggttcaggt tcgcgaggac gatcacgagg aggaggacca cccccgcgac gatcaggatg
     4801 atccgcgtac ggcccttcat gaatgggtct cctgggttgt catcgggcta gtaccgcttc
     4861 tcgccggtgg cgaactcgag attcgcctcc gcgatgcgcg cgtccgccac ggcctcgatc
     4921 agccgctcgc gcgcgttggc caggttgacc tcggcggtca ggaggtcgag gtacgtgccc
     4981 gcacccagat cgtagcgccc tttcgagaat ttgtagtcct cctccgcgga ggcgagccct
     5041 tcgcgctcca cgtcgatccg ttgcacggct tcgcggaggt tgagccacgc ctcctgcacc
     5101 tgggccgcga cgtccagctc gcgctggcgg cgctgcgatt ccgcttcgag gagcgtcccc
     5161 ttcgcggagc ggacgtcccc ttcggtctgg aagccggtga agatcggcag cgagagccgc
     5221 acctggccct gccactcggt cgcataatgc tgcgtatcca cgctcgacgg cgcgcccgga
     5281 atggccacgt cgatgtcctc gatggtctga tcggtcttgg cccgggagac gccgaacgac
     5341 ccggtgacga ccgggagccg ctgggtctgc gccccgaaga cgcccgaatg cgcggcccgc
     5401 tccacggccc gcgcgctctc catgagcggg cgcgtgccca ccgcctcgcg gatggcctgc
     5461 gacgaatcgg gcaacacgac ctggtcatcg agcacggcat ccggcacgat gcgcagggtc
     5521 ggatccaccc ccaccacctg agcgagccgc gacgactgga tgagcacctg gttgtccgcg
     5581 cggatgtgat ccacctgggt gttggcgcgc cgtgcgcgcg ccttgaggag atctccacgc
     5641 gccacggtcc ccacctggag gagcgcctcc gagcgcttct cctcgtcccg cgcgagctgt
     5701 tcggtttgcg ctgcgacctg ggccagacgg atggccttga gcaggttgaa gtactgctgc
     5761 ttcacctgga agacgacatt gttccgggtc tcctgctcgt cgtgctcgga acccttgcgg
     5821 agatggtcca gccgcttctt cgcgccgatg gccggcccgc tcaggatgtt cgacgtcaca
     5881 tccgcacgga tcgcccctgt cctgatgtcg gtcgcgagcc ccgtggcaaa cacgttgctg
     5941 tctgggttgg cggacgtcgg agtggggatc gtcaccccga cgaacgacga cgtcttgtcg
     6001 ggaatgaaat agttgtacga agcccctgtc gtgagcgacg ggatgatgcc gctccaggcg
     6061 gagagggcag cgccgctcgc ctgcttgcgc cgtgcctcgg cattgcggac gtcggcgttg
     6121 ctgtgaaggg cggtctggat cgcctgggcc aggctccacc gttcccccgg aatcgtgatc
     6181 ggggccacga gcagactctc gaccggagtc gcgggagtga agcgcaccgt gtcgccgacc
     6241 gccggctgcg gggcggcggt cggaggctgc gcggccgggg tcgcgggccg taggagcacg
     6301 gccgcgagaa cgagcatcgg cgtccaactg cgcttcaggt tcatcccatt ctcctgggta
     6361 cggttcgaga cggtgagggc aagccttgga ccctatacat acgtcggctg gaagacgttg
     6421 gtttcacggt ggttctggtc agcgcagcgc cgacaggatg agaccgacgg cgaacatggg
     6481 aagaacggcc aggagcccga acgaaaactt cttcctggcg tagacggaaa taccgatggc
     6541 cacgacggcc gtgctccaga ggatgaatgg atcggtcgcc gcgtccagca tccgcccgaa
     6601 cggcccgatg tcgtccccga gcaggtttcc caatcccagc cggatatcga gcgtcccctt
     6661 gctccacgcg atcccggtgc ggatcgcctg gtccaggaga ccgatagcgg cgccgtgcgc
     6721 ttgtgcggag agatagtgcc ggtacgtgat cccgcccccc atcatgaggt tccccatgaa
     6781 gaaggcgacc ccggacccga ggaggatgat gaacgccatc gtcacgaggc ccccgacgcc
     6841 gataatcaca ggcgcagcag gcgactcgaa gaaggcccgc attttctcga gctgctcggg
     6901 aggagcccct ttcgcctcct gcttggcgac ggcgtcgttc gcgaccgccc ccgattggag
     6961 cacgagcatg gagaacgcga cctgggccac gacgacgaga atgagcggca gccagtagcg
     7021 gggatggggc ctcatcgatt cgaaggtctc tcgcggccgc acgaagacac cggcgactgc
     7081 tccgagcggg gacggttccg gcagttcgga tgcggcggtc gccacctcgg tcatgtcact
     7141 cctccgtcgt ccaggacgcg gttgagaagg aacgggaacg ggatcgccac tagagcggca
     7201 gcggcgtccc ggctcgagct caatgggtcg atcgcgtcca tggccgcgcg gatcagcgag
     7261 ttcgcgcgcg ccacccaggc gcgttggata ggtccacgct cgtcgaggtt gacgtgaagg
     7321 gtcgccaccc cgcggccggg aaaccgccgc gaggcgtcgg aggcgtggaa aacggatgcc
     7381 agcgaaggga gatcagctcc tggctgccgc ggctcgaggc gggcccgacc cttgaagggg
     7441 aggccagggc gactgcaggg aggacctcca tgaaacgctg gaacacgttc gaaatacgca
     7501 gcttgtacca aggcgacatg aagcgctcag cctaggcccc gtgtcgcgga ctgtcaagcg
     7561 gcgagcggag agcgctcgcg gggtcggcgg ggaccgaggt cctgccccgc gccgtcacgg
     7621 cctcacagcc ggaacgagaa aggcacctcg atccacaccg cgaccggctg gcggcccgcc
     7681 ctcgcgggct tgaacgtcca gcggtagagc gtctcttcag cgctctccga caggccaggg
     7741 atggcgcgga tcacggtgac cttgcgcact cgtccgtcga cgcccacgag cgcgtggagc
     7801 acgacgcggc catcgatccc gttctcgcgc gcccactcgg gataggcggg atcggggttg
     7861 tagatcggca caggcggctc gtcgaaggcg acgaaggttt cctccgatgg atccggatcc
     7921 accaccgctt gagccatggc tcgctcatcg tcccccggaa tcgacggttg gtttccggtc
     7981 tctccgcccc cgggagcgat gcctaccttc ggatccacga ccgcgggctc cgtcttcgga
     8041 tcgtccatga tcaacttcgg atcgatgctc ccgccatcgg tccgcaccgg cggtgtcgtc
     8101 cctacccggg gcagaggcgg cgtctccacg cgcggaggca ccaggtcgac ccgccctctc
     8161 gtgagcacga gcgcgtcctc gggatgctga gcctggatcc agaggatgca tccaagagcg
     8221 ctcagatgga cgagcgccga cagcgcgagc ccttgcttga cgttccggcg ggacgatcca
     8281 cgtgcgggga cggcctcacg actaccgaac ggattccagc cagacggagg gacggccatc
     8341 gcgttcatgg gcgctgacct ttcgagcccg gcaccgtccc gcgaaacggg acgttcacgc
     8401 gagggggacc caaacaacaa gcggcgg
//
LOCUS       DASTKC010000087         7119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_301690, whole genome shotgun sequence.
ACCESSION   DASTKC010000087 DASTKC010000000
VERSION     DASTKC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301690"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFR25_03740"
     CDS             <1..506
                     /locus_tag="VFR25_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9940191.1"
                     /translation="GAFTIRDLVKNALRMRPDRIVVGECRGGEALDMLQAMNTGHDGS
                     LTTIHSNGPRDALHRLENLVLMAGHSLPDKAIREQVASALQVIVHVSRISDGSRKVLS
                     ISEITGMEGPVVTMQEIFRFNRTGTRSDGKVEGRYEATGVVPHFVDQVRLAGTDLTGI
                     FERGAAR"
     gene            503..1459
                     /locus_tag="VFR25_03745"
     CDS             503..1459
                     /locus_tag="VFR25_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9940192.1"
                     /translation="MIYVFVAILVFAAVATLVTAMAQRRKEQVDQKLRERVGTNELGG
                     PAGTIALERDTRMSTIPFLDTFLKNMAVARRIELFLYQAGMSMRVATFVLLIAASAMA
                     AYLISFAIFGKVLNAVFFLGVGGFLPVFFVGQKKNARMRAFAEEFPDTLDLLVSALRA
                     GISFSGALQIVADESPEPVRSEFAIVVEEQALGLDLREALANMANRVDSLDLKFFVTA
                     VVLQRDTGGNLTEVLENTGKLIRDRFRILGDIMTFTAQGRLTGLILTCLPAGLGMFMA
                     LTAPDYFHKMWDHPNGRMILGFAFLMQMLGGLAIRKIVDIKV"
     gene            1464..2390
                     /locus_tag="VFR25_03750"
     CDS             1464..2390
                     /locus_tag="VFR25_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9940193.1"
                     /translation="MFLISTFLLALALGIGFLALYQILVRDRDPVTARLRDLRTRALA
                     ARPKLDAETPSPIAQLLASLGGFLPTGEDKEALQSGLVRAGIRSQSAPMVFLGAKAIF
                     AVTFAIGWIILNFALARPIGSIFFSGAVAGVVGFYTPTIWLWMRGEARRARIQQALPD
                     ALDLMVVCVEAGLGLTAAIERVGREIAIASPALSDEFLLVNQEIRTGLSRSDALRRLA
                     RRTGVEDLYALSAMLIQADKLGTSIAQSLRAHAESMRTKRRQRAEQQARKAGIKLAFP
                     LVFLIFPALLIVILGPAAIQLMQAIAAQNATH"
     gene            2657..3250
                     /locus_tag="VFR25_03755"
     CDS             2657..3250
                     /locus_tag="VFR25_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9940194.1"
                     /translation="MTPARPDRTGLTDEELVRLVRGGETDAFAEVVRRVQRPAYRLAF
                     RITRHAEDADDVVQESLVKAYQALDRFQLGRALSPWILTIVARTALSLLRQGKRRNAM
                     SLDDPGPEGQATLAERSADPAADPGLWERRLDVERAFARLSEEHRVILALRVEADLPY
                     AQIAETLDVPVGTVMSRLARAREALMEQMTQTKKDER"
     gene            3247..4398
                     /locus_tag="VFR25_03760"
     CDS             3247..4398
                     /locus_tag="VFR25_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9940195.1"
                     /translation="MSVEPLHPAPEELYAYRDGELSAERRAFVETHVTACRVCRERMD
                     EMSKLEASLRSHPDTVGDAYYERMQAQVMAKVHAAAPPSRAPIREVPLERRRDDVERE
                     ERRRGAPKLPWIAVGSAVSAMAAVVVVVVLLVRQGSMPQVAVLERSAPDAGKLGAVSD
                     TSEAGMRGRLAAPPAESPAAPRDDARAKERQLAETKTKQQPTDQVAVAPYAEEEKKDK
                     AAANEMDQKLAVRPESDLMAKRSASKDEARLQQAEPQVSMAAPQASLAPPAAGAGNRY
                     ESALSRFGLPPVWGPGVSDDLVLKAEPVFRNLYRTGGATTPLDSARVRLYLAEAARLR
                     AGAAPDSAAIDEIEHHYRRAIRLGSEDPATVSTAAQRLREFLSEIGATP"
     gene            complement(4418..5206)
                     /locus_tag="VFR25_03765"
     CDS             complement(4418..5206)
                     /locus_tag="VFR25_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9940196.1"
                     /translation="MRATLRNLSFRALRVWQRDADVYFTTWKTEFLPPLLEPILYVLA
                     FGLGLGQLIRHVEYNGRTLGYLEFMAPGIVAVAIMFWAFFESTFSSYVRMYYQKTFDA
                     ILATPLLVEDVILGELLWATSKSMIAATIMLGVLGALGLVSWPTGLWVVPIAFFGGLL
                     FSAVGLLFTSMVKTISQFNVPMFTIIMPMFTFGGTFFPIDILPRWALAVAWCLPLTHV
                     AFLTRSAVLGFATPHLWVSAAYVAGLSLVLAAWALARMKRRLVP"
     gene            complement(5207..6133)
                     /locus_tag="VFR25_03770"
     CDS             complement(5207..6133)
                     /locus_tag="VFR25_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940197.1"
                     /translation="MNGLPGTSAIQARDLCKSYAGMEAVRGVSFDVAPGEVVGFLGPN
                     GAGKTTTVRMISCFLPPTSGTATVYGVDVRTSPREVKRMLGVCPQEDNLDPDFDVLKN
                     LLAYGRYFGIPGREIRDRAERLLDFVQLSDRKKSGVQELSGGMKRRLVLARALLNEPR
                     VLILDEPTTGLDPQARHAIWTRVRSLRATGVTVLLTTHYMEEAAQLCDRVVVIDNGKI
                     LMEGSPADLVLREVGSTVVEAWNYERAFAEFLSTLPGRLEEVGDRLYYYPGADDHVER
                     LLDERFPGQERLIRRATLEDVFLKLTGRALRD"
     gene            6278..7057
                     /locus_tag="VFR25_03775"
     CDS             6278..7057
                     /locus_tag="VFR25_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HET9940198.1"
                     /translation="MVRRGFLLFAALVLAVTPMTLHAQTGTTPPATTPPASTPPATTP
                     PASTPPPATTTPVPATDTAPGAEAAKPTPKSAILAMPEGPPAELKKLAQLVGNWNSTV
                     HVYASPMAPESETAGKSNYGWAFGGMHLEGKHQMKMNGKPFDGRSTWGYDTQKKQYQM
                     IWTDTMMPSAFVYYGTFPTDNSLSLFTTYMIGDKPVTEKVDVQFPDANSYVLTIQNDM
                     SGSMQKVMEEKGKRAAGNATAKGKSTAKKSTASKSTTKKAG"
BASE COUNT         1195 a         2429 c         2417 g         1078 t
ORIGIN      
        1 agggagcgtt cacgattcgc gatctcgtca agaacgcgct ccgcatgcgg ccggaccgga
       61 tcgtggtcgg tgagtgccgc ggtggcgagg cgctcgacat gcttcaagca atgaacacgg
      121 gccacgacgg atcgctcacc acgatccact cgaacggccc gcgcgacgct ctgcaccgtc
      181 tcgagaacct ggtcctgatg gccggccact cgctgcccga caaggcgatc cgcgagcagg
      241 tcgcgtccgc gctccaggtc atcgtccatg tctcgcgcat ctccgacggc tcgcgcaagg
      301 tgctctcgat ctccgagatc accggcatgg aagggccggt cgtgaccatg caggagatct
      361 tccggttcaa ccgcaccggc acccggagcg acggcaaggt cgaagggcgc tatgaggcca
      421 ccggagtcgt accgcacttc gtcgaccagg tccgcctggc cggcaccgac ctgaccggca
      481 tcttcgagcg gggggccgcg cgatgatcta cgtcttcgtc gccatccttg tcttcgcggc
      541 cgtagccacg ctggtcacgg cgatggcgca gcggcgcaag gagcaagtgg accagaagct
      601 ccgggagcgc gtcggcacga acgagctcgg gggaccggct ggaaccatcg cgttggagcg
      661 ggacacgcgc atgagcacga tcccgttcct cgacacgttc ctcaagaaca tggcggtggc
      721 gcgacgcatc gagctcttcc tgtatcaggc tggaatgagc atgagggtcg cgaccttcgt
      781 ccttctgatc gcggcgtccg cgatggccgc ctatctgatc tcgttcgcga tcttcggcaa
      841 ggttctgaac gccgtcttct tcctcggggt cggcggcttc cttccggtct tcttcgtggg
      901 acagaagaag aacgcgcgca tgcgggcgtt cgccgaggag ttcccggaca cgctcgacct
      961 cctcgtgagc gcgctccgcg cgggcatctc gttctcgggt gcgctccaga tcgtcgccga
     1021 tgagtctccc gagcccgtcc ggtcggagtt cgcgatcgtg gtcgaggagc aggcgctcgg
     1081 tctcgatctg cgcgaggcgc tggccaacat ggcgaaccgg gtcgactcac tcgacctcaa
     1141 gttcttcgtg accgcggtcg tactgcagcg ggacacgggc ggcaacttga ccgaggtgct
     1201 cgagaacacg ggcaagctga tccgcgaccg gttccggatc ctcggcgaca tcatgacgtt
     1261 caccgcccag gggcggctca cgggcctcat cctgacctgt ctccccgcgg gcctcggtat
     1321 gttcatggcg ctcaccgcgc cggattactt ccacaagatg tgggaccacc cgaacggtcg
     1381 gatgattctc ggcttcgcgt tcctcatgca gatgttgggc gggctcgcga tccggaagat
     1441 cgtcgacatc aaggtgtgac gccatgttcc tgatctcgac cttcctgctc gcgctcgctc
     1501 tcggaatcgg gttcctcgct ctctaccaga tcttggtgcg ggaccgcgat cccgtcaccg
     1561 cacggctccg ggacctccgg acgcgcgccc tggcggcccg tcccaagctc gacgccgaaa
     1621 cgccttcgcc gatcgcacag ctcttggctt cgctcggcgg cttcctcccg accggcgagg
     1681 acaaggaggc gctccagtcg ggcctcgtgc gcgccggcat ccgctcgcag agcgcgccga
     1741 tggtcttcct cggagcaaag gcgatcttcg ctgtgacctt cgcgatcggc tggatcatcc
     1801 tcaacttcgc gctcgcgcgc cccatcggct cgatcttctt ctcgggtgcg gtcgcgggcg
     1861 tggtcggctt ctacacgccg acgatctggc tctggatgcg gggcgaggca cgccgcgccc
     1921 ggatccagca ggcgcttccc gacgcgctcg acctcatggt cgtctgcgtg gaggcgggac
     1981 tcggcttgac cgccgcgatc gagcgcgtgg ggcgcgagat cgcgattgcg agtccggctc
     2041 tgagcgacga gttcctgctc gtgaaccagg aaattcggac cgggctctcc cggtccgacg
     2101 cgctccggcg cctcgcgcgg cgcacgggcg tggaggatct ctacgcgctc tccgcgatgc
     2161 tgatccaggc cgacaagctc ggaaccagca tcgcccagtc gctccgcgcg cacgcggaat
     2221 ccatgcgcac gaagcggcgc cagcgcgcgg agcagcaagc gcgcaaggcc gggatcaagc
     2281 tcgccttccc gctcgtcttc ctgatcttcc cggcgctcct gatcgtgatc ttggggcccg
     2341 ccgcgatcca gctgatgcaa gcgatcgcag ctcagaacgc cacgcattag tggcgaacga
     2401 ggaggtgaat ttcacatgtg gaagcaaatg gtgctgtggg gactcgtggt cgcgttcggt
     2461 ctcatgtgga tgacgcgccg cggcaatcgt cgcaagaagg tcgaccgcta aggtcgactc
     2521 gagtcgtacc aggggcggac cgccacggcg gcccgcctct ttcgcttttg ggccgccgcg
     2581 taaccccacc cggattcgcc ccttccggtc gtttggaata gtcgtccggt gccgggagta
     2641 gtagagtcgg cgcccgatga cccctgcgcg gcctgatcga acggggctca cggacgagga
     2701 gctggtccgg ctcgtccggg gaggcgagac cgacgcgttc gcggaagtcg ttcgccgcgt
     2761 gcagcggcct gcctaccgac tcgcattccg gatcacccgc cacgccgagg acgcggacga
     2821 cgtcgtccag gagagcctcg tgaaggcgta ccaggcgctc gaccgcttcc agctcggccg
     2881 cgccctctcc ccgtggatcc tcacgatcgt ggcgcgaacc gcgctctcgc tcctcaggca
     2941 gggaaagcgc aggaacgcca tgtcgctcga cgatcccgga ccggaagggc aggccacgct
     3001 cgccgagcgg agcgccgatc ccgcagccga tccaggattg tgggagcgcc gcctggacgt
     3061 ggagcgcgca ttcgcgcgcc tgagcgagga gcaccgcgtg atcctggcgc tccgcgtgga
     3121 ggccgatctt ccctatgcac agatcgccga gaccctcgac gtgcccgtgg gcaccgtgat
     3181 gtcccggctc gcccgcgcgc gcgaggcgct gatggaacag atgacccaga cgaagaagga
     3241 cgagcgatga gcgtcgaacc gctccaccca gcgcccgagg agctctacgc ctaccgggac
     3301 ggcgagctgt ccgcggagcg gcgcgcgttc gtcgagactc acgtgacggc gtgccgcgtc
     3361 tgccgcgagc ggatggacga gatgtcgaag ctcgaggcct cgcttcggtc gcaccccgac
     3421 acggtggggg acgcctacta cgagcgcatg caggcgcagg tgatggccaa ggtccatgcg
     3481 gccgcaccgc catcgcgcgc gccgatccgg gaggtgccgc tcgagcgccg cagggacgac
     3541 gtcgagcgag aggagcggcg ccgcggcgcg ccgaagctcc cgtggatcgc ggtcgggtcc
     3601 gcggtcagcg ccatggccgc cgtggtggtc gtggtggtgc tcctcgtccg gcagggctcg
     3661 atgccgcagg tggccgtgct cgagcgctcg gcccccgacg cggggaagct gggcgcggtg
     3721 tccgacacga gcgaagcggg catgaggggt cggctcgccg ctccgcccgc ggaatcgccc
     3781 gcggcgcctc gcgacgatgc gcgcgcgaag gagcggcagc tcgcggagac caagacgaag
     3841 caacagccca cggatcaagt ggcggtcgcg ccgtacgcgg aagaggagaa gaaggacaag
     3901 gccgcggcga acgagatgga ccagaagctt gcggtgaggc cggaatcgga cctcatggcc
     3961 aagaggtccg cctcgaagga cgaagcgcgg ctgcagcagg cagagcccca ggtctcgatg
     4021 gcggcgccgc aggcttccct ggcgcccccc gccgccgggg cggggaatcg ctatgaatcc
     4081 gcgctctcgc gcttcggtct gccgcccgtc tggggacccg gggtgtccga cgatctcgtg
     4141 ctcaaggccg agcccgtctt ccgcaatctc taccggaccg gcggcgcgac gacgccgctc
     4201 gactccgcgc gggtccggct ctatctggcc gaggccgccc gactccgagc cggagccgcc
     4261 cccgattccg cggcgatcga cgagatcgag caccactacc gccgggcgat ccggctcggc
     4321 tccgaggacc cggccaccgt ctccacggcg gcgcagcgtc tccgcgagtt cctctccgag
     4381 atcggcgcca ctccctgacg ccgcgcgcgc gagcgcgcta cggaaccagc cgccgcttca
     4441 tgcgagcgag cgcccaggcc gcgagcacga gcgacaatcc cgcgacgtag gcagcactca
     4501 cccagaggtg cggtgtcgcg aaccccagaa ccgccgaccg cgtgaggaag gcgacgtgcg
     4561 tgagcggcag gcaccacgcg acggcgagcg cccagcgggg caggatgtcg atcgggaaga
     4621 acgttccgcc gaacgtgaac atcggcatga tgatcgtgaa catggggacg ttgaactggg
     4681 agatcgtctt caccatcgac gtgaagagga gcccgaccgc ggagaagagg agcccgccga
     4741 agaacgcgat cggaacgacc cagaggcccg tgggccacga cacgaggccg agcgcgccga
     4801 gcacgccgag catgatggtc gcggcgatca tggacttcga cgtcgcccag aggagctcgc
     4861 ccaggatgac gtcctcgacg aggagcgggg tcgcgaggat cgcgtcgaac gtcttctggt
     4921 agtacatgcg cacgtaggac gagaaggtcg actcgaagaa cgcccagaac atgatcgcga
     4981 ccgcgacgat gccgggcgcc atgaactcga gatagccgag cgtccgcccg ttgtactcga
     5041 cgtgccggat gagctgtccc agcccgagtc cgaacgcgag cacgtagagg atcggctcga
     5101 ggagcggcgg gaggaactcc gtcttccagg tggtgaagta gacgtccgcg tcccgctgcc
     5161 acacgcggag cgcgcggaac gagagattgc ggagcgtcgc gcgcatctag tcgcggagcg
     5221 cccgtccggt gagcttcagg aacacgtcct cgagcgtggc ccgccggatg agccgctcct
     5281 gtcccgggaa tcgctcgtcg aggagccgct ccacgtgatc gtccgcgccc gggtagtagt
     5341 agagccggtc gcccacttcc tcgagccgtc ccgggagagt cgagaggaat tcggcgaacg
     5401 cgcgctcgta gttccacgct tccaccaccg tgctcccgac ctcgcgcagg acgagatcgg
     5461 cgggcgaccc ctccatcaat attttcccgt tgtcgatcac gacgacgcgg tcgcagagct
     5521 gggcggcctc ctccatgtag tgcgtggtca ggagcaccgt gacgccggtc gcgcggaggc
     5581 tccgcacccg tgtccagatg gcgtggcgcg cctgcggatc gaggcccgtc gtgggctcgt
     5641 cgaggatcag gacgcgcggc tcgttcaaga gcgcgcgcgc gagcacgagg cgccgcttca
     5701 tgccgcccga gagctcctgc acgccgctct tcttccggtc cgagagctgc acgaagtcga
     5761 ggagcctctc ggctcggtcc cggatctccc gccccgggat gccgaagtag cggccgtagg
     5821 cgaggaggtt cttcaggacg tcgaagtcgg ggtcgaggtt gtcctcctgc ggacagacgc
     5881 cgagcatgcg cttcacctcg cgcgggctcg tgcgcacgtc gacgccgtac accgtcgcgg
     5941 tgccggacgt cggcgggaga aagcaggaga tcatccggac ggtggtcgtc ttgcccgcgc
     6001 cgttcggtcc gaggaatccg accacctcgc ccggcgcgac gtcgaatgag acgccccgca
     6061 cggcctccat gcccgcgtag ctcttgcaga gatcgcgcgc ctggatggca gatgttcccg
     6121 gaagcccgtt catgcagcga gtttagcacg cgccttaggg cggcccggca ggggcatgag
     6181 agcctcggcg gttgaagcgg tgctgcgcga gcggtaccct cccggggttc gagcatttgg
     6241 gggtcccgcc agttactctc attcccggag gcacaggatg gttcgccgtg gattcttgct
     6301 gttcgccgca ctcgttctcg ccgtcacacc catgaccctg cacgcgcaga cgggcaccac
     6361 gccgcctgcg acgacgccgc ccgcaagcac gccgcctgcg acgactccgc ccgcaagcac
     6421 gccgccaccc gcgaccacga ccccggtgcc tgcaaccgac acggctccgg gcgcggaagc
     6481 cgcgaagccc acgcccaagt ccgcgatcct cgcgatgccg gagggcccgc ccgccgagct
     6541 caagaagctc gcgcagctcg tcggcaactg gaactccacc gtgcacgtct acgcgagccc
     6601 gatggctccg gagtcggaga ccgcgggcaa gtcgaactac ggatgggcct tcggtgggat
     6661 gcacctcgag ggcaagcacc agatgaagat gaatgggaag cccttcgacg ggcgctccac
     6721 gtggggctac gacacgcaga agaagcagta ccagatgatc tggaccgaca cgatgatgcc
     6781 gtcggcgttc gtgtactacg gcacgttccc caccgacaac tctctctctc tcttcacgac
     6841 gtatatgatc ggggacaagc cggtgaccga gaaggtcgac gtccagttcc cggacgccaa
     6901 ctcctacgtc ctgacgatcc agaacgacat gtccggatcg atgcagaagg tgatggagga
     6961 gaagggcaag cgcgcggccg gaaacgcgac cgcgaagggg aagtcgacgg ccaagaagtc
     7021 cacggcctcg aaatccacca cgaagaaggc gggctgagcg ggtccgaaca aagaacgcga
     7081 aacgggcggt ccggcatggc gccgggccgc ccgtcgctt
//
LOCUS       DASTKC010000088         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_775401, whole genome shotgun sequence.
ACCESSION   DASTKC010000088 DASTKC010000000
VERSION     DASTKC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775401"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="VFR25_03780"
     CDS             <1..1836
                     /locus_tag="VFR25_03780"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940199.1"
                     /translation="PETMPPGDLPVPWEHPLVRQTIEDCLHEAMDVDGFLEVLRGLRD
                     GRIRRHAVDTREPSPFARGILNVMPYGFLDDAPLEERRTQAVSARRGLDPEIADTLGA
                     LDPDAVARVREQAWPEPESDEETHEALLWMGYVTEFEAEDSGWTEWIDSLRAGGRVVP
                     FQSLEGPAWRAVETVVDPVALVRGRMEALGPVVVLAPGEAPPPAPRGDERMRAIPFLP
                     AGAEASLLQLEVEGSVLRCRIEARRAFCDRRLLARIHRDTLERLRREIEPVTAAEFWR
                     FLTCWQHAADGYQLEGPRGVVEVVRQLAGFEIAAAEWEGAVLPSRVQGYRQEWLDQVT
                     LSGEVAWGRLWGGGRSPIRTTPICLVPREDLDPWLAVASGIPVSEPALAENDEGSLEQ
                     DGASAEPLRSSYAEVLLELLDRSGATFTRDLERRSGMLPSHVAMGLAELIAAGAITCD
                     SFGGIRRLITPPSRRRGVMKHAPFSPSGRWSRLRESRTESAGPVDPALVEFAATQLLA
                     RYGVVFRRVLDRERIPVPWRDLVRVYRLRELRGDVRGGRFVQRFAGEQYALPEAVDLM
                     RRLRRERADRGKGLVVSAADPLNLDGILTPEPRVPAQARRQVLVA"
     gene            complement(1811..>2931)
                     /locus_tag="VFR25_03785"
     CDS             complement(1811..>2931)
                     /locus_tag="VFR25_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9940200.1"
                     /translation="TLRWIGELIGFPAAEGILVSGGNMANIVAFYAARAAHADWPVRA
                     KGVAAGPQLRVYGSAETHTWIQKAADLSGIGEQAVRWIPVKRDQTMDLDALRAQVDAD
                     RAAGDRPFLVVGTAGSVSTGAIDPLPEIAAFCRERGLWFHVDGAYGGLAAALPDAPTA
                     LKGLSAADSIAVDPHKWLYAPLEAGCTLIREAGRLRSAFSYRPPYYHLDEEAINFLDL
                     GPQNSRGFRALKVWLMLRQAGASGYRRMIGDDIRLSRRMAEAIARRTDLELVTQVLSI
                     ATYRYVPEDLRARFGEEDVARYLDELNQSILERLQEEGEIFVSNAIVEGRYVLRACIV
                     NFRTQAADVDAVPEISARVGAEVDRAMRSKLRPPAPAA"
BASE COUNT          395 a         1025 c         1058 g          453 t
ORIGIN      
        1 cccgagacga tgccgcccgg cgatctcccg gttccctggg agcacccgct ggtgcgccag
       61 accatcgagg actgcctcca tgaggcgatg gacgtggacg ggttcctcga ggtcctgcgt
      121 ggtctccgcg acggccgtat ccgccgtcac gcggtcgaca cgcgcgagcc ctcgccgttt
      181 gcgcgcggca ttctcaacgt gatgccgtac ggattcctcg acgacgcgcc gctcgaggag
      241 cgccgcacgc aggcggtgag cgcgcgtcgc ggactcgatc ccgagatcgc ggacacgctc
      301 ggcgcgctcg acccggacgc cgtggcgcgc gtgcgcgagc aggcgtggcc ggagcccgag
      361 agcgacgagg agacgcacga ggcactcttg tggatgggct acgtcacgga attcgaggcg
      421 gaggattcgg ggtggacgga gtggatcgac tcgctccgcg cgggcggacg cgtcgtgccg
      481 ttccagagtc tcgagggtcc ggcctggcgc gcggtggaga cggtcgtgga tccggtggcg
      541 ctcgtccgcg ggcgaatgga agcgctcggt ccggtcgtcg tcctcgcgcc gggcgaggct
      601 ccacccccgg cgccccgcgg tgacgagcgc atgcgggcga ttccgttcct gcccgccggg
      661 gccgaggctt ccttgctgca gctcgaggtc gagggttcgg tgcttcgctg ccgcatcgaa
      721 gcgaggcgcg ccttctgcga ccggcgtctc ctcgcgcgca tccatcgcga tacgttggaa
      781 aggctgcgcc gcgagatcga gcccgtgacc gccgccgagt tctggcgctt cctcacgtgc
      841 tggcagcacg cggccgacgg ctaccaactc gagggcccgc gcggcgtcgt ggaagtcgtg
      901 cggcagctcg ccggattcga gatcgcggcg gccgagtggg agggggccgt gcttccctcc
      961 cgcgtccagg gatacaggca ggagtggctc gaccaggtca cgctctcggg cgaggttgcg
     1021 tggggacgcc tctggggagg agggcgctca ccgatccgga cgacgcccat ctgtctcgtg
     1081 ccgcgggagg atctcgatcc ttggctcgcg gtcgcgtccg gaattccggt gtcggagccg
     1141 gccctcgcgg agaacgacga ggggtcgctc gagcaggatg gcgcgtcggc ggagcctttg
     1201 cgctcgtcct acgcggaggt cctgctcgag ctcctcgacc ggagcggcgc cacgttcacg
     1261 cgcgatctcg agcgccgctc gggaatgctt ccgtcccacg tcgcgatggg actcgccgag
     1321 ctgatcgccg cgggcgccat cacctgcgat tccttcggcg gcatccggcg cctcatcacg
     1381 ccgccgagcc gccggcgcgg cgtgatgaag cacgctccgt tctcaccctc gggtcgctgg
     1441 tcacggctcc gcgaatcgcg gaccgaatcc gcagggccgg tcgatcccgc gctcgtcgag
     1501 ttcgcagcga cccagctcct cgcgcgctac ggcgtggtgt tccggcgcgt gctcgaccgc
     1561 gagcggattc cggttccctg gcgggatctc gtgcgggtct atcgactccg agaattgcgc
     1621 ggcgacgtgc gcggcgggcg attcgtgcag cgattcgcgg gcgagcagta cgcgctgccg
     1681 gaagcggtgg acctcatgcg ccggctgcgc cgcgagcgcg cggaccgcgg caaggggctc
     1741 gtggtgtccg cggccgatcc gctcaacctg gatggaatcc tgacccccga gccgcgcgtc
     1801 ccggcgcagg ctaggcggca ggtgctggtg gcctgagctt cgaccgcatc gcgcggtcga
     1861 cctccgctcc gacgcgcgcg ctgatctccg gcacggcatc cacgtccgcg gcctgcgtgc
     1921 ggaagttcac gatgcaggcc cggagcacgt accgcccctc gacgatcgcg ttcgagacga
     1981 agatctcccc ctcctcctgg agccgttcca agatggactg attcagctcg tccaggtatc
     2041 gcgccacgtc ctcttcccca aagcgcgccc ggagatcctc cgggacgtag cgatacgtgg
     2101 cgatgctcag gacctgcgtt acgagctcga ggtcggtgcg ccgcgcgatc gcctccgcca
     2161 tgcggcgcga gaggcggatg tcgtcgccga tcatgcgccg gtagcccgag gcgcccgcct
     2221 gccgcagcat cagccagacc ttgagcgcgc ggaatccgcg ggagttctgt ggtccgaggt
     2281 cgaggaagtt gatcgcctcc tcgtcgaggt gatagtaggg cgggcggtac gagaacgcgc
     2341 tccggaggcg ccccgcctcc cggatcaacg tgcatcccgc ttcgagcgga gcgtagagcc
     2401 acttgtgcgg atccaccgcg atcgagtcgg cggccgagag acccttcagg gccgtgggcg
     2461 cgtcgggcag cgcggcggcg agcccgccgt acgcgccgtc gacgtggaac cagagtcccc
     2521 gctcgcggca gaacgcggcg atctccggga gcggatcgat cgcgccggtg ctcaccgagc
     2581 cggcggtgcc gacgacgagg aagggccggt ctcctgccgc gcggtccgcg tccacctggg
     2641 cgcgaagcgc gtccagatcc atggtctgat cccgcttcac cgggatccat cgaaccgcct
     2701 gctcgccgat gcccgagaga tcggccgcct tctgaatcca ggtgtgcgtc tcggcggagc
     2761 cgtacacgcg gagctgtggg ccggcggcca cgcccttggc gcgcaccggc cagtccgcgt
     2821 gggccgcgcg cgccgcatag aacgcgacga tgttggccat gttgccgccg ctcacgagga
     2881 ttccctcggc cgctgggaat ccgatcagct ccccgatcca gcgcagagtc t
//
LOCUS       DASTKC010000089        12389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1011837, whole genome shotgun sequence.
ACCESSION   DASTKC010000089 DASTKC010000000
VERSION     DASTKC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011837"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFR25_03790"
     CDS             <1..528
                     /locus_tag="VFR25_03790"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9940201.1"
                     /translation="PSPGSVDVLLDVLKQESWYLRDQAARALATMGEAVVDRLIDMLE
                     VGLWYTRSAAASALGRMGLPVAASPLVALLRDPNRTVRDSARDALVHICGNEVGAFAV
                     ASAVHELAERAQRFAIDGLASRDPAAAERILAILADPGARAVAERRPAARAVNEDGVH
                     WDVVIGEEEPKQGTA"
     gene            597..1649
                     /locus_tag="VFR25_03795"
     CDS             597..1649
                     /locus_tag="VFR25_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9940202.1"
                     /translation="METLRRALPQGQWPRRERAPDDILLDRGAAILEACGARAVPPES
                     PTYPRGLRDLDKLERDDSLRPDPRVRRSKKLPTVFLRGSWSSERTVAIVGSRRATLDG
                     VRVARELAASLAASGIAVVSGLAQGIDAAAHEGALDAGGRSGAVLGTSIEASFPASHA
                     GLQERLADSLGIMTEVFPGSPSSSRSFSERNHLLAAISDLTLIVQGDGKSGALITTGA
                     ARAMERPVGAVPWDIYEALGAASHRLLHDGHAVLIRGPEDVLALLGLAPEVVPQAARP
                     SLPRLDGAESLALAAIGPVPRAFDEVAAASGLTAAELGAALLKLEILGLARREAGGRV
                     RRTPGVPSPGRSTGVA"
     gene            1651..2595
                     /locus_tag="VFR25_03800"
     CDS             1651..2595
                     /locus_tag="VFR25_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9940203.1"
                     /translation="MGRKRPFRPLRQAIEASALRTLGWIARSLSFERASDLGAFMGRA
                     AWRLLGRRRQIAIDNVTRALGPEPGGEPAEVIARRAVEQLGRTFVEFLALPAHSRESL
                     LARIELTGFGPARQWKEEGKGGVFLTGHFGNWELLGAAFGWTYGPVHYVLPRQTNPAS
                     DAYLNEIRRDLGITCHVIEDGMLAPMRVLKRGGFLGMLPDQDARKIGIHVPFFGRPAS
                     TLTGPARLAIAANVPIVIAMMERTGRGRFRAWVVRVLEPSPGADERSEIERLTRDINE
                     AFEDVIRARPDHWYWIHRRWKTPPPATEPAAAAALRRS"
     gene            complement(2582..2896)
                     /locus_tag="VFR25_03805"
     CDS             complement(2582..2896)
                     /locus_tag="VFR25_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HET9940204.1"
                     /translation="MEGKRSGPGGPPQLNMEIGEREAEGIYSNFVVISHSLSEFVLDF
                     ARVLPGTPKSKVFARVVMTPPNVRALLNALETNVKKYEESYGKIHTLDENQSKGIGFT
                     TS"
     gene            3066..5300
                     /locus_tag="VFR25_03810"
     CDS             3066..5300
                     /locus_tag="VFR25_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9940205.1"
                     /translation="MGVKRSSGAARVEEQLVLEWKRYAARRARSADKVAAGACYLESR
                     PCPFEARPDFETRFVQDCVHCARLHDAAERTGVAVPGSSGVLPTLPLLTRLLEEEGNG
                     LAMSAGGDGEDGRWRAAAVFLRALPLLHASSLSDRTARILLAAIARAYEDVVDTALFF
                     EVSSESSSLSLLAAFRHADLDVGLPASGGPIDVDALESTGAFDGAVFDRLREGPIPLD
                     QDRDLISDAVFEGRTAIVPRPSRELRLPALLVEHLPDAPVAILPVFGRERVRGLLVVS
                     AAAGIAGWTSDQMELLTAITSQTGIALEAAGLLDVARRRGTGLRALLGLATASLKTQR
                     PEILEAALSTLAASLEAPVGVAWTWQPRGTEAGPTRPDALPVTHGDPAAEEADLLEMG
                     VSLRHWLEADPRPIVIDDVRTDPRLPGTLPPKWLSALAVPLKSEEVVRGAVLLVRTGA
                     EGASPRPFPSDDVAIAELGASIVALVATRDHVEGAAARTERRVQELEAQLRHTDKMSV
                     VGERGIQVAQEIRNPIAAITGFARRALRTMEEGDPNREAIEIILRETDRLERILVEQV
                     SLAQMTRPRLKLQSLNALVQEVLELQSEELVRRRVRLLKRLSPEVPSLLLDGEKMRHV
                     LLHILQYALQQVPSGGRVRVETRTGTGHVQAEIAHDGPKTPGESLDRLFVPFSMSRRY
                     GAGVGLAVAYQIVREHGGEIRARSEGDWSSILTIYLPVRENQDRRGKPDRRAGRNDRR
                     RRLA"
     gene            5376..5451
                     /locus_tag="VFR25_03815"
     tRNA            5376..5451
                     /locus_tag="VFR25_03815"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5409..5411,aa:Phe,seq:gaa)
     gene            5495..5570
                     /locus_tag="VFR25_03820"
     tRNA            5495..5570
                     /locus_tag="VFR25_03820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5528..5530,aa:Thr,seq:cgt)
     gene            5671..7071
                     /gene="glmM"
                     /locus_tag="VFR25_03825"
     CDS             5671..7071
                     /gene="glmM"
                     /locus_tag="VFR25_03825"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9940206.1"
                     /translation="MTDPSRNDPLSGLMVSASGVRGIVGEALTPDVVLRVTAALGSFL
                     KPGPIVVGRDTRPTGAWISRFVHAVLVASGRDVVDVGIAPTPTVLFAIRHHDAAGGIA
                     VTASHNPAPWNALKLFGPGGTFLSPAQSEEVARRAREGGRSWARHDGIGAFLADDEAI
                     RRHRDAILALPALTPDRIRDRRFRVAVDAVNGAGSIAGPAILEALGCEVHAIHCTPNG
                     NFPRVAEPLPENLGDLGAEVKRTGAAVGFAYDPDADRLAIVDEAGRPIGEERTVQVAV
                     DWALARTPGLVVVNASTSMGVEAIAKRHGSTVLRSRVGEAHVAQLLLEREGTIGGEGN
                     GGVIYPALHATRDGILAAAITLDWLAQDARPLSARIAELPPLVMVKKTLPLRLNDAAA
                     LTEALARSFPDAERNVLDGEKYLWEDAWVQVRASGTEPIVRIMAEAGSRERADDLVRR
                     AADAVGRSQGSAANKA"
     gene            7086..8915
                     /gene="glmS"
                     /locus_tag="VFR25_03830"
     CDS             7086..8915
                     /gene="glmS"
                     /locus_tag="VFR25_03830"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9940207.1"
                     /translation="MCGIVGYVGPQESVPILLEGLRRLEYRGYDSAGVAILNGEGIKI
                     KKAAGKISVLEELLKSGGRPKGTLGIAHTRWATHGVPNTVNAHPHVDCKGRIAVVHNG
                     IIENYSTIKTKLAQEGHTFTTDTDTEVIAHLIEKFYDGNIEKAVAAAVRLLTGTYGIA
                     VICTDDPTKIVGARHGSPLVVGICDSEYILASDVSAIIRHTNQVVYLDDEEMVVITPA
                     GIHTSTIREEATTKKVETVDWDLEMIEKAGYPHFMLKEICEQPQSISNTLRGRLLAEE
                     GMARLGGLNMTAAELRAIQRVVITACGTSWHAGLIGEYLIEELARIPVEVEYASEFRY
                     RNPILEPGTIVLAISQSGETADTLAAMREAKRKGARVLGVCNVVGSTIARESDGGVYI
                     HAGPEIGVASTKAFTSQVAALALFTLYLGRLGELSPALGTELVRELEAIPGKIEKILA
                     GAESIRAIAKANSHHNNFLYLGRGVNFPVALEGALKLKEISYIHAEGYPAAEMKHGPI
                     ALIDDNMPVVFICTQDSAYEKVLSNMEEVRARKGKIIAVASEGDTHVGTKADHVLYIP
                     RTMGPLTPLLSAIPLQLLAYYIAVERGCDVDQPRNLAKSVTVE"
     gene            8912..9322
                     /locus_tag="VFR25_03835"
     CDS             8912..9322
                     /locus_tag="VFR25_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET9940208.1"
                     /translation="MSDAKVEAGRIVQTDSAPNAIGPYVQGRTGTLAGRWIVTSGQVG
                     IDPATQKLVSGGVVAETERAIQNLEAILKAGGGSLADIVKTTVFLADMAEFRAMNEVY
                     AKMMGEPFPSRSTIAVRELPLSARVEIEAWAFVA"
     gene            9340..10374
                     /gene="selD"
                     /locus_tag="VFR25_03840"
     CDS             9340..10374
                     /gene="selD"
                     /locus_tag="VFR25_03840"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:9376..9378,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET9940209.1"
                     /translation="MFRLTEQVSCAGUASKLGPEDLRQVLKGLPAPPRSGRVLVGPET
                     WDDAGVYKISPKLALVQTVDFLTPVVNDPQDYGKIAAANALSDVYAMGGKPVSALSIV
                     CFPEGGDLEILRLMLKGGAEMLRKAGVALLGGHSVRDREIKFGFAVTGEIHPKRVIAN
                     SGARKGDLLVLTKPLGIGILATALKRRLLPKATLALATRQMQQLNEAAAAAMQVERAT
                     AATDISGFGLLGHSLNIARASKKTIRIWSRAVPLLPGVLEFAASGVAPAGLHSNAAYL
                     APETRYDEAVPEPLRMALVDPQTSGGLLIAVAPSRVKTLLGRLKRARIQAAVIGEVLA
                     RAAAPIEVAA"
     gene            10381..10478
                     /locus_tag="VFR25_03845"
     tRNA            10381..10478
                     /locus_tag="VFR25_03845"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10415..10417,aa:Sec,seq:tca)
     gene            10489..11103
                     /locus_tag="VFR25_03850"
     CDS             10489..11103
                     /locus_tag="VFR25_03850"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HET9940210.1"
                     /translation="MDRPATGAKAYRLLLALAVVAVLAPGVARAQVPGASPGGVIVPP
                     PRDSVVVTPSVPQAPEPKKEKAVPDTTKHRSSPFKVMLRSAALPGWGQVYNGKLLKAG
                     VVVAGEGFLVYKALSELSKENDAVSRIDSSTPGTPEYDQAIEDRESHMNQKINYIWWT
                     VAVHLLQMADAYVDANLSDWSAEVGPDPAQAAGTRVALAYRVRF"
     gene            11114..>12389
                     /gene="lepA"
                     /locus_tag="VFR25_03855"
     CDS             11114..>12389
                     /gene="lepA"
                     /locus_tag="VFR25_03855"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET9940211.1"
                     /translation="MKHRQNVRNFCIIAHIDHGKSTLADRLLERTQTVPKSKMKDQVL
                     DNMDLEREKGITIKSHAIAMNYTAKDGKAYQLNLIDTPGHVDFTYEVSRSLAACEGAI
                     LVVDASQGVQAQTISNLFLALDQKLTIVPVLNKVDLPAAMPDEVSLEVAQLLHCDPDD
                     VVRVSAKEGRNIEGVLEAIVARVPPPEDRRDGTLQALIFDSVFDQFRGVIVYVRVMRG
                     VITPGETILFHSTNTRYEVLEVGVLRLGLEPRNELISGDVGYVIANVKVVADAKVGDT
                     ILDAGDPKPEPLPGYRPVKPMVFSGLYPMEAESYESLKESLARLQLNDASLTYEPETS
                     LALGFGFRCGFLGLLHMEIVQERLTREHNVNLIATVPSVEYHVLKTDGEVIIVENPAL
                     MPPAQQIESIEEPYVRAAVVVPIEYVGNIMKLA"
BASE COUNT         1924 a         4300 c         4276 g         1889 t
ORIGIN      
        1 ccctcccccg gctcggtgga tgtgctgctc gacgtgctga agcaagagag ctggtatctg
       61 cgcgatcagg cggcccgggc actggcgacg atgggtgagg cggtggtgga ccgcttgatc
      121 gacatgctcg aagtcggcct ctggtacacg cggagcgcgg cggcatccgc actcggtcgg
      181 atgggacttc ccgtcgcggc ctcgccgctc gttgcgctct tgcgcgatcc caaccgcacg
      241 gtgcgcgatt ccgcacgcga cgcgctcgtg catatctgcg ggaacgaggt gggagcgttc
      301 gcggtggcgt ccgcggtgca cgagctggcg gagcgcgcac agcgctttgc gatcgatgga
      361 ctcgcctcgc gggatcccgc ggccgcggaa cggatcctcg cgatcctcgc ggatccggga
      421 gccagagccg tcgccgaacg gcgacccgcg gcccgcgcgg tgaatgagga cggcgtccac
      481 tgggatgtcg tcatcggtga agaggaaccg aagcagggta ccgcctgaga tggggtgcgc
      541 cgggccccgc gcggcccggc gtctagacgt ggggggacgt ggtggcggtg gaagtcatgg
      601 agacgctccg gcgggctcta cctcagggac agtggcccag gagggaacgt gcgcccgacg
      661 acatcctcct cgatcgtggg gccgcgatcc tcgaggcctg cggtgcgcgc gcggtgccgc
      721 ccgaaagccc gacctatccg cggggtctcc gcgatctcga caagctcgag cgcgacgatt
      781 cccttcgacc tgacccgcgc gtccgccgct ccaagaagct tccgaccgtc ttccttcgtg
      841 gatcctggag ctccgagcgc accgtcgcca tcgtgggctc gcgccgcgcg accctcgacg
      901 gcgtccgcgt cgcaagggag ctcgcagcgt ccctcgccgc cagcggcatc gccgtggtga
      961 gcggactcgc gcagggaatc gacgccgccg cgcacgaggg agccctcgac gccggagggc
     1021 gaagcggcgc cgttctggga acgtcgatcg aggcttcttt tcccgcatct cacgccggtc
     1081 tccaagagag actcgccgac tcgctcggca tcatgacgga ggtcttcccg ggatcgcctt
     1141 cgagttcgcg ctcgttctcc gagcggaacc atctcctcgc cgcgatctcg gatctgaccc
     1201 tcatcgttca gggcgacggc aagagcggcg cgctcatcac gaccggcgcg gcccgcgcca
     1261 tggagcgccc ggtcggggcc gtgccgtggg acatctacga ggcgctgggc gccgcgagcc
     1321 atcgtctcct tcatgacggc cacgccgtct tgattcgcgg tcccgaggac gtgcttgcgc
     1381 ttctcggact cgcgcccgaa gtcgtccctc aggccgcgcg accgtcactc ccgcgactcg
     1441 atggagcgga atcgctcgcg ctcgccgcga tcggccccgt ccctcgcgcg ttcgacgagg
     1501 tcgccgccgc gtcgggcctc acggccgccg agctgggcgc cgcgctcctc aagctcgaga
     1561 tcctcggact cgcgcgacgc gaagcgggag ggcgcgtgcg gcgaaccccg ggcgtcccat
     1621 cgccgggtcg ttccaccggc gtggcctgac gtggggcgga agcggccgtt ccgtccgctg
     1681 cgccaggcca tcgaggcgag cgcgctccgc acgctgggat ggatcgcgcg gtccctctcc
     1741 ttcgagcgcg cgagcgatct gggcgccttc atgggacgcg cggcgtggcg tctcctcggc
     1801 cgccgccgcc agatcgcgat cgacaatgtc acgcgcgcgc tcggtccgga gccggggggc
     1861 gagccggccg aggtgatcgc gcgccgcgct gtggagcagc tcggccggac cttcgtcgag
     1921 ttcctggccc tccccgcgca ctcgcgagag tcgctcctcg ctcggatcga gctcaccggc
     1981 ttcggccccg cacggcagtg gaaggaggaa gggaagggcg gcgtcttcct caccggacat
     2041 ttcggaaact gggagctctt gggggcggcg ttcggatgga catacgggcc ggttcactac
     2101 gtgctgccgc ggcagaccaa tccggcgagc gacgcctacc tgaacgagat ccggcgcgat
     2161 ctcgggatca cctgccacgt catcgaggac gggatgctcg cgccgatgcg tgtgctcaag
     2221 cgcggcgggt tcctcggcat gctgcccgat caggacgcgc ggaagatcgg gatccacgtg
     2281 ccgttcttcg gacgtccggc gtcgacgctc acgggaccgg cgcgcctcgc gatcgcggcg
     2341 aacgttccca tcgtgatcgc gatgatggag cgaacggggc gcggccgctt ccgcgcgtgg
     2401 gtggtgcgcg tcctcgagcc gtcaccgggc gccgacgagc gctccgagat cgagcggctc
     2461 acgcgcgaca tcaacgaggc gttcgaggac gtgatccgcg cgcgtccgga tcactggtac
     2521 tggatccacc ggcggtggaa gacgccgccg cccgcgactg agcccgccgc tgccgcggcg
     2581 ctcaggaggt cgtgaagccg atccccttcg actggttctc gtcgagcgtg tggatcttgc
     2641 cgtacgactc ctcgtacttc ttcacgttcg tctcgagcgc gttcaggagc gcgcgcacgt
     2701 tgggcggcgt catcaccacc cgcgcgaaga ccttgctctt cggcgttccg ggcagcaccc
     2761 gcgcgaagtc gagcacgaac tccgacaggg agtggctgat cacgacgaag ttcgaataga
     2821 tcccctccgc ctcgcgctcg ccgatctcca tgttgagctg tggcggccct ccagggccgg
     2881 aacgcttgcc ttccattgat aatcctcctc ttttcaatga gttacatctg gacgctcacc
     2941 tcaagataag gccccaacct gccgtaaatc aattcgtatc aagggcggga gcctccgtgg
     3001 ctccgtcggg ccacgggccc accagccccg agcgctgccc acgacttgga accggagggg
     3061 aatggatggg cgtcaagcgg tcgtccggag cggcacgggt ggaagagcag ctcgtcctcg
     3121 agtggaagcg ctacgcggcc cgccgtgcgc gctcggccga caaggtcgcg gccggcgcgt
     3181 gctacctcga gtcccggccg tgcccgttcg aggcccgccc cgatttcgag acgcgcttcg
     3241 tccaggactg cgtccactgc gcgcgcctcc acgacgccgc cgagcggacg ggcgtcgcgg
     3301 tgcccggatc gtcgggcgtg ctcccgacct tgccgctcct gacccggctc ctcgaagagg
     3361 aaggcaatgg cctcgcgatg tccgcgggcg gggacggcga ggatggacgc tggcgcgccg
     3421 cggccgtctt cctgcgcgcg ctcccgctgc tccacgcgtc gtcgctttcg gatcgcaccg
     3481 cgcggatcct gctcgccgcg atcgcgcggg cgtacgagga cgtggtcgac acggccctct
     3541 tcttcgaggt gtcgtccgaa tcctcgtcgc tctcgctcct cgccgcgttc cgccacgccg
     3601 atctcgacgt gggtctcccc gcctccggag gtccgatcga cgtggacgcg ctggagtcga
     3661 ccggcgcgtt cgatggcgcg gtgttcgatc gcctgcgcga aggaccgatc ccactcgatc
     3721 aggatcgcga tctcatctcg gacgcggtct tcgaagggcg cacggcgatc gtgccgcgtc
     3781 cctcgcgcga gctcaggctc ccggccctcc tggtcgagca ccttccggat gcgccggtcg
     3841 cgatcctgcc ggtcttcggt cgtgagcgcg tccggggcct tctcgtggtc tcggcggcgg
     3901 cgggcatcgc cggatggacg tcggaccaaa tggagctcct caccgcgatc acgtcccaga
     3961 ccgggatcgc gctcgaggcg gcggggcttc tcgacgtggc gcgacgccgg ggaaccgggc
     4021 tccgcgcgct gctcgggctc gccaccgcgt cgctcaagac gcagcgccct gagattctcg
     4081 aggcggcgct ctcgacgctc gcggcgtcgc tggaagctcc cgtgggcgtc gcgtggacct
     4141 ggcagccgcg cggcaccgaa gcggggccga cgcgtcccga cgcgctaccc gtgactcacg
     4201 gagatcccgc ggcggaggag gccgaccttc tcgagatggg ggtctcgctc cgccactggc
     4261 tcgaggctga cccgaggccg atcgtgatcg acgacgtgcg gaccgatccc cgccttccgg
     4321 ggacgctgcc gcctaagtgg ctctcggcgc tcgcggtccc gctcaagtcg gaggaggtgg
     4381 tccgcggcgc cgtgctgctc gtccggaccg gcgcggaggg cgcgtcgccg cgtcccttcc
     4441 cgtccgacga cgtcgcgatc gcggagctcg gcgcgtcgat cgtcgcgctc gtcgcgacgc
     4501 gcgatcacgt ggagggcgcc gcggcgcgca ccgagcggcg cgtgcaggag ctcgaagcgc
     4561 agcttcgcca cacggacaag atgtcggtcg tcggcgagcg cggcatccag gtggcgcagg
     4621 agatccggaa cccgatcgca gcgatcacgg gattcgcgcg gcgcgcgctc cgcacgatgg
     4681 aagaggggga tccgaaccgg gaagcgatcg agatcatcct ccgcgagacc gaccgcctcg
     4741 agcggatcct ggtggagcag gtgtcactcg cgcagatgac gcgcccgagg ctcaagctcc
     4801 agagcctcaa cgcgctcgtg caggaagtgc tcgagctcca gtcggaggaa ttggtgcgcc
     4861 gccgcgtgcg gctcttgaag cgactctcgc ccgaagtgcc gagcctcctt ctcgacggcg
     4921 agaagatgag gcacgtgctc ctccacatcc tccagtacgc gctccagcag gtcccgagcg
     4981 gcggccgcgt gcgcgtcgag acccggaccg gaacgggaca cgtgcaggcc gagatcgcgc
     5041 acgacgggcc caagacgccg ggcgagagcc tcgaccgtct cttcgttccg ttctccatgt
     5101 cgcggcgcta tggcgcgggg gtggggctcg cggtcgccta ccagatcgtg cgggagcacg
     5161 gcggggagat ccgggcgcgg agcgaagggg actggagcag catcctcacc atttatctgc
     5221 cggtccgcga gaaccaggac cgccgcggca agcccgaccg gcgggccggc cgcaacgacc
     5281 gcagacgccg cctggcctag cgcctcccgg cgcgcccgga acccggtccc ggcgttgacc
     5341 ctcgcgcttc caccgtggta gagtcgtcgc ccggcgcgac cctagctcag ttggtagagc
     5401 accacactga aaatgtgggt gtccgcagtt cgattctgcg gggtcgcacc actttatgtc
     5461 tgcctgatcg cgtatcacgc gacttcgcta tcgggctggt gtagctcagc tggtagagca
     5521 cgtcactcgt aatgacgggg tcaccggttc gattccggtc accagctcca ctccccgggt
     5581 cggccggccc agaccctcgg ccactcacgg acgttcacga tcacgccgcg cgcccctcaa
     5641 ggggccgcgg cgcacgcacg gggccattcc atgaccgatc catcccgtaa cgacccgctc
     5701 tcggggctca tggtgagcgc gtccggcgtg cggggcatcg tcggggaggc cctgaccccc
     5761 gacgtcgtgc ttcgcgtgac ggcggcgctc ggctccttcc tgaaacccgg ccccatcgtc
     5821 gtcgggcggg acacgcgccc gaccggggcc tggatctccc ggttcgtcca cgcggtcctc
     5881 gtcgcctcgg ggcgggacgt cgtggacgtc gggattgcgc cgacccccac cgttctcttc
     5941 gccatccgcc accacgacgc tgccggtggc atcgcggtca ccgccagcca caaccccgct
     6001 ccctggaacg cgctcaagct cttcggtccg gggggaacct ttctcagccc ggcgcagtcc
     6061 gaagaggttg cacggcgggc gcgcgagggt gggcgctcct gggcgcggca cgacgggatc
     6121 ggcgccttcc tggccgatga cgaggcgatc cggcggcacc gggatgcgat cctcgcgctt
     6181 ccggcgctca ccccggatcg gattcgcgat cggcggttcc gggtcgccgt ggacgcggtc
     6241 aacggcgccg gatcgatcgc gggccccgcg atcctcgagg cgctcgggtg cgaggtgcac
     6301 gcgatccact gcacgccgaa cgggaacttc ccgcgcgtgg cggagccctt gcccgagaat
     6361 ctcggggacc tgggcgccga ggtgaagcgc accggcgccg cggtgggctt cgcgtacgat
     6421 ccggacgcgg accggctcgc gatcgtggac gaggcggggc gtccgatcgg cgaggagcgg
     6481 acggtccagg tcgcggtcga ctgggcgctc gcgcgcacgc cgggtctcgt ggtggtgaac
     6541 gcgtcgacct cgatgggggt cgaagcgatc gcgaagcggc acggatcgac cgtgcttcga
     6601 tcgcgcgtgg gcgaggcgca cgtcgcgcag ctcctcctcg agcgcgaggg gaccatcggc
     6661 ggtgaaggga acggcggcgt gatctatccc gcgctccacg cgacgcggga cgggatcctc
     6721 gcggccgcaa tcacgctcga ctggctcgcg caggacgcgc gtcccttgtc cgcgcggatc
     6781 gcggagctgc cgccgctcgt catggtgaag aagacgctgc cgcttcgtct taacgacgcc
     6841 gcggcgctca ccgaagcgct cgcgcgtagc ttcccggacg cggagcggaa tgtattggac
     6901 ggcgaaaagt acctctggga agatgcatgg gtacaagtgc gcgcatccgg gacggagccc
     6961 atcgtgcgca tcatggcgga ggcgggttcg cgcgagcgcg cggacgacct cgtccgccgc
     7021 gcggccgacg cggtcggccg ctctcaaggc agcgcagcga acaaagcctg aggggggctt
     7081 ctagcatgtg cggcatcgtt ggctatgtcg gtccgcagga gtctgtcccg atcctgctcg
     7141 aggggctccg ccggctcgaa tatcgcggct acgacagcgc gggcgtcgcg atcctgaacg
     7201 gcgaaggcat caagatcaag aaggccgccg gcaagatctc ggtgctcgaa gagctcctca
     7261 agtcgggggg ccggcccaag ggcacgctgg gcatcgcgca cacgcgctgg gccacccacg
     7321 gcgtcccgaa cacggtgaac gcccatcccc atgtcgactg caagggcagg atcgccgtcg
     7381 tccacaacgg catcatcgag aactactcca ccatcaagac caagctcgcg caggaagggc
     7441 acacgttcac cacggacacc gacaccgagg tgatcgccca cctgatcgag aagttctacg
     7501 acgggaacat cgagaaggcg gtcgcggcgg cggtgcgcct cctcacgggg acgtacggca
     7561 tcgcggtcat ctgcaccgac gatcccacca agatcgtggg cgcgcgccat gggagcccgc
     7621 tcgtcgtcgg gatctgcgac tccgagtaca tcctcgcgtc cgacgtgtcg gcgatcatcc
     7681 gccacacgaa ccaggtcgtg tacctggatg acgaggagat ggtcgtcatc acgccggcgg
     7741 ggatccatac gagcacgatc cgcgaggagg cgacgaccaa gaaggtcgag accgtcgatt
     7801 gggacctcga gatgatcgag aaggcgggct acccgcactt catgctcaag gagatctgcg
     7861 agcagccgca atcgatctcg aacacgctcc gcggccggct cctcgccgag gagggaatgg
     7921 cgcggctcgg cggtctcaac atgaccgcgg ccgagctccg cgcgatccag cgcgtcgtga
     7981 tcaccgcgtg cggcacgtcg tggcacgccg gattgatcgg cgaatacctt atcgaggagc
     8041 tcgcgcggat cccggtcgag gtcgagtacg cctccgagtt ccgctaccgg aatccgatcc
     8101 tcgagccggg cacgatcgtg ctcgccatca gccagtccgg agagacggcg gacacgctcg
     8161 ccgccatgcg cgaagcgaaa cgcaagggcg cccgggtgct cggcgtctgc aacgtggtcg
     8221 gctcgaccat cgcgcgggag tcggacggcg gcgtctacat ccacgcgggg ccggagatcg
     8281 gcgtcgcgtc gacgaaggcg ttcacatcgc aggtggcagc cctcgcgctc ttcacgctct
     8341 acctggggcg gctgggcgag ctctcgcccg cattgggaac ggagctcgtg cgcgagctcg
     8401 aggcgatccc ggggaagatc gagaagattt tggccggcgc cgagtccatc cgcgcgatcg
     8461 ccaaggcgaa cagccaccac aacaacttcc tctacctcgg acgcggcgtg aacttcccgg
     8521 tcgcgctcga gggggcgctc aagctcaagg agatctccta catccacgcc gagggctatc
     8581 ccgccgcgga gatgaagcac ggccccatcg cgctcatcga cgacaatatg ccggtcgtct
     8641 tcatctgcac gcaggactcg gcctacgaga aggtcctctc caacatggaa gaggtccgcg
     8701 cgcggaaggg gaagatcatc gcggtcgcgt ccgaggggga cacgcacgtc ggcacgaagg
     8761 cggatcacgt cctctacatc ccgcgcacca tggggccgct cacgccgctc ctctccgcga
     8821 tcccgctcca gctcctcgcg tactacatcg cggtcgagcg cggatgcgac gtggaccagc
     8881 cgcgcaatct cgcgaagagc gtgacggtcg agtgagcgac gcgaaggtcg aagccggccg
     8941 catcgtccag accgacagcg cgccgaatgc catcggcccc tacgtgcagg gacggaccgg
     9001 caccctcgcg gggcgatgga tcgtcacctc ggggcaggtc gggatcgacc ccgcaacgca
     9061 gaagctcgtg tccggcggcg tcgtcgccga gacggagcgc gccatccaga acctcgaagc
     9121 catcctcaag gcgggcggtg gatcgctcgc cgacatcgtg aagaccacgg tgttcctcgc
     9181 cgacatggcg gagttccgcg cgatgaacga ggtctacgcc aagatgatgg gggagccgtt
     9241 cccctcgcgc agcaccatcg ccgtccgcga gctgccgctc tccgcccggg tcgagatcga
     9301 ggcctgggcg ttcgtggcgt gagccacacc cgcgaggaca tcttccgcct caccgagcaa
     9361 gtctcctgcg ctggctgagc gtcgaagctg ggtccggagg acctgcgcca ggtgctgaaa
     9421 gggctcccgg ctccaccgag gagcgggcga gtgctggtcg gccccgaaac atgggacgat
     9481 gccggcgtgt acaagatctc gcccaagctc gctctcgtcc agacggtgga cttcctgacc
     9541 ccggtcgtga acgaccccca ggactatggc aagatcgccg cggccaacgc gctgagcgac
     9601 gtgtacgcga tgggcgggaa gcccgtgtcc gcgctctcga tcgtctgctt cccggaggga
     9661 ggcgacctcg agatcctgcg cctcatgctg aagggaggcg cggagatgct gcgcaaggcg
     9721 ggcgtggctc ttctcggcgg acattcggtg cgcgaccgcg agatcaagtt cggcttcgcg
     9781 gtgacggggg agatccatcc caagcgcgtg atcgcgaact cgggcgcgag gaagggggac
     9841 ctcctcgtgc tcacgaagcc gctcgggatc gggattctcg cgaccgcgct gaagcgccgg
     9901 ctcctgccga aggcgacgct ggctctggcg acgcgccaga tgcagcagct caacgaggcc
     9961 gcagcggccg cgatgcaggt cgagcgcgcg accgccgcga ccgacatctc gggcttcggc
    10021 ctgctcgggc actcgctcaa catcgcgcgc gcgagcaaga agacgatccg gatctggagc
    10081 cgcgcggtgc cgctcctgcc cggcgtgctc gagttcgcgg cgagcggcgt ggcgccggcc
    10141 gggctccatt ccaacgcggc gtacctggcc cccgaaacgc ggtacgatga ggccgttccc
    10201 gagccgcttc gcatggcgct cgtggatccg cagacttccg gagggctcct catcgccgtc
    10261 gcgccgtcgc gcgtgaagac tctcctcggc cgcctcaagc gcgcgcgcat ccaggcggcc
    10321 gtgatcggcg aagtgctcgc gcgcgccgcc gcgcccatcg aggtcgcggc ttgaccggaa
    10381 ggggacggat gggttctggt ggactccgag gacttcaaat ccttctgtcg ggcgtctctg
    10441 acgtccgagg tgggttcgat tcccacacgt tcccgccacg ccccgtgcgt ggatagaccc
    10501 gcgaccgggg ccaaggcgta ccgcctcctc ctcgcgctgg ccgtcgtcgc cgttctcgct
    10561 ccgggagtcg ctcgggcgca ggtgcccggc gcctcgccgg gcggtgtgat cgtgcctcct
    10621 ccgcgggact ccgtggtcgt cacgccgagc gtgccgcaag cgcccgagcc caagaaggag
    10681 aaggcggtcc ccgacacgac caagcaccgc tcgtccccgt tcaaggtgat gctccgctcg
    10741 gccgccctgc cggggtgggg tcaggtatac aatggcaagc tgctcaaggc cggcgtcgtg
    10801 gtggcgggcg aaggctttct cgtgtacaaa gcgttgtcgg agctgtcgaa ggagaacgac
    10861 gcggtctcgc gcatcgactc ctcgacgccg gggacgcccg aatacgatca ggccatcgaa
    10921 gaccgggaat cgcacatgaa tcagaagatc aactacatct ggtggaccgt cgcggtccat
    10981 ctcctccaaa tggccgacgc ctacgtcgac gccaacctct cggactggag cgccgaggtc
    11041 ggccccgacc cggcgcaagc cgcggggacg cgcgtcgccc tggcctatcg cgtccggttc
    11101 tgagccccac gacatgaagc atcgccagaa cgtccgcaac ttctgcatca tcgcgcacat
    11161 cgaccacggg aagtcgacgc tggcggaccg gctgctcgag cgaacccaga ccgtgcccaa
    11221 gtcgaagatg aaggaccagg tgctcgacaa catggacctg gagcgcgaga aggggatcac
    11281 gatcaagtcg cacgcgatcg cgatgaacta cacggcgaag gacggcaagg cctatcagct
    11341 caacctcatc gacacgccgg ggcacgtcga cttcacgtac gaagtatcgc ggagcctcgc
    11401 ggcgtgcgag ggggccatcc tggtcgtcga cgcgtcgcag ggcgtccagg cgcagaccat
    11461 ctcgaacctc ttcctcgcgc tcgaccagaa gctcacgatc gtgccggtcc tcaacaaggt
    11521 ggatctcccg gccgcgatgc cggacgaggt ctcgctcgag gtggcgcagc tccttcactg
    11581 cgatcccgac gacgtggtgc gggtctcggc caaggaaggg cgcaacatcg aaggcgtgct
    11641 cgaggcgatc gtcgccaggg tcccgcctcc cgaggatcgc cgcgatggaa cgctccaggc
    11701 gctcatcttc gattccgtct tcgatcagtt ccgcggcgtc atcgtctacg tgcgcgtgat
    11761 gcgcggggtg atcactcccg gcgagaccat cctcttccac tcgaccaaca cgcggtacga
    11821 ggtgctcgag gtgggcgtgc tccgcctcgg cctcgagccg aggaacgagc tgatctccgg
    11881 cgacgtgggc tacgtgatcg cgaacgtgaa ggtggtcgcg gacgcgaagg tgggggacac
    11941 gatcctcgac gcgggcgatc ccaagccgga gccgcttccc ggctaccgtc cggtcaagcc
    12001 gatggtcttc tcggggctct atccgatgga ggccgagagc tacgagtcgc tcaaggagtc
    12061 gctcgcgcgg ctccagctga acgacgcctc gctcacgtac gagcccgaga cgtcgctcgc
    12121 gctcggcttc gggttccggt gcgggttcct cgggctcctc cacatggaga tcgtccagga
    12181 gcgcctcacg cgcgagcaca acgtcaacct catcgcgacc gtcccgagcg tggagtacca
    12241 cgtgctcaag accgacggcg aggtgatcat cgtcgagaat cccgcgctca tgccgccggc
    12301 ccagcagatc gagtcgatcg aggagccgta cgtgcgtgcg gcggtcgtgg tccccatcga
    12361 gtacgtgggg aacatcatga agctcgccc
//
LOCUS       DASTKC010000090         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_68473, whole genome shotgun sequence.
ACCESSION   DASTKC010000090 DASTKC010000000
VERSION     DASTKC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68473"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFR25_03860"
     CDS             complement(<1..477)
                     /locus_tag="VFR25_03860"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9940212.1"
                     /translation="MSRILAANWKMNLDLAQARALYASCGEARAIYPDLTFLVFPPAT
                     ALATLREARGRTGPALGGQSCHSEPKGAFTGEVSAEQLRDAGAEYVLVGHSERRRLFC
                     ESDAVARAKLGAAWRAGLTPLLCIGEPLAERERQETQSVVSRQLARALDGAPARATL"
     gene            complement(474..1694)
                     /locus_tag="VFR25_03865"
     CDS             complement(474..1694)
                     /locus_tag="VFR25_03865"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9940213.1"
                     /translation="MSAPAFRRLSEIPVQGRRVFVRVDMNCPLDQEGKVGDDSRIVAS
                     LPTLRYLIEKGARVVVASHLGRPKGKPDPKQSLRPVREVLERYLRKPVAFAAPAVGAE
                     AEAASRALNDGDVLLLENLRFDPGEEKNDPAYAKALASLGDAYVDDAFGAAHRAHAST
                     EGITAFVADCAAGFLLEQELKSLGRLLESPERPYVAILGGAKISGKIDVLTNLLPRVD
                     RIVIGGGMMFTFLKAKGIEVGRSLVEEDRVETARKLLEDSEALKKLLLPSDCRAASGI
                     TGDDPGHVVPVGAIPEDQAGVDIGPASIHAIQDALAEARTIFWNGPMGIFEVPAYAEG
                     TLGVAQAVAQATRRGAFTVVGGGDSLAAVHQAGLASKISHLSTGGGASLEFLEGKTLP
                     GVAALERAARAEAR"
     gene            complement(1691..2689)
                     /gene="gap"
                     /locus_tag="VFR25_03870"
     CDS             complement(1691..2689)
                     /gene="gap"
                     /locus_tag="VFR25_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9940214.1"
                     /translation="MRVAINGFGRIGRLVLRAALKRQVEIDFVAVNDITDAKTLAHLL
                     HYDSVHGQWPERFSAEGSTITVGGKSIRALSEANPAALPWKDLGIDVVLECTGKFTDR
                     DKAACHIAAGARRVLVSAPAKGADATFVMGVNDSTYDPAKHTVVSIASCTTNCLAPVA
                     AVLDKTFGIDRGFMTTVHAYTSDQRLQDAPHKDLRRARSAALSMIPTSTGAAKAIGLV
                     LPTLQGRLDGISIRVPVATGSVVDLTVNVSKPASIQSVNDAMREAATGPLMGILQYNE
                     DPIVSSDIIGNPHSSIFDAPLTMVSGDRVVKVFSWYDNEWGFSNRMVDALLLLGKA"
     gene            complement(2698..3735)
                     /locus_tag="VFR25_03875"
     CDS             complement(2698..3735)
                     /locus_tag="VFR25_03875"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9940215.1"
                     /translation="MKVGVLTGGGDCPGLNAVIRAVVRRAITGHGWTVEGIRHGWRGM
                     LREEIEPLDLTTVSGILPRGGTILGTSRTNPYKEPDGEAVVRRTLEKRGIGALIAIGG
                     EDTLGAAAKLAKAGLPVVGVPKTIDNDVNGTDYTFGFDTALSIATEAIDRLHTTAESH
                     DRVMVVEVMGRHAGWIALGSGIAGGADMILIPEFPVRLAEIRDAIERRHERGKNFSII
                     VVAEGARLADDAGQPLHEQRDKRDEFGHIRLGGVGASLAERIEETTGYETRYSVLGHI
                     QRGGSPTAFDRVLGSRFGVSAVDLVARGGFGRMVAIRGTAIVDVPLAEALGSIKQVGP
                     ELYDMARVFFS"
     gene            complement(3880..5373)
                     /locus_tag="VFR25_03880"
     CDS             complement(3880..5373)
                     /locus_tag="VFR25_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HET9940216.1"
                     /translation="MRRPEPRSAGHPRLGLLGALLAAALFVPLAGRASSGDPATSTGE
                     LRFHAAATAFRHGEKDARADFSIRVPYRQIRFVPENDRYAAKLRITVEMWSKSGKRAG
                     YLQREAVLQSTDIYAVRDSLLGEIYTVGLAAPPGVYTYRVLVEDMNMDRMGFVYKMKG
                     QKRQGEVTGTIDMGPWLFKNPALSGLLFAWDVQPRSEGSPFARGPYDVMPQPSGYFGH
                     FQEAVSLYYELYDDPPPEGGATRLIETRIFAASGDTVFTSVDSLRISEGTGWPHAMSI
                     DTSEFPGGHYWITLRVKDERGVTAESRGEFDVLWSLDSWRPDASDLYEVTASTLMPSD
                     SVLVFRSLSMGDKERWVERLWRDADPTPDTADNEIRDEFLRRVNYANNHYSIFGRGMF
                     SDRGRVYIRYGEPDDVKIERVPVGAKTLGYNLDKIPLQSKTQITDVTDGTADDRAYEI
                     WTYDMKGKELVPRFGMNEISSGLKFVFVDQQGYGDYTLDYSSTTSIH"
BASE COUNT          844 a         1860 c         1897 g          895 t
ORIGIN      
        1 gagcgtcgcg cgtgccggcg caccgtcgag cgcgcgcgcg agctggcgcg agacgacgct
       61 ctgcgtctcc tgccgctccc gctccgccag gggctccccg atgcagagga gtggcgtgag
      121 tccggcgcgc caggcggcac ccagcttggc gcgtgccacg gcgtcgctct cgcagaagag
      181 ccgccggcgc tcggagtggc cgacgagcac gtactcggcg ccggcgtcgc gaagctgctc
      241 ggccgagacc tcgccggtga acgccccttt gggctccgag tgacagctct gaccgccgag
      301 cgcgggtccg gtgcggccgc gcgcttcgcg cagcgtcgcg agcgccgtgg ccggcggaaa
      361 gacgaggaac gtgagatccg gatagatcgc gcgcgcctcg ccgcacgacg cgtagagcgc
      421 gcgcgcctgt gcgaggtcga ggttcatctt ccagtttgcg gcgagaatgc ggctcatcgg
      481 gcctccgcgc gcgccgcgcg ctcgagcgcg gcgacgccgg gcaatgtctt cccctcgagg
      541 aattcgagcg acgcgccgcc gcccgtcgag aggtggctga tcttgctcgc gaggccggcc
      601 tggtgcacgg ccgcgagcga atcgccgccg ccaaccacag tgaaggcgcc gcggcgcgtg
      661 gcctgcgcca ccgcctgggc cacaccgagc gtcccttccg cgtacgccgg aacctcgaag
      721 atgcccatgg gtccgttcca gaagatcgtg cgcgcctccg cgagcgcgtc ctggatcgcg
      781 tggatcgacg cggggccgat gtcgacgccc gcctgatcct cgggaatcgc gccgaccggg
      841 acgacatggc ccggatcgtc ccctgtgatg cccgacgccg cgcggcagtc ggagggaagc
      901 aggagcttct tcagagcctc cgaatcctcg agcagcttgc gcgcggtctc gacgcgatcc
      961 tcctcgacga gcgagcgacc gacttcgatt cccttcgcct tcaagaacgt gaacatcatg
     1021 ccgccgccga tcacgatccg gtcgacacga gggagcaggt tcgtgaggac atcgatcttg
     1081 ccggagatct tggcgccgcc gaggatcgcg acgtacggcc gctccgggct ctcgaggaga
     1141 cggccgaggg acttcaattc ctgctcgagc aggaagccgg ccgcgcagtc cgcgacgaag
     1201 gcggtgatgc cctccgtcga ggcatgggcc cggtgcgcgg cgccgaatgc atcgtccacg
     1261 taggcgtcgc cgagcgacgc gagcgccttg gcgtaggcgg gatcgttctt ctcctcaccg
     1321 ggatcgaagc gcaggttctc gaggaggagc acgtcgccgt cgttcagtgc tcgggatgcg
     1381 gcctccgcct cggcgccgac cgctggcgcg gcgaaggcga ccggcttgcg aaggtagcgc
     1441 tcgagcacct cccgcacggg cctgaggctc tgtttcggat ccggctttcc cttcggacgc
     1501 ccgagatggg aggcaaccac cacacgcgct cccttttcga tcagataccg gagcgtcggg
     1561 agcgacgcga cgatgcgcga gtcgtcccct accttgccct cctggtcgag cgggcagttc
     1621 atgtccacgc ggacgaagac ccgccgcccc tgtacgggga tctcgctcag ccggcggaag
     1681 gcgggcgcgc tcacgccttg cccaggagaa ggagcgcgtc gaccatccgg ttcgagaagc
     1741 cccactcgtt gtcgtaccag gagaagacct tcacaacgcg gtcgcccgag accatggtca
     1801 agggcgcgtc gaagatcgag gagtgcggat tgccgatgat gtcggacgag acgatgggat
     1861 cctcgttgta ctggaggatg cccatcaagg ggcccgtggc cgcctcccgc atcgcatcgt
     1921 tcacgctctg gatgctcgcg ggcttggaca cgttcaccgt gaggtcgacc acggagccgg
     1981 tcgcgaccgg cacgcggatc gagatgccgt cgagccgccc ctggagcgtc gggaggacga
     2041 ggccgatcgc cttggcggcg cccgtcgagg tcgggatcat ggagagcgcg gccgagcgtg
     2101 cccggcggag atccttgtgc ggcgcatctt gaagtctctg gtcgctcgtg taggcgtgca
     2161 cggtcgtcat gaagcctcgg tcgatgccga aggtcttgtc gagcaccgcg gcgaccggcg
     2221 ccaggcagtt cgtcgtgcac gaggcgatcg agacgacggt gtgcttggcg gggtcgtacg
     2281 tggaatcgtt cacgcccatc acgaacgtgg cgtccgctcc cttggcgggc gcggagacga
     2341 gcacgcggcg cgcgccggcc gcgatgtggc acgcggcttt gtcgcgatcc gtgaacttgc
     2401 cggtgcactc gaggaccacg tcgatgccga ggtccttcca ggggagtgcc gcggggttcg
     2461 cctcggagag cgcgcggatc gacttcccgc cgaccgtgat ggtcgagcct tcggccgaga
     2521 agcgctccgg ccattgcccg tgcaccgagt cgtagtggag aaggtgcgcg agggtcttcg
     2581 cgtccgtgat gtcgttcacg gcgacgaagt cgatctcgac ctggcgcttg agcgccgccc
     2641 ggagcaccag gcggccgatc cgtccgaacc cgttgatcgc tacccgcacg aaccctccta
     2701 gctgaagaag acgcgggcca tgtcgtagag ctccggcccc acctgcttga tcgaaccgag
     2761 cgcttcggcg agcggcacgt cgacgatcgc ggttccacgg atcgccacca tccgcccgaa
     2821 cccgccgcgc gcgaccaggt ccacggcgga cacgccgaag cgcgatccga gcacgcggtc
     2881 gaacgcggtg ggcgacccgc ccctctggat gtggccgagc acgctgtagc gcgtctcgta
     2941 ccccgtggtc tcttcgatgc gctcggcgag cgaagccccc acgccgccca agcggatgtg
     3001 gccgaactcg tcgcgcttgt ccctctgctc gtggagcggc tgccccgcgt cgtcggcgag
     3061 ccgcgccccc tccgcgacca cgatgatact gaaattcttg ccccgctcgt ggcggcgctc
     3121 gatggcgtcc ctgatctccg cgaggcgcac cggaaactct ggaattagga tcatgtccgc
     3181 gccccccgcg atgcccgagc cgagcgcgat ccatccggca tgccgtccca tcacctccac
     3241 gaccatgacg cggtcgtgcg actcagccgt cgtgtggagc cggtcgatgg cctccgtcgc
     3301 gatcgagagg gcggtgtcga agccgaaggt gtagtcggtg ccgttgacgt cgttgtcgat
     3361 cgtcttcggg acgcccacga ccgggagtcc tgccttcgcg agcttggccg cagcgccgag
     3421 cgtgtcctcg cccccgatcg cgatcagggc gccgatgccc cgcttctcga gggtccggcg
     3481 gaccaccgct tccccgtccg gctccttgta tggattcgtg cgggacgtcc cgaggatcgt
     3541 gccgccgcga ggaaggattc ccgagacggt ggtcaggtcg aggggctcga tctcttcacg
     3601 cagcatccct ctccagccat ggcggatgcc ttcgacggtc cagccgtgcc ccgtgatggc
     3661 gcgccggacg acggcgcgga tgacggcgtt caatccgggg cagtcgcccc cgccggtcag
     3721 tacccccacc ttcatcgaac gcacccctcc gctcgcagca acgtccacat cgacaacgaa
     3781 ttagagcggc ggattgtagt gggtcggcgg gccctgcgtc aagagcggcg tccgggcggg
     3841 cgcagcgctg gaatccccgc ggctgaagcc gccaaggggt cagtggatgc tggtggtcga
     3901 cgagtagtcg agggtgtagt cgccgtagcc ctgctggtcc acgaagacga acttgagccc
     3961 cgaggagatc tcgttcatcc cgaaccgcgg aacgagctcc ttccccttca tgtcgtaggt
     4021 ccagatctcg tacgcgcggt cgtccgcggt cccgtccgtg acgtccgtga tctgggtctt
     4081 ggactgaagc gggatcttgt cgaggttgta gccgagcgtc ttggcgccga ccggcacccg
     4141 ctcgatcttc acgtcgtcgg gctcgccata acggatgtag acgcgaccgc gatcgctgaa
     4201 catgccgcgc ccgaagatcg agtagtgatt gtttgcgtag ttcactcgcc tcaggaactc
     4261 gtcccggatc tcgttgtccg ccgtgtccgg ggtcggatcc gcgtcacgcc agagccgctc
     4321 cacccagcgc tccttgtctc ccatcgagag cgagcggaag acgaggaccg aatccgaggg
     4381 catgagcgtg gacgccgtca cttcgtagag gtccgaagcg tcgggacgcc aggagtcgag
     4441 cgaccagagg acgtcgaact cgccgcgcga ttccgcggtg acgccgcgct cgtccttcac
     4501 gcggagggtg atccagtagt gccctcctgg gaactcggac gtgtcgatgc tcatcgcgtg
     4561 cggccagccg gtcccctcgc tgatgcggag cgagtcgacg ctcgtgaaca cggtgtcccc
     4621 cgacgcggcg aagatccgcg tctcgatcag cctcgtggcc ccgccctcgg gaggcggatc
     4681 gtcgtagagc tcgtagtaga gggagaccgc ttcctggaag tggccgaagt atccgctcgg
     4741 ctgcggcatc acgtcatacg gaccgcgtgc gaacggcgac ccctcggagc gcggttgcac
     4801 gtcccacgcg aagaggagcc cgctcagggc cgggttcttg aagagccagg gccccatgtc
     4861 gatcgttccc gtgacctcac cctgtcgctt ctgccccttc atcttgtaga cgaatcccat
     4921 ccggtccatg ttcatgtcct cgaccaggac gcgataggtg tagacgccgg gcggagccgc
     4981 cagcccgacg gtgtagatct cgccgagaag cgaatcgcgg acggcgtaga tgtcggtgct
     5041 ctggagaacg gcttcgcgct ggaggtatcc ggctctcttc cccgacttgc tccacatctc
     5101 gaccgtgatg cgcagcttgg cggcatagcg gtcgttttcg gggacgaagc ggatctggcg
     5161 gtacggaacg cggatcgaga agtcggcgcg ggcgtccttc tctccgtggc ggaaggcggt
     5221 cgcggcagcg tggaagcgga gctcgcctgt ggacgtggcg gggtcgccgg agctcgcccg
     5281 cccggccagc gggacgaaga gcgcggccgc gaggagcgcg cccagaagcc cgagccgcgg
     5341 atggcccgcc gatcggggtt ccggcctcct cacgggggcg ccccccgtcc tagaagctca
     5401 cgttcagcga aacgtggtgc gcgtcggaca ggaatttcat gtcctgatac gcgtagtcga
     5461 gtctcgcgac ggaggatttg atgacggtca gcggga
//
LOCUS       DASTKC010000091         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_778548, whole genome shotgun sequence.
ACCESSION   DASTKC010000091 DASTKC010000000
VERSION     DASTKC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778548"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1388)
                     /locus_tag="VFR25_03885"
     CDS             complement(180..1388)
                     /locus_tag="VFR25_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HET9940217.1"
                     /translation="MTRVPDLVPVRYGRMLVSPFAFLRGAAQLMADDLASTPVTGYRV
                     QACGDAHLANFGVFATPERNLVFDVNDFDETLPAPWEWDVKRLAASVAVAARNLNIEE
                     GARTAAVEGTVRAYRERMNELGPMSPLDVWYDRVDVQRVLAIAREERANDLKDALCVA
                     KVRHRTSLGALPKFTAIVEGRRRIVDKPPLIVHDAIEDRDAEKMLARYVESLPPDRRP
                     LLERWTIVDAARKVVGVGSVGTRCYLVLLMDHDGRTPLFLQLKEAKEAVTAPHAGPSE
                     FTHQGQRVVVGQRLMQAASDIFLGWTTDGDRHYYVRQFRDMKGSVNLDVMTESGFATY
                     ARLCGRVLARAHARSGDAAAIGSYLGRRRTFDEAVAVFADAYSYQTERDHAALVDAVR
                     SGRVTAVAPR"
     gene            complement(1788..>2085)
                     /locus_tag="VFR25_03890"
     CDS             complement(1788..>2085)
                     /locus_tag="VFR25_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940218.1"
                     /translation="GVRGSIPLSSTDEPPARQRFPGHMERAVEGPLGHPWHIYGTSAA
                     VRARLWRFVGGFQRRGEPVDRGALALRGRLGVDAEQHPWVLVPEELLGVANGGA"
BASE COUNT          333 a          729 c          696 g          327 t
ORIGIN      
        1 aacgagaccc tccagaatga cgaggaacgg gtagtccatg tcgccagcgc gatacagcac
       61 attgccgcgc ttccgttggc cgatgctcta cccattgcga ggggcgtgga tctgctcttc
      121 gggcaggcgt cggtgcacac cgatggtgtc cgacgtctcg acgtcgacgg ccaccgtgat
      181 caacgcggcg cgaccgccgt cacccgccct gagcggacgg cgtcgaccag cgcagcatgg
      241 tcgcgttcgg tctggtacga gtacgcgtcg gcgaacaccg ccacggcctc gtcgaacgtc
      301 cgccgccggc cgaggtagct gccgatcgcg gcggcgtcgc cagaccgggc gtgcgcccgt
      361 gcgagcacac gaccgcacaa tcgtgcgtag gtcgcgaatc ccgactctgt catgacgtcg
      421 aggttcacgg agcccttcat gtcccggaac tggcgaacgt agtagtggcg gtccccatcg
      481 gtggtccagc cgaggaagat gtcgctcgcg gcctgcatga gccgttgccc gaccacgacg
      541 cgctggccct ggtgcgtgaa ctcggacggc cctgcgtgcg gtgcggtcac agcctccttc
      601 gcttccttca gctgcaggaa cagcggcgtc cggccgtcgt ggtccatgag caacacgaga
      661 tagcaacggg tcccgacgct gccgacgccg accaccttgc gcgccgcgtc cacgatcgtc
      721 caacgctcga gtagcgggcg ccggtccgga gggagggact ccacgtaccg cgcgagcatc
      781 ttctccgcgt cacgatcctc gatcgcgtcg tggacgatga gcgggggttt gtcgacgatg
      841 cgacgccggc cttctacgat cgcagtgaac ttggggagcg cgccgaggct cgtccgatgg
      901 cgaaccttgg ccacacacaa cgcgtccttg aggtcgttgg cccgctcctc acgcgcgatg
      961 gccaggactc gttgcacgtc gacgcggtca taccagacgt cgagtggcga catcggtccg
     1021 agctcgttca tgcgctcccg gtacgcgcgc acggtgccct cgaccgccgc cgtgcgcgcc
     1081 ccttcctcga tgttgaggtt gcgagccgcc acggcgacgc tcgccgcgag gcgtttgacg
     1141 tcccattccc acggagccgg gagcgtttcg tcgaagtcgt tgacgtcgaa gacgaggttc
     1201 cgctcgggtg ttgcgaacac gccgaagttg gcgaggtgtg cgtcaccaca ggcctgcacg
     1261 cggtagcccg tcaccggggt gctggcaagg tcgtcggcca tgagctgcgc cgcaccgcga
     1321 aggaacgcga agggagagac cagcatccgc ccgtagcgca cgggcacgag gtccgggacg
     1381 cgggtcacgt tcgacgcctc gagacgcgcc actggatcgg gccgtcgggg atcgggctcc
     1441 caccgtgcgt gccctgaccg cggcacctcc tttcggagcg accggccctc gacgcagcgt
     1501 tgttcgtaag acttcgctcc ggcaggcggc atcatcctca aggatgacga ccacaccacg
     1561 tgcgcgcacc acgcgcccgc gtagtcagag cttgagcgaa ccacgcgact gggggtcaag
     1621 ggctccgggg caaaccggaa gcgttcgtct tccaggctcc ggccgggggt cctctgcgtc
     1681 gcgggctgtt ccgcgcccgg gtgtgggcgc cggcctgtcg aagggccggc ctcgagggcc
     1741 tcggcttcca cgacctccgc cgcaccaacg ccaccatgct catggcatca ggcgcctcca
     1801 ttcgcgacgc ccaggagctc ctcgggcaca aggacccacg gatgctgctc ggcgtctacg
     1861 ccaagtcgac ctcggagggc aagcgcgccg cggtcgaccg gctcaccgcg gcgctggaac
     1921 ccgccgacga acctccacaa ccgcgcgcgg acggcggcgg atgtgccata gatgtgccat
     1981 ggatgcccca gaggcccctc aaccgccctc tccatgtgcc ctggaaatcg ctgtctagca
     2041 gggggttcgt cagtggagct gaggggaatc gaacccctga cccct
//
LOCUS       DASTKC010000092         8032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1487535, whole genome shotgun sequence.
ACCESSION   DASTKC010000092 DASTKC010000000
VERSION     DASTKC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487535"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..919)
                     /locus_tag="VFR25_03895"
     CDS             complement(473..919)
                     /locus_tag="VFR25_03895"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET9940219.1"
                     /translation="MLEERLEQDMKSSLKAGEQTRLSTLRMMRSQILLEKKKSTTKPT
                     LSDDEVVAVLGAYAKKLRESAAEYEKLGRPDEAGKIKDELKTVQEYLPQPLSEDEARE
                     LVRQVLTELSITSTKDFGRAMKEVQARAKGRADGKLLSEIVRASLS"
     gene            1072..2595
                     /locus_tag="VFR25_03900"
     CDS             1072..2595
                     /locus_tag="VFR25_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9940220.1"
                     /translation="MASPWARAVRLPASVRTAVLAPLAILALLGVRTAAAVEERVWVS
                     AGPSVVFYDPKVALKDDLGISVSGTGYINRWLGIEGTYSTASPSREQPYTGDGSYSHF
                     GGGLIATPDRFAWTLPYLYAGIGSVSGKDLAGESKSHSSFHIGAGVLLRAGERLGFRL
                     DARDVTFKDDTFGDDQRVNIVQLTGSVTGLFMGRPRDTDEDGVPDKKDKSPETPKGAV
                     VDATGTPLDTDKDGVFDGLDKSPATPLGAKVDAMGVAIDSDGDGVADGIDTCDSTAVG
                     VVVNATGCPVDTDGDHVFDGPDKCPDTPAGALVDATGCPLDADGDGVADGIDICPNTP
                     TGTAVNTGGCPIQPSETARSLSEDYLITLPDLEFAAGGDSLEPAAMARLDDVGAALAQ
                     WPQAKIEIGVHVDDGPEPGFRIPLTQMRVRAVFSYLTRKYPSLNPKSFWLTGYGDTDP
                     IYPNTTAGNRAQNRRVEFRVMNLNTVYQERMKREAFGSSPAPPTPGLTPKPPSVPQG"
     gene            2679..5114
                     /locus_tag="VFR25_03905"
     CDS             2679..5114
                     /locus_tag="VFR25_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9940221.1"
                     /translation="MTAVALLVVLTMLFLATAAWLALRLRAAERVLRARESGESDRLH
                     TLFVHSPEGFWRADAEGKTLEINQAMADMLGVSISSVVGRPFGEFIPPGSQAIAQDAY
                     LRARAGEPVQFEADLRNAQKRELRVLVTTHPILDKDGRFVEGFGIIRDITERHRVESE
                     HRHAISLLEAALESTADGLLIVDREGRIVRFNERFARMWGIPESVLAARDDDRALASV
                     LSQLEEPELFLSKVRMLYAAPDAESFDTVRFRDGRVFERYSLPQRIAGGEIVGRVWSF
                     RDVTDRERAQAQRELAMEREATIARNLDSALFTVRVGPDGKLLRYDYISGGAEALYGV
                     SAGEMERDPTFWMRRVHPEDLKNVFEPAMALLLRLKPVMIEVRYETSRGIWRWHRSRL
                     TARIEEDGLLTIDGIETDVTERVQLEERFRHAQKMEAVGQLAGGVAHDFNNILTAVLG
                     YADLLLARLGPADPHRPALEEIKKGGERAAGLTRQLLAFSRRTPTQLISVDVNATIRG
                     VEPMLRRLVGEHLEFGLDLADGFGPVRADPVQLEQVIVNLVVNARDAMPGGGSIAIRT
                     DRVVLSEEEASLAEMAEGACVRLRVTDTGTGIAPSVLERIFEPFFTTKEPGRGTGLGL
                     ATVYGIVRQHRGNIRATSVEGRGSTFEVLLPESTGAPTTRRAAKPASDALPVGHERVL
                     LVEDNASLLTLARESLTELGYRVFAASNAVEALRILEVESGEMDILVTDVVMPEVGGR
                     ELARKVERLLPGMPILFVSGYVRDPEPMVDKDGRPVDYLEKPYTPMALARRVRSALDA
                     APARSAGGAGRAR"
     gene            complement(5085..6107)
                     /locus_tag="VFR25_03910"
     CDS             complement(5085..6107)
                     /locus_tag="VFR25_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9940222.1"
                     /translation="MRSHERPRQILLTGATGTVGRTLLPALAGLGAPVRALEHRAAPS
                     PAATRLAEFRSGDLERPASIRGLAEGCDVVVHAARRAGFMVLDRDRQRRIMIGGTEAM
                     LREARSAGASTFILIGYTGTVQERGPWMGAVDEETPPEGEYESESVRIQVEAEAMVLE
                     ANRAGSFRTMVVSPGVLASPRVPTALGELLGAFLRRELPYRLLDDVWIAVSEGGDVAR
                     CVAAAVTQGHGGRRYFATGECLTLGNIYERMSRLTGVPAPRRRIPDLLLEELGHLVPV
                     LPPHSFLRQIVLPRELVLHLTRLAPLTNARTRTELGFTPTPLDELILGFARTEGVLPR
                     TAGAAG"
     gene            6600..>8032
                     /locus_tag="VFR25_03915"
     CDS             6600..>8032
                     /locus_tag="VFR25_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940223.1"
                     /translation="MRQGHPAAWLAIGLALLMGAAYPKAAMAGPYTRLQVLMPGETAA
                     PGTGSGKTGTARGQVTGIPFTVTIRACDNTWNTVTTISNSIQITSTNATATLPAPQQL
                     TNGQMTVSVTFNAAGTFTVQAHDQTDNTIPDGNSAAVLCQVLDHFDFPTISQKHKYAG
                     VAEATTVTAKDPNNATVTAYNGPVSLREVTSFGEGRVSPETVTMTNGVWSGNVTLYRA
                     DETSINRGNANMEAYLASNPLKKGTSDPFIVHPGALSRLQVIVPGLTPLPGSVSGYTG
                     SPATQGVGTAFTVGVFGTDAYWNPVPSTNTVTISATGSFTSNPASGALVTQGSPSGGY
                     RSFQVTLNTVGTQTVSASAGGLTSGTSPSISVIPAGTHHFAFNTITGPITAGVPTPIT
                     IRAVDSGGNTVPTYAADAILTSNTGTGSIIPSQVTFSAGTWTGNVVFKGAGGAVALTC
                     ADYSAPPKTGTSNTFVVSPGPFAGLQVL"
BASE COUNT         1229 a         2846 c         2728 g         1229 t
ORIGIN      
        1 acgcggcccg cgcgaatcgc ggtacgggcg tcccggtccg gacggcccgc gatcgccgta
       61 cggcacgggg ccgcgcgcgc cgccctgcgg ccgcacgccg cccgacatcg ggcgcctccc
      121 gccgtagcga tcgccacgct ggccgcgcga attctccgcc gcgacctggc gcatcagctc
      181 gagctcctcg ggcgggatgc gcgtgccgta ctgcgggtgc tccctcggcg ggcccgaggg
      241 cggcgatccg gcgggccgcc ctcgcggtgg acgacggtcc cccggcttgg ctcccggtcc
      301 gcgtccgcgc ggaggacgat cggaggatga gtgctgcgac ggagacgagc tgctacgtgg
      361 gcctcggccg tggctgggcc cggggcgtgg accgcgtttg cgattgggcg gcattatgcc
      421 tcgcgtgatc ttccgatcgg atgggttctg gaatcgcttg ggcgattccg gctcaggaga
      481 gcgatgcgcg gacgatctcg ctcaaaagct tgccgtccgc tcttcctttg gcgcgggcct
      541 gcacctcttt catggcgcgc ccgaaatcct tcgtcgacgt gatggagagc tcggtcagga
      601 cctggcgcac cagctcgcgc gcttcatcct cggagagcgg ctggggcagg tactcctgca
      661 ccgtcttgag ctcgtccttg atcttgcccg cttcgtcggg ccgtcccagc ttctcgtact
      721 cggccgccga ctcgcggagc ttcttcgcgt aggcgccgag gacggcgacc acttcatcgt
      781 cggacaacgt cggcttcgtc gtcgacttct ttttctcgag gaggatctgg ctgcgcatca
      841 tgcgaagcgt ggagagccgc gtttgttccc ctgccttcag ggaggacttc atgtcctgtt
      901 cgaggcgttc ctccagcatg cgcgttcctc gggtgactcc tccgggcgag gccttatggc
      961 cgcctggatc gggacggacg ggcagtatag cagaatgttg acaagggcca tgaaaagcag
     1021 cattctgcgg ccgattctac ccttacaccg ccgtgtcccc ggaggaaccg catggcctcg
     1081 ccttgggccc gcgctgtccg tttgcccgct tcggttcgta ccgccgtcct cgccccactc
     1141 gccatcctcg cgctcctcgg cgtccgaacc gcggccgctg tcgaggagcg ggtatgggtt
     1201 tccgccgggc cgagcgtcgt cttctacgat ccgaaggtgg cgctcaagga cgatctgggg
     1261 atctccgtgt cgggcacggg ctacatcaac cggtggctgg gcatcgaggg aacctactcg
     1321 accgcgagcc cgtcccgcga gcagccgtac acgggcgacg gatcgtattc acacttcggc
     1381 ggcgggctga tcgcgactcc ggatcgcttc gcctggacgc tcccgtatct ctacgccggc
     1441 atcggctcgg tgagcggcaa ggatctggcc ggcgagtcca agtcgcactc ctcgttccac
     1501 atcggggccg gcgtcctcct ccgcgccggc gagcggctcg gattccgcct cgatgcgcgc
     1561 gacgtcacgt tcaaggacga cacgttcggg gacgaccagc gcgtgaacat cgtgcagctc
     1621 accggcagcg tgacgggcct cttcatgggc cgcccgcgcg acaccgacga agacggcgtg
     1681 ccggacaaga aggacaagag tccggagacg ccgaagggcg cggtggtcga cgcgaccgga
     1741 acgccgctcg acacggacaa ggatggcgtg ttcgacggac tcgacaagag cccggccacc
     1801 ccgctcggcg cgaaggtgga cgccatgggc gtcgcgatcg actccgacgg cgacggcgtg
     1861 gccgacggaa tcgacacgtg cgattcgacc gcggtcgggg tcgtggtgaa cgcgaccggc
     1921 tgtcccgtcg atacggacgg tgaccacgtg ttcgacggac ccgacaagtg ccccgacacg
     1981 cccgccggcg ccttggtgga cgccaccggt tgtccgctcg acgccgatgg cgacggcgtg
     2041 gccgacggca tcgacatctg ccccaatact ccgaccggca cggcggtgaa cacgggcggc
     2101 tgtccgatcc agccctcgga gacggcgcgc tcactgtccg aggactacct gatcaccctt
     2161 cccgatctcg agttcgcggc cgggggcgat tcgctcgagc ctgccgcgat ggcgcgcctc
     2221 gacgatgtcg gcgccgcgct cgcgcagtgg ccgcaggcca agatcgagat cggcgttcac
     2281 gtggacgacg ggccggagcc cggattccgg atcccgctca cgcagatgcg cgtgcgcgcg
     2341 gtgttctcct acctcacgcg caagtatccg tcgctgaatc cgaagtcctt ctggctgacg
     2401 ggctacggcg acacggatcc catctatccc aacacgacgg ccgggaaccg cgcgcagaac
     2461 cggcgggtcg agttccgcgt catgaacctg aacaccgtct atcaggagcg catgaagcgg
     2521 gaggccttcg gctcgagccc ggccccgccc acgccggggc tcacgcccaa gccgccctcc
     2581 gtccctcagg gttgagccca gggccaggaa gcgctacgct gcaggcggag taggctggca
     2641 ccgggggctc gccccccctc cgcacgtgtg aggtcacggt gaccgccgta gcgctcctcg
     2701 tcgtcctgac gatgctgttc ctggccacgg ccgcgtggct cgcgcttcgc ctccgcgccg
     2761 ccgaacgggt tcttcgtgcc cgcgagtccg gcgaatccga tcgtctgcac accctcttcg
     2821 tccactcccc cgaaggcttc tggcgcgccg acgccgaggg caagacgctc gagatcaacc
     2881 aagccatggc cgacatgctt ggggtttcta tatcgagcgt ggtcggacgg ccgttcggcg
     2941 agttcattcc gccgggatcc caggccatcg cgcaggacgc gtaccttcgc gcccgggccg
     3001 gggagccggt ccagttcgag gccgaccttc ggaatgcgca gaagcgcgag cttcgcgtgc
     3061 tcgtgaccac gcatcccatt ctcgacaagg acggccgctt cgtcgaagga ttcgggatca
     3121 tccgcgacat caccgagcgg catcgcgtgg agtcggagca tcggcacgcc atttcgctcc
     3181 tcgaggccgc cctcgaatcc accgcggacg gacttctcat cgtggaccgc gaagggcgga
     3241 tcgtacggtt caacgagcgt ttcgcccgca tgtggggcat tccggagagc gtcctcgcgg
     3301 cgcgggacga cgaccgggcg ctcgcgtcgg tgctctccca gctcgaggag ccggagctct
     3361 tcctctccaa ggtgcggatg ctctatgccg cgcccgacgc ggagagcttc gacaccgtcc
     3421 gcttccgtga cggacgcgtc ttcgagcgct attcccttcc ccagaggatc gccggggggg
     3481 agatcgtcgg ccgcgtctgg agcttccgcg acgtcaccga ccgggagcgc gcgcaggcac
     3541 agcgcgagct cgcgatggag cgcgaggcca cgatcgcgcg caacctggat tcggcgctct
     3601 tcacggtccg ggtcggcccg gacggaaagc tcctccgcta cgactacatc tcgggcggag
     3661 ccgaggcgct ctacggcgtg tcggcgggcg agatggagcg cgatcccacc ttctggatgc
     3721 gacgcgtcca tccggaggat ctcaagaacg tcttcgagcc ggcgatggcg ctgctcctcc
     3781 ggctcaagcc cgtgatgatc gaggtgcgct acgagacatc gcgcgggatc tggcggtggc
     3841 atcggtcccg gctcacggcg cgcatcgagg aggacggcct cctcacgatc gacggcatcg
     3901 agaccgacgt caccgagcgc gtgcagctcg aggagcggtt ccgccacgcg cagaagatgg
     3961 aagcggtggg acagctcgcg ggcggcgtcg cccacgactt caacaacatc ctgaccgcgg
     4021 tccttggcta cgccgatcta ctcctcgcgc ggctgggacc ggctgacccg caccgccccg
     4081 cgctcgagga gatcaagaag gggggcgagc gggccgcggg actcacgcgc cagctcctcg
     4141 cgttcagccg ccggacaccg acgcagctca tctccgtgga cgtgaacgcc accattcgcg
     4201 gcgtcgagcc gatgctccgc cgcctggtcg gcgagcacct cgagttcgga ctcgacctgg
     4261 cggacggatt cgggccggtg cgcgcggacc cggtccagct cgagcaggtg atcgtgaatc
     4321 tcgtcgtgaa cgcacgcgac gccatgcccg gaggcggctc gatcgcgatc cggaccgatc
     4381 gcgtcgtcct gagcgaggag gaagcgtcgc tcgcggagat ggccgagggg gcgtgtgtgc
     4441 gactccgcgt caccgacacc ggcaccggga tcgccccgtc cgtcctcgag cgcatcttcg
     4501 agcccttctt cacgaccaag gagccggggc gcggcacggg cctcggcctg gccaccgtct
     4561 atggcatcgt gcgccagcac cgcgggaaca tccgggcgac gagcgtggag ggccggggct
     4621 cgaccttcga ggtgctcctg cccgagtcga ccggcgcgcc cacgacgcgc cgcgccgcca
     4681 agccggcatc ggacgcgctc ccggtcggcc acgagcgcgt gctcctggtc gaggacaacg
     4741 cgagcctcct cacgctcgcg cgggagtcgc tgaccgagct cggctatcgc gtcttcgccg
     4801 cgtccaacgc ggtcgaagcg ctccggatcc tcgaagtgga atcgggcgag atggacatcc
     4861 tcgtcacgga cgtcgtgatg cccgaggtgg gaggacgcga gctggcccgc aaggtggaac
     4921 ggctcctccc cgggatgccg atcctcttcg tctcgggcta cgtgcgcgat cccgagccga
     4981 tggtcgacaa ggacgggcgc cccgtcgact acctggagaa gccgtacacg cccatggcgc
     5041 tggcgcggcg cgtccggagc gcgctggacg ccgcgcccgc ccggtcagcc ggcggcgccg
     5101 gccgtgcgcg gtagcacgcc ctccgtccgc gcgaatccca ggatcagctc gtcgagtgga
     5161 gtcggcgtga acccgagctc cgtccgcgtg cgcgcgttcg tgagcggagc gaggcgcgtc
     5221 aggtggagca ccagctctcg cgggagcacg atctggcgga ggaacgagtg gggcggcaga
     5281 accggcacga gatgccccag ctcctcgagg aggaggtccg ggatccgccg ccgtggcgcg
     5341 ggcactcccg tgagccgcga catgcgctcg tagatgttcc cgagcgtcag gcactccccc
     5401 gtggcgaagt agcgccggcc gccgtggccc tgagtcacgg ctgccgcgac gcatcgcgcc
     5461 acgtcgcccc cttcgctcac cgcgatccac acgtcgtcga ggagccggta cgggagctcg
     5521 cgacgtagga aggcgccgag gagctcgccg agcgcggtcg gcacgcgagg ggacgcgagc
     5581 acgccgggac tcacgaccat cgtgcggaag gatcccgccc ggttcgcctc gagcaccatg
     5641 gcctcggcct cgacctggat gcgaaccgac tccgattcgt actcgccttc gggaggcgtc
     5701 tcctcgtcca cggcccccat ccacgggcca cgctcctgca ccgtccccgt gtatccgatc
     5761 aggatgaagg tcgacgcgcc ggcgctccgc gcttcgcgaa gcatcgcctc ggtgccgccg
     5821 atcatgatgc gcctctggcg atcgcggtcg agcaccatga agcccgcgcg ccgcgccgcg
     5881 tgaacgacga cgtcgcatcc ttcggcgagg ccgcggatgc tggcgggacg ctcgaggtcg
     5941 cccgagcgga actcggcgag cctcgtcgcc gcgggcgacg gcgccgcgcg atgctcgagc
     6001 gcgcgcacgg gcgcgcccag ccccgcgagg gcgggcagga gcgtgcggcc cacggtgccg
     6061 gtggcgccgg tcagtaggat ctgtcgtggt ctctcgtggg atctcatcgt tggtcggagg
     6121 cgtcgcgacg cgacgctcga atcgccccct gacccatgac tctaccgtgc cggcgcgcgc
     6181 ggcgaaagag gcgtccgtca cgccgcgcct cgcgtcgcgc gccgcccgga ccggagcgcg
     6241 atccgcaccc gcctgagtcc ggacgggtgg cgttctgcaa gattcccggc cggcgggacg
     6301 ggcgcctcgc atcgcacctg ccggctcgat ccagccactg gggcggtttt cttgcgcgat
     6361 ggcgctttcg agccacgctc cgtgcgttcc gcgaacacgg ctcatctcct tgtgctccac
     6421 ccgagtcgcg tcactccgca tgcattcggt ggctcggccc ggcccctcgg cacaacgcct
     6481 gcaaggtagc ggggcgtcgg gaagcgtccg cccaggacgc tgacacccga ggctgaacgg
     6541 tgggaaccgt gacgcctcga cctttctttg ttcgaaacag tcatagcgga ggtgacgccg
     6601 tgcgccaggg acatccggca gcgtggctcg caatcgggct cgcgctcctc atgggggctg
     6661 cttatccgaa ggcggccatg gcaggtccgt acacgcgact tcaagtattg atgcccggcg
     6721 aaaccgccgc gcccggtacg gggagcggca agacgggaac cgcccgtggg caggttacgg
     6781 ggatcccgtt cacggtcacg atccgcgcct gtgacaacac ctggaacacg gtgaccacga
     6841 tcagcaactc gatccagatc acctcgacca acgcgacggc gacgctgccg gcgccgcaac
     6901 agctgaccaa tgggcagatg acggtgtcgg tcacgttcaa cgcggccggc acgttcaccg
     6961 tccaggccca cgaccagacc gacaacacga tcccggacgg caactcggcg gccgtgctct
     7021 gccaggtcct cgatcacttc gacttcccca cgatctccca gaagcacaag tacgcgggcg
     7081 tggcggaagc gacgacggtc acggcgaagg atccgaacaa cgccacggtg accgcctaca
     7141 acggaccggt cagccttcgc gaggtgacga gcttcggcga ggggcgcgtt tctcccgaga
     7201 ccgtcacgat gacgaacggc gtgtggagcg gaaacgtcac gctctaccgc gcggacgaga
     7261 cgagcatcaa ccgcgggaac gccaacatgg aggcgtacct cgcgtccaat cccttgaaga
     7321 aggggaccag cgatccgttc atcgttcacc ctggcgccct ttcgcgttta caggtgatcg
     7381 tgccggggct gacgccgctt ccgggcagcg tctccggcta cacgggctcg cccgcgaccc
     7441 agggggtcgg cacggcgttc acggtgggtg tgttcggaac ggacgcctac tggaacccgg
     7501 tgcccagcac gaacaccgtc acgatctccg cgacgggcag cttcacgtcc aaccccgcgt
     7561 cgggagcgct cgtgacgcag ggctcgccgt cgggaggcta ccgttcgttc caggtcacgc
     7621 tgaacaccgt cggcacccag accgtgtcgg cgagcgccgg cgggctcacg tcgggcacga
     7681 gcccctcgat ctcggtgatc cctgcgggca cgcaccactt cgcattcaac acgatcaccg
     7741 gcccgatcac ggccggcgtg ccgaccccga tcacaatccg cgcggtggac tccggcggca
     7801 atacggtgcc gacctacgcc gcggacgcga tcctcacgtc gaataccgga acaggcagca
     7861 tcataccgag ccaagtcacg ttctcggccg gcacctggac cgggaacgtc gtgttcaagg
     7921 gcgccggcgg cgcggtggcg ctcacgtgcg ccgactactc cgctcctccg aagaccggaa
     7981 cgagcaacac gttcgtggtg agccccggtc cgttcgccgg gctccaggtc ct
//
LOCUS       DASTKC010000093         6874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_306002, whole genome shotgun sequence.
ACCESSION   DASTKC010000093 DASTKC010000000
VERSION     DASTKC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306002"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFR25_03920"
     CDS             <1..577
                     /locus_tag="VFR25_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9940224.1"
                     /translation="QGLVDAAAAHGIDVDDQRFQAVIDHQARSESGPYRSYAEIAADS
                     LVHVLRMPKKAARTIAEEAGAWPLFPDAADGLRALMRIAPCVAMTNSDQSHGKQVQDQ
                     LGFRLNGWICAEDVRCYKPAPEFWTAVSSRRGVPFGRSWWHVSAYADYDMATAGRLGL
                     TTVFIERDHNVWGPSSLTAPDLRALAVMLAG"
     gene            complement(612..1586)
                     /locus_tag="VFR25_03925"
     CDS             complement(612..1586)
                     /locus_tag="VFR25_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940225.1"
                     /translation="MKHFLHHHKKSVAAAALHGAVLFVLVFAVVWGVTYGISQASSNA
                     PAMPTVVTPAAEAPVAVPDKQPVPAAWLGLSGSLRAVVDTPDALRSNAAFSLLSSDAQ
                     VSDPGVHPTELATPTGDSFYVVAMHPYDPQRGAVIGDYHVGSWPAPTGNPMYERPAGV
                     IEVTPDNEGTPVSEHFQLRDFLTHDQGAVWPKALVIRPKLVDKLELISQELAHRGLPN
                     KLHVMSGFRTPQYNALEVHPGGRARLSRHMYGDASDVFVDADGNGQMDDLNGDGKVTI
                     ADARVLFQVAESVEATHPDLIGGLSAYPATVAHGPFVHVDTRGKRARW"
     gene            complement(1725..2645)
                     /locus_tag="VFR25_03930"
     CDS             complement(1725..2645)
                     /locus_tag="VFR25_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET9940226.1"
                     /translation="MAIARDFLIWLSSKPSLTRPIATTGMRLGFARRFIAGETVEEAL
                     QVAQELNARGLLVVMNQLGEYVKSREEVEATFASYERLLREMAARKIVGTITVKPTQL
                     GLELAPDLCRELTSKLAAQAQAMGTVVEIDMEHSAVSGATVDLFEHVRRAYPNVGLAV
                     QSYLRRTASDLKRLEPYHPKIRLVKGAYNEPADVAFQEKREVDRSYSDLLGTLFANGF
                     YPAVATHDDALIARAKELARERSLRGDQWEVQMLFGVRRDLQEQLAREGVQMRVYVSF
                     GTEWVPYFMRRLAERPANVGFVLRALLTGK"
     gene            complement(2674..3876)
                     /locus_tag="VFR25_03935"
     CDS             complement(2674..3876)
                     /locus_tag="VFR25_03935"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET9940227.1"
                     /translation="MGTLARYVAEPYRPLFTLGILEAVAAALVWPLHAAGWIAYPAPL
                     HWTLMVQGFLASFVLGFMLTAYPQFHHTRKTEPWETLVVVALLLAVGVSAGFGIAAVP
                     QAGFLAALLFLITSILRRMPDRRGAPPEEFLFVLAGFLFGVVGSAWGLLVALGIAPEP
                     TPRAPLRLMTHGMMLSIVLGMGGLLVPTFSAMRQPLEIPGIARPGERRPRRFLYGALL
                     SLLAAAAVLESIGAAAPAAWLRALVGVPVLILVWKIARPLVRSDRVSTVLKLAGWCLA
                     AGLLLAAAAPTSLAGDHVLFLGGFGLLIQGIATRVVVAHGPWPRGDESRVLRWDSPAL
                     ILAAVLVRAVAELTAAKMPFFGIAGLLWVLAWILWGRRAFLRIARRPLPAPKPASLGR
                     SLSLSPSP"
     gene            complement(3876..4715)
                     /locus_tag="VFR25_03940"
     CDS             complement(3876..4715)
                     /locus_tag="VFR25_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9940228.1"
                     /translation="MRTDGSMHRILVIAREEYRRTIESRWLFLFSILFALVVLALSFF
                     GLAQSQEVGFQGFARVTLSLLNLSLFLVPLAALLLGVTSLSGDAGSLSLLLAQPVARG
                     EVLAGKFLGLGAALTVAQAVGFGGGGLVVAWNAGVAQAPAFLVLTFLSIALGWLSTSA
                     ALLVAAIWPDRLKAMSAALTLWLLMVVAYDLVILGATALLHGLPLQAVLLPALLVNPV
                     DLVRVLTTLAVGSGALLGPTSAVLVGFFGTAGGAALGLAVLLVETAAPLWMAGRIIHA
                     RDW"
     gene            complement(4715..5134)
                     /locus_tag="VFR25_03945"
     CDS             complement(4715..5134)
                     /locus_tag="VFR25_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940229.1"
                     /translation="MTRRYLVLLGSALVLGMALSVLAHSGWMRPGQAPAPATTTVARV
                     PVSIRVRDGVVTPGESVVPLGAKVAIEVVNDGHEDIELALSGYEGRLSTLHVAGATAA
                     RAEFTADRPGEDFAWLVDGRPAGRLRVAGSHLIEGHR"
     gene            complement(5131..5562)
                     /locus_tag="VFR25_03950"
     CDS             complement(5131..5562)
                     /locus_tag="VFR25_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940230.1"
                     /translation="MRSGHRALTGWLVTAALFLTIGCDRSEPPRVHVGAPCASCGMSI
                     RDTHFACVRGSRGHYRQFDSIECLLREGPARPGESIFLPDYDQAQLCLADTVWVVQGS
                     FPTPMGGGLAAFATEASARDVGARTNGRVGRLHEFIGRAKS"
     gene            complement(5555..6466)
                     /locus_tag="VFR25_03955"
     CDS             complement(5555..6466)
                     /locus_tag="VFR25_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940231.1"
                     /translation="MIRVEGIRKRFGAIVAVDGLDFVVEPGETFALIGPNGAGKTTTL
                     KMLLGLTRPDVGRVLLGPDRLPPHDPRARRALGYVPQRVEFAPARTVLEVLRFFADLK
                     GLERKAVEQALERVGLESHAARRAGELSGGYTQRLSLAQALLGDPSLLILDEPTGSLD
                     PEATWEFRSLVERLREEGKTIVLSSHLLTEVERIADRVLILLDGRAVQIERLDDLRQR
                     QLRASRLKIETKGEADAAEPVLRRLEVPFERSGSRELLVGASNGRSLEVLDALRLAGV
                     ELRSFHMQGPTLEETFLSVVRGGRRDA"
     gene            complement(6463..>6874)
                     /locus_tag="VFR25_03960"
     CDS             complement(6463..>6874)
                     /locus_tag="VFR25_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940232.1"
                     /translation="NDYPVALDMRHSDNRFDDGRSGNYWGENEAYDLNGDGLSDVPYS
                     PVSAFAFLSKQYPDLSLLAKSPAVAAIAVAERVFPSLRPSDIVDEHPLIRPAAEAKAP
                     SMTAHAAGPSWGSAAAFGGLLAVGIAGLAVRGRG"
BASE COUNT         1170 a         2350 c         2424 g          930 t
ORIGIN      
        1 tcagggcctc gtcgacgccg cggccgcgca cggcatcgac gtcgacgacc agcgtttcca
       61 ggcggtgatt gatcaccagg cgcgctccga gtcgggtccc taccggtcct atgcggagat
      121 cgcggcggac agcctggtcc acgtgctccg catgcccaag aaggccgcac gcacgatcgc
      181 cgaagaggcc ggggcgtggc ccctcttccc cgatgccgcc gacggattgc gcgcgctcat
      241 gcggatcgcc ccctgcgtcg cgatgacgaa cagcgatcag agccacggca agcaggtgca
      301 ggatcagctc ggattccggc tcaacggatg gatctgcgcc gaggacgtgc gctgctacaa
      361 gccggcgcct gaattctgga ccgccgtgtc gtcgagacgc ggagtgccgt tcgggaggag
      421 ctggtggcac gtctcggcgt acgccgatta cgacatggcc acggcgggac ggctcgggct
      481 cacgacggtc ttcatcgagc gcgaccataa cgtttggggg ccgtcatcgc tgacggcccc
      541 cgatctgcgt gcgctcgccg tgatgctcgc gggctagacg gcctcaccgc ccggcccgcg
      601 ctcatgcgcg attaccagcg cgcgcgcttc cctctcgtat ccacgtgcac gaacgggccg
      661 tgcgcgaccg tggcgggata ggcggagagt ccgccgatca ggtcggggtg ggtcgcctcc
      721 acgctctccg cgacctggaa gagaacccgc gcgtccgcga tcgtcacctt cccatcgccg
      781 ttcaggtcgt ccatctggcc gttcccatcc gcgtccacga acacgtccga cgcgtcgcca
      841 tacatgtggc ggctcaggcg cgcgcggccg cccgggtgaa cttcgagcgc gttgtactgc
      901 ggcgtgcgga acccggacat cacgtggagc ttgttcggca gtccccgatg cgcgagctcc
      961 tgggagatca gctcgagctt gtcgacgagc ttgggccgga tgacgagcgc tttcggccac
     1021 accgcgccct ggtcatgcgt gaggaaatcg cggagctgga agtgctcgga tacgggcgtg
     1081 ccttcgttgt ccggcgtgac ctcgatcacc cccgcgggcc gttcgtacat cggattcccc
     1141 gtgggggcgg gccagctccc gacgtgatag tccccgatca cggcgccacg ctgcggatcg
     1201 taggggtgca tcgcaaccac gtagaaggaa tccccggtcg gcgtcgcgag ctccgtcggg
     1261 tgaacccctg gatcggagac ctgggcgtcc gacgagagca gggaaaaggc cgcgttcgac
     1321 cggagcgcgt ccggcgtgtc gaccacggcg cggagactcc cggacagacc gagccacgcc
     1381 gccggcacgg gctgcttgtc cggaacggcc accggagcct cggctgccgg cgtcaccacg
     1441 gtcggcatcg cgggcgcgtt ggaggacgcc tgggagatcc cgtacgtcac gccccagacg
     1501 accgcgaaca cgaggacgaa cagaaccgcg ccgtgtagtg ccgcggcggc gaccgacttc
     1561 ttgtggtgat gcaggaagtg cttcatggga caccggcctt ccgaagattc acgatttcag
     1621 gcgaggggcc cgtccggagg caccggacag gggcgatcga caccagtagt ttggatggat
     1681 cccggggcct tggggttcaa ctaaatcgac ggtctgagga ttcgctattt ccctgtgaga
     1741 agggctctta gcacgaaccc cacgttggcc gggcgctccg cgagccgccg catgaaatag
     1801 ggcacccact cggtgccgaa cgagacgtag acacgcattt gaaccccctc ccgggcgagc
     1861 tgttcctgaa ggtcgcgccg gaccccgaac agcatctgga cctcccactg gtccccgcga
     1921 agagaccgtt cccgggccag ctccttggcc cgggcgatga gcgcatcgtc gtgggtcgcg
     1981 accgcgggat agaagccgtt ggcgaacagg gtgccgagga ggtccgagta gctccgatcg
     2041 acctcgcgct tctcctggaa cgcgacgtcg gccggctcgt tgtacgcccc cttcacgagg
     2101 cggatcttgg gatggtaggg ctcgagtcgc ttcaggtccg atgcggtgcg ccggagatac
     2161 gactgaacgg cgaggcccac attcgggtac gcccgcctca cgtgctcgaa gagatcgacc
     2221 gtcgcgccgc tcacggcgga gtgctccatg tcgatctcga ccaccgtgcc catggcctgc
     2281 gcctgcgcgg cgagcttgct tgtcagctcc cggcagagat cgggcgcgag ctcgaggccg
     2341 agctgggtcg gcttgaccgt gatcgtcccc acgatcttcc gcgctgccat ctcgcggagg
     2401 agccgctcgt acgatgcgaa cgtggcctcg acctcctccc ggctcttcac gtactcgccg
     2461 agctggttca tcacgacgag gagaccgcgc gcattgagct cctgcgcgac ctggagcgcc
     2521 tcttccacgg tctcgcccgc gatgaaccgg cgcgcgaagc cgaggcgcat tcccgtcgtg
     2581 gcgatgggac gcgtcaagga gggtttgctg gagagccaga tcaggaaatc tcgtgcgatc
     2641 gccatcgggc cgtactctac ccgatcggga cgcctacggc gacggcgaca aggacagcga
     2701 tcggccgaga ctggccggct tgggcgcggg gagtggacgc cgcgcgatgc ggagaaaggc
     2761 acggcgtccc cagaggatcc acgccaggac ccagagaagt cccgcgatcc caaagaatgg
     2821 catcttcgcc gcggtgagct ccgccacggc gcgaacgagc acggcggcga ggatgagcgc
     2881 gggtgaatcc caccggagca cgcgcgactc atctcctcgc ggccatggac cgtgcgccac
     2941 gacgacgcgc gtcgcgatgc cctgaatgag gagcccgaat ccaccgagga agagcacgtg
     3001 atcgccggcc agactcgtgg gcgccgcggc ggcgaggaga agtcccgccg cgaggcacca
     3061 gccggcgagc ttgagcacgg tcgaaacacg gtcgcttcgc accaggggac gcgcgatttt
     3121 ccagacgagg atcaggaccg gcacacccac gagcgctcgg agccacgcgg caggcgcggc
     3181 ggcgccgatg gattcgagga ctgcggcggc ggcgaggagc gagaggagcg cgccatagag
     3241 aaagcgtcgc ggccgccgct cgccagggcg agcgatgccc gggatctcga gcggctgtcg
     3301 catcgcgctg aacgtcggca ccagcaatcc gcccatcccg agcacgatcg agagcatcat
     3361 gccgtgtgtc atgagccgaa gcggcgcgcg cggcgtgggc tccggagcga tcccgagcgc
     3421 gaccaagagc ccccacgcgc tcccgacgac gccgaagagg aatcccgcga gcacgaacag
     3481 gaattcctcc ggaggcgcgc cgcgccggtc gggcatgcgc cgcaagatcg aggttatgag
     3541 gaagaggagc gctgcaagga acccggcctg cggcacggcc gcgatgccga accccgcgga
     3601 cactcctacg gcgagcagga gcgcgacgac gaccagcgtc tcccacggct ccgtctttct
     3661 cgtgtgatgg aactgcgggt aggccgtgag catgaacccg agcacgaacg aggcgaggaa
     3721 tccctggacc atgagcgtcc agtggagcgg cgcgggatac gcgatccatc ccgcggcgtg
     3781 gagcggccag acgagagcgg cggcgaccgc ctcgagaatg ccgagcgtga agaggggccg
     3841 gtagggctcc gcgacgtagc gcgcgagtgt gcccatcacc agtcccgcgc gtggatgatg
     3901 cgccccgcca tccagagcgg cgcggccgtc tcgacgagga gaaccgcgag gccgagggcc
     3961 gcgcctccgg ccgttccgaa gaacccgacg agcacggcgg aggtcgggcc gaggagcgcg
     4021 cccgatccca cggccagggt cgtgaggacg cgcacgaggt ccacgggatt gacgaggagc
     4081 gccggcagga gcacggcctg gagcggcaga ccgtggagga gcgcggtcgc gcccaggatg
     4141 acgagatcgt aggccaccac catcagaagc cagagcgtga gcgcggccga catcgctttc
     4201 aagcgatcgg gccagatcgc ggcgacgagg agggcggcgg acgttgagag ccatccgagc
     4261 gcgatcgaca ggaacgtgag cacgaggaac gcgggcgctt gcgcgacgcc tgcgttccac
     4321 gccacgacga gccctcctcc tccgaacccg accgcctgcg ccacggtgag ggcggccccg
     4381 agcccgagga acttcccggc gagcacttcc ccgcgggcga cgggctgggc caggaggagc
     4441 gacagcgacc ctgcgtcccc gctcaagctg gtcacgccga ggaggagcgc ggcgagcggg
     4501 acgaggaaga gtgagagatt gagaagactc agggtgacgc gcgcgaatcc ctggaagccc
     4561 acctcctggc tttgcgcgag gccgaagaag gagagcgcca ggacgacgag cgcgaagagg
     4621 atcgagaaga ggaagagcca gcgcgactcg atcgtgcgcc ggtactcctc gcgcgcgatg
     4681 accaggatgc gatgcatgga tccgtcggtg cgcatcaccg gtgcccctcg atgaggtggg
     4741 atcccgcgac ccggaggcgt ccggcggggc ggccgtccac cagccacgcg aaatcctcgc
     4801 ccggccggtc ggcggtgaac tccgcccgcg ccgcggtggc ccccgccacg tggagcgtgg
     4861 agagacgacc ttcgtagccc gagagcgcga gctcgatgtc ctcgtgcccg tcgttcacga
     4921 cctcgatcgc gaccttggcg ccgagcggca ccacggactc gcccggcgtg acgactccgt
     4981 ctcgaacgcg aatcgacacg ggcacgcgtg ccacggtggt ggtcgccggg gcgggcgctt
     5041 ggcccgggcg catccatccc gagtgagcca ggacgctgag cgccatcccg agcacgagcg
     5101 ccgagcccag gagaacgaga tagcggcgcg tcatgacttg gccctcccga tgaactcgtg
     5161 caagcgcccg acgcggccgt tcgtccgggc gccgacgtcc cgggccgatg cctcggtggc
     5221 gaacgcggcc aggccgccgc ccatcggcgt cgggaacgag ccctgcacca cccagaccgt
     5281 gtcggcgaga cagagctgcg cctgatcgta gtcgggaagg aagatcgatt cgccgggacg
     5341 cgcgggacct tcccggagca ggcactcgat cgagtcgaac tggcggtagt gtccgcggct
     5401 cccgcgcacg cacgcgaagt gcgtgtcccg gatgctcatc ccgcacgacg cgcacggcgc
     5461 gccgacgtgg acccgcggag gctcgctccg gtcgcaaccg atggtcagga agagcgccgc
     5521 cgtcacgagc caccccgtga gcgcccggtg accgctacgc atcgcggcgc cctccccgta
     5581 ccaccgacag aaacgtttcc tcgagcgtcg ggccctgcat gtggaaggag cgaagctcga
     5641 cgccggccag gcgcagtgcg tcgagcacct cgaggctccg tccgttcgac gctccgacga
     5701 ggagctcgcg cgaacccgac cgctcgaagg gaacctcgag ccgcctaagc accggctcgg
     5761 ccgcgtcggc ctcgcccttg gtctcgatct tgagccggct cgcgcggagc tggcgctgtc
     5821 ggagatcgtc gagcctctcg atctggactg cgcggccatc caggagaatg agcacgcggt
     5881 ccgcgatccg ctcgacctcg gtcaggaggt gcgagctcag cacgatggtc ttcccttcct
     5941 cgcgcagccg ctcgacgagc gatcggaact cccaggtcgc ctcgggatcg aggctccccg
     6001 tcggctcgtc gaggatgagg agcgacggat cgccgaggag cgcctgcgcg agcgagagcc
     6061 gctgcgtgta tccccccgag agctcgccag cgcgacgcgc ggcgtggctc tcgagaccga
     6121 cgcgctcgag cgcctgctcg accgctttcc gctcgagccc tttcaggtcc gcgaagaagc
     6181 ggagcacctc gagcaccgtg cgcgccggag cgaactcgac acgctgcggc acgtagccga
     6241 gcgcgcgacg cgcgcgtgga tcgtgcggcg gcaggcggtc cggcccgagg aggacgcgtc
     6301 cgacatcggg gcgcgtgagg ccgaggagca tcttgagggt cgtggtcttc cccgccccgt
     6361 tcggcccgat gagcgcgaag gtctctccgg gctccacgac gaagtcgagc ccgtcgacgg
     6421 ccacgatcgc gccgaagcgc tttcgaatgc cttcgacccg gatcatccgc gcccccgcac
     6481 ggcgagcccc gcgatgccga cggccaggag accgccgaac gcggcggcgg acccccagga
     6541 ggggccagcc gcgtgcgcgg tcatggaagg agccttcgct tcggccgcgg ggcggatcag
     6601 cggatgctcg tccacgatgt cgctgggccg aaggctaggg aacacccgct ccgccacggc
     6661 gatcgcggcg accgcggggc tcttcgcgag gagtgatagg tccggatact gcttcgacag
     6721 aaaggcgaac gcactcaccg gagagtacgg cacgtcgctc aggccgtcgc cgttcaggtc
     6781 gtacgcctcg ttctcgcccc agtagttccc ggaacggccg tcgtcgaagc ggttgtccga
     6841 gtgccgcatg tcgagcgcga ccggatagtc gttg
//
LOCUS       DASTKC010000094         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_70219, whole genome shotgun sequence.
ACCESSION   DASTKC010000094 DASTKC010000000
VERSION     DASTKC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70219"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..442
                     /locus_tag="VFR25_03965"
     CDS             113..442
                     /locus_tag="VFR25_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HET9940233.1"
                     /translation="MTPHDARAEQDTGAPEDEWVLKLYVAGQTPRSIAALENLKSICE
                     GHLGGKYTIEVIDLAQNPRLARTDQIVAVPTLVRKLPVPIRKIIGDLSNQQRVIVGLR
                     LDQNLNA"
     gene            459..2213
                     /locus_tag="VFR25_03970"
     CDS             459..2213
                     /locus_tag="VFR25_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940234.1"
                     /translation="MEPRTYRQLLADNEGLKAQLHHLRERLDEVEETIAAVRAGQVDA
                     LVVSAEPGEELYVLERIDRPYRLLLESMAHGAAVVDVDGRIAYCNRSLAELLRRSRDE
                     VTGSHWRDYLSGPDALRLADAMREVSERTSGRVEVLVQRPGASPQPIEIVIHPSPIRA
                     ISCFVITDLGPRRLIQDLVETRKRLSESDAEYRRLYAELREEERRKDEFLAVLAHELR
                     NPLAPLRNALHLLRTEPEREVAERARAIMERQLDSVVRLVDDILDVSRLMSGKVALRK
                     EPIELGSVFTRAVDVIRAQVEVEGQELIVALPEEPIRFEADEVRIAQAIGNILSNAVK
                     YTDKGGSIFLSGERDQDRIVIRIRDTGIGIPPDVLPRVFDLFVQADRSLTRSQGGLGI
                     GLTLVKRLVEMHGGRVQAASDGLGRGCEFTVTLPIASPSDIGGDDERRDVDVRAESRR
                     VLVVEDDSDSAQSLAMLLQIWGHHVEIAHDGHQALDAARSFGPEIVLLDIGIPGLDGY
                     EVAERLRSEHGAELKLIALTGYGREDDQQRSREAGFDRHITKPLEPPRLREMLASFDF
                     GTEGDADRPDRRSSAPLT"
BASE COUNT          454 a          882 c          864 g          395 t
ORIGIN      
        1 gcatcgatca ggagtcgtcg cggcttcgcg agctcgctct ggatcgacag gcgatgggag
       61 cgagacgcgg tgaggacggg ccctcggagg gcccgggagg gaacggagtg ccatgacacc
      121 tcacgacgca cgagccgaac aggacaccgg cgcaccggag gacgagtggg ttctcaagct
      181 ctacgtggcg ggccagaccc cacggtccat cgccgcgctc gagaatctga agtccatctg
      241 cgagggacat ctgggaggga agtacacgat cgaggtcatc gatctggcac agaacccacg
      301 actcgcgcgc accgaccaga tcgtggcggt ccccacgctc gtccgcaagc tccccgtgcc
      361 gatccggaag atcatcgggg atctctcgaa ccagcagcgc gtcatcgtgg gcttgagact
      421 ggaccagaac ctcaacgctt gaaccaggta ggacatcgat ggaaccgaga acgtatcgcc
      481 agctcctggc cgacaacgag ggcttgaagg cgcagctcca ccatctgcgc gagcggctgg
      541 atgaagtgga agagacgatc gccgcggtcc gtgcggggca ggtcgacgcg ctcgtggtgt
      601 cggccgagcc cggcgaggag ctctatgtcc tcgagcggat cgaccgcccc taccggctcc
      661 ttctcgagtc gatggcgcac ggcgcagccg tcgtggacgt ggacggccgg atcgcctact
      721 gcaaccggtc gctcgccgag ctcttgcgcc gctcgcgcga cgaagtgacg ggctcccact
      781 ggagagatta cctctccggt ccggacgcgc tccggctcgc cgacgccatg cgggaggtct
      841 ccgagcgcac gagtggacgc gtcgaggtcc tggtccagcg tccgggggcc agcccgcagc
      901 cgatcgagat cgtcatccac ccctcgccga tccgcgccat ctcctgcttc gtgatcacgg
      961 atctcggccc gcgccgtctc atccaggatc tcgtcgagac ccggaaacgc ctttcggagt
     1021 ccgacgccga ataccggagg ctctacgcgg aattgcgcga ggaggagcgg cggaaggacg
     1081 agttcctcgc cgtcctcgcg cacgagctca ggaatccgct cgcgccgctt cgaaacgcgc
     1141 tccatctcct gcgcaccgag ccggagcggg aggtggccga gcgggcccgc gcgatcatgg
     1201 agcgacagct cgacagcgtc gtccggctcg tggacgacat tctcgacgtg tcgcgcctca
     1261 tgagcggcaa ggtcgcgctc cggaaggagc cgatcgagct cgggtcggtc ttcacgcggg
     1321 cggtcgacgt gatacgcgcc caggtcgagg tggaagggca ggagctgatc gtggcccttc
     1381 cggaagagcc gatccggttc gaagcggacg aggttcgcat cgcccaggcg atcggcaaca
     1441 tcctctcgaa cgccgtgaag tacaccgaca aggggggcag catcttcctg agcggtgaac
     1501 gcgaccagga ccgcatcgtg atccggatcc gcgacaccgg catcgggatc cccccggacg
     1561 tgctgccgcg cgtgttcgat ctcttcgtgc aggcggaccg ctcgctcacc cgatcgcagg
     1621 gcggcctcgg catcggactc acgctcgtca agcgcctggt cgagatgcac ggaggccgcg
     1681 tgcaggcggc gagcgacggg ctcggccgcg ggtgtgagtt caccgtgacc ctcccgatcg
     1741 cgtccccgag cgacatcggt ggggacgacg agcggcgcga cgtggacgta cgagcggaga
     1801 gccgccgcgt cctggtggtc gaggacgatt cggattccgc ccagagcctc gccatgctgc
     1861 tccagatctg ggggcaccat gtggagatcg cgcacgacgg ccaccaggcg ctcgacgccg
     1921 cgcggagctt cgggcccgag atcgtcctcc tcgacatcgg gatcccgggg ctcgacggct
     1981 atgaagtcgc ggagcggctt cgctcggagc acggcgcgga gctcaagctg atcgcgctca
     2041 ccggatacgg acgcgaggac gaccagcagc gctcgcgcga ggcgggattc gaccggcaca
     2101 tcaccaagcc gctcgagccg cctcgcctcc gcgagatgct cgcgagcttc gacttcggca
     2161 cggagggaga tgccgatcgc cccgatcgac gatcatcggc ccctttgact tgacccggtt
     2221 cagaatcacc ccgacttgcc gataaagccc ctgtccggcg ccaacccaca gggagaagca
     2281 ggttgacttc ggggtatgga ctgattagcg ttcccttcgc tcttctccca tgcgagattg
     2341 ccatccaatg aatcgatacg gccccaggca cttgcgggca ttcgcgctcg ccacggcagc
     2401 ttttgccttc gtcacctgtc tcttcctgtc cggggcctcc gcccccggcc gggccctggc
     2461 cgctccgaca acagcttcga acgtggccga cgcccaggcc ctcgtgaaga agaatcccaa
     2521 ggacccgaac gcgtatttcg cactggggac cgcgtaccgt cgggccggaa agagccagga
     2581 cgccctccag gcctt
//
LOCUS       DASTKC010000095         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1725573, whole genome shotgun sequence.
ACCESSION   DASTKC010000095 DASTKC010000000
VERSION     DASTKC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725573"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFR25_03975"
     CDS             complement(<1..250)
                     /locus_tag="VFR25_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HET9940235.1"
                     /translation="MRSLAEQALDTARKRGADYADVRLLETAREDLQVKNGQVGSVDR
                     SDSVGLGVRVLAKGGWGYAATGFLTKDAVDACAAEAVGI"
     gene            complement(264..1847)
                     /locus_tag="VFR25_03980"
     CDS             complement(264..1847)
                     /locus_tag="VFR25_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940236.1"
                     /translation="MSSFTRSARTLLVVSAILVLPAMAGPARAQVIDAWLNGTPVEPF
                     TGNQSARLKAMGGIKVAIPDDQSRLDPYGYSNNPAGLRESRDSSYVEVPVTYQGHDHT
                     DYGYANSAVLHGAGLRGEFRPGRKWAMAASFDYANVDASRHDECPSPDDCRFIRDFDL
                     PVAPELTPVTTDRTFSAGVESPRISAIYAREFLEGLTLGGRIGYQTESENRRLLIPYD
                     LNVSSARTEFQGGAMYRPHALNSEMTVSGWGQYSKANVTGISESPLNQDEYDWDRPEV
                     AYGGAIHFKNMSWLEAIVDGRHRSFDGEEVARVNWAPQFFMNPLPGDADPDNVFKKHW
                     SAFLSGLRHNEVSTRWLVGLPGRPVHLGAQWGYYREYEWIRANEVVIPSVNELDVKRQ
                     GYRFAGGLSVDLPNKQGVLGAEMQLAREFREDFTNELPDFSRMSSTFNIGGEYRFKPA
                     LPLRAGVELIRLDPNRQDASAPVKGAGLTLGLGYFWNALQWRIDASYVHYHFDSAPDD
                     PADEIGFGDQGYLFIQRAF"
     gene            complement(1894..3096)
                     /locus_tag="VFR25_03985"
     CDS             complement(1894..3096)
                     /locus_tag="VFR25_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940237.1"
                     /translation="MPSGKMLRTGILCAVLAATSLSCGETQPTEPNPVSEITAVRGNE
                     KFLNSPQKESRELLIDGRVTDIEWAITGDASVIRMHNEGGERGGTYYLSVRSLWTVDQ
                     FNAPSGILFLLQWPDRTENRLHMPLVTNADVLADNGDTLIDCTTGNTDLVNPALWTIN
                     RNIQEDEVSIEIFSSDAGAYPKDVWRWGAETTDPANPVNGTEFNNAAQDSAGSLEHPG
                     GSYMEDFFDVGAGPVHDAGNYSYMLANHNSTDFVPLRIASKGTRDTRLNRGKPTNISI
                     WDTVAKDFGPCEIINPIRLDDATAADKTWNPGDYVPSFRLILSTGSQADVVARGAWNM
                     GKWGLEIRRDLIARFPPDSANPILGMPRPDDLQLVPGGHYSVRFTIVDGTTKARSVSD
                     LIPIYLRP"
     gene            complement(3169..>3648)
                     /locus_tag="VFR25_03990"
     CDS             complement(3169..>3648)
                     /locus_tag="VFR25_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HET9940238.1"
                     /translation="DLVLDRVAESGELEAVSATEAAARIPRAAEGRLFPSSWGAEGYS
                     SVWLDPSNDWIYRHLLRASERMAALAREGDRGDVLRGRALRQAGRELLLAQASDWAFI
                     MKTGTLVPYAYRRTEEHLDRFGRLDRALTQGPLEEPEVAAMEARYPIFPDLDPGLFT"
BASE COUNT          612 a         1153 c         1224 g          659 t
ORIGIN      
        1 cgatgccgac cgcctcggcg gcgcatgcgt cgaccgcgtc cttcgtcagg aatccggtgg
       61 ccgcgtaacc ccagcccccc ttggcgagca cgcgcacgcc gaggccgacc gaatcggagc
      121 ggtcgacgga tcctacctgt ccgttcttga cctggaggtc ttcgcgcgcg gtctcgagga
      181 ggcgcacgtc ggcgtagtcg gcgccgcgct tccgcgcggt gtcgagcgcc tgctctgcga
      241 gggagcgcac gctggaggcg aagctagaag gcgcgctgga tgaagaggta gccctgatct
      301 ccgaacccga tctcatcggc cggatcgtcg ggcgccgagt cgaagtggta gtgcacgtag
      361 gaagcgtcga tgcgccactg gagcgcattc cagaagtagc cgagccccaa ggtgagcccc
      421 gcccccttga ccggcgcgct cgcgtcctgg cgattcggat cgaggcgaat cagctccacc
      481 ccggcgcgaa gcggcagcgc aggcttgaag cggtattcac caccgatgtt gaacgtcgag
      541 ctcatccggc tgaagtcggg gagctcgttc gtgaaatcct cgcggaattc ccgcgccagc
      601 tgcatctccg cgccaaggac gccctgcttg ttcgggagat ccacggagag cccgccggcg
      661 aagcgatagc cttggcgctt cacgtcgagc tcgttcacgc tcggaatgac gacttcgttc
      721 gcgcggatcc actcgtactc gcggtagtag ccccactgcg cccccaggtg caccggacga
      781 ccagggagtc ccacgagcca acgggtgctg acttcgttgt ggcggagccc gctcaagaac
      841 gccgaccagt gcttcttgaa gacattgtcc ggatcggcgt cgcccgggag cgggttcatg
      901 aagaactggg gcgcccagtt gacccgcgcg acttcttcac cgtcgaacga gcggtgtcgt
      961 ccgtcgacga tcgcctcgag ccaggacatg ttcttgaagt ggatcgctcc accgtacgcg
     1021 acctcggggc ggtcccagtc gtactcgtcc tgattgagcg gcgactcgct gatgccggtc
     1081 acgttcgcct tggagtactg gccccacccc gagaccgtca tctcggagtt gagggcgtgc
     1141 gggcgataca tggcgccgcc ctggaattcc gtccgcgcgc tcgagacgtt gagatcgtac
     1201 ggaatgagaa ggcgtcggtt ctcgctttcg gtctgatagc cgatccgccc cccgagcgtc
     1261 agcccttcga ggaactcgcg cgcgtagatc gccgaaatcc tcggcgactc gacaccggcg
     1321 ctgaacgtcc ggtcggtcgt gacgggagtc agctccgggg caacgggaag atcgaagtcg
     1381 cggatgaagc ggcagtcatc gggcgaggga cactcgtcgt gacggctcgc gtccacgttc
     1441 gcgtagtcga agctcgcggc catggcccac ttcctgccgg gccggaattc gccgcggagg
     1501 cccgcaccgt gcagcacggc gctgttcgcg tagccgtagt cggtgtgatc gtgcccttga
     1561 tacgtgacgg gcacctcgac gtacgagctg tcgcgggatt cgcggagccc cgcagggttg
     1621 ttggagtagc cgtagggatc gaggcggctc tgatcgtccg gaatggcgac cttgatccct
     1681 cccatcgcct tgagccgggc ggattgattg ccggtgaaag gctcgaccgg cgtgccgttc
     1741 agccaggcgt cgatgacctg ggctcgggcc ggaccggcca tcgcgggaag gacgaggatc
     1801 gcggatacaa cgagaagcgt tcgcgcagag cgcgtgaagg aggacatggc gtactccggg
     1861 actgatacga tgcggggagt ggtcatcggg cgatcagggc cgtaagtaga tcgggatcag
     1921 gtcggagacg ctccgggctt tcgtcgtccc gtccacaatg gtgaaccgca cggagtaatg
     1981 ccctcccggc acgagctgga ggtcgtccgg cctcggcatg cccaggatcg ggttggcgga
     2041 atccgggggg aagcgcgcga ttagatcgcg tcgtatttcg agcccccatt tgcccatgtt
     2101 ccaggcgccg cgggcaacga catccgcctg actcccagtg gaaaggatga ggcggaacga
     2161 gggaacgtaa tcccccgggt tccacgtctt gtcggccgcg gtcgcgtcgt ccaagcggat
     2221 cgggttgatg atttcgcaag ggccgaaatc cttggccacg gtatcccaga tcgagatgtt
     2281 ggttggcttg cctcgattga ggcgcgtgtc acgcgtgccc ttgctcgcga tgcgaagtgg
     2341 gacgaaatcg gtcgagttgt gattggccag catgtacgag tagttacccg cgtcatgaac
     2401 cggccccgcg cccacgtcaa agaagtcctc catgtaggag ccgccggggt gctcgaggga
     2461 accggcgctg tcctgggcgg cattattgaa ctccgtaccg ttcaccggat tcgccgggtc
     2521 ggtcgtctcc gcgccccagc gccaaacatc cttgggatag gcgccggcat cgctcgagaa
     2581 gatctcgatc gagacttcgt cctcctgaat gttccggttg atcgtccaca gggccgggtt
     2641 cacgaggtcc gtgttccccg tggtgcagtc gatgagcgtg tcgccgttgt cggcaaggac
     2701 atcggcattc gtcacgagcg gcatgtgaag gcggttctcc gtccgatccg gccactggag
     2761 cagaaagagg atgccggagg gtgcgttgaa ctggtccacg gtccagaggc tccggacgga
     2821 gaggtagtac gtccccccgc gttcaccccc ctcgttgtgc atgcggatca ccgacgcatc
     2881 gcctgtgatg gcccactcga tgtccgtcac ccggccgtcg atcaggagct cgcgcgactc
     2941 cttctgaggc gaattcagaa atttctcgtt gccccgaacc gctgtgatct cgctgacggg
     3001 gttgggttcg gtgggttgag tttcgccgca ggagaggctc gtggcggcga gcacggcaca
     3061 aagaatccct gttcggagca tcttcccaga tggcatctat cgtcctcgga tcattcggac
     3121 tgtagggggg gaatcgggcg gtacgttacc gatcgggtcg cttttctgtc aagtgaaaag
     3181 ccccggatcg aggtcgggga aaatcgggta ccttgcttcc attgcagcca cttccggctc
     3241 ctcgaggggc ccctgggtga gcgcgcgatc caggcgtccg aagcggtcca ggtgctcctc
     3301 ggtgcgtcga tacgcgtacg ggaccagggt cccggtcttc atgatgaacg cccagtcgct
     3361 cgcctgggcc aggaggagct cgcggccggc ctggcggagg gcccggccgc gcagaacgtc
     3421 ccctcggtcc ccttcccggg cgagcgccgc cattctctcc gacgcgcgga gcagatggcg
     3481 gtagatccaa tcgttcgaag gatccaacca gacactcgag tacccttccg caccccagct
     3541 cgacggaaag agccgcccct ccgccgcgcg cgggatgcga gccgcggcct cggtcgcgga
     3601 caccgcctcg agttccccgc tttcggcgac ccgatcgagg acgagatc
//
LOCUS       DASTKC010000096        18984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_781083, whole genome shotgun sequence.
ACCESSION   DASTKC010000096 DASTKC010000000
VERSION     DASTKC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18984
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781083"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFR25_03995"
     CDS             complement(<1..181)
                     /locus_tag="VFR25_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9940239.1"
                     /translation="METIFERMRNDHRRVLDEVAAMEEAMTSGKDATQAVSRLLALLA
                     RQFGSHMAAEDEVLYP"
     gene            369..1076
                     /locus_tag="VFR25_04000"
     CDS             369..1076
                     /locus_tag="VFR25_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9940240.1"
                     /translation="MAITQPERVLQAVRMTPLFRGLSPDDQGRIASVATIKDFKRGDR
                     LWSEGDPSESLTVIVRGRVKIVKEGESSDVILEIFGEGEPVGAIAVYNYMPYPAGAIA
                     MEPVTLLVVPRRDYFELLDRHPDFARACIRELTKLVVSLTRKLEEMRGQKVEVRIAQL
                     FLSLAERMGIESKGGIEVPLHLSRQEIADLTGTTVETAIRTLSRWGHEKIVITGEKSF
                     HIPSRERLERVAEGREK"
     gene            complement(1081..1482)
                     /locus_tag="VFR25_04005"
     CDS             complement(1081..1482)
                     /locus_tag="VFR25_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940241.1"
                     /translation="MLIRWGAPEVTPTDLDPTMMRVVHAFEDGLEPFELEIGPGSLDI
                     APLERGKPRRCRLKLFRPREGKERLCAFFYKRSNLAWSRDRFSYGGVEFRPEQATDED
                     VASWRAWLLSGFDPERRPPGWRRAFLFDIPE"
     gene            complement(1476..2708)
                     /locus_tag="VFR25_04010"
     CDS             complement(1476..2708)
                     /locus_tag="VFR25_04010"
                     /inference="COORDINATES: protein motif:HMM:NF045502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9940242.1"
                     /translation="MRPAPLNPCELEIDLFCRGIRIDSSCALPEDGRGLARTRAGLGS
                     GLELTLPGPLKDVWVNVPVEEDFARASPYRLVKSRGSYRILDERSEHRYPVRVPPEPA
                     WYGRRTSAGTPMRRVGVLQGTYLGVYISNSCLFWYSNPSMHCRFCTTGANVGVNEVAL
                     KGLEDVVEVCRAAKEESGVTFFHFNSGYSKRCHEEAMPFVKAVKERVGGLVGLQLMPS
                     RDLTVYDRLIDLGVDHFSFCYEFMNPEYFARYLPGKEAVLGQRAFFDALEYTARKLGK
                     GAVSGEIIAGVEPIEDTLRAIDYITSLGAFPTVCIFRPVIGSDMERHPSPRYEDMRTV
                     FEHVYEACRRNRIPMGLAPNLEVSLVCQPDDTRYLAPSGFATWMYEAWLSAMRRLAQP
                     TFRKRMAPRIPARSTGPC"
     gene            complement(2716..3213)
                     /locus_tag="VFR25_04015"
     CDS             complement(2716..3213)
                     /locus_tag="VFR25_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940243.1"
                     /translation="MPDIQALLPDPGACTVNARFHPVVAATILALAMAATPVPANTQS
                     PPPASKPPKVAVVRGELLDLSCWLARGLSGPLHQDCAKRCIASGVPMGIITADSTLWL
                     LTQEHGRSMAPGSYAGSPDPFVQCKDWPSYTVEVKGLASTRNGVHVVEVARATLISKP
                     AATTP"
     gene            complement(3294..4598)
                     /locus_tag="VFR25_04020"
     CDS             complement(3294..4598)
                     /locus_tag="VFR25_04020"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9940244.1"
                     /translation="MPTDVKQGTAETANLTYNGTSLSLPVIKGTEDEAAVDIEKLRSA
                     TGLITLDPGYGNTGACRSAITFIDGEKGILRYRGYPIEELAARSTFLEVAYLLIHGEL
                     PTRTELDGFTHEVTIHTMLHQDFGRFFDALPKDAHPMPVCAAAVGALATFYQKPESEL
                     QIHETIIRLIAKMPTIAASSYKHSIGQPFIYPQNRLDYTSNFLNMMFSVPSDTYEVDP
                     VLARTIDLLLILHADHEQNCSTSTVRMVGSSMANLYASIAAGISALWGPLHGGANQQV
                     IEMLERIAAEEGSADKFLTKAKDKNDTARLMGFGHRVYKSYDPRAAILKKACSQVIER
                     VGQSSQLLEIAMHLEEIALKDEYFVSRKLYPNVDFYSGVIYQALGIPVNMFTVMFTLG
                     RLPGWIAQWLEMRRDKDFRIGRPRQIYVGSPQRPYVPIGNRA"
     gene            complement(4660..5388)
                     /locus_tag="VFR25_04025"
     CDS             complement(4660..5388)
                     /locus_tag="VFR25_04025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET9940245.1"
                     /translation="MASDRRPSVLAIQHVEPETTGLLGEALAEGGAQLTTVRVFAGEP
                     VPADIAPHDALVVMGGPMGVYEADQHPHLADEVELIRRAVENEVPVLGVCLGSQLLAA
                     ALGSSVRPAHKELGWLPVTLTLEGTADPLFAGLPRTLTVFHWHGDAFDPPPRSVALAS
                     SDLTPCQAFRHGAHVGLLFHMEMSESMIRALVEQDLASGYADALTARRALEEMQGRLP
                     AMRAFARKVFGRVSDAIAGKPLRA"
     gene            5492..6142
                     /gene="msrA"
                     /locus_tag="VFR25_04030"
     CDS             5492..6142
                     /gene="msrA"
                     /locus_tag="VFR25_04030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9940246.1"
                     /translation="MRFLAAILALLVLVAPCPVRSAPSTPAASSMAKPEVKHATFAGG
                     CFWCMETAYEGRPGVVAVISGFAGGKEANPTYDEVSAGRTGHMESVEVVYHPSEISYE
                     RLLEIYWHNIDPTQGDGQFCDRGAQYRSAIFVRDDNERKQAERSKSAIEKAKTLHAPI
                     VTRILPFTGFWPAEEYHQDYFRKNPDDYHRYREGCGRDRRLRDLWGAKAGEDPAVH"
     gene            6849..7331
                     /locus_tag="VFR25_04035"
     CDS             6849..7331
                     /locus_tag="VFR25_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET9940247.1"
                     /translation="MKTPTRMAFTLWAMVGALAGAMTWGVGPARAGDAVSKSSQPYIA
                     PIVPTFEKSEHRGRIGVGEEIRDHGYSLRQDLIKKRTIGDIIDRSKGHDGTRGGWIGG
                     GTEGGQPPETIDAPFETQPISTSYRPAGGPPPAPEPSGFVLAGIGVAGLILSGRRRKR
                     "
     gene            7408..8265
                     /locus_tag="VFR25_04040"
     CDS             7408..8265
                     /locus_tag="VFR25_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940248.1"
                     /translation="MSFADPSSFRLPRLRFLPVEQLVPHEQQDDRRTGALRSRLKDSG
                     VLKNPPVVTSAEAIGDPRYVVLDGANRVATLQAAGFPHAVVQIVDYDDPGVRLTTWHH
                     ALTHDLGTEIEKAIASIPGLTLREMDLTHARALLARREAIAFLVRAGACVLSLHGGQD
                     LHERNALLNAVVDRYRSRVPYYRVTRDSLEDARARYPDVESLLVFPHFHPEEILEVAL
                     SGARLPAGITRHVIAWRALRLNLPLTLMADPDRTLDEKNRWLEQWIADRTATSSVRFY
                     EESTVLFDE"
     gene            8262..9233
                     /locus_tag="VFR25_04045"
     CDS             8262..9233
                     /locus_tag="VFR25_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9940249.1"
                     /translation="MTAPRVKALDPPRQEEIRAARERIRGLAVRTPLLRLPLEDAEIY
                     LKLETLQPIGSFKIRGAGNAMRSAPRDLLARGVYTASAGNMAQGVAWCAREMGIRCTV
                     VVPDDAPRAKLDAIERLGARALLVPYADWWTVLEEHRYPGLEGLFVHPVAAPGVIAGN
                     GTIGLEILEDLPDVDAVLVPFGGGGLSSGIASAIRAEKPGVPVYACEIETAAPLAASW
                     AAGVPTSVDRIPSFVDGIGGKGVLPEMWALIRSVIEGPIVVSLEETAAAVRLLVARAR
                     IVAEGAGAVPVAAALAGRAGQGRLACVVSGGNIDPQKLATILMGGLP"
     gene            9338..9835
                     /locus_tag="VFR25_04050"
     CDS             9338..9835
                     /locus_tag="VFR25_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940250.1"
                     /translation="MHDLNPQELRRAILTHHLARLTWVAFIITFVIARSIALLRTDFH
                     IMVGETHLHHLNVGIILLAAMGGYLLFRRPGGHELRSAAVLYGVALGLTFDEFGMWLH
                     LEDEYWQRASYDAVGVIGAFLGLLAAGPTLRRVRRKYWATVAGAAIVAVLFGLLVLKP
                     FFSPG"
     gene            complement(9854..11377)
                     /locus_tag="VFR25_04055"
     CDS             complement(9854..11377)
                     /locus_tag="VFR25_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9940251.1"
                     /translation="MGARRGSALALILAGMAAWVLAAGPARAQVPTLTTVRVASGLNT
                     PLYVTAPPGDTTRIFVVERRGSDQRGRIKIVRNGAVLPTPFLTTDVLSSSTEQGLLGL
                     AFAPNYAQTGRFYVYYTDAAGSTQIVRYQVSADANVANPNGTVILSIPHPYANHYGGW
                     IGFGPDGYLYIAMGDGGDEGDPGDRAQDLNTFFGKILRIDVSGSGYTIPPGNPYAGAT
                     PGLDEIWCFGLRNPWRDSFDRQTHDFIIADVGQSSSEEVDFFPAGTGAGANLGWRCRE
                     GSLPYATSTTTPCGSCSNPSCPFIAPATEYGHTGGKCSITGGYVYRGSKIPALQGSYF
                     YADYCSKQIWSGTFQQGSLTNIVDRTAELAPVGGVGQYSIGFVTSFGEDARGELYICD
                     QDGDVFRIVTKASVVGVDTGGPPASVQLRLMGRLPFYGQLVLEVDLPAPADASVDVFD
                     VRGRKVRTLHRGTMPAGRTPLGWDGRLDDGAPAPSGVYWVRLQAAGVVSSARTVLLR"
     gene            complement(11377..12126)
                     /locus_tag="VFR25_04060"
     CDS             complement(11377..12126)
                     /locus_tag="VFR25_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940252.1"
                     /translation="MDRIQCRWRVSALAVLLPIAALAALGRPMQARATPPGYFVDQAE
                     AEGYGDRPFRLDETAYAFSVSVDRAASDTLSARLLAIAESTYPERDPLVVNALDHRWE
                     MGADTAHAVVWWSRGVGPNRIPQAATWRAVRYYADLCAHYRRNLYLNTGTRAPMKSEL
                     TYWATIDHKDRFGLEGSQFNDVYVANMNLFWGIDDGAFDMITTAHRTVVIDPSGEVLQ
                     VVGDAPGDESVMISGWRDRWRESANSARRPH"
     gene            12326..12799
                     /locus_tag="VFR25_04065"
     CDS             12326..12799
                     /locus_tag="VFR25_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9940253.1"
                     /translation="MASTKPIPDGFTTVTPSLCLKDCNKAIDFYKKALGAQELMRMPG
                     PDGKIMHAEIKVGNSHVMMNDEIMGSRSAETLGASPVNFYLYVEDADGAHKKAIAAGC
                     KEIMPVADMFWGDRMGAVSDPYGYKWNFSTHIKDLSPEEIKKGQDEWMKQMAGSR"
     gene            complement(12861..15155)
                     /locus_tag="VFR25_04070"
     CDS             complement(12861..15155)
                     /locus_tag="VFR25_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9940254.1"
                     /translation="MIRSASIRGGLIAAALLASSLTPSAAVATDDGVWSFLSFPPPAA
                     RVQHAAIYDPVRNRMLVFGGLAGSSLKNDVWELSLAGTPTWKLLAVAGTPPSARRGSS
                     AIYDPVRDRMLVYGGAAPGALGDVWALSLGASPAWTQLSPTGSPPPSRSGHSAIYDPV
                     RDRMVVFGGQTGLTYRNDAWALALAGTPAWSNVAPSGGPPDLRARHSAIYDSVRDRLV
                     VFGGVNNTGLLGDLWSLSFAGTATWTLVTPTGTPPPPRAGSKAIYDVTRDRMVVFGGD
                     DGSGEPPGTTWALPFVSSAWTQLTPSGNIPAGRYDHVAVLDTAGDRMLVFGGLADQPT
                     NELLSLSLSGGTAWATLAPLGGPAPPRTDHAAAYDAARDRMIVFGGQAANGLLQDAWA
                     LVLGTQTWVNLNPSGTKPDARDGHLMVYDSTHDRMLVFGGSDVGNHQLNDVWSLTLGA
                     SPAWTKLTPVGTPPPERFGACGVYDPAGDRLVIFGGLGDLLYSDVWALSLSGTPTWSQ
                     LTPSGTPPAARFLAASTRNPVTNSMVLFGGTDSALFNDVWSLSLSGAPAWTSISASGT
                     APSPRYQPTAIYDQSRDRLVVFGGFDGTTFCGDTWGLSLTGTPTWTALQPTGLSPSSR
                     SGHVAIYDPVRQSMLVYGGLDGAVQNDTYELLFGQVTAVAEAPAGSAPAAMTLHAAYP
                     NPFRSSAAIDFDLARPREGRIAVYDVHGRLVRELRRGLLPQGSSSLRWDGTDGRGVLV
                     AAGAYFYRIESEGASLTRKVVLLH"
     gene            complement(15152..15928)
                     /locus_tag="VFR25_04075"
     CDS             complement(15152..15928)
                     /locus_tag="VFR25_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9940255.1"
                     /translation="MRIPVPPSVVVPVVSAALVLSLAGHALAQQPPAWDWPDKPKNLK
                     VLPKTTSKEELRGAMMAFTRSLGVRCNYCHEGEEGKPLSSFDFASDKNPKKDIARDML
                     RMTGDVRADLKKMKLQGTHRVTVGCITCHHGRPTPATLVEELGWTYETSGIDSTLASY
                     QALRTRFYGRNAYDFGDGSLIELGTSLSDKGKFEDAVRVMKLNVEQNPNSSRALDGLG
                     GVYLAAGQKDNAAEAFHKALQLDPKDRLAEEGLKRLEGMK"
     gene            16109..16921
                     /locus_tag="VFR25_04080"
     CDS             16109..16921
                     /locus_tag="VFR25_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9940256.1"
                     /translation="MNPYILGHSDHETRRLILQHQIYGPITRRFFEAAGIGRGMKVLD
                     IGSGAGDVALLVADLVGPSGRVVGVDMNAEILNTARERAMVAGWSNVEFLAGDFAAVV
                     TDKDFDAVVGRWVLMHVPDPVTLVRNAAGRLRVGGIVAFHENDFTYPPTTFPPTPVDI
                     QVGRWLVPPPGGRGPEMQMGTKLYTTYLDAGLPAPQFRLEAPLGGGPEWPGFEYVAET
                     ARSLLPMLEKITGVKPEEIQVDTLGARLKDDAMKHRAVQMLPLMFGAWTRKV"
     gene            16970..18199
                     /gene="purT"
                     /locus_tag="VFR25_04085"
     CDS             16970..18199
                     /gene="purT"
                     /locus_tag="VFR25_04085"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HET9940257.1"
                     /translation="MRLGTPLAPGATKVMLLGAGELGKEVLIALQRLGVETIAVDRYP
                     NAPGHQVAHLAHVIDMTDPAAIRAVVEAERPHLVVPEIEAIATEELVRIEEDGLATVI
                     PTARATRLTMNREGIRRLVAETLRLPTSRYEFAESEAELAAAASRIGFPCVVKPVMSS
                     SGKGQSVVSSPGDVARAWEYAESAGRVKHGRVIVEEMIQFEYEITLLTVRSAGENGAS
                     RTSFCEPIGHVQVRGDYVESWQPQPMGDVALARARDIAKRVTDDLGGRGVFGVELFVR
                     GDQVWFSEVSPRPHDTGMVTMATQVQSEFELHARAVLGLPVSTALRSPGASAVIYGGL
                     EARGIAFEGVDEALRVPQTDLRLFGKPESFTRRRMGVALAWGDDVNEARVRAKLCAGK
                     VKPVEAGSPASEAPVPA"
     gene            complement(18207..>18984)
                     /locus_tag="VFR25_04090"
     CDS             complement(18207..>18984)
                     /locus_tag="VFR25_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9940258.1"
                     /translation="GLCTILAPVLLPRLPGWMDPLRTETPDEVNTMHSGITRIGTLLA
                     AAAFLAAGTTGCASLNKTQKGAIVGAAGGAAVGGVVGKYAGSTTKGAIIGAVVGGAAG
                     AVIGHRMDEKAQELEAKLPGAKVDRVGEGILVTFPSGLLFDFDSDAVRGEASHNLNTL
                     AESLEKYDNSDLLIVGHTDDVGTESYNQGLSERRADAAANYLRARGVRRSIRTVGRGE
                     DEPIVSNATDEGRSKNRRVEVAIYASEEFRDQAASQAASR"
BASE COUNT         3114 a         6352 c         6445 g         3073 t
ORIGIN      
        1 cggggtagag cacttcgtcc tccgccgcca tatggctccc gaactgccgc gcgaggagcg
       61 ccaggaggcg ggacacggcc tgcgtcgcgt ccttgccgct ggtcatcgcc tcctccatgg
      121 ccgcgacctc gtcgagcacc cggcggtggt cgtttctcat tcgctcgaag atcgtttcca
      181 tcgttcctcc cgtgtggatc acgagatgaa ttgtcggaga ttggaggcga agcgcccatg
      241 accgcaatca tggcgcgttg cgatttccgg gatcccccag gaggcgccaa cccgagccgg
      301 gaccctacgt tgagggtgag gggcctggaa cggtatgatg cccgtcatat gctccctcga
      361 gagtccccat ggccataacg cagcccgagc gcgtgctcca ggccgtgcgg atgacgccgc
      421 tctttcgcgg cctgtcgccc gacgatcagg gacgcatcgc atccgtcgcg acgatcaagg
      481 acttcaagcg cggcgaccgg ctctggagcg agggcgatcc gtccgaatcc ctgaccgtga
      541 tcgtgcgcgg ccgggtgaag atcgtgaagg agggcgagtc gtccgacgtg atcctcgaga
      601 tcttcgggga gggcgagccg gtcggagcga tcgcggtcta caactacatg ccctaccccg
      661 ccggcgcgat cgccatggag ccggtgacgc tcctcgtggt ccctcgtcgc gattacttcg
      721 agctcctcga ccgccatccc gactttgcgc gcgcctgcat ccgcgagctg accaagctgg
      781 tcgtctcgct cacccgaaag ctcgaggaga tgcgcgggca gaaggtcgag gtgcggatcg
      841 cccagctctt cctgagcctg gccgagcgga tggggatcga gtcgaaaggg ggcatcgagg
      901 tgccgctcca tctgtcgcgg caggagatcg cggacctgac cggaacgacg gtcgagaccg
      961 cgatccgcac gctctcgcgc tgggggcacg agaagatcgt catcacaggc gaaaagagct
     1021 tccacattcc ttcgcgagag cgcctcgagc gcgtggccga gggtcgcgag aagtagcgga
     1081 ctactccgga atgtcgaaga ggaaggcgcg gcgccatccc ggcgggcggc gctccggatc
     1141 gaagcccgag agaagccacg cgcgccagct cgcaacgtcc tcgtccgtcg cctgctccgg
     1201 gcggaactcc acgccgccat agctgaaccg gtcccggctc caggcgagat tgctccgctt
     1261 gtagaagaac gcgcagagcc gctccttccc ctcgcgaggc cggaacagct tgagccggca
     1321 tcgccgcggc ttgccccgtt cgagcggagc gatatcaaga ctgccaggac cgatctcgag
     1381 ctcgaagggc tcgagcccat cctcgaacgc gtggaccacg cgcatcatcg tcggatccag
     1441 atcggtcggc gtgacctcgg gcgcccccca gcggatcagc atggcccggt agagcgcgcc
     1501 ggaatgcgtg gagccatgcg cttccggaag gtcggctgtg cgagtcgacg catggcggag
     1561 agccaagcct cgtacatcca ggtcgcgaag ccggagggtg cgagatatcg cgtgtcgtcg
     1621 ggctggcaga ccaggctcac ctccagattg ggcgcgagcc ccatggggat ccggtttcgc
     1681 ctgcaagcct cgtacacgtg ctcgaagacg gttcgcatgt cttcgtatcg cggagacgga
     1741 tggcgctcca tgtcggagcc gatcaccggg cggaagatgc agaccgtggg gaacgcgccc
     1801 aggctcgtga tgtagtcgat cgcacgaagg gtgtcctcga tcggctcgac gcccgcgatg
     1861 atctcccccg acaccgcccc cttccccagc ttccgcgccg tgtactcgag cgcgtcgaag
     1921 aaggcgcgct gcccgagcac cgcctcctta cccgggagat agcgggcaaa gtactccgga
     1981 ttcatgaact catagcagaa ggagaagtgg tccacgccga gatcgatgag ccggtcgtag
     2041 acggtgagat cccggctcgg catgagctgg aggccgacca ggccgcccac gcgctccttc
     2101 accgctttca cgaacggcat cgcttcctcg tgacaccgct tcgagtagcc gctgttgaag
     2161 tggaagaacg tgacgccgct ctcttccttc gccgcgcggc agacctcgac gacgtcctcg
     2221 agtcccttga gcgcgacctc gttcaccccg acgttggcgc ccgtggtgca gaagcggcaa
     2281 tgcatcgatg gattcgagta ccagaagaga cacgagttcg agatgtagac gccgaggtac
     2341 gtcccctgga gcacgccgac gcgccgcatc ggcgttcccg ccgacgtgcg gcgcccgtac
     2401 caggcaggtt ccggcgggac tcgcacggga tagcggtgct cggagcgctc gtccaggatc
     2461 cggtagctcc cgcgggactt cacgagtcga tagggggatg cgcgcgcgaa gtcctcctcg
     2521 accggcacgt tcacccagac gtccttgagc ggccccggga gggtgagctc gagtccggat
     2581 cctaggccgg cccgcgtccg cgcgaggccg cggccgtcct cggggagagc gcacgaggaa
     2641 tcgatgcgaa tgccccggca gaagagatcg atctcgagct cgcaggggtt gagcggagcg
     2701 ggacgcatgc gcggctcagg gggtcgtggc ggccggcttc gagatgagtg tcgcgcgcgc
     2761 cacctcgacc acgtggacgc cgttccgggt cgatgcgagc cccttcacct cgaccgtata
     2821 ggagggccag tccttgcact gaacgaaggg gtcgggcgat cccgcataac tccccggtgc
     2881 catcgagcgg ccgtgctcct gcgtgaggag ccagagggtg ctgtccgccg tgatgatccc
     2941 catgggaacg ccggacgcga tgcaccgctt cgcacagtcc tggtggaggg ggcccgagag
     3001 tccccgggcg agccagcagc tcagatcgag gagctcgccg cgaacgaccg cgaccttcgg
     3061 gggcttcgac gcgggcggcg gactctgagt gttggccggc accggggtgg cggccatggc
     3121 aagcgcaagg atcgtcgcgg ccactacggg gtgaaatctg gcgttcacgg tgcacgctcc
     3181 gggatcggga agcagcgcct ggatgtccgg caccgatgag gctaccaccc gaacacgaag
     3241 aagccgcatg ggaccaaagt cccatgcggc cggagcctag cggcgcggtg cgatcaggcg
     3301 cgattgccga tgggcacgta ggggcgctgg ggcgagccga catagatctg gcgcggacgt
     3361 ccgatgcgga aatccttgtc tcggcgcatc tcgagccact gcgcgatcca gcccggcagg
     3421 cggcccagag tgaacatcac cgtgaacatg ttcaccggga tgccgagcgc ctgatagatc
     3481 acgccggagt agaagtcgac gttggggtag agcttccggc tcacgaagta ctcgtccttc
     3541 agcgcgatct cctccaggtg catggcgatc tcgaggagct ggctcgactg gcccacgcgc
     3601 tcgatgacct ggctgcaagc cttcttcaaa atggccgccc gcggatcgta ggacttgtac
     3661 acgcggtggc cgaatcccat gagccgcgcg gtgtcgttct tgtccttggc cttcgtgagg
     3721 aacttgtcgg cgctcccctc ctccgccgcg atccgctcga gcatctcgat cacctgctgg
     3781 ttggcgccgc cgtgaagcgg tccccagagc gcgctgatcc cagccgcgat gctcgcgtag
     3841 agattcgcca tcgagctccc caccatgcgg accgtgctgg tcgagcagtt ctgctcgtgg
     3901 tcggcgtgga ggatcaagag gagatcgatc gtccgcgcga gcacgggatc gacctcgtac
     3961 gtgtcgctgg gaaccgagaa catcatgttg aggaagttcg acgtgtagtc gaggcggttc
     4021 tgcggataga tgaacggctg cccgatcgag tgcttgtagg agctggccgc gatcgtcggc
     4081 atcttcgcga tcaggcggat gatcgtctcg tggatctgga gctcgctctc cggcttctga
     4141 tagaaggtcg cgagcgcgcc caccgcggcg gcgcagaccg gcatcggatg cgcatccttg
     4201 ggaagcgcat cgaagaagcg cccgaagtcc tggtggagca tcgtgtggat cgtgacttcg
     4261 tgcgtgaatc cgtccagctc ggtccgggtt ggaagctcgc cgtggatcag gaggtaggcc
     4321 acctcgagga acgtgctccg cgcggcgagc tcctcgatgg gatacccgcg gtagcgcagg
     4381 attcccttct ctccgtcgat gaacgtgatc gcgctccggc aggcgccggt gttgccgtag
     4441 cccggatcca gcgtgatcag cccggtggcc gagcgaagct tctcgatgtc gaccgcggcc
     4501 tcgtcctccg tgcccttgat gaccggaagc gagagtgagg ttccgttgta ggtcaggttg
     4561 gcggtctcgg cagttccctg tttgacgtcg gtcggcatca gtcccctcct cacaggatca
     4621 tcgatcgcgt gcctagagta taggacgagc gcccgtgcgt cacgcgcgga gaggcttccc
     4681 cgcgatcgcg tcgctcacgc ggccgaagac cttgcgggcg aaggcgcgca tggcgggcag
     4741 gcggccttgc atctcctcga gggcgcggcg cgcggtcaag gcgtcggcgt agcccgaagc
     4801 gagatcctgc tccacgaggg cgcgaatcat ggactcgctc atctccatgt ggaacaggag
     4861 cccgacgtgc gcgccgtggc ggaacgcctg gcatggggtg aggtcggagg acgcgagcgc
     4921 gacggacctc ggcggtggat cgaacgcatc gccgtgccag tgaaagaccg tgagggtccg
     4981 tggcaagccg gcaaagagcg gatcggcggt cccttcgagc gtgagcgtca cggggagcca
     5041 cccaagctcc ttgtgcgcgg gacgcacgct cgagccgagc gctgccgcga ggagctggct
     5101 cccgagacag actccgagaa cggggacctc gttctcgacc gcgcggcgga tcaattcgac
     5161 ttcatcggcg agatggggat gctggtccgc ctcgtagacg cccatggggc cgcccatcac
     5221 gacgagagcg tcatggggcg cgatgtcggc ggggaccggc tcccctgcga acacgcgcac
     5281 cgtcgtgagc tgcgcccctc cttccgccag ggcctcgccg agcagtccgg tcgtctcggg
     5341 ctcgacgtgc tggatggcca gcacggacgg ccgccggtcc gaagccatca caagctcccc
     5401 tccgtcacga tgcctcccat tccgcgtatg ctcgttcgag tgggcgatcg cgcccgcgcg
     5461 tcctcagata ccacgaaccg gaggccgaag catgagattc ctcgccgcta tcctcgccct
     5521 cttagtcctg gtcgctccgt gccccgtccg gtccgcgcca tccaccccgg ccgcgagctc
     5581 catggccaag cccgaagtga agcatgccac gttcgcggga gggtgcttct ggtgcatgga
     5641 aactgcctac gagggaaggc ccggagtcgt cgccgtgatc tccggattcg ccggcggcaa
     5701 ggaagcgaat ccgacctacg acgaggtgtc cgccggtcga acggggcaca tggaatcggt
     5761 ggaggtcgtc taccacccga gcgagatcag ctacgagcgc ctcctcgaga tctactggca
     5821 caacatcgat cccacacagg gagacgggca gttctgcgac cgaggcgctc aatatcgctc
     5881 ggccattttt gtccgcgacg acaacgagcg caagcaggcc gagcgctcga agagcgcgat
     5941 cgagaaggcc aagacactgc acgcgcccat cgtgacgcgc atccttccgt tcaccggctt
     6001 ctggcccgcc gaggaatatc accaggacta cttccggaag aacccggatg attatcaccg
     6061 ctaccgggaa gggtgcggac gtgaccgcag acttcgagat ttgtggggag ccaaagcggg
     6121 ggaggatccc gccgttcact agtggcggaa tccctggtcg aatagcaact tacgccggac
     6181 actcctgtcg gaaccctgat gtcacagaca tttttgtctc agagtaaata tttgtttact
     6241 catgaataac ggggctggta tactcctttg tacgtcggcg gaccaaagag ttagcacgcg
     6301 gtcccgaatg catcgttcat tgaaatcgta acctgccgac gacgcaacgt tccctggagc
     6361 ctcgcatgcg tatcctcgtc ccagcagtcg cactcgcgtt tctggttttg tcgtacgcct
     6421 ccgagtgcag cgcttcgagc ggtcgccctt cacctcgccc cgatggatcc aagacgcaag
     6481 tcatggagct tcacaaaggc gatatgtcga agccggccaa gcagactccg ccaccggacc
     6541 tgtcgccatc cgatctgaca acgacagaaa aagtttccgg tgttccggac gctccggagc
     6601 cgggcacgct cgtgctcgtc ctgtccgcgg gtgcgatcgc gatcttccag cgccgccgtt
     6661 ccgcgtaaga caagtcaaag cgctgtacca cgctagccag aaggcacctc gcccttctgg
     6721 ctttcgcgcg tttggctcgc cccccattcc tcctcaacgt ccaacccttg gcactcaagc
     6781 tgcattagct gccagtgcct ggggttctga gcgaatccga accccatgga cacagggaag
     6841 gggggtcaat gaagacaccg acaaggatgg cgttcacgct gtgggcgatg gtcggcgccc
     6901 tggccggagc aatgacgtgg ggcgtgggac ccgcgcgcgc cggcgacgcc gtttccaagt
     6961 cgtcccagcc ctacatcgcc ccgatcgtcc cgacgttcga gaagagcgag catcgcggaa
     7021 ggatcggggt gggtgaggag attcgggatc acggttactc gttgcgccag gacctgatca
     7081 agaagagaac gatcggggac atcatcgacc gctcgaaggg ccatgatggc actcggggcg
     7141 ggtggatcgg cggcggcacc gaagggggtc agccgcccga gacgatcgat gcacccttcg
     7201 agacgcagcc catctcgacg tcgtatcgac ccgcgggcgg tccgccacct gctccggagc
     7261 cgagcggatt cgtcctggca ggcatcggag tggcagggtt gatcctctcc gggaggcgaa
     7321 ggaagcgctg atcgacccat cgcgaacgcg agtggtccgg gctcctcggc gaaggccggg
     7381 gagcccgctg tgctaggctc ccgagccatg tccttcgccg atccatcgtc gttccgtctg
     7441 ccgcgtctgc ggtttcttcc ggtcgagcag ctcgtgccgc acgagcagca ggacgaccgg
     7501 cggacggggg cgctcaggtc ccggctcaag gactccggcg ttctcaagaa tccgccggtc
     7561 gtgacctcgg ccgaggcgat cggcgatccc cgatacgtcg ttctggacgg agccaatcgc
     7621 gtggccacgc tccaagcggc cggctttccc cacgcggtcg tgcagatcgt ggactacgac
     7681 gatcccggcg tacggctcac gacctggcac cacgcgctca cccacgatct cgggacggag
     7741 atcgagaagg ccatcgcgtc gatccccggg ctcaccctcc gcgagatgga tctgacccat
     7801 gcgcgggcgc tcctcgcgcg gcgcgaagcc atcgcgttcc tggtccgggc cggcgcgtgc
     7861 gtgctctccc tgcacggtgg acaggacctc cacgagcgga acgcgctcct gaacgccgtg
     7921 gtcgatcgct accgctcgcg cgtgccctac tatcgcgtga cccgggattc gctcgaggac
     7981 gcgcgcgcgc gttatcccga cgtggaatcg ctgctcgtgt tcccccactt ccatcccgag
     8041 gagatcctcg aggtggcgct cagcggtgcc cgcctccccg ctggcatcac gcgccacgtg
     8101 atcgcgtggc gggctctccg gctcaacctg cccctgacgt tgatggcgga cccggaccgc
     8161 acgctggacg agaagaaccg atggctcgag cagtggatcg cggaccgcac cgccacgagc
     8221 tccgtgcgct tctacgagga gagcacggtg ctcttcgacg agtgaccgcc cctcgtgtga
     8281 aagcgctcga cccgccgcgc caggaggaga tccgcgccgc gcgcgagcgg attcgcggac
     8341 tggccgtccg cactcccctc ctccgccttc ctctcgagga cgccgagatc tacctgaagc
     8401 tggagacgct ccagcccatc ggctcgttca agattcgggg cgcgggaaac gcgatgcgga
     8461 gcgcgccccg ggaccttctc gctcggggcg tctacaccgc gagcgcaggg aacatggcgc
     8521 agggcgtggc gtggtgcgcg cgcgagatgg gcattcggtg cacggtggtc gtgcccgacg
     8581 acgcgccgcg agccaagctc gatgcgatcg agcggctcgg tgcgcgcgcg cttctcgttc
     8641 cctacgcgga ctggtggacc gtcctcgaag agcaccgcta tccgggtctc gagggcctct
     8701 tcgtgcatcc cgtcgcggcc ccgggagtga tcgccggcaa cggcacgatc ggcctcgaga
     8761 tcctcgagga tctccccgac gtggacgcgg tcctcgtccc gttcggaggg ggcgggctga
     8821 gcagcggcat cgcatccgcg attcgcgccg agaagccggg cgtgccggtc tacgcgtgcg
     8881 agatcgagac ggcggctccg ctcgcggcgt cgtgggcggc gggagtaccc acctccgtcg
     8941 accggatccc gagcttcgtc gacgggatcg gcgggaaggg cgtcctaccc gagatgtggg
     9001 ccctcatccg gagcgtcatc gaaggaccca tcgtcgtgtc gctcgaggag acggcggccg
     9061 cggtgcggct cctcgtggca cgcgcccgta tcgtggccga aggggcaggc gcggtccccg
     9121 tggcggccgc actcgccggg cgcgccggcc aggggcgcct cgcctgcgtg gtctccggag
     9181 ggaacatcga cccccagaag ctcgcgacca tcctcatggg cgggctcccc tagccttccc
     9241 cgcaggactc ccgattcaag gttttcgccc ctcgtaccga tgttacggat ggaacccgac
     9301 gttcgaaacg gcgtttcacc gagcaggccc cattcccgtg cacgatctca atccacaaga
     9361 gcttcgacgt gccatcctca cgcaccacct ggctcggctc acctgggtgg cgttcatcat
     9421 cacgttcgtc atcgcccggt ccatcgcgct cctccggacc gacttccaca tcatggtggg
     9481 cgaaacgcat ctccaccatc tcaacgtcgg catcattctc ctggccgcga tggggggcta
     9541 cctcctcttc cgccgccccg gcggccacga gcttcggtcg gcggccgtcc tctacggcgt
     9601 ggcgctcggg ctcaccttcg acgagttcgg catgtggctg cacctcgagg acgaatactg
     9661 gcagcgggcg agctatgacg cggtgggggt gatcggggcg ttcctcgggc tgctcgcggc
     9721 gggacccaca ctccggcgcg tgcggcggaa gtactgggcc acggtggccg gtgcggcgat
     9781 cgtggcggtg ctcttcgggc tcctcgtcct caagcccttc ttcagcccgg gctgagcgcg
     9841 ccgggccggc gcgctagcgg agcagcaccg tccgggcgct ggaaaccacc ccggcggcct
     9901 ggagcctgac ccagtagacg ccggacggag cgggcgctcc gtcatcgagc cgtccatccc
     9961 atccgagagg ggtccggccg gccggcatgg tcccgcggtg gagcgtccgc accttgcgtc
    10021 ccctcacgtc gaacacgtcg acgctcgcat cggctggagc cggcaggtcc acctcgagca
    10081 cgagctggcc gtagaacggg aggcgtccca tgagccggag ctgcacgctt gcgggaggtc
    10141 ctccagtatc aacaccgacg accgaggcct tggtgacgat ccggaagaca tcgccgtctt
    10201 gatcgcagat gtaaagctcg ccacgcgcat cctcgccgaa gctggtcacg aacccgatcg
    10261 aatattggcc gacgccccct acaggagcga gttccgcggt gcgatccacg atgttggtga
    10321 gcgagccctg ctggaatgtc cccgaccaga tctgcttcga gcagtagtcg gcatagaagt
    10381 agctgccttg aagcgcgggg atcttggagc cgcggtacac gtagccaccg gtgatcgagc
    10441 acttgccgcc ggtgtgcccg tattccgtcg ctggagcgat gaacggacag gacggattcg
    10501 agcaggagcc gcacggcgtg gtcgtgctcg tggcgtaggg gagggaccct tcgcgacacc
    10561 tccacccgag gttcgcgccc gctcccgtgc cggccgggaa aaagtcgacc tcctcgctcg
    10621 acgactgccc cacgtcggcg atgatgaagt cgtgcgtctg ccggtcgaag ctgtcgcgcc
    10681 acgggttccg gaggccgaag caccagatct cgtcgagccc gggcgtcgcg cccgcgtacg
    10741 gattgccggg cggaatcgta tagcccgatc cgctcacgtc gatccggagg atcttaccga
    10801 agaacgtgtt caggtcctgc gcgcggtccc ctgggtcccc ctcgtcgccg ccgtctccca
    10861 tggcgatgta gaggtatcca tcgggtccga atccgatcca gccgccgtag tggttggcgt
    10921 agggatgcgg gatcgacagg atcaccgtgc cattggggtt ggccacgttg gcatcggcgc
    10981 tcacctggta gcgcacgatc tgcgtgctgc ccgccgcgtc tgtgtagtag acgtagaagc
    11041 gccccgtttg cgcgtagttt ggggcgaacg ctagcccgag gaggccctgc tcggtgctcg
    11101 aggagaggac gtcagtcgtg aggaaaggcg tcgggagcac ggcgccgttc ctcacgatct
    11161 tgatccgccc ccgctggtcg gagccgcgcc gctccacgac gaagatccgc gtcgtgtctc
    11221 cgggcggcgc ggtcacgtag agcggggtgt tgagaccgct cgcgacgcgc acggtggtga
    11281 gcgtcggaac ttgtgcgcgg gcgggtccgg ccgcgagaac ccacgccgcc atccccgcaa
    11341 ggatcagcgc gagggcggag cctctccgtg cgcccatcaa tgcggtctcc gggcgctgtt
    11401 cgcggattcg cgccaccgat cccgccaccc cgagatcatg acgctctcgt cccccggcgc
    11461 gtcccccacg acctggagca cttcgcccga gggatcgatc acgaccgtgc ggtgcgcggt
    11521 cgtgatcatg tcgaaggccc catcgtcgat gccccagaag agattcatgt tggccacgta
    11581 gacatcgttg aattgggacc cctccagacc gaatcgatcc ttgtgatcga tcgtcgccca
    11641 gtaggtgagc tcgctcttca tgggggctcg tgttccggtg ttcagataga ggttgcgccg
    11701 gtagtgcgcg cagagatcgg cgtagtagcg cacggcccgc cacgtggcgg cttgcggtat
    11761 ccggttgggc ccgacgccac gggaccacca caccaccgcg tgcgccgtgt cggcccccat
    11821 ttcccagcga tggtcgagcg cattcacgac cagaggatcc ctctcggggt acgtggactc
    11881 ggcgatcgcg agaaggcgcg cgctcagggt gtcgctggcg gcgcgatcca cggacacgct
    11941 gaacgcgtat gccgtctcgt cgagcctgaa cggacgatcg ccgtagcctt ccgcctcggc
    12001 ctgatcgacg aaatagccgg ggggcgtggc cctggcttgc atcggccgcc cgagggcggc
    12061 cagagctgcg atcggcagta ggacggcgag cgcggagacc cgccaccggc attggattcg
    12121 gtccatgcgt cacctcggag cccggacggt tcccgtcaat gatgcgccgg gcgccagcgc
    12181 cacgccaagg gcaaatgtcc agccccgggg ccccgaaaca gggggttgcg ccccgggggg
    12241 ctcccggtag aatcgatgtc tcgattcgga ccagggtccg atcctcggcg ccggcattca
    12301 ccccgttgtt gggaggtaca ccctaatggc atcgaccaaa cccattcccg atggcttcac
    12361 cacggtcacg ccgtcccttt gtttgaagga ctgcaacaag gcgatcgact tctacaagaa
    12421 ggccctcggg gcgcaggagc tgatgcgcat gccggggccg gacggcaaga tcatgcacgc
    12481 ggagatcaag gtcggtaact cccacgtcat gatgaacgac gagatcatgg gctcgagatc
    12541 ggccgagacc ctcggcgcct cgcccgtgaa cttctatctc tacgtcgaag atgccgacgg
    12601 cgcgcacaag aaggcgatcg ccgccggctg caaggagatc atgccggtcg cggacatgtt
    12661 ctggggcgat cgcatgggcg cggtctccga cccgtacggc tacaagtgga acttctccac
    12721 gcacatcaag gacctgagtc ccgaggagat caagaagggg caggacgagt ggatgaagca
    12781 gatggcgggc tcccgctagg aaaccgcctc atcgatgcga cggagccctt cggccacgcg
    12841 gccggagggc tctcgtcgtt tcagtggagc agcaccacct tgcgtgtcag ggacgcgcct
    12901 tccgattcga tgcgatagaa gtacgctccc gccgccacga gcacgccccg tccgtccgtg
    12961 ccgtcccagc gaagcgagct cgatccctgg ggcaggagtc cgcgccgaag ctcccgcacg
    13021 agccgcccgt ggacgtcgta cacggcgatt cgcccttcgc gcggacgggc gagatcgaaa
    13081 tcgatcgcgg ccgaggaacg gaagggattc gggtaggccg catggagcgt catcgccgca
    13141 ggcgcgctcc cggccggagc ctcggccacc gccgtcacct ggccgaagag gagctcgtac
    13201 gtgtcattct ggacggctcc gtccagcccg ccgtagacga gcatgctctg gcgcacggga
    13261 tcgtagatcg ccacatggcc gctgcgagac gacggcgaga gtcccgttgg ctggagcgcc
    13321 gtccaggtcg gcgtacccgt gagggagagg ccccaggtgt caccgcagaa ggtcgtgccg
    13381 tcgaatccgc cgaagacgac cagccggtcg cgcgactgat cgtagatcgc ggtcggctgg
    13441 tagcgcggcg agggagccgt gcccgatgcg ctgatcgagg tccaggcggg cgcccccgag
    13501 agcgacagcg accacacgtc attgaacagc gcgctgtccg tgcccccgaa cagcaccatc
    13561 gaattcgtga ccggattccg cgtcgaggct gcgaggaacc gggcggccgg cggcgtgcct
    13621 gagggtgtga gctggctcca cgtgggcgtg cccgaaaggg agagggccca cacgtcgctg
    13681 tagaggaggt cgcccaaacc cccgaagatc acgaggcggt ctcccgccgg atcgtagacg
    13741 ccgcacgcgc cgaagcgctc gggaggcgga gtaccgaccg gagtgagctt ggtccacgcg
    13801 ggtgacgcgc cgagcgtgag gctccagacg tcgttcagct gatggttccc gacgtcggac
    13861 ccgccgaaca cgagcatgcg gtcgtgggtc gagtcgtaca ccatgaggtg cccgtcccgc
    13921 gcgtccggct tggtcccaga gggattgaga ttgacccacg tctgggttcc gagcacgagc
    13981 gcccacgcat cctgaagcag tccgttcgcg gcctggcccc cgaacacgat catgcggtcg
    14041 cgcgccgcgt cgtaggccgc ggcgtggtcg gtccgcggag gcgccggccc gccaagcggc
    14101 gccagcgtcg cccacgcggt gccgccggac agcgagagcg agagaagctc gttcgtcggc
    14161 tgatccgcga gaccgccgaa gacgagcatc cggtcgccgg ccgtgtcgag caccgccacg
    14221 tgatcgtagc gtcccgcggg gatgttgccg gacggcgtga gctgcgtcca cgcggaggac
    14281 acaaagggaa gcgcccaggt cgtcccgggc ggctctccgc ttccgtcatc gcccccgaag
    14341 acaaccatgc ggtcgcgcgt gacgtcgtag atcgccttgg accccgcgcg cggcggcgga
    14401 ggcgttccgg ttggcgtgac gagcgtccac gtggccgtcc ccgcgaacga gagcgaccag
    14461 agatcgccca agagaccggt gttgttgaca ccgccgaaca cgacgaggcg atcgcgtacc
    14521 gagtcgtaga tcgcggagtg gcgcgctcgg agatcgggcg ggccgcccga cggagccacg
    14581 ttggaccacg ccggcgttcc ggcaagggcg agcgcccacg cgtcgttccg gtaggtgagc
    14641 cccgtctggc cgccgaacac caccatgcga tcgcgcacgg gatcgtagat cgcgctgtgc
    14701 cccgatcgcg acggaggcgg gctccccgtg ggcgagagct gggtccaggc ggggctcgcc
    14761 ccgagcgaga gcgcccacac gtcgccgagc gcccccggcg ccgcgccgcc gtacacgagc
    14821 atgcggtcgc gcaccggatc gtagatcgcg ctcgagccgc ggcgcgcgct cggcggcgtt
    14881 cctgcgaccg cgaggagctt ccaggtcggc gttccggcga ggctcagctc ccagacgtcg
    14941 ttcttgagcg acgatccggc gagcccgccg aagacgagca ttcggtttcg cacgggatcg
    15001 tagatcgcgg catgctgcac ccgcgccgcg ggcggcggga acgagaggaa cgaccacact
    15061 ccgtcgtcgg tcgcgaccgc cgcggacggg gtgagcgatg atgcgaggag cgccgcggcg
    15121 atgaggccgc cgcggatcga tgcgcttcga atcacttcat gccctccagc cgcttgaggc
    15181 cctcctcggc cagccgatcc ttcgggtcga gctggagcgc cttgtggaat gcctcggccg
    15241 cgttgtcctt ctgccccgcg gccaggtaga ccccgcccag accgtcaagg gcgcgcgagg
    15301 aattcgggtt ctgttccacg ttcagtttca tcacccggac cgcgtcctcg aacttccctt
    15361 tgtccgagag actcgtgcct agctcgatca ggctcccgtc cccgaagtca taggcgttcc
    15421 gaccatagaa gcgcgttcgc agggcctggt aggaggcgag cgtgctgtcg atccccgagg
    15481 tctcgtaggt ccatccgagc tcctcgacga gcgtggcggg agtgggacgg ccgtggtggc
    15541 acgtgatgca tcccaccgtg acgcgatgcg tgccctggag cttcatcttc ttcagatcgg
    15601 cacggacatc gccggtcatc ctcaacatgt cgcgggcgat gtccttcttg gggttcttgt
    15661 cggacgcgaa atcgaacgaa gagagcggct tcccttcttc gccctcgtgg cagtagttgc
    15721 agcggacgcc gagcgatcgc gtgaaggcca tcatggcccc gcggagctcc tccttcgacg
    15781 tggtcttggg caggaccttg aggttcttcg gcttgtccgg ccaatcccag gcgggaggtt
    15841 gctgcgcgag agcgtgaccg gcgagcgaaa ggacgagcgc cgcggagaca acgggaacga
    15901 cgacggacgg cggaacggga attcgcatca ggtctcctgt ccggctacgg ttgggagggt
    15961 cagcggaagc catgggagag tccggcgcat gggctcatct cacgaggtgg gtgccggatg
    16021 gacttgcgtt tccacgctaa gcctacgccc cgtgcgggcg ggcgtcaaac gagtgtggcg
    16081 ggtcggggtg cgtggtagtc tcccctccgt gaatccttac atcctcggcc actcggacca
    16141 cgaaacccgg cgcctcatcc tgcagcacca gatctacgga ccgatcaccc gccgcttctt
    16201 cgaggcggct ggaatcgggc gcggcatgaa ggtgctggac atcgggagcg gggcgggcga
    16261 cgtcgcgctg ctcgtcgcgg atctggtggg cccctcgggg cgcgtcgtcg gcgtggacat
    16321 gaacgccgag atcctgaaca ccgcgcggga gcgcgccatg gtcgccggct ggagcaacgt
    16381 ggagttcctg gccggcgatt tcgcggccgt cgtgacggac aaggacttcg acgcggtcgt
    16441 gggacgctgg gtgctcatgc acgtgcccga cccggtgact ctcgtccgga atgccgcggg
    16501 gcggcttcgc gtcggaggga tcgtcgcgtt ccacgagaac gacttcacct acccgcccac
    16561 gaccttcccg cccacaccgg tcgacatcca ggtcggacgc tggctcgtgc ctccgccggg
    16621 cggccggggt cccgagatgc agatgggcac caagctctac accacctatc tggacgcggg
    16681 ccttcccgcg ccccagttcc gcctcgaggc cccgctcggc ggcgggcccg aatggcccgg
    16741 cttcgaatac gtggccgaga ccgcgcgcag cctcctcccc atgctggaga agatcaccgg
    16801 cgtcaagccg gaggagatcc aggtggacac gctgggcgcg cggctcaagg acgacgcgat
    16861 gaagcaccgc gcggttcaga tgctccctct catgttcggg gcgtggacgc gcaaggtctg
    16921 acggtctaga ttcggtccct cgacgtttcg ctttcatccc ggaggatcca tgcgtctcgg
    16981 caccccgctg gcacccggcg ccacgaaggt catgctcctg ggggccggcg agctcggcaa
    17041 ggaagtcctg atcgcgctcc agaggctcgg cgtcgagacg atcgcggtcg atcgctaccc
    17101 gaacgcgccc gggcaccagg tggcgcacct cgcgcacgtg atcgacatga ccgaccccgc
    17161 cgcgatccgc gcggtggtgg aggccgagcg cccgcatctt gtcgtccccg agatcgaggc
    17221 gatcgcgacc gaggagctcg tccggatcga ggaggacggc ctcgcgaccg tgatcccgac
    17281 ggcgcgggcc acgcgtctca cgatgaaccg cgaaggcatc cgccgcctcg tcgcggagac
    17341 cctccgcctc cccacctcgc gctacgagtt cgccgagagc gaggccgagc tcgccgcggc
    17401 cgcctcgagg atcggcttcc cgtgcgtggt caagccggtc atgtcctcgt caggcaaggg
    17461 acagtccgtc gtgtcgagcc cgggcgacgt ggcgcgcgcg tgggagtacg ccgagagcgc
    17521 ggggcgcgtg aagcacggcc gcgtgatcgt ggaggagatg attcagttcg agtacgagat
    17581 cacgctcctc accgtgcgat ccgccggcga gaacggcgcg agccggacct cgttctgcga
    17641 gccgatcggc cacgtgcaag tgcggggcga ctacgtcgag agctggcagc cgcagccgat
    17701 gggcgacgtc gctctcgccc gcgcccggga tatcgcgaag cgggtcaccg acgacctggg
    17761 cggacgcggc gtgttcggcg tcgagctctt cgtgagggga gatcaggtct ggttcagcga
    17821 ggtgagcccg cgcccgcacg acacgggcat ggtcacgatg gcgacgcagg tgcagagcga
    17881 gttcgagctc cacgcccgcg ccgtgctcgg gctcccggtg agcaccgcgc tccgatcccc
    17941 tggagcgagc gcggtgatct acggcgggct cgaggccaga gggatcgcgt tcgaaggcgt
    18001 ggacgaagcg ctccgcgttc cacagacgga cctccgtctt ttcggcaagc cggagtcctt
    18061 cacgcggcgc cgcatgggcg tcgcgctcgc gtggggcgac gacgtgaacg aggcgagggt
    18121 gcgcgccaag ctttgcgcgg gtaaggtgaa gccggtcgag gccgggagcc ccgcgagcga
    18181 ggctcccgtt cccgcctaaa ccatcgttac cgtgatgcgg cctgcgaggc cgcctgatcg
    18241 cggaactcct cgctcgcgta gatcgccact tccacccgcc ggttcttgct ccgcccttcg
    18301 tccgtcgcgt tggacacgat cggctcgtcc tcgccgcgcc ccaccgtccg gatgctgcgc
    18361 cgcacgccgc gtgcgcggag ataatttgca gccgcgtcgg ctctccgctc ggagagtccc
    18421 tggttgtacg attccgtccc cacgtcgtcc gtgtgtccca cgatgaggag atcggagttg
    18481 tcgtactttt cgaggctctc cgcgagcgtg ttcaaattgt gactcgcctc gccgcggacc
    18541 gcgtccgagt cgaagtcgaa gagcaggccc gaggggaagg tcaccaggat cccctcgccg
    18601 acccggtcca ccttcgcgcc ggggagcttc gcctcgagct cctgggcctt ttcgtccatc
    18661 cggtgtccga tcaccgcgcc ggccgcgccg cccaccaccg cgccgatgat cgcgcccttg
    18721 gtcgtcgagc cggcgtactt gccgacgacg ccgcccacgg ccgcgccgcc cgcggcgccg
    18781 acgatcgcgc ccttctgcgt cttgttgagg ctcgcacacc cggtcgtgcc ggcggcaagg
    18841 aacgccgcgg ccgccaggag ggtgccgatc cgcgtgatgc cgctatgcat cgtgttcacc
    18901 tcgtcggggg tctcggtacg aagcgggtcc atccaaccgg ggagccgagg gagcaagacg
    18961 ggtgccaaaa tcgtacaaag gccg
//
LOCUS       DASTKC010000097         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1490036, whole genome shotgun sequence.
ACCESSION   DASTKC010000097 DASTKC010000000
VERSION     DASTKC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490036"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFR25_04095"
     CDS             complement(<1..768)
                     /locus_tag="VFR25_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9940259.1"
                     /translation="MSPDTAARLDALRVTPNGLAPHYSRFRVEERLLLTGHSHQAWPD
                     VSFDGQLEAWADAAEGVDDKWERAEAKAERVRRGFARLLGARPEEIALGQNTHELVVR
                     FLSALPLAARPRLVTTDGEFHTIRRQLDRLAEDGRLEVVKVAAYPAATLVDRLVQAID
                     DRTSAALVSSVLFETAHLVPGLDGALAACRARGAELLVDAYHHLNVVPFSLAGIGDAF
                     VVGGGYKYCQLGEGNCFLRVPPGRDGLRPLLTGWFSEF"
     gene            complement(791..1567)
                     /locus_tag="VFR25_04100"
     CDS             complement(791..1567)
                     /locus_tag="VFR25_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET9940260.1"
                     /translation="MGVTYSTYLRLDELLDQQKPISDEHDEMLFIVIHQVYELWFKEM
                     LHELDYLKGLLERRDDMRAQHTLKRVLTILKVVVAQIDVLETMTPLEFLTFRERLESG
                     SGFQSFQFRELEFLLGMRNSAALARYPEGSPARQRLERRLGEPSLWDAFLQYLVAKGH
                     AVPPSVLSRDRSGAVESAPELHPLLIHVYKTDPTVRALCERLVDLDEGFQEWRYRHVK
                     MVERTIGTRKGTGGSMGAAYLKTTLDHPFYPDLWAIRTEL"
     gene            complement(1584..3842)
                     /locus_tag="VFR25_04105"
     CDS             complement(1584..3842)
                     /locus_tag="VFR25_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HET9940261.1"
                     /translation="MKAAVSGGGPAGLFFALLLKKADPKHEVQVYERNRLEDTFGFGV
                     VFSDATEEALAQADPEVTAAMGSRAHRWDDIEIHYKGTVLRSTGHGFSGLSRKTLLAL
                     LAERCRASGVRLCFEREVKDPEELLREADLVLAADGAQSVVRERYRDHFRPWVDVRPN
                     RFVWLGTTKPFRAFTFHFKNDAHGLWRVHAYQYEPGASTFIVEAREATWRAAGLDRAD
                     EAATIRFCEQLFAQELEGHRLVSNRSIWRAFPTIRNERWSHGNVVLVGDAAHTAHFSV
                     GSGTKLAMEDASALVRALRANASVPAALEAYEAERRPAVESLQRAAQASLQWFEDTER
                     YMDLDPLTFAFTLLTRSLRVTHENLRVRDPKFIETVDARFATEAARRAGTKAPAGPKS
                     PPPMFTPFKLRGLLLPNRVVVSPMCQYTAVDGTPNDWHLVHLGARAVGGAGLVFAEMT
                     DVSPEARISPGCTGLYKEEHAPAWKRIVDFVHAQTPAKMAIQLGHAGRKGSTRLLWEG
                     DNQPLAQGNWPVLAPSAIPYYPHSQTPREMTRADMDQVLGEYERAAHLAIEAGFDLLE
                     IHMAHGYLLATFISPLTNRRDDEYGGPLANRMRFPLEVFDRVRRAWPEERPMSVRISA
                     VDWHPKGHGPEDAVEVARLLKEHGCDIVDVSAGQTIPDQKPVYGRLFQTPFADRIRHE
                     VGIATMAVGNISSYSDVDTILAAERADLCLLARAHLWDPYWTRHAAHELGYPLPWPDQ
                     YESLANYKPRFT"
     gene            complement(3839..>4129)
                     /locus_tag="VFR25_04110"
     CDS             complement(3839..>4129)
                     /locus_tag="VFR25_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9940262.1"
                     /translation="MATDLTAARLLVYRAAYEADRGAERVTLEAAMAKAFATEAAQRV
                     IDDAVQILGGAGVLASSPVDRLYRAVRALRIYEGTTEIQHLVIAGQLVKEAG"
BASE COUNT          629 a         1400 c         1477 g          623 t
ORIGIN      
        1 gaactcgctg aaccaaccgg tcaggagcgg gcgcaggccg tcccgtccgg gaggcacgcg
       61 gaggaagcag ttcccctcgc ccagctggca gtacttgtag ccgcccccca cgacgaacgc
      121 gtccccgatg cccgcgagcg agaagggcac gacgttcagg tggtggtagg cgtccacgag
      181 gagctcggcg ccgcgcgcgc ggcatgccgc gagcgccccg tcgaggccgg gcacgaggtg
      241 cgccgtctcg aagagcacgc tcgacacgag cgcggcggag gttcggtcgt cgatcgcttg
      301 gaccagtcga tcgaccagcg tcgccgccgg ataggccgcc accttcacca cctcgagccg
      361 gccgtcctcg gccaggcggt cgagctggcg ccggatcgta tggaactcgc cgtcggtggt
      421 gacgagccgc ggccgcgcgg cgagtgggag ggcggagagg aaccggacca cgagctcgtg
      481 cgtgttctgg ccgagcgcga tctcctccgg tcgcgcgccg aggagccgcg cgaacccgcg
      541 ccggacgcgc tccgccttcg cttccgcgcg ctcccacttg tcgtcgactc cctccgcggc
      601 gtcggcccac gcctcgagct gtccgtcgaa ggagacgtcg ggccatgcct ggtgcgagtg
      661 gccggttaga aggagccgtt cctccacgcg aaagcgcgag taatggggtg cgagcccgtt
      721 gggcgttacg cgaagcgcgt cgagtcgggc cgcggtgtcg gggctcatcg agcgctcgga
      781 gccgtggagc tcagagctcc gtccggatgg cccagaggtc cggatagaag gggtggtcga
      841 gcgtggtctt gagataggcc gcgcccatcg agccgccggt tcccttgcgg gtgccgatcg
      901 tgcgctcgac catcttcacg tgccggtagc gccactcctg gaatccctca tcgagatcga
      961 ccagacgctc gcagagcgcg cgcacggtgg gatcggtctt gtagacgtgg atgaggagcg
     1021 gatggagctc gggcgcggat tcgaccgcgc cgctccgatc ccgcgagagg accgacggcg
     1081 gcaccgcatg ccctttcgcg acgaggtact ggaggaatgc gtcccacagc gaaggctcgc
     1141 cgagccgacg ctcgaggcgc tgccgcgcgg ggctgccctc cgggtagcgc gcgagggccg
     1201 ccgagttccg catgccgagg aggaactcga gctcccggaa ctggaacgac tggaatccgc
     1261 tccccgattc gagccgctcg cggaacgtga ggaactcgag cggggtcatc gtctcgagca
     1321 cgtcgatctg cgccacgacc accttgagga tcgtgaggac gcgcttcagc gtatgctgcg
     1381 cgcgcatgtc gtcgcgccgc tcgaggagcc ccttcaggta gtcgagctcg tggagcatct
     1441 ccttgaacca gagctcgtac acctggtgga tgacgatgaa gagcatctcg tcgtgctcgt
     1501 cggagatcgg cttctgctgg tcgaggagct cgtcgagcct gagataggtg gaataggtca
     1561 cgcccaagac gccggacctc cgatcacgtg aaccgcggct tgtagttggc gagcgactcg
     1621 tactggtcgg gccatggcag aggatagccc agctcgtgcg cggcgtggcg ggtccagtac
     1681 gggtcccaca ggtgcgcgcg cgcgaggagg cagagatccg cccgctccgc cgcgaggatc
     1741 gtgtcgacgt cggaatagga cgagatgttc cccaccgcca ttgtggcgat cccgacctca
     1801 tggcggatcc gatccgcgaa gggggtctgg aagagccgcc cgtagaccgg cttctgatcc
     1861 ggaatcgtct gccccgcgga cacgtccacg atgtcgcagc cgtgctcctt gaggagccgg
     1921 gccacctcca ccgcgtcctc ggggccgtgc cctttcggat gccagtccac cgcggagatg
     1981 cggaccgaca tcggcctctc ctccggccac gcgcgccgca cgcggtcgaa cacctcgagc
     2041 ggaaagcgca tgcggttggc gagcgggccg ccgtactcgt cgtcgcggcg gttcgtgagg
     2101 ggcgagatga acgtcgcgag gagatagccg tgcgccatgt ggatctcgag gagatcgaag
     2161 cccgcctcga tcgcgagatg ggcggcgcgc tcgtactcgc cgagcacctg gtccatgtcc
     2221 gcgcgggtca tctcgcgcgg ggtctggctg tgcgggtagt acgggatcgc ggacggagcc
     2281 aggaccggcc agttcccctg ggcgagcggc tgattgtccc cttcccacag gagccgcgtg
     2341 gagcccttgc gtcccgcgtg gccgagctgg atcgccatct tggccggcgt ctgggcgtgc
     2401 acgaagtcca cgatccgctt ccacgccggc gcgtgctcct ccttgtagag acccgtgcag
     2461 cccggcgaga tgcgcgcctc gggactcacg tcggtcatct ccgcgaagac gagcccggcg
     2521 ccgccgaccg cccgcgcccc cagatggacg aggtgccagt cgttcggggt gccgtcgacc
     2581 gcggtgtact ggcacatggg cgagaccacg acgcgattcg ggagcaggag gccgcggagc
     2641 ttgaacggcg tgaacatggg aggcggcgac ttgggtcccg ccggagcctt ggttccggcg
     2701 cggcgcgcgg cttccgtcgc gaaccgcgcg tccacggtct cgatgaactt aggatcgcgc
     2761 acccggagat tctcgtgggt cacgcgcagg cttcgggtga ggagcgtgaa cgcgaaggtc
     2821 aggggatcga ggtccatgta gcgctcggtg tcctcgaacc actggaggct ggcctgcgcc
     2881 gcgcgctgga ggctttcgac cgccgggcgg cgctccgcct cgtacgcctc gagagcagcg
     2941 ggcaccgagg cgttggcccg gagcgcgcgg acgagcgcgc tcgcgtcttc catcgcgagc
     3001 ttcgtccccg atcccacgga gaagtgcgcc gtatgggccg cgtcccccac gagaaccacg
     3061 ttcccatggc tccagcgctc gttccggatc gtcgggaacg cgcgccagat ggagcgattg
     3121 gagacgaggc gatgcccctc cagctcctgg gcgaagagct gctcgcagaa ccggatggtc
     3181 gcggcctcgt ccgcgcggtc gagcccggcc gcgcgccagg tggcctcgcg tgcttccacg
     3241 atgaacgtcg aagcccccgg ctcgtactgg taggcgtgca cgcgccagag cccgtgcgcg
     3301 tcgttcttga aatggaacgt gaacgcgcga aaaggcttcg tcgtgccgag ccagacgaag
     3361 cggttgggac gcacgtcgac ccagggacgg aagtggtcgc gatagcgctc gcgcacgacc
     3421 gactgcgccc cgtcggcggc gagaacgagg tccgcctcgc gcaggagctc ctcgggatcc
     3481 ttcacctcgc gctcgaagca gaggcgcacg cccgaggcac ggcagcgctc cgcgaggagc
     3541 gcgagcagcg tcttccgcga gaggccggag aatccgtggc ctgtcgagcg gagcaccgtc
     3601 cccttgtagt ggatctcgat gtcgtcccac cggtgggccc gcgatcccat cgcggcggtc
     3661 acctcgggat cggcctgcgc gagcgcctcc tcggtcgcgt cggagaagac gacgccgaag
     3721 ccgaacgtgt cctcgagccg gtttcgctcg tagacctgaa cctcgtgctt gggatcggcc
     3781 ttcttgagga ggagcgcgaa gaagaggccc gccggcccgc cgccgctcac ggccgccttc
     3841 acccggcctc cttgacgagc tgccccgcga tcacgaggtg ctggatctcg gtggtcccct
     3901 cgtagatccg gagcgcccgc accgcgcgat agaggcgatc caccgggctc gaggcgagca
     3961 cccccgcgcc tcccaggatc tgcacggcgt catcgatgac gcgctgcgcc gcttcggtcg
     4021 cgaacgcctt ggccatcgcc gcttccaggg tgacgcgctc ggcgccgcgg tcggcctcgt
     4081 acgcggcgcg atagacgagg aggcgcgccg ccgtgagatc ggtcgccat
//
LOCUS       DASTKC010000098         4367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_307841, whole genome shotgun sequence.
ACCESSION   DASTKC010000098 DASTKC010000000
VERSION     DASTKC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307841"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFR25_04115"
     CDS             <1..481
                     /locus_tag="VFR25_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET9940263.1"
                     /translation="EELRPLLTGWFSEFAHLTERTPGRVPYGAGPARWSGATYDPTSH
                     YRAASVFRFFEEMELDPEFLRAVSRHQIGLLAESFDALGLDPRRITRDRQVPLEAVGG
                     FLAFRAPEAGRIAATLRARGMLTDHRAEWLRLGPAPYLSDDQLTAAIDLLGEAVRGL"
     gene            complement(500..1486)
                     /gene="egtD"
                     /locus_tag="VFR25_04120"
     CDS             complement(500..1486)
                     /gene="egtD"
                     /locus_tag="VFR25_04120"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET9940264.1"
                     /translation="MSGRVVTAGVSPGLDGQDNARAVRAGLSGAGRKSLPSRLLYDDL
                     GSALFEAITRLPEYGLTRADERLLTQHAAEIATFAHPPLALVELGSGSGRKARPILEA
                     MARRQSSVTYRPIDLSEAALDQSRVELGGIEGVVVEPILASYGDGLSHAVGASRPGER
                     LLVLFLGGTIGNFDRDEAVRFLADIRSRLRPGDALLVGADLMKPAPQLIAAYDDALGV
                     TAAFNKNVLVRLNRELAADFDPAQFDHEARWNEAERRVEMHLVSKIAQSVALGALGSI
                     IHFKARETIWTESSYRYEKEELPAMAARAGFDCLAQWVDATWPFAETLLTPR"
     gene            complement(1483..2799)
                     /locus_tag="VFR25_04125"
     CDS             complement(1483..2799)
                     /locus_tag="VFR25_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9940265.1"
                     /translation="MATTTRDRSEPTTLAQRLADARTRTDDLFSILRAGALLERPIPE
                     RHRLIFYLGHLEAFDWNLIARQGHGLPAFREDFDKLFAFGIDPVDGGLPNEPASAWPA
                     EAEVRAYNREVRERVDACVLGAQEPRSEHDRQTLVSMASEHRLMHAETLAYLLHQLPH
                     ALKDPRQDPPAPRGTVGAKPHLIAIPDGAATLGQEPGAFGWDNEFDQHVVRVPGFGIE
                     EHKVTNGRFLEFVKDGGYVARELWGDEDWGWKERHLVRRPAFWNERDGVFSYRGMFAE
                     LPLPLEAPVYVSQAEASAFARWAKASLPTEAEFHRAAYGTPDGTEHAYPWGADAPDER
                     HGNFDLRRFDPTPVGSHPGGASAFGVQEMVGNGWEWTRTPFAPFTGFRPHPFYAGYSK
                     DFFDGKHVVMKGASPRTAACFVRRSFRNWFQPRYPYVYATVRLVRP"
     gene            complement(2780..>4367)
                     /locus_tag="VFR25_04130"
     CDS             complement(2780..>4367)
                     /locus_tag="VFR25_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9940266.1"
                     /translation="LGVHRRAGAAGSARMVAVKRAVAMIAALLACLPVARARAAEGGI
                     VVGSKAFPESWILGEALTRTMLGEGIPRVTHRHNLGGTEIVAQGLRSGAIDVYPEYTG
                     TIQEVLLHQSGTVGADSMRAALAALGLGMSDPIGFNDGYAIAIPESAAARLGVRTISD
                     LGRHPDLRLGLTHEFLGRADGYPGLARHYGLSMRHVVGIQHELAYTALASGRIDATDI
                     YTTDAQIARLRLRVLQDDRRFFPRYDAVWLYRLDLPAREPRALQAIRSLTGRISEEAM
                     IRANARVVLDGISYAAAAESLLTEVRPSSAGPAALEREGVASSIARNTLRHVELVLLA
                     LFLAIAIGLPLGIAAAGSRRLGGAILLATGILQTIPSLALLAVLIPLLGIGFRPALAA
                     LFLYSLLPIVRNTVVGLSTLPASLRDAAESLPLSPWARLTRIRLPMAAPAILAGIQTS
                     AVLCVGSATLAALVGAGGLGEPILSGIQLRQTHLILEGAVPAAALALLAQKGFDLLER
                     VAVPRGLRRPEEPTHGDDHA"
BASE COUNT          663 a         1485 c         1587 g          632 t
ORIGIN      
        1 ggaggagctg cgcccactcc tcaccgggtg gttcagcgag ttcgcgcacc tgaccgagcg
       61 cacacccggc cgcgtgccct atggcgcggg acccgcgcgc tggagcggcg ccacctacga
      121 tcccacgagc cactaccgcg cggcctccgt gttccggttc ttcgaggaga tggagctgga
      181 tccggaattc ctgcgcgcgg tgagccggca ccagatagga cttctggcgg aatcgttcga
      241 cgcgctcggt ctggatccga ggcgcatcac gagggaccga caggttccgc tcgaggcggt
      301 gggaggcttc ctcgccttcc gggctccgga agcggggcgc atcgcggcca cgcttcgagc
      361 gcgcgggatg ctcaccgatc atcgcgccga gtggctacgt ctcgggccgg cgccgtacct
      421 ctccgacgat cagctcacgg cggcgatcga tctactcggg gaggcggttc gcgggttgta
      481 gtcgcggcga agcgccgcgt cagcgagggg tgaggagcgt ctccgcgaac ggccacgtcg
      541 catccaccca ctgcgcgagg cagtcgaatc ccgcgcgcgc cgccatggcc ggaagctcct
      601 ccttctcgta ccggtaggag ctctcggtcc agatcgtctc ccgcgccttg aagtggatga
      661 tcgatccgag cgcgccgagc gccacgctct gcgcgatctt cgagacgagg tgcatctcga
      721 cccgccgctc ggcctcgttc catcgagcct cgtggtcgaa ttgggctggg tcgaagtccg
      781 ccgcgagctc ccggttcagc cgcacgagga cgttcttgtt gaaggccgcg gtcacgccga
      841 gcgcgtcgtc gtaggcggcg atgagctggg gcgccggctt catgagatcc gctccgacca
      901 ggagtgcgtc gcccggccgc agccgcgagc ggatgtccgc caggaagcgg acggcctcgt
      961 cgcggtcgaa attgccgatg gtgcctccca ggaacaggac caggagccgc tcacccggcc
     1021 tgctggcgcc aaccgcgtgg ctcagtccat cgccatagct ggcgaggatc ggctcgacca
     1081 cgactccctc gattccgccg agctcgaccc ggctctggtc gagcgccgcc tccgagagat
     1141 cgatgggtcg gtaggtcaca ctggattggc gcctcgccat cgcctcgagg atggggcggg
     1201 ccttccgccc ggatcccgag ccgagctcga ccagcgcgag tggtgggtgc gcgaacgtgg
     1261 cgatctcagc ggcgtgctgg gtgaggagcc gctcgtcggc ccgcgtgagt ccgtattcgg
     1321 gcaggcgtgt gatggcctcg aagagcgcgg agccgaggtc gtcgtagagg agccgcgacg
     1381 gcagcgactt ccgaccggcg cccgagagcc ccgcacggac cgcgcgcgcg ttgtcctgcc
     1441 cgtcgaggcc gggcgaaacc cccgccgtga cgacgcgccc gctcacggtc gcaccagcct
     1501 gaccgtcgcg tacacgtacg ggtagcgcgg ctggaaccag ttccggaacg agcgccggac
     1561 gaagcaggcg gccgtcctcg gagatgcccc cttcatgacg acgtgcttgc cgtcgaagaa
     1621 gtccttcgag tagccggcat agaagggatg cggacggaat ccggtgaacg gcgcgaacgg
     1681 cgttcgggtc cactcccatc cgttgcccac catctcctgc acgccgaagg cgctcgcacc
     1741 gcccggatgg gaaccgaccg gagtcggatc gaagcgccgg agatcgaagt tcccatgccg
     1801 ctcgtccggc gcgtccgcgc cccacggata ggcatgctcc gtaccgtcgg gagttccgta
     1861 ggccgcccga tggaattcgg cctcggtcgg aagggacgcc ttggcccacc gcgcgaaggc
     1921 cgaggcctcc gcctggctca cgtagaccgg cgcctcgagc gggagcggca gctccgcgaa
     1981 catgccgcga taggagaaca cgccgtcccg ctcgttccag aacgcggggc gccggaccag
     2041 gtggcgctcc ttccagcccc agtcctcgtc gccccagagc tcacgcgcga cgtacccgcc
     2101 gtccttcacg aattcgagga agcggccgtt cgtcaccttg tgctcctcaa taccaaagcc
     2161 ggggacgcga accacgtgct ggtcgaactc gttgtcccat ccgaacgcgc cgggctcttg
     2221 gcccagggtc gccgctccgt cggggatcgc gatcaggtgc ggcttcgctc cgacggtccc
     2281 tcgcggcgcc ggcgggtcct ggcgcgggtc cttgagcgcg tgggggagct gatggaggag
     2341 gtaggcgagg gtctcggcgt gcatcaggcg gtgctcgctg gccatcgaca cgagcgtctg
     2401 ccggtcgtgc tcggaccggg gctcctgggc gccgaggacg caggcgtcca ccctctccct
     2461 cacctcgcgg ttgtacgcgc gcacctccgc ttccgcgggc catgcggatg ccggctcgtt
     2521 ggggagcccg ccatcgaccg ggtcgatgcc gaatgcgaag agcttgtcga agtcctcgcg
     2581 gaacgccggg aggccgtgcc cctggcgggc gatcaggttc cagtcgaagg cttcgagatg
     2641 gcccagatag aagatcagcc ggtgccgctc ggggatgggg cgctcgagga gcgcgccggc
     2701 acggaggatc gagaacaggt catcggtacg agtccgagcg tcggccagcc gctgggccag
     2761 ggtcgtgggc tcggaacggt cacgcgtggt cgtcgccatg ggtcggttcc tcgggtcgtc
     2821 gaagcccgcg cggtaccgcc acccgttcca ggaggtcgaa gcccttctgg gcgaggagcg
     2881 cgagcgcggc cgcgggaacc gcgccctcga ggatcaaatg agtttgacgc aattggatgc
     2941 cggagagaat cggttctcct agccccccgg ccccgaccag ggccgccagg gtagccgaac
     3001 cgacgcagag gacggcgctc gtctggattc ccgccaggat cgctggggcc gccatgggga
     3061 gccggatccg ggtcaggcgg gcccaggggg agagagggag cgattcggcc gcgtcccgga
     3121 gggaggcggg gagggtcgag agccccacca ccgtgttccg gacgatgggg aggaggctgt
     3181 agaggaagag cgcggccagg gcgggccgga agccgatgcc cagaagcggg atcaggaccg
     3241 ccaggagcgc gagcgatggg atggtctgga ggatgccggt cgccaggagg atcgcgcccc
     3301 ccaggcgtcg cgagcccgcg gccgcgatcc cgagcgggag cccgatcgcg atagcaagga
     3361 agagcgcgag gagcacgagc tcgacgtgtc ggagcgtgtt ccgagcgatg gacgaagcga
     3421 cgccctcgcg ttccagagcc gccggccccg ccgatgatgg ccggacctcg gtgagcaggg
     3481 actcggctgc ggcggcgtag gagatcccgt cgaggaccac gcgcgcgttc gcgcggatca
     3541 tggcctcttc cgagatccgc cccgtcaggg agcggatcgc ctggagcgcg cgcggctcgc
     3601 gcgccgggag atcgagccgg tagagccaga ccgcgtcgta gcgggggaag aaccggcggt
     3661 cgtcctggag cacgcgcagg cggaggcgcg cgatctgggc gtccgtcgtg tagatgtcgg
     3721 tcgcgtcgat ccgcccgctc gcgagcgccg tgtacgcgag ctcgtgctgg atgccgacca
     3781 cgtgccgcat cgagagcccg tagtggcgcg cgagtcccgg atagccgtct gcgcgtccga
     3841 ggaactcgtg cgtgagcccg agcctgagat cgggatggcg ccccaggtcc gagatcgtgc
     3901 gcacgccgag gcgggccgcc gcgctctcgg ggatcgcgat cgcgtagccg tcgttgaatc
     3961 cgatcgggtc gctcatgcct agcccgagcg cggcgagcgc ggcgcgcatc gagtccgcgc
     4021 cgaccgtccc cgattgatgg agcagcacct cctggatcgt gcccgtgtac tcggggtaga
     4081 cgtcgatcgc tcccgagcga aggccctgcg ccacgatctc ggtgccgccg agattgtggc
     4141 ggtgcgtcac ccgggggatc ccctcgccga gcatcgtcct cgtcagcgct tctccgagga
     4201 tccatgactc ggggaacgcc ttcgatccga cgacgattcc accctccgcc gcgcgagctc
     4261 gcgccacggg caggcaggca aggagggccg caatcatggc cacggcgcgc ttcaccgcga
     4321 ccatccgagc gcttccggcg gcgcctgcgc ggcgatgaac tccgaga
//
LOCUS       DASTKC010000099         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1726677, whole genome shotgun sequence.
ACCESSION   DASTKC010000099 DASTKC010000000
VERSION     DASTKC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726677"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFR25_04135"
     CDS             <1..505
                     /locus_tag="VFR25_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET9940267.1"
                     /translation="ADSVKLERQLHDVKTNKEYQAILHEIEGIKSKRSDYETRILESF
                     EREETLTAGVKAAEKKVAGEEAKLKQGEEDLVREGAELDQRLHSMTVDRDAIKPRVPA
                     ALLSRYDRLLGARDGVAVAEIKKGACGACFKGLTPHALQEARRGDQIKVCEACGRILI
                     LTEAPVT"
     gene            625..>2264
                     /locus_tag="VFR25_04140"
     CDS             625..>2264
                     /locus_tag="VFR25_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940268.1"
                     /translation="MKRHKIALVAGAVCVLLTNLFQVWSPWLVRRAVDHLQHGITKNA
                     VLVDAGLILLAVTLQGIFLFIMRMTIIRASRTMEYELRNDLFAHVARLPAETYRRWKV
                     GDLLSRATNDLDAVRNFLGPGIMYFANTLVMFVMAVTLMLRIDVKLTLVSLIPLPILS
                     FVVARLGGSLHKLYESIQAQFSTMTAKAQETLSGIRVVKAHVEEEGEYEAFRKIHVGY
                     LEENRKMIRLWSMMWPMMSLLGGIAGAIVLAVGGALVVRGTITLGEMVAFQIYLAMLI
                     WPMIALGWVSNLFQRGAASMTRIQVVLDLPIEGDVDPPGVEPVPGARQPRAVAVGAPP
                     AFDGSIEFRAVGYRYPETERWVLRGLDLRVKPGESVALVGRTGAGKTTLLHLVERLFE
                     PTEGVVLLGGVPAAEWPRRALRRSLGIVPQESFLFSDTIKANIGFGFDDGEAAPDAEA
                     VARQAGLGSDLSQFPQGLETLIGERGITLSGGQKQRVALARALALGRRALILDDAFAS
                     VDPGTEEEILETLFGLPTKPTILLATHRRSALLRVDRVV"
BASE COUNT          361 a          736 c          764 g          403 t
ORIGIN      
        1 ggccgactcc gtgaagctcg agcggcaact ccacgacgtg aagacgaaca aggaatacca
       61 ggcgatcctc cacgagatcg aagggatcaa gtccaagcgc tccgactacg agacgcggat
      121 cctcgagtcc ttcgagcgcg aagagacctt gaccgccggg gtgaaggcgg cggagaagaa
      181 ggtcgccggt gaggaagcga agctcaagca gggcgaggag gatttggtgc gcgagggggc
      241 cgaactggac cagcggctcc attcgatgac cgtcgaccgc gacgcgatca agccgcgcgt
      301 tcccgcggcg ctcctcagcc ggtatgatcg actcctgggt gcgcgggacg gggtcgcggt
      361 agcggagatc aagaagggcg cttgcggggc gtgcttcaaa gggctcacgc cgcacgccct
      421 ccaggaggcc cgccgcggcg atcaaatcaa ggtctgcgag gcctgcggcc gcatcctaat
      481 actgaccgag gcgccggtga cgtaggccgt cccggcctcg ctccccggcc gccgggcgcc
      541 catggccccg gccggtcgtc atttcagacc cccacccgct acggagtttc ggccattcgc
      601 gttcttcgcc agttattgcc gtatttgaag cgccacaaga tcgcgctcgt ggccggcgcg
      661 gtctgcgttc tgctcacgaa cctcttccag gtctggtcgc cgtggctggt tcgccgcgcg
      721 gtggatcatc tccagcacgg gatcacgaag aacgcggttc tggtggacgc gggactcatc
      781 ctgctcgcgg tgaccctcca ggggatcttc ctcttcatca tgcgcatgac gatcatccgc
      841 gcgtcgcgca cgatggagta cgagctccga aacgacctct tcgcccacgt cgcgcggctg
      901 cccgcggaga cctaccggcg gtggaaggtg ggcgatctcc tgtcgcgcgc caccaacgac
      961 ctcgacgccg tccgcaactt cctcggcccg ggcatcatgt acttcgccaa cacgctcgtc
     1021 atgttcgtga tggcggtgac cctcatgctc cggatcgacg tgaagctgac gctcgtttcc
     1081 ttgatcccgc tcccgatctt gtccttcgtg gtcgcgcggc ttggcggctc gctccacaag
     1141 ctctacgagt cgatccaggc acagttctcg acgatgaccg cgaaggcgca ggagaccctg
     1201 tccgggattc gcgtcgtgaa ggcacacgtg gaggaggaag gggagtacga ggcgttccgg
     1261 aagatccacg tgggctatct cgaggagaac cggaagatga tccggctctg gtccatgatg
     1321 tggccgatga tgtcgctcct gggcgggatc gccggcgcga tcgtcctggc cgtgggcggc
     1381 gccctcgtcg tgcgcgggac gatcaccctg ggcgagatgg tcgccttcca gatctacctc
     1441 gccatgctca tctggccgat gatcgcgctc ggctgggtct cgaacctatt ccagcgcggc
     1501 gcagcgtcga tgacccggat ccaggtcgtg ctcgatctac cgatcgaagg cgacgttgat
     1561 ccgcccggcg tggagccggt gccgggagcg aggcagccgc gtgcggtggc cgtgggcgct
     1621 ccgcccgcgt tcgacggctc gatcgagttt cgcgccgtgg gttatcgcta tccggagacc
     1681 gagcgctggg tcctgcgcgg cttggatctt cgcgtaaagc ccggcgagtc cgtggccctc
     1741 gtaggacgga ccggcgccgg caagaccacg ttgctgcatc tggtggagcg actgttcgag
     1801 ccgacggaag gtgtcgtgct gctcggaggc gttcccgccg cggagtggcc gcgccgcgct
     1861 ttgaggcgct cgctcgggat cgtgccgcag gagagcttcc tcttctccga caccatcaaa
     1921 gcgaacatcg gcttcggctt cgacgacggc gaggccgcac cggacgcaga agctgttgcg
     1981 aggcaagcag ggcttggttc cgatctctcg cagttcccgc agggtctcga aaccttgatc
     2041 ggcgagcgtg gcatcacgct ctcgggcgga cagaagcagc gcgtcgccct ggcgcgcgct
     2101 ctcgctttgg gtcggcgcgc gttgatcctc gatgacgcct tcgcgagcgt cgacccgggc
     2161 acggaggaag agattctcga gacgctgttc ggcctgccga ccaagccgac cattcttttg
     2221 gcgacgcacc gtcgctccgc cttgctgcgc gtggatcgcg tggt
//
LOCUS       DASTKC010000100         9526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1018178, whole genome shotgun sequence.
ACCESSION   DASTKC010000100 DASTKC010000000
VERSION     DASTKC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018178"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..627)
                     /locus_tag="VFR25_04145"
     CDS             complement(106..627)
                     /locus_tag="VFR25_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9940269.1"
                     /translation="MIAPFGGRTPDVPRSVFVAPTAVVVGDVVLGEDTSVWYGCVIRG
                     DVGPIRVGARTNLQDGCILHVTRGRRPLTVGDDVTAGHRAVLHGATIGDRCLIGMGAI
                     VLDDAEIGEETLIAAGSVVLEGTRVPPRSFVAGVPASVRGPIPDPIRATLLTSAANYV
                     ALAREHAPLHASR"
     gene            complement(653..1162)
                     /locus_tag="VFR25_04150"
     CDS             complement(653..1162)
                     /locus_tag="VFR25_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET9940270.1"
                     /translation="MIPVRVGGIAIDERSKSPVVILQEIEGSRVLPIWIGQNEAVAIH
                     MELSGKKYARPLTHDLCVSMIETLKAKISRVVITDLKDTTFFANIYVDGGHGAVAVDA
                     RPSDSIAIALRSRVPIFVVDQVFEKSSQTPKESSTPEKSPEEKAEELRRYLEEMNPED
                     FGKWNPEDL"
     gene            complement(1173..2495)
                     /locus_tag="VFR25_04155"
     CDS             complement(1173..2495)
                     /locus_tag="VFR25_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9940271.1"
                     /translation="MTSSRAASLSLKATLVLAALALGGCSGGPHPEKGPGASSKAGAK
                     VWQPGHPDTLGPVLAIVGTRRITRHEVDSLIATAPSAIQAELRKEEGYKEAVNRLVTQ
                     EIFLQAAQRAHVDQDSIYQAQLKQSSKDLMLRRYYELKLAALPPIPDSSAQAYFNAHV
                     SDYTLLPRARVRHIALPTKAKAAEVRRALVKGALWDDTALKRSTDKQTSDNGGLLGWV
                     TKDSDMINGVGKAPGIRDAAFALPIGDVSQPIKGPKSWHLIRVDEREDATVQPFESAK
                     DRIVSRLKQERRESYGNALTDTLRQFYNVTVFDDSIKVALSPNKTAADMFKEAQAAAT
                     PLQRIDLYRQLVKQFPSDSVSVQARFMIGFTYAEELNEYDAARKEFQEFIKTYGDTEL
                     GASAKWMLENMEKPAPPLEDDDGTGKGSDKKQAPPPPAPKSGSSTRSP"
     gene            complement(2492..2935)
                     /gene="rplI"
                     /locus_tag="VFR25_04160"
     CDS             complement(2492..2935)
                     /gene="rplI"
                     /locus_tag="VFR25_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9940272.1"
                     /translation="MEVILLEDITGLGKRGAAVKVADGYARNFLLPRKKAISAVGNSQ
                     AVFRDRERSRVSREQKQRQAAEALKDRLAQVSLQFSVQVGEEDQLYGSVTVADIHQGL
                     EAQGFAIERRSIRLPEPLKALGMFEVPVHLFQDVEAPVKVWVVRQ"
     gene            complement(2948..3940)
                     /locus_tag="VFR25_04165"
     CDS             complement(2948..3940)
                     /locus_tag="VFR25_04165"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HET9940273.1"
                     /translation="MTSTVEPARPLAVLEGVRVLGALLLGAGMIAIASSDMPAPLPWA
                     YGLLPVGVLWLARRLGLLLGLLAATSTLGAVYAAGVTEPSQLFFVAVLAGSGLCLAAC
                     ARRGVSASTALAVAAAPVLLVAAGYLVFGGLDELSRMLTERMESLRHYEMEHRVSQTI
                     GMSAVEFEQALERTGKVWAMLLPSLFAMKWVIVLAVNCWLASVLFQDADGFPAFAEFS
                     TWRVHPAGAWAAALALALMAIRLSPALEAGYNLAFPLAIAYTIQGFAVARFMGIAFEM
                     KAIVQLAVLFLVAMLPILLVVFLGIGFFDAWYDFRRRAVAGLTGPWGEGDGNQG"
     gene            complement(3944..4186)
                     /gene="rpsR"
                     /locus_tag="VFR25_04170"
     CDS             complement(3944..4186)
                     /gene="rpsR"
                     /locus_tag="VFR25_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9940274.1"
                     /translation="MARDGESRSFRRKFCKLCFDKVDRIDYKDDRRLTRFITDRGKIV
                     PRRISGTCARHQSQITEAVKRARILALLPFTSEQYR"
     gene            complement(4229..4591)
                     /gene="rpsF"
                     /locus_tag="VFR25_04175"
     CDS             complement(4229..4591)
                     /gene="rpsF"
                     /locus_tag="VFR25_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9940275.1"
                     /translation="MKLYETTVIFDPGLEEARVNEELERVTQPIAQAGGEVIEVQRWG
                     KRKLAYMIGKRRDGTYVHVKHKSPPTLVAEMDRRFRLNESVLRHLTVVAPKESPKEEP
                     KTGEEGEKAAAATPAVGE"
     gene            complement(4599..5177)
                     /gene="pth"
                     /locus_tag="VFR25_04180"
     CDS             complement(4599..5177)
                     /gene="pth"
                     /locus_tag="VFR25_04180"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9940276.1"
                     /translation="MVVGLGNPGSEYADTRHNAGFLAVDALAAKLHRPSGWKRTQGSL
                     RMTGRAGRHDVTLVKPQEYMNRSGATVRALLAEGWALPELLVVYDEVYLPLGTIRIRP
                     RGGSGGHQGLESILAVTGSQEFPRLRIGVGPAPRAEDLPDYVLAPFAEAERDEVPRVI
                     EAACDCAFEAVAEGLTKAMNRWNRFGRDDLDE"
     gene            complement(5188..5853)
                     /locus_tag="VFR25_04185"
     CDS             complement(5188..5853)
                     /locus_tag="VFR25_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9940277.1"
                     /translation="MAMHALKGARRSGVGKGVARKLRQAGSIPAVYYGRGEDPIVLTV
                     GLKELEEVIVKAEGSNVIVDLKVDGDGVGDRKALIREIQRDPVGGHILHLDLQHISLT
                     ERITVEVPIVLTGIPIGVKDGGGILEHLLRDVEVECLPTDIPSRLEIDVSELNIGDSL
                     HVSDLKADKVTILTEGERPIAAVVPPTVLEEAKPAEEGEAAAEPELVKEKKETEESGG
                     KEG"
     gene            complement(5878..6822)
                     /locus_tag="VFR25_04190"
     CDS             complement(5878..6822)
                     /locus_tag="VFR25_04190"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9940278.1"
                     /translation="MRSAETQLRLFTGNANRPLAAAICDYLQIDLGDATVDHFADGET
                     FVKINENIRGSDIFIVQPTFAPATNLMELLVMIDAVRRASARRVTAVIPYFGFQRQDR
                     KDQPRVPITAKLVANLITTAGADRVMTMDLHSPQIQGFFDIPFDHLYAAPVLVDHFMR
                     HQVDDVAVVAPDIGSVKMARAYSKRLSGTLAIVDKRRPRPDAVEMMNVIGEVEDKNVI
                     IFDDIVSTASTLVEAARAVREAGAREVYAACTHGVLCGDAVERIVASDVKELVITDSI
                     PHAPGTLPPKIRMLSVAALLGEAIRRIHDEESLSSLFV"
     gene            complement(7119..7193)
                     /locus_tag="VFR25_04195"
     tRNA            complement(7119..7193)
                     /locus_tag="VFR25_04195"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7159..7161),aa:Gln,seq:ttg)
     gene            complement(7200..7493)
                     /gene="spoVG"
                     /locus_tag="VFR25_04200"
     CDS             complement(7200..7493)
                     /gene="spoVG"
                     /locus_tag="VFR25_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HET9940279.1"
                     /translation="MTLEITEVRISLREDDKLKAFASMTFNDAFVIRGLKIIEGTNGM
                     FVAMPSRKRPDGTYQDVAHPINNETRKFIEDRILDEYERERSKQQPSLIGTTL"
     gene            complement(7595..8509)
                     /gene="ispE"
                     /locus_tag="VFR25_04205"
     CDS             complement(7595..8509)
                     /gene="ispE"
                     /locus_tag="VFR25_04205"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET9940280.1"
                     /translation="MAAPLTEGDAAPLRVRCPAKINLGLWILGKRPDGYHEIDTILQT
                     IALEDEITLEPAREGLALTTRGIPIPGDAPNLVTRAWDLLVERAGGRLPRGIRATLTK
                     RIPVGAGLGGGSSDAAGFLLGADRLLGLGLATEELHALAARLGSDVPFFLRGGTARAT
                     GRGDLVRQLCPIDPLWIVLVSPPVAISTTWAYGQVKLGLTRHDDRASFLESALAGRNL
                     EAAWSEMRNDFEDVVLPGFPVLAALRHALIGGGAVKALLAGSGSTLFGIQRTREDALR
                     LAKGLAGRGAQVRAVRTLERGAVVAPLT"
     gene            complement(8494..9396)
                     /locus_tag="VFR25_04210"
     CDS             complement(8494..9396)
                     /locus_tag="VFR25_04210"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HET9940281.1"
                     /translation="MKPLLASMRVYQWTKNLVLFAGLVFTLKILDPTYVIPAIAGFLT
                     FSLAVSGMYLLNDVLDVERDRLHPEKRRRPVASGALPVPAALAAAVVLLAIGLGGSYA
                     IGPRFGSTALAYVLLTLSYSLVLKRVVLLDVVSIALGFVLRATAGVELLRDRAPGGAE
                     SIELSPWLLVCAFFLALFLAIGKRRHELAVLEGDASRHRAALGSYTTKLLDQLVAVVT
                     SATVLAYSVYTIAPETLAKFGGRPLYLTIPFVLYGIFRYLYLMYAEEKGGNPSEHLLS
                     DKATLVNVILWGAAVLAILTWPRP"
BASE COUNT         1529 a         3278 c         3157 g         1562 t
ORIGIN      
        1 gacttcgcgt ccttcggctt cgcggggcct gcggtcgcca cggcgcacac cgcgagcgcg
       61 atcgcgagga gtgccgcggt gggcgcggcc acgaagcggg atcggtcagc gggacgcatg
      121 gaggggcgcg tgctcgcgcg cgagcgccac gtagttggcg gccgaggtga ggagcgtcgc
      181 gcggatcggg tcgggaatgg ggccgcgcac ggatgcgggc acgccggcga cgaacgagcg
      241 aggcggaaca cgcgtccctt cgaggaccac gctccccgcc gcgatgagcg tctcctcgcc
      301 gatctcggcg tcgtcgagca cgatcgcccc catgccgatg aggcagcggt cgccgatggt
      361 cgcgccgtgg agaaccgcgc ggtgccccgc cgtgacgtca tccccgaccg tgagggggcg
      421 gcgtccgcgc gtgacatgga ggatgcagcc gtcctggagg ttggtccggg cgccgacgcg
      481 gatcgggccg acgtcgccgc gaatgacgca gccgtaccag acgctcgtat cctcgcccaa
      541 aacgacgtcg cccaccacga ccgcggtggg cgcgacgaag accgatcggg gcacgtccgg
      601 cgtccttccc ccgaacgggg cgatcacggg cgggccgaca tccggcgcgg ggtcagaggt
      661 cctcgggatt ccatttcccg aaatcctcgg ggttcatctc ctcgagatag cggcgcagct
      721 cctccgcctt ctcctcggga gacttctcgg gcgtcgatga ttccttgggg gtctgcgagg
      781 acttctcgaa gacctggtcg accacgaaga tcggcacgcg cgagcggagc gcgatcgcga
      841 tggaatcgct cggccgagcg tcgacggcca ccgcgccgtg gcccccgtcg acgtagatgt
      901 tcgcgaagaa cgtggtgtcc ttgaggtcgg tgatgacgac gcgggagatc ttggctttca
      961 gggtctcgat catggagacg cagaggtcgt gggtcagagg ccgcgcgtat ttcttgcccg
     1021 agagctccat gtggatcgcc acggcctcgt tctgcccgat ccagatcggc agcacgcgag
     1081 agccttcgat ctcctggagg atgaccacgg ggctcttgga tcgctcgtcg atcgcgatgc
     1141 cgcccacgcg gaccggaatc atgcccatcg gctcagggag acctcgtgga cgaccccgac
     1201 ttcggagcgg gcggcggagg cgcctgcttc ttgtcgctcc ccttgccggt gccgtcgtcg
     1261 tcctcgagcg gcggagccgg cttctccatg ttttccagca tccacttcgc cgacgctccg
     1321 agctccgtgt cgccgtacgt cttgatgaac tcctggaatt ccttgcgggc ggcgtcgtac
     1381 tcgttcaact cctccgcata ggtgaagcct atcatgaagc gcgcctgaac cgataccgag
     1441 tcggacggaa attgcttcac gagctgccgg tagaggtcga tgcgctggag cggcgtggcc
     1501 gcggcctggg cttccttgaa catgtcggcc gcggtcttgt tcggcgagag cgcgaccttg
     1561 atggaatcgt cgaagacggt gacgttgtag aactgcctca gcgtatcggt gagcgcgttg
     1621 ccgtaggact cgcgccgctc ctgtttgagc cgcgacacga tgcgatcctt ggcgctctcg
     1681 aagggctgca ccgtcgcgtc ctcgcgctcg tccacgcgga tcaggtgcca ggacttcggc
     1741 cccttgatgg gctggctcac gtcgccgatc gggagggcga aggccgcgtc ccggatcccg
     1801 ggcgcctttc cgaccccgtt gatcatgtcg gaatccttcg tcacccagcc gaggaggccg
     1861 ccgttgtcgc tggtctgctt gtcggtcgac cgcttgaggg cggtgtcgtc ccagagcgcc
     1921 cccttcacga gcgcccttcg cacctcggcc gccttcgcct tggtcgggag cgcgatgtgg
     1981 cgcacgcgtg cgcgcgggag cagggtgtag tcgctcacgt gcgcgttgaa gtaggcctgc
     2041 gcggaggagt ccgggatcgg ggggagggcc gcgagcttca actcgtagta gcggcgcagc
     2101 atcagatcct tcgacgattg cttgagctgg gcctggtaga tcgaatcctg gtccacgtga
     2161 gcgcgctgcg cggcctggag gaagatctcc tgagtcacga ggcgattcac cgcctccttg
     2221 tacccctcct ccttgcggag ctcggcctgg atggcgctcg gggcggtcgc gatgagcgag
     2281 tccacctcat ggcgcgtgat ccttcgcgtg cccacgatcg cgaggaccgg gccgagcgtg
     2341 tcggggtgcc ccggctgcca gaccttggcg ccggctttgg aggacgcgcc aggacccttc
     2401 tccggatgcg gacccccgga acagcccccg agagccagcg ccgcgagaac gagcgttgct
     2461 ttgagcgaga gcgaagcggc gcgggacgac gtcattggcg cacgacccac actttcacag
     2521 gagcctcgac gtcctggaac aggtggaccg ggacttcgaa catcccgagc gccttgagcg
     2581 gctccgggag gcggatgctc cggcgctcga tcgcgaagcc ctgcgcttcg agcccctggt
     2641 ggatgtcggc gacggtcacc gagccgtaga gctggtcctc ttcgccgacc tggacgctga
     2701 actggagcga gacctgcgcc agccggtcct tgagcgcctc ggcggcctgg cgctgcttct
     2761 gctcgcgcga gacccgcgag cgctcgcgat cgcggaacac ggcctgcgaa ttccccaccg
     2821 ccgagatcgc ctttttccgc ggcagaagga agttgcgcgc gtagccgtcg gcgaccttga
     2881 ccgccgcgcc gcgcttgccc agaccggtga tgtcctccag caggatgacc tccatcgttc
     2941 ctccttatta accttggttc ccgtcgccct cgccccacgg gccggtcagg cccgccacgg
     3001 cgcggcggcg gaagtcgtac cacgcatcga agaatccgat ccccaggaac accacgagca
     3061 ggatcggcag cattgccacc aggaagagca ccgcgagctg cacgatggcc ttcatctcga
     3121 acgcgatccc catgaaccgg gccacggcga acccctggat cgtgtatgcg atcgccaggg
     3181 gaaacgccag gttgtagccg gcctcgagcg ccgggctcag ccggatcgcc atgagcgcga
     3241 gcgcgagcgc ggccgcccac gcccccgccg gatgcacccg ccacgtcgag aactccgcga
     3301 acgcgggaaa tccgtccgca tcctggaaga gcaccgaggc gagccagcag ttcaccgcga
     3361 gcacgatcac ccacttcatc gcgaagaggg acggcaggag catcgcccag accttccccg
     3421 ttcgctcgag cgcctgctcg aactcgaccg ccgacatgcc gatcgtctgc gagacccggt
     3481 gctccatctc gtagtggcgc aggctctcca tccgctccgt cagcatccgg ctcagctcgt
     3541 ccagtccgcc gaacacgaga tagcccgccg ccacgaggag caccggcgcc gccgccacgg
     3601 cgagcgccgt ggaagcgctc acaccgcgcc gcgcgcacgc ggcgaggcag agtcccgacc
     3661 ccgcgaggac cgccacgaag aagagctgcg acggctccgt gacgccggcc gcgtagaccg
     3721 cgccgagcgt ggacgtggcg gccagaaggc cgagcaagag tccgagccgc cgcgccagcc
     3781 agagcacgcc gaccgggagg agcccgtacg cccagggaag cggagccggc atgtcgctcg
     3841 aagcgatcgc gatcatcccc gcgccgagaa ggagcgctcc gaggacgcgg accccctcga
     3901 gcacggccag aggccgcgcc ggctcaaccg tgctggtcac aggtcagcgg tactgttcgc
     3961 tggtgaaggg taggagcgcc aggatgcgcg cgcgcttcac agcctcggtg atctgggact
     4021 ggtgccgggc gcacgtgccg gagatgcggc gcggcacgat cttcccgcga tccgtgatga
     4081 agcgcgtcaa gcggcggtca tccttgtagt cgatccgatc caccttgtcg aagcaaagct
     4141 tgcagaactt ccgtcgaaac gaacgcgatt cgccgtcgcg tgccatgaaa cctcctgttc
     4201 ggtgtcggtc gtaccccgtg gggggacgct attctccgac cgccggcgtc gccgccgcgg
     4261 ccttctcgcc ttcctcgccc gtcttcggct cttccttcgg gctctccttc ggagccacca
     4321 cggtcaggtg ccgcagcacg ctctcgttca accggaagcg gcggtccatc tcggcgacca
     4381 gcgtgggcgg gctcttgtgc ttcacgtgca cgtacgtacc gtcgcggcgc ttgccgatca
     4441 tgtacgcgag cttgcgcttg ccccagcgct gcacctcgat gacctcgccc cccgcctgcg
     4501 cgatgggctg ggtcacgcgc tccaactctt cgttcacccg cgcctcctcg aggcccggat
     4561 cgaaaatgac cgtcgtttcg tacagtttca tcgtctcctc actcgtccaa gtcgtcgcgt
     4621 ccgaatcgat tccagcggtt catggccttg gtgaggccct ccgccaccgc ttcgaacgcg
     4681 caatcacatg ccgcctcgat cacgcgcggc acctcgtccc gttctgcctc cgcgaacgga
     4741 gccagcacgt aatcgggtaa atcctccgcg cgcggagccg gtcccacgcc gatccgaagc
     4801 cgcggaaact cctgcgatcc cgtgaccgcc aggatcgact ccagcccctg atgcccgccc
     4861 gacccgccgc gcgggcggat gcggatcgtt cccaggggca ggtagacctc gtcgtacacc
     4921 acgaggagct ccggaagcgc ccatccttct gcgagcagcg cgcgcaccgt ggcgccgctc
     4981 cggttcatgt actcctgggg cttcacgagc gtgacgtcgt gccggccggc ccgtccggtc
     5041 atccggagcg agccctgggt ccgcttccac cccgaagggc ggtgaagctt cgcggccagc
     5101 gcgtccaccg cgaggaagcc cgcgttgtga cgcgtgtcgg cgtactccga gccgggattg
     5161 cccagcccca cgaccacccg gacctcgtta accttccttg cctcccgact cctccgtctc
     5221 cttcttctcc ttcacgagct ccggctcggc ggccgcctcg ccctcctccg ccggcttggc
     5281 ctcctcgagc accgtcggcg gcaccacagc cgcgatcgga cgctcgcctt cggtcaggat
     5341 cgtgaccttg tcggccttga gatcgctcac gtggagggag tcgccgatgt tgagctcgct
     5401 cacgtcgatc tcgaggcggg acgggatgtc ggtcgggagg cactccacct cgacgtcgcg
     5461 aagcaggtgc tccaggatgc cgccaccgtc cttcacgccg atcggaatac cggtgagaac
     5521 gatcggcacc tcgaccgtga tccgctcggt gagggagatg tgctggagat cgaggtggag
     5581 gatgtgcccg cccaccggat cgcgctggat ctcgcggatg agcgccttgc ggtcgcccac
     5641 gccgtcgccg tcgaccttga ggtccacgat gacgttcgag ccctccgcct tcacgatgac
     5701 ttcctcgagc tccttcaatc cgaccgtgag aacgatcgga tcctccccgc ggccgtaata
     5761 gacggccggg atgctccccg cctggcggag cttgcgcgcg acgcccttgc cgactccgct
     5821 ccggcgcgcg cccttcaatg catgcatggc catggtgctg ctacctccgt tccggtctca
     5881 aacgaacagc gagctcagcg attcttcgtc gtggatccgg cggatcgcct cgccgagcag
     5941 cgccgccact gaaagcatgc ggatcttcgg ggggagcgtg ccgggcgcgt gagggatgct
     6001 gtcggtgatg accagctcct tcacgtccga ggccacgatc cgctccaccg cgtcgccgca
     6061 gagcaccccg tgggtgcagg cggcgtagac ctcccgggcg cccgcctccc ggaccgcgcg
     6121 cgccgcctcg accagggtcg acgcggtgct cacgatgtcg tcgaagatga tgacgttctt
     6181 gtcctcgacc tcgccgatca cgttcatcat ctcgacggcg tccggcctgg gccgccgctt
     6241 gtcgacgatg gccagcgttc cgctgaggcg cttcgagtac gcgcgggcca tcttcacgct
     6301 gccgatgtcg ggcgcgacca ccgccacgtc atcgacctgg tggcgcatga agtggtcgac
     6361 caggaccggc gcggcgtaga gatggtcgaa cggaatgtcg aagaagccct ggatctgcgg
     6421 cgagtgcaga tccatggtca tcacgcggtc cgcacccgcc gtcgtgatga gattcgcgac
     6481 gagcttggcc gtgatcggga cgcgcggttg gtccttccgg tcctgccgct ggaacccgaa
     6541 gtacgggatg accgccgtga cgcgccgcgc ggaggcgcgg cgcaccgcgt cgatcatcac
     6601 gaggagctcc atcaagttcg tggccggcgc gaaggtcggc tgcacgatga agatgtccga
     6661 tccgcggatg ttctcgttga tcttgacgaa cgtctcgccg tcggcgaaat ggtcgacggt
     6721 cgcgtcgccc agatcgatct gcaagtagtc gcagatcgcc gccgccagcg ggcggttcgc
     6781 gttgcccgtg aagaggcgca gctgggtttc cgcggagcgc atccgcggtg cttccggcag
     6841 tgccttggcc gatggcgtcg cgcgtcgatc cgtagccagc ttcaccgtca tcgcctcctc
     6901 ccgggttctt gctcacccat gaaaacgacc ccaaaatgaa aacgacccaa agaccgattg
     6961 acgacttcgt caatcgggcc cgatgcgccg cgcggcgctc gacggaccgg aggactcgcc
     7021 tccggggccc gtgatcgatg tcacgatggt cctgggtcga aaaccgcggc cggccaaccg
     7081 gcccgcgggt aaagcctaga gcatctttcc ccgattcctg gttggggcgg gaggattcga
     7141 acctccgaat gggagatcca aagtctcctg gcttaccact tgccgacgcc ccaacagggc
     7201 tacagcgtcg tcccgatcag ggatggctgc tgcttcgagc gctcccgctc gtactcatcg
     7261 aggattcgat cctcgatgaa cttccgcgtc tcgttgttga tgggatgagc gacatcctga
     7321 tacgtcccat caggccgctt gcggctcggc atggccacga acatcccgtt cgtcccctcg
     7381 atgatcttga ggcctcgaat gacgaacgcg tcgttgaagg tcatgctcgc gaacgctttc
     7441 agcttgtcgt cttccctgag cgaaatccgc acttcagtga tctcgagtgt catgcgtcac
     7501 tccacctcga gttcggaggc cgatccctcg gcgtcccctg acccccacac caggactctg
     7561 gtactcgccc cgccgtgacc ccgccgcctt cccgctacgt gagaggagcc acgacggcac
     7621 cacgctccag agtgcgaacc gcgcgaacct gtgcgccccg tcccgcgagt cccttcgcga
     7681 gccgaagcgc gtcctctcga gtccgctgaa tgccgaagag agtcgatccg ctcccggcaa
     7741 gcagcgcctt caccgcgccg ccgccgatga gggcgtgtcg gagcgcggct agtacaggaa
     7801 accccggaag gacaacgtcc tcgaaatcat ttcgcatctc gctccaggcc gcttccaaat
     7861 tgcggcccgc gagtgcggac tccaggaaac tagcgcgatc gtcatggcgt gtcaatccca
     7921 atttgacctg cccatacgcc caagtcgttg aaattgcgac aggtggagag accaggacga
     7981 tccagagcgg gtcaatggga caaagttgtc tcaccaggtc tccccgcccg gtcgcgcgcg
     8041 cggttcctcc tcttagaaag aagggaacgt cggagccgag acgcgccgcg agcgcgtgga
     8101 gctcttccgt tgcgagaccg aggccgagga gccgatccgc gccgagcaga aatcccgcgg
     8161 cgtcgctgct ccctccgccg agccccgcgc cgacggggat ccgtttcgtg agcgtggccc
     8221 ggatccctcg aggaagccgc ccgcccgccc gctcgacgag gagatcccac gcgcgggtca
     8281 cgagattcgg cgcgtcgccc gggatcggaa tccctcgcgt ggtcagcgcg aggccttcgc
     8341 gcgcgggctc gagcgtgatc tcgtcctcga gcgcgatcgt ttggaggatg gtgtcgatct
     8401 cgtgatagcc gtcggggcgc tttccgagga tccagagacc gaggttgatc ttcgccgggc
     8461 agcggacgcg aagcggcgcg gcgtcgccct cggtcagggg cgcggccacg tgaggatcgc
     8521 gagcacggcc gccccccaga ggatgacgtt cacgagcgtc gccttgtcgc tgaggagatg
     8581 ctcggaggga ttccctccct tctcctcggc gtacatcagg tagaggtagc ggaagatgcc
     8641 gtagaggacg aacgggatcg tgagatagag cggacggcct ccgaacttgg ccagggtctc
     8701 gggggcgatc gtgtagaccg aataggcgag cacggtcgcg ctcgtcacga cggcgacgag
     8761 ctggtccagg agcttcgtcg tgtaggagcc gagcgcggcg cgatgccggg acgcgtcccc
     8821 ttccagcacc gcgagctcgt gccgccgctt tccgatcgcg aggaagagcg cgaggaagaa
     8881 ggcgcacacg aggagccacg gggagagctc gatcgactcg gctccgcccg gcgcgcgatc
     8941 gcggaggagc tccacgcccg cggtggcgcg gagcacgaat ccgagcgcga tcgacaccac
     9001 gtcgagaagg acgacgcgct tcaagacgag cgagtaggag agcgtgagga gcacgtacgc
     9061 gagcgccgtg ctgccgaacc ggggaccgat cgcgtaggag cccccgagcc cgatcgcgag
     9121 gaggacgacg gccgccgcga gggcggccgg aaccgggagc gctcccgacg ccacggggcg
     9181 gcggcgcttc tccggatgga gccggtcccg ctcgacgtcg agcacgtcgt tcagcagata
     9241 catccccgag accgcgagcg agaacgtgag gaatcccgcg atcgcgggaa tcacgtaggt
     9301 cgggtcgagg atcttcagcg tgaagacgag cccggcgaag aggaccaggt tcttggtcca
     9361 ctgatagacc cgcatcgacg cgaggagcgg cttcatcagc gccggcgcgg ctttcgaggg
     9421 cgggatcgga cggcctgctc ggcgcgttcg cagcagaggt gccccaccac gaggtggact
     9481 tcctgcacgc gcggcgtctc ctggctcggc acgacgagcg cgagat
//
LOCUS       DASTKC010000101        14108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1018971, whole genome shotgun sequence.
ACCESSION   DASTKC010000101 DASTKC010000000
VERSION     DASTKC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018971"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFR25_04215"
     CDS             <1..362
                     /locus_tag="VFR25_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET9940282.1"
                     /translation="PVMNPTFAYAVAVGIAAATLYGIARTLGRRDAESDEVAFGPPCE
                     IQVLSRVRLGRGRTLVVVEVEGKRLLLGSTSSEWTALADLGPVGATVAVSDDPFGPID
                     AELARAIQASRSKRSNN"
     gene            580..1578
                     /locus_tag="VFR25_04220"
     CDS             580..1578
                     /locus_tag="VFR25_04220"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET9940283.1"
                     /translation="MSDIENKSKSTPTAKAAPTIRPVGGSKSASRRAPGGKSKRSSKP
                     VVPPPPSRPRRRTIAVTSGKGGVGKTNIVTNLAIALARQGIRVLVLDGDLGLANVDLL
                     LGVAPQFDLQDVILGGRNVRDVVLEGQDGIRVVPASSGVEELANLDEYRTEVLLRSLA
                     ELDGEVDIILIDSPSGIGTHATSLIQGADQILVVTTPEPTSFSDAYAMIKVLVRRPLK
                     CTPALLVNQADSEESAIAVARRVKNVAKRFLNLDVDYWGYVLDDESVPKSVLRQEPFL
                     STYPYSPASSCIYQLARKIMGQQGRNPAPHGGNGGHSGHKDMAETTLSIVDPSEEA"
     gene            1575..1874
                     /locus_tag="VFR25_04225"
     CDS             1575..1874
                     /locus_tag="VFR25_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940284.1"
                     /translation="MIVRRLVIRLTLIASVLAGLAATILGFATGTQPHVVMFRALLAF
                     TIVLLAGAGVGLVVMRTALRRYYEQGRRSGDGRLQTNPVSSNRPVPTDRRVPADR"
     gene            1890..2660
                     /locus_tag="VFR25_04230"
     CDS             1890..2660
                     /locus_tag="VFR25_04230"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HET9940285.1"
                     /translation="MPTPSVLHSLAHRTPRSRNRKQRQKEVALSKYAPLVKYVVDRLA
                     LHLPKSVERDDLISAAIIGLFDALEKYDASKGTKFETYAIWRIRGAILDELRSLDWAS
                     RSIRRKARNVEEVARELGQKLGRAATEEEVAEALNLSPVELSRLMDEVHGTALLSLSK
                     SVSSDEDQDFIQLEDIVDDPSHKDALEVIESDEAREVLLETIDGLPEQQRLVVALYYY
                     EEMTLKEIGEALHISESRVSQIHTRAVKTLKGRLGRLF"
     gene            2663..3634
                     /locus_tag="VFR25_04235"
     CDS             2663..3634
                     /locus_tag="VFR25_04235"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9940286.1"
                     /translation="MTPSRTGTNAPRPSAPRNVGAKPARTNTAAPGTLDRSSLLKLTE
                     SMVALPTLPLVATRLLRAVAQPDTTPDEIARILSLDPALTARTLRLANSEFYGFPRRV
                     GSVDLAVVVLGANPIRDLALSASVFRTIGEADALMEDVWRHSLAAGVAARILADRTRY
                     RLESEAYAAGLLHDIGTVVLRQTDPSRFQAVIAMVRDQGETIEEAERGLFGSCHAEVG
                     AWLAERWGLPADLVEAIACHHRPETARRNPELAALVHVATSLAERAGFAWPRGVARRP
                     IAPSAFEVVDADERKREALLETLVEDVVRETEREQELFAEFRGSQED"
     gene            3640..4008
                     /locus_tag="VFR25_04240"
     CDS             3640..4008
                     /locus_tag="VFR25_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940287.1"
                     /translation="MGSAATKSAHDRDIEIRVRFEPGLGAALYASLRDGEVGAVTVAK
                     NLGRGRSLLRYHGFTLMAQGAPDWKVGDSFSVYVKELGPPLVLAPVGANGRSASRRVA
                     TVESAVVPGVPQSSNNAVGR"
     gene            4025..5260
                     /locus_tag="VFR25_04245"
     CDS             4025..5260
                     /locus_tag="VFR25_04245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET9940288.1"
                     /translation="MKQRNGAGSKRLVEATERFIKSEQKLLEVLGGRRILKKTECDHF
                     AKDLALSAAKKRSSDVFASAISINRKLMGVLSKSRAAAVKTVSESDVLAELGRIAQTV
                     MDPADAFEQALVQMRAVVPYENATLFLWSRETQCLEEAATVGSRVDLIGHVRFGQGAG
                     FSSWVAEQRKPVLLNDLHREGGPDAVALRSFLSVPLLVQNEVVGVINMSHSRPSAFEE
                     EMVTRVALFGLLIASVAMRTVLRKDRERMATTDALTSLYNKRHFDQSLEVEVGRAKRY
                     GHKVSVLVLDVDRPREPKTKIGASAGEQVLSDMGRLLKRSARTTDCIARYGYDEFRIL
                     LPHTDEARAKVAADRIRGVVEKHAFPRRRRPTVHIGIATYPTDSALAPVTTPNLERAV
                     DAVMAPPVDIASPSEAAAS"
     gene            5268..6992
                     /locus_tag="VFR25_04250"
     CDS             5268..6992
                     /locus_tag="VFR25_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940289.1"
                     /translation="MTERTMTPTASSGTDAPRARILVVDDEIQVVHIFRDLLTQQGYE
                     VEDCDNGDDAILKVTNGKFDLVLTDINLPGVDGLEVIRAAKAADKDTCVILITGYAST
                     TTAIDALRQGAYDYITKPFDLWETAKAIERGLESRNLSRENTRLLKQLGDANAELQQH
                     EEILKRKVERATHRMASLYEAGKAISTSLSLQSTLEVIVAQAARLTASNSCLVFLHDT
                     STDEYAAEAGLGVKRESFQSLRFGMGVGYHGQVVQTDKPLLVDELESKVGAEPLFSKL
                     KASSALIVPLRSSESVLGTITCLDHEGGSFTTEDMDVLMMLASQATIAITNAILFERT
                     KELDRLKSEFVAVVSHEVRTPITSIKGSLELLSDERFHKLPAPQKELLSICQANTERL
                     ISLINDILDFSKLESSKLSLNIESIEVDSVLSEAVENIRSLAAQKEVVIDLVVDASVG
                     KIEADPMRVGQVATNLIGNAIKFSPDKSRIEVFASGDPEKITVSVKDYGRGISARDIS
                     RLFQRFAQLDSSTTRKAGGTGLGLVISKGIVEQHGGNIWVESALNEGSTFSFSLPRFR
                     ADGGSPTT"
     gene            7071..8186
                     /gene="wecB"
                     /locus_tag="VFR25_04255"
     CDS             7071..8186
                     /gene="wecB"
                     /locus_tag="VFR25_04255"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET9940290.1"
                     /translation="MTSQPTVLSVFGTRPEAIKMAPVIRRLKQEPGLRSLVCVTAQHR
                     EMLDQVLRWFEIRPDHDLDLMRDGQTLTEITTRALGALTPYLEREKPSLLLVQGDTTT
                     TMAASLAAFYARIPVAHVEAGLRTGDNMYPYPEEVNRRITTVIASRHFAPTEGARANL
                     LREGVDPASILVTGNTVIDALLEVVKQEPKRTPKLPLRGKRLVLVTAHRRENFGAPLL
                     EITGALRDLAERYPDLDLVYPVHRNPNVDGPVRKALGGVAGVHLIDPLEYKPFADLMA
                     ASTLILTDSGGIQEEAPSLGKPVLVLRDETERPEAVEAGVVKLVGPHRDAIVRAASEL
                     LDDPARYRAMAAAVNPYGDGHAAERIVKVIREMLGVG"
     gene            complement(8194..9378)
                     /locus_tag="VFR25_04260"
     CDS             complement(8194..9378)
                     /locus_tag="VFR25_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET9940291.1"
                     /translation="MASIPFHRPSIGQGELDAVAAVLRSGWLTTGPRVKELEAAVVAY
                     VTPPAEAIASGGRGVARGPLHGVAVSSCTAALHLALIAAGVGEGDEVVTTPYTFVSTG
                     ETILYLGAKPVFVDVERDTKNLDPRLLPAAITPRTRAIVSVSMAGHPCRAAEIAAIAH
                     EHKLPMIEDAAHSLTAQIWGVPVGSQADITCFSFYATKGVTAGEGGMAVTAHADWAER
                     MRRLSLHGLSAAAWSRYDEGGWWDYDVTELGYKYNMTDIQAALALAQMRRADSMRARR
                     EAIAGRYSEGLRGVVGVPSVADGMRHAWHLYQIAVPSRDGRPGRTALARALKDDGIGT
                     SVHFKPLHLFPWYQERLGLRTGQYRAAETCYEESLSLPIWPDMTDAEVDQVIERVKHH
                     MG"
     gene            9430..10134
                     /locus_tag="VFR25_04265"
     CDS             9430..10134
                     /locus_tag="VFR25_04265"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET9940292.1"
                     /translation="MYRRALGSLLLALTLSTPVWAETVTLTPSKDNTIFSENGGLSSG
                     VGTGLYSGEILTGPVRRALLAFDVSSIPASAVIDSVQLVLTVTQTPESTPRLFRVFRV
                     LADWGEGASNSLQGRGAPAQTNDATWTNTFFPSSLWSTQGGDFAAAASDSADVAGFGT
                     YTWRSAGLAGDVHGWTSQTSPNYGWILIGDEVTIRSVREFSSREGGAPPQLTIHYSVA
                     TPVNQTTWGKIKILYR"
     gene            10233..11051
                     /locus_tag="VFR25_04270"
     CDS             10233..11051
                     /locus_tag="VFR25_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940293.1"
                     /translation="MTRTHPLVPWLLIPLTVALIAGCSDDGDSNHEVPPATNQYQQAF
                     SGTFASSAETGAITFTVSSSTPWSSFGRRMLGPSARATLGPVTGALTFDGVTTQAVTG
                     VYDADADTIYLTVGGYALSGRYFDTSLPPHISGGVTGSHEGSFHCVMGTDTSVAIYGG
                     DWGFRGGSAVGRFSFVTKGNALFGGMILPNPQWPFLGYHFSGTISDGQPIRTITAAGR
                     GLLDSLSIGGYADTSADTAGGFWDSIPLVDEIGDGWWHVQRLRPQWSQTARSMP"
     gene            11657..13849
                     /locus_tag="VFR25_04275"
     CDS             11657..13849
                     /locus_tag="VFR25_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET9940294.1"
                     /translation="MDRGGAGAETDSRYTPPSAQSDTTSAAPVMAAPQRAPLKPEDYP
                     EFTKEIQARVPDTGPVFPFGYEIFGYAPSTFEPLASGPVDPDYPIGPGDEVIVQVWGD
                     NEFTHASVVSREATITVPDIGQVVLNGLTLAQAKRLITDRLGAVYSGIRARRPSTFVD
                     VTLGKIRTIQVFILGDVVRPGGYTISSVSTVLNALYNAGGPTARGSMRAIRIIRHNDV
                     WREVDLYSYILTGSKAEDARLQSGDVVFVPPVGKKAAVLGEVHRSAIYELKDGEGFRD
                     LLRLAGGVQSTAVIDRALVDRVVPFDQRAAMRDEDRVALDIPLKDILADSTSNPDVVD
                     RDIVQIFRVGETRKNTVSIAGTAVIRPGIYQLRPGMTISQLVSDAGGLKADAYMDRAT
                     LVRTSEDGTRSIRPFNLSEAMEKDADDDIALQKLDEITVVSIWEIQERHTVSINGSVR
                     EPGTYEYLEGMTVMDLIFRAGGLKESAYKQEAEVARIDSSTIAAKKSADVYRVAISGN
                     YGVHSPDPTFKLQRMDQVFVRQIPDWQVQRNVVVTGEVRYPGVYSLKEQNEKLSSVLF
                     RAGGLKETAYPRAAVFVRRKGNAGRLAVDVEDVAKHHKRYDLALEDGDSLNIPKQPQT
                     VKVVGEVGFPASVLFEKGKSLSYYIDQAGGYTDTSDKGRVKIVQPNGKVRPKKGLWSN
                     PSPEAGALIVVPHKPPEEKKDTLKGVATIMTILTGAVTTIFIASEATK"
     gene            13846..>14108
                     /locus_tag="VFR25_04280"
     CDS             13846..>14108
                     /locus_tag="VFR25_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940295.1"
                     /translation="MSESTGNGHFATWRGRRWLIQTILIVASAGLATAAVSLIWPKTY
                     RAEAKILPIVSSSSPSTLLGLASGSGLSDLLSGNLGTGENPILT"
BASE COUNT         2349 a         4907 c         4645 g         2207 t
ORIGIN      
        1 atcccgtgat gaacccaacc ttcgcctatg ccgtcgccgt cgggatcgcc gccgccacgc
       61 tctatggcat cgcgcgcacc ctcggccgcc gggacgccga gagcgacgag gtcgcgttcg
      121 gtccgccgtg cgagatccag gtgctctcgc gggtgcggct cggccgcggc cgcacgctgg
      181 tggtcgtcga agtggaagga aagcggctcc tgcttggatc cacgtcgtcc gaatggaccg
      241 ccctcgccga tctgggtccg gtcggagcga cggtcgccgt ctcggacgat ccgttcggcc
      301 ccatcgacgc cgagctggcg cgcgccatcc aggcctcgcg ctccaagcgg agcaacaact
      361 agccgtgcgc agcgccctcg tcactctcgc ccgtaccggc cagatcttga gcaccccgcc
      421 gtcggtccac cgggatttgg tcgctggatc gcccgacgaa acgctccaac tcgttgcggc
      481 tcaatgcact cactcccagg caccggcctt gctttgcccg ggatgcacga cgacgcgcga
      541 cgcacgcgcc gcccgcaacc cgaatccgga gtcctcccga tgagtgacat cgagaacaag
      601 tcgaagtcca cgccgaccgc gaaggcggcg cccacgatcc gcccggttgg cggatcgaag
      661 agcgcgtccc gacgcgcacc cggcggaaag tccaagcgct cgtccaagcc ggtcgtcccg
      721 ccgcctccat cccggccgcg gcgccgcacg atcgcggtca cgagcggcaa ggggggcgtg
      781 ggcaagacca acatcgtcac caatctcgcg atcgcgctcg cgcgccaggg catccgcgtc
      841 ctcgtgctcg atggcgatct cgggctcgcg aacgtggacc tgctcctcgg cgtcgcgccg
      901 cagttcgatc tccaggacgt gatcctgggc gggcgcaacg tgcgggacgt ggtcctcgaa
      961 ggacaggacg gcatccgtgt cgtgccggcg tcctcgggcg tcgaggagct cgcgaatctc
     1021 gacgagtacc ggaccgaggt gctcctccgg tccctcgccg agctcgacgg cgaagtcgac
     1081 atcatcctca tcgattcccc gtcgggcatc ggcacccacg ccacgtcgct cattcagggc
     1141 gccgatcaga tcctcgtggt cacgacgccg gagccgacgt ccttctccga cgcgtacgcc
     1201 atgatcaagg tcctcgtgcg gcgcccgctc aagtgcacgc ccgcgctcct cgtgaaccag
     1261 gccgactccg aggagagcgc gatcgccgtc gcgcgccgcg tgaagaacgt ggcgaagcgc
     1321 ttcctcaatc tcgacgtcga ctactggggc tacgtgctgg acgacgagtc cgtgccgaaa
     1381 tcggtcctgc gccaggagcc cttcctctcg acgtatccgt actcgccggc ttcgagctgc
     1441 atctaccagc tcgcccgcaa gatcatgggg cagcaaggcc ggaaccccgc tccgcacggc
     1501 ggaaacggcg gccacagcgg tcacaaagac atggccgaaa ccaccctctc gatcgtcgat
     1561 ccgtcggagg aggcgtgatc gtgcgacgcc tcgtgatccg actcaccctc atcgcgtccg
     1621 tgctcgccgg actcgccgcg accattctcg ggttcgcgac gggaacgcag ccgcacgtcg
     1681 tgatgttccg agcgctcctc gccttcacca tcgtgctcct cgcgggtgct ggcgtgggtc
     1741 tcgtcgtcat gcgcaccgcc ctgcgccggt actacgagca gggtcgccgc tccggggacg
     1801 gcaggcttca aaccaacccg gtgtcctcga accgcccggt cccgacggac cgccgcgtcc
     1861 ctgcggaccg gtagcggagg attccgatca tgcccacgcc gtccgtgctc cattcgctgg
     1921 ctcaccgcac gccgcgctcg cgcaatcgca agcagcggca gaaagaggtc gcgctcagca
     1981 agtacgcgcc cctcgtgaag tacgtcgtcg accggctcgc gctgcatctg ccgaaatcgg
     2041 tggagcggga cgacctgatc tcggcggcca tcatcggcct cttcgatgcg ctcgagaagt
     2101 acgacgcctc gaaggggacc aagttcgaaa cgtacgcgat ctggcggatc cgcggcgcca
     2161 tcctcgacga gctccgctcg ctcgactggg cctcgcggtc cattcgccgc aaggcgagga
     2221 acgtcgagga agtcgcgcgc gagctggggc agaagctggg acgcgccgcg accgaggaag
     2281 aggtggccga ggcgctcaac ctgagcccgg tcgagctttc ccggctcatg gacgaggtgc
     2341 acggcacggc gctcctctcg ctctccaagt cggtgtcgag cgacgaggac caggacttca
     2401 tccagctcga ggacatcgtc gacgatccgt cccacaagga cgcgctcgag gtgatcgaga
     2461 gcgacgaggc gcgcgaggtg ctcctcgaga cgatcgacgg ccttcccgag cagcagcgcc
     2521 tggtcgtcgc gctctactac tacgaggaga tgacgctcaa ggagatcggc gaagcgctcc
     2581 acatctccga atcgcgcgtg tcgcagatcc acacgcgcgc cgtgaagacc ctcaagggcc
     2641 gcctcggcag gctcttctga tcgtgacccc ctcccgcacc ggaacgaacg ctccccgccc
     2701 gagcgccccg cgcaacgtcg gcgcgaagcc cgcgcgcacg aacacggccg cgcccggcac
     2761 gctcgatcgc tcgtcgctct tgaagctcac cgagagcatg gtggcgctcc cgacgcttcc
     2821 gctcgtcgcg acgcggctcc ttcgcgcggt ggcgcagccg gacaccactc cggacgagat
     2881 cgcccgcatc ctctcgctcg atcccgcgct cacggcgcgc acgctccggc tcgcgaactc
     2941 cgagttctac gggtttccgc gccgggtcgg ctcggtcgat ctcgcggtcg tcgtcctggg
     3001 cgcgaacccg atccgggatc tcgcgctctc cgcgtccgtg ttccggacca tcggcgaggc
     3061 cgacgccctc atggaggacg tctggcgcca ttcgctcgcg gcgggggtag cggcgcggat
     3121 cctcgcggac cgcacgcgct accgcctcga gagcgaggcc tacgccgccg ggctgcttca
     3181 cgacatcggc accgtggtgc tgcgccagac cgatccctcc cgcttccagg cggtgatcgc
     3241 gatggtgcgg gaccagggtg aaacgatcga ggaggccgag cgcggtctct tcgggtcctg
     3301 ccacgccgag gtgggcgcgt ggctggccga gcgctggggg cttcccgccg atctggtgga
     3361 agccattgcc tgccatcacc ggccggagac ggcgagacgg aatcccgagc tcgccgctct
     3421 ggtccacgtg gccacgtcac tcgcggaacg ggccggattc gcctggcccc ggggggtcgc
     3481 gcgccgaccc atcgctcctt ccgcgttcga agtggtcgat gccgacgagc ggaagcgcga
     3541 ggccctcctc gagacgctcg tcgaagacgt ggtccgcgaa acggagcggg agcaggagct
     3601 cttcgccgaa ttccgcggat cgcaggagga ttgacgttca tgggcagtgc cgcaaccaag
     3661 tccgcacacg accgggacat cgagatccgg gttcgcttcg agcccgggct gggagccgcg
     3721 ctctacgcct cgcttcgaga cggcgaggtt ggagccgtga ccgtggccaa gaacctgggc
     3781 cgcggccgct cactcctccg ttatcacggc ttcacgctga tggcgcaggg ggctcccgac
     3841 tggaaggtgg gggattcgtt ctcggtctac gtgaaggagc tcggtccgcc gctcgttctc
     3901 gccccggtgg gcgcgaacgg acggagcgcg agccggcgcg tcgccacggt ggagtcggcc
     3961 gtggtcccgg gcgttcccca gtcgtccaac aacgcggtcg gccgctaact ccagggaggg
     4021 agccatgaag cagcgcaacg gtgcggggtc caagcggctc gtcgaggcga ccgagcggtt
     4081 catcaagtcc gagcagaagc tcctcgaagt gctcggtggg cggcggattc tcaagaagac
     4141 cgaatgcgat cacttcgcga aggacctcgc gctctccgcg gcgaagaagc gctcgtccga
     4201 cgtgttcgcg agcgccatct cgatcaaccg gaagctcatg ggcgtgctgt ccaagtcccg
     4261 cgcggcggcg gtgaagaccg tgtccgagag cgacgtgctg gcggagctcg gccgcatcgc
     4321 gcagaccgtg atggatcctg ccgacgcctt cgagcaggcg ctcgtccaga tgcgcgcggt
     4381 ggtgccgtac gagaacgcga ccctcttcct ctggagccgc gagacgcagt gcctcgagga
     4441 ggcggcgacc gtcggctcgc gcgtggatct gatcggccac gtgcgcttcg gccagggcgc
     4501 gggtttctcc tcgtgggtgg ccgagcagcg aaagccggtt ctcctgaacg accttcatcg
     4561 cgaagggggc ccggacgcgg tggcgctccg gtccttcctc tcggtcccgc tcctcgtgca
     4621 gaacgaggtg gtgggcgtga tcaacatgag ccattcccgc cccagcgcct tcgaggagga
     4681 gatggtgacg cgcgtggcgc tgttcggcct cctcatcgcc tcggtcgcga tgcgcacggt
     4741 gctccgcaag gatcgcgaac ggatggcgac gacggacgcc ctgacgtcgc tctacaacaa
     4801 gcgccacttc gaccagtcgc tcgaggtcga ggtcgggcgc gccaagcgct atggccacaa
     4861 ggtgtccgtg ctcgtgctgg acgtggaccg tccccgcgag ccgaagacga agatcggcgc
     4921 ctccgccgga gagcaggtcc tctccgacat gggacgcctc ctgaagcgct ccgcccgtac
     4981 gacggactgc atcgcgcggt acggctacga cgaattccgg atcctcctgc cgcacaccga
     5041 cgaggcccgc gcgaaggtcg cggccgaccg gatccgcggc gtggtggaga agcacgcatt
     5101 cccgcgccgc cgccgtccga cggtgcacat cgggattgcc acgtatccga cggattccgc
     5161 gctggctccg gtcacgacgc ccaatctcga gcgtgcggtc gacgccgtga tggcgccgcc
     5221 ggtcgacatc gccagcccaa gtgaagcggc cgcgagctga ggagaagatg acggaacgga
     5281 cgatgacgcc gacggcgtcc tccggcaccg acgcgccacg cgcgaggatc ctcgtcgtgg
     5341 acgacgagat ccaggtcgtc cacatcttcc gggacctgct cacgcagcag ggctatgagg
     5401 tcgaggactg cgacaacggc gacgacgcca tcctcaaggt cacgaacggc aagttcgacc
     5461 tggtcctgac cgacatcaac ctgccgggcg tggacgggct cgaggtgatc cgcgcggcca
     5521 aagccgccga caaggacacc tgcgtcatcc tgatcacggg ctacgcgtcg acgaccacgg
     5581 ccatcgacgc ccttcgtcag ggagcctacg actacatcac caagcctttc gatctctggg
     5641 aaacggccaa ggccatcgag cgcggcctcg agtcccgcaa tctgagccgg gagaacacga
     5701 ggcttttgaa gcagctcggc gatgccaacg ccgagctcca gcagcacgag gagatactca
     5761 agcggaaggt ggagcgcgcc acccaccgaa tggcgtcgct ctacgaggcc ggcaaggcga
     5821 tctcgacgag cctcagcctc cagagcacgc tcgaggtcat cgtcgcgcag gcggcgcggc
     5881 tcaccgcgtc caactcctgc ctcgtcttcc tgcacgacac ctcgacggac gagtacgccg
     5941 cggaggcggg gcttggcgtg aagcgggaga gcttccagtc gcttcgcttc ggcatgggcg
     6001 tcggctatca cggccaggtc gtccagaccg acaagccgct cctcgtcgat gagctcgaga
     6061 gcaaggtggg cgcggagccg ctcttctcga agctcaaagc ctcgagcgcc ctcatcgtgc
     6121 cgctccgctc gagcgagagc gtgctcggaa cgatcacctg cctcgatcat gagggaggct
     6181 cgttcacgac cgaggacatg gacgttctca tgatgctcgc gagccaggcc acgatcgcga
     6241 tcaccaacgc catcctcttc gagcggacga aggagctgga tcgcctcaag tccgagttcg
     6301 tggcggtcgt ctcgcacgag gtgcgcacgc ccatcacgtc gatcaaggga tcgctcgagc
     6361 tcctctcgga cgagcggttc cacaagcttc cggccccgca gaaggagctc ctctccatct
     6421 gccaggccaa caccgagcgt ctgatcagtc tcatcaacga catcctcgac ttctcgaagc
     6481 tcgagagctc caagctctcg ctcaacatcg agtcgatcga ggtggacagc gtcctctccg
     6541 aggccgtgga gaacatccga agcctggccg cgcagaagga agtggtgatc gacctcgtgg
     6601 tggacgcctc ggtcggcaag atcgaggccg accccatgcg cgtcggacag gtcgcgacga
     6661 acctgatcgg aaacgcgatc aagttctcgc ccgacaaaag ccggatcgag gtgttcgcct
     6721 cgggcgatcc ggagaagatc acggtctccg tgaaggacta cgggcgcggc atctcggccc
     6781 gcgacatcag ccggctcttc cagcgcttcg cccagctcga ctcctcgacc acgcgcaagg
     6841 cgggcggcac gggcctcggc ctcgtgatct cgaagggaat cgtcgagcag cacggcggga
     6901 acatctgggt ggagtctgcc ctcaacgagg gctccacgtt ctccttctcg ctgccgcggt
     6961 tccgggcgga cggcggctct cccacgacct agccgcgcgc ctcccgcgca tcgacgcgcg
     7021 ccagccccca gcgtagacgc gccccggcgc gggccgtact atcccctcgc gtgacctccc
     7081 aaccgaccgt actcagcgtc ttcgggacgc gccccgaggc catcaagatg gccccggtga
     7141 tccggcgctt gaagcaggag cccggactcc gctccctcgt ctgtgtcacc gcccagcatc
     7201 gcgagatgct cgaccaggtg ctccgctggt tcgagatccg ccccgatcac gatctcgatc
     7261 tcatgcggga cgggcagacc ctgaccgaga tcacgacgcg cgcgctcggc gcgctcaccc
     7321 cgtacttgga gcgcgagaag ccgtcgctcc tcctcgtgca gggggacacc acgacgacga
     7381 tggccgcctc gctggccgcg ttctacgctc gcatcccggt cgcccatgtc gaggccggcc
     7441 ttcggacggg tgacaacatg tacccgtatc ccgaggaggt gaaccggcgc atcaccacgg
     7501 tcatcgcatc gcggcacttc gcgccgaccg agggcgcccg cgcgaatctg ctgcgcgagg
     7561 gcgtggatcc ggcgtcgatc ctcgtgacgg ggaacacggt catcgacgcg ctcctcgagg
     7621 tcgtgaagca ggagcccaag cgcacgccca agctcccgct tcggggcaag cggctcgtcc
     7681 tggtcactgc gcatcgccgc gaaaacttcg gcgcgccgct cctcgagatc acgggggcgc
     7741 ttcgcgatct ggcggagcgt tatcctgatc tggatctggt ctatccggtg catcggaatc
     7801 cgaacgtgga cgggcccgtg cggaaggcgc tcgggggcgt ggcgggcgtc catctcatcg
     7861 atccgctcga gtacaagccg ttcgcggatc tcatggcggc gtcgacgttg atcctcaccg
     7921 actccggagg aattcaggag gaggcgccgt ccctgggaaa gccggtgctc gtgctgcgcg
     7981 acgagaccga gcgccccgag gcggtggagg cgggggtggt gaagctggtg ggtccgcatc
     8041 gcgatgcgat cgtgcgcgcg gcttcggagc tcttggacga ccctgcgcgc tatcgcgcga
     8101 tggcggcggc ggtgaatccc tacggcgacg gccacgccgc ggagcgcatc gtgaaggtca
     8161 tccgggagat gctcggtgta ggctgaggga cgcctacccc atgtggtgct tcacccgctc
     8221 gatcacctga tccacctcgg cgtcggtcat gtccggccag atggggagcg agaggctctc
     8281 ctcgtagcag gtttcggccg cgcgatactg gcccgtccga agacccaggc gctcctgata
     8341 ccacgggaag agatggagcg gcttgaagtg gacgctcgtg ccgattccgt cgtccttcaa
     8401 cgcgcgcgcc agagccgtgc gtcccggccg tccgtcgcgc gacggaaccg cgatctggta
     8461 caggtgccac gcgtgccgca ttccgtccgc caccgagggc acgcccacca cgccacgcaa
     8521 tccctctgaa tagcgcccgg cgatcgcttc gcggcgcgcc ctcatggaat ccgcgcggcg
     8581 catctgcgcc aaggcgagcg ccgcctggat gtcggtcatg ttgtacttgt agccgagctc
     8641 ggtgacgtcg tagtcccacc acccgccttc gtcgtaacgg ctccacgccg ccgccgagag
     8701 tccgtggagc gagaggcgtc gcatccgctc ggcccagtcg gcgtgcgcgg tgaccgccat
     8761 gccgccctct cccgccgtca cgcccttcgt cgcgtagaac gagaagcagg tgatgtccgc
     8821 ctgggatccc accggcacgc cccagatctg tgcggtgagc gagtgggcgg cgtcctcgat
     8881 catggggagc ttgtgctcgt gcgcgatggc cgcgatctcc gcggcgcggc agggatggcc
     8941 ggccatcgaa accgatacga tggcgcgcgt tcgcggcgtg atggcggcgg ggaggagcct
     9001 cggatcgagg ttcttcgtgt cccgctccac gtccacgaac acgggcttgg cgccgaggta
     9061 gaggatggtc tcgccggtcg agacgaacgt gtacggcgtt gtcacgactt cgtccccttc
     9121 gcccacgccg gccgcgatga gggccaggtg gagcgcggcg gtgcaggacg agaccgcgac
     9181 tccatggagc ggccctcgcg cgaccccgcg gccgcccgac gcgatcgcct ccgccggggg
     9241 cgtcacgtag gccacgaccg cggcctcgag ctccttcacg cgggggccgg tcgtgagcca
     9301 gccggagcga aggacggcag ccaccgcgtc cagctcgcct tggccgatcg aggggcgatg
     9361 gaaggggatc gaggccatgg gcccgcacgc tagcacaggc aaggccacct gcggtacgct
     9421 acccgtccca tgtacagacg cgccctggga tccctgctcc tcgcattgac tctctcgacg
     9481 cccgtatggg cggagacggt cacgctcacc ccctcgaagg acaacacgat cttcagcgag
     9541 aacggcggtt tgagcagcgg ggtgggaacc ggcctctatt ccggagaaat cctcaccggt
     9601 cccgtccggc gcgcgctcct cgcgttcgac gtgtcctcga taccagccag cgccgtcatc
     9661 gacagcgtcc agctcgtgct gacggtgacg cagactcctg aatcgacgcc gcggctcttt
     9721 cgcgtcttcc gggtccttgc cgattggggc gagggagcct cgaattccct gcagggaagg
     9781 ggggcgccgg cgcagaccaa cgacgccacg tggaccaaca cgttctttcc gagctcgctc
     9841 tggtcgacgc agggcggtga cttcgcggcg gcagccagcg attccgcgga tgtcgcgggc
     9901 ttcggaacct acacctggcg ttcggccggg cttgccggcg acgtccacgg atggacctcg
     9961 cagacgtctc cgaactacgg ctggattctc atcggagacg aggtcaccat caggagtgtc
    10021 cgcgagttca gctcccggga aggcggagcg cctccccagc tcacgatcca ctactcggtc
    10081 gcgacaccgg tgaaccagac cacctggggc aagatcaaga tcctgtaccg ctgaagcatc
    10141 tcggaactcc aagttcggat tgaccttacc cgccggtctg ttattctgaa gtccctcccc
    10201 ttgagaccgg ttcgagttga cagcgagggc tcatgacgcg cacgcatccc ctcgtcccct
    10261 ggctactgat ccccctgacg gtcgcactca tcgccggttg cagcgacgat ggcgacagca
    10321 atcacgaagt cccgccggca accaaccaat atcagcaggc ctttagcggc acgttcgcga
    10381 gctcggccga gacgggcgcg atcacgttca ccgtcagttc gtcgactccg tggtcctcgt
    10441 tcggccgcag gatgctcggt ccgtccgcgc gcgccacgct gggcccggtc accggtgccc
    10501 tcaccttcga cggcgtgacc acgcaggccg tgaccggcgt ctacgacgcg gatgccgaca
    10561 cgatctatct cacggtcggc ggctacgccc tcagtggccg gtatttcgac acgtccctcc
    10621 cgcctcacat ctcgggcggg gtcacgggat cccacgaagg ctcgttccat tgcgtgatgg
    10681 ggaccgatac gtccgtcgcc atctacggcg gcgattgggg ctttcgcgga ggatccgcag
    10741 tcggtcgatt cagcttcgtc acgaaaggga acgcactctt cggcggcatg atccttccca
    10801 atccgcagtg gcccttcctc ggctatcact tctccggaac gatttccgat ggccagccca
    10861 tccggacgat caccgccgcg gggcggggac tcctggactc gctctcgatc ggcggctacg
    10921 ccgacacgtc ggccgacacc gcgggcgggt tctgggacag catcccgctc gtcgacgaga
    10981 tcggggacgg ctggtggcac gtccagcggc tgcgtcccca gtggagccag accgcccgct
    11041 ccatgccctg acctgggtgg actctgccct cccggaatct cgacctcggc ggcaatgccg
    11101 actcctcggc cgacaccgcc ggcggctggt gggaaagcat cccccccgat agcggcggtt
    11161 ggtggcgcgt gaaccggtac gtcgccaagc ccaccgcgga cgtcaccccc taggcggccg
    11221 ggcgtctccg aaatctttct gaccggcaaa attccttgcg ccattccgcg gccgcgtggt
    11281 acctatgcgg tgggatgcgg ggtgcggatg aggaagcgcc ggactctgct ccaacgccag
    11341 cgcgaatcgt ccacaccacg gatggagtcg aacgacgaag ctgcgggcca caccgctcca
    11401 ttcccgcgcc ctagcgctct ggacctcgct ttccctcgtt ctgctctcgc tctccttcac
    11461 acacgcctac gcccagtcca catctgagct gaaacagaag gccgacgagg ccggaatcca
    11521 ttcggaggac gacgccatcc gtcgcgccaa ggcgtcgggg ctcacgcgcg accagatcct
    11581 ccaggcgctg aaggaagcgg gctacaccga gaccgaggtc cgcgaagtcc tctcgaacga
    11641 agcgccgggc agtggcgtgg atcgaggcgg cgcaggggcc gagaccgact cccgctacac
    11701 gccgccgagc gcgcagtccg ataccaccag cgcggctccc gtgatggcgg cgccccagcg
    11761 cgcgccgctc aagcccgagg actatcccga gttcacgaaa gagatccagg ctcgcgtgcc
    11821 cgatacgggg cctgtgttcc cgttcggcta cgagatcttc ggctacgcgc cgtcgacgtt
    11881 cgagccgctc gcgtccggtc ccgtcgatcc cgactatccc atcggcccgg gggacgaggt
    11941 catcgtccag gtctgggggg acaacgagtt cacgcacgcg tcggtcgtga gccgcgaggc
    12001 cacgatcacc gtgccggata tcggccaggt cgtgctgaac gggctcacgc tcgcgcaggc
    12061 gaagcggctg atcacggacc gtctcggcgc tgtctactcg ggtattcgcg cgcggcgccc
    12121 gagcacgttc gtcgacgtca cgctcgggaa aatccggacg atccaggtct tcattctcgg
    12181 ggacgtggtg cgtccgggcg gctacaccat ctcctcggtc tcgaccgtcc tcaacgcgct
    12241 gtacaacgcg ggcggtccga cggcgcgcgg ctccatgcgt gcgatccgaa tcatccgcca
    12301 caatgacgtc tggcgcgagg tcgacctcta cagctacatc ctcaccggga gcaaggccga
    12361 ggacgcgcgc ctccagagcg gcgacgtggt gttcgtgccg cccgtgggga agaaggcggc
    12421 ggtgctgggc gaggtgcacc gcagcgccat ctacgagctc aaggacggcg agggattccg
    12481 cgatctcctg aggctcgcgg gcggggtcca gtcgaccgcc gtgatcgacc gcgcgctcgt
    12541 ggatcgcgtc gtgcccttcg accagcgcgc ggccatgcgc gacgaggacc gggtcgcgct
    12601 cgacatcccg ctcaaggaca tcctcgcgga ttcgacgagc aaccccgacg tcgtggaccg
    12661 ggacatcgtc cagatcttcc gggtggggga gacgcgaaag aacaccgtgt ccatcgcggg
    12721 gacggccgtc attcgcccag ggatctacca gctccgtccc gggatgacca tctcccagct
    12781 cgtgtcggac gccggcgggt tgaaggccga cgcctacatg gaccgggcga ccctcgtccg
    12841 cacctccgag gacgggaccc ggagcatccg gccgttcaat ctcagcgaag cgatggagaa
    12901 ggacgcggac gacgacattg cgctccagaa gctcgacgag atcacggtgg tctcgatctg
    12961 ggagatccag gagcggcaca cggtctcgat caacgggagc gtgcgcgagc ccggcaccta
    13021 cgagtacctg gaaggcatga cggtcatgga cctcatcttc cgcgccggcg ggctcaagga
    13081 gtcggcgtac aagcaggagg cggaggtcgc gcgcatcgac tcgagcacga tcgcggcgaa
    13141 gaagtcggcc gatgtctacc gcgtggccat ttcggggaac tacggcgtcc actcgcccga
    13201 tcccacgttc aagctccagc ggatggatca ggtgttcgtg cggcagattc cggactggca
    13261 ggtgcagcgg aacgtggtcg tcacgggcga ggtgcggtat ccaggcgtct actccctgaa
    13321 ggagcagaac gaaaagctct cctcggtcct gttccgcgcc ggcggtttga aggagacggc
    13381 gtatccgcgc gcggctgtct tcgtgcgtcg gaaagggaac gccgggcgtc tcgcggtgga
    13441 cgtggaggac gtggcgaagc atcacaagcg ctacgacctc gcgctcgagg atggcgacag
    13501 cctgaacatc ccgaagcagc cccagaccgt gaaggtggtc ggggaagtcg gcttccccgc
    13561 gagcgtgctg ttcgagaagg gaaagagcct ctcctactac atcgaccagg caggcgggta
    13621 cacggacacg tcggataagg ggcgggtgaa gatcgtgcag ccgaacggaa aggtgcggcc
    13681 gaagaagggt ctgtggtcga atccctcgcc cgaggcgggc gcgctcatcg tggtgccgca
    13741 caagccgccg gaggagaaga aggacacgct gaagggcgtg gcgaccatca tgacgatcct
    13801 gaccggggcc gtcacgacga tcttcatcgc gagcgaggcg acgaagtgag cgagtccacc
    13861 ggcaacggcc atttcgctac ctggcggggc cggaggtggt tgatccaaac gattctgatc
    13921 gtcgcctcgg caggcctcgc aaccgccgcc gtctccttga tctggcccaa gacctaccgc
    13981 gccgaagcga agatcctgcc gatcgtctcg agctcatcgc cctcgaccct gctggggctc
    14041 gcgtccggat cggggctgag cgacctgctg tccgggaatc tcggcaccgg cgaaaatcct
    14101 atcctcac
//
LOCUS       DASTKC010000102         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_312508, whole genome shotgun sequence.
ACCESSION   DASTKC010000102 DASTKC010000000
VERSION     DASTKC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312508"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFR25_04285"
     CDS             <1..263
                     /locus_tag="VFR25_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940296.1"
                     /translation="IAGRPDQDARTAIRADHVPSVRTGVIAAIVPTGRIAVTADRGLT
                     AATEDRDRIAATAGPGPTGATRATVLTATGAPAPRRPPTVAA"
     gene            398..1381
                     /locus_tag="VFR25_04290"
     CDS             398..1381
                     /locus_tag="VFR25_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9940297.1"
                     /translation="MRALLSGYQSTAVLLAAHSLGVFRHLHQRPQNLDNLTRAAGADR
                     RGMEALLEALVGLGVVHRHGPTFVLPRDFAPYLVPGVDGDATGMIDATQDYYMAWMEL
                     ARAVREGTPRHRLSSDALIAGDPERARRYIRSVHTGGREAARRLTELAPALPGSKLLD
                     VAGGSGIYAAEYARRTPDLQATLFELPPTIETARDILRAEGLEDAIEYVPGDYRTDPL
                     PGPVDTVLLSNVLQTESEENAILLLRKAREALNPGGTILIHGWMPEENGTKSQTMGMA
                     PLLFFMYFDQGRAWSLERVSEWLAQEGFGVRSSRPLGAPFQTRLLIATRLE"
     gene            1384..2274
                     /locus_tag="VFR25_04295"
     CDS             1384..2274
                     /locus_tag="VFR25_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9940298.1"
                     /translation="MPELPFLEILAENLASSVSGRRIEAFDVRQPALLQRPEPRPESF
                     IGEFVSFPRRVGKYLVLETESCRVLVFHLMRLGRITVAPAASDRRPARGARAAVGAPA
                     KNVSARIRFDDGSELRLIEHGTEKKARLWLADDIDSVPELGRLGPDPARGELTPDRFL
                     QAVRAESRQLKTLLTDPAAIAGIGNGLSDEILYEARLSPMKRSDQLDDAEAARLHAAV
                     LSVVERQVALLRESADGALPQKEPVEHYVVHDHKGEPCPRCGTAIAWVSYAERDTFYC
                     PGCQTGGVPLKDRRLSKLLK"
     gene            2344..2736
                     /locus_tag="VFR25_04300"
     CDS             2344..2736
                     /locus_tag="VFR25_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940299.1"
                     /translation="MSLRRVISIVLLGALLAMQAAPARAGRMLCKMKVPARTEACSRC
                     DAPRASEANGSLRATDCCRVTPRSASEATPIAPAPRAASSHDPLPSMMQSASFTFMAL
                     DADHQATWIPSGGPPGSERLSRSTILRN"
BASE COUNT          427 a         1098 c         1004 g          468 t
ORIGIN      
        1 tgatcgcggg ccgtccggac caggacgccc gtaccgcgat tcgcgcggac cacgtcccga
       61 gcgtccgtac cggagtgatc gcggcgatcg tcccgaccgg ccgtatcgcg gtgaccgcgg
      121 accgaggcct gaccgcggcg accgaggacc gagaccggat cgcggcgacc gcgggccccg
      181 gcccgaccgg ggccaccagg gcgaccgtcc ttaccgcgac cggagcgccg gccccgcgcc
      241 gtccaccgac cgtcgcggca tagcaccgca ggcgccgggc gccgaccggg ggccgcacca
      301 tccgagacgc gaccgtccgt atcgcaccgg cgtccagagc gcgggggaag gcccaccgcg
      361 cggcgtcccg gttcccgagg aggccccact gggtcgcatc cgcgcccttc tctcgggata
      421 ccaatcgacg gcggtcctgc tggccgcgca ttcgctcggt gtgttccgcc acctccatca
      481 acgtccacag aatctcgaca atctgacgcg cgccgcgggc gcggaccggc gcggcatgga
      541 ggcgctcctc gaagcgctgg tgggactcgg cgtcgttcat cgccacgggc ccaccttcgt
      601 tcttccgcgc gacttcgctc cgtatctcgt gccgggcgtc gacggcgacg cgaccggcat
      661 gatcgacgca actcaggact actacatggc gtggatggag ctcgcgcgcg ccgtgcgcga
      721 aggcacgccc cggcatcgcc tctcgagcga tgcgctcatt gcgggcgatc ccgagcgtgc
      781 gcggcgctac attcgctccg tccacacggg aggacgcgag gccgcgcggc gtctgaccga
      841 gctggccccg gcgctccctg gatcgaagct cctcgacgtg gcgggcgggt ccggcatcta
      901 cgcggccgag tacgctcgtc gcacccccga tctccaggcg acgctcttcg agcttccgcc
      961 cacgatcgag accgcgcgcg acatccttcg cgccgaggga ctcgaggacg cgatcgagta
     1021 cgtgcccggc gactaccgca cggatccgct ccccggtccc gtggacacgg ttctcctctc
     1081 gaacgtcctc cagaccgaga gcgaggagaa cgcgatcctg ctcctacgca aggcgaggga
     1141 agccctcaac cccggcggca cgattctcat ccatggatgg atgcccgaag agaacggcac
     1201 caagagccag accatgggga tggcgcccct cctgttcttc atgtacttcg atcaggggcg
     1261 ggcgtggagc ctggagcgcg tgtccgagtg gctggcccag gagggttttg gtgtgcgctc
     1321 gagccgcccg ctcggggctc cgttccagac gcggctcctg atcgccacgc gcctcgagta
     1381 gccgtgcccg agcttccgtt cctcgagatc ctcgccgaga acctggcgtc ctcggtctcg
     1441 gggcggagga tcgaggcgtt cgacgtgcgc cagcccgcgc tcctccagcg cccggagcca
     1501 aggcccgagt ccttcattgg cgagttcgtg agcttccccc ggcgtgtggg gaagtatctc
     1561 gtgctcgaga cggaatcctg ccgtgtgctc gtcttccacc tcatgcggct cggacggatc
     1621 accgtggcgc ccgcggcatc ggaccgtcgt ccggcgcgcg gcgcgcgggc tgcggtgggt
     1681 gcgccggcga agaacgtctc cgcgcggatc cgcttcgacg acgggagcga gcttcgcctg
     1741 atcgagcacg gaacggagaa gaaggcgcgg ctctggctgg ccgatgacat cgactccgtc
     1801 ccggagctgg gccgtcttgg acccgatccc gcccgaggcg agctgacgcc cgaccgcttt
     1861 ctgcaggcgg tgcgcgcgga gtcgcgccag ctgaagacgc tcctcacgga tcccgccgcg
     1921 atcgccggga tcgggaacgg cctctccgac gagatcctct acgaggcgag gctctcgccc
     1981 atgaagcgct ccgatcagct cgacgacgcg gaagccgcac gcctccacgc ggccgtgctc
     2041 tcggtggtcg agcgtcaggt cgcgctcttg cgcgagagcg cggacggagc cctgccgcaa
     2101 aaggaaccgg tggagcatta cgtggttcac gatcacaagg gggagccttg tccccgttgc
     2161 gggaccgcga tcgcctgggt ctcgtacgcg gaacgggaca ccttctactg ccccggctgc
     2221 cagacggggg gcgtgccgct caaagatagg cggctgtcga agttgttgaa gtagacggcc
     2281 ttggctgccg atactcccag ggacgtcgtc acgaaagccg cttccccgtg tactatgggt
     2341 gacatgagcc tacgtcgcgt gatctcgatc gttcttctgg gcgcgctcct cgccatgcag
     2401 gcggcaccgg cccgcgccgg gcgaatgctc tgcaagatga aggttccggc gcgcacggag
     2461 gcttgctcgc gctgcgacgc tccgagggcg agcgaggcga acggatcgct tcgggccaca
     2521 gactgctgcc gcgtcacgcc ccggtccgcg tcggaggcga caccgatcgc cccggcaccg
     2581 cgtgccgctt cgagtcacga cccgctcccc tccatgatgc agtcggcgtc gttcacgttc
     2641 atggcgctcg acgcggatca ccaggccacg tggattcctt cgggcgggcc gcccggctcg
     2701 gaacgcctct cccgcagcac cattctccga aactgatcct cacctccctc tagccgttcg
     2761 tctccggccc ccggccgatc gtcgccggcg agccacgatc gtcacgcgct cctcgagcgc
     2821 cgtcctgccc acgggaggtg ggttccatca tggctgcacc cgttcttcgt atcgcttgcg
     2881 tcgcgctcgc cgcggcagcc ggctggtcgg ttctgccggt ccgctccccg ccggcatggg
     2941 ccgccggctc aggaaccgcc cagggtccgg ccgcgttcga cacgcttctt gtcgctc
//
LOCUS       DASTKC010000103         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1494935, whole genome shotgun sequence.
ACCESSION   DASTKC010000103 DASTKC010000000
VERSION     DASTKC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494935"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFR25_04305"
     CDS             complement(<1..321)
                     /locus_tag="VFR25_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940300.1"
                     /translation="MSARMRPMRGLVVALGIGLALAVASHAAAKPKPASDKASKQAET
                     PGPKSAIPNVPELSKPGQPQPQDPQAVDVYRAILRSSASRAAADTLGDGMYHAALQAF
                     YDGQI"
     gene            complement(318..3671)
                     /locus_tag="VFR25_04310"
     CDS             complement(318..3671)
                     /locus_tag="VFR25_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940301.1"
                     /translation="MNPSYQALLERLRAARRIILLRAIERAGIATVVGLLAVALLALA
                     IALVAPLYRAEYAAIRNALLGAAVLFVLAAVVRVWSTKAALSQAAIEAGRLGGDREDE
                     LLAALELSQGAEGGDAWTSSSLREAAVHAAAERAQGLPIERLRRWDQRGRWLAASGGV
                     LLLLAVTGVLGGSRTPMVIQRIARPASAPIAPIKIRVEPGNREIEGGETVSIRAYVAG
                     TPRHPKLLMRSADGWKDASFDDAPSAEGARSGEHVYAATLRNVKEDVTYKVKIADQET
                     PVFALSVRDLPRATGYRIRYDYPAYTGLKSEEAQAITADLAAPRGTRATIDVGTNRAV
                     DDAAILFDGGGQIAGSGGDHGAEITVPIRSDGRFSIRLTDRRGRRAVLGPFELRAIPD
                     RPPTVTVLAPGPVDDVGHDMGTTILAGATDDHGVRKILLRYKVREEAVKVETLHEEKA
                     GARELAIRYGWALGSYGLLPGDEIEYQVGAVDGNAVDGPQTTWSDTRRLRFPSATEIL
                     ASMDRERSESIDTLEDVLKNVRDLQQKSEDLSRDVGRTKELTWEQKQELQKNLEGQQQ
                     AKEQLDKVAEKLSQDAEKLSQSPLRSELVQKIEELHQLLSQIKDQSLLKSMQRLQEAM
                     KKMTPQEMERALQNFQLSQDEVIKNLERTIEMLKQLKTEEMLEAASQRAAEMERRQLA
                     VNDSLSRAQGKDQKSTLNQLSNSQKQLSDMSKEQSAALDSIASQMKPDDAETAEQAKQ
                     LAEQYGEKGAQQDMQQMSGSMEAGEQDQAQQQAKQLSKNLEQMRKSVDKMREGFSQKK
                     KNELAKKMEDAAQDLMDIASVQEKLLADDQSSMNDRAETQKGLQETTEGATERIGQMA
                     KQSLFITPDIAQSLGRALTNQSNAVGRYSNQDLAGGLNSSKEATISLNQAATGLLRQK
                     ESMQGAKSSTGMQEAMESLQSLAGQQQGVNEQTMGMMPGQQDGMGSGDQGQRLQEGAG
                     QALGRMAAEQEAIRKGLEETMQKMNQSGGSTLGNMGDVAEEMKKVEQDLRSGRLEADT
                     VERQQKILSRLLDAPRSVEKRDYSRKRTSRPGVDVVRSSPEALSGDLLKSKPSLAALL
                     ARGSRDPIAPRYRATVDEYFQSILEGKAR"
     gene            complement(3671..4399)
                     /locus_tag="VFR25_04315"
     CDS             complement(3671..4399)
                     /locus_tag="VFR25_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET9940302.1"
                     /translation="MAPFGTANRSVAALWAGSLAALLLSLGPTHASGAPGSGLTQDVS
                     GGFTLARLKYTGGGDWYSDESSLKNLLRALRARGDIRVGQDKEAVVTATDPELWNYPL
                     LFMTGHGNVKMSDEELRSMRRYLDQGGFLWADDNFGLDKSFRELMRRLYPETPLVELP
                     FSHELFRSPNAFPNGAPKIHEHDGGPARVFAIVKDGRVRVLYTFDCDIGNGIEDEGVH
                     DDPPDKRAAAMRFAVNVATYAVTN"
     gene            complement(4504..>5097)
                     /locus_tag="VFR25_04320"
     CDS             complement(4504..>5097)
                     /locus_tag="VFR25_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HET9940303.1"
                     /translation="DGCILTEASEGRLITATKGFAWIEARTEGRAAHGSRWDLGVSAI
                     ARMGRIVAALDDLDRRTLRQRTHPLVGPASLHCAKIDGGVGLSTYAPACTLVVERRTL
                     PGETPESALEEIRSTIAGAGEEANLSIVFARPPLTCDPKAKVARAAREAIQAVHGEPA
                     EEAGVPYWMDAAVFGQAGTPGAQPPSRLPEPPVPADV"
BASE COUNT          691 a         1808 c         1666 g          932 t
ORIGIN      
        1 gatctgcccg tcgtagaacg cctggagcgc cgcgtgatac atcccgtccc cgagcgtatc
       61 cgccgccgcg cgcgacgccg acgaccggag gattgcccga tagacgtcga ccgcctgcgg
      121 atcctgaggc tgcggctgcc ccggcttcga gagctccggc acgttcggaa tcgcactctt
      181 gggcccaggc gtctccgcct gcttcgaggc cttgtcgctc gccggcttgg gcttcgctgc
      241 cgcgtgtgac gcgaccgcaa gtgccaatcc gattccgaga gccacgacga gccctcgcat
      301 gggacgcata cgagcgctca tcgcgccttc ccctcgagaa tcgactggaa gtactcatcg
      361 acggtagcgc gataccgcgg cgcgatcgga tcccggctgc cgcgcgcgag cagcgccgca
      421 agcgatggct tggacttcaa gagatcgccc gacagcgctt ccggcgacga tcgaacgacg
      481 tccacgcccg gacgcgacgt gcgcttccgc gagtagtccc gcttctccac gctccgcgga
      541 gcatcgagga gccggctcag gatcttctgc tggcgctcga ccgtgtcggc ctcgaggcgc
      601 ccggaccgca ggtcctgctc caccttcttc atttcctcgg cgacgtcgcc catgttgccg
      661 agcgtgctcc cgccgctctg gttcatcttc tgcatcgttt cttccaggcc cttgcggatg
      721 gcttcctgct cggccgccat gcgcccgagc gcctggcccg cgccttcttg gagcctctgt
      781 ccctgatccc ctgaccccat gccgtcctgc tggccgggca tcatccccat ggtctgctcg
      841 ttgactccct gctgctggcc cgcgagcgac tgcaggctct ccatcgcctc ctgcatgccg
      901 gtcgaggatt tcgctccctg catggattcc ttctgccgga ggagtcccgt cgcagcctga
      961 ttgagcgaga tcgtcgcctc cttggaggaa ttgagtccgc ccgcgagatc ctgattggag
     1021 tagcggccga ccgcgttcga ttgattcgtg agcgcgcggc cgagcgactg cgcgatgtcc
     1081 ggcgtgatga agagagactg cttcgccatc tggccgatgc gctcggtggc cccttccgtg
     1141 gtctcctgga gccccttctg cgtctccgcg cgatcgttca tgctgctctg gtcgtcggcg
     1201 aggagcttct cctgtacgga cgcgatgtcc atcaggtcct gcgcggcatc ctccatcttc
     1261 ttggcgagct cgttcttctt cttctggctg aagccctcgc gcatcttgtc gacggacttc
     1321 cgcatctgct ccaggttctt cgagagctgc ttcgcctgct gctgcgcctg gtcctgctcc
     1381 ccggcttcca tcgagccgct catctgctgc atgtcctgct gcgcaccctt ctcgccgtac
     1441 tgctcggcga gctgcttcgc ctgctcggcc gtctcggcgt cgtcgggctt catctgcgac
     1501 gcgatcgagt cgagcgcggc cgactgctcc ttcgacatgt cggagagctg cttctggctg
     1561 ttggagagct ggttcagcgt gctcttctga tctttcccct gcgcgcgtga gagcgagtcg
     1621 ttcaccgcga gctgccgccg ctccatctcc gcagcgcgct gcgacgcggc ctcgagcatc
     1681 tcctccgtct tgagctgctt cagcatctcg atggtgcgct cgaggttctt gatgacctcg
     1741 tcctgcgaga gctggaagtt ctggagcgcg cgctccatct cctggggcgt catcttcttc
     1801 atcgcttcct ggagcctctg catcgacttg aggagcgact gatccttgat ctggctcagg
     1861 agctgatgga gctcctcgat cttctggacg agctcgctcc ggagcggcga ctgcgagagc
     1921 ttctccgcgt cctgagacag cttctcggcc accttgtcga gctgctcctt cgcctgctgc
     1981 tgcccttcga ggttcttctg gagctcctgc ttctgctccc aggtcagctc cttcgtgcgt
     2041 ccgacgtcgc gcgagaggtc ctcggacttc tgctggaggt cgcgcacgtt cttgagcacg
     2101 tcctcgagcg tgtcgatgga ctcgctccgc tcccggtcca tgctcgcgag gatctcggtc
     2161 gcggacggga acctgagccg ccgggtgtcc gaccacgtcg tctgcggtcc gtcgaccgcg
     2221 ttcccgtcga ccgcgcccac ctggtactcg atctcatctc cagggagaag accataggac
     2281 ccgagcgccc agccgtagcg gatcgcgagc tcgcgcgccc ccgccttctc ctcgtggagc
     2341 gtctccacct tcaccgcttc ctcgcgaacc ttgtagcgga ggaggatctt ccgcacgcca
     2401 tggtcgtccg tggcgcccgc gaggatcgtc gtgcccatgt cgtgccccac gtcgtccacg
     2461 gggcccggcg cgagcacggt caccgtcggc ggccgatcgg gaatggcgcg gagctcgaac
     2521 gggccgagga ccgcgcggcg tccccgccgg tcggtgagtc ggatggagaa ccggccgtcg
     2581 ctccggatgg gaacggtgat ctccgccccg tgatcgcctc ccgatcccgc gatctggccg
     2641 cccccgtcga agaggatcgc cgcgtcgtcc accgcgcggt tcgtcccgac gtcgatcgtg
     2701 gcgcgcgtgc cgcgcggcgc cgcgaggtcg gccgtgatgg cctgggcttc ctcggacttg
     2761 agccctgtgt aggccggata gtcgtagcgg atgcgatagc ccgtcgcgcg cggaaggtcg
     2821 cgcacgctca aggcgaagac gggcgtctcc tgatcggcga tcttgacctt gtacgtgacg
     2881 tcttccttca cgttccgaag cgtcgcggcg tagacgtgct cgcccgagcg cgccccctcg
     2941 gcgctgggcg catcgtcgaa cgacgcgtcc ttccacccgt cggccgaccg catgaggagc
     3001 ttgggatgac gcggcgtgcc cgcgacgtac gcgcggatgg agacggtctc gccgccttcg
     3061 atctcgcggt ttccgggctc gacgcgaatc ttgatcgggg cgatcggggc gctggccggc
     3121 cgcgcgatgc gctggatcac catgggagtc cgcgatcccc cgaggactcc cgtcaccgcg
     3181 aggaggagga gcacgccgcc gctcgccgcg agccagcgcc cacgctgatc ccagcggcgg
     3241 agccgctcga tggggagccc ttgcgcgcgc tccgccgcgg cgtgcactgc ggcctcgcgg
     3301 agcgacgacg aggtccacgc atcgccgcct tccgctccct gcgacagctc gagcgcggcc
     3361 aagagctcgt cctcgcggtc gccgcccagg cgtcccgcct cgatcgcggc ctgggagagc
     3421 gcagccttcg tgctccacac ccgcacgacc gccgcgagca cgaagaggac cgcggcgccc
     3481 aggagcgcgt tccggatcgc cgcgtactcc gcgcggtaga gcggcgccac gagcgcgatc
     3541 gcgagcgcga ggagcgccac ggcgaggagc ccgacgaccg tcgcgatgcc cgcgcgctcg
     3601 atggcgcgga gcaggatgat gcggcgcgcc gcacggagcc gctcgaggag tgcctggtac
     3661 gagggattca tcagttggtc accgcgtagg tcgccacgtt gacggcgaac cgcatcgcgg
     3721 ccgcgcgctt gtccggggga tcgtcgtgaa cgccttcgtc ctcgatgccg tttccgatgt
     3781 cgcagtcgaa ggtgtagagc acgcggactc ggccgtcctt gacgatggcg aagacccgcg
     3841 cgggaccgcc gtcgtgctcg tggatcttgg gggcgccgtt cgggaacgcg ttgggagacc
     3901 ggaacagctc gtggctgaac ggcagctcga cgagcggggt ttccggatag agacggcgca
     3961 tcagctcgcg gaatgattta tcgaggccga agttgtcgtc ggcccagaga aatccaccct
     4021 gatcgagata gcgccgcatc gatcgaagct cctcgtcgct catcttcacg ttgccgtgcc
     4081 ccgtcatgaa caggagcgga tagttccaga gctcgggatc cgtcgccgtc accaccgctt
     4141 ccttgtcctg cccgacccgg atgtcgcccc gcgccctgag cgcgcgaagc aaattcttca
     4201 gcgagctctc atcggagtac cagtctccgc cccccgtgta tttcagcctg gcgagcgtga
     4261 agccccccga gacgtcctgg gtgagcccgg agcccggggc accggacgcg tgcgtcggtc
     4321 ccagactgag caagagtgcg gccagactcc cggcccaaag cgcggcgacc gaccgatttg
     4381 ccgttccgaa aggcgccatt cctctcctgg gaattgagac gctgcctggc gccggggggt
     4441 tggggctcca ggcttcgaat ctaaggatag cagaggggaa aacgccgaac cccgccccgc
     4501 tagctaaaca tcggccggaa cgggaggttc cgggagccgg gatggcggtt gagcgccggg
     4561 ggtgcccgcc tgcccgaaaa cggccgcgtc catccagtac ggtacgccgg cctcctccgc
     4621 gggctcgcca tggaccgcct ggatcgcctc gcgcgccgcg cgggcgacct tggccttggg
     4681 atcgcaggtc agagggggcc gggcgaagac gatcgacagg ttcgcctcct cgccggcgcc
     4741 cgcgatggtc gaccggatct cctcgagcgc gctttcgggg gtctccccgg gaagcgtgcg
     4801 gcgctccacg acgagcgtgc acgcgggagc gtaggtcgaa agccccacgc cgccgtcgat
     4861 tttggcgcag tggagcgagg cgggacccac gagcggatgg gtcctctggc ggagggtgcg
     4921 gcgatcgagg tcgtcgagcg cggccacgat ccgccccatc cgcgcgatcg cactcacgcc
     4981 gagatcccac cggctgccgt gcgccgcgcg cccctccgta cgcgcttcga tccacgcgaa
     5041 tcccttggtc gcggtgatca ggcgcccctc gctcgcttcg gtcaggatgc aaccatc
//
LOCUS       DASTKC010000104        10383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_550036, whole genome shotgun sequence.
ACCESSION   DASTKC010000104 DASTKC010000000
VERSION     DASTKC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550036"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1266
                     /locus_tag="VFR25_04325"
     CDS             88..1266
                     /locus_tag="VFR25_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9940304.1"
                     /translation="MSDEREYWPGPAGMMRTTILVITLLAVVWLSFLIFSPFLLAFAT
                     AAPIALLLFPTQERLAAGMGQRRSLAAILLVIWIALLVAIPLAAVLSLVGSQAITFFS
                     WVGPQLEPDRVQHFLRVTLPERVPWFGAAWDALDPYLAPAVSNLLGQVSAWVQFVIQR
                     LATGVGSFAIELFIFFMFLFFLLRDGRAFLSLLRSLSPLSPEQEARVIEHLAATTKGA
                     LLGILVVPIAQGALATVGYWLFGVPNALLWGCITVIACLIPLLGAPVVWIPITIFLFF
                     TGPAWRGIALFVYGTFVISGIDNVLKPMLLSGTARVHPLPGFLCVVGGTLAFGPAGLL
                     VGPVVLSLAISALHIYRTEFQPGRIHPPTARIETAPARVEPVPGQPGRIEIPPGTTGP
                     "
     gene            1375..3054
                     /locus_tag="VFR25_04330"
     CDS             1375..3054
                     /locus_tag="VFR25_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9940305.1"
                     /translation="MCLAAPAGAQSFLKNIPQTRKPEFRAPGWAPDEVLVQFRNAKDK
                     DEMARAAREEGGELREVVTPDGLARVRITPGTDVPQSMKQWMRRNDVLYAAPNFQARG
                     FFVPNDTTIATFDLAWNLRAIDAYTAWDVARGDPSVVLAIVDTGVAFEDHPIPPNELP
                     FVWPGISTYRQSPELPGPFLQGWDFVHDDAHPNDDNGHGTMVATLAAGLANNVAGSAG
                     IAFGITILPVKVIDYRADGNADDIVQGIRFAADHGADIINLSLGFPPLTFFEFLGLSK
                     QEVDDMFRPLREAIQYAQRRGAIIVAASGNFEADEVSLPAGYQGVIAVGATGFDDLPT
                     SYTSSGKRLDLMAPGGDFTELNNDHVQDAVFNLSIKPFRSDGSLAKPDSFGVFPFFGT
                     SGASPQVAGTVALLESMGLKNQGLIEQILQSTAKQPFVPNHGGWDPRDGYGLLQVGAA
                     VRKAAGNGPASAAGAPAGTLEARLLSGNPARGGAALSFKIAVEGRVRARVFNAKGELV
                     RTMLDAVREAGSHTIAWDGRDERGHAAPSGIYFLRIDANDAVATRKVAFLR"
     gene            3135..3605
                     /locus_tag="VFR25_04335"
     CDS             3135..3605
                     /locus_tag="VFR25_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940306.1"
                     /translation="MNRTAATALLLLTLALTPAAARAGDVPSTTTTTVVKPVDTKETQ
                     LQRIQKMVAKIDAEASTPEGEANVIARMSKQYAVAPDTLEAQHANWGLGYGEIAMVYG
                     FARSSKKKATPDQVVEMRQSGMSWENIGKELGVKVDNVTTRMKKNVNPKPTPHK"
     gene            3722..4804
                     /locus_tag="VFR25_04340"
     CDS             3722..4804
                     /locus_tag="VFR25_04340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET9940307.1"
                     /translation="MTDLEAIARQKLPKGVYDYYAGGAEDEQTVHGNRDAYRHVYLRP
                     RALVGVARVDTATTVLGTPVSLPVLLAPVAYQRMADPEGEVATARAAAAAGTLMVVST
                     IATASIEEVASAAPAAPRWFQLYMAPEREISEHLIARAEAAGYRALCLTVDTPLLGRR
                     ERDVRNRFNPGMALANFEGDIAWMPRTQTGSAFAVGASRLLDPSLTWETVRWMRGRTK
                     LPIIVKGVINAADVEPAIEAGVSGIVVSNHGGRQLDGCEPTLAALPNVVEAAAGRVEV
                     YVDGGVRRGTDVVKALALGARAVLIGRPIVWGLAAAGQAGVATALEMLRSELAMALAL
                     CGCPTIDAIGRELVVTRFAPEPLQRA"
     gene            4836..5276
                     /locus_tag="VFR25_04345"
     CDS             4836..5276
                     /locus_tag="VFR25_04345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9940308.1"
                     /translation="MRSEISIHINAPPDVVYSLVSDLDRWEQWLSHYRYVRILDRDGD
                     GVRAAMSAWRGPIPVFWHAVQKAESATRTIRFRHVRGITTGMDVLWTIEPEENGTRAK
                     ILHDLDLKWPVIGPWFADHVISRGFIEPIAGRTLRSFKNIAELI"
     gene            5273..6544
                     /locus_tag="VFR25_04350"
     CDS             5273..6544
                     /locus_tag="VFR25_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HET9940309.1"
                     /translation="MSEGPHPRRAVVTGLGLVTPIGIGVSSFWEGVTTGRQGVRPLTR
                     FDASELRTRIAGQVDDFDPLDHVDGRRKNRLDRFAHFSIAATRMALEDAGLTIGTNGT
                     PNGIASDRAGSAIGSALGGLPGSEMDHAEFLERGPRSIPASLALRVYAGAGACNVAIE
                     FGVRGPALGNSNSCASGAIAIGTALSMIRSGELDAVIAGGVEAPLAPLTFASFTVIRA
                     MSTANEVPGEASRPFDARRDGFVMAEAGAILILEEMGHAVRRGARIYAELRGFASTND
                     AHHMTAPHPEGLGARMAMRKALGDARLSTEEVELINAHGSSTLLNDRIETFAIKEVFG
                     DRARRIPVSGTKGMHAHALGASGAVEAAIAALSIHEGWVPSTTNLRQPDPECDLDYVP
                     GAGRRATVRTVLSNSFGFGGTNACLVFSGVS"
     gene            complement(6561..7589)
                     /locus_tag="VFR25_04355"
     CDS             complement(6561..7589)
                     /locus_tag="VFR25_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940310.1"
                     /translation="MNRPRIVTLLLALLAVLGASSVRAQAIQYKPLLQNPKVTDTDPA
                     PSPDGKWIAFQSNRSGSSQIWVMPAGGGTPRKVTSEPDSVSSQGRRMPCRVMTPTWSP
                     DSKTILFVSTRSGPYNIYAIPFAGGAKPKPLSRAAGSQRFPVYSPDGQRICFPSSRGD
                     PNSLYGFNLYMMTAAGEINGPPARQLTHSKGSPGHPVWSPDGKWVAYVAKDFDSTRTV
                     NVGNGMQTKQSALFSQFRVYKIPAGGGSETKLTGISAAEQGTAEDTWPSWSPDGKWIA
                     FGRNANGVQNIWVIDVVTRKAFPLTNMGGCMKPTWSADGKSLYFTKLNERDEDLWVAT
                     NLGLATRR"
     gene            complement(7636..8223)
                     /locus_tag="VFR25_04360"
     CDS             complement(7636..8223)
                     /locus_tag="VFR25_04360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9940311.1"
                     /translation="MSAGRDIVFVRHGETDWNRERRVQGSQGASINDAGREQARALSR
                     LLWEVPLQAVYSSALPRALETASYVAGPHSLGVVTDPRLNEIDHGDWEGMLESELPDL
                     PLYLKWRDDPTAVTLPGGESLQAVHERAVQVMREILAKHEEKDGLVAVVSHQVVLALL
                     KCYLLDRPWSQLRRHALSVASYEILTAGEGFTPRP"
     gene            complement(8235..9269)
                     /gene="asd"
                     /locus_tag="VFR25_04365"
     CDS             complement(8235..9269)
                     /gene="asd"
                     /locus_tag="VFR25_04365"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET9940312.1"
                     /translation="MPVAVLGGTGTVGQRFVQLLEGHPWFEIVEVLASDQSAGKPYAE
                     AVGSRWKLTTRIPPGVAKLRVRAPGDKLQGRVLFSALDASVAGELEHEYASRGHFVSS
                     NARNHRMDPMVPLAIPEVNRDHMKLLDLQAYKNGGGLVTNPNCSTIVIAMALAPLHRA
                     FGVDAAIVTTFQATSGAGYPGVPSLDILGNVVPFISGEEGKIESETQKILGRIGPKGI
                     AHAEFPVSALCHRVAVLDGHQMAISLRLKTRTTRKRILDAWEGFRPLQKLGLPSAPDQ
                     PIEARPEEDRPQPRLDAERAGGMGVTVGRLRPCPVLEWKFEAVGHNTIRGAAGAAILN
                     AEILVRDERI"
     gene            9409..>10383
                     /locus_tag="VFR25_04370"
     CDS             9409..>10383
                     /locus_tag="VFR25_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940313.1"
                     /translation="MIPRIASALRSPASRPLAAAVVLALAGALLACGPAFAQALLEPI
                     PPGAEFSSVTLVPGVPTPVGVNVQELNGIAATLTSARADFYAAPLPPGHYGFSVAPLR
                     SMTIPFHASIPRRGTARLNVPVTLPPDLAKQTPDGILGFGLVFEGTDELGLPVSFQAI
                     SGSVGSPVDSLTAVAPLQVVPAMASNLAIQSIALERPLPGAAYAAGDTVRVRAVVTGF
                     GTGSFRVLVTMDGDPVAMENGYMESGRPVTIEPRGPMPSRRLGEHLLHVIVEWPQNVA
                     ARPVTVVCMPPPSGIAPPPKPEEADTLQTEVVPPRPPSAFTLDATYLAI"
BASE COUNT         1542 a         3621 c         3526 g         1694 t
ORIGIN      
        1 cgagccattc ctgcaccatc tcgatcgtca tggcagcact ccccgggaca tgtgctccgg
       61 acggaagccg cagtgtagcg tagagggatg agcgacgagc gggagtactg gccgggcccg
      121 gccgggatga tgaggaccac gatcctcgtc atcacccttc tcgcggtcgt ctggctctcg
      181 ttcctcatct tcagcccgtt cctgctcgcg ttcgcgaccg cggcgccgat cgcgctcctc
      241 ctcttcccca cgcaggagcg tctggccgcg ggcatggggc agcgccgatc cttggccgcc
      301 attctcctcg tgatctggat cgcgctcctc gtcgcgattc cgctcgccgc ggtgctgagc
      361 ctggtcggga gccaggcgat caccttcttc tcctgggtgg gcccgcagct cgagccggac
      421 cgggtgcagc acttccttcg cgtcaccctg ccggagcgcg tgccctggtt cggcgccgcg
      481 tgggacgcgc tcgatcccta cctcgcgccc gccgtatcga atctgctcgg gcaggtctcg
      541 gcgtgggtcc agttcgtcat ccagcggctc gcgaccggag tcggctcctt cgcgatcgag
      601 ctcttcatct tcttcatgtt cctcttcttc ctgctccggg acggccgcgc gttcctctcg
      661 ctcctgcgat ccctgtcgcc gctctcgccc gagcaggagg cgcgggtgat cgagcacttg
      721 gccgcgacta ccaagggagc ccttctcggg atcctcgtcg tcccgatcgc acagggagcg
      781 ctggccacgg tcggatactg gctcttcggc gtgccgaatg cgctcctctg gggctgcatc
      841 acggtcatcg cgtgcctgat cccgctcctc ggcgcgccgg tcgtctggat cccgatcacg
      901 atcttcctct tcttcacggg gccggcgtgg agggggatcg cgctcttcgt gtacggcacg
      961 ttcgtcatca gcggcatcga caacgtcctg aagccgatgc tcctgagcgg caccgcgcgc
     1021 gtccatccgc tgccggggtt cctgtgcgtc gtcgggggca ccctggcctt cgggccggcc
     1081 gggctcctgg tgggaccggt cgtcctgtcg ctcgcgatct cggcgctcca catctaccgc
     1141 acggagttcc agccggggcg aatccacccc cccaccgcac ggatcgagac cgcgccggcg
     1201 cgggtcgagc ccgtccccgg ccagcctggc cggatcgaga ttcctccggg caccacgggg
     1261 ccctgacggg gcagatcgtg atacaatagc ggccttatgg cccgctccag ggcggccggc
     1321 cgttcgcgac gccaactcac acttcctgcg ctactcacgt cgctccttct agcagtgtgc
     1381 ctggccgcac cggctggcgc gcagtccttc ctcaagaaca tcccgcagac ccgtaagccc
     1441 gagttccgcg cccccggctg ggccccggac gaagtgctgg tgcagttccg gaatgcgaag
     1501 gacaaggacg agatggctcg cgccgcgcgg gaagagggcg gcgagctgcg cgaggtggtc
     1561 acgccggacg gtctcgcgcg cgtgcgcatc acgccgggaa ccgacgttcc gcagtcgatg
     1621 aagcagtgga tgcgccgcaa cgacgtcctc tacgcggccc cgaatttcca ggcgcgcggc
     1681 ttcttcgttc ccaacgacac cacgatcgcg acgttcgacc tcgcgtggaa cctccgcgcg
     1741 atcgacgcct acaccgcgtg ggacgtggcg cgcggcgatc cgagcgtcgt gctcgcgatc
     1801 gtcgacacgg gcgtcgcgtt cgaggatcac ccgatcccgc cgaacgagct cccgttcgtg
     1861 tggcccggaa tctcgacgta tcgtcagtcg ccggagctgc cgggaccgtt cctccagggc
     1921 tgggacttcg tgcacgacga tgcgcatccc aacgacgaca acggccacgg cacgatggtc
     1981 gcgacgctcg cggcaggcct cgccaacaac gtcgcgggaa gcgccggcat cgcgttcggc
     2041 atcacgatcc tgccggtcaa ggtgatcgac taccgcgccg acggcaatgc ggacgacatc
     2101 gtgcagggca tccggttcgc ggccgaccat ggcgcggaca tcatcaacct gagcctcggc
     2161 ttcccgcccc tcaccttctt cgagttcctg ggactctcga agcaggaagt ggacgacatg
     2221 ttccgccccc tgcgggaagc gatccagtat gcgcagcggc gtggcgcgat catcgtcgcg
     2281 gcttccggca atttcgaggc cgacgaggtc agcctgcccg ccggctacca gggcgtgatc
     2341 gcggtgggcg ccaccggctt cgatgacctg ccgacgtcct acacgtcgtc cggcaagagg
     2401 ctcgacctca tggcgccggg gggcgatttc acggagctga acaacgacca cgtgcaggat
     2461 gcggtcttca acctgagcat caagccgttc cggtccgacg gctcgctcgc caagcccgac
     2521 tcgttcggcg tgttcccctt cttcggcacc tcgggcgcct cgccccaggt agccggcacg
     2581 gtggccctct tggagtcgat gggtttgaag aaccaaggac tcatcgagca gatcttgcag
     2641 tcgaccgcga agcagccctt cgttcccaac catggcggct gggatccgcg cgacggatac
     2701 ggcctccttc aggtgggtgc ggcggtccgg aaggcggcgg gcaatggccc ggcgtccgcg
     2761 gccggcgctc cggccggcac gctcgaagcg cgcctcctct ccggcaatcc cgcgcgcgga
     2821 ggagccgcgc tttccttcaa gatcgccgtg gaggggcgcg ttcgcgcccg cgtcttcaac
     2881 gcgaagggcg agctcgtccg caccatgctc gatgccgttc gcgaggcggg ctcgcacacg
     2941 atcgcatggg atggccggga cgagcgcggg catgccgctc cgagcggcat ctacttcctg
     3001 cgcatcgacg cgaacgatgc cgtcgccacc cgcaaggtgg ccttcttgcg ctgatggggg
     3061 cgcggggcta gagtcgctcc gttccgatcg ggccgcagtc gcgccgcccg cacgaccccg
     3121 ccaaggagga ccgcatgaat cgcacggccg cgaccgcact actactcctc acgctcgccc
     3181 tgacaccggc cgccgcacgc gccggcgacg tcccctcgac gaccacgacg accgtcgtca
     3241 aacccgtcga caccaaggag acccagctcc agcgcattca gaagatggtg gcgaagatcg
     3301 acgccgaggc atcgacgcct gagggtgagg ccaacgtgat cgcgcgcatg tcgaagcagt
     3361 acgccgtcgc ccccgacacg ctcgaggcgc agcacgcgaa ctgggggctc ggatacggcg
     3421 agatcgcgat ggtgtacggc ttcgcgcgct cgagcaagaa aaaggcgacg cccgatcagg
     3481 tggtcgagat gcggcagtcc ggcatgagct gggagaacat cggcaaggag ctgggcgtca
     3541 aggtggacaa cgtcacgacg cgcatgaaga agaacgtcaa tcccaagccg acgccgcaca
     3601 agtaggcatg gcttcccgcc gaaaacccgc ggtcaggagc gcgaagacgc gccccgcccc
     3661 caagcgcccg ctcgccaagc gcctgcccga ggcgtccccg gagctagaag cctgcgcgtg
     3721 cgtgaccgat ctcgaagcga tcgcgcggca aaagctcccg aagggcgtgt acgactacta
     3781 cgcgggcggc gcggaagacg agcagacggt ccacggaaac cgagatgcct atcgccacgt
     3841 gtacctgagg ccgcgcgcgc tcgtcggcgt ggcccgcgtg gacacggcga cgacggtgct
     3901 cgggaccccg gtctcgctcc cggtgctcct ggcgccggtc gcgtatcagc gaatggccga
     3961 ccccgaaggc gaggtcgcga ccgcgcgcgc cgcggcggcc gccggaacgc tcatggtcgt
     4021 gagcacgatc gcgaccgcca gcatcgagga agtcgcctcg gccgcccccg ccgcgcctcg
     4081 ctggttccag ctctacatgg ctccggagcg ggagatctcg gagcacttga tcgcccgcgc
     4141 ggaagccgcg ggatatcgag cgctctgtct caccgtcgac acgcccctgc tcggacggcg
     4201 cgagcgcgac gtccggaatc gattcaatcc cggcatggcg ctcgccaact tcgaaggcga
     4261 catcgcgtgg atgccccgca cgcagacggg ctctgcgttt gccgtcggcg cgagccgctt
     4321 gctggatccc tcgctcacgt gggagacggt gcgctggatg cgcggccgga cgaagctccc
     4381 gatcatcgtg aagggcgtga tcaatgcggc cgacgtcgaa cccgccatcg aggccggcgt
     4441 ctcggggatc gtggtgtcga accatggagg ccggcagctc gacggctgcg agccgacgct
     4501 ggccgcgctc cccaacgtgg tcgaggcggc ggcgggacgc gtcgaggtgt acgtggacgg
     4561 aggtgtgcgg cgcggaaccg acgtcgtgaa ggcgctcgcg ctcggagcgc gcgcggtgct
     4621 catcgggcgc cccatcgtct ggggtctcgc ggccgctggc caggccggtg tggcgacggc
     4681 gctcgagatg ctgcggagcg agctcgcgat ggccttggcg ctctgtgggt gccccacgat
     4741 cgacgcgatc gggcgcgagc tggtcgtaac acgcttcgcg ccggagcccc ttcaacgcgc
     4801 ttgaccccct ccgggaccgg gcgcacgctt gaaccatgcg ctccgagatc tccatccata
     4861 tcaatgcccc gcccgacgtc gtctactcgc tcgtgtccga cctcgaccgc tgggagcaat
     4921 ggctctcgca ctatcgctac gtccggattc tggaccgcga cggcgatggc gtgcgcgcgg
     4981 ccatgagcgc ctggcgcggg ccgatccccg tcttctggca cgcggtgcag aaggcggaat
     5041 cggcgacccg cacgatccgc ttccgccatg tgcgcgggat cacgacgggc atggacgtgc
     5101 tctggaccat cgagcccgag gagaacggga cgcgggcgaa gatcctgcac gatctcgatc
     5161 tcaagtggcc ggtgatcgga ccgtggttcg ccgaccacgt gatctcgcga ggcttcatcg
     5221 agccgatcgc ggggcgcacg ctcaggagct tcaagaacat cgcggagctg atttgagcga
     5281 aggcccccat cctagacgtg cggtggtcac gggcctcggc ctggtcacgc ccatcgggat
     5341 cggcgtgtcc tcgttctggg aaggggtgac caccgggcgc cagggcgtcc gccccctcac
     5401 gcgcttcgac gcctccgagc tccgcactcg catcgccggc caggtggacg acttcgatcc
     5461 gctcgatcac gtggacggcc gtcggaagaa ccgcctcgac cgcttcgccc acttctccat
     5521 cgccgcgacg cgcatggcgc tggaggatgc cggcctcacg atcggcacga acggcacgcc
     5581 gaacggcatc gcgtcggacc gggcgggatc ggccattggc tccgcgctcg gcggcttgcc
     5641 cgggtccgag atggaccatg ccgagttcct cgagcggggg ccacgctcca ttcccgcgag
     5701 cctcgcgctc cgcgtctacg cgggcgccgg cgcgtgcaac gtggcgatcg agttcggcgt
     5761 gcgcggccct gcgctcggca attcgaactc ctgcgcgtcc ggcgcgatcg cgatcggcac
     5821 ggcgctctct atgattcgaa gcggcgagct ggatgccgtg atcgcggggg gcgtcgaggc
     5881 gccgctcgca ccgctcacgt tcgcctcgtt cacggtgatc cgagccatgt cgaccgccaa
     5941 cgaggtgccg ggcgaggcct cgcgaccgtt cgacgcgcgg cgcgacggtt tcgtgatggc
     6001 cgaggccggg gccatcctga tcctcgagga gatggggcac gccgtgcggc gcggcgcgcg
     6061 gatctacgcg gagctccggg gattcgcgag cacgaacgac gcgcaccaca tgaccgctcc
     6121 gcatccggag gggctcggcg cgcgcatggc catgcggaag gcgctcggcg acgctcgcct
     6181 ctcgaccgag gaggtcgagc tcatcaacgc ccacggatcg tcgacgctac tcaacgaccg
     6241 gatcgagacc ttcgcgatca aggaggtgtt cggcgatcgt gcgcggcgaa tccccgtgag
     6301 cgggacgaag gggatgcatg cgcacgcgct cggcgcgtcg ggagccgtgg aggcggcgat
     6361 cgcggcgctc tcgatccacg agggatgggt tccctcgacg acgaatctgc gccagcccga
     6421 tccggagtgc gatctcgact acgtgccggg ggcggggcgc cgcgcgacgg tgcgaaccgt
     6481 gctctcgaat tcgttcgggt tcggaggcac gaacgcatgc ctcgtcttca gcggcgtgtc
     6541 gtaggcgtcg gcggcgcgcg tcagcgccgt gtcgcgagcc ccagattggt cgcgacccag
     6601 aggtcctcgt cccgctcgtt cagcttggtg aagtagaggg acttgccgtc cgccgaccag
     6661 gtcggcttca tgcatccccc catgttcgtg agcgggaacg cctttcgcgt cacgacatcg
     6721 atcacccaga tgttctggac gccgttcgca ttccggccga acgcgatcca ctttccgtcg
     6781 ggagaccacg aaggccaggt gtcttccgcg gtcccctgct cggccgccga gatcccggtg
     6841 agcttcgtct cggagcctcc acccgcggga atcttgtaga cgcggaactg cgagaagagc
     6901 gcgctctgct tcgtctgcat gccgttaccc acgttcaccg tgcgcgtcga gtcgaagtcc
     6961 ttggcaacgt aggcgaccca cttgccgtcc ggcgaccaga ccgggtgccc ggggcttccc
     7021 ttgctgtgcg tgagctggcg cgcgggcggc ccgttgatct ctccggccgc cgtcatcatg
     7081 tagagattga atccatagag tgaattggga tcgcctcggc tcgaggggaa gcagatccgc
     7141 tggccatccg gcgaatagac cggaaaccgc tggctgcccg cggcgcgcga gagcggtttg
     7201 ggcttcgctc ccccggcgaa cggaatcgcg tagatgttgt agggccccga gcgcgtggag
     7261 acgaagagga tcgtcttgct gtcgggagac cacgtcgggg tcatgacgcg gcacggcatc
     7321 cgccgtccct gcgagctcac cgaatcgggc tccgaggtga ccttccgcgg cgtcccgccc
     7381 ccggcgggca tcacccagat ctggctcgag ccgctccggt tgctctggaa cgcgatccac
     7441 ttcccgtccg gagagggggc gggatcggtg tcggtgacct tcggattctg gagaaggggc
     7501 ttgtactgaa tggcttgcgc ccgcacggac gaagcgccga gcacggcgag aagggcgaga
     7561 aggagcgtga cgatacgcgg acgattcata gggttcctct tcgtgaaccc gcgagcgccc
     7621 aatcggttcc ggcgttcacg ggcgcggagt gaagccttca cccgccgtca ggatttcgta
     7681 ggacgccacg ctcagcgcgt ggcggcggag ctgactccag ggacgatcta aaaggtagca
     7741 cttgaggagc gccagcacga cttggtggga cacgacggcg acgagcccgt ccttctcctc
     7801 gtgcttggcg aggatctcgc gcatgacctg gaccgcgcgc tcgtgcacgg cttgaagcga
     7861 ctcgcccccg gggagcgtca cggcggtggg atcgtcgcgc cacttcagat agagcggcag
     7921 gtcgggcagc tccgactcga gcattccttc ccagtccccg tggtcgatct cgttgaggcg
     7981 aggatcggtg accacgccga gcgagtgcgg accggccacg tacgacgcgg tctcgagggc
     8041 gcgaggcagc gcgctcgaat agacggcctg gagcggcact tcccagagga ggcgcgagag
     8101 cgcgcgggct tgctcgcggc cggcgtcatt gatggaagcg ccctggcttc cctgcacgcg
     8161 ccgctcgcga ttccagtcgg tctcgccatg gcgcacgaag acgatgtccc ggccggccga
     8221 catggagtcg cggactagat ccgctcgtcc cgaacgagga tttccgcgtt caggatggcc
     8281 gcgcccgcgg cgccgcgaat cgtgttgtgg ccgaccgcct cgaacttcca ttcgaggacg
     8341 ggacaagggc ggagccgtcc gaccgtgacg cccatgccgc ccgcgcgctc ggcatcgagc
     8401 cggggctgcg gacggtcctc ctcgggacgc gcttcgatgg gctggtcggg agcgctcggg
     8461 agtccgagct tctggagcgg gcggaagccc tcccacgcgt cgaggatccg cttccgcgtg
     8521 gtgcgcgtct tcaagcgcag gctgatggcc atctggtgac catccaatac cgccacgcga
     8581 tggcagagcg cgctcaccgg gaattccgcg tgcgcgattc ccttcgggcc gatgcgcccc
     8641 aggatcttct gcgtctcgct ctcgatcttg ccctcttccc cggagatgaa cggaacgacg
     8701 ttcccgagga tgtcgagcga ggggacgccg ggatagcctg cgccgctcgt ggcctggaac
     8761 gtggtgacga tcgccgcgtc gacgccgaac gctcgatgga gcggggccag cgccatcgcg
     8821 atcacgatcg tcgagcagtt gggattcgtg acgaggccgc cgccattctt gtaggcctgc
     8881 agatcgagga gcttcatgtg atcccggttc acctccggga tcgcgagcgg caccatcggg
     8941 tccatgcggt ggttgcgcgc gttcgacgac acgaagtgtc cacggctcgc gtactcatgc
     9001 tcgagctcgc ccgccaccga cgcgtcgagg gccgagaaga ggacgcggcc ctggagcttg
     9061 tccccgggcg cgcgcacgcg gagcttggcg acgccgggcg ggatgcgcgt cgtgagcttc
     9121 cagcgagagc cgacggcttc ggcgtagggc ttgcccgcgc tctggtcgga ggccaggacc
     9181 tcgacgatct cgaaccaggg atgcccttcg agcagctgga cgaaccgctg cccgaccgtt
     9241 cccgtaccgc cgagcacggc gaccgggatc ttcctgcgcg ggggcggcgc gacctgtttt
     9301 cgccccgaac tcgttgcggc ggctcgcttt cttgacggca tagacctcgg atgatacggt
     9361 tccccgcgcg tttgccgcaa cgtcattccc actcctcggg gcctccgctt gatccctcga
     9421 attgcatcgg cgctccgttc gcctgcctcg cgcccactcg ccgccgcggt cgtcctcgcg
     9481 ctcgcggggg cgcttctggc atgcggtccc gccttcgctc aggcgctcct cgagccgatc
     9541 ccgcccgggg ccgagttctc gtcggtcacg ctcgtgccgg gggttccgac gccggtcggc
     9601 gtgaacgtgc aggagctgaa cggcatcgcg gccaccctca ccagtgcccg cgcggatttc
     9661 tacgcggctc cgctcccccc gggtcactac ggattctcgg tcgctccact ccgttccatg
     9721 acgattccgt tccacgcgtc aatcccgcgc cggggcaccg cgcggctcaa cgttcccgtc
     9781 acgctgccgc ctgacctcgc gaagcagacg cccgacggga tccttggatt tgggctcgtc
     9841 ttcgagggca cggacgagct gggcttgccg gtgtcgttcc aggcgatctc gggaagcgtc
     9901 ggttcgccgg tcgactcact gactgcggtc gcgccccttc aggtcgtccc ggcgatggcg
     9961 tcgaatctcg cgatccagtc gattgcgctc gagcgaccgc tcccgggtgc ggcttacgcc
    10021 gccggggaca ccgtgcgcgt gcgcgccgtg gtcacgggat tcgggacggg cagcttccgc
    10081 gtgctcgtga cgatggatgg cgatccggtc gcgatggaaa acggctacat ggaatcgggg
    10141 cggccggtca cgatcgagcc gcgcgggccg atgccgtcgc gtcggctggg cgagcacctg
    10201 ctccacgtga tcgtcgagtg gccgcagaac gtcgcggcgc gcccggtcac ggtcgtctgc
    10261 atgccgccgc cttcgggcat tgcgccgccg ccgaagcccg aggaggccga tacgctccag
    10321 accgaggtcg tgccgccgcg cccgccgtca gcgttcacgc tcgacgccac ctacctcgcg
    10381 atc
//
LOCUS       DASTKC010000105         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_551244, whole genome shotgun sequence.
ACCESSION   DASTKC010000105 DASTKC010000000
VERSION     DASTKC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551244"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /gene="tmk"
                     /locus_tag="VFR25_04375"
     CDS             <1..467
                     /gene="tmk"
                     /locus_tag="VFR25_04375"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9940314.1"
                     /translation="ELLLFMAARAQHVSERIRPALEAGQVVVCDRFTDATLAYQGGGR
                     AVPRDLLERLNEAATGGLRPDRTYLLDVDVATGVARAGGRAGQKRDRIESEDAAFFES
                     VRRRYVDLAMEDPERILLLRGTDSREDLARQMLLDALTLLRSRNIQPSRTST"
     gene            464..2104
                     /locus_tag="VFR25_04380"
     CDS             464..2104
                     /locus_tag="VFR25_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9940315.1"
                     /translation="MNARQGFAVIVLVTALIAGTWAVGRVQETSDNTYANIERFIQVL
                     SKVRDHYVEPVTTDKLMDSAIRGMLRTLDPYSQYLDKDEAERLETTTHGSFGGIGISI
                     GIRDSWVTVISPIEGTPAWRAGIQGGDRIIKIDGASTEGLSLDDAMKKMRGDKGTKVE
                     LSIFREGRDKPMDFSITRDIIQIKSVPYAGVLNNGVGYIRLSNFSERSRDEIDDALAK
                     IEKQNPRGLILDLRYNPGGLLSQAVEVSEEFAPRGKKIVFTRGRDATQNRDFYSSSTE
                     PHQKYPLIVLVNQWTASASEIVSGAVQDLDLGVVVGRSTFGKGLVQTVIPLTRSVRGP
                     KLKLTTAKYYTPSGRCIQKEEQLKDGALADSDDDDDSQTADLPDSLRVKKPLPEYKTE
                     MGRTVYGGGGIVPDIELEEVKYPHLVEDLESKQTFFKYAVKYATKHKDPPANYAITPA
                     MREEFMTMVKADKIEVAADSLAMAQKFIDTGMRRELARRYAGDEEAYRVALEDDQQVR
                     EAASLIDKAATLPKLLALANEMSKSRASGNPDKTPSVH"
     gene            2108..3169
                     /gene="dusB"
                     /locus_tag="VFR25_04385"
     CDS             2108..3169
                     /gene="dusB"
                     /locus_tag="VFR25_04385"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET9940316.1"
                     /translation="MQAGDVRLKGRVLLSPLCGVTDSPFRMLAKRHGAAMVFCEMTSS
                     DGLVRKNPKTFELLEYRPEERPIGAQLCGADPVVMADAARMCADLGFDTLDLNYGCPV
                     RKVIAREAGAAMLTDMDRLERVTTAVVNAVPHLPVTAKIRIGWDQNSTNAPDVAKVLE
                     RSGVRWIAVHARARSEKFGGKAHWEVIGHVKEATSIPVIGNGDVKTPEDALRMVQETG
                     CDGVMVGRGSFGNPWLFGRAQRLLEGLDPGPEPTPRERITTAIAHLHDLARKKGDYAA
                     VLMRKHVAWYVRGLYDNSTLCRKINHAQNPAETEALLLEYLDMLERHPEPVLAVASPG
                     NGHGPDGDEANGNGSGDST"
     gene            3147..>3528
                     /locus_tag="VFR25_04390"
     CDS             3147..>3528
                     /locus_tag="VFR25_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940317.1"
                     /translation="MDPATLRKLLENVRRGTLSPARALDELARLPFETAGPALVDHHR
                     ALRQHLPEVILGQGKTVAQCVTIARAVVKKSRRCLATRVNREQAEALLEEFGEDARWN
                     ETARVVAIETARRAVRRGASSKAKA"
BASE COUNT          654 a         1184 c         1184 g          506 t
ORIGIN      
        1 ccgaactgct actctttatg gcggctaggg cacagcacgt gtccgagcgg atccggcccg
       61 ccctcgaagc gggtcaggtc gttgtgtgcg atcgttttac ggacgctacg ctcgcgtatc
      121 aggggggcgg gcgcgccgtt ccgcgggacc tcttggaacg cctcaacgag gcggccaccg
      181 gggggctccg accggaccgg acctacctcc tcgacgtcga cgtcgcgacc ggggtcgccc
      241 gtgcgggcgg ccgcgccggt cagaagcgcg accggatcga gagcgaggac gccgccttct
      301 tcgagtcggt gcgccggcgc tacgtcgatc tggccatgga agatccggag cggatcttgc
      361 tcttacgggg tactgactcc cgagaggatt tggcgcgtca aatgctatta gacgcgctca
      421 cgctgctccg cagccggaac attcagccat cgaggacttc cacatgaacg caaggcaagg
      481 attcgcggtg atcgtcctgg tcacggcttt gatcgccggg acctgggccg tcgggagggt
      541 ccaggagacc tccgacaaca cgtacgcgaa catcgaacga ttcatccagg tcctcagcaa
      601 ggttcgcgac cattacgtgg agccggtcac cacggacaag ctgatggatt cggccattcg
      661 cggcatgctt cggaccctcg atccctactc gcagtacctg gacaaggacg aggccgagcg
      721 cctcgaaacg accacccacg gctccttcgg cggcatcggg atctcgatcg gcatccgcga
      781 cagctgggtg acggtgatct cgcccatcga gggcacgccc gcatggcggg ccgggatcca
      841 ggggggcgac cggatcatca agatcgacgg agcgtccacg gagggcctgt ccctcgacga
      901 cgccatgaag aagatgcgcg gcgacaaggg aaccaaggtc gagctctcga tcttccgcga
      961 gggccgcgac aagccgatgg acttctccat cacgcgcgac atcatccaga tcaagagcgt
     1021 gccctacgcg ggcgtcctca ataacggcgt cggctacatc cgcctctcca atttctccga
     1081 gcggagccgg gacgagatcg acgacgcgct cgccaagatc gagaagcaga atccacgcgg
     1141 gctgatcctc gacctccggt acaacccggg cggtctcctg tcgcaggcgg tcgaggtgtc
     1201 cgaggaattc gcgccgcgcg gcaagaagat cgtcttcacg cgcggccggg acgcgacgca
     1261 gaaccgcgac ttctactcgt cgtccacgga gccgcaccag aagtatccgc tcatcgtgct
     1321 cgtgaaccag tggaccgcca gcgcgtcgga gatcgtgtcg ggcgccgtgc aggatctcga
     1381 cctcggcgtc gtggtcggac ggagcacgtt cggcaagggg ctcgtccaga ccgtgatccc
     1441 gctcacgcgc agcgtgcgcg gcccgaagct caagctgacg acggcgaagt actacacgcc
     1501 gagcggccgc tgcatccaga aggaggagca gctcaaggac ggcgccctgg ccgacagcga
     1561 cgatgacgac gacagccaga ccgcggacct cccggactcg ctccgggtga agaagccgct
     1621 ccccgagtac aagaccgaga tggggcgcac cgtgtacggc ggcggcggga tcgttccgga
     1681 catcgagctc gaggaagtga agtacccgca cctggtcgag gacctcgagt ccaagcagac
     1741 cttcttcaag tacgcggtca agtacgcgac caagcacaag gatccgcccg cgaactacgc
     1801 gatcacgccg gccatgcgcg aagagttcat gaccatggtc aaggcggaca agatcgaggt
     1861 cgcggcggac tcgctggcga tggcgcagaa gttcatcgac accggcatgc ggcgcgagct
     1921 cgcgcggcgc tacgccgggg acgaggaagc ctacagggtc gcgctcgagg acgatcagca
     1981 ggtccgcgaa gcggcctcgc tcatcgacaa ggcggcgacc ttgccgaagc tcctcgcgct
     2041 cgcgaacgag atgagcaagt cgcgcgcgtc gggcaatccg gacaagacgc cgagcgtcca
     2101 ctagcggatg caggctggtg acgtccgact gaaagggaga gttctgctgt cgccgctctg
     2161 cggcgtcacg gactctccct ttcgcatgct cgcgaagcgc catggcgccg ccatggtctt
     2221 ctgcgagatg acctcctccg acggcctcgt ccggaagaat cccaagacct tcgagctgct
     2281 cgagtaccgc cccgaggagc gccccatcgg cgcacagctc tgcggcgccg atccggtcgt
     2341 catggccgac gccgcgcgga tgtgcgccga cctcggcttc gacacgctcg acctcaacta
     2401 cggctgcccc gtccgcaagg tgatcgcgcg cgaggcgggg gcggccatgc tcaccgacat
     2461 ggatcgcctc gagcgcgtca cgaccgcggt cgtgaacgcg gtgccgcacc ttcccgtcac
     2521 cgcgaagatc cggatcggct gggaccagaa ctccaccaac gcccccgacg tggcgaaagt
     2581 gctcgagcga tcgggcgtcc gctggatcgc ggtgcacgcg cgggcgcgct ccgagaagtt
     2641 cgggggaaaa gcgcactggg aagtgatcgg ccacgtgaag gaagccacgt cgattcccgt
     2701 gatcgggaat ggcgacgtga agacccccga ggacgcgctc cggatggtcc aggagaccgg
     2761 ctgcgacggc gtgatggtgg gacgcggctc gttcggcaat ccctggctct tcggccgcgc
     2821 gcagcgtttg ctcgaagggc tcgacccggg tcccgagccc acgcctcgcg agcgcatcac
     2881 gaccgcgatc gcgcacctac acgatctggc gcggaagaag ggggactacg ccgccgtgct
     2941 catgcgcaag cacgtggcgt ggtacgtgcg cgggctctac gacaactcca ccctttgccg
     3001 gaagatcaac cacgcgcaga atcccgccga gacggaggcg ctcctcctcg agtatctcga
     3061 catgctggag cgtcaccctg agcccgtcct cgccgtagcg tcgcccggga acggccacgg
     3121 accggacggc gacgaggcga acgggaatgg atccggcgac tctacgtaag cttctcgaga
     3181 acgtccgccg cgggacgctg agtcccgcgc gcgccctcga cgagctggcc cggctcccct
     3241 tcgaaaccgc cggccccgcg ctcgtcgacc atcaccgcgc cctcaggcag catctccccg
     3301 aagtcatcct cggccaggga aagaccgtgg cgcagtgcgt gacgatcgcg cgcgccgtgg
     3361 tgaagaagag ccggcgatgc ctcgccacgc gcgtgaatcg cgagcaggcg gaggcgctgc
     3421 tcgaggagtt cggggaggac gcgcgctgga acgagacggc tcgggtggtg gccatcgaga
     3481 cggcgcggcg cgctgtacgg cgcggcgcga gctccaaggc gaaggcac
//
LOCUS       DASTKC010000106        21502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1262639, whole genome shotgun sequence.
ACCESSION   DASTKC010000106 DASTKC010000000
VERSION     DASTKC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262639"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFR25_04395"
     CDS             <1..512
                     /locus_tag="VFR25_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9940318.1"
                     /translation="LPRVVVDAAPRAHATVPSGSPRRILVADDNEDSAESLALLLEAH
                     GHQVQVAHDGESALAVAERFRPDVAFIDIGMPKRSGYEVAEELRLRPWSESVRLIAVT
                     GWGQESDRRRAQEVGFDAHLVKPATPEALFGLLASFDTPRAGRAAEPSIAAQPPSETA
                     ASAGPATPA"
     gene            589..1572
                     /locus_tag="VFR25_04400"
     CDS             589..1572
                     /locus_tag="VFR25_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940319.1"
                     /translation="MKIETTLAGSYPKLPTEPGDVNLRVTKNRRDQGKATDQDVLEAV
                     RETTRRILKIQERAGVDIPVDGSAAWDDAQTYVARGIGGFQIEGLIRYLDTNTYYRQP
                     AIVGRIQWERPVTVEDWKLAQSMSERPVKAFLPGPYSLYHFSRDRHYKSPAEALADLG
                     QALGKEAQALEEAGARWIHFEEPWLGKAKSQDAALVKTALEPLLKGRKARTVIHVPFG
                     APTAVWDSLGELPWSVIGLDLREAPEGWDLIPRVPKGRTVALGLIEARNTRLEDAAEV
                     AREVARARDLRSDLEYQLCTTASLEYLPADKAEAKVARLAEAARIASRNGG"
     gene            1575..2552
                     /locus_tag="VFR25_04405"
     CDS             1575..2552
                     /locus_tag="VFR25_04405"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET9940320.1"
                     /translation="MPRGLWTTTVGSFPKPPRLEKARNQHARGELKADALEAMEREET
                     IEVIRRQERIGLDIVVDGELYRGDMTTYFAERMPGFAISNPVRSYGNRYYRKPIAVGP
                     IRRKKPLTVDWWKFAQAQTSKPVKGMLTGPYTMMDWAFNEHYPSREAMAMDLAEAINE
                     EALALQEAGAKYIQIDEPAVSVRPEELPLAVKALARAAQGLKAKTITHICYGDFDVIY
                     PALLDLPVDQIDLEMANSRYDLLDRFRKHPFTKEIGFGVLDVHSHRIETKEEIKDGIR
                     KALEVLRPEQIYVDPDCGLKTRTVDEAFAKLEVMVEAVREIRAEKGIDV"
     gene            2549..3790
                     /locus_tag="VFR25_04410"
     CDS             2549..3790
                     /locus_tag="VFR25_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9940321.1"
                     /translation="MTRTGSADLSGLTIDRDAKPPTSNRKPIAIALAALVVVAAGATV
                     ALSMVRGKPADVRVAKAEPLGGAPGTVSSEVLTASGYVVARQRASVSTEVAGRLEALY
                     VSEGSRVAQGQVLGVLRNQDQKAAMETAKAALDQASASLAEARANAHEIALRLARTKE
                     LLTRALVSQADYDAIEAQDGVAKARVTSAMAAVESARSRMNAARIEYDKTFIRAPFAG
                     AVLRKEAEVGEIVSPIPSSGGLTRGAIVTMANLGTLEVEVDVNEGYVARVREGMRAEI
                     ALDAYPTDRYPGHARQIVPTADRQKATVQVKVAFDSLDARVLPEMGAKVSFLADPEAA
                     PAGGASTSSAVWVPASAVREQAGRAVVYVVDDGRATARGVAPRPLGPDRVMIASGLAA
                     GEMVVIEGPNDLADKTRVRVR"
     gene            3829..4524
                     /locus_tag="VFR25_04415"
     CDS             3829..4524
                     /locus_tag="VFR25_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940322.1"
                     /translation="MTEGQGDSGPLISLRDVHKEYRRDSFVVPVLAGITLDVKRGEFL
                     ALMGPSGSGKSTLLNLIGGIDRPTKGEVRVAGEALNALNPSKLAAWRARHVGFIFQLY
                     NLIPVLTAYRNVELPLLLTRLSASERKKRVELALSLVGLEGRDGHYPRQLSGGEEQRV
                     AIARAVVADPTLLVADEPTGDLDSKNAEEILVLLRRLNKELGKTILMVTHDPHAAETA
                     DKTLHLDKGVLRS"
     gene            4521..5669
                     /locus_tag="VFR25_04420"
     CDS             4521..5669
                     /locus_tag="VFR25_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9940323.1"
                     /translation="MKYLRYVLSHLWQSKRRTFLTVSSIAVALFLFCTLRTVMTSLDA
                     GLRASDDTRLVVRHAASLVFPLPLAYRDRLAQVPGVVKVGFGNWFGGYYQDEKNQFTQ
                     FAVDVPTVFDLYPEILLGPAEKEAFQRERTACIAGEALVKKYGWKLGDKIPITGTIYP
                     GEWPLILRGIYRAATPDADENAIFFHWDFLNESMPEGRKNLVGIYWLKVAAPSDAPRV
                     SAAVDQIYENSPVPTKTETEKAFQAGFIQMMGNVSTLLTILGSAIIFAILLVTVNTMM
                     MAARERTTEIAVLKTLGFRDGLVLQLVAAEAMLLSLAGGIIGCGLAFLLFRKVNFTAG
                     GMIPNFRVLPETLLLGILLAIAMGLLSGLVPAFSAARLPIASSLRKVA"
     gene            5669..6835
                     /locus_tag="VFR25_04425"
     CDS             5669..6835
                     /locus_tag="VFR25_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9940324.1"
                     /translation="MARIPLAYNLRNLAARRVSTGLTALGIALVAWVFIFTLALAGGF
                     QAALTTTGTKENAIIIRKGSTSELMSILTREAAGAIESQGEIARAPDGTPLAASELMV
                     VWNLPRKSGTTTNVVVRGVGPKSFALRPQVKLIRGRMFQQGLDEIMVGRMVADRFQHC
                     DVGDQLKLAGREWTVVGVFDSNGRTYDSEIWGDVELFMPVFDRPVYQSLTLRLAEPGY
                     LAPLKKRLESDPRMTVEVEREDKFYAKQSGQLSSMLRVLGLFVTSIMSLGAIFGALNT
                     MYAAVGNRTREIGTLRAIGFSRGAILTSFLFESVLLALLGGALGCLLALPVRSFTTGT
                     MSFATFSELAFRFQVTPGMLLAGLAFSAFMGLLGGFLPARKAATMPIVDSLRQA"
     gene            6909..7817
                     /gene="hemC"
                     /locus_tag="VFR25_04430"
     CDS             6909..7817
                     /gene="hemC"
                     /locus_tag="VFR25_04430"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9940325.1"
                     /translation="MSHAVSSLKLGVRPSRLGRCLGERIAEQAREHLSGPVAFTPVAE
                     DVDPTGAVFGWPGSSLERALLSGQVDAAVLSVKDISPDLDPALTIAAVTERFTPFDVF
                     IARDETILDELPDGAELSAHTSIRRAQLLRYREDFQLLDVPGSLDDRIRILDAGGLDG
                     IVVSASAVEHLGYQDRVNEIFTTELILPAPGQGACAVLTRSDDKERLKLFRPLDHPMA
                     RAEVECERAFLLELGAEPGSAVGALAAFDGTSIRLEGAVADETGKRLVRDAEEGESGE
                     EAEIGRRLARRLIVEGARRIIAAAAR"
     gene            7821..9098
                     /gene="serS"
                     /locus_tag="VFR25_04435"
     CDS             7821..9098
                     /gene="serS"
                     /locus_tag="VFR25_04435"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9940326.1"
                     /translation="MLDLKRVRTETDRVREAAGWKKCQVDLDRLLHLDEERRALIMKS
                     EALKAERNKASEEIGKKKKAGQDAADAVAAMKRVGDEIKDLDGTLAELDQAIESIQIW
                     IPNVPHESAPRAADATGNQVVASWGEIPAFAFEPKPHWELAESLGLLDFTRAAKIAGS
                     GFLLFTGKGARLERALIQFMLDLHTRKHGYVELSPPHLIRRDCLFGTGQIPKMEDDMY
                     RLLGEDLFLNPTAEVPVTNVYRDEILEGERLPIYHTAYCASYRKESGAAGRDTRGMLR
                     VHQFDKVELVKFVRPETSYDELESLRADAEAVLRALELPYRVLLLAAGDLSFAAAKCY
                     DLEAWAPGEKRWLEVSSCSNFEDFQARRAGIRFRPERGAKPEYVHTLNASGVALPRTY
                     AALLETHQTERGTVRVPEALVPYLDGVREITPA"
     gene            9107..10405
                     /locus_tag="VFR25_04440"
     CDS             9107..10405
                     /locus_tag="VFR25_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9940327.1"
                     /translation="MPALPTRRLSYRIWFFLAVLVLMLVLVAHSNAMIRRLNEETRAR
                     CDVLARFFAVATFQAVEDPAVRPIFRDVVRSIDFPVVLTDYAGIPRAWKEIGIAPDAI
                     PDSVIERAAATGVIPPEVERIQHIVERLDRIHQPVDIVEVGKNGALGKVHYGEPNLVK
                     ELRWLPYLELFVILVLLLFGYTGLRSLMTAEQRSLWTALAKETAHQLGTPLSSLMGWT
                     SLLRESPPERLTPERTRDILDEMDRDLARLQKVTLRFGQVGSLPVLKEGDLVEITAGT
                     VDYFRTRLPHLGHAIRIEERYEPVPRVRVHRELFEWVVENLLRNALDAIDRPDGWIEV
                     TVRWLREERTVEMIVRDNGRGMTLDERRRAFTPGFTTKRRGWGLGLALARRVVAEYHG
                     GRISIVESVPGRGTAVAVALPVPPPPDSRATKPSEGQSAS"
     gene            10402..11964
                     /locus_tag="VFR25_04445"
     CDS             10402..11964
                     /locus_tag="VFR25_04445"
                     /inference="COORDINATES: protein motif:HMM:NF020249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9940328.1"
                     /translation="MNVTPRKVLWADDEIDLLKPHILLLEEKGFLVTPVSNGHDAVAC
                     ASREKFDAVLLDESMPGMGGLKTLEAIKEKDPTVPVVLVTKNEAESLMDDAIGRRIDD
                     YLLKPVLPQQIFLALKRLLESDRLQESRRARDYVAELARLRVESPSTLDWRGWIDLYA
                     RSAQWDVELDQVDDPGLKQAQGDQRRELNLEFSSHVERSYESWLSGTDRPALSTDIVE
                     RWVAPHLREGKRVALIVIDCMRLDQWLRIEPLLRPLYDITVDHQLSILPSATPYSRNA
                     IFSGLFPAEIQKEHPEFWQETTSDERSKNRFERQLLEHQLARIGVKLPSGLKYLKVYN
                     TDEAANVRRNVVTYQNIPLVAMVFNFLDMLTHGRSESDLLQELAPDESAFRSVMNSWF
                     RHSALFDALKVMARQDQVVVVTSDHGAILSRRSALVYGNRETSTNLRFKYGTNLGSDP
                     KQAVQIKDPARFKLPADQPHKQYILAKEDFYFIYPTKFHEYESKYRGSFQHGGISLEE
                     MILPVATLVPKG"
     gene            11966..12478
                     /gene="tsaE"
                     /locus_tag="VFR25_04450"
     CDS             11966..12478
                     /gene="tsaE"
                     /locus_tag="VFR25_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET9940329.1"
                     /translation="MDGMEIELRSLLRTEGETRSFGRHLGTLLRPGDWVALTGDLGSG
                     KTTLVSGILESLAPGARGRSPTYVLVEVHPGPVPVVHADLYRLRSLSELPGLALDDLA
                     EPGAVVLIEWADRAEGNLPAVRLDLALRYLDGPGRELRVRPHGARWNQAARDGLFDRD
                     RWQHVLHPGN"
     gene            12456..13127
                     /gene="tsaB"
                     /locus_tag="VFR25_04455"
     CDS             12456..13127
                     /gene="tsaB"
                     /locus_tag="VFR25_04455"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9940330.1"
                     /translation="MSYILGIDTSAPEGSVALARDGRGGAIEMLPPGGHSSGLSAAVG
                     RLLEAAGIGLDGVAAFAVNEGPGSFTGLRIGLAWAKGAALGSNRPLVLVRAHEAMALA
                     HAADAERLVTAIPGARGLVEAALWEKGAASWGPDTVEEGDLIPTLLERGTQGLGIVPA
                     TAKLARSLAEEAAEEGIAILEWRPLAPAVAAIGARLLDQGQAADLTLASPAYGREPNA
                     RKPGS"
     gene            13124..13600
                     /gene="rimI"
                     /locus_tag="VFR25_04460"
     CDS             13124..13600
                     /gene="rimI"
                     /locus_tag="VFR25_04460"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET9940331.1"
                     /translation="MNASPAPLSKLRIGSLSVARIDEVLEIEKLSFTDPWSREMFRSE
                     LEVGGGTYARMAERDGRLLGYSLAVLVADEAHLGNLAVHPDARGAGVGQALLDDLLQA
                     AEKRRATRVTLEVRESNDRARKFYYRNGFIDVAIRKNYYRNPVEDAIVMLRSLRGG"
     gene            13603..14427
                     /gene="accD"
                     /locus_tag="VFR25_04465"
                     /pseudo
     CDS             13603..14427
                     /gene="accD"
                     /locus_tag="VFR25_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
     gene            14670..15980
                     /locus_tag="VFR25_04470"
     CDS             14670..15980
                     /locus_tag="VFR25_04470"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET9940332.1"
                     /translation="MKVTEGASLTKRLDELYRFERSAMRPGLAGVERLLARAGRPDLA
                     FPSILIAGTNGKGSTAAHLDSILRAAGLKTGLYTSPHLVRFGERIRVGGREISEPELE
                     ELLATWWPRFEAENPSFFEAATALCFDHFARSGIDMAVVEVGLGGRLDATNILEPRVA
                     IITTIANDHADILGPTLRRIAGEKAGILKKGGRLVLGVRAGEPRDVILAAARELDIPV
                     TLLGKDGSYGSTRLTPEGTEFHLRTRTLRARVGTPLLGRHQARNAALAALAAEIALQG
                     MPDPSIARAIDSGLRNTRWPARAEILPGNPPILIDVAHNLEGAMALVETVHALWPARP
                     LAVVAGFSRDKEHEAILATLARVGSRFYLTPFEGERAAPMERVLEAARAAGVAFDSVP
                     DVTVALALAKAWAKERKGAVLLAGSVYLAGAAMPALALEVPHAL"
     gene            15970..19509
                     /locus_tag="VFR25_04475"
     CDS             15970..19509
                     /locus_tag="VFR25_04475"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HET9940333.1"
                     /translation="MRSRGAPLLAAIAVLFAALAVGAALAQPPPPGTVAPGGKDDEPY
                     RLTADRLEGSASAGENVYTAIRPTVVHGTTTVTGDSALIYKNRELVLFRGNVKIVDGT
                     THMWGREASYDRKTRLATLRGNVRIEEGSAKITGTEARFYRTENRSVITGNPRLEDST
                     RTLTADRIEYDRTNDVAVAIGKVDAIDRAESTRVRAGRVRYDRRADYAWATEDPVLVR
                     EERKGQHTTVRGDTLEFDNPNRRAYAIGNVRVEREKLRATGTRAEFDQAQDRAILVGK
                     PRAWDDQGVAYGDTIQIEFEDNALRTLRIHPHAVVEFTAKADSGRGERNVASGDTITV
                     HFEKEEAREALMVGHAESRYWPSSADSSEGGRNVSTGDSIRVFFDKGEPARATVRGGG
                     RGTYFLAAEGDTARERQQDLVKYEGDEIQYDIKKGTVDIVGSSDVTYREVHLTADKVT
                     FDSNTDRMRAEGKPVLTDSGNRMTGASMTYDLNTKQGTVFEGRTAYDRGFLFGERVRR
                     VSDTELDVAHGTYSTCDLAEPHFHFESAKMKVILHDKVVARPVVFYIKKVPVLALPFY
                     VFPIQTGRHSGFQLPQVEFGSATGGGKFVRNVGYYWAINDFMDATGWFDYYQQDSWIG
                     HGLYRYNHRYEYQGQISASYQNQFSTGSNRWDLFARHYQILGRAFTLTSEANLTNSSA
                     YRRDQNLGQNVFVRTQRNLRSNLSLQKGWSGGSFALGLLQTEDLDAEPGNLKTEQQLP
                     SFTLTLNARPLGHAARGREPAKWPAFMSTVYSTSLRGLYQRREYEPTTHPDTTVVFDS
                     LAVPIDTTITTVSVDSLDARGAARYDFTLTDVRSLGFIKVAPRFNFAAVYYSRDQAGQ
                     ENQVGTVWGAGIGANTAVTGTFRTGLGPLQAIRHVVTPSAALVYQPPRTSLLYRGPDG
                     FLRPRFDGVSGISLAAAEVRRLDFSLRQDLHVKWGDPIHPHVINNLFQLQNNISYNEL
                     AKRSGAKPLSDMSTSLTFKPIDRSDFAFNFVHNPYDWKLLAFSASTGIALQGQSPTGE
                     ENSIADQEPGTYAVRERSPLTPVGMSPAGLPWSVFASISYQGSSARDGRGGYNPWQST
                     ARLNGGTAVNFTKNWRFEYNWQFDPRAGVMVSQFFTVKRDLHCWEMQFTRSLSGDVGD
                     EYYFKINVKNLPEVYFEQGSRGLRGFGALQNIY"
     gene            19605..19895
                     /locus_tag="VFR25_04480"
     CDS             19605..19895
                     /locus_tag="VFR25_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9940334.1"
                     /translation="MNKTELIAVIAKKTSLSAREARAVVDTLFSPGKEGLIASALMSG
                     KKVQITGFGTFETRKRKAREGRNPQTGERIKIAASRFPAFHAGKALKDRVRK"
     gene            20258..21397
                     /locus_tag="VFR25_04485"
     CDS             20258..21397
                     /locus_tag="VFR25_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9940335.1"
                     /translation="MGELKFPNNFTIDCGDFFGVGGEQDSLKSAFLVHAMDKLGYDVV
                     TLGEREFNFGQQFLLSQFKTTKVDVVCANLVYADSKKPFVKPYVIRKVGTLKVAFFGL
                     LGKDMKIRPFKGDPALTILDPTETGKAMLADLRKKADIVVLLSHLGMSDSQKLTVDVP
                     VDVMFFGHQPGLFRTLAKTQDVITVRGGERGQYIPEVHLVVEDKKITSFDGDVVQLDD
                     KVPADEAMNAEVDKYQDALNQRFAKANEATAQTATAHTAQQIAGERYLGENNCRRCHE
                     AEYQKWSTQKHAHAFQTLITNQRDSTPECLPCHVVGMGSAGGFISKQTTPDLVNVQCE
                     SCHGMGTRHGMADTVGPEVCQTCHTHEQDPNFQYDEMVQKIVHWE"
BASE COUNT         3584 a         7365 c         7324 g         3229 t
ORIGIN      
        1 ggctcccgcg cgtcgtggtc gacgcggcgc caagagcgca cgcgacggtt ccgtcgggat
       61 cgccccggcg catcctcgtc gccgacgaca acgaagactc ggccgagagc ctggcgctcc
      121 tcctcgaggc gcacggccat caggtgcagg tggcccacga cggagagtcg gcgctcgcgg
      181 tggcggaacg cttccgcccc gacgtcgcgt tcatcgacat cggcatgccg aagcgaagcg
      241 gctacgaggt cgcggaagag ctgcgcctcc gtccctggag cgagtccgtc cgcctgatcg
      301 cggtgacggg ctggggccag gagtccgatc gaaggcgggc gcaggaggtc ggcttcgacg
      361 cccacctggt gaagccggcc accccggagg ccctcttcgg cctcctcgcg agcttcgaca
      421 cgcctcgcgc cggccgcgcg gccgagcctt ccatcgcggc gcaaccaccg agcgaaaccg
      481 cggcctccgc ggggccagcg accccagcct gaatcctccc cggccccacg cgggtctgta
      541 gtacaatccc cagcttccca acgtgaaggg cacaatccag gaggacgcgt gaagatcgaa
      601 accacgctcg ccgggagcta tcccaagctt ccgaccgagc cgggtgatgt caatctcagg
      661 gtgaccaaga accgccgcga tcaaggcaag gcgacggacc aggacgtgct cgaggcggtg
      721 cgcgagacga cgcgccgcat cctcaagatc caggagcggg ccggggtcga catccccgtg
      781 gacggctccg ccgcctggga cgacgcgcag acctacgtcg cgcgcggcat cggcgggttc
      841 cagatcgagg ggctgatccg ctacctcgac accaacacct attatcggca gccggcgatc
      901 gtgggccgga tccagtggga gcggcccgtc acggtcgagg actggaagct cgcgcagtcc
      961 atgtccgagc gtccggtgaa ggcgttcctc ccgggcccct attcgctcta ccacttctcc
     1021 cgcgatcggc actacaagtc gcccgcggaa gcgctcgccg atctgggcca ggcactcggc
     1081 aaggaggctc aggcgctcga ggaggccggc gcgcgctgga tccatttcga agagccgtgg
     1141 ctcggcaagg cgaagagcca ggacgccgcg ctcgtgaaga ccgcgctcga gcctcttctc
     1201 aaaggacgca aggcacgcac cgtgatccac gtgccgttcg gcgctccgac cgccgtgtgg
     1261 gattcgctcg gcgagcttcc ctggtcggtg atcggtctcg acctgcgcga agcgccggaa
     1321 gggtgggatc tgatcccgcg cgtgcccaag ggccgcacgg tcgccctcgg cttgatcgag
     1381 gcgcggaaca cgcgcctcga ggacgccgcc gaggtcgccc gggaggtcgc gcgcgcgcgc
     1441 gacctccggt ccgatctcga ataccagctc tgcaccacgg ccagcctcga atacctcccc
     1501 gcggacaagg cggaggccaa ggtggcccga ctcgccgagg cggcacgcat cgcctcgcgg
     1561 aacggaggat gacgatgccc cgcggccttt ggacgacgac ggtcggcagc ttccccaagc
     1621 ccccgcggct cgagaaggcg cgcaatcagc acgcgcgcgg cgagctcaag gcggacgcgc
     1681 tcgaagcgat ggagcgcgag gagaccatcg aggtcatccg gcgccaggag cggatcggcc
     1741 tcgacatcgt ggtggacggc gagctctacc gcggcgacat gaccacgtac ttcgccgagc
     1801 gcatgcccgg cttcgccatt tcgaatccgg tccgctcgta cggcaaccgc tactaccgga
     1861 agccgatcgc ggtcggcccg atccgccgga agaagccgct cacggtggat tggtggaagt
     1921 tcgcgcaggc gcagacgtcg aagccggtga agggaatgct caccgggccc tacacgatga
     1981 tggactgggc cttcaacgag cactatccgt cgcgcgaggc gatggcgatg gatctggccg
     2041 aggcgatcaa cgaggaggcg ctcgcgctcc aggaggcggg ggccaagtac atccagatcg
     2101 acgagcccgc ggtctccgtg cgccccgagg agctgccgct cgcggtgaaa gcgctcgcgc
     2161 gcgccgcgca ggggctcaag gcgaagacga tcacgcacat ctgctacggc gatttcgacg
     2221 tcatctaccc ggcgctcctc gacctcccgg tcgatcagat cgatctggag atggcgaaca
     2281 gccgctacga cctgctcgat cggttccgca agcatccctt cacgaaggag atcggcttcg
     2341 gcgtgctcga cgtgcactcg catcggatcg agacgaagga agagatcaag gacggcatcc
     2401 ggaaggccct cgaggtgctt cgccccgagc agatctacgt cgatccggat tgcgggctga
     2461 agacgcgcac ggtcgacgag gccttcgcga agctcgaggt gatggtggag gcggtgcggg
     2521 agatccgggc ggagaagggc atcgacgtgt gacacggacg ggctcggccg atctgagcgg
     2581 cctcaccatc gatcgagacg ccaagccgcc gacatccaac cggaaaccga tcgcgatcgc
     2641 gctcgcggcg ctcgtcgtcg tggccgcggg cgcgacggtc gctctctcca tggttcgcgg
     2701 gaagccggcg gacgtgcgcg tggccaaggc ggagccgctc ggcggcgcgc cggggacggt
     2761 ctcgtcggag gtgctcaccg cgagcggcta cgtcgtcgcc cgccagcgcg cttcggtctc
     2821 gacggaagtc gcgggtcgcc tcgaggctct gtacgtgagc gagggaagcc gcgtcgccca
     2881 gggacaggtg ctcggcgtgc tccggaatca ggatcagaaa gccgcgatgg agaccgcgaa
     2941 agcggccctg gaccaggcgt ccgcttcgct cgcggaggcc agggcgaacg cgcacgagat
     3001 cgcgctccgg ctcgcgcgca cgaaggagct cctcacgcgc gccctcgtga gccaggccga
     3061 ctacgacgcc atcgaagcgc aggacggcgt ggcgaaggcg cgggtgacga gcgccatggc
     3121 cgccgtcgag agcgcgcgct cgcgcatgaa tgccgcgcgc atcgaatacg acaagacctt
     3181 catccgcgcg cccttcgcgg gcgccgtcct ccggaaggag gcggaggtcg gcgagatcgt
     3241 gagcccgatt ccgtcgagcg gcggcttgac gcggggcgcc atcgtcacga tggccaacct
     3301 gggcacgctc gaggtcgagg tggacgtgaa cgagggctac gtggcgcgcg tgcgcgaagg
     3361 gatgcgcgcg gagatcgcgc tcgacgccta tcccaccgac cgatatccgg gccacgcgcg
     3421 gcagatcgta ccgaccgcgg accgccagaa ggcgacggtg caggtgaagg tcgcgttcga
     3481 ttcgctcgac gcgcgggtcc ttccggagat gggggcgaag gtctcgttcc tcgccgatcc
     3541 agaagcggcg ccggccggcg gagcttcgac gtcgtccgcg gtctgggtcc ctgcgtcggc
     3601 cgtgcgcgag caggcggggc gggcggtcgt ctatgtcgtg gacgacggac gcgcgaccgc
     3661 gcgaggcgtg gcgccgcggc cactcggccc cgaccgcgtg atgatcgcga gcgggctcgc
     3721 ggcgggcgag atggtggtga tcgaggggcc gaacgacctc gcggacaaga cccgagtgcg
     3781 ggtgcgatga ccatgacgaa cacgatgact gagccaagga gcgccacgat gacggaagga
     3841 cagggagact cgggccccct catctcgctt cgcgacgtgc acaaggaata ccgccgggac
     3901 tcgttcgtcg tcccggtgct cgccgggatc acgctggacg tgaagcgggg cgagttcctc
     3961 gcgctcatgg ggccgagcgg atcgggcaaa tcgaccctcc tgaacttgat cggaggcatc
     4021 gatcgtccga cgaaggggga ggtgcgcgtc gcgggcgagg ccctgaacgc gctcaatccc
     4081 tccaagctcg cggcctggcg cgcgcgccac gtcggattca tcttccagct ctacaacctc
     4141 attccggtgc tgaccgcgta ccggaacgtg gagctgccgc tcctcctcac gcgcctcagc
     4201 gcgtccgagc ggaagaagcg ggtcgagctc gcgctctcgc tcgtcggtct cgagggacgc
     4261 gacgggcact acccgcgcca gctctcggga ggcgaggagc agcgcgtcgc gatcgcgcgc
     4321 gcggtggtcg ccgacccgac gctcctcgtc gcggacgagc cgaccggcga tctcgactcg
     4381 aagaacgccg aggagatcct cgttctcctc cgccgcctca acaaggagct cgggaagacg
     4441 atcctcatgg tgacgcacga tccgcacgct gcggaaaccg ccgacaagac gctccatctg
     4501 gacaagggag tgctccggtc gtgaagtacc tccgctacgt cctctcacat ctctggcagt
     4561 cgaagcggcg gacgttcctc acggtctcct cgatcgcggt cgcgctcttc ctcttttgca
     4621 cgctccggac cgtgatgacc tcgctcgacg cggggctccg cgcgtccgac gacacgcgcc
     4681 tcgtggtgcg ccacgccgcg tcgctcgtgt ttccgctccc gctggcgtac cgggaccggc
     4741 tggcccaggt gccgggcgtc gtgaaggtcg gcttcgggaa ctggttcggc ggctactacc
     4801 aggacgagaa gaaccagttc acgcagttcg ccgtcgacgt gcccacggtc ttcgatctct
     4861 atcccgagat cctcctcggc cccgcggaga aggaagcatt ccagcgcgag cggaccgcct
     4921 gcatcgcggg ggaggcgctc gtgaagaagt acggctggaa gctgggagac aagattccga
     4981 tcacggggac gatctacccg ggcgagtggc cgctcatcct gcgcggcatc tatcgcgcgg
     5041 cgacgccgga cgcagacgag aacgcgatct tcttccactg ggacttcctg aacgagtcga
     5101 tgcccgaagg ccggaagaac ctggtcggaa tctactggct caaggtggcg gccccgtccg
     5161 acgcgccgcg cgtgagcgcc gccgtcgacc agatctacga gaacagcccc gtgccgacga
     5221 agaccgagac cgagaaggcc ttccaggcgg gcttcatcca gatgatgggg aacgtgagca
     5281 cgctgctcac gatcctcggc tcggcgatca tcttcgcgat tctcctcgtc accgtgaaca
     5341 cgatgatgat ggcggcgcgc gagaggacga ccgagatcgc ggtcctgaag acgctcgggt
     5401 tccgggacgg tctcgtgctc cagctcgtgg ccgccgaggc gatgctcctt tcgctcgcgg
     5461 ggggcatcat cggatgcggc ctcgcattcc tcctcttccg caaggtgaac ttcaccgcgg
     5521 gaggcatgat cccgaacttc cgcgtgctgc cggagaccct cctgctcggg atcctcctcg
     5581 cgatcgcgat ggggctcctg agcgggctcg tgccggcgtt cagcgcggcg cggctgccga
     5641 tcgcgtcctc gcttcgaaag gtcgcctgat ggcccgcatc ccgctcgcct acaacctccg
     5701 caacctggcc gcgcgccggg tctcgaccgg gctcaccgcg ctcggcatcg cgctcgtcgc
     5761 ctgggtgttc atcttcacgc tcgcgctcgc cggcggattc caggccgcgc tcacgaccac
     5821 cggcaccaag gagaacgcga tcatcatccg gaagggctcg acgagcgagc tcatgagcat
     5881 cctgacgcgg gaagcggccg gcgccatcga gagccagggg gagatcgccc gcgcgcccga
     5941 cgggacgccg ctcgcggcgt cggagctgat ggtggtctgg aatctccccc ggaagtcggg
     6001 cacgacgacg aacgtggtgg tgcgcggcgt gggtccgaag tccttcgccc tccgcccgca
     6061 ggtgaagctc atccgggggc gcatgttcca gcaggggctc gacgagatca tggtgggacg
     6121 catggtggcc gaccgcttcc agcactgcga cgtgggggat cagctcaagc tcgcggggcg
     6181 cgagtggacg gtcgtgggcg tgttcgactc caacgggaga acgtacgact ccgagatctg
     6241 gggagacgtg gagcttttca tgccggtctt cgatcgtccc gtctaccaga gtctcacgct
     6301 ccgtctggcg gagcccgggt acctggcccc cctgaagaag cggctcgagt ccgatcccag
     6361 gatgaccgtc gaggtggagc gcgaggacaa gttctacgcc aagcagtcag ggcagctctc
     6421 gtcgatgctc cgggtgctcg ggctcttcgt gacctcgatc atgtcgctcg gcgcgatctt
     6481 cggcgcgctc aacacgatgt acgcggccgt cggcaaccgc acgcgcgaga tcggcacgct
     6541 ccgcgcgatc ggcttctcgc gcggcgccat cctcacgtcg tttctcttcg agtcggtgct
     6601 cttagcactc ctgggaggcg cgctcggctg cctgctcgcg ctcccggtgc ggagcttcac
     6661 gaccggaacc atgagcttcg ccacgttctc cgagctcgcc ttccgcttcc aggtgacgcc
     6721 cgggatgctc ctcgcggggc tcgccttctc cgcgttcatg ggactcctcg gcggcttcct
     6781 gcccgcgcgc aaggcggcga cgatgcccat cgtcgactcg ctccggcaag cgtagcgatg
     6841 cgctcgctcc ggcgacgggc tccggttctt taccgcgcga agggcgctgt gctagcgtcg
     6901 gccgcctcat gagccacgcc gtctcatcgc tcaagctggg cgtccgtccc agccgcctcg
     6961 gccgctgtct cggcgagcgc atcgccgagc aggcgcgcga gcacctgtcg ggtcccgtcg
     7021 cgttcacgcc ggtcgccgaa gacgtcgacc cgacgggggc cgtcttcggc tggccgggct
     7081 cgtcgctcga gcgcgcgctc ctctcgggtc aggtggacgc ggccgtcctc tccgtgaagg
     7141 acatctcgcc cgatctcgat ccggccctca cgatcgccgc cgtcaccgag cgcttcacgc
     7201 cgttcgacgt gttcatcgcg cgcgacgaga cgatcctgga cgagcttccc gacggcgccg
     7261 agctctccgc gcacacgtcc atccggcgcg cgcagctcct ccgctaccgg gaggacttcc
     7321 agctcctcga cgtgcccggg agcctcgacg accggatccg gatcctggac gccggcggac
     7381 tcgacgggat cgtggtgtcc gcctccgccg tggagcatct cggctatcag gatcgggtga
     7441 acgagatctt cacgaccgag ctgatccttc ccgcgccggg acagggagcc tgcgccgtcc
     7501 tcacgcggag cgacgacaag gagcgcctga agctgttccg gcctctcgac catcccatgg
     7561 cccgggccga ggtggagtgc gagcgcgcct ttctgctcga gctgggcgcg gagccgggct
     7621 ccgctgtcgg cgcgctcgcc gcgttcgacg gcacgtccat ccgactggag ggggcggtgg
     7681 ccgacgagac ggggaagagg ctcgtccgcg acgcggagga aggcgagtcg ggcgaggagg
     7741 cggagatcgg caggagactc gcgcgccggc tcattgtcga aggcgcccgg cgcatcatcg
     7801 ccgcggccgc ccggtagccc atgctcgatc tcaagcgcgt ccgcaccgag accgaccgcg
     7861 tgcgcgaggc ggccgggtgg aagaagtgcc aggtcgatct cgaccggctc ctccacctgg
     7921 acgaggagcg ccgcgcgctc atcatgaagt ccgaggccct gaaggcggag cggaacaagg
     7981 cctcggagga gatcggcaag aagaagaagg ccggccagga cgccgccgac gccgtggccg
     8041 ccatgaagcg ggtcggggac gagatcaagg atctcgacgg gacgctcgcc gagctcgacc
     8101 aggcgatcga gtcgatccag atctggattc ccaacgtgcc tcacgaatcc gcgccgcgcg
     8161 ccgccgacgc gacgggaaac caggtcgtcg cgagctgggg cgagattccc gcgttcgcct
     8221 tcgagccgaa gccccactgg gagctggccg aatcgctcgg gctcctcgat ttcacgcgcg
     8281 ccgcgaagat cgcggggagc ggcttccttc tcttcaccgg gaagggcgcc cggctcgagc
     8341 gcgcgctgat ccagttcatg ctcgacctcc acacccgcaa gcacggctac gtcgagctct
     8401 cgccgccgca tctcatccgg cgcgactgcc tcttcggaac ggggcagatt ccgaagatgg
     8461 aggacgacat gtaccggctc ctgggggagg atctcttcct caacccgacg gccgaagtcc
     8521 cggtcacgaa cgtctaccgg gacgagatcc tcgagggcga gcgtcttccg atctaccaca
     8581 cggcctactg cgcgagctac cgcaaggagt cgggggccgc ggggcgcgac acgcggggga
     8641 tgctccgcgt ccaccagttc gacaaggtgg agctggtcaa gttcgtgcgc cccgagacgt
     8701 cgtacgacga gctcgaatcc cttcgcgcgg acgccgaggc cgtgctccgg gcgctcgagc
     8761 tcccgtaccg ggtgctcctc ctcgcggcgg gcgatctgag cttcgccgcc gcgaagtgct
     8821 acgacctcga agcctgggcc ccgggcgaga agcgctggct ggaagtgtcc tcgtgcagca
     8881 acttcgagga cttccaggcg cgccgcgcgg ggatccggtt ccgccccgag cgcggagcca
     8941 aacccgagta cgtgcacacg ctgaacgcgt cgggcgtggc gctcccgcgc acctatgcgg
     9001 ccctcctcga gacacatcag accgagcggg ggaccgtgcg cgttcccgag gcgctcgtgc
     9061 cctacctcga cggggtccgc gagatcaccc ccgcgtaggg gacgcgatgc ccgcgctccc
     9121 cacgcgccga ctttcgtacc gcatctggtt cttcctcgcc gtcctcgtcc tcatgctcgt
     9181 gctcgtcgcg cactcgaacg cgatgatccg gcggctcaac gaggagacgc gcgcacggtg
     9241 cgacgtgctg gcccgcttct tcgccgtggc cacgttccag gcggtggagg accccgcggt
     9301 gcgtcccatc tttcgcgacg tggtgcggag catcgacttc ccggtcgtgc tcaccgacta
     9361 tgccgggata ccgcgcgcgt ggaaggagat cggcatcgcg ccggacgcga tccccgattc
     9421 cgtgatcgag cgcgccgccg cgaccggcgt aatcccgccg gaggtggagc ggatccagca
     9481 catcgtggag cggctcgacc gcatccatca gccggtcgac atcgtggaag tcgggaagaa
     9541 cggagcgctc ggcaaggtgc actacggcga gcccaacctc gtgaaggagc tccgctggct
     9601 cccgtacctc gagctcttcg tgatcctcgt gctcctcctc ttcggctaca ccgggctccg
     9661 gagcctcatg acggcggagc agcgctcgct ctggaccgcg ctcgcaaagg agaccgcgca
     9721 ccagctcggg acgccgctct cgtcgttgat ggggtggacc tcgctgcttc gcgagagccc
     9781 gccggagcgg ctcacgccgg agcgcacccg cgacatcctg gacgagatgg accgcgatct
     9841 cgcgcggctc cagaaggtga cgctccgctt cgggcaggtc ggctcgctcc ccgtcctcaa
     9901 ggagggcgac ctggtcgaga tcacggcggg gaccgtcgac tacttccgca cgcgcctgcc
     9961 gcatctcggc cacgcgatcc ggatcgagga acgctacgag cccgtgccgc gcgtccgtgt
    10021 gcaccgcgag ctcttcgagt gggtggtgga gaacctgctt cgaaacgcgc tggacgcgat
    10081 cgacaggccg gacggttgga tcgaggtgac ggtgcgctgg ctgagggagg agcggacggt
    10141 ggagatgatc gtgcgcgaca acggccgcgg catgacgctg gacgagcggc ggcgcgcgtt
    10201 cacccccggc ttcacgacca agaggcgcgg ctgggggctg gggctcgcgc ttgcgaggcg
    10261 cgtggtcgcc gagtaccatg gagggcgcat ctccatcgtg gagagcgtgc ccggccgcgg
    10321 gaccgcggtg gccgtggccc tgcccgtacc gcctcccccc gactcccgcg cgacgaaacc
    10381 aagcgaagga cagagcgcgt catgaacgtc acgcctcgaa aggtcctctg ggccgacgac
    10441 gagatcgatc tcctgaagcc ccacatcctc ctgctcgagg agaaggggtt cctggtgacg
    10501 ccggtctcga acgggcacga tgccgtggcc tgcgcctcgc gcgagaagtt cgacgcggtg
    10561 ctcctcgacg agtccatgcc cgggatgggc ggtctcaaga cgctcgaggc gatcaaggag
    10621 aaggacccca cggtccccgt cgtcctcgtg accaagaacg aggccgagtc gctcatggac
    10681 gatgcgatcg gccggcgcat cgacgactac ctcctcaagc cggtcctgcc gcagcagatc
    10741 ttcctcgcgt tgaagcgtct cctcgagtcg gaccggcttc aagagagccg ccgcgcgcgc
    10801 gactacgtcg cggagctggc gcggctccgc gtggagtccc cgagcacgct cgactggcgc
    10861 ggctggatcg acctctacgc gcggagcgcg cagtgggacg tggagctgga ccaggtcgac
    10921 gatccggggc tcaagcaggc gcagggcgat cagcggcggg agctcaacct cgagttctcg
    10981 agccacgtgg agaggtccta cgagagctgg ctctcgggga ccgatcggcc ggccctgtcg
    11041 acggacatcg tggagcggtg ggtggcgccc cacttgcgcg aaggaaagcg cgtcgcgctc
    11101 atcgtcatcg actgcatgcg gctcgaccag tggctcagga tcgagcccct gctccgcccg
    11161 ctctacgaca tcaccgtcga ccaccagctc tcgatcctgc cgagcgcgac gccgtactcg
    11221 cggaacgcca tcttctcggg actctttccc gcggagatcc agaaggagca tcccgagttc
    11281 tggcaggaga cgacgtccga cgagcggagc aagaaccgat tcgagcggca gctcctcgag
    11341 caccagctcg cgcggatcgg ggtcaagctt ccgtcggggc tcaagtacct caaggtctac
    11401 aacaccgacg aggccgccaa cgtgcggcgg aacgtggtca cgtaccagaa tatcccgctc
    11461 gtcgcgatgg tgttcaactt cctcgacatg ctcacgcacg gacgctcgga gtccgacctg
    11521 ctccaagagc tggcgcccga cgagtccgcg ttccggtcgg tcatgaattc gtggttccgg
    11581 cattcggcgc tgttcgacgc gctcaaggtg atggcgcggc aggaccaggt cgtggtggtg
    11641 acgtcggacc acggcgcgat cctgagccgc cgctccgctc tcgtgtacgg aaaccgcgag
    11701 acctcgacga atctgcgctt caagtacggg acgaatctcg gatccgatcc caagcaggcg
    11761 gtccagatca aggacccggc gcggttcaag ctgccggcgg accagccgca caagcagtac
    11821 atcctcgcga aggaagactt ctatttcatc tatcccacca agttccacga gtacgagagc
    11881 aagtaccgcg ggagcttcca gcatggcggc atctcgctcg aggagatgat cctccccgtg
    11941 gcgacgctcg tgccgaaggg ctgaaatgga cgggatggag atcgagcttc gctcgctctt
    12001 gcgcaccgaa ggcgagacgc gctccttcgg gcgccaccta ggtacgctcc tccggcccgg
    12061 cgactgggtc gcgctcaccg gagatctcgg atcggggaag acgacgctcg tctcgggaat
    12121 attagagtca ctggctcccg gagcgcgtgg acggagcccg acctacgtgc tcgtcgaggt
    12181 gcatcccggt cccgtaccgg tcgtgcacgc cgatctctac cggctccgct cgctcagcga
    12241 gctccccggg ctcgcgctcg acgatctcgc cgagccggga gcggtggtcc tgatcgagtg
    12301 ggccgaccgg gcggagggga atctgcccgc ggtcaggctc gatctcgcgc tacgctatct
    12361 ggacggaccg gggcgcgagc tgcgcgtccg cccccacggc gcgcgctgga accaggcggc
    12421 gcgcgacggg ctcttcgacc gcgatcggtg gcaacatgtc ctacatcctg ggaattgata
    12481 cgtcggcccc cgagggctcg gtcgcgctcg cgcgggacgg acgcgggggc gcgatcgaga
    12541 tgctccctcc gggcggacat tcgagcggcc tttcggcggc ggtcgggcgc ctgctcgagg
    12601 cagcgggaat cggcctcgat ggcgtggccg cgttcgcggt gaacgagggg cccggttcct
    12661 tcaccggact tcgaatcgga ctcgcgtggg cgaaaggggc ggcgctggga tccaaccgcc
    12721 ccctcgttct cgtgcgcgcg cacgaggcga tggcgctcgc gcacgccgcg gacgcggagc
    12781 gcctcgtcac ggcgattccc ggcgcgcggg gcctggtcga ggcggcgctc tgggagaagg
    12841 gcgctgcctc gtgggggccc gacacggtcg aagagggtga tctgattccg acgctcctcg
    12901 agcgcggcac gcagggactc ggcatcgtgc ccgcgacggc gaagctcgcc cggtcgctcg
    12961 ccgaggaggc ggcggaggag gggatcgcga tcctcgaatg gcgcccgctc gcccccgctg
    13021 tggccgcgat cggagcccgt ctcctcgacc aaggtcaggc ggccgacctc acgctggcgt
    13081 cgcccgcgta tggacgcgag ccgaacgcga gaaagccggg atcgtgaacg cgtccccggc
    13141 gcccctctcc aagctcagga tcgggtccct ctcggtcgcg cgcatcgacg aggtgctcga
    13201 gatcgagaag ctctccttca ccgatccatg gagccgcgag atgttccgct ccgagctcga
    13261 ggtgggaggc ggaacgtacg cgcgaatggc cgagcgggat gggcgccttc tggggtattc
    13321 cctggcggtc ctcgtggcgg acgaggcgca cctcgggaac ctggcggtgc accccgacgc
    13381 gcgcggggcg ggcgtgggcc aggcgcttct cgacgatctg ttacaggccg ccgagaagcg
    13441 ccgggcgacg cgcgtgacgc tggaggtgcg cgagtccaat gatagagccc gtaagttcta
    13501 ttatcgaaac ggtttcatcg acgtcgcgat ccggaagaac tattatcgga accccgtgga
    13561 ggatgctatc gtgatgctcc ggagcctccg gggagggtaa cgatgacctg gctcaggaag
    13621 gcgaagaagg ggctcaagcc ccaacagcgc cggcgggagc ttcccgacgg gctgtggacg
    13681 aagtgcgagg attgcggcga gatcctgtat cacaaggagc tcgagcgcaa tctctggacc
    13741 tgcagcaagt gctcctatca cttccggatc tccgcgcgcg cctacatcgc gatcctggtc
    13801 gatgagggga cgttcgtcga gcgcttctcc gaggtcgtct ccaccgatcc gctcaagttc
    13861 aaggacgcga agcgctatag cgaccggctg aagcgcgcgc gcgaggagac cggcctctcc
    13921 gaagcggtca tctgcggcga ggggcggatc gaagagcatc cctgcgtgct cgcgatcatg
    13981 gacttcgcgt ttctcggagg gagcctcgcg tccgccgcgg gagagcgcat cgcgcgctcc
    14041 atcctcctcg cgatcgagac gcggcggccg ctcatcatcc tctcgtcctc cggcggcgcg
    14101 cgcatgttcg aggggatcct ctcgctgatg cagatggcga agacgaacgc gctcctggcc
    14161 cggctctccg acgcaggcgt cccctacatc tcgatcatga cccacccgac gacgggcgga
    14221 gtcacggcga gcttcgcgtc ggtgggagac gtcatcctcg ccgagccgaa ggcgctgatc
    14281 ggattcgcgg ggccgcgcgt catcaagcag acgatcaacc aggagctccc ggaaggattc
    14341 cagcggtccg agttcctgct cgagaagggc atgatcgacc gcatcgtgca ccggaccgag
    14401 ctcaagaaga cggtcgcctg gctgctgcgc ttcttcgacg cggcgagcgc atcgctgccg
    14461 cacagcccgc gccgtcgcgc cggcgaatcg ctggagcgct tccgcgccgc gtcgcttccg
    14521 atcggaaccg cgcttccgcc gtcacctccc gcccccgcgg gccagggcac ctcgcagccc
    14581 ggcgccaacg gatcggcccc agaaagcgcg acgcgggacg ccgcggcttc gcgcgacacc
    14641 actgccgaac gggaagtgcc catccaggaa tgaaggtcac cgagggcgct tccctgacga
    14701 agcgcctcga cgagctctac cgcttcgagc ggagcgcgat gcgccccggg ctcgccgggg
    14761 tggagcggct cctcgcgcgc gccgggcgtc ccgatctcgc gttcccgtcc atcctgatcg
    14821 cgggcacgaa cggcaagggc tcgaccgcgg cgcacctgga ctcgatcctg cgcgccgcgg
    14881 ggctcaagac cggcctctac acctcgccgc acttggtccg cttcggcgag cggatccggg
    14941 tcgggggccg ggagatctcc gagccggagc tcgaagagct cctcgcgacc tggtggccgc
    15001 ggttcgaagc ggagaatccg tccttcttcg aggcggcgac cgcgctctgc ttcgatcact
    15061 tcgcccggag cgggatcgac atggccgtcg tggaggtggg cctcgggggg cggctcgatg
    15121 cgacgaacat cctcgagccg cgcgtcgcga tcatcacgac gatcgcgaac gaccatgcgg
    15181 acattctcgg cccgacccta cgtcggattg cgggggagaa ggcgggaata ttgaagaaag
    15241 gcggccgcct cgtcctcggc gtgcgtgcgg gagagccgcg cgacgtgatc ctcgccgcgg
    15301 cccgggagct cgacataccg gtgacgctcc tgggcaagga cggaagctac ggctcgacgc
    15361 ggctcacccc cgagggcacg gagttccatc tcagaacccg caccctccgg gcgcgcgtgg
    15421 gtacgccgct cctggggcgg caccaggctc ggaacgcggc gctcgccgct ctcgcggcgg
    15481 agatcgcgct ccagggaatg ccggatccct cgatcgcgcg cgccatcgac tcggggctca
    15541 ggaacacgcg gtggcccgcg cgcgccgaga tccttcccgg caatccgccg atcttgatcg
    15601 acgtcgcgca caatctcgag ggagcgatgg cgctcgtcga gacggtgcac gccctctggc
    15661 ccgcgcggcc cctcgcggtg gtcgcgggct tctcgcgcga caaggaacac gaggcgatcc
    15721 tcgccacgct cgcgcgcgtg gggtcgcgct tctatctcac gccgttcgag ggcgagcggg
    15781 ccgccccgat ggagcgcgtg ctcgaagcgg cgcgtgccgc cggggtcgcg ttcgattcgg
    15841 tgcccgacgt caccgtggcg ctggcgctcg cgaaggcgtg ggcgaaggag cggaagggag
    15901 cggtgctgct cgcggggtcg gtctacctcg cgggagcggc catgccggcg ctcgcgctcg
    15961 aggtgccgca tgcgctctag aggagcgccg ctcctcgccg cgatcgccgt gctcttcgcc
    16021 gctctcgctg tgggggcggc gctcgcacag cctcctcctc ccggcaccgt ggccccgggc
    16081 ggcaaggacg acgagcccta ccggctcacc gccgaccggc tcgagggatc cgcgtccgcg
    16141 ggggagaacg tgtacaccgc gatccgcccg accgtggtgc acggcacgac caccgtgacc
    16201 ggcgattccg cgctgatcta caagaaccgc gagctcgtgc tgtttcgcgg gaacgtgaag
    16261 atcgtggacg ggaccacgca catgtggggg cgcgaagcgt cctacgatcg gaagacccgc
    16321 ctcgccacgc tgcgcggcaa cgtgcggatc gaggagggga gcgccaagat cacgggcacc
    16381 gaggcccggt tctatcgcac cgagaaccgg tcggtgatca ccgggaaccc gcgcctcgaa
    16441 gactcgaccc gcacgctgac ggccgaccgg atcgagtacg accgcacgaa cgacgtcgcc
    16501 gtcgccatcg gcaaggtgga cgcgatcgac cgcgccgaga gcacgcgcgt gcgcgccggc
    16561 cgcgtgcgct acgaccgacg cgccgactac gcgtgggcca ccgaggaccc cgttctcgtg
    16621 cgcgaggagc ggaagggcca gcacaccacc gtgcggggcg acacgctcga gttcgacaac
    16681 cccaaccgcc gcgcctacgc catcggaaac gtgcgcgtgg agcgcgagaa gctccgggcc
    16741 acggggacgc gcgcggagtt cgatcaggcc caggaccgcg cgatcctcgt ggggaagccg
    16801 cgagcgtggg acgaccaggg cgtggcgtac ggcgacacga tccagatcga gttcgaggac
    16861 aacgcgctcc gcacgctccg catccacccg catgcggtgg tcgagttcac ggcgaaagcc
    16921 gattcggggc gcggcgagcg gaacgtcgcg agcggcgaca cgatcacggt ccacttcgag
    16981 aaggaggaag cgcgcgaagc gctcatggtg ggccacgcgg agagccggta ctggccgagc
    17041 agcgccgaca gctccgaagg cggccggaac gtctcgaccg gcgattccat ccgcgttttc
    17101 ttcgacaagg gtgagcccgc gcgcgccacc gtgcgcggcg gcggccgcgg cacgtacttc
    17161 ctcgccgcgg agggggacac ggccagggaa cggcagcagg atctcgtgaa gtatgagggg
    17221 gacgagatcc agtacgacat caagaagggg accgtggaca tcgtcgggag ctccgacgtc
    17281 acctatcgcg aggtccacct gacggcggac aaggtcacgt tcgactcgaa cacggaccgc
    17341 atgcgcgcgg aagggaagcc cgttctcacc gactccggaa accggatgac cggagcctcg
    17401 atgacctacg acctgaacac gaagcagggg accgtgttcg aggggcggac cgcgtacgac
    17461 cggggctttc tcttcgggga gcgcgtgcgg cgcgtctcgg acaccgagct ggacgtcgcg
    17521 cacggcacct actccacgtg cgacctggcc gagccgcact tccacttcga gagcgcgaag
    17581 atgaaggtga tcctccacga caaggtggtg gcccgcccgg tcgtcttcta catcaagaag
    17641 gtcccggtgc tcgccctccc gttctacgtg ttcccgatcc agaccggacg ccactccggc
    17701 ttccagctgc cgcaggtgga attcggatcg gcgacgggag gcgggaagtt cgtccggaac
    17761 gtgggctact actgggcgat caacgacttc atggatgcga ccggctggtt cgactactac
    17821 cagcaggaca gctggatcgg gcacggcctc taccgctaca accaccgcta cgagtaccag
    17881 gggcagatca gcgcgtccta ccagaaccag ttctcgacgg gctcgaaccg ctgggatctg
    17941 ttcgctcgcc actaccagat cctcgggcgc gccttcacgc tcacgagcga ggccaacctc
    18001 accaactcga gcgcctatcg ccgggatcag aacctggggc agaacgtgtt cgtccgcacc
    18061 cagcggaacc tccggtcgaa cctgtcgctc cagaagggat ggagcggcgg ctcgttcgcc
    18121 ctcggtttgc tccagaccga ggacctggac gccgagccgg gaaatctcaa gaccgagcag
    18181 cagctgccgt ccttcacgct caccctgaac gcgcgtccgc tggggcacgc ggcgcggggc
    18241 cgcgagccgg cgaagtggcc cgccttcatg agcacggtct acagcaccag cctccggggc
    18301 ctctaccaga ggcgggagta cgagcccacc acgcaccccg acacgacggt cgtcttcgac
    18361 agcctcgccg tgccgatcga cacgacgatc accaccgtga gcgtggactc gctcgacgcc
    18421 cgcggcgcgg cccgctacga cttcacgctc accgacgtgc gctccctcgg cttcatcaag
    18481 gtggctcccc gcttcaattt cgccgcggtc tactacagtc gcgaccaggc ggggcaggag
    18541 aaccaggtcg gcacggtctg gggcgccggg atcggagcca ataccgcggt cacggggacc
    18601 ttccgcacgg ggctcggtcc gctccaggcg atccggcatg tggtcacgcc gtcggccgcc
    18661 ctcgtctacc agccgccccg aacgagcctt ctctatcgag gcccggacgg gttcctccgc
    18721 ccccgcttcg acggcgtgtc cggcatctcc ctcgcggcgg ccgaggtgcg gcgtctcgat
    18781 ttctcgctcc ggcaggatct ccacgtgaaa tggggggatc ccatccatcc gcacgtgatc
    18841 aacaacctgt tccagctcca gaacaacatc tcgtacaacg agctcgccaa gcgctccggc
    18901 gcgaaaccgc tctccgacat gtcgacgagc ctcaccttca agccgatcga tcggagcgac
    18961 ttcgcgttca acttcgtgca caacccgtac gactggaagc tcctcgcctt cagcgcgagc
    19021 accggcatcg cgctccaggg gcagagcccg acgggggagg agaactcgat cgccgaccag
    19081 gagccgggca cctacgccgt ccgcgagcgc tcgccgctca ccccggtcgg catgtccccg
    19141 gccggacttc cctggagcgt gttcgcatcg atcagctacc aggggtcgag cgcccgcgac
    19201 gggcgggggg gctacaaccc gtggcagtcg accgcccgtc tcaacggcgg gacggcggtc
    19261 aacttcacga agaactggcg gttcgagtac aactggcagt tcgatccgcg cgcgggagtc
    19321 atggtctccc agttcttcac cgtgaagcgg gacctccact gctgggagat gcagttcacg
    19381 cgaagtcttt caggcgacgt gggagacgag tattacttca agatcaacgt gaagaatctg
    19441 ccggaggtct acttcgagca agggtcgcgc ggcctgcgcg gattcggcgc gctccagaac
    19501 atctattgat cgaaaaacag gcgtcctcat gcggaatctg tttgacaccc cctggggccg
    19561 gtcctaaggt gacgcggcgt tcatccgaaa ggaggtgaaa cggaatgaac aagaccgagc
    19621 tcatcgccgt catcgcaaag aagacctccc tctccgcacg cgaggcgcgg gccgtcgtcg
    19681 acacgctctt ttctcccggc aaggaaggtt tgatcgcgtc ggcgctcatg tccggcaaga
    19741 aggtccagat cacgggattc ggtacgttcg agacccgcaa gcggaaggcg cgcgaaggtc
    19801 gcaatcccca gacgggcgag cggatcaaga tcgccgcgtc gagattcccg gcgttccacg
    19861 cgggcaaggc gctcaaggat cgggttcgga agtaatcccc cccaaaggct atcgacggcg
    19921 ggcgggcccc cagggtccgc ccccttcgtt ttttgggtcc ccgggcccca gaactcgggc
    19981 tcggcgttga acctgccgaa gaagccgggg tatagttccc cgacacccag cgcgttcact
    20041 tcggtcccga agggaggtgg cttctctgcc gcgtctccgc tacctgttct tacttgttgc
    20101 agcgcttata cttgcaaccg caccactgct ccagggaaag ggcgccccgg tcgacatcct
    20161 cattcagagc acgaccgacg tctacggtga gctcgctccc tgtggctgac acgctcatcc
    20221 gctaggcggg ctcgcccgga gggcgagcca actcaaggtg ggcgagctga agttccccaa
    20281 caacttcacg atcgactgcg gcgatttctt cggcgtcggc ggcgagcagg actcgctgaa
    20341 gagcgcgttc ctggtccacg ccatggacaa gctcggctat gacgtcgtga cgctcggcga
    20401 gcgggagttc aacttcggtc agcagttcct gctcagccag ttcaagacga ccaaggtgga
    20461 cgtcgtgtgc gcgaacctgg tctatgcgga ctcgaagaag ccgttcgtga agccgtacgt
    20521 catccgcaag gtcggcaccc tcaaggtcgc gttcttcggg ctcttgggga aggacatgaa
    20581 gatccggccg ttcaagggag acccggcgct cacgatcctc gatccgaccg aaaccggaaa
    20641 ggcgatgctc gccgatcttc ggaagaaggc ggacatcgtg gtcctcctct cgcatctcgg
    20701 gatgagcgac agccagaagc tcacggtcga cgtgccggtc gacgtcatgt tcttcggcca
    20761 ccagccgggg ctcttccgga cgctggcgaa gacgcaggac gtgatcacgg tgcggggcgg
    20821 cgagcggggc cagtacatcc ctgaagtgca cctcgtcgtg gaggacaaga agatcacctc
    20881 cttcgacggc gacgtcgtgc agctcgatga caaggtcccg gcggacgaag cgatgaacgc
    20941 cgaggtcgac aagtaccagg acgcgctcaa ccagcggttc gcgaaggcca acgaggccac
    21001 cgcgcagacc gcgacggcgc acaccgcgca gcagatcgcg ggcgagcgct acctgggcga
    21061 gaacaactgc cgccgctgcc acgaggccga gtatcagaaa tggtccacgc agaagcacgc
    21121 gcatgcgttc cagacgctca tcacgaacca gcgcgactcg acgcccgaat gcctgccgtg
    21181 ccacgtcgtg ggcatgggct cggccggcgg gttcatctcc aagcagacga cgcccgatct
    21241 ggtgaacgtc cagtgcgaga gctgccacgg catgggcacg cgccacggta tggccgacac
    21301 cgtcggtccc gaggtctgcc agacctgcca tacccacgag caggatccga atttccagta
    21361 tgacgagatg gtccagaaga tcgtccattg ggagtgaccc gatgaggtcc gcgatccgcc
    21421 gcgggccttc ggcgatcctc gttctcctcg tttccttcgt ttccatcctg ggcgcgccgg
    21481 cgaagccggc gcgcgccccg gc
//
LOCUS       DASTKC010000107         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_789931, whole genome shotgun sequence.
ACCESSION   DASTKC010000107 DASTKC010000000
VERSION     DASTKC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789931"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2060)
                     /locus_tag="VFR25_04490"
     CDS             complement(<1..>2060)
                     /locus_tag="VFR25_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9940336.1"
                     /translation="QLSAYHEFGRGPVPGLVREKARSAPELAEVIHDLSVIEKHRAFV
                     DLMMSALFPPAFWEQEYGAALFPFELRAFYATPPFRRSLMNADGTLQGRANFLQEHSV
                     EQTLAAARLLLAYELILERTYGIDLGVDVPIIFTTADPATGLDQHFRLQFDWRFVDVE
                     LVGPKPPLSDAMRQRLQAGTIDADLLREHLPPDRFVLRGFTVVKAVDVTDQEVLSSLK
                     RDLIDKDSIVSSSHFQGLQAKLRTFFRRPDLELGLAAVEGDRVLVLNDASRHEQACIF
                     ADSAHHTTSEFKGSLYERAFTQGRPIVVDDLRAYPGRTLIEEELIQNGVRNFVCAPLH
                     YQDKVIGTLELVSARVGDLNATHLPKLQEVLPLFSMAVQRSVEELNSRIQTVINEQCT
                     AIHPTVEWRFRRAVLNSFERQRGTVATVTELEPIIFEGVYPLYGLADIRGSSTQRGEA
                     IQADLLAQLRLAQAVVQAASEVQFLPALDELAYRIDTRTAQIERTLNSSDEIGIIAFL
                     RTEVESLLDHLGTFGPGVRDRIATYRAGLDARLGAVYHRRRLFEDSVTGIAESISSYL
                     DLEQQAAQRMFPHYFEKQKTDGVDYQMYVGAGLLENGRVDPLCLKNLRLWQLMVTCGI
                     AVRAHQLRDRLPIPLETTHLILVQHAPLSIRFRFDEKRFDVDGAYDIRYEIVKKRIDK
                     AVVQG"
BASE COUNT          354 a          644 c          725 g          337 t
ORIGIN      
        1 ccctggacga ccgccttgtc gattcgcttc ttgacgatct cgtaccggat gtcgtaggcc
       61 ccgtccacgt cgaaccgctt ctcgtcgaac cggaaccgga tcgagagggg ggcgtgctgg
      121 acgaggatca ggtgcgtggt ctcgagcggg atcggcaggc gatcgcggag ctgatgggcg
      181 cgcaccgcga tcccgcacgt gaccatgagc tgccacaggc gcaggttctt caagcacagg
      241 gggtcaacgc gaccgttctc caggaggccg gccccgacgt acatctggta gtcaacgccg
      301 tcggtctttt gcttctcgaa atagtgcggg aacatccgct gcgccgcctg ttgctcgagg
      361 tccaggtacg acgagatgct ttccgcgatg ccggtgacgc tgtcctcgaa cagccggcgc
      421 cgatggtaga cggcaccgag ccgagcatcg aggccggccc ggtacgtcgc gatccggtcg
      481 cggacgccgg ggccaaacgt gccgagatga tccagcaggc tctcgacctc ggtccgcagg
      541 aaggcgatga tcccgatctc gtcgctggag ttgagcgtgc gttcgatctg ggcggtgcgg
      601 gtgtcgatcc ggtaggcgag ctcgtccaag gccggcaaga actgcacctc gctcgcggcc
      661 tggaccaccg cctgggcgag gcgaagctgg gcgaggagat cggcctggat cgcctccccg
      721 cgctgggtcg acgagccgcg gatgtccgcg agcccgtaga gcgggtagac accctcgaag
      781 atgatcggct ccagctcggt caccgtggca acggtgcccc gctgccgctc gaagctgttg
      841 agcacggctc gccggaatcg ccactcgacg gtggggtgaa tcgcggtgca ctgctcgttg
      901 atgacggtct ggatgcggct gttcagctcc tccacgctcc gctggaccgc catggagaac
      961 agcggcagca cttcctggag cttggggagg tgggttgcgt tcagatcccc gacgcgggcc
     1021 gagaccagct cgagggtgcc gatcaccttg tcctggtagt ggagaggagc acagacgaag
     1081 ttccggaccc cgttctggat cagctcctcc tcgatcaagg tccgaccggg gtacgcccgg
     1141 aggtcgtcga cgacgatggg gcgaccctgg gtgaacgccc gctcgtagag agaccccttg
     1201 aactccgacg tcgtgtggtg ggcggagtcg gcgaagatgc acgcctgctc gtggcgcgac
     1261 gcgtcgttca agacgagcac acggtctccc tccaccgccg cgaggccgag ctccaggtcg
     1321 ggccgccgga agaacgtccg gagcttcgcc tgcagcccct ggaagtgtga gctcgacacg
     1381 atcgagtcct tgtcgatgag gtcgcgcttg agcgaggaca ggacctcctg gtcggtgacg
     1441 tccacggctt tcacgaccgt gaagccacgg aggacgaacc gatcgggcgg caggtgctcg
     1501 cgaaggaggt cagcgtcgat cgtgccggcc tgcagccgct gtcgcatcgc atccgaaagc
     1561 ggcggcttgg ggccgacgag ctcgacatcc acgaagcgcc agtcgaactg cagccggaag
     1621 tgctgatcca gtcccgtggc ggggtcggcg gtcgtgaaga tgatggggac gtcgacaccg
     1681 aggtcgatgc cgtacgttcg ctcgagaatg agctcgtagg ccagcaagag tcgggcggcg
     1741 gccagcgtct gctcgacgct gtgctcttgc aagaagttcg cccggccctg gagcgttccg
     1801 tccgcattca tgagcgagcg ccgaaaggga ggcgtcgcgt agaaggcgcg cagctcgaac
     1861 gggaacaggg ccgctccgta ctcttgctcc cagaaggccg ggggaaagag cgcgctcatc
     1921 atcaggtcga cgaacgcgcg atgcttctcg atgaccgaga ggtcgtggat cacctccgcc
     1981 aactcgggcg cggagcgcgc cttctcgcga acgagacccg ggaccggtcc gcggccgaac
     2041 tcatggtagg cggagagctg
//
LOCUS       DASTKC010000108         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1026480, whole genome shotgun sequence.
ACCESSION   DASTKC010000108 DASTKC010000000
VERSION     DASTKC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026480"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..>2229
                     /gene="ppk1"
                     /locus_tag="VFR25_04495"
     CDS             193..>2229
                     /gene="ppk1"
                     /locus_tag="VFR25_04495"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET9940337.1"
                     /translation="MVDGKSLEPRYLNRLMTWLGFNERVLEEAQDISNPVLERARFLG
                     IFASNMDEFFMIRVAGVRELVLEGTQLTYPDGSSPQELLARLSERARDLAARAAAVWS
                     EGIEPALRDAGVRILAPDDLEERERKGIERTFQREIFPTLTPSAVNPAFPLPHVTNLV
                     LYLAVRVLGPDGEERLAIVQLPRSAPRWIAVSTKAPRFVLLEDAIRQSVGELFEGHTL
                     VEAAAFRVTRDADFTPDDQESEDLVDAVEQVLRSRVHGDPVRLEIEDSASEPLVEELA
                     RALELDEHAVHRLPRPIDLRFLMDFARLPSLPAPRAVPWPPQPQPGIAPGEGLWAEIA
                     ERDILLFHPYESYEPVLRLLRLAAEDPAVLAIKQTLYRTSSSSEVVRSLERAAENGKQ
                     VTVLIELQARFDEERNVNWAQRLEDAGAQVLYGLAGLKMHAKALLVIRRGTDGIERYA
                     HIGTGNYNERTALDYSDLSLLTSDEDLCADLTSFFNVVTGYSEPLPWRRIAMAPLGLR
                     QRFLGLIHREMERARSGEPARIRVKVNALIDTRLVDALYEASQAGVAIDLNVRGSCLI
                     RPGVPGLSETIRVVSLVDRFLEHSRIFEFGAGRDAEVFIGSADWMPRNLDRRVELVVP
                     VLDVQQRARLGRVLDTLFADNVKARELRPDGTFARREGRRKPVRAQEAFWTEARE"
BASE COUNT          350 a          752 c          767 g          360 t
ORIGIN      
        1 gaaagggctg gcgcccagca ggatttaagc atacttaaca ccgcccatcc aacacggatc
       61 cgtgcatggg ccgaaggggg tagggggacg ggcctcttga atcaggctcg ctttcggcgt
      121 aacctcattt gcttgttaca attggacgga actgccgtcc tcccccgctg aaggctcgga
      181 gcccgcgccc ccatggtcga cggcaagtcc ctcgaaccgc gctacctgaa ccggctcatg
      241 acctggctcg ggttcaacga gcgcgtcctc gaggaggcgc aggacatctc gaaccccgtc
      301 ctcgagcggg cgcgattcct cggcatcttc gcgtcgaaca tggacgagtt cttcatgatc
      361 cgcgtggcgg gcgtgcgcga gctcgttctc gagggaacgc agctcacgta tccggatgga
      421 tcgagtccgc aggagctcct cgcgcgcctc tccgagcgcg cgcgcgatct cgcggcgcgc
      481 gcggccgcag tctggtcgga agggatcgag cccgcactgc gcgacgccgg cgttcgcatc
      541 ctggcgccgg acgacctcga ggagcgggag cgaaagggga tcgagcgcac gttccagcgc
      601 gagatcttcc cgacgctcac cccgtccgcg gtgaatcccg cgttcccgct gccgcacgtc
      661 acgaacctcg tcctctacct cgcggtgcgc gtgctcggac cggatggcga ggagcggctc
      721 gccatcgtgc agctgccgcg ctcggcgccg cgctggatcg cggtctcgac gaaggcgccg
      781 cgcttcgtcc tcctcgagga cgccatccgc cagagcgtgg gggagctgtt cgagggacac
      841 accctcgtcg aggccgccgc gtttcgcgtc acgcgcgacg cggacttcac gccagacgat
      901 caggagtcgg aggatctcgt cgacgccgtg gagcaggtgc tccggagccg cgtgcacggg
      961 gatcccgtgc gcctcgagat cgaggactcc gcgagcgagc cgctcgtcga ggaactggcg
     1021 cgcgcgctcg agctcgacga gcacgccgtc caccgcttgc cgcgtccgat cgatctccgg
     1081 ttcctgatgg acttcgcacg cctgccgtcc ctgcccgcgc cgcgggccgt tccgtggccg
     1141 ccgcagccgc agccaggcat cgcgccgggt gaagggctct gggccgagat cgcggagcgc
     1201 gacatcctcc tctttcatcc gtacgagagc tacgagccgg tgctccggct cctccggctc
     1261 gcggccgagg atccggcggt gctcgcgatc aagcagacgc tctatcgcac gtcctcgagc
     1321 agcgaggtgg tgcgctcgct cgagcgggcg gcggagaacg ggaagcaggt cacggtgttg
     1381 atcgagctcc aggcgcggtt cgacgaagag cgaaacgtga actgggcgca gcgcctcgag
     1441 gacgcagggg cccaggtcct ctatggcctc gccggtttga agatgcacgc gaaggcgctg
     1501 ctcgtgattc gccgcggcac ggatggcatc gagcgctacg cccacatcgg gaccgggaac
     1561 tacaacgagc gaaccgcgct cgactactcg gacctgagtc tcctcacctc cgacgaggat
     1621 ctctgtgcgg atctcacctc gttcttcaat gtagtgaccg ggtacagcga gcccctgccg
     1681 tggcggcgca tcgcgatggc gccgctcgga ctcaggcagc gcttcctcgg gctcatccat
     1741 cgcgagatgg agcgggcgcg ctcgggcgag ccggcgcgca ttcgcgtgaa ggtgaacgcg
     1801 ctcatcgaca cgagattggt cgacgctctc tacgaagcat cgcaggccgg cgtggcgatc
     1861 gatctcaacg tgcgcgggtc ctgcctcatt cgccccggcg taccgggact gagcgagacg
     1921 atccgcgtgg tatcgctcgt ggatcgattc ctcgagcaca gccggatctt cgagttcggc
     1981 gcggggaggg acgcggaggt tttcatcgga agcgccgact ggatgccccg caatctcgac
     2041 cgtcgggtgg agctcgtggt tcccgtcctc gatgtgcagc agcgggcccg gctcgggcgc
     2101 gtcctggaca cgctttttgc tgacaacgtg aaggccaggg agcttcggcc cgacgggacg
     2161 ttcgcccgga gggagggccg gcggaagccc gtccgagccc aggaagcgtt ctggacggaa
     2221 gcccgggag
//
LOCUS       DASTKC010000109         6910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1737939, whole genome shotgun sequence.
ACCESSION   DASTKC010000109 DASTKC010000000
VERSION     DASTKC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737939"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VFR25_04500"
     CDS             complement(<1..1829)
                     /locus_tag="VFR25_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940338.1"
                     /translation="MSYAPRDTRSFLLAASVAAMAMIAYQVGAKATRDAFFLSMYPVR
                     YLPAMVAVTSAAALALAYGTTRALSAWGPERLIPTAFLVSGLLMLVEWGVSFVAPGPA
                     SILLYAHYGCLGALLVSGFWQFMNERFDPRSAKRQLGRLTAAATVGGLLGGFVASQIA
                     GVLPLTTMIPILAALHFVCAAAIARVASTSHPIAAVPRSETPAPREEHLNPHAVVART
                     PYIRGLINLVLLATISEGLIDLVLKGRASLAMSEQGSLLRFFAYFYTATSLVTVLVQT
                     SLSRWVLERLGPARSAAALPGGVAIASATAAAIPGLGTTIAARAVESVLANSLFRAGY
                     EVLFTPVPQREKRAIKSLADVGTARAGDIIAAGLAQAVLLIGLEVRQGTVLLFMAAAI
                     SVVGFFIACGLHRGYVSALARGLVSRAVQLDIQDVQDATTRSTVLQTLGPLALSQILQ
                     VPRPDAVEPGSTASLRVEPDGEAAAELARIRDLHSRDVPRVRRTLAGVRIDGTLAIHV
                     IPLLAWDDVASDALSALRRATPDAIGALEESLLDPEVEFTIRRRIPLVLATFQDPRAV
                     QALLGGLRDPRFEVRYRCGRGLAHLAELKSTLTVPPDEAFAAVL"
     gene            complement(1882..2307)
                     /locus_tag="VFR25_04505"
     CDS             complement(1882..2307)
                     /locus_tag="VFR25_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940339.1"
                     /translation="MDDRDLERWAEEVLGVKPEPIPAPTGFTDRVMARVTATSVESRP
                     VPTWLLAASDPVAAVGLTAALVLAMLASWWPERLFGAAALVNAWVWNGTASLARNLDP
                     AALLTLILTSALLIVAVLWRTWRAAERALILALTGRRGS"
     gene            complement(2297..2893)
                     /locus_tag="VFR25_04510"
     CDS             complement(2297..2893)
                     /locus_tag="VFR25_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9940340.1"
                     /translation="MKPEPGTAPSKAGVETSPEVEEIARCRAGDERAYRALVERYKDR
                     AYALAYRMVRSRSDAEEIAQDAFVRAWRGIGEYRGDASFMTWLYRILVRLALDRAETL
                     KSRRAREVLVEEPEEEAPATTADLGLARRMDRLLGALPPAERAALTLFYYEDRPVAEI
                     AQMLDMPENTVKTHMSRGRAALREAYERDQRMGAVRGR"
     gene            2994..3428
                     /locus_tag="VFR25_04515"
     CDS             2994..3428
                     /locus_tag="VFR25_04515"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HET9940341.1"
                     /translation="MHDAYPLISLIIPIIAVAGGITMAIVRIFTQARLEELARKERIA
                     AIERGVDPEKLQPIPASDHYGLGSSRIRRAHGLLIGGLILLAIGLGVSVVFAFVDPEK
                     SNWIAGVVPVFVGIALLISSRVVWPKEAERAGAAGRPGETRY"
     gene            complement(3422..4252)
                     /gene="dapF"
                     /locus_tag="VFR25_04520"
     CDS             complement(3422..4252)
                     /gene="dapF"
                     /locus_tag="VFR25_04520"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET9940342.1"
                     /translation="MLDLDFVKSHGLGNDYVVVDAARFGITLTPERVRLLCHRHLGLG
                     SDGILEVTAADDRFVVRIWNPDGSLAERSGNGLRIVAKFLSDHGYTDESAFSIETVVG
                     PVKTEVYRAEGRVEAVRLEMGKASIDRAVTALPVRSRKLDVTVVSVGNPHCVIFGEPH
                     TRKQLFELGPQVENHAQFPARINVQLAEIESRRRVRALVWERGAGHTLASGTSACAVA
                     AACFDRGLVGSAVTVVMEGGQLEVEVARDLSIIMTGPVEEVFEGTLSADLRRRLAEVQ
                     "
     gene            complement(4254..4904)
                     /locus_tag="VFR25_04525"
     CDS             complement(4254..4904)
                     /locus_tag="VFR25_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9940343.1"
                     /translation="MRDARGSDQALDSGVARLVTLSAAIAAADSESVAREAALALESG
                     VSAAALYEAILQSYLFVGFPRAIEALFAARDVLERAGGIPRAAAPVNLAEWERAGTEL
                     CRRVYGRNYEKLVATMRGLSPDLASWMVLEGYGKTLSRPALGAVEREYAVVAILTVTG
                     AWRQLRSHAIGAVNVGGTRAGVREAITLCAPWAGAAVVEEALGITGLDENGSGQGG"
     gene            4916..6091
                     /locus_tag="VFR25_04530"
     CDS             4916..6091
                     /locus_tag="VFR25_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9940344.1"
                     /translation="MGLGGACQSIGTALAVIAGVTWPVLGLRFALGGRRLASLGKVEP
                     LPDAQLPSLTVIVAAKDEERRVEQAARSLLAQDYPGLTVSVVDDRSTDATPDILGRLA
                     VLDPRLTVTRIDELPSGWTGKSHALAAAAARATTEWLLFTDGDVDLARDACRRAVSLA
                     ARDGRDHLAVAPDLEVETLGEAIFIAYFVIMFHVSQEPWKAQDPRSRGSIGIGAFNLV
                     RRDAYERAGGHAAIRFELIDDLALGRSLKRSGARQAFALPAGLVGVRWHIGVRGLVRG
                     VEKNAFAAVGYRTVPAILAVVAQLTIAVMPALGLFLPGWIPKVAAVAAWAGAALAYFV
                     ASRSVRVRPWQVVFMPVGGILFSYAFLRSTLLTLTRGGVVWRGTFYSLRDLRRRQDR"
     gene            complement(6558..>6910)
                     /locus_tag="VFR25_04535"
     CDS             complement(6558..>6910)
                     /locus_tag="VFR25_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940345.1"
                     /translation="AEDMDPAAVVERARALAPDSKVDQAEGFRWDRLNLYHPSIQALI
                     HTAKARSEGAEIWPSAPWVTSSGLFTHALGTPLAEWTVPLPAGVRVRAPGVEAYRSVV
                     NELSGLFARGVEAK"
BASE COUNT         1072 a         2412 c         2423 g         1003 t
ORIGIN      
        1 agcaccgccg cgaaggcttc gtcgggcggg accgtgagcg tggatttgag ctctgctagg
       61 tgggcgagtc cacggccgca ccgatagcgc acctcgaacc gcggatcgcg gagccctccc
      121 aggagcgcct gcacggcgcg cggatcttgg aacgtcgcga ggacgagcgg gatgcgccgg
      181 cggatcgtga actcgacctc gggatccagg agcgactcct cgagagcgcc gatggcgtcg
      241 ggggtcgcgc ggcgcagcgc cgagagcgcg tcggacgcca cgtcgtccca ggcgagcagc
      301 gggatgacgt ggatcgcgag cgtcccgtcg atccgcaccc cggccagcgt gcggcgcacg
      361 cggggcacgt cgcgcgaatg gagatcgcgg atgcgagcca gctcggccgc ggcctcgccg
      421 tccggctcga cgcggagcga cgccgtcgaa cccggctcca ccgcgtccgg ccgcggcact
      481 tggaggatct ggctcagtgc gaggggaccc agcgtctgga gcaccgtgga gcgggtggtc
      541 gcgtcctgca cgtcttgaat atcgagctgc acggcgcgcg acacgaggcc tcgcgcgagc
      601 gcgctcacgt agccccggtg aagcccgcac gcgatgaaga atccgaccac ggagatcgcc
      661 gcggccatga agaggagcac cgtgccctgc cggacctcga ggccgatgag gaggaccgcc
      721 tgcgcgaggc cggcggcgat gatgtccccc gcccgcgccg tcccgacgtc ggcgagcgac
      781 ttgatcgccc gcttctctcg ctgcgggaca ggggtgaaga gcacctcgta ccccgcgcgg
      841 aagagcgagt tcgcgagcac tgactcgacc gcgcgcgccg cgatggtggt gccgagcccc
      901 gggatcgcgg ctgccgtcgc cgaggcgatc gctacgcctc cgggaagcgc cgccgcggat
      961 cgcgccgggc cgaggcgctc gagcacccag cggctgagcg acgtctgcac cagcactgtc
     1021 acgagcgacg tggccgtgta gaagtaggcg aagaaccgga gcagagaccc ctgctcgctc
     1081 atggccaggg acgcccggcc cttgagcacg agatcgatca gtccctcgct gatcgtcgcg
     1141 agaaggacga ggttgatgag cccgcggatg tagggcgttc tcgccaccac cgcgtgtgga
     1201 ttcaggtgct cctcgcgggg cgccggggtc tccgacctgg gcacggccgc gatggggtgc
     1261 gatgtcgacg cgacgcgcgc gatggcggcg gcgcagacga agtggagcgc cgcgaggatc
     1321 gggatcatgg tggtgagcgg gagcacgccc gcgatctgcg acgccacgaa tccgcccagg
     1381 agtccaccca cggtcgccgc ggcggtgagg cggccgagct ggcgcttcgc ggagcgcgga
     1441 tcgaaccgct cgttcatgaa ctgccagaag ccggatacga ggagcgcgcc caggcagccg
     1501 tagtgcgcgt agagaaggat cgacgcgggc cccggcgcga cgaacgacac gccccactcg
     1561 acgagcatca agagcccgct cacgagaaac gcggtcggga tcaggcgctc cggcccccac
     1621 gcgctgagcg cccgcgtcgt cccatacgcc agggcgagcg ccgcggccga cgtcaccgcc
     1681 accatggccg ggaggtaccg gaccggatac atggacagga agaacgcgtc ccgggtcgcc
     1741 ttggcgccga cctggtaggc gatcatggcc atggccgcga cgctggcggc gaggaggaac
     1801 gaacgggtat cgcgcggggc gtagctcaag gcgtgagaat aacccggcga aggagcgcct
     1861 ggcggaacga cgttggtgag gctacgaccc gcgccggccg gtgagggcga ggatcagggc
     1921 gcgctcggcc gcgcgccagg tgcgccatag caccgcgacg atgagaaggg cgctcgtcag
     1981 gatcagcgtg agaagagcgg cggggtcgag attccgggcg agactcgcgg tcccattcca
     2041 cacccacgcg ttcacgaggg ccgcggcgcc gaagagccgt tccggccacc acgacgccag
     2101 catcgcgagg acgagcgcgg cggtgagccc gaccgcggcg accggatcgc tcgccgcgag
     2161 cagccaggtc gggacggggc gcgactcgac ggaggtcgcg gtgacacggg ccatcacgcg
     2221 gtccgtgaat cccgtcggcg ccgggatcgg ctctggcttc acgccgagca cttcctccgc
     2281 ccagcgctcg agatccctat cgtccacgaa cagctcccat ccgctggtcc cgctcgtacg
     2341 cctcgcgaag cgccgcgcgg ccgcggctca tgtgggtctt caccgtgttc tccggcatgt
     2401 cgagcatctg cgcaatctcc gcgaccggcc gatcttcata gtagaacagc gtgagcgccg
     2461 cgcgctcggc cggcggaagc gcaccgagga gccggtccat ccgccgcgcc aggccgaggt
     2521 cggcggtcgt cgcgggggcc tcttcctcgg gctcctcgac caagacctcg cgcgcccggc
     2581 gcgacttcag ggtctcggcc cggtcgagcg cgagccgtac cagaatgcgg tagagccagg
     2641 tcatgaagga ggcatcgccc cggtactcgc cgatgccgcg ccacgcccgg acgaacgcgt
     2701 cctgcgcgat ctcttcggcg tcggaccggg agcggaccat ccggtacgcg agcgcgtagg
     2761 cgcgatcctt gtagcgttcg acgagcgccc ggtacgcccg ctcgtcgccc gctcggcacc
     2821 gcgcgatctc ctcgacttcg ggggaggtct ccacccccgc cttggacggg gcggtccccg
     2881 gctccggttt catgaaaccc gcccgggggg tcgcgccctc caagtcatcg agtcccggga
     2941 gggcagccca tccaggccac cccggcggat ccttcaaccc aaggagcgaa ggcatgcacg
     3001 acgcgtatcc tctgatctcc ctgatcatcc cgatcatcgc ggttgcaggc ggcatcacca
     3061 tggccatcgt gcgaatcttc acccaggcac gcctcgagga gcttgcccgc aaggagcgta
     3121 tcgcagccat cgagcggggt gtcgatccgg aaaagctcca accgatcccc gcctcggacc
     3181 actacggctt gggaagcagc cggattcgcc gcgcccacgg ccttctcatc gggggactca
     3241 tcctgctggc catcggcctc ggtgtgagcg tggtcttcgc gttcgtcgat ccggagaagt
     3301 cgaactggat cgcgggggtc gtgcccgttt tcgtgggcat cgcgcttctc atctcgagcc
     3361 gggtcgtgtg gccgaaggaa gcagagcggg ccggcgcggc tggccggccg ggcgaaaccc
     3421 gttactgaac ctcggcgagc cgccgcctca agtcggcgct gagcgtgccc tcgaagacct
     3481 cctccaccgg gccggtcatg atgatcgaga ggtcccgcgc gacctcgacc tccagctggc
     3541 ccccctccat caccaccgtc actgcgcttc cgaccaggcc gcggtcgaag caggccgcgg
     3601 ctacggcgca cgcgctcgtg cccgacgcga gcgtgtgccc cgcgcctcgc tcccatacga
     3661 gcgcgcgcac gcggcggcgg gactcgatct cggcgagctg gacgttgatg cgcgcgggga
     3721 actgcgcgtg gttctcgacc tgcgggccga gctcgaagag ctgcttccgg gtgtggggct
     3781 cgccgaagat cacgcagtgg ggatttccga ccgagacgac cgtgacgtcg agcttccggc
     3841 tccgcacggg tagcgccgtc acggcgcggt cgatcgaggc cttgcccatc tcgagccgca
     3901 cggcttccac gcgcccctcg gcgcgataga cctcggtttt caccgggccc acgacggtct
     3961 cgatggagaa cgcggactcg tcggtgtagc cgtgatcgct caggaacttc gccacgatgc
     4021 ggagcccgtt gccgcttcgc tccgcgagcg agccgtcggg attccagatg cgcacgacga
     4081 agcggtcgtc ggccgccgtc acctcgagga tgccgtcgga gccgaggccc aggtggcgat
     4141 ggcagaggag gcgcacgcgc tcgggggtga gcgtgatccc gaaccgcgcg gcgtcgacga
     4201 cgacgtaatc gttgccgagg ccgtgcgact tcacgaagtc gagatcgagc aagtcatcct
     4261 ccctgaccgg aaccattctc atccaggccc gtgatgccga gcgcctcttc caccaccgcg
     4321 gcgcccgccc acggggcgca gagcgtgatc gcctcgcgga ctccggcgcg cgtgccgccg
     4381 acgttcaccg cgccgatcgc gtgcgaccgg agctgccgcc acgccccggt gacggtgagg
     4441 atcgcgacca ccgcgtactc gcgctcgacc gcgccgagcg ccggacgtga gagcgtcttg
     4501 ccgtatccct cgagcaccat ccaggacgcg aggtcgggcg agagcccgcg catcgtggcg
     4561 accagcttct cgtagttccg tccgtagacc cgccggcaga gctctgtgcc tgcgcgctcc
     4621 cattcggcga gattgaccgg cgcggcggcc ctgggaatgc cgcccgcgcg ctcgagcacg
     4681 tcgcgcgccg cgaagagcgc ttcgatcgcg cgtggaaagc cgacgaagag ataggactgg
     4741 agaatggctt cgtagagcgc ggccgccgaa acgcctgact cgagcgccag tgcggcctcg
     4801 cgggcgaccg attcggaatc ggccgccgcg atcgcggccg acagggtgac gagcctggcg
     4861 acgcctgagt cgagcgcttg atcggagcca cgcgcgtcgc gcatcctcgc aatctatggg
     4921 cctgggcggg gcgtgtcaat cgatcgggac cgcgctcgcc gtcatcgccg gcgtgacgtg
     4981 gcccgtcctc gggttgcgct tcgcgctcgg tggccggcgc ctcgccagcc tgggcaaggt
     5041 cgagccgctg ccggacgccc agctcccctc gctcacggtg atcgtcgccg ccaaggacga
     5101 ggagcggcgc gtggagcagg cggcgcgctc gctcctcgcg caggactatc ccgggctcac
     5161 ggtgtcggtc gtcgacgacc gctcgaccga cgccacgccg gacatcctcg gccggctcgc
     5221 cgtcctggat ccgcgcctca cggtgacgcg gatcgacgag ctcccgagcg gatggacggg
     5281 gaagtcgcac gcgctcgccg cggccgccgc gcgcgccacg accgagtggc tcctcttcac
     5341 cgacggcgac gtggatctcg cccgcgacgc gtgccgccgc gcggtctcgc tcgcggcgcg
     5401 cgatggacgg gatcacctcg ccgtcgcccc ggatctcgaa gtcgagaccc tcggcgaggc
     5461 gatcttcatc gcctacttcg tgatcatgtt ccacgtgagc caggagccat ggaaggcgca
     5521 ggatccgcgg tcccggggat cgatcgggat cggcgcgttc aacctcgtcc gccgcgacgc
     5581 ctacgagcgc gcgggcgggc atgcggccat ccggttcgag ctgatcgacg atctcgcgct
     5641 gggacggtcc ctcaagcgaa gcggcgcgcg ccaggcgttc gcgctcccgg cgggactcgt
     5701 cggcgtgcgg tggcacatcg gcgtgcgcgg cctcgtgcgg ggagtcgaga agaacgcgtt
     5761 tgccgccgtc ggctatcgca ccgttcccgc gatcctcgcc gtggtcgcgc agctcacgat
     5821 cgcggtgatg ccggcgctcg ggctcttcct gccagggtgg atccccaagg tcgccgcggt
     5881 cgccgcgtgg gcgggcgcgg ctctcgccta cttcgtcgcg tcgcggagcg tccgagtgcg
     5941 gccgtggcag gtcgtgttca tgccggtggg cgggatcctc ttctcgtacg catttcttcg
     6001 gtcgacgctt ctgacgctca ctcggggcgg ggtcgtctgg cggggcacgt tctattcgct
     6061 gcgtgacctg cggcggcgtc aggacaggta ggtctcaccc gcaaaaccag gcgactgaac
     6121 ccttggacaa caaaagaccc ccgggagcgt ggggctcccg gggggtgtcg tcgcgtatca
     6181 cgtggggggt cagggcattt cgatacgcga cgcaagtgcg tcatcagcgt ctacctgggc
     6241 ttccccgccc cggctccgct gcttgctacg cgatcagcat tggcagaagc gcgatgaaca
     6301 aactcgccgc caccaagccc agctttcgca tcatctcaag gaacctcagg ctaccgttgt
     6361 ccaacggccg cccccctgtc tcatcaatcc ccgcataccg tgtgccatgg atgtcacccc
     6421 tgtcagattt ctcttcaacg caatgcaaca gttcggttaa tggcatcggt aattattaag
     6481 tcacaaggga ttgtcggcgg gttcgcgatc gtgaaggtac agagtcgtac gatcgagtac
     6541 gttgcggagc gtcgagtcta cttggcttcg acacctcgag cgaagagtcc cgacagctcg
     6601 ttgacaacgg atcgatacgc ctcgacaccc ggagcgcgca cacgcacgcc ggctggaagc
     6661 ggcacggtcc actcggccag cggggtgccg agcgcgtgcg tgaacagtcc cgacgacgtg
     6721 acccacggtg ccgagggcca gatctcggcc ccctcggatc gggccttcgc cgtgtggatc
     6781 agggcctgga tcgacgggtg atagagattg agccggtccc atcggaagcc ctcggcctgg
     6841 tcgaccttcg agtcgggggc cagagcgcgg gcacgctcca cgacagcagc cggatccatg
     6901 tcctcggccc
//
LOCUS       DASTKC010000110         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_793252, whole genome shotgun sequence.
ACCESSION   DASTKC010000110 DASTKC010000000
VERSION     DASTKC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793252"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2145)
                     /locus_tag="VFR25_04540"
     CDS             complement(<1..>2145)
                     /locus_tag="VFR25_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9940346.1"
                     /translation="VLPAAALHRLVPQPDRVRARAITLTRGLETDPEDLVRRLVVGGY
                     RAVAEIGESGEVSRRGGIVDVYGPGLERPVRVEFDGDVVASMRAFDITSQRSIGALDR
                     VRIAPAREVLYPEDLDERLRPFDRGSFPSELDGRRLRDLVGEGIYFDGLDWLAPILDV
                     KLTSLLDALPPGCALWLDEPGAIERELETVEREAERLEGEARAKAPHLPPREALFDPP
                     ARVIERIHERTRLESTLAAPAGKADETVAIDARPQPSFGRKLELLRSELKRLGELGYE
                     RIIVCDNRGQAERLEELLGDDFGSIEVGSIGAGFVLPGAKLALFTDHEIFARYRRRGA
                     RRARPSAAVMRDLLTLKPGDYVVHLDHGIGTYRGLKRLELDGQETECVQIDYAGTDRL
                     WVPIHQLGMVQRYSAEEGHAPPIARLGGTAWQRTKAKTRKAIQEMAEELLRLYATRKA
                     RPGHAFSPDTPWQRELESSFVYEETPDQLKAVEDVKRDMESNRPMDRLICGDVGYGKT
                     EVAIRAAFKAIQDGKQVAILVPTTVLAQQHFVTFTERLADFPVRIEMLSRFRSAKEQK
                     EIVARLAKGEVDLVIGTHRILSKDVVFPNLGLVVIDEEQRFGVKQKERIRSLVETVDV
                     LTLTATPIPRTLHMSLLGARDMSIMTTPPRGRYPIKTEIVEMSQDVVRDALLREADRG
                     GQSFFVHNRVESIDRMAHYLTSIVPQLRYGVA"
BASE COUNT          315 a          731 c          756 g          343 t
ORIGIN      
        1 tgcgcgacgc cgtagcggag ctggggcacg atcgaggtca gatagtgcgc catccggtcg
       61 atcgactcga cccggttgtg cacgaagaag gactgccccc cgcgatcggc ctcgcggagg
      121 agagcgtccc gcaccacgtc ctggctcatc tcgacgatct cggtcttgat cggatagcgg
      181 ccgcgcggcg gcgtggtcat gatcgacatg tcgcgcgcgc cgagaaggct catgtggagc
      241 gtgcgcggga tcggcgtggc cgtgagggtg agcacgtcga ccgtctccac gagcgagcgg
      301 atgcgctcct tttgcttcac gccgaagcgc tgctcctcgt cgatcaccac gagcccgaga
      361 ttcgggaaga cgacatcctt cgagaggatc cggtgcgtgc cgatcacgag atcgacctcg
      421 cccttggcga gccgcgccac gatctccttc tgctccttgg ccgagcggaa gcggctcagc
      481 atctcgatcc ggaccggaaa gtccgcgagc cgctcggtga acgtgacgaa gtgctgctgc
      541 gcgagaaccg tcgtggggac caggatcgcg acctgcttcc cgtcctggat cgccttgaac
      601 gcggctcgga tcgcgacctc ggtcttgccg tagccgacgt cgccgcagat gaggcggtcc
      661 atgggccggt tggattccat gtcgcgcttc acgtcctcca cggccttcag ctgatcgggc
      721 gtctcctcgt agacgaagga cgattcgagc tcgcgctgcc acggcgtatc gggcgagaag
      781 gcgtgcccgg ggcgggcttt ccgcgtcgcg tagaggcgga ggagctcctc cgccatctcc
      841 tggatcgcct tccgcgtctt cgccttcgtg cgctgccacg cggtgccgcc gaggcgcgcg
      901 atcgggggcg cgtgtccttc ctcggcggaa tagcgctgca ccatgccgag ctggtggatg
      961 ggcacccaga gccggtcggt gccggcgtag tcgatctgga cgcactccgt ctcctgcccg
     1021 tccagctcga ggcgcttcaa cccgcgatac gtgccgatgc cgtggtccag atggacgacg
     1081 tagtcgcccg gcttgagcgt gaggagatcg cgcatgaccg cggccgacgg acgcgcgcgg
     1141 cgcgcgccgc gccgccggta gcgcgcgaag atctcgtgat cggtgaagag agcgagcttc
     1201 gccccgggga gcacgaagcc ggcgccgatc gagcccacct cgatcgaccc gaagtcgtct
     1261 ccgagcagct cctccagccg ctccgcctgc ccgcggttgt cgcagacgat gatccgctcg
     1321 tagccgagct cgccgaggcg tttcagctcg ctgcggagga gttcgagctt ccgtccaaac
     1381 gagggctgcg gcctcgcgtc gatcgcgacg gtctcgtccg ctttccccgc gggagccgcg
     1441 agcgtggact cgagccttgt cctctcatga atgcgctcga tcacgcgcgc cgggggatcg
     1501 aagagcgcct cgcgcggggg caggtgcggc gccttggcgc gcgcctcgcc ttcgagccgc
     1561 tcggcttcgc gttccaccgt ttccagctcg cgctcgatgg cgcccggctc gtcgagccac
     1621 agcgcgcatc cgggcggaag cgcgtcgagg agcgaggtga gcttcacgtc caggatcggc
     1681 gcgagccagt cgagtccgtc gaagtagatg ccttcgccca cgagatcgcg gagccggcgt
     1741 ccgtcgagct ccgacgggaa ggacccccgg tcgaacggac ggagccgctc gtcgaggtcc
     1801 tcgggataga ggacttcacg ggcgggggcg atacggacgc gatcgagcgc tccgatggaa
     1861 cgctgactcg tgatgtcgaa ggcgcgcatg gacgccacca catcgccgtc gaactcgacg
     1921 cgcaccgggc gctcgagccc ggggccgtat acgtcgacga tgccgccgcg ccggctcacc
     1981 tcgcccgatt cgccgatctc ggccacggcc cgatatccgc cgaccacgag acgccggacg
     2041 agatcctcgg ggtcggtttc gaggccgcgc gtgagcgtga tggcgcgggc gcgcacgcgg
     2101 tccggttggg gcacgaggcg gtggagcgcg gccgctggga gcacc
//
LOCUS       DASTKC010000111         7517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_320414, whole genome shotgun sequence.
ACCESSION   DASTKC010000111 DASTKC010000000
VERSION     DASTKC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320414"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VFR25_04545"
     CDS             <1..1434
                     /locus_tag="VFR25_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9940347.1"
                     /translation="NDNFNTPLGDSAVVQVSTSRGYKTGFMFYRFGTSGSWLSTPLLM
                     SDPAAPTFRYADPPPANYPAGTVLQYYFAVTDSVDSTAYLPAKAPQTATYLSASILPV
                     KSATNPVLGCTDSLATVLFVNNNSGREAEATIAKVLTAQGYKYDTWDVNGPTSGAGNT
                     PGGTAPGDPFNIWPGSTVNDLLRYSAIIWHAGSLSQFTIPQTDQQLIQSWVQQPGKSR
                     NFFLAGDNVAQELITLGAEYNAFLSFTVGARWLRSLWENTPQDTLHPLLTGFAGSPSA
                     GRLMHLNLDCPQIEDPDLIAVATTAPSRGKTGLWLKYPNTFAAGTRFATKYVSFGTDS
                     SRNLFFAFNYNNIEESGERLRAIKDIMLTYFQIPACYFATAVEEDQSPAPTGVDVLYQ
                     NSPNPFNPETVIRYSVAQSGPVTLRIFNVQGALVRTLVNKQHVAGSYRVRWDGTNDYG
                     EHLASGVYFYRLETRNAADSKKLIMLK"
     gene            1683..2255
                     /locus_tag="VFR25_04550"
     CDS             1683..2255
                     /locus_tag="VFR25_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9940348.1"
                     /translation="MNGQLRIRSRRFATLLLGPALTAALLVAPMGRALAGEIIPSVGL
                     TRSVDGDEAKSLVGLALRGSLIPGVVKTEVGVQYRQESMYNGDLKLRQWPVTASLWLA
                     PVPALYAGAGVGWYQTTFDYANDTGINDTTHQDFGVHVGGGVRVPIVPHANLDLNGRY
                     VFMQDQESHLVPEKFNPDFWSMTAGVAIGF"
     gene            2278..2790
                     /locus_tag="VFR25_04555"
     CDS             2278..2790
                     /locus_tag="VFR25_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940349.1"
                     /translation="MNPNSKKFLAMATALMMGAILMALAIPHPANAFHLVGVGPRVGF
                     TDPDGVDNTVNVGGGLDFEQGGSRIHLVPSLLYWNNEGLSDVNPNFDAMYHFSPAGRV
                     SPYLGAGLGIHAYSQDGPGDPGTDVGANLFGGLLFPSRGTSFFVEGRAVIADRDQIGM
                     LGGVNFNLGR"
     gene            3020..5293
                     /locus_tag="VFR25_04560"
     CDS             3020..5293
                     /locus_tag="VFR25_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9940350.1"
                     /translation="MLRTDAEPDSARPPSSPSAARWGEFSLLERLGAGTFGEVWRAWD
                     PALRREIAVKFLRERSAATGGVHQAALLDEARALARIRHPGVAAVYGVAEFDGRAGLW
                     MEYLRGPSLASVLEERGAMPWRDVIRVGCELCSALEALQHAGLVHRDIKPANVVMESD
                     GRTVLADFGLGMRRRLLDPNEMKQGWGTPLFMAPSLLRGGTPTFRTDLYAVGVTLLWA
                     LIGRAPFESRTLPDLQIESARGPTIALPDSNRDAPVALLDLIGKSLEESGAARFTASK
                     MRAGLESLQGANQARDLAARPSIAVLPFLNRSADESTDYFSDGLADELVMVLSKIQGL
                     RVIARTASFQFKGKTEDLAVIGAKLNVATLLEGSVVKQDRRLRVSVRLVKIPDGDTLW
                     SESYDRTWDDVFAVQDDIAQRVVTELRARLLGAALDPARAKAEIAEAVRGRGEDPEVH
                     RLLMQARYHLDRVTSDDAAQALEVVAQAMERDAANPALWCELCRARSLQMNFGWIPIA
                     EGTVLAREAIDRALELDPELPEAHGLLGWLLMHYDRNWAAAEASFLRALDLDPESASN
                     LRRAGTLAMCLNRPLQAIDYYDRSLIGDPLSTAAYNNLGFSHYIAGHYEEAVRAIQKS
                     IELAPQRVGARALLAFTLQEMGRGEEAMAAANEETMEVYRVWGLAIIHHYEGRHDESN
                     QMLALLEEKYADEAAYQIAEVHAARGAVDMAFEWLERAYRQHDGGLAGMKASVNFRPI
                     HSDPRWNTFLLKVGFDA"
     gene            complement(5320..6708)
                     /gene="lpdA"
                     /locus_tag="VFR25_04565"
     CDS             complement(5320..6708)
                     /gene="lpdA"
                     /locus_tag="VFR25_04565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9940351.1"
                     /translation="MADASHDLVVIGSGPGGYVAAIRAAQLGLDVGCVELEEALGGTC
                     LRIGCIPSKAMLESSERYHEAKAALAPHGVKVSQVELDLPTMLKRKDEVVRGRTQGVD
                     FLFKKNKVTRYKGVGTIDGSGRVRVKSGPQTTALAARNILIATGSKPASLPGVEWSGD
                     KIGSSTEALAYASVPKHLVVIGAGYIGLELGSVWLRLGAKVTVVEYLDRILPGMDSEI
                     AREGQKLLERQGMEIRLKQKVTAAKVKGKDCVVECEGQEPIKADRVLVAVGRAPNTEG
                     LGLASVGIQVDERGRVPVDAHYQAAEGVYAIGDVIGGAMLAHKAEEEGIACVEGIVTG
                     YGHVNYDAIPGVVYTQPEIATVGRSEDELKADGAAYRKGVFFFRANARAHALGQIDGR
                     VKVLADASTDRILGVHIIGPRAGDMIAEAVAAMEFGATSEDVARTCHAHPTLAEALKE
                     AALAVDGRAIHS"
     gene            complement(6710..>7517)
                     /gene="sucB"
                     /locus_tag="VFR25_04570"
     CDS             complement(6710..>7517)
                     /gene="sucB"
                     /locus_tag="VFR25_04570"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET9940352.1"
                     /translation="EDVLDQMTRKPAPSPAAPSKPAAPVPAWTPPPDTGERIEEAVPM
                     SPMRLRIAQRLKEAQNTSALLTTFNEIDMTQVIAVREELREAFQERFQVKLGFMSFFV
                     KASIDALMTFPQVNAEIRDKDVLYRNYYDIGIAVGGGKGLVVPVLRGAERLSFAEIEL
                     KIADFGARARDNKLHLDELKGGTFTVSNGGVYGSLLSTPIINPPQSGILGLHAIQKRP
                     VVVDDQIVIRSMMYVALTYDHRIVDGRESVAFLKHIKELVERPTRMLLGI"
BASE COUNT         1222 a         2538 c         2456 g         1301 t
ORIGIN      
        1 aacgacaact tcaacacccc gctcggcgac agcgcggtcg tgcaggtgag cacgtcgcgc
       61 ggttacaaga ccgggttcat gttctaccga ttcggcacgt ccggatcgtg gctctccacg
      121 cccctcctca tgagcgaccc ggcggcgccc acgttccggt atgccgaccc gccgccggcg
      181 aactatcccg ccggcacggt gctccagtac tacttcgcgg tcacggacag cgtggattcc
      241 accgcgtatc ttccggcgaa ggcgccgcag acggcgacgt atctctccgc ctcgatcctg
      301 ccggtgaagt ccgccacgaa tccggtcctg gggtgtacgg acagcctcgc gaccgtcctc
      361 ttcgtgaaca acaactcggg acgtgaggcc gaggcgacca tcgcgaaggt gctcacggct
      421 caggggtaca agtacgacac ctgggacgtg aacgggccca cctcgggggc ggggaacacg
      481 ccgggaggca cggcgccagg cgatcccttc aatatctggc ccgggtccac ggtcaacgac
      541 ctccttcgct attcggccat catctggcac gcgggaagtc tgagccagtt cacgattccg
      601 cagacggatc agcagctgat ccagtcctgg gtccagcagc ccggcaagag ccgcaacttc
      661 ttcctggcgg gagacaacgt ggcgcaggag ctgatcacgc tcggcgctga gtacaacgcc
      721 ttcctcagct tcacggtggg cgcacggtgg cttcgcagcc tctgggagaa caccccgcag
      781 gacacgctcc acccgctctt gaccggcttc gcgggctcgc cctcggcggg ccggctcatg
      841 cacttgaacc tggactgccc gcagatcgag gacccggatc tcatcgccgt cgccacgacg
      901 gctccgagcc gtgggaagac cgggctctgg ctcaaatatc ccaacacgtt cgcggcggga
      961 acgcgcttcg ccacgaagta cgtgtccttc ggcacggaca gctctcgaaa cctcttcttt
     1021 gcgttcaact acaacaacat cgaggaatcc ggcgagcgtc tgcgtgccat caaggacatc
     1081 atgctcacgt acttccagat cccggcctgc tatttcgcga ccgcggtcga ggaggatcaa
     1141 tcccccgcgc cgaccggagt cgacgtcctc tatcagaact ctccgaaccc gttcaatccg
     1201 gagacggtca tccgctattc cgtggcgcaa tccggacccg tcacgctgcg aatcttcaac
     1261 gtgcagggcg cgctcgtccg gacgctcgtc aacaagcagc acgtggcggg gagctatcgc
     1321 gtgcggtggg acggcacgaa cgactacggc gagcatctcg cgagcggcgt ctacttctat
     1381 aggctcgaga cgaggaacgc agcggattcc aagaagctca tcatgctgaa gtagggcatc
     1441 gcgcgcccct gcgcgccgtt tcgaaacact cccctcaggg gacccccgaa agaacaaatc
     1501 acaatcccgg aaggaccgga aactacatcc ccgggacccc catccagcct gcctgaccca
     1561 gtttttaagt cgttgcgccc cagcgactaa cgcgctcaaa tctgccctca tccgctccca
     1621 tggcacgccg cttgatggtg tcccctggtg aatcccggaa tcatccggag gaggaggtct
     1681 cgatgaacgg acaactacgc atacgcagca ggcgcttcgc gaccctgctt ctgggaccgg
     1741 ccctcacggc cgcgctcctc gtggcaccga tgggtcgcgc tctggccggc gagatcattc
     1801 cgtccgtcgg gctcacacgc tctgtcgacg gcgatgaagc caaatccttg gtcgggctcg
     1861 cccttcgcgg ctcgctcatt cccggcgtgg tgaagacgga agtcggcgtc cagtaccgcc
     1921 aggagtcgat gtacaacggt gatctcaagt tgcggcagtg gcccgtgacg gcttctctgt
     1981 ggctcgctcc ggtcccggcc ctgtacgcgg gcgccggcgt gggctggtac cagaccacgt
     2041 tcgattacgc gaacgacacg ggcatcaacg acaccacgca tcaggacttc ggagtgcacg
     2101 ttggaggagg tgtgcgcgtt ccaatcgtgc cacacgcgaa tctcgacctg aacggccgct
     2161 acgtcttcat gcaggaccag gagtcgcatc tggttcccga gaaattcaat cccgacttct
     2221 ggagcatgac ggccggtgtg gcgatcggct tctgatgagg gaggacctat gaatcccatg
     2281 aatcctaatt cgaaaaagtt tctcgcgatg gcgacggcgc tcatgatggg agcgatcctc
     2341 atggcgcttg cgatcccgca ccccgcgaac gcgttccatc tcgtcggtgt cggcccgcgg
     2401 gtcggcttca cggatcccga cggcgtggac aacacggtga acgtgggcgg cggtttggac
     2461 ttcgagcagg gcggaagccg gatccatctc gttccgagct tgctctactg gaacaacgaa
     2521 ggcttgagcg acgtcaatcc gaacttcgac gccatgtacc acttcagtcc cgccggtcgt
     2581 gtgagcccct atctgggcgc cggcctcggg atccatgcct attcacagga cggtccgggt
     2641 gatcccggaa cggacgtggg agcgaatctc ttcggtggtc ttctcttccc gtcccgcggc
     2701 accagcttct tcgtggaagg ccgcgccgtc atcgccgacc gtgaccagat tggcatgctc
     2761 ggcggagtga acttcaacct cggtcggtaa gcctcgcgag cgcacgtttc cccgcgcacg
     2821 ccgctccggc cagtatgggc ccgcccctca tcggggcggg ccccctcgct ttttgggcag
     2881 acggcgcgta agcttcacgg acgtcaggcc aacctcgaag cgaactcctc cccgcctgga
     2941 gacggccgtc agcgattccc cggtcccgcc ggaaccgctt cgcgattccc gttcgaggac
     3001 tgatcccgca cccaccgaga tgctccgcac ggacgccgag ccggattccg cgcgccctcc
     3061 gtcctctccg tccgccgcgc gctggggcga attctcgctg ctcgagcgcc tcggagccgg
     3121 aacgttcggg gaagtgtgga gggcttggga tccggccctg cggcgcgaga tcgccgtgaa
     3181 attcctccgc gagcggtccg ccgcgactgg cggagtgcac caggcggctc tcctcgacga
     3241 ggcgcgcgcc ctcgcgcgga tacggcatcc cggagtggcg gcggtgtacg gcgtcgcgga
     3301 gttcgacgga agagccgggc tgtggatgga gtacctgcga gggccgagtc tcgcctccgt
     3361 gctcgaggag agaggggcga tgccgtggcg cgacgtgatt cgcgtgggct gcgagctgtg
     3421 ctccgcactc gaggcccttc agcacgcagg tctggtccac cgggacatca agcccgccaa
     3481 cgtcgtgatg gaatccgacg ggcggacggt gctcgccgac ttcggactgg gcatgcgtcg
     3541 ccggctcctc gatccgaacg agatgaagca gggctgggga acgccgctct tcatggcgcc
     3601 gagcctgctt cgtggcggca cgcccacgtt ccggacggac ctctatgccg tgggtgtgac
     3661 gcttttgtgg gcgctcatcg gtcgtgcccc gttcgagtcg cgcaccctcc cggatctcca
     3721 gatcgaatcg gcgcggggac cgaccatcgc gcttcccgac tcgaaccggg acgcgcccgt
     3781 ggcgctcctc gacctgatcg ggaagagtct cgaggagtcg ggagccgcgc gattcacggc
     3841 atcgaagatg cgtgccggcc tcgagtcgct tcagggcgcc aaccaagcgc gggatctcgc
     3901 cgcccggccc tcgatcgccg tcctgccgtt cctgaaccgg agcgcggacg aatcgaccga
     3961 ctacttctcg gacggactcg ccgacgagct ggtgatggtt ctctccaaga tccaggggct
     4021 ccgcgtcatc gctcggaccg cctcgttcca gttcaagggc aagaccgagg atctcgcggt
     4081 gatcggtgcc aagctgaacg tcgcgaccct gctcgaggga agcgtcgtca aacaggaccg
     4141 ccggctccgc gtgtcggttc gtctggtcaa gatcccggat ggggacacgc tctggtcgga
     4201 gagctacgac cggacgtggg acgacgtctt cgcggtccag gacgacatcg cgcagcgcgt
     4261 cgtcaccgag ctgcgcgcac ggctcctggg cgccgccctg gatccagccc gagccaaggc
     4321 ggagattgcc gaggcggtcc gcggccgcgg cgaggatccc gaagtccatc gccttctgat
     4381 gcaggcgagg taccacctcg atcgcgtgac atccgacgac gcggcccagg cgctcgaggt
     4441 cgtggcgcag gcgatggagc gcgatgctgc caatccggcg ttgtggtgcg agctgtgccg
     4501 ggcgcgcagc ctgcagatga acttcggatg gatccccatc gccgagggaa ccgtcctggc
     4561 gcgcgaggcc atcgaccgcg cgctcgagct ggatccggag ctgcccgaag cgcacgggct
     4621 cctgggctgg ctgctcatgc actacgaccg gaactgggcc gcggccgagg cctcctttct
     4681 gagggcgctc gatctcgatc cggaatcggc cagcaatctc cgcagagccg ggactctggc
     4741 catgtgcctc aatcgcccgc ttcaggcgat cgattactac gaccgctcct tgatcgggga
     4801 tccgttgagc accgcggcgt acaacaatct cggcttctcc cactacatcg ccggccatta
     4861 cgaggaggcg gtgcgcgcga ttcagaagtc catcgagctg gcgcctcagc gtgtgggggc
     4921 gagggcgctc ctggcattca cgctccagga gatggggcgt ggtgaggagg ccatggcagc
     4981 ggcgaatgag gagaccatgg aggtctaccg agtttggggc ctcgccatca tccaccatta
     5041 tgagggccgg cacgacgagt cgaaccagat gctcgcgctg ctcgaggaga agtacgccga
     5101 cgaggccgcc taccagatcg ccgaggtcca cgccgcccgg ggcgcggtgg atatggcatt
     5161 cgagtggctc gagcgcgcgt accggcagca cgacggcggg ctcgccggca tgaaagcgag
     5221 cgtcaacttc cgcccgatcc actccgaccc gagatggaat acgttcctgc tcaaggtggg
     5281 gttcgacgcc taggcgcgcg gggcgcgttc cgggcgcggc tagctgtgga tcgcgcggcc
     5341 gtcgacggcc agcgcggctt ccttgagagc ttccgccaag gtgggatggg cgtggcaggt
     5401 gcgggccacg tcttcggagg ttgcgccgaa ctccatcgcg gcgacggcct cggcgatcat
     5461 gtcgccggcc cggggcccga tgatgtggac gccgaggatg cggtcggtgg aggcgtcggc
     5521 gagcaccttc acccggccgt cgatctggcc gagcgcatgg gcgcgagcgt tcgcgcggaa
     5581 gaagaagacc cctttccggt acgcagcgcc gtcggccttc agctcgtcct cggagcggcc
     5641 gaccgtcgcg atctcgggct gcgtgtagac caccccgggt atcgcgtcgt agttgacgtg
     5701 tccgtagcct gtgacgatcc cctcgacaca ggctattcct tcctcttccg ccttgtgcgc
     5761 gagcatggct ccgccgatca cgtcgccgat ggcgtacaca ccttcggcag cctggtagtg
     5821 ggcgtcgacc gggacccgcc cgcgctcgtc gacttgaatg ccgaccgacg cgaggccgag
     5881 cccctccgtg ttgggcgcgc ggcccacggc gacgaggacg cggtcagcct tgatcggctc
     5941 ctggccctcg cactcgacca cgcagtcctt ccccttcacc ttcgccgctg tgaccttctg
     6001 cttgagacgg atctccatgc cctggcgctc gaggagcttc tgtccctcgc gcgcgatctc
     6061 cgagtccatt ccgggaagga tgcggtcgag gtattcgacc accgtgacct tggctcccag
     6121 gcggagccag acggagccaa gctcgagtcc gatgtagccg gcgccgatca ccacgagatg
     6181 cttgggcacc gacgcgtacg cgagcgcctc cgtgctcgag ccgatcttgt cgccgctcca
     6241 ctcgacgccg ggcagggagg cgggcttgct tcccgtggcg atgaggatgt tcctggccgc
     6301 gagggccgtc gtctggggtc cgctcttcac gcgaacgcgc cccgatccgt cgatcgtgcc
     6361 cacgcccttg tagcgggtga ccttgttctt cttgaagaga aagtccacgc cctgcgtccg
     6421 gccacggacg acctcgtcct tgcgcttgag catggtcggg agatccagct cgacctggct
     6481 caccttcacg ccgtgcggcg cgagcgcagc cttcgcctcg tggtagcgct cgctcgactc
     6541 gagcatggcc ttgctcggga tgcagccgat ccggaggcag gtcccgccga gggcttcctc
     6601 gagctcgacg cagccgacgt cgagcccgag ctgcgcggcg cggatggcgg cgacgtagcc
     6661 tcccggtcca gagccgatca cgaccaggtc gtgcgatgcg tcggccatct cagatcccga
     6721 gcagcatgcg ggtgggccgc tcgaccagct ccttgatgtg cttcaggaag gcgaccgatt
     6781 cgcggccgtc gacgatgcga tggtcgtacg tgagcgccac gtacatcatg gagcggatca
     6841 cgatctgatc gtccacgacg acggggcgct tctggatggc gtggagcccg aggatgccgc
     6901 tctgcggcgg gttgatgatc ggggtcgaga ggagcgagcc gtacacgccg ccgttcgaca
     6961 cggtgaacgt gccccccttg agctcgtcca ggtggagctt gttgtcccgc gcgcgcgcgc
     7021 cgaagtccgc gatcttgagc tcgatctcgg cgaagctcag ccgctccgcg ccgcggagca
     7081 cgggcacgac gagccccttc ccgccgccga cggcgatccc gatgtcgtag tagttccggt
     7141 agaggacatc cttgtcccgg atctcggcgt tcacttgtgg gaacgtcatg agggcgtcga
     7201 tggatgcctt cacgaagaag gacatgaacc cgagcttgac ttggaagcgc tcctggaacg
     7261 cctcgcgcag ctcctcgcgc acggcgatca cctgggtcat gtcgatctcg ttgaacgtcg
     7321 tcaggagcgc cgaggtgttc tgcgcctcct tgaggcgctg cgcgatccgg agccgcatcg
     7381 ggctcatcgg caccgcctct tcgatccgtt cgcccgtgtc ggggggcggc gtccatgcgg
     7441 ggaccggagc ggcgggcttg gacggcgccg cgggggacgg cgcgggcttc cgcgtcatct
     7501 gatcgaggac gtcctcc
//
LOCUS       DASTKC010000112        17256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_84294, whole genome shotgun sequence.
ACCESSION   DASTKC010000112 DASTKC010000000
VERSION     DASTKC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17256
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84294"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1085)
                     /locus_tag="VFR25_04575"
     CDS             complement(105..1085)
                     /locus_tag="VFR25_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9940353.1"
                     /translation="MTPGGSDLEPRRIELYRPQNQLGDLLLNVPAIRALRDRYPGAHI
                     TLVVGPQNAPAVLGQAWVDRVRVVPTKGVGALVGALTRSGPRADLAIYFTTVSYSKSA
                     AWLVRSSGATRRIGFEAARYGERDRAALTEMVPYPDGEPHQSEVSLALARAAGAGAAV
                     PPPPSYVPDAALLERAPGGHVYLHPGAGKVKNRWPAERFAEVARALLARGCEISWIEG
                     PQDEGCVDRASSALGRTLPVVRGEAIPMLAARFARAALYIGNDTGPLHLAAATGCATV
                     GIYGWSDPAQWAPVGRCVRSVRAADATLESIPPEQVLEVALPLLEETACV"
     gene            complement(1082..1333)
                     /locus_tag="VFR25_04580"
     CDS             complement(1082..1333)
                     /locus_tag="VFR25_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9940354.1"
                     /translation="MMTCKEVLESLSGYLDEDLKQEVAVEMKKHIGLCTDCRAEFDTL
                     TLTIKLYRHFETPSMPADCHDRLVKVLELEKLKRTPGKE"
     gene            complement(1333..1932)
                     /locus_tag="VFR25_04585"
     CDS             complement(1333..1932)
                     /locus_tag="VFR25_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9940355.1"
                     /translation="MTEAETDEQLVRKSQQDNERAFGELVSRYESKVYSLALKMLRNP
                     EDAEDVLQDTFLRAYRGIKSFKGNSTFSTWIYRITANSALMRLRKRQLPTVSIDDADE
                     REAPINIADWAPGPVEQMLNQETQAAMTEAIDALPPEFRQVFVLRDIEELSNAEVAEI
                     LDLSVAAVKSRLHRARLKVRNRLATYFTEPRTRRAMGAQ"
     gene            complement(2041..3459)
                     /locus_tag="VFR25_04590"
     CDS             complement(2041..3459)
                     /locus_tag="VFR25_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9940356.1"
                     /translation="MNLNVGIIMPAGSERERLVQHVEDSGCPVLVTEHVAEFLAHAQA
                     DSCGLALLDFGDSENGGPDHAERRDAIKRVKERYPRLQLALWLDRSSAAEAAEMNELG
                     IRNLYLKPVRFDPIDGLLKAAGKSAAQQARQQRETARVRDEFRFDRIVGQSAALKDSI
                     GLAQSVAESSATSVLLLGESGVGKELFARAIHGESPRSHGPFLEINCAAIPRELLESE
                     LFGHEKGAFTDATQQRVGLFEAAEHGSVFLDEIGELPLTLQAKLLKFLDSKIVRRVGG
                     NRDIPVDVRILAATNRSLVDEVRQGRFREDLYYRLSVVPISIPPLRDRGDDAALLARL
                     FVERVGRKLGKKAHLSSAGEKDIARYGWPGNVRELMNAIERAVLLARTEEIGPDELAI
                     PKPRDASGQEIGLSDLNLRIPSEGVSLVAVEKAVILGALARTHGNVVEAARLLHIGRG
                     SLRCKMRRHGLGREEPVRVGAV"
     gene            complement(3600..5858)
                     /locus_tag="VFR25_04595"
     CDS             complement(3600..5858)
                     /locus_tag="VFR25_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9940357.1"
                     /translation="MLGKTLTHYTLIDRLGGGGMGVVYRARDLRLNRDVAVKVLAPAI
                     LTNESARLRFRREAMALSRLSHPNIATVHDFDCADGIDFLVMEHVEGETLAQRLLRGS
                     LSEADATPIAIQIAEALEEAHEQGVVHRDLKPANVAITPKGRVKVLDFGLARLLQTSD
                     AADTMALTDAGSWSGTLPYMAPEQLEGKDVDGRADLYALGALLYEMLAGRRPFTAMGA
                     GPLMKAILTERPQPLTRLRPVPPRLEALVARLLEKEADRRPRTARDVITELKGDGTSA
                     TPTSTAPSIRGLVVLPLENLSGDPEQEFFADGMTEELIADLAQIQALRVISRTSAMRY
                     KKTTKTLQEIGRELNVEAIVEGSVRRHADRVRITAQLIEVATDRHLWAKSYERDLKDV
                     LALQGEVARAIAQEIRVKVTPEEEARLARTHVVDPRAYESYLRGRQHWNKRNDEGLLR
                     SLEYFREAIDLDPEWATARAGLADAYCVIGFYGTLPPGDSFPKAKIAANAALRMDDRL
                     AEAYSALGYVQHYYEWDWAAAERSFRRALELNEGSAYIHLFYANMLIAMGRYEESIRT
                     VTRGHELDPLSMIIRAARAWVHFFGGHVDDAVRFIEELMDREPDFSTAFLWASPIYEV
                     RGEYEKALRVAIRGGEMTARNPWALSGMGRALAKMGRRTEAEAILNELRDLASRRYVS
                     PFDLALVCEALGLDDEAFAQLDRGFEVRANMMVLLGVDPRWDRLRPDPRFAELVLRMR
                     FPATVKAPQPAR"
     gene            complement(5960..6457)
                     /gene="msrA"
                     /locus_tag="VFR25_04600"
     CDS             complement(5960..6457)
                     /gene="msrA"
                     /locus_tag="VFR25_04600"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9940358.1"
                     /translation="MTSREPAKATFAAGCFWGVESMFRAIPGVLDAVVGYTGGRTVNP
                     SYEQVCTGRTGHAEAVEVTYDPERVSYETLVQVFFENHDPTTPNRQGPDVGEQYRSAI
                     FTHSPEQDRQAHEAVDKLTEAKRFRRSIVTQILPAAPFYPAEEYHQRYFEKRGIAPTC
                     HVMPK"
     gene            complement(6613..8526)
                     /gene="dnaK"
                     /locus_tag="VFR25_04605"
     CDS             complement(6613..8526)
                     /gene="dnaK"
                     /locus_tag="VFR25_04605"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9940359.1"
                     /translation="MGKTIGIDLGTTNSCVAVMEGGEPVVISNSEGTRTTPSVVAFTK
                     TGERLTGQVAKRQAVTNPKNTIYSIKRFMGRRYAEVAEEAKNVPYAVKDGKNGEAVID
                     VEGKQYSPPEISAMILQKMKQTAEDYLGEKVTEAVITVPAYFNDTQRQATKDAGKIAG
                     LDVKRIINEPTAAALAYGLDKKKDEKIAVYDLGGGTFDISILELGDGVFEVKSTNGDT
                     HLGGDDFDKRVIDWLADEFKKQEGIDLRKDAMALQRLKEAAEKAKIELSTVMQTDVNL
                     PFITADASGPKHLNLTLTRSKFEQLVDDLIERTKGPVERALADAGLKASDIDEVILVG
                     GATRTPKVQELVKKLFGGKEPHKGVNPDEVVAIGAAIQGGVLAGDVKDVLLLDVTPLS
                     LGIETLGGVTTRLIERNTTIPTKKGETFSTASDNQTQVEIHVLQGERQMAQDNKTIGR
                     FTLTGLPPAPRGLPQIEVTFDIDANGILHVAARDKATGKEQKIKIEASGGLSDTEIKR
                     MVSDAESHADEDKKRREDIEVKNRGDALAYETEKNLKELGDKVDAESRSKVESALETL
                     KKAVERNDTTEIRSASEALTQVWNEVSSKLYAEASKGQGGQEPPQGEPQGAPQDKREG
                     GDGAVDADYEVVK"
     gene            complement(8616..8987)
                     /locus_tag="VFR25_04610"
     CDS             complement(8616..8987)
                     /locus_tag="VFR25_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9940360.1"
                     /translation="MSRSQGSPVAWQPAVDVEEHPEGFTIRTELPGMKLEDVKITFAD
                     NQLVIRGEKRREEEKKEANYIRTERVYGQFERAFTLSHAVQPERISATYRDGVLEVNV
                     PKAEEAKSREIPIKTSQGTQS"
     gene            complement(9302..10372)
                     /locus_tag="VFR25_04615"
     CDS             complement(9302..10372)
                     /locus_tag="VFR25_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9940361.1"
                     /translation="MEEASRAARTADSAAELEARDPASRGLWEATVQTDEERKPSGIT
                     QEIVGVSPRMQRVFRLVEKVAPTESTVLITGESGTGKEMVAASIHLQSARAHKPFVTV
                     NCAAIPETLFESELFGHVRGSFTGAISDKVGLLKRADGGTIFLDEVAEMPLSVQPKLL
                     RALQNGEIRRVGDAESSRIDVRVIAATNRDVKRALAEGRLREDLYYRLSVFHIDLPPL
                     RDRREDIPLLANYFRQRFARRLKKRVERFSERAQYALLHYDYPGNVRELENAIERAVT
                     LAEGPEIGHMDLPPSFREPSVLVLGEGNAFPYSETMSLAQIEQEHIRRALSHFAGNTT
                     RTARSLGISRSTLWRKMKQYRI"
     gene            10510..11826
                     /locus_tag="VFR25_04620"
     CDS             10510..11826
                     /locus_tag="VFR25_04620"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET9940362.1"
                     /translation="MILFFISGIVLVAFAAVLPRLGDLGSKAHLLLYLWAIAHAAYLL
                     AAYRALRAPAGAASKRALGWILGMALLARLLLIPAAPTLSEDAYRYLWDGSLTRDGIN
                     PFVHAPSDPALAERRNDLFARLNHADVPTIYPPAAQLFFGAVASVRATPAALKAAYLP
                     IEIAAWIALLALLRRRGLAPERILLFAWNPLVLVESYGSGHVDLLAAAALVVAIALLE
                     AGRRVPAGVAWAIAALSKYTPLLLGPTLVRKRAWALLLSAALTMALLTLPFLGAGRTL
                     FLGLGAYTAHWEFNGPLFPLFRAVLGSGDAARLLLAALLVVAVTAISIRARTVSGAAL
                     ACLTAYLIASPTVFPWYLVPLAALLPLHPDAGLLAATGLVALTYLPLPHFRATGEWTL
                     PSWIPWIEYGVWALVALAAWLRTRAAATPAGSQERREAWATESPPT"
     gene            complement(11781..13265)
                     /locus_tag="VFR25_04625"
     CDS             complement(11781..13265)
                     /locus_tag="VFR25_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940363.1"
                     /translation="MMVAVNHTLTDLGHASGSNAILWFHLAVWIVLAAYGLHRLHLIC
                     LYRKRRPTAVPPEPAEWPRVTIQLPIYDERYVAERLLEAAAAVDYPRERLEIQVLDDS
                     EDETAGRMTRAVAALRDRGVDAVHIRRGNRAGYKAGALEHGLARAKGDLIAIFDADFV
                     PQPDMLKRMVPHFADPRVGMVQARWKHLNRDYSLLARLQAISLDAHFLVEHTARMSAD
                     RYFNFNGTAGILRRTCIEDAGGWQSDTLTEDLDLSYRAQLKGWRFVFAGDVGCPGELP
                     VEMNAFKAQQHRWVRGSIQVARKLLPRIWKSEAPLGVKLEATFHLTSNVPYVLLLLLS
                     LIVYPVVLARYSSRSVFFLAADSVLLIAATAPVLAYFAQVERGSALKRLRWVPLVMTL
                     GIGLAVNNTRAVLEGLFCRAGAFHRTPKFRLEARRDRWRGKRYRSPLRVHVLLELAFG
                     LYFAWAMLALARAGLWAPLPFFVLYLFGFLYVGGLSVAHASRRS"
     gene            13333..15399
                     /locus_tag="VFR25_04630"
     CDS             13333..15399
                     /locus_tag="VFR25_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9940364.1"
                     /translation="MRRAALGEGREAGRHTLARAFVVGNGSVLATADISGSLRELFLP
                     SLSPGHQILRRPARIGLLLDGTLRWIDDSFAARLGEGGDAPIVDLALASEELEIEVWQ
                     ETYVDVLLDAIVRRVQVTNRAERTRDLRVVFHHDLALAPGEPRETVQWDAESRAVVHR
                     AGRRVALIALETADGSGVPMARVASRASASAAGAEALPGGVRLEGPAEASGLCDSIAG
                     APLPLAPGASGLVTAWIVFAPTLREARKKDAALRKGGVPQSLARTRGHWNLWVSQGSR
                     DLPDLPEDVQALYHRSLVLLRLHQEPSGSIGSDLEAPGTAGTDGYAPRTCRHRDAAIA
                     ADALGRAGYHGATRRYLEHAARAAREIGVIPPATDADGAPVGPGGDPAGPALVLWAAA
                     RHFERERDVEFTAPFFRDAVRPTADLLASSLDPATALPESPDLWDERGGSHAWTAVVV
                     RSGLLGAARLAAAFGDAPRARAWAGAADTIARSMTKHLFRSEWGRFATSLARDGRSWR
                     AESRLDASLLWLGLLGEAEAEDARVRATVDAVKESLWVRTGVGGIARYERDPVDAVGT
                     DLPDVPGSPSVAATLWLAQHGIRTARRAQDLDPARTLLLWCAARAEGWGALPERLHPY
                     RGATTSAAPSLQAHAWLVSTVVDYAERLRTLRRCERCGAPAGTGREKRVAALLDPLAP
                     GLVAHS"
     gene            complement(15370..16608)
                     /locus_tag="VFR25_04635"
     CDS             complement(15370..16608)
                     /locus_tag="VFR25_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940365.1"
                     /translation="MRALTVTRAARMGLGLAALLCLTLAAPSARANSVFSSAGLGEPT
                     LEESARLRALGGAGAAEHGPSNFSLVNPASIAEAEHLILEATLLQTRRQVTTLHYGDE
                     TGNESSFPSVRLVLRLPNGLVLGGSYLIGTNGEFQVTRPESTGAASVLRIDGRGGINF
                     LRVTAAAKVGRSLRAGVDYEVAGGSFHEEWQRQFVDPNLAANRDTLDVSWDRLGRWRF
                     GAQWVKSWISLGAAYETGRRLPLTERQKAAGSLTETANSLGLPEGIVLGFELKPRERT
                     RFVGQYRRQNYGESTIQSDLVTFRPLERYSIGWERMPVNGGRAGLGLQRIPLRLGFMV
                     LRWPDLLPVAGATDIGGGAAAVEEWAASIGTGLRTKDGGGAIDLSLEGGQRGDKDKLG
                     AMETFFRLGLSLRVSDETWR"
     gene            complement(16608..>17256)
                     /locus_tag="VFR25_04640"
     CDS             complement(16608..>17256)
                     /locus_tag="VFR25_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940366.1"
                     /translation="TLMLQVSAIQGFAAFSSRTARLRIPFTKQVAGVTVPDTVSTTVT
                     FDLYLHPPLVPTATGSDTALVLGGPFESSIAIRGPIPAIGPGASINDLRLVFGVIDSI
                     PGYAAASDSGAVVPFTIEIYQVTGPWSETATDRSQVPTGSKPVALVVRSFPVGDTLSI
                     PLPPDLARGWTTLNEGVVLSIRNASVKQGLRLGSRESAVLPLLRVGTTSAPPGRF"
BASE COUNT         2543 a         5902 c         5904 g         2907 t
ORIGIN      
        1 ccgtgccgcg agtaaaagga acgacgctac caacgcagcg gccaagaccg tggagttccg
       61 cgcgccctgc ggggcgcgcg cataaggggc gcgcacggga aggattagac gcacgcagtc
      121 tcctccaaca agggcaacgc aacctccagc acctgctcgg gcggaatcga ctcgagcgtt
      181 gcgtccgcgg cgcgcacgga gcgcacgcag cgccccacgg gcgcccactg cgcgggatcc
      241 gaccatccgt agatcccgac cgtcgcgcat ccggtcgctg cggcgagatg gagcgggccg
      301 gtgtcgttcc cgatgtagag cgcggcgcgc gcgaagcggg ccgcgagcat cgggatcgcc
      361 tcgccacgca cgacgggtag ggtccggccg agggcgctcg acgcgcgatc cacgcagcct
      421 tcgtcctgag gcccttcgat ccaggagatc tcgcatccgc gcgcgaggag cgctcgggcc
      481 acctccgcga atcgctccgc gggccagcgg ttcttcacct tccccgcccc ggggtgcagg
      541 tacacatgcc cgcccggagc ccgctcaagg agcgcggcat cgggcacgta ggacggaggg
      601 ggcggcaccg cggcgccggc ccccgccgcg cgcgcgagcg cgagcgagac ctcggactga
      661 tggggctctc catccgggta cgggaccatc tcggtcagcg ccgcgcgatc gcgctcgccg
      721 tagcgcgcgg cttcgaagcc gatccgtcgt gtggcgcccg acgagcgcac cagccaggcg
      781 gcgctcttcg agtacgacac ggtggtgaag tagatcgcga gatcggcgcg cgggccgctc
      841 cgcgtgagcg cccccacgag cgcccccacg cccttcgtag gcaccacgcg aacgcgatcc
      901 acccaggcct ggccgagcac ggcgggggcg ttctggggtc cgacgacgag cgtgatgtgg
      961 gcgccggggt aacggtcgcg aagcgcgcga atggcgggaa cgttgaggag gagatcgccg
     1021 agctgattct ggggccggta gagctcgatg cgccggggtt ccaaatcgga cccgcccggg
     1081 gtcattcctt gccgggggtt cgcttgagct tctccagctc gagcaccttc acgaggcggt
     1141 catggcaatc cgccggcatg gagggcgttt cgaagtgtcg atagagcttg atggtcaacg
     1201 tgagcgtgtc gaattcggcg cggcagtcgg tgcagagtcc gatgtgcttc ttcatctcga
     1261 cggcgacttc ctgcttcaga tcctcatcga gatacccgga gagtgattcg aggacctcct
     1321 tgcacgtcat catcactggg ctcccatggc gcgccgcgtt cgcggctcgg tgaagtaggt
     1381 cgcgagcctg ttgcggacct tgaggcgcgc tcggtgcagc ctggacttca cggccgccac
     1441 cgagagatcg aggatctcgg ccacttcggc attggacagc tcctcgatgt cgcgcagcac
     1501 gaacacctgg cggaattccg ggggaagggc atcgatggct tcggtcatcg cggcttgcgt
     1561 ttcctggttg agcatctgct cgaccggacc cggggcccag tctgcgatat tgatcggcgc
     1621 ttcccgctcg tcggcgtcgt cgatcgacac cgtcggcagc tgtcgcttgc gaaggcgcat
     1681 gagcgccgag ttcgccgtga tccgatagat ccacgtcgaa aacgtggaat tgcccttgaa
     1741 ggacttgatg ccgcgatacg cgcgaaggaa cgtgtcctga agcacgtcct ccgcgtcctc
     1801 cggattgcgg agcatcttca gggccaggct gtacaccttc gactcgtagc gggacaccag
     1861 ctcgccgaac gcacgctcgt tgtcctgttg agacttccgg acgagctgtt cgtcggtctc
     1921 tgcttcggtc acgtcttcct cgcccctatc gatgcgtggg agcaaacggt gggttcccag
     1981 aaaagttcct gaatgaggct acggaccccc cgggggggtg tcaaggcgag aaggcggggc
     2041 ctaaacggcg ccgacccgga cgggctcttc gcgcccgagg ccgtggcggc gcatcttgca
     2101 gcggaggctg ccgcggccga tgtggaggag ccgggcggct tccaccacgt ttccgtgcgt
     2161 ccgggccagg gcaccgagga tcacggcctt ctcgaccgcg acgagggaga ccccctcgga
     2221 tgggatccgg aggttcaggt cggacaggcc gatttcctgc cccgaggcgt cccggggctt
     2281 cgggatcgcg agctcgtcgg gtccgatctc ttccgtgcgc gcgaggagca cggcgcgctc
     2341 gatcgcattc atgagctccc ggacgttgcc gggccagccg taccgcgcga tgtccttctc
     2401 ccccgcggag ctcaggtgcg ccttcttgcc gagcttccgc ccgacgcgct cgacgaagag
     2461 gcgcgcgagg agcgcggcgt cgtcgccgcg gtcgcgaagg ggcgggatgg agatcgggac
     2521 cacgctgaga cggtagtaga gatcctcgcg gaagcgaccc tgccgcacct cgtcgacgag
     2581 cgagcgattg gtcgcggcga ggatgcgcac gtcgacgggg atgtcgcggt tgccgcccac
     2641 gcgccgcacg atcttcgaat cgaggaactt gaggagcttc gcctggagcg tgagcggcag
     2701 ctcgccgatc tcgtcgagga agaccgagcc gtgctcggcc gcttcgaaga gacccacccg
     2761 ctgctgcgtg gcgtcggtga acgccccttt ctcgtgtccg aagagctccg actcgaggag
     2821 ctcgcgcggg atcgcggcgc agttgatctc gaggaacggc ccgtgcgaac gcgggctctc
     2881 gccatggatg gcgcgcgcga agagctcctt gcccacgccg ctctcgccga gaaggagaac
     2941 ggaggtcgcg ctcgattcgg cgacgctctg ggcgagcccg atcgagtcct tgagcgcggc
     3001 gctctgcccc acgatccggt cgaagcggaa ttcgtcacgg acgcgcgcgg tctcgcgctg
     3061 ctggcgggcc tgctgcgcgg cactcttccc cgccgccttg aggaggccgt cgatcgggtc
     3121 gaagcggacc ggcttgaggt agagattccg gatcccgagc tcgttcatct cggccgcttc
     3181 ggcggcggag ctgcggtcga gccagagcgc gagctggagg cgcggatagc gctccttgac
     3241 gcgcttgatc gcgtcgcggc gctccgcgtg atcgggaccg ccgttctcgg aatcgccgaa
     3301 atccaggaga gccagcccgc aggaatccgc ctgggcgtgc gccaggaact cggccacgtg
     3361 ctcggtgacg agcaccgggc accccgagtc ctcgacgtgc tggaccaggc gctcccgctc
     3421 ggacccggcg ggcatgatga tgccgacgtt gagattcaag gtgttacgac ccccacgacc
     3481 ctgggatggg acggacgacc cgacgccttt gctatcggcg tggagggggg aaacgtttag
     3541 ggggaagtcg gctgggctac gggaggttgc cggccctggc cggcggcggg gcgagggggc
     3601 tagcgggcgg gctggggagc cttcacggtc gccgggaagc gcatccggag caccagctcg
     3661 gcaaatctcg gatcgggacg gagacggtcc catcgcggat cgacgccgag caggaccatc
     3721 atgttggcgc ggacctcgaa gccccgatcg agctgcgcga acgcctcgtc gtccaggccc
     3781 agcgcctcgc acacgagggc cagatcgaac ggcgaaacgt agcgccgcga ggcaaggtcg
     3841 cggagctcgt tgagaatcgc ctccgcttcg gtccggcgcc ccatcttcgc gagcgcccgc
     3901 cccatgccgg agagggccca tgggttcctc gcggtcatct ccccgccgcg gatcgcgacg
     3961 cgaagcgcct tctcgtattc gccccggact tcgtagatgg gcgacgccca caggaaggcg
     4021 gtcgagaagt cgggctcgcg atccatgagt tcctcgatga accggacggc gtcatcgaca
     4081 tgccctccga agaagtgcac ccaagctcga gccgcgcgaa tgatcatcga gagcggatcg
     4141 agctcgtggc cgcgcgtcac cgtccggatg gattcctcgt aacggcccat tgcgatgagc
     4201 atgttcgcgt agaacaggtg gatgtacgcg ctgccctcgt tcagctccag cgcgcgacgg
     4261 aagctccgct cggccgccgc ccagtcccac tcgtagtaat gctgcacgta tccgagcgcc
     4321 gagtaggcct cggcgagccg gtcgtccatc cggagcgccg cgttcgcagc aatcttcgcc
     4381 ttgggaaagc tgtcccccgg cggcagcgtg ccgtagaagc cgatcacgca gtaggcgtcg
     4441 gcgaggcccg cgcgggcggt cgcccattcg ggatcgaggt cgatcgcctc gcggaagtac
     4501 tcgaggctcc ggagcaatcc ctcgtcgttc cgcttgttcc agtgctgccg gccgcgcagg
     4561 tagctctcgt acgcgcgggg gtcgacgacg tgcgtccgag cgagacgcgc ctcctcctcg
     4621 ggcgtcacct tcacgcggat ctcctgcgcg atcgcgcgcg cgacctcgcc ctggagcgcc
     4681 aggacgtctt tgagatcgcg ctcgtagctc ttggcccaga gatggcggtc cgtcgcgacc
     4741 tcgatgagct gcgccgtgat gcgcacgcgg tccgcgtgcc gtcgcacgga gccctccacg
     4801 atggcttcga cattgagctc gcggccgatc tcctggagcg tcttcgtcgt cttcttgtag
     4861 cgcatcgcgg acgtgcgcga gatcacgcgc agcgcctgga tctgcgccag atccgcgatc
     4921 agctcctcgg tcatgccgtc ggcgaagaac tcctgctccg gatcgccgga gagattctcg
     4981 agcggcagga ccacgaggcc gcggatggat ggagcggtcg aggtcggggt cgccgaggtg
     5041 ccgtcccctt tcagctcggt gatgacgtcg cgcgcggttc gaggtcggcg gtcggcctcc
     5101 ttctcgagga ggcgggcgac gagcgcctcg aggcgcggcg gcaccgggcg caggcgcgtg
     5161 aggggctggg ggcgctcggt gaggatggcc ttcatgagcg gtccggctcc catcgcggtg
     5221 aagggccgcc gtccggcgag catctcgtag aggagcgcac cgagcgcgta gagatcggcg
     5281 cgtccatcca cgtccttccc ctcgagctgc tctggagcca tgtaggggag cgtccccgac
     5341 caggagcccg catccgtgag cgccatcgta tcggcggcat cgctcgtctg caggaggcgc
     5401 gcgagcccga agtcgagcac cttcacgcgt cccttcggcg tgatcgccac gttggccggc
     5461 ttcaggtcgc ggtgcacgac gccctgctcg tgcgcctcct cgagcgcctc cgcgatctgg
     5521 atcgcgatgg gcgtcgcgtc ggcttcggag agggagcccc ggaggaggcg ctgcgccagc
     5581 gtctccccct ccacgtgctc catgacgagg aagtcgatgc cgtccgcaca gtcgaaatcg
     5641 tggacggtcg cgatgttcgg gtggctcaag cgcgacaggg ccatcgcttc ccggcggaag
     5701 cggagccgcg cggattcgtt cgtgaggatc gcgggggcga gcaccttgac cgccacgtcg
     5761 cggttgaggc gcagatcacg ggcgcgatag acgacgccca tcccgccgcc gccgaggcgg
     5821 tcgatcagcg tgtagtgggt gagtgtcttg ccgagcacct ggcccccgtg gatcccgcgc
     5881 tcccgaaacc gcggatgcgg ttcgggggcg gaatcgtatc acggagggcc ggaagccgcg
     5941 cgtggcgcgg cggcgcgcct tacttcggca tcacgtggca ggtgggcgcg atgccgcgct
     6001 tctcgaaata gcgctggtgg tactcctcgg cgggatagaa cggcgcggcc ggcaggatct
     6061 gggtcacgat cgaacggcgg aagcgcttcg cctcggtcag cttgtccacc gcttcgtgcg
     6121 cctggcggtc ctgctccgga gaatgtgtga agatcgccga gcgatactgc tcgcccacgt
     6181 ccggtccttg gcggttcggc gtggtcgggt cgtggttctc gaagaacacc tgtacgagag
     6241 tctcgtacga gacgcgctcc gggtcgtacg tcacctcgac ggcttcggca tgcccggtgc
     6301 gtcccgtgca cacctgctcg taggaggggt tgacggtgcg tccgccggtg tagccgacca
     6361 cggcgtcgag gacgcccggg atcgcgcgga acatcgactc cactccccag aagcacccgg
     6421 cggcaaacgt cgccttcgcg ggctcacggc tggtcatgac tggctcctct ctctcgaatg
     6481 ggtacggctt cgggtctcac acgagagtac gccgcagcaa cggtgttgag accgaaaaac
     6541 ggagcgcccc gggcttgact cccggggcgc tggtccgttt tgactctgaa cggggccgct
     6601 tgcgcgactt cgttacttca ccacttcgta atccgcgtcg acggctccat cgccgccttc
     6661 gcgcttgtcc tggggcgcgc cctgcggctc accctgaggc ggctcctgcc cgccctggcc
     6721 cttcgacgcc tcggcgtaca gcttgctcga gacctcgttc cagacctggg tcaaggcttc
     6781 gctcgcgctc cggatctcgg tggtgtcgtt ccgctcgacc gccttcttca gcgtctcgag
     6841 cgcgctctcc accttgctcc tcgactcggc gtccaccttg tccccgagct ccttgagatt
     6901 cttctcggtc tcgtaggcca gcgcgtcgcc gcggttcttc acctcgatgt cctcgcgccg
     6961 cttcttgtcc tcatcggcgt gggattccgc atcggacacc atgcgcttga tctcggtgtc
     7021 gctcagacca ccgctcgcct cgatcttgat cttctgctcc ttgccggtcg ccttgtcgcg
     7081 cgccgccaca tggaggatgc cgttcgcgtc gatgtcgaac gtcacctcga tctgcgggag
     7141 gccgcgcggc gccggcggaa ggccggtgag cgtgaagcgc ccgatggtct tgttgtcctg
     7201 cgccatctgc cgctcgccct ggagcacgtg gatctccacc tgcgtctggt tgtcggacgc
     7261 cgtcgagaag gtctcgccct tcttcgtcgg aatcgtggtg ttccgctcga tcagccgcgt
     7321 ggtcacaccg cccagggtct cgatgccaag cgagaggggc gtcacgtcga ggagcagcac
     7381 gtccttgacg tcgcccgcga ggacgccgcc ctggatggcc gcgccgatcg cgacgacctc
     7441 gtccggattg actcccttgt gcggctcctt gccgccgaac agcttcttca ccagctcctg
     7501 caccttcggc gtgcgcgtcg cgccgccgac caggatcacc tcgtcgatgt cggaggcctt
     7561 gagccccgcg tcggcgagcg cccgctccac cggccccttg gtgcgctcga tcagatcgtc
     7621 cacgagctgc tcgaacttgg accgggtgag ggtcaggttc aggtgcttcg gaccgctcgc
     7681 gtccgccgtg atgaacggca ggttcacgtc ggtctgcatg accgtcgaca gctcgatctt
     7741 ggccttctcc gccgcctcct tgaggcgctg gagggccatc gcgtccttgc ggagatcgat
     7801 gccttcctgc ttcttgaact cgtccgcgag ccaatcgatg acgcgcttgt cgaagtcgtc
     7861 gccgcccagg tgggtgtccc cgttcgtcga cttgacctcg aacacgccgt ccccgagctc
     7921 gaggatggag atgtcgaacg tgccgccgcc caggtcgtag acggcgatct tctcgtcctt
     7981 cttcttgtcg agtccgtagg ccagcgcggc cgcggtcggc tcgttgatga tgcgcttcac
     8041 gtcgaggccc gcgatcttgc ccgcgtcctt cgtcgcctgg cgctgcgtgt cgttgaagta
     8101 agccggcacg gtgatgacgg cttccgtcac cttctcaccg agatagtctt ccgccgtctg
     8161 cttcatcttc tggaggatca tcgccgagat ctcggggggc gaatactgct tcccctcgac
     8221 gtcgatgacc gcctcgccgt tcttgccgtc cttcaccgcg tacggaacgt tcttcgcttc
     8281 ctcggccacc tcggcatagc ggcggcccat gaagcgcttg atcgagtaga tcgtgttctt
     8341 gggattggtc acggcctggc gcttggccac ctggcccgtg agccgctcgc cggtcttcgt
     8401 gaatgccacg acggaggggg tggtccgcgt gccctcggag ttcgagatca cgaccggctc
     8461 cccgccttcc atcacggcca cgcacgagtt ggtcgtgccc aggtcgattc cgatggtctt
     8521 gcccattgtc cctcccatgg ccaagcgcgg agaggcatgc gcctccccgc gccctctggc
     8581 tttttttcag acgccgatcc gagacggatc gtgcgctacg actgcgttcc ctgcgaggtc
     8641 ttgatgggga tctcgcggga cttggcctcc tcggccttcg gtacgttcac ttcgagcaca
     8701 ccgtcgcgat aggtcgcgga aatgcgctcc ggctgaaccg cgtgggacag cgtgaaggcc
     8761 cgctcgaact ggccgtacac gcgctccgtg cggatgtagt tggcttcctt cttctcttcc
     8821 tcgcgccgct tctcgccgcg aatgacgagc tggttgtcgg cgaaggtgat cttgacgtcc
     8881 tcgagcttca tgcccggaag ctcggtccgg atcgtgaagc cttcgggatg ctcttcgaca
     8941 tccacggcgg gctgccacgc cacggggcta ccctggcttc ggctcacccc cagaacctct
     9001 tcgaataggc ggttgagatc gttctgaaaa gcgaaggggt cccgcgccgg ggtccaacga
     9061 atgagtgcca tggtcgtact ctcctcctcg tgcatgaacg ttgttggctt ctctcgggag
     9121 cggttcgggc cgctccctgt tcctgccgag aggcaaagca cctgctgtgc cagaaggccc
     9181 atggaagcta tctcgctgat gctatgagat ttaacctcta tcttacccga ccacgaggca
     9241 gtgtcataat cgtacagatg acctattttg aaactcgatt cgctccataa tggaaccgag
     9301 cctagattcg atactgcttc atcttcctcc acagcgtcga gcgggaaatt ccgaggctcc
     9361 gagccgtgcg ggtcgtgttc cccgcgaagt gggagagcgc acggcggatg tgctcctgct
     9421 cgatctgggc caggctcatc gtctccgagt agggaaacgc gttcccctcg cccagcacga
     9481 ggacggacgg ctcgcggaag gaagggggga gatccatgtg gccgatctcg ggaccctcgg
     9541 cgagggtcac cgcccgctcg atcgcgttct cgagctcgcg gacgttgccc ggatagtcgt
     9601 agtggaggag cgcgtactgg gctcgctcgc tgaatcgctc cacccgcttc ttcagcctcc
     9661 gcgcgaatcg ctggcggaaa tagttcgcga ggagcgggat gtcctcgcgc cggtcgcgca
     9721 gcggcggaag gtcgatgtgg aatacgctca ggcggtagta cagatcttcg cggagccggc
     9781 cctcggccag ggcgcgcttc acgtcgcgat tcgtggcggc gatcacgcgc acgtcgatgc
     9841 ggctggattc ggcgtccccg acccgccgga tctcgccgtt ctggagggcg cgcagaagct
     9901 tgggctggac ggagagcggc atctcggcga cctcgtccag gaagatcgtg cctccgtccg
     9961 cgcgcttcag gaggcccacc ttgtcggaga tcgcaccggt gaaggatccg cgcacgtggc
    10021 cgaagagctc gctctcgaac agcgtttccg gaatggcggc acagttgacg gtgacgaagg
    10081 gcttgtgggc gcgggcactc tggagatgga tcgaggcggc caccatctcc tttccggtgc
    10141 cgctctcgcc ggtgatcaag accgtcgact ccgtcggggc caccttctcc accaggcgga
    10201 acacgcgctg catccgggga ctgaccccga cgatctcctg cgtgatcccg ctcggcttcc
    10261 gctcctcgtc cgtctggacg gtcgcctccc agaggccgcg cgacgcggga tctcgcgcct
    10321 cgagctccgc cgcggaatcg gccgtgcgcg cggcgcgcga cgcctcctcc acgccggcgt
    10381 cgactcggga cccggatcgt tgaccgatcc gccccgcttt cctaggatgc gactcgcccg
    10441 ttcgactcac ggacgaggga attccaccat cgtgcatcgc acatcctctc catcattcaa
    10501 aaacagccca tgattctctt cttcatttct ggaattgtgc tcgtcgcgtt tgcggcggtg
    10561 cttccacggc tcggcgatct gggatcgaag gcgcacctgc tcctctatct ctgggcgatc
    10621 gcccacgccg cctatctcct ggccgcgtat cgcgcgctcc gggcccctgc gggtgccgcg
    10681 tccaagcgcg cactcggctg gatcctcggc atggcgctcc tggcccggct ccttctcatt
    10741 ccggccgcgc ccacgctctc cgaggacgcg tatcgctatc tctgggacgg ctcgctgacg
    10801 cgagacggca tcaatccctt cgttcacgcc ccctccgatc ccgcgctcgc ggagcgccgg
    10861 aacgacctct tcgcccggct caaccacgcc gacgtcccca cgatctatcc gcccgcggcg
    10921 cagctcttct tcggcgccgt cgcatcggtg cgtgcgacgc cggccgcgct caaggccgcg
    10981 tacctcccga tcgagatcgc ggcctggatc gcgcttctcg cgctcctgcg gcgccgcggc
    11041 ctcgctcccg aacggatcct cctcttcgcg tggaatccgc tcgtcttggt cgagagctac
    11101 gggagcggcc acgtggatct cctggccgcg gcagctctcg tcgttgcgat cgcgctcctc
    11161 gaggccggcc gccgtgttcc ggccggcgtc gcgtgggcca tcgccgctct gagcaagtat
    11221 acgccccttc tcctcggtcc cacgctcgtc cggaagaggg cctgggcgct cctattgtcg
    11281 gccgctctga ccatggcgct cctcacgctc cccttcctcg gagcgggacg gacgcttttc
    11341 cttggactag gggcctacac ggcgcactgg gagttcaacg gacctctctt cccgctcttc
    11401 cgggcggtgc tcggctcggg cgatgccgcg cggctcctcc tggcggcgct tctcgtcgtg
    11461 gcggtcaccg cgatctcgat tcgcgcgagg accgtgagcg gcgccgcgct cgcgtgcctc
    11521 acggcctacc tcatcgcgag ccccaccgtg tttccgtggt acctcgtgcc gctcgcggcg
    11581 ctcctgccgc tccatccgga cgcgggcctc cttgccgcga ccggtctggt cgcgctcacg
    11641 taccttcccc tgccgcactt ccgggccacc ggcgagtgga cgctgccgtc ctggatcccg
    11701 tggatcgaat atggagtctg ggcgctcgtc gccttggcgg catggctaag gacgcgcgcg
    11761 gccgcgaccc cggccggatc tcaggagcgg cgcgaggcgt gggccaccga aagcccgccc
    11821 acgtagagga atccgaagag gtacagaacg aagaagggaa gcggcgccca gaggcctgcg
    11881 cgagcgagcg ccagcatcgc ccatgcgaaa tagagtccga acgcgagctc gaggagcacg
    11941 tgcacgcgaa gcggcgaccg gtaccgcttg ccgcgccagc ggtcgcgccg cgcttccagc
    12001 cggaacttcg gcgtccggtg gaaggctccc gcccggcaga agagaccctc gaggacagcc
    12061 cgcgtgttgt tcaccgcgag cccgattccg agcgtcatca cgagaggcac ccaccgaagc
    12121 cgcttgagcg cggagccgcg ctcgacctgc gcgaagtagg cgaggacggg ggcggtcgcg
    12181 gcgatgagga gcaccgagtc cgccgccagg aagaagacgc tccggctcga gtagcgcgcg
    12241 agcacgaccg gatagacgat gagcgagagg agaaggagca gcacgtacgg gacgttggac
    12301 gtgaggtgga acgtggcctc gagcttcacg ccgagcggcg cctcgctctt ccagatccgc
    12361 ggaaggagct tgcgcgcgac ctggatcgag cctcgaaccc accggtgctg ctgcgccttg
    12421 aacgcgttca tctccaccgg gagctcgccc gggcagccga cgtcccccgc gaatacgaac
    12481 cgccatccct tgagctgggc gcgatagctg aggtcgagat cctcggtcag cgtgtccgac
    12541 tgccacccgc ccgcgtcctc gatgcacgtc cgccggagga tgccggcggt gccgttgaag
    12601 ttgaaatagc gatccgcgct catccgggcg gtgtgctcca ccaggaagtg cgcgtcgagc
    12661 gagatggctt gaagccgggc gaggagggag tagtcgcgat tgagatgctt ccaccgcgcc
    12721 tggaccatgc cgaccctcgg atccgcgaag tgcgggacca tcctcttgag catgtcgggc
    12781 tggggcacga agtcggcgtc gaagatggcg atgagatcgc ccttcgcgcg agcgagaccg
    12841 tgctcgagcg cgcccgcctt gtatcccgcg cgattcccgc ggcggatgtg aaccgcgtcc
    12901 acgccgcgat cgcggagcgc cgccacggcg cgcgtcatcc ggccggctgt ctcgtcctcg
    12961 gaatcgtcga gcacctggat ctcgaggcgt tcacgcggat agtccacggc cgccgcggcc
    13021 tcgaggagcc gctccgccac gtagcgctcg tcgtagatcg ggagctggat cgtgacgcgg
    13081 ggccattccg cgggctcggg cgggaccgcg gtgggccggc gctttcgata cagacagatg
    13141 agatggagcc ggtggaggcc gtaggcggcc agcacgatcc acaccgcgag atggaaccag
    13201 agaatcgcgt tcgatcccga ggcgtggccg aggtccgtga gcgtatggtt caccgcgacc
    13261 atcatgaaac ctgccggaat cgtgtcaagt cgaactcgcg atcgttgacc gcggggcgcg
    13321 cgctctgtta gcgtgcgacg cgccgcattg ggagagggtc gggaggctgg gaggcacaca
    13381 ctggcacgcg cgttcgtcgt cggaaacgga tcggtcctcg ccacggccga catctcgggc
    13441 tcgctccgcg agctcttcct accctcgctt tcccccggtc atcagatttt gcgccgtcca
    13501 gccaggatcg gtcttcttct ggacggcacg ctccgctgga tcgacgactc gttcgcagct
    13561 cggctcggag agggcggcga cgctccgatc gtcgatctag cgctcgcgtc cgaagagctc
    13621 gagatcgagg tgtggcaaga gacctacgtc gacgtgctcc tggatgcgat cgtccgacgc
    13681 gtgcaggtga ccaaccgcgc ggagcgcacg cgcgacctca gggtcgtctt ccatcacgat
    13741 ctcgcactcg cgcccggcga gccgcgcgaa accgtccagt gggacgccga gagccgcgcg
    13801 gtcgtgcatc gcgccggacg gcgcgtggcc ctgatcgctc tcgagacggc tgacggatcc
    13861 ggcgtgccca tggcgcgcgt cgcctcgcgt gcatccgcga gcgccgccgg ggcggaggcg
    13921 ctcccgggag gcgtgcgtct cgaaggtccg gccgaggcga gcggactctg cgactcgatc
    13981 gccggcgctc ccctgccgct cgcaccgggt gcgtcggggc tcgtgacggc gtggatcgtg
    14041 ttcgccccca cgcttcgcga agcgcggaag aaggacgccg cgctcagaaa ggggggcgtg
    14101 ccccagtccc tcgcgcggac gcgcgggcac tggaatctct gggtaagcca gggctcgcgc
    14161 gacctcccgg acctcccgga ggacgtccag gcgctctacc accggagcct cgtgctcctc
    14221 cgcctgcatc aggagccgag cggctcgatc ggatcggacc tcgaagcccc gggcacggcc
    14281 ggcaccgacg ggtacgcgcc gcgaacctgc cgccatcgcg acgccgcgat cgcggcggac
    14341 gcgctcggac gcgcgggcta tcacggcgcg acgaggcgct atctcgagca cgcggcgcgc
    14401 gcggcgcgcg agatcggtgt gattcccccg gcgaccgacg cggacggcgc tcccgtcgga
    14461 ccgggcggcg atcctgcggg tccggcactc gtgctgtggg cggccgcgcg ccacttcgag
    14521 cgggagcgcg acgtggagtt caccgccccg ttctttcgcg acgcggtgcg ccccacggcg
    14581 gacctcctcg cgtcctcgct cgatccggcg accgcgcttc cggaatcgcc cgacctctgg
    14641 gacgagcgag ggggaagcca cgcctggacg gcggttgtcg tgcggtccgg cctcctcggc
    14701 gccgcgcgcc tcgcggccgc gttcggcgac gcgccgcgcg cccgcgcgtg ggccggagcc
    14761 gccgacacga tcgcgcgctc gatgaccaag catctcttcc gttcggaatg gggacgcttc
    14821 gcgacttccc tcgcgcggga cgggcgctcc tggcgtgcgg agtcccggct cgacgcgtcg
    14881 ctcctctggc tcgggctcct cggcgaggcc gaagcggagg acgcgcgcgt gcgcgcgacg
    14941 gtggacgccg tgaaggaatc gctgtgggtg agaaccggcg ttggaggcat cgcgcgctac
    15001 gagcgggatc ccgtggatgc ggtgggcacg gatctgccgg acgtgcccgg aagcccgtcg
    15061 gtcgcggcca cgctctggct cgcgcagcac gggatccgaa ccgcgcgccg cgcgcaagac
    15121 ctcgatcccg cgcggacgct tctcctctgg tgcgcggcgc gcgcggaagg ctggggagcg
    15181 ctccccgaga gactccatcc ctaccgcgga gccacgacct cggcggcgcc gtcgctccag
    15241 gcgcacgcct ggctcgtctc gacggtggtg gactacgcgg agcgtctccg cacgctcagg
    15301 cggtgcgagc ggtgcggggc gccggcggga acggggcggg agaagcgggt tgcggcgctc
    15361 ctggacccgc tagcgccagg tctcgtcgct cactcgtagc gagaggccga gccggaagaa
    15421 agtctccatc gcgcccagct tgtccttgtc gccccgctgt cctccctcga ggctgagatc
    15481 gatcgcgccc ccaccgtcct tcgtgcgaag gccggttccg atcgaggccg cccattcctc
    15541 cacggcggcc gcgcccccgc cgatgtcggt ggcgccggcg accggaagta gatccggcca
    15601 gcggagcacc atgaagccga ggcgaagtgg aatgcgctgg aggccgagtc cggcgcgtcc
    15661 cccattcacc ggcatccgct cccagccgat cgagtaccgc tcgagagggc ggaaggtgac
    15721 gagatcgctc tggatcgtcg actcgccgta gttctgacgc cggtactgcc ccacgaatcg
    15781 agtgcgctcg cgcggcttca gctcgaaacc aagaacgatg ccctcgggaa ggccgaggga
    15841 gttcgcggtc tccgtgagcg agccggccgc cttctgacgc tcggtgagcg ggagccgccg
    15901 tccggtctcg taggcagccc cgagcgagat ccaggacttc acccactgcg ctccgaagcg
    15961 ccaccggccg agccggtccc aggagacgtc gagcgtgtcg cgattggcag ccaggttggg
    16021 atcgacgaac tggcgctgcc actcctcgtg gaagctgccc ccggcgactt cgtagtccac
    16081 ccctgcccgg agactccggc cgaccttcgc ggcggcggtg acgcggagga aattgatccc
    16141 gccgcggccg tcgatgcgaa gcaccgacgc ggcccccgtg ctctccggcc tcgtcacctg
    16201 gaattcaccg ttcgtcccga tcaagtagct cccgccgagc accaggccat tggggagccg
    16261 cagaacgagg cggaccgagg ggaacgagga ctcgtttccg gtctcgtcgc cgtagtggag
    16321 cgtggtgacc tggcggcgcg tctggaggag cgtcgcctcg agaatgagat gctccgcctc
    16381 ggcgatggag gcgggattga cgagtgagaa gttcgaggga ccgtgctccg ccgcacccgc
    16441 gccgccgagg gctcgaagcc gagcgctctc ctccagcgtg ggctcgccga gcccggccga
    16501 ggagaagacg gagttggcgc gcgcggacgg agctgcgagc gtgaggcata gaagcgccgc
    16561 caggccgagg cccattcgcg ccgcgcgcgt gaccgtgagc gcgcgcatca gaagcgtccc
    16621 ggcggcgcgc tggtcgtacc cacgcgcaag agcggcagga ccgcggactc gcgcgaaccg
    16681 agcctgagcc cctgcttcac gctcgcgttc cgtatcgaga ggacgactcc ttcgttcagc
    16741 gtggtccagc cgcgcgcgag atcgggcggg agtgggatcg agagggtgtc tccgaccggg
    16801 aacgagcgca ccacgagggc gaccggtttc gatccggtcg ggacctggga acgatcggtc
    16861 gcggtctcgg accatggtcc ggtcacctga tagatctcga tcgtgaacgg caccacggcg
    16921 ccgctgtcgc tcgcggccgc atacccggga atcgaatcga tcacgccgaa gacgagcctc
    16981 agatcgttga tggaggcccc cgggccaatc gcgggaatcg gcccgcggat cgcgatgctc
    17041 gactcgaacg gaccaccgag gacgagcgcg gtgtccgatc ccgtggccgt tggaacaagt
    17101 ggcgggtgca ggtagagatc gaaggtcacc gtcgtgctga cggtgtcggg gacggtcacg
    17161 cctgccacct gcttggtgaa cgggatgcgc aggcgcgcgg tgcgcgagga gaaggccgcg
    17221 aagccctgga tcgcggacac ctggagcatc agggtg
//
LOCUS       DASTKC010000113         8552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1503153, whole genome shotgun sequence.
ACCESSION   DASTKC010000113 DASTKC010000000
VERSION     DASTKC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503153"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VFR25_04645"
     CDS             <1..1503
                     /locus_tag="VFR25_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9940367.1"
                     /translation="PRNPRDYSPRREPDRALRRRNLVLAAMLDNKRLTRSQYEAASEA
                     PLGVTKTRIDTKAAPYFMEMVRQYVEQRYGSNQLYEGGLKIYTTLDIDLQMAAEEALE
                     KRLTALEARNQYKRTRAALAAAAAKAKNPEDPKTKGKTDYLQGAIVSLDPTNGQIRAL
                     IGGRDFNESNFNRAVQASRQPGSAFKPFIYAAAIDNGFSPTDVILDTPVSFKAGNGMD
                     WSPQNYDHKFRGPVTLRSALAHSVNVPAAKLLQKLGTGVVTSYARRMGVRSRLGNDLS
                     LALGTSEVNLLELTSAYGVFANQGIRVPPSYVLKIEDKNGKVLEQTRLTAEEVLSPET
                     ALTMTSMLSSVIENGTAAAAHALGLNVPAAGKTGTTDDYSDAWFVGFTPSLVTGVWVG
                     FDQKQKIGPGMTGAAAALPIWVDVIGAATKGKAPQEFPVPSGVVSVLICTQTGLLANP
                     SCPETEYELFHEGLQPTSYCDVHTGQTPTPENYDLQKTDSEAPPEERLRL"
     gene            complement(1493..2110)
                     /locus_tag="VFR25_04650"
     CDS             complement(1493..2110)
                     /locus_tag="VFR25_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940368.1"
                     /translation="MRRTAAIRVAPATPDRWDDLEQLFGPRGACAGCWCMWWRLRRSE
                     WSQGKGEGNRRALRALVRSGEPSGLIAYSGKEPVGWIAVAPRAAYPGLDRSRTLKPVD
                     ERPVWSVTCFFTAREHRRRGVTVKLLEAAAKHARSRGADCLEGYPVEPRKDSVPDVWV
                     FTGLAGAFRKAGFVEVARRSETRPIMRRELRAKPRASRGRKAARA"
     gene            complement(2107..3399)
                     /locus_tag="VFR25_04655"
     CDS             complement(2107..3399)
                     /locus_tag="VFR25_04655"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9940369.1"
                     /translation="MKELIRRKRDGEALTPEEIERWIRGVVDGSVPDYQSSALLMAIT
                     LRGMTRAETIALTESMLRSGRVLEWSRLGRPTVDKHSTGGVGDKVSLALAPWVAACGA
                     VVPMIAGRGLGHTGGTLDKLEAIPGFRVKLSIDEFTAQVERIGLVIAGQTAELDPADQ
                     ELYALRDVTATVESIPLIVASILSKKFASGTEAVVFDVKRGGGAFMRTEKEARALARE
                     LVDVSHELKRRGYAVITSMEQPLGQAIGNANETAEAFSLLHGQAPPDLMEVTKELAVR
                     MLLVAKIARERFEAEARLDRVLESGEAVRRAEAWVQAQGGDPRAVNDASILPRAEVET
                     PVPAPRSGYVTGIDARALGTLLVAMRGGRARKEDQVDPAVGVRLLAKRGDAVRAGEPM
                     ALVEAHRAAPEWAEAARRAFAIGDAAPAKEPVILEEVR"
     gene            complement(3433..3852)
                     /locus_tag="VFR25_04660"
     CDS             complement(3433..3852)
                     /locus_tag="VFR25_04660"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9940370.1"
                     /translation="MSARDPSGAAQAERLLAAARASIGNAIAPYSGLKVGAALEDASG
                     AIHPGVNVESVSYSLTLCAERNAVFGAVARGAREFRRLALVSNATTPILPCGACRQIL
                     YERAPDLIVIVPKDGGYEEIPIADLLPRPFRDFEPAR"
     gene            complement(3849..4100)
                     /locus_tag="VFR25_04665"
     CDS             complement(3849..4100)
                     /locus_tag="VFR25_04665"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940371.1"
                     /translation="MSDEFRVGEWVRHPEFGDGLVLEARGSGESHSVLVSFPDNSRRR
                     LMVKFAHLSKVEPPEGHHAKVPETDGKPKRARKKTSGGS"
     gene            complement(4129..5373)
                     /gene="alr"
                     /locus_tag="VFR25_04670"
     CDS             complement(4129..5373)
                     /gene="alr"
                     /locus_tag="VFR25_04670"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9940372.1"
                     /translation="MEFPTWVEVDLDRFRRNLGAIRSAIGERRRILLVVKADAYGHGA
                     VEIAHHAVSAGAAMLGVATLHEGIELRSSGLSAPIVILSPTLLGEVDEILEHRLTPSV
                     TSVEFAERLSARCVAQQLLARFHVEVDTGMGRTGVSDSEAVEFITGVVHLPNLKLEGV
                     YTHFPDADRGDTAGTEEQVRKFETILRALKLRNIDVPLRHAANSAGLLSVPDSFLDMV
                     RPGILAYGFYPTREVPRSVEVEGILSFKTRVVQLRDLPPGRNVSYGRTYTTKRWTRIG
                     VLPVGYGHGYPWQLSNRGQVLIRGQRAPIVGRVTMDLTMVDATDIEVALGDEVVLWGE
                     QQGARLGLDEVAEWAQTIPYDLLCSMGKRVVRLYVQEGSAPKVLTLIGERQEVEVAES
                     GSAAGRKRRRKVQYKTRAGTGR"
     gene            5478..6314
                     /gene="mazG"
                     /locus_tag="VFR25_04675"
     CDS             5478..6314
                     /gene="mazG"
                     /locus_tag="VFR25_04675"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET9940373.1"
                     /translation="MPRDENDPGSTSPPASDRDEFAASAAALLDLVRHLSGPDGCPWD
                     KEQTTRTLSPYVVEEAHEIADAIAADDARSAAEEIGDLLFLVTFLAVRLEREHGPSPS
                     SIARANIAKMIARHPHVFGERKDLDAQGVLRQWEAGKQREAGHASILGKSPAGLPALL
                     RAYRVQEKAASVGFDWPDVEGVLAKIREEVDETRAALATEDPARVAEEIGDLLFAVVN
                     LARFVKTDPEAHLRAATEKFKRRFDRMVDALGREGTSPERATLAEMDRLWEEAKREER
                     KG"
     gene            complement(6311..6655)
                     /locus_tag="VFR25_04680"
     CDS             complement(6311..6655)
                     /locus_tag="VFR25_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET9940374.1"
                     /translation="MNQETTAGGGTPDAAPRELTRKEMFLGLIHSFQAAAMQQMGKMP
                     SPFSQQIERDLPQARLSIDMLEMLEERTSGNLTGEEARFLKHVLTELRLNYVAEIEED
                     KKTAAKTAEPSS"
     gene            complement(6678..7853)
                     /locus_tag="VFR25_04685"
     CDS             complement(6678..7853)
                     /locus_tag="VFR25_04685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9940375.1"
                     /translation="MARIVLAKNQDRRLRSGHLWVFSNEIASIEGSPEPGGEVLVEDF
                     RGGTVGTGLYNPHSLIAVRLFARRARAIDDALLRERLEQAKALRERILPVESTYRLVY
                     SEGDFLPGLIVDRYGDYLGVQSLTLGVERRLDAILDLLMDVMKPAGICCRRDAPTRAL
                     EGLPMLEQLERGTVPDRIDAPYEGYVIEVDLRGGQKTGEFLDMRDNRKRVAQEARGRR
                     VLDLYCYTGLFSLHCAAAGAAEVVGVDRSGAAIERARRNLARNAPGKSVTFVEEETES
                     ALTRFTGDGAQFDMIVLDPPSLVKSRKAYKEGAAKYEKLNATAMRLLPPGGVLATASC
                     SHHVDAPVFLDILRSAAKRAGAAFRMVDVRGQSRDHPVLLAARETSYLTLALLERIH"
     gene            complement(7861..>8552)
                     /gene="speB"
                     /locus_tag="VFR25_04690"
     CDS             complement(7861..>8552)
                     /gene="speB"
                     /locus_tag="VFR25_04690"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET9940376.1"
                     /translation="LAEIEVEPADIEAGMRRLEEVAGEVAAQGKIPFTLGGEHSLTLA
                     PVAALLRRYPGLSVLQLDAHLDLRDSYQGTKLSHASVMRRIRDLGVSTVAVGIRAVSR
                     EEADYVHAEKAPVHLGREIRARGLDVDAVLADLGNPVYVTVDLDAFDPACVPGVGTPE
                     PGGLTWDEGLRLLRAVCERRQVVGCDVVELCPIPGQPGSDFFAAKLVNKMIGYLGLSP
                     DLKPRTSRRGA"
BASE COUNT         1346 a         3057 c         2878 g         1271 t
ORIGIN      
        1 ccgcggaatc cgcgcgatta ctcgccccgc cgcgagccgg accgcgcgct caggcgccgc
       61 aatctcgtcc tcgccgccat gctcgacaac aagcgcctca cccgctccca gtacgaggcc
      121 gcgagcgagg cgccgctcgg cgtcacgaag acgcgcatcg acacgaaggc ggccccctac
      181 ttcatggaga tggtccggca gtacgtggag cagcgctacg gatcgaacca gctctacgaa
      241 ggcggactca agatctacac gacgctcgac atcgacctcc agatggcggc cgaggaggcg
      301 ctcgagaagc gcctcaccgc gctcgaggcg aggaatcagt acaagcgcac gcgcgccgcg
      361 ctcgccgcgg ccgccgcgaa ggcgaagaac ccggaggacc cgaagaccaa gggaaagacc
      421 gattaccttc agggcgccat cgtctcgctc gatcccacga acggccagat ccgcgcgctg
      481 atcggtggcc gcgacttcaa cgagtcgaac ttcaaccgcg ccgtgcaggc gagccgccag
      541 ccgggctccg cgttcaagcc gttcatctac gccgccgcga tcgacaacgg gttctccccg
      601 accgacgtga tcctcgacac cccggtgtcg ttcaaggcgg ggaacggcat ggactggtcg
      661 ccgcagaact acgaccacaa gttccgcggc ccggtgaccc tacgctccgc gctcgcgcac
      721 tcggtcaacg tgccggcggc caagctcctc cagaagctcg ggaccggagt cgtgacgtcg
      781 tacgcgcggc ggatgggcgt gcggagccgc ctgggcaacg acctgtcgct cgcgctcgga
      841 acgtcggagg tgaaccttct cgagctgacg tcggcatacg gcgtgtttgc gaatcaggga
      901 atccgggtgc cgccgagcta cgtgctcaag atcgaggaca agaacggcaa ggtgctcgag
      961 cagacgcgac tcaccgcgga ggaagtcctg agccccgaga ccgcgctcac gatgacgagc
     1021 atgctctcga gcgtcatcga gaacggcacg gcggccgcgg cgcacgcgct cggcctcaac
     1081 gtgcccgcgg cgggcaagac ggggacgacc gacgactatt ccgacgcttg gttcgtcggc
     1141 ttcacgccga gcctcgtcac gggcgtctgg gtgggcttcg accagaagca gaagatcggc
     1201 cccggcatga ccggcgccgc ggccgcgctg ccgatctggg tggacgtgat cggcgcggcg
     1261 accaagggga aagcgccgca ggagttcccg gtgccgagcg gcgtggtgag cgtgctcatc
     1321 tgcacacaga cggggctcct cgccaacccg tcgtgcccgg agaccgagta cgagctcttc
     1381 cacgaggggc tgcagccgac gagctactgc gacgtgcaca cgggccagac gccgacgccc
     1441 gagaattacg acctgcagaa gacggacagc gaagctccgc ccgaggagcg cctcaggctc
     1501 tagcggcctt ccgcccgcgc gacgcgcgcg gcttggcgcg gagctcgcgg cgcatgatcg
     1561 gccgcgtctc ggagcgacgc gccacctcca cgaacccggc cttccgaaac gccccggcga
     1621 gtcccgtgaa cacccacacg tcgggcaccg aatccttccg cggctcgacc ggatagccct
     1681 ccagacaatc ggctccacgc gagcgtgcgt gcttggcggc ggcctcgaga agcttcaccg
     1741 tgacaccccg ccgccggtgc tcgcgcgcgg tgaagaagca ggtcaccgac cagacggggc
     1801 gctcgtccac cggcttcagc gtgcgcgagc gatcgagccc tgggtaggcc gcgcgcggcg
     1861 ccacggcgat ccagccgacc ggctccttcc cggagtacgc gatgagtccg ctcggctctc
     1921 cgctccggac gagcgcgcgg agcgcgcggc gattgccctc tcccttcccc tggctccact
     1981 cgctgcggcg aagccgccac cacatgcacc agcagcccgc gcaggccccg cgcgggccga
     2041 agagctgctc gagatcgtcc catcgatccg gcgtggcggg cgccacgcgg atcgcggcag
     2101 tcctcctcac cgtacttcct cgaggatcac cggctccttt gcgggcgcgg cgtcgccgat
     2161 cgcgaacgcg cgccgcgccg cctcggccca ctcgggggcg gcgcgatggg cttcgacgag
     2221 ggccatcggc tcccccgcgc gaacggcgtc gcctcgcttc gcgaggagcc ggacaccgac
     2281 ggcgggatcc acctggtcct ccttgcgcgc tcgtccgccc cgcatcgcga cgaggagcgt
     2341 tccgagagcg cgcgcgtcga tcccggtcac gtagccggag cgcggagctg ggaccggcgt
     2401 ctccacttcg gcgcgcggaa ggatggaggc gtcgttcacc gcgcgcggat cgcccccttg
     2461 cgcctgcacc cacgcttccg cccggcgcac cgcctcgccc gattcgagca cgcggtcgag
     2521 cctcgcttcg gcctcgaagc gctcgcgcgc gatcttcgcg acgaggagca tgcgcaccgc
     2581 cagctccttc gtgacctcca tgaggtcggg cggagcctgg ccgtggagga gcgagaacgc
     2641 ctcggcggtc tcgttcgcgt ttccgatcgc ctgtcccagg ggttgctcca tggacgtgat
     2701 cacggcgtaa ccgcgccgct tgagctcgtg cgagacgtcg accagctcgc gcgcgagggc
     2761 gcgcgcttcc ttctcggtcc gcatgaacgc cccaccgccg cgcttcacgt cgaagacgac
     2821 cgcctcggtg cctgacgcga acttcttgct caggatgctc gccacgatca gcgggatcga
     2881 ctcgacggtc gcggtcacat cgcggagggc gtagagctcc tggtccgccg ggtcgagctc
     2941 cgcggtctgc cccgcgatga ccagaccgat ccgctccacc tgtgccgtga actcgtcgat
     3001 cgagagcttc acgcgaaagc cgggaatcgc ctcgagcttg tcgagcgtgc cgccggtatg
     3061 ccccagaccg cgcccggcga tcatggggac gaccgccccg cacgcggcca cccagggagc
     3121 gagcgcgagc gagaccttgt ccccgacgcc cccggtggaa tgcttgtcca cggtgggacg
     3181 accgagtcgc gaccactcga gcacgcggcc cgaccggagc atgctctcgg tgagcgcgat
     3241 cgtctccgcg cgcgtcatcc cgcggagcgt gatggccatg aggagcgccg agctctgata
     3301 gtcggggacg gagccgtcca ccacccctcg gatccagcgc tcgatctcct cgggcgtcag
     3361 cgcctcgccg tcccgcttcc tccggatcag ctccttcatg gaagcgcggg cgtcagcaca
     3421 gcgcagcgac gatcagcgcg ctggctcgaa atcgcggaac ggccgcggca ggagatcggc
     3481 gatcgggatc tcttcgtacc ctccgtcctt cggcacgatc acgatcagat cgggggcgcg
     3541 ctcgtagagg atctggcggc atgcgccgca gggcaggatg ggagtcgtcg cgttcgaaac
     3601 gagcgcgagt cttcgaaact cgcgggcgcc ccgcgcgacg gcgccaaaga cggcgttccg
     3661 ctccgcgcag agggtgaggc tatagctgac ggattcgacg ttgacgccgg gatgaatcgc
     3721 gcccgacgcg tcctcgagcg cggcgccgac cttgagaccg gaatagggtg cgatcgcgtt
     3781 cccgatcgag gcgcgcgccg cggccaggag gcgctcggcc tgagctgcgc cggacgggtc
     3841 gcgagcgctc acgagcctcc gctcgtcttc ttgcgcgccc gcttcggctt cccgtcggtc
     3901 tcggggactt tcgcgtgatg cccttcgggc ggctccacct tgctcagatg ggcgaatttc
     3961 accatcagcc ggcgccgtga attgtccgga aacgagacca gcacggaatg cgattccccg
     4021 ctcccgcgcg cctcgagcac gagtccgtct ccgaactcgg gatgccgcac ccactctcca
     4081 acgcggaatt cgtcgctcat atgccggtcg tccctccctg gcccggttct accgtcccgt
     4141 tccggcgcgg gtcttgtact gcaccttgcg ccggcgcttc cgtccggctg cagagccgga
     4201 ttccgccacc tcgacctcct gccgctcgcc gatcagcgtc agcaccttgg gcgcgctccc
     4261 ttcttgaacg tacaaacgca cgacgcgctt ccccatgctg cagaggagat cgtagggaat
     4321 cgtctgcgcc cattccgcca cctcgtcgag gcccagacgc gccccctgct gctcgcccca
     4381 gagcaccacc tcgtcgccca gagccacttc gatgtcggtc gcatccacca tcgtgagatc
     4441 catcgtgacc cgccccacga tgggcgcgcg ctgcccgcgg atcaggacct ggccccggtt
     4501 ggagagctgc cagggatagc cgtgaccgta gccgaccggc aagacgccga tccgggtcca
     4561 gcgcttcgtc gtgtacgtcc gcccgtagct cacgttgcgt ccggggggaa gatcgcggag
     4621 ctgcaccacg cgcgtcttga aggacaggat gccttcgacc tcgaccgatc gcggaacctc
     4681 gcgcgtcggg tagaagccgt acgccaggat gccggggcgc accatgtcga gaaaggagtc
     4741 cggcacgctc aggagtcccg cgctgttcgc cgcgtggcgc agaggcacgt cgatgttccg
     4801 gagcttcaag gcgcgcagga tcgtctcgaa cttccgaacc tgctcctcgg tccccgcggt
     4861 gtctccacgg tcggcgtccg ggaaatgggt gtacacgccc tcgagcttca gattcggaag
     4921 gtgcacgacg cccgtgatga actcgaccgc ctcgctgtcg gacacgccgg tccgccccat
     4981 gccggtgtcc acctccacgt ggaaccgggc gaggagctgc tgcgcgacgc accgggcgga
     5041 gagccgctcg gcgaactcca cgctcgtgac cgacggcgtg agccggtgct cgaggatctc
     5101 gtcgacctcc ccgaggagcg tggggctcag gatcacgatc ggggcggaga ggccggagga
     5161 gcggagctcg atgccttcgt ggagggtcgc gacgccgagc atggccgcgc cggcgctgac
     5221 cgcgtgatgg gcgatctcga ccgcgccgtg gccatacgcg tcggctttca cgacgaggag
     5281 gatccttctc ctctcgccga tcgccgagcg gatggcgccg aggtttcggc ggaaccgatc
     5341 gaggtcgact tctacccagg ttgggaattc catgtcgtat cggcctaacc ggccgctcca
     5401 cccctatcat acggcgattg gggaaggcaa gcgcggggcg attggacttc cctcgaagcg
     5461 gaccctacac tcgcccgatg ccgcgcgacg agaacgaccc cggctccaca tcgcccccgg
     5521 cgtccgaccg ggacgaattc gccgcctccg cagcggccct cctcgacctg gtccggcacc
     5581 tctccggccc cgacggctgc ccctgggaca aggagcagac cacgcgcacc ctgtccccct
     5641 acgtggtcga ggaggcgcac gagatcgcgg acgccatcgc ggcggacgac gcgcggtccg
     5701 cggccgagga gattggcgat ctcctctttc tggtgacctt cctcgcggtg cggctggagc
     5761 gtgagcacgg tccgtcgccg tcgtcgatcg cgcgggcgaa catcgcgaaa atgatcgcgc
     5821 ggcacccgca cgtgttcggc gagcggaagg acctcgacgc ccagggagtg ctccgccagt
     5881 gggaagcggg gaagcagcgc gaggcggggc atgcgtcgat cctcgggaag agtcccgcgg
     5941 ggctgccggc gctcctccgg gcgtaccgcg tgcaggagaa ggcggcctcg gtgggcttcg
     6001 actggcccga cgtcgagggc gtgctggcga agatccggga agaggtggac gagaccaggg
     6061 ccgcgctcgc gaccgaggat ccggcgcgcg tcgccgagga gatcggcgat ctcctcttcg
     6121 cggtcgtgaa tctcgcgcgc ttcgtgaaga cggatcccga agcgcacctg agggccgcca
     6181 ccgagaaatt caagaggcgc ttcgatcgca tggtcgacgc gctgggtcgc gagggcacgt
     6241 cgcccgagcg ggcgaccctc gcggagatgg accggctgtg ggaggaagcg aagcgggagg
     6301 agcgaaaggg ctaggacgaa ggctccgctg tcttggccgc ggtcttcttg tcctcctcga
     6361 tctcggccac gtagttcagg cgcagctcgg tcagcacgtg cttcaggaag cgcgcctcct
     6421 cgccggtcag atttcccgac gtccgctcct ccagcatctc cagcatgtcg atcgagagcc
     6481 gcgcctgcgg caggtcgcgc tcgatctgct gactgaacgg gctcggcatc ttccccatct
     6541 gctgcatggc ggcagcctgg aacgagtgga tcagcccgag gaacatctcc ttccgggtca
     6601 gctcccttgg cgccgcatcg ggcgttccgc cgcccgccgt cgtttcctga ttcatcgcgc
     6661 gtctctcctc gcgggatcta gtgaatgcgc tccaggagcg ccagcgtcag gtaggacgtc
     6721 tctcgcgccg cgaggagcac cggatggtcc cggctctgcc cgcgcacgtc gaccattcga
     6781 aacgccgcgc cggcccgctt ggccgcggag cggaggatgt cgaggaagac gggcgcgtcc
     6841 acgtggtgcg agcaggacgc cgtcgccagg acgcctccgg gcgggaggag tctcatggcc
     6901 gtcgcgttca gcttttcgta cttggccgcc ccttccttgt aggccttgcg gctcttcacg
     6961 agcgagggcg ggtcgaggac gatcatatca aactgcgccc cgtcccccgt gaaccgcgtg
     7021 agcgccgatt ccgtctcttc ctcgacgaac gtgacgctct tgccgggagc gtttcgcgcg
     7081 agattccgcc gcgcgcgctc gatggccgcg cccgagcggt ccacgcccac cacctcggcc
     7141 gctcccgcgg ccgcgcagtg gagcgagaag agcccggtgt agcagtagag atcgagcact
     7201 cggcgtccgc gcgcctcctg ggccactcgc ttccggttgt cccgcatgtc gaggaactcg
     7261 cccgtcttct gaccgccccg gagatccacc tcgatcacgt agccttcata gggcgcgtcg
     7321 atccggtcgg gcacggtccc gcgctcgagc tgctcgagca tcgggaggcc ctcgagcgcg
     7381 cgggttgggg catcgcgccg gcagcagatc cccgccggct tcatcacgtc catgaggaga
     7441 tcgaggatcg cgtccagacg gcgctccacg ccgagcgtga ggctctgcac gccgagataa
     7501 tcgccgtagc gatccacgat gaggccgggg aggaaatctc cttcgctgta gacgaggcgg
     7561 taggtggact cgacgggcag gatgcgctcg cgaagcgcct tggcttgctc gaggcgctcg
     7621 cgcaagagcg cgtcgtcgat cgcgcgcgcc cgcctcgcga agaggcgaac cgcgatcagc
     7681 gagtgcgggt tgtagaggcc ggtgccaacc gtgccgccgc ggaagtcctc gacgagcact
     7741 tccccgcccg gctcgggcga gccctcgatc gacgcgatct cgttgctgaa cacccagagg
     7801 tgcccggacc gaaggcgccg atcctggttc ttggccagga cgatgcgcgc catgccgtgc
     7861 tcaggcgccg cggcgagagg ttcgcggctt cagatccggt gaaagaccga gatagccgat
     7921 catcttgttc accagcttgg cagcgaagaa gtcggaacct ggctgccccg ggatgggaca
     7981 aagctccacc acgtcgcagc cgacgacctg ccgccgctcg cagacggcgc ggaggaggcg
     8041 aagcccctcg tcccaggtga ggccgcccgg ctccggcgtg cccacgcccg gcacgcaggc
     8101 gggatcgaac gcgtcgagat cgaccgtgac atagaccgga ttgcccaggt cggcgagcac
     8161 ggcgtcgacg tcgagcccgc gcgcgcggat ctcgcggccg aggtgcaccg gcgccttctc
     8221 ggcgtgcacg taatccgctt cctcgcgcga gaccgctcgg atgcccacgg cgaccgtgga
     8281 gaccccaagg tcccggattc gccgcatgac cgacgcgtgg ctcagcttcg tgccctgata
     8341 cgagtctcga agatcgaggt gcgcgtcgag ctggagcacc gagagcccgg gatagcggcg
     8401 gaggagggcg gccaccggcg ccagggtgag cgaatgctcc ccgcccagcg tgaacgggat
     8461 cttcccctgc gccgccacct cgccggcgac ctcctcgagg cgccgcatgc ctgcctcgat
     8521 gtccgccggc tcgacctcga tctccgcgag cg
//
LOCUS       DASTKC010000114         6463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_85132, whole genome shotgun sequence.
ACCESSION   DASTKC010000114 DASTKC010000000
VERSION     DASTKC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85132"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1131)
                     /locus_tag="VFR25_04695"
     CDS             complement(61..1131)
                     /locus_tag="VFR25_04695"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HET9940377.1"
                     /translation="MTDPKILDSAEAMARIDRDGMGDRIDAFAAQLRAGAGIAGEVFD
                     RLDPGRPGRVVLFGMGGSAIAGDLARGLVDREGASPLHVCRHYEPPSWIAPEDFLVFS
                     SYSGETEETLEAYRRMRGRGEDALVVSTGGALSRLAAEDGVPVAKLPAGHPPRAALGY
                     SVSTLLHVLGHRGLLTDGSARIEAAAQAVEVVASACGRGVIQSRNSAKQLALRLVDRA
                     LVLIANERTLGPASLRWKGQLNENSKHLAWASPLPEMNHNEVDGYASPAGWVGSVAAV
                     LLRDPGDHPRVVARFRWLERYLAGRGVQVETVEAPDGDAMSRVLGCVAMGDWVSYYLA
                     LLHGSDPSALPGVTSLKQALLK"
     gene            complement(1128..2873)
                     /gene="ptsP"
                     /locus_tag="VFR25_04700"
     CDS             complement(1128..2873)
                     /gene="ptsP"
                     /locus_tag="VFR25_04700"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9940378.1"
                     /translation="MTTLSGIAASPGIGIGPVHVLDPEEIEVKDGLIPAEEVKSEQDR
                     FRAAIEASRREVAELRRKIALETGEEHAHLLDPQLAILEDPEATSQALAAIERDRRSA
                     GYIYRKILGVVAAALEESDGEYIRERALDIRDVRRRVLGHLGGIRSHTLSDLRVPSLV
                     VAYDIAPSEMALASREKILGLAIDLGGRTSHTAIMARARGIPTVVGLKQAMDAAREGA
                     TAVVDGTRGVAIFDPEPEVLEEHRLKQRRYREMAANLASIKDKDCVTQDGVDVHLGAN
                     LEVPEELPSVIENGAEAIGLYRTEFFYLSRSTLPTEDAQYQAYRTVVEAMAPRPVTIR
                     TLDVGGDKFASYLGTPEERNPFLGMRGLRFSLQREDIFRTQLRAICRATVHGRVRVMF
                     PMVIGLEDFRRARQIVLEVRADLEREEVPMATHVPLGVMVETPAAVFAIDLLAREADF
                     VSIGTNDLIQYTLAVDRGNEETAPIYEPLHPAILRALQAVVEAGRKAGIPVGICGEMA
                     GEPLYAVLLLGLGLTEFSVSPFLVPEIKTILNASTRCDAEEFAQRCLALDTPAQVRAL
                     VTEEMSARYGAYFIA"
     gene            complement(2873..3139)
                     /locus_tag="VFR25_04705"
     CDS             complement(2873..3139)
                     /locus_tag="VFR25_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9940379.1"
                     /translation="MVEQNVMIQNRLGLHARACSVFVKEAARFASHIVVARDGLEVNG
                     KSILGVMMLAAERGAEITLRAEGSDEVQALAALVKVVNDKFGEE"
     gene            complement(3147..3998)
                     /locus_tag="VFR25_04710"
     CDS             complement(3147..3998)
                     /locus_tag="VFR25_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HET9940380.1"
                     /translation="MSDLNEVNEVREAPAAIRVRSEDLVRTGARALHIQTLLTPERMQ
                     GPGFAFALLPVLERLYPARNDLARAIRRHLVYFATHPILAGYVLGAAARWEERRANGE
                     PVEAEAIDTWKRALASPLAAMGDPFFWVTLRPLAGLVGIIAIGLIPAPTLGPDWRVLL
                     CPLLTLLTYNAFALPFRVVGTHVGYIEADRPAALLRSLRLREWGHALGCAGALGYGAL
                     LALVIRFLDFGSIDWGLDATAKITTAAPLVVGAVIGWAGLLKWPGRVVEVALAALAAG
                     LLLGGLL"
     gene            complement(3995..4816)
                     /locus_tag="VFR25_04715"
     CDS             complement(3995..4816)
                     /locus_tag="VFR25_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940381.1"
                     /translation="MNLIPEMVRVAILGGVLAVDRAAGWSTMLSQPIVGACLAGALVN
                     PGPEWELWALRVPIGVGAILQLLLTDASLPAAQRQHDTATAGVVGSTVALLGMVRLHS
                     QIPAALGGGLWVLVGVAAGLLSAVAGGWVMGYHRARSRRDVARAEELAGSDSAEGEAA
                     LLDALEGVRAGRRPAAKFELLYWMAMGRVFLVGAFWSWAGSLLGLMLAIAVLPMLGEW
                     LTGRRIGFVFATTLGAALAAGYHAHVRSRRAGGRWMALGAAAALVLLAMARQTNS"
     gene            complement(4813..5313)
                     /locus_tag="VFR25_04720"
     CDS             complement(4813..5313)
                     /locus_tag="VFR25_04720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET9940382.1"
                     /translation="MPLLLARIDDRLIHGQVAYGWGRALKPTFYLVVSDALRADALRA
                     EVCLCGVPDDARGRVVSVAEALTPSVQSEIESERTILLMPGTDEAVRLLEGGFPLTEL
                     NVGGLHHAPGKREVLPYVFLDDADKERLRAVLGRGVRVAARDLPANPSHPIESWLDAP
                     SRGSER"
     gene            complement(5317..5709)
                     /locus_tag="VFR25_04725"
     CDS             complement(5317..5709)
                     /locus_tag="VFR25_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940383.1"
                     /translation="MAGPRPFALLVTHGVLGEELVRTVGAILGPQTDTITISNNGLSS
                     DALTAAIEGRLSEIPPDAPVVLFTDLAAGSCGIASRRVAPSERPFRRITGVNLPMLLE
                     FFHYRDTLSMDELLPRMEAKAKAGILVL"
     gene            complement(5716..>6463)
                     /gene="hprK"
                     /locus_tag="VFR25_04730"
     CDS             complement(5716..>6463)
                     /gene="hprK"
                     /locus_tag="VFR25_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET9940384.1"
                     /translation="RAALDRFFQFQAPCIIVAKGLEIPSELVGRANDHEIPVFRTPQD
                     TTPFIHQLTAFLDFVFAPNTTVHGSLVDVYGIGLLFTGRSGIGKSETALDLVERGHRL
                     VADDVVRITRRHGDILIGACNEVLKHTMEVRGIGIIDVQSIFGIRSIRGQKRVEVEVH
                     LTEWNDARDFERVGLEDQMVSSLDVQIPLVTIPIFPGKNITVIAEVVALNHLVKVYGY
                     HPARALNDRLMESMRMKQLLANPLVRDDVE"
BASE COUNT         1036 a         2262 c         2233 g          932 t
ORIGIN      
        1 tgtgcgtgtg cggccggagc cgcgtgccgg cccctgccac cgggatcacc gcgatcggct
       61 tcacttcaag agcgcctgct taagcgacgt cacgccagga agcgcgctcg gatccgagcc
      121 gtggaggagt gccaagtagt agctcaccca gtcccccatg gccacgcacc cgagcacgcg
      181 gctcatcgca tcgccgtccg gcgcttcgac cgtctccacc tgcacgccgc gtccggccag
      241 gtagcgctcg agccaccgga accgcgcgac gacgcgcgga tggtcccccg gatctcggag
      301 gagcacggcc gcgacgctgc cgacccatcc cgccggcgac gcgtacccgt ccacttcgtt
      361 gtgattcatt tccggaagcg gcgacgccca ggccaggtgc ttcgaattct cgttcagctg
      421 ccctttccaa cgcagcgacg ccggccccag cgtgcgttcg ttcgcgatca ggaccagagc
      481 ccggtcgacc aaacgtaatg ccaattgttt ggcagagttg cgcgattgta tcacacccct
      541 cccgcaggcg gaagcgacca cttccacggc ctgggctgcg gcttcgatcc tggctgagcc
      601 gtcggtcaga aggccccggt ggccgaggac gtggaggagc gtcgagacgg agtagccgag
      661 cgccgcgcgc ggggggtgtc cggcggggag tttggccacg ggcacgccgt cctccgccgc
      721 tagcctcgag agcgcgcccc ccgtcgacac gacgagcgcg tcctcgccgc gtccccgcat
      781 ccggcgatac gcttcgaggg tctcctcggt ctccccggaa taggacgaga agaccaggaa
      841 gtcctccggc gcgatccagg aaggcggctc gtagtggcgg cagacgtgga gcggcgaggc
      901 cccctcgcga tcgacgagcc cgcgcgcgag atcgccggcg atggccgatc cccccatgcc
      961 gaagaggacc acgcgtcccg ggcgccctgg gtcgagccgg tcgaagactt cgccggcgat
     1021 gccggcgccc gcacggagct gcgcggcgaa cgcatcgatc cgatcgccca tgccgtcccg
     1081 gtcgatccgc gccatcgcct ccgcggaatc gaggatcttc gggtccgtca cgcgatgaag
     1141 taggcgccgt accgggccga catctcctcg gtcacgagcg cgcgaacctg ggcgggggtg
     1201 tcgagggcga ggcagcgctg agcgaattcc tcggcgtcgc agcgcgtgga tgcgttcagg
     1261 atggtcttga tctcgggcac gaggaacggg ctcacgctga actcggtcag gccgagcccg
     1321 aggaggagca cggcgtagag cggctccccc gccatctcgc cacagatccc gaccggaatc
     1381 ccggccttcc gcccggcctc gaccacggcc tggagggcgc ggaggatcgc cgggtggagc
     1441 ggctcgtaga tgggcgcggt ctcctcgttg ccgcggtcga ccgcgagcgt gtactggatg
     1501 agatcgttcg ttccgatgct cacgaagtcg gcctcgcgcg cgaggaggtc gatcgcgaag
     1561 acagcagcgg gcgtctccac catcacgccg agcgggacgt gcgtggccat cggcacttcc
     1621 tcccgctcga ggtcggcccg cacctcgagc acgatctgcc gcgcgcgccg gaagtcttcc
     1681 aagccgatca ccatcgggaa catcacgcgc acccggccgt gaacggtcgc gcggcatatc
     1741 gcgcggagct gcgtgcggaa gatgtcctcg cgctggagcg agaagcggag tccgcgcatg
     1801 ccgaggaacg gattccgctc ctcgggcgtt ccgagatacg aggcgaactt gtcgcccccc
     1861 acgtccagcg tgcggatggt cacggggcgc ggcgccatgg cctcgaccac cgtgcggtaa
     1921 gcctgatact gcgcgtcctc ggtcggaagc gtggagcgcg agaggtagaa gaactcggtc
     1981 cggtagaggc cgatcgcctc ggcgccgttc tcgatcacgg acggcagctc ttcgggcacc
     2041 tcgaggttcg cccccagatg gacatcgacg ccatcctgcg tcacgcagtc cttgtccttg
     2101 atcgacgcca ggttcgcggc catttcccgg tagcggcgct gcttcagccg gtgctcctcg
     2161 agcacctccg gctccggatc gaagatcgcg acgccccgcg tgccatcgac gacggccgtg
     2221 gccccttcgc gagctgcgtc catggcctgc ttcaatccga ccacggtggg aatgccgcga
     2281 gcgcgggcca tgatggcggt gtgcgacgtg cgtccgccca ggtcgatcgc gaggcccagg
     2341 atcttctccc gcgaggcgag cgccatctcg ctcggcgcga tgtcgtaggc gaccacgagc
     2401 gacggaacgc ggaggtcgct caacgtgtgc gagcggatgc cgcccagatg gccgagcacg
     2461 cggcggcgca cgtctcgaat gtcgagcgcg cgctcccgga tgtactcgcc gtccgactcc
     2521 tcgagcgcgg cggccacgac gcccaggatc ttgcggtata tgtagccagc cgagcggcgg
     2581 tcgcgctcga tcgcggccag ggcctgcgac gtggcttcgg gatcctcgag aatcgcgagc
     2641 tggggatcga ggaggtgcgc gtgctcctcc ccggtctcga gcgcgatctt cctccggagc
     2701 tcggcgacct cgcggcggct cgcctcgatg gcggcgcgga agcggtcctg ctcggactta
     2761 acctcctcgg ccgggatgag tccgtccttc acctcgatct cttcgggatc gagcacgtgg
     2821 accgggccga tgccgatgcc tggggaggcc gcgatgccgg agagcgtggt catcattcct
     2881 cgccgaactt gtcgttcacg actttcacga gcgccgcgag agcctgcacc tcgtccgagc
     2941 cctcggcgcg cagcgtgatc tcggccccgc gctccgccgc gagcatcatg acgccgagga
     3001 tgctcttccc gttcacctcg agcccgtccc gcgcgacgac gatgtgcgac gcgaaccggg
     3061 ccgcctcctt cacgaacacc gagcaggcgc gcgcgtggag accgagccgg ttctggatca
     3121 tcacgttttg ctcgaccatc gcgctcttag agcagccctc ccagtagaag ccctgcggcg
     3181 agcgccgcga gcgccacttc gaccacgcgt cccggccact tgagaagacc ggcccagcca
     3241 atcaccgccc caacgacgag cggcgccgct gtcgtgatct tggccgtggc gtccagaccc
     3301 caatcgatcg agccgaagtc gaggaatcgg atgacgagcg cgaggagggc cccgtacccg
     3361 agcgcccccg cgcatcccag cgcgtggccc cattccctga ggcggagcga tcgcagaagc
     3421 gccgcgggcc ggtccgcctc gatgtatccg acgtgggtcc cgacgacgcg gaacggcagc
     3481 gcgaaggcat tgtacgtcaa aagggtgaga agcgggcaga ggagcacgcg ccagtccggt
     3541 cccagggtgg gcgcggggat gagaccgatc gcgatgatgc ccacgagacc cgcgagcggg
     3601 cggagcgtga cccagaagaa ggggtccccc atcgcggcga gcgggctcgc cagcgcgcgc
     3661 ttccaggtat cgatcgcctc ggcctccacc ggctcgccgt tggcgcgacg ctcctcccag
     3721 cgcgccgcgg cgccgagcac gtagccggcg aggatcgggt gcgtcgcgaa gtagacgaga
     3781 tgccgccgga tcgcgcgagc cagatcgttc cgcgcgggat acagccgctc gaggacgggc
     3841 aacagtgcga aggcgaagcc cggcccctgc attcgctccg gcgtgagcag ggtctggatg
     3901 tggagcgcgc gcgcgccggt gcgcaccaga tcctcggaac ggacccggat cgcggcggga
     3961 gcctctcgga cctcgttcac ctcgttcaag tcgctcacga gttcgtttgc ctcgccatcg
     4021 cgaggagcac gagcgccgcg gccgcgccga gcgccatcca gcgtccaccg gcccggcggc
     4081 tccgcacgtg cgcgtgatag cccgccgcga gcgccgcacc gagcgtggtc gcgaacacga
     4141 acccgatccg gcggcccgtg agccactcgc cgagcatggg cagcacggcg atcgcgagca
     4201 tgagcccgag caaggacccc gcccatgacc agaacgcgcc gaccaggaac acgcgaccca
     4261 tggccatcca gtagaggagc tcgaacttgg cggccggtct cctcccggcc ctgactcctt
     4321 cgagggcatc gaggagggcg gcctcgccct ccgccgaatc gctgcccgcg agctcctccg
     4381 cgcgagccac gtcgcggcgg ctccgcgcgc ggtgataccc catcacccac cctcccgcga
     4441 ccgcggagag aagcccggcc gccacgccca cgagaaccca gaggccgccg ccgagcgcgg
     4501 ccgggatctg gctgtgcagg cgaaccattc cgaggagcgc gaccgtgctc ccgacgacgc
     4561 ctgcggtggc ggtgtcgtgc tgacgctgcg ccgccgggag cgacgcgtcc gtgaggagga
     4621 gctggaggat ggcgccgacg ccgatcggca cccgcagcgc ccagagctcc cactccggcc
     4681 ccggattcac gagggcgccc gcgaggcaag cgcccacgat cggctggctc agcatcgtgc
     4741 tccagcccgc agcgcggtcc accgcgagca cgccgcccag gatcgcgacg cgcaccatct
     4801 cggggatcag attcaacgct ccgagcctct cgatggagcg tcgagccagg attcgatggg
     4861 atgcgatgga ttggcgggaa gatcgcgcgc ggccacgcgc acgccgcgcc caaggacggc
     4921 gcggagacgc tccttgtccg cgtcgtcgag gaagacgtac ggaaggactt cgcgctttcc
     4981 cggcgcgtga tggaggccgc ccacgttgag ctcggtcagc gggaagccgc cctcgaggag
     5041 ccgcaccgcc tcatcggttc cgggcatgag gaggatcgtc cgctccgact cgatctcgct
     5101 ctggaccgaa ggcgtgagcg cctccgccac ggagacgacc cgcccgcgcg catcgtcggg
     5161 aacgccgcac agacagacct ccgcgcgcag cgcatccgcg cgcagcgcgt cggacacgac
     5221 gaggtagaag gtcggcttga gggcgcggcc ccacccgtag gccacctggc cgtggatgag
     5281 gcgatcgtcg atgcgcgcga ggaggagcgg caccgtctag aggacgagga tgcccgcttt
     5341 ggccttcgct tccattcgcg gcagaagctc gtccatggag agcgtatccc ggtagtggaa
     5401 gaactcgagc agcatgggca gattgactcc cgtgatgcgg cggaaggggc gctccgacgg
     5461 tgcgacgcgc cgtgacgcga tgccgcagct ccccgcggcg agatccgtga agaggacgac
     5521 cggcgcgtcg ggcgggatct cggagagccg gccctcgatc gccgcggtga gggcatcgga
     5581 cgagagcccg ttattgctga tcgtgatggt atcggtctgt ggccccagga tggcgccgac
     5641 ggtccggacg agctcctccc ccagaactcc gtgggtcacg aggagggcga aggggcgcgg
     5701 gccggccatg gcggcctact cgacgtcgtc ccgcacgagc gggttcgcca ggagctgttt
     5761 catgcgcatg ctctccatca atcggtcgtt gagcgcgcgc gcgggatggt agccgtagac
     5821 cttgaccagg tggttgagtg cgaccacttc cgcgatgacg gtgatgttct tccccggaaa
     5881 gatcggaatc gtgacgagcg ggatctggac gtcgagagag ctcaccatct ggtcttcgag
     5941 gcccacccgc tcgaaatcgc gcgcgtcgtt ccactccgtg agatgcacct cgacctccac
     6001 ccgcttctgg ccccggatcg accggatgcc gaagatcgat tggacgtcga tgattccgat
     6061 cccccgcacc tccatcgtgt gcttgagcac ctcgttgcag gcgccgatca ggatgtctcc
     6121 gtgccgccgg gtgatccgga cgacgtcgtc cgccacgagg cggtggccac gctcgaccaa
     6181 gtcgagggcg gtctcgctct tcccgatccc gctccgtccg gtgaagagga ggccgatccc
     6241 atacacgtcg acgagcgacc cgtgcacggt ggtgttcggg gcgaagacga agtcgagaaa
     6301 ggcggtcagc tggtggatga agggcgtcgt gtcctggggc gtgcggaaca ccgggatctc
     6361 gtggtcgttg gcgcgtccca ccagctcgga cgggatctcg aggcccttgg cgacgatgat
     6421 gcagggcgcc tggaactgga agaaccggtc cagggcggca cgc
//
LOCUS       DASTKC010000115         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_321928, whole genome shotgun sequence.
ACCESSION   DASTKC010000115 DASTKC010000000
VERSION     DASTKC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321928"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VFR25_04735"
     CDS             <1..1409
                     /locus_tag="VFR25_04735"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9940385.1"
                     /translation="RLMLHRSNPIHSKRLSSVLAGLALTLATALPAQAQDRVVPNTPA
                     KPRVPIYDPGQPVERKVLKNGVRLIVQEQRTSERVGGVVALRMGTLYENEDESGLSQV
                     LMKSMLAATGSRTPVQMQLELTGADNAKLASGAGSDIGQISIGTTRERASKAVELLAD
                     VVLHPAFPDTAFESAKALYLGKATDELESPLAASYALFLNTMYRGSAFERPAYGLVHS
                     IAAARRSDIVTLYRKLFVGGNVTVAFVGNFDGKKLMAQLEKAFEALPDGAAPARVSGD
                     AAVATTDTLVQEERPILANAVTFGFPAPGYDDPDYPAFMVVDSYLRSGDRSPITYWLP
                     ERQLATGVGVLYPPYPKRSSVAVYYGATSANLPAARDTVLSVMRGLTKNPLDEGEWGV
                     QIRRVQNSFFDDQTNPLVRARNLSRYETEGVGLDYPQRFETRLLQLKPEDVRATAEKW
                     FTHSVEAMLVPTQSGSRP"
     gene            1446..2090
                     /locus_tag="VFR25_04740"
     CDS             1446..2090
                     /locus_tag="VFR25_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9940386.1"
                     /translation="MNDPVGLILLLPPLLFALTFHEAAHGWMALRLGDPTAKLLGRLT
                     LNPLAHLDPLGTLIFIIPPHIGWAKPVPVDVRYLKHPRRDMMWIALAGPVSNVILAFA
                     FGMILRGMNAVSHDFTSPAERALVNMVAWSVVLNLSLAAFNLIPIFPLDGSKVLTGLL
                     SPTAAARFQQLEPVGPILLLGLILIGSFSGVSVIGTIISPFTSTLGRLFTGGIL"
     gene            2087..>2614
                     /gene="trpS"
                     /locus_tag="VFR25_04745"
     CDS             2087..>2614
                     /gene="trpS"
                     /locus_tag="VFR25_04745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9940387.1"
                     /translation="MTAARPRILSGMRPTGKLHLGNYLGALQNWVALQDTSENLHMVA
                     DWHALTTDYEHVDQLEPNTREMVIDWLAAGIDPEKSPVFIQSRVKEHAELHLLFSMLV
                     TTARLERNPTVKEQVRDLNLEETVSYGHLGYPVLQAADILLYKANLVPVGEDQVPHVE
                     LTREIARKFNTLYGEV"
BASE COUNT          427 a          922 c          827 g          438 t
ORIGIN      
        1 gcaggctcat gctccacagg tcgaatccca ttcattccaa acgactttcc tcggttttgg
       61 ctggtctggc cctgaccctg gccacggccc tgccggccca ggcccaggac cgggtggtgc
      121 cgaacacccc ggccaagcct cgggtgccca tctacgatcc ggggcagccg gtcgagcgga
      181 aggttctgaa gaacggggtg cgcctcatcg tccaggagca gcggaccagc gagcgggtcg
      241 gcggggtggt cgccctccgg atggggaccc tgtacgagaa cgaggacgag agcgggctca
      301 gccaggtgct catgaagtcc atgctcgcgg ccaccggcag ccggacgccg gtgcagatgc
      361 agctcgagct gacgggcgca gacaacgcga agctcgcttc gggcgccgga tccgacatcg
      421 gccagatctc catcggcacc acccgggagc gcgcgtcgaa ggcggtcgag ctcctcgccg
      481 acgtggtgct ccacccggcg ttccccgata cggcgttcga atcggccaag gcgctctacc
      541 tcggcaaggc gaccgatgag ctcgagagcc ccctggccgc gtcgtacgcc ctgttcctca
      601 acacgatgta ccgcggtagc gcgttcgagc gtcccgccta tggcctggtg cactcgatcg
      661 cggcggcgcg gcgcagcgac atcgtcacgc tctatcgtaa gctcttcgtc ggtggaaacg
      721 tcaccgtcgc gttcgtcggt aacttcgacg gcaagaagct gatggcgcag ctcgagaagg
      781 cgttcgaggc actgcccgac ggcgcagctc ccgcgcgcgt cagcggcgac gccgcggtgg
      841 cgaccacgga cacgctggtc caggaagagc ggccgatcct cgccaacgcg gtgacgttcg
      901 gcttcccggc tcccggatat gacgaccccg actatcccgc cttcatggtc gtggattcct
      961 acctgcggtc cggcgatcgc tctccgatca cctactggct ccccgagcgg cagctcgcga
     1021 ccggagtcgg cgtcctctat ccgccgtatc ccaagcgctc ctcggtggcg gtctactacg
     1081 gcgccacgtc ggccaacctc ccggcggcgc gcgacacggt cctgtcggtg atgcgcgggc
     1141 tcacgaagaa tccgctcgac gaaggggagt ggggcgtcca gatccgtcgc gtgcagaaca
     1201 gcttcttcga cgatcaaacg aatccgctcg tccgggcgcg caatctgtcc cgctacgaga
     1261 ccgaaggcgt ggggctcgac tatccccagc gcttcgagac ccggctgctc cagctcaaac
     1321 ccgaggacgt gcgcgccacc gccgagaagt ggttcacgca ctccgtcgag gccatgctcg
     1381 taccgacgca gagtgggtcc cgcccctagc gccattcggc gcgtggcggg gcgaggggag
     1441 ggggagtgaa cgatccggtc ggcctgatcc ttctcctgcc gccgctgctc ttcgccctca
     1501 cgttccatga agccgcccac ggatggatgg ctcttcggct gggcgatccc accgcgaaac
     1561 tgctcggccg cctcacgctg aatccgctcg cgcatttgga cccgctcggc acgctcatct
     1621 tcatcatccc gccccacatc ggatgggcca agccggtgcc ggtcgacgtc cgctacttga
     1681 agcacccccg gcgggacatg atgtggatcg cgctcgccgg tccagtctcg aacgtgatcc
     1741 tcgcgttcgc cttcggaatg atcctccggg gaatgaacgc cgtttcgcac gacttcacga
     1801 gccccgccga gcgcgcgctc gtcaacatgg tcgcgtggag cgtcgttctc aacctgagcc
     1861 tcgcggcgtt caacctgatt ccgatcttcc cgctcgacgg ctcgaaggtt ctgaccgggc
     1921 ttctgagtcc gacggcggcg gcgcgcttcc agcagctgga gccggtgggc ccgatcctcc
     1981 ttctcggtct catcctcatc ggctcgttct cgggcgtgag cgtgatcggc acgatcatct
     2041 cgcccttcac gagcacgctg ggacgactct tcacgggagg catcttgtga cggcggcgcg
     2101 cccgcgcatc ctgagcggca tgcggccgac ggggaagctc cacctcggga actacctcgg
     2161 cgcgctccag aattgggtcg cgctccagga cacctccgag aatctccaca tggtcgccga
     2221 ctggcacgcg ctcaccaccg actacgagca cgtcgaccag ctcgagccga acacgcgcga
     2281 gatggtgatc gactggctcg ccgcgggcat cgacccggag aagagcccgg tgttcatcca
     2341 gtcccgcgtg aaggagcatg cggagctcca tctgctcttc tccatgctcg tcaccacggc
     2401 ccgtctcgag cgaaatccca ccgtgaagga acaggtccgg gatctgaacc tcgaggagac
     2461 ggtgagctac ggccatctcg ggtatcccgt tctccaggcg gcggacatcc tcctctacaa
     2521 ggcgaacctc gtcccggtgg gcgaggacca ggtgccgcac gtcgagctga cgcgggagat
     2581 cgcgcgcaag ttcaacaccc tctatggcga ggtc
//
LOCUS       DASTKC010000116        11777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_86133, whole genome shotgun sequence.
ACCESSION   DASTKC010000116 DASTKC010000000
VERSION     DASTKC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86133"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFR25_04750"
     CDS             complement(<1..767)
                     /locus_tag="VFR25_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET9940388.1"
                     /translation="MDQALAVHRLHFAFTVTFHYLFPQLTMGLALLLVVLKTIALRNG
                     DERYNHAARFWGKIFAINFAVGVVTGIPMEFQFGTNWSEFSKASGGVIGQSLAMEGVF
                     SFFLESSFLGLFLFGEKRLGPVGHWLSAVAVFVGSWLSGFFIIVTDAFMQHPVGHEVL
                     PGGVIALKSFWTFLLNPWGLWQYWHNMNGALLTGCFVMAGVGAFYLITRQHEEQARLF
                     VRVGVVVGAIASVIQIFPTGDGQGRMLAKHQPATLAA"
     gene            909..1781
                     /locus_tag="VFR25_04755"
     CDS             909..1781
                     /locus_tag="VFR25_04755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9940389.1"
                     /translation="MDRADVTRAVQRNSIARWIRHLLRGHQGIFLDYPVESRQRYGHG
                     QPAHPELLQLLEQGRARYRDLLERVAGLKDELARIPVLPDPGSMEPAWVNGFLPGLDS
                     AALYGMLALQPPKTYLEVGSGNSTRFARRAIRDHGLSTRIVSIDPHPRVEIDSISDQV
                     IRSPLEAVDLTPILALGPGDILFLDGSHRVFMNSDVTVAFLEILPRLARGVRVQIHDI
                     CLPYDYPLGWEDRYYSEQYVLAAYLLGGAKGATVTLPNAFVSADPELSRILEPIWSRL
                     QTVERHGSSFWMEM"
     gene            complement(1797..2762)
                     /locus_tag="VFR25_04760"
     CDS             complement(1797..2762)
                     /locus_tag="VFR25_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9940390.1"
                     /translation="MTGFRRSHSAAMAAAIALALCASSATARPAQPSGPGHTFKQGGA
                     TIWYEVRGKEGGRPLVMVNGGPGFDHTYVLCSDAWDTLAKKRRVVIYDQRGNGRSGAL
                     AAGQSCTLADQIADLDALRAELKADRIDLLGHSWGGFLVMAYATRHPDRVAHLMIVDS
                     AAPKWTDTEFIFKNIYPEAIDKQARLDFAQMVGDSAAGDESLNEYLKMLFVSDAKRDE
                     FMSRASTYKFSRSVNETLNADLGKYDMWPLLPTLTMPTIVMTGRYDFNVAPSTAWKIH
                     NLIPNSKWEVFEKSGHLPYYEEPEKFVQVVERFCGSPLGTERSSR"
     gene            complement(2781..2993)
                     /locus_tag="VFR25_04765"
     CDS             complement(2781..2993)
                     /locus_tag="VFR25_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940391.1"
                     /translation="MSGLAQEFPGEVNARNVDASEPEARSELNALDIKSHGLVIRADD
                     GRFLWKESGHNVNMDDVRGALHQILD"
     gene            complement(3021..3191)
                     /locus_tag="VFR25_04770"
     CDS             complement(3021..3191)
                     /locus_tag="VFR25_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940392.1"
                     /translation="MPRIPGSLATLTLAAFVAGAGCGGGGKAQQASHEAAPQPPAGKA
                     EHVVVSYYYLPG"
     gene            3284..3811
                     /locus_tag="VFR25_04775"
     CDS             3284..3811
                     /locus_tag="VFR25_04775"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9940393.1"
                     /translation="MTHPNEIDTFRATWEREAQKAIRTMEALPPDQYDFRPDPKGRSI
                     GEMAWHLSEIDGCISYGVVERRFDLQDTLPELVRPKEVKLLAPGYRRVHELAIERLQK
                     VRNEELDERVTFFDGTPMTIRDVLWNVLLHHHIHHRAQLVLLCRMSGGRPPGIYGPNR
                     EEMQEIVEKMKAGKA"
     gene            complement(3813..4613)
                     /locus_tag="VFR25_04780"
     CDS             complement(3813..4613)
                     /locus_tag="VFR25_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9940394.1"
                     /translation="MKQKTGPTAKMFFTEALRLLLESRIPFMVGGGYAMKRHTGIGRA
                     PRDLDVFMTPEDARRALKHFEALGYRTEVTFPHWLGKIRKGRAYVDLIWSSGNGIANV
                     DPSWFEYAVEGSVLGHTVFLSPPEEMIWSKAFVMERERFDGADVLHLILARGPEMDWS
                     RLLERFRPHRLVLLSHLVLFLFVYPSEAERIPTWVWDTLVGALEAERRGSSDAPRVCR
                     GTLLSRSAYLDALARGFRDARLPPEGTMRSKDVATWTRAGREEAEQAQ"
     gene            4660..5415
                     /locus_tag="VFR25_04785"
     CDS             4660..5415
                     /locus_tag="VFR25_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9940395.1"
                     /translation="MTDTARLTTVKVVAVADLHYTPEAEASVVPTLYEVADQGDILLV
                     CGDLTHHGLVEEAVALARAAERVSIPILAVLGNHDFHSGQEEAIKGALRGSRIQVLDG
                     DVAVVRDVGFAGVKGFGSGFGRRVLEPWGEAVMKEFVRVSVDEATKLGSALARLKCEH
                     RIALLHYAPVVGTVDGEPPEIHAFLGSSRLEEPLNRHQVRAAFHGHAHHGRPEGRTST
                     GIPVYNVSLPLLRRTRPDRPPCRVLELQVAPSA"
     gene            5481..6857
                     /locus_tag="VFR25_04790"
     CDS             5481..6857
                     /locus_tag="VFR25_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940396.1"
                     /translation="MSRSILALLIAVSLAAGCAGSAKLSKKSEEKLASGDAWKAWQLA
                     IRALDKEPGNPRAQTAATKAGTSIVQDWQRKIRAYAELDSAKAAAEVLALTEFRGEAA
                     RYATIPVGESWAAEERTLRRAGARIHYNLGVEAENAGRPKKACTEFSEARNYVSDYRD
                     VVKREDRAASDAMTRVAVMPFRAMSDDRSFGAELAQAWQDDLVENLAPPTTQFTRVVS
                     GDAVRRSMTLADLDDVSREQAIRMGRKSGAERVVWGSVGAVKSTTKLHLFQDNVWRRV
                     VERGPNGTEVTRWVEVPIQVVARVRDVKVAVDYEVIDTRSGTSLVHRHLDRSSSARVV
                     WTSDRFDGDPSSYVLVSESFRSAHPDRAKEIETRWSDACGAGTTLVQVLEARRNAGSS
                     GRYARQALPRFAAGAAFVFMEDLPPADDLALAALTQSSRSVRDDLVNMDAVDDVDLGV
                     VVPDAATR"
     gene            6992..7711
                     /locus_tag="VFR25_04795"
     CDS             6992..7711
                     /locus_tag="VFR25_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9940397.1"
                     /translation="MAGFLAQLVDGALGMAYGVTASSLLLSYGVPPAATSATVHAAEC
                     FTTGVSGLSHHWFRNVNGAMFRKLLWPGIVGAVVGAYLLTSIPGDRFRPFIAAYLLVM
                     GAVIILKAFRPFPPTAATTHLGVLGFFGALVDAMGGGGWGPVVASTLIVRGHDVKNTV
                     GTVNAVEFFVTVAASVTFFVTIGVSNWPMILGLALGGVPAAPLAAWACKRVRPRPLMV
                     AVGCLVILLSARTLLKHFGVI"
     gene            7808..8305
                     /locus_tag="VFR25_04800"
     CDS             7808..8305
                     /locus_tag="VFR25_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9940398.1"
                     /translation="MKRTLLALALVLAVPMIAIAQDAAAPAASSTHVVLTPPDIKWGA
                     APAGLPTVATAAVLQGNPGEAGQFTVRLRIPANTKVMPHFHPTDENLTVISGQFLIGM
                     GDTFDQKTMKAMPQGSFGILPATMHHYAMAKTACVVQIHGMGPFGITYVNPNDDPRNA
                     PAASK"
     gene            complement(8259..10142)
                     /locus_tag="VFR25_04805"
     CDS             complement(8259..10142)
                     /locus_tag="VFR25_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940399.1"
                     /translation="MEPRPFRFYLSRTARLILAGVALVLAVVAGFQLWRRLDESYPLV
                     QFERSRAAQEIVSLNLYAARERFGSLLPQGDAQIALREVFLRDVLERSLPIHRTFEDG
                     RYEAVLTQASLDLRNGLALITLRGRARMLGQNASPVEAVLELQTHIDVLEFRPGAGTL
                     RAGLAVTGAHVVRAGTGLAQRFLNPAARFFGGLRIEDWNRDRFSLEIPIRLEEAITFP
                     HLTGDISLPQRRVPLAVSVSAYTVLENRFVLSLALEPDTASGEPHRPHPAEWIPFTDA
                     NRAGMERQARRLYESGRGAAMRDTLLSKAFDLASHDPLWKGLIASDRDIVVVVPRPVL
                     QTLWNRIARTYLQGARLDFDPNIHKHLDETLRVKLLGGKVEAGRIKGSLRVTHLVGHL
                     RVTGDPEIRLLPPDGLEIAGPIQVQEGHGRVDLDLEWDPKALVSVVCRGFEYADTLVG
                     QVQPFTHTLRTTIRFAAADSSVAGRPVVQRDRLSLPVELTDSSFVSMRDALVEQDKLL
                     KCGIAMDPDTVMAKVRRLARTKISFRLPAALFKPFTLPLSLEKEYEAGDVRIAASVRQ
                     PEVVVERDYLRFGFMAVLAVRPAGQAMNEPRRPEPPGLSLHASAAELTSKPPERSAGR
                     RSD"
     gene            10160..11413
                     /locus_tag="VFR25_04810"
     CDS             10160..11413
                     /locus_tag="VFR25_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HET9940400.1"
                     /translation="MTANGRDPIVVIGGGAAGFLAAIFAARGAREAGRRVLLLERTHE
                     GGRKILMSGGGRCNVLPSELDPRRFVTASSPNTMKKILLSWPLREQRRFFEEELGMPL
                     ALEEETGKLFPISNKARDVRDRLRDLASREGADLRFDCLVTSLAPREDGWRIGLAGGG
                     EIRVSRVVIATGGLSVPATGSDGTGLDLVRRLGHTIHDTYPALTPLTARPHRYAALTG
                     VSLPVTLEAPLERRSFVTHGGFLFTHKGYSGPAVLNASHLAILSRRAGGPAQRFTVRW
                     TPLDADGWDQELRSGEGTVLARLRMRIPARLAEALLEDARVDGATPLAQLKKDERHRV
                     IEALTRHPLPWSGDEGYVKAEVTGGGVALDEVDPRTLESRVAAGLFLCGEILDAFGPI
                     GGYNFAWAWATGRAAGLGAATAHVS"
     gene            11423..>11777
                     /locus_tag="VFR25_04815"
     CDS             11423..>11777
                     /locus_tag="VFR25_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9940401.1"
                     /translation="MRFALLLVFLVGCATPSYVDAEAPRSPTPKSIDAPVRVETVAEG
                     LEHPWGLEFLPDGRMIVTERPGRLRIVTPDGRLSEPLSGVPRVFAQGQGGLLDVALSP
                     SFERDRLIYLSFSEPE"
BASE COUNT         1880 a         3978 c         4035 g         1884 t
ORIGIN      
        1 atcgcggcga gcgttgccgg ctggtgcttc gccagcatcc ttccctggcc gtcgccggtg
       61 gggaagatct ggatcaccga cgcgatcgcc ccgacgacca caccgacgcg cacgaagagc
      121 cgggcctgct cctcgtgctg gcgcgtgatg agatagaagg cgcccacccc cgccatgacg
      181 aagcagccgg tcaggagcgc gccgttcatg ttgtgccagt actgccagag tccccaggga
      241 ttgaggagga acgtccagaa gctcttcagc gcgatcacgc cgcccgggag cacctcgtgc
      301 ccgacgggat gctgcatgaa cgcgtcggtc acgatgatga agaagcccga cagccaggaa
      361 cccacgaaga cggccaccgc gctcaaccag tggccgaccg gtccgagccg cttctcgccg
      421 aacaggaaga ggccgaggaa cgaggactcg aggaagaagg agaagacccc ctccatcgcg
      481 agcgactgcc cgatcacgcc gcccgacgcc ttcgagaact cggaccagtt ggtcccgaac
      541 tggaattcca tcggaatgcc ggtcacgacg cccaccgcga agttgatcgc gaagatcttc
      601 ccccagaagc gcgcggcgtg gttgtagcgc tcgtctccgt ttcggagcgc gatcgtcttc
      661 aggaccacga ggaggagcgc gagccccatc gtgagctggg ggaagagata gtggaacgtc
      721 accgtgaacg cgaagtggag ccggtggacc gcgagcgctt gatccatggg tgtctcctca
      781 acgggacgaa ctgggctcga atggggggag catagcggcg ggcgttcagg gctgccatgc
      841 tccttccgga tgacgcgccg cgcgcgcggg cttcgcgcct gctcggccca gtgctagatt
      901 cgcggcccat ggaccgcgcc gacgtcaccc gagccgttca gcggaacagc atcgcgcgct
      961 ggatccgcca tctcctccgc ggacatcagg ggatcttcct cgactacccg gtagagtccc
     1021 ggcagcgcta cgggcacgga cagccggcgc acccggagct tctccagcta ctggagcaag
     1081 ggcgggcgcg ttaccgggat ttgctcgagc gcgtggcagg cttgaaggac gagctggcaa
     1141 ggatcccggt gctcccggac ccaggctcca tggagccggc gtgggtgaac ggattcctgc
     1201 cggggctcga ttcggctgcg ctctacggga tgctcgcgct ccagccgccg aaaacatacc
     1261 tggaggtggg atcggggaac tcgacccggt tcgcgcggcg cgccatccgc gatcatggcc
     1321 tgagcacgcg gatcgtctcg atcgatccac atcccagggt cgagatcgat tcgatctcgg
     1381 atcaggtcat ccgctcgccg ctcgaagccg tcgatctcac gcctatcctc gcgctcgggc
     1441 cgggcgacat cctcttcctc gacggatcgc accgcgtgtt catgaattca gacgtgacgg
     1501 tcgcgttcct cgagatcctg ccgcggctcg cgcgcggggt gcgcgtgcag atccacgata
     1561 tctgcctgcc gtacgactac ccgctcggat gggaagaccg ctactactcg gagcagtatg
     1621 ttctggcggc gtacttgctg ggtggcgcca agggtgccac ggtcacgctg ccgaacgcat
     1681 tcgtgagcgc ggatccggag ctgtcgcgga tcctcgagcc gatctggagc cggctccaga
     1741 ccgtcgagcg acacggcagc tcgttctgga tggagatgta ggcgcgccgc gcgaggctac
     1801 cgcgagcttc gctccgtccc cagcggcgat ccgcagaacc gctccaccac ctgcacaaac
     1861 ttctctggct cctcgtagta gggcaggtgg ccgctcttct cgaacacctc ccacttcgag
     1921 ttcgggatca ggttgtggat cttccacgcg gtgctcggcg cgacgttgaa gtcgtagcgg
     1981 ccggtcatca cgatcgtggg catggtcaag gtagggagga gcggccacat gtcgtacttg
     2041 ccgaggtcgg cattcagggt ttcgttcacc gaccgcgaga atttgtacgt gctcgccctc
     2101 gacatgaact cgtcccgctt ggcgtccgaa acgaagagca tcttcaggta ttcgttcagg
     2161 ctctcgtcgc ccgccgcgct gtcgcctacc atctgcgcga agtcgagccg ggcctgcttg
     2221 tcgatcgcct cggggtagat gttcttgaag atgaactcgg tgtccgtcca cttgggcgcc
     2281 gcggagtcga cgatcatgag atgcgcgacg cgatcgggat gccgcgtcgc gtacgccatc
     2341 acgaggaagc cgccccagga gtgccccagg agatcgatgc gatccgcctt gagctccgcg
     2401 cggagggcgt ccaggtcggc gatctgatcc gccagcgtgc aggattgacc cgccgcgagc
     2461 gcaccggagc ggccgtttcc gcgctggtcg tagatgacga cgcgccgctt cttcgcgagc
     2521 gtatcccacg cgtccgagca cagcacatag gtgtgatcga accccggtcc gccgttcacc
     2581 atgacgaggg gccgtccgcc ctccttcccg cgcacctcgt accagatggt cgcaccgccc
     2641 tgcttgaacg tgtggccagg tcccgagggt tgagcgggac gcgccgtagc ggacgaggcg
     2701 cacagggcga gagcgatcgc ggcggccatg gccgcggagt gcgaacgacg gaaaccggtc
     2761 acgtcacacc tccaagggta tcagtcgaga atctggtgaa gcgccccgcg aacgtcgtcc
     2821 atgttgacgt tgtggcccga ttccttccag aggaagcgtc cgtcgtcggc gcggatgacc
     2881 aggccgtgcg acttgatgtc gagcgcgttg agctcgctcc gggcttcggg ctccgaagcg
     2941 tcgacgtttc gcgcgttgac ctcgcccggg aactcctgcg cgagcccgct cacggcgggc
     3001 ttcagcttcg cgcagaccgc tcagccaggc aggtagtagt aggaaacgac gacgtgctcg
     3061 gccttgccgg ccggaggctg gggcgctgct tcatgcgacg cctgctgcgc cttcccacct
     3121 ccgccgcagc cagcccccgc cacgaaggcg gcgagcgtga gcgttgcgag ggatcctgga
     3181 atccggggca tggagaacct cctggatagg cccatccgcg tggcgtaggc tcgacgagcc
     3241 gaacgcctcg aagttccgtc cacgatacca ccggaggtcc gtcatgaccc acccgaacga
     3301 aatcgacacg tttcgcgcca cctgggaacg cgaggcccag aaggcgatcc gcacgatgga
     3361 ggcgctgccg cccgaccagt acgacttccg tcccgatccg aagggacggt cgatcggaga
     3421 gatggcctgg catctgagcg agatcgacgg atgcatcagc tacggcgtcg tggagcggcg
     3481 cttcgacctg caggacaccc tgccggagct cgtacgtccg aaggaggtca agctcctcgc
     3541 gcccggctat cgccgcgtgc acgagctcgc catcgagcgg ctgcagaagg tgcggaacga
     3601 ggagctcgac gagcgcgtca cgttcttcga cggcactccg atgacgatcc gggacgtgct
     3661 ctggaatgtg ctccttcacc accacatcca tcaccgcgcc cagctcgtgc ttctctgtcg
     3721 gatgtcaggt ggcaggccgc ccggaatcta cgggccgaat cgcgaggaga tgcaggagat
     3781 cgtcgagaag atgaaggcgg ggaaggcctg acctactgcg cttgctcggc ttcctcgcgc
     3841 ccggcgcgcg tccatgtggc cacgtccttg gatcgcatcg tgccctcggg tgggaggcga
     3901 gcgtcgcgga atccgcgcgc gagcgcgtcc aggtaggcgc tcctcgagag gagcgttccg
     3961 cggcagacgc gcggcgcatc ggaggatccg cggcgctccg cctcgagcgc gcctacgagc
     4021 gtgtcccaca cccaggtggg gatgcgctcc gcctcggagg gatagacgaa gaggaacagc
     4081 acgaggtggc tcaacaggac gagccggtgc ggccggaatc gctccagcag tcgcgaccag
     4141 tccatctccg gcccgcgcgc gagtatgaga tggagcacgt ccgctccgtc gaagcgctcg
     4201 cgttccatca cgaacgcctt cgaccagatc atctcctcgg gcgggctcaa gaagacggtg
     4261 tgtcccagca cggagccctc gacggcgtac tcgaaccagg acggatcgac gttcgcgatt
     4321 ccattccctg agctccagat caaatccacg tacgcgcgtc ccttgcgaat cttgccgagc
     4381 cagtgcggga acgtgacctc ggtgcgatag cccagggcct cgaagtgctt gagcgcgcgc
     4441 cgcgcgtcct cgggcgtcat gaagacgtcc aggtcgcgcg gtgcgcgccc gatccccgtg
     4501 tgccgcttca tcgcatatcc gccccccacc atgaacggaa tgcgcgattc gaggaggagc
     4561 cggagggcct ccgtgaagaa catcttggcc gtcgggcccg ttttctgctt caccgtcata
     4621 cacatcccct agctccgtta aacccggagg ctccgccaga tgactgacac cgctaggctt
     4681 acgaccgtca aggtcgtcgc cgtcgccgat ctgcactaca cacccgaggc tgaggcatcg
     4741 gtcgtgccaa cgctgtacga ggtggcggac cagggggaca tcctgctcgt gtgcggcgac
     4801 ctgacccacc acggtctcgt ggaggaagcg gtggccctcg cgcgcgcggc ggagcgcgtt
     4861 tcgattccca tcctggccgt cctcggcaat cacgacttcc attccggcca ggaggaggcg
     4921 atcaagggcg cgctccgcgg ctctcggatc caggtcctcg acggcgacgt ggccgtggtt
     4981 cgggacgtcg gcttcgcggg ggtgaagggg ttcgggagcg gattcgggcg ccgcgtgctc
     5041 gagccctggg gcgaggccgt gatgaaggaa ttcgtgcgcg tctccgtgga cgaagccacc
     5101 aagctcgggt cagcgctggc gcgcctcaag tgtgagcaca ggatcgcgct cctccactac
     5161 gctccggtcg tcggcacggt ggacggggag ccaccggaga tccatgcctt cctggggagc
     5221 agccgcctcg aggagccgct caatcgccat caagttcgcg ccgccttcca cggccatgcc
     5281 caccacggcc gtcccgaagg ccggacgtcg accggaatcc cggtctacaa cgtctccctg
     5341 ccgcttcttc ggcgcacccg gccggaccgc cctccctgcc gggtcctcga gctccaggtg
     5401 gccccctcgg cctgacccgc cgcttcagtc gagaacgccc ctccccctgc cgataaggag
     5461 tcgcgaaagg gggccctccc atgtctcgat cgatactcgc attgttgatc gccgtatccc
     5521 tggcggccgg atgcgccggc tccgccaagc tgtcgaagaa gagtgaagag aagctcgcgt
     5581 ccggcgacgc gtggaaggcg tggcagctcg ccatccgcgc gctcgacaag gagccgggca
     5641 acccgcgggc gcagaccgcc gcgaccaagg ccggcacctc catcgtccag gattggcagc
     5701 ggaagatccg cgcgtacgcc gagctggaca gcgcgaaggc ggcggcggag gttctcgcgc
     5761 tgaccgagtt ccgcggtgag gcagcgcggt acgccacgat cccggtgggc gagagctggg
     5821 ccgccgagga gcgcacgctc cggcgtgccg gcgcgcgcat ccactacaac ctcggcgtcg
     5881 aggccgagaa cgccggccgt ccgaagaagg cgtgcacgga gttctccgag gcgcgcaact
     5941 acgtgagcga ctaccgcgac gtcgtgaaac gcgaagaccg cgcggcgagc gacgccatga
     6001 cgcgcgtggc cgtgatgccg ttccgggcga tgtcggacga tcgctcgttc ggagccgagc
     6061 tggcccaggc gtggcaggac gatctcgtcg agaacctggc gccgcccacg acgcagttca
     6121 cccgcgtcgt ctcgggggac gcggtgcgcc gctcgatgac cctcgccgat ctcgacgacg
     6181 tgtcgcgcga gcaggcgatc cgtatgggga ggaagtccgg cgccgagcgc gtggtgtggg
     6241 gatcggtcgg ggccgtgaag tcgaccacga agctccatct cttccaggac aacgtctggc
     6301 gtcgcgtcgt ggagcgcggt ccgaacggga ccgaggtgac ccgctgggtc gaggtgccga
     6361 tccaggtcgt cgctcgcgtg cgcgacgtga aggtcgcggt ggactacgag gtgatcgaca
     6421 cgcggagcgg aacgtccctc gtgcaccggc atctcgatcg ctcgagctcg gcgcgcgtgg
     6481 tttggacttc cgaccgcttc gacggcgatc cgtcgagcta cgtgctggtg tcggaatcct
     6541 tccgctcggc gcatcccgat cgcgcgaagg agatcgagac ccgctggagc gacgcctgcg
     6601 gcgccggcac cacgctggtg caggtgctcg aagcgcgtag gaacgctggc tcgtcgggcc
     6661 gctacgcccg ccaggctctg cctcgattcg ccgcgggagc agcgttcgtc ttcatggaag
     6721 atctcccgcc ggccgacgat ctggctctcg cggcgctcac gcagagctcg cgctcggtgc
     6781 gcgacgatct cgtgaacatg gacgcggtgg acgacgtgga tctcggcgtg gtggtgccgg
     6841 acgcggcgac gcgctgacct aggaccaggg ccccttcgaa cgggcgcggc ggccggtgac
     6901 ccggcacgcc gcgcccgtta tcatgtccag gccgtagcca tccccacccg accgcgaggc
     6961 cctactgcca cccgaattcc tgctgtacgt cctggccgga ttcctggccc agctcgtcga
     7021 cggcgcgctg gggatggcct acggcgtcac cgcctcgagc ctcctcctct cgtacggcgt
     7081 tcctcctgcc gcgacgagcg cgaccgtgca cgcggccgag tgcttcacga ccggcgtgtc
     7141 ggggctgtcc caccactggt tccggaacgt gaacggcgcc atgttccgaa agctcctctg
     7201 gcccgggatc gtgggagccg tcgtgggcgc gtatctcctc acgtccatcc ccggcgatcg
     7261 ctttcgcccg ttcatcgccg cctacctgct cgtcatgggg gccgtgatca ttctcaaggc
     7321 gttccggccg ttccccccga ctgccgcgac cacgcacttg ggcgtgctcg gcttcttcgg
     7381 ggctctcgtc gatgcgatgg gaggcggcgg ctggggcccg gtggtcgcga gcacgctcat
     7441 cgtgcggggc cacgacgtga agaacacggt gggcaccgtg aacgccgtgg agttcttcgt
     7501 caccgtggcg gcgtcggtga ccttcttcgt cacgatcggg gtctcgaact ggccgatgat
     7561 cctcggtctg gccctgggcg gggtcccggc ggcgccgctc gccgcctggg cctgcaagcg
     7621 ggtgcggcca cggccgttga tggtggctgt ggggtgcctc gtcatcctcc tgagcgcccg
     7681 caccctcctc aagcatttcg gggtcatcta aggggtcaca acggggcctg gctggagtag
     7741 gataggtggc ccttgccgtc gccgacgggg gtccggcgtc gaaacccaca ccgaggaggc
     7801 acgacagatg aagcgtacct tgcttgccct ggcactcgtt ctcgcagttc ccatgatcgc
     7861 gatcgcgcag gatgcggcgg cgccagccgc gtcgagcacg catgtcgtgc tcacgccgcc
     7921 cgacatcaag tggggcgccg ctcccgcggg tctcccgacc gtggcgaccg cggccgtgct
     7981 ccagggcaat ccgggagagg ccggacagtt caccgtgcgc ctgcgcattc cggcgaacac
     8041 gaaggtcatg ccgcacttcc atccgaccga cgagaacttg acggtgatct cgggtcagtt
     8101 cctgatcggg atgggcgaca cgttcgacca gaagacgatg aaggcgatgc cgcagggaag
     8161 cttcgggatc cttcccgcca ccatgcacca ctacgccatg gcgaagaccg cgtgcgtggt
     8221 tcagatccac ggcatgggac cgttcgggat cacgtacgtc aatccgaacg acgacccgcg
     8281 gaacgctccg gcggcttcga agtaagctca gcagcactcg catgaagaga cagccccggc
     8341 ggctccggcc gccggggctc gttcattgcc tggcccgcgg gacgcaccgc gagcaccgcc
     8401 atgaagccga agcgaagata atcgcgctcc accacgacct cgggctgtcg cacggacgcc
     8461 gcgatccgca cgtccccagc ctcgtactcc ttctccagcg agaggggaag cgtgaacggc
     8521 ttgaagagcg ccgctgggag ccggaaggag atcttggtgc gcgccaggcg ccggaccttc
     8581 gccatgacgg tgtccgggtc catggcgatg ccgcacttca agagcttgtc ctgctcgacc
     8641 aacgcgtcgc gcatgctgac gaacgaggaa tcggtgagct ccaccggcag cgacaggcgg
     8701 tcgcgctgca ccacgggacg gcccgcgacg ctcgagtcgg ccgccgcgaa gcgaatggtg
     8761 gtgcggagcg tgtgcgtgaa cggttggacc tggcccacca gcgtgtcggc gtactcgaag
     8821 ccgcggcaga ccacgctcac gagagccttc gggtcccatt ccaaatcgag atccacccgt
     8881 ccgtgtccct cctgcacttg aatcggcccg gcgatctcga gaccgtcggg cggaaggagt
     8941 ctgatctcgg gatcgcccgt cacgcgaaga tgcccgacga gatgggtgac tcgaaggctc
     9001 cctttgatgc gtcccgcctc caccttgccc ccgagcagct tgacgcggag ggtttcgtcc
     9061 aggtgcttgt ggatgttggg atcgaagtcg agccgcgctc cctggaggta cgtgcgagcg
     9121 attcgattcc agagcgtctg gaggacgggg cggggcacga ccacgacaat gtcccggtcg
     9181 gaggcgatga gacccttcca gagcggatcg tgcgacgcga gatcgaacgc cttcgagagg
     9241 agggtgtctc gcatcgcggc gccgcgtccg ctctcgtaga gccggcgcgc ctggcgctcc
     9301 atgccggccc ggttcgcatc ggtgaacggg atccactcgg cggggtgcgg gcgatgcggc
     9361 tcgcccgacg cggtgtcggg ctccagggcg aggctcagca cgaagcggtt ctcgagcacg
     9421 gtgtaggccg atacgctcac ggcgagggga acccggcgct gcggcagcga gatgtcgccg
     9481 gtcagatgcg ggaatgtgat cgcttcctcc aggcggatcg ggatctccag gctgaagcgg
     9541 tcgcgattcc agtcctcgat ccgaaggccg ccgaagaagc gcgccgccgg gttgagaaag
     9601 cgctgcgcca ggccggttcc cgcccggacc acgtgggctc ccgtgacggc gagcccggcc
     9661 cggagcgtgc ccgcgccggg tcggaactcc aggacgtcga tgtgggtctg gagctcgagc
     9721 accgcttcga ccggcgatgc gttctggccc agcatccgcg cgcggccacg gagcgtgatg
     9781 agcgcgagcc cgttcctgag gtcgaggctc gcctgcgtga ggaccgcctc gtagcgcccg
     9841 tcctcgaacg tgcggtggat ggggagcgac cgttcgagga cgtcgcgcag gaagacctcg
     9901 cgaagcgcga tctgggcgtc cccctgcgga aggagcgagc cgaagcgctc gcgcgcggca
     9961 tagaggttca agctcacgat ctcctgcgcc gcgcggctgc gctcgaactg gacgagggga
    10021 taggactcgt cgagccgccg ccagagctgg aacccggcca ccaccgcgag cacgagcgcg
    10081 acccccgcca ggatcaggcg ggcggtgcgg ctcagataga agcggaacgg gcgcggctcc
    10141 atccctcaag cgtacgccta tgaccgccaa cggacgggat ccgatcgtcg tgatcggcgg
    10201 gggagcggcg ggatttctcg ccgcgatctt cgcggcgcgc ggcgcgcggg aggcgggacg
    10261 ccgcgttctc ctcctcgagc ggacgcacga aggcgggcgg aagatcctca tgtccggggg
    10321 aggccggtgc aacgtgctcc cgtcggagct cgatccgcgg cgattcgtga cggcctcctc
    10381 gcccaacacg atgaagaaga tcctcctgtc gtggccgctt cgcgagcagc gccgcttctt
    10441 cgaagaggag ctcgggatgc cgctcgcgct cgaggaggag acggggaagc tcttcccgat
    10501 ctccaacaaa gcgcgcgacg tgcgcgaccg cctgcgcgat ctcgcgtcgc gcgagggggc
    10561 ggatctccgt ttcgattgtc tggtgacgtc gctcgcgccg cgcgaggacg ggtggaggat
    10621 cgggctcgcc ggcggtggcg agatccgcgt gtctcgagtc gtgatcgcga ccggcggtct
    10681 ctcggtcccg gcgaccggga gcgacggcac gggactcgat ctggtgcgcc gtctcggcca
    10741 cacgatccac gacacctacc ccgcgctcac gcctctcacc gcgcggccgc atcgctacgc
    10801 ggcgctcacc ggcgtgtccc ttccggtcac cctcgaggcg ccgctcgagc gacgctcctt
    10861 cgtgacgcac ggaggattcc tcttcaccca caagggctac agcgggcccg cggtcctgaa
    10921 cgcgtcccat ctcgcgatcc tctcgcggcg cgcgggaggg ccggcgcagc gcttcaccgt
    10981 gcgctggacg ccgcttgacg ccgatggatg ggatcaggag cttcgctccg gggagggcac
    11041 cgtgctcgcg aggcttcgca tgcggatccc cgcgcgactc gccgaggcgc tcctcgagga
    11101 cgcgcgcgtg gacggcgcga cgccgctcgc gcagctcaag aaggacgagc gccaccgtgt
    11161 gatcgaggcg ctcacgcgcc atccgctccc gtggtcgggg gacgagggat acgtgaaggc
    11221 cgaggtcacc ggcggtggcg tggcgctgga cgaggtcgat ccccgcacgc tcgagagccg
    11281 cgtcgccgcc gggctcttcc tctgtggcga gatcctggac gcgttcgggc cgatcggcgg
    11341 ctacaacttt gcgtgggcgt gggccacggg tcgcgccgcg gggcttggag cggcgaccgc
    11401 ccacgtgtcg tagactgagg cgatgagatt cgcgctgttg ctggtcttcc tcgtgggctg
    11461 cgccacgccc tcctatgtcg acgcggaggc gccgcgctct ccgacgccaa agtccatcga
    11521 cgcgccggtg cgcgtcgaga ctgtggccga gggtctcgag catccctggg gcctcgagtt
    11581 ccttcccgac ggacgcatga tcgtgacgga gcgcccgggc aggctccgca tcgtcacacc
    11641 ggacggccgc ctctccgagc cgctctcggg cgtgccgcgt gtgttcgctc aaggccaggg
    11701 cggtctcctc gatgtggcgc tgagtccctc gttcgagcgg gaccgtctga tctatctttc
    11761 gttctcggag cccgagg
//
LOCUS       DASTKC010000117        10652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1978029, whole genome shotgun sequence.
ACCESSION   DASTKC010000117 DASTKC010000000
VERSION     DASTKC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978029"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1089)
                     /locus_tag="VFR25_04820"
     CDS             complement(169..1089)
                     /locus_tag="VFR25_04820"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9940402.1"
                     /translation="MTSSTGAGASAAPIEPPRVAAATRDASLPARLLSAALFLPLLVL
                     LAWIGGVPYLLFTMAVVGLGLREFYLLLEAKGLSPHWKSGMLAVLLLPIGVYLRFRTH
                     RVEEWHVGGFFTILVGAVLLAELRRGAGKQAVANSAATLLGFLYIGWLGTHLGALREL
                     PFPTRRPYHEGMSYALLPFMLVWSCDTAAYVVGRAFGRTKLMPSVSPGKSLEGAIAGL
                     LASIGAAFLARIWFAHYLTALDAVVLGALVGVFGQLGDLVESLLKRDAELKDTSRLIP
                     GHGGVLDRFDSLLFAAPIVYYYLLFQVVTP"
     gene            complement(1046..1792)
                     /locus_tag="VFR25_04825"
     CDS             complement(1046..1792)
                     /locus_tag="VFR25_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET9940403.1"
                     /translation="MKPSSHAVTLEQVLRRPVPGHIAIIMDGNGRWAKRRGLPRLMGH
                     RAGRRSVREVVEGCVELGVKVLTLYTFSLENWERTKGEVKGLMRILEQVLIEERDELK
                     KNNVRLRTIGRVDLLPEHSRQILEETRTFLDSSTGMTLVLALSYGGRAEIVDAARRIV
                     EADRERRIPLASIDEEFFRHRLDTDGLPDPDLLIRTSGELRISNFLLWQAAYSEFWVT
                     DTLWPDFRRKHLYQAVYDFQHRSRRFGRAD"
     gene            complement(1915..2472)
                     /gene="frr"
                     /locus_tag="VFR25_04830"
     CDS             complement(1915..2472)
                     /gene="frr"
                     /locus_tag="VFR25_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9940404.1"
                     /translation="MDPNFVKHAEERMKKAVETIRHEVASIRTSKATTSLLDSIRVEY
                     YGNPVPLQQVASVSAPEARLLVVQPWERNIVPEVVKAIQKSDLGLNPVDDGTVIRLAL
                     PPLTEERRKDLVKVLGKIVEDGRVRVRTVRRESNDELKKLEREHKISEDDGKKTHDQV
                     QKVTDRYIAVLDEIFHKKQAEVMEV"
     gene            complement(2476..3207)
                     /gene="pyrH"
                     /locus_tag="VFR25_04835"
     CDS             complement(2476..3207)
                     /gene="pyrH"
                     /locus_tag="VFR25_04835"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9940405.1"
                     /translation="MPVEPARRVLVKLSGEILAGDQGMGIDPDALGRVADEIKEGHDG
                     GAEIGIVIGGGNLMRGATAAAAHGDQIDRTTADSMGMLATVMNALALQDALERRGIAT
                     RVLSAIEMRTLAELYIRRRAVRHLEKGRVVILAAGTGHPYFTTDTAAVLRAVEIGADV
                     LLKATKVDGVFTEDPRRNPAARRFDVLQYKEAIDRRLSVMDQTAFTMGMENHLPLLVF
                     NVTVPGNLARALKGEAVGTRVAVEV"
     gene            complement(3207..3803)
                     /gene="tsf"
                     /locus_tag="VFR25_04840"
     CDS             complement(3207..3803)
                     /gene="tsf"
                     /locus_tag="VFR25_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9940406.1"
                     /translation="MTITASLVKELRERTGAGMMECKKALSEVAGDLEKAIDVLRKSG
                     MARASSKAGREARQGLIHAYIHPGDQLGVLLEVDCETDFVARTDDFRDLVRNIAMQVA
                     ATNAQAVDRNSIDPGMIERERAILMEQALTTGKPEAVLQKIVDGKIEKYYEEICLLEQ
                     PYIKDPDRKVKELVDQAIAKMGENIVVRRFSKFRIGDA"
     gene            complement(3849..4610)
                     /gene="rpsB"
                     /locus_tag="VFR25_04845"
     CDS             complement(3849..4610)
                     /gene="rpsB"
                     /locus_tag="VFR25_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9940407.1"
                     /translation="MADIGMKELLEAGVHFGHQTRRWNPKMKKFIFIERNGIYIIDLQ
                     KTLKALHDARQLLEGIGKKSGSVLFVGTKRQAKDAILEEAGRCTMPYVNERWLGGMLT
                     NFKTIKSNIRRFKEIEKMDADGTLEKLSKKEQAQINKERTRLVKIFTGIKEMAQLPSA
                     VFVIDTKKERIAVAEANRLGIPVIGVVDTNCDPDVVDYPLPGNDDAIRAIRVFARFVS
                     DTLMSARQEAQEGADMAAELAAKEAPAEPSAVANA"
     gene            complement(4747..5136)
                     /gene="rpsI"
                     /locus_tag="VFR25_04850"
     CDS             complement(4747..5136)
                     /gene="rpsI"
                     /locus_tag="VFR25_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005377846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9940408.1"
                     /translation="MANHVQGTGRRKTSVARVRLVPGTGKRLVNGVDLDRYFSRITLS
                     RQAIGPLVAGNLVEKYDIHATVHGGGHAGQAGAISLGVARALQTADPSLRGLLGKEGF
                     LTRDARMKERKKYGQAGARKRFQFSKR"
     gene            complement(5139..5594)
                     /gene="rplM"
                     /locus_tag="VFR25_04855"
     CDS             complement(5139..5594)
                     /gene="rplM"
                     /locus_tag="VFR25_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9940409.1"
                     /translation="MGTYSVRAAEIVQDWYVADAEGQILGRLASDIASVLKGKRKPTY
                     STHLDVGDHVVVINAEKVRVTGRKRDDKTYFRHSLYPGGHTLTRFRDLQAQKPDEIIR
                     LAVKGMLPKNRLGRAMIKKLKVYAGSDHPHTAQQPRPFPWLDGRLKGGE"
     gene            complement(5637..6683)
                     /gene="mtnA"
                     /locus_tag="VFR25_04860"
     CDS             complement(5637..6683)
                     /gene="mtnA"
                     /locus_tag="VFR25_04860"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET9940410.1"
                     /translation="MSRDPFRFEGDRVLLLDQTLLPDREVMIEIRDARAMADAISRLA
                     VRGAPAIGIAAALALAVEATREGSASKQLEWLEDAGRLLVASRPTAVNLAWAVDRVLG
                     AARRAVAKDSENLTARVRDEALAIWDEDRAASRAMAEHGASLFPTQTRFLTHCNTGAL
                     ATGGGGTALAVLLELHRRRPNGIEVWATETRPLLQGARLTAWELREAGVPFRLITDSA
                     AAHTILRGGIEGVLVGADRIARNGDSANKIGTLGLALAAHAAGIPFVVVAPTSTIDPR
                     IPDGAEIAVEARSAEEVTAIRGMRLAPTGIRAENPAFDVTPARWIGAIVTERGVQRGP
                     AYDLGGVVDTKTRP"
     gene            complement(6680..7306)
                     /locus_tag="VFR25_04865"
     CDS             complement(6680..7306)
                     /locus_tag="VFR25_04865"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET9940411.1"
                     /translation="MQTNLINPLGPKLILASRSPRRVELLTLVGARFTTFPVDVDESA
                     LPGERPDAHVQRLAEAKARAAAEARGGNGADDEAVVVGADTIVSLDRAILGKPSGPEE
                     AARMLERLSGRIHEVWTGLFLLDPVAGRAVGGALRSIVKFAPLDADDVARYVATGEPL
                     DKAGAYAVQGKGAVFVEAIEGSYSNVVGLPLSYLKHLLGLLVERAGRV"
     gene            complement(7311..7754)
                     /gene="dtd"
                     /locus_tag="VFR25_04870"
     CDS             complement(7311..7754)
                     /gene="dtd"
                     /locus_tag="VFR25_04870"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011748037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET9940412.1"
                     /translation="MRALVQRVSRAAVRVDGAEISRIGPGLLILLGVRAGDPEGAEER
                     LAERCAELRIFEDEAGKMNRSVRDLGGEALVVPQFTLYADTSRGRRPGFEAAAAPTLA
                     EPAWRRFCDALAARGVPTQGGRFGASMEVELVNRGPATFLLETTD"
     gene            complement(7751..9955)
                     /locus_tag="VFR25_04875"
     CDS             complement(7751..9955)
                     /locus_tag="VFR25_04875"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET9940413.1"
                     /translation="MVPVPADLPGPLLEALQRSNRALDLEKIAAAYLFVVDAHGSQVR
                     KSGEPYVTHLASVATILADLLGSGVDEVLIQAALLHDVVEDTPITLPQLERRFGRDVA
                     ALVDGVTKIGHLHFERPEQEEAENFRKMLFSMARDLRVILIKLADRLHNMRTLQALGP
                     ARAHRIARETREIYAPLAHRLGIARIKWELEDLCLKHLDPDAYREIRDKVQLKREERE
                     RQIQDVIQPVKSRLVHLGMKADVVGRPKHLESIYRKMQAQGRKFEEIYDLLGARVMVH
                     DKADCYRVLGVVHDLFTPVHERFKDYIATPKSNLYQSLHTTVVAPGGLMVEFQIRTQA
                     MHQIAELGVAAHYRYKEGGAAGDAELLAKLAPVLRNPEWQSTSSDPVEFLDLLKTSLY
                     QDEVFVFTPRGELRSLPRGATPIDFAYQVHTEVGNHCVGAKVNNRIVPLRYELRSGDK
                     VQIITSPMGKPNRDWLKVVKSPGARTKIRHWLKVSEHADSVTLGKEMLERELRRRRLT
                     PPATPTLDDLARGFGHGDADGLYAALGSGTLAMAQVIQRWFPAGLGPVQRLKEASLEA
                     VRAITGRPGRGVRIQGVDNLMVSFARCCQPVPGDSVVGIVTRGRGVTVHRTDCPNTFE
                     DRVAPERRVIVDWSAAEEDAFSVKLSIFGVDRKSLLADIAKAIATTNTNIRTAGIKAN
                     DRNARGAFVVEVRDLAHLRQVMRAVEQVEGVEAVEREQVFGRSRGGESGMAS"
     gene            complement(9958..>10652)
                     /locus_tag="VFR25_04880"
     CDS             complement(9958..>10652)
                     /locus_tag="VFR25_04880"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET9940414.1"
                     /translation="LEQLRRIGPELPPAIVLWSEEWHPGVLGIVASRLTERFHRPTVL
                     ISADEDEGKGSGRSIPGFDVCQALQECREHLIGFGGHSYAAGLTIRAEQMQAFRVRLC
                     DVVTRRLMPDDYVPKLSIDGPLSLDDCNEELVEFLDRLSPFGIGNAEPLFIANDVRLA
                     SPPMVVSRNHLKLSLRQNGREIDCIGFGMGHLAGPIQSDPGRVSIAFVPTINVWQNRS
                     RLQLKLRDIQIR"
BASE COUNT         1611 a         3664 c         3593 g         1784 t
ORIGIN      
        1 tccccagggc gatccggcgc acatcgaact cctccgccgc ggcgacgagc gcctcggcat
       61 tcgagccggc ggcgagcgag acgagccgga tccggtcatg gaactcgcgc gcgatggcga
      121 gcgcggaggc gccgatcgat ccggtcgcgc cgaggagggc gaccttgatc acggcgtcac
      181 cacctggaag aggaggtagt agtagacgat gggggcggcg aagaggaggc tgtcgaaccg
      241 gtcgagcaca ccgccgtgtc ccggaatcaa cctggaggtg tctttcagct ccgcgtcgcg
      301 cttcaagagc gactccacga gatcgccgag ctggccgaac acgcccacga gcgcgccgag
      361 caccacggcg tcgagcgccg tcaggtagtg cgcgaaccag atccgcgcca ggaaggcggc
      421 tccgatcgac gcgagcaggc cggcgatggc tccttccagc gatttcccgg ggctcaccga
      481 gggcatgagc ttggtccgtc cgaatgcgcg ccccaccacg taggcggccg tgtcgcagga
      541 ccagacgagc atgaacggga ggagcgcgta ggacatcccc tcgtggtacg ggcgacgcgt
      601 cgggaacggg agctcgcgga gcgcacccag atgcgtgccg agccagccga tgtagaggaa
      661 tccgaggagc gtcgccgcgc tgttcgcgac cgcctgcttc cctgcaccgc gccgaagctc
      721 cgcgagaagg accgcgccga cgaggatcgt gaagaatccg ccgacatgcc actcctccac
      781 ccgatgcgtg cgaaagcgga ggtagacccc gatcggaagg aggagcacgg cgagcatgcc
      841 cgacttccag tggggtgaga ggcctttcgc ctcgaggagc agatagaatt cgcggaggcc
      901 gagtccgacc acggccatcg tgaagaggag gtacggcacg ccgccgatcc acgcaagcag
      961 gaccaggagc ggcaggaaca gagccgcact caggagccgc gcgggtagcg aggcgtcccg
     1021 ggtcgccgcg gcgacccgcg gcggttcaat cggcgcggcc gaagcgccgg ctccggtgct
     1081 ggaagtcata caccgcctgg tagaggtgtt tccgacggaa gtcgggccac agggtatccg
     1141 tgacccagaa ctcggagtag gcggcctgcc agagcaggaa gttcgagatc cggagctccc
     1201 cactcgtgcg gatcaggaga tcggggtcgg gcagtccgtc cgtatccagc cggtggcgga
     1261 agaattcctc gtcgatcgag gcgagcggga tgcggcgctc gcgatccgcc tccacgatgc
     1321 gcctcgcggc gtccacgatc tcggcacgcc ccccgtagga gagtgcgagt acgagcgtca
     1381 tgccggtgct cgagtcgagg aacgtgcggg tctcctcgag gatctggcgg ctgtgctccg
     1441 ggaggaggtc cacccggccg atcgtgcgga gccggacgtt gttcttcttg agctcgtccc
     1501 gctcctcgat cagcacctgc tcgaggattc gcatgagccc cttcacctcg cccttcgtcc
     1561 gctcccaatt ctcgagcgag aaggtgtaga gggtcaggac cttgaccccg agctcgacgc
     1621 acccctcgac cacttcacgc acggagcggc gtcccgcgcg gtgccccatc agacgcggca
     1681 gcccgcggcg cttcgcccag cgtccgttcc cgtccatgat gatcgcgatg tgaccgggga
     1741 ccggacgccg caggacctgc tcgagcgtga ccgcgtggct ggaaggcttc atcgaagtcg
     1801 gcacctggag ggccgcgacg ggctcggctg tggcctcgtc cgacacgggg gcggcggccg
     1861 tctcatcgtc ccggcccggg ggctggcgga ggacggcgcg tcgcggtagc aggatcagac
     1921 ctccatgacc tcggcctgct tcttgtggaa gatctcgtcg agaaccgcga tgtaccggtc
     1981 cgtcaccttc tggacctggt catgggtctt cttgccgtca tcttcggaaa tcttgtgctc
     2041 ccgttccagc tttttcaatt cgtcgttgct ctcccggcgg acggttcgga cgcgtacccg
     2101 cccgtcctcc acgatcttgc cgagcacctt gaccaggtcc ttgcggcgct cctcggtgag
     2161 gggcgggagg gccaggcgga tgaccgtgcc gtcgtccacg ggattgagcc ccaggtccga
     2221 cttctggatc gccttcacca cctcggggac gatgttgcgc tcccacggct ggacgacgag
     2281 gagccgcgcc tcgggcgcgc tcacgctcgc gacctgctgg agcgggaccg gattgccgta
     2341 gtactcgacg cggatggaat cgaggagcga cgtcgtggcc ttgctggtgc ggatcgaggc
     2401 gacctcgtgc cggatcgtct cgaccgcctt cttcatccgt tcttcggcgt gcttcacgaa
     2461 attcgggtcc atcccttaga cctccacggc cacccgggtg cccaccgctt ctcccttcaa
     2521 ggcgcgggcc agattgcccg gaacggtgac gttgaacacg aggagcggaa ggtggttctc
     2581 catccccatg gtgaacgccg tctgatccat gacgctgagc ctgcgatcga tcgcttcttt
     2641 gtactgaagc acgtcgaagc ggcgcgccgc gggattgcgg cgcggatcct cggtgaacac
     2701 tccgtccacc ttggtcgcct tcaagagcac gtccgcgccg atctcgaccg cgcgaagaac
     2761 cgcggcggtg tcggtcgtga agtagggatg ccccgtgccc gcggccagga tgaccacgcg
     2821 ccccttctcg aggtggcgca ccgcgcggcg gcggatgtag agctcggcga gggtccgcat
     2881 ctcgatcgcg gagaggacgc gcgtcgcgat cccgcggcgc tcgagcgcgt cctggagcgc
     2941 gagcgcgttc atcacggtgg cgagcatccc catgctgtcg gcggtggtcc ggtcgatctg
     3001 gtcgccgtgc gccgcggctg cggtcgcgcc gcgcatcagg ttcccgccgc cgatcacgat
     3061 gccgatctcg gcgcccccgt cgtgcccttc cttgatctcg tcggcgacgc gtcccagagc
     3121 atcgggatcg atccccatgc cctggtcacc ggccaggatc tcgccgctca gcttgacgag
     3181 aacgcgccgc gcgggttcca cggggatcag gcgtccccga tccggaactt ggagaagcgc
     3241 cggaccacga tgttctcgcc catcttcgcg atcgcctggt cgaccagctc cttcaccttg
     3301 cggtccggat ccttgatgta cggctgctcg aggaggcaga tctcctcgta gtacttctcg
     3361 atcttgccgt cgacgatctt ctggagcacc gcctcgggct tgccggtcgt gagagcctgc
     3421 tccatcaaga tggcccgctc gcgctcgatc atgcccggat cgatgctgtt ccgatccacg
     3481 gcctgcgcgt tggtcgcggc cacctgcatg gcgatattcc ggacgagatc gcggaagtcg
     3541 tccgtgcggg ccacgaagtc ggtctcgcag tcgacctcga ggagcacccc gagttggtcg
     3601 ccgggatgga tgtaggcgtg gatgaggccc tgccgggcct cgcggccggc cttcgacgag
     3661 gcgcgggcca tgcccgactt gcggagcacg tcgatggcct tctcgaggtc gcccgcgacc
     3721 tcggacaggg ccttcttgca ctccatcatc ccggcgccgg tccgctcgcg cagctccttg
     3781 acgagcgagg cggtgatcgt cattcgaaaa ctccttcctt cagtcggttg gtgctacggg
     3841 agaggacgtc aggcgttcgc gaccgcgctc ggctccgcgg gcgcttcctt cgcggccagc
     3901 tccgcggcca tgtcggcgcc ttcctgagct tcctggcgcg cggacatgag cgtgtcgctc
     3961 acgaagcgcg cgaacacgcg gatcgcgcgg atcgcgtcgt cgttgcccgg gagcggatag
     4021 tccaccacgt ccgggtcgca gttcgtgtcc accacgccga tcaccgggat cccaaggcga
     4081 ttcgcctcgg ccaccgcgat gcgctccttc ttcgtgtcga tgacgaacac ggccgacggg
     4141 agctgcgcca tctccttgat gccggtgaag atcttcacga ggcgggtgcg ctccttgttg
     4201 atctgcgcct gctccttctt cgagagcttc tcgagcgtcc cgtccgcgtc catcttctcg
     4261 atttccttga agcggcggat gttcgacttg atggtcttga agttggtgag catgccgccg
     4321 agccagcgct cgttcacata gggcatggtg cagcgcccgg cctcttcgag tatcgcgtcc
     4381 ttggcctggc gcttcgtgcc cacgaagagc acgctgccgc tcttcttccc gatgccctcg
     4441 aggagctggc gcgcgtcgtg cagcgccttg agcgtcttct ggagatcgat gatgtagatg
     4501 ccgttccgct cgatgaagat gaacttcttc atcttgggat tccagcggcg ggtctgatgc
     4561 ccgaagtgga ctcccgcctc gagcagttcc ttcattccga tatcggccaa acgaacaccc
     4621 tccggtcttg atcctccgcc ccatcgatcc gcgcggtgtc ccgaaggtcg ggagaccggc
     4681 cgtcgcggcc cggagcgtgt gaagtagcgg ccccgagcgg ggccgcggct tacgactgtg
     4741 ctgcgattac cgcttggaga actggaagcg cttgcgggcg ccagcctgtc cgtacttctt
     4801 gcgctccttc atccgcgcgt cgcgggtgag gaagccttcc ttgccgagga gcccgcgaag
     4861 gctcggatcg gccgtctgca gcgcccgcgc gacgccgagc gagatcgctc ccgcctgccc
     4921 cgcgtgtccg cccccgtgca ccgtcgcgtg gatgtcgtac ttctcgacga gattccccgc
     4981 caccagcggg ccgatggcct gccggctcag cgtgatccgc gagaagtagc ggtcgaggtc
     5041 gacgccgttc acgagtctct tgccggtgcc cggcacgagg cgcacgcgcg cgaccgaggt
     5101 cttgcgccgc ccggtgccct gaacatggtt cgccaaagtt actctcctcc cttgagccgg
     5161 ccatccaacc acggaaacgg acgcggctgc tgcgccgtgt gcggatggtc ggatccggcg
     5221 tagaccttga gcttcttgat catggcgcgg ccgaggcggt tcttcgggag cattcccttc
     5281 accgcgagcc ggatgatctc atccggcttc tgcgcctgaa gatcgcgaaa gcgcgtgagc
     5341 gtgtgtccgc ccggatagag cgagtggcgg aagtacgtct tgtcatcgcg cttgcgtccg
     5401 gtaacgcgca ccttttccgc gttgatcacg acgacgtgat cacccacgtc caggtgcgtc
     5461 gaataggtcg gcttgcgctt ccccttgagc acgctcgcga tgtcgctcgc aagcctgccc
     5521 aggatctgtc cttcggcgtc cgcgacgtac cagtcctgta cgatctccgc ggcgcgcacg
     5581 gaatacgtgc ccattccctt cctccaagtg tgagacgaat cgagcgcaag agattatcag
     5641 gggcgggttt tggtgtcaac cacgcccccc agatcgtagg ccggtcccct ctggaccccc
     5701 cgctccgtga cgatggcccc gatccagcgg gccggggtca cgtcgaaggc ggggttctcc
     5761 gcccgtatcc ccgtgggggc gaggcgcatg ccccggatgg ccgtgacctc ctcggccgac
     5821 cgcgcctcca cggcgatctc cgccccgtcg gggatccggg ggtcgatcgt ggacgtcggg
     5881 gccacgacca cgaacgggat ccccgccgca tgagcggcta gggccagccc cagcgtcccg
     5941 atcttgttgg ccgaatcccc gttccgggcg atccggtcag cccccaccag gaccccctcg
     6001 atccccccgc ggaggatcgt gtgggcggcc gcggaatccg tgatcagccg gaacggcacc
     6061 cccgcctccc gaagctccca ggcggtgagc ctggcgccct ggaggagcgg ccgggtctcg
     6121 gtggcccaca cttcgatgcc gttcggtcgc ctccggtgga gctcgaggag caccgcgagc
     6181 gccgtgcccc ctccaccggt cgcgagcgcg ccggtgttgc agtgggtcag gaagcgggtc
     6241 tgggtcggaa agagcgatgc cccgtgctcc gccatggcgc gcgaggccgc gcgatcctcg
     6301 tcccagatcg cgagcgcctc gtcgcgcacc cgcgcggtca ggttctcgga gtccttcgcg
     6361 acggcccggc gcgcggcgcc gagcacgcga tcgaccgccc aggccaggtt gaccgcggtg
     6421 gggcgcgagg cgacgaggag ccgtccggcg tcctcgagcc actcgagctg tttggaggcg
     6481 ctcccctctc gagtcgcttc gaccgcgagc gcgagcgcgg ccgcgatgcc gatggccggc
     6541 gctccgcgca ccgcgagccg ggagatcgcg tccgccatcg cgcgcgcgtc cctgatctcg
     6601 atcatcacct cgcgatcggg gaggagcgtc tggtcgagga ggagaacgcg gtcgccctcg
     6661 aagcgaaaag gatcccggct cacacgcgtc cggctcgctc gacgagcagt ccgaggagat
     6721 gcttcaagta gctgagcggg agtccgacca cgttcgagta ggacccttcg atcgcctcca
     6781 cgaacacggc ccccttcccc tgcaccgcgt aggcgcccgc cttgtcgagc ggctcgcctg
     6841 tggccacgta gcgcgcgacg tcgtcggcat cgagcggcgc gaacttcacg atgctccgga
     6901 gcgcgccgcc gaccgcgcgt cccgcgacgg gatccaggag gaagaggccc gtccagacct
     6961 cgtggatccg gcccgaaagc cgctcgagca tgcgcgccgc ctcctcgggt ccggatggct
     7021 ttccgagaat cgcgcggtcg agcgagacga tggtgtccgc gcccacgacc accgcctcgt
     7081 cgtcggcacc gttgccgccc cgagcctcgg ccgcggctct cgccttggct tccgcgagcc
     7141 gctgcacgtg cgcatcggga cgctcccccg gaagcgcgga ttcgtccacg tccacgggga
     7201 acgtcgtgaa tcgggcgccc accagggtga gcagctcgac gcgcctcggc gagcgcgagg
     7261 cgaggatgag cttggggccg agcggattga tcagattggt ctgcatgccg ctaatccgtg
     7321 gtttcgagca ggaacgtcgc agggccgcgg ttcacgagct cgacttccat gctcgcgccg
     7381 aaccgtcccc cctgcgtggg aacgccgcgt gcggccagcg cgtcgcagaa ccggcgccac
     7441 gcgggctccg ccaacgtggg tgccgcggcc gcttcgaagc cgggccgccg tccgcgcgac
     7501 gtgtccgcgt agagggtgaa ctgcggcacg acgagcgctt cgccccccag atcgcgcacc
     7561 gagcggttca tctttccggc ctcgtcctcg aagatccgga gctccgcgca gcgctccgcc
     7621 aggcgctcct ccgcgccctc cggatcgccg gcccgcacgc cgaggaggat gaggagtccc
     7681 ggcccgatgc gcgagatctc cgctccgtcc acgcgcaccg ccgcgcgcga cacgcgctgg
     7741 acgagcgccc tcacgaggcc atccccgatt cgcccccacg cgaccggccg aacacctgct
     7801 cgcgctccac cgcctcgacc ccttcgacct gctccaccgc ccgcatcacc tgacgcagat
     7861 gcgccaggtc gcggacctcc accacgaacg cgccccgcgc gttccggtcg ttcgccttga
     7921 tgccggcggt gcggatgttc gtattcgtcg tggcgatcgc cttggcgatg tcggcgagga
     7981 ggctcttccg atccacgccg aagatcgaga gcttgaccga gaacgcatcc tcctccgcgg
     8041 cgctccagtc gacgatcacg cgccgctccg gggcgaccct gtcctcgaac gtgttgggac
     8101 aatcggtgcg atgcaccgtc acgccgcgac cgcgcgtcac gatcccgacc accgagtcgc
     8161 ccggcaccgg ctggcagcac cgggcgaagc tcaccatcag gttgtcgacg ccctggatgc
     8221 ggacgccgcg tccggggcgc cccgtgatcg ctcggaccgc ctcgagcgat gcttccttga
     8281 gccgctgcac cggaccgagc cccgccggga accagcgttg aatgacctgc gccatcgcga
     8341 gcgtgcccga gccgagcgcg gcatagagtc catcggcgtc gccgtgcccg aagccgcgag
     8401 cgagatcgtc gagggtgggt gttgccggag gcgtgaggcg gcggcgcctg agctcgcgct
     8461 cgagcatctc cttccccagg gtcacgctgt ccgcgtgctc cgagaccttg agccagtggc
     8521 ggatcttggt tcgcgcgccg gggctcttca cgaccttgag ccagtcgcgg ttcggcttcc
     8581 ccatcggcga ggtgatgatc tggaccttgt cgccgctcct tagctcgtag cggagcggaa
     8641 cgatgcggtt gttcaccttc gcgccgacgc agtgattccc cacctcggtg tgcacctggt
     8701 aggcgaagtc gatgggcgtc gccccgcgcg gcaggctgcg gagctcgccc cggggcgtga
     8761 agacgaacac ctcgtcctgg tagagcgacg tcttcaggag gtcgaggaac tcgacgggat
     8821 ccgagctggt cgactgccac tccggattcc ggagcacggg agcgagcttc gcgaggagct
     8881 cggcatcccc cgcggcgccc ccttccttgt agcgatagtg cgccgccacg cccagctccg
     8941 cgatctggtg catcgcctgc gtccggatct ggaactccac catgagaccg cccggcgcga
     9001 ccacggtcgt gtggagcgac tggtacaggt tgctcttggg agtcgcgatg tagtccttga
     9061 agcgctcgtg gaccggcgtg aagagatcgt gcaccacgcc gagcactcga tagcagtccg
     9121 ccttgtcgtg caccatcacg cgtgcgccca agagatcgta gatctcctcg aacttccgac
     9181 cctgcgcctg catcttccgg tagatcgact cgaggtgctt cggccgtccc acgacgtcgg
     9241 ccttcatgcc gaggtggacc agcctcgact tcacaggctg gatcacgtcc tggatctgcc
     9301 gctcgcgctc ctcgcgcttc agctggacct tgtcgcggat ctcccggtac gcgtcggggt
     9361 cgaggtgctt gaggcagaga tcctcgagct cccacttgat ccgcgcgatc ccgagcctgt
     9421 gtgcgagcgg cgcgtagatc tcgcgcgtct cgcgcgcgat ccgatgcgcc cgcgcggggc
     9481 cgagcgcctg gagcgtgcgc atgttgtgga gccggtccgc gagcttgatc aggatgacgc
     9541 ggaggtcgcg cgccatcgag aagagcatct tgcggaagtt ctcggcctcc tcctgctcgg
     9601 ggcgctcgaa gtggagatgg ccgatcttgg tgactccgtc gacgagggcg gcgacgtcgc
     9661 gcccgaaccg gcgctccagc tgcggaagcg tgatgggagt gtcctcgacc acgtcgtgga
     9721 ggagcgcggc ctggatgagg acctcgtcga cgccggaccc gaggagatcg gcgaggatcg
     9781 tggcgaccga ggccaggtgc gtgacgtagg gctcgccgct cttccggacc tgggagccgt
     9841 gcgcgtcgac gacgaagaga taggcggccg cgatcttctc gagatcgagg gcgcggttgg
     9901 accgctggag tgcttcgagc agcggtcccg ggagatcggc agggaccgga accatggcta
     9961 tcggatttgg atgtctcgga gcttcagctg gagccgggat cgattctgcc agacgttgat
    10021 ggtgggaacg aaggcaatcg aaacccggcc cggatcggac tggatcggtc ccgccaggtg
    10081 gcccatcccg aagccgatgc aatcgatctc gcggccgttc tggcggaggc tcagcttgag
    10141 gtgattccgg ctcaccacca tgggaggcga ggcgagccgc acgtcgttgg cgatgaacaa
    10201 gggctcggcg ttcccgatgc cgaacgggga gagccggtcc aggaactcaa ccagctcctc
    10261 gttgcagtcg tcgagggaga gcgggccgtc gatggagagc ttgggaacgt agtcgtccgg
    10321 catgagccgg cgcgtcacga cgtcgcagag ccgaacgcga aaggcctgca tctgctccgc
    10381 gcggattgtg agcccggcgg cgtagctgtg gccgccgaag ccgatcaggt gctcgcgaca
    10441 ttcttgaagc gcctggcaga cgtcgaatcc ggggatgctc cgccccgagc ccttcccctc
    10501 gtcctcgtcg gccgaaatga gcacggtcgg gcggtggaag cgctccgtga gacgagacgc
    10561 gacgatgccg agcacgccgg gatgccactc ctccgaccag agcacgatcg cgggggggag
    10621 ctcgggacca atacggcgca attgctcgag cg
//
LOCUS       DASTKC010000118        20084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_796129, whole genome shotgun sequence.
ACCESSION   DASTKC010000118 DASTKC010000000
VERSION     DASTKC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 20084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20084
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796129"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFR25_04885"
     CDS             complement(<1..748)
                     /locus_tag="VFR25_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HET9940415.1"
                     /translation="MYGRIRRIHLVGIGGSGMSGIAEVLLNLGYEVSGSDVKEGEAVS
                     RLRGLGARIAGSHAAANVDGADVVVASTAIGTGNPEVDRAHALGIPVIPRAEMLAELM
                     RIKVSVAVAGAHGKTTTTSMVGDILARGDLDPTVIVGGRLKAVGANARLGRGPFLVAE
                     ADESDGSFLLLSPTIAVVTNIDLEHLDFYKSLDDIRGAFLRFANRVPFYGTVVLPADD
                     PHAALIRGDVHRRVVTYGFEHEAEFRGHDLE"
     gene            complement(753..1889)
                     /gene="murG"
                     /locus_tag="VFR25_04890"
     CDS             complement(753..1889)
                     /gene="murG"
                     /locus_tag="VFR25_04890"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9940416.1"
                     /translation="MADSAVPVRLLVAAGGTGGHVYPGIAIAEEWMRLHPDSQVTFVG
                     TARGVEAKAVPAAGFTFRPIEARGFPRRPTPAALGALWSFGRGFMQMAGIIKEVKPHV
                     ILATGGYVSGPAAIWARLLSIPLVLQEQNSVPGAANRWLSLIASQVHISFVESRNYFR
                     RKNHLKVTGNPIRRSLLRQDKAGAYEALGLDPSRRTLLVFGGSRGAASINRAVAGALP
                     LLARVPSLQVLWQTGSEDEAAMIEASRTAPFPVRVRAYVDRMEEAYAVADLAICRAGA
                     MTITELTACGVPAILIPYPHAARDHQTQNARGLVDRGAMEMIEDKDLNPAEVASRVEA
                     LFRDESRLRRMARNARAFARTNAAEKIARSIEALAGLAGPVDEI"
     gene            complement(1879..3045)
                     /gene="ftsW"
                     /locus_tag="VFR25_04895"
     CDS             complement(1879..3045)
                     /gene="ftsW"
                     /locus_tag="VFR25_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9940417.1"
                     /translation="MSQLAVPRIDRTLLLTVAGLTALGIVMVYSSSAFLGAERFQSEY
                     FFLKRHIVRVVLGLALLFALARVDYHVYARFAPWAFGGSVALLIVLVALGGEGIRGAN
                     RWLSLATINIQPTELARVACVVYLARLLDRKGDRMASFRDGVLPAVLTLGLLSVLILL
                     QPNLGSTIALLTTGFVMLHLAGARTRHLGILVLCGVLAAWVQVLRHPYMMERVAAWRG
                     LWGGSIDALGRNWQLSQSILALGSGGVLGTGPGHGLQKVFFLPDPHTDFIFAVIGEEL
                     GFVGTALVLAAYLALFLRGLKIAGSAPDRFGFLLAAGLTVNLSVYVAMNIAVVTGVIP
                     TTGLPLPFLSYGGSALVVNLGVIGVLLNIASQMESGIRARAFDAPIRSRRPGGR"
     gene            complement(3042..4439)
                     /gene="murD"
                     /locus_tag="VFR25_04900"
     CDS             complement(3042..4439)
                     /gene="murD"
                     /locus_tag="VFR25_04900"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9940418.1"
                     /translation="MTTTARTQAPWFAGARALVVGVARSGSAAARLLLRHGASVRAID
                     RRRRDEIAGDAAELEGRGVEVRYGTMDASALEGCDLVVTSPGVPADLPLFVEAARRGI
                     PVAPELELGFAVARAPILAVTGTNGKSTTVELLGAIGRNAGKRTEVLGNIGTALSERA
                     EDVPEDGWLVVEVSSFQLELCTRFRPHVGILLNVTPDHLDRHGSMERYAEIKARLFAF
                     QNEHDFRVQPLGDSRLTRLLEPMRSAPIWFGFADPTGDGVWEEKGVLEFRFEGREGMI
                     VKREEALLRGPHNTENICAAAGAALAAGVSERVVAKTLKEFRGLPHRLTLVAEIEGVR
                     YINDSKATNVDAMKRALESFADPITLIAGGRDKDGDFDAIASLVHERVQHAVYVGEAT
                     SKLERAFSQVPSTRAKTLEEAVTLARSHATPGGVVLLSPGCASFDMFRNFEERGARFE
                     AAVMELKRSIERGRR"
     gene            complement(4436..5524)
                     /gene="mraY"
                     /locus_tag="VFR25_04905"
     CDS             complement(4436..5524)
                     /gene="mraY"
                     /locus_tag="VFR25_04905"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9940419.1"
                     /translation="MLYHFLYPLHVYFTPLNVFRYITFRSAYALATALVIALVLGPPV
                     IKKLRELKIGQKVRDDGPQSHLPKAGTPTMGGILIVIAILVSTLLWGNLGNRNVLIAL
                     VATLWMGTVGFIDDYLRVVKHYRKGLLGRYKLAGQILLGIGIFFAVYFYPSPGILNPG
                     ETNIPFLKQTVIHLGWLYLPFVVLVVTGASNAVNLADGLDGLAAGLTAFAAVALGGMC
                     YITGHVKFSGYLQVPYIADTGELTVFCAAVIGATLGFLWWNCHPADVFMGDTGSLSLG
                     AALGTVAVLIRREFLLAIICAVFVAEALSVMAQVLSFKIMGRRILRMAPLHHHFELSG
                     WKESRVVVRFWIAAALAALVSLSTLKLQ"
     gene            complement(5524..6978)
                     /gene="murF"
                     /locus_tag="VFR25_04910"
     CDS             complement(5524..6978)
                     /gene="murF"
                     /locus_tag="VFR25_04910"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET9940420.1"
                     /translation="MSRLAGIPWMTVEDVLAATGGTLKRGAAGTGLTFRGCAIDSRTI
                     QDGELFVPLPGSNVDGHEFVASALEGRAAGSLVARGASWSSGSAPSSKPVIEVEGALA
                     ALQAIGRHCRERAGVPTVAVTGSNGKTTTKEMIAAVLGARLHVHRNVGNLNNHIGVPL
                     TLSHLTPEHQVLVIEMGMSARGEIALLASLASPEVGVITNASAAHLEQLGSVEEIARA
                     KSELAEALRPDGLLVVNADDALLWAMNRGRPVTVRGYALDNQDADLRPDSVVESGDGG
                     TRFILPDGTEVTLALLGRHNVRNALAAILVGDRFGIPRGEAARALGALRPAKHRLERV
                     TTPGGVNVIDDAYNANPASMKAALALLGSMDARGARRAVLGDMLELGPEAARLHEDVG
                     RAVPKGAWLYAAGGHAADLERGARAAGVDAARIRRFDDVPAMAQSVARDARAGDLVLV
                     KASRGMRLERVVEALMGASGTGHGAASPAPAGRA"
     gene            complement(6975..8498)
                     /locus_tag="VFR25_04915"
     CDS             complement(6975..8498)
                     /locus_tag="VFR25_04915"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET9940421.1"
                     /translation="MHAGPLAPRRLIEASARHAAQIARIVGAVEVHGSPRGVWERVQY
                     DSRAVGSGDIFVAIRGQKTDGHAHLRQARDQGAVAAVVETVTPGLDLPQIRVTDSRRA
                     LALLAAEETGHPSRELVMIGVTGTNGKTTTTHLIAAELEARGERVGVIGTVGYRFGDQ
                     TEPAPHTTPEAPDLARLLRRFRDRGATAVAMEVSSHALALDRTYGVAFDAGVFTNLTQ
                     DHLDFHGTMEAYRDAKARLFRAETRGDSSKAFTGAVNTDDPEGRWIREHGDPPMLGFG
                     LEPGAEVTAEDIRWERSGTRLRIRSPQGSSSVALRLRGAFNVMNALAAFAAGLAVGVP
                     VTAITAGLESVASVPGRLEPVEAGQPFLVLVDYAHTPDALLRALDAVRATGPKRLLCV
                     FGCGGDRDRGKRPLMGRVAAEHADEVFLTSDNPRSEDPRAILAEIEAGVRGAAHVHTI
                     VDRTEAIGAAIQACADGDALLIAGKGHETYQILGSRTIPYDDREVARKALETREYRA"
     gene            complement(8465..10564)
                     /locus_tag="VFR25_04920"
     CDS             complement(8465..10564)
                     /locus_tag="VFR25_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9940422.1"
                     /translation="MIPNDARRLRLLAVVGFAAFLMLLTRLVQIQVVQHESFAKAARQ
                     QQTRRVLLEPERGRIFDRHLRSLADNVALSRLSVRPRDVKNARAIQTFLAKAAGANAV
                     SRFKAGRSRHAAYIRLSSQLSPQQELALQATTLPEGLRVEAVPGRVYPLETIARSVVG
                     VVGSEGDGLEGLERVYDRELKGKSGWATLFCDARGAAYELPRSLVKLPESGESIVSTV
                     DLDAQSIAAIELKKAVEKNGAKGGMAVFADPRTGDILAMVTVDAAGRTDSDHNRNRVI
                     ADQYEPGSTFKVLAGCAALEERLLQPSDSFYVDKGEADVGGFTIHDSHPETRWYTFRD
                     ATALSSNVCYAHIGTKVGAKTLYGYARLFGFGQPTRIMLPGEAPGQIRHPDRWSGRSL
                     ATISIGQEVLVTPLQILMAYCAIANGGELLRPRVVSTVVDDQGAPIREVPVEHVRRVV
                     SSETARTFRSFLRDAVVSGTATEAALPWCEVAGKTGTAQKALENGRGYGAGSYISSFV
                     GMAPYENPSVVGLIVIDEPRGGLYYGGSVAAPVFRDILAAWALQGRGPIALPPSSVVR
                     GEDEPKARMAVPDVRLLELDRAREVLARAGDGLRVASGGAVGGRVMAQTPAPGTEIGS
                     GAVIELALATGGDGETTVPDLRGLALRDALARLTASAIEVARVEGSGTVVRQSLEAGK
                     TVKKGTRCTLVLSPRGA"
     gene            complement(10561..11067)
                     /locus_tag="VFR25_04925"
     CDS             complement(10561..11067)
                     /locus_tag="VFR25_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940423.1"
                     /translation="MSLTLERGLPRDVTPGAKERPDQAYLRRYTVRGRRVRYGKTLLI
                     GLAALLLLFARVWENTVAHATAMDRDRLRREVRALENRLQLSSEVSEQSSLLSGVDPR
                     SLRALGFVAPDPAHIVDIDLKDSLPHAMPGGVAARVSSWFQGLKRRNATPAVPGVDAV
                     PVRAQVGP"
     gene            complement(11064..11978)
                     /gene="rsmH"
                     /locus_tag="VFR25_04930"
     CDS             complement(11064..11978)
                     /gene="rsmH"
                     /locus_tag="VFR25_04930"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9940424.1"
                     /translation="MPGHVPVLKQEAIRWLVTDPEGVYVDVTLGLGGHSSELVAALGG
                     GGRVIALDCDPAAVERASANPPAPPPRFTPVRGRFSDLDSRLDQIGITEVDGILADLG
                     LSSDQLDDPARGLSFATEGPLDMRLDPSRPMSADALIRGVDDRALERILGSYGELPRA
                     RSAVRAIRRAERAVQPLTTGALRDALAPIYPGPQRPRRLAQAFQALRVAVNHEMEELE
                     ALLATAARRTRTKGRLCVIAYHSLEDRIVKTAFRPPRPLDVWEEPAETPWIPLTRKPI
                     RPTDEEIAVNPRAASARLRVAERKENRR"
     gene            complement(11985..12422)
                     /locus_tag="VFR25_04935"
     CDS             complement(11985..12422)
                     /locus_tag="VFR25_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9940425.1"
                     /translation="MIAIPNGRTALKIDRPTCQWELSRAGGPVRLRLYGVLGARDLDR
                     VVETLRERCRSPRDLACLDFDEVEHLDYRALPEFVSRLGRQRERGASLWMVGLSPYLR
                     ALFQVAGQGPALARLEWGAAEGPAPSLEDGRMSHAEAWDGMVA"
     gene            complement(12419..12859)
                     /gene="mraZ"
                     /locus_tag="VFR25_04940"
     CDS             complement(12419..12859)
                     /gene="mraZ"
                     /locus_tag="VFR25_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET9940426.1"
                     /translation="MATFRGSYQHSVDHKGRVSIPARFRRSLSGDAEGSFVVLRGFDA
                     CVSLFPSDEFKSYEERLRSRSFGDEAARRFQRLFLLDSRDETLDAQGRVAIPPRLLEH
                     AKITKDVLVNGVLDHIEIWDPKVFEQYLASSNRTYEDIAGEILP"
     gene            complement(13064..14389)
                     /locus_tag="VFR25_04945"
     CDS             complement(13064..14389)
                     /locus_tag="VFR25_04945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940427.1"
                     /translation="MKRLLAIAYFFPPLGGGGCQRTLKLVRYLEPEGWASTVVTTRDA
                     DYWILDPSLEAEVPRTAEVIRVGGLTSLRLVRLLANAGGIERRQGARDARAFQRLRRA
                     QSWLLLPDGYRAWAREAERAAVRRIAAGGIDALWTTSSPESAHLAGLAVKRRFGLPWV
                     ADFRDPWVGRVTYAPPTAWHDARHKELERAVVTAADRVTLVSEPMTALYRKRYRDLPG
                     EKFLTLPNGVDSDDWRRAAELPPRAGEGDEGRCVLLHAGQLAHRPTVRTLLDALTRVI
                     EADPGARDQLRLRFLGGNEELRPDEWAARGLDGIVELEPSRPHLEALQAMRRAYALVL
                     LGHGGDADSLLYTGKLYEYLTSGRPILGVLDPGPAADLILEAGAGTVVRSGDVGSTSR
                     AVESLLRSFRAGQDSAVRPPAALAAGWERKAMAGRAATILERLAEPAAG"
     gene            complement(14386..15480)
                     /locus_tag="VFR25_04950"
     CDS             complement(14386..15480)
                     /locus_tag="VFR25_04950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940428.1"
                     /translation="MRILLLAHAPSTHTALWARALAARGHAIRLLSAEPGAPGPVPVR
                     VVGARVPVRALQYASARGAVREEILRFKPDVTVAHFLPNYGFLAALAGVRPWMLVCWG
                     SDLLVNAWRTPLHRARARWTLRRADRIHVDAAVLARAAEELGAPRDRIWTRAWGVDVP
                     SLAPSEAWSARRERSPALRILWTRQLEDLYGPETFVRALGALARRGVSFEATIAGSGP
                     LRMGLERLAKREKVADRITWTGWVDRPRITALYRSHHAYVSLSRSDSTSQSLLEAMAA
                     GLVPVVTDIEGNLEWVQHRKEGLLVPVGDEEAVAAALAEVAQGAASAEAMSERAASVA
                     RERGNLADTVDLIEQQLLDLAGASPARAVS"
     gene            complement(15485..16459)
                     /locus_tag="VFR25_04955"
     CDS             complement(15485..16459)
                     /locus_tag="VFR25_04955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940429.1"
                     /translation="MKSDALPAEIMAPNPEAWDDLVQASPGGSVFHTAAWARLWTGLK
                     SSTKWEAAILEEGGRYVGALGWIARRHGWFESLDAMPYATYGGPIVREGHLDAPRARR
                     ALIETFLAHAARRRVLRAQLTWYGGRPEEFPEESPPDEGFTHVLALEGDYERVADRFS
                     PSTRRLVRQSDESGLSIRAAESAADVAQFIEIAEETVVRRGGKPKPRALYERIQGDLV
                     TAGLARYHLVEHEGKAVAGSLHLFHQGVATSWLPVSRESSWPLRPNNFLVASLLETLC
                     QAGYTEYNFGASPSDAAGLIRFKEGWGARPRPVWTLTRRSAIHRRLRP"
     gene            complement(16456..18636)
                     /locus_tag="VFR25_04960"
     CDS             complement(16456..18636)
                     /locus_tag="VFR25_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET9940430.1"
                     /translation="MLLAGWLLAAPAGAATPIFITNHVHSDQVGAMAVWQGRIVAATL
                     GGLVFVDPATQVRTKLLASPSGLPSNQVLAVAVGPSGILWAGTADKGVARLVPGGGFK
                     RTLTSFDGLPSDHVQTLYVHGDSVWVGTSGGVALFTESAGGQTTLRRSDSRASTGGGL
                     VSDDVLAFQQVADTLWVGTSAGLSTFASGVWQNRASTLAVAARDLAVHQDTLWAATAA
                     GPRRYAGGAFTVVAVGHIGSSLALFTDGVRLLSGSAVQGAYRYTGGAWSAFGSIPPGV
                     RLSSFVTAPDGSLWAGSDRGTARYDAGTDTWTLYPTDGPAVNGAERALVDVRGAWFAT
                     GNAVPPGGSLGNVLHYDGTTWSLLSPATTGGALQSTSVFGLAEDPLGAIWMGHCCSTV
                     DPLPRTDRYDPDTGVWNAPGSTNIFVITRGPDALMYEGSVEHGNGVYVYDPTVLAVVD
                     SLTPLNTQGSAIGTGLASNNLRGISFDTTGRGWIAHAATGLDIWDGRGTLTDHSDDVW
                     KHLATGFPSVQATAVETDGASSGWVGTVAGVVRIVNDVVVPGTTTAINAALPSTAVRD
                     LELDSEGNLWVATTSGLARVASGSTFVEHWSTAEGLAGDDVRALAWDAAKRTLWVGTT
                     SGISEVVVPKAGSTTFTSQSYVYPSPIGPETTALRIGGITGEVTGEIRDVTGAIVRRF
                     RCDPAHDVVWDLLLADGTRARSGVFVVVIRDREEHRMLRVAVVR"
     gene            complement(18666..19838)
                     /locus_tag="VFR25_04965"
     CDS             complement(18666..19838)
                     /locus_tag="VFR25_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940431.1"
                     /translation="MRSPKKRVEPAPFSAARRRAPPRARRNRESRPAGRALPWFDRPV
                     FPAESYAGLLYFTVGVTIAALAMNGLYRRTDFTDRIERAFLVGKSVVQAVLLLMAATF
                     LFQMPRYSRFLVLLVGPLLFLFLYVARGALASAAAGARKQGFAFRRVLLLGSGAEVER
                     ARAALEGSRREGFEPLHALASSGPDEPPEAAAARIRSLVLGERVQIVCLVPEPDEVPY
                     LLAMGAALRDSGAALWWAGSVAHLAAAAGESRRLGPLDAVLLHAPSRGLSLRARKRAS
                     DLLLSFLIAPWRWGRIRSYLAERGDGLGAGEAWRQVVTGARSWVGRSTYEADRWTGVP
                     PWARLALESLRPGVVSPANGSGVDRRARLESELAYLTRFSIAEDLRVFLRATERKR"
BASE COUNT         3038 a         7182 c         7099 g         2765 t
ORIGIN      
        1 cttcgaggtc gtggccgcgg aactccgcct cgtgctcgaa gccgtaggtg acgacccggc
       61 gatgcacgtc gccgcggatc agggccgcat gcggatcgtc ggcgggaagc acgaccgtgc
      121 cgtagaacgg gacccggttc gcgaagcgga ggaaggcgcc gcggatgtcg tccaggctct
      181 tgtagaagtc gaggtgctcg aggtcgatgt tcgtcacgac cgcgatcgtg ggcgagagga
      241 ggaggaagga gccgtcgctc tcgtccgcct cggcgacgag gaacgggccg cggcccagcc
      301 gagcgttcgc gccgaccgcc ttgagccgcc cgccgacgat gacggtcggg tcgaggtcgc
      361 cgcgcgcgag gatgtcgccg accatcgagg tcgtggtggt cttgccgtgc gcccccgcga
      421 ccgcgacgga gaccttgatg cgcatgagct cggcgagcat ctcggcgcgg ggtatgacgg
      481 ggatgccgag cgcgtgcgcg cggtccacct cgggattccc cgttccgatc gccgtggacg
      541 cgaccaccac gtccgcgccg tccacgttcg cggccgcatg gcttccggcg atgcgcgcgc
      601 cgagcccgcg caggcggctc acggcctcgc cctccttgac gtcggagccc gagacctcgt
      661 agccgagatt caagagcact tccgcgatcc cgctcatccc actgcccccg atgcccacca
      721 gatggatccg acggatccgc ccgtacattc tcctagatct cgtcgaccgg gcccgcgagc
      781 ccggccagcg cttcgatcga acgcgcgatc ttctcggccg cgttggtgcg cgcgaaggcg
      841 cgcgcgttcc gcgccatgcg gcgaagtctc gactcgtccc ggaagagcgc ctccacccgt
      901 gatgcgacct ccgccgggtt gaggtccttg tcctcgatca tctccatggc gccgcgatcc
      961 acgagcccgc gcgcgttctg cgtctggtgg tcgcgcgcgg cgtgcgggta cgggatcagg
     1021 atcgccggca ccccgcaggc ggtcagctcc gtgatggtca tcgccccggc gcggcagatc
     1081 gcgagatcgg cgaccgcgta cgcctcctcc atgcgatcca cgtaggcgcg cacgcgcacc
     1141 ggaaagggcg cggtccggct cgcctcgatc atggccgcct catcctcgga tccggtctgc
     1201 cagagcacct ggagcgacgg cacgcgcgcg aggagcggga gcgccccggc caccgcgcgg
     1261 ttgatgctcg ccgcgccgcg gctcccgccg aacacgagga gcgtgcggcg cgagggatcg
     1321 aggccgagcg cctcgtaggc gcccgccttg tcctgacgca ggagcgagcg gcggatcgga
     1381 ttgccggtca ccttgaggtg gttcttccgg cgaaagtagt tccgcgactc gacgaagctg
     1441 atgtgcacct ggctcgcgat caggctgagc caccggttcg cggcgcccgg caccgagttc
     1501 tgctcctgca gcacgagcgg gatcgagagg agccgcgccc agatcgccgc agggccgctc
     1561 acgtagcctc cggtcgcgag gatcacgtgc ggcttcactt ccttgatgat gccggccatc
     1621 tgcatgaagc cccgcccgaa cgaccagagc gctccgagcg ccgcgggcgt cggacggcgc
     1681 gggaacccgc gcgcctcgat cgggcggaac gtgaagccgg ccgccggaac ggccttcgct
     1741 tccacgccgc gggccgtgcc cacgaaggtc acctgcgaat cggggtgcag gcgcatccat
     1801 tcctcggcga tggcgattcc cggatagacg tggccgccgg tgccgccggc cgccaccagg
     1861 aggcgcacgg ggaccgcgct atcggccacc tggtcgccgg cttcggatcg gggcgtcgaa
     1921 cgcacgtgct cggatgccgg attccatctg actcgcgatg ttgagtagga caccgatgac
     1981 tcccaggttc acgacgaggg ccgagccccc gtagctgagg aacggaagcg gcaggccggt
     2041 ggtcggaatc acgccggtca cgaccgcgat gttcatggcg acatagacgg acaggttcac
     2101 ggtgagcccg gcggccagga gaaagccgaa gcggtccggc gccgatcccg cgatcttgag
     2161 accgcggagg aagagcgcga gatacgccgc cagcacgagg gcggttccca cgaagccgag
     2221 ctcctcgccg atcacggcga agatgaaatc cgtatgggga tccggaagga agaagacctt
     2281 ctggagcccg tgccccggcc cggtcccgag aacacccccg gacccgagcg cgaggatcga
     2341 ttgcgacagc tgccagttcc ggccgagggc gtcgatcgac cctccccaaa gcccgcgcca
     2401 cgccgcgacc cgctccatca tatacgggtg gcgcaggacc tgcacccacg ccgcgaggac
     2461 gccgcagagg accaggatcc cgaggtggcg ggtccgcgcc cccgccaggt ggagcatcac
     2521 gaaccccgtc gtgaggagcg cgatggtgct cccgaggttc ggctggagca ggatcagcac
     2581 cgagaggagg ccgagcgtga ggacggccgg gagcacgccg tcccggaagg aggccatgcg
     2641 gtcccccttc cggtccagga ggcgcgcgag gtacacgacg cacgccaccc gggcgagctc
     2701 ggtcggctgg atgttgatgg tcgcgagcga gagccagcgg ttggcgccgc ggatgccctc
     2761 gcccccgaga gccacgagca cgatgaggag cgccacgctg cccccgaacg cccacggcgc
     2821 gaagcgcgcg tagacgtgat agtcgacgcg cgccagggcg aagaggaggg caaggccgag
     2881 aaccacgcgg acgatgtgcc gcttgaggaa gaagtactcc gactggaacc gctccgcgcc
     2941 taagaaggcg ctcgacgagt agaccatgac gattccgagc gcggtgagcc ccgccacggt
     3001 caggaggagc gtgcggtcga tccgcgggac ggcgagctgg ctcatcgacg cccccgctcg
     3061 atcgaccgtt tgagctccat cacggccgcc tcgaagcgcg caccgcgctc ttcgaagttc
     3121 cggaacatgt cgaagctcgc gcagccggga gagaggagga cgacccctcc tggggtcgcg
     3181 tgggaccggg ccagcgtgac cgcctcctcg agggtcttcg cccgcgtcga aggcacctgg
     3241 ctgaaggcgc gctcgagctt ggacgtcgct tccccgacgt agacggcgtg ctggacccgc
     3301 tcgtggacca gcgacgcgat ggcgtcgaag tccccgtcct tgtcccgtcc tcccgcgatc
     3361 aaggtgatcg gatccgcgaa cgattcgagc gcgcgcttca tcgcgtcgac gttcgtggcc
     3421 ttggaatcgt tgatgtaccg cactccctcg atctcggcga cgagcgtgag ccggtgcgga
     3481 aggccgcgga attccttcaa ggtcttcgcg acgacgcgct ccgagacgcc cgccgcgagc
     3541 gcggctccgg ccgcggcgca gatgttctcg gtgttgtgcg gtccgcggag gagcgcctcc
     3601 tcgcgcttca cgatcattcc ttcccgtccc tcgaaccgga actcgaggac gcccttctcc
     3661 tcccacacgc cgtcccccgt cggatcggcg aaaccgaacc agatcggagc cgagcgcatc
     3721 ggctcgagga gccgcgtgag acgggaatcg ccgagcggct gcacccgaaa gtcgtgctcg
     3781 ttctggaacg cgaagagccg cgccttgatc tcggcatagc gctccatgga gccgtggcga
     3841 tcgagatggt cgggagtcac gttgaggagg atgcccacgt ggggacggaa gcgcgtgcag
     3901 agctcgagct ggaagctcga cacctcgacc acgagccagc cgtcctcggg cacgtcctcg
     3961 gctcgctcgg agagcgcggt tccaatgttc ccgagcacct cggtgcgctt gccggcattc
     4021 cggccgatcg cgcctaagag ctccaccgtc gtcgacttcc cgttggttcc cgtcaccgcg
     4081 aggatcgggg cgcgcgccac cgcgaagccc agctcgagct ccggcgccac cgggatgccg
     4141 cggcgcgccg cctcgacgaa gagcgggagg tcggcgggaa cccccgggct cgtcaccacg
     4201 agatcgcacc cctcgagtgc agaggcgtcc atcgtgccgt agcgcacttc gacgccgcgc
     4261 ccctcgagct ccgccgcgtc tcccgcgatc tcgtcgcgcc gccggcggtc gatggcgcgc
     4321 acgctggcgc cgtgtcggag gagcaggcgc gccgccgcgg agccgctccg cgcgactccg
     4381 accacgagcg cgcgcgcgcc cgcgaaccac ggggcctggg tgcgcgcggt ggtggtcatt
     4441 ggagcttgag cgtgctcagg ctgacgagcg cggccagcgc ggcggcgatc cagaagcgga
     4501 cgaccacgcg cgactccttc cagcccgaca gctcgaagtg atggtgcaag ggggccattc
     4561 ggaggatccg gcgccccatg atcttgaacg agaggacctg cgccatgacc gagagcgcct
     4621 cggccacgaa cacggcgcag atgatcgcga ggaggaactc gcgccggatg agcaccgcca
     4681 ccgtgccgag ggcggcgccg agcgagagcg agcccgtgtc tcccatgaag acgtccgcgg
     4741 gatgacagtt ccaccagagg aagccgagtg tcgccccgat cacggccgcg cagaacacgg
     4801 tcagctcgcc ggtgtcggcg atgtacggca cctgcaggta tcccgagaac ttcacgtggc
     4861 cggtgatgta gcacatgccg ccgagcgcca ccgccgcgaa cgcagtgagc cccgccgcca
     4921 ggccgtccag accgtcggcc aggttcacgg cgttcgacgc gccggtcacg acgaggacca
     4981 cgaaggggag gtagagccag cccaggtgga tcaccgtctg cttgaggaac gggatgttgg
     5041 tctcgccggg gttgaggatc ccgggcgacg ggtagaagta gaccgcgaag aagatcccga
     5101 tcccgagaag gatctgcccc gccagcttgt accggccgag gagtcccttc cggtagtgct
     5161 tcacgacgcg caggtagtcg tcgatgaagc cgaccgtgcc catccagagc gtcgcgacga
     5221 gcgcgatcag cacgttccgg ttgccgagat tcccccagag gagcgtcgag accaggatcg
     5281 cgatcacgat caggatcccg cccatggtcg gcgtgcccgc cttgggaaga tggctctgcg
     5341 gcccgtcgtc gcgcaccttc tgtccgatct tgagctcgcg cagcttcttg atcaccggag
     5401 gcccgaggac gagcgcgatc acgagcgcgg tcgcgagcgc gtaggccgag cggaacgtga
     5461 tgtagcggaa aacgttcagc ggcgtgaagt agacgtggag cggatagagg aagtgataga
     5521 gcatcaggcc cgccctgccg gcgccggcga cgccgcgcca tgccccgttc cggaggcgcc
     5581 catgagcgcc tccaccaccc gctcgagccg catcccgcgc gacgccttca cgagcacgag
     5641 atccccggcg cgcgcgtccc gcgccaccga ctgggccatc gcgggcacgt cgtcgaagcg
     5701 gcggatgcgc gcggcatcca cccccgccgc gcgggcgccg cgctcgagat ccgccgcgtg
     5761 tccgccggcc gcgtagagcc aggcgccctt gggcaccgcg cgtccgacgt cttcgtgcag
     5821 ccgcgccgct tcaggaccca gctccagcat gtcgccgagc accgcgcggc gagcgccgcg
     5881 ggcgtccatc gagccgagca gggcgagcgc cgccttcatc gacgcggggt tcgcgttgta
     5941 cgcatcgtcg atcacgttca cgccgcccgg cgtcgtcacg cgctcgagcc ggtgcttggc
     6001 cgggcggagc gcgccgagcg cgcgcgccgc ctcgccgcgc ggaatgccga agcggtcgcc
     6061 gaccaggatc gcggcgagcg cgttcctcac gttgtggcgt ccgaggagtg ccagggtgac
     6121 ctccgttccg tcgggcagga tgaaccgggt gccgccgtcg ccgctctcca ccaccgagtc
     6181 cggcctcaag tcggcgtcct ggttgtcgag ggcgtagccg cgcaccgtca ccggacggcc
     6241 gcgattcatc gcccagagta gcgcgtcgtc ggcgttcacg accaggagtc cgtccgggcg
     6301 gagcgcttcg gcgagctcgc tcttggcccg cgcgatctct tcgaccgagc cgagctgctc
     6361 gagatgcgcc gcggaggcgt tcgtgatcac gcccacctcg gggctcgcga gcgacgcgag
     6421 gagcgcgatc tcgccgcgcg cgctcatccc catctcgatc acgagaacct gatgctccgg
     6481 ggtcaagtgg gacagcgtga gcggcacgcc gatgtgattg ttgagattcc ccacgttccg
     6541 gtgcacgtgg agccgcgcgc cgagcacggc ggcgatcatc tccttcgtgg tcgtcttgcc
     6601 gttcgatccc gtcacggcca cggtgggcac gcccgcacgc tcgcggcaat gccggccgat
     6661 cgcctggagc gccgccagtg cgccctcgac ctcgatcacc ggcttcgacg acggggccga
     6721 gcccgagctc cacgacgcac cgcgcgcgac gaggctcccc gcggcgcgcc cctcgagcgc
     6781 ggacgcgacg aactcgtggc cgtccacgtt cgatcccgga aggggcacga agagctcccc
     6841 gtcctggatc gtccggctgt cgatcgcgca cccccggaac gtgagtcccg ttccggccgc
     6901 gccccgcttc agagtgccgc cggtcgcggc gagaacgtcc tccaccgtca tccagggaat
     6961 cccggccaag cggctcacgc gcgatactcc cgcgtctcga gcgccttgcg cgcgacctcc
     7021 cggtcgtcgt acggaatcgt gcgcgagccg aggatctgat acgtctcgtg tcccttgccc
     7081 gcgatcagga gcgcgtcccc atcggcgcac gcctggatcg cggccccgat cgcctccgtg
     7141 cgatccacga tcgtgtgcac gtgggcggca ccgcgcacgc cggcctcgat ctcggccagg
     7201 atcgcgcgcg gatcctcgct cctcggattg tcggacgtca ggaagacctc gtccgcgtgc
     7261 tccgcggcga cccggcccat caagggccgc ttgccgcggt ccctgtcgcc gccgcatccg
     7321 aagacgcaga ggaggcgctt gggcccggtc gcgcggaccg cgtcgagcgc gcgcagcagc
     7381 gcgtccggcg tgtgcgcgta gtcgacgaga accaggaagg gctgccccgc ctcgacgggc
     7441 tcgagccggc ccggcaccga cgcgaccgat tcgagcccgg ccgtgatcgc ggtgaccgga
     7501 acgccgacgg cgagccccgc ggcgaaggcc gcgagcgcgt tcatcacgtt gaacgcgcca
     7561 cggagccgga gggcgaccga agagcttccc tgcgggcttc ggatccggag tctcgttcca
     7621 gatcgctccc accggatatc ttccgccgtc acctccgctc cgggctcgag gccgaatcca
     7681 agcatggggg ggtctccatg ctcccggatc cagcgccctt cgggatcgtc cgtgttgacg
     7741 gccccggtga acgccttcga gctatccccg cgcgtctccg cgcggaagag ccgcgccttg
     7801 gcgtcccggt aggcctccat ggtcccgtgg aaatccagat ggtcctgcgt caggttcgtg
     7861 aacacgcccg cgtcgaacgc gaccccgtac gtccggtcga gcgcgagcgc gtgcgaggag
     7921 acttccatcg cgacggcggt cgcgccgcgg tcgcgaaatc ggcgcaggag ccgcgcgaga
     7981 tcgggcgcct cgggggtcgt gtgcggggcg ggctcggtct ggtcgccgaa gcgatagccg
     8041 accgtgccga tgacgccgac gcgctcgccg cgcgcctcga gctcggccgc gatcaagtgc
     8101 gtcgtcgtcg tcttgccgtt ggtgccggtc acgccgatca tcacgagctc gcgtgacgga
     8161 tggccggtct cctcggcggc caggagcgcg agcgcgcgcc tcgagtccgt gacccggatc
     8221 tgcggcaggt cgagtcccgg cgtcacggtc tcgacgaccg cggccaccgc tccctggtcg
     8281 cgcgcctgtc tcaagtgcgc gtggccgtcc gtcttctgtc cgcgaatggc cacgaagatg
     8341 tctcccgatc cgaccgcgcg cgagtcgtac tggacgcgct cccagacgcc gcgcggagat
     8401 ccatgcacct cgaccgcacc cacgatccgg gcgatctggg cggcgtgccg ggcggacgct
     8461 tcgatcaggc gcctcggggc gagaggacca gcgtgcatcg ggtccccttc ttgacggtct
     8521 tgcccgcttc gagcgactgg cggacgacgg tgccgcttcc ctcgacgcgc gccacctcga
     8581 tcgcgctcgc ggtgagccgc gcgagcgcgt ctcgaagcgc gagcccgcgg aggtcgggca
     8641 cggtggtctc gccgtcccct cccgtcgcga gcgcgagctc gatcaccgca ccggatccga
     8701 tctccgttcc gggagccggg gtctgcgcca tcacgcggcc gccgacggcg ccgccggagg
     8761 cgacgcgaag tccgtcgccc gcccgcgcga ggacctcgcg cgcccggtcg agctcgagga
     8821 gccgcacgtc cggcaccgcc atgcgcgcct tgggctcgtc ctcaccgcgc accaccgagg
     8881 acggagggag cgcgatcggg ccgcgtccct ggagcgccca cgcggcgagg atgtcgcgga
     8941 acacaggggc ggcgacggag ccgccgtagt agagaccgcc gcgcggctcg tcgatcacga
     9001 tgagcccgac gacggatgga ttctcatagg gggccatgcc gacgaacgag gagatgtagc
     9061 tgccggcgcc gtagccgcgc ccgttctcga gcgccttctg cgccgtgccg gtcttccccg
     9121 ccacctcgca ccacggaagc gccgcttcgg tcgccgtgcc cgacacgacc gcgtcgcgca
     9181 ggaaggaacg gaacgtgcgc gcggtttcgg acgagacgac ccgccgcacg tgctcgaccg
     9241 gaacctcgcg gatcggcgcg ccctgatcgt ccaccaccgt cgagacgacg cgcgggcgca
     9301 agagctcgcc gccgttcgcg atcgcgcagt aggccatcag gatctggagc ggcgtcacga
     9361 gcacttcctg gccgatcgag atggtcgcga gcgagcgccc ggaccagcgg tcgggatggc
     9421 gaatctgccc gggcgcctcg cccggcagca tgatgcgcgt gggctgcccg aagccgaaga
     9481 gccgcgcgta gccgtagagc gtcttcgcgc cgaccttcgt tccgatgtgc gcgtagcaga
     9541 cgttgctcga gagcgccgtg gcgtcgcgga aggtgtacca gcgggtctcg ggatgcgagt
     9601 cgtggatcgt gaagcctccg acgtccgctt cccccttgtc cacgtagaag gaatcggacg
     9661 gctggaggag ccgctcctcg agcgcggcgc agccggcgag gaccttgaac gtggagccgg
     9721 gctcgtactg gtcggcgatg acgcggttcc gattgtggtc cgagtccgtg cgtcccgcgg
     9781 cgtccaccgt caccatcgcc aggatgtcgc cggtccgcgg atcggcgaag acggccatgc
     9841 cgcccttggc gccgttcttc tccaccgcct tcttgagctc gatcgcggcg atcgactgcg
     9901 cgtcgagatc gaccgtgctg acgatgctct cgccggactc gggaagcttc acgagactcc
     9961 gcggcagctc gtacgcggcg ccgcgggcgt cgcagaagag cgtcgcccac ccgctcttcc
    10021 ccttgagctc gcggtcgtac acgcgctcca gcccctcgag cccgtcgccc tcgctcccga
    10081 ccacgcccac caccgagcgc gcgatcgtct cgagcggata gacgcgcccc ggcacggctt
    10141 cgacgcgcaa cccctcggga agcgtcgtgg cctggagcgc gagctcctgc tgcggcgaga
    10201 gctgcgagga gaggcggatg tacgccgcgt ggcggctccg tccggccttg aagcggctca
    10261 cggcgttggc ccccgccgct ttcgcgagga aggtctggat ggctcgcgcg ttcttcacgt
    10321 cgcgaggacg cacggagagc cggctcaggg cgacgttgtc ggcgagggag cggagatggc
    10381 ggtcgaagat gcgcccgcgc tcgggctcga ggaggacgcg ccgcgtctgc tgctgccgcg
    10441 ccgccttggc gaagctctcg tgctggacca cctggatctg gacgagccgg gtgaggagca
    10501 tgaggaacgc cgcgaacccg acgaccgcga gaaggcggag acgccgggcg tcgttcggaa
    10561 tcacggaccc acctgcgcgc gcaccggcac cgcgtccaca cccggaaccg cgggcgtggc
    10621 gttgcgacgc ttcaggccct ggaaccagga gctcacgcgc gcggcgacgc cgcccggcat
    10681 ggcgtgcggg agcgaatctt tcaggtcgat gtccacgatg tgcgcgggat cgggagcgac
    10741 gaacccgagt gcgcggagcg agcggggatc cacgccggag aggagcgacg actgctcgct
    10801 cacctcggac gagagctgga gcctgttctc gagggcgcgc acctcgcggc gcaggcggtc
    10861 gcgatccatc gcggtcgcgt gcgcgacggt gttctcccac acccgcgcga agagaaggag
    10921 gagcgccgcg aggccgatca ggagcgtctt gccatagcgc acgcggcgtc cgcgaacggt
    10981 gtagcggcga agataggcct ggtcggggcg ctcctttgcg cccggcgtca cgtctcgtgg
    11041 taggccgcgc tccagggtga ggctcaccgg cggttctcct ttcgctccgc aacccgaagg
    11101 cgcgcactcg cggcgcgggg gttgacggcg atctcctcgt cggtggggcg gatcggcttc
    11161 cgcgtgaggg ggatccacgg ggtttcggcc ggctcctccc agacgtcgag agggcgagga
    11221 gggcggaatg cggtcttcac gatccggtcc tccagggaat gataggcgat cacgcagaga
    11281 cggcccttgg tcctcgtgcg ccgcgcggcc gtggcaagga gcgcttcgag ctcctccatc
    11341 tcgtggttca cggcgacgcg gagggcctgg aacgcttgcg ccaggcggcg gggacgctgg
    11401 gggccgggat agatgggagc gagcgcgtcg cgcagggccc cggtcgtgag gggttgcacg
    11461 gcgcgctcgg cacggcggat cgcgcgcacg gcggaccgtg cgcgcggcag ctcgccatag
    11521 gatccgagga tccgctcgag cgctcgatcg tcgacgccgc gaatcagcgc atcggccgac
    11581 atcggccggg acggatcgag tcgcatgtcg agcggtccct cggtggcgaa cgagagtcca
    11641 cgggcgggat cgtccagctg gtccgagctc aggcccagat cggcgaggat cccgtcgact
    11701 tccgtgattc cgatttgatc gagtcgggaa tcgagatcgg aaaaacgccc ccgaacgggg
    11761 gtgaaacggg gtggcggcgc cggcgggttc gcgctcgcgc gctcaaccgc cgccggatcg
    11821 cagtccaacg cgatcaccct tcctcctcct cccaacgccg ccaccagttc actcgagtgg
    11881 cccccgaggc ccagggtcac gtcgacgtag acgccctcgg gatcggtgac cagccaccgg
    11941 attgcttcct gcttcaagac cgggacgtgt cccggcatct ttctctaggc caccatcccg
    12001 tcccacgcct cggcatggga catccttccg tcctcgaggc tcggagccgg cccctcggcc
    12061 gcgccccatt cgagccgcgc cagcgcgggg ccctgccccg ccacctggaa gagcgcccgg
    12121 aggtacgggc tcagccccac catccagagc gacgccccgc gctcgcgctg gcgtccgaga
    12181 cgcgacacga actcggggag cgcccggtag tcgaggtgct cgacctcatc gaagtcgagg
    12241 cacgcgagat cgcgcggcga gcggcagcgc tcccggagcg tttcgaccac gcggtccaga
    12301 tcgcgggcgc cgagcacgcc atagagccgg agccggaccg gaccgcccgc gcgcgacagc
    12361 tcccactggc acgtggggcg gtcgatcttg agcgcggtgc gcccgttcgg gatcgcgatc
    12421 acgggaggat ctccccggcg atgtcctcgt acgtgcgatt ggaggacgcc aggtactgct
    12481 cgaacacctt gggatcccag atctcgatgt gatcgaggac gccgttcacg agcacgtcct
    12541 tcgtgatctt cgcgtgctcg agcaggcgcg gcgggatcgc gacgcgcccc tgcgcgtcga
    12601 gcgtctcgtc gcgcgagtcg aggaggaaca ggcgctggaa gcgccgcgcc gcctcgtcgc
    12661 cgaaggagcg cgagcggagc cgctcctcgt agctcttgaa ctcgtcggag ggaaagagcg
    12721 acacgcacgc gtcgaaaccg cggaggacga cgaacgagcc ttcggcgtcg ccggagagac
    12781 tgcgccggaa gcgggcgggg atgctcacgc ggcccttgtg gtccacggag tgctgatagc
    12841 tcccccggaa cgtcgccatt gccctcgctc tcgatcgctg ttgttgccgg aaggaagagg
    12901 tttcatggct ccccacttca ccccacaatt tgccacgcgg cgcatcctag aagggcgcgg
    12961 cggggggtgt caacaaacaa tcgtcacttt tctgaaattt tatttggttg gggcccagag
    13021 ccttggcgct gggtgggatc ggggggggtg cggtgggtaa aaattagccc gcggcgggct
    13081 cggcgaggcg ttcgaggatg gttgcggccc ggccggccat ggcctttcgc tcccatccgg
    13141 cggccagggc ggccgggggg cggaccgccg aatcctggcc ggcccggaag gaccggagga
    13201 gactctcgac ggcccggctg gtcgagccga cgtctccgga ccggaccacc gtcccggcgc
    13261 cggcctcgag gatcagatcc gcggccggcc ccgggtccag gacgcccagg atgggacggc
    13321 cgctcgtcag gtactcgtag agcttcccgg tgtagaggag cgaatcggcg tcgcctccgt
    13381 gtcccaggag cacgagggcg tacgcgcggc gcatggcctg gagcgcttcc agatgcggtc
    13441 gcgacggctc cagctccacg atgccgtcga gaccgcgcgc ggcccactcg tccggccgga
    13501 gctcctcgtt tccgcccagg aagcgaaggc gcagctgatc gcgcgcgccc ggatcggcct
    13561 cgatcacgcg cgtgagtgcg tcgaggagcg tgcgcaccgt ggggcgatgc gcgagctgcc
    13621 ccgcatggag gagcacgcag cgtccctcgt ctccctcgcc ggctcgaggc ggaagctccg
    13681 ccgcccgcct ccagtcgtcc gaatcgacgc cgttcgggag cgtgaggaac ttctcgcccg
    13741 ggagatcgcg gtagcgcttc cggtagagcg cggtcatcgg ctcgctcacc agcgtcacgc
    13801 ggtccgccgc ggtcacgacc gcgcgctcga gctccttgtg acgcgcgtcg tgccacgccg
    13861 tcgggggcgc atacgtgacg cgtcccaccc agggatcgcg aaaatccgcg acccatggaa
    13921 gtccgaagcg ccgcttcacc gcgaggccgg ccagatgcgc gctctccggc gaggaggtcg
    13981 tccagagcgc gtcgatccct cccgccgcga tgcggcgcac ggccgcgcgc tccgcctctc
    14041 gcgcccacgc gcggtaccca tcgggaagga ggagccagga ctgcgcgcgc cgcagcctct
    14101 ggaacgcgcg cgcgtcccgc gctccctgcc gcctctcgat gccgccggcg ttcgcgagca
    14161 ggcgcacgag ccggagcgag gtgagcccgc ccacgcggat gacctcggcc gtgcgcggca
    14221 cctccgcctc gagcgacgga tcgaggatcc agtagtcggc gtcgcgcgtg gtgaccacgg
    14281 tcgacgccca cccctcgggc tcgaggtagc gcacgagctt gagcgtccgc tggcagccgc
    14341 ctcctccgag cggcgggaag aagtaggcga tcgcgaggag ccgcttcacg acaccgcccg
    14401 cgcgggtgag gcgccggcca ggtcgaggag ctgctgctcg atcaggtcca cggtgtccgc
    14461 aagattcccc cgctcgcgag cgacgctcgc ggcgcgctcg gacatcgctt ccgcggaggc
    14521 cgcgccttgc gccacctccg cgagcgccgc ggcgaccgct tcctcgtccc ccactggaac
    14581 gagcagcccc tccttgcggt gctgcaccca ctcgagattg ccctcgatgt cggtgacgac
    14641 cggcacgagc cccgccgcca tcgcctcgag gagcgactgc gacgtggaat cggagcgcga
    14701 gagggacacg tacgcgtggt ggctccggta gagcgcggtg atccgggggc ggtccaccca
    14761 cccggtccac gtgatccgat ccgcgacctt ctcgcgcttg gcgagacgct cgagtcccat
    14821 gcgaagcggc cccgatccgg cgatggtcgc ttcgaacgag acgccgcgcc tggcgagcgc
    14881 gccgagcgcg cggacgaacg tctcggggcc atagagatct tcgagctgcc gcgtccagag
    14941 gatccgaagc gctggacttc gctcgcggcg cgcgctccac gcctcggacg gagcgaggga
    15001 cggaacgtcg acgccccacg cgcgcgtcca gatccggtcg cgcggagcgc ccagctcctc
    15061 ggccgcgcgc gcgagcacgg ccgcatccac gtggatccga tccgcgcgcc ggagcgtcca
    15121 ccgcgcccgc gcgcgatgga ggggcgtcct ccaggcgttc acgaggagat cggatcccca
    15181 gcagacgagc atccaggggc ggacgccggc gagggcggcg aggaagccgt agttcggaag
    15241 gaagtgcgcg accgtgacgt cgggcttgaa gcggaggatc tcctctctca ccgcgccgcg
    15301 cgcgctcgcg tactggaggg cacgcaccgg cacccgcgct cccaccacgc gcacaggcac
    15361 cggaccgggc gcgccgggct cggccgacag gaggcggatc gcgtgtccgc gcgcggcgag
    15421 cgcgcgggcc caaagggcgg tgtgggtcga cggcgcgtgc gcgaggagga ggatgcgcac
    15481 gccgctacgg acgaagccgc ctgtggatcg cgctcctccg cgtcagcgtc cagacggggc
    15541 gcggcctcgc gccccaaccc tccttgaagc ggatcagccc cgcggcgtcg gacggcgaag
    15601 cgccgaagtt gtactcggtg tatcccgcct ggcagagcgt ctcgaggagg ctcgccacga
    15661 ggaaattgtt cggccggagc ggccaggacg actcgcgcga gaccgggagc cagctcgtcg
    15721 ccacgccttg atggaagaga tggaggctcc ccgccaccgc cttgccctcg tgctcgacca
    15781 ggtggtagcg cgcgagcccc gcggtcacga gatccccctg gattcgctcg tagagcgcgc
    15841 gcggcttggg ctttccgccg cgccggacca ccgtctcctc ggcgatctcg atgaactgcg
    15901 cgacgtccgc cgcggactcg gcggcgcgga tcgagagtcc gctctcgtcc gattggcgca
    15961 ccagtctcct ggtggacgga gagaagcgat ccgcgacgcg ctcgtagtcg ccctcgagcg
    16021 cgagcacgtg cgtgaacccc tcgtctggcg gcgattcctc ggggaattcc tcgggccgtc
    16081 cgccgtacca ggtgagctgg gctcggagca cgcgtcgtcg cgcggcgtgc gcgaggaacg
    16141 tctcgatgag cgcgcggcgg gcgcgaggtg cgtccaagtg cccttcgcgc acgatcgggc
    16201 cgccgtacgt ggcgtacggc atcgcgtcga gggattcgaa ccagccgtgg cggcgcgcga
    16261 tccagccgag cgcgcccacg tagcgtcctc cctcctcgag gatcgcggcc tcccacttcg
    16321 tcgaggactt gaggccggtc cagaggcgcg cccacgcggc ggtgtgaaac acgctcccgc
    16381 ccggcgacgc ctggacgaga tcgtcccaag cttcgggatt gggcgccatg atctcggccg
    16441 gaagcgcgtc cgatttcaac gcaccaccgc aacgcgcagc atccgatgct cctcgcggtc
    16501 ccggatgacg acgacgaaga cgcccgaccg ggcgcgcgtg ccgtcggcga ggaggagatc
    16561 ccagaccacg tcgtgcgcgg gatcgcagcg gaagcgccgc acgatggctc cggtcacgtc
    16621 gcgaatctcc cccgtcacct cgcccgtgat gccgccgatc cgaagcgcgg tggtctcggg
    16681 cccgatcggg ctcggataga cgtagctctg cgacgtgaac gtcgtcgagc ccgccttcgg
    16741 caccacgacc tcggagatgc cggacgtcgt gcccacccag agggtgcgct tggccgcgtc
    16801 ccacgcgagc gcgcgcacgt cgtcccccgc gagcccctcc gcggtcgacc agtgctccac
    16861 gaaggtcgag cccgaggcca cgcgggcgag tcccgaggtc gtcgcgaccc agaggttccc
    16921 ctcggagtcg agctcgagat cgcgaaccgc cgtcgacggt agcgccgcgt tgattgcggt
    16981 cgtcgtgccg ggaacgacga cgtcgttcac gatccggacc acccccgcga ccgttccgac
    17041 ccagccggat gacgcgccgt cggtctcgac cgcggtcgcc tgcacggaag gaaagccggt
    17101 ggcgagatgc ttccagacgt cgtccgaatg atcggtgagg gttcctcgtc cgtcccagat
    17161 gtcgaggccg gtggccgcgt gcgcgatcca cccgcgcccc gtcgtgtcga acgagattcc
    17221 gcggaggttg ttgctcgcga gcccggttcc gatcgccgag ccctgcgtgt tgagtggcgt
    17281 gagggagtcg acgacggcga gcaccgtggg atcgtagacg tagactccat tcccgtgctc
    17341 cacgcttcct tcgtacatga gcgcgtcggg accgcgcgtg atcacgaaga tgttggtgct
    17401 ccctggtgcg ttccacacgc cggtgtcggg atcgtagcga tcggtccgcg gcagtggatc
    17461 gaccgtggag cagcagtgcc ccatccagat cgctcccagc ggatcttccg cgagcccgaa
    17521 cacgctggtc gactggagcg cgccgccggt ggtcgcgggc gagaggagcg accaggtcgt
    17581 gccgtcgtag tggaggacgt tcccgaggct ccctccggga ggcacggcgt tgccggtcgc
    17641 gaaccacgcg ccgcgcacgt ccacgagcgc gcgctccgcg ccgttcaccg ccgggccgtc
    17701 ggtcgggtac agcgtccatg tgtccgtccc cgcgtcgtag cgggcggtgc cccgatccga
    17761 ccctgcccag aggcttccgt ccggcgccgt gacgaacgag gacagccgca cgcccggcgg
    17821 gatggatccg aacgccgacc acgcgccgcc ggtgtagcga tacgccccct gcacggcgct
    17881 gccggagaga agccggacgc catccgtgaa gagagcgaga ctcgatccga tgtgcccgac
    17941 ggcgacgacg gtgaacgctc ctcccgcgta ccggcgcggg cccgccgccg tggccgccca
    18001 cagcgtatcc tgatgaactg cgaggtcgcg cgccgccacg gcgagcgtcg acgcgcggtt
    18061 ctgccacacg ccgctcgcga acgtggagag ccccgcggaa gttccgaccc agagcgtgtc
    18121 cgcgacttgc tggaaggcga ggacgtcgtc cgacacgagc cccccgccgg tgctcgcccg
    18181 cgagtcgctt cgcctgagag ttgtctgccc gcccgcactc tcggtgaaga gggccacccc
    18241 gccgctcgtc cccacccaca cgctgtcgcc gtgcacgtag agcgtctgca cgtgatcgct
    18301 cgggagcccg tcgaaggacg tgagggtgcg cttgaacccc ccacccggga cgagccgcgc
    18361 cacgcccttg tccgcggttc ccgcccagag gatcccgctc ggtccgaccg cgaccgcgag
    18421 cacctggttc gacgggagcc cgctgggcga cgccaggagc ttcgtccgca cctgggtcgc
    18481 gggatccacg aacaccaaac cgcccagcgt ggccgcgacg atcctcccct gccacacggc
    18541 catcgctccc acctggtcgg agtggacgtg gttggtgatg aagatcggtg tcgccgcgcc
    18601 ggcgggcgcg gcgagaagcc atccggcgag gagcaccaaa gctccgcgcg tgcgagcgtg
    18661 tcccctcacc gcttccgctc cgtcgcgcgc aggaacacgc gcaaatcctc ggcgatcgag
    18721 aagcgcgtga gatacgcgag ctccgactcg aggcgcgccc gccggtccac gcccgagccg
    18781 ttcgcggggg acacgacgcc ggggcggagc gactcgagcg cgagccgcgc ccagggcggc
    18841 actccggtcc agcggtccgc ttcgtaggtg ctccgtccga cccagctccg ggccccggtc
    18901 acgacctggc gccacgcttc gcccgcgccg agaccgtccc cgcgctccgc gagatacgag
    18961 cggatgcgac cccagcgcca gggggcgatc aggaacgaga gcaagagatc gctcgcccgc
    19021 ttccgcgcgc ggagcgagag gccgcggctc ggcgcatgga ggagaaccgc gtcgagcggc
    19081 ccgagacggc gcgattcccc cgcggccgcc gccaggtgcg cgaccgagcc cgcccaccag
    19141 agggccgcgc ccgagtcgcg gagcgcggcg cccatcgcga ggaggtatgg cacctcgtcc
    19201 ggctccggca cgagacagac gatctgcacc cgctccccaa ggacgaggga gcggatgcgc
    19261 gcggccgcgg cctccggggg ctcgtccgga cccgacgagg ccagcgcgtg gagcggctcg
    19321 aagccctcgc ggcgcgagcc ctcgagcgcc gcgcgcgccc gctcgacctc ggcgcccgag
    19381 cccaggagga gcacccggcg aaacgcaaag ccctgcttcc gagcgccggc ggccgcgctc
    19441 gcgagcgctc cgcgcgccac gtagaggaag agaaagagga gcggccccac gagcagcacg
    19501 aggaagcgcg aatagcgagg catctggaag aggaacgtcg cggccatgag gaggagcacc
    19561 gcctggacga ccgacttccc cacgaggaag gcgcgctcga tccggtcggt gaagtcggtc
    19621 cgccggtaga ggccgttcat cgcgagcgcg gcgatcgtga cgccgaccgt gaagtagagg
    19681 aggcccgcgt agctctccgc cggaaaaacc gggcgatcga accacggaag agcgcgccct
    19741 gccggacggc tttcccgatt ccgccgcgct cgaggcggcg ctcgacgccg agcggcggag
    19801 aacggagcag gttccacccg ctttttcggc gatctcactc gacggacagc gctcccaccg
    19861 gcgggcgcgg cgcggcgagg aggtcgtcct tccccctcgg cccgtcctgg tgaaggcgct
    19921 ccgcctgaac gcgtgccggc aagccgcggg caacgactcc gaggcctctg cgatcctgga
    19981 gattgcggtc tcgaagggct actacgtacg ctcgctggcg cgagacctcg gcgcggcgct
    20041 gggtgtgccg gcgcatctgt cgcgcctcga gcggacggca agcg
//
LOCUS       DASTKC010000119         5027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_323378, whole genome shotgun sequence.
ACCESSION   DASTKC010000119 DASTKC010000000
VERSION     DASTKC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323378"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFR25_04970"
     CDS             <1..529
                     /locus_tag="VFR25_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940432.1"
                     /translation="QAFYDGQIEVAQVTANQFARTYVRNLKVNEALEMVLLAKNCRDF
                     DYQPLRAYGRVLSLRATGHPDSAASVAKATLRDWPGSAVRYHLRYQLADLARERGDHT
                     EAVAQALAVADSTSKSRLAPAALKLAGDETIAMGQGQEKALKIYQELLERYPDSPLAP
                     GVRSQILDMRKKLQL"
     gene            622..1800
                     /locus_tag="VFR25_04975"
     CDS             622..1800
                     /locus_tag="VFR25_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HET9940433.1"
                     /translation="MDQGQTDHLRAYGLTYWALSHGLRGEWLLNYRGGSFLLPDNAQL
                     QSEAVVRGVSMEVIDEAAVARIMAEIADNNMESIKLETTPRVAVYVPPSSPPWDDAVQ
                     LALDYAQIPYTKLWDAEVLDGKLAEYDWLHLHHEDFTGQYGKFFAAFAATDWYRRQVA
                     ENEAMAKTHGFAKVSDLKKAIARTIKSYVANGGFLFAMCSATDTFDIALASEGVDIVG
                     PEYDHDGFDPNANSKLDFSQCLAFQNFELEMNPLVYEFSNIDTTTKASTRGQRNDFFT
                     LFDFSAKQDPVPSMLVQDHVANVPGFMGQTTGFEKTMLKPGVTILAETPGADDVKYLH
                     GHYGKGTFTFYGGHDPEDYQHAVGDPPTDLALHKHSPGYRLILNNVLFPAAEKKEKKT
                     "
     gene            1866..2915
                     /locus_tag="VFR25_04980"
     CDS             1866..2915
                     /locus_tag="VFR25_04980"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9940434.1"
                     /translation="MSEHDKNNPEPLKPLYDPVAGHAPAALVTGPTGAIGRVVCRELV
                     QNGYRVLGLARNSDQSARLPYAVVPVLGDIRDPGRWEAAITRADVVIHAAIPPAPKGP
                     KDRAYAAREAEELAAIAAGIATAVRKKKKRLIATSSAILFEPNKDGWVTETSGLSEGK
                     GYGIRQRQIYRAIQGQRKKGLKAIVMCPAFVYGPGGWFEKSMLEPMSRGESRMIGEGN
                     QTMHYVEGSDVGRAYRLAIEHGVDGDDYLIGDDKPSTHGAFTRLVAREMGAPAPVAVP
                     EEDLIPILGDWGLEAMTTCPKIDSTRARERLGWSPEYKTTEEGVPVVVRDYKWRRERE
                     PELEPFRLVQHRSWT"
     gene            complement(2938..4068)
                     /gene="cax"
                     /locus_tag="VFR25_04985"
     CDS             complement(2938..4068)
                     /gene="cax"
                     /locus_tag="VFR25_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009344025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HET9940435.1"
                     /translation="MEPAAGHGGVGARIFAPENALQLLLVFIPIAVLAERLHWGPLAV
                     FGCAGLAIVPLAGLMGRATEQLAARLGAGVGGLLNATFGNAAELIIAFAALQRGLYDV
                     VKASLTGSIIGNVLLVLGVAIFAGGLKRQSQTFDRAAAAVNSTLLALAATGLVVPAIF
                     HPIAMNAVASNRVTPEHEAILETGLSLEIAVVLFIAYGAYLLFSLGTHRHLYKGGEHE
                     AHGPAVPTWRSVVLLLVATALVAWMSEILVGAVEEASHVLGLTEIFVGVIVVAVIGNA
                     AEHSTAVLVAMRNQMDLALQIATGSSIQIALFVAPVLVFASHFMKHGPMNLRFTPFEV
                     LSVLLATAVVNLVAQDGESNWLEGALMVAVYVILGIAFYFIP"
     gene            4126..>5027
                     /locus_tag="VFR25_04990"
     CDS             4126..>5027
                     /locus_tag="VFR25_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940436.1"
                     /translation="MTGTLGTVVSFLIGLSALATPVGIALAAESDSVTVTAGAHYGRS
                     GFHRFFFGSDYRKVWTTPIRIPVLDMSREAGGLRPVRRVGGRQTQALALAGADGRSYT
                     FRGVAKDVSGLIDEGLRGTIVESILKDQMAAQHPAGELIAGSLAKAAGIRTQEWVLVA
                     LPDDPALGEFRDEFKGAIGFFGEYPTAPTGERPGTFGLKEIVGYKEIYLKLETDPSTH
                     IDAGEFLYARLFDLAIGDWDRHRRQWRWARFEGDPLWHPIPEDRDQAFSRYEGLFLGI
                     GRRTRDQRFQKYDDHYKSVSGLMW"
BASE COUNT          896 a         1695 c         1689 g          747 t
ORIGIN      
        1 ccaggcgttc tacgacgggc agatcgaggt ggcgcaggtg accgcgaacc agttcgcgcg
       61 gacgtatgtg cggaacctca aggtgaacga ggcgctcgag atggtgctcc tcgcgaagaa
      121 ttgccgcgat ttcgactacc agccgctccg tgcgtacggc cgcgtgcttt cgctccgcgc
      181 gacggggcat cccgattccg cggcgtcggt ggccaaggcg acacttcgag attggccggg
      241 ctccgcggtg cgctaccacc tccgctatca gctcgcggat ctcgcgcgcg agcgcggcga
      301 tcacacggaa gcggtggccc aggcgctggc ggtggccgac tccacatcga agagccggct
      361 cgcgccggcg gccctcaagc tcgccgggga cgagacgatc gcgatggggc aggggcagga
      421 gaaggcgctc aagatctacc aggagctgct ggaacgttac cccgactcgc cgctcgcgcc
      481 gggcgtccgg tcgcagatcc tcgacatgag aaagaagctc cagctatgaa gcgattcctc
      541 gcgcgtgcgt cgctcctgcc gctcctcgtc gctttcgcga tcctggggcc tgggtcggcg
      601 tgggcgcgca tcctcgtgcc gatggatcag gggcagaccg accacctccg cgcgtacggt
      661 ctcacgtact gggcgctgag ccacggcctc cgcggcgagt ggctcctcaa ctaccgcggc
      721 ggatcgttcc tcctgccgga caacgcgcag ctccagtcgg aggcggtcgt gcgcggcgtg
      781 agcatggagg tgatcgacga ggcggccgtg gcgcggatca tggccgagat cgcggacaac
      841 aacatggagt cgatcaagct cgagaccacc ccccgagtcg cggtctacgt gccgccgagc
      901 tcgccgccgt gggacgacgc ggtgcagctc gcgctcgact acgcgcagat cccgtacacg
      961 aagctatggg acgccgaggt gctggacggg aagctcgccg agtacgactg gctccatctc
     1021 caccacgaag acttcaccgg gcagtacggg aagttcttcg ccgccttcgc ggcgaccgac
     1081 tggtatcgcc gccaggtggc cgagaacgag gcgatggcga agacccacgg cttcgccaag
     1141 gtctccgacc tcaagaaggc gatcgcgcgc accatcaaga gctacgtggc gaatggcggg
     1201 ttcctcttcg cgatgtgctc ggccacggac acgttcgaca tcgcgctcgc gtccgagggg
     1261 gtggacatcg tcggaccgga gtacgaccac gacggcttcg acccgaatgc caactccaag
     1321 ctcgatttct cgcagtgcct cgccttccag aacttcgagc tggagatgaa cccgctggtc
     1381 tacgagttct cgaacatcga cacgacgacg aaagcttcga cgcgcggcca gcggaacgac
     1441 ttcttcacgc tcttcgactt ctcggccaag caggatccgg tgccgtccat gctggtgcag
     1501 gaccacgtcg cgaacgtccc cggcttcatg gggcagacga ccggattcga gaagaccatg
     1561 ctgaagcccg gcgtcaccat cctcgccgag acgcccggag cggacgacgt gaagtacctg
     1621 cacggccact acgggaaggg aacgttcacg ttctacggag gccacgatcc cgaggactac
     1681 cagcacgcgg tcggcgatcc tcccaccgac ctcgccctcc acaagcactc gcccggctac
     1741 cggctcatcc tgaacaacgt cctcttcccc gccgcggaga agaaggaaaa gaagacctga
     1801 gcgtcgcggg ctttagcccg tgaccctctt gagccgcccg cgtcctcgcg gtacactgcc
     1861 cggccatgtc cgagcacgac aagaacaacc ccgagcccct caagcccctt tacgatcccg
     1921 tcgccggcca cgcgcctgcg gcgctcgtga ccggaccgac cggcgccatc ggccgcgtcg
     1981 tctgccgcga gctcgttcag aacggctatc gcgtgctcgg gctggcccgg aactccgacc
     2041 agtcggcgcg ccttccgtac gcggtcgtcc ccgtgctcgg cgacatccgg gatccggggc
     2101 gctgggaggc ggcgatcacc cgcgcggacg tcgtgatcca cgcggccatt ccgcccgctc
     2161 ccaagggacc caaggatcgc gcgtacgccg cgcgcgaagc cgaggagctg gccgcgatcg
     2221 ccgcaggcat cgcgacggcc gtccggaaga agaagaagcg gctcatcgcc acctccagtg
     2281 cgatcctgtt cgagccgaac aaggacggct gggtgacgga gacgagcggc ctttccgagg
     2341 ggaagggcta cggcatccgc cagcgccaga tctatcgggc gatccagggc cagcggaaga
     2401 aggggctcaa ggcgatcgtg atgtgccccg ctttcgtcta cgggccgggc ggatggttcg
     2461 agaagtcgat gctcgagccg atgagccgcg gcgaatcgcg gatgatcggc gagggcaatc
     2521 agacgatgca ctatgtcgaa gggagcgacg tcggccgcgc ctaccggctc gccatcgagc
     2581 acggggtgga tggcgacgat tacctgatcg gcgacgacaa gccgagcacg cacggagcct
     2641 tcacgcggct cgtggcccgc gagatgggcg cgccggcgcc ggtcgccgtg cccgaggaag
     2701 atctcatccc gatcctgggg gactggggcc tcgaggcgat gaccacctgc cccaagatcg
     2761 actccacgcg tgcgcgcgag cgcctgggct ggagcccaga gtacaagacg accgaggaag
     2821 gcgtgccggt cgtcgtgcgc gactacaaat ggcgccgcga gagggagccg gagctcgagc
     2881 ccttccgcct ggtccagcac cgaagctgga cgtagcaccc cgggggcgcg cccgcgctca
     2941 tgggatgaag tagaacgcga ttcccaggat cacgtagacc gccaccatca gcgccccttc
     3001 cagccagttc gattcgccgt cctgtgccac cagattcacc accgccgtcg cgaggagcac
     3061 ggagagcacc tcgaacggcg tgaatcgcag gttcatgggt ccgtgcttca tgaagtggga
     3121 cgcgaacacg agcaccggag cgacgaagag cgcgatctgg atgctcgagc ctgtcgcgat
     3181 ctggagcgcc aagtccatct ggttgcgcat ggccacgagg acggcggtcg agtgctcggc
     3241 cgcgttcccg atcaccgcga ccacgatcac gccgacgaag atctccgtga gccccagaac
     3301 gtgcgacgcc tcctcgacgg cgccgaccag gatctcgctc atccacgcga ccagcgcggt
     3361 ggcgacgagg aggaggacca ccgagcgcca cgtgggcacg gccggcccgt gggcttcgtg
     3421 ctctcctcct ttgtagaggt gccggtgcgt gccgaggctg aagaggagat acgctccgta
     3481 cgcgatgaag agaaccacgg cgatctcgag cgagagcccg gtctcgagaa tggcctcgtg
     3541 ctcgggcgtc acgcgattcg aggcgaccgc gttcatcgcg atcgggtgaa agatcgcggg
     3601 aaccacgagg cccgtcgcag ccaaggcgag gagggtcgag ttcacagcgg cggccgcgcg
     3661 atcgaacgtc tgcgactgtc gcttcaaccc gcccgcgaag atggccaccc cgagcacgag
     3721 caggacgttc ccgatgatcg atccggtgag cgacgccttc acaacgtcgt agagtccgcg
     3781 ctggagcgcg gcgaacgcga tgatcagctc ggccgcgttc ccgaatgtgg cgttcaggag
     3841 cccgccgacg ccggcgccca gcctggccgc gagctgttcc gtggcgcggc ccatgagccc
     3901 ggcgagcgga acgatcgcga gcccggcgca cccgaacacc gcgagcggtc cccagtggag
     3961 gcgctccgcc aggaccgcga tcgggatgaa cacgagcagg agctggagcg cgttttcggg
     4021 agcgaagata cgcgcgccta cacccccatg ccctgcggct ggttccatgc gcgggtcagt
     4081 ataaccgcga tgtcaccgcc tcgccccttg gagtaatctc ccgccatgac cggaaccttg
     4141 gggaccgtcg tctcattcct gattggcctg agcgcgctgg ccacgccggt tggaatcgcg
     4201 ctagccgccg agtcggattc cgtcacggtg accgcggggg cgcactacgg gcgctccggc
     4261 ttccaccgat tcttcttcgg ctccgactat cggaaggtct ggaccacgcc gattcgcatc
     4321 cccgtgctgg acatgagccg ggaagcggga ggactccgcc ccgtgcggcg cgtcgggggc
     4381 cggcagaccc aggcgctcgc gctcgcgggc gcggacggcc ggagctacac cttccgcggc
     4441 gtggcgaagg atgtctccgg actcatcgac gaaggacttc gcggcacgat cgtcgagagc
     4501 atcctcaagg atcagatggc ggcgcagcat cccgccggcg agctgatcgc cgggagcctg
     4561 gcgaaggcgg cggggatccg gacgcaggaa tgggtgctgg tcgcgctccc ggacgatccc
     4621 gcgctcgggg aattccgcga tgagttcaag ggggcgatcg gattcttcgg ggaatatccc
     4681 accgcgccga ccggcgagcg cccgggaacc ttcggtctca aggagatcgt cgggtacaag
     4741 gagatctatc tcaagctcga gaccgatccc tcgacgcaca tcgacgcggg cgaatttctg
     4801 tacgcgcggc tgttcgatct cgcgatcggc gactgggacc ggcaccggcg ccagtggcgc
     4861 tgggcgcgat tcgaaggcga tccgctctgg catcccattc ccgaggaccg ggatcaggcg
     4921 ttctcgcgct acgaggggct tttcctgggc atcggccgcc gtacgcgcga ccaacgcttt
     4981 cagaagtacg acgatcacta caagagcgtg tccggtctca tgtggaa
//
LOCUS       DASTKC010000120        19187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1270332, whole genome shotgun sequence.
ACCESSION   DASTKC010000120 DASTKC010000000
VERSION     DASTKC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 19187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19187
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270332"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="VFR25_04995"
     CDS             <1..1399
                     /locus_tag="VFR25_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9940437.1"
                     /translation="AKSADKPKSDAKATPAPATAPRVDTPDTRKYPTNRAAELKARLL
                     ERPKDPAAAAWAVEYAGTPEVARLDPVTRAAEQAWAARTGLASGPATAERPALEAILT
                     QVGSCPLDSVPVKRAEEGLTRIGVVVPLSGKYERFGRTFVNGLRVAMEEHNRDWAPRL
                     SLILHDSEGDPLVGARKARWLLKDHGVSLLVGEILSANTAPLAAATQVTDAVLISPSA
                     TNERLAILGDGVFQLHIGTKPLAESLARQLATESPNSSVGILVAKTHDDSLLAAAVSR
                     SCDNAAVRVMGTERVREGDADLTKELTKLRDKKPTALVLIGSQQFVSLSGAQLRSIWP
                     DAHVLGFQSMDPEPLNEESRHGLEGAILFLSEYALDGDPKTAFESAYRRVNKEPPTRM
                     SVRGYLTGLAIARAIESGAFTASQLKEALRTQVYDDDEERALRSLKPAVAAFPERLTI
                     KGGKAVPLAQAPVDP"
     gene            1489..2682
                     /locus_tag="VFR25_05000"
     CDS             1489..2682
                     /locus_tag="VFR25_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9940438.1"
                     /translation="MIRSLRPWWLALALVLLLGLPTGCAKKSNEIVIGEYGSLTGTTA
                     TFGISSKNGIELYLDNLNAAGGVGGTKVKVIVEDDQSKPEEAATAVNKLVDQDGVIAV
                     LGEVASSRSMAAAPICQQSGVPMISPSSTNPKVTEIGDYIFRVCFIDPFQGQMIAKFA
                     KEKLGLTKAAVFRDIKNDYSVGLANYFTDAYHALGGTIVADEAYSEGDQDFKAQLTVI
                     KSKKPQVLIVPGYYTEVGLIARQAREMGLDVPMVGGDGWVSDKLIEIGQDALNGCYHV
                     NHYWEEDPNPAIQKFVTEYKSRYNAKPDGLAALAYDAAGVLTSALDRLRTEDPAAFKA
                     LLGPRNEAQKAARAKLRDRIASTKDYPGVTGRITLDAARNAVKPAVFVGIKNGKYEFV
                     AAVEP"
     gene            2700..3605
                     /locus_tag="VFR25_05005"
     CDS             2700..3605
                     /locus_tag="VFR25_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9940439.1"
                     /translation="MQELFQQLANGIAWGSIYALIALGYTMVYGILRLINFAHGDVYM
                     VGAFAAYYIASATGAGGAGASLPMALLVLLGAMLACALLGMLIELLAYRPVRRSSRIT
                     ALITAIGVSLFLENFGLRVFGADPKFFPQLIPPKRIMLAGGVMVTNHQITVVVVSILL
                     MVALTLFIQRTKTGKAMRAVAFHRDAASLMGIPVNRIITITFAIGSALAAAAGVLVGL
                     TNPKIEPLMGIMPGIKAFVAAVLGGIGSVPGAVIGGLVMGVSEYLVVGYISSTYRDAI
                     AFVILILVLLIRPAGLLGRNVAEKV"
     gene            3586..4551
                     /locus_tag="VFR25_05010"
     CDS             3586..4551
                     /locus_tag="VFR25_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9940440.1"
                     /translation="MSPRKSRFARGLLWVAAALALSLGVDRLAVNWLNPYFYRILVLC
                     GLNVMLALSLNLVNGITGQFSIGHAGFMAVGAYASAAFTVYAVPQLFGVDPNASGLVV
                     VAPLFLALLVGGLTSAAAGFLVGLPSMRLRGDYLAIVTLGFGEIIRVAILNIDAVGGA
                     RGFAGIPRRTDLAWVIAGALATWVVLRNLLRSYHGRAFLAIREDEIAAEALGVPTTRY
                     KVTAFVVAAFFAGVAGALFAHYTYLHTNSFTFMKSIEVIVMVVLGGMGSLLGSVLGAT
                     VLTALPEALRFASSERLIIYSLLLIVLMIARPQGILGKTEGKRGK"
     gene            4548..5318
                     /locus_tag="VFR25_05015"
     CDS             4548..5318
                     /locus_tag="VFR25_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940441.1"
                     /translation="MSGPLLELRRVTRAFGGLRAVADLDLTLDSGLLAGLIGPNGAGK
                     TTVFNLITGVYAPTSGEIRFEAKRIDGRATSSIARAGIARTFQNPRIFAGLTCLDNVR
                     IACHTHAGAGLLDSVLATGRSAAEEADILARSEELLRSFALDGYRDTPAGGLPYGEQR
                     RLEIARALAAEPRLLLLDEPAAGLNPHETQALMRLIHDVRDRYKLTVLLIEHDMKVVM
                     GICERITVLDYGVCIAEGEPAAIRSDPKVIEAYLGDAV"
     gene            5315..6031
                     /locus_tag="VFR25_05020"
     CDS             5315..6031
                     /locus_tag="VFR25_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940442.1"
                     /translation="MSLLDLENVHVHYGAIHALKGVTIAVEPGQIVTLVGANGAGKST
                     TLRAISGLLRPSQGTIRFHGKSLAGCPAHDIVKLGISHVPEGRIVFANLSVEDNLELG
                     AYLRKDRAGVASDRKKIYALFPRLEERRRQSSGTLSGGEQQMLAIGRALMARPKLLLM
                     DEPSLGLAPILVREIFRVIREIREQGMTIVLVEQNARMALSVADRGYVLETGVVCLAD
                     RADALLQNPEVQAAYLGGAV"
     gene            6028..7425
                     /gene="selA"
                     /locus_tag="VFR25_05025"
     CDS             6028..7425
                     /gene="selA"
                     /locus_tag="VFR25_05025"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET9940443.1"
                     /translation="MSRLRSIPSVESLLQHPDLAGLAERAPRPVIVQAVRDTLARQRE
                     SLRGGAAAMSPDELASRARALAEASLRPNLRPVINATGIILHTNLGRAPLPEAAVEAV
                     QMAARGYSNLELDLATGSRGSRGAHIEPLLRELLGAPAAIAVNNNAAAMLLALNTLAA
                     GREAVVSRGELVEIGGSFRIPEILERAGARLREVGTTNKTRLSDYERAIGPNTGVVLR
                     VHPSNFAVVGFAERVERDDLVGLARKKKVPLLEDVGSGAMVDLRPFGLPEEPLLRESL
                     RDGVPLACASGDKLLGGPQAGILAGTKKLVDACRSNPMARALRLDKLSIAALEATLRL
                     YRDPEAIGSAVPVLRMIAEPASAVRARARRLIASLGAERASRARAEVVPCTAEVGGGS
                     MPLARIPSFAVALSATGGTIADLARTLRLGAEPVLSRIESGRLLLDLRTVDHRELKRL
                     VAALHTALDGKRGIG"
     gene            7422..9131
                     /locus_tag="VFR25_05030"
     CDS             7422..9131
                     /locus_tag="VFR25_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET9940444.1"
                     /translation="MIEAEVERYSDPHSFAREVNLPLLGTARIEPVPGSPPMSRLLEV
                     AAPPELEAVLEGLTETRRSLTLRSLLLAGFPEDRECFAIGLALARAWSRRGLKIAVVD
                     LDFWNPTVVRPRPNPNEGLVDVLEYGCSFRRVAWEIVAQGLWVVGPGSYPPDPHRVVE
                     HPDWERAARSFASHVDVTLYVAPLLERSGFVGRLSKRMDGVLLAASVERIARTDLRDA
                     FLELWGSDAPMIGIIGIEGALTGSREANEPAGTDVGVASVTVAEPEWIGAPEWIGQPA
                     PVDVPYAEPETRGGSYGDDRREPVPSAVAWSSSRDDSVERELASILQREVELGRPLVK
                     PIRRSRAGLYAGVAAGLAVAASIVAVLMRHPTLPPGPEVIPAGNEEVLPAPMEGQSAA
                     WGSASSLPAETSPEATTAPSPSQGATAPQAAAIAGGSSVPARATSKPPQASPAPTHAS
                     SPPPQTSSAPAKASSKPPQAKSIPPQATSTAPVAAAASGAKPFRVHVASFKSEAKVEE
                     IVRGLKAKGADAYYEKAADAPGYYRVFVGHFATEKEAAAQAQKLLEQGWVDRAQAFPL
                     TAR"
     gene            9135..12893
                     /gene="smc"
                     /locus_tag="VFR25_05035"
     CDS             9135..12893
                     /gene="smc"
                     /locus_tag="VFR25_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET9940445.1"
                     /translation="MFLQRLDIQGFKSFPQRVRVDFGPGITAVVGPNGSGKTNIVEAI
                     RWVLGEQNMRHLRGDTLEDVIFTGSAHRKPLGMAEVSLTMVNNRGVLPSEYTEVAIAR
                     RTFRSGQSEYLINRNPCRLRDVRDLFLDTGMGSHAYSLIERGMVDNVLSDESGHRRFL
                     FEEAAGIMRYKTRKKEALQKLDLTHADLTRVNDITVEIEREVRSLARQVGKARRYGKL
                     RDEIRGVDLGLAREAFDRLSREAGTLHQERHNAENRRSSLGGTLAHHEAELEELKLEL
                     LKREGEVRLAQEALSEHEARGAALLNDVAVLRERRLGLVEKLEHARSEADRLEKSVEE
                     VRGHATRLDLDRARLLDEQALRETEERRLDEELSAMGPKLDERRAALAERKQLSLDLF
                     EARVMQESSARNWREKLEDLGKRRGEIEAQRDALAKDEDLLRAGIRDIERALEETQGD
                     VERGRERVERAVEAIARAEARIREHDDVEIKAREAHAGLEARHATLRELKERYEGYDA
                     SVRWLVAGPSRPPRVLGTVGDVLHASGDWLPALEAALGEAVQFVVAERTEAAVHALRA
                     LEQSGEGRATFISLDRLSRVHAAQISAEVLSAPGVLGPLLDHVRFEPGYVALASFLLS
                     SVVVVDTIDAGLALNERFQGERLQFVTRRGERVVGPGIFQGGGGPTRAGSVLRREEEL
                     TDLSAEIERSAASLAAILKTTEGNRAERASAVSEQEEAARLLQVAEQAHRAQESRLAE
                     QKIRGDAMVQASSSLASSLGAVAAELERALGEAESAQVALKSADEERGRVDAILAQEE
                     TEVRELERNREHRVALHAEARVEATRARSSLEAAIRERERLTRSADEAEREIGSRREE
                     AIQTETRIQETETLCGERETELAQEIEKKAEREVLLQRARERFGEVKGQADDVESTTR
                     DVRREHATLTERLHKAELEQAEADGELRRLLERVRNEYEIDLVSWAPEAEAVPGDSAD
                     PGDPALAEIEEGDVELEPDSPRVPDEAIVDRDDAASGSAPITAAATREEREARLRYLQ
                     EKLRSIGPVNLLAVQDYEERRQRLGFLNGQRKDLDDARQQLLEAIEKINATASELFLK
                     TFEQVNKNFVQVFTTLFQGGEAALVLTGDDPLEAEIEILARPRGKKPQSLSLLSGGEK
                     ALTAIALLFAIYLEKPSPFCILDEVDAPLDDANIDRFVGLLRDFSAKTQFIVVTHNKK
                     TMEVADALYGVTMQEPGVSKLVSVRWNRERQDVDQVDAPPEELAPAAS"
     gene            12905..13816
                     /gene="ftsY"
                     /locus_tag="VFR25_05040"
     CDS             12905..13816
                     /gene="ftsY"
                     /locus_tag="VFR25_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9940446.1"
                     /translation="MQFFKKITEGLKKTRDAFVERVSDVLKPGKKLEPEDYEALEAAL
                     LRADVGPATSDRILDDVKERLAKGADDPALALREAVRAVVEPPAPSAANVPRPHVTLV
                     VGVNGTGKTTTIAKLAHRYKEAGESVLLVACDTFRAAAGDQLEVWAERAGVEMVRQAS
                     GADPAAVAFDGMARAKSRNVQRAIVDTAGRLHVKANLMAELDKIRRVIERGIPGAPHE
                     TLLVLDATVGQNGLQQAKEFGRILPITAIALTKLDGTAKGGVVLAIADALKIPIRWVG
                     VGEGMDDLVPFDPEEYAAALTGSYSPA"
     gene            complement(13801..15252)
                     /locus_tag="VFR25_05045"
     CDS             complement(13801..15252)
                     /locus_tag="VFR25_05045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9940447.1"
                     /translation="MNDPQRIRTALLASLCLSEASTEDRRHLIELSLTDLDGEARDRL
                     AGEIAERLLEGGSLERVDRFQRDGRPYVRYRVPATNETFLLPAPLELREETLVRAVRP
                     HWHSRSRLLYKSLSHLIAVERKLFTRDERNTEYLQTDIGDILRTILTSARDVLECDDV
                     RLFPLGSTPDEARYAQPLSDGEPWDKALAVEWVGKRMLPVYVADLAEAGRIETMLDTE
                     AVDATVHVAIAPYEPRPVPGGFRSVAMARVGEPGTPFLYVLEAWSKAPRFFSEERLGI
                     LGIVTEHATDLLNTLKKLGMLVMVDELTGIYNRPYFARQLDHEIARAYREERPMSLVI
                     ADIDDFKRVNDTLGYEAGNAILRELGRTLAGSVRPFDTVARWGGEEFALILSAETTKL
                     EAKEICERLRLKVENLAVAVPGLDGRETTVRVSMSLGGAMFPADVPVTWDRSRGLDRG
                     QREKLAHDLWTRANLHLRLSKERGKNQVTYAGE"
     gene            15594..15917
                     /locus_tag="VFR25_05050"
     CDS             15594..15917
                     /locus_tag="VFR25_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940448.1"
                     /translation="MKRSRGKPHPWPTWVGAALGILVLTSAPPRIQARPYNPEYPPMP
                     DGDPTADDQPSPTPKGGGGHYATYRNDADLTARKPGATMTASQLRGRLIWLSFVRTWI
                     WIGMR"
     gene            15970..18303
                     /locus_tag="VFR25_05055"
     CDS             15970..18303
                     /locus_tag="VFR25_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9940449.1"
                     /translation="MSYDKVPRSDVASVLQKIDDLLSRARFTAALEVIAGFRAGRSVP
                     EALDTPLLLARCRALLGLGRWKEVADAAEKKLSELYALRPEEKRSLLEFHIAAGRAVW
                     RLGRPSRAEEHFRAAYHISRWEFDDAAGMLRSRNLLGLCFLGAGEIHRAVGEFARGQT
                     QAREAGLVHEEASFSLNLSIALGKNGRLLEADKELARARTLFAERGHSRGKVQARLFH
                     GYLARIRGDAREAELQAQGALSEAEEHGFERERVIALEYLGDLALDRHDNQGAVDNLE
                     QAFILAERIAPEGDLIPELCRRTAEVHVRIGEPNRALVACERGLRVARAIHDRFEEAA
                     AQRVMAMAHLLLGHREKAARIAQEGAMLLRKLEANYELMRLLVWTGEMLVAGGDSEER
                     REGRDALWEARSLSMAMKLDRWLERIERDLGVEIVPTAGPSPADHALDCAHPSGADPD
                     SCRWGIITQDPRMIELLAILERASSSRLPILVLGESGTGKELLARAAHGLSDRATRPF
                     VVGHCAALSDGLLDVELFGKDPGEGPGGEGARTGLFETASRGVMFLDEVGELLTSAQA
                     KLLRVIEMGELRRVGGMGVRQVDVRVIVASTRDLGSLVRRGLFRDDLYYRLNGIRLEV
                     PPLRERPGDIGLIGRHLLDRACLEAGKRVTLSEEAWARLIAHPWPGNVSELKSVIERT
                     VALAGDGERLSAEQVQFQPAVRAPISAMTERTGIRTMRRSGDPEERNRIVDALRANGG
                     NQSEAARSLNGMKRTTLLYKMKKLDIRPEEYRPAPPR"
     gene            18425..18880
                     /gene="nuoI"
                     /locus_tag="VFR25_05060"
     CDS             18425..18880
                     /gene="nuoI"
                     /locus_tag="VFR25_05060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET9940450.1"
                     /translation="MVSELMKGLSITLSHFFKKPVTLKYPDVKMPMYPRFRGLHELHR
                     YANGLERCVCCGLCAAVCPADAIYMEAAENTPTQRFSAGERYARRYEIDMLRCIFCGY
                     CEEACPEDAIFLGHNFELSDYSRDSFLYSKEQLLVPVGMTNTPGIVRGR"
     gene            18896..>19187
                     /locus_tag="VFR25_05065"
     CDS             18896..>19187
                     /locus_tag="VFR25_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940451.1"
                     /translation="MGDRWLLAAACGVVAGLANVLGGWLAVGRHGWDRVRQSYFMAAG
                     AGFLLAAVFLRLIPESFRLASNPNGPPTLAVGSLILVGYLLIHAAEHVLVGHF"
BASE COUNT         2953 a         6677 c         6659 g         2898 t
ORIGIN      
        1 cgcgaagtcc gcggacaagc cgaagagcga tgcaaaggcc acccccgcgc cggcgaccgc
       61 gccacgggtc gacacgcccg acacccggaa gtatccgacg aatcgggcgg cggagctcaa
      121 ggcgcggctc ctcgagcgcc ccaaggatcc cgcagcggcc gcatgggcgg tcgagtacgc
      181 cggcacgccc gaggtggcgc gcctggatcc cgtgacccgc gccgccgagc aggcatgggc
      241 cgcgcgcacg gggctcgcgt ccggccccgc gaccgcggag cgtccggcgc tcgaggcgat
      301 cctgacccag gtcggctcgt gcccgctcga ctcggtgccc gtgaagcgcg cggaggaagg
      361 gctcacccgc atcggcgtgg tggtgcctct gagcgggaaa tacgagcgct tcggccgcac
      421 ctttgtgaac ggactcaggg tcgcgatgga agaacacaac cgggactggg cgcccaggct
      481 cagcctcatc ctccacgact ccgagggcga tccgctcgtg ggagcgcgca aggcgcgctg
      541 gctcctcaag gaccacggcg tctcgctcct cgtgggggag atcctgagcg cgaacacagc
      601 gcccctcgcg gccgccacgc aggtgacgga cgcggtgctc atctcgccct cggccaccaa
      661 cgagcggctc gcgatcctcg gcgacggcgt cttccagctc cacatcggca cgaagccgct
      721 cgccgaatcg ctcgcacgcc agctcgcgac ggagtcgccg aactcctcgg tcgggatcct
      781 cgtcgcgaag acgcacgacg actcgctcct cgccgcggcc gtctcgcggt cgtgcgacaa
      841 cgccgccgtg cgggtcatgg gaaccgagcg ggtgcgggag ggcgacgcgg atctcacgaa
      901 ggagctgacc aagctcaggg acaagaagcc gacggctctc gtgctgatcg gctcgcagca
      961 gttcgtcagc ctctcgggag cgcagctccg gtcgatctgg ccggacgcgc acgtgctcgg
     1021 cttccaatcg atggatcccg agcccctgaa cgaggagtcc cgccatgggc tcgaaggggc
     1081 gatcctcttc ctgtcggagt acgcgctgga cggcgacccg aagaccgcgt tcgagtcggc
     1141 gtacaggcgc gtcaacaagg agccgccgac ccggatgtcg gtgcgtgggt atctcacggg
     1201 acttgcgatc gcgcgggcga tcgagtcggg cgcgttcacc gcgtcgcagc tcaaggaggc
     1261 gctccgcacg caggtgtacg acgacgacga ggagcgcgcg ctgcgctcgc tcaagccggc
     1321 ggtggccgcg ttccccgagc gtctcacgat caagggcggc aaggcggtgc ctcttgccca
     1381 agcgccggtt gacccttgaa acgcccggtc ccatcggcta gggtgccggt ggtatcgggc
     1441 acgacaatca cattgaaagc caaacactta cgtcatggag gatggagcat gatccggtct
     1501 cttcgacctt ggtggctcgc cctcgcgctc gtcctcctgc tcggcctccc gaccggctgc
     1561 gccaagaaga gcaacgaaat cgtgatcggc gagtacggat cgctcacggg gaccacggcc
     1621 acgttcggaa tctcctcgaa gaacgggatc gagctctacc tcgacaatct gaacgcggcg
     1681 ggcggggtcg gtggcaccaa ggtgaaggtg atcgtcgagg acgatcagtc gaagcccgag
     1741 gaagccgcga cggccgtgaa caagctcgtc gaccaggacg gcgtgatcgc ggtgctgggc
     1801 gaggtggctt cgagccgcag catggcggcc gctccgatct gccagcagtc gggcgtgccg
     1861 atgatctctc cctcctcgac caatcccaag gtcaccgaga tcggcgacta catcttccgc
     1921 gtctgcttca tcgatccctt ccagggacag atgatcgcga agttcgccaa ggagaagctc
     1981 ggcctcacga aggcggcggt cttccgcgac atcaagaacg actactcggt cggtctcgcg
     2041 aactacttca cggacgccta ccacgcgctc ggcggaacga tcgtcgcgga cgaggcctac
     2101 tccgaggggg atcaggactt caaagcgcag ctcaccgtca tcaagtcgaa gaagccgcag
     2161 gtcctcatcg tgcccggcta ctacaccgag gtgggcttga tcgcgcgcca ggcgcgcgag
     2221 atggggctcg acgtcccgat ggtcggcggg gacggttggg tctcggacaa gctgatcgag
     2281 atcgggcagg acgcgctgaa cggctgctac cacgtgaacc actactggga agaggatccg
     2341 aacccggcca tccagaagtt cgtgaccgag tacaagagcc gctacaacgc gaagccggac
     2401 ggtctcgccg cactcgccta tgacgcggcc ggcgtgctga cctcggcgct cgaccggctc
     2461 cgcaccgaag atcccgccgc cttcaaggcg ctccttggtc cgcggaacga ggcgcagaag
     2521 gcggcgcgcg cgaagctgcg tgaccggatc gcctcgacga aggactatcc gggcgtcacc
     2581 gggcgcatca cgctcgacgc ggcgcggaac gccgtgaagc ccgccgtctt cgtcgggatc
     2641 aagaacggca agtacgagtt cgtggcggcg gtcgagccct aaggtccgcc ccagagcgcg
     2701 tgcaggagct cttccagcag ctcgccaacg gcatcgcgtg ggggagcatc tatgccctca
     2761 tcgcgctcgg ctacaccatg gtgtacggca tcctgcgcct catcaacttc gcgcacggtg
     2821 acgtctacat ggtgggcgcg ttcgccgcct actacatcgc gagcgcgacc ggcgccggcg
     2881 gcgccggggc ttccttaccg atggcgctcc tcgtcctcct cggagcgatg ctggcgtgcg
     2941 cgctcctcgg gatgctgatc gaactcctcg cctaccgacc cgtccggcgc tcctcgagga
     3001 tcacggcgct catcaccgcg atcggcgtct ccctcttcct cgagaatttc gggctccgtg
     3061 tcttcggcgc cgatcccaag ttcttcccgc agcttatccc gccgaagcgg atcatgctcg
     3121 ccggcggtgt gatggtcacg aaccaccaga tcacggtcgt ggtcgtctcg atcctcctca
     3181 tggtggcgct gacgctcttc atccagcgaa ccaagacggg gaaggcgatg cgcgcggtcg
     3241 cgttccacag ggatgccgcg agcctcatgg gcatcccggt gaaccggatc atcacgatca
     3301 cgttcgcgat cggctccgcg ctcgccgccg cggcgggcgt cctcgtcggg ctcacgaatc
     3361 ccaagatcga gcctctgatg ggaatcatgc cgggcatcaa ggcgttcgtg gccgcggtgc
     3421 tcggcggcat cggcagcgtt cccggagcgg tgatcggcgg cctggtgatg ggggtctcgg
     3481 aatacctcgt tgtcggttac atctcctcga cctaccgcga cgcgatcgcc ttcgtcatcc
     3541 tgatcctcgt cctcctgatc cggccggcgg ggctcctggg aaggaatgtc gccgagaaag
     3601 tctaggttcg cgcggggcct cctctgggtg gccgccgcgc tcgccctctc cctgggggtc
     3661 gaccggctcg ccgtcaactg gttgaatccc tacttctacc ggatcctcgt cctctgcggc
     3721 ctcaacgtga tgctcgcgct gagcctcaat ctcgtgaacg gaatcacggg gcagttctcg
     3781 atcgggcacg cgggcttcat ggccgtcggc gcctacgcgt ccgcggcctt caccgtctac
     3841 gccgtgccgc agctcttcgg cgtcgacccg aacgcaagcg gcctcgtcgt cgtcgcgccg
     3901 ctcttcctgg cgctcctcgt cggcggactg acgagcgcgg ccgccggctt cctggtcgga
     3961 ctcccgtcga tgcggcttcg cggcgactac ctcgccatcg tcaccctcgg cttcggcgag
     4021 atcatccggg tcgcgatcct gaacatcgat gccgtcggcg gggcgcgcgg cttcgcgggc
     4081 atcccgcgcc gcaccgacct cgcctgggtg atcgccggcg cgctcgcgac gtgggtggtg
     4141 ctccggaacc tcctccgctc gtatcacggc cgcgcgttcc tcgcgatccg cgaggacgag
     4201 atcgctgccg aagcgctggg tgttccgacc acacgctaca aggtgaccgc cttcgtggtc
     4261 gcggccttct tcgccggcgt cgccggcgcg ctcttcgcgc actacacgta tctccacacg
     4321 aacagcttca ccttcatgaa gtcgatcgag gtcatcgtga tggtcgtgct cgggggcatg
     4381 gggagcctcc tgggatcggt cctcggcgcg acggtcttga ccgcgctccc ggaggcgctc
     4441 cgcttcgcga gctcggagcg gctcatcatc tactcgctcc tcctgatcgt gctcatgatc
     4501 gcgcggccgc aggggattct cgggaagacc gaggggaagc gcgggaagtg agcgggcctc
     4561 tcctcgagct caggcgcgtc acgcgcgcgt tcggggggct ccgcgcggtg gccgacctcg
     4621 atctcaccct cgactccggt ctcctcgccg gcttgatcgg tcccaacggg gcggggaaga
     4681 cgaccgtgtt caatctcatc accggcgtct acgcgcccac gtcgggcgag atccggttcg
     4741 aggcgaagcg gatcgacggc cgggcgacgt cctcgatcgc gcgcgcgggc atcgcccgca
     4801 cgttccagaa tccgcgcatc ttcgcgggac tgacctgcct cgacaacgtg cggatcgcgt
     4861 gccacacgca cgcgggcgcg ggcctcctcg acagcgtcct cgcgaccggc cggtcggcgg
     4921 ccgaggaggc ggacattctc gcgcgctccg aggagcttct tcgatccttc gcgctggacg
     4981 gctatcgcga cacgccggcc gggggacttc cgtacggcga gcagcggcgg ctcgagatcg
     5041 cgcgggcgct cgcggcggag ccgaggctcc tcctcctcga cgagccggcg gccggcctca
     5101 atccccacga gacccaggcc ctgatgcggc tcatccacga cgtgcgggac cgctacaagc
     5161 tcacggtcct cctgatcgag cacgacatga aggtggtcat ggggatctgc gagcggatca
     5221 cggtgctcga ctacggggta tgtatcgccg agggggagcc ggcggccatc cggagcgacc
     5281 ccaaggtgat cgaggcgtac ctgggagacg cggtgtgagc cttctcgacc tcgagaacgt
     5341 ccacgtccac tacggcgcga tccacgccct gaagggtgtg acgatcgcgg tcgagccggg
     5401 gcagatcgtg accctggtcg gtgcgaacgg ggcggggaag agcaccaccc ttcgcgcgat
     5461 ctcggggctc ctccgtccga gccaggggac gattcgcttc cacgggaagt ccctggcggg
     5521 ctgccccgcg cacgacatcg tgaagctcgg catctcccac gtgcctgaag ggcgcatcgt
     5581 gttcgccaat ctgagcgtcg aggacaacct cgagctgggc gcctatctga ggaaggatcg
     5641 tgcgggcgta gcctctgacc ggaagaagat ctatgcgctc tttccgaggc tcgaggagcg
     5701 ccggcgccag tcgtcgggca ctctgagcgg cggcgagcag cagatgctcg cgatcggccg
     5761 cgccctgatg gcgcgcccga agctcctcct catggacgag ccctcgctcg gcctggcgcc
     5821 gatcctcgtc cgcgagatct tccgcgtcat ccgcgagatt cgcgagcagg ggatgacgat
     5881 cgtgctcgtg gagcagaacg cgcggatggc gctctcggtg gcggatcgag gctacgtgct
     5941 cgagacgggc gtcgtgtgcc tggccgatcg cgcggacgcg ctcctccaga atcccgaggt
     6001 gcaggcggcc tatctcggcg gcgcggtgtg agccggctcc gctcgatccc gtcggtggag
     6061 tcgctcctcc agcaccccga cctggccggc ctcgcggagc gcgctccgcg ccccgtcatc
     6121 gtgcaggcgg tgcgcgacac gctcgcgcgc cagcgggaat cgctgcgcgg cggggcggcc
     6181 gcgatgtctc cagacgagct ggcgtcgcgg gcgcgcgcgc tggccgaagc ctcgcttcgc
     6241 ccgaatctcc gccccgtcat caacgcgacc gggatcatcc tccacacgaa tctcggccgc
     6301 gccccgctcc cggaagcggc ggtcgaggcg gtccagatgg cggcgcgcgg gtactcgaat
     6361 ctcgagctcg acctcgccac gggctcgcgc ggctcgcgcg gcgcgcacat cgagcccctc
     6421 ctgcgcgagc tcctcggcgc tcccgccgcg atcgcggtca acaacaacgc ggcggcgatg
     6481 ctcctcgccc tgaacacgct cgcggccggg cgcgaggcgg tcgtctcgcg cggtgagctg
     6541 gtcgagatcg ggggctcgtt ccgcattccg gagatcctgg agcgggcggg cgccaggctg
     6601 cgcgaggtcg ggaccaccaa caagacgcgc ttgagcgatt acgagcgcgc gatcggcccg
     6661 aacaccggcg tcgtgctccg cgtccatccg agcaacttcg cggtggtggg cttcgcggag
     6721 cgcgtggagc gggacgatct ggtggggctc gcgcggaaga agaaggtgcc gctcctcgag
     6781 gacgtgggca gcggcgccat ggtcgacctt cgcccattcg gcctcccgga agagccccta
     6841 ctccgagagt ccctgcgcga tggcgttccg ctcgcgtgcg cgagtgggga caagctcctc
     6901 ggcggaccgc aggcggggat cctcgccggg accaagaagc tcgtcgacgc gtgccggtcg
     6961 aatccgatgg cgcgcgcgct ccggctcgac aagctctcga tcgcggccct cgaagcgacg
     7021 ctccgcctct accgcgatcc ggaggcgatc gggagcgcgg tgccggtgct ccgcatgatc
     7081 gcggagcccg cctccgcggt gcgcgcccga gcgcgccggc tgatcgcgtc gctcggagcg
     7141 gaacgcgctt ctcgcgcccg ggcggaggtg gtaccttgca ccgcggaggt cggcggcggc
     7201 tcgatgccgc tcgcgcggat cccgtcgttc gcggtcgcct tgagcgcgac cggcgggacc
     7261 atcgcggacc tggcccggac actccgcctc ggagccgagc cggtcctatc gcgcatcgaa
     7321 tcgggccggc tcttgcttga cctcaggacg gtcgatcacc gcgagctcaa gcggttggtc
     7381 gccgcgctcc acaccgcgct ggacgggaag aggggaatcg ggtgatcgaa gcggaggtcg
     7441 agcgctactc cgatccacat agcttcgccc gggaggtcaa tctcccgctc ctcggcacgg
     7501 cgcggatcga gcccgtaccg ggcagccccc cgatgtcgcg cctcctcgag gtcgccgcgc
     7561 cccccgagct cgaggcggtg ctcgaagggc tcaccgagac ccgccgctcg ctcacgcttc
     7621 gctcgctcct cctcgcggga ttccccgagg atcgcgagtg cttcgccatc ggactcgcgc
     7681 tcgcgcgcgc ctggtcccgc cgcggactca agatcgccgt ggtcgacctc gatttctgga
     7741 atcccaccgt cgtgcgtccg cgcccgaatc ccaacgaggg gctggtggac gtgctggagt
     7801 acggctgctc gttccgccgc gtggcctggg agatcgtcgc gcaggggctc tgggtcgtcg
     7861 ggcccggcag ctacccgccc gacccgcacc gcgtcgtcga gcatccggac tgggagcgcg
     7921 ccgcgcggag cttcgcctcg cacgtggacg tgacgctcta cgtggcgccg ctcctcgagc
     7981 gcagcggctt cgtgggccgg ctctccaagc ggatggacgg agtgctcctc gccgcgtcgg
     8041 tcgagcggat cgcgcgcacc gatctaaggg acgcgttcct cgagctctgg ggatccgacg
     8101 ctccgatgat cgggatcatc ggcatcgaag gcgccctcac gggatcgcgc gaggcgaacg
     8161 agcccgccgg gacggatgta ggagtcgcgt cggtgaccgt cgcggagcca gagtggatcg
     8221 gggcccccga atggatcggc cagccggctc cggtcgacgt gccctacgcg gagccggaga
     8281 ctcgaggcgg ctcgtacggg gacgatcgcc gcgagcccgt gccttccgct gtcgcgtggt
     8341 cctcctcgag ggacgattcg gtggagcgcg agctcgcatc gatcttgcag cgcgaggtcg
     8401 agctcggacg tccgctcgtg aagcccatcc ggcgctcgcg cgcaggcctc tacgccggcg
     8461 tggccgcggg actcgccgtc gcggcgagca tcgtcgccgt gctgatgcgc catcccacgc
     8521 tccctccggg ccccgaggtg attccggcgg ggaacgagga ggtcctgccc gcgccgatgg
     8581 aggggcagtc cgcggcctgg ggtagcgctt cgtcgcttcc tgccgagacc agcccggagg
     8641 ccacgacggc gccctccccg tcccagggag cgacggcgcc ccaggcggca gccattgccg
     8701 gaggaagctc cgtgccggcg agggcgactt cgaagccgcc acaagcgagc cccgcaccga
     8761 ctcacgcaag ctcgccgccg ccacagacga gctcggcgcc ggcgaaggca agctcgaagc
     8821 cgccacaggc gaagtcgatc ccgccgcagg cgacctcgac cgcgcccgtt gccgccgcag
     8881 cctcaggcgc caagccgttc cgcgttcatg tcgcgtcgtt caagtccgag gccaaggtcg
     8941 aggagatcgt gcgcggcctc aaggccaagg gggcggacgc ctattacgag aaggccgcgg
     9001 acgctcccgg ctactaccga gtcttcgtgg gacatttcgc caccgagaag gaggccgccg
     9061 cccaagccca aaagctcctc gagcagggct gggtcgatcg cgcccaggcc tttcctttga
     9121 ccgcgaggta aaccgtgttt ctccagcgcc tcgacatcca gggcttcaaa tccttcccgc
     9181 agcgagtccg cgtggacttt gggccaggca tcacggcggt cgtgggtccc aacggcagcg
     9241 gcaagaccaa catcgtggag gcgatccgct gggtgctggg cgagcagaac atgcgccacc
     9301 tgcgaggcga cacgctcgag gacgtgatct tcaccggctc cgcgcatcgg aagccgctcg
     9361 gcatggccga ggtctcgctc acgatggtca acaaccgcgg cgtgctcccc tcggagtaca
     9421 ccgaggtggc gatcgcgcgg cgcacgttcc ggagcgggca gagcgagtac ctcatcaacc
     9481 ggaacccctg ccggcttcgc gacgtgcgcg atctcttcct cgacacgggc atgggatcgc
     9541 acgcgtattc gctcatcgag cgcgggatgg tcgacaacgt cctctcggac gagagcggcc
     9601 atcgccggtt cctcttcgaa gaggcggccg gcatcatgcg ctacaagacg cggaagaagg
     9661 aggcgctcca gaagctcgac ctgacgcacg ccgatctcac gcgcgtgaac gacatcacgg
     9721 tcgagatcga gcgcgaggtg cgctctctcg cgcggcaggt ggggaaggcg cgccgctacg
     9781 ggaagctccg cgatgagatt cgcggcgtgg atctcggtct cgcgcgcgag gcattcgatc
     9841 gcctctcccg cgaggcgggg acgctccatc aagagagaca caacgccgag aaccggcgct
     9901 cttcgctcgg cggcacgctg gcccaccacg aagccgagct cgaggagctg aagctcgagc
     9961 tcttgaagcg ggagggcgag gtcaggctgg cgcaggaggc gctgagcgag cacgaggcgc
    10021 gcggcgcggc gctcctgaac gatgttgccg tgctccgcga gcggcgcctc ggactcgtcg
    10081 agaagctcga gcacgcgaga tccgaggcgg atcgcctcga gaagagcgtc gaggaagtgc
    10141 gcggccatgc gacgcggctc gatctggacc gggcgcggct cttagacgag caagcgttgc
    10201 gcgagacgga ggagcggcgg ctggatgagg agctctcggc catggggccg aagctcgacg
    10261 agcggcgcgc cgccctcgcc gagcggaagc agctctcgct cgacctcttc gaggcgcgcg
    10321 tcatgcagga gagctcggcg cgcaactggc gcgagaagct cgaggatctg gggaagcgcc
    10381 gcggggagat cgaggcgcag cgggacgccc tcgccaagga cgaggacctc ctgcgtgcgg
    10441 gcatccgcga catcgagcgc gcgctcgagg agacgcaggg cgatgtcgag cgcggacgcg
    10501 agcgtgtcga gcgggccgtc gaggccatcg cccgtgccga ggccaggatt cgcgagcacg
    10561 acgatgtcga gatcaaggcg cgcgaggccc acgctggact cgaggcgcgc cacgccacgc
    10621 tccgggagct caaggagcgc tacgaaggct acgacgcgag cgtgcggtgg ctcgtcgcgg
    10681 ggccgagccg tccgccccgt gtcctcggga ccgtcggcga cgtgctccac gcatccgggg
    10741 actggctccc cgcgctcgaa gccgcgctcg gcgaagcggt ccagttcgtg gtggccgagc
    10801 ggaccgaggc cgcggtccac gcgctccgcg cgctcgagca gagcggcgag ggacgcgcca
    10861 cgttcatctc gctcgaccgc ctctcccgcg tgcacgcggc gcagatctcg gccgaggttc
    10921 tctcggcacc gggcgtgctc gggccgctcc tcgatcacgt tcgcttcgag cccggctacg
    10981 tggcgctcgc ctcgttcctc ctctcgagcg tggtggtcgt ggacacgatc gatgccggcc
    11041 tcgccctgaa cgagcggttc cagggagagc gtctccagtt cgtcacccgc cgcggcgagc
    11101 gggtcgtggg ccctggcatc ttccagggcg gtggcggtcc cacgcgcgcc ggctcggtgc
    11161 tcaggcgcga ggaggagctg acggatctct cggccgagat cgagcgctcc gccgcgagcc
    11221 tcgccgcgat cttgaagacc accgagggga accgcgcgga gcgcgcctcg gccgtgtcgg
    11281 agcaggagga ggcggcgcgc cttctccaag tggccgagca ggcgcaccgc gcgcaggagt
    11341 cccgtctggc ggagcagaag atccgcggcg acgcgatggt gcaggcgtcg tcgtctctcg
    11401 cctcatcgct cggagcggtg gccgcggaac tcgagcgcgc gcttggagag gcggagtcgg
    11461 cgcaggtcgc gctcaagtcc gcggacgagg agcggggccg cgtggacgcg atcctcgcgc
    11521 aggaggagac cgaagtgcgc gagctggagc gcaaccgcga gcaccgcgtg gcgctccacg
    11581 ccgaagcccg cgtcgaagcg acccgggccc gttcctcgct cgaggcggcg attcgcgagc
    11641 gcgagcggct cacccggagc gcggacgagg cggagcggga gatcgggagc cggagggaag
    11701 aggcgatcca gaccgagacg cgcatccagg agaccgagac cctctgcggc gagcgggaga
    11761 cggagctcgc gcaggagatc gagaagaagg ccgagcgcga ggtcctgctc cagcgcgcgc
    11821 gcgagcggtt cggcgaggtg aaggggcagg cggacgacgt cgaatcgacg acccgcgacg
    11881 tccgccgcga gcacgcgacc ttgaccgagc ggctccacaa ggcggagctc gagcaggcgg
    11941 aggcggacgg agagctccgg cggctgctcg agcgcgtgcg gaacgagtac gagatcgacc
    12001 tcgtgtcgtg ggcgccggag gcggaggccg tgccgggcga cagcgccgac cctggcgatc
    12061 ccgcgctcgc cgagatcgag gaaggcgacg tcgagctcga gcccgattcg ccgcgcgtgc
    12121 cggacgaagc gatcgtggac cgcgacgatg cggcgtccgg atccgccccc atcacggccg
    12181 ccgcgacgcg cgaggagcgc gaggcgcggc tccgctacct ccaggagaag ctccgttcga
    12241 tcggtcccgt gaacctcctc gccgtgcagg attacgagga gcggcgccag cgcctcggct
    12301 tcctgaacgg ccagcggaag gatctcgatg acgcgaggca gcagctgctc gaggcgatcg
    12361 agaagatcaa tgccaccgcc tccgagctct tcctcaagac cttcgagcag gtgaacaaga
    12421 acttcgtcca ggtcttcacc acgctcttcc agggcggcga agcggcgctc gtcctgacgg
    12481 gcgatgatcc gctcgaggcg gagatcgaga tcctcgcgcg gccgcgcggg aagaagcccc
    12541 agtccctctc gctcctctcc ggcggcgaga aggcgctcac cgcgatcgcg ctcctcttcg
    12601 cgatctatct cgagaagccg agcccgttct gcatcctcga cgaggtggac gcgccgctcg
    12661 acgacgcgaa catcgaccgc ttcgtcgggc tcctgcgcga cttcagcgcc aagacccagt
    12721 tcatcgtcgt gacgcacaac aagaagacga tggaggtggc ggacgcgctc tacggcgtga
    12781 cgatgcagga gccgggcgtt tccaagctgg tctcggtgcg ctggaaccgc gagcggcagg
    12841 acgtcgacca ggtcgacgct cctcccgagg agctcgcgcc cgccgcctca tagcaggcct
    12901 tgccgtgcag ttcttcaaga agatcaccga ggggctgaag aagacccgcg acgcgttcgt
    12961 cgagcgggtt tccgacgtct tgaagccggg gaagaagctc gagcccgagg actacgaggc
    13021 gctcgaggcg gcgctcctcc gggccgacgt cggccccgcg acctcggatc gcatcctcga
    13081 tgacgtgaag gagcgcctgg cgaaaggcgc cgacgatccg gccttggcgc tccgcgaggc
    13141 cgtccgcgcc gtcgtggagc ctcccgcgcc gtccgccgcg aatgtcccgc gtccccacgt
    13201 caccctcgtc gtcggcgtga acggcaccgg caagaccacc accatcgcga agctcgcgca
    13261 ccgctacaag gaggcgggag aatccgtcct cctcgtggcg tgcgacacgt tccgcgccgc
    13321 ggcgggggat cagctcgagg tctgggccga gcgagctggg gtcgagatgg tgcgccaggc
    13381 atcaggcgcc gaccccgcgg cggtcgcgtt cgacggcatg gcgcgcgcga agagccggaa
    13441 cgttcagcgt gcgatcgtcg acacggcggg gcgcctccac gtgaaggcga atctcatggc
    13501 ggagctggac aagatccgcc gcgtcatcga gcgcggcatt cccggcgcgc cgcacgagac
    13561 cctcctcgtg ctcgacgcga ccgtcgggca gaacggcctt cagcaggcga aggagttcgg
    13621 gcgcatcctt ccgatcacgg ccatcgcgct gaccaagctg gacggcacgg cgaagggcgg
    13681 ggtggtgctc gcgatcgcgg atgcgctcaa gattccgatc cggtgggtcg gcgtgggcga
    13741 gggcatggac gacctcgtgc ccttcgatcc cgaggagtac gctgcggcgc tcacggggag
    13801 ctactcgccc gcgtaggtca cctggttctt gccccgctcc ttcgacaacc gcagatgcag
    13861 attggcgcgc gtccagagat cgtgcgcgag cttctcgcgc tgcccgcgat ccagcccgcg
    13921 cgagcggtcc cacgtgaccg gaacgtccgc ggggaacatc gcgcctccga ggctcatgct
    13981 cacgcgcacc gtggtctcgc gaccgtcgag ccccggcacg gcgaccgcca ggttctccac
    14041 cttgagccgc agacgctcgc agatctcctt ggcctcgagc ttggtcgtct ccgcgctcag
    14101 gatgagcgcg aactcctcgc ctccccagcg cgctacggtg tcgaacggcc ggacgctccc
    14161 cgcgagcgtt cgcccgagct cgcgcaggat cgcgttcccg gcctcgtagc cgagcgtgtc
    14221 gttgacgcgc ttgaagtcgt cgatgtcggc gatcacgagc gacatgggcc gctcctcgcg
    14281 gtaggcgcgc gcgatctcgt gatcgagctg ccgcgcgaag taggggcggt tgtagatgcc
    14341 ggtcagctcg tcgaccatca ccagcatgcc cagcttcttc agcgtgttga ggaggtcggt
    14401 cgcgtgctcg gtgacgatcc cgaggatgcc gaggcgctcc tcgctgaaaa agcgcggagc
    14461 tttgctccac gcttcgagca catagaggaa gggcgtgccg ggctccccca cgcgggccat
    14521 cgccacggag cggaatccgc ccgggacggg gcgcggctcg tagggagcga tggcgacgtg
    14581 gaccgtggcg tcgaccgcct cggtgtcgag catggtctcg atgcgtcccg cctcggccag
    14641 gtcggcgacg tagaccggga gcatgcgctt cccgacccat tccaccgcga gcgccttgtc
    14701 ccacggctcg ccgtcgctca agggctgcgc gtaccgcgcc tcgtcgggcg tcgagccgag
    14761 agggaagagg cgcacgtcgt cgcactcgag cacgtcgcgc gccgaggtga ggatcgtgcg
    14821 caggatgtcg ccgatgtccg tctggagata ctcggtgttc cgctcgtccc gagtgaagag
    14881 cttccgctcc accgcgatca ggtgcgacag gctcttgtag aggagcctgc ttcgcgagtg
    14941 ccagtgcggg cgcaccgcgc gcacgagggt ctcctcgcgg agctcgagcg gggcggggag
    15001 caggaaggtc tcattggtcg ccgggacgcg gtagcgcacg tacggacggc cgtcgcgctg
    15061 gaagcggtcg acgcgctcga gcgatccgcc ctcgaggagc cgctccgcga tctcgccggc
    15121 gagcctgtcg cgagcctcgc cgtcgagatc tgtcaggctg agctcgatga ggtggcgccg
    15181 atcctcggtc gaagcctcgc tgaggcagag ggacgcgagt agggcggttc gaatgcgctg
    15241 cgggtcgttc attcccctcg cctcggacgc gtaacgctcg gatgttgtgg gaaactcgat
    15301 gattatagca catccggcca atttcctgcc gccccccatg cacgggcgcc cgccgaactc
    15361 cgtccgctaa cacgtttttg cgcttgcatt tgagaagcgg ccatgctatt gtctctctcg
    15421 tagccggagt gcatgcccac ggaaagggtg cgcgcatggc ttccaacggt cgtaactcca
    15481 tgcgatcggg tcccatcccc agggtcccca ctcctacgcg agccggcgct cgcggtttcg
    15541 tttccccagt cccccagaag ttgcatgtcg ccggagacac ggaggtggtc tccatgaagc
    15601 gttccagagg caagccccat ccctggccga cctgggtggg tgcggcgctc gggatccttg
    15661 ttttgacgtc ggctccgccg cgaatccagg cgaggccgta caaccccgag tacccgccca
    15721 tgccggacgg cgatccaacc gccgacgacc agccgagccc gacaccaaag ggcggcgggg
    15781 gtcattacgc gacctaccgg aacgatgccg atctgacggc tcgcaagccg ggcgcgacga
    15841 tgaccgcgtc ccagctgcgt ggacgtctga tctggttgtc cttcgttcgt acgtggatct
    15901 ggatcggcat gcgttaacga cgaagcgacc ctggcggcgg ggcgctccgc cgagcgcccc
    15961 cgaggtccca tgagctacga caaggtgccg cgttcggacg tggcttctgt tttacagaaa
    16021 atcgacgacc tcctgtcgcg cgcgcgcttc accgcggcgc tcgaggtcat tgcggggttc
    16081 cgcgccggcc ggagcgttcc ggaggcgctc gataccccgc tcctgctcgc tcggtgccga
    16141 gccctcctcg ggctcgggcg ctggaaggaa gtggctgacg ccgctgaaaa gaagctgagc
    16201 gagctctacg ccctccgtcc cgaggagaag cgatccctgc tcgaattcca tatcgccgcc
    16261 ggtcgcgcgg tgtggaggct gggacgcccc tcacgggcgg aggagcactt ccgtgccgcg
    16321 taccacatca gccgatggga attcgacgac gcggccggca tgctgcgctc gcggaacctt
    16381 ctcggcctct gcttcctcgg cgccggcgag atccatcgcg ccgtcggcga gttcgcgcgc
    16441 ggccagaccc aggcacgcga agctgggctc gtccatgaag aggcctcgtt ctcgctcaac
    16501 ctgagcatcg cgctcggaaa gaacggacgc ctcctcgagg cggacaagga gctcgcccgc
    16561 gcccgaacgc tgttcgccga gcggggccat tcccgcggca aagtgcaggc gcgcctcttc
    16621 cacggctatc tcgcgcgcat tcgtggcgac gcccgcgagg cggagctcca ggcgcagggc
    16681 gcgctctccg aggcggaaga gcacggcttc gagcgggagc gcgtcatcgc gctcgagtat
    16741 ctcggagatc tcgctctcga ccgccacgac aaccaggggg ccgtcgacaa tctggagcag
    16801 gccttcatcc tggccgagcg gatcgcgccc gaaggggacc tcatccccga gctctgccgg
    16861 cgcaccgccg aagtgcacgt ccgtatcggc gagccgaacc gcgcgctcgt cgcctgcgag
    16921 cgcggactcc gggtcgcgcg tgccatccac gaccgcttcg aggaggccgc ggcgcagcgc
    16981 gtcatggcga tggcccacct cctcctcggc catcgcgaga aggcggctcg catcgcacag
    17041 gaaggcgcga tgctcctccg aaagctcgag gccaactacg agctcatgcg gctcctcgtc
    17101 tggacggggg agatgctcgt ggccggaggc gactcggagg agcggcgcga gggccgcgat
    17161 gccctctggg aagcgcggag cctcagcatg gcgatgaagc tcgaccgctg gctcgagcgg
    17221 atcgagcggg atctcggcgt ggagatcgtg cccacggccg ggcccagtcc ggcggatcac
    17281 gccctggatt gcgcgcaccc gtcgggggcg gatcccgact cgtgccgctg ggggatcatc
    17341 acgcaggatc cacgcatgat cgagctcctc gccatcctcg agcgtgcatc ctcgagccgc
    17401 ctcccgatcc tcgtcctggg cgagagcggc acgggcaagg agctcctcgc gcgtgcggcg
    17461 cacggccttt ccgaccgggc cacgcgcccg ttcgtggtcg ggcactgcgc cgcgctctcg
    17521 gacggactcc tggacgtcga gctcttcggc aaggacccgg gcgaggggcc cgggggcgag
    17581 ggtgcgcgca cgggactctt cgagaccgcg agccgcggcg tcatgttcct ggatgaagtg
    17641 ggcgagctcc tcacctccgc gcaggcgaag ctccttcgcg tgatcgagat gggcgagctg
    17701 cgccgcgtgg gtggcatggg cgtgcgccag gtcgatgtgc gggtgatcgt cgcctccacc
    17761 cgcgacctgg gctcgctcgt ccggcgcggc ctcttccggg acgatctcta ctaccggctg
    17821 aacgggattc gcctcgaggt gccgcccttg cgcgagcgcc cgggcgacat cggtctgatc
    17881 ggccggcatc tcctcgaccg cgcctgcttg gaagcgggga agcgcgtgac cttgagcgag
    17941 gaggcgtggg ctcgcctcat cgcgcatccg tggcccggta acgtgagcga gctcaagagc
    18001 gtcatcgagc ggaccgtcgc gctggcgggc gacggcgagc ggctgtccgc cgagcaggtc
    18061 cagttccagc ccgcggtccg cgctccgatc tccgccatga cggaacggac tggaatccgc
    18121 accatgcggc gctcgggcga tccggaggaa cggaaccgga tcgtggacgc gctccgcgcg
    18181 aacggcggca accagtccga ggccgcgcgc agcctgaacg gcatgaagcg gacgacgctc
    18241 ctctacaaga tgaagaagct cgacatccgc cccgaggaat accgtcccgc gcctccgcgc
    18301 tgattgaccc gcccggcgcg cgcccctata ctggcggcga gcacgcgcta accgcgcagc
    18361 atcccggcac ttggcgggcc attggcccca tcccgagcgc cttcccatcc ccgcgaggag
    18421 ccgcatggtt tccgaattga tgaaggggct gtcgatcacg ctgtcccact tcttcaagaa
    18481 gccggtgacg ctcaagtatc cggacgtgaa gatgccgatg tatccgcgct tccgcggact
    18541 ccatgagctc catcgctacg cgaacgggct cgagcgctgc gtctgctgcg gactctgcgc
    18601 ggcggtgtgt cccgccgacg ccatctacat ggaagcggcg gagaacacgc ccacgcagcg
    18661 cttctccgcc ggcgagcgct acgcccggcg ctacgagatc gacatgctcc ggtgcatctt
    18721 ctgcggctac tgcgaagagg cctgccccga ggacgcgatc ttcctgggtc acaacttcga
    18781 gctgtcggac tactcccgcg actcgttcct ctattccaag gagcagctcc tcgtcccggt
    18841 gggcatgacg aacacgccgg gcatcgttcg gggccggtag gcgcgcccgg acgcgatggg
    18901 cgaccgctgg ttgctcgccg cggcctgcgg cgtggtcgcg ggacttgcga acgtcctcgg
    18961 cggctggctc gccgtgggac gccacggctg ggaccgcgtc cggcagagct acttcatggc
    19021 ggccggcgcg ggcttcctcc tcgccgccgt gttcctccgc ctcattcccg agagctttcg
    19081 cctcgcgtcc aatcccaacg gcccgcccac gctcgcggtc ggctcgctca tcctcgtcgg
    19141 atatctcctg atccacgccg ccgagcacgt gctggtgggg cacttcc
//
LOCUS       DASTKC010000121         5428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_324896, whole genome shotgun sequence.
ACCESSION   DASTKC010000121 DASTKC010000000
VERSION     DASTKC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324896"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFR25_05070"
     CDS             <1..913
                     /locus_tag="VFR25_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9940452.1"
                     /translation="DGVFNEWNRYYLSGSAAELLDKLLERDTVILFKKGQKSPWAREF
                     LFADGMTWGAAAPIRQQGERVGALLLNHSASLPFRPEHVATLQNLATLAGIAEREDSH
                     RAHLEDLFMGVIVSLTMALEAKDPYTEGHSARVAAYSEAIGKQLGMQPEQLDVVHRSC
                     LVHDLGKIAVDGNILGKEEGLSSHEREKMDLHPVIGEDILRPITLLKPLLPGVRSHHE
                     HYDGTGYPDGLAGEAIPIEARIMAVADAFDAMTSNRPYRLALPEEDALAELRRNAGTH
                     FDPRVVAAFEQIYPAVKRTLEHLRPRS"
     gene            complement(942..1370)
                     /locus_tag="VFR25_05075"
     CDS             complement(942..1370)
                     /locus_tag="VFR25_05075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940453.1"
                     /translation="MGARPNSSETVHLTIPSRLEELAQVHALLQELGERHGMDEDYVN
                     SLLIAVIEAGTNAVQHGNMFADDKVVKFEVTVDPGNVRVRVDDVGHGFDPSRVLNPTE
                     GEQLLSPHGRGLFLMRQLMDEVKFETGPEHGTTVFLRKAR"
     gene            complement(1458..3713)
                     /locus_tag="VFR25_05080"
     CDS             complement(1458..3713)
                     /locus_tag="VFR25_05080"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET9940454.1"
                     /translation="MNPAPVANASVALAYAMVGVLVVFLGIIILREAPRERANRATAF
                     MLFSGGMGSVLGAIGFIIDILSPSGRTGSNDLLRSFAYLWEFFFPSLLYFACVFPTEN
                     RIFRSIPGATFWIFAPHTFHLILMLLQGQGALWGRVAAGIAKNSLGAAVITYGRLPVE
                     LIFRFHQIMFSMVNLFYIAAALTLLWVSFRKSHNPLIRRQVGTIFAGLGLCAGIYAVA
                     SPIPTLLNHYWPPLMRSSLIILALLIGSGGIAYSMVRHRFLDANLIARKSILYAVTSA
                     FLFGVYVLIVRRFDLLLESVTTADTTIFQTAFLLLSLILFQPVFSWLEEILDRQFLRD
                     RGDYRTLLHRVSGEMLTVLDLDTLAEKLLATLREGVPARTTVLLLAPERRVAVAHGFG
                     GGVDLAAIAACPRDGFVRLVEGAELLRMEEIAPLAQERGVAATMAPFLATGPYLVLPL
                     RHAGQFLGLIALGRKITETRFTAAEVSLLQTLTNQTSVAITNSLLYRDSLDKTILEEE
                     LAVARRIQQQSLPTTLPQTSGFGLAALNAPSKFVGGDYYDTVELGDGHYLMVIADVAG
                     KGVPAALLASMVQASIRTQAFERQSVGVIMNRLNRLVHEATPDDRFATCFLADVDGSG
                     LGNGRGITLSFANAGHNFPILRSAEGHCRSLEQGGIPLGIQPGFLYGHTEASLKPGDS
                     LLLYTDGITDARNRLGEDFGEERLVELVGRLPDRLSADEIVRSVSDEVTRFTDGADQI
                     DDITLLALKAH"
     gene            complement(3710..>5428)
                     /locus_tag="VFR25_05085"
     CDS             complement(3710..>5428)
                     /locus_tag="VFR25_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET9940455.1"
                     /translation="VARLQPPQRGIDLDARRARRSRYVQMDLKGQAVYQKYVYGDANS
                     IAFWPLLETIRKAEEDPTVEGVVLNLSGFQANVAMIWELRQKLADLRARGKKVVVYVD
                     NASVGNFYLASVADRIVMDPMGIVTFPGVQISRTYLKRTLDKLGIGFDEWRWYKYKSA
                     LETFSRTDMSPADREQLQGLVDGAYAELKAGFVSAGRVRTDAFDSVVNDSPVLTARRL
                     KDMGWVDVIGRPDSLKTVVKSLGPKRATLVSPGALDAIRWQPDEEWGPRPEIALVYQI
                     GECAMDEGIKARPTSKQLAKYRKDSGIKAVVVRVDSPGGDALASDLVAGEIRAMHKTT
                     PKTKAKPVIVSQGRVAASGGYWISMDADTIYSTPLSVTASIGVIGGWAWNDGFGKKTG
                     FTSDHVQRGQSADLLGGIRIPLLGATLPERNLTAKERDQVHALFDDFYSGFVTKVATA
                     RHLPETRVRELGEGHVVLGREAQATGLVDRMGSLTDAMEAAKRAAGIKPGQKVRVVEY
                     PKPGLFRLPKFLGGATVLSRGALAPDAPALTYEARVIQEILDRPGQPLLLAPSTLLPA
                     EVEQAR"
BASE COUNT          910 a         1796 c         1860 g          862 t
ORIGIN      
        1 ggacggcgtg ttcaacgagt ggaaccgcta ttacctctcc ggaagcgcgg ccgagctcct
       61 cgacaagctg ctcgagcggg acacggtgat cctcttcaag aaggggcaga agagcccttg
      121 ggcgcgggag ttcctctttg cggacggcat gacctggggc gccgcggcgc ccatccgtca
      181 gcagggcgag cgggtgggcg cgctccttct caaccactcg gcgtcgctgc cgttccgtcc
      241 ggagcacgtg gccacgctcc agaacctggc cacgctcgcc gggatcgccg agcgcgagga
      301 cagccaccgc gcgcatctcg aggacctctt catgggggtc atcgtgtcgc tcacgatggc
      361 gctggaagcg aaggatccgt acaccgaggg gcactccgcg cgcgtggccg cgtactcgga
      421 ggccatcggc aagcagctcg gaatgcagcc ggagcagctc gacgtcgtgc accgctcctg
      481 cctggttcac gacctgggca agatcgcggt cgacgggaac atcctcggca aggaggaggg
      541 gttgagctcg cacgagcggg agaagatgga tcttcacccg gtcatcggcg aggacatcct
      601 gcgccccatc acgcttctga aacccctcct ccctggcgtg cgatcgcacc acgagcacta
      661 cgacggcacg ggctatccgg acggactcgc cggcgaggcg attccgatcg aggcgcgcat
      721 catggcggtg gccgacgcgt tcgacgcgat gacgtcgaac cgcccgtatc gcttggcgct
      781 gcccgaggag gacgccctcg cggagcttcg ccggaacgcg ggcacgcact tcgacccacg
      841 ggtcgtcgcc gccttcgagc agatctatcc cgccgtgaag cggactctgg agcacttgcg
      901 gccgcggagc tgagccgcgg cgtggggcgg gaggcgcgcg cctagcgcgc cttgcggagg
      961 aagaccgtgg tcccgtgctc gggaccggtc tcgaacttca cctcgtccat caattgccgc
     1021 atcaagaaga gcccgcggcc atgcgggctc aggagctgct ccccttcggt cggattgagc
     1081 acgcggctcg ggtcgaatcc gtgtcccacg tcatcgacac gcacgcgcac gttccccggg
     1141 tccaccgtga cctcgaactt cacgaccttg tcgtcggcga acatgttgcc gtgctggacc
     1201 gcattcgtgc cggcctcgat gacggcgatg aggagggagt tgacgtaatc ctcgtccatg
     1261 ccgtggcgct ctcccaattc ttggagtaag gcgtgcacct gcgcgagctc ctcgagccgg
     1321 ctcggaatgg tgagatggac ggtttcgctc gagttggggc gggcgcccat aagatcgcta
     1381 ctgtaccaga atggaccagt ccgggcgcgc cggaatgagg tccgccggag tgcggtcggg
     1441 gcccgtccgg cgcacgctca gtgcgccttc agggcgagca gggtgatgtc gtcgatctgg
     1501 tcggccccgt ccgtgaatcg ggtgacctcg tcggagacgc tcctcacgat ctcatcggcg
     1561 ctcagtcgat cggggagccg gcccaccagc tcgaccagcc gctcttcacc gaaatcctcg
     1621 cccagccggt tccgtgcgtc cgtgatcccg tccgtgtaga ggaggagcga gtcgcctggc
     1681 ttgagcgacg cctcggtgtg cccgtagagg aaccccggct ggatgccgag ggggatgccc
     1741 ccttgctcga gggaacggca gtgtccctcg gccgaacgga ggatcgggaa attgtgcccc
     1801 gcgttcgcga aggacagcgt gatccccctt ccgttcccga gcccgcttcc atccacatcc
     1861 gcgaggaagc aggtggcgaa gcggtcgtcg ggagtagctt cgtgcacgag gcggttgagc
     1921 cggttcatga tcacgcccac cgactggcgc tcgaacgcct gggtgcggat ggaagcctgc
     1981 accatcgacg cgaggagcgc cgcgggcacc cctttgccgg cgacgtccgc gatgaccatc
     2041 aggtagtggc cgtcccccaa ctcaactgtg tcgtagtagt cgccccccac gaacttgctc
     2101 ggcgcgttga gcgcagccag gccgaagccc gatgtctgcg gcagcgtggt cgggagcgac
     2161 tgctgctgga tgcggcgcgc cacggcgagc tcctcctcga ggatcgtctt gtcgaggctg
     2221 tcccggtaga ggagcgagtt cgtgatcgcg acgctcgtct ggttggtgag cgtctggagg
     2281 agcgagacct cggccgcggt gaaccgcgtc tccgtgatct tccgcccgag cgcgatcagt
     2341 ccgaggaact gtcccgcgtg gcggagcggc aggacgagat aggggccggt cgcgagaaag
     2401 ggcgccatcg tggcggccac gccgcgctcc tgtgcgagcg gcgcgatctc ctccatgcgg
     2461 aggagctcgg cgccttccac gaggcgcacg aacccgtcgc ggggacacgc cgcgatcgcc
     2521 gcgagatcca cgccgccgcc gaacccgtgc gccaccgcga cgcgccgctc gggcgcgagg
     2581 aggagcaccg tcgtgcgcgc cggtactccc tcgcggagcg tggcgaggag cttctcggcc
     2641 agcgtgtcga gatcgagaac ggtgagcatc tcgccgctca cccgatggag gagcgtccgg
     2701 taatcgccgc gatcccggag gaactgccgg tcgaggatct cttccagcca tgagaaaacg
     2761 ggctggaaca ggatcagcga aaggagcagg aacgccgtct ggaagatcgt cgtgtccgcc
     2821 gtcgtcaccg actcgaggag gagatcgaag cgccgcacga tcaacacgta cacgccgaac
     2881 aggaaggccg acgtcacggc gtagaggatc gacttccgcg cgatcagatt cgcgtcgagg
     2941 aagcggtggc gcaccatcga gtacgcgatt ccgcccgagc cgatgaggag cgccaggatg
     3001 atcaaggacg agcgcatgag cggcggccag tagtggttca agagggtcgg gatcggcgac
     3061 gcgacggcgt agatcccggc gcacaacccg aggccggcga agatcgtccc gacctgtctc
     3121 cggatgagcg gattgtggct tttccggaag ctcacccaga ggagcgtgag cgcggccgcg
     3181 atgtagaaga ggttcaccat cgagaacatg atctggtgga accggaagat gagctcgacg
     3241 gggagccgcc cgtaggtgat cacggcggcg cctaacgagt tcttggcgat gccggcggcg
     3301 acgcgccccc agagcgcccc ctggccctgg aggagcatga ggatcaggtg gaacgtgtgc
     3361 ggtgcgaaga tccagaacgt cgcgcccggg atgctccgga aaatccggtt ctccgtcggg
     3421 aagacgcacg cgaagtagag gagcgacggg aagaagaact cccagagata ggcgaacgag
     3481 cggaggagat cgttcgagcc cgtgcgcccc gacggcgaga ggatgtcgat gatgaacccg
     3541 atggcgccga gcaccgagcc catcccgccc gagaagagca tgaacgcggt ggcgcggttg
     3601 gcccgctcgc gcggtgcttc acggaggatg atgatcccga ggaacaccac gagcacgccc
     3661 accatggcat aggcgagcgc gaccgaggca ttcgcgacgg gggccggatt catcgagctt
     3721 gttcgacctc cgcgggcagg agcgtgctcg gcgctagaag gagcggctgc cccggccggt
     3781 ccagaatctc ctggatgacc cgcgcttcgt acgtgagcgc gggcgcgtcg ggcgcgaggg
     3841 cgccgcgcga caggacggtc gcgccgccga ggaacttggg cagcctgaag aggcccggct
     3901 tcggatactc gaccacgcgg accttctgcc cgggcttgat gcctgccgcg cgcttggcgg
     3961 cctccatcgc gtcggtgagg ctccccatgc gatccacgag cccggtggcc tgcgcctcgc
     4021 gaccgaggac gacgtgtccc tcgccgagct cgcggacccg tgtctcgggg aggtggcgcg
     4081 ccgtcgcgac cttcgtcacg aagccgctat agaagtcgtc gaacaacgcg tgcacctgat
     4141 cgcgctcctt ggccgtgagg tttcgctccg ggagcgtggc gccgaggagc gggatccgga
     4201 ttccgcccag gaggtcggcg ctctggccgc gctgaacgtg gtcggacgtg aatcccgtct
     4261 tctttccgaa gccgtcgttc cacgcccagc cgccgatcac gccgatcgag gcggtcacgg
     4321 agagcggggt cgaatagatg gtgtctgcgt ccatgctgat ccagtagccg ccgctcgcgg
     4381 ccacgcgtcc ctggctcacg atcaccggct tcgccttcgt cttgggcgtc gtcttgtgca
     4441 tcgcccggat ctcccccgcg acgaggtccg acgcgagcgc gtcgccgccg ggcgaatcga
     4501 cgcggaccac gaccgccttg atgccggagt ccttgcgata cttcgcgagc tgcttcgacg
     4561 tggggcgcgc cttgatgccc tcgtccatgg cgcactcgcc gatctgatag acgagcgcga
     4621 tctccggacg tggcccccac tcctcgtcgg gctgccagcg aatcgcatcg agcgcccccg
     4681 gggagaccag ggtggcacgc ttcggcccaa ggctcttcac cacggtcttg agcgaatccg
     4741 ggcgcccgat gacgtccacc catcccatgt ccttgagccg gcgcgccgtg agaaccggac
     4801 tgtcgttcac cacgctgtcg aacgcgtccg tccgcacgcg ccccgcggac acgaagcccg
     4861 ccttgagctc ggcgtacgcc ccgtcgacca gaccctggag ctgctcccga tcggcggggg
     4921 acatgtcggt gcgcgagaag gtctccagcg ccgacttgta cttgtaccag cgccactcgt
     4981 cgaagccgat cccgagcttg tccagagtcc tcttgagata cgtgcgcgag atctgcaccc
     5041 ccgggaaggt cacgatcccc atgggatcca tcacgatccg gtcggccacg cttgccagat
     5101 agaagttccc cacgctggca ttgtccacgt acacgaccac cttctttcca cgcgcgcgga
     5161 ggtccgcgag tttctgtcga agctcccaga tcatcgcgac gttggcctgg aagcctgaaa
     5221 gattgagcac cactccctcg acggtcgggt cttcctcggc cttgcggatg gtctcgagga
     5281 ggggccagaa cgcgatcgag ttcgcgtctc cgtagacgta cttctggtag accgcctggc
     5341 ccttcaggtc catctggacg tagcggcttc ggcgcgcgcg acgcgcgtcg agatcgatgc
     5401 cgcgctgcgg aggctggagc ctggcgac
//
LOCUS       DASTKC010000122        12788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1034865, whole genome shotgun sequence.
ACCESSION   DASTKC010000122 DASTKC010000000
VERSION     DASTKC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12788
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034865"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1179)
                     /locus_tag="VFR25_05090"
     CDS             complement(271..1179)
                     /locus_tag="VFR25_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET9940456.1"
                     /translation="MLNRRGWELARPFTLIAPALGMFTGSVIALGAAPAVPLTPWVVG
                     RIALGTLMAAVLNAASNTLNQVTDLEADRVNKPGRPIPSGRVTETEALQLAGWLYAAA
                     FVLAALVGPQCTLLAGTAAVLTVLYSAPPFRLKAVPYLANVVIAIPRGVLLKVAGWSC
                     VRDFGRLEPWYIGAIFGLFLLGATTTKDFADIKGDSAAGFRTLPVVHGPRRAAWMITP
                     FLVLPFLLIPYGVHQGYLTGHPWVLYSLTAVLCAWGLYVAYLMVRRPDDLARTENHPS
                     WTHMYVMMFVAQIGFALAYVGRAPGQ"
     gene            complement(1184..3217)
                     /locus_tag="VFR25_05095"
     CDS             complement(1184..3217)
                     /locus_tag="VFR25_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9940457.1"
                     /translation="MPTSRTKQWVVRVLGPVALLAASIAIPLSDAPVRAEPRQRVYIL
                     GFDGMDPEVADRLMAEGKLPNLAWMRDHGAYRRLQTTNPAQSPVAWSAFSTGMNPGKT
                     RIYDFLRRNPSTYYPDFSTVTIQRGKFALGFLPTKAPTVVNNRKGTTFWQIASRNGVR
                     SVILEAPINFPPETLQNGVLLSGLGVPDIRGTMGTFSYWATNATGVGDTEMGGKVARI
                     RVDGTGRVRSVVHGPRNPFASRDAEGRIPDLTIPVEFQRVRGNPGAVKISLQGHVQTI
                     HQGEWSDWYSIQFTIAPLVSVRGIARFHVLEASPELRVYLSPINFDPRKPPVPISQPP
                     SYSGELARRVGLYKTLGWPEDTWALNEEAIDERVFLEDVNFTFNRQRAVVFDAVKQMD
                     PDLFVTVFQSTDKVQHMMWRLIDPKHPMYNRQLAARYGNSIERIYMRADTLVGRFLQK
                     TKDGRTTVMVVSDHGFSSFRKAVNINTWLVRNGYMTLSRTDPVRDRNLEDLFGRGTFW
                     PNVDWSRTRAYALALGQIYVNLRGRERDGIVAPGAEYDALRKELIQKFGALTDPETGE
                     GVVRHVYAREELYRGPYFNEAPDLVVGFERGYRVSWQTSLGGIPPDVFEPNERRWSAD
                     HCSVDPSQVPGVLFSSRPLDKKDARIIDLAPTVLTRLGLPVPVDMDGADLGLR"
     gene            3294..7610
                     /locus_tag="VFR25_05100"
     CDS             3294..7610
                     /locus_tag="VFR25_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HET9940458.1"
                     /translation="MTLFESAPYNPATVRSHHPRGTTLIGSRRIAPNVRAFNVALLVA
                     APLLLSLALVAPRRASAAAPIAAPGASSPERVRTLAQDIRGATFEYVPGEIRFDTLSV
                     NGAGYSRVTVAGATVGEKPGDPALPTATLYVGVPDGMSPKLTIVSEEWNERAGPAPPV
                     PVQHQRFISDDPAHGPVTEFRVVPNPDVYGKAAIFPSRSAYLTRGGAVGEMWVVPIQV
                     RPVRWDPSARAFRLLRRMSLRVDFVPATAAERSKRPAFRQGGDSGFWGRVQKGLIRNY
                     ESARSFPIRPAAPRPAAPRRSAVNPEFKLQVTQTGWTSVSYATLSAAGFPPGKTIATI
                     GVSQRGYDDGADAPTDTPVAVVARDANGNGTFDTGDAITFYAQSLRDRLGPASIENRY
                     TAVNVYWLTWSGTNAAAADSVSGAIPGSPITPPSFLDTIHLEQNKFLLAAPNPISTSP
                     PEAVPYIFWTRGDDLENNDIFDTPIPFVDPDAAQPFRIRSYYQGQNNFQHHLNLFYLS
                     STNVTDTLGLNRTFFHQDTYVLDTGFTIPGGHIGPNTGPGNRYRHVGTREPPDGGNIV
                     PGSRAWLDWVEVTYSRLYRAVSNSLSFGSGTALGLVEMTVTGFTAPGIQVYDVTDPLA
                     PKRVTNLTIGGTGPYSVTFRTDASAGERRFVAVVPGSEITLGSGLVAQDNPSNLSVPD
                     PFPSTSVARSIIIAPESFITASNAPVTRLADYRRGQGYVVQVAGVQDVYDEFNGGLKS
                     PLAIRRYLRHAYMAWTPRLLYALLIGDASMDYQHQLPTSSADLLPTYIKFEAIFGPLG
                     QELVSNESWYSRNLNLAVPVDGEDLPSIVLSRIPAGTPDDLDLFVNKVIAYENFQPTD
                     TWRARMLLASDDAFSTGLFSSTGYCYNFQEEDFLSANQTFGLIAANSTGGQDMTPDYY
                     DLHTYTDAVPSNPPQFGCKSLTSVVNALGQTGGGYSAMTSKLTQGGLILNIETHANRY
                     VIGHEVIYCTGSPRGGCPTGSPGPQDFNNVGRPFFGMVWGCHSNQFADGPALAEGGID
                     SSDAIGEQWLLLNNRGTIGSVGSTAFELLPQNTNFSHSVADAFFTTPPASDPGPGSPR
                     QVRWVLGEVFTSALVMNGLSFDPSQSIMNHTVNLLADPMLRMDALPPRVFDVQVDGST
                     ISDGGALVSDAPTGGAQVIAKLRDEVAIQSVQLTEQDLVGGPAVPIDSTSYTVAYTDS
                     SRQATLTTSIRPRVGNYDVQIRATDLNGRAQLFTLEVRTPVHYFANGTQIVNGVFVAS
                     GSRIRADITTPVPVTADSLELFLDGAPISAAKTGAGRQWSLDALPGFGPGTHTLQIAV
                     GGRTAGLELVTFQVSAEFTMRGVAVVSPKIQGAGCGGSIFQYELSSSADKVELLLMTV
                     AGRRVSSIRVPGNAGFNVYCWDGRDSQGHDTAIGVYLFRIRATDPTGRTVTQDGRMIR
                     TR"
     gene            7607..8578
                     /gene="murQ"
                     /locus_tag="VFR25_05105"
     CDS             7607..8578
                     /gene="murQ"
                     /locus_tag="VFR25_05105"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET9940459.1"
                     /translation="MSDRRSGKAIFKELSKLVTEQRNPRSRRLDRLSTPQVLRLMNSE
                     DRRVPLAVGREIPRISKAVDLIVARLERGGRLFYVGAGTSGRLGVLDASECPPTFGTP
                     RTMVQGIIAGGRRALVRSIEGAEDDAQAAVAALKKRRVGEKDIVVGIMASRRTPYPLG
                     AIAYGRKVGAATIAVTANPGRGMDIDCDVVIAPRVGPEILTGSTRLKSGTAQKLVLNM
                     LTTATMVRMGKVYENLMVDLKTASRKLEERTKRVLMHATGATYEETDAWLERAGGSLK
                     VAIVMKRAGVTRREAERRLKEAQGWVRGALSEPRAARLGRKGGRGMR"
     gene            8575..9795
                     /locus_tag="VFR25_05110"
     CDS             8575..9795
                     /locus_tag="VFR25_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9940460.1"
                     /translation="MRRAALLAVAFISFALASGCAKSPKNVLVFWQFSPITAIQPVLE
                     RFEAQHPGVKVQVEQLTWQSGREKIVAAIAAGRPPDLCELGSTFLPGLVADSTLIDLT
                     DRVGDLRPDLVGWNVSSYKGRVYGIPWMLGTRALFVNDNLVNEAKMDPGAPIVTWDDL
                     RAKVQAISERCPGARGFGMNAGEREILFKKFMPFAWGNGGDILDSTMTKSVMGSPQNI
                     EALKFYLSLKPYSLLDRQEMHEQAFIKGRLGVVISGPWLIRKLAQEAPKLVYSVQLMP
                     QPAKDRGTHASFAGAEVLGIFRGSKKKDEALELARFLVKPENTMPVYIATGNAFPAAI
                     SVGTDPYFDRFPLDAKFLDQLKTAVGPPLHPKWVQIEEIVNGELEEAIYGKKTAEEAL
                     RSADQRIDAVLAAP"
     gene            9792..10664
                     /locus_tag="VFR25_05115"
     CDS             9792..10664
                     /locus_tag="VFR25_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9940461.1"
                     /translation="MRRVTGVQGLAPWGLFFAAFGIFPLVYALALSFLAYNPLRPDLV
                     RWVGFDNYARALSSPSFWHALRTTVVFVVGTLPVTLTLAYLVSGLLASVRRGQGFFRA
                     AIFFPATLSMVVISLVFKSLYAEEGLLNGWLAALGLSRVHWLQDPALALPSIMLMDIW
                     ASVGYYAILILAARKTLPEEQFEAARLEGLTRLQIERRIVLPHVRPVLLFVVLLNTIR
                     SFQIFIEVFVLTRGGPLESTLTLVYHLYERAFYHFEMGYASALAYLLLIVVGAILIVQ
                     RRLLGGDPMEARAR"
     gene            10690..11505
                     /locus_tag="VFR25_05120"
     CDS             10690..11505
                     /locus_tag="VFR25_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9940462.1"
                     /translation="MKRSSTLAYVGLGAVALVSLAPLLYMARVSLGQGGDLPVTPGDW
                     LAQPFSLEHYRTILTGGPMARFALNSIVVTAVAVPLQILFAAAAGHALARVRFIGRGV
                     FIALATAFLILPRQVTLVPLYLLLSRAGLLDTYLALILPHLADPFGVLFMAHYYQTMS
                     PELEEAARTDGWSSVATFWRIVLPLSGPGLAVVGVNGFLITWNAFLHPLVLTTSESMR
                     TLPVGLALLAQSEYAVDWGALLAGSTLATLPVVVAFAFFQRQIVEGVLKGSGR"
     gene            11502..12671
                     /locus_tag="VFR25_05125"
     CDS             11502..12671
                     /locus_tag="VFR25_05125"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET9940463.1"
                     /translation="MSRSDLVGRARAVGIMSGTSSDGADAAVVEIDRGGARRQTRLLS
                     FASRPYGDELRHRVLGAQEGTLPLRELFRVHVEVAEVASAAARDALAQAPAGPPPEVV
                     GYHGQTVFHDPHGERSGKRVTVQIGEPAVLAAALGVTIVSNFRMADLLAGGEGAPLVP
                     LFDYHQLASDTRDRVLVNLGGIANLTRLRAGAPLDETLAFDCGPGNMLLDGIVGALGP
                     AGSRYDAGGALAKPGHVAEQLLRYFLSEPFFERTPPKSAGREDFGAGYLSRFIARTDG
                     LSLADRLRTACALTAAAVARGIEQSGPGRPDEVFVSGGGARNATLIEEIEARTRIPVA
                     STEALGVPSDAKEAMAFAFLAFESLRGRPGNVPSATGAKQAVVLGSFTPPPGGRR"
BASE COUNT         2023 a         4369 c         4293 g         2103 t
ORIGIN      
        1 gcccacccga cccctacctc atcgcgaagg acctcgccgg agcaccggag tgccgtgact
       61 tgcgtccgga tgcggcgacc gagcgaattg tgacgccgct taggcttttc tacgactggc
      121 tgtgtgcgcc gcgccagtag ccctttcgtc atggtagggg ctggggtcgt gatggctgga
      181 gggcgcctcg cgagcttcgc gcgaagcgcg aagcaatgcg ccgccgcccg gaggccatca
      241 cgactccagc cccgggccaa gccaaaagag ctactgcccc ggagcgcgcc ccacgtaagc
      301 caacgcaaat ccgatctgcg cgacgaacat catcacgtac atatgcgtcc agctcggatg
      361 gttctcggtg cgcgcaagat cgtctgggcg gcggaccatg aggtacgcga cgtagagtcc
      421 ccaggcgcag aggacggccg tgagcgagta gagcacccag ggatgcccgg tcagatagcc
      481 ctggtgtacg ccgtagggga tgaggaggaa ggggagaacc aagaaaggcg tgatcatcca
      541 ggcggcgcgg cgcgggccgt ggaccacggg gagcgtgcgg aacccggcgg ccgagtcccc
      601 cttgatgtcc gcgaagtcct tggtcgtggt tgcgccgagg aggaagagcc cgaagatcgc
      661 gccgatgtac cagggctcga ggcgtccgaa gtcccgcacg caggaccagc cggctacctt
      721 caaaagaacg ccgcgcggaa tggcgatcac cacgttcgcg agatagggaa ccgccttcag
      781 gcgaaacggt ggggccgagt agagcaccgt gagcactgcg gccgtccctg cgaggagcgt
      841 gcactgcggg ccgacgagcg cggcgagcac gaacgcggca gcgtagagcc agcccgcgag
      901 ctggagcgcc tccgtctcgg tcacgcgccc cgaagggatg ggccgccctg gcttgttcac
      961 gcggtcggcc tcgagatcgg tgacctggtt gagcgtgttc gaggcggcgt tcaggaccgc
     1021 ggccatcagc gttccgaggg cgatccggcc cacgacccag ggcgtcaggg gcaccgccgg
     1081 agcggcgccg agcgcgatga cgcttcccgt gaacatgccg agcgcggggg cgatgagcgt
     1141 gaaggggcgc gccagctccc acccgcggcg gttcagcatg tgttcagcgg agaccgagat
     1201 ccgccccgtc catgtccacg ggaacgggaa gaccgagccg cgtcaggacg gtgggggcga
     1261 ggtcgatgat gcgggcgtcc ttcttgtcga gcggacggct cgagaagagc acgccgggaa
     1321 cctgcgatgg gtccaccgag cagtggtcgg ccgaccagcg gcgctcgttc ggttcgaaca
     1381 catccggcgg gatgccgccg agtgatgtct gccaggacac gcgatagccc cgctcgaatc
     1441 ccacgacgag atcgggcgcc tcgttgaagt agggcccacg gtagagctcc tcgcgagcgt
     1501 agacgtgccg gaccacgccc tctcccgtct cggggtccgt gagggcgccg aacttctgga
     1561 tgagctcctt ccggagcgcg tcgtactccg ctcctggcgc cacgatgccg tctcgctccc
     1621 ggcctctcag gttcacgtag atctggccca gcgcgagcgc gtacgcgcgc gtcctcgacc
     1681 agtccacgtt gggccagaac gttccgcgtc cgaagagatc ctcgagattc cggtcccgca
     1741 cgggatcggt cctggacagg gtcatgtagc cgttccgcac gagccacgtg ttgatgttca
     1801 cggccttccg gaagctcgag aagccgtgat cggagacgac catcacggtc gtccgtccat
     1861 ccttggtctt ctggaggaac cggccgacga gcgtatccgc cctcatgtag atccgctcga
     1921 tcgagttgcc gtagcgcgcg gcgagctggc ggttgtacat cggatgcttc gggtcgatga
     1981 gccgccacat catgtgctgg accttgtccg tggactggaa caccgtgacg aagagatcgg
     2041 gatccatctg cttcacggcg tcgaacacga ccgcgcgctg acggttgaac gtgaagttca
     2101 cgtcctcgag gaacacgcgc tcgtcgatgg cctcctcgtt caaggcccac gtgtcctcgg
     2161 gccagccgag cgtcttgtag aggccgacgc ggcgcgcgag ctcgccgctg taggacggcg
     2221 gctgcgagat gggcaccgga ggcttgcgcg gatcgaagtt gatcggggag agatagaccc
     2281 ggagctcggg cgaggcctcg agcacgtgga agcgggcgat gccgcgcacc gagacgaggg
     2341 gtgcgatcgt gaactggatc gagtaccaat cgctccactc gccctgatgg atggtttgca
     2401 cgtgtccctg gagcgagatc ttcacggcgc ccggattgcc acggacgcgc tggaactcga
     2461 ccgggatcgt caggtcgggg atccgtccct cggcatcgcg cgaggcgaag gggttccgcg
     2521 gcccgtgcac caccgagcgc acccgcccgg tcccgtcgac ccggatccgg gccaccttgc
     2581 ctcccatctc cgtgtccccg acgccggtcg cgttcgtggc ccagtaggag aaggtgccca
     2641 tggtgccccg aatgtccgga accccaagcc cggagaggag tacgccattt tgtagcgtct
     2701 ccggtgggaa gttgatcggg gcttccagga tgaccgagcg cacgccgttc cgggacgcga
     2761 tctgccagaa cgtcgttccc ttgcgattgt tgacgacggt cggcgccttc gtgggcagga
     2821 agccgagagc gaatttcccc cgctggatcg tcacggtgga gaagtccggg tagtaggtgc
     2881 tcggattccg tctcaggaag tcgtagatcc gggtcttgcc cggattcatg ccggtcgaga
     2941 aggccgacca ggccaccggc gactgcgccg gattcgtggt ctgaagccgc cggtaggcgc
     3001 cgtggtcccg catccatgcg aggttgggga gcttcccctc cgccatgagg cggtccgcga
     3061 cttcgggatc catcccgtcg aaaccgagga tgtagacgcg ctggcgtggc tcggcacgga
     3121 cgggagcgtc ggagagggga atagcgatcg aagcggcgag gagcgcgacc ggccccagca
     3181 cccgcacgac ccactgtttg gttcgggagg ttggcattcg tggataccgg cgcgggaatc
     3241 gtagcatcgc gattcacgga gtgtcaaacg agccgagcgc gcgggaggtt gccttgacgc
     3301 tcttcgagag cgctccctat aatccggcca ccgtccgttc ccaccatcca cgagggacaa
     3361 cgctcatcgg ctcccgacgc atcgccccga acgtccgcgc attcaacgtc gcgttgctcg
     3421 ttgctgcgcc gcttctactc tcgctcgccc ttgtcgctcc caggcgcgcc tcggccgcgg
     3481 ccccgatcgc ggcgcccggc gcgtcgtcgc ccgagcgcgt gcgcaccctg gcgcaggaca
     3541 tccggggggc gaccttcgag tacgtccccg gtgagatccg cttcgacacg ctgagcgtga
     3601 acggagccgg ctattcccgg gtgacggtcg cgggcgcgac ggtcggagag aaacctgggg
     3661 acccggccct cccgacggcg acgctctacg tgggggtgcc cgacggcatg agccccaagc
     3721 tcaccatcgt gagcgaggag tggaacgagc gcgccgggcc ggcgcctccc gttcccgtcc
     3781 agcaccagcg cttcatcagc gacgatcccg cgcacggtcc cgtcaccgag ttccgggtcg
     3841 tgccgaaccc ggacgtctac ggcaaggccg cgatcttccc ctcgcgctcg gcctacctca
     3901 cgcgcggcgg agccgtgggc gagatgtggg tcgtgccgat ccaggtgcgg ccggtgcggt
     3961 gggatccatc ggctcgcgcc ttccgccttc ttcggcggat gagcttgcgc gtggacttcg
     4021 tgcccgcgac cgcggcagag cgctcgaagc gccccgcatt ccgccagggc ggcgactccg
     4081 gcttctgggg gcgcgtccag aagggactca tccggaatta cgagtccgcc cggtcgttcc
     4141 ccatccggcc cgcggcgcca cggcccgcgg ctccacggcg ctctgccgtg aatccggagt
     4201 tcaagctgca ggtgacccag accggatgga cttcggtgag ctacgcgacg ctctcggcgg
     4261 ccggatttcc cccgggaaaa acgatcgcca cgatcggcgt cagccagagg ggctacgacg
     4321 acggggccga cgcgcccacc gacacgccgg tcgcggtcgt cgcgcgggac gcgaacggaa
     4381 acggaacctt cgacacaggg gacgcgatca cgttctacgc gcagagtctt cgcgatcgtc
     4441 tcggcccggc ctccatcgag aaccgctaca ccgcggtcaa cgtgtactgg ctcacatgga
     4501 gcgggaccaa cgcggccgcc gccgattccg tgtccggcgc catccccggg agccccatca
     4561 cgccgccgtc gttcctcgac acgatccacc tcgagcagaa caagtttttg ctcgccgctc
     4621 ccaaccccat ctcgacgtcg ccccccgagg cggtcccgta catcttctgg acccgcggtg
     4681 acgacctcga gaacaacgac atcttcgaca cgccgatccc gttcgtcgat ccagatgcgg
     4741 cgcagccgtt ccggatccgc tcgtactatc aggggcagaa caatttccag caccatttga
     4801 atcttttcta tctgtcgtcg acgaacgtca ccgacacgct cggattgaac cggacgttct
     4861 tccaccagga cacctacgtc ctcgatacgg gattcacgat ccccggaggc cacatcgggc
     4921 cgaacacggg accgggcaat cgctaccggc acgtcgggac gcgcgaaccg cccgacggcg
     4981 gcaacatcgt gccgggcagc cgggcgtggc tcgattgggt cgaggtcacc tattcccggc
     5041 tctaccgtgc cgtgagcaat tcgctctcgt tcggaagcgg cacggcgctc ggactggtcg
     5101 agatgaccgt cacgggcttc accgctccgg gaatccaggt ctatgacgtg accgatcctc
     5161 tcgcccccaa gcgcgtgacg aatctcacga tcggcggaac gggcccgtac agcgtcacgt
     5221 tccgcaccga cgcatccgcg ggagagcgcc gcttcgtggc ggtcgtgccg gggagcgaga
     5281 tcacgctcgg atcgggcctg gtcgcgcagg acaacccgtc caatctgagc gtcccggatc
     5341 cgttcccttc cacgagcgtc gcgcggtcga tcatcatcgc gccggagtcc ttcatcaccg
     5401 cgtcgaacgc gccggtgacg cgcctcgcgg actatcgccg gggccagggc tacgtggtcc
     5461 aggtcgcggg agtccaggat gtgtacgacg agttcaacgg cgggctgaag tcgcctctcg
     5521 cgatacgccg ctatctgcgc cacgcgtaca tggcctggac gcccaggctc ctctacgcgc
     5581 tcctgatcgg cgacgcgagc atggattatc agcatcagct cccgacgagc agcgccgatc
     5641 tcttgccgac gtacatcaaa ttcgaagcga tcttcggtcc cttggggcag gagctggtct
     5701 cgaacgaatc ctggtattcg cgcaacctga acctggccgt gcccgtcgat ggagaagacc
     5761 ttccgagcat cgtcctctcg cggattcccg ccgggacccc cgacgacctg gatctcttcg
     5821 tgaacaaggt gatcgcctac gagaacttcc agcccaccga cacatggcgg gcgcggatgc
     5881 tgctcgcgtc ggacgatgca ttctccacgg gactcttctc gtccacgggc tattgctaca
     5941 acttccagga agaggatttc ctgagcgcga atcagacgtt cggcctcatc gcggccaaca
     6001 gcaccggagg tcaggacatg accccggatt actacgatct gcacacctac accgacgccg
     6061 tgccgtcgaa ccctcctcag ttcgggtgca agagcttgac ctcggtcgtc aacgcgcttg
     6121 gccagacggg cggcgggtac tccgcgatga cctccaagct cacgcagggg gggctcatcc
     6181 tgaacatcga gacgcacgcg aatcgatacg tcatcgggca cgaggtgatc tactgcaccg
     6241 gctcaccgcg aggagggtgc cccaccggca gtcccgggcc gcaggacttc aacaacgtcg
     6301 gacgtccgtt cttcggcatg gtctgggggt gccactccaa tcagttcgcc gacggaccgg
     6361 ccctggccga gggcggcatc gacagctcgg acgcgatcgg cgagcagtgg ctgctcctca
     6421 acaaccgagg cacgatcgga tcggtgggca gcaccgcgtt cgagctcctg ccccagaaca
     6481 cgaacttcag ccattcggtg gcggatgcgt tcttcaccac cccgccggca tcggatccag
     6541 ggcccgggag cccgcggcag gtccgttggg tgctggggga ggtctttacg agcgccctgg
     6601 tgatgaacgg gctctcgttc gaccccagcc agtcgatcat gaaccacacg gtcaaccttc
     6661 tggccgaccc catgcttcgg atggacgccc taccgccgcg cgtcttcgat gtgcaggtgg
     6721 acggctcgac gatctcggac ggcggggcgc tcgtgagcga tgcccccacg ggcggcgcgc
     6781 aggtgatcgc gaagctccgg gacgaggtcg ccatccagtc ggtccagctc accgagcagg
     6841 atctggtcgg agggccggcc gtgccgatcg actcgacgag ctataccgtc gcctacaccg
     6901 actcgagccg gcaggccacg ctgacgacct cgatccgtcc gcgcgtcggc aactacgacg
     6961 tccagatccg cgcgacggac ctgaacggcc gcgcccagct cttcacgctc gaggtgcgga
     7021 cgccggtcca ctacttcgcg aacggcaccc agatcgtgaa cggggtcttc gtggcgagcg
     7081 ggtctcgaat ccgcgcggat atcacgacgc cggttccggt caccgccgac tccctggagc
     7141 tcttcctcga cggagcgccg atctctgcgg cgaagaccgg agcggggcgg cagtggtcgc
     7201 tcgacgcgct tccaggattc ggcccaggga cgcacacgct ccagatcgcg gtcggaggac
     7261 gcaccgcggg gctcgagctc gtgaccttcc aggtgagcgc cgagttcacc atgcgcggcg
     7321 tcgcggtcgt gagtcccaag atccaggggg cgggctgtgg cggatccatc ttccagtacg
     7381 aactcagctc ctcggcggac aaggtggagc ttctgctcat gaccgtggcc gggcgtcgcg
     7441 tctcctccat ccgcgtgccc gggaacgccg gattcaacgt ctactgctgg gacggccgcg
     7501 attcgcaggg tcacgacacc gcgatcggcg tctatctctt ccgaatccgc gcgacggatc
     7561 cgaccgggcg cacggtgacc caggatggcc gcatgatccg cacgcgatga gcgatcgccg
     7621 gagcgggaag gccatcttca aggagctctc gaagctcgtc acggagcagc gcaatccgcg
     7681 ctcacgccgc ctcgatcggc tctcgacgcc tcaggtgctg cggctgatga attccgagga
     7741 ccgccgcgtt cccctggcgg tcgggcgcga gattcccagg atctcgaagg ccgtggacct
     7801 gatcgtggcg cgtctcgagc gcggggggcg cctcttctac gtcggcgccg gcacgagcgg
     7861 gcggctcggc gtgctcgacg cgtcggagtg ccctcccacc ttcggcacgc cgcgcacgat
     7921 ggtgcaggga atcatcgccg gcgggcgccg cgcgctcgtc cgctcgatcg aaggggccga
     7981 ggacgatgcc caagccgccg tggcggcgct caagaagcgc cgcgtcggcg agaaggacat
     8041 cgtggtcggg atcatggcga gccgccgcac gccgtacccg ctcggcgcca tcgcctatgg
     8101 ccggaaggtg ggagccgcca ccatcgcggt cacggccaat ccgggccggg gcatggatat
     8161 cgactgcgac gtggtgatcg cgccgcgcgt gggcccggag atcctcacgg gatcgacgcg
     8221 gctcaagtcc ggtaccgcgc agaagctcgt cctcaacatg ctcaccaccg cgaccatggt
     8281 gcggatgggc aaggtctacg agaacctcat ggtcgatctc aagaccgcga gccgtaagct
     8341 ggaggagcgg acgaagcgcg tgctcatgca cgcgaccggc gcgacgtacg aggagaccga
     8401 cgcctggctc gagcgggccg gaggctcgct caaggtggcg atcgtgatga agcgcgcggg
     8461 cgtgacgcgc cgggaggcgg agcggagact caaggaggcc cagggatggg tgaggggagc
     8521 tttaagcgag ccgcgcgcag cgcggctcgg aaggaagggt gggaggggaa tgcggtgaga
     8581 cgcgcggccc tgctcgccgt cgcgttcatt tccttcgccc tcgcgtccgg ctgcgcgaag
     8641 tcccccaaga acgtcctcgt cttctggcag ttctctccga tcacggccat ccagcccgtc
     8701 ctcgagcgtt tcgaagcgca gcaccccggc gtcaaggtcc aggtggagca gctcacctgg
     8761 cagagcgggc gcgagaagat cgtggccgcg atcgccgcgg gacgcccccc ggatctctgc
     8821 gagctgggct ccacgttcct ccccgggctc gtggccgaca gcacgctcat cgatctcacg
     8881 gatcgggtcg gcgacctccg cccggatctc gtcggctgga acgtgtccag ctacaaggga
     8941 cgggtgtacg gcatcccctg gatgctcggg acgcgcgcgc tcttcgtgaa cgacaacctc
     9001 gtgaacgagg ccaagatgga tccgggcgcg ccgatcgtca cgtgggatga cctcagggcc
     9061 aaggtccagg cgatctcgga gcgctgcccg ggcgcgcgcg gattcgggat gaacgcgggc
     9121 gagcgcgaga tcctgttcaa gaagttcatg ccgttcgcgt gggggaacgg cggagacatc
     9181 ctcgactcca cgatgacgaa gagcgtgatg gggagcccgc agaacatcga ggccttgaag
     9241 ttctacctct cgctcaagcc ctactcgctg ctcgaccgcc aggagatgca cgagcaggcc
     9301 ttcatcaagg ggcggctcgg cgtcgtcatc tcgggtccgt ggctgatccg gaagctcgcc
     9361 caggaggcgc ccaagctcgt ctactcggtg cagctcatgc cgcagccggc gaaggacaga
     9421 gggacgcacg cgtccttcgc gggggcggag gtgctcggga tcttccgcgg gtcgaagaag
     9481 aaggacgagg cgctcgagct ggcgcgcttc ctcgtcaagc cggagaacac gatgcccgtc
     9541 tacatcgcga ccgggaacgc gttccccgcg gcgatctccg tcggcaccga tccctacttc
     9601 gaccgctttc ccctcgacgc gaagttcctg gaccagctca agaccgcggt cggcccgccg
     9661 ctccatccga agtgggtgca gatcgaggag atcgtgaacg gcgagctgga agaggcgatc
     9721 tacggcaaga agacggccga ggaggcgctt cggtccgccg accagcgcat cgacgcggtg
     9781 ctcgcggctc cgtgaggcgg gtcaccggcg tccagggcct cgcgccgtgg gggctcttct
     9841 tcgccgcgtt cggaatcttc ccgctcgtct acgccctcgc gctcagcttt ctcgcgtaca
     9901 acccgctccg ccccgatctc gttcgctggg tgggcttcga caactacgcg cgcgccctgt
     9961 cgtccccgtc gttctggcac gcgctccgga ccacggtggt cttcgtggtg ggaaccctgc
    10021 ccgtgacgct cacgctcgca tacttagtct ccgggctcct cgcgagcgtg cggcgcggtc
    10081 aggggttctt ccgcgccgcc atcttctttc ccgcgaccct gtcgatggtc gtgatctcgc
    10141 tcgtgttcaa atcgctgtat gcggaggagg gcctcctgaa cggctggctc gccgctcttg
    10201 ggctctcgcg cgtgcactgg ctccaggatc ccgcgctcgc gctcccctcg atcatgctca
    10261 tggacatctg ggcgagcgtc ggctactacg cgatcctgat cctcgccgcg cgaaagacat
    10321 tgcccgagga gcagttcgag gcggcgcggc tcgagggact cacgcggctc cagatcgagc
    10381 gtcgaatcgt gctgccgcat gttcgccccg tgctcctctt cgtcgtgctc ctcaacacga
    10441 tccggtcgtt ccagatcttc atcgaagtgt tcgtcctcac gcgcggcggc ccgctcgaga
    10501 gcacgctcac gctcgtctat cacctctacg agcgcgcgtt ctatcacttc gagatgggct
    10561 acgcgtcggc gctcgcctat ctgctcctca tcgtcgtggg ggcgatccta atcgtgcaaa
    10621 ggcggcttct cggtggggat cccatggagg cgcgggcgcg atgatggcgt gggctctggg
    10681 agctgcggcg tgaagcgctc gtccacgctc gcctacgtcg ggctcggcgc ggtcgcgctc
    10741 gtctcgctcg cccccctcct ctacatggcg cgcgtctccc tggggcaggg aggcgatctc
    10801 ccggtgacgc cgggggactg gctcgcgcag cccttctcgc tcgagcacta ccggaccatc
    10861 ttgacgggcg gcccgatggc gcgattcgcg ctcaactcga tcgtcgtgac ggcggtggcg
    10921 gtgccgcttc agatactctt cgctgccgcg gccgggcacg cgctcgcgcg ggtacggttc
    10981 atcgggcggg gcgtcttcat cgccctcgcg accgcgtttc tcatcctgcc ccggcaggtc
    11041 acgctcgtgc cgctctatct cctcctctcg cgtgcggggc tcctcgacac gtatctcgcg
    11101 ctgatcctgc cgcacctggc cgatccgttc ggcgttctct tcatggcgca ttactatcag
    11161 accatgtcac ccgagctgga agaggcggcg cgcacggatg ggtggtcctc cgtcgcgacc
    11221 ttctggcgca tcgtgctgcc tctttccgga ccggggctcg cggtcgtggg cgtgaatgga
    11281 ttcctgatca cctggaacgc gttcctccac ccgctcgtgt tgacgacatc cgagtccatg
    11341 cgcacgctcc cggtggggct cgcccttctt gcgcagtcgg aatacgcggt cgactggggc
    11401 gcgctccttg cgggctcgac cctcgccacg ctgcccgtcg tcgtcgcgtt cgccttcttc
    11461 cagcgccaga tcgtggaggg cgtcttgaag gggagcggcc ggtgagccgt tccgatctcg
    11521 tcgggcgggc gcgagccgtg ggcatcatga gcggaacgtc ctcggatggc gcggacgccg
    11581 cggtcgtcga gatcgatcgc ggcggcgcgc gccgccaaac caggctcctc tcgttcgcat
    11641 cgcggcccta cggcgacgag ctcaggcatc gcgtgctcgg cgcgcaggag ggcaccctcc
    11701 ccttgcgcga gctcttccgc gtgcacgtcg aggtggccga ggtcgcgtcc gcggcggcgc
    11761 gggacgccct cgcccaggcg ccggccggcc cgccgcccga ggtcgtcggc taccacgggc
    11821 aaaccgtatt ccacgatcca cacggagagc gctcgggaaa gcgggtcacc gtgcagatcg
    11881 gggagcccgc cgtgctcgcg gccgcgctgg gtgtgacgat cgtctcgaac ttccggatgg
    11941 ccgatctcct cgcgggcggc gagggagcgc cgctcgtgcc gctcttcgac tatcaccagc
    12001 tcgcgtcgga cacgcgggac cgcgtgctcg tcaatctcgg aggcatcgcg aatctcacgc
    12061 gccttcgcgc gggagcgccg ctcgacgaga ctctcgcctt cgattgcggc ccgggcaaca
    12121 tgctcctcga cggcatcgtc ggcgcgctcg gtcccgcggg atcgaggtac gacgccggtg
    12181 gcgcgcttgc caagccaggg cacgttgccg agcaattgct ccgctacttc ctctccgagc
    12241 cctttttcga gcgcacgcca ccgaagagcg cgggtcgcga ggacttcggc gcgggctatc
    12301 tctcgcgctt catcgcccgc acggatggcc tctcgctcgc cgaccgcttg agaaccgcct
    12361 gcgcgctcac ggcagccgcg gtggcgcgcg ggatcgagca gtctggacca ggaagaccgg
    12421 acgaggtctt cgtgtccggc ggtggcgcga ggaacgcgac cctgatcgag gagatcgaag
    12481 cccgcacccg catcccggtc gcgtcgacgg aagctctggg cgtgccgtcc gacgcgaagg
    12541 aggcaatggc cttcgcgttc ctcgcgttcg aatccctccg cggacggccg ggcaacgtgc
    12601 cgtcggcgac gggagcgaag caggcggtgg ttctggggtc cttcacgccg cctcccgggg
    12661 gtcgacgatg aaccgcagga cggcccgacc ctggaccgcg tggctcgtct tcctggcttt
    12721 ctcggggagc ctccttcagg ccggctgcgc ggggtccagg tccgagccac gacccgagcc
    12781 agggcccg
//
LOCUS       DASTKC010000123         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_89899, whole genome shotgun sequence.
ACCESSION   DASTKC010000123 DASTKC010000000
VERSION     DASTKC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89899"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1229
                     /gene="hemE"
                     /locus_tag="VFR25_05130"
     CDS             204..1229
                     /gene="hemE"
                     /locus_tag="VFR25_05130"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET9940464.1"
                     /translation="MKNDHFLRALMRLPTPYTPIWIMRQAGRYLPEYRALREKHSFLD
                     VCRVPELCAEVTAQPIDRFGFDAAILFQDILIPLLGLGVPLDFDPGPRLGLSVTSRED
                     VDRLHWQGMDAIPHIRPVVRAVRQRLAGSVPLIGFAGAPFTVAAYLVEGGGARDLSKT
                     RGFLARDSDAFARLLDLLTTATIDYLKAQIGAGVDAVMLFDTQAGWLPPAEFARTAAA
                     SADRVLRALPKGTPTIYFALAPSVGHLEALRAVHADVFGLDFRVSLAQARAILGNGKA
                     VQGNLDPATLLGPGEEIVVGAEAVLRENRGRPGHIFNLGHGIFPDTPVENVKLLVDTV
                     KRYRARG"
     gene            1237..2628
                     /gene="hemN"
                     /locus_tag="VFR25_05135"
     CDS             1237..2628
                     /gene="hemN"
                     /locus_tag="VFR25_05135"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET9940465.1"
                     /translation="MSRTTAQITAELLAKYDRPGPRYTSYPTAIEFNESFTPERYLEK
                     LDLAARHPDEPLSLYVHLPFCRERCSFCGCNVIITQKPGVAGAYLDDLEREVALVAAR
                     LGKRTKVVQYHWGGGTPTYLDLDQMRRVWRLFADRFSFQPGAEIAIEVDPRVTTRRQA
                     ELLKELGFNRISMGVQDFDSDVQEAVNRYQTFNQTRDLHLYLRELGFESINFDFIYGL
                     PHQTPVTFRKTIELALELRPDRVACYSYAFVPWIKAHQKAIRLEDLPPRDVKLELFGI
                     AYELFTGAGYQQIGMDHFAVESDSLAVAAREHTLFRNFMGYTTHPAKDSVGFGVSAIG
                     DLGGAFAQNTKKLNRYKDALDLGLPPIERGFERSKDDEIRRDVIQSLMCNFHLDIRAL
                     ERAYGIDFASYFADSLRRLDEGPGANGFVTRTADAIHVTETGRLFVRNVCMEFDAYLP
                     GRETEKPIFSRTV"
     gene            2640..3587
                     /gene="hemH"
                     /locus_tag="VFR25_05140"
     CDS             2640..3587
                     /gene="hemH"
                     /locus_tag="VFR25_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET9940466.1"
                     /translation="MLLNLGGPDRLDAVRPFLVRLFSDREIIRLPGGPIGQFVIGRMI
                     AYSRTKEVQENYHRIGGGSPIVRWSTLQGRGMVERLRKRGHDVEFALAMRYWNPTTAD
                     ALDELEGKKVDRLLALTLYPHYSVATTGSSVAELERVMKRRGTKLPLDVIDAWHDQPT
                     YIRAMAERAKAALAKMPADAKPTILVSAHGLPQHFIDDGDPYCGHIKTCMDAILAQLP
                     PLPSVLAYQSRVGPVPWIGPSTDEMINRLAKEGVRDVVVIPISFVSDHVETLYEIDLL
                     YGEQAKKLGFRTFARAESLNDYPLFLDALAELVEPRLRG"
     gene            3584..>4981
                     /gene="hemG"
                     /locus_tag="VFR25_05145"
     CDS             3584..>4981
                     /gene="hemG"
                     /locus_tag="VFR25_05145"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET9940467.1"
                     /translation="MKVAILGAGIAGLSTAHFIRREAARRSLPLELLVYEAGARAGGR
                     IRTQEDQGYRLEWAANGIQGTDGAASRLADSLGLKSEKVVARPDAARRYILKRGRLHL
                     LPVSPPAILGFRALSARGKLRLAAEPFMARRASKDESVHAFAARHIGEEAASTLVGTF
                     VRGIFAGDATKLSLDASFPLMREMERKHRSLVLAMMQGKRNPHSRTLWSLKSGMESLV
                     RGLSAEVGPGLRLGAPALALARLADGVRSKWQIRFASGEEAQADAVVIASPSHAAAAL
                     LRSLEPEIARPLAALPCAGLAVVGLGFRPEAFRRAPDGYGFLVAPGDEPGVLGALCET
                     NLWPERAPEGRILIRAMVGGVGAESVVTRSDAELVGLAMKALDRAWGLSGGPERSWVV
                     RQMDAIPQYPVGHRQALGTIAGRLDLWPGLYLTGSAYRGVSVASLIEDAERIAARLTE
                     RAAKAGCRAKITRAGA"
BASE COUNT          775 a         1744 c         1682 g          780 t
ORIGIN      
        1 ctcgcggccg cgtggggcgg gcctcatgag gaggtcgtat tcgaggggaa gggccacaac
       61 gacctctacg ggcccgaatt cgaggccgcg gtccggggat tcctcgacaa gcacctgtaa
      121 aatgacggca ctgtcgatgt agacggcagc gtgaacggcg ttggagcgga cgagaagcat
      181 cgccgctcaa cgccagcgag cacatgaaga acgatcattt tctaagggcg ttgatgcggc
      241 ttccgacgcc gtacacgcct atctggatca tgcgccaggc cgggcgctat ctccccgagt
      301 accgggcgct ccgcgagaag cactcgttcc tcgacgtctg ccgggttccg gagctctgcg
      361 ccgaggtcac cgcgcagccg atcgaccggt tcgggttcga cgcggcgatt ctcttccagg
      421 acatcctgat tccactcctc ggcctgggcg tgcccctgga tttcgatccc ggtccgcggc
      481 tcgggctcag cgtcacaagt cgcgaggacg tggaccgcct ccactggcag ggcatggacg
      541 cgattcccca cattcgtccc gtggttcgcg ccgtgcgtca gcgcctcgcc gggagcgtgc
      601 cgctcatcgg cttcgcgggg gcgccgttca ccgtggccgc ctatctcgtc gaggggggag
      661 gcgcgcgcga tctctcgaag acccgcggct ttctcgcgcg ggattccgat gccttcgcgc
      721 gactcctcga tctcctcacc accgcgacga tcgactatct caaggcgcag atcggagcgg
      781 gcgtggacgc ggtgatgctc ttcgatacgc aggcgggatg gcttccgccg gccgagttcg
      841 cgcgcaccgc ggccgcttcg gccgaccgcg tgctccgggc gctcccgaag ggaacgccga
      901 ccatctactt cgcgctggct ccctcggtcg ggcatctcga agcgcttcgc gccgtgcacg
      961 ccgacgtgtt cgggctcgac ttccgggtct cgctcgcgca ggcacgcgcc atcctcggca
     1021 acgggaaggc ggtccagggg aacctggacc ccgccacgct cctcggcccg ggggaggaga
     1081 tcgtggtggg agccgaggcg gtgctgcggg agaatcgagg gcggccgggg cacatcttca
     1141 atctggggca cgggatcttc cccgacaccc ccgtcgagaa cgtgaagctc ctcgtggata
     1201 ccgtgaagcg ctaccgcgcg cgcggatagg aaggacatga gccgcaccac agcccagatc
     1261 accgccgagc tcctggccaa gtacgaccgg cccgggccgc gctacacgtc gtatccaacg
     1321 gcgatcgagt tcaacgagag cttcacgccg gagcgctatc tcgagaagct cgacctggcc
     1381 gcgcgccacc cggacgagcc gctttccctc tacgttcacc ttcccttctg ccgggagcgc
     1441 tgcagcttct gcgggtgcaa cgtgatcatc acgcagaagc cgggcgtggc gggcgcgtat
     1501 ctcgacgacc tcgagcgcga ggtcgcgctc gtcgccgcgc ggctcggaaa gcggacgaag
     1561 gtggtgcagt accactgggg cggcggcacg cccacctatc tcgacctcga ccagatgcgc
     1621 cgggtgtggc gcctcttcgc cgaccggttc tccttccagc ccggcgccga gatcgcgatc
     1681 gaggtggatc cgcgcgtcac gacccggcgc caggcggagc tcctgaaaga gctcggcttc
     1741 aaccgcatct cgatgggggt ccaggatttc gattccgacg tgcaggaggc ggtgaaccgc
     1801 taccagacgt tcaaccagac gcgcgatctc catctctacc tgcgcgagct cggattcgag
     1861 tcgatcaact tcgatttcat ctacggcctc ccgcaccaga cgccggtgac gttccggaag
     1921 acgatcgagc tggcgctcga gctccgcccg gaccgcgtgg cctgctactc ctacgcgttc
     1981 gtgccgtgga tcaaggcgca ccagaaggcg atccggctcg aggacctgcc gccgcgcgac
     2041 gtgaagctcg agctcttcgg cattgcctac gagctcttca ccggcgccgg ctaccagcag
     2101 atcgggatgg atcacttcgc ggtggagagc gactcgctcg ccgtcgccgc gcgcgagcac
     2161 acgctcttcc ggaacttcat ggggtacacg acgcaccccg ccaaggactc cgtcggcttc
     2221 ggcgtctcgg cgatcggcga tctcgggggc gcgttcgcgc agaacacgaa gaagctgaac
     2281 cgctacaagg acgccctcga cctggggctc ccgcccatcg agcgcgggtt cgagcgttcc
     2341 aaggacgacg agatccgccg ggacgtgatc cagagcctga tgtgcaactt ccatctcgac
     2401 atccgggctc tcgaacgcgc ctacgggatc gacttcgcct cctacttcgc cgactcgctc
     2461 cgccgcctcg acgagggtcc gggcgccaac ggcttcgtga cgcgcaccgc ggacgcgatc
     2521 cacgtcacgg agacaggacg cctcttcgtc cggaacgtct gcatggaatt cgacgcctat
     2581 cttcccggcc gcgagacgga gaaaccgatc ttctcgagga ccgtctgaag accggaattc
     2641 tgctcctcaa tctgggcggt cccgatcggc tcgacgccgt gcgccccttc ctcgtccgcc
     2701 tcttcagcga ccgcgagatc atccggcttc ccggcggtcc catcgggcag ttcgtgatcg
     2761 gacgcatgat cgcgtacagc cggaccaagg aagtgcagga gaactaccac cggatcggcg
     2821 ggggctcgcc gatcgtgcgc tggagcacgc tccaggggcg cggcatggtc gagcggctga
     2881 ggaagcgcgg ccacgacgtc gagttcgccc tcgccatgcg ctactggaac ccgaccaccg
     2941 ccgacgcgct ggacgagctc gaagggaaga aggtggaccg gctcctcgcg ctcacgctct
     3001 acccgcacta ctcggtcgcc accacgggct cgagcgtggc ggagctcgag cgcgtgatga
     3061 agcggcgcgg aacgaagctc ccgctcgacg tgatcgacgc ctggcacgac cagccgacct
     3121 acatccgggc gatggccgag cgcgccaagg cggctctcgc gaagatgccc gcggacgcga
     3181 agcccacgat cctcgtgagc gcgcatggac tgccgcaaca cttcatcgac gacggcgatc
     3241 cctactgcgg gcacatcaag acgtgcatgg acgcgatcct ggctcagctc cccccccttc
     3301 cgagcgttct cgcctaccag agccgggtcg gccccgtgcc ctggatcggc ccgagcaccg
     3361 acgagatgat caaccggctc gcgaaggagg gcgtgcggga cgtggtcgtg atcccgatca
     3421 gcttcgtctc cgaccacgtc gagacgctct acgagatcga tctcctctac ggcgagcagg
     3481 cgaagaagct tggattccgc acgttcgcgc gcgcggaatc gctcaacgac taccccctgt
     3541 tcctggacgc gctcgccgaa ctggtcgagc cccggctccg cggatgaagg tcgcgatcct
     3601 gggcgcgggg atcgcggggc tctcgaccgc ccatttcatc cgccgggagg cggcgcggcg
     3661 gagcctgccg ctcgagctcc tcgtctacga ggcgggcgcg cgcgccggcg gccgcatccg
     3721 gacgcaggag gaccagggct atcgcctcga gtgggccgcc aacggcatcc aagggaccga
     3781 cggcgccgcc tcgaggctcg ccgactcgct gggactcaag agcgagaagg tcgtcgcgcg
     3841 ccccgatgcg gcgcggcgct atatcttgaa gcgaggaagg ctccatctgc tccccgtctc
     3901 gcccccggcg atccttggct ttcgggccct ctccgcgcgc ggcaagctcc gcctcgccgc
     3961 cgagcccttc atggcgcgtc gcgcctccaa ggacgaaagc gtgcacgcgt ttgccgcgcg
     4021 ccacatcggc gaggaggccg cgtcgacgct cgtgggcacg ttcgtgcggg gcatcttcgc
     4081 cggggacgcg acgaagctct cgctcgacgc ctcgttcccc ctgatgcgcg agatggagcg
     4141 gaagcaccgc tcgctcgtcc tcgcgatgat gcagggaaag cgaaacccgc actcacggac
     4201 gctctggagc ctcaagagcg gcatggagtc gctcgtgcgc gggctcagcg cggaggtcgg
     4261 gccggggctc aggctcggcg cccccgcgct cgccctcgcg cggctcgcgg acggcgtgcg
     4321 atcgaagtgg cagatccggt tcgcgagcgg agaggaggcc caggcggacg cggtggtgat
     4381 cgcgtccccc tcccacgccg cggccgccct cctccgctcg ctcgagccgg agatcgcgcg
     4441 ccctctggcc gccctcccct gcgcgggact tgcggtcgtg ggcctcggct ttcgtcccga
     4501 agcgttccgg agggcgcccg acggctacgg atttctcgtc gctcccggag acgagcccgg
     4561 cgttctgggc gcgctctgcg agacaaatct atggcccgag cgcgctccgg aggggcggat
     4621 cctgatccgc gccatggtgg gaggcgtcgg cgcggagagc gtggtcacgc ggagcgacgc
     4681 cgagctcgtg gggctggcga tgaaggcgct cgatcgcgcg tgggggttga gcggcggacc
     4741 cgagcgctcg tgggtcgtcc gtcagatgga tgcgatcccg cagtatccgg tcggccatcg
     4801 gcaggcgctc ggcacgatcg cgggacggct cgatctctgg ccgggcctct atctcacggg
     4861 gagcgcgtat cgcggagtgt cggtcgcgtc cctgatcgag gacgcggagc ggatcgcggc
     4921 gcggctcacg gagcgcgcgg ccaaggctgg ttgtcgcgcg aagatcacgc gcgcgggcgc
     4981 t
//
LOCUS       DASTKC010000124         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1037561, whole genome shotgun sequence.
ACCESSION   DASTKC010000124 DASTKC010000000
VERSION     DASTKC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037561"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFR25_05150"
     CDS             complement(<1..235)
                     /locus_tag="VFR25_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9940468.1"
                     /translation="MLRSIATTLALALTVAAAPASESTYGTITGRVVGSDTKNGIAYV
                     AVMIMDAQTGGQTDREGRFSIIHLQPGTYEMRLR"
     gene            complement(244..1338)
                     /gene="rho"
                     /locus_tag="VFR25_05155"
     CDS             complement(244..1338)
                     /gene="rho"
                     /locus_tag="VFR25_05155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9940469.1"
                     /translation="MATTCSGVLEILPRGGGFLRDPAQSFQQSRDDAFVPDALIRRYG
                     LVEGAFVGGVSQPGRQGPQLADVELISGLTPEAWRARTPFAKLVATSPNRRFRLSDSG
                     NATMRIIDLFAPVGRGTRGLIASPPKAGKTQLLEDFAKAIHADAPETRIVVLLVDERP
                     EEVTHFRRQVPVEVIASSSDQGTAEHVRLANLCMAQVRCELEAGHHVVVLVDSLTRMG
                     RAFNVRGSDSGRTMSGGIDARALEIPRQFFGMARNVENGGSVTVLATALIETGSRMDD
                     LIFEEFKGTGNSEIVLDRALADQRVFPAINIAKTGTRRDELLYSPTELAAINRMRRQA
                     IEMPPKQAILDLIKVLETFPTNEDLLKRVG"
     gene            complement(1389..2738)
                     /locus_tag="VFR25_05160"
     CDS             complement(1389..2738)
                     /locus_tag="VFR25_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940470.1"
                     /translation="MTSPPEGARRGGLSFERRLLIWALLTGLPGTALALVLLWAGHLD
                     VRLQWSGTILLVILWTALAVRLRERVVRPVQTLSNMMSALLEGDYSIRTRDTRPEDAL
                     GLAFREINALGQTLREQRLGALDASALLRKVMEEIDAAVFVFDHEGRLRLVNRAGERM
                     LGRPSERLLGLTAHDLALDGALGTEGPQTIDALFPGRAGRWEARSTLFRQGGLPHRLL
                     MMTDLSRALREEERLAWQRLVRVLGHEINNSLTPIMSIAGSLRDRLKRDERSEDLEAD
                     LDRGLEVIVGRTEALRRFMSAHARLARLPSPRLAPLDVETWIRRVVALETRQLVEVAP
                     GPTIVLHADGDQLDQVLINLVRNAVEASTETGGGVRVGWKRDGNAVCVRVEDDGPGLP
                     QSANLFVPFFTTKPNGTGIGLVLSRQIAEAHGGSLTLEGRDVARGTAAVLRLPFEGT"
     gene            complement(2722..4101)
                     /locus_tag="VFR25_05165"
     CDS             complement(2722..4101)
                     /locus_tag="VFR25_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9940471.1"
                     /translation="MKPVNLNGSAILVADDQADVLEALRLLLKGEGFRVETASSPAGV
                     LRALGEREFDAILMDLNYARDTTSGREGLDLLAELRTLDPTVPVVVMTAWGSIEGAVE
                     AMRHGARDYVEKPWDNTRLLRTLRTQVDLGRALRRSERLESENRILRQEGAPELIADS
                     RAMQPVLNLLRRVGPSTANVLITGEHGTGKEVLARWLHELSPRADRPLVTVNAGGLAE
                     GVFESELFGHVKGAFTDARSDRIGFFEMADGGTLFLDEIGNLKPGQQAKLLRVLQTGE
                     LTRVGSSRTKQVDVRVLAATNIDIRREVTDGRFREDLLYRINTVEIHLPPLRERAEDI
                     PRLAASFLARATTRYGKRIGGFTPEALLIMAAHAWPGNVRELEHAVERAVLLASDDAR
                     IGPADLLLRPAAEGSGRLEDMTLDEVERHLIRRALDRYEGNVSRAADALGLSRSALYR
                     RLQQHDLTP"
     gene            complement(4134..>5560)
                     /locus_tag="VFR25_05170"
     CDS             complement(4134..>5560)
                     /locus_tag="VFR25_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9940472.1"
                     /translation="AALIGVHAALAGGLAASLGFRMLVHALPLGALSGAAHLDWSLYT
                     AAIVVAMLAALATALIPAIVVWRGDLRGAMTHVRTGGLSARGAKLEGSLVVAQIALAV
                     LLGAGAGLLIRSVANLHRIEPGVRVDRVAVIDAAMPTQLSIDDRHRSVLDLLPALQAL
                     PRVRSVAATQKLPLRGTGDSWGIGIEGKPDLPSSTTYMRLVTQDYFKTLGVPIRKGRD
                     FGSSDRDDAPRVTIINEALAAKYFPNEDPLGRIINTGFEGGERIVGVVANVAEAELTD
                     APAPARYMLYGQTPFVPQQISYVLAADRPDAVEGVLREAHATVERRGRNLAVQDAMTM
                     QAVLDRAVGAPGRVAILLSLLAGLALVLGSIGVYGMISQFVSRRTRDYGVRIALGLTR
                     GGVISHVLGRGLKLVALGCAFGIGAAMVLTRLLSSLLYGVPAADLPAFAAAVLALVLV
                     GTVAALVPARRASRTDPVAALRQE"
BASE COUNT          864 a         1905 c         1950 g          841 t
ORIGIN      
        1 tcctcaaccg catctcatag gtgccgggtt gtagatggat aatgctgaag cgtccctcac
       61 gatccgtctg ccctcccgtc tgggcatcca tgatcatgac cgcgacatag gcgataccgt
      121 tcttggtatc cgagcccacg actcggccgg ttatcgtgcc gtaggtcgac tctgaagcag
      181 gagctgcggc aaccgtgagg gccaaggcta gggttgtggc gatcgagcga agcatggctg
      241 agctcacccc acgcgtttca gcaaatcctc gttggtcggg aaggtctcca gcaccttgat
      301 caggtcgaga atcgcctgct tgggcggcat ctcgatcgcc tggcggcgca tgcggttgat
      361 cgcggcgagt tccgtggggg agtagaggag ctcgtcccgg cgggtgccgg tcttcgcgat
      421 gttgatcgcg gggaacacgc gctgatcggc gagcgcgcgg tccagcacga tctcgctgtt
      481 tcccgtcccc ttgaactcct cgaagatcag atcgtccatc cgcgagcccg tctcgatgag
      541 cgcggtcgca agaacggtta ccgagcctcc gttctccacg tttcgagcca tgccgaagaa
      601 ctggcgcggg atttcgagcg cgcgcgcgtc gatcccgccg ctcatcgtgc gccctgagtc
      661 ggagccccgc acgttgaacg cgcgccccat gcgcgtgagg ctgtccacca agaccaccac
      721 gtggtggccc gcttcgagct cgcagcggac ctgggccatg cagagattgg ccaggcggac
      781 gtgctcggcg gtgccctgat cgctgctgct cgcgatcacc tccacgggga cctgacggcg
      841 aaagtgagtc acttcctcgg gccgctcgtc gacgagcaac accacgatcc tcgtctcggg
      901 tgcgtcggcg tggatcgcct tggcgaaatc ctcgaggagc tgggtcttgc cggccttggg
      961 aggagaggcg atcaggccgc gcgtgccgcg tcccacggga gcgaacaggt cgatgatgcg
     1021 catcgtggcg ttgccggagt cgctgagacg aaagcggcga ttcgggctgg tcgcgacgag
     1081 cttcgcgaag ggcgtgcgcg cgcgccaggc ctcgggggtg agaccggaga tcagctcgac
     1141 gtccgcgagc tgcgggccct ggcgtccggg ctggctcaca ccgccgacga acgccccctc
     1201 gaccaggccg tagcggcgga tcagcgcgtc gggaacgaac gcgtcgtcgc ggctctgctg
     1261 gaacgattgg gcggggtcgc gcaggaagcc gcccccgcgg ggcaggattt cgagtactcc
     1321 cgaacaggtc gtggccatcg gtcccgccaa ggatacatgg tctagggccc gtgtcgacag
     1381 gagcgagctc aggttccttc gaacggcagt cggaggacgg ccgcggtacc ccgcgcgacg
     1441 tcgcgccctt cgagcgtgag cgatccgccg tgggcttcgg cgatctgacg gctcaagacg
     1501 aggccgatcc cggtcccgtt cggcttcgtc gtgaagaacg ggacgaagag attcgccgac
     1561 tgaggaagcc cgggtccgtc gtcctcgact cggacgcaga cggcgttgcc gtcgcgcttc
     1621 catcccaccc gcacgccgcc tcccgtctcg gtggaggctt cgaccgcgtt ccggaccagg
     1681 ttgatgagca cctggtcgag ctgatcgccg tcagcgtgaa gcacgatcgt cggtccgggc
     1741 gcgacctcga ccagctgcct cgtctcgagt gcgacgacgc gacggatcca ggtctccacg
     1801 tcgaggggtg ccaggcgggg tgacgggagc cgtgcgagcc gcgcgtgcgc gctcatgaag
     1861 cggcggagcg cttcggtgcg cccgacgatc acctcgagcc cccgatcgag gtccgcttcg
     1921 agatcctccg accgctcgtc ccgcttcagc cgatcgcgaa gactccccgc gatcgacatg
     1981 atcggcgtga gcgagttgtt gatctcgtgt ccgagcaccc tcacgagacg ctgccacgcg
     2041 aggcgctcct cctcgcgaag ggcgcggctc aggtccgtca tcatgaggag ccggtgcggc
     2101 agcccaccct gtcggaacag ggtcgaccgc gcctcccagc gccccgcacg accgggaaag
     2161 agcgcgtcga tcgtttgcgg tccctccgtt ccgagcgctc cgtccagcgc caaatcgtgt
     2221 gcggtcagtc ccaggagccg ctccgacggc cgcccgagca tgcgctcgcc cgcgcgattc
     2281 acgagacgca gccggccctc gtggtcgaag acgaagaccg ccgcgtcgat ctcctccatg
     2341 accttgcgca agagcgcgct cgcatcgagc gcgccgagtc tctgttcccg gagagtctgg
     2401 ccgagcgcat tgatctcgcg gaacgcgagc ccgagcgcgt cctcggggcg ggtgtcccgc
     2461 gtgcggatgg agtagtcccc ttcgaggagc gcggacatca tgttggagag cgtctgcacg
     2521 ggccggacca cgcgctcccg gaggcgcacc gcgagcgcgg tccagaggat gacgagcagg
     2581 atcgtgccgc tccactgaag gcggacgtcg aggtggcccg cccagagcag aacgagcgcg
     2641 agggcggtgc ccggaagtcc ggtgaggagc gcccagatga ggagccggcg ctcgaagctc
     2701 aggccgcccc ggcgcgcccc ctcagggggt gaggtcatgc tgctgcagcc tgcggtagag
     2761 cgcgctccgg ctcagcccga gcgcgtcggc ggcgcggctc acgttccctt cgtagcggtc
     2821 gagcgcgcgg cggatgaggt ggcgctccac ctcgtccaga gtcatgtcct cgagccgccc
     2881 ggagccctcc gccgcagggc ggagcagaag atccgcgggc ccgatgcgcg cgtcgtccga
     2941 ggccaggagc accgcgcgct ccacggcgtg ctccagctcc cgcacgttcc cgggccacgc
     3001 gtgcgccgcc atgatcaaga gcgcttccgg cgtgaagccc ccgattcgct tcccgtaccg
     3061 cgtcgtggcg cgggcgagga acgacgccgc gaggcggggg atgtcttcag cccgctcgcg
     3121 aagcggtgga agatggatct cgaccgtgtt gatccggtag aggagatcct cgcggaatcg
     3181 tccgtcggtc acctcgcgcc ggatatcgat gttcgtcgcc gccagcactc gcacgtcgac
     3241 ctgcttcgtg cgcgaggagc cgacccgcgt gagctcgccc gtctggagca cgcggaggag
     3301 cttggcctgc tgtcccggct tgagattccc gatctcgtcg aggaagagcg tcccgccgtc
     3361 cgccatctcg aagaatccga tccggtcgct ccgcgcgtcc gtgaacgccc ccttcacgtg
     3421 gccgaagagc tcgctctcga acacgccttc cgcgagtccg cccgcgttca ccgtgacgag
     3481 cggccggtcc gcgcgcgggc tcagctcatg cagccatcgc gccagcactt ccttgcccgt
     3541 cccgtgctca ccggtgatga gcacgttggc ggtggacggt ccgactcgcc ggagcagatt
     3601 gaggacgggc tgcatcgcgc gggagtcggc gatcagctcg ggagcgccct cctgacgcag
     3661 gattcggttc tcgctctcga ggcgctcgct ccgtcgaagc gcgcgtccca ggtccacctg
     3721 cgtgcgcagc gttcgcagga ggcgcgtgtt gtcccacggc ttctcgacgt aatcgcgcgc
     3781 accgtggcgc atcgcctcga ccgcgccctc gatgctgccc catgcggtca tcacgacgac
     3841 cggcaccgtg ggatcgagcg tgcgcagctc ggcgaggaga tcgagccctt cgcgccccga
     3901 ggtcgtgtcg cgcgcgtagt tcaggtccat gaggatggcg tcgaactccc gctccccgag
     3961 ggcccgaagc acgcccgccg gcgagctcgc ggtctcgaca cggaacccct cacccttcag
     4021 gagcagacgg agggcttcga ggacgtcggc ctggtcgtcg gcgacgagaa tggcggagcc
     4081 gttcaggttc acgggcttca tggtagctca gccccggtcg gagagcagcg gcgctactcc
     4141 tggcgcaagg cggcgaccgg atcggtccgg ctcgcccggc gagcggggac cagagccgcg
     4201 accgtgccca cgagcacaag ggcgaggacc gcggccgcga aggcggggag atccgccgcc
     4261 gggacgccat agagaaggga cgaaagaagc cgcgtgagga ccatggcggc gccgatcccg
     4321 aacgcgcagc cgagcgcgac gagcttcaat ccgcgtccga gcacgtgcga gatcacgccc
     4381 ccgcgcgtga gcccgagggc gatccggacg ccgtagtcgc gcgtccggcg cgacacgaac
     4441 tgcgagatca tgccgtacac gccgatcgac ccgagcacga gcgcgagccc ggcgaggagc
     4501 gagaggagga tcgcaacgcg cccaggcgca cccacggcgc ggtcgaggac cgcctgcatc
     4561 gtcatggcgt cctgcaccgc caggttgcgt ccccgccgct ccaccgtcgc gtgcgcttcg
     4621 cgcagcacgc cctccaccgc gtcgggtcgg tccgcggcga gaacgtagga aatctgctgg
     4681 ggcacgaacg gggtctgccc gtagagcatg tagcgcgcgg gagcgggtgc gtcggtcagc
     4741 tcggcttccg ccacgttggc gaccacgccg acgatgcgct ctcctccctc gaatccggtg
     4801 ttaatgatcc ggccgagcgg gtcctcgttc gggaagtact tcgcggcgag agcttcgttg
     4861 atgatggtga cgcgcggcgc atcgtctcga tccgacgatc cgaaatcacg tcccttccgg
     4921 atcgggacgc cgagcgtctt gaagtagtcc tgggtgacga ggcgcatgta cgtcgtgctc
     4981 gagggaaggt cgggctttcc ttcgatcccg atgccccacg aatcgccggt cccgcggagc
     5041 ggaagcttct gcgtcgccgc gacggagcgc acgcggggga gcgcttgcag cgcgggcagg
     5101 agatcgagca cagagcgatg gcggtcgtcg atcgagagct gggtcggcat ggcggcgtcg
     5161 atgacggcga ctcgatcgac ccggacaccc ggctcgatcc ggtgcaggtt cgccacgctc
     5221 cggatgagga gccctgcgcc cgcgccgagc aggaccgcca gcgcgatctg cgcgacgacg
     5281 aggcttcctt cgagcttggc gccgcgcgcg gagaggccgc ccgtgcgcac gtgggtcatc
     5341 gcgccgcgca gatcgccgcg ccagaccacg atcgccggaa tgagcgcggt cgcgagcgcg
     5401 gcgagcatgg ccaccacgat cgccgccgtg tagagcgacc agtcgagatg cgccgcgccc
     5461 gagagcgctc cgagcggcag ggcgtggacg agcatccgga atccgagcga cgcggcgagg
     5521 ccgccggcga gtgcggcgtg cacgccgatg agcgccgcct
//
LOCUS       DASTKC010000125         8607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1037673, whole genome shotgun sequence.
ACCESSION   DASTKC010000125 DASTKC010000000
VERSION     DASTKC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037673"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1584
                     /locus_tag="VFR25_05175"
     CDS             43..1584
                     /locus_tag="VFR25_05175"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9940473.1"
                     /translation="MDTLKYLEDLERRAELGGGEERQRRQHEAGKLTARERIEVLLDP
                     GSFVEMDKFVTHRCADFGMEQQKILGDGVVTGYGTVAGRQVFVFAQDFTVFGGTLSEE
                     NAKKICNVMDLALENGAPIIGLNDSGGARIQEGVQSLGGYAEIFLRNTIASGVVPQIS
                     AIMGPCAGGAVYSPAITDFVLMVQGTSHMFVTGPNVIKTVTNEDVSFEDLGGAMSHNT
                     KSGVAHFAVEDDEACLAAIRRLLSFLPQNNVDDPPRLASNDPPERMDMKLAEIVPAEP
                     NKPYDVKDIIRLVVDDADFFEVHEHYARNIVVGFARLDGRSIGVVANQPAALAGVLDI
                     NSSTKGARFVRFCDAFNIPLVTFEDVPGFLPGTQQEWGGIIRHGAKLLFAYCEATVPK
                     LTVVLRKAYGGAYDVMSSKHIRGDYNVAWPGAELAVMGAEGAVNIVYRKEIDGAKDPA
                     KARAKLIEEYNDKFANPYVAAALGYLDDVIEPQETRPRLIRALRMLAHKRQTLPPKKH
                     GNIPL"
     gene            1597..3168
                     /locus_tag="VFR25_05180"
     CDS             1597..3168
                     /locus_tag="VFR25_05180"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9940474.1"
                     /translation="MPTKPIRAVLVLNRGEIAIRICRTLREMGIRSIAVFSDADRDAP
                     HVFAADAAYRLGPAAASQSYLNGAKVIETARAARADAVHPGYGFLAESADFAEMVEKA
                     GLVWIGPTPAATRALGDKVSARAIAINAGVPVLPGTEGPVSDPAELRRAADSIGYPVV
                     LKAAAGGGGKGMRRVNAAGEFEQAVRLTQGEAKGAFGDDRLYLERWVERPRHIEVQIL
                     ADRHGTVLALGERDCSIQRRHQKVVEETPSPALDDKRRQELLALAVKVAKAGRYTNAG
                     TAEFLMDQEGRFYFLEMNARLQVEHPVTEMVLGVDLVREQIKVAQGEKLGLKQEDVHP
                     EGASMEFRVYAEDPDQGFIPDAGTVRRLELPSGPGVRCDFGVRSGGVVPVYYDPMIGK
                     IVVWAPDRAGAIARGRRALRECVLEGIKTTLPFHRWLLEQEPFVQGDFDTGFLSRHFK
                     NADPVDQVQAEEDAAVLAAVFARIDAARPVLNAPSADGSGPADRRPMTTADATTTSRW
                     RDALQSHRARQGGGR"
     gene            3165..3665
                     /locus_tag="VFR25_05185"
     CDS             3165..3665
                     /locus_tag="VFR25_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9940475.1"
                     /translation="MSVVLSREGRDRAIDVTPSGDGFVVTVDGKEHRVEGAFGGLMRV
                     RIDDRPVEASVRRQGLDLVVDIRGRVYVYRPRDPRAPKLARKSVADQARGELHAPMPG
                     LVVDVMVAEGDTVDAGQAVVVVEAMKMQNALTAPLSGRVSAVRVTAGATVDSGALLLT
                     ILPEEG"
     gene            3667..5214
                     /locus_tag="VFR25_05190"
     CDS             3667..5214
                     /locus_tag="VFR25_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9940476.1"
                     /translation="MARSDLAKQGGQGGDPSPALGTPGEPPYLRGIHPSMYRSRVWTM
                     RQYAGFGTAEASNERYHYLLSLGQTGLSVAFDLPTQMGYDSDADRAQGEVGRVGVAIS
                     TIDDMERLLHDIPLDQVSLSMTINATAMILLALVVAVADRRGTPRAKLSGTVQNDVLK
                     EYIARGTYVYPPGPSLKIATDLFEFGTSEVPQWNTISVSGYHIREAGSTAVQEVAFTL
                     SDGLEYVRAAVDRGLPVDSFAPRVSFFFNAHNDLLEEIAKFRAARRLWARLMEERHHP
                     KDPNSLKLRFHAQTAGVTLTAQQPHNNIARVAIQALAAVLGGCQSLHTNSFDEAISLP
                     SEEAATTALRTQQILAWETGVPDTVDPVGGAPAIEERTDRIEREARAEIDAIDAMGGA
                     VRAIEAGYQARAIEKSAYETQLKMEKGEQIVVGVNRFGDPEDTARPIFTLDRRLEEQQ
                     RERVRAFKASRDQNLAKHALDSLELAARRELNLVEPTVDAVKAGATLGEISDVLRRVY
                     GTHDPRP"
     gene            5257..6117
                     /locus_tag="VFR25_05195"
     CDS             5257..6117
                     /locus_tag="VFR25_05195"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9940477.1"
                     /translation="MNLFQAAILGAVEGLTEFLPVSSTGHLILAAHAMGLVSEAMDAF
                     EIVIQAGALLAVLWLYRDRVASLFTGLAQPNSPGRALLLKLIVAFIPAAALGFVSHKW
                     IKAHLFGPRPVAIALIAGGLLILFVARWMRARDRTPGQPARFGLDDLPISAAVLIGLA
                     QCFSLWPGTSRAMTTILAALVLGATPVAAAEFSFLLALPTLGAATLFDLLKHHDELTG
                     PAIGGPGPLIVGLVVSAIVAAIAIRGFLRYVTSRGLEPFGWYRIVLGIVVIALLWHHG
                     AAAIQPPVAP"
     gene            6114..6527
                     /gene="mce"
                     /locus_tag="VFR25_05200"
     CDS             6114..6527
                     /gene="mce"
                     /locus_tag="VFR25_05200"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET9940478.1"
                     /translation="MSQAILGLAHVGIAVRELELAVTTWTKLGFELEGTELLESMHLR
                     LAFLKAGDSLIELLEPTSPESAVARFLERRGEGIHHLSFHVQDIDAALKHAAQAGFEL
                     IDRSARAGSHGTRIAFLHPRSLGGVLVEFCERRSP"
     gene            6524..7033
                     /locus_tag="VFR25_05205"
     CDS             6524..7033
                     /locus_tag="VFR25_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940479.1"
                     /translation="MRHLFRTPEFRVLAVVLGLVTLYATYACSKGGGRLAQPYPYMVG
                     DDVATRMSGIIRVVAGLEANTMVYYDRKDHRIVASVVGTASDVAGAERELDSFLQAVR
                     QFVFPYAKRAHGLDLSDSDVTLVYYNDGGEAGSPYEVVRRENGAYKPAPGGAPADSSS
                     DITNPGTTD"
     gene            7045..8493
                     /locus_tag="VFR25_05210"
     CDS             7045..8493
                     /locus_tag="VFR25_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940480.1"
                     /translation="MRRAALLTALALLSAGFTHGPLIPGPGSGVRTATPAMVRPLRED
                     VSVVPILTVGDTLAVSDTATVPYVFPPMPSGLGILPTSSGLAEIYVAHDLPWDPFVVG
                     GARVSRVAIDIRNLGALAGDFLIEGIDGYSRFASGTVGAARDGFLIPKFLVGEDRIDG
                     PGRGLFTAVDTRDGTVTNLPWLGRFAHGGSAIVPTSTGKMAVVLTEDDLPGESQLYMY
                     LADSDDALLHGRGQLFVFRADPGPERTRLSSRAARNAPITGSFVPIDTKFGNDFAPPD
                     RIEARAQSIGCMNFVRLRGATPDRSRANIFYFADAGAGNFYDPRTARLVTGAGRVYRA
                     ELDPIDPTKVIDLRVILDGDDGDDIYRPDGLASDSRYLWIQEDPGGVRSLHPARILRY
                     DMDARRLEVLAECVERDAKGALLSQGVGGVWKTAGIVDASEIFGTDTWLISVQAPNQR
                     QTRPWDQLGTGQILLLRGPGWTPPKAKKTKGS"
BASE COUNT         1342 a         2996 c         2914 g         1355 t
ORIGIN      
        1 cgcgattctg acggcgccgg gaaagggaca gtcgacccgc cgatggatac gctgaagtac
       61 ctcgaggatc tcgagcggcg cgcggagctc ggcggcggag aggagcggca gcgccgccag
      121 cacgaggccg gcaagctgac cgcgcgcgag cgcatcgagg tgctgctcga tcccggcagc
      181 ttcgtcgaga tggacaagtt cgtcacgcac cgctgcgccg acttcggcat ggagcagcag
      241 aagatcctcg gcgacggagt ggtcaccggc tacggcacgg tcgccggccg ccaggtgttc
      301 gtgttcgcgc aggacttcac ggtcttcggc ggcacgctct cggaagagaa cgcgaagaag
      361 atctgcaacg tgatggatct cgctttggaa aacggggcgc cgatcatcgg gttgaacgac
      421 tccggtggcg cgcgcatcca ggaaggcgtg cagtcgctgg gcggctacgc cgagatcttc
      481 ctgcgcaaca cgatcgcctc gggcgtggtg ccccagatct ccgcgatcat gggtccctgc
      541 gccggcggcg cggtctactc ccccgccatc accgacttcg tcctgatggt ccagggcacg
      601 agccacatgt tcgtgaccgg cccgaacgtg atcaagaccg tgaccaacga ggacgtctcg
      661 ttcgaagacc tgggcggcgc gatgagccac aacacgaaga gcggcgtggc tcacttcgcg
      721 gtcgaggacg acgaggcgtg cctcgcggcg atccgccggt tgttgagctt cctcccgcag
      781 aacaacgtcg acgatccgcc ccgcctggcg tcgaacgatc ctccggagcg catggacatg
      841 aagctcgcgg agatcgtccc ggccgagccg aacaagccgt acgacgtgaa ggacatcatc
      901 cggctcgtcg tggacgatgc ggatttcttc gaagttcacg agcactacgc gcggaacatc
      961 gtggtcggct tcgcgcgact cgacgggcgc tccattggcg tggtggcgaa ccagcctgcg
     1021 gcgctcgccg gcgtcctcga catcaactcg tcgacgaagg gagcgcgctt cgtccggttc
     1081 tgcgacgcgt tcaacattcc gctggtgacc tttgaagacg tgcccggctt cctccccggc
     1141 acgcagcagg agtggggcgg catcatccgg cacggcgcga agctcctgtt cgcgtactgc
     1201 gaagcgaccg tgcccaagct cacggtcgtg ctgcggaagg cctacggcgg cgcgtacgac
     1261 gtcatgtcgt cgaagcacat ccgcggcgac tacaacgtgg cctggcccgg cgcggagctc
     1321 gcggtgatgg gcgccgaggg agcggtgaac atcgtctacc ggaaggagat cgatggcgcc
     1381 aaggacccgg ccaaggcgcg ggcgaagctg atcgaggagt acaacgacaa gttcgcgaat
     1441 ccgtacgtcg ccgccgcgct cggctatctc gacgacgtca tcgagccgca ggagacgcgc
     1501 ccgcgcctga tccgcgcgct ccgcatgctc gcgcacaagc ggcagaccct gccgccgaag
     1561 aagcacggca acatcccgct ctaggagcga cccgccatgc cgaccaagcc gatccgcgcc
     1621 gtgctcgtat tgaatcgggg cgagatcgcg atccggatct gccgcacgct ccgcgagatg
     1681 ggcattcgct cgatcgccgt cttctccgac gccgatcgcg acgcgccgca cgtctttgcc
     1741 gcggacgcgg cctaccggct cggcccggcc gcggcctccc agagctacct caacggtgcg
     1801 aaggtgatcg aaaccgcgcg cgccgcaaga gccgatgcgg tccatcccgg ctatggcttc
     1861 ctcgcggagt cggccgactt cgcggagatg gtcgagaaag ccgggctcgt ctggatcggt
     1921 cccacacccg cggccacgcg cgccctcggc gacaaggtga gcgcgcgggc gatcgccatc
     1981 aacgcaggcg ttccggtgct ccccggcacc gaaggccccg tgagcgatcc ggcggaattg
     2041 aggcgcgcgg cggattcgat cggctacccc gtggtgctaa aggcggcggc aggcggcggc
     2101 ggcaagggca tgcgccgcgt gaacgcggcc ggcgagttcg agcaggcggt ccggctcacg
     2161 cagggcgagg ccaagggcgc gttcggcgac gaccgtctct acctcgagcg ctgggtcgag
     2221 cgtccgcgcc acatcgaggt gcagatcctg gccgaccgcc acggcaccgt gctggccctc
     2281 ggcgaacgcg actgctccat ccagcggcgg catcaaaagg tggtcgagga gacgccctcc
     2341 cccgcgctcg acgacaagcg gcgccaggag ctcctcgcac tcgcggtgaa ggtcgccaag
     2401 gcgggccgct acaccaatgc cggaaccgcc gagttcctca tggaccagga gggccgcttc
     2461 tacttcctcg agatgaacgc gcggctccag gtcgagcacc ccgtcacgga gatggtgctc
     2521 ggcgtggatc tcgtgcggga gcagatcaag gtcgcgcagg gagagaagct cgggctgaag
     2581 caggaagatg ttcatcccga aggcgcgtcg atggagttcc gggtctacgc cgaggatccg
     2641 gatcagggct tcatccccga tgcgggcacc gtgcgccggc tggagctgcc gtcgggcccc
     2701 ggcgtgcgct gtgatttcgg cgtgcgctcg gggggcgtcg ttccggtcta ctacgatccg
     2761 atgatcggaa agatcgtggt ctgggcgccg gatcgcgcgg gtgcgatcgc gcgcgggcgc
     2821 cgcgcgcttc gcgagtgcgt gctcgaggga atcaaaacca cgcttccctt ccatcgctgg
     2881 ctcctcgagc aggagccctt cgtgcagggt gatttcgaca cgggatttct gtcgcggcac
     2941 ttcaagaacg cggatcccgt ggaccaggtg caggcggagg aagatgccgc cgtgctcgcc
     3001 gcggtgttcg cacggatcga tgcggcccgg cccgtgttga acgctccgtc ggcggatggg
     3061 tccggccccg ccgatcgccg ccccatgacc acggcggacg cgaccacgac gagccggtgg
     3121 cgggacgcgc tccagagcca ccgcgcgcgc cagggaggcg gccgatgagc gtcgtcctca
     3181 gccgcgaagg gcgcgatcgc gccatcgacg tcacgccgag cggcgacggc ttcgtcgtga
     3241 ccgtggacgg gaaggagcac cgtgtcgagg gcgcgttcgg cgggctgatg cgcgtgcgga
     3301 tcgacgatcg ccccgtggaa gcgagcgtgc gccgccaggg gctcgacctc gtcgtcgaca
     3361 tccgcggccg cgtctacgtc tatcgcccgc gcgatccgcg cgcccccaag ctcgcgcgca
     3421 agagtgtcgc ggaccaggcg cgcggtgagc tgcacgcgcc catgcccggc ctcgtcgtcg
     3481 atgtcatggt tgccgagggc gacaccgtgg acgcgggaca ggccgtcgtg gtggtcgagg
     3541 cgatgaagat gcagaacgcg ctcacggctc cgctctccgg ccgcgtgtca gccgtgcgcg
     3601 tcaccgcggg cgccacggtc gattcgggcg cgctccttct gacgatcttg cccgaggagg
     3661 gctgatttgg cccggtcgga cctcgcgaag caaggcggcc agggcggcga ccccagccct
     3721 gccttaggca cacccggcga gcctccctac ctccgcggca tccatccttc gatgtaccgc
     3781 tcgcgagtct ggacgatgcg ccagtacgcg ggcttcggga ccgccgaggc atcgaacgag
     3841 cgctaccact acctcctctc gctgggccag acgggcctct ccgtggcatt cgacctcccc
     3901 acccagatgg gttacgactc cgacgccgac cgcgcgcagg gcgaggtggg acgcgtgggg
     3961 gtcgcgatct cgacgatcga cgacatggag cggctcctcc acgacatccc gctcgaccag
     4021 gtctcgctct ccatgacgat caacgcgacc gcgatgatcc ttctcgctct cgtggtcgcg
     4081 gtcgcggacc ggcgcggcac cccgcgcgcc aagctctccg gcaccgtgca gaacgacgtg
     4141 ctgaaggagt acatcgcgcg cggcacctat gtttatccgc ccggcccctc gctcaagatc
     4201 gcgaccgacc tgttcgagtt cggcacgtcc gaggtgccgc agtggaatac gatctcggtg
     4261 agtggctacc acattcgcga ggcgggctcg accgcggtgc aggaggtcgc gttcactctg
     4321 tcggacgggc tcgagtacgt gcgtgcggcg gtggatcgcg ggctccccgt cgacagcttc
     4381 gccccccggg tctccttctt cttcaacgcg cacaacgatc ttctcgaaga gatcgccaag
     4441 ttccgcgcgg cgcggcgtct ctgggcccgg ctcatggagg agcgccacca tcccaaggat
     4501 cccaactcgc tcaagctccg cttccacgcg cagacggcgg gcgtgacgct caccgcgcag
     4561 cagccgcaca acaacatcgc gcgcgtcgcc atccaggcgc tggcggcggt gctcggcggg
     4621 tgccagtcgc tgcacacgaa ctccttcgac gaggcgatct cccttcccag cgaggaggcc
     4681 gcgaccacgg cgcttcggac gcagcagatc ctcgcgtggg agacgggtgt ccctgatact
     4741 gtggacccgg tcggcggcgc gcccgccatc gaggagcgga ccgaccggat cgagcgggag
     4801 gcgcgcgcgg agatcgatgc gatcgacgcc atgggcggcg cggtccgggc gatcgaggcg
     4861 gggtaccagg cgcgcgcaat cgagaagagc gcctacgaaa cacagttgaa gatggagaag
     4921 ggagagcaga ttgtggtggg cgtgaatcgc ttcggagatc cggaggacac cgcacggccg
     4981 atcttcacgc tcgaccgccg gctcgaggag cagcagcgcg agcgtgtgcg cgcgttcaag
     5041 gcgtcgcgcg accagaatct cgcgaagcac gctctggatt ccctggagct agcggcgcgg
     5101 cgggagctga acctcgtcga gccgaccgtc gacgcggtca aggcgggcgc cacgctcggc
     5161 gagatcagtg acgtgctccg gcgcgtctac gggacccatg atcctcgccc ctaggccagc
     5221 gggccgcacc cgccctggcc ccgcgggccg caccgcgtga atcttttcca ggccgccatt
     5281 ctcggcgcgg tcgaggggct cacggagttc ctccccgtct cctccaccgg ccatctcatc
     5341 ctcgccgccc acgcgatggg cctcgtcagc gaggcgatgg acgcattcga gatcgtcatc
     5401 caggcgggag cgctgctcgc ggtgctgtgg ctctaccgcg atcgcgtcgc gagcctgttc
     5461 accggcttgg cgcagcccaa ttcgccggga cgcgcgctcc tcctcaagct catcgtcgcc
     5521 ttcatccccg cggccgcgct tggcttcgtg agccacaagt ggatcaaggc gcacctcttc
     5581 ggaccgcgcc ccgtggcgat cgcgctcatc gcgggcgggc ttctcatcct cttcgtcgcg
     5641 cgctggatgc gcgcgcggga tcgaacgccg ggccagccgg cccgcttcgg cctcgacgat
     5701 ctgccgatct ccgcggcggt cctgatcggg ctcgcgcagt gcttctcgct ctggcccggt
     5761 acgtcgcgcg ccatgaccac gatcctcgcc gcgctcgtgc tcggcgccac gcccgtcgcc
     5821 gcggcggagt tttcgttcct tctcgcgctc ccgacgctcg gcgcagccac cctgttcgat
     5881 ctcctcaagc accacgacga gctgaccggc cccgcgatcg gcggccctgg gccgctcatc
     5941 gtgggcctcg tggtctcggc gatcgtggcc gccatcgcga tccgagggtt cctgcgctac
     6001 gtcacgagcc gcggcctcga gcccttcggc tggtaccgga tcgtgctggg catcgtcgtg
     6061 atcgcgctgc tctggcacca cggcgcggcg gcgattcagc cgcccgtggc gccgtgagcc
     6121 aggccatcct cggcctcgcg catgtcggga tcgccgtgcg cgagctcgag ctggcggtca
     6181 cgacctggac caagctcggc ttcgagctcg aaggcaccga gctgctcgaa tccatgcacc
     6241 tccgcctcgc cttcctcaag gccggcgact cgctgatcga gctcctggag cccacgtctc
     6301 ccgaatccgc cgtcgcgcgc ttcctcgagc ggcgtggaga aggcatccat cacctctcgt
     6361 tccacgtcca agacatcgac gcggcgctga agcacgccgc ccaggccggt ttcgagctga
     6421 tcgatcggag cgcgcgcgcc ggaagccacg gcacgcgcat cgcgtttctc cacccccggt
     6481 cgctcggggg cgttctggtg gaattctgtg aacggaggag cccatgaggc acctgtttcg
     6541 gacgcctgag ttccgcgttc tcgccgtcgt tctcggcctg gtcacgctgt acgcgaccta
     6601 cgcgtgctcc aaggggggcg gacgcctcgc ccagccctac ccctacatgg tcggggacga
     6661 cgtcgcgacg aggatgtcgg ggatcatccg cgtggtggcg ggactcgaag cgaacacgat
     6721 ggtgtactac gaccggaagg accatcggat cgtggcttcg gtcgtgggga ccgcgtccga
     6781 cgtggcggga gccgagcggg agctggacag cttcctccag gccgtgcgcc agttcgtgtt
     6841 tccgtacgcc aagcgtgcgc acgggctcga cctctccgac agcgatgtga cgctcgtcta
     6901 ctacaatgac ggcggcgagg caggctctcc ctatgaagtg gtccgtcgcg agaacggcgc
     6961 gtacaagccg gcaccgggcg gcgctcccgc ggactcgtcg tcggacatca cgaaccccgg
     7021 aaccaccgat taggagtctt ctccatgcgt agagcagccc tgctgaccgc cctggcgctc
     7081 ctgagcgccg gcttcacgca tgggccgctc atcccgggtc ccggttcggg cgtgcgcacg
     7141 gccacgccgg ccatggtgcg gccgctccgt gaagacgttt cggtcgtccc catcctgacg
     7201 gtcggcgaca cgctcgcggt atcggacacc gcgaccgtcc cctatgtctt cccgccgatg
     7261 ccctccgggc tcggcatcct gccgaccagt tccgggcttg ccgagatcta cgtcgcgcac
     7321 gacctgccgt gggatccctt cgtcgtcggc ggcgcgcgcg tctcgcgcgt cgcgatcgac
     7381 atccggaacc tgggcgccct cgcgggcgat ttcctcattg agggaatcga cggctactcg
     7441 cgcttcgcct cgggaaccgt cggcgccgca cgcgacggat ttctcattcc caaattcctc
     7501 gtgggcgagg accggatcga cggaccggga cgcgggctct tcaccgccgt ggacacgcgc
     7561 gatggaaccg tgacgaatct cccctggctc ggccgcttcg cccacggcgg atcggccatc
     7621 gttcccacgt cgacgggaaa gatggcggtc gtgctcaccg aggacgacct gccgggcgag
     7681 tcgcagcttt atatgtatct cgccgatagc gacgatgccc tgctccacgg gcgcgggcag
     7741 ctcttcgtgt tccgcgccga tcccggcccc gagcgaacgc ggctctcctc gcgcgccgca
     7801 cggaacgcgc cgatcaccgg cagtttcgtg ccgatcgaca cgaagttcgg gaatgacttc
     7861 gcaccacccg accggatcga ggctcgcgcg caatcgattg ggtgcatgaa cttcgtgcgg
     7921 ctccgtggcg ccacgccgga ccgctcgcgc gccaacatct tctacttcgc cgatgccggc
     7981 gcgggcaact tctacgatcc gcggaccgct cgcctcgtaa ccggcgcagg ccgcgtgtac
     8041 cgcgcggagc tcgaccccat cgatcccacg aaggtgatcg accttcgcgt gatcctggac
     8101 ggggacgacg gcgacgacat ctaccgcccc gacggactcg cctcggactc gcgctacctc
     8161 tggatccagg aggatccggg cggcgtgcgg agcctccatc ccgcgcgcat tctccgctac
     8221 gacatggacg cgaggcggct cgaggttctg gctgagtgcg tggagcggga cgcgaagggt
     8281 gcgcttttgt cgcagggcgt gggcggagtg tggaagacgg cgggaatcgt ggacgcgagc
     8341 gagatttttg gcacggacac gtggctcatc tcggtccagg cgccgaatca gaggcaaacc
     8401 cgcccatggg accagcttgg cacagggcag atcctgctcc tgcgcgggcc ggggtggact
     8461 cctcccaagg cgaagaagac caagggaagc tgatctagac cgggtagacg gggcacagtg
     8521 ggcccggtgg gccgccaact ccgtttccgc acctagctgc cgtcggcacg gacacttacg
     8581 ccagaaaccc tgcatgggta ttttcct
//
LOCUS       DASTKC010000126         8967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1748450, whole genome shotgun sequence.
ACCESSION   DASTKC010000126 DASTKC010000000
VERSION     DASTKC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748450"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /gene="gpmI"
                     /locus_tag="VFR25_05215"
     CDS             <1..1181
                     /gene="gpmI"
                     /locus_tag="VFR25_05215"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9940481.1"
                     /translation="LLSPGGVHSHERHLAAMIRHAIARGVARIRVQAFLDGRDTAPRS
                     AGASIRGLEAVLRDVKDGKIATISGRYYAMDRDKRWDRTEKAYRAMANGDGRTAGSAE
                     AALDEAYARGESDEFVDPTVVRGADGAIRNGDTVVAFNFRPDRMRQISRALADPGFDA
                     FPRPGGAPPIHYTCMTEYDETFRFPVIFTDEPLKGTIGEVVSRAGIRQLRIAETEKYA
                     HVTYFFNGSEETPFQGEDRALIPSPKVATYDLKPEMSAREVTDEVVRRLTGEPYGFFV
                     LNYANADMVGHTGVLEAAIRAVETVDGCLGRVLQAVESRGGIALVTADHGNAEQMIDE
                     ATGGPHTAHTLNPVPILAAGGPHITLQSGILADVAPTLIELLGLDPSPEMTGTSLIRP
                     "
     gene            1719..2612
                     /gene="sppA"
                     /locus_tag="VFR25_05220"
     CDS             1719..2612
                     /gene="sppA"
                     /locus_tag="VFR25_05220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9940482.1"
                     /translation="MARNRWLLGCALLLVGTVATVALAFLVVNMALSGDRPAWGGLGG
                     RVGLVEILGEIDDSDDIVDQIERLDRDASVKAVVVRLDSPGGGVAASQEIYEALRKLH
                     DDKKPVVASFAGVAASGAYYVACAADSIVSNPGTLTGSIGVIMSFPNTEELFRKVGLH
                     FEVIKTGKYKDVGSMWRPMTAEEHELLQNVLSNVYDQFVDAIVDGRGMEREDILPYAD
                     GRVFSGDQALEYGFVDRLGDLDDAIDMAAKLGGIRGEPTIVRKERHKVGLLDLLDEKM
                     NQVGEAAGFSSKGPRLEYRLR"
     gene            2691..2969
                     /locus_tag="VFR25_05225"
     CDS             2691..2969
                     /locus_tag="VFR25_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9940483.1"
                     /translation="MTKADLVDEISSSTKISKSETATIVDQLLNAISRALSEGKHIEI
                     RGFGTFKVRERKARKARNPRSGAEVMVPAKLVPVFKASNELKGAVRGG"
     gene            3225..5204
                     /locus_tag="VFR25_05230"
     CDS             3225..5204
                     /locus_tag="VFR25_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HET9940484.1"
                     /translation="MTQRTQVGRLERTRFGYGFVRVEDGGEDLFIPPFAMGGAFHGDR
                     VRAGYLDSGPQGDAHEVLEVLERTKYGILGRFEGRGRNALLLPERPEYPREILLTLHG
                     RARIPPRTRVLVRLNPTPPEPVVGTVEAVFADDDPREDSWIVALEEGIRTEFDPEAER
                     EAAAFREDSVAQAIRGRTDFRASRVLTIDPADAKDHDDALSIEAVNGGVTTVGIHIAD
                     VTHYVRPGTALDKEARERGTSVYLADTTYPMLPEALSSTLCSLTEGDDRLTVSVLADI
                     AENGALVGARVVEGVIRSAASLSYPEAHRILREDSGDTAVALRTLDRLANALRERRFE
                     KGGLELDLPEIKPELDAQGDPVRFDETPRLPTHGLIEEFMLLANQIVGRRAHERELPF
                     LYRVHERPRYDKLRSFFEAARYLGKQGPAQIVTDARQLRRWISHGRAPKDRLLNLYLL
                     RALEKARYDLVDVGHFGLGMAGYAHFTSPIRRYPDLANHRIVKRFLLEEHARAGGDPW
                     AFAGEWLNASVAGRATEAEIAADDAERDVEKRKAVRFALQRVGEEARGIIAGLTPGGL
                     FVWIPEWSIEGFLPKRSLGDPSLSLAEHGFSFRSKRSRHRFGLGDAVTVVVSRADLDR
                     REVELGLLPPAGGGPRQKRRAQGKRAAHRRPKGRR"
     gene            5245..5679
                     /locus_tag="VFR25_05235"
     CDS             5245..5679
                     /locus_tag="VFR25_05235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9940485.1"
                     /translation="MTKPTRERRQSPRISAKLAMQVAGTDEASVVTTESINLSASGIQ
                     FGSRHFLSPLTKVALTLLLPPFGRSLRRERMLQVQGVIVRCEERTGPRQQTRYELACY
                     FTDLAEGDRELIEQYILWRTVRRIPTTNGEPEPARPRRSAAP"
     gene            5771..6799
                     /locus_tag="VFR25_05240"
     CDS             5771..6799
                     /locus_tag="VFR25_05240"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET9940486.1"
                     /translation="MTFSSLRTRLPALLALSAGLLLLAVPAHATRYAGEFLRVGVGAR
                     ALGMGSAFAGLADDGTAAYWNPAGLATLERHELQAMHAEQFGSIVQYDYLSYSAPLGE
                     PGKAKQGLALSLIRLGVDEIPDTRGLEIIDVNGNGRFDYPEDRLVVDESRFVFDSDND
                     VALLFSYAREVSRGFSLGGNVKLVHQWLGDSLRSNGFGLDVGALYLGPHGWSAGAKLS
                     DATTTRILWNTGTDEFIAPSLRLGFAKTTAFSNRKHIITAAFDVDMGFSDEQLSSQAN
                     LGSVTFLFHPGLEYWLERKLALRTGFEGKNFAAGTGFRFKKFGLDYAYLDHQDLDSSH
                     RISASYAF"
     gene            6854..8101
                     /locus_tag="VFR25_05245"
     CDS             6854..8101
                     /locus_tag="VFR25_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase family
                     protein"
                     /protein_id="HET9940487.1"
                     /translation="MLAGGKSERLHALGRMRTASALPFGGKYRIIDFTLSNCVHSGLY
                     RIGVLTQYAPLSLQEHIGIGRAWDLDRRDGGIRLLPPYVRQRATNWYRGTADALVQNR
                     NVIADAQARHILVVSGDLIYKMDYGSLIARHEARGSVLTVVTTRSPDDEAERYGIVDV
                     DGAGRVRGVEVRRDGGPGTQVSASIYLFRGRDLLARLADPSLGPDLVADVIRPMIEEG
                     LPVHADLFDGYWHDVTTIDSYYAASMDLLRPLPPINLNDPDWLVYTPSEDRAPSLLGG
                     TAEVSSSMIAHGTRIEGTVRRSILFPGVHVGAGAVVEDSIVMHDTRILPGARLHGAII
                     DKQVRIGERAEVGVGEPVRHREHPHDLGSGLCVIGKGAEVPAGAKVGRNTLVEIGVRE
                     RDYGRDEVPSGSTVRPRPVGAAR"
     gene            8098..>8967
                     /locus_tag="VFR25_05250"
     CDS             8098..>8967
                     /locus_tag="VFR25_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9940488.1"
                     /translation="MISGVQYAMILAGGVGSRLCLLSEKRAKPAVPFGGKYRIIDFTL
                     SNCVNSGIFDVGILTQYRPLSLRDHIGIGRAWDLDRKRGGVELLQPFQGWAENDWYRG
                     TADAVWQNWHRVADRQAEHILVLSGDHVYRMDYRPMIEAHLERGADATVAITEIPIEQ
                     ASQFGVVRTDASGWITGFLEKPKNPPSGRISMGVYVFRVAALLQELVKDDERNSAHDF
                     GRDILPALVERGRVAGFLVPGYWQDIGTLDSFYQANLDLLSEDPPFPLEDARWPIFTP
                     STEYPPARIGPGAH"
BASE COUNT         1458 a         3076 c         3047 g         1386 t
ORIGIN      
        1 ggctcctctc gcccggcggc gtccacagcc acgagcggca cctcgccgcg atgatccgtc
       61 atgcgatcgc gcgcggcgtg gcgcggatcc gcgtgcaggc gttcctggac ggacgcgaca
      121 cggctccgcg ctcggccggc gcgtcgatcc gcggactcga ggccgtgctc cgcgacgtca
      181 aagacgggaa gatcgcgacg atctccgggc gctactacgc gatggatcgc gacaagcgct
      241 gggatcgcac cgagaaggcg taccgagcga tggcgaatgg ggacgggcgc acggcaggct
      301 ccgcggaagc cgcgctcgac gaggcgtacg cgcgcggaga atcggacgag ttcgtggatc
      361 ccacggtcgt gcgcggcgcg gacggagcga ttcggaacgg cgacacggtc gtggccttca
      421 acttccgccc cgaccgcatg cggcagatct cgcgcgccct cgccgatccc gggttcgacg
      481 catttccacg cccgggcggc gccccgccga tccactacac ctgcatgacg gagtacgacg
      541 agacgttccg cttcccggtg atcttcaccg acgagccgct gaagggcacg atcggcgagg
      601 tcgtgagccg cgccgggatc cgccagctca ggatcgccga gacggagaag tacgcccacg
      661 tcacgtattt cttcaacggc agcgaggaga ctccgttcca aggcgaggac cgcgcgctca
      721 ttccctcgcc caaggtggcc acctacgatc tgaagccgga gatgagcgcc cgcgaggtga
      781 ccgatgaagt ggtgcgtcgc ctcaccgggg agccctacgg cttctttgtg ctcaactacg
      841 cgaacgcgga tatggtgggg cacacgggcg ttctggaggc cgcgatccgc gcggtcgaga
      901 ccgtggacgg ctgcctcggc cgtgtcctcc aggcggtcga gtcgcgcgga ggaatcgccc
      961 ttgtcaccgc cgaccacggg aatgccgagc agatgatcga cgaagcgacc ggaggccctc
     1021 acacggccca caccctcaat ccggtcccga tcctggcggc cggcggcccc cacatcaccc
     1081 tccagtccgg catcctggcg gacgtcgctc ccacgttgat cgagctcctg ggactcgacc
     1141 catccccgga gatgaccggg acctcgctca tccgtccgta acttctgggg gttttcacgc
     1201 ccgcctttgg gggcgtcgac cccgatccag ccaccgcgtg gcccgttcga agcctcttcg
     1261 acacgcgcgg ccaaggcgcg ccgcagacga ttcgcgctgc actgcaatcc acccacttaa
     1321 ctaattgtaa aatcacatgt taacggtggc ctgttcgagc catcgccgcg aaggacgcga
     1381 ctcgattttc cgcgggcgcg ccgcgtcgtg gtccagcggt tgctgtaggt acatggcgta
     1441 gggcgcagcg aaaaaactga aaaccaagtc ttgttccctg cggtgctcgc gagaatcgtc
     1501 cggtcctgag ctgatcccct cgacgagacg acgcagttcc aacggcaccg gatgcacggc
     1561 acacgtgggg aacatgtaat ccaagggccc gactcccccc gggtccaccc aaggcgccgc
     1621 ggcgagaccg cggcgccttc ctcctttcac agcccgcctc cggtctgcta cgttcattcc
     1681 gtctatcctc agtgctgaac cagggatcgg gagggcgttt ggctcggaat cgctggctcc
     1741 taggctgtgc gctcctgctg gtcgggacgg tggcgaccgt ggctctcgcg ttcctggtgg
     1801 tcaacatggc cctgagcgga gaccgccccg cctggggagg attgggcgga cgcgtcggcc
     1861 tcgtggaaat tctcggcgag atcgacgatt cggatgacat cgtggaccag atcgagcggc
     1921 tcgaccgtga cgccagcgtg aaggcggtcg tggtccgcct cgacagtccg ggcggcggcg
     1981 tggcggcgtc tcaagagatc tacgaggcgc tccggaagct gcacgacgac aagaagcccg
     2041 tggtcgcgtc cttcgcgggc gtggcggcgt cgggcgcgta ctacgtcgcg tgcgcggccg
     2101 attccatcgt ctcgaatcca gggacgctca cgggctcgat cggcgtgatc atgagctttc
     2161 cgaacaccga ggagctgttc cggaaggtgg gccttcactt cgaggtgatc aagacgggga
     2221 aatacaagga cgtcgggagc atgtggcgcc cgatgacggc cgaggagcac gagcttcttc
     2281 aaaacgtgct gtccaacgtg tacgaccagt tcgtggatgc gatcgtcgac ggccgcggca
     2341 tggagcggga ggacatcctg ccctacgccg acggacgggt gttctcggga gaccaggccc
     2401 tcgagtacgg attcgtggac cggctcgggg atctcgacga cgcgatcgac atggccgcca
     2461 agctgggagg gatccgggga gagcccacga tcgtccgtaa ggagcgtcac aaggttggtc
     2521 ttttggatct gctcgacgag aagatgaacc aggtcgggga agccgccggg ttctcttcga
     2581 agggaccccg tctggagtat cgcctgcgtt gattgacggc cccgtggccc aatgctagat
     2641 tctggcctgg ctacggcggt tacgcggccc gagccgatgg agggatgaac atgacgaaag
     2701 ccgatctcgt ggacgagata tcgtcctcga ccaagatttc gaagagcgag acggccacga
     2761 tcgtcgatca gctgttgaac gcgatctcgc gagcgttgag cgaagggaag catatcgaga
     2821 tccggggatt cgggaccttc aaggtgcggg agcgcaaagc ccgcaaggcc cggaacccga
     2881 gatcgggcgc cgaggtgatg gtgccggcga agctggtgcc ggtcttcaag gcgtcgaacg
     2941 agctgaaggg cgcggtccgc ggcggttgac ctgcgggcgg ccgaaggaga ccgatgggaa
     3001 gcggaaagaa aaggaagcgg aagaagatcg ccactcacaa gcgcaagaag cggctccggc
     3061 gcgatcggca caagaagaag cttcgctgat cgagcggtgc gcgcggctct tctccgccgc
     3121 tcccggaccg gtccccggcc cgacggcccc tgttggggga cgttcgggcc ggccctcttt
     3181 accccatgac tccgcgccgc atccgtcgtg atcccacccc gccgatgacc cagcgcactc
     3241 aggtggggcg cctcgagcgc acgcgcttcg gctacggatt cgtccgtgtc gaggatggcg
     3301 gcgaggacct cttcatcccg cccttcgcca tggggggcgc gtttcacggg gatcgtgtgc
     3361 gggccggcta cctcgactcg ggcccgcaag gcgacgcgca cgaagtgctc gaggtcctcg
     3421 agcgaacgaa gtacggcatc ctcggacgct tcgaagggcg cggacggaac gcgctcctcc
     3481 tcccggagcg gcccgaatat ccccgcgaga tcctgctcac gctccatggc cgcgcgcgca
     3541 tcccgccgcg cacgcgcgtc ctcgtgcgtc tgaacccgac gccccccgag ccggtcgtcg
     3601 gcacggtcga ggccgtgttc gcggacgacg acccacgcga ggacagctgg atcgtcgcgc
     3661 tggaggaggg gatccggacc gagttcgatc ccgaagccga gcgcgaggcg gccgcgttcc
     3721 gcgaggactc ggtggcgcag gcgattcgtg ggcgcaccga cttccgtgcg agccgcgtgc
     3781 tcacgatcga tccggccgac gcgaaggacc acgatgacgc gctctcgatc gaggccgtga
     3841 acggaggcgt gaccacggtc ggcatccaca tcgccgacgt gacgcactac gtccggccgg
     3901 gcaccgccct cgacaaggag gcgcgcgagc gcgggacgag cgtctatctt gcggacacga
     3961 cctatccgat gctgcccgag gcgctctcgt ccacgctctg cagcctgacg gaaggggacg
     4021 accggctcac ggtgtccgtg ctcgcggaca tcgcggagaa cggcgcgctg gtcggcgcgc
     4081 gcgtcgtcga gggcgtgatc cggagcgcgg cctcgctcag ctatcccgag gcgcaccgga
     4141 tcctgcgcga ggattcggga gacaccgcgg tcgccctccg cacgctcgat cggctcgcga
     4201 acgcgcttcg cgagcggcgc ttcgagaagg gcggcctcga gctcgacctg ccggagatca
     4261 agccggagct ggacgcgcag ggagatccgg tgcgcttcga cgagacgccg cggctgccga
     4321 cgcacgggct gatcgaggag ttcatgctgc tcgcgaacca gatcgtggga cggcgcgctc
     4381 acgagcgcga gctccccttt ctttaccggg ttcacgagcg accccggtat gataagctgc
     4441 gctccttctt cgaggcggca cgttatctcg gcaaacaggg accggcgcag atcgtcacgg
     4501 atgcgcggca gcttcggcgc tggatcagtc acggccgcgc gccgaaagat cggctcctca
     4561 acctgtacct gctgcgtgcc ctggaaaaag ctcgctacga tctggtcgac gtgggtcatt
     4621 tcggactggg aatggcggga tacgcgcact tcacgtcccc catccgccgg tatcccgacc
     4681 ttgcgaatca ccggatcgtg aagcggttcc tgctcgaaga acacgcgcgc gccgggggcg
     4741 atccctgggc gttcgcgggg gagtggctca acgcgtcggt cgcgggacgc gccacggagg
     4801 cggagatcgc cgccgacgac gcggagcgcg acgtcgagaa gaggaaggcg gtgcgtttcg
     4861 cgctccagcg cgtgggtgaa gaagcgcggg gcatcatcgc gggactcacc ccgggcgggc
     4921 tcttcgtctg gattcccgaa tggagtatcg agggattcct gccgaagcgg tcgctcggcg
     4981 atccgtcgct gagcctcgcg gagcacggtt tttcgttccg gtccaagcgg tcccgtcacc
     5041 gcttcggcct gggcgacgcg gtcacggtcg tggtcagccg cgccgacctc gaccggcggg
     5101 aagtcgaatt ggggcttctc cccccggcgg ggggcggccc gagacagaaa cggcgggcgc
     5161 aagggaagcg cgccgcccac cgtcgcccca agggccgccg ttagcggtgc cctccggaac
     5221 gacgccccca caaggagaga cctcatgacg aagccgaccc gcgagcgccg ccaatcgccg
     5281 cgcatctccg ccaagctcgc gatgcaggtc gcaggaacgg acgaagcgtc ggtcgtcacc
     5341 accgagtcca tcaacctctc cgcgagcgga atccagttcg ggagccgcca cttcctgtcg
     5401 ccgctcacga aggtggcgct caccctgctc ctgccgccct tcggacggag cctccggcgc
     5461 gagcggatgc tccaggtcca gggggtgatc gtccggtgcg aggagcggac cggaccccgc
     5521 cagcagaccc gctacgagct ggcctgctac ttcaccgacc tggccgaggg ggatcgggag
     5581 ctgatcgagc agtacatcct ctggcgcacc gtccgccgca tcccaaccac gaacggcgag
     5641 ccggagccgg cgaggccccg tcggagcgct gcgccgtgat ccgggccgcc ggggggaacc
     5701 ggcggcttcg cccgcgggta gttgcccagg agatgacgcc ttcccaggca aggggtccca
     5761 tcatccggcc atgacctttt cgagtctccg cacgcgcctt ccggccctcc tggccctgtc
     5821 ggccgggctc ctcctgctcg ccgtccccgc ccacgcgacc cgctacgccg gggaattcct
     5881 ccgcgtcggg gtcggtgcgc gcgcgctcgg catgggcagc gccttcgccg gcctcgccga
     5941 cgacgggacg gccgcgtact ggaatccggc ggggctcgcc acgctggagc ggcatgaact
     6001 ccaggccatg cacgcggagc agttcggatc catcgtgcag tacgactacc tgtcctactc
     6061 cgcgcccctg ggtgagccgg ggaaggcgaa gcaagggctc gcgctctcgc tcatccgcct
     6121 cggcgtggac gagatccccg acacgcgcgg gctcgagatc atcgacgtga acggaaacgg
     6181 acgcttcgat tatcccgagg accgcctcgt cgtggacgag agccgcttcg tgttcgacag
     6241 cgacaacgac gtcgcgctcc tcttcagcta cgcgcgcgaa gtgagccgcg ggttctcgct
     6301 cggcggcaac gtgaagctcg tgcaccagtg gctgggggac agtctccgct cgaacggatt
     6361 cggcctcgac gtcggagcgc tctacctggg cccgcacggc tggtcggcgg gcgcgaagct
     6421 gagcgacgcc accaccacac ggatcctctg gaacaccggc acggacgagt tcatcgctcc
     6481 ctccctgcgc ctcggcttcg cgaagacgac ggccttctcg aaccggaagc acatcatcac
     6541 cgcggcgttc gacgtggaca tgggcttctc cgacgaacag ctctcgagcc aggccaacct
     6601 cggctcggtg acgttcctgt tccatcccgg cctcgaatac tggctcgagc gaaagctcgc
     6661 cctccggacc ggcttcgagg ggaagaactt cgcggcagga accggcttcc gcttcaagaa
     6721 attcgggctc gactacgcgt acctggacca tcaggatctg gattcgagcc accgcatctc
     6781 cgcttcctac gctttctagg cgctccgcgc cggaggtccg cgcctgaccg tcgtcccgcc
     6841 gatcctcgtc ctcgtgctcg cgggggggaa gagcgagcgg ctccacgcgc tcgggcgcat
     6901 gcgcacggct tcggcgctcc ccttcggcgg caagtaccgc atcatcgact tcaccctctc
     6961 gaactgcgtc cactcgggcc tttaccggat cggcgtgctc acgcagtacg cgccgctgtc
     7021 gctccaggag cacatcggga tcggccgggc gtgggacctc gaccggcggg acggcggcat
     7081 ccgccttctt ccgccctacg tgcggcagcg cgcgacgaac tggtaccgcg gcaccgccga
     7141 cgcgctcgtc cagaaccgga acgtgatcgc ggacgcgcag gcgcggcaca tcctcgtcgt
     7201 ctcgggggac ctcatctaca agatggacta cggatcgctg atcgcgcgcc acgaggcccg
     7261 cggctcggtc ttgactgtgg tcacgacgcg gagccccgat gacgaggcgg agcgctacgg
     7321 catcgtcgac gtggacggcg cgggccgggt ccgcggcgtc gaggtgcgcc gcgacggagg
     7381 gccggggact caggtgtcgg cctcgatcta tctcttccgc ggccgcgatc tgctcgcgcg
     7441 gctcgccgat ccttcgctcg gccccgacct cgtggcggac gtcatccgtc cgatgatcga
     7501 ggagggtctc ccggtgcacg cggatctctt cgacggctac tggcacgacg tgacgacgat
     7561 cgattcgtac tacgcggcga gcatggatct cctgcgcccg cttcccccca tcaacctcaa
     7621 cgatcccgac tggctcgtct acacgccgag cgaggaccgc gcgccgtcgc tcctgggggg
     7681 aaccgccgag gtctcgtcga gcatgatcgc gcacggcact cgcatcgaag ggaccgtgcg
     7741 ccgctcgatc ctcttcccgg gcgtccacgt gggtgcgggg gcggtcgtcg aggactcgat
     7801 cgtgatgcac gacacgcgca tccttccggg cgcgcgactc cacggcgcca tcatcgacaa
     7861 gcaggtgcgg atcggcgagc gggcagaggt cggcgtcggc gagcccgtcc gccaccgcga
     7921 gcacccgcac gatctgggga gcggtctctg cgtgatcgga aagggggccg aggtgccggc
     7981 cggcgccaag gtcgggcgga acacgctcgt cgagatcggc gtgcgcgagc gcgactacgg
     8041 gcgcgacgag gtgccctccg gctcgacggt gaggccgcgg cccgtggggg ccgcgcgatg
     8101 atcagcggcg tccagtacgc gatgatcctc gcgggcggcg tcgggagccg gctctgcctc
     8161 ctctcggaga agcgcgccaa gcccgccgtt cccttcggcg gcaagtaccg catcatcgac
     8221 ttcacgctct ccaactgcgt gaactcgggc atcttcgacg tcggcatcct cacccagtac
     8281 cggccgctct cgctgcgcga ccacatcggg atcggccgcg cctgggatct cgatcgaaag
     8341 cgcggcgggg tcgagctcct ccagcccttc caggggtggg cggagaacga ctggtaccgc
     8401 ggcaccgcgg acgcggtctg gcagaactgg catcgcgtcg cggatcgcca ggcggagcac
     8461 atcctggtgc tctcgggcga ccacgtgtat cgcatggact accgccccat gatcgaggcg
     8521 catctggagc ggggcgccga cgcgacggtc gcgatcaccg agattccgat cgagcaggcg
     8581 tcccagttcg gcgtggtgcg caccgacgcc tcgggatgga tcacgggctt cctcgagaag
     8641 ccgaagaatc cgccctcggg gaggatctcg atgggcgtgt acgtgttccg cgtcgcggcg
     8701 ctcctgcaag agctggtgaa ggacgacgag cggaactcgg cgcacgactt cggccgggac
     8761 atcctccccg cgctcgtgga gcgcggacgc gtcgccggct ttctcgtccc cggctactgg
     8821 caggacatcg gcacgctcga ctccttctac caggccaacc tcgatctcct gagcgaggac
     8881 cctccgtttc cgctcgagga tgcccgctgg cccatcttca cgccctccac cgagtatccg
     8941 ccggcacgca tcggacccgg cgcgcac
//
LOCUS       DASTKC010000127         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_330781, whole genome shotgun sequence.
ACCESSION   DASTKC010000127 DASTKC010000000
VERSION     DASTKC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330781"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFR25_05255"
     CDS             <1..756
                     /locus_tag="VFR25_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HET9940489.1"
                     /translation="LATLPVDRSAAPVPGARGGTAAGLRALRLFLKGPLQRYHLDRNR
                     PPIDGTSRLSAYLHFGHLGPQTVALAVRSAPAPRAAKQAYLEELIVRRELAVNFVARN
                     AQYDDVASAPDWGRRTLERHARDPREEIYSWDALEAGATKDRLWNAAQREMVATGRMH
                     GYMRMYWAKRLLDWTPSPAAAYRAAVLLNDRWELDGRDPNGYAGIAWAICGKHDRPWP
                     PERRVLGFIRPMSASGAARKFDVDAYIARVERA"
     gene            753..2273
                     /locus_tag="VFR25_05260"
     CDS             753..2273
                     /locus_tag="VFR25_05260"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HET9940490.1"
                     /translation="MSQLPLWHGALGQRASLRPLSKSKIAAGYQCPKRLYLEAHHLAE
                     RDPLDPGRAAILDAGRRVGLVARRRYPSGVRVEEDPRYHDRAVAETRAALEDPRVDAI
                     YEAAFTFDDIRVRVDILARNDGAWDLVEVKSSTGFKEEYVVDLATQVHVVEGSGLRVA
                     RAMLLHVNSRYLWPGGPYDVNSLFSLLDLSREARAQVPRLLDRVARMREVLRSLEEPD
                     VAIGPHCRKPYRCPYYGHCHTGMSPHHISRLPRLTPRIYQALVHAGVLDIREIPEEFE
                     GLNELQVRVHRAAKSGLPFAHPDLHAQLGAIRYPVHFLDFETCNPALPVIPGTHPFQQ
                     VPFQWSDIVLEEDGAIRHREYLHADRTDPRPPLARALVEALEAGGSIVVYSGFEGRVI
                     RGLAGEIPELAPRLLPLTDRLVDLHRLILDGYYHPEFHGSFSIKNVLPVLVDGLGYDD
                     LVIREGSQAALAFISMTDPEVPASDRAKLREGLSAYCKRDTEAMLRLFQVLTKERP"
     gene            2270..2947
                     /locus_tag="VFR25_05265"
     CDS             2270..2947
                     /locus_tag="VFR25_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940491.1"
                     /translation="MTKRWGWRWCAALVLAAAMSQATAGAATHPREKSWSWTFEADTL
                     GKEPNNSGLATGTWEVASLASDSLAADGAAGGRVLRQLEGEDGADYHMLQLKKPIVND
                     LVASVRFRVVSGEVDPGAGLLFHLDDKARNGYLIRVSGAKGRVIAHYIIYGRRRDIRY
                     ASIEPPKPGEWHTISVNKKKSVIVVSYDGTECLRFRDERFKRGTVGFWTEDDAVVDFA
                     DLKVAMN"
     gene            complement(3222..>3890)
                     /locus_tag="VFR25_05270"
     CDS             complement(3222..>3890)
                     /locus_tag="VFR25_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9940492.1"
                     /translation="KVLVPGFYDEVEIPGAEERKAINAKPWNEESVKRALGVKRLFHE
                     EGFTHVESGTIRPTLQVNGIRGGYIGEGFKTVIGAKASAKISMRLVHNQDPEKIYERF
                     ERFVRELVGDMATVEFKRFGAGQPFRERLENPLVQAGMKALEAGFGKPAIGMWMGGSI
                     PIVAPLVAITRAPCVLMGFGLPGDNLHAPNENFPIACFIGGARSATAYLNEVGLRAGA
                     LAKK"
BASE COUNT          601 a         1346 c         1302 g          641 t
ORIGIN      
        1 ctcgccacgc tccccgtcga ccgatcggcc gcgccggtgc ccggcgcgcg cggtgggacc
       61 gcggccggtc ttcgagccct ccggctgttc ctgaaggggc ctctccagcg ctaccacctc
      121 gaccggaaca ggccgccgat cgacggcacg agccggctct cggcctatct ccattttggg
      181 cacctgggcc cgcagacggt ggcgctcgcc gtccgcagcg ccccggcccc ccgggccgcg
      241 aagcaagcct atctcgagga gctgatcgtc cggcgtgagc tggcggtgaa cttcgtcgcg
      301 aggaacgcgc agtacgacga cgtggcttcc gctccggact ggggacgccg cacgctcgaa
      361 cgtcacgcgc gggacccgcg cgaggagatc tactcctggg acgctctcga agcgggggcg
      421 acgaaggatc ggctctggaa cgcggcgcag cgcgagatgg tggccacggg acggatgcac
      481 ggatacatgc gcatgtattg ggcgaagcgg ctcctcgact ggacgccttc acccgccgca
      541 gcgtatcgcg cggccgtgct cctgaacgat cgatgggagc tcgatggccg cgacccgaat
      601 ggttacgccg ggatcgcctg ggccatctgc ggcaagcacg atcgtccgtg gccgccggag
      661 cgccgggtgc tcggcttcat ccgccccatg agcgcctctg gcgccgcgcg gaaattcgac
      721 gtcgacgcat acatcgcacg ggtggagcgg gcatgagcca gctgcccctc tggcacggcg
      781 ccctcggaca gagggcctcg cttcggcccc tctccaagtc caagatcgcg gccggctacc
      841 agtgcccgaa gcgcctctat ctcgaagccc atcatctcgc ggagcgcgac ccgctcgatc
      901 ccgggcgcgc ggcgatcctc gatgccggac gccgcgtggg cctggtggcg cgacgccgct
      961 acccgagcgg cgtgcgagtc gaagaggatc cccgctatca cgaccgggcc gtcgcggaga
     1021 cgcgcgccgc gctcgaggat cccagggtcg acgcgatcta cgaggccgcg ttcaccttcg
     1081 acgacatccg ggtgcgcgtc gacatcctcg cgcggaacga cggcgcttgg gatctcgtcg
     1141 aggtgaagtc gagcacggga ttcaaggagg agtacgtcgt cgatctggcg acgcaggtcc
     1201 acgtggtcga ggggtcgggc ctgcgcgtgg cgcgggcgat gctgctccac gtgaactcgc
     1261 gctacctctg gccgggcggt ccgtacgatg tgaattcgct cttctcgctg ctcgatctct
     1321 cgcgggaggc gcgcgcccag gtgccgcggc tcctcgatcg ggtggcgagg atgcgcgaag
     1381 tcctccgctc gctcgaggag ccggacgtgg cgatcgggcc tcactgccgg aagccgtacc
     1441 gctgccccta ctacgggcac tgccacaccg gcatgtcgcc gcatcacatc agccggctcc
     1501 ccaggctcac gccgaggatc taccaggccc tcgtccacgc gggcgtcctc gacattcgcg
     1561 agatcccgga agagttcgag ggattgaacg agctccaggt ccgcgtccac cgggcggcga
     1621 agtcgggact cccgttcgcg catcccgatc tccacgcgca gctcggggcg atccgttatc
     1681 ccgtccactt cctcgatttc gagacctgca atccggcgct gccggtgatt ccgggcacgc
     1741 atccgttcca gcaggttccc ttccagtggt ccgacatcgt gctcgaggag gatggcgcca
     1801 tccgtcaccg cgaatacctg catgcggacc ggaccgatcc gcggcctccg ctcgctcgcg
     1861 cgctcgtcga ggctctggag gccggaggct cgatcgtggt ctactccggc ttcgaggggc
     1921 gggtgatccg cggtctcgcg ggggagattc cggagctcgc gccgcggctc ttgccgctca
     1981 cggaccggtt ggtcgatctg catcggctga tcctggacgg ctactaccat cccgagttcc
     2041 acggctcgtt ctcgatcaag aacgtgctcc ccgtgctggt ggacgggctc gggtacgacg
     2101 atctcgtgat tcgcgaggga agccaggccg cgctcgcgtt catctcgatg accgatcccg
     2161 aggttcctgc ttcggatcgc gccaagctca gggaagggct gtcggcctac tgcaagcgcg
     2221 acaccgaagc catgctgcga ctcttccagg tgctgacgaa ggagcgtcca tgacgaagcg
     2281 gtggggatgg cgatggtgcg cggctctcgt tctggcggcg gcgatgtccc aggctacggc
     2341 cggggcggcg acgcaccccc gcgagaagag ctggtcctgg acgttcgaag ccgacacgct
     2401 ggggaaggag ccgaacaact cgggtctggc cacggggacc tgggaagtgg cgagcctcgc
     2461 gtccgattct ctggccgcgg acggggcggc cggggggcgc gtcctccgtc agctcgaagg
     2521 agaggacggc gccgactacc acatgctcca gctgaagaaa ccgatcgtga acgacctcgt
     2581 ggcctccgtc cgcttccgcg tggtatcggg agaggtggac cccggggccg ggctcctctt
     2641 ccacctggac gacaaggccc ggaacggcta cctgatccgg gtgagcgggg cgaaggggcg
     2701 cgtcatcgcg cactacatca tctatggaag gcgccgcgac atccgctacg cctcgatcga
     2761 gccgccgaag ccgggcgagt ggcacaccat ctccgtcaac aagaagaaga gcgtgatcgt
     2821 cgtgagctac gacgggacgg agtgcctgcg attccgggac gagcgcttca aacgggggac
     2881 ggtcggcttc tggaccgagg acgacgcggt cgtcgacttc gcggatctca aagtcgcgat
     2941 gaactgatcc acgagaggcg gcggattcgg ggaagggtcc tgccggagtg gagggggcgc
     3001 gccgtcacgg tggggcctga gtcgggtgcc ggtgagaacc gcgagctcac accatgccgc
     3061 cgtacgcgcc ccgtcgtcca tgacttgggg tactgccgtc cggccgtcca tggccgggtg
     3121 gcgcaccttg gcactgcatt agatgcggcc aaccgcgcgg acgataccgc agcactgcgc
     3181 ctcgcgctat ctccttttgt gaccgggcct cccggccccc gtcatttctt ggccagggcc
     3241 cccgcccgga gccccacctc gttcaggtag gccgtggccg atctcgctcc gccgatgaag
     3301 caggcgatcg ggaagttctc gttcggcgcg tggaggttgt cgccgggtaa accgaatccc
     3361 atcaacacac aaggggcgcg ggtgatcgcc accaggggcg ccacgatcgg aatcgaccct
     3421 cccatccaca tcccgatcgc cggctttcca aatcccgcct cgagggcctt cattcccgcc
     3481 tgcaccaacg ggttctcgag ccgctcccgg aacggctgcc cggcgccgaa ccgcttgaac
     3541 tccacggtcg ccatgtcgcc caccagttcc cgcacgaatc gctcgaaccg ctcgtagatc
     3601 ttttccggat cctggttgtg cacgaggcgc atgctgatct tggcgctcgc cttcgcgccg
     3661 atcacggtct tgaagccctc gccgatgtat ccaccccgga tgccgttaac ctgaagcgtc
     3721 ggacgaatcg tgcccgattc gacgtgcgtg aagccctcct cgtggaacag ccgtttcacg
     3781 ccgagcgccc gtttgacgga ctcctcgttc cagggcttcg cgttgatcgc cttgcgctct
     3841 tccgcgccgg ggatctcgac ctcgtcgtag aagcccggca ccagcacctt
//
LOCUS       DASTKC010000128         6137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_805225, whole genome shotgun sequence.
ACCESSION   DASTKC010000128 DASTKC010000000
VERSION     DASTKC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805225"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2549
                     /locus_tag="VFR25_05275"
     CDS             <1..2549
                     /locus_tag="VFR25_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HET9940493.1"
                     /translation="ACLAALWSHSFAATPVPSSDPLVAGEAAWEAGDYDEARAYFESA
                     LDLADTRKGAADPNAAEALGRIGALLFEQGECREAITYDRRALAILDAAEAGSLKRAE
                     ALARLGAALSACGDARGAERERTRALEAWKDAKAESTRAAAACLVDLAGDCLKLGKTT
                     EARDHLLRARGILERLYPPGDPRVASTILGLGEVAEAEGDYDRARAFYREAVAIRRRS
                     LGSDHPSLAEALADHARALIRTGNMGDAVEASLEAESIRRAYLRYTVRALPERQALSY
                     ESRLPSSLGVAVAAGVRTWDSTLVERVWDSVVRSRGVVLDEMALRQHWSDELGDTVSD
                     DLVTAWTGARERLAHLAVRGPLDEPPDVHRAALDSLKAQSERAEAALVARSALRAEGR
                     RADAGFRDVVAKLPPGTALVSLVRYDDPLAAGASPSDSMLAQGGSASYAAFVTRGRGQ
                     PAWAIPLGSAVEIDSLVTLWRNQVSRPAKGDDALGAAERRYSGRLRQRIWDPIEARLK
                     GVTRIVLIPDGSLHLVNPIALRREDGRFLVEDGPVFQIVSSERDLIGPATVGRPGAGF
                     LGIADPDFDTPPTDSASVRETGDLDSGPGDEREVSRGLPCSEGGSRPQWERLPGSARE
                     VEEAAALWQRALGTGARDTGVDDVQHPPSSPQARVLLGRAASEDAFRRLAAGRSILHV
                     ATHGFFADACPAWEAAADSASPDNPLLGAGLVLAGANLPIAKGAAPGQDGILTADEIA
                     AVDLSGVETAVLSGCETGVGVVRAGEGVFGLRRAFEIAGVRTLVLSLWPVTDRAARAW
                     TTRFYQARLIGGEDAAAAARDASRGVLEARRAAGESTDPIYWAAFIAAGR"
     gene            complement(2572..3756)
                     /locus_tag="VFR25_05280"
     CDS             complement(2572..3756)
                     /locus_tag="VFR25_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET9940494.1"
                     /translation="MRFPRTHAVLAAVLLAAFAALASGAAQPGAKAADPAPQPADTPA
                     IALTPVGRFDFVAPSSEDRALSPEELSGIAWIEGSHYIAIGDEHATMHRLEIDLDHET
                     GRIKGVHFEKAIPLRGEDGVLIPDSLGSDREGIEFNPENDTVCISNEHTGADQSRPSI
                     ACHRMDDGRTTRLIRTDGSDMMKVFSTQRLNGGFESITRRPDGGEIWTANEMPLTVDS
                     EPPSDTTGAVVRLQKFDAGMKPVAQYAYRLDAYPQKIAMPPKLRGYAVNGLSELLMLP
                     GGILLALERTFAGDASGLPNLRIRIYQVDIDGATDVSGIGPDVGLHRAKYMPASKRLL
                     WEKNFGLTNSNFEGMAIGPQLRNGDLPLILVADNNGGDDEAFYSLRISGLAPREASAP
                     RR"
     gene            complement(3822..5099)
                     /locus_tag="VFR25_05285"
     CDS             complement(3822..5099)
                     /locus_tag="VFR25_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9940495.1"
                     /translation="MNAILAIALKDLKLLARDKSGFFWVLLFPLLIALFFGSVFAGGE
                     SRAPMPIAVVDQDGSAFSHSMGAELAKSEALKVHGMSLDSARTAVRQGRLTAYVLFPK
                     GSGQSLGFGSTNDSSSIELGLDPSRRAEAGYLKGLVTQALFSSLMGSFTMEAPGREAI
                     RERIASIEADTTIAATRRGELATFYRNLDQMLGSLDRVQSTAPDSAAPRNAGPRIRTR
                     DMTAEAAEPRSAFEVTFPSGVMWALIGVCMSFAISIVHERLTGTLLRLRLAPISRTQV
                     LLGKALAAFLAAMGATGLLLTFASLVLGVRITNPAALLAALAASAFCFVGIMMVISTL
                     GRTHQAVSGAGWAILLVMSMTGGGMIPLIAMPSWMVMLSHFSLVKWGVLAIEGAIWRG
                     FSWSEMALPLGILVAVGALGLAIGARRLERAQS"
     gene            complement(5096..5842)
                     /locus_tag="VFR25_05290"
     CDS             complement(5096..5842)
                     /locus_tag="VFR25_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940496.1"
                     /translation="MPAAAIQVEDLHHAYGAVPALNGVSFQVSQGECFGLLGPNGAGK
                     TTTIQAIVGALRPDRGRVAILGEDDPTRPSVRSRIGVVPQSIALYQELTGEENLAFFG
                     KLYGLNGARLKTRVDATLALAGLTDRAKDRASTYSGGMQRRLNLVCGLIHDPLLILMD
                     EPTVGVDPQSRNLIFENIESLKAQGRTVLYTTHYMEEAERLCDRVAIVDHGKILALDR
                     VSALLTLSPTPSPDLEAVFLHLTGTTLRDR"
     gene            5932..>6137
                     /locus_tag="VFR25_05295"
     CDS             5932..>6137
                     /locus_tag="VFR25_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940497.1"
                     /translation="MRLRSLVLGAVVGLPAAAVFGVAFAAPAALAASVAPAAPAAPAA
                     SAVPAASATPAVPAAPRVFALRFAR"
BASE COUNT          885 a         2052 c         2265 g          935 t
ORIGIN      
        1 tcgcgtgcct ggcggcgctt tggtcccatt cgttcgccgc gactcctgtg ccttccagcg
       61 acccgctcgt cgcgggcgag gccgcgtggg aagcggggga ctacgacgag gcacgcgcgt
      121 acttcgagtc cgcgctcgat ctggccgaca ctcgaaaggg cgccgccgat ccgaacgccg
      181 ccgaagcgct cggtcggatc ggcgctctgt tgttcgagca gggcgagtgc cgcgaggcca
      241 tcacatacga tcggcgcgcg ctcgcgattc tcgatgccgc ggaagcgggc tccctgaaac
      301 gcgcggaggc tttggcccgg ctcggcgcgg ccctctccgc ctgcggcgat gcgcgaggcg
      361 cggagcggga gcgaacgcga gcgctcgagg catggaagga cgccaaggcg gaatccactc
      421 gcgccgcggc cgcctgcctc gtcgatctcg cgggcgattg cctgaagctc ggcaagacca
      481 cggaagcgcg cgatcatctc ctccgggcgc gcggcatcct cgagcgcctg tatccgccgg
      541 gcgatccgag agtcgcgtcg acaatcttgg gattggggga ggtcgccgag gccgaggggg
      601 actacgaccg ggcgcgcgcg ttctatcgcg aggcggtcgc cattcgccgc cgctcgcttg
      661 ggtcggatca tccttctctc gccgaagccc tggccgatca cgcgcgcgcg ctcatccgca
      721 ccgggaacat gggggacgcg gtcgaggcct cgctcgaggc cgagtcgatc cgccgcgcgt
      781 acctgcgcta caccgtgcgc gcgctcccgg agcggcaggc gctctcgtac gagagccggc
      841 ttccgtccag cctcggcgtc gcggtcgcgg cgggagtgcg cacgtgggac tccacgctcg
      901 tcgagcgcgt gtgggattcg gtcgtgcgat cgcgcggcgt ggtgctggac gagatggcgc
      961 tgcgccagca ctggagcgac gagctgggcg acaccgtgtc cgacgacctc gtcaccgcgt
     1021 ggaccggcgc gcgggagcga ctcgcgcacc tcgccgtgcg cggaccgctc gacgagccgc
     1081 ccgacgtcca tcgggccgcc ctcgacagcc tgaaagctca gagcgagcgc gccgaggcgg
     1141 ccctcgtcgc gcgaagcgcg ctccgcgccg aaggacgtcg tgcggacgcg ggattccggg
     1201 acgtggtcgc gaagcttccg ccaggaaccg cgctcgtgag tctcgtccgg tacgacgacc
     1261 ctctcgccgc gggcgcttcg ccctcggatt cgatgctcgc gcaaggaggc tcggcgtcct
     1321 acgcggcctt cgtcacgcgc ggccgcgggc agcccgcctg ggccatcccg ctcgggagcg
     1381 ccgtcgagat cgattcgctc gtgacgctct ggcgcaatca ggtgtcgcgt ccggccaaag
     1441 gggacgacgc gctgggcgcg gcggagcggc gctactcggg ccggctccgt cagcggatct
     1501 gggatccgat cgaggcgagg ctcaagggcg tgacgcgcat cgtcctgatc cccgacggtt
     1561 cgctccatct cgtcaatccg atcgcgttgc gacgcgagga cggccggttc ctcgtcgagg
     1621 acggccccgt gttccagatc gtctcgtcgg agcgcgatct catcggtccg gcgaccgtcg
     1681 gccgtcccgg cgccggattt ctcgggatcg cggatcccga cttcgatacg ccgccgaccg
     1741 actccgcgtc cgtgcgtgag accggcgacc tggactccgg gcccggcgac gagcgcgagg
     1801 tgagccgcgg gcttccgtgc tccgagggcg gatcgcgtcc gcagtgggag cggctcccgg
     1861 gcagcgcgcg tgaagtcgag gaagccgccg cgctctggca gcgcgcgctg gggacgggag
     1921 cgcgcgatac gggtgtcgac gacgtgcagc atccgccgtc ctcgccgcaa gcgcgcgtcc
     1981 tcctcggacg cgcggcgagc gaggacgcgt tccgccggct cgcggcgggg cggagcatcc
     2041 tccacgtcgc gacgcacgga ttcttcgccg acgcctgtcc cgcgtgggag gccgcggcgg
     2101 acagtgcgtc gccggacaat ccgctcctcg gtgctgggct cgtactcgcg ggagcgaacc
     2161 tcccgatcgc gaagggggcc gcgccgggcc aggacggaat cctgaccgcg gacgagatcg
     2221 cggcggtcga tctctcgggt gtggagaccg cggtgctttc cggttgcgag accggcgtcg
     2281 gagtggtgcg cgccggcgaa ggcgtattcg gtctacggcg cgcgttcgag atcgcgggcg
     2341 tgcggacgct ggtgctgagt ctctggcccg tcaccgaccg cgcggcgcgt gcgtggacga
     2401 cgcgctttta ccaggcgcgc ttgatcggag gggaggacgc tgccgccgcc gcgcgggacg
     2461 cgagtcgcgg cgtgctcgag gctcgcaggg cggcggggga gtcgaccgat ccgatctact
     2521 gggccgcgtt catcgccgcg ggacggtaat caccgcggag ggtagcctcg cctagcgccg
     2581 cggggcgctg gcttcgcgcg gggctagccc cgagatccgc agcgagtaga aggcttcgtc
     2641 gtcgccgccg ttgttgtcgg cgacgaggat caaaggcaga tctccgtttc gaagctgcgg
     2701 gccgatcgcc attccctcga agttcgagtt ggtgagaccg aagttcttct cccagaggag
     2761 ccgcttcgag gccggcatgt acttcgcccg gtgcaggccc acgtccgggc cgatccccga
     2821 cacatccgtc gctccgtcga tgtccacctg gtagatccgg atgcgcaggt tcggaagtcc
     2881 cgacgcgtcg ccggcgaacg tccgctcgag cgcgaggaga atgcccccgg gcagcatgag
     2941 gagctcggag agcccgttca ccgcatagcc gcgcagcttg gggggcatcg cgatcttctg
     3001 cgggtaggca tcgagccggt acgcgtactg ggcgaccggc ttcatcccgg cgtcgaactt
     3061 ctgaagacgc acgacggcgc ccgtcgtgtc gctcggaggc tcgctgtcga ccgtcagcgg
     3121 catctcgttg gcggtccaga tctcgccgcc gtccgggcgc cgcgtgatcg attcgaagcc
     3181 gccgttcagc cgctgcgtgg agaacacctt catcatgtcc gacccgtcgg tgcggatgag
     3241 ccgggtcgtg cggccgtcgt ccatgcggtg gcacgcgatg gacgggcggc tctggtcggc
     3301 tccggtgtgc tcgttcgaga tgcagacggt gtcgttctcc ggattgaact cgattccctc
     3361 gcgatccgag ccgagcgagt cgggaatgag cacgccgtcc tcgccgcgga gcggaatcgc
     3421 cttttcgaag tgcacgccct tgatgcggcc ggtctcgtgg tcgagatcga tctcgagccg
     3481 gtgcatcgtc gcgtgctcgt ctccgatcgc gatgtaatgg cttccctcga tccatgcgat
     3541 gccgctcagc tcctcgggtg agagggcccg gtcttccgac gagggagcga cgaaatcgaa
     3601 gcggccgacg ggggtgaggg cgatggcggg ggtatcggcc ggttggggcg cggggtcggc
     3661 ggccttggcg ccgggctggg cggctcccga ggcgagggcc gcgaaggcgg ccaggaggac
     3721 ggccgcgagc acggcgtggg tgcgtgggaa cctcaagcgg gacgcggcca tagcactcaa
     3781 ccgtaacccg gccgcggacc ctgccgcaag gcgccgcgct gctaagactg tgcgcgctcc
     3841 aagcgtcgcg cgccgatcgc gagtcccagg gcgccgacgg ccacgaggat gccgagcgga
     3901 agcgccatct cgctccacga gaagccgcgc cagatggcgc cctcgatggc gagcacgccc
     3961 catttcacga ggctgaaatg gctcagcatc accatccagg aaggcatggc gatgagcgga
     4021 atcatgccgc cgccggtcat cgacatgacg aggaggattg cccagcccgc gcccgagacc
     4081 gcctgatgcg tccgccccaa ggtcgagatc accatcatga tcccgacgaa gcagaaggcc
     4141 gacgccgcga gcgcggcgag gagcgccgcg ggattcgtga tccgcacgcc cagcacgagc
     4201 gatgcgaacg tgaggaggag cccggtcgcg cccatggcgg ccagaaacgc ggcgagcgcc
     4261 ttgcccagca gcacctgggt gcgcgagatc ggcgccaggc ggagacggag gagcgtgccg
     4321 gtcaggcgct cgtgcacgat cgagatcgcg aagctcatgc acacgccgat caaggcccac
     4381 atcacgcccg agggaaaggt gacctcgaat gcgctccgag gctccgccgc ttccgcggtc
     4441 atgtcgcggg tgcgtatgcg tggtcccgcg tttcgaggcg ccgcggagtc gggggctgtg
     4501 ctctgcacgc ggtcgagcga gcccagcatc tgatcgagat tgcgatagaa cgtggcgagc
     4561 tcgccgcgtc tcgtcgcggc gatcgtggtg tccgcttcga tcgaggcgat gcgctcgcgg
     4621 atcgcctcgc gccccggtgc ttccatggtg aaggagccca tgagggacga gaagagcgcc
     4681 tgcgtgacga gacccttcag gtagcccgcc tccgcccggc ggctcggatc gagccccagc
     4741 tcgatcgacg agctgtcatt cgtggagccg aagccgagcg actgccccga gcccttcggg
     4801 aagagcacgt acgccgtgag ccgcccttgg cgcaccgcgg tgcgcgcgga atcgagcgac
     4861 atgccgtgga ccttgagcgc ctccgacttc gcgagctcgg cgcccatcga gtgggagaag
     4921 gccgagccgt cctggtccac cacggcgatc ggcatcggag cgcgcgactc gcctccggcg
     4981 aagacggagc cgaagaagag cgcgatcagg agcgggaaga ggagcaccca gaaaaagccc
     5041 gacttgtcgc gcgcgaggag cttcaaatcc ttgagcgcga tcgcgaggat ggcgttcatc
     5101 ggtcgcggag cgtggtgccg gtgaggtgga ggaagaccgc ctcgaggtcg gggctcgggg
     5161 tcggggacag cgtgaggagc gcgctcacgc ggtcgagcgc gaggatcttg ccgtgatcga
     5221 cgatggccac gcgatcgcag aggcgctccg cctcttccat gtagtgcgtg gtgtagagca
     5281 cggtgcgtcc ctgtgctttg agcgattcga tgttctcgaa gatgagattg cgcgactgcg
     5341 gatcgacgcc gaccgtgggc tcgtccatca ggatgaggag tggatcgtgg atgaggccgc
     5401 agacgagatt gagccggcgc tgcatcccgc ccgagtacgt ggacgcgcgg tccttcgctc
     5461 gatcggtgag ccccgcgaga gcgagcgtcg cgtcgacgcg ggtcttgaga cgggccccgt
     5521 tgaggccgta gagcttcccg aagaaggcga ggttctcttc gccggtgagc tcctggtaga
     5581 gcgcgatcga ttgaggcacg acgccgatcc gggatcgcac cgacggacgg gtgggatcgt
     5641 cctcgccgag gatcgccacc cgaccgcggt ccggccggag cgcgccgacg atcgcctgga
     5701 tggttgtggt cttgccggcg ccgttcggac cgaggagtcc aaagcactca ccctggctga
     5761 cctggaacga gacgccgttc agcgcgggaa ccgcgccgta ggcatggtgg agatcctcga
     5821 cctggatcgc ggccgcgggc atggtgcggg agtatgcccc cggcgactca gggggccaac
     5881 cgtgaagatc agggttcccg ccggtccccg gcgggatgta gggttcccgc catgcgtcta
     5941 cgttcgcttg tgttgggggc ggttgtgggt ttgccggccg cggccgtctt cggggtcgcg
     6001 tttgccgcgc ccgctgcgct cgcagcatct gtcgcgcccg cggcgcccgc tgcgccggcc
     6061 gcatctgccg tgccggccgc ctccgcgacg ccggccgtgc cggccgcgcc gcgcgtgttc
     6121 gcgctccgct tcgcgcg
//
LOCUS       DASTKC010000129         7667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_806377, whole genome shotgun sequence.
ACCESSION   DASTKC010000129 DASTKC010000000
VERSION     DASTKC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806377"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFR25_05300"
     CDS             <1..1066
                     /locus_tag="VFR25_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HET9940498.1"
                     /translation="DVVRDALLREADRGGQSFFVHNRVESIDRMAHYLTSIVPQLRYG
                     VAHGQMRDTELERKMVDFLERRTDVLVSTMIIESGLDIPTVNTMLVNRADALGLAQVY
                     QLRGRIGRSHHQAFCYLLVPAGTVLTEEAEKRLRAISEHEELGAGLMIAMRDLEIRGA
                     GNLLGPEQHGFMATVGFDLYCRMVDEVVQELQGTAVARRPEPELSSDLPAFVPDDYVA
                     DRDEKLDVYRRLAAVAETTALDALRAELEDRFGPPPAEVSNLLHLKGLRLLGRDHGVE
                     RLRVGRDRLEIEVAEDLGRDRILSLVTSVPAPIEFAGKGSRTLRVKSPNDPLSLATNL
                     LQHLGPSDTVPRLPLPATGT"
     gene            1236..3071
                     /locus_tag="VFR25_05305"
     CDS             1236..3071
                     /locus_tag="VFR25_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9940499.1"
                     /translation="MVWTAWAVAALAATTPATTKTTTAAKAPAATSKTAPKAGAKKTA
                     ASPPKASGAVATVGTSRTIDQIDIQRAAQALYTDPLRVKDPAKWRRSLLDRCVDRELL
                     DMEAQRQGLDRDSALIALRNEREYSVFWREIYRRVLIPRLTPTEDELKAAYATSKYRR
                     VDLSVILIRTDLNPGQKPLAQRIATAGRGGARFDSLAKLYSEHPPTKAKGGRIGWVLM
                     KDVNPAWFEDAKKASEGAVLGPYVGPVGDEIYKIAAFQELTHDSLSHLIYFERSRSIT
                     DDYQREMLSKYHFAVDSTQMKGLIFAVASESPDSILKSLRPDGTRDKQGARPALGVLA
                     RCDGRTITFPDLLESTSADRREIGGIHLDSEDEVRALCARAVMEDLTSRDGRDRGIDR
                     DPAIARELRLIGEEVRTHEMVRRNTPSFDEASVRTYFDANPDRFKRPRTRRVKIVTFA
                     SPESAAVNAQAWRASGSIDDKVLAARNDFHETKLVTPWTASAGQYAVARYGTMDLPEG
                     SDDPLTRAVQGKAAGAVVGPIALPYGGHAVMQVLQVEDARPRTFDEARTLAHRLKREE
                     DESRWVDAELTRLRASTPVHILPGRLEATKLASHGNTTSTGGVAE"
     gene            3068..4384
                     /locus_tag="VFR25_05310"
     CDS             3068..4384
                     /locus_tag="VFR25_05310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9940500.1"
                     /translation="MNARRFLTAAIALTLLAWGGAPRVAPATERIAAVVNKEAILASD
                     VDEQTKDAAERMNVDPADTSTYNKLRGEVLGQLIEKQVLLTEATRQGITVTDAEVTQA
                     VDREILGLKQRIGSESDYQAALAREKTTEADLRKRYTPGVKEQLLISRLVGKEVQSKT
                     TVTDAEVKTYFEAHRDSIGRKPEELRMAHILIAFEPDTSQIRRAHARADSLRDLIVRK
                     KVPFEDVAQRFSDDPSARVGGDLGTFGHGDMVAEFEEVAFKLKPMEISTPVRTRFGYH
                     IIQVLEHFAKTDSTEERVHARHVMVQAKPTPADEDRARLRALALRDSILTKGADFAAV
                     ARKFSADTATKDSGGVLGAVPVPALPQNLREPLSGLAVGEVSVPFKRDAGYHLFKVLG
                     RTPETAYKYDDIKDDLRQVVLNKKLEESYRRWYDRVRKSVNVEVKN"
     gene            4404..5432
                     /gene="pdxA"
                     /locus_tag="VFR25_05315"
     CDS             4404..5432
                     /gene="pdxA"
                     /locus_tag="VFR25_05315"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET9940501.1"
                     /translation="MTTRRPTLVVTMGDPAGIGPEIVARAVASPAMRRTARLLVAGDL
                     EIMERAAKRTRARVRFEPAGAGGGGSGIPIVMSSQDGWQEIEPGHGTAASGRMAAQAI
                     EAAAELALQGGVDGIVTAPISKTALQAAGYPYPGHTEFLGALARVPETKMLFVGGPFR
                     ILLATVHLALKKVPDALTVDGLTRTIEFAAAAARNFRWKAMSAVAVLGLNPHAGEDGL
                     FGDEEARVIKPAIERARARGIRAEGPYPADTFFPQHAHRKDIGVVVAMYHDQGLIAAK
                     AGGIGGAANVTLGLPFVRSSVDHGTAFNRAWKGGKLAPDPAGLMTAIRIGADLASRAR
                     RPMEWSWR"
     gene            5423..6178
                     /locus_tag="VFR25_05320"
     CDS             5423..6178
                     /locus_tag="VFR25_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9940502.1"
                     /translation="MALIRLEHVSKHYGDRIALSDVTLSFDPGEWVFVLGHSGAGKTT
                     LLRILALAERPNSGRIEIDPEAVRRSPAVAVAEAAVGASGGTASEPPRRTKRIPVRRV
                     RRSVGMLGQEFRLLADRNVYENIAIACQISGIWDRAKIKDRVAPLLDQMGIRGKEKLY
                     PGDLSAGEKQRVALARAMARHPRILIADEPTGNLDPAAAGQIFALLREIASSGTLVAV
                     ATHSEEWIRRHPGRVIRLERGILRSDQMEGSAA"
     gene            6191..7060
                     /locus_tag="VFR25_05325"
     CDS             6191..7060
                     /locus_tag="VFR25_05325"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET9940503.1"
                     /translation="MGVLRYVAREFWRGFVSHRALWITSIASMASILLIFLLFLMVLR
                     SVESYTSHLESREEVSVFLNEGLSKTDVATVAASLQAIAGVDSVRYVSKDEAWESFRQ
                     DVGDEALVQAVGSNPLPASFVIRPGPGYRSADGVRSIAREAETVPHVEDVRFAGEWVL
                     RAQQVVSAVQKIAYALGLIVLLGVLFIVGSTTRLSVAARIDSIHLIRSLGGGFVFNEL
                     PYLVEGFVLAALSSAITVLVARALNNSLSEEMFRLQFLSTNDLLAFIGVSGLLGLLGS
                     WIAVATLPRKWLL"
     gene            7048..>7667
                     /locus_tag="VFR25_05330"
     CDS             7048..>7667
                     /locus_tag="VFR25_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940504.1"
                     /translation="MAALGAARPGLARLAVLLTALVFAVPVARAQAPPAPPDTSRAPA
                     RSAADTEIETQRRELESLKSQLDDRRKQGQALKGREKNVLVQLRESEKNLQLTVRYLN
                     ALEKRRRVVASSLGDATTELVRTAAQLETDRRRLAWRLREIYKRGRSADLEYLLSARS
                     FGDLVTRTYYLARIAQEDRGQVLLTQARRGQVQDTKTRLESRKREL"
BASE COUNT         1266 a         2640 c         2637 g         1124 t
ORIGIN      
        1 ggacgtggtg cgggacgctc tcctccgcga ggccgatcgc ggggggcagt ccttcttcgt
       61 gcacaaccgg gtcgagtcga tcgaccggat ggcgcactat ctgacctcga tcgtgcccca
      121 gctccgctac ggcgtcgcgc acgggcagat gcgggacacc gagctggagc gcaagatggt
      181 cgacttcctc gagcggcgga ccgacgtgct cgtctcgacc atgatcatcg agtcggggct
      241 cgacatcccg accgtgaaca cgatgctcgt gaatcgcgcg gacgcgctcg ggctggcgca
      301 ggtctaccag ctccgcgggc ggatcggacg gtcgcaccac caggcgttct gctacctgct
      361 cgtgcccgcc ggaaccgtgc tcaccgagga agcggagaag cggctccgcg ccatttccga
      421 gcacgaggag ctgggcgcgg ggctcatgat cgcgatgcgc gacctcgaga tccgcggcgc
      481 cggcaatctc ctcggccccg agcagcacgg gttcatggcg acggtgggct tcgacctcta
      541 ctgccgcatg gtcgacgagg tggtgcagga gctccagggg accgcggtcg cgcggcggcc
      601 ggagcccgag ctgtcgagcg atctcccggc gttcgtgccc gacgactacg tcgccgaccg
      661 ggacgagaag ctggacgtgt accggaggct cgcggcggtg gccgagacga cggcgctgga
      721 cgcgctccgc gccgagctcg aggaccgctt cgggccgccg ccggccgagg tgtcgaacct
      781 gctccacttg aaggggctcc gactcctcgg ccgcgaccac ggggtcgagc gccttcgcgt
      841 cgggcgggac cggctggaaa tcgaggtggc cgaggacctc ggacgggaca gaatcctgtc
      901 gctggtgacc tcggttccgg ccccgatcga gttcgccggg aagggctcga ggaccctccg
      961 ggtgaagtcc ccgaacgacc ccttgtcctt ggcgacaaac ctgttgcagc acttggggcc
     1021 ctctgatacg gtgccccgtc ttcctcttcc cgcgaccgga acgtgaaccg gggggcccga
     1081 ttggggtatc tgagggacga ggtcaggctc gcgttaccca ccgacgtcca ttccagggaa
     1141 tgatccagag tcgaacgccc cgcccatacc ggtcgaaccc tgccgtccaa ccttggtcga
     1201 agcgccgcct tccgcggctg ctcgtcgcga tcgccgtggt ttggaccgcc tgggccgtcg
     1261 ccgctctagc cgcaacgacc ccggcgacca cgaagaccac cacggcggcc aaggccccgg
     1321 ccgccacctc caagaccgcc ccgaaagcgg gcgccaagaa gaccgccgca tcgcccccca
     1381 aggcctccgg cgcggtggcc acggtcggca cgtcgcggac catcgaccag atcgacatcc
     1441 agcgcgccgc acaggctctc tacaccgatc cacttcgcgt gaaggatccg gcgaagtggc
     1501 gccgctcact cctcgatcgc tgcgtggacc gcgagctcct cgacatggag gcgcagaggc
     1561 aagggctcga tcgcgactcg gcgctcatcg cgctccggaa cgagcgcgag tactcggtgt
     1621 tctggcgcga gatctatcgc cgcgtgctca ttcctcggct cacgccgacg gaggacgagc
     1681 tgaaggccgc gtacgcgacc agcaagtatc ggagggtcga cctctccgtc atcctgatcc
     1741 gcacggatct caatcccggc cagaagccgc tcgcgcagcg catcgccacc gccggccgcg
     1801 gtggagcgcg attcgattcc cttgcgaagc tatacagcga gcatccgccc accaaggcga
     1861 aggggggacg gatcggctgg gtcctgatga aggacgtcaa tccggcgtgg ttcgaggacg
     1921 cgaagaaggc gtcggagggc gccgtcctgg gcccttatgt cggcccggtg ggcgatgaga
     1981 tctacaagat cgccgcattc caggagctga cccacgactc gctctcgcac ctcatctact
     2041 tcgagcggag ccgctccatc acggacgact accagcggga gatgctgtcc aaatatcact
     2101 tcgcggtcga ctcgacgcag atgaaaggtc tgatcttcgc cgtggcgagc gagagtcccg
     2161 attcgatctt gaagtcgctc cgtccggatg ggacgcgcga caagcagggc gcgcgcccgg
     2221 cgctcggggt gctggcgcgc tgcgacggac gcacgatcac ctttcccgac ctcctcgaga
     2281 gtacgtccgc ggatcggagg gagatcggcg ggatccacct ggattccgaa gatgaggtgc
     2341 gggcgctttg cgctcgcgcg gtgatggagg atctcacctc ccgcgacggg cgggaccgcg
     2401 gcatcgaccg ggatcccgcg atcgcgcgcg agctccggct catcggcgaa gaggtgcgga
     2461 cgcacgagat ggttcgccgc aacactccga gcttcgacga ggcctcggtg cgcacgtatt
     2521 tcgacgcgaa tcccgatcgc ttcaagcggc cccgcacgcg gcgcgtgaag atcgtgacct
     2581 tcgcgagtcc ggagagcgcc gccgtaaacg cacaggcatg gcgcgcttcg ggaagcatcg
     2641 acgacaaggt cctcgcggcg cggaacgact tccacgaaac gaagctggtg accccctgga
     2701 cggcttctgc cggccagtac gcggtcgctc gatacggtac gatggacctc ccggaaggaa
     2761 gcgacgatcc cctcacgcgc gcggttcagg gcaaggccgc cggcgcggtg gtcggaccga
     2821 tcgcgctccc gtacgggggc cacgccgtga tgcaagtgct ccaggtggag gacgcccgtc
     2881 cgagaacatt cgacgaggcg aggaccctcg cccaccgact caagcgggag gaggacgaga
     2941 gccgctgggt cgacgccgag ctgacccgcc ttcgtgcctc gacccccgtg catatcctgc
     3001 cgggccgtct cgaggccacg aagctggcta gccacggcaa caccacctca acgggaggcg
     3061 tcgctgaatg aacgcacgta ggttcctgac cgctgcgatt gcactgactc tgctcgcgtg
     3121 gggaggcgcg cctcgcgtcg cgccggccac ggagcggatc gcggccgtcg tgaacaagga
     3181 agcgatcctc gcgtcggatg tggacgagca gacgaaagac gccgcggagc ggatgaacgt
     3241 cgaccccgcg gacacctcga cctacaacaa gctgcgcggc gaggtgctgg gccagctcat
     3301 cgagaagcag gtcctgctga ccgaggcgac ccgccagggc atcacggtca cggacgcgga
     3361 agtcacccag gcggtggacc gcgagatcct gggtctgaag cagcggatcg gctccgagag
     3421 cgactaccag gccgccctcg cccgcgagaa gacgaccgag gccgaccttc ggaagcgcta
     3481 cacgccgggg gtgaaggagc agctcctcat ttcccggctc gtcggcaagg aagtgcagtc
     3541 gaagacgacc gtgaccgacg ccgaggtgaa gacctacttc gaggcgcacc gcgattccat
     3601 cgggcggaag cccgaagagc tccggatggc gcacatcctg atcgccttcg agccggatac
     3661 gtcgcagatc cgccgggcgc acgcgcgcgc ggacagcctt cgcgacctga tcgtgaggaa
     3721 gaaggtgccg ttcgaggacg tggcccagcg gttctcggac gatccgagcg cgcgggtcgg
     3781 cggcgacctc ggcacgttcg gccacggcga catggtggcc gagttcgagg aagtcgcgtt
     3841 caagctcaag ccgatggaga tctcgacccc ggtccggacg cggttcggat accacatcat
     3901 ccaggtgctc gagcactttg cgaagaccga ctccaccgag gagcgcgtcc acgcgcggca
     3961 cgtcatggtg caggccaagc ccacgcccgc cgacgaggac cgcgcgaggc tccgggcgct
     4021 cgcgctccgg gattcgatcc tcaccaaggg ggccgatttc gcggccgtcg cgcggaagtt
     4081 ctcggccgac acggccacga aggactcggg cggcgtgctg ggggccgttc ccgtgccggc
     4141 gctgccgcag aatttgcgcg agcccctgag cgggctcgcg gtgggcgagg tgagcgtccc
     4201 cttcaagcgg gacgcggggt atcatctctt caaagtactg ggccgcacgc ccgagaccgc
     4261 ctataagtac gatgacatca aggacgatct ccgccaggtg gtcctcaaca agaagctcga
     4321 ggagagctac cggcgctggt acgaccgcgt ccgaaagagc gtgaacgtcg aagtaaagaa
     4381 ctgaacccgg agcgggacca cccatgacca cgagacgccc cacgctcgtc gtcaccatgg
     4441 gtgatcccgc ggggatcggg cccgagatcg tggcccgcgc ggtcgcgtcg cccgcgatgc
     4501 ggcgcacggc gcggctcctc gttgcgggcg atctcgagat catggagcgc gccgccaagc
     4561 ggacgcgcgc cagggtccgc ttcgagcccg cgggcgcggg tgggggtgga agcggcattc
     4621 cgatcgtcat gtcgagccag gacggctggc aggagatcga gccggggcat gggacggcgg
     4681 cttcgggacg catggccgcg caagccatcg aagcggcggc ggagctcgcg ctccagggag
     4741 gggtggacgg gatcgtgacg gctccgattt ccaagacggc gctccaggcg gccggctatc
     4801 cctatcccgg acacaccgag ttcctgggcg cgctcgcacg cgtgccggag acgaagatgc
     4861 tcttcgtcgg cggccccttc cggatcctcc tcgccaccgt tcatctcgcg ctcaagaagg
     4921 tccccgacgc gctgaccgtg gacgggctca cgcgcacgat cgagttcgcc gccgccgccg
     4981 cgcgcaactt ccgctggaag gcgatgagcg cggtcgcggt cctgggcctc aatccgcacg
     5041 ccggcgagga cggactcttc ggcgacgagg aagcgcgcgt catcaagccc gcgatcgagc
     5101 gggctcgcgc gcgcggcatc cgcgcggaag gcccctatcc ggccgacacc ttcttcccgc
     5161 agcacgctca tcggaaggac ataggcgtgg tcgtggcgat gtaccacgac caggggctca
     5221 tcgccgccaa ggcgggaggc atcggcggcg cggccaacgt aacgctcggg ctcccattcg
     5281 tgcgaagctc cgtggatcac ggcaccgcgt tcaaccgcgc gtggaaggga gggaagctcg
     5341 cccccgatcc cgccggactc atgaccgcga tccggatcgg cgcggacctc gcgtcgcgcg
     5401 cccgtcgccc catggagtgg tcatggcgct gatccgtctc gagcacgtga gcaagcacta
     5461 cggcgaccgg atcgccctga gcgacgtcac gctctccttc gatccgggcg agtgggtctt
     5521 cgtgctcggc catagcggcg cggggaagac gacgctcctt cgcatcctcg cgctcgcgga
     5581 gcggccgaac tcgggacgca tcgagatcga tcccgaagcc gtgcgccgct cgccggctgt
     5641 agccgtggcc gaggcggccg tcggagcctc gggaggcacg gccagcgagc ccccccgacg
     5701 gacgaagcgc atcccggttc gccgcgtgcg ccggtcggtc ggaatgctgg ggcaggagtt
     5761 ccggctcctc gcggatcgga acgtctacga gaacatcgcc atcgcctgcc agatctcggg
     5821 gatctgggac cgcgcgaaga tcaaggatcg ggtcgcgccg cttctcgacc agatgggaat
     5881 tcgcggcaag gagaagctct acccgggcga cctctcggcc ggcgagaagc agcgcgtggc
     5941 gctcgcgcgc gcgatggcgc gccacccgcg catcctgatc gccgacgagc cgaccggcaa
     6001 cctggacccg gcggcggcgg gacagatctt cgcgctcctt cgcgagatcg cctcgagcgg
     6061 gaccctcgtc gcggtggcga cccactccga ggaatggatc cgccgccatc cgggccgcgt
     6121 catccgtctc gagcgcggca tcctgcgatc cgaccagatg gagggaagcg ccgcgtaagg
     6181 cgcgagagcc atgggcgttc ttcgctacgt cgcgcgggaa ttctggagag gcttcgtctc
     6241 ccaccgcgcg ctctggatca cgagcatcgc gtcgatggcg tcgatcctcc tcatcttcct
     6301 cctcttcctc atggtgctcc ggagcgtcga gagctacacg tcgcatctcg agtcccgtga
     6361 ggaagtgtcg gtcttcctga acgaggggct ttcgaaaacg gacgtcgcca cggtcgccgc
     6421 gtcgctccag gccatcgccg gcgtcgattc ggtccgctac gtgagcaagg acgaagcgtg
     6481 ggagtccttc cggcaggacg tgggggatga agcgctggtc caggcggtgg gatcgaatcc
     6541 gctccccgcg tcgttcgtga tccggcccgg acccggctac cggagcgccg acggcgtgcg
     6601 gtccatcgcg cgcgaagcgg agaccgtgcc ccatgtggag gacgtgcgct tcgcggggga
     6661 atgggtgctc cgggcgcagc aggtggtgag cgcggtccag aagatcgcct acgcgctcgg
     6721 gctgatcgtg ctcctgggcg tgctcttcat cgtcggttcg acgacgcgcc tctccgtggc
     6781 cgcgcgcatc gactcgatcc acctcatccg gtcgctcggc ggcggattcg tgttcaacga
     6841 gctgccctat ctcgtcgagg ggttcgtgct cgccgcgctc agctccgcca tcacggtgct
     6901 cgtggcgcgc gcgctcaaca actcgctctc cgaggagatg ttccggctcc agttcctctc
     6961 gacgaacgat ctcctggcct tcatcggcgt gagcggcctc ctcggactcc tgggaagctg
     7021 gatcgcggtg gccacgctcc cgcggaaatg gctgctctag gggcggcccg cccgggcctc
     7081 gcgcgtctcg cggtcctttt gaccgcgctc gtcttcgctg tgcccgtagc tcgagcgcag
     7141 gcgccccccg cgccgcccga cacgtcgcgc gcgccggcgc gaagcgccgc ggacacggag
     7201 atcgagacgc agcggcgcga gctcgagtcc ctgaagtcgc agctcgacga tcggcgcaag
     7261 cagggacagg cgctcaaggg gcgcgagaag aacgtcctgg tccagcttcg cgagagcgag
     7321 aagaacctcc agctcacggt ccgctacctg aacgcgctcg agaagcgccg ccgcgtcgtc
     7381 gcctcgagcc tcggcgacgc gaccaccgag ctcgtgcgca cggccgcgca gctcgagacc
     7441 gaccgccgcc gcctcgcctg gcggcttcgc gagatctaca agcgtgggcg gagcgcggat
     7501 ctcgagtacc tgctctccgc gcgctccttt ggcgacctcg tgacccgcac ctactatctc
     7561 gcgcgcatcg cgcaagagga ccgcggtcag gtgctcctca cccaggcgcg ccgcggtcag
     7621 gtgcaggaca cgaagacgcg cctcgagtcg aggaagcgcg agctgga
//
LOCUS       DASTKC010000130         9474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_808338, whole genome shotgun sequence.
ACCESSION   DASTKC010000130 DASTKC010000000
VERSION     DASTKC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808338"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VFR25_05335"
     CDS             <1..1114
                     /locus_tag="VFR25_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET9940505.1"
                     /translation="IFYNQAIMSAEGIPQVAAVLGMCTAGGAYVPAMSDENVIVKGAG
                     TIYLAGPPLVKAATGEEVTPEDLGGAEVHSRVSGVTDHMAANEEDALHTVRDIVERLN
                     TVKRVDLDLAEPVDPHYPAEELYGILPEDTKQPYDVREVIARLVDDSRLHEFKSNYGT
                     TLVCGFARWMGYPVGIVANNGVLFSESALKGAHFVELCSQRKIPLIFLQNITGFMVGK
                     RYESGGIAKDGAKMVQAVATAEVPRITVLIGASHGAGNYAMCGRGYRPRFLFTWPNSR
                     ISVMGAEQAASVLTQVKRDQLAREGKTLSAAEADAIAKPVLEKYELEGSPYYATGQLW
                     DDGIIDPADTRAVVALALSASLNAPVTRGPAPVYRM"
     gene            1154..1957
                     /locus_tag="VFR25_05340"
     CDS             1154..1957
                     /locus_tag="VFR25_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9940506.1"
                     /translation="MSSESRLRVERDGAVARVTLARPEVRNAFDDVLIAELIRVLEDL
                     RDRANADPDQAPRAVVLTGEGTAFCAGADANWMRRSVTYTREENEADARRMAGMLRSL
                     DELPIPVIARVNGACLGGGMGLIACCDIVVAAEAADFGFTETRLGIAPAVISSFVLPK
                     IGAAAARRYFLTAEIFKAPVARDIGLVHDVVPLEGLDAAVERLVGALRGCGPRAVAAA
                     KRLIRENLALTRDEAIENSVRAIAGLRASAEGQEGLGAFLAKRAPAWKS"
     gene            1954..3423
                     /locus_tag="VFR25_05345"
     CDS             1954..3423
                     /locus_tag="VFR25_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9940507.1"
                     /translation="MTPITKVLVANRGEIAIRICRTLRTMGIPSVVVFSEADRGAPHT
                     KAGDEAVLLGAAEPAQSYLNVDRILGAARATGASAIHPGYGFVAENAAFARACAAAGV
                     TFIGPSPESMERLGSKTKAREAAIRLKVPVVPGVEDVPDAARARQEADRIGYPVLLKA
                     ASGGGGRGMRRVAKAEEMDGAFESGRREAEASFGDGRLLLEKLVHPARHIEVQILADG
                     KDAIAFGERECSLQRRYQKIVEESPSSAVTPKTRAKLEEAAITLARDARYSGAGTVEF
                     LLGPDGAFYFLEVNTRLQVEHPVTEARFGLDLVRAQIEIAAGGKLPKRPEPKGHAIEV
                     RLNAEDPYRGFIPQSGPISLLAWPPGEGIRVDAGIEEGHPVHAHYDSLLAKVIAYGKD
                     RSEARERLIQALRALALLGIRTNQGFLIDLLASEAFATGETYTHTVESSSWPPPDQVP
                     DEALLAAALALEAPRAALGGRRDADRWSPWRLLGAWGRA"
     gene            3420..3935
                     /locus_tag="VFR25_05350"
     CDS             3420..3935
                     /locus_tag="VFR25_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9940508.1"
                     /translation="MSRKVTFRWEGHAIEGSIETRGNRATWTRDAEALDAEIVRDGAW
                     VEIRTTAGASRAAVKAGPKEVWVSLCGRTYVLERAHRTSSGSAAATSNDEIRAPMTGK
                     VVRVTAAAGDQVKEDDLLLTIEAMKMEFKLTAPEDARIEEILCADGDRVELGQLLVRL
                     APLVEPAKGAS"
     gene            3932..4879
                     /locus_tag="VFR25_05355"
     CDS             3932..4879
                     /locus_tag="VFR25_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET9940509.1"
                     /translation="MNAEARDPMTQVRIVEVGPRDGLQNEKDLIPTETKVAFVDALSE
                     AGYQDIEVSAFVSPTWVPQLADAAEVFARIRRREGVRYSALVPNEKGLDRALEARVQA
                     VAVFTAASETFNQKNINASIAESIARFKQVVPRAKQEGLFVRGYVSTAFWCPYEGKIG
                     PRAVTDVVRRLLDLDVDEISIGDTIGKAVPAEVDDLLDVLLDAIPQERVAMHFHDTYG
                     TAVANALAAYDRGITVFDSSAGGVGGCPYAPGAAGNIATEDLVWALTRSGASTGIDLA
                     RVSAASDLLASALGRPLRSRVREALSPSTAPSSAPERRS"
     gene            complement(4896..6008)
                     /locus_tag="VFR25_05360"
                     /pseudo
     CDS             complement(4896..6008)
                     /locus_tag="VFR25_05360"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
     gene            complement(6020..7342)
                     /locus_tag="VFR25_05365"
     CDS             complement(6020..7342)
                     /locus_tag="VFR25_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9940510.1"
                     /translation="MPSARRVHPLLIVLLACVIAAPRALASQHGDPVVPLLLALTVVL
                     VSAKIGGWIATRVRQPAVLGELVAGILIGNLGLVGFHGFEPFQTDTTLSILAGLGVIL
                     LLFEVGLDSTVLQMMQVGASALLVALLGVVTPFALGWLAGAWLLPDRPSYVHAFLGAV
                     LTATSVGITARVLRDLDAARTSEAKIILGAAVIDDVLGLVILAVVSGVITAAGHGASG
                     VSLPAIAWVCAKAIGFLAGAILLGRLLAPWLFRVASLVRVRGILLTTGLIFCFLLSYL
                     AALADLAPIVGAFAAGLVLEGSHLERFEEAKETTLESLLHPISTFLVPIFFVMTGMRV
                     SLETFADPAVIGLASALIVVAWVGKQACSLGVLQKGVDRLTVGLGMVPRGEVGLIFAA
                     MGATLTLAGQPVIGPATYSAIVIMVIVTTLVTPPALKWSIERQRRRAA"
     gene            7431..8126
                     /locus_tag="VFR25_05370"
     CDS             7431..8126
                     /locus_tag="VFR25_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940511.1"
                     /translation="MTRLPRFLASTASFVAACAVASSVTAAPSPPPRQEPPPPPTSTN
                     SSLLGDTLSASSAKSAARTQAETYYAAGYKDVEKAKKDKEAGKANDAKKRFGKARGKF
                     EEAVKLDPDYFEAWNMLGYSARQSGDLKASFAAYDKCLAIKPDYDEAHEYRGEAYLMN
                     GQIDLAKQELAWLKAEGSDEAEELAEAIEKVEGKKAGVKEEGAKTEATSESKESETKP
                     AEVKSETKAPEAK"
     gene            complement(8130..8933)
                     /locus_tag="VFR25_05375"
     CDS             complement(8130..8933)
                     /locus_tag="VFR25_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HET9940512.1"
                     /translation="MKSPVTIRPGRSFQIEDLPTTDVAGFKDKADGRGQLEKHLERLR
                     ELQHLLYADGRHAVLIVLQAMDTGGKDGTIRHVMSGFNPAGCMVTSFKVPSDEERKHD
                     FLWRIHHAVPPKGVIGVFNRSHYEDVLVVRVHKMVPKEVWKARYKQINEFERMLTETG
                     TTVIKFFLHISKKEQAKRLKARIEDKKKNWKFSSADLAERKLWSEYQDAYEDALRECS
                     TEWAPWTVVPADVKWARDEMVARVMVERLETLDLRYPKPQKDLDLVVVK"
     gene            complement(9041..>9474)
                     /locus_tag="VFR25_05380"
     CDS             complement(9041..>9474)
                     /locus_tag="VFR25_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET9940513.1"
                     /translation="GLDPIHNYMDYSTDICYTNFTAGQDTRMNAAVNTYRSWIGNVRV
                     ANGASTSQNAAAAVTAFRAVPNPFNPRTKIEFGLRTGTQVSVRVYDIQGRLVATLVNR
                     ELPAGNHSVDFNGEKLASGVYMMVLHVKGEAPVVRRMSLLK"
BASE COUNT         1564 a         3203 c         3300 g         1407 t
ORIGIN      
        1 gatcttctac aaccaggcga tcatgtccgc cgaagggatc cctcaagtag cggcggtgct
       61 cgggatgtgc accgcgggcg gcgcctacgt tccggccatg tcggacgaga acgtcatcgt
      121 gaagggcgcg ggcacgatct atctcgcggg gccgccgctc gtgaaggcgg cgacgggcga
      181 ggaggtgacg cccgaggacc tgggcggcgc cgaggtccac tcgcgcgtga gcggggtcac
      241 cgatcacatg gcggcgaacg aggaggacgc gctccacacc gtgcgcgaca tcgtcgagcg
      301 cctcaacacg gtcaagcgcg tcgacctcga tctggccgag cccgtggacc cgcactatcc
      361 cgccgaggag ctctacggaa tcctgcccga ggacacgaag cagccgtacg acgtccgcga
      421 ggtgatcgcc cgcctcgtcg acgacagccg gctccacgag ttcaagtcga actacggcac
      481 cacgctcgtc tgcggcttcg cgcggtggat gggctatccg gtcgggatcg tggcgaacaa
      541 cggcgtcctc ttctcggaga gcgcgctgaa aggggcgcac ttcgtcgagc tctgctcgca
      601 gcggaagatc ccgctcatct tcctccagaa catcaccggc ttcatggtgg ggaagcgcta
      661 cgagtcgggc ggcatcgcga aggacggcgc caagatggtg caggccgtgg ccaccgcgga
      721 agtgcctcgc atcacggtcc tgatcggcgc ctcgcacggg gccggcaact acgcgatgtg
      781 cggacgcggc taccgtccgc ggttcctctt cacctggccc aactcgcgca tctcggtcat
      841 gggggcggag caggcggcgt cggttctcac gcaggtcaag cgggaccagc tcgcgcgcga
      901 gggcaagacg ctctccgccg cggaagcaga cgccatcgcg aagccggtgc tcgagaagta
      961 cgagctcgag ggaagcccct actacgccac gggacagctc tgggacgacg gcatcatcga
     1021 tcccgccgac acgcgcgccg tcgtggcgct cgcactctcc gccagcctga acgcgcccgt
     1081 gacccgcggg cccgcacccg tctaccgcat gtgacccgcg ggccgcttcg gcccgcccga
     1141 ggaggaattc cacgtgagct ccgagagccg ccttcgcgtc gagcgtgacg gggcggtcgc
     1201 gcgcgtgacg ctcgcccgtc ccgaggtgag gaacgccttc gacgacgttc tgatcgcgga
     1261 gttgatccgc gtgctcgagg acctgcgcga ccgggcgaat gccgatccgg accaggcgcc
     1321 gcgggccgtc gtactgacag gcgagggaac cgccttctgc gcgggggccg acgcgaactg
     1381 gatgcgccgg agcgtgacct acacccgcga ggagaacgag gcggacgcgc ggcgcatggc
     1441 cggcatgctt cggagcctcg acgagctccc gatcccggtg atcgcgcgcg tgaacggcgc
     1501 gtgcctcggg ggcggcatgg ggctcatcgc ctgctgcgac atcgtggtgg cagccgaggc
     1561 cgccgacttc ggattcaccg agacccgtct cgggatcgcc cccgcggtga tctcgtcctt
     1621 cgtccttccc aagatcggcg ccgccgcggc gcggcgctat ttcctcaccg ccgagatctt
     1681 caaggctccg gtcgcgcgcg acatcggtct cgtgcacgac gtcgtcccgc tcgaggggct
     1741 ggacgcggcg gtcgagcggc tggtcggggc gctccgcggc tgcggccccc gcgcggtggc
     1801 cgcggcgaag cggctgatcc gcgagaacct cgcgctcacc agggacgagg ccatcgagaa
     1861 ttccgtccgc gccatcgcgg gtcttcgcgc atcggcggag ggacaggagg gcctgggcgc
     1921 cttcctcgcg aagcgcgccc ctgcctggaa gtcgtgacgc cgatcacgaa ggtcctcgtc
     1981 gccaaccggg gcgagatcgc gatccgcatc tgccgcacgc tccggacgat gggaatcccc
     2041 tcggtcgtgg tcttctccga ggcggaccgc ggagcgccgc acacgaaggc gggcgacgag
     2101 gccgtgctct tgggggcggc ggagcccgcc cagagctacc tcaacgtcga tcgcatcctc
     2161 ggggcggcgc gcgccacggg cgcgagcgcc atccatcccg gatacgggtt cgtcgcggag
     2221 aacgccgcct tcgcccgcgc ctgcgccgct gcgggcgtca cgttcatcgg cccctcgccc
     2281 gaatcgatgg agcgtctcgg atcgaaaacc aaggcgcgcg aggccgcgat tcggctcaag
     2341 gtccccgtcg tgcccggcgt cgaggacgtg cccgacgccg cgcgcgcgcg acaggaggcg
     2401 gaccgaatcg gctacccggt tctcctcaag gcggcgagcg gcggcggcgg acgcggcatg
     2461 cgccgcgtgg ccaaggcgga ggaaatggac ggcgcgttcg agagcgggcg ccgcgaagcc
     2521 gaagcgtcgt tcggcgacgg gcgcctcctc ctcgagaaat tggtccaccc cgcgcgtcac
     2581 atcgaagtgc agatcctcgc cgacggcaag gacgcgatcg ccttcggcga gcgcgagtgc
     2641 agcctccagc ggcgctatca gaagatcgtc gaggagtctc cctcgtcggc cgtcacgcca
     2701 aagacgcgcg cgaagctcga ggaggcggcg atcacgctcg cccgggatgc gcgctactcg
     2761 ggagcgggaa cggtggagtt cctgctcggc cccgacgggg cgttctactt tctcgaggtg
     2821 aacacgcggc tccaggtgga gcatccggtg accgaggctc ggttcgggct cgacctcgtg
     2881 cgcgcacaga tcgagatcgc cgccggcggg aagctcccaa agcgcccgga gcccaagggg
     2941 cacgccatcg aggtgcggtt gaacgccgag gatccgtatc gcggattcat tccgcagagc
     3001 ggcccgatct cgcttctcgc ctggccgccg ggagagggga tccgcgtgga tgcgggaatc
     3061 gaggaagggc atcccgtgca cgcgcactac gactcgctcc tcgcgaaggt gatcgcgtac
     3121 gggaaggatc gttcggaggc gcgcgaacgc ctgatccagg cgcttcgggc ccttgcgctc
     3181 ctcggcatcc gcacgaatca gggattcctc atcgacctcc tcgcgagcga ggcgttcgcg
     3241 accggagaga cctacaccca caccgtcgaa tcctcgtcgt ggcctccgcc ggatcaggtt
     3301 cccgacgagg cgctcctcgc ggccgcgctc gcgctcgaag cgccccgcgc cgcgctcggc
     3361 ggacggcgcg atgcggaccg ctggtccccg tggcggctgc tcggcgcatg gggacgcgcg
     3421 tgagccggaa ggtgaccttc cgctgggagg ggcatgccat cgaggggtcg atcgagactc
     3481 gagggaaccg ggccacctgg acgcgcgacg cggaggcgct cgatgccgag atcgtgcgcg
     3541 acggcgcgtg ggtcgagatc cgcacgaccg ccggcgcgtc ccgcgccgcc gtcaaggcgg
     3601 gacccaagga agtctgggtg tcgctctgcg ggcggacgta cgtgctcgag cgcgcgcatc
     3661 gaacgtcgtc cggctccgcg gccgcgacct ccaatgacga gatccgcgct cccatgaccg
     3721 gcaaggtggt gcgcgtcacg gcggccgccg gcgatcaggt gaaggaggac gatctcctcc
     3781 tcacgatcga ggcgatgaag atggagttca agctcacggc tccggaggac gcccgcatcg
     3841 aggagatcct ctgcgccgac ggcgaccgcg tggagctggg tcagctcctc gtccgcctcg
     3901 cgccgctcgt cgagccggcg aagggagcgt catgaacgcg gaagcgcgtg atcccatgac
     3961 ccaggtgcgc atcgtcgagg tgggcccgag ggacgggctc cagaacgaga aggacctgat
     4021 cccgaccgag accaaggtcg cgttcgtcga cgcgctctcc gaagcgggct atcaggacat
     4081 cgaggtgagc gcgttcgtgt cgccgacctg ggtgccgcag ctcgccgacg ctgcggaggt
     4141 cttcgcgcgg atccggcgcc gcgagggcgt ccgctactcc gcgctcgtgc cgaacgagaa
     4201 ggggctcgac cgtgcgctcg aggcccgcgt ccaggcagtg gcggttttca cggcggcgtc
     4261 ggagaccttc aaccagaaga acatcaacgc gtcgatcgcg gagtcgatcg cgcgcttcaa
     4321 gcaagtcgtg ccacgcgcca agcaggaggg actcttcgtg cgcggctatg tgtcgacggc
     4381 gttctggtgt ccgtacgaag ggaagatcgg gccgcgcgcg gtcacggacg tggtgcgccg
     4441 cctcctcgat ctcgacgtgg acgagatctc gatcggagat acgatcggaa aagcggtgcc
     4501 cgcggaggtg gacgatctgc tcgacgtgct tctcgacgcg attccgcaag agcgggtggc
     4561 gatgcacttc catgacacct acgggaccgc ggtcgcgaac gcgctcgccg cgtacgaccg
     4621 cgggatcacg gtcttcgact cctccgcggg aggcgtcggg ggctgtccct acgcgcccgg
     4681 cgcggcgggg aacatcgcga ccgaggatct cgtctgggcg ctcacgcgct ccggcgcttc
     4741 gaccgggatc gacctcgcgc gggtgtcggc tgcttccgac ctcctcgctt cggcgctcgg
     4801 gcggcctttg cgctcgcgcg tccgcgaggc gctttcccct tcgacggcgc cttcgtccgc
     4861 tcccgaacgt cgatcttgag ctcgtgcttg gcgatctcgc ggaggaaggc ctccgcggga
     4921 atcgcgcgct ccatcgcggc cacgccgcgg aacggcacct cgccgcgcgc gatcatgagg
     4981 gctgccgcag cggtgggata ggcggtcgtc cgcatcatcg cggtcacgcc ccgcttcgtg
     5041 tccatccggt ccagcatctc gaagacgatc tccttcttct tgccgtcctt cacgccttcc
     5101 acgatcacgc gcatcaccac gaggtcgggc tcgttcggga acgtgagcct cggcgccacg
     5161 accgccgcga agagcgagcg cggcgcgacc gacgcgcctt cgagcagcac gggcgtggtt
     5221 tcgaggaggc cgagatcccg gaagagccgg atcattgccg cgtgtccggg atagcgcacg
     5281 gtcttgtagt cgagtgtcgc gagcttgccc tcataggtcc agggcatggt ggacgtgccg
     5341 ccgagcgtca cgaaagcctc gcagcgtccg accggcttgg ggaactggag ctcctcgact
     5401 tcggtcaagg tctcgatctc ggtccgcttg ccgttcctga gcgcgacggc gcgtcccagg
     5461 tactcgttca ggagcccgtg catcgagaag accatcatgt attcgagcgg cggcttgggg
     5521 ttgagcggca gtccgcccac gcggatctgc acgaggtccg cacgatccag acgtccgatg
     5581 cccgcggcgg cgagcacatt ggccatgccc ggcgcgagtc cgcaatcggg gacgatcgtg
     5641 atatcggcgt cctgcgcctc gtcgttcaag gcctcctgcg cgcgaacgac gtcggtgttc
     5701 ccgccgaggt cgcaaaagga gacctgcgct tgaatcgcgg ccttcgtgag atccaggttc
     5761 aggaaatagg ggaccgcaga gatcacgacg tcgtagccgc ggaggcgctc gacgagccgg
     5821 ctctccctcg tgacgtcgat cgtctcggcg cgcacggtcg cgccgagccc ctggaggcgc
     5881 tccgccgcgc gcgccgcgcg ggcgccgtcc gagtcggcga ccacgacttc ctccacctcg
     5941 cgcgcgcgcg cgaggtccag cgccgcggca aagccttgca atcccgctcc cagaaccagg
     6001 aaacgcatcg cgctcctttc tacgcggccc gccgcctctg ccgctcgatg ctccacttga
     6061 gagcgggagg cgtgaccagt gtagtgacga tgaccatgat gacgatggcg gaataggtgg
     6121 ccggtccgat cacaggttgg cccgcaaggg tgagcgtggc gcccatcgcg gcgaagatga
     6181 gcccgacctc gccccgcggc accatcccga ggcccacggt cagccggtcg acccctttct
     6241 ggaggacgcc gagcgagcag gcctgcttgc cgacccaggc gaccacgatg agcgccgacg
     6301 cgagcccgat gacggccgga tcggcgaagg tttcgagaga aacgcgcatg ccggtcatca
     6361 cgaagaagat cggcacgagg aaggtcgaga tgggatggag cagcgactcg agcgtcgtct
     6421 ccttcgcttc ctcgaatcgc tccaggtgcg agccctcgag gacgagcccg gcggcgaacg
     6481 cgccgacgat cggcgccagg tccgcgagcg cggccaggta cgagaggagg aagcagaaga
     6541 tgagcccggt cgtgagcagg atgccgcgca cgcgcacgag cgacgcgaca cggaagagcc
     6601 atggcgcgag gagccgcccc aggaggatcg ctcccgcgag gaagccgatc gccttggcgc
     6661 agacccaggc gatcgccggc agggacacgc ccgacgcgcc gtggccggcg gcggtgatga
     6721 cgccgctcac cacggccagg atcacgaggc ccagcacgtc gtcgatgacg gcggctccca
     6781 ggatgatctt ggcctcgctc gtgcgtgccg cgtcgaggtc tcggagcacg cgcgcggtga
     6841 tgccgacgct ggtcgccgtg agcacggcgc cgaggaaggc gtgcacgtag ctcggccggt
     6901 ccggcaggag ccatgcgccg gcgagccatc cgagcgcgaa gggcgtgacc acgccgagca
     6961 gcgcgacgag gagcgccgac gcgcccacct gcatcatctg gagcacggtg gagtcgaggc
     7021 cgacctcgaa gaggagcagg atcacgccga gcccggccag gatcgagagc gtggtgtccg
     7081 tctggaacgg ctcgaacccg tggaatccga cgaggccgag attgccgatg aggatccccg
     7141 cgacgagctc tccgagcacc gccggctggc gcacgcgggt cgcgatccat ccaccgatct
     7201 tggccgacac gaggaccacc gtgagggcga gaaggagcgg aacgaccgga tctccgtgct
     7261 gcgaagcgag cgctctcgga gcagcgatca cgcacgcgag aagcacgatc aggagcgggt
     7321 ggacgcggcg ggcggaaggc atcgcggcag cataccgaaa gggagggggt tctggtagca
     7381 tcgagcctgg cgccgtaccg gcgctttgac cccatcgagg agagccttcc atgacccgcc
     7441 ttccgcgctt cctggccagc accgcatcct tcgtagccgc ttgcgccgtc gcgtcttccg
     7501 tgaccgcggc cccatccccg ccaccgcgcc aggagccgcc gccccctccg acgtcgacga
     7561 actcgtccct gctcggggac acgctgagcg cgtcctccgc gaagagcgcc gcccggaccc
     7621 aggccgagac ctactacgcc gcgggataca aggacgtcga gaaggccaag aaagacaagg
     7681 aagcgggcaa ggcgaacgac gcgaagaagc gcttcggcaa ggcgcgcggc aagttcgagg
     7741 aggcggtgaa gctggatccg gactacttcg aggcctggaa catgctcggc tactcggcgc
     7801 gccaatcggg agacctgaag gcctcgttcg cggcgtacga caagtgcctg gcgatcaagc
     7861 ccgactacga cgaggcccac gagtatcgcg gcgaggccta tctcatgaac gggcagatcg
     7921 atctcgccaa gcaggagctg gcgtggctca aggccgaagg ctcggacgag gcggaggagc
     7981 tggcggaggc gatcgagaag gtcgagggca agaaggcggg cgtgaaggag gagggcgcca
     8041 agacggaggc gacgtccgaa tccaaggaat ccgaaacgaa gccggccgag gtcaagtccg
     8101 agacgaaagc tcccgaggcg aagtagccgc tacttcacga cgaccagatc gagatccttc
     8161 tgcggcttgg gatagcgcag gtcgagggtt tctagcctct ccaccatgac gcgggccacc
     8221 atctcatccc gcgcccactt cacgtcggcc gggaccaccg tccatggcgc ccactccgtg
     8281 gagcactcgc ggagcgcgtc ctcgtacgcg tcctgatact cgctccagag cttccgctcg
     8341 gccaggtccg cggacgagaa cttccagttc ttcttcttgt cctcgatgcg cgccttgagc
     8401 ctcttcgcct gctctttctt gctgatgtga aggaagaact tgatgaccgt ggtccccgtc
     8461 tcggtgagca tgcgctcgaa ctcgttgatc tgcttgtagc gggccttcca gacctccttc
     8521 ggcaccatct tgtgcacgcg gaccaccagt acgtcctcgt agtgcgaccg gttgaagacc
     8581 ccgatcactc ccttgggcgg cacggcgtgg tggatgcgcc agaggaagtc gtgctttcgc
     8641 tcctcgtcgg agggaacctt gaacgatgtg accatgcatc ccgccggatt gaatccgctc
     8701 atgacgtggc ggatggtgcc gtccttgccg cccgtgtcca tggcctggag cacgatcagc
     8761 accgcgtgcc gcccatccgc atagaggaga tgctggagct cgcggagccg ctctaagtgc
     8821 ttctcgagct ggccgcgccc atccgccttg tccttgaagc ctgcgacatc cgtggtcggg
     8881 agatcttcga tctggaacga gcgtccgggg cggatcgtca cgggcgattt cacgggggcc
     8941 tccgggacag ggaagaacgg gaagtatgtc cccccatcat agcagcgggg ccgatccttg
     9001 cggaccggcc ccgtttccct gcgatgcggt cgagcgaacg ctacttgagg agcgacatcc
     9061 tccgcaccac cggtgcctcg cccttcacgt ggagcaccat catgtagacg ccgctggcga
     9121 gcttctcgcc gttgaagtcc accgagtggt ttccggccgg gagctcgcgg ttcacgagcg
     9181 tcgcgaccag gcgcccctgg atgtcataca cgcgcaccga cacctgcgtg cccgtgcgca
     9241 ggccgaactc gatcttcgtg cgcggattga acggattcgg aaccgcgcgg aacgcggtga
     9301 ccgccgcggc cgcgttctgg ctcgtcgaag ctccgttcgc cacgcggacg ttgccgatcc
     9361 aggagcggta ggtgttcacc gcggcgttca tgcgggtgtc ctggccagcg gtgaagttcg
     9421 tgtagcagat gtccgtggag taatccatgt agttgtggat cggatcgagc cccg
//
LOCUS       DASTKC010000131         7847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1992892, whole genome shotgun sequence.
ACCESSION   DASTKC010000131 DASTKC010000000
VERSION     DASTKC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992892"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1722)
                     /locus_tag="VFR25_05385"
     CDS             complement(58..1722)
                     /locus_tag="VFR25_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9940514.1"
                     /translation="MDVRTVPELLCRAAERHPKPDQFCIKREGRWIQLPLDHVLRRAV
                     WVMRALRERGIKHGDRVVILAETSIEWVIADVAILSIGAVTVPIYPTLPASQIAPLIA
                     DSGSRGAFASTAELKAKLEQAGVAASGLEWIWCWKDEPFPEPGEEAYAGGDAAPEDLA
                     SIIYTSGTTGVPKGVMLTHANIVAESLLSLRAMSVQESDVYLSFLPLSHVYERCAGLY
                     TMIDAGATIAYAESIDRMAANLQECRPTIMLAVPRFYEKFYARVMEASAAAGFPIAPM
                     FAWARRVAIAWATRRNERRSVGPGLALQHALAKALVYRKLGARLGGRLRLRVSGGAAL
                     NREVALFFYGVGQPIHEGYGLSETASAICVNRFGDQRIGTVGTLFDEIEVKFAEDGEI
                     LVKGPVVMKGYWNRPEETAKALEGGWFHTGDIGTIDPDGHIRITDRKKDIIVTSGGKK
                     VAPQLIENALKESPRIHEVLVLGEGQKYIAALIVPEGSATREEIAPDVEHVNSGLANF
                     EKVRKFEVIPNDLSVANGTLTPSLKLKRQAVADKHRDLIQRLFAEH"
     gene            1787..3088
                     /locus_tag="VFR25_05390"
     CDS             1787..3088
                     /locus_tag="VFR25_05390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9940515.1"
                     /translation="MTSALMRDVILTDGFRTPFAKAGTALAPVPARELGRIAVTELLA
                     RHGVDPLEVDEVILGNVAAPSDATNIARVVALLAGIPERVPAVTVQRNCASGMEAVAQ
                     AHDRIAAGHADLIVAGGTESMSQIPLFVSEGMTRVFEKLSRARSLPARVAAIAQARPR
                     DLAPRPAILEGLTDRVSGLNMGETAEVLAKEFGISREAQDDFALTSHRRAIAAMDAGR
                     LAEEIVPVFAPPYKAAVLEDVGPRRNQSLEALAKLRPYFDRRHGTVTPGNSCGITDGA
                     AALLVVSAEKARELGLRPAGRIRGQAAVGLDPAHMGLGPTHATPIALERAGAHFKDLG
                     LIEINEAFAAQVIANEIAMASAKYCREKLGLDGPIGELDRSVLNVNGGAIALGHPVGV
                     SGTRLLLTLMREMRHRDVQLGLAALCVGGGQGVAMVVERAA"
     gene            3085..5250
                     /locus_tag="VFR25_05395"
     CDS             3085..5250
                     /locus_tag="VFR25_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9940516.1"
                     /translation="MRAAIREREPDTHPSEFGPFRLEAHPSGVVTLWFDDHDRKVNLL
                     DSRSLDSLQRALAALKLRKTPPRALVLASGKEGQFIAGADVKEFDALTSPAQAEAKAK
                     DAQELFDEIAKLGYPTVAAIDGPCLGGGTELVLACRHRIASDARSTAIGLPEVRLGIL
                     PGFGGSQRLPRLVGLPQALNLILTGRSLDCYRARRIGLVDDVLPHERFVHRVVDWTES
                     MLKQSRPPRRKGPPLQDWLLGALPPLRALVLSQARKTALRQTGGHYPAAPEIIRVLGA
                     TSGKSLTEGLRIERKAVAKLLFTPESINLRRIFSMGEEAKRIPPAPRAYPVDFTAVFG
                     AGTMGGEIAYLFTRIGLRVRLRDIKPEPILRSLEHAQSLFAREVSQRRITRAEMERAM
                     DRIEPALDLGGLARVDVVLEAVIEDLGIKQSLLRDVEAETSEHAVLATNTSSLSVKAM
                     ARSLKRPGRLVGMHFFNPAARMPLVEVIRTDDTDQDALDTVLALTRRIKKTPVLVRDA
                     PGFLVNRVLMPYLSEAVGLVERGSDIELIDKELKRFGMPMGPLALLDEIGLDVARKVA
                     HVLEEAFGSRIGSTALLDRLVAAGALGKKSGLGFYRYDKAQRDGINPSLRDLSSKNAP
                     VPPDEIKNRLLDAMVNEAALALDERVVDRPEDVDLAMVMGTGFPPFRGGLLRYADASG
                     IGGFVERLARRQQEGAPSGPCGKLQRMALGNERFYPTRD"
     gene            5295..5933
                     /locus_tag="VFR25_05400"
     CDS             5295..5933
                     /locus_tag="VFR25_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940517.1"
                     /translation="MPNVMGVLRAEIRRLARKETKQELSSMKKQLTQTKRLLAESRRR
                     ISDLESRAKHVARATRSSAVAREDEEDGPQVRFSPKWVKKHRSKLGMSRLVYAQLVGV
                     SPQTIMGWESGRTRPRRGALKTWRAIRAKGVRELRSMLANGGEVSPGAKRRVRRRLKR
                     AVRRARVRGAIRRVRVRRAVKRARVRRAVRKVRTRVARTVRRARTRRAAKRK"
     gene            5947..6723
                     /locus_tag="VFR25_05405"
     CDS             5947..6723
                     /locus_tag="VFR25_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9940518.1"
                     /translation="MKDARDLAREQFGKNPERYVSSPVHAKGASLDRIVELLAGRGHE
                     TVLDIATGGGHTALAVAPHVARVIATDLTPEMLEAARGFLTSRGVANVEFRVADATSL
                     PFTDGEFDAVTCRIAPHHFPDVRAFLREVYRVLKPGGVAIVVDNVVPEDESAAAFINE
                     FEKTRDPSHVWAYPPSGWVRFFEEAGFLDLQVESFFKALDFEAWTDLKDMDPATKSRL
                     RDMIVEAPPKALDALFPEDRDGALWFYLQELLIRGVKPGS"
     gene            complement(6737..7234)
                     /locus_tag="VFR25_05410"
     CDS             complement(6737..7234)
                     /locus_tag="VFR25_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940519.1"
                     /translation="MKRHLVAGFVVLVYVALGIAGAALRGGVDPAIAGAGATPKTSSA
                     TKLPATGDVCPPGSGKHAVTVRGEIVDYYCYIEKGLTGPQHKDCGLKCVAGDVCMGIL
                     TEQGDLYMLSIDHLRAMAPLSFQGVPDPFKKCMGMVSEHVEIRGSLMERKGQRILEVA
                     AVRKV"
BASE COUNT         1303 a         2621 c         2637 g         1286 t
ORIGIN      
        1 gcccaggccg gctcggcgat gcacagcaac gcgagcaaga gccagaaacg gggcaggtca
       61 gtgctccgcg aagaggcgct ggatgagatc gcggtgcttg tccgcgacgg cctggcgctt
      121 cagcttgagc gagggtgtaa gggtgccgtt ggccacggac agatcgttgg ggatgacctc
      181 gaacttgcga accttttcga aattggcgag gcccgagttc acgtgctcca cgtcgggggc
      241 gatctcctcg cgcgttgcgg atccctcggg aacgatgagc gccgcgatgt acttctgccc
      301 ttcgcccagc accagcactt cgtggatccg cggcgattcc ttgagcgcgt tctcgatcaa
      361 ctgcggcgcg accttcttgc cgccggacgt gacgatgatg tccttcttgc gatcggtgat
      421 gcgaatgtgt ccgtcgggat cgatggtgcc gatgtcgccc gtgtggaacc atcccccttc
      481 cagcgccttc gccgtttcct cggggcggtt ccagtagccc ttcatcacga cgggaccctt
      541 cacgaggatc tcgccgtctt cggcgaactt cacctcgatc tcgtcgaaca gcgtgcccac
      601 ggttccgatc cgctggtcgc cgaagcggtt cacgcagatc gcggacgcgg tctcggacag
      661 accgtaccct tcgtggatcg gctgcccgac gccgtagaag aacaacgcca cctctcgatt
      721 gagcgcggcg ccgccggata cgcgcagccg caaccgcccg ccgaggcgcg cacccaactt
      781 cctgtaaacg agcgccttcg ccagagcgtg ctgtagcgcc aacccggggc ctacgctgcg
      841 tcgctcgttc ctccgcgtag cccacgcgat cgccacgcga cgcgcccacg cgaacatggg
      901 agcgatgggg aagccggccg ccgccgaggc ctccatgacg cgggcgtaga acttctcgta
      961 gaagcgaggc accgccagca tgatcgtcgg ccggcactcc tggagattcg cagccatgcg
     1021 gtcgatggat tccgcgtagg cgatcgtcgc acccgcgtcg atcatggtgt agagaccggc
     1081 gcagcgctcg tacacgtggc tcagggggag gaacgacaaa tagacgtccg attcctgaac
     1141 cgacatcgcc cgtagcgaca gcagcgattc cgcgacgatg ttcgcgtgcg tgagcatgac
     1201 gcccttgggg acgcccgtcg tgcccgacgt atagatgatg gacgccagat cctcaggggc
     1261 cgcatcgccg ccggcatacg cctcttcgcc gggctcggga aagggctcgt ccttccagca
     1321 ccagatccat tccagtccag acgccgcaac gcccgcctgt tcgagcttgg ccttgagctc
     1381 cgccgtggac gcgaacgcgc cgcgactccc cgaatcggcg atcaggggcg cgatctgcga
     1441 ggcgggcaag gtgggataga tcggaacggt gacggcgccg atcgagagga tcgcgacgtc
     1501 cgcgatcacc cactcgatgc tggtctcggc caggatcacg acgcgatcgc cgtgcttgat
     1561 gccgcgctcg cgcagcgcgc gcatcaccca aacggcgcgc cgcaagacgt gatccagcgg
     1621 gagctgaatc cagcggcctt cgcgtttgat acaaaactgg tccggcttag gatgccgttc
     1681 cgcggcccga caaagcagct cgggcaccgt acgaacgtcc attcgttgac ctccgatggc
     1741 ccctgtgaca aactagctga aagcgtcgta cgttgataca caactcatga cgagcgccct
     1801 catgcgggac gtgattctca cggatggatt ccggaccccc tttgcgaagg cgggaaccgc
     1861 gctcgcaccg gtgccggcgc gggagctggg gcgcatcgcc gtcaccgagc tcctcgcgcg
     1921 ccacggcgtc gatccgctcg aagtggacga ggtcatcctc ggcaacgtcg ccgcgccttc
     1981 cgacgccacc aacatcgccc gtgtcgtcgc gcttctcgcc ggaatccccg agcgcgtccc
     2041 cgccgttacg gtgcagcgaa attgtgcatc gggtatggag gcggtggcgc aggcgcacga
     2101 tcgcatcgcg gcgggccacg ccgacctgat cgtcgcgggc ggcaccgaat ccatgagtca
     2161 gatccccctc ttcgtgagcg aagggatgac ccgcgtcttc gagaagctct cgcgcgcccg
     2221 gtcactcccg gctcgcgtcg ccgcgatcgc gcaagcacgc cccagggatc tcgccccgcg
     2281 tccggccatc ctcgagggtc tgaccgatcg cgtctccggg ttgaacatgg gagaaaccgc
     2341 cgaggttctg gccaaggaat tcggcatcag ccgcgaggcc caggacgact tcgccctcac
     2401 gagccatcgg cgggccatcg ccgcgatgga tgcgggacgg ctcgcggagg agatcgtccc
     2461 cgtcttcgcg ccgccgtaca aagccgcggt gctcgaagac gtcggaccgc ggcggaacca
     2521 gtcgctcgaa gcgctggcca agctgcgccc ctacttcgat cgccgccacg gcacggtgac
     2581 gccggggaac tcctgcggca tcacggacgg ggcggcggcc ctcctggtag tgtccgcgga
     2641 gaaggcgcgc gagctcggac tgaggccagc gggaaggatt cgcggccagg ccgcggtcgg
     2701 tctcgatccc gcgcacatgg gactcggccc gacgcacgcg accccgatcg cgctcgagcg
     2761 cgccggcgcc cacttcaagg atcttggact catcgaaatc aatgaggcgt ttgccgctca
     2821 agtcatcgcg aacgagatcg cgatggcttc ggccaagtat tgcagggaga agctgggcct
     2881 cgatggtccg atcggcgagc tggaccggtc cgtcctcaac gtgaatggcg gcgccatcgc
     2941 gctcggccat cctgtcggcg tctcgggcac gcgcctcctc ctcaccctca tgcgcgagat
     3001 gcggcatcgc gacgtccagc ttggattggc ggcgctctgc gtcggtggcg ggcagggagt
     3061 cgcgatggtc gtggagcgcg ccgcgtgaga gcggccatcc gggagcgcga gccggacacg
     3121 cacccgtccg agttcgggcc gttccggctc gaggcgcacc cgtccggggt cgtcaccctc
     3181 tggttcgacg accacgaccg caaggtgaac ctcctcgact cgcggagcct cgactcgctc
     3241 cagcgcgcgc tcgccgcgct caagctccgg aagacgccgc cgcgcgcgct cgtgctcgcg
     3301 tcgggcaagg aagggcagtt catcgccggc gccgacgtga aggaattcga cgcgctcacg
     3361 tcgcccgcgc aggccgaagc caaggccaag gacgcgcagg agctcttcga cgagatcgcg
     3421 aagctcgggt atcccaccgt ggcggcgatc gacggtcctt gcctcggcgg cggtacggag
     3481 ctggtgctcg catgccgtca tcgcatcgcc tcggacgcgc gctcgacggc aatcgggctg
     3541 cccgaggtgc ggctcggaat cctgcccgga ttcggtggat cgcagcgcct gccgcgcctc
     3601 gtgggattgc ctcaggcgct caacttgatc ctcaccgggc gctccctcga ttgctaccgc
     3661 gcgcgcagga tcggactcgt ggacgatgtg ctgccacacg agcggttcgt gcaccgcgtg
     3721 gtggattgga ccgaatcgat gctgaaacag tcccgtccac cgcggcgaaa agggccgccc
     3781 cttcaagatt ggctgctcgg agcgcttccg ccgctccggg cgctcgtgct gagccaggcg
     3841 aggaagacgg cgctccggca gacaggcggc cactatccgg ctgcccccga gatcatccgc
     3901 gtactcgggg cgacatccgg caagtcgctc accgaggggc tccgcatcga gcgaaaggcg
     3961 gtcgcaaagc tcctcttcac gcccgagtcg atcaacctcc ggcggatctt ctcgatgggc
     4021 gaggaggcga agcggattcc gccggcgccg cgcgcctatc ccgtggactt cacggccgtc
     4081 tttggagcgg ggaccatggg aggcgagatt gcgtatctct tcacgcgcat cggcctccgc
     4141 gtgcggcttc gcgacatcaa gccggagccg atccttcgct cgctcgagca cgcgcagtcg
     4201 ctcttcgcgc gtgaggtctc gcagcgccgc atcacccgtg cggagatgga gcgcgcgatg
     4261 gatcgaatcg agccggccct cgacctgggc ggactcgcgc gcgtggacgt ggtgctcgag
     4321 gcggtgatcg aggacctcgg catcaagcaa tcgctccttc gcgacgtgga ggcggagacg
     4381 tccgagcacg cggtgctcgc gacgaacacc tcgtcgcttt cggtcaaggc gatggcccgg
     4441 agtctcaagc ggccggggcg cctcgtgggg atgcacttct tcaaccccgc ggcccgcatg
     4501 ccgctggtcg aggtgattcg caccgacgac accgaccagg acgccctcga cacggttctc
     4561 gcgctgacgc ggcggatcaa gaagacgccg gtcctcgtgc gcgacgcgcc cggtttcctc
     4621 gtgaaccgcg tgctcatgcc ctacctgagc gaagccgtgg ggctcgtgga gcgggggagc
     4681 gacatcgagc tcatcgacaa ggagctgaag cgcttcggca tgccgatggg cccgctcgca
     4741 ctcctcgatg agatcggact cgacgtcgcc cgcaaggtgg cgcacgtgct cgaggaggcg
     4801 ttcgggagcc ggatcggctc gacggcgctc ttggatcggc tcgtagccgc gggcgcgctc
     4861 ggcaagaaat cgggcctcgg cttctatcgc tacgacaagg cgcagcgcga cgggatcaat
     4921 ccgtcgcttc gcgatctgag ctcgaagaac gctccggtgc cgccggatga gatcaagaat
     4981 cgtctgctcg acgccatggt gaacgaagcc gcgctggctc tcgacgagcg cgtggtcgat
     5041 cggccggaag acgtcgacct ggccatggtg atgggaaccg gattcccgcc gttccgcggc
     5101 gggcttctcc ggtatgccga tgcctcgggc atcggcggat tcgtggagcg gctcgcgcgg
     5161 cgccagcagg agggagcgcc gtccggtccc tgcggcaagc tccagcgcat ggcgctcggc
     5221 aacgagcggt tctacccgac acgagactga tcgctccgcc ttcgggcgga gcttcccttt
     5281 ggaaggagga tgacgtgccg aacgtgatgg gcgtgcttcg cgccgagatc cggcgcctcg
     5341 cgcgcaagga gacgaagcag gagctgagct ccatgaagaa gcagctcacc cagacgaagc
     5401 gactgctggc cgagtcgcgg cgccgcatca gcgatctgga gtcgcgggcg aagcacgtcg
     5461 cgcgggcgac gcgcagcagt gcggtcgctc gcgaggacga ggaggacggg ccgcaggttc
     5521 gattctcgcc caagtgggtg aagaagcacc ggagcaagct cggcatgagc cggcttgtct
     5581 acgcgcagct cgtcggcgtg tcgccgcaga ccatcatggg gtgggaatcg gggcgcacgc
     5641 gcccacggcg cggcgccctc aagacatggc gtgccattcg agccaagggc gtgcgcgagc
     5701 tccgctccat gctggcgaac gggggcgagg tctcgccggg ggcgaagcgc cgcgtgcgtc
     5761 gccgcctgaa gagagccgtc cgtcgagcgc gcgtgcgcgg ggcgattcgc cgcgttcgtg
     5821 tgaggcgcgc ggtcaagcgc gcccgggttc gccgcgccgt ccggaaggtg cgcactcgtg
     5881 tggcccgcac ggtccgccgc gcccgcacgc ggcgcgccgc caagaggaag taggctcgcg
     5941 acgacgatga aggacgcgcg cgacctcgct cgcgagcaat tcgggaagaa tccggaacgc
     6001 tacgtctcga gcccggttca tgccaaggga gcgagcctcg accgcatcgt cgagctcctc
     6061 gccgggcgcg gccacgagac cgttctcgac atcgcgaccg ggggcgggca cacggcgctc
     6121 gccgtggcgc cgcacgtcgc gcgcgtgatc gcgaccgatc tcacgcctga aatgctcgag
     6181 gccgcgcgcg gttttcttac ctcccgcgga gtcgcgaacg tcgagtttcg cgtggcggac
     6241 gccaccagcc ttcccttcac ggacggcgag ttcgacgccg tcacctgccg gatcgcccct
     6301 caccactttc ccgatgtccg cgcgttcctg cgcgaggtct atcgcgtgct caagccgggt
     6361 ggggtcgcca tcgtggtgga caacgtcgtt cccgaggacg agagtgcggc ggcgttcatc
     6421 aacgagttcg agaagacgcg cgatccgagc cacgtctggg cgtatccgcc gtcaggctgg
     6481 gtgaggttct tcgaggaggc aggattcctc gacctgcagg tcgagtcctt cttcaaggcg
     6541 ctcgacttcg aggcgtggac cgatctcaag gacatggacc cagcgacgaa gagccggctc
     6601 cgcgacatga tcgtggaagc gccgcccaag gctctcgacg cgctcttccc ggaggaccgc
     6661 gatggcgcgc tctggttcta cctgcaagag ctactgatcc ggggtgtgaa gccggggagc
     6721 tgagcgcggc cgcagctcag accttccgga cggccgcgac ctcgagaatc cgctgaccct
     6781 tccgttccat cagactgccg cggatctcca cgtgctccga gaccatcccc atgcacttct
     6841 tgaagggatc ggggacaccc tggaacgaaa gtggcgccat ggcgcggagg tgatcgatcg
     6901 aaagcatgta gagatcaccc tgctccgtga gaatccccat gcagacgtcg ccggccacgc
     6961 acttgaggcc gcagtccttg tgctgggggc cggtgagccc cttctcgatg tagcagtagt
     7021 aatccacgat ctcgccgcgc acggtcacgg cgtgcttgcc gctgcccggc gggcaaacgt
     7081 ccccggtcgc ggggagtttc gtcgcgctcg acgtcttggg tgtggctccc gcgcccgcga
     7141 tggccgggtc gactccgcct cggagtgcgg cgccggcaat gccaagggcg acgtagacga
     7201 ggacgacgaa gcccgcgacc aggtgacgtt tcatggggag atcgtatcgc ccgccgccgc
     7261 gggcggggca ctatacggag gtattagtgg ggatggtccc ctggtggcga accgatccgt
     7321 ccatccgatt gacgttcctg agatggtgcg cttagatctt cgtctggttt ggacggtttt
     7381 cttctgtaaa tgtgacacct ctcgggcgat atgcacactc gcactgcttg gaagccgatc
     7441 ttcacaggca aggctgctga ggaagccagg ctatcgattc tcgcggtcgc tgaggaccta
     7501 cgggatcccc aatggtcccc ggagcgcctt cattacagct tggcgggggg cagtgcgggc
     7561 atcgccatct actatcacga gcttgcccaa gcatggcctc attccgactt ctcggaactt
     7621 cgggatcgtt attgggatgg agccctggac ggtctggctc aggctcccag cgcgcccaat
     7681 ctatacagcg gttacacagg tgtcggctgg gcctcgacct tcctagactc gtccgacact
     7741 ggccaggaga gcgaagcgga tgacgatgtt catgaagaac tcgaggaagc ccttctcgag
     7801 caactcgcaa tgattcagga tccagtcatc tacgacctga tcaatgg
//
LOCUS       DASTKC010000132         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1046660, whole genome shotgun sequence.
ACCESSION   DASTKC010000132 DASTKC010000000
VERSION     DASTKC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046660"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /gene="thpR"
                     /locus_tag="VFR25_05415"
     CDS             complement(<1..407)
                     /gene="thpR"
                     /locus_tag="VFR25_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET9940520.1"
                     /translation="MKDLTRTFVALLLPEDWMAYLAGVSRELAAGTSGCSWVRPENFH
                     LTVRFLGDLGDSGVRRVCDTMTEAASPLAAPRARLGELGGFPNLERPRVLWVGFAEGG
                     RELEAVGKSVNDALRRAGFGPPDKPFRPHLTLAR"
     gene            complement(404..1648)
                     /locus_tag="VFR25_05420"
     CDS             complement(404..1648)
                     /locus_tag="VFR25_05420"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET9940521.1"
                     /translation="MSAEIITIGTELLNGIGRDTNGAMIAAHLASIGIEVQYQTTVGD
                     DASRMADVFRAAAHRAQVVITTGGLGATSDDLTRKTIATVFRRRLVLDEAVLEHIRAR
                     FRVRGIEMPTINEAQALVPRGARIIENPRGLAPGLHFTHLEADYFSLPGVPHEAEAMM
                     ESYVIPYLKARRPGIPATRRVIRTVGISESVLAERLRPVEASEERIRVGYLPHIHGVD
                     ITLAPIAPELAWMEDVLDRCERRVRELAGDHAYGAEGDTLSAVVGALLLERRATLATA
                     ESFTAGAVGSAITATPGASRYYLGGIVAYSNRAKQELLGVRATTLERHGAVSAETAEE
                     MASGARTRFDCDVALSSTGIAGPDGGSTEKPVGLVYFGIATSDGVRSARFVLPGNRAE
                     ITGRSVAYLLDLARRQLAPAAV"
     gene            complement(1645..>2171)
                     /locus_tag="VFR25_05425"
     CDS             complement(1645..>2171)
                     /locus_tag="VFR25_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET9940522.1"
                     /translation="RGVRHPQARVQDGPEDARLTSVRRIAIAIATGLGVGFAPVAPAT
                     VASFLVALLLLPAVGHVSTLAWGLGTLALFPIAVWSSHQAEKTLGTDAKPIVIDEVMG
                     MLVGGFAVPMGSRPIVALALLFVFFRVFDIWKPLGIHRSQSLPGGWGVVVDDVLAGVL
                     TNVALRVAVGWVSP"
BASE COUNT          349 a          771 c          762 g          289 t
ORIGIN      
        1 cgggccagcg tgaggtgcgg gcggaacggc ttgtcggggg gaccgaaccc ggccctgcgg
       61 agcgcatcgt tcacgctctt cccgaccgcc tcgagctcgc gtcctccctc cgcgaacccg
      121 acccagagga cccgtgggcg ctccagattc gggaagccgc ccagctcccc gagtctcgcc
      181 cgcggcgctg cgagcggcga cgcggcctcg gtcatcgtgt cgcagacgcg gcgcaccccg
      241 gagtcgccga ggtcgccgag gaagcggacc gtcaggtgga agttctcggg ccgcacccac
      301 gagcatcccg aggtccccgc cgcgagctcg cgcgagaccc ccgccaggta ggccatccag
      361 tcttcgggaa ggaggagcgc gacgaaggtg cgggtgaggt ccttcacacg gcggccggcg
      421 cgagttgccg ccgggcgagg tcgaggagat acgccaccga ccgccccgtg atctcggcgc
      481 gattccccgg aaggacgaag cgcgcgctcc ggacgccgtc ggaggtggcg atgccgaagt
      541 agacgagacc cacgggcttc tccgtgctcc cgccatccgg gccggcgatg cccgtgctcg
      601 agagcgcgac gtcgcagtcg aagcgcgtcc gcgcgccgga ggccatctcc tcggcggtct
      661 cggcgctcac cgcgccgtgc cgctcgagcg tggtcgcgcg aacgccgagg agctcctgct
      721 tcgcgcggtt cgagtaggcc acgatcccac ccaggtagta gcgcgacgcg ccgggggtcg
      781 ccgtgatcgc ggatcccacc gcccccgcgg tgaacgactc cgcagtcgcg agcgtggcgc
      841 ggcgctcgag gaggagcgcc ccgaccacgg cggagagcgt gtccccctcc gccccatagg
      901 catgatcacc cgccagctcg cggacgcggc gctcgcaacg gtcgagcacg tcctccatcc
      961 aggcgagctc gggagcgatc ggcgccagcg tgatgtcgac gccatgaatg tgcggcagat
     1021 agccgactcg gatccgctcc tccgacgctt ccaccggacg gagccgctcc gcgagcaccg
     1081 attccgagat cccgaccgtg cggatgacgc gtcgagtcgc cggaatgccc ggccgccgcg
     1141 ccttgagata cgggatgacg tagctctcca tcatcgcctc ggcctcgtgc ggcacgccgg
     1201 ggagcgagaa gtagtcggct tcgaggtggg tgaagtggag gccgggggcc aggccgcggg
     1261 gattctcgat gatgcgcgcc ccccgtggaa cgagcgcctg cgcctcgttg atggtgggca
     1321 tctcgatccc gcgcacgcgg aatcgcgccc ggatgtgctc gagcacggcc tcgtcgagca
     1381 cgaggcggcg ccggaacacg gtcgcgatcg ttttccgcgt gaggtcgtcc gatgtggcgc
     1441 cgagacctcc ggtcgtgatc acgacctgag cgcggtgcgc ggcggcgcga aagacgtccg
     1501 ccatgcgcga ggcgtcgtcc cccaccgtcg tttggtactg cacctcgatc ccgatcgacg
     1561 cgagatgggc cgcgatcatg gccccgttcg tgtcccgtcc gatcccgttc aggagctcgg
     1621 tgccgatcgt gatgatctca gcgctcatgg cgagacccat cctaccgcaa cccgcagcgc
     1681 gacgttcgtg agcacgcccg cgagcacgtc gtccacgacg acgccccagc cgcccgggag
     1741 cgactgcgag cggtggatgc cgagcggctt ccagatgtcg aagacgcgga agaaaacgaa
     1801 gaggagcgcc agagcgacga tcggccgcga ccccatcggc accgcgaagc cgccgacgag
     1861 catccccatc acctcgtcga tcacgatcgg cttcgcgtcc gtgccgagcg tcttctcggc
     1921 ctgatgcgac gaccagaccg cgatcggaaa gagcgcgagt gtcccgagac cccatgcgag
     1981 cgtgctcacg tggccgacgg ccggtaacaa aagaagggcg acgaggaagg aggcgacggt
     2041 ggccggcgcg acaggggcaa agcccacgcc gagccccgtg gcgatcgcga tcgcgatccg
     2101 gcgcacggat gtcagccgag cgtcttcagg accatcttgc acgcgagctt gagggtgtcg
     2161 aacaccccgg t
//
LOCUS       DASTKC010000133         6587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_811807, whole genome shotgun sequence.
ACCESSION   DASTKC010000133 DASTKC010000000
VERSION     DASTKC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811807"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1312)
                     /gene="hemL"
                     /locus_tag="VFR25_05430"
     CDS             complement(8..1312)
                     /gene="hemL"
                     /locus_tag="VFR25_05430"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET9940523.1"
                     /translation="MNETRPAVTTKKSEALHRRALELFPGGVNSPVRAFQAVGGVPRQ
                     IVAAHGAYLTDADGQELLDYVLGWGPILLGHAHPAVIQAIERQARLGTLYGASTPLEA
                     DLAERVRRFYPAAERLRFVSTGTEATMSALRVARAATKRSLYVKLEGCYHGHADPFLI
                     QAGSGAATLGVPDSPGVPASALADTRLARFNDLDSVRAHFDREGSAIAAVIVEPVVGN
                     YGVIPPRPDFLPGLRALCDRHQAILIFDEVMCGFRAARGGAAERYGVKPDLVTLGKVI
                     GGGLPVAAFGGRSDLMALVAPEGPVYQAGTYSGNPLSMAAGQSALAEMEKDPDFFRKV
                     EARTSLLAAGLKASLDRHRVPGVVNAVGSMWTLFFGVTEVSTMDQVRRADRERYARWF
                     QGMLERGIYLPPSAFEAAFLSAAHTETEIDRTLEAADEVLAS"
     gene            complement(1309..2295)
                     /gene="hemB"
                     /locus_tag="VFR25_05435"
     CDS             complement(1309..2295)
                     /gene="hemB"
                     /locus_tag="VFR25_05435"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET9940524.1"
                     /translation="MTYPTTRLRRLRATPALRAMRRETTLAASNLVAPLFVCHGSAVR
                     KPVASMPGHFQLSVDELVREGEAIARDGIGGVILFGLPAAKDPEGREAYDEKGIVPTA
                     LRALKKAVPDLILWADVCLCEYTSHGHCGVLNGDKVDNDKTLPLLARAATAYAAAGAD
                     LIAPSDMMDGRVAAIRKALDMGGFPDTPIVSYAAKYASAFYGPFREAAESTPQFGDRR
                     GYQMDPANAAEALREVALDLEEGADMVMVKPALPYLDVIRRVKEQFQVPVAAYNVSGE
                     FAMIKAAAQNGWIDGERAALETLVSIRRAGADVILTYFAREIAPLLAKGATA"
     gene            complement(2292..3053)
                     /locus_tag="VFR25_05440"
     CDS             complement(2292..3053)
                     /locus_tag="VFR25_05440"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET9940525.1"
                     /translation="MSALLVVARMDPRDDKIVEAAEACSFRVARLPLLATEPGRDVTR
                     FLTWLERAPEGTAIAWTSRRAAEALARTALPAHEAALAGLPLFAVGEESAAPILGRHL
                     DVEYVTQEPSARSLGRQIADARASRGIHRVAFLRGDRSLPDLPDELERAGIEVEPFEL
                     YRTTFQDVDVDVVRSAIAANDPIVVLYFSPSGIEALERLLPDVLNLLRSRAHAVPIGD
                     TTRRALTTRSYATIAEPGVDPFAGAALQSVKRIPR"
     gene            complement(3050..3970)
                     /gene="hemC"
                     /locus_tag="VFR25_05445"
     CDS             complement(3050..3970)
                     /gene="hemC"
                     /locus_tag="VFR25_05445"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9940526.1"
                     /translation="MRIGTRGSALALAQSGLVQKLLLGGEGRNILTVIETTGDRNPLA
                     SLRRIGGKGIFTKEIDTALLDRRIDLAVHSLKDLPTEETPGLAIGALLTREDPRDALV
                     SRGGVGLDQLPQGAVVGTSSFRRRAQLLARRPDLTVEDLRGNVPTRIARVLSGTVDAI
                     VIAAAGLIRLGMESEIAEILDESVMLPAPGQGIIAVQIRADDAETREQLRAIHDPAAE
                     AEATAERTFLSALGGGCLVPVGARAVARDGRLALSGFVGHPEGRPSIRKSIEGPTAGA
                     AELGAELARHALAQGAREILDAVRSDEVFP"
     gene            complement(3948..5270)
                     /gene="hemA"
                     /locus_tag="VFR25_05450"
     CDS             complement(3948..5270)
                     /gene="hemA"
                     /locus_tag="VFR25_05450"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9940527.1"
                     /translation="MSAAAETMAPELGFFCVGLNHETSPLEIRDALVLSDEEVARAIR
                     ALRERAGASEALVISTCNRTEVYARGAELPNPPAFVAALLQEIKGLDLAGSGKSYLYT
                     YREPESIRHLFRVACGLDSQILGEPQITGQVKDSLTLAGKLGGSGPVMERLLDAALRC
                     SKRARTETGIGRGPVSSAYAAVNLATKVLGSLADKKVLLIGAGEMAGLAACHFMEAGI
                     AQFMVANRSRERAEKLSATIAARVISLEAIQVALPGADIVVSATSAQEPIVSEAMVRN
                     AMKIRKNRPLLFLDLAVPRDVDPHVAKLPNVFVHDLDALGQLVAQSLAQRRAEVPKVE
                     AIIESEMARFLRWHRSLAVKPTVTAFRSHFEEIAKEELDRHRGRFRPEDHAALEALVH
                     GIVQKLLHRPTTRLSRADDEGGGIARVDAVRDLFGIDQEDPDADRNAR"
     gene            complement(5267..6079)
                     /gene="ccsA"
                     /locus_tag="VFR25_05455"
     CDS             complement(5267..6079)
                     /gene="ccsA"
                     /locus_tag="VFR25_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9940528.1"
                     /translation="MIPLLTTTLPVLYALSVAAYGRAYADEASTGGRWGPVALRLAVL
                     VHVLYLSARGVTEGHLPLASAYDFLSATALSLAAVYIYLELRQGVRTTGIFVLPLVFL
                     MQVISSAYGNTIPARQPPLFPFWFELHTMTAVLGYGAFFVSAIYGVLFLLLYREIKAN
                     RLSFFFRRMPPLETLGRMNVSAASAGLVLLGLAILMGIGWARMANVALARDPKTWLTV
                     GAWLLLGFAVLAYHRLGWRGPRAVYASLAGFTTLLLSRVVTDLFVRSFHTWR"
     gene            6278..>6587
                     /locus_tag="VFR25_05460"
     CDS             6278..>6587
                     /locus_tag="VFR25_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9940529.1"
                     /translation="MPRAGHAHAIERIALDVIQCERCPRLREYCLEVARVKRAAYRDE
                     TYWGKPVPGFGDPKAWLLIVGLAPGAHGANRTGRMFTGDKSGEWLYGELHRQGLANRP
                     D"
BASE COUNT         1006 a         2280 c         2359 g          942 t
ORIGIN      
        1 ccctgcgtca cgacgcgagc acctcgtccg ccgcctcgag cgtgcgatcg atctcggtct
       61 cggtgtgcgc ggccgagagg aaggccgcct cgaacgcgga cggcgggagg tagatgccgc
      121 gctcgagcat tccctggaac catcgggcgt agcgctcgcg gtcggcgcgg cgcacctgat
      181 ccatcgtcga gacctcggtc acgccgaaga agagcgtcca catcgagccg accgcgttga
      241 ccacgcccgg gacgcgatgg cggtcgagcg atgccttgag tcccgcggcg aggagcgacg
      301 tgcgcgcctc caccttgcgg aagaaatcgg gatccttctc catctcggcg agcgcggact
      361 ggccggccgc catggagagc ggattccccg agtacgtgcc ggcctggtag accgggcctt
      421 cgggagcgac gagcgccatg aggtcggagc gcccgccgaa cgccgcgacg ggcaatccac
      481 cgccgatcac cttgccgagc gtgacgaggt cgggcttcac gccgtaccgc tccgccgctc
      541 cgccgcgcgc cgcgcgaaag ccgcacatca cctcgtcgaa gatgaggatc gcctgatgcc
      601 gatcgcacag ggcgcggaga cccgggagga agtcgggccg gggcgggatg acgccgtagt
      661 tcccgaccac gggctccacg atgaccgcag caatcgcgct tccctcgcgg tcgaagtggg
      721 cgcgcacgga atcgagatcg ttgaaccgcg cgagccgcgt gtcggcgagg gcggacgcgg
      781 ggacgccggg cgaatcgggc acgccgagcg tcgccgcgcc cgagccggcc tggatcagga
      841 acggatcggc atggccgtgg tagcagccct cgagcttcac gtagagcgac cgcttcgtcg
      901 cggcgcgcgc cacgcggagc gcgctcatgg tcgcttcggt tcccgtcgac acgaaacgga
      961 ggcgctcggc ggccggatag aagcggcgca cgcgctcggc caggtccgct tcgagcggcg
     1021 tgctcgcgcc gtagagcgtc ccgagccgcg cttgccgctc gatggcctgg atgacggcag
     1081 gatgcgcgtg tccgaggagg atcggccccc acccgagcac gtagtcgagc agctcctggc
     1141 cgtccgcgtc cgtgagatag gcgccgtgcg ccgcgacgat ctgacgcggc acgccgccga
     1201 ccgcctggaa cgcgcgcacg ggagagttca cgccgccggg gaagagctcg agcgcgcggc
     1261 gatgcagcgc ctcgctcttc ttggtggtca cggcagggcg ggtctcgttc acgccgtcgc
     1321 ccccttcgcg agcaggggag cgatctcccg cgcgaagtag gtcaggatca cgtcggcccc
     1381 cgcgcgccgg atcgagacga gggtttcgag cgcggcccgc tcgccgtcga tccatccgtt
     1441 ctgcgccgcg gccttgatca tcgcgaactc gcccgacacg ttgtacgccg cgaccggcac
     1501 ctggaactgc tccttcaccc ggcggatgac gtcgaggtag gggagcgcgg gtttcaccat
     1561 caccatgtcc gccccttcct cgagatcgag cgccacctcg cgcagggctt ccgcggcatt
     1621 cgcgggatcc atctggtagc cgcgccggtc gccgaactgg ggggtggact cggccgcctc
     1681 gcggaatggg ccatagaacg cgctcgcgta cttggcggcg taggagacga tcggcgtgtc
     1741 gggaaagccg cccatatcga gcgccttccg gatcgcggcg acgcggccgt ccatcatgtc
     1801 gctcggcgcg atgagatcgg cgcctgccgc ggcataggcg gtggcggcgc gggccaggag
     1861 cgggagcgtc ttgtcgttgt cgaccttgtc gccgttcagg acgccgcagt gcccgtggct
     1921 cgtgtactcg cagagacaga cgtccgccca gaggatgaga tccgggaccg ccttcttgag
     1981 ggcgcggagc gcggtcggaa cgatgccctt ctcgtcgtac gcctcgcggc cttccgggtc
     2041 cttggcggcg gggagtccga agaggatgac gccgccgatg ccgtcccgcg caatcgcctc
     2101 gccttcgcgg acgagctcgt cgacggagag ctggaagtgg ccgggcatgg acgcgacggg
     2161 cttcctcacg gccgatccgt gacagacgaa gaggggcgcg acgaggttcg acgcggcgag
     2221 cgtcgtctcg cggcgcatgg cgcgaagcgc cggagtggcg cggagcctgc gaaggcgtgt
     2281 ggtcgggtag gtcaccgagg aatcctcttc accgattgta aagccgcccc tgcgaacggg
     2341 tcgacgccgg gctcggcgat cgtcgcgtag cttcgggtcg tgagcgcgcg ccgcgtggtg
     2401 tcgccgatcg gcaccgcgtg cgcgcgggag cggagcaggt tcagcacgtc ggggaggagc
     2461 cgctcgagcg cctcgatccc cgacgggctg aagtagagga cgacgatcgg gtcgttggcc
     2521 gcgatcgcgg agcgcaccac gtcgacgtcg acgtcctgga acgtcgtgcg gtagagctcg
     2581 aacggctcga cctcgatccc ggcgcgttcg agctcgtcgg ggagatcggg cagcgaacga
     2641 tcgccgcgca ggaacgcgac ccggtggatg ccccgcgatg cgcgcgcatc cgcgatctgc
     2701 cggccgaggc tcctggccga gggctcctgc gtgacgtact cgacgtcgag atgccgcccg
     2761 aggatcggcg ccgcgctctc ctcaccgacc gcgaagagcg gaagtcccgc gagcgctgct
     2821 tcatgcgcgg gtagcgcggt cctggcgagc gcctccgccg cccggcggct ggtccatgcg
     2881 atggccgttc cctcgggcgc ccgctcgagc cacgtcagga accgcgtcac gtcccgcccc
     2941 ggctcggtcg cgaggagcgg gaggcgcgca acgcggaacg agcacgcctc ggcagcctcg
     3001 acgatcttgt cgtcgcgcgg atccatgcgt gccacgacga ggagcgcgct cacgggaaga
     3061 cctcgtcgct tcgcaccgcg tccaggatct cgcgcgcccc ctgggcgagc gcgtggcgcg
     3121 cgagctccgc cccgagctcg gcagctccgg cggtcggtcc ctcgatcgac ttccggatcg
     3181 aagggcgacc ctccggatgg ccgacgaagc ccgatagcgc gagccttccg tccctcgcga
     3241 ccgcgcgcgc gccgaccggc acgaggcatc cgccgccgag tgcggacagg aacgtgcgct
     3301 ccgccgtggc ttcggcttcg gctgccgggt cgtggatggc gcggagctgc tcgcgcgtct
     3361 cggcgtcatc ggctcggatc tggaccgcga tgatcccctg gcccggggcg gggagcatca
     3421 ccgactcgtc caggatctcc gcgatctcgc tctccattcc gaggcggatg aggccggccg
     3481 cggcgatcac gatggcgtcg accgtgcccg agaggacgcg cgcgatgcgc gtgggcacgt
     3541 tgccgcgcag atcctccacc gtgaggtcgg gcctccgcgc gagcagctgc gcgcgccggc
     3601 ggaacgacga ggtcccgacg accgcgccct gggggagctg gtccagcccc acgccgccgc
     3661 gggacacgag cgcgtccctc ggatcctcgc gcgtcaagag cgccccgatc gcgagccccg
     3721 gcgtctcctc ggtcggcaga tccttgagcg agtgcaccgc gaggtcgatg cgccggtcga
     3781 ggagcgccgt atcgatctcc ttcgtgaaga tccccttgcc gccgatgcgc ctcagcgagg
     3841 cgagcggatt tcggtccccg gtcgtctcga tgacggtcag gatgttccga ccctccccgc
     3901 ccaggaggag cttctgcacg agcccgctct gcgcgagtgc gagcgcgcta ccgcgcgttc
     3961 cgatccgcat ccggatcctc ctggtcgatt ccaaagagat cccgcaccgc gtcgacacgg
     4021 gcgatgcctc cgccctcgtc gtccgcgcgg gagagccgcg tggtggggcg atggagtagc
     4081 ttctgcacga tgccgtgcac gagcgcctcg agcgccgcgt ggtcctccgg acggaaccgg
     4141 ccgcgatggc ggtccagctc ctccttcgcg atctcctcga agtggctccg gaacgcggtc
     4201 accgtcggct tgaccgcaag cgaccggtgc catcggagga agcgcgccat ctccgactcg
     4261 atgatcgcct ccaccttggg cacctcggcg cgccgctgcg cgaggctctg cgcgacgagc
     4321 tggccgagcg cgtccagatc gtgcacgaac acgttcggga gcttggccac gtgcggatcc
     4381 acgtcccggg gcaccgcgag atcgaggaag aggagcggcc gattcttccg gatcttcatc
     4441 gcgttccgga ccatcgcctc gctcacgatc ggctcctggg ccgacgtcgc gctcaccacg
     4501 atatccgcgc ccgggagcgc gacctggatc gcttcgagcg agatcacgcg cgcggcgatc
     4561 gtggcgctga gcttctcggc gcgctcgcgg ctccggttcg cgaccatgaa ctgggcaatg
     4621 ccagcctcca tgaagtggca ggcggcgagc cccgccatct cccccgcacc gatcaggagc
     4681 accttcttgt ccgcgaggga cccaaggacc ttggtcgcga gattcaccgc ggcgtacgcg
     4741 ctcgacaccg gcccgcgtcc aatgcctgtc tcggtccgcg cgcgcttgga gcagcggagc
     4801 gccgcgtcga ggagccgctc catgaccggc cccgagccgc cgagcttgcc ggcgagcgtg
     4861 agggagtcct tcacctgtcc cgtgatctgc ggctcgccga ggatctgcga gtcgaggccg
     4921 cacgccacgc ggaagagatg ccggatcgat tccggctcgc ggtaggtgta gaggtagctc
     4981 ttgcccgagc cggccaggtc gaggcccttg atctcctgga ggagcgccgc cacgaaggcc
     5041 ggcgggttgg gaagctccgc gccgcgcgcg tagacctcgg tccggttgca ggtcgagatg
     5101 acgagcgcct cgctcgcccc ggcgcgctcc cggagcgccc ggatcgcgcg cgccacctcc
     5161 tcatcggaga ggacgagcgc gtcccggatc tcgagcggcg acgtctcgtg gttcagaccg
     5221 acgcagaaga agccgagctc cggcgccatc gtctccgcgg ccgcgctcat cgccacgtgt
     5281 ggaacgatct cacgaagaga tccgtcacca ccctcgaaag aaggagcgtc gtgaatccgg
     5341 cgagcgacgc gtagaccgcg cgcggccctc gccagccgag acggtgatac gcgagcaccg
     5401 cgaatccgag cagcagccag gcgccgaccg tgagccaggt cttgggatcc ctcgcgagcg
     5461 cgacgttcgc catccgcgcc catccgatcc ccatgagtat ggcgaggccg aggagcacga
     5521 gccccgccga cgccgcgctc acgttcatcc gcccgagcgt ctcgaggggg ggcatgcgcc
     5581 gaaagaagaa gctgagccgg ttcgccttga tctcccggta gaggaggagg aacaggacac
     5641 cgtagatggc cgagacgaag aaggccccgt atcccagcac ggcggtcatc gtgtggagct
     5701 cgaaccagaa cgggaagagc ggcggctggc gcgcggggat cgtgttcccg taggccgacg
     5761 agatcacctg catgaggaag acgagcggga ggacgaagat tccggtcgtg cgaaccccct
     5821 ggcgaagctc gaggtagatg tagaccgcgg cgagcgacag cgccgtcgcg gagaggaagt
     5881 cgtacgccga ggcgagcggc aggtgcccct cggtcacgcc ccgcgccgag agatagagca
     5941 cgtgcacgag caccgcgagc cggagcgcca cggggcccca gcggccgccg gtcgaggcct
     6001 cgtcggcata ggccctcccg tacgccgcca cgctcaaggc gtagaggacg ggcagggtcg
     6061 tcgttaagag cggaatcaag ggttgcatcg gatgggttca agcctcccca gactgagcct
     6121 cattgtcccg ccaaacgctc gattttacaa ggacctgggg cggagccggt ggggtcatgg
     6181 gcacctctac cttagtaccc gcaaagggtg tcgaggttgc cgctggggga ctggcgcccg
     6241 ggcggaggtg cggggccggg gcgctatagt ggctttgatg ccacgtgccg gccatgctca
     6301 tgcgatcgaa cggatcgcgc tcgacgtcat ccagtgcgag cgctgcccgc gtctccggga
     6361 gtactgcctc gaggtcgcgc gcgtgaagcg ggccgcctat cgcgacgaaa cctactgggg
     6421 aaagcccgta ccgggcttcg gcgatcccaa ggcctggctc ctgatcgtgg ggctcgcgcc
     6481 cggcgcgcat ggcgcgaacc gcacggggcg gatgttcacg ggggacaagt cgggggagtg
     6541 gctctatggc gagctccatc ggcagggtct cgcgaatcgc cccgatt
//
LOCUS       DASTKC010000134         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1521184, whole genome shotgun sequence.
ACCESSION   DASTKC010000134 DASTKC010000000
VERSION     DASTKC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521184"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFR25_05465"
     CDS             complement(<1..353)
                     /locus_tag="VFR25_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940530.1"
                     /translation="MLPELDALLALQRQDSLLLEARRKRDDIPRRKEALRLQVNQAKA
                     ELEQAKKDLEQARIVRRAAEKDVEGLTADSVKLERQLHDVKTNKEYQAILHEIEGIKS
                     KRSDYETRILESFER"
     gene            complement(461..536)
                     /locus_tag="VFR25_05470"
     tRNA            complement(461..536)
                     /locus_tag="VFR25_05470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Val,seq:cac)
     gene            complement(604..677)
                     /locus_tag="VFR25_05475"
     tRNA            complement(604..677)
                     /locus_tag="VFR25_05475"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(641..643),aa:Ile,seq:cat)
     gene            complement(762..2507)
                     /gene="rpoD"
                     /locus_tag="VFR25_05480"
     CDS             complement(762..2507)
                     /gene="rpoD"
                     /locus_tag="VFR25_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET9940531.1"
                     /translation="MKREKTDRFHDLKVLAGKQQYLLHDQIETFLDESASVDDMDEVY
                     IALNDLKVDVFDTHEEAQQKLKIKRKDEKKPAEKQVLPHPMRYDDPVRMYLREMGRVP
                     LLDREGEVEIAKRIKAGERQICTALFRSSPILKELIGLGTKLKQGKVRVEDLTQIDFG
                     NWNPEFSPKRERARVLKSIDRIRVLHGRLAKVQDRSVGRLSEEKRRAHMEKVRHADEE
                     LLRELYTLNINNKLIDRLAERMKGFSKKVEETETNLTRMEWKWGKSGDEVHGLIKRLK
                     KSRKEAKAIEKETGIRVADLEAYELEYKNCRRLVKKIEVEAKMTRDQIREVATEIRSG
                     EIARDKAKREMIEANVRLVISIAKRYTNRGLEFLDLIQEGNSGLMRAVDKFDYRKGYK
                     FSTYATWWIRQAITRAIADQARTIRVPVHMIEAINKVVRTSRRLVQELGREPTPEEVA
                     DKLQLPVDKIKSVLKAAQEPISLDRPIGEDDDSNLGDFIEDTSVVSPAQAAAFSMLKD
                     EVNEVLETLSKREAKVIRLRFGLTEDGCPRTLEEVGATFNVTRERIRQIEAKALRKLR
                     HPTRSRRLKGYVEMM"
     gene            complement(2550..>3538)
                     /locus_tag="VFR25_05485"
     CDS             complement(2550..>3538)
                     /locus_tag="VFR25_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET9940532.1"
                     /translation="QARALARFDAAAILVYDGDDAGIRAADRALEPMLAAGLQVSILL
                     LPEGEDPDSYLLQHGAQAFAGLVEGAMDPAAFLATARLSAPGANPTQEARVKRYVELL
                     GRVEDPVRRRLMVRRGAVAFGVEEDVLLEALGRKKGVGRRPMAPRAAVPTRAVPAGEP
                     AAGPTPRSEGTEVPEVIDPIERELAARCLTEDGAVVEVALSGGVSCFRSKNLRALLSD
                     WLTMGRAPLPEELKALESLDSFARSLLAEHPVEEGTSDEAARKTARELLQRLGERRIK
                     ASLAELDRAIRQAERSREGDLDRLVAERRDLASKLHSGNWLEDQRRNHPAAS"
BASE COUNT          493 a         1224 c         1119 g          702 t
ORIGIN      
        1 tcgcgctcga aggactcgag gatccgcgtc tcgtagtcgg agcgcttgga cttgatccct
       61 tcgatctcgt ggaggatcgc ctggtattcc ttgttcgtct tcacgtcgtg gagttgccgc
      121 tcgagcttca cggagtcggc cgtgagccct tccacgtcct tctccgccgc gcggcgcacg
      181 atccgcgcct gctcgagatc cttcttggcc tgctccagct cggccttcgc ctgattcacc
      241 tggagccgca gcgcctcctt gcgtctcggg atgtcatcgc gtttccttcg cgcctccagg
      301 aggagcgaat cctgccgttg cagcgcgagc agcgcgtcca attcagggag caacgttcac
      361 ctccaaagaa aaacggggag cgcgttcatc cgcgctcccc gtgcggggtc gtggggcccg
      421 gtttcggccg agccccatcg aagggtcggt cccgggtcca tggtgggcgg tgcagggatt
      481 gaacctgcga cctctggctt gtgataccag cgctctccca actgagctaa ccgcccccga
      541 ggcatgttgg atggcggagc cgcgtggctc cgcgcacgac gtcgtggtga tgattgcggc
      601 acatgggccc acttggactc gaaccaagga ccgtccggtt atgagccgga tgctctcacc
      661 agctgagcta tgggcccgaa tgcccttctt ggttcgtctc gaggacctga gccgcgcagc
      721 gtagcatcgc ccgtccgcga gggtcaagac gcggcggtcg atcacatcat ctccacgtag
      781 cccttgaggc ggcggctgcg cgtgggatgc cgcagcttgc ggagcgcctt cgcctcgatc
      841 tggcgaattc gctcgcgcgt gacgttgaac gtggcgccga cctcttcgag cgtgcgcggg
      901 cagccgtcct cggtcaaacc gaaccgcaga cgtatcacct tcgcctcgcg cttgctcaga
      961 gtttcgagca cctcgttcac ctcgtccttc agcatcgaaa atgccgcggc ctgagccgga
     1021 gagaccacgc tcgtatcctc gatgaaatcg ccgaggttcg agtcgtcgtc ctcgccgatc
     1081 ggtcgatcca gcgagatcgg ctcctgcgcg gccttgagca cgctcttgat cttgtccacc
     1141 gggagctgca gcttgtcggc cacctcttcg ggggtcggct cccggcccag ctcctgcacc
     1201 aggcggcgcg acgtgcgcac gactttgttg atcgcctcga tcatgtgcac cgggacgcgg
     1261 atggtccgcg cctggtcggc gatcgcgcgc gtgatggcct gacggatcca ccaggtcgca
     1321 taggtcgaga acttgtagcc cttccggtaa tcgaacttgt cgaccgcgcg catgagcccc
     1381 gagttccctt cctggatcaa gtcgaggaac tcgagtccgc ggttcgtgta gcgcttcgcg
     1441 atggagatca cgaggcggac gttcgcctcg atcatctccc gcttcgcctt gtcgcgcgcg
     1501 atctcgccgg agcggatctc ggtcgccacc tcgcggatct ggtcgcgggt catcttcgcc
     1561 tcgacctcga tcttcttcac caggcggcgg cagttcttgt actcgagctc gtacgcctcg
     1621 aggtcggcga cccggatgcc ggtctccttc tcgatcgcct tggcttcctt gcggctcttc
     1681 ttgaggcgct tgatgagacc gtggacctcg tcgcccgact tgccccactt ccactccatg
     1741 cgggtgaggt tggtctcggt ctcctccacc ttcttcgaga agcccttcat ccgctcggcg
     1801 agccggtcga tgagcttgtt gttgatgttg agcgtgtaga gctccctcaa gagctcctcg
     1861 tccgcgtggc gcaccttctc catgtgcgcg cggcgcttct cctcggagag gcgccccacg
     1921 gagcggtcct gcaccttcgc gagccgcccg tgcagcacgc ggatccgatc gatcgatttg
     1981 agcacgcggg cacgctcgcg cttgggcgag aactcgggat tccagttgcc gaaatcgatc
     2041 tgggtgaggt cctcgacccg caccttcccc tgcttgagct tggtgccgag gccgatcagc
     2101 tctttcaaga tcgggctcga gcggaagagc gccgtgcaga tctggcgctc gcccgccttg
     2161 atgcgcttcg cgatctcgac ttcgccctcg cggtcgagca gcggcacgcg tcccatctcg
     2221 cgcaggtaca tgcggaccgg atcgtcgtac cgcatcgggt gcgggaggac ctgcttctcg
     2281 gccggcttct tctcgtcctt gcgcttgatc ttgagcttct gctgggcttc ctcgtgggtg
     2341 tcgaagacgt cgaccttcag gtcgttcagc gcgatgtaga cctcgtccat gtcatcgacc
     2401 gaggcgctct cgtcgaggaa cgtctcgatc tgatcatgga gcaggtactg ctgcttcccg
     2461 gccaggacct tcaggtcgtg aaagcggtct gtcttttcac gcttcatccg gtgacgtctc
     2521 cttcgttcgt ggtgattccg cccgagccct tacgaagccg ccgggtggtt gcggcgttgg
     2581 tcttccagcc agtttccact gtgtaacttg gatgcgagat cccgccgttc ggccaccagc
     2641 cggtcgaggt ccccttcccg ggaccgctcc gcctgccgga tggcccggtc cagctcggcc
     2701 agcgaggcct tgattcgacg ctccccgagc cgctggagga gctcccgggc cgtcttccgg
     2761 gccgcctcgt ccgacgtccc ctcctcgacc gggtgctcgg cgaggagcga ccgggcgaag
     2821 ctgtccaggg actccaaggc cttgagctct tcgggcaacg gggcccgtcc catcgtgagc
     2881 cagtccgaga gcagagctcg taagttcttg ctccgaaagc agcttactcc accgctcagg
     2941 gcgacttcga cgacggcgcc gtcctcggtg aggcagcggg cagccagctc ccgctcgatc
     3001 ggatcgatga cctcggggac ctcggtccct tcggaacgcg gcgtcgggcc cgcggccggc
     3061 tcgcctgcgg ggaccgcccg tgtcgggact gccgcccggg gggccatggg ccgccggccg
     3121 acacccttct tacgccccaa agcctcgaga agcacgtctt cttcgacccc gaaggccacg
     3181 gcgccccggc ggaccatgag ccgccgccgc accggatcct ccacccgccc gagaagctcc
     3241 acgtaccgct ttacccgggc ttcctgggtc gggttcgcgc caggcgcgga aagccgcgcg
     3301 gtcgcgagga acgcggccgg atccatggcc ccctcgacaa gcccggcgaa ggcctgggcg
     3361 ccgtgctgga gcaggtacga gtcgggatcc tccccttccg gcagaagcaa aatgctcacc
     3421 tggagcccgg ccgcgagcat gggctcgagc gcgcggtccg cggcgcggat gccggcgtcg
     3481 tccccgtcgt agacgaggat cgccgcggcg tcgaaccgcg cgagcgcgcg cgcctggt
//
LOCUS       DASTKC010000135        23762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_813405, whole genome shotgun sequence.
ACCESSION   DASTKC010000135 DASTKC010000000
VERSION     DASTKC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 23762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23762
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813405"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VFR25_05490"
     CDS             complement(<1..1467)
                     /locus_tag="VFR25_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940533.1"
                     /translation="MAPEPVSPQGRPWAPARIALAGILALAIAHVFLFRGYLTDDTFI
                     HMQFAKNLLAGHGFSFNAGEPTYGATSPLWVLLIALTGLAVPGSSATPLDPAALPPLA
                     GIAKMWGLLSLLGAILLLYRLGRRLGWSSRQALLAPFFLAANAWVARWTGSGMETPLA
                     IFLVVASLDALARTVLENRSAWPAGILLGLAFLARPECVLLAFLAAVAVGANATTRKR
                     LVGLFGGALLAAGPWLLTAWAWFHRILPNTSAAKAGAFGDLELATSALRTSLRILLAT
                     DALPIGLAIVGLALAGPEALRKQSPERRAFWIAVSAWPLALLFGLAMGGVQVVSRYLL
                     PALPSIALLGAASMAWGGVYLRPPLRSLAWTALVLLYLAQSAFLTIRYNVPHAVRHTA
                     GLRSSLVAFGVWARHETPPGTLFALPDIGAFGYYADRPVLDLFGLVTPGMARVAVRAG
                     YDAVVDRLLFEPYGRPSYLVDRARTAGRLERKDDPDDPY"
     gene            complement(1449..3470)
                     /gene="tkt"
                     /locus_tag="VFR25_05495"
     CDS             complement(1449..3470)
                     /gene="tkt"
                     /locus_tag="VFR25_05495"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9940534.1"
                     /translation="MATIGAELDQLCVETIKFLSTDAVEKAKSGHPGAPMGGADMAFV
                     LWSKFLRFDPKDPHWLGRDRFVLSNGHASMLLYSLLHLFGFDLTLDDIRQFRQWGSRT
                     PGHPERGHAPGVEATTGPLGQGFGNGVGMALAHQMTLTRYPELEGALASRVFGIVSDG
                     DLMEGVASEAASLAGHLRLGNLIYLYDDNHISIEGSTGLAFTEDAAKRFDAYGWHTKR
                     IDGHEHRQIEEAIEEALQETKRPSLILARTLIGKGAPTKAGTAKVHGEPLGAEELKRA
                     KEAAGWPLTPEFYIPSDALERFRALSEEKRAAREEWNRRYDALRARAPEAARRFESAA
                     RLDLPEDLEARLLAAVDTKSSLATRAWSGKVIQAAARAVPWLVGGSADLSPSTITDID
                     QGGSVGREALEIEAPVDYSGRTLHFGVREHGMGAIVNGIALHGGYIPYGATFLVFSDY
                     MRPSIRLAALSKLRSIFVFTHDSVFLGEDGPTHQPIEQLPSLRLMPNLDVWRPADGPE
                     TAAAWASAIRRADGPTALMLTRQKLAPVGGSADLAARGGYVLREPSAGGTPVTLVASG
                     SETPLAVSAADLLAKKGIAARVVSMPCVEVFQRQAEDYRRNVLPPDGAFVVVEAAQTD
                     LWCSLVGADSLRIGLDHFGASAPAEVIAKEFGFTPNAVADRIERWLPSR"
     gene            3574..5046
                     /locus_tag="VFR25_05500"
     CDS             3574..5046
                     /locus_tag="VFR25_05500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9940535.1"
                     /translation="MIGNDIRSAGWSGLLLGVLVLGAAVGGCATNPATGAKQISLVSR
                     GQELDMGREADPAIISEYGLYDDPKLAAYVDSVGQRLGKVSHLPTLVWHFRLLDSPVV
                     NAFALPGGYIYITRGIVAQMNSEAQLAGVIGHEIGHVTARHSAQQITNQQIAQVGLLV
                     GMVAFSGFREYGGLAAQGMGLLFLKYSRDHETQADELGVQYTARAKYDPRQIPATYET
                     LKRIGDREGAGLPNFLSTHPDPGDRQVRTRQLAEATVGGAALNLEIGAANYRRHIEGL
                     VYGDDPRAGYFENSRFYHPQLGFQLIFPSGWKTVNNPSAVVATNESMGATMQLTLGST
                     KNESLTPSEYVDSLRVKNAITSASGHPEQFRDYPAWVGNVVMSGDQGGNVAAGFVRIK
                     PGQFLEIIGQSKGGAATEQIFAAIRSVAALTDPEKLNVTPDRIVIKPAPRTATFSELW
                     GEMGGGAIGVEDGAILNSTRATTTIQAGTPMKTVVRGKHA"
     gene            complement(5064..5588)
                     /locus_tag="VFR25_05505"
     CDS             complement(5064..5588)
                     /locus_tag="VFR25_05505"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9940536.1"
                     /translation="MAAGVLIEGSILLAPRIHRAEIEAWIRSAGVYGPLLLLAIQAGQ
                     ILAAPIPGVFVPVIAGVLYGPIVGPLVTSIGTILGSAGAYAIGHWGGRPLAERVAGKE
                     RLQQAQDRMGGKRWLVLIPLFLIPFSPADALCFVAGIIEMGWRRFLTAVLLGRIPKDA
                     LVAVAAALGWKALG"
     gene            5788..8556
                     /gene="acnA"
                     /locus_tag="VFR25_05510"
     CDS             5788..8556
                     /gene="acnA"
                     /locus_tag="VFR25_05510"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET9940537.1"
                     /translation="MTDRFASRATLEVGGKSYEIHRLDALTKAGLPVEHLPYSIKILL
                     ENLLRTQDGRTVKAEDVEAVARWEPMAEPSREIQFTPSRVLLQDFTGVPAVVDLAAMR
                     DAMAALGGDAKRINPLQPAELVIDHSVQVDEYGTSAALLINAEKEFERNRERYLFLRW
                     GQRAFDNFRVVPPATGIVHQVNLEFLARVVFATEGKTPRAYPDTLIGTDSHTTMINGL
                     GVLGWGVGGIEAEAAMLGQPVVMLIPQVVGFRLDGKLKDGATATDLVLTVTEMLRKKG
                     VVGKFVEFYGPGLAALPVADRATIANMAPEYGATCGIFPIDQETLRYLEFTGRPAEQL
                     RLIEAYAKEQGLFHTPGSPEASYSDTLQLDLATVEASLAGPRRPQDRVALRDVKKNFQ
                     TALPSMKSTPAAAAKPVPGEGRWENEGGDGAIATMAPAAPRVSHKGEEFPLNEGAVVI
                     AAITSCTNTSNPSVMVGAGLMAKHAVERGLKTKPWVKTSLAPGSKVVTGYLDQAGLTT
                     YLDKLGFQTVGYGCTTCIGNSGPLPEPISNAIEEGSLVVASVLSGNRNFEGRIHPEVR
                     ANYLASPPLVVAYALAGRIDIDLDTEPLGQGSDGKPVFLRDLWPSRRDIEDTIRTAVK
                     SETFRSTYKDVYTGDETWRTLPVPEGDRYAWEPGSTYVKLPPYFEGMERTPKPVTEIT
                     GARVLAFLGDSVTTDHISPAGSIKRASPAGQYLEAQGVKPADYNSYGSRRGNHEVMVR
                     GTFANVRIRNKLVPGVEGGMTAHLPDGETMAIYDAAMRYEKEGIPSIVLAGKEYGSGS
                     SRDWAAKGPKLLGVRAVIAEGYERIHRSNLIGMGILPLQFRTGETAETLGLTGRERYD
                     VVGLGPVIASGSTRGAEITVRAHADQGGTREFRAIVRIDTPQEARYFEHGGILPYVLR
                     RLLGSADR"
     gene            8625..10550
                     /locus_tag="VFR25_05515"
     CDS             8625..10550
                     /locus_tag="VFR25_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9940538.1"
                     /translation="MKTLLSGDQELTLTVICAITGLAAGTADRWAPGAGLRIPLFVVS
                     YLAGGIGPGLDLIRNLLARRLDINLLMVTAATGSAILGHWGEGAVLLFLFSLSGALER
                     RAMERTKRTIEGLIELRPDTALVLRDGREERVPVQDVRVDDRVRAIPGERFAVDGVVV
                     EGQSEADESTITGESIPVEKGPGSRVFAGTMNLRGSPIVRVDKAAEETTLARIIHLVG
                     EARKTKSTAETFVERWELPYVVSVFLGAGLAAGLSLVQGVDAWTACYRGLVLLVAASP
                     CAVVIATPAAILSAITHAAHHGVLFKGGVHLERLAQVTALALDKTGTVTEGRPRVLSV
                     TAVPPAREDDLLRYAAAVEARSEHHTGRAVVAEAARRRLTWPEVTEFESHSGEGVHAH
                     AEGRWIGVGRDPLFRRHEVPVPAEAGREAEAMRARGETALLVIVRHGTNGTPEPSPES
                     GAIAVADAIRSQAPAAIAACRRVGIKYVAILTGDHEQVAQPIARAIGADLVRAGLLPD
                     EKVAALRELRERWPSLAMVGDGVNDAPALASATVGIAMGGAGTDIALETADVVLMRDD
                     LRGIPLALWLARRTQQVVRQSLGLAFGVIGILVISTLTGLLPLWLAVVCHEGSTLLVI
                     ANGLRLLGARHPEFEPA"
     gene            10555..11112
                     /locus_tag="VFR25_05520"
     CDS             10555..11112
                     /locus_tag="VFR25_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HET9940539.1"
                     /translation="MRADAIVVLAGRPERKAYGLELWKGGTAPALVLSVARFEWRAFP
                     ALGLPDDGGLAALVAQTPPRLRHFLVTLNERGAHAEWTPRGRLGTWSEALGTARLARS
                     LGWRSLLLVTTEAHAARARLAFGRALDGSGVAVTLASVPDERSSIRRSGWWKDRWARR
                     VVIGEWIKLPAYWLFARGGPPAPRS"
     gene            complement(11054..11812)
                     /locus_tag="VFR25_05525"
     CDS             complement(11054..11812)
                     /locus_tag="VFR25_05525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HET9940540.1"
                     /translation="MNLIRPWPRDRARRRAYVDDVFERVAPQYDRLTRLLSFGMDDRW
                     KAGLLTLLREEHRDGSVLDLATGTGAFPLLLRREGHRGPIVGVDRSPAMLTRARSKCR
                     GADIRFVEADLNVLPFAPESFDVIVMGYGLRYLDDLPRAMDSVHRVLRPGGMFLTLDF
                     GVPRNALMRGFCFSYLFLVGTLWGLVLHGRADTYWHIVESLEAYPGQDALVDALTRAG
                     FGQLLVAERIGGLSVVTSGERVGLHERRASMPGA"
     gene            11953..13356
                     /locus_tag="VFR25_05530"
     CDS             11953..13356
                     /locus_tag="VFR25_05530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9940541.1"
                     /translation="MAFNALGLPPQILKGVRAAGYSEPTPIQRKAIPIVLEGKDLIGA
                     AQTGTGKTAAFILPILARLLQGPKRLRALVLTPTRELAAQVETNARDYARFTDINVGV
                     VYGGVPLPPQERALRAGVDLLVATPGRLLDLHGRLALRLDYVEVLVLDEADRMVDMGF
                     APDLRRILRLLPHDRQTLLFSATMPPELNQVAREALKEPARVDLAPPSRPAAGITQAV
                     YPVPRNLKTELLDEILSAIDVRSMIVFTRTKHGADRLARQLGKRNYAVAPIHGNRSQS
                     QRERALGDLKRGRIQILVATDIASRGIDVEGISHVVNYDVPHSPEDYVHRIGRTGRVD
                     AVGDAFTLMSPEEQKEFTAIERFLGRAVPRVMLPDFDYKMRPREIADSIRGSDRKGGG
                     GHGGGGRGGRGGRSSGSPAGGRHSRSSNGRAQGGGRTSHGSSSSGQGQGGGQQGQAQF
                     GRRPKAIRGVDRRRGRL"
     gene            13474..13998
                     /locus_tag="VFR25_05535"
     CDS             13474..13998
                     /locus_tag="VFR25_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET9940542.1"
                     /translation="MSPFAALVRSIAYQQLNGRAAATIHGRVLTLYHPRRHPRPEDVL
                     ATADERLRGAGLSRAKILAVKDLAAKTLDGTVPTLARLKRLDDEEIVERLSSVRGVGR
                     WTVEMLLIFRLGRPDVLPATDYGVRQGFKRTFRTRGLPSPAQVLKRGERWRPYRSVAS
                     WYLWRSLEEPAADA"
     gene            complement(14012..17248)
                     /gene="carB"
                     /locus_tag="VFR25_05540"
     CDS             complement(14012..17248)
                     /gene="carB"
                     /locus_tag="VFR25_05540"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9940543.1"
                     /translation="MPRDASIRSVLVIGSGPIVIGQACEFDYSGTQAVKVLRAEGIRV
                     SLVNSNPATIMTDPEYADRTYVEPLTPEVLEAILERDRPDALLPTVGGQTALNLAVEL
                     SRRGILDRLGVRLIGASRRAVEVGEDRKLFKEAMRRIGLDVPQSGFARTREEALQVLE
                     ETGLPAVIRPSFTLGGTGGGIAYNREEFEQAVARGLELSPTHEILIEESVIGWKEYEL
                     EVMRDKADNFVVICSIENFDPMGIHTGDSVTVAPAQTLTDREYQVMRDAARAIIREVG
                     VETGGSNIQFAVDPKTGRLVVIEMNPRVSRSSALASKATGFPIAKIAALLAVGYRLDE
                     IQNDITRVTPASFEPVLDYVVVKIPRWAFEKFRDESPLLGTQMKSVGEVMAIGRTFKE
                     ALQKGIRALETGSGRGGFGRGLDDARIKEKLLSPDPDRIFYVKRACELGWSHEEIHRM
                     TGIDAWFLDQIQQISDMKERIGPGLSEDTLRAAKRMGFSDARLAELLGTTEETVRAQR
                     HALGIRPVYKRVDTCAAEFVSYTPYLYSTYEQECEAEPTDRKKVMILGSGPNRIGQGI
                     EFDYCCCHGSFAFKEEGYETIMVNCNPETVSTDYDTSDRLYFEPLTFEDVMNVIEKEK
                     PEGVVIQFGGQTPLRLAVPLHRAGVKILGTSPDAIDQAEDRRRFSALLSELGLPQPES
                     GTATSLAEAKAVAARIGYPVLVRPSYVLGGRAMAIVYDEAHLEDYAREAVQASPEHPI
                     LIDRFLEDAYEVDVDAVADGERVVIAGILEHIEEAGIHSGDSSMVLPTFKVPARHLAT
                     IKQATRRLGLALGVRGLMNIQFALQGETVYVLEVNPRASRTVPFISKATGVPIAKVAA
                     RVMAGRTLAEQGLTEDLEVDRFFVKASVFPFLKFPGSDVLLGPEMKSTGEVMGISRNL
                     GIAFAKALAATGSTIPTSGTAFVSVNDPDKGSVLPHARALAQMGFKIVATRGTADFLN
                     RQGVPAEMIYKVNEGRPHVVDRIKSRGIDLIINTPLGEESFYDDGAIRKNAILYGVLC
                     LTTLTAAAATVEAIRALRERPLEVTSLQEIHEAKRKPAETLATAESRA"
     gene            complement(17251..18459)
                     /gene="carA"
                     /locus_tag="VFR25_05545"
     CDS             complement(17251..18459)
                     /gene="carA"
                     /locus_tag="VFR25_05545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET9940544.1"
                     /translation="MTHPEEVRATLALEDGICFYGYGFGRQGETTGEVVFQTALCGYQ
                     EVLTDPSYLGQIVVMTYPHIGNYGVNLDDVESARPQVAGFVVREASTRASSWRASGEL
                     HRYLDEAGIVGIQGIDTRALTRHIRTHGAKRGVISTIAHDPEVLVAKARASRSMIGLD
                     LAREATCPEPYRWSHGPAGWQEPVRDLFAPPRDPFHVIAYDFGMKRNILRHLVASGCE
                     VTVVPATTTAREALALEPDGIFFSNGPGDPEPCDYAVEAARELIEARPVFGICLGHQI
                     LGLACGGKTFKLKFGHRGANHPVKNLRTGQVEITSQNHGFAVDPSLFDDPAFELTHVN
                     LNDGTVEGFRHRALPVFSVQYHPEASPGPHDSHYLFREFVAEMAERRDGGSVTVVRGL
                     PPAIAAGAGS"
     gene            18661..19257
                     /gene="msrB"
                     /locus_tag="VFR25_05550"
     CDS             18661..19257
                     /gene="msrB"
                     /locus_tag="VFR25_05550"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9940545.1"
                     /translation="MKWKRVDPVVAVAVAFAVLVGPAVCAATSPGTVDTKAATAAPAK
                     AAVAPAPSSKIKIFSVERKGFVMVDKVKKSDAEWKKLLTPEQYNVTREAGTERAFTGK
                     YWNNHAKGTYRCVACGTELFSSDTKFESGTGWPSFWDPIAKENVQIGKDNSLFMERDE
                     VRCARCDAHLGHVFDDGPKPTGLRYCMNSAALDFKPAK"
     gene            19372..19815
                     /locus_tag="VFR25_05555"
     CDS             19372..19815
                     /locus_tag="VFR25_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9940546.1"
                     /translation="MKILVGVDESPFSQAALDWVKQIPWPSGTSIRVVSASPPVFVGT
                     GEANAPAVVAQLIQDQNRMHAEIAGRAARSLEASGRTVEPVMISGDPRSTLVEEARKI
                     GADLVVVGSHGRSGLSRLVLGSVASYVSSHAPCSVLIVKRPHDAR"
     gene            19866..22103
                     /locus_tag="VFR25_05560"
     CDS             19866..22103
                     /locus_tag="VFR25_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HET9940547.1"
                     /translation="MRDRIGSFRILERLGEGGMGVVYAAQDERLQRRVAIKMIRDDSG
                     LREARDRFWREARSAAKLNHPNVCQIYDVGEEGSDLWIAMELLEGEPLASRMARDPIS
                     LNEAGPIVLGILAALDALHRHQIVHRDLKPSNVFLTSHGVKLLDFGLARPIEDSAQVT
                     TTNLTRTGIVIGTPRYMSPEQWKREPVDARSDIFAAGVILYEMISGAPAFPGQSPGEI
                     RHAALFDQPPALSGPPAVMAVDRVIQRAISKGSADRHPTAQAMADELRSALRLLEAGD
                     APRVRAVTRLIVLPFRALRPDPDCDFLTFSLPDAITGSLAGIDSLVVRSSLTAARFTS
                     ATPDLKQIAEETEVDLVLTGTLMRAGDKVRVNTQLLDAVTGTVAWTLSTQASMGDLFE
                     LQDGLARHIVESLSLPLSARERTSLQKDVPVSAKAYEFYLRANQLAWTSDGWTLARDL
                     YLKCLEADPKYAPAWAQLGRIYRVMAKYLEDNPKENLLRAADAFHRSLEINPDLSLAH
                     NLYVYLEVEMGRATEAMTRLLDRARDRPSEAGLFAGLVQACRYCGLLDASLAAAERAL
                     RLDPNIPTSITYTHWMSGDYERAMQLRANDLPFIRGYSLMSLGRREEALEVFAQMKDM
                     IHLPGFGRSMYHLLVGNREGSLSDIRSVLKSAFLDPEGIYFIARGLAYWGEPEEALRT
                     LDRVVEGGFYCDQTMARDPWLDSIRTTPEFVRILRRAESLRRSAVAAYLEHQGDRILG
                     VRPAN"
     gene            complement(22100..23350)
                     /locus_tag="VFR25_05565"
                     /pseudo
     CDS             complement(22100..23350)
                     /locus_tag="VFR25_05565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(23367..>23762)
                     /locus_tag="VFR25_05570"
     CDS             complement(23367..>23762)
                     /locus_tag="VFR25_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET9940548.1"
                     /translation="LLLFTTGQIDHFFIPQWVVIVCGIAIGLGTLTGGWRIVHTLGAR
                     VTKLRPVGGFCAETAGAISLFGATHLGIPVSTTHTITGAIVGVGATHRLSAVRWGVAG
                     RIVWAWVLTIPASAFGAALTFYITSRLGG"
BASE COUNT         3837 a         8294 c         8168 g         3463 t
ORIGIN      
        1 gtacggatcg tcgggatcgt ccttccgctc gagccgccca gccgtccgcg cccgatcgac
       61 gagatacgag ggacgcccgt acggctcgaa gaggagtcgg tccaccacgg cgtcgtaccc
      121 cgcgcgcacg gccacgcgcg ccatccccgg cgtcacgagc ccgaacagat cgagcaccgg
      181 acggtccgcg tagtagccga aggcgccgat gtccgggagc gcgaagagag tcccgggcgg
      241 cgtctcgtga cgggcccaga ccccgaaggc gacgagcgac gacctgaggc ccgcggtgtg
      301 ccggaccgcg tgcggcacgt tgtagcggat cgtgaggaac gcgctctgcg cgagatagag
      361 taggacgagc gcggtccacg ccagggaccg aagcggaggg cgaaggtaga ctccgcccca
      421 cgccatcgag gccgccccca acaaagcaat cgaaggaagc gccggaagaa gataccgcga
      481 cacgacttga acccctccca tcgcgagccc gaacaggagc gcgaggggcc acgccgagac
      541 cgcgatccag aacgctcgcc gctccggcga ctgcttccgc agcgcctcgg gcccggcgag
      601 cgcaagcccc acgatcgcga gcccgatcgg aagcgcgtcg gtcgcgagca ggatccggag
      661 cgacgtgcgg agcgccgacg tggccagctc gagatccccg aaggcccccg ctttggccgc
      721 gctcgtgttg gggaggatcc ggtggaacca ggcccacgcg gtgaggagcc acggcccggc
      781 ggcgaggagc gctcccccga agagaccgac gagccgcttc cgcgtggtcg cattcgcgcc
      841 gaccgccacc gccgcgagga aggcgaggag cacgcactcg ggacgcgcga ggaaggcgag
      901 gccgaggagg atgccggccg gccaggcgct tcgattctcg aggaccgtcc gcgcgagggc
      961 gtcgagcgaa gccaccacga ggaagatcgc gagcggcgtc tccatgccgg atccggtcca
     1021 ccgggcgacc cacgcattcg ccgcgaggaa aaacggcgcg aggagtgcct gccgcgagga
     1081 ccagccgaga cggcgtccta gccggtagag aaggaggatg gcgccgagca ggctgagcaa
     1141 gccccacatc ttggcgatcc cggcgagcgg cggaagcgcg gccggatcga gcggggtcgc
     1201 ggagcttccc ggcacggcca gaccggtcag cgcgatcaag agcacccaga gtggactcgt
     1261 ggcgccgtac gtcggctccc ccgcgttgaa cgagaagccg tgccccgcga ggagattctt
     1321 cgcgaactgc atgtggatga aggtgtcgtc ggtgagatag ccgcggaaca gaaacacgtg
     1381 ggcgatcgcg agagccagaa ttcccgcgag cgcgattcga gcgggcgccc aagggcgccc
     1441 ctgagggctc accggctcgg gagccaccgc tcgattcgat ccgccaccgc gttcggcgtg
     1501 aagccgaatt ccttcgcgat cacctcggcc ggagccgacg cgccgaaatg atcgagcccg
     1561 atccggaggg aatccgcgcc gacgagcgag caccagagat cggtctgcgc cgcctcgacg
     1621 accacgaacg ctccatcggg aggaaggacg ttccggcggt agtcctccgc ctgccgctgg
     1681 aagacctcga cacacggcat cgagaccacg cgcgccgcga ttcccttctt ggcgaggaga
     1741 tccgcggccg agacggcgag cggcgtctcc gagccggacg ccacgagcgt cacgggtgtc
     1801 ccccccgcgg acggctcgcg cagcacgtag ccgccgcgcg ccgcgagatc ggcggagccg
     1861 cccaccggcg ccagcttctg ccgcgtgagc atgagcgcgg tcggaccgtc cgcgcgacgg
     1921 atcgcggagg cccacgccgc tgcggtctcg ggaccgtctg ccggacgcca cacgtcgagg
     1981 ttcggcatga gccggagcga ggggagctgc tcgatcggct ggtgcgtggg gccgtcctct
     2041 ccgagaaaga cgctgtcgtg cgtgaagacg aagatcgatc gcagcttgct gagcgccgca
     2101 agacggatcg acgggcgcat gtaatccgag aagacgagga aggtcgcccc gtacgggatg
     2161 tagccgccat ggagcgcgat cccgttcacg atggcgccca tgccatgctc gcgcacgccg
     2221 aagtggaggg ttcgtccgga gtagtcgacc ggcgcctcga tctcgagcgc ttcccggccg
     2281 acgcttcctc cctggtcgat gtcggtgatc gtcgagggac tgagatcggc cgagcccccc
     2341 acgagccacg gcaccgcgcg cgccgcggct tgaatgacct tgcccgacca cgctcgcgtc
     2401 gcgagcgagc tcttggtgtc gaccgcagcg aggaggcgcg cctccagatc ttctggaagg
     2461 tcgagccgcg cagccgactc gaagcggcgt gccgcctcgg gcgcgcgcgc ccggagcgcg
     2521 tcgtagcggc ggttccactc ctcgcgagcg gcgcgcttct cctcggagag cgcgcggaat
     2581 cgctcgagcg cgtcggacgg gatgtagaac tcgggggtga gcggccagcc ggccgcctcc
     2641 ttcgcccgct tgagctcctc cgcccccagc ggctcgccgt ggaccttggc ggtgcccgcc
     2701 ttggtcggcg ccccttttcc gatcagcgtg cgcgcgagga tcagcgatgg acgcttcgtc
     2761 tcctggagag cttcctcgat cgcttcctcg atctggcggt gttcgtgccc atcgatccgc
     2821 ttcgtgtgcc atccgtacgc gtcgaagcgc ttcgccgcgt cctcggtgaa ggcgaggccc
     2881 gtcgaccctt cgatcgagat gtgattgtcg tcgtagagat agatcaggtt gccgagccgg
     2941 agatgacccg cgagcgaggc cgcttccgac gcgacccctt ccatgagatc cccgtcgctc
     3001 acgatcccga acacgcggga cgcgagcgcc ccctcgagct cgggatagcg cgtgagcgtc
     3061 atctggtgcg caagcgccat gccgactcca ttgccgaaac cctggccgag cggccccgtc
     3121 gtggcctcca cgccgggcgc gtgcccccgc tccggatgcc cgggcgtgcg ggagccccac
     3181 tggcggaact ggcggatatc gtcgagcgtg agatcgaagc cgaagaggtg gaggagcgag
     3241 tagaggagca tcgacgcgtg gccgttcgac aggacgaacc ggtcgcgccc gagccagtgc
     3301 gggtccttgg ggtcgaagcg aaggaatttc gaccagagca cgaacgccat gtcggcgccg
     3361 cccatgggag cgccggggtg ccctgacttc gccttctcca cggcgtccgt ggaaaggaac
     3421 ttgatcgtct ccacgcagag ctggtccagc tcggcgccta tcgtcgccat tcgtcctccc
     3481 ggcccaggac ccagttcgtc ggcgggaaac cgacgtaacg gtgtatcatc acctgagttc
     3541 cactttagcg tgtgagggca tgggaggtcg acaatgatcg gaaacgacat ccggtcggct
     3601 gggtggtccg ggcttcttct gggggtcctc gtcctggggg cggccgtggg cggctgcgcg
     3661 accaatccgg cgacgggggc gaagcagatt tcgctcgtct cgcgggggca ggagctggac
     3721 atggggcgcg aggccgaccc ggccatcatc agcgagtacg ggctctacga cgatccgaag
     3781 ctcgcggcct acgtcgattc cgtgggccag cgcctgggca aggtctccca cctgccgact
     3841 ctcgtgtggc acttccggct cctcgattcc cccgtcgtca acgccttcgc cctcccgggc
     3901 ggctacatct acatcacgcg cggcattgtg gcccagatga actccgaggc ccagctcgcc
     3961 ggagtcatcg gacacgagat cggccacgtg accgcgcgcc actcggcgca gcagatcacg
     4021 aaccagcaga tcgcccaggt gggactcctc gtcgggatgg tcgccttctc gggtttccgg
     4081 gagtacggag gtctcgccgc ccaggggatg gggctcctct tcctcaagta cagtcgcgac
     4141 cacgagacgc aggcggacga gctgggcgtc cagtacaccg cgcgcgcgaa gtacgatcct
     4201 cggcagatcc cggcgacgta cgagacgctg aagcggatcg gggatcgcga aggggccggg
     4261 ctgccgaact tcctgtccac ccaccccgat cccggcgacc ggcaggtccg tacacgccag
     4321 ctcgccgagg ccaccgtggg aggcgcggcg ctcaatctcg agatcggcgc cgccaactac
     4381 cgacggcaca tcgaggggct ggtttacgga gacgatccga gggccggata cttcgagaac
     4441 agcaggttct accacccgca gctcggattt cagctcatct tcccgagcgg ctggaagacc
     4501 gtgaacaatc cctccgccgt cgtcgcgacg aacgagagca tgggcgcgac gatgcagctc
     4561 acgctcggct cgacgaagaa cgagagcctc acgccgtcgg agtacgtgga ctcgcttcgc
     4621 gtgaagaacg cgatcacttc cgcctccggc cacccggagc agttccgcga ctaccccgcg
     4681 tgggtcggaa acgtcgtgat gtcgggcgac cagggaggca acgtcgccgc cgggttcgtg
     4741 cgcatcaagc caggacaatt cctcgagatc atcggacagt ccaagggcgg cgccgcgacg
     4801 gagcagatct tcgccgccat tcgcagcgtg gccgcgctga ccgatccgga gaagctcaac
     4861 gtcacgcccg atcgcatcgt catcaagccc gcgccacgca cggccacgtt ttcggagcta
     4921 tggggcgaga tgggtggcgg ggccatcggc gtggaggacg gcgcgatcct caacagcacg
     4981 cgtgccacaa cgacgattca agccgggacg cccatgaaga ccgtagttcg cggaaagcat
     5041 gcctagaacg tactgagcct accctacccc agggctttcc agcctagagc tgcggctacg
     5101 gccaccaggg cgtccttggg gattcgtcct agtagcaccg cggtgaggaa tcgcctccat
     5161 cccatctcga tgatgccggc cacgaagcag agtgcgtcgg cgggcgagaa tgggatcagg
     5221 aagagcggga tgagcaccaa ccagcgtttt ccgcccattc gatcctgtgc ctgctggaga
     5281 cgttcctttc ccgcaactcg ttcggccaag gggcggccgc cccagtggcc gatggcgtag
     5341 gcgccggcgg agccgaggat cgttccgatg gaggtgacga gcgggccgac gatcggaccg
     5401 tagaggacgc cggcgatgac ggggacgaag acgccgggga tgggggcggc gaggatctgt
     5461 cccgcctgga tggcgagcag gaggagcggg ccgtagacgc cggcgctccg gatccaggct
     5521 tcgatctccg cgcgatggat tcgcggcgcg agaaggatgc ttccctcgat cagcacgccc
     5581 gcggccacga cagcgatcgc aatccacagc caacgacgtc gcgctgccga gttccggtcg
     5641 ctcaccgcgc ggactctacg tcaccctcgc atgcgccgaa agtatgatct ggaaggccgt
     5701 tccggccgac tccgtggtcg tttatcctag ggtcgatcga cggtcccttt cccgcgccct
     5761 gaccgggcgc ctcacccgga gatcccgatg accgatcgct tcgcctcccg agcgaccctc
     5821 gaggtcggcg gcaagagcta cgagatccat cgcctggacg ccctcaccaa ggcgggcctt
     5881 ccggtcgagc acctccccta ctccatcaag atcctgctcg agaacctgct ccgcacgcag
     5941 gacgggcgaa cggtgaaggc agaggacgtg gaagccgtcg cgcgatggga gccgatggcc
     6001 gagcccagcc gcgagatcca gttcacgccg agccgcgtgc tgctccagga cttcaccggc
     6061 gtgcccgccg tggtcgatct cgccgccatg cgcgacgcca tggcagcgct tggcggggat
     6121 gccaagcgca tcaatccgct ccagccggcg gagctcgtca tcgaccattc ggtgcaggtg
     6181 gacgagtacg gcacgagcgc ggcgctcctc atcaacgcgg aaaaagagtt cgagcggaac
     6241 cgcgagcgct atctcttcct aaggtggggc cagcgcgcgt tcgacaactt ccgcgtcgtg
     6301 cctcccgcga ccggcatcgt gcaccaggtg aacctcgagt tcctggcgcg ggtggtgttc
     6361 gccaccgagg ggaagacgcc ccgggcctat cccgacacgc tcatcggcac cgattcccat
     6421 accacgatga tcaacggtct cggcgtcctc ggatggggcg tgggcgggat cgaggccgag
     6481 gccgcgatgc tcggccagcc ggtcgtgatg ctgatcccgc aggtggtcgg cttccggctc
     6541 gacggcaagc tcaaagacgg cgcgaccgcg accgatctcg tgctcaccgt caccgagatg
     6601 ctccgcaaga agggcgtggt cggaaagttc gtcgagttct acggccccgg cctcgccgcg
     6661 cttcccgtcg cggaccgcgc gaccatcgcg aacatggctc ccgagtacgg agccacgtgc
     6721 ggcatcttcc cgatcgacca ggagacgctc cgctatctcg agttcacggg acgccccgcg
     6781 gagcagctcc gcttgatcga ggcgtacgcg aaggagcagg gactcttcca cacgccgggc
     6841 tcccctgagg cttcgtactc ggacacactc cagctcgacc tcgcgacggt cgaggcctcg
     6901 ctcgcggggc cgcgacggcc gcaggaccgc gtcgccctgc gcgacgtgaa gaagaacttc
     6961 cagacggcac tcccgagcat gaagagcacg cccgccgccg cggccaagcc ggtccccggc
     7021 gagggacggt gggagaacga agggggcgac ggagccatcg cgacgatggc gccggccgcg
     7081 cctcgggtgt cgcacaaggg cgaggagttc cctctcaacg aaggcgcggt cgtgatcgcg
     7141 gcgatcacga gctgcaccaa cacgtcgaat ccctcggtga tggtcggcgc cgggctcatg
     7201 gcgaagcacg cggtcgagcg cggtctcaag acgaagccct gggtgaagac gagcctcgcg
     7261 cctggctcca aggtcgtgac cgggtacctc gaccaggccg ggctcacgac gtacctcgac
     7321 aagctcggat tccagaccgt cgggtacggc tgcacgacct gcatcgggaa cagcggcccc
     7381 ctgcccgagc cgatctcgaa cgccatcgag gaagggagcc tcgtcgtcgc gtcggtcctg
     7441 agcggaaacc ggaacttcga gggacgcatc catcccgagg tgcgcgcgaa ctacctcgcg
     7501 tctcctcccc tcgtcgtcgc ctacgcgctc gcgggacgca tcgacatcga cctcgacacg
     7561 gagccgctgg gccagggctc cgatgggaag ccggtcttcc tccgggatct ctggccgtcg
     7621 cgcagggaca tcgaggacac gatccgaacc gcggtgaagt cggagacgtt ccgctccacc
     7681 tacaaggacg tctacaccgg agacgagacg tggcgcacgc tccccgttcc cgagggagac
     7741 cgctacgcgt gggagccggg ctcgacctac gtgaagcttc ctccctactt cgagggaatg
     7801 gagcgcacgc cgaagccggt caccgagatc acgggcgcgc gcgtcctcgc cttcctcggc
     7861 gacagcgtca cgaccgacca catctcgccg gccggatcga tcaagcgcgc gtctcccgcc
     7921 ggacagtacc tcgaggcgca aggggtgaag ccggccgact acaactcgta cggctcgcgc
     7981 cgcggcaatc acgaggtcat ggtgcgcggg acgttcgcga acgtgcgcat ccggaacaag
     8041 ctcgtccccg gcgtcgaggg aggaatgacc gcgcatctcc cggacggcga gaccatggcg
     8101 atctacgacg cggcgatgcg ctacgagaag gaagggatcc cgtcgatcgt gctcgccggc
     8161 aaggaatacg gctccggctc ctcgcgcgac tgggccgcga agggcccgaa gctcctcggc
     8221 gtgcgtgccg tgatcgcgga gggatacgag cggatccacc ggagcaatct catcgggatg
     8281 ggcatcctcc cgctccagtt ccgcaccgga gagacggcgg agaccctcgg cctgacgggc
     8341 cgggagcgat acgacgtcgt cgggctcggt ccggtcatcg cctcaggctc gacccgcggc
     8401 gccgagatca cggtgcgcgc gcacgcggat caaggcggca cgcgcgaatt tcgcgcgatc
     8461 gtccggatcg acacgcccca ggaggcgcgc tatttcgagc acggcgggat cctcccctac
     8521 gtgctccgac ggctcctggg atcggcggac cgctgagcgc cggagcgtcg gcgagcgcgg
     8581 ccggctccgt tcggcgccgt gcgccgggcg ggatcaaggc gcggttgaag acgctcctct
     8641 cgggcgacca ggagctcacg ctcaccgtca tctgcgccat caccggcctc gccgccggga
     8701 cggccgaccg gtgggcgccc ggcgcgggac tccggattcc gctcttcgtc gtctcctacc
     8761 tcgcgggggg catcggtccc ggactcgacc tgatccggaa tctcctcgca cgccgcctcg
     8821 acatcaacct cctcatggtc acggcggcga ccggatcggc gatcctcggc cactggggcg
     8881 agggggcggt cctcctcttc ctcttctcgc tgagcggcgc gctcgagcgc cgcgcgatgg
     8941 agcggacgaa gcggacgatc gaggggttga tcgagctccg ccccgacacc gccctcgtcc
     9001 ttcgcgacgg acgcgaggag cgcgtgccgg tccaggacgt gcgcgtggac gatcgcgtgc
     9061 gcgccattcc cggcgagcgg ttcgcggtgg acggcgtcgt ggtcgagggc cagtccgagg
     9121 cggacgaatc cacgatcacg ggcgaatcga tccccgtgga gaaggggccg ggaagccgcg
     9181 tgttcgcggg gaccatgaac ctgcgcggct cccccatcgt ccgagtcgac aaggcggccg
     9241 aggagaccac gctcgcgcgc atcatccatc tcgtcggcga ggcgcggaag accaagtcca
     9301 ccgccgagac gttcgtggag cgctgggagc ttccctatgt cgtctcggtc ttcctcggcg
     9361 cgggactggc cgccgggctg tcgctcgttc agggggtgga cgcctggacc gcgtgctacc
     9421 gggggctcgt gctgctcgtg gcggcgtctc cctgcgccgt cgtcatcgcg actccggccg
     9481 cgatcctctc cgcgatcacg cacgcggcgc accacggcgt cctcttcaag ggaggcgtcc
     9541 atctggagcg gctcgcgcag gtcaccgcgc tcgcgctcga caagaccggc accgtcaccg
     9601 aaggacgacc gcgcgtcctc agcgtgaccg cggttccgcc tgcgcgcgag gacgatctcc
     9661 tgcgctacgc agccgccgtg gaggcccgga gcgagcatca cacgggacgg gccgtcgtcg
     9721 ccgaagctgc gcggcgccgg ctgacctggc cggaggtcac cgagttcgag agccacagcg
     9781 gtgaaggcgt gcacgcacac gccgaaggcc gctggatcgg cgtcggccgc gatcccctgt
     9841 tccggcgcca cgaggtgccc gtgcccgccg aggcggggcg cgaagcggag gcgatgcgcg
     9901 cgcgcggcga gaccgcgctc ctcgtcatcg tgcggcacgg cacgaacggc acgcccgaac
     9961 cgtcgccgga gagcggcgcg atcgcggtcg cggatgcgat ccgatcgcag gcgcccgcgg
    10021 cgatcgccgc ctgccgcagg gtgggaatca agtacgtcgc gattctgacg ggcgaccacg
    10081 agcaggtggc gcagcccatc gcgcgcgcca tcggcgccga cctggttcgc gcgggactcc
    10141 ttcccgatga gaaggtcgcc gcgcttcgcg agctgcgcga gcgctggccc tcgctcgcga
    10201 tggtgggcga cggcgtgaac gacgctccgg cactcgcgag cgcgacggtc gggatcgcca
    10261 tgggcggcgc cggcaccgac atcgcgctcg agaccgccga cgtcgtcctg atgcgggacg
    10321 acctccgcgg cattccgctc gcactctggc tcgcgcgccg aacccagcag gtcgtgcgtc
    10381 agagcctcgg gctcgcgttc ggcgtgatcg ggatcctggt catctccacg ctgaccggcc
    10441 tcctcccgct gtggctcgcg gtcgtctgcc acgaagggag cacgctcctc gtgatcgcga
    10501 acggtctccg gctcctgggc gcgcggcacc ccgagttcga gccggcctga tccgttgagg
    10561 gcggacgcga tcgtcgtcct ggccggacga ccggagcgga aggcctacgg gctggagctc
    10621 tggaagggag gcactgctcc tgcgctcgtc ctgagcgtcg cccgcttcga gtggcgagcg
    10681 tttcccgcgc tcggcctccc cgacgacggc ggcctcgccg cgctcgtggc gcaaacgcct
    10741 ccccgcctcc ggcacttcct cgtcacgctc aatgagcgcg gcgcccacgc cgaatggacg
    10801 ccgcgcgggc gcctcggcac ctggagcgaa gccctgggca cggcgcggct cgcccggtcg
    10861 ctcggatgga ggtcactcct cctcgtgacg acggaagccc atgcggcccg ggctcggctc
    10921 gcgttcggac gcgcactcga cggaagcggc gtcgcggtca ctctggcctc ggtgccggac
    10981 gagcgctcga gcattcgacg ctcggggtgg tggaaggatc gatgggcgcg gcgggtcgtg
    11041 atcggggagt ggatcaagct cccggcatac tggctcttcg ctcgtggagg cccacccgct
    11101 ccccgctcgt gaccaccgag agccccccga tccgctccgc gacgaggagc tgtccgaatc
    11161 cggcccgggt gagagcgtcg accagtgcgt cctgccccgg atacgcctcg agcgactcta
    11221 cgatgtgcca gtaggtgtcg gcgcgtccgt ggaggacgag cccccagagc gttccgacca
    11281 ggaagagata cgagaagcag aatccacgca tgagcgcgtt ccgcggcacg ccgaaatcga
    11341 gcgtgaggaa catcccgccc ggccgaagca cccggtgcac cgaatccatc gcgcgcggca
    11401 gatcgtcgag ataccggagc ccgtacccca tcacgattac gtcgaaggac tcaggcgcga
    11461 acggcaacac gttgaggtcg gcctccacga agcggatgtc cgctccgcgg cacttcgacc
    11521 gcgcacgcgt gagcatggca gggctccggt ccacccctac gatcggtccg cggtgtccct
    11581 cgcgccggag gaggagcggg aaagcgccgg tcccggtcgc gagatcgagc acgcttccat
    11641 cccggtgctc ctcgcgcagc agcgtgagga gccccgcctt ccatcgatcg tccatcccga
    11701 acgagaggag tcgcgtgagg cggtcgtact ggggggcgac gcgctcgaac acgtcatcga
    11761 cgtaggcgcg ccgacgagct cgatcgcggg gccagggacg gatcaggttc atcggtccat
    11821 cctaacccgg aatggcgcgc aggaatgcgc ggttgtgata cctcggccga gcgggtacct
    11881 tctttcgacg ccgtgccgag ggcgcggctc acgcccggtt gcctccgggc ccgtctcaca
    11941 ccagaggact tcatggcatt caacgcactc ggccttccgc cgcagatcct gaaaggggtc
    12001 cgcgcggccg gctattccga accgacgccc atccagagaa aagccatccc catcgtgctc
    12061 gagggcaagg acctcatcgg ggccgcacag accggaaccg gcaagaccgc ggccttcatc
    12121 cttccgatcc tggcacggct ccttcaggga cccaagcggc tccgagcgct cgtcctgact
    12181 cccacccgcg agctggcggc tcaggtcgag acgaatgcgc gcgactacgc gcgcttcacg
    12241 gacatcaacg tgggcgtggt ctacggcggc gtgccgctgc cgccccaaga gagggcgctc
    12301 cgcgcgggcg tcgacctgct cgtggcgacg ccgggccggc tcctcgatct ccacggacgc
    12361 ctcgcgctcc gcctcgatta cgtcgaggtg ctggtgctgg acgaggccga ccgcatggtg
    12421 gacatgggct tcgcgccgga cctccggcgc atcttgaggc tcctgcctca cgaccgccag
    12481 acgctcctct tctccgcgac catgcccccg gagctgaacc aggtggcccg cgaagcactc
    12541 aaggagccgg cgcgcgtcga tctggctccc ccgtcgcgcc ctgccgcagg catcacgcag
    12601 gccgtctatc cggtcccgcg gaacctcaag accgagctcc tcgacgagat cctctccgcg
    12661 atcgacgtgc ggagcatgat cgtcttcaca cgcaccaagc atggcgccga ccggctcgcg
    12721 cgccagctcg gcaagcggaa ctatgccgtc gcgccgattc acgggaaccg cagccagagc
    12781 cagagggagc gcgcgctcgg cgatctcaag cgcggccgga tccagatcct ggtcgcgacc
    12841 gacatcgcgt cgcgcggcat cgacgtcgag ggaatcagcc acgtcgtgaa ctacgacgtg
    12901 ccgcactccc ccgaggacta cgtccaccgg atcggacgga ccgggcgggt cgacgcggtc
    12961 ggcgacgcct tcacgctgat gagcccggag gagcagaagg agttcaccgc catcgagcgt
    13021 ttcctcggtc gcgcggtccc gcgcgtcatg cttcccgact tcgactacaa gatgcggccg
    13081 cgcgagatcg cggacagcat tcgcggctcc gaccggaagg gaggcggcgg ccacggaggt
    13141 ggaggacgcg gcggccgcgg cggccgcagc agtggatcgc ccgcaggggg gcgccacagc
    13201 cgatcgtcca acggccgcgc ccaaggcggc gggcggacct cgcacggctc ttcctcgagc
    13261 gggcaagggc agggcggcgg gcagcagggg caagctcaat tcggccgcag gcccaaggcg
    13321 atccgtgggg tcgaccggag gcgcggcaga ctctagcgcg tgcccctctc gttcgacctg
    13381 ggcgtagcag aagcgcacat ccgctccgcc gatcccaagc tcgccgccgt gatggacctg
    13441 catggccgct gccgcattga tcccgatggc ctgatgagcc cgttcgcagc gctcgtccga
    13501 tcgatcgcct accagcagct caacgggagg gccgcggcca cgatccatgg ccgtgtgctc
    13561 acgctctacc atccgcggcg ccatccgcgt cctgaggacg tgctcgcgac cgccgacgag
    13621 cgattgcgcg gcgccggcct ctcccgcgcg aagatcctcg ccgtgaagga tctcgccgcg
    13681 aagaccctcg acggcacggt gccgacgctc gcgcggctca agagactcga cgacgaggag
    13741 atcgtggagc ggctgtcgtc cgtgcgcggc gtgggccgct ggacggtgga gatgctcctc
    13801 atcttccggc tcgggcggcc ggacgtgctc ccggcgaccg actacggagt gcggcaggga
    13861 ttcaagcgga cgtttaggac gcgcggtctg ccatccccgg cgcaggtgct gaagcggggc
    13921 gagcggtggc gtccgtatcg aagcgtcgcg agctggtatc tctggcgctc gctcgaggag
    13981 ccggccgccg acgcctgacc gccgagcgta gctaggctcg actctccgcg gttgcgagcg
    14041 tctcggccgg ctttcgtttc gcctcgtgga tctcctgaag gctcgtcacc tcgagcgggc
    14101 gctcgcggag cgcgcggatc gcctccaccg tcgcggccgc ggcggtgagc gtggtgaggc
    14161 agagcacgcc gtagaggatg gcgttcttcc ggatggcgcc gtcgtcgtag aaggactcct
    14221 cgccgagcgg cgtgttgatg atgaggtcga tgccccggct cttgatccgg tccaccacgt
    14281 gcggccgccc ctcgttcacc ttgtagatca tctcggccgg cacgccttgc cggttcagga
    14341 agtccgccgt gccgcgggtc gccacgatct tgaagcccat ctgggcgagc gcgcgcgcgt
    14401 gcggcagcac gctccccttg tccggatcgt tcacgctcac gaacgccgtc cccgaggtcg
    14461 ggatcgtcga gccggtggcg gcgagcgcct tggcgaacgc gattccaagg ttccgagaga
    14521 tccccatcac ctcgcccgtc gacttcatct cgggaccgag gagcacgtcg gatcccggga
    14581 acttgaggaa cgggaagacc gacgccttca cgaagaagcg gtcgacctcg agatcctcgg
    14641 tcagcccctg ctccgcgagc gtccgtcccg ccatcacgcg cgccgccacc ttggcgatcg
    14701 gaacgccggt cgccttcgag atgaagggca cggtgcggga cgcgcgcgga ttcacctcga
    14761 gcacgtagac cgtctccccc tggagcgcga actggatgtt catgagaccg cgcacgccga
    14821 gcgccaagcc gagccgccgc gtcgcctgct tgatggtggc gaggtggcgc gccggcacct
    14881 tgaacgtggg aagcaccatc gacgaatcgc cgctgtggat cccggcctcc tcgatgtgct
    14941 cgaggatgcc cgcgatcacg acccgctctc cgtcggcgac cgcgtccacg tccacctcgt
    15001 acgcgtcctc gaggaagcgg tcgatcagga tcggatgctc gggtgaggcc tggacggcct
    15061 cgcgcgcgta gtcctcgagg tgcgcctcgt cgtagacgat cgccatggcg cggccgccga
    15121 ggacgtagga gggacgcacc agcacgggat atccgatccg cgcggcgact gccttcgcct
    15181 ccgcgagcga ggtggcggtg ccgctctccg gctgcgggag gccgagctcg gagaggagcg
    15241 cgctgaagcg gcggcgatcc tccgcctgat cgatcgcgtc gggactcgtg ccgaggatct
    15301 tgacgcccgc gcgatgcagc gggaccgcga gcctcaacgg ggtctggccg ccgaactgga
    15361 tgaccacccc ctcgggcttc tccttctcga tcacgttcat cacgtcctcg aacgtgagcg
    15421 gctcgaagta gagccggtcc gacgtgtcgt agtcggtcga gaccgtctcg ggattgcagt
    15481 tcaccatgat cgtctcgtag ccctcctcct tgaaggcgaa cgagccgtgg cagcagcagt
    15541 agtcgaactc gatcccctgc ccgatccggt tcggtccgct ccccaagatc atgaccttct
    15601 tccgatcggt gggctcggcc tcgcactcct gctcgtacgt cgagtagagg tagggggtgt
    15661 aggagacgaa ttcggcggcg cacgtatcca cgcgcttgta gacgggacgg atgccgagcg
    15721 catgccgctg ggcgcggacc gtctcctcgg tcgttccgag gagctccgcg aggcgcgcgt
    15781 cggagaagcc catccgcttc gccgcgcgga gcgtgtcttc cgagagaccg gggccgatcc
    15841 gctccttcat atccgagatc tgctggatct gatcgaggaa ccaggcgtcg atccccgtca
    15901 tccggtggat ctcctcgtgc gaccagccga gctcgcaggc gcgcttcacg tagaagatcc
    15961 ggtcggggtc gggcgagagg agcttctcct tgatccgcgc gtcgtcgagt ccgcggccga
    16021 agccgccccg gccggagccg gtctcgagcg cgcgaatccc cttctggagc gcctccttga
    16081 acgtgcggcc gatggccatc acctcgccca ccgacttcat ctgcgtgccc aggagcgggc
    16141 tctcgtcgcg gaacttctcg aacgcccacc ggggaatctt cacgacgacg tagtcgagca
    16201 ccggctcgaa gctcgccggc gtcacgcgcg tgatgtcgtt ctggatctcg tcgagacggt
    16261 agccgacggc gaggagcgcc gcgatcttcg cgatcgggaa gcccgtcgcc ttgctcgcga
    16321 gcgcggagga gcgggacacg cgggggttca tctcgatcac cacgagccgc ccggtcttcg
    16381 gatcgacggc gaactggatg ttggatccgc cggtctcgac cccgacctcg cggatgatgg
    16441 cgcgcgcggc gtcgcgcatg acctggtact cacgatcggt gagggtctgc gccggcgcca
    16501 ccgtcacgga gtcgccggtg tggatcccca tcggatcgaa gttctcgatc gagcagatga
    16561 cgacgaagtt gtccgccttg tcgcgcatca cctcgagctc gtactccttc cagccgatca
    16621 ccgactcctc gatcaggatc tcgtgggtcg gcgagagctc gagaccgcgc gcgaccgcct
    16681 gctcgaactc ctcgcggttg taggcgatgc cgccgccggt gccgccgagc gtgaacgagg
    16741 ggcggatgac ggccgggagc cccgtctcct cgagcacctg gagcgcctcc tcgcgcgtcc
    16801 gcgcgaagcc gctctgcggg acgtcgaggc cgatccggcg catcgcctcc ttgaagagct
    16861 tccgatcctc ccccacctcg acggcgcggc ggctcgcgcc gatcaggcgc acgccgagac
    16921 gatcgagaat gccgcggcgg gacagctcga ccgcgagatt caacgcggtc tggccgccga
    16981 ccgtggggag gagcgcgtcg gggcggtccc gctcgaggat ggcctcgagc acctccggcg
    17041 tgagcggctc cacgtacgtg cggtccgcat attcgggatc ggtcatgatc gtcgcgggat
    17101 tcgagttcac gagggagacg cggatgccct cggcgcgcag caccttcacc gcctgcgtgc
    17161 cggagtagtc gaactcgcac gcctgcccga tgacgatggg acccgagccg atgacgagga
    17221 ccgagcggat ggacgcgtcg cgcggcaccg tcagctcccc gccccggcgg cgatcgccgg
    17281 tggcagcccg cgcacgaccg tgaccgagcc gccatcgcgg cgctcggcca tctcggcgac
    17341 gaactcgcgg aagagatagt ggctgtcgtg gggcccgggg ctcgcctcgg ggtggtactg
    17401 gaccgagaag accgggaggg cgcggtggcg gaagccctcg acggtcccgt cgttcaggtt
    17461 cacgtgcgtc agctcgaacg cgggatcgtc gaagagcgag ggatccaccg cgaagccgtg
    17521 gttctgggag gtgatctcga cctgcccggt gcggaggttc ttcaccggat gattcgcgcc
    17581 acgatggccg aacttgagct tgaacgtctt cccgccgcac gcgagcccga ggatctggtg
    17641 gccgaggcag atgccgaaca cggggcgcgc ttcgatcagc tcgcgcgccg cctcgaccgc
    17701 gtagtcgcag ggctcgggat cgccgggacc gttcgagaag aagattccgt ccggttcgag
    17761 cgcgagcgct tccctggccg tggtcgtggc gggcaccacg gtcacctcgc atccgctggc
    17821 gaccaggtgg cgcaggatgt tccgcttcat gccgaagtcg taggcgatca cgtggaaggg
    17881 atcgcggggc ggagcgaaga gatcgcgcac cggctcctgc cagccggcgg gtccgtggct
    17941 ccagcggtag ggctcggggc aggtcgcctc gcgcgcgaga tcgagtccga tcatcgatcg
    18001 ggacgcgcgc gccttcgcca cgagcacctc ggggtcgtgc gcgatggtgg aaatgactcc
    18061 gcgcttggcg ccgtgggtgc ggatgtgccg cgtgagggcc cgcgtgtcga tcccctggat
    18121 gccgacgatg cccgcctcgt cgaggtagcg gtggagctcg cccgaggcgc gccacgacga
    18181 ggcccgcgtc gaggcttcgc gcaccacgaa cccggcgacc tgcggccgcg cggactccac
    18241 gtcgtcgagg ttcaccccgt agttcccgat gtgggggtag gtcatgacga cgatctggcc
    18301 cagataggat ggatcggtca gaacttcctg gtacccgcac agggcggtct ggaacacgac
    18361 ctcacccgtc gtctcacctt gtcgcccgaa cccatagcca tagaagcata tcccgtcttc
    18421 gagcgcgagc gtcgcgcgga cctcttccgg gtgcgtcacg ggcctcctcg atgttcgtac
    18481 ttcggggtgg aagggacgaa ggggtcgggg ggccaaggca tctcggtacc gaccgttgcg
    18541 cactatactg gagcggacgg gacgaacgcg agcgcgatgg aaccgaacgc tcagattccg
    18601 gttcggacgt acaggttcgg cacgctcgtc gggcccgttc accaacgaag gagcctcctc
    18661 atgaaatgga agcgcgtcga cccggtcgtg gctgtcgcgg tggcgttcgc cgtcctcgtc
    18721 ggcccagccg tctgcgcggc tacctccccg ggcacggtgg atacgaaggc ggcgaccgcc
    18781 gccccggcca aggcggccgt ggcgcccgcg ccgtcctcga agatcaagat cttctccgtc
    18841 gagcgaaagg ggttcgtcat ggtggacaag gtcaagaagt ccgacgcgga gtggaagaag
    18901 ctcctgacgc cggagcagta caacgtgacc cgcgaggccg gcacggagcg cgcgttcacc
    18961 ggcaagtact ggaacaacca cgccaagggc acctaccgat gcgtcgcctg cggcacggag
    19021 ctcttcagct cggacacgaa gttcgagtcg ggcacgggct ggcccagctt ctgggatccg
    19081 atcgcgaagg agaacgtcca gatcggcaag gacaactccc tcttcatgga gcgggacgag
    19141 gtccgctgtg cgcgctgcga cgcgcacctc gggcacgtgt tcgacgacgg ccccaagccg
    19201 accggcctcc gctactgcat gaactcggcg gccctcgact tcaaacccgc caagtgagtg
    19261 agcgcggcgc cggcgtagga gccggcgccg tagcgcttcc ccgccccttc gcgtaggatt
    19321 cccgcgaagg ggcgttgtcg tgaaggccct tccatcaccc cggaggcgat cgtgaagatc
    19381 ctggtcggcg tcgacgagtc cccgttctcg caggcggccc tcgattgggt gaagcagatc
    19441 ccgtggccat cgggcacgag catccgcgtg gtctcggcgt ctccccctgt gttcgtcggg
    19501 acgggcgaag ccaatgcgcc ggccgtcgtc gcgcagctca tccaggacca gaaccgcatg
    19561 cacgcggaga tcgccggccg cgcggcccga tcgctcgagg catcgggacg gacggtggag
    19621 cccgtcatga tctccggaga cccgcgctcc acgctggtcg aggaagcgcg caagatcggc
    19681 gccgacctcg tggtcgtcgg ctcgcacggt cggagcggtc tctcgaggct cgtcctcggg
    19741 agcgtcgcgt cgtacgtgtc gtcgcacgct ccgtgcagcg tgctgatcgt gaagcggccc
    19801 cacgacgccc ggtgatcccc gcccagggct gaccccaagc cgatccgcac gctataatcc
    19861 gccgcatgcg tgatcggatc ggctcgtttc gaatcctcga acgcctgggc gagggtggga
    19921 tgggggtggt gtacgcggcc caggacgagc gcctgcagcg ccgcgtcgcc atcaagatga
    19981 tccgggatga ctccgggctc cgcgaggccc gcgaccgctt ctggcgcgag gcgcgctccg
    20041 cggcgaagct caaccatccg aacgtctgcc agatctacga cgtgggcgag gaaggctccg
    20101 atctctggat cgcgatggag cttctcgagg gagagccgct cgcctcgcgc atggcgcggg
    20161 atcccatctc cctgaacgag gcgggcccga tcgtcctcgg gatactcgcc gccctcgacg
    20221 cgctccaccg ccaccagatc gtgcaccgcg atctcaagcc gtcgaacgtc ttcctcacct
    20281 cgcacggggt gaagctcctc gacttcgggc tcgcacgccc catcgaggac tcggctcagg
    20341 tcaccaccac gaacctgacc cggaccggga tcgtcatagg gacgccgcgc tacatgtcgc
    20401 cggagcagtg gaagcgggag ccggtcgacg cgaggagcga catcttcgcg gccggggtca
    20461 tcctctacga gatgatctcc ggcgcgcccg cgttccccgg gcagtccccg ggcgagatcc
    20521 ggcacgcggc gctcttcgac cagccccccg ctctctccgg cccaccggcg gtcatggcgg
    20581 tggaccgcgt gatccagcgt gccatctcga agggctccgc cgaccgccat ccgaccgccc
    20641 aggccatggc ggacgagctg cgcagcgcgc tccgcctcct cgaagcgggc gacgctccgc
    20701 gcgtgcgcgc ggtgacgcga ctcatcgtgc tccccttccg cgcgctccgc cccgatcccg
    20761 actgcgactt cctcacgttc agcctgcccg acgcgatcac gggctcgctc gcggggatcg
    20821 actcgctcgt ggtgcgatcg agcctcacgg cggccaggtt cacgagcgcg acgcccgatt
    20881 tgaagcagat cgcggaggag accgaggtcg accttgtcct gacgggaacc ctcatgcgcg
    20941 ccggcgacaa ggttcgcgtc aacacgcagc tcctggacgc cgtcacggga acggtggctt
    21001 ggaccctctc gacgcaggcc tcgatgggcg atctgttcga gctgcaggac ggcctcgcgc
    21061 ggcacatcgt cgaatcgctc tcgctgccgc tctcggcacg cgagcggacc tcgctccaga
    21121 aggacgtgcc ggtgagcgcg aaggcctacg agttctatct gcgcgccaac cagctcgcct
    21181 ggacctcgga cgggtggacc ctcgcgcgcg atctctacct caagtgcctc gaggcggacc
    21241 ccaagtacgc ccccgcgtgg gcccagctcg gccgcatcta ccgggtgatg gcgaagtacc
    21301 tcgaggacaa tcccaaggag aacctgctcc gcgcggccga cgcgttccac cgctccctcg
    21361 agatcaaccc ggatctctcg ctcgcgcaca atctctacgt gtacctcgag gtcgagatgg
    21421 ggcgcgcgac cgaagcgatg acgcgcctcc tcgaccgcgc gcgggaccgt ccgtcggagg
    21481 cggggctgtt cgccgggctc gtccaggcct gccggtattg cggcctcctc gacgcgtcgc
    21541 tcgcggcggc cgagcgcgcg ctgaggctcg atcccaacat cccgaccagc atcacgtaca
    21601 cgcactggat gtcgggggac tacgagcgcg ccatgcagct cagggcgaac gatctcccgt
    21661 tcatccgggg ctacagcctg atgtcgctgg ggcggcgcga ggaggcgctg gaggtgttcg
    21721 cccagatgaa ggacatgatc cacctccccg gattcggccg ctccatgtat cacctgctcg
    21781 tcggaaaccg cgagggctcc ctctccgaca tacgctcggt cttgaagtcg gcgttcctcg
    21841 accccgaggg gatctacttc atcgcgcgag ggctcgccta ctggggcgag ccggaggagg
    21901 ccctccgcac gctggatcga gtcgtggagg gagggttcta ctgcgaccag acgatggcgc
    21961 gggacccgtg gctcgattcc atccgcacca caccggagtt cgtccggatc ctgcgccgcg
    22021 ccgagtcgct gcgccggagt gcggtcgccg cgtacctgga gcaccagggc gaccgcatcc
    22081 tcggcgttcg ccccgccaac taggctgtcg ccgcttcctc cggcggccgg tgccgccgga
    22141 acgccgcgta gagcaggaag tcgtaggcga tcttcatccc cgctccgatc caaagcggca
    22201 ccgcgtacga ccctccgcgc atgaagagcc ccgcgaacgc gggcgccgcc gcccacgcgc
    22261 cgagcctcac gagatgcgtg attccggagg cgagcgttcg ctcctcgggc ttcaccaccg
    22321 ccatcacgta cgactgccgc gtcggcacgt ccatctcgac cagcccctcc cggaggagga
    22381 agagccccgc cgcgaccggg aagctcggcg cgaacggcac ggtggcgagg agcaggctcg
    22441 atgggacgtg ggtgaagacc atcgtgttca cgagcccgat cctcttggcc agccaggccg
    22501 cgcccatgtg cgagagcgcg ttcatggcgc gcgcgccgaa gaagagaact cccaccaccg
    22561 cctcgctcac atggaagcgc tcgaagaaga agagcgagag gagtgcggtc gtcaggaatc
    22621 cgccccccag actgtcgagc gtgaagagcg ccgaaatgcg cgtgacgatc ttccgcgtct
    22681 ccggggagag acgggcgcgc gccgtgggcg tcctggactc cacggcgggc gagagggcgc
    22741 ggtagaggat cgcggtcacg aggaggagca ccgagtagag cccgaccgcc atgcggagcg
    22801 acgcgatctc ccccaccggc gcgagcgccc ggatcgcggc cggcaccgcg gcgaggagcg
    22861 cccccagagc gtgccccgcg tcctggacca cgttgtatgc ggcgaagacc gtggtgcggc
    22921 ggtcgttggt cgtggtggtg ggaaggatga cctggtccag gatcatcgag gcgccgcggt
    22981 cccgccccat gccgttcagc atgccgagga acgccgccac tgcgacaagc gcgttcttcg
    23041 gcgcgaggag gaccaggact ccgccggccg ccccgagcac gccgagcgcg gtcagcacgc
    23101 gcctgcggcc cacgcgatcc cccgccagcg tcacgaggag cgctgcgagc gccgccccgg
    23161 cgagtccggc cgcgaccacc gcgccgatgg cggccggatc gaagccgacc cgggcgagat
    23221 agatcgcgat caggaccgcg atcatgccgg tcgcgagcgc tcgcaggaag gcgcccgcat
    23281 agaggatcag gcggtcgcgc cgtccggcag tgggagggag gcccgactcg gcggtcatgg
    23341 gtccgccaga cgcggcagcg tgtgcgtcag ccccccagtc tggacgtgat gtagaacgtc
    23401 agcgccgccc cgaacgccga ggcggggatc gtgagcaccc acgcccacac gatcctcccc
    23461 gcgacacccc accgcaccgc gctcaggcga tgggtcgcgc cgacaccgac gatggcgccc
    23521 gtgatggtgt gcgtcgtcga caccgggatg cccaggtggg tggccccgaa gaggctgatg
    23581 gcgcccgccg tctcggcgca gaagcccccc accggccgga gcttcgtgac ccgcgcgccg
    23641 agcgtgtgca cgatccgcca cccgcctgtg agcgtgccga gcccgatcgc gatgccgcag
    23701 acgatcacca cccattgcgg gatgaagaag tgatcgatct gccccgtcgt gaagaggagg
    23761 ag
//
LOCUS       DASTKC010000136         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1758733, whole genome shotgun sequence.
ACCESSION   DASTKC010000136 DASTKC010000000
VERSION     DASTKC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758733"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFR25_05575"
     CDS             complement(<1..407)
                     /locus_tag="VFR25_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940549.1"
                     /translation="MTDRHGIGDHATLALELPAVLGAIAGRARSQPGRERILDLAPHR
                     RIDHAIAAQSIYADLMACADQGDAPPTVAPPDLRPELERLSREGAALRGDELWRLSVL
                     LEQAGQVVTWHRKTRRETPGLDRMLAGVDPVEGL"
     gene            complement(570..761)
                     /gene="rpsU"
                     /locus_tag="VFR25_05580"
     CDS             complement(570..761)
                     /gene="rpsU"
                     /locus_tag="VFR25_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET9940550.1"
                     /translation="MPGIRVKEGESFESALRRFKKKCEKAGVLADLRRHQHFEKPSER
                     RKRKLNAAKRKMMARMKAD"
     gene            complement(872..1618)
                     /locus_tag="VFR25_05585"
     CDS             complement(872..1618)
                     /locus_tag="VFR25_05585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET9940551.1"
                     /translation="MPDTRRPDTTFWVDPRLVRSDRLTLNTEESHHLVRVHRASEGTP
                     FTAVDGTGVAYDCEVESIDGDGVTAKITARQPGRGELPFAVHVLVGLPDMGPAEAVME
                     HVVPLGASRIDFVACARSGRSPLGPNKIERLLRIARAGLKQSRRCKSTAIVSSASLAE
                     AVGALPPGLRVVADPWGGPIDAFQGPYPAISLAVGPPGGFDPEEYALLAKAGFQPISL
                     GPSRLTTETATISLLAVARNRLLPKGLGQI"
     gene            complement(1628..>2605)
                     /locus_tag="VFR25_05590"
     CDS             complement(1628..>2605)
                     /locus_tag="VFR25_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET9940552.1"
                     /translation="DTDKRAKYDRFGHAAEAPFGGAGGQDFAGFDLADALRAFMRDFG
                     GAGGMGGFGDVFEEGGRGGRPRERRGNTLEIRLPLTLEEIATGTEKVVKIRHMRVCGT
                     CKGSGAKPGTEKKTCPVCHGSGQVRLVQRSIFGQMINITTCDRCHGEGQIVESPCDTC
                     HGEGRVRQQSEISIKVPAGVSNGNYIPISGMGDAGPRGGPPGDLIAHIEELEHELFLR
                     DGDDLVIEVPISYSRAALGGKVEVPVLGGGRAMVEVPAGTTYGKFLRLRGKGLKSLKR
                     SGHGDLLARIVIPTPNKVSDRAKKVLQELEQLPEAKVGAARRPSEDVLP"
BASE COUNT          407 a          885 c          873 g          440 t
ORIGIN      
        1 agcccttcga cgggatccac cccggcgagc atgcggtcga ggccgggcgt ctcgcggcgc
       61 gtcttgcggt gccacgtgac gacctgcccc gcctgctcga ggaggacgct cagccgccag
      121 agctcgtctc cgcggagcgc cgcgccctcg cggctcagcc gctcgagctc ggggcggaga
      181 tcgggaggtg ccacggtggg cggagcatcc ccctgatcgg cgcaggccat gaggtccgcg
      241 tagatcgact gcgcggcgat cgcgtgatcg atgcgacggt gtggagcgag atcgaggatc
      301 cgctcgcggc cgggctggga acgcgcccgc ccggcgatcg cgccgagcac ggcgggcagt
      361 tccaatgcca acgtcgcgtg atccccaatg ccgtgtcgat cggtcatcgg agcatccgct
      421 ctggaacgcg tggtcgcgtc gctaatggcg gtagtatccg gactgagggt gaacgtgact
      481 tgtgggacag ccgaagtacc gactcaggag gcaaacggga aaccggcccg acgccggtct
      541 cccgcccgtt actgcgctgc cgaatgggcc tagtcggcct tcatccgggc catcatcttg
      601 cgtttcgcgg cgttcagctt ccgcttgcgc cgctcgctcg gtttctcgaa gtgctggtga
      661 cgacgcagat cggcgagaac tcctgccttc tcgcacttct tcttaaaccg gcgaagggcg
      721 ctctcgaagg actcgccttc tttgaccctg atgccgggca tctaaggaat cgcctcctaa
      781 ccatggtttg aggtaagcgg gacaagcctc ccagtaagtg ttgggcagca tccggctaac
      841 actaggaaga taccccgcat acgcgatttt gtcaaatttg acctagaccc ttggggagca
      901 agcgattgcg cgccacggcc aaaagcgaga tcgtcgcggt ttccgtggtc aaccggctgg
      961 gacctaagga gataggctga aaaccggcct tcgcgagcag ggcgtactcc tcgggatcga
     1021 atccgccggg aggcccaacc gcaagcgaaa ttgccggata gggcccttgg aaggcgtcga
     1081 tggggccacc ccaggggtcg gccaccaccc ggaggcccgg cggaagcgcg ccaacggcct
     1141 cggccagcga ggcgctggag acgatcgcgg tcgacttgca cctccgcgat tgcttcaggc
     1201 ccgcgcgcgc gatccgcagg aggcgctcga tcttgttggg cccgagcggg gatcgcccgc
     1261 tgcgcgcaca ggccacgaaa tcgatgcgac tggcgccgag cggcacgacg tgctccatga
     1321 cggcctcggc tggacccatg tccggaagcc cgaccaggac atggacggcg aatgggagct
     1381 cgcctcggcc gggctggcgt gccgtgatct tcgcggtcac gccgtccccg tcgatcgatt
     1441 ccacctcgca gtcgtaggcg acgcccgtgc cgtcgacggc cgtgaaggga gtgccttcgc
     1501 tcgcgcggtg cacgcggact aagtggtggg actcttcggt gttcagggtg aggcggtcgc
     1561 tgcgaaccaa cctgggatcg acccagaagg tcgtgtcggg acggcgggtg tcaggcatgc
     1621 acccagtcta cggcaagacg tcctcggacg ggcggcgcgc tgctcctacc ttcgcttccg
     1681 gaagctgctc caattcctgc aggaccttct tcgcgcgatc ggagaccttg ttcggcgtcg
     1741 ggatcacgat gcgcgcgagg aggtctccat gtccggagcg cttcaggctc ttcaatccct
     1801 tgccccggag gcggagaaac ttgccgtacg tggtgcccgc ggggacttcg accatcgccc
     1861 ggccgccgcc cagaacgggc acctcgacct tgccgccgag cgccgcgcgc gagtagctga
     1921 tcggcacctc gatgacgaga tcgtcgccgt ccctgaggaa gagctcgtgc tcgagctcct
     1981 cgatgtgcgc gatcagatcg cccggaggtc cgccgcgcgg ccccgcgtcc cccatgccgg
     2041 agatcgggat gtagtttccg ttcgagacgc cggccggaac cttgatcgag atctcgctct
     2101 gctggcgcac ccgcccctcg ccgtggcacg tgtcgcacgg cgactcgacg atctgccctt
     2161 ccccgtgaca gcggtcgcag gtcgtgatgt tgatcatctg cccgaagatc gagcgctgca
     2221 ccaggcgcac ctggccggag ccatggcaga ccgggcaggt cttcttctcg gtgcccggct
     2281 tggcgcccga tcccttgcac gtgccgcaca cgcgcatgtg gcggatcttg acgaccttct
     2341 ccgtgccggt cgcgatctct tccagcgtga gcggaagccg gatctcgagc gtgttgccgc
     2401 ggcgctctcg cggacggccg ccccggccgc cttcctcgaa gacgtcgccg aatccgccca
     2461 tcccgccggc gccgccgaag tcgcgcatga acgcgcgcag cgcgtccgcg agatcgaagc
     2521 cagcgaagtc ctgtccgccc gcgccgccga acggcgcctc cgcggcgtgt ccgaagcggt
     2581 cgtactttgc gcgcttgtcg gtatc
//
LOCUS       DASTKC010000137         5702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1996331, whole genome shotgun sequence.
ACCESSION   DASTKC010000137 DASTKC010000000
VERSION     DASTKC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996331"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2174)
                     /locus_tag="VFR25_05595"
     CDS             complement(<1..2174)
                     /locus_tag="VFR25_05595"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET9940553.1"
                     /translation="MSLRRIRFGHAAVLVVLLLVAAVQPALAGSASKPVPPAPASAPS
                     IYQETLPNGLRLVLVEDHSKPLAGVCIFVNGGSRTESPALSGLSHYYEHLIFRGGSAR
                     QQELEFRREMQRLGEESGGYTTNDYTCYGFTSPTSNLDEALWRSVDAWMALKLTEPKV
                     AKERQVVMEEFNQGEDRPDYKVYYQIERLMFRDHPYKRDTIGLKDTIENSKLSTFRTF
                     YEERYVPNQMVLAAVGDFNTQAMAGKLRKAFAPYARGKDNFELGQIEAPQAEFRMGVE
                     SMKTPSTWTYLGFHTPPNADSDTPALTVLASLLGKGTSSRLYRALKDKENLVQTVDAD
                     LEIRRDPGMFLISTQAPPENEARVFGIVRDELHRVATEPVTAAELTRVKAAILNKYAL
                     NAQTFFARAERLCLNELMADATMDASFPKLIESVTADDLRRVASRYLAADLASYSVVR
                     PEGTKGPSEQDVDLMLAAWRSGWPALASAKTAGVGAVRREVLSNGLTLLVQEDHATPI
                     VAVQAFARGGQWIEPDGLAGVSNMAAALLQRGAGSLSAHDIAEQTEALGMTLTSAGYS
                     DDAAISWQAPSANFGKAWSIFESVLAHPRFPSSEVAKVREDLIRSAKSIGDRPFEFTN
                     LRFAQTLYRRSPYRFPLQGDETTLPKIQTSDLKKAYETMFCGSNLVLSVVGDVDANRV
                     VEMARHSFGSLRKGSPVTVGGVVEDAAKEKRPVFVDKDQEQVT"
     gene            complement(2248..2469)
                     /locus_tag="VFR25_05600"
     CDS             complement(2248..2469)
                     /locus_tag="VFR25_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2437..2439),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HET9940554.1"
                     /translation="MNVEIRYCVLUNYKPRAAGLAAELMQEMGAESTLTPGGGGVFDV
                     IVDGKTVFSKKVIGRFPEAGEIVSMLQKR"
     gene            complement(2528..2968)
                     /locus_tag="VFR25_05605"
     CDS             complement(2528..2968)
                     /locus_tag="VFR25_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9940555.1"
                     /translation="MRSIFSTTPTYWQLPLRLCLFIVFWMHGSQKVLGMYGGPGLSGF
                     VGWLTSTGVPAPLAYLAAFAEFLGCFGILLGFLTRIAAFGIACNMTVAFLTVHLKNGY
                     FGGPNGPGYELVMILWFALVTLLIGGGGNLSVDRAIYLKRRTSP"
     gene            complement(3067..>5702)
                     /locus_tag="VFR25_05610"
     CDS             complement(3067..>5702)
                     /locus_tag="VFR25_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9940556.1"
                     /translation="DRFWVGSTGVLEEVRGGDNEIPLFASKTSKDSVTVSLRVAMADT
                     LAGHWPAKGLEITTDLAAPKGSDPPKLVVPKGGALSFRVPRGSPVHYSYIWEEREPRD
                     GDDAKGDHATRSEADEAGRGGPLHLVTATRDTTLIDTLRALARVAAPTDTLPPSEKTL
                     RFHADHVHDFAWVASPEYVRSDSTHAGIAIRTLAFREDEKDWRVVNETVARAMTLYSH
                     LAGPYIWPQFTYSEAFCGGGAMEYNMLVMAEPDFKTTYFRWMENTVAHELAHNWFYGM
                     IGTDERAFPWLDEGFAQYFDHRYTDETFPKGIFVYNDRIPWLSGYTMRSRNERDYLEG
                     AWAHDERTLSTPANGHTGYDTYGSSAYSKPASMLHTLRGVLGDSVFTAFLREYYKRGV
                     LKHPMPEDVRAAAEAASGQDLSNFFHSWVETLEQPSFGLSKEGSKRSGDEYSTRLVVK
                     RKGEMVYPLTVQARFKDGSVEEKRVVPDGPSTTVEFTSKSPLKGATLDPRHQAIETDR
                     LDNHVGGFWQDPIRVRPFFGFPSSDAIGVDAGPTLWYGTAEGVRVGGWLDGRYLPSTD
                     FPYGIRGFEAGLNIGTNDGSVAYRVGSWSRWGALGARSRLRWLVADDEGLFRTLVSAE
                     NLITGSARRYPHKSWRLAVEYRSRHDLEPVDPRYWSEGETIEPRLELGLDTRGPRRIE
                     HYHLAYGHGFAADGGDDFNRGEFSVRQDLDLLPAGDLHVGWRGLAGGASGDVPSEALL
                     DVAEDARLEALRYFYANDLGPIRNTDHYFLPGGGGLRGYADRAILGDHLLAFNLDVQH
                     DAYPVRAFAGVARVRGYVPDFLLDLFPRETRTYSEAGLGFSYGPVQLDFPFWLGTPDP
                     GESPWKVRWRFTFLPIKLPRL"
BASE COUNT          994 a         1860 c         1906 g          942 t
ORIGIN      
        1 gtgacctgct cctgatcctt gtcgacgaaa acggggcgct tctcctttgc cgcatcctcg
       61 acgacgccgc caactgtcac cgggctcccc ttccggagcg agccgaagga gtggcgcgcc
      121 atctcgacca cccggttcgc gtcgacgtca ccgacgaccg agagtacgag gttcgatcca
      181 cagaacatcg tctcgtacgc cttcttgaga tcggaggtct ggatcttggg cagcgtggtc
      241 tcgtcaccct ggagcgggaa gcggtacggc gagcggcgat agagcgtctg cgcgaagcgg
      301 aggttggtga actcgaacgg ccggtcgcca atcgacttcg cgctccggat cagatcctca
      361 cgaaccttcg ccacctcgct cgaggggaac ctggggtggg ccagcacgct ctcgaaaatg
      421 gaccaggcct taccgaaatt cgccgacggc gcctgccacg agatcgcggc gtcgtccgag
      481 tagcccgcgg acgtcagcgt catgcccagc gcctcggtct gctccgcgat gtcgtgcgcg
      541 gagagcgagc ccgcgccgcg ctgaaggagc gcggccgcca tgttcgacac gccggccaga
      601 ccgtccggct cgatccactg cccgccgcgc gcgaacgcct gcacggcgac gatcggcgtc
      661 gcgtggtctt cctgcacgag cagggtcaat ccgttcgaga gcacttcgcg gcgcaccgca
      721 ccgacaccgg cggtcttggc ggatgcgagc gccggccatc cggaacgcca tgccgcgagc
      781 atgagatcga cgtcctgctc ggacggacct ttcgtaccct cgggccgcac caccgaatag
      841 gacgcgagat ccgcggcgag gtagcgcgac gcgacgcgac ggagatcatc ggccgtcacg
      901 ctctcgataa gcttcgggaa cgaagcgtcc atggtcgcat cggccatgag ctcgttcaga
      961 cagagccgct ccgcgcgggc gaagaaagtc tgcgcgttca gcgcgtactt gttgaggatc
     1021 gcggccttga cgcgcgtgag ctcggccgcg gtgacgggct ccgtggccac gcggtggagc
     1081 tcgtcccgaa cgatgccgaa cacccgtgcc tcgttctcgg gcggcgcctg cgtcgagatc
     1141 aggaacatcc ccggatcgcg gcggatctcg aggtcggcgt cgaccgtctg gaccaggttc
     1201 tccttgtcct tgagcgcccg ataaagacgc gaggacgtcc ccttgccgag cagcgacgcg
     1261 aggaccgtga gcgcgggcgt gtccgagtcg gcgttcggcg gcgtgtggaa tccgaggtac
     1321 gtccacgtac tcggcgtctt catggactcg acgcccatcc ggaactcggc ctgcggcgcc
     1381 tcgatctgcc ccagctcgaa gttgtccttg ccgcgcgcgt acggggcgaa cgccttccgg
     1441 agctttccgg ccatcgcctg ggtgttgaag tcccccaccg cggccaggac catctggttc
     1501 gggacgtagc gctcttcata gaacgtgcgg aaggtcgaga gcttggagtt ctcgatcgtg
     1561 tccttcagcc cgatcgtgtc ccgcttgtag ggatggtcgc ggaacatgag ccgctcgatc
     1621 tggtagtaga ccttgtagtc gggccggtcc tcgccctggt tgaactcctc catcacgacc
     1681 tgtcgctcct tcgcgacctt cggctcggtc agcttgagcg ccatccacgc gtccaccgaa
     1741 cgccagagcg cctcgtcgag attcgaggtg ggggacgtga atccatagca ggtgtagtcg
     1801 ttggtcgtgt atccgccgct ctcctcgccc agccgctgca tctcgcgtcg gaactcgagc
     1861 tcctgctgcc gcgccgagcc gccgcggaag atcagatgct cgtagtagtg gctgagcccc
     1921 gagagcgcag ggctctcggt gcggctcccg ccgttcacga agatgcagac cccggcgagc
     1981 ggcttcgagt ggtcctcgac gaggacgagg cgaaggccgt tcgggagggt ttcctggtag
     2041 atcgagggag cgctcgcggg cgccggcggg accggcttcg acgcggaccc cgccagggcc
     2101 ggctggacgg cggcaacgag gagcagcacg accagcaccg cggcgtggcc gaatcggatg
     2161 cgacgcaagc tcatgatttt ccccctcgaa acaggaccgg ccccgcggca agcggggccg
     2221 gcggaatgga accgctgtcc gttctgatca gcgtttctgg agcatcgaaa cgatctcgcc
     2281 ggcttccgga aaccggccga tcactttctt ggaaaagacc gtcttgccgt ccacgatgac
     2341 gtcgaacacg ccaccgccgc caggcgtcag cgtcgattca gcgcccatct cctgcatcag
     2401 ctcggccgcc agaccggcgg cccgtggctt gtagtttcaa aggacgcagt agcggatctc
     2461 gacattcatg acgtaccccc tgtgggtggc ttggacgcgg acattctctg cccgggtcgg
     2521 ggggctgtca aggcgaggtc cgtcgtttca agtagatggc ccgatcgacg gaaaggttgc
     2581 cgccgccgcc gatcaggagc gtgaccaggg cgaaccagag gatcatcacg agctcgtagc
     2641 ccggaccgtt gggcccgccg aagtagccgt tcttgaggtg gacggtcaga aacgccacgg
     2701 tcatgttgca tgcgatcccg aacgcggcga tgcgcgtgag gaatccgagt aagatcccga
     2761 agcagccgag gaactcggcg aaggcggcga gatacgcgag cggcgcgggt acgcccgtgc
     2821 tcgtcagcca tccgacgaac ccggagagcc cggggccgcc gtacatgccg aggaccttct
     2881 gcgagccgtg catccagaag acgatgaaga gacagaggcg aagcgggagc tgccagtagg
     2941 tcggtgtggt cgagaagatc gaacgcattc gactgcacct ccatagaggt ggtgaagcgc
     3001 cggacaccgg tcgagccggc gggcgagagg ccgtcgatca gcctgacaca gccgggccgt
     3061 ccgtgactac aggcgcggaa gcttgatcgg caggaacgtg aagcgccagc gcaccttcca
     3121 cgggctctcg ccggggtcgg gagtgccgag ccagaacggg aagtcgagct ggacggggcc
     3181 gtaggaaaag ccgagcccgg cttcggagta ggtgcgggtc tcgcgcggaa agagatcgag
     3241 aagaaagtcg ggtacataac cgcgcacgcg ggccactccg gcgaaagccc gaaccggata
     3301 ggcgtcgtgc tggacgtcca gattgaaggc caggaggtgg tcgccgagga tggcgcggtc
     3361 ggcgtagccg cgcaggcccc caccgccggg caggaagtag tgatcggtgt tccggatcgg
     3421 gccgaggtca ttggcgtaga aatagcggag tgcctcgagg cgggcgtcct cggcgacgtc
     3481 gaggagagcc tcactcggga cgtctccgct ggccccgcct gccaaccctc gccagcccac
     3541 atggagatcg cccgcgggca gtagatccag atcctgccga actgagaatt ccccccggtt
     3601 gaagtcgtcc ccaccgtccg cggcgaagcc gtgtccgtag gccaagtggt agtgctcgat
     3661 gcggcgcggg ccgcgcgtgt cgaggccgag ctctagacgc ggctcgatcg tctccccttc
     3721 ggaccagtac cgtggatcca cgggctcgag atcgtggcga cttcggtact cgaccgcgag
     3781 gcgccaggac ttatgcggat agcgccgggc cgagcccgtg atgagattct ccgcgctcac
     3841 gagcgtgcgg aagagaccct cgtcgtccgc aacgagccag cggagccgac tccgcgcgcc
     3901 gagcgcgccc cagcggctcc acgagcccac gcgataggcc acggagccgt cgttggtgcc
     3961 gatgttgagc ccagcctcga atccgcggat cccatagggg aagtccgtcg acggcaggta
     4021 gcgaccgtcg agccagccac cgacccgcac cccttccgcc gtgccatacc agagcgtcgg
     4081 gccagcgtcg acgccgatgg catcggatga cgggaacccg aagaagggac ggacgcgaat
     4141 cggatcctgc caaaatccgc ccacatggtt gtcgagccgg tccgtctcga tcgcttgatg
     4201 acgcggatcc agcgtggcgc ccttgagcgg agacttgctc gtgaactcga cggtggtgct
     4261 cgggccgtca ggaaccaccc gcttctcttc gacggagccg tccttgaacc gagcctggac
     4321 cgtgagtgga tacaccatct cgcccttccg tttcacgacg agacgagtgg agtattcgtc
     4381 cccggagcgc ttggacccct ccttcgagag cccaaagctc ggctgctcga gcgtctccac
     4441 ccatgaatgg aagaagttcg agagatcctg ccccgatgca gcctccgctg cggctcggac
     4501 atcctcgggc atggggtgct tcagcacgcc gcgcttgtag tactcccgca ggaacgcagt
     4561 gaagaccgaa tcgcccagaa ccccgcgcag cgtgtggagc atgctcgcgg gcttcgagta
     4621 agcgctcgag ccgtacgtgt cgtagccggt gtggccgttc gcgggcgtgg agagcgtccg
     4681 ttcgtcgtgc gcccaagcac cctccaggta gtcgcgctcg ttgcgcgagc gcatcgtgta
     4741 cccggagagc cacgggatcc ggtcgttgta aacgaagatt cccttgggga aggtctcgtc
     4801 cgtgtagcga tggtcgaaat actgcgcaaa cccctcgtcc agccacggga acgcgcgctc
     4861 gtcggtgccg atcattccgt agaaccagtt gtgcgccagc tcgtgggcta ccgtgttttc
     4921 catccaacgg aagtacgtcg tcttgaagtc cggctccgcc atgacgagca tgttgtattc
     4981 catcgcaccg ccgccgcaaa acgcctcgga gtaggtgaac tggggccaaa tgtaagggcc
     5041 cgcgagatgc gagtagagcg tcatcgcccg cgccacggtc tcgttgacga cgcgccagtc
     5101 cttctcgtcc tcgcgaaacg cgagcgtacg gatggcgatc ccagcatggg tggaatcgct
     5161 ccggacgtat tcgggggagg ccacccacgc gaagtcgtgg acgtgatcag cgtggaagcg
     5221 aagcgtcttc tcagatggtg gcagcgtgtc cgtcggggcc gcgacgcgag cgagcgcacg
     5281 cagggtgtcg atgagcgtcg tgtcgcgcgt agcggtcacc aggtggagcg gcccgccgcg
     5341 cccggcttcg tcggcctcgc ttcgcgttgc atgatccccc ttcgcatcgt caccgtcgcg
     5401 cggctcacgt tcctcccaga tgtacgagta gtgaaccggt gaccccctgg gtacgcggaa
     5461 agagagcgcc ccacccttcg gcaccacgag cttcggcgga tccgaaccct tgggcgctgc
     5521 gagatccgtc gtgatctcga gccctttggc cggccagtgt cccgcgagcg tgtccgccat
     5581 ggcgacgcgc agcgaaaccg tgacgctgtc cttggaagtc ttcgatgcga agagcgggat
     5641 ctcgttgtcg ccgccgcgaa cttcctcgag cacgcctgtg gaccctaccc agaaccggtc
     5701 cg
//
LOCUS       DASTKC010000138         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1050739, whole genome shotgun sequence.
ACCESSION   DASTKC010000138 DASTKC010000000
VERSION     DASTKC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050739"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VFR25_05615"
     CDS             complement(<1..1023)
                     /locus_tag="VFR25_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HET9940557.1"
                     /translation="MLRRQATLAPVLVALLVGTPFAAIAAPSKATPVVAPWGVSLDYI
                     DSTVHPGADFFTYVNNGWLKTAQIPPDRSAAGTFLDLIMRNDDRLKAIFVELHGRTDL
                     TEEEKKLRDLYEAYMDTTQIESSKLEPAAADLARINAAASLEDVARIQGDPSLRLEGP
                     FDMRIQVDDKDPDAYVVRLTQSGLGLPDRDYYLKDDAPLDTARRAYRDYLARMLELAG
                     RKDAPTRAAAVFALEKQIATAHWPVADRRDADKTYNPMTIAKLSAAAPGYPWAAYLDA
                     AGIPARSSKGERVVNVGELSAFPKLARIFAATPVDVWRDYLVVQYLHAFADFLPREID
                     DLNFVMY"
     gene            1191..2084
                     /locus_tag="VFR25_05620"
     CDS             1191..2084
                     /locus_tag="VFR25_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9940558.1"
                     /translation="MRRIILLVLLLGGGIAIWKATHRREDYTGGDVQTTGTIEAVQVR
                     LGFQVGGRIADIPVTEGMRVQPGMIVATLDASDLQVQLSMAKAALASAQASVAQARAT
                     RDRAALEMSRAKTLLASGYATPQQMDTAATAYKVAVAQVQAAEAQVRQSQSQIAQSEL
                     QLTYAVLRTPEGGEVSERIHLPGEIVTSGTPVVAIAETDTVKVHAAVDETRVGAVRPG
                     DRALVKVYTFDKRIFPGVVTDIQPSGDFATRKDWGAQRRDIRTFDVTARVPNPDHLLK
                     DGMTAEVTLVVSAPVRVEAER"
     gene            2081..>3040
                     /locus_tag="VFR25_05625"
     CDS             2081..>3040
                     /locus_tag="VFR25_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9940559.1"
                     /translation="MIAPEGIPTSEPAIVVEHLTKRFGEFTADDDLTFTVYRREIFGI
                     LGPNGAGKSTLIRMLNTLLPPTSGRALVMGHDVVAKTNEVRGEIGVIPQANTVDGDLT
                     AMESLDLYARFYGMGRRERQERAKELLNAVGLWEWRDKIAGTYSGGMRRRLEIARGLI
                     HRPRVFFLDEPTTGLDPQSRRVIWELLERLREQGDLTILICTHYMDEADRLCDRLAIV
                     DHGKIAALGTPRDLKASVPGQDILTVTYQHDVDDALLSALKELPEIKEAAREDPHTAR
                     LILVGDTVPLDGITESSHKLGNKVRTLSLLEPTLEDVFIHYTGR"
BASE COUNT          516 a         1022 c         1054 g          448 t
ORIGIN      
        1 gtacatgacg aagttcaggt cgtcgatctc gcgaggaagg aagtcggcga aggcgtgcag
       61 gtactggacc acgaggtagt cgcgccacac gtcgaccggc gtcgcggcga agatccgcgc
      121 gagcttcgga aacgcgctca gctcgcccac gttgacgacc cgctccccct tcgacgagcg
      181 cgcgggaatc cccgccgcgt ccaggtacgc cgcccacgga tatccgggcg ccgccgccga
      241 gagcttcgcg atcgtcatcg gattgtaggt cttgtccgcg tcgcggcgat cggcgaccgg
      301 ccagtgcgcg gtcgcgatct gcttctcgag tgcgaacacg gccgcggcgc gagtgggcgc
      361 atccttccgc ccggcgagct cgagcatgcg cgcgagatag tccctgtagg ccctgcgcgc
      421 ggtgtcgagc ggggcgtcgt ccttcaggta gtaatcgcga tcggggagcc ccagtcccga
      481 ctgcgtgagc cggaccacgt acgcgtccgg atccttgtcg tcgacctgga tgcgcatgtc
      541 gaacggacct tcgagccgga gcgacgggtc gccctgaatc cgcgccacgt cctcgaggga
      601 cgccgccgcg ttgatgcgcg cgagatcggc ggccgccggc tcgagcttcg acgactcgat
      661 ctgcgtcgtg tccatgtagg cttcgtacag gtcgcggagc ttcttctctt cttcggtcag
      721 gtcggtccgg ccgtgcagct ccacgaagat cgccttgagc cgatcgtcgt tccgcatgat
      781 gaggtcgagg aaggtgcccg ccgccgaacg atccggcggg atctgggcgg tcttgagcca
      841 gccgttgttc acgtaggtga agaagtcggc gcccgggtgg acggtcgagt cgatgtagtc
      901 gagggatacg ccccaaggcg cgaccaccgg ggtggccttg gacggcgcgg cgatcgcggc
      961 gaagggcgtg cccacgagaa gagcgacgag gaccggagcg agggttgctt gacgccgcag
     1021 catggccgcc gaggctacca gggtccgccg cgcctggcaa cgccaggtag ccccaatcgt
     1081 gctgaatcgg tactctccct ggtccgatcc aagatcgaac aggtgcgagg cgggtgaccg
     1141 gaggaccacg ggtggagcag ccagcaaaac gtcccaatcg aggagcgatc gtgcggcgga
     1201 tcatcctcct ggtccttctc ctgggcggcg gaatcgcgat ctggaaggcc acccaccgcc
     1261 gcgaggacta cacgggcggc gacgtccaga cgaccgggac catcgaggcg gtccaggtgc
     1321 ggctcggatt tcaggtgggc gggaggatcg cggacatccc cgtgaccgag ggaatgcgcg
     1381 tccagccggg catgatcgtg gccacgctcg acgcgagcga cctccaggtg cagctctcga
     1441 tggcgaaagc cgcgctcgcc tccgcacagg cctcggtcgc gcaggcgcgt gcgacccgcg
     1501 accgcgccgc gctcgagatg tcgcgggcga agacattact tgcgagcggc tacgcgacgc
     1561 cgcagcagat ggacactgcc gcgaccgcgt acaaggtcgc ggtcgcgcag gtccaggccg
     1621 ccgaggccca ggtgcggcaa tcgcagagcc agatcgcgca gtcggagctc cagctcacct
     1681 acgccgtgtt gcgcacgccc gaggggggcg aggtctcgga gcgcatccat cttcccggcg
     1741 agatcgtgac ctcgggcacg ccggtcgtcg ccatcgccga gaccgacacc gtcaaggtgc
     1801 acgccgccgt ggacgagaca cgcgtcggtg ccgttcgccc gggcgaccgg gcgctcgtca
     1861 aggtgtacac gttcgacaag cggatctttc ctggcgtcgt gaccgatatc cagccctcgg
     1921 gcgacttcgc cacgcggaag gactggggcg cgcagcgccg ggacatccgc accttcgacg
     1981 tgaccgcccg cgtgccgaat cccgaccacc tgctcaagga cggcatgacc gcggaggtca
     2041 cgctggtcgt gagcgcgccg gtgagggttg aggcggagcg gtgatcgcgc ccgagggcat
     2101 cccgaccagc gagcccgcga tcgtcgtcga gcacctgacc aagcggttcg gcgagttcac
     2161 ggcggacgac gacctcacgt tcaccgtcta ccgccgggag atcttcggca tcctcggtcc
     2221 gaacggcgca ggcaagagca cgctcatccg aatgctcaac accctgctcc cgcccacctc
     2281 cggacgcgcg ctcgtgatgg gacacgacgt ggtcgccaag accaacgagg tgcgcggcga
     2341 gatcggcgtg atcccgcagg cgaacaccgt cgatggcgat ctcaccgcca tggaaagcct
     2401 cgatctctac gcccgcttct acggcatggg gcgccgcgag cgccaggagc gagccaagga
     2461 gctcctgaac gcggtgggcc tctgggagtg gcgcgacaag atcgcgggca cgtactcggg
     2521 ggggatgcgc cggcggctcg agatcgcgcg cggtctcatc catcggccgc gcgtcttctt
     2581 cctcgacgag cccacgacgg ggctcgatcc gcagagccgc cgcgtgatct gggagctcct
     2641 cgagcggttg cgggagcagg gggacctcac gatcctcatc tgcacccact acatggacga
     2701 ggcggatcgc ctctgcgatc gcctcgccat cgtggaccac gggaagatcg ccgcgctggg
     2761 gacgccgcgc gatctcaagg caagcgtgcc gggtcaggac atcctgacgg tgacgtacca
     2821 gcacgacgtg gacgacgcgt tgttgagtgc actcaaagaa ttgcccgaga tcaaggaggc
     2881 agcccgcgag gatccacaca ccgcgcgcct catcctcgtg ggtgacaccg tgccgctcga
     2941 cggcatcacc gagagctcgc acaagctcgg gaacaaggtc cggacgctct cgctgctcga
     3001 gccgacgctc gaggacgtct tcatccacta cacgggccgg
//
LOCUS       DASTKC010000139         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1760433, whole genome shotgun sequence.
ACCESSION   DASTKC010000139 DASTKC010000000
VERSION     DASTKC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760433"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1451
                     /gene="miaB"
                     /locus_tag="VFR25_05630"
     CDS             117..1451
                     /gene="miaB"
                     /locus_tag="VFR25_05630"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9940560.1"
                     /translation="MPDRIYIETYGCQMNVADSELMLGTLRAAGYERVERAGEADVIL
                     VNTCAIREHAEQRVYGRIGELSRYKVRRPGVVLGVAGCMAQHLKGKLMERAPYVDLVI
                     GPDGYRDLPGLVAQAREEPTLAVRLSRVETYGDLAPARADGVRAWVSIMRGCDKFCTF
                     CIVPYVRGRERSLPADEVIRQVEHAAAEGFKEIVFLGQTVNAYKEGDTDFAELLRRAN
                     AIDGIERIRFTSPHPADMTPRAIEAIATCDKVCPQVHLPLQSGSNPVLERMRRIYTIE
                     LYEELVGELRAAVPGLALTTDLIVGFPGETEEDFDKTHDVMRRLRYDSAFLFKYSARP
                     GAKAAEWPETVSEEEKTRRITVLIEEQKTISYERNLEMVGREVEVLVEGPAKRDPNQW
                     FGKTEHFKTAVFPHRDERVGDLIRLRVAAASPYTLFGAGASDVVRSMEDVTA"
     gene            1496..>2067
                     /locus_tag="VFR25_05635"
     CDS             1496..>2067
                     /locus_tag="VFR25_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940561.1"
                     /translation="MTSPASAVTPPGVSYRIDCRLDAARHRLTGWATLRYRSGADSSL
                     PSLYFHSYPNAFRDNHTVYAEEGERYAGDYGLRFSKKEDRGWMALDSCTVDGMPATVD
                     VQETLSRVDLPHPLAPGDSTEVRLRFTVQIPTHFDRLGRSGSRYSISQWYPKIVVYDK
                     RGWRLDPFHYFSEFYGEFGTYDVSLTLPDR"
BASE COUNT          347 a          689 c          668 g          363 t
ORIGIN      
        1 cgtatctgcg atcgctcgta acaccgccgg cctcggcccc gaacccatcc cagaactgac
       61 cggggccgcg gccccccgct cccgtccaag ccgcgtctgc cgtatacttt ccccccatgc
      121 ctgaccgcat ctacatcgag acctacggct gccagatgaa cgtggcggac tccgagctca
      181 tgctcggaac gctccgcgcc gcgggatacg agcgcgtgga acgcgcgggt gaggcggacg
      241 tcatcctcgt caacacctgc gccatccggg agcacgccga gcagcgggtc tatggccgta
      301 tcggcgagct ctcacgctac aaggtgcgac gtcccggcgt ggtgctcggc gtggcgggct
      361 gcatggccca gcatctcaag ggcaagctca tggagcgcgc gccttacgtg gatctcgtga
      421 tcggacccga tgggtaccgt gatcttcccg gcctcgtcgc gcaggcacgc gaagagccga
      481 ccctggccgt tcgcctgagc cgcgtcgaga cgtatggcga tcttgctccc gcgcgagccg
      541 acggcgttcg cgcgtgggtc agcatcatgc gcggctgcga caagttctgc accttctgca
      601 tcgtgccgta cgtgcgcggg cgcgagcgca gtcttcccgc ggatgaggtc atccggcagg
      661 tggagcacgc ggccgcggag ggcttcaagg agatcgtgtt cctgggccag accgtgaacg
      721 cctataagga aggcgacacg gacttcgccg agctcctccg gcgcgcgaat gccatcgacg
      781 gcatcgagcg gatccgtttc acctcgcccc atcccgccga catgacaccg cgagcgatcg
      841 aggcgatcgc gacgtgcgac aaggtctgtc ctcaagtgca cctgccgctc cagtcgggct
      901 ccaaccccgt cctcgagcgc atgcgccgca tctacacgat cgaactctac gaagagctcg
      961 tcggcgagct gcgtgctgcg gtgcctgggc ttgcgctcac gacggatctc atcgtcgggt
     1021 tccctggaga gaccgaggaa gacttcgaca agacgcacga cgtgatgcgg cggctccgct
     1081 atgacagcgc gtttctcttc aagtactcgg cccgtccggg cgcgaaggcg gcggagtggc
     1141 cggagaccgt ctcggaggag gagaagaccc ggcgcatcac cgtgctgatc gaggagcaga
     1201 agacgatctc ctacgagcgg aatctcgaga tggtggggcg cgaggtcgag gtgctggtcg
     1261 aagggccggc caagcgcgat cccaaccagt ggttcggcaa gacggagcac ttcaagacgg
     1321 cggtgtttcc gcatcgcgac gagagggtgg gcgacctgat ccggctgcgc gtcgcggcgg
     1381 cgtcaccgta tacgctgttc ggtgcggggg catcggacgt agtccgatcg atggaggacg
     1441 tcaccgcctg agtcgcgcgt cctgggcgct actcttcatc ctcatctcga gcggggtgac
     1501 cagccccgcg agcgccgtca ctccccccgg cgtttcctac cggatcgatt gccgtcttga
     1561 tgccgcgcgt caccggctca caggctgggc gacgctccgc tatcgaagcg gtgccgactc
     1621 gtcgctcccc tcgctctact tccattccta cccgaacgcg ttccgcgaca accacactgt
     1681 ctatgctgag gaaggtgagc gctacgccgg tgactacgga ctccggttct cgaagaagga
     1741 ggatcggggc tggatggcgc tggattcctg cacggtggac gggatgcccg cgaccgttga
     1801 cgtccaggag accctatcgc gtgtggatct gccgcatcca ctcgcgccgg gagattccac
     1861 ggaagtcagg ctccgtttca cagtgcagat cccgacgcac ttcgatcgtc tagggcgctc
     1921 cgggagccgc tactcgatct cgcagtggta cccgaagatc gtcgtctacg acaagcgcgg
     1981 gtggcgtctc gatccattcc actatttctc cgaattctac ggcgagttcg gaacgtatga
     2041 cgtctcgctc acgctgccgg accggtt
//
LOCUS       DASTKC010000140        12430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_578605, whole genome shotgun sequence.
ACCESSION   DASTKC010000140 DASTKC010000000
VERSION     DASTKC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12430
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578605"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VFR25_05640"
     CDS             complement(<1..1813)
                     /locus_tag="VFR25_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET9940562.1"
                     /translation="MCALITALLTAYTIAKVQRDATFIAEFGAGALPIAYVAVAIASA
                     AFVWVEGLFATRFSRLAANRINQYSAIAISLLAIPAFAAAPHWTAAAFYVWTGSQAMM
                     LLPHFWVLALDVWDSRRARRVFPLLSGFGLVGGLLGGAFAGWVAPILGRAALEWALAT
                     LLVVSFLLTRVVERHREERATSAPAAALAGSRWKIIRRSNYLQYLAAALALSVIVSTL
                     VDFQFKFFIQDIYPDTETLARFLGRFYLVLNLLALVFQFGAVGWLLTRIGLVASTGLQ
                     PALVMIFSWWVALSTGWWAVVALRWVQGVVLQGIGKSSAEIYYMAVRPDERRRIKPAI
                     DTLVERWSDAAVGVFLIIVMRVAGAALSAIAIATVVIAAGWLFVLYRLNRQYARAFEQ
                     ALSSRWLDAEGAAESMRVPGARRALEAALRSPDEPRTLLALDLARYIHHPRIAKAVLD
                     NLDRPSPAVRASTIRAMESLRLRDDRNRVRGMLEDADDRVCRAAVSYELAMSSRQVEY
                     ARSLLEGEDARLRGFMLDALLESPSEAPGAVTTAWVDRLMKSENADDRVLVARALGTV
                     TSSARVPRLLELLKEPSVEIRRAALQSAARRPSRELLD"
     gene            2230..2763
                     /locus_tag="VFR25_05645"
     CDS             2230..2763
                     /locus_tag="VFR25_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET9940563.1"
                     /translation="MSGFNRFAERLSGAVTEWAGGTAAFSMALLLIVGWVVLGPVFHF
                     SDTWQLVINTTTTIVTFLMVFLIQRAQNKDARATSLKLNELIASIEGASNRLVDVEDL
                     SEEELRTLHQHFRRLVEMSKRDASIMRSHSVEEAAVRHETKHAGHRPRGRRRRSSAQQ
                     ERGGAAKTNNNSKAQHS"
     gene            2760..3890
                     /locus_tag="VFR25_05650"
     CDS             2760..3890
                     /locus_tag="VFR25_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HET9940564.1"
                     /translation="MTIVERLQARGIRRLGSKERGFRYRTASGVAVTGEDRERIDALK
                     VPPAWRDVAIHPSPRAWVQAVGRDAAGRWQYRYHAKQVLRRERGKRKRLLRFIAALPR
                     MRRAVARDLELRGLKREKVLAGILRILSTCFLRPGSEEYASQNGSYGIATLKPRHVSV
                     RGDRIRFDFEGKSGRRQVRELRDRRLASLVRALLRHPGEVFKFESDSGAMVDVRREHI
                     NAYIMDVMGERFSSKDFRTWAGTLLCACALARDGGRKPSSRTEAKRRVAAAVREVAEH
                     LGNTPAVCRTSYVFPEVIRRFESGRVIADKIEGVDALVNGSPRALQRCERALLDLLTA
                     TSGTPRGSARRARPKPALRLEEAREPFGPTDHPEPRSVAIPR"
     gene            3832..5457
                     /locus_tag="VFR25_05655"
     CDS             3832..5457
                     /locus_tag="VFR25_05655"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9940565.1"
                     /translation="MSLSDRLIILNPGASRYPADEIRDVCETVCREHGLEGVFLESPT
                     GPGARASIRRVVREAVAGGCERIVAAGGDGTIGMVAEAVACAGAAHDVTLGIIPAGTA
                     NILARELGLQGSLADVAETAIVSDQSLAIDAIDVDGRLVFTQVGVGPDALMIRDTSRD
                     RQEKLGRLAYMITFAARALRHRPRRFTLSLDGSPIRALAWQVVAANVGTVGAPPFTWG
                     PRIDPTDGVLDLCVFDVRTLAGTLRLTWRILTGRHKRGASTMFYRVREEAVIDSPRPQ
                     LAQGDGEILGRTPITLRVVRDAVRVAVARAVNEADLAPPPAPGAAPSALPLAAARPEP
                     SLTEDVETMIAAHHARTWILQGVLRHPIAALEALDAALFLRLNRLTLGPIPDQVLTFA
                     SRYMHYGEGWILVLFAMMAQDLRHGLRVAVLVLPVLWLTLLTVNLPLKRFFRRRRPFL
                     AFVKARVLGPRPQDFSLPSGHAAAGFAGAFLLSLHAPAFAPLFYAVAIVVGLSRVYLG
                     VHYPSDVVIGAAAGTILAALYRWILLAVLPFRV"
     gene            5569..5970
                     /locus_tag="VFR25_05660"
     CDS             5569..5970
                     /locus_tag="VFR25_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940566.1"
                     /translation="MPVIVQVCVVIVTIAVVALVIAMVRMADRLSKLTEDIRTCLVEV
                     REVTGEVKSVAATAREVLKPVQRVVDRFERLGTRTAAVSSAFLEEVEGPVRTAVALVR
                     GVKTGAGFFMERLGSRFRPGRVATDGGYRYE"
     gene            5963..6223
                     /locus_tag="VFR25_05665"
     CDS             5963..6223
                     /locus_tag="VFR25_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9940567.1"
                     /translation="MNEYPYGSDLGQGSSNNMMGFFLGALVGASVALLLAPAPGGDTR
                     RRVADTAKKLGSAAKDKVAEMRGQAKEMGEMGRESYMQGRQG"
     gene            complement(6327..6788)
                     /locus_tag="VFR25_05670"
     CDS             complement(6327..6788)
                     /locus_tag="VFR25_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9940568.1"
                     /translation="MEVERIMTSDPACCTPDTPLQEVARLMVENDCGQIPVVDDRNNR
                     RTVGVITDRDIVCRALARGDNPLTMTAEHVMTRPVLTVRPSTKVEECCRLMEERQVRR
                     APVEDERGRCCGIVSLADIAQNLSEKTTGELTRTVSQPGSSASGTGRSMAL"
     gene            complement(6877..9675)
                     /locus_tag="VFR25_05675"
     CDS             complement(6877..9675)
                     /locus_tag="VFR25_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9940569.1"
                     /translation="MVAIAVVVLAAGFELRTAALEANILAKVASDMKYRVEPGPSDRI
                     VFPRGGPLDERRGYSRLPDFSKRLESRGFRIAAQARQSTALAMVSRFGIAPPYSEAPV
                     AGLVILGQDGKPIYDATAGRHGLARFEDVPPDVVRSLLYIENRELDREGSPYSNPAVD
                     WARFGKAMLLYSASRIGIPTPVEGGSTLAVQLEKYRHAEGGRTGSGVDKLRQMMSASL
                     RVYKDGPVTTADRRRIILDYVNTMPLAGAPGIGEIAGLDQGLSVWFGLDPKKVYKDLG
                     SKSATKRAKALKPVMALLCSVRAPSRYLIADHNALNDRVNAYAGLLAKEGVLEPKVAS
                     TVRKLPLKFASVPRTAPLPENPLGRGARRIRNELVSMLGLKDTYELDRLDLTVETTVD
                     EPLQVKVTQTLRQLADTSFVRARGLRGEHLLSQGDPKRVLYSVLLCERTPGGNAIRLY
                     ADNLDRPFDIIEGMKMELGSTAKLRTLIHYLELVSRLHDEWAAFDRNQLLAQAAFARD
                     PITEWTARTMADHPGIEREDLLDQSLERTYSASPYELFYTGGGFQTFSNFDPEDNGRE
                     LTLRQAAAHSTNLVFVRLMRDLVRFHAARLPYDARAVLEAPLSSDRSTLLHEVADREG
                     TPEQMAWLFRTKNRPAQDLRLRAQIERDAFARMTPYWRQLGFPFQRLVPSYATAIGSS
                     GDRPSALADLMGILVNDGVRSQARMVERLRFGAGTPYESVFEPTEGGSGGETVLEPAV
                     ARVTRRVLTDVVEVGTAARLKGAFTDSTGAPVWLGGKTGTGDNRFQTFGRGGRLVGSR
                     SVSRTATFTFCLGDRYYGVITSAVLGPEAARYRFTSALPLAVMRILAPSFEKELKPRG
                     APDGVQLMTALPKAATPLAPPQTLTTTTPRLETIPNGTPPTPARVQPKKTPHLIHPRE
                     NTDQPDGPERRSSPGRWI"
     gene            10156..10815
                     /locus_tag="VFR25_05680"
     CDS             10156..10815
                     /locus_tag="VFR25_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9940570.1"
                     /translation="MGITERDRIRTDSKSVEPARDREMEVREAREGTVPTERSMDVER
                     RGAAAPYSGDRDPWLASHFDFMRHFMREMSRMFEGFPGGGGGRSYSTTSWPPIEMLER
                     DGHVVVRAEMPGMRREDVRVRVEDQSLVIEGERRDDREARHEGYFESEWSYGRFARRV
                     RLPDQVDPDRVKARYDNGILEVTLDLPPRARREVPIIGADGGLRGAVEARNRESTGSR
                     P"
     gene            complement(10837..11919)
                     /locus_tag="VFR25_05685"
     CDS             complement(10837..11919)
                     /locus_tag="VFR25_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940571.1"
                     /translation="MRLRILMTADTSVDVWTFAMELATTVAQNGSQVDLALLGDPATP
                     AQRTEADAVPRLHLHEGGYPLEVETDQAGEWLMDLERRLSPDIVHLNGYVHGALPWRA
                     PVVMTGHACALSWWEAVHGPPLPATLDPYADEVRRGLRAAHVVAVPTRAMRDSVLRLY
                     GERVSVRVIPNGRTIAEPPVIPKRHRVLFVGDLRDPSKNAAAMDRVAARLSWPVVVAG
                     ETRSRNGEPIRFKNARALGRMDRASLARAYAGAAIFALPALYEPFGYGALEAALSGCA
                     LVLGDIPTLREIWGEDACYVPPRDDQALLQTLRWLIEDRHVRRDYARRAKARAVEFTP
                     ERTAASYRDAYRMALAEPSGMLGAQA"
     gene            complement(11944..12096)
                     /locus_tag="VFR25_05690"
     CDS             complement(11944..12096)
                     /locus_tag="VFR25_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940572.1"
                     /translation="MGRSSEFMQGLLVGVSLIVAGYAGITAIVWGADAVRSRRLRTGP
                     NYAFLR"
BASE COUNT         2007 a         4176 c         4380 g         1867 t
ORIGIN      
        1 cgtccaggag ctcacggctc ggccgtcgcg ccgccgactg cagggccgcg cgccggatct
       61 cgaccgaggg ctctttcagg agctcgagga gccgcggcac gcgcgcggag gacgtgacgg
      121 tcccgagcgc gcgggccacg agcacgcggt cgtcggcatt ctcggacttc atgagccggt
      181 cgacccaggc ggtcgtaacc gcgcccggcg cctcgctggg gctctcgagc agggcgtcga
      241 gcatgaagcc tcggagccgg gcgtcctcgc cctcgagcaa cgagcgcgcg tactcgacct
      301 ggcgggagct catcgccagc tcgtagctga ccgcagcccg gcacacacgg tcgtccgcat
      361 cctcgagcat ccctcgcacg cgattccggt cgtcccgcag gcgcagcgac tccatggcgc
      421 ggatcgtcga ggcgcgcacg gcgggggagg gccggtccag attgtcgagg accgccttcg
      481 cgattctcgg gtggtggatg tagcgcgcca gatcgagcgc gaggagcgtg cgcggctcgt
      541 cgggcgagcg gagcgcggcc tcgagcgccc gtctcgctcc ggggacccgc atcgattccg
      601 cggcgccctc cgcgtcgagc cagcggctgc tcagtgcctg ttcgaacgcg cgcgcgtact
      661 ggcggttgag gcggtagagg acgaagagcc atcccgcggc gatcacgacc gtggcgatcg
      721 cgatcgcact cagggccgct cccgccacgc gcatgacgat gatcaggaat accccgacgg
      781 ccgcgtccga ccatcgctcg accagcgtgt cgatcgccgg cttgatccgg cgccgctcgt
      841 ccggtcggac ggccatgtag tagatctccg ccgaggattt cccgattccc tggagcacga
      901 ccccctggac ccaccgcaac gcgacgaccg cccaccaccc cgtgctcagg gccacccacc
      961 acgagaagat catgacgagg gccggctgga gtccggtcga ggcgacgagt ccgatgcgcg
     1021 tgaggagcca gcccacggcg ccgaactgga acacgagggc gagcagattc aggacgagat
     1081 agaaccggcc gaggaagcgc gccaaggtct cggtgtccgg atagatgtcc tggatgaaga
     1141 acttgaactg gaagtcgacc agcgtcgaca cgatcacgga aagcgcgagc gccgccgcca
     1201 ggtactggag atagttggag cgccggatga tcttccagcg ggagcccgca agagcggccg
     1261 cgggggcgct tgtcgcgcgc tcctcgcggt gacgctccac gacccgcgtg agcaggaagg
     1321 acaccacgag gagggtggcg agcgcccatt cgagcgcggc acgtcccagg atcggcgcca
     1381 cccagcccgc gaacgcgccg cccaggagcc ctccgacgag tccgaatccg ctcaggagcg
     1441 ggaagacccg ccgcgcgcgc cgggaatccc acacgtccag cgcgaggacc cagaagtgag
     1501 gcaggagcat catggcctgg cttccggtcc agacgtagaa cgcggccgcg gtccagtgcg
     1561 gcgcggccgc gaatgcgggg atggcgagca ggctgatggc gatcgccgag tactggttga
     1621 tccggttcgc ggcgagacgg gagaaccgcg tcgcgaacag gccttcgacc cagacgaacg
     1681 cggccgaggc gatcgcgacc gccacgtagg cgatcggaag cgcgccggcg ccgaactccg
     1741 cgatgaaggt cgcatccctc tggaccttcg cgatcgtgta ggcggtgagc agcgccgtga
     1801 tgagcgcgca catgagcagg agaactcccc gctcctgtgg ctccagccct agccgccggg
     1861 tgagtgcgtc gaggcggtga actatggcga gctcctgcgt cggctccgcg agaagcgagg
     1921 cgactcttga ggggtggaag tgccgggaaa tccctgccat ggggatacat agccagatcg
     1981 gtgccacggg gcggcgaggc aagggaggcg cgccgggacg attggtgccg aatggtttac
     2041 gcgccgcatc tgcggcggcg ccagggtagc cccaccccat cagcggaaaa ttctacaaaa
     2101 acgccccctt tgaccgaggt tcggtccccg acgacaggat cgcaatgccg caagggacgc
     2161 tccccaaagg gctgagtgcc gttgtcactg ttcttgcaga tagccccgat catgggacga
     2221 aaacgtagcg tgagcggctt caaccggttc gccgagcgcc tctcgggagc tgtgaccgaa
     2281 tgggcgggag gcaccgcggc gttctcgatg gcgctcctcc tcatcgtggg gtgggtggtc
     2341 ctcggtccag tcttccactt ctccgacacg tggcaactcg tcatcaacac gaccacgacg
     2401 atcgtcacgt tcctaatggt cttcctcatc cagcgcgccc agaacaagga cgcgcgggcg
     2461 acgtcgctca agctgaatga gctgatcgcg tcgatcgagg gagcgagcaa ccggctggtc
     2521 gacgtagagg atctctccga ggaggagctg cggacgcttc accagcactt ccgccgtctc
     2581 gtcgagatgt cgaagcgtga cgcatcgatc atgcgctcgc attcggtcga agaagcagcg
     2641 gtccggcacg agaccaagca tgcggggcac cgcccccggg ggcggagacg gcgctcgagc
     2701 gcgcaacagg agcgcggcgg ggccgcgaag accaacaaca actccaaggc tcagcactcg
     2761 tgaccatcgt cgagcgtctc caagctcgcg gaatccgtcg cctcggctcg aaagaacggg
     2821 gcttccgcta ccgcacggca tccggagtgg ccgtcacggg cgaggaccgg gagcggatcg
     2881 acgccctcaa ggtccccccg gcctggcggg acgtggcgat ccatccgtca ccgcgggcct
     2941 gggtgcaggc cgtggggcgg gacgctgccg gccgctggca atatcgctac cacgcaaagc
     3001 aggtgctccg tcgcgagcgc gggaagcgca agcggctcct gcgcttcatc gccgcgcttc
     3061 cacggatgcg gcgcgccgtc gcccgcgacc tcgagcttcg tggtttgaag cgcgagaagg
     3121 tgctcgccgg aattcttcga atcctgtcga cgtgcttcct ccggccggga agcgaggagt
     3181 acgcgagcca gaacgggagc tacggcatcg ccacgctgaa gccgcgccac gtctccgtgc
     3241 gcggcgatcg catccgcttc gatttcgagg ggaagtcggg acggcgccag gtccgggagc
     3301 tccgcgaccg gcgcctcgcg tcactcgtgc gcgcgctcct ccgccatccg ggcgaggtgt
     3361 tcaagttcga gagtgactcg ggcgccatgg tcgacgtgcg ccgggagcac atcaacgcct
     3421 acatcatgga cgtgatggga gagaggttca gctcgaagga cttccggacg tgggcgggga
     3481 cactcctctg cgcctgcgcg ctcgcgcggg acggcgggag gaagccgtcc tcgcgaaccg
     3541 aagccaagcg ccgcgtcgcc gccgccgttc gcgaggtcgc ggagcatctc ggcaacacgc
     3601 ccgcggtctg tcgcacgtcg tatgtgttcc ccgaggtcat tcgccgcttc gagagcgggc
     3661 gcgtgatcgc cgacaagatc gaaggcgtgg acgcgctggt gaacggcagt ccccgcgcgc
     3721 tccagcgatg cgagcgcgcg ctgctcgacc ttctcacggc gacgagcggc accccacgcg
     3781 ggtccgcccg tcgcgcgcgt cccaaacccg ccctccgcct cgaggaagcc cgtgagcctt
     3841 tcggaccgac tgatcatcct gaaccccgga gcgtcgcgat accccgctga cgagatccgc
     3901 gacgtgtgcg agacggtctg ccgtgagcac ggactcgagg gtgtcttcct cgagtcgccg
     3961 acgggaccgg gcgcgcgcgc gtcgatccgg cgcgtggttc gcgaggcggt cgcgggcggg
     4021 tgcgagcgga tcgtcgccgc gggaggggac ggcacgatcg gcatggtggc cgaggcggtg
     4081 gcgtgcgccg gtgcggcgca cgacgtcacg ctcgggatca ttcccgccgg gaccgccaac
     4141 atcctcgcgc gcgagctggg cctccagggg agcctcgcgg acgtggcgga gaccgcgatc
     4201 gtgagcgacc aatcgctcgc gatcgacgcc atcgacgtcg acgggcgcct cgtcttcaca
     4261 caggtgggcg tgggacccga cgccctcatg atccgggaca cgtcgcgcga ccgtcaggaa
     4321 aagctgggcc ggctcgccta catgatcacc ttcgccgcgc gcgcgctccg ccacaggccg
     4381 cgacgcttca cgctgtccct cgacggaagt ccgatccgcg cactcgcgtg gcaggtggtc
     4441 gccgcgaacg tggggacggt gggcgcgcct ccgttcacgt gggggccccg catcgatccg
     4501 accgacggcg tgctcgatct ctgcgtgttc gacgtgcgga cgctcgccgg cacccttcgc
     4561 ctcacctggc ggatcctgac cggccgccac aagcgcggcg cctcgacgat gttctaccgc
     4621 gtgcgcgagg aagccgtgat cgactcgccg cgaccccagc tcgcgcaggg agacggcgag
     4681 atcctggggc gcactccgat cacgctgcgc gtggtccggg acgcggttcg cgtggccgtc
     4741 gcccgcgcgg tcaacgaggc ggatctcgcg ccgccaccgg caccgggcgc ggccccctcc
     4801 gccctcccgc tcgccgcggc ccgccccgag ccgagcctga ccgaggacgt ggagaccatg
     4861 atcgccgcgc accacgcgcg cacgtggatc ctccaggggg tgctgcgtca tccgatcgcg
     4921 gcgctcgaag cgctcgatgc ggcgctcttc ctgcgcctga accggctgac cctcggcccc
     4981 atccccgatc aggtcctcac gttcgcctcg cggtacatgc actacggcga gggatggatc
     5041 ctggtcctgt tcgcgatgat ggcgcaggat ctccggcacg ggctcagggt cgcggtccta
     5101 gtgctgccgg tgctctggct cacgctcctg acggtgaatc ttcccttgaa gcgcttcttc
     5161 cgccggcgtc ggccgttcct ggcgttcgtg aaggcgcggg tcctcggtcc gaggccgcag
     5221 gacttctctc ttccgtccgg gcacgccgcg gccggcttcg ccggcgcgtt cctgctctcg
     5281 ctccacgcgc cggccttcgc cccgttgttc tacgcggtcg cgatcgtcgt cggtctctcc
     5341 cgggtgtacc tgggagtcca ctacccgagc gatgtcgtga tcggggcggc ggcgggaacg
     5401 atcctcgccg cgctctaccg gtggattctg ctcgcggtcc tcccgttccg cgtctgacga
     5461 agcgccgctc gccgtggccc ctatcttgca cgacgggtgg atcacggaaa cgccgttcga
     5521 tgcaggacgc ggaaggccac gtatccgcaa cgtgtaggag ggcaggcgat gcccgtcatc
     5581 gttcaggttt gcgttgtcat tgtaacaatt gcggtcgtcg cgctcgtgat cgccatggtc
     5641 cgtatggcag accgtttgtc caaactgacc gaggacatcc gtacgtgtct cgtggaggtg
     5701 cgagaggtca ccggcgaggt caagagcgtg gcggccaccg cgcgcgaagt cctcaagccc
     5761 gtccagcgcg tcgtcgaccg cttcgagcgg ctcgggacac gcacggccgc ggtttcgtca
     5821 gcgtttctgg aggaagtgga gggcccggtc cgaacggctg tcgcgctcgt gcgcggcgtg
     5881 aagaccgggg cgggcttttt catggagcgg cttggaagcc gcttccgacc cggtcgcgtc
     5941 gcgaccgatg gagggtatcg gtatgaatga gtatccgtac ggcagcgacc tggggcaggg
     6001 ctcgtcgaac aacatgatgg gcttcttcct cggagccctg gtgggagcga gcgtcgctct
     6061 gctcttggcg cccgcgcccg gcggcgacac gcgtcggcgc gtcgcggaca cggccaagaa
     6121 gcttggctcc gcggccaagg acaaggtcgc cgagatgcgc ggccaggcta aggagatggg
     6181 cgagatgggg cgtgagtcct acatgcaggg gcggcagggc tagctagggc ggcagactcc
     6241 aacaggtcgc tcgagcgtca cacgtgaggg cccgagggtc cgtccggcag gacgggcgcg
     6301 agggccctcc gtcatgacgc gaagaatcag agggccatgc tccggccggt gccgctcgcg
     6361 gacgatccgg gctgcgacac cgtgcgcgtc aactcgcccg tcgtcttttc cgataggttc
     6421 tgcgcgatgt cggcgaggga cacgatgccg cagcaccgac cacgctcgtc ctcgacgggc
     6481 gcgcgccgca cctgacgctc ctccatcagg cggcagcact cctcgacctt ggtgctgggc
     6541 cggacggtga gcaccggccg ggtcatcacg tgctccgccg tcatggtgag cgggttgtcg
     6601 ccgcgcgcca gcgcacggca aacgatgtcg cggtcggtga tgacgccgac cgtcctcctg
     6661 ttgttccggt catcgacgac ggggatctgc ccgcagtcgt tctcgaccat caagcgggcg
     6721 acttcctgaa gcggtgtgtc cggcgtgcag cacgccgggt cgctggtcat gatgcgttcg
     6781 acttccatgg catccctcct ggatcgggtt cttcctggac tcccatctcc aatcctggaa
     6841 ggcagcaggt tccgggccag aggaggccgg tccgggtcag atccagcggc ccggagaaga
     6901 ccgacgctcg ggcccgtccg gctgatccgt attctcccgg ggatggatca aatgcggggt
     6961 cttcttcggc tgcacgcgtg ccggagtcgg cggggtgcca ttggggatcg tttccagccg
     7021 aggggtcgtc gtggtcagcg tctgcggggg cgcgagaggt gtcgcggcct tcgggagcgc
     7081 ggtcatgagc tggacgccgt ccggcgcgcc gcgcggcttg agctccttct cgaacgacgg
     7141 tgcgaggatc cgcatcaccg cgagcgggag agcgctcgtg aagcggtacc gcgcggcctc
     7201 gggcccgagc acggccgacg tgatcacgcc gtagtaccga tcgccgaggc agaaggtgaa
     7261 cgtcgccgtg cggctcacgg agcgcgagcc gacgaggcgg ccaccccggc caaaggtctg
     7321 gaagcgattg tcgccggtcc ccgtcttgcc gccgagccag accggcgcgc cggtcgagtc
     7381 ggtgaaggcg cccttgagcc gcgccgcggt gccgacctcg acgacgtcgg tgagcacgcg
     7441 ccgcgtgacg cgcgccaccg ccggctcgag caccgtctca ccgcccgagc cgccctcggt
     7501 gggctcgaac acggactcgt agggcgtccc cgccccgaaa cggaggcgct cgaccatgcg
     7561 ggcttggctc cgaacgccat cgttcacgag gatgcccatg agatcggcca gagccgacgg
     7621 ccggtccccg gagcttccga tcgccgtggc gtaggacggc acgaggcgct ggaaggggaa
     7681 tccgagctgc cgccaatagg gcgtcatgcg cgcgaacgcg tcgcgctcga tctgcgcgcg
     7741 gagcctgaga tcctgggccg ggcggttctt ggtgcggaaa agccacgcca tctgttccgg
     7801 cgtaccctcc cgatcggcca cttcgtggag cagcgtggag cggtcggagg acaggggggc
     7861 ctcgaggacg gcgcgtgcgt cgtatgggag ccgggccgcg tggaagcgga ccagatcgcg
     7921 catgagccgc acgaagacga ggttcgtcga gtgcgccgcg gcctggcgga gcgtcagctc
     7981 gcggccgttg tcttccggat cgaagttgct gaaggtctgg aacccacccc ccgtgtagaa
     8041 gagctcgtag ggactcgccg aatacgtgcg ctcgaggctc tgatcgagga gatcctcgcg
     8101 ttcgatgccc ggatggtcgg ccatcgtgcg cgcggtccac tcggtgatgg ggtcccgcgc
     8161 gaaagcggcc tgggccagga gctgattccg gtcgaacgcc gcccactcgt catggaggcg
     8221 cgagacgagc tcgaggtagt ggatcagcgt ccggagcttc gcggtgctgc cgagctccat
     8281 cttcatccct tcgatgatgt cgaacgggcg gtccaggttg tcggcgtaca ggcggatcgc
     8341 gttgccgcct ggggtgcgct cgcagagaag cacgctgtag agaacgcgct tggggtcgcc
     8401 ttgcgacagg aggtgctcgc cgcgcaggcc gcgcgcccgc acgaaggacg tgtcggccag
     8461 ctggcgcaag gtctgggtca ccttgacctg gagcggctcg tccacggtgg tctcgaccgt
     8521 caggtcgagg cggtccagct cgtacgtatc tttaagtccg agcatgctga cgagctcgtt
     8581 ccggatgcgg cgcgccccgc gcccgagcgg attctcaggc agaggagcgg tcctcgggac
     8641 cgaggcgaac ttgagcggta gcttccgcac ggtgctcgcc accttgggct cgaggacgcc
     8701 ttccttggcc aagagcccgg cgtacgcgtt cacgcggtcg ttgagcgcat tgtgatccgc
     8761 gatcaagtac cggctcggcg cccggaccga gcagaggagc gccatgaccg gcttgagcgc
     8821 cttggcccgc ttcgtcgccg acttgctgcc gaggtccttg tagaccttct tcggatcgag
     8881 gccgaaccag acggacagcc cctgatcgag ccccgcgatc tcgccgatgc ccggggctcc
     8941 ggcgagcggc atcgtgttca cgtagtcgag gatgatccgg cggcggtcgg cggtcgtgac
     9001 gggaccgtct ttgtacacgc ggaggctcgc gctcatcatc tggcggagct tgtccacgcc
     9061 ggagcccgtc cggcccccct ccgcgtgacg gtacttctcg agctggacgg cgagtgtgct
     9121 ccccccttcg accggggtcg ggatcccgat ccggctcgcg gagtagagga gcatcgcctt
     9181 gccgaagcgg gcccagtcca cagccgggtt gctgtagggg cttccctccc gatccagctc
     9241 ccggttttcg atgtagagga gcgaccgcac gacgtcgggc ggcacgtcct cgaagcgcgc
     9301 cagaccgtgc cggcccgccg tggcgtcgta aatgggcttt ccgtcctgac ccaggatgac
     9361 cagtccagcg accggcgcct cggagtaggg gggagcgatg ccgaagcggc tcaccatcgc
     9421 gagggcggtc gactgccgag cctgggccgc gatgcggaag ccgcgggact cgaggcgctt
     9481 ggagaagtct gggaggcgcg agtagccccg gcgctcgtcg agcgggccgc cgcgggggaa
     9541 cacgatccga tcgctcggcc ccggctcgac cctgtacttc atgtccgacg cgaccttggc
     9601 gaggatgttc gcctcgagcg ccgcggtccg gagctcgaag ccggccgcca gaaccacgac
     9661 cgcgatggca accatcgccg gccagcgccg ccgggaggat cgctgaactc gataccaccc
     9721 gtcacgggcg acccgggcgg gcacgggaat gccgcgcctc agggcggaca tcgcctgaga
     9781 gccggcacca agagcaacta ggagaaatgg gggaatcggt ggccgttcca aatgcgttcc
     9841 ttctttgttt gggacgctaa aaacccgaac tcgatgcgcc ggttcccgca cccgggtggg
     9901 tgagatgaac cgattcgcac aggcgttttg tacaactgtc ctatcgttcc gttccccgtg
     9961 gagcgcggac gaaaccgcgc catgtgtgtc caaccggcac ggatagctcg tcttcgctcc
    10021 aacccacgct ccggactcga cgctttcgga acgcgggcga cctcgtacga tcgcatgatc
    10081 tatgcatcca ccgtgctcga cgaccgcggg cgggagcgcc cgcatgaaaa accgagctca
    10141 gggaggagag gaatcatggg aatcacggaa cgcgatcgca tcaggaccga ttcgaagagc
    10201 gtggagcccg cgcgggatcg tgagatggag gttcgggagg caagggaagg aaccgtcccg
    10261 accgaacgca gcatggacgt ggagcgtcgc ggcgcggcgg ccccatattc cggcgaccgg
    10321 gacccctggc tggcgtccca cttcgacttc atgcgccact tcatgcggga gatgagccgg
    10381 atgttcgaag gcttccccgg aggaggcggc gggcggagct actccacgac cagctggccc
    10441 ccgatcgaaa tgctcgagcg ggacggccat gtcgtcgtcc gtgcggagat gccgggcatg
    10501 aggcgcgagg acgtgagggt ccgcgtcgag gatcagagcc tcgtgatcga gggagagcgc
    10561 cgggacgacc gcgaggcgcg ccacgagggc tacttcgaga gcgagtggag ctacggccgg
    10621 ttcgcccggc gggtgaggct gccggatcag gtcgaccccg accgcgtgaa ggctcgctac
    10681 gacaacggaa tcctcgaggt cacactggac ctcccgccca gggcccgacg cgaggttccc
    10741 atcatcgggg ccgacggtgg gctgcggggc gcggtcgaag cgcggaaccg agaatccacc
    10801 ggatccaggc cctgaccgcg cggggcgcaa ggccggtcag gcttgcgccc cgagcatccc
    10861 gctcggctcg gccaacgcca tccgataggc gtcgcggtag ctggccgcgg tccgctccgg
    10921 cgtgaactcg acagctcgcg ccttggcccg ccgggcgtag tcccggcgca cgtgccggtc
    10981 ctcgatcagc cagcgaagcg tctggagaag ggcctgatcg tcgcgaggcg gaacgtagca
    11041 ggcgtcctct ccccagatct cacgcagggt cgggatgtcc cccaagacga gggcgcatcc
    11101 cgacaaggcc gcctcgagcg ccccgtagcc gaacggctcg tagagggcag gcagcgcgaa
    11161 gatagcggcg ccggcatagg cccgcgccag cgaggcccgg tccatacggc cgagggcgcg
    11221 tgcgttcttg aaccggatcg gctctccgtt ccgggaccgc gtctcgcccg cgacgaccac
    11281 cggccacgac agccgcgcgg cgacccggtc catggccgcg gcgttctttg agggatcgcg
    11341 tagatcgccc acgaaaagga ctcggtgacg cttcggtatg accgggggtt ccgcgatcgt
    11401 gcgaccgttg gggatcacac ggacggagac ccgctcccca tagagccgca ggacggagtc
    11461 ccgcatggcg cgcgtgggaa cagccacgac gtgggcggcg cgcaaacccc gcctgacctc
    11521 atcggcgtag gggtcaagag tggcggggag aggcgggccg tgtacggcct cccaccagga
    11581 gagggcgcag gcatggccgg tcatcacgac cggggcccgc cacggaaggg cgccgtgcac
    11641 gtaaccattc agatgaacga tgtcggggga aaggcgccgc tccaggtcca tcagccattc
    11701 acccgcctga tccgtctcga cctcgagagg atacccgccc tcatggaggt gcagacgggg
    11761 gacggcgtcc gcttcggtgc gctgagcggg cgtggccgga tcgcccagga gcgcgaggtc
    11821 gacctgcgag ccgttctggg cgaccgtggt cgccagctcc atggcgaacg tccacacgtc
    11881 gaccgacgtg tcggccgtca tgaggatgcg gagtctcatc gcaccccgtt cagcgtccca
    11941 cgcctaacgc aggaatgcgt agttgggacc ggtccgaaga cggcgggatc gcaccgcgtc
    12001 ggcgccccag acgatcgcgg tgatcccggc atagccggcc acgatcaagc tcacacccac
    12061 caggagaccc tgcatgaact cggatgagcg gcccatggct cctccatcga ttgaaagtcg
    12121 acaacgtgcc atcactccat gcgacgcacg acagcaatgc gcgtgccgtt acggattcgg
    12181 cgcaccgggc cattggccgt gcatgactta gctcggagga agcgcctgat cccaccggcg
    12241 ccttgtaatt ttgttcctcg ggaaggtacg cggcccgaat tccttgacac gcggcggaac
    12301 ggtaggggac cgttgaagga cgacgtctcg agagccaatg aggagcgcgt gaacccaaag
    12361 accgtcgcac ccccagccga agccgcctcc gagctgggct ccgcacacaa cagccggcag
    12421 gtcgagcgcc
//
LOCUS       DASTKC010000141         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1052163, whole genome shotgun sequence.
ACCESSION   DASTKC010000141 DASTKC010000000
VERSION     DASTKC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052163"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFR25_05695"
     CDS             <1..252
                     /locus_tag="VFR25_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940573.1"
                     /translation="REGMEATGALWRRLVTGQILYAFGAALCLIHPYWSIAFIVVVQA
                     YFAFAPHRSHAVTHPKASPAKPPASTVTTITEEVDELPR"
     gene            236..1957
                     /locus_tag="VFR25_05700"
     CDS             236..1957
                     /locus_tag="VFR25_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9940574.1"
                     /translation="MSSPVEAPVPQLGSPESFPTLTDKQLERATAGSPARTLSPDEVL
                     FEPGQSPPGIFIVLEGQLGIFRIQDGVPTLLRTLEAGKFTGEVGILSGRPVVVRIAAI
                     TPARVATIPRERLMTLVQNDAELSEVFMRAFVLRRAELVNGGFGDVVLVGSDHCAGTL
                     RIRDFLTRNDYPFMEIDLDKERDVEKLLDQFHVALADVPVLLCRGRTILRNPSNDEIA
                     DCLGFNALIEVAQPRDVVVVGAGPSGLGAAVYAASEGLDVLVIETTAPGGQAGSSSMI
                     ENYLGFPSGISGHELARAAYAQAQKFEAKLMVARRATRLDCGRRPYVLELDHGEKIEA
                     RTLIIASGAEYRKLSVRDAARFEGAGVYYSATPMERKLCEGEELIIVGGGNSAGQAAV
                     YLADKARHVHVLVRGSGLADSMSRYLIRRIEDHPRITLRTRTEIIAVEGDRHLEKVSW
                     KDAETGESTTVPIRHVFVMAGAVPNTKWLEGCLALDESGFIKTGGDLSQEDLVLARWP
                     LGRSPHLLETCLPGVFAVGDVRSGNLKRVASAVGEGSIAVALTHRVLREWGQGLDAFG
                     LEKGAAS"
     gene            1954..2220
                     /locus_tag="VFR25_05705"
     CDS             1954..2220
                     /locus_tag="VFR25_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET9940575.1"
                     /translation="MSDLVTVFETGEPAFVALAKSVLDSAGIEFTAKGEGLQDLFGVG
                     RFPGGANLVTGPVAFQVRPEDADRAKTLLSELHHGGDEEEDRPA"
     gene            2463..3677
                     /locus_tag="VFR25_05710"
     CDS             2463..3677
                     /locus_tag="VFR25_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HET9940576.1"
                     /translation="MTGDKNDVRPFRASFPEDALVDLRHRIAAARWPEQEPVPDSSQG
                     VQLATIQKLASYWKTEYDWRQCEAKLNALPQFTTTIDGVDIHFIHVRSKQPDALPILI
                     THGWPGSVVELLKLIDPLTNPTARGGSAADAFDVVIPSLPGYGFSGKPSTTGWGPDHI
                     ARAWVELMKRLGYAHFVASGGDWGAMVSDVMAAQAPPELIGIHITWPFMVPPDIDRAI
                     QTGGQLPPDLSAEERVACERLGALYKSGLGYAQEMSTRPQTMYAIADSPVGLAAWMLD
                     HDERSYALIARVFDGKAEGLSRDDILDNITLYWLTNTAVSSGRIYWDNKFAFFAPKHI
                     DIPVAFSAFPDEVIQAPRSWAERAYSNLVYFKKHDKGGHFASWEQPELYSEDVRAAFR
                     PLRHYATSALAK"
     gene            3871..5139
                     /locus_tag="VFR25_05715"
     CDS             3871..5139
                     /locus_tag="VFR25_05715"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET9940577.1"
                     /translation="MFPELPSLQADEAILHAMGRAGSICDCSDELDTPSSLTNVAAGW
                     PIFGQLVAHDITADRSALQNHADTSALRNARAPLLDLECLYGDGPVGHPFLYQRRDPA
                     KFLLGAGGTDVPRNSEGIALVGDPRQDTQTLMSQLHLAILRVHNAFVDEARLAGVPDH
                     RVFESAARDTRWHYQWTVLHEFLPSLVGSALVSEILRDGPKWFRPSNGVFIPLEFADA
                     AYRYGHCQIRHAYRLNHETEPVPLFPDLLGFRPVPPERSIDWELFFDAGGAATAQRAK
                     KIDGRLVASLMRLPVQVTGELEDAAYHSLAVRDLQRGQGVGLPSGESLARHIGARPLT
                     PEEVGLRAVGWSGETPLWYYILREADVTSAGERLGPVGGRIVAEVLITLIDRDPTSVR
                     HADRGFEPRGSWVELLLKGAGEISDTHQRM"
     gene            complement(5165..5935)
                     /locus_tag="VFR25_05720"
     CDS             complement(5165..5935)
                     /locus_tag="VFR25_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940578.1"
                     /translation="MLDSSKSSSRWGRVAGLALILVAAVSLVPLRLQAKGGHDASDEE
                     SRTPANLRIERFAYLLVEPDGNTMGGAMRTGNGYDDAADARKAQKKLGGGPIWWIRLD
                     GSRYAIQDQKTIESVRAVYKKQDELQARILGRYDEGLEALSQRMEALHPKMEQLEERR
                     QKLDKERDALSDARDEGKDQAELASKQRKLAAELEDIERAYEPLADNSEQISREMEGL
                     SQGREKAYREWETQETENRSQLRRIADDAVRGGVARKL"
BASE COUNT         1040 a         1980 c         1925 g         1095 t
ORIGIN      
        1 cgggaaggca tggaggcgac cggggcactc tggaggcgcc tcgtgacagg gcagatcctg
       61 tacgccttcg gcgccgccct ctgcctgatc catccctatt ggagcatcgc gttcatcgta
      121 gtcgtgcagg cctatttcgc tttcgcgccg caccgatctc atgcggtcac gcatcccaag
      181 gcttcgccgg cgaagccgcc tgcatcgacg gtcactacca tcaccgagga ggtcgatgag
      241 ctcccccgtt gaagcacccg taccccagct cggatcgcct gagagctttc ccaccctcac
      301 cgacaagcag ctcgagcgag cgactgccgg gagcccagcg cgcaccctga gcccagacga
      361 agtcctgttc gagccgggac agagtccgcc cggcatcttc atcgtcctcg agggccagct
      421 cgggatcttc cgtatccagg atggagttcc cactctcctc cggaccctcg aggcgggcaa
      481 gttcacgggt gaagtgggga tcctctcggg acgacccgtc gtcgtgcgta ttgccgcgat
      541 cactcccgcc cgcgtcgcca cgattccccg cgagcgtctg atgaccctgg tccagaacga
      601 cgccgagctg agcgaggtgt tcatgcgcgc cttcgtcctc cgccgggcag agctcgtgaa
      661 cggcggattt ggcgacgtcg tcctcgtagg ctcggatcac tgcgccggta cgctccggat
      721 ccgtgacttc ctgacgcgaa acgactatcc cttcatggag atcgatctcg acaaggaacg
      781 ggatgtcgag aagctgctgg accagttcca cgtcgcgctc gcggacgttc ccgtgctcct
      841 ctgcagaggc aggacaatcc tacgtaatcc gtccaacgat gagatcgcag actgcctggg
      901 gttcaatgcc ctgatcgagg tcgcccagcc ccgcgacgtc gtcgtcgtgg gcgccggccc
      961 gtcgggactc ggagccgcgg tctacgcggc gtcggaaggt ctggacgtgc tcgtcatcga
     1021 gaccacggct ccgggcggcc aggccgggtc gagctccatg atcgagaact atctgggctt
     1081 cccgagcggg atctcgggac acgagctcgc aagggccgcc tacgcgcagg cgcagaagtt
     1141 cgaagccaag ctgatggtgg cacggcgagc gactcgactc gattgtgggc ggcggcccta
     1201 cgtcctcgag ctggatcacg gggagaagat cgaggcgcga accctcatca tcgcgtcggg
     1261 cgccgagtac cggaagctct cggttcggga cgcagcgcga ttcgaaggcg cgggcgtcta
     1321 ttacagcgcg accccgatgg agcggaagct ctgcgagggc gaggaactga tcatcgtcgg
     1381 cgggggcaac tccgccggtc aggctgccgt gtatctcgcc gacaaggcgc ggcacgttca
     1441 cgtcctggtt cgagggtcgg ggctggcgga cagcatgtcc cggtacctca tccggcgaat
     1501 cgaggatcac ccacgaatca ccttgcggac gcggacggag atcattgcgg tcgaggggga
     1561 ccgtcacctc gaaaaggttt catggaagga tgcggagacg ggggaaagca ccacggtgcc
     1621 gatccgccac gtgttcgtca tggcgggcgc cgttcccaac acgaagtggc tggaaggctg
     1681 tctcgcgctc gacgaaagcg gattcatcaa gacaggcggg gatctgtccc aggaggacct
     1741 cgtgctggca cggtggccct tgggccgttc ccctcatctc ctcgagactt gcctgcctgg
     1801 tgtctttgcc gtcggagatg ttcgcagcgg gaatctcaag agagtggcgt ccgccgtcgg
     1861 cgagggctcg atcgccgtgg ccttgaccca tcgcgtcctc cgcgagtggg ggcaaggcct
     1921 ggacgcgttc ggtctggaga aaggagctgc ttcgtgagtg atctcgtcac ggtattcgaa
     1981 acgggcgagc cggccttcgt ggcgctcgcc aagtcagtgc tcgattcggc tgggatcgag
     2041 ttcactgcca aaggcgaagg actgcaggac ctcttcggtg tggggcggtt tccgggcgga
     2101 gccaatctcg tgactgggcc agtggcgttt caggtaaggc ccgaggatgc ggatcgagcg
     2161 aagaccttgc tgtccgagct gcaccatggc ggcgacgagg aggaagatcg tcccgcctag
     2221 gcgcatcgag ccgggggcaa gcggcgacgc gttctgcccc cggccaggac gtggccctcc
     2281 cgcaagtgaa tccctcggaa caaaatgcgc gaactccgtc gtggcttcgc ccccccgacc
     2341 cataccaaac ggcccaatac catcgtccaa tagaccttcc gaatttggcg cgggtagcgt
     2401 cattggggtc gatctcccca gccgagaccg tgatttgccg ggcccactag gagggggcaa
     2461 ccatgaccgg tgacaagaac gatgtccgtc cgtttcgcgc gagtttcccc gaggacgccc
     2521 tggtcgacct tcgccacaga attgccgcgg ctcgctggcc ggaacaggag ccggtccccg
     2581 attcatccca gggtgtgcag ctcgcgacga tccagaagct cgcaagctac tggaagaccg
     2641 agtacgactg gcgccagtgc gaggcaaagc tcaatgcact tccgcaattc accacgacca
     2701 tcgatggtgt agacattcac ttcattcacg tgcgctccaa gcagcccgac gcactgccga
     2761 tcctcatcac gcacgggtgg ccgggttcgg tcgtcgagct gttgaagctc atcgatccgc
     2821 tcacgaatcc gacggcccgg ggcgggagtg cggcggacgc ttttgatgtc gtcatacctt
     2881 cgttgccggg ctacggattt tccggcaagc cgtccacgac tggctggggc cccgaccaca
     2941 tcgcgcgcgc ctgggtggag ctgatgaagc gcctgggata tgcgcacttt gtggcgtcgg
     3001 gcggggattg gggtgcgatg gtctcggatg tgatggcggc ccaggcgcct cccgagctga
     3061 tcggaattca catcacctgg ccgttcatgg tgccgccgga catcgatcgg gcgatccaga
     3121 ccgggggcca gctgccgccc gacctctcgg ccgaggaacg ggtcgcgtgc gagcgactgg
     3181 gcgccctcta caagagcggc ctgggttacg cacaggagat gtcgacccgg ccccagacaa
     3241 tgtacgcgat tgcggattcg ccggtcggtc tggccgcctg gatgctcgac cacgacgaac
     3301 gcagctacgc gctcatcgca cgcgttttcg atggaaaggc cgagggcctg tctcgcgacg
     3361 atatcctcga caacatcacg ctctactggc tcacgaacac ggcggtttcc tcgggccgca
     3421 tctattggga taacaagttc gcgttcttcg ccccgaagca catcgacatc ccggtggcgt
     3481 tcagcgcctt cccggacgag gtcattcaag cgcctcgaag ctgggcggag cgcgcctact
     3541 ccaacctcgt ctacttcaag aagcacgaca agggtggaca tttcgcgtcc tgggagcagc
     3601 ccgagctgta ctccgaggat gttcgcgcgg cattccggcc gctccggcac tacgcgacga
     3661 gtgctctagc caagtgaggg aaaccgacct ccacgaaatg atctaaacta ccccggcgag
     3721 gcgctggccc cgagcgcctc gccgacgggg gcggggcctt tcgacagcct gtgccgcaga
     3781 ggtaacgtga gtcaccgtcc accagcccat tgcctgtcag cagcgcgcca gaatctaaac
     3841 tccgagaccg gcccggcgcg gtacgcccgg atgtttccgg agcttccctc actccaggcc
     3901 gacgaagcga ttctccatgc gatgggtcgc gcggggagca tctgcgactg tagcgatgag
     3961 ctggacactc cttcatcgct caccaatgtc gccgcgggct ggcccatctt cggccagctc
     4021 gtggcccacg acatcacggc cgatcgctcc gccttgcaga accacgcgga taccagcgcg
     4081 ctgcgcaatg ctcgggctcc gctcctggat ctcgaatgtc tctacgggga cggacccgtc
     4141 ggccatcctt tcctctatca acgcagagat cccgccaagt tcctgctcgg cgccggaggg
     4201 acggacgttc cacgcaactc cgaagggatc gcgctcgtcg gcgatccccg acaggacact
     4261 caaacgttga tgtcccagct gcacctcgcg attctcaggg tccacaacgc attcgtggac
     4321 gaggctcgat tggcgggagt cccggaccac cgggtgttcg agtccgccgc gcgcgacacg
     4381 cgatggcact accagtggac ggtgcttcac gaattcctac cgtctctcgt cggatctgcc
     4441 ctggtgagcg agatcctccg agatgggccg aagtggtttc ggccttccaa cggagtcttc
     4501 attcctctcg aattcgcgga cgcagcctat cggtacgggc attgccagat ccggcacgcg
     4561 tatcgcctga accacgagac ggagccggtc ccactcttcc cggatctgtt ggggttccgg
     4621 ccggtgccgc cggagcggtc catcgactgg gagttgttct tcgacgcagg cggtgcggcg
     4681 acggcacagc gggccaagaa gatcgacggg agattggtcg cctccttgat gcgactgccg
     4741 gtccaagtca ccggtgaact cgaggacgcg gcgtatcact cgctcgcggt gcgggaccta
     4801 cagcgtggtc agggtgtcgg cctgccctcg ggcgagtccc tggcccgaca catcggcgca
     4861 cggccgctga cgccggagga ggtcggactg cgagcggtgg gatggagcgg cgaaacaccg
     4921 ctctggtact acattctccg cgaggcggac gtgacgtccg cgggtgagcg cctcggtccg
     4981 gtcgggggaa ggatcgtcgc cgaggtcctg atcacgctca tcgatcgaga tcccacatcg
     5041 gtccgccatg cggatcgcgg cttcgagccg cgcggctcgt gggtggagct gctcctcaag
     5101 ggagcggggg agatctcgga cacacatcag cgtatgtaac acctcgctcg tggtcgacga
     5161 cactctagag ctttcgcgcc accccaccgc gcacggcgtc gtccgcgatc cgccggagct
     5221 gtgaacggtt ctccgtctcc tgcgtctccc actcccgata cgccttctcg cgcccttggc
     5281 tcagtccttc catctcgcgt gagatctgtt ccgaattgtc ggccagtggc tcgtaagcgc
     5341 gttcgatgtc ctcgagctcg gccgcgagct tgcgctgctt cgacgcgagc tcggcctgat
     5401 cctttccttc gtcccgcgcg tccgacagtg catcccgctc cttatcgagc ttctgcctcc
     5461 gctcctcgag ctgttccatc ttcggatgaa gcgcctccat gcgctgactg agtgcctcga
     5521 gaccctcgtc atatcgcccg aggatgcgag cctggagctc gtcctgcttc ttgtacacgg
     5581 cccgaacgga ctcgatcgtc ttctgatcct ggatggcgta gcgcgatccg tcgagacgga
     5641 tccaccagat tgggccgccg ccaagcttct tctgcgcctt ccgcgcatcg gcagcatcgt
     5701 cgtagccgtt acctgtcctc atggcgccgc ccatcgtgtt cccgtccggc tcgacgagga
     5761 gataggcaaa ccgctcgatc cggaggttcg ccggagttct cgactcttcg tcgctcgcgt
     5821 catgtccgcc cttggcctga agcctgagcg gcaccaagct caccgccgcc accaggatca
     5881 aggcgagacc ggcgacacga ccccaacgtg acgaggactt cgaagaatcg agcatgagca
     5941 gcctcctcag gagtgcgcct ttcgtgggag atccacatga ggcgatggtg gagagggatc
     6001 gaggggcaac gccgaagtgg agcaggagct ggccgtaggc
//
LOCUS       DASTKC010000142         5194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_581860, whole genome shotgun sequence.
ACCESSION   DASTKC010000142 DASTKC010000000
VERSION     DASTKC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581860"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1462
                     /gene="guaB"
                     /locus_tag="VFR25_05725"
     CDS             20..1462
                     /gene="guaB"
                     /locus_tag="VFR25_05725"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET9940579.1"
                     /translation="MALTFDDVLLLPGYSEIHPRDVDTTTRLTREIALNIPVVSAAMD
                     TVTESALAIAIAQEGGIGIIHKNLPVLEQVEEVDRVKRSESGMIVNPITLPPDVPIAR
                     ALELMEKFRISGVPITEGKRLVGILTNRDLRFSRGSDLKVKDVMTKDNLVTAPVGTTL
                     EEAERTLHQHRIEKLPVVDQDFNLRGLITVKDIQKRIRYPRACKDHLGRLRVGAAIGV
                     GLDNVERADELMRAGVDVVVLDSAHGHSKAVLDTTRAVKQRHPTLQLIVGNIATAEAT
                     RDLIKAGADCVKVGMGPGSACTTRVVAGVGVPQITAVMECTEEAAKAGVPIIADGGIK
                     YSGDVVKALAAGASSVMIGNLLAGTEESPGETILLEGRTYKVYRGMGSLSAMSGGRGD
                     RYFQEGVKDLKKLVAEGIEARVPYKGQVANVIYQLMGGLRAGMGYCGVASIEELRTKT
                     RFVRITHAGLRESHPHDLTITKEAPNYEIR"
     gene            complement(1658..2437)
                     /locus_tag="VFR25_05730"
     CDS             complement(1658..2437)
                     /locus_tag="VFR25_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940580.1"
                     /translation="MTAARKYLASKANPRKVRPTAYVAINCLLLTKASGTPKNGDPLP
                     QEDVDAYLAALKDQIEAHVPLPPERSRLLSRFDVAAWRRLASAHRGSLRYRIFRTPED
                     LLLVSLGRFEHSGPYVPNRREGVITGEEADVGRSPNWYHFSYTYGELLENPTAAMGGL
                     LEKLTVAVERYHKMLSHMRGEYWSLGARFKKAQVIRLERSVDEDARENDLRQMQDQLL
                     DAYSEWKRTGERQHLDQMRGLTREIRLLLPDFQFSLPPGAP"
     gene            2901..3368
                     /locus_tag="VFR25_05735"
     CDS             2901..3368
                     /locus_tag="VFR25_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9940581.1"
                     /translation="MDTAATDPVKTRGLLAFRIGDVDFGARVEDVAGLVEADRLAPLP
                     GSKEPIAGLVAFRGGIVIALDLVAFLGERPASGPHRFALVLARGGDRLALVIPSLPRL
                     VAGSALSGPERHEGADADANALVDSVYDHEGSPIHCLHYWAALDGASGRGAAA"
     gene            3383..4507
                     /locus_tag="VFR25_05740"
     CDS             3383..4507
                     /locus_tag="VFR25_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9940582.1"
                     /translation="MTRRYPVIPVLSASVIVSIAAASVFLFLPMMLAYATATAVAVLG
                     AIAVAAVASRAARSREHELLHHVHRLETDKARSAELVAEAEKTRRHIEMATAMLTENR
                     DPLVESPELRVGELHDLLEAARTTRESLPVVNRIAVDEAELVGAGPDRLPGTWPRPED
                     VASDPRARAELLRGLDELIHGLEGCLAPSRAEKESAPRDGDPSGRSPTQLVDAVVQTA
                     ADGIEDLAAGLMRASELASVAERVTNRATLLALNAALEATRSGGEAFGAIAEETRLLA
                     EFAREATDTITRLAGEIEIKVGETITAIHATSEDAKAALANAGGSPAPRTSAVAAEVE
                     RLHERALELRAGLASGASRSEPEPQPAKKPRVSIRTQGKG"
     gene            4510..>5194
                     /locus_tag="VFR25_05745"
     CDS             4510..>5194
                     /locus_tag="VFR25_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940583.1"
                     /translation="MESFDTPPAPGWVSSAERLTRITATYGALIHEQVELTRRFLGQP
                     EPDPATGEGALVEEAIRLVLRGAEWLELKELVDSVQSLREAIGRIGQVTADDRKELVS
                     ACHVELETEERLAGALRSEGLGSLLDQADLVEDAIEGMRSEIQPARSQPPRSLFEEGA
                     AGLGPHGNLLALTVEIKSAVSHQNERISSIHDMAGGALHTLLATISEWEQGRKEKSLR
                     SPDAAADEER"
BASE COUNT          833 a         1774 c         1790 g          797 t
ORIGIN      
        1 gccgcgggtg gcggaggaga tggccctcac cttcgacgac gttcttctcc tcccgggcta
       61 ttccgagatc catccgcgcg acgtcgacac caccacgcgg ctcacccgag agatcgccct
      121 caacatcccg gtcgtgagcg cggcgatgga cacggtcacc gagagcgccc tcgccatcgc
      181 catcgcccag gaaggtggga tcgggatcat ccacaagaac ctccccgtgc tcgagcaggt
      241 ggaggaagtg gaccgcgtca agcgctccga aagcgggatg atcgtgaacc ccatcaccct
      301 tccgcccgac gttccgatcg cgcgggcctt ggagctgatg gagaagttcc ggatctcggg
      361 cgtgccgatc accgagggga agcgcctcgt cggcattctc acgaatcgcg atcttcgctt
      421 cagccgcggc tccgatctca aggtgaagga cgtgatgacg aaggacaatc tcgtcacggc
      481 cccggtcggg accacgctgg aagaggcgga gcgcaccctg catcaacatc gcatcgagaa
      541 gctcccggtc gtggaccagg atttcaatct ccgcggcctc atcaccgtga aggacatcca
      601 gaagcggatc cgctacccgc gcgcctgcaa ggaccacctg ggccggcttc gcgtgggagc
      661 ggcgatcggg gtcggcctcg acaacgtcga gcgcgcggac gagctgatgc gggccggcgt
      721 cgacgtggtc gtgctcgaca gcgcgcacgg ccactcgaag gcggtcctcg atacgacgcg
      781 cgcggtgaag cagcgccatc ccacgctcca gctcatcgtg ggaaacatcg cgaccgcgga
      841 agcgacgcgc gatctcatca aggccggcgc cgactgcgtg aaggtgggca tggggcccgg
      901 ctccgcctgc acgacgcgcg tggtcgcggg cgtgggcgtg ccgcagatca ccgccgtcat
      961 ggagtgcacc gaagaggcgg ccaaggccgg cgtcccgatc atcgcggacg gcggcatcaa
     1021 gtactccggc gatgtcgtga aggcgctcgc cgcaggcgcg agctcggtga tgatcggcaa
     1081 tctcctcgcc ggcaccgagg agagcccggg cgagacgatt ctcttggaag gccgcacgta
     1141 caaggtctac cgcggcatgg gctcgctcag cgcgatgagc ggcggccgcg gcgaccgcta
     1201 cttccaggaa ggcgtcaagg atctgaagaa gctcgtcgcc gaaggcatcg aggcgcgcgt
     1261 cccctacaag ggacaggtcg cgaacgtgat ctaccagctc atgggcggcc tgcgcgccgg
     1321 catgggctac tgcggcgtcg cctcgatcga agagctccgc accaagaccc gcttcgtccg
     1381 catcacgcac gccgggctcc gcgagagcca cccgcacgac ctcacgatca ccaaggaagc
     1441 cccgaactac gagattcggt agcgcccgct cctcacgccg gcgctctcgt tgccccgccc
     1501 gtcccgcgtt ctcccgcaag tttcgttcgc cctgaaaacg ccgatcgccg gctcgcttgt
     1561 ggcgaccggc gatcgcgtgt gtgcaggggt gcgtgccctc cggggtgcgt cgggtcgggg
     1621 gagggaccag agggcacgcg ggggatcgag tcgcttccta cggggctccc ggcggaagcg
     1681 agaactgaaa gtcgggcagg aggagccgga tctcgcgcgt cagaccgcgc atctggtcca
     1741 ggtgttgccg ctcgcccgtg cgtttccatt ccgaataggc gtcgagaagc tggtcctgca
     1801 tctgcctgag atcgttctct cgcgcgtcct cgtcgacgct tcgctcgagc cggatcacct
     1861 gtgccttctt gaaccgggct ccgaggctcc agtactcgcc gcgcatgtgc gagagcatct
     1921 tgtgataccg ctcgacggcg accgtcagct tctcgagcag accgcccatc gccgccgtcg
     1981 gattctcgag cagctctccg tacgtgtacg agaagtggta ccagttcgga ctgcggccca
     2041 cgtcggcctc ttcgcccgtg atcacgcctt cgcggcgatt cggcacgtac gggcccgaat
     2101 gctcgaaccg gccgagcgac acgagcagga ggtcttccgg agtccgaaag atccggtacc
     2161 ggagcgagcc gcgatgggcg ctcgccagac gccgccaggc cgccacgtcg aatctcgaga
     2221 ggagccggga acgctccggc ggcagcggaa cgtgcgcttc gatctggtcc ttgagcgcgg
     2281 ccagatacgc atccacatct tcttgaggaa gcggatcgcc gttcttgggc gtcccgctcg
     2341 ccttggtcag taggagacag ttgatcgcga cgtaggcggt cggtcgaacc ttcctgggat
     2401 tcgccttgct cgctagatac ttgcgggctg ccgtcatggc cacctcaccc cttcctatcc
     2461 attccccgac tggcttcagc tcctttggcc gaagcaactc gactacgttc gatcatcggc
     2521 ttctgcattg agcatgccga cggtttcgcg ggatcccggc ggcgacggaa tgtgcggccc
     2581 ggcgacggat tagcgggttc aggtcgtttc gccatatagg aaggcgaggt cgaaaccatt
     2641 cacgatgtgg cttcttttgg ccgattttcc gggtggaagc gcccatcgaa ggggcgtcgc
     2701 cgcgcattgc gcgatgcagg tcgaaatgca tcctgcgcgg tgaccaccgg gcgagagttg
     2761 cccggtccta cggagaggag atgggcgcgg ccaggtcaaa gcattggcgc gcaggtgctt
     2821 ggcgaatggc tggagtggtc catgatggca cggttgttgc cgtctactgc tccaatccaa
     2881 ccaccaggag cgagaccatc atggacaccg ctgccacgga tcccgtgaag acgcgcgggc
     2941 tcctcgcctt ccggatcgga gacgtggact tcggcgcgcg cgtcgaggac gtcgcggggc
     3001 tcgtcgaggc cgatcgtctg gcgcctttgc cgggaagcaa ggagccgatc gcggggttgg
     3061 tcgcgttccg cggcggcatc gtgatcgcgc tcgacctcgt cgcgtttctc ggcgagcggc
     3121 ccgcgtcggg accgcatcga ttcgcgctcg tgctcgcgcg cggaggcgac cggctcgcgc
     3181 tcgtgatccc gtccctgccg cggctcgtgg ccggatccgc actctcggga ccggagcggc
     3241 atgagggcgc ggacgccgac gcgaacgcgc tcgtcgattc cgtgtacgac cacgaaggct
     3301 cgccgatcca ctgcctccac tactgggccg cgctcgacgg cgcgagcggc cggggagccg
     3361 cggcgtgagg gaaggagagg gcgtgacccg ccgctatccg gtgatcccgg tgctctccgc
     3421 gtcggtgatc gtgtcgatcg ccgcggcgtc ggtgttcctc ttcctcccga tgatgctggc
     3481 gtacgcgacc gcgaccgcgg tcgccgttct cggcgcgatc gcggtggccg cggtggcctc
     3541 gcgcgccgcc cgttcacgcg agcacgagct cctgcatcac gtccaccgtc tcgagaccga
     3601 caaggcgcgc tccgcggagc tcgtggccga ggcggagaag acgcggcggc acatcgagat
     3661 ggcgaccgcc atgctcacgg agaaccggga cccgctcgtc gagtcgcccg agctgcgggt
     3721 gggggagctc cacgatctgc tcgaagccgc gcgcaccacg cgcgagtctc tcccggtcgt
     3781 gaatcggatc gcggtggacg aggcggagct ggtgggagcc ggtcccgacc ggttgccggg
     3841 gacgtggccg cgtcccgaag acgtcgcgtc cgacccgcgc gcgcgcgccg agctcctgcg
     3901 cggtctcgac gagctgatcc acggactcga gggctgcctg gcgccgtccc gcgcggagaa
     3961 ggaatccgct ccgcgagacg gcgatccatc cggacgctcg cccacgcagc tcgtcgacgc
     4021 cgtggtgcag accgcggccg acgggatcga ggatctcgcc gccggcctca tgcgcgcgag
     4081 cgagctcgcg tcggtggcgg agcgcgtgac gaatcgagcc acgctgctcg cgctcaacgc
     4141 cgccctcgaa gccacgcgct ccggcggcga ggcgttcggc gcgatcgccg aggagacgcg
     4201 gctgctcgcg gagttcgcgc gtgaggcgac cgacaccatc acccggctcg cgggcgagat
     4261 cgagatcaag gtgggcgaga cgatcacggc gatccacgcc acgagcgagg acgccaaggc
     4321 cgcgctcgcc aacgccggcg gatcgcctgc accgcgcacg tccgcggtcg cggccgaagt
     4381 cgagcgcctg cacgagcgcg cgctcgagct tcgcgcgggg ctcgcgtcgg gcgcctcccg
     4441 ctcggaaccc gagccacagc cagccaagaa gccgcgcgtt tcgatccgga cccaggggaa
     4501 aggctgatct tggaatcgtt cgacactcct ccggctcccg gatgggtcag ctccgccgag
     4561 cgcctcacgc ggatcaccgc aacctacggc gcgctcattc acgagcaggt ggaattgaca
     4621 cgccgcttcc tcgggcagcc ggagccggac cccgcgaccg gcgagggggc gctcgtcgag
     4681 gaggcgatcc gtctcgtgct gcgcggtgcg gagtggctgg agctcaagga gctcgtcgac
     4741 tcggtgcaat cgctccggga ggccatcggg cggatcggac aggtgacggc cgacgaccgc
     4801 aaagagctcg tgtccgcctg tcacgtggag ctggagaccg aggagcgcct ggccggagcg
     4861 ctccgctccg agggacttgg ctcgctgctc gatcaggcgg acctcgtcga ggacgcgatc
     4921 gaaggcatgc gctccgagat ccagcccgca cgctcgcagc cgcctcgctc gctgttcgag
     4981 gaaggcgcgg ccgggctggg accgcacggc aaccttctcg ccctcacggt cgagatcaag
     5041 agcgcggtgt cccaccagaa cgagcggatc tcgtccatcc acgacatggc cggcggcgcg
     5101 ctccacacgc tgctcgccac gatctccgag tgggagcagg gccggaagga gaagtcgctt
     5161 cgctcccccg acgcggccgc ggacgaggag cgct
//
LOCUS       DASTKC010000143         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_819048, whole genome shotgun sequence.
ACCESSION   DASTKC010000143 DASTKC010000000
VERSION     DASTKC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819048"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /gene="rimM"
                     /locus_tag="VFR25_05750"
     CDS             <1..482
                     /gene="rimM"
                     /locus_tag="VFR25_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9940584.1"
                     /translation="LRGVVVVESFSDDPERFAAKSELSATLPSGERRVLKVVKSKPFQ
                     ARLIVYFEGVGDRTAAEALHGADLTVPRSAVKPLSDGRHYRFELMGLTAKTPRGEEVG
                     RVADVFSTGSNDVYVLEGPRGEILLPALPEVIVSVDGEGGVLVVEPPQGLPGWDDEG"
     gene            482..1225
                     /gene="trmD"
                     /locus_tag="VFR25_05755"
     CDS             482..1225
                     /gene="trmD"
                     /locus_tag="VFR25_05755"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9940585.1"
                     /translation="MRFTILTLNPGFFEGPLGEGMIRIGREKGIVDVALIQIRDFAND
                     RYGTTDDYPYGGGAGMVMKVDTIARAFESVADAVPGAKPRVLATSPQGRVLDQSYARE
                     LAAEKHLVVLCGRYKGIDERVMPTLEAEEVSIGDYVLSGGEAAALVILDAVTRLQEGV
                     LGHAESAEADSFSEALLDAPVYTRPEDFRGLKVPDVLLSGNHEQIRIWRRREAIRRTL
                     RKRPDLIRERVWSAEDRKLLNEIEKEAGR"
     gene            1222..1584
                     /gene="rplS"
                     /locus_tag="VFR25_05760"
     CDS             1222..1584
                     /gene="rplS"
                     /locus_tag="VFR25_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9940586.1"
                     /translation="MSVLERFETRFKTDRAMDFSPGDTVKVHVRVVEGEKERSQIFHG
                     VVTSVRGSGMRSSFTVRKVSGGIGVERTFPLHSPSVAKIELDRKGKVRRAKLFYLRGR
                     KGKAAKVAEREKITGDGA"
     gene            1574..>2144
                     /locus_tag="VFR25_05765"
     CDS             1574..>2144
                     /locus_tag="VFR25_05765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET9940587.1"
                     /translation="MARKGLTRFDDEYRSRWGELLAGIDEAGRGPLAGPVVAACVAFP
                     AGVKIRGVRDSKEMTAAEREEAAVRIRAKAVAVGVGVGSVEEVDAHNIRLASLLAMRR
                     ALTALGLDPQGLLVDGKDPVWCEIPCEAVVDGDAKSLAVAAASIIAKVTRDALMDAEE
                     LLYPQYGFVRNKGYGTPEHLSALKEHGPCG"
BASE COUNT          363 a          624 c          826 g          331 t
ORIGIN      
        1 gcctgcgcgg cgtggtggtc gtcgagtcgt tcagcgacga tcccgagcga ttcgcggcca
       61 agagcgagct ctcggcaacg ctcccctcgg gggagcggcg cgtgctgaag gtcgtgaagt
      121 cgaagccctt ccaggcgcgg ctcatcgtgt acttcgaagg tgtcggcgat cgcacggcgg
      181 cggaagcgct ccacggcgcg gacctcacgg tcccccggag cgcggtcaag ccgctgagcg
      241 acggccggca ctaccggttc gagctgatgg ggctcaccgc gaagacgccg cgcggcgaag
      301 aagtgggccg cgtggcggac gtgttctcga ccggcagcaa cgacgtgtac gtgctcgagg
      361 gcccgcgcgg cgagatcttg ctgccggcgc tccccgaggt gatcgtctcg gtggacggcg
      421 agggcggcgt cctggtcgtg gagcctcccc aggggctccc cggctgggac gacgagggct
      481 gatgcggttc acgatcctga cgctgaaccc gggcttcttc gagggcccgc tcggcgaggg
      541 gatgatccgg atcggccgcg agaagggcat cgtcgatgtc gcactgatcc agatccgcga
      601 cttcgcgaac gaccgctacg ggacgacgga cgattacccg tacggcggcg gcgcggggat
      661 ggtgatgaag gtcgacacca tcgcgcgcgc gttcgagagc gtcgcggacg cggtgcccgg
      721 cgcgaagccg cgggtgctcg cgacgagccc gcagggtcgc gtgctcgacc agtcgtacgc
      781 acgggagctg gcggccgaga agcacctcgt ggtgctgtgc gggcgttaca aggggatcga
      841 cgagcgggtg atgccaacgc tcgaggccga agaggtctcg atcggggact acgtgctctc
      901 gggcggcgag gcggccgcgc tcgtgatcct cgacgcggta acgcggctcc aggaaggggt
      961 tttggggcac gcggagtcgg ccgaggcgga ctcgttttcg gaagcgctgc tggacgcgcc
     1021 cgtgtacact cggccggaag atttccgggg actcaaggtt cccgacgtcc tgctgagcgg
     1081 caatcacgaa caaatcagga tttggcggcg ccgggaagca atccggcgaa cgctacggaa
     1141 gcgcccggac ctcatccgtg agcgcgtgtg gagcgccgag gaccggaagc tcctcaacga
     1201 gatcgagaag gaggcaggac gatgagtgtg ttggagcggt tcgagacgcg attcaagacc
     1261 gatcgcgcga tggacttcag tcccggcgac acggtgaagg tgcacgtgcg cgtcgtcgag
     1321 ggcgagaagg agcgctcgca gatcttccac ggcgttgtga ccagcgttcg tggaagcggt
     1381 atgcgctcga gcttcaccgt gcgcaaggta tcgggcggaa tcggcgtcga gcggacgttc
     1441 ccgctccact cgccgtccgt cgccaagatc gaattggatc gcaagggcaa ggtgcgtcgc
     1501 gcgaagctct tctatctccg cgggcggaag gggaaagcgg cgaaggtggc ggagcgggag
     1561 aaaatcacag gagatggcgc gtaaaggact gacgcgcttc gacgacgaat atcgctcgcg
     1621 ctggggtgag ctcctcgcgg gcatcgacga ggccggacgc ggccctttgg ccggccccgt
     1681 ggtggccgcc tgcgtggcgt ttcccgccgg cgtgaagatt cgaggcgttc gcgattcgaa
     1741 ggagatgacc gcggcggagc gcgaggaagc ggcggtccgg atccgggcga aagcggtggc
     1801 ggtcggagtc ggggtgggga gcgtcgagga agtcgacgca cacaacatcc ggctcgccag
     1861 cctcctcgca atgaggcgtg cattgacggc cctggggctc gatccccagg gccttttagt
     1921 tgatgggaag gaccccgtct ggtgcgagat tccgtgcgag gcggtggtcg acggagacgc
     1981 gaagtcgttg gcggtcgccg ccgcgtccat catcgcgaag gtgacgcggg acgcgctgat
     2041 ggatgcggag gagctgctgt atccgcagta tggtttcgtg cgtaacaagg gatatgggac
     2101 gcccgagcat ctgtcggcgt tgaaggagca cgggccgtgc ggga
//
LOCUS       DASTKC010000144        21832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2003350, whole genome shotgun sequence.
ACCESSION   DASTKC010000144 DASTKC010000000
VERSION     DASTKC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21832
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003350"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFR25_05770"
     CDS             <1..190
                     /locus_tag="VFR25_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940588.1"
                     /translation="NLRSAAIRVTSGPADDSHPRFSADGNTIYFISNRTNNYGLNGVF
                     STERRGYNIWGVTRFDRP"
     gene            255..617
                     /locus_tag="VFR25_05775"
     CDS             255..617
                     /locus_tag="VFR25_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9940589.1"
                     /translation="MALYVCLIFGMSSIRGLQVPGPFPFMDKIAHLMEYSLFGLLVGR
                     AIRFTMSGSSRVTMSIAAISFGAFVGMCDEIYQRFVPGRSSDVFDWLTDVTAVTLAVV
                     ITQVVSARPLGRRAAPER"
     gene            664..1968
                     /gene="hisS"
                     /locus_tag="VFR25_05780"
     CDS             664..1968
                     /gene="hisS"
                     /locus_tag="VFR25_05780"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007069143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET9940590.1"
                     /translation="MASKFQVPRGTRDILPGESERWIALEATTREILSRYGYREVRTP
                     LFESTDLFVRSVGESTDIVRKELYTFEDRKGRSLTLRPEGTAPLVRAYLEHALGHGDP
                     VTRLYYIGPMFRYERPQAGRYRQFWQIGAELLGPAAPAADAEMIDLFVAILQGIGLRD
                     VRVLVNSLGDSVCRPVYRERIREHFASREAELCGDCKERLRTNPLRILDCKVPSCQPA
                     IASAPSVLDSLCDPCREHFEGVKKALDALSIEHETAPRLVRGLDYYTRTVFEVHAAGL
                     GAQNAVGGGGRYDQLVKDFGGPETPAIGFSIGMERLLLATGSLETGSALRPDVCVIAR
                     EPGAVLEALKAARELRGLEADGRVAGLRVLVDVAGRSTSAQMKWASKLEARYAVFVPP
                     DSGGYAVRDLEAGQDAPKHATLPQLRAWLRQQTATVSEDVIR"
     gene            1965..3764
                     /gene="aspS"
                     /locus_tag="VFR25_05785"
     CDS             1965..3764
                     /gene="aspS"
                     /locus_tag="VFR25_05785"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET9940591.1"
                     /translation="MIEPFRARKRTHTCGDLRPENAGMKARLLGWVHRTRDHGGVLFL
                     DLRDRYGITQVVFRPEHLGDEAMERARSIGSEYVVMVEGKVLNRPRGSENQDLPTGGI
                     ELEAEALAILNTSKTPPFPLDETATASEDLRLRYRYLDLRREALQSSLIFRHKMTRSV
                     REHLSDQGFLEIETPMLVRPTPEGARDYVVPSRLHPGKFYALPQSPQLYKQILMVSGF
                     DRYFQIARCLRDEDLRADRQPEFTQIDLEMSFVTEDDVFEIVEGMLASVFRRDLKRDL
                     KIPFPRLTYRESMERYGSDKPDLRIPFEIKDVTDAAGQSAFEKFKEARAGGPMLMTGA
                     LAVPALTPPASRREIELWEAAGKAQGLAGLAWTRVKGGALEGGVAKQFGPEAQKALIE
                     AAGAREDSLLLLASGPRATVQKGLGHVRLQVGQAKFALDAQDFRFVWVHQFPLFEYLE
                     ERKQWAPAHHIFTMPLDEHLAHLTTDPGKVHAQLYDLAANGQELASGSIRIHRKDIQE
                     KVMGVIGMSLEEARAKFGFLLEAFEYGAPPHGGIALGLDRLVVLFHGGDSIRDSIAFP
                     KTARATSLMDDAPGPIDEADLHELHLNVTRRKD"
     gene            4282..4845
                     /locus_tag="VFR25_05790"
     CDS             4282..4845
                     /locus_tag="VFR25_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9940592.1"
                     /translation="MTAALAILVTVVAGCGKPVLRVADASLGDYYTEKEYRNLSDEQR
                     AEYCQELSEQRDLFREQIEGAEEALDALHQTVGPLRAEVDSLTARAEELERRLAEARA
                     SGGTNLMKGGAEASRSAASGPASARESTPASDRHVVKPGESLWSISAKSDVYGEGARW
                     NRLYRGNKNRIKNPDRIYPGQEIQIPR"
     gene            4842..5852
                     /locus_tag="VFR25_05795"
     CDS             4842..5852
                     /locus_tag="VFR25_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9940593.1"
                     /translation="MNSPAAETTKSVPIPDVDPLSLFGKNDANLRLVERSYPVSLTYR
                     DGKIAVRGQAGAVDEVSTVLHALADLVRRGAVLEEHDVNNFIGLNNAADQNASLELYE
                     HPVLFSFDKRTIRVKTLGQSRYVNALQKHDVVFAIGPAGTGKTYLAVAAAVHALKSRQ
                     VERIVLVRPAVEAGENLGFLPGDMKEKVDPYLRPLYDALNDLLSYEKIRRFLDLGVIE
                     VAPLAYMRGRTLNNAFVILDEAQNTTVGQMKMFLTRLGYGSKAVITGDVTQIDLSTDH
                     ASGLVEIQTILTGVPDLSFVYLNERDVVRHHLVREIIRAFDEHEKAQPPRAQTSQGSG
                     PA"
     gene            5849..8104
                     /locus_tag="VFR25_05800"
     CDS             5849..8104
                     /locus_tag="VFR25_05800"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9940594.1"
                     /translation="MSTEPLRVLESRRREKRNGPKAKFLRLVGHRSRRTMLLLGFLLF
                     TCLLIPREPDFEVITLREGFPAKRDVIAPFQFYLLKDRALYHAEQASAARKVPPVYSV
                     DPAVAPQVHAALDSLLGDGSKHGAVARPRLEGLGLSAATNRVLLGPDGPRVVVLAQRI
                     AEEAYAVGLMAEIESGRLETDAGAASVQRDGMRVPVAWGSLFDLDSVREKAWDDGARA
                     FPNRPEAARALQELIAAVVTPNLRYEASTTERIRTESRNAVEPYDGLVRKDEKIIGSH
                     EIATALHIRKLESLRAWNDRARPKDAWRQDLLALLARLALISFLIAGFLRYLKLNHAR
                     VYGDVNLLSLLAVLTALVMGVGWLVVSQFRGYEMLIPITVLSLSAALLFGQALAFAAT
                     LVASLLIGVVFGLGLPFLTGSALAGIAAISTARGVRRRRDFYRPMVVIAAAYAFAIIA
                     MGLVDGGQGIVLLRRGLWGVVAGFASVLAVTLLLPLLESLFSVTTDITLLELADLNRP
                     ILRRLMLEAPGTYHHSLVVGSLSEAGAAAIGGNPLVARVGAYYHDIGKIEKAEYYVEN
                     QSSARNRHEKLSPTMSCLIIEAHVREGADIARKERLPRAIIDAILEHHGTTLMTFFYH
                     KAQNADPAVEERDFRYPGPKPRSKETAVLMLADAVEAAARSLSEPTPSRIRGVVTRIL
                     DARVEDGQLDESPITFEDLAKVRESFIPILTALFHARVDYPGTPASERGRRPDSRRVL
                     GEKSRLKIDHS"
     gene            8052..8486
                     /gene="ybeY"
                     /locus_tag="VFR25_05805"
     CDS             8052..8486
                     /gene="ybeY"
                     /locus_tag="VFR25_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9940595.1"
                     /translation="MRVAFSGRRAGLRSTTLKALVRSALDRLGHPRAGLDVILTDDAY
                     VRTLNRDYREVDKPTDVLSFPFAEPATLSDPDRAVFLGEIYVSLETAAAQARAARRPF
                     EREVAHLIVHGTLHLLGHDHRTRSERRRMAALEARLLRAPLV"
     gene            8494..9102
                     /locus_tag="VFR25_05810"
     CDS             8494..9102
                     /locus_tag="VFR25_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET9940596.1"
                     /translation="MFARLRTYFLTGLLVLSPVVLTGYVVWKLFVFVDHLLGTTMRGG
                     YLRPGGIPGIGFLTVIALILLTGAVANTFLARTLGSLLESLLLRVPFLRGLYTILKEI
                     GEALLGDRRQAFQRVVLIPYPSPDLYSIGFVTTPPPPSMVEALGEPVEGVFVPTPPNP
                     ATGQLVYYPKRKLIPTNLKIDQAIKIVVSCGVVVPSDPAMRA"
     gene            9105..9881
                     /gene="recO"
                     /locus_tag="VFR25_05815"
     CDS             9105..9881
                     /gene="recO"
                     /locus_tag="VFR25_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9940597.1"
                     /translation="MAIVQTDALVLRSYRLGETSLIVHLFTDAHGLLRCVAKGARGPK
                     SRFGGCLEPGVRLSAVIYRKAERDLQLLSKADITAYWPALWDDPDRFARAQAVLEFLE
                     RAAYGEAEDATLLAFAVATLAAMAEAPVPALEAVLRGFEVRACTLLGYAPELSSCLEC
                     QKPLGQGGLFHPLRGGLLCGEPCGGDAASFRISERARKTLIALGEREPASIGTWAMER
                     YPGPEISRAIERFLSAHLERFDGLRSTRVGERLARYAEGA"
     gene            9878..10801
                     /gene="glyQ"
                     /locus_tag="VFR25_05820"
     CDS             9878..10801
                     /gene="glyQ"
                     /locus_tag="VFR25_05820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET9940598.1"
                     /translation="MTAQAPRTFQETLLRLEQFWSDQGCALLQPYSSEVGAGTFNPAT
                     FFRVLGPEPWKAAYVEPSRRPKDGRYGENPNRLQLFYQYQVVLKPAPEDVLEVYLDSL
                     RHLGIDLARHDVRFVEDDWESPTLGAWGLGWEVWLDNQEITQFTYFQQAGGYDLEPIT
                     AEITYGIERIACYLQGVDRIMDLQWSRDHTWADLFLQAEIEWCGFNFEHSDPALLFEL
                     FEKFEREAYRLLKAGMVLPGYDYVVKCSHVFNLLDARGAIGVTERVASIARVRKMARA
                     AAGAYLKQREELGWPLLKGAATPEAAVATRG"
     gene            10794..12914
                     /gene="glyS"
                     /locus_tag="VFR25_05825"
     CDS             10794..12914
                     /gene="glyS"
                     /locus_tag="VFR25_05825"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET9940599.1"
                     /translation="MAETLLLEIGCEEIPAGFIAPALLELETLARDGLADARLAHGAV
                     RTLGTPRRLTLIVEGLADRQEDRVKEMTGPAVRVAFDAQGQPTQAAKGFAKSAGIPVE
                     SLERVTTPKGEYLLARVNETGAAAADVMPARLASWIASLHFPKTMHWNGPGRFARPVR
                     WIVALLGDRVLSVEAFGVVSGRRTRGHRVLAPGWLDVPSPAAYAASLEGAGVIVDPDA
                     RRVKIREELARTAAGLEGRAVEDEELVDEVANLVEWPEAVVGSFESRYLELPRPVIVA
                     SMRAHQRYFAVERPDGALLPNFVMIRSGRGPGAEEIRRGTEIVLRARLEDARFYWVND
                     LKGGLEGKLPVLKGIVVHERLGTVHDRTERLVVLVEDLAKKLAPKSLTAASRAARLCK
                     ADLASEMVRSGKEFASLQGIIGGEYAAASGEPADVAEAIREHYRPEGPSDPIPRTPEG
                     KLVALADKIEATTGGLRAGLEVTGSQDPFGLRRAGNGIVRILVESGWRLDVLDTARFM
                     DGIYDKAGVPPGDPDRFAEFWKQRVASALEVYEISSETAGAVLAVRPGDPIDVLARAR
                     AIEAIRKSPDFEGLMIGYRRAANILRSAPPADIPASGQPLAERAEAFGDRAEADLHLE
                     TKMARQAVETYLKGALPDYGVLLRHLLGLRPSIDRFFDAVMVMVDDPIARKRRLGLLE
                     AVRQTFLRIADFSLLPSAPGQKSN"
     gene            13366..17229
                     /locus_tag="VFR25_05830"
     CDS             13366..17229
                     /locus_tag="VFR25_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9940600.1"
                     /translation="MRKTLFAMLGLVAIGLWAIPVVAAPSGLVSDGPSKVRTDPVPLD
                     ARAGAVGAAARGERYAGTLSGSLIKQSAVTTNYFLYPGACVQRALGTWAAKTSPAADS
                     LQPNASFPNSSGYTAGQPNPASNTIAYTRTDLSLPEKLWHIESSSTPAGQRPAILDGT
                     RMIWCGKFDANWTDDVGYPNLTFQILYIDTGTHANPGGKYHLNFQGNFSSELNYDYVT
                     FIGGGSGGALDPIGNSRIFLSDMLPPVGSPHGGPGGDSDQLVSFTGAIRTSQTISMNS
                     GADLQIITGDASLNPVTQSYSLEVDDDHRGMYVVFTADCLFSSEDGLWPNSDGQSMDD
                     IEVDNNGVVSSIYTDAATFVPPAGVSDQDASNGTVLVWSGNVIPQAGDDTPIPIISSR
                     VPPGVGERWQLSTGASNVTQDVCSPQKALANDLFFEGGDLATHTAINKQYNAIVPCVF
                     PIPAGSAQVLVFWNEYLDIPRFSGYVQQAEFRIFKFGAWGQWNNTAPGGGVTTGANQA
                     WLVDGDDISTATQADSVQLRFELQCIPPFAADRANCATGQINPILYDDLRLQVVTGVP
                     APFFGIFPGSVAQTTFVDGRIGTAGIGQNLTNCTAGNLSAGQCWPGNRGSGLGTQQSH
                     NIAVNNNFNAPVGDTITLSLLTGLRKNGMGINWKHGFNKSINAGEFGPPGSEYAHTNP
                     TFNANCDMPRMIFRLFDPSSKTWSPWDSTELVANAVAPGPTDTTLVDSEFNIDWPPYD
                     KAIANASIPGGFTINGVGNYNALSFLPRGARMQYYFKAVDIGNGSGAGCGVSYQFSSD
                     NLAREVEDIPTLPNSSIKAPDIIEFRVLPSVYPAGPAGSLLAGRTNTPVLNLDAVYTT
                     WAFGYDPMTQALRALGVRADRYRHTASGTTAHGFGGHELPGKRPDRLSNFFPNYLEYP
                     LVDSLASWYRIVIESGHTRTSSVFNEEDAFTLEQWWRKETGTNGGDRCAFLSGDDQMY
                     QMITPSGVDATFQISMAGQVFGVASAQNSWSPATSYFNMDDRFAAATAGPALAAPNTF
                     VYPIDGGCPAPNRFDALTAAGPSGDTPFIAAVYPSGQAAAVGRMRELDNIGDNDRNKA
                     IAYGFSLQFIRDPAISNTSANYTRSGVENRMRVMYKFLTSCRTAQVGPSTCFPCPNPT
                     PQTIASMQADWSGQNAGFQTATYGSLYPIQAGALATAVGEDAPEAAPRINKIDGNYPN
                     PFNPHTAIRFSSAQAGKAQIRIFSVSGALVRTLHTTVTAGPNEVRWNGKKDDGASLAS
                     GVYFYKIVFPNGESFRAPNNLVLVK"
     gene            17941..19428
                     /locus_tag="VFR25_05835"
     CDS             17941..19428
                     /locus_tag="VFR25_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET9940601.1"
                     /translation="MDRNRPPQTFLVAAPVDTSVADIAYSYRIHLYWRGEDSDGYVVG
                     FEYSWDDSSIGAFKFTTKTDSIFELTVNDSSQISGGTGQNPSTARTHTFYIRAVDNLG
                     KPDPSLTIFNRRPFKAQTDAPIVTFTGAIPNPQFPAVLDTVCEGAPFTVSWNARDPDG
                     TVTKYRFDVGAYHSPFTTDSSVTFNDPSVPGSVDLPSGVYTMTVTAIDNANAVGTGST
                     QFIVNHDPETVLARSDARHLVGHYIQHFLGGQQVNIEGTFTFDPGDPAGGSATVPYRS
                     TVWWEWDGNDSHDGCEADCLSGFSIVLNPGGHNENNPYIVGFTDSIGTDPSGNTVHFK
                     TNNPAVLSQYGFDQLILDSLDAGRNLTIRVAARDCSGRADGTPAYFQFNCNFAPTLDS
                     VTVTPTMFDFDGNGPDPAEPARIIRWVGLDYEDGRSRGAVVKLDDTRFLTFENYEDFT
                     IVADRDFQVLSTGTTHTVKVRVSDRAGYLSDLPETEVIFQIPLTP"
     gene            19458..21737
                     /locus_tag="VFR25_05840"
     CDS             19458..21737
                     /locus_tag="VFR25_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940602.1"
                     /translation="MRRFMVLTGLAAAVLGLLYLTGCGDKVTPPPPHQDTNPETELTY
                     APLPGDTTAFRVHFYWNGYDKDGEVISFSFAVDADTLKDPKDWPTTTSKDTTLLFLVD
                     PVREVRQHVFKVAARDNDGQWDQSPAFRFFSAKTLPPTSLIKRGPAAFNPIVGPNFTF
                     EWEGIDPDGSETGGKTPVDSFEYVLLLIGATGAPDADVLPQYNQDFYLNLVNQSTGPT
                     LKSDFEGLGRIWNFSDWHWIGIHSLKNRFRNATPGEYVFAERAVDVAGATEKDLGFGT
                     NFRHFTVSTKNPGPSLIISSSVLTLPLPAAVGPGDTPRKPLQIFEGETISFSWTASAD
                     AYGGEIVGYTYALDDTSTFPGLDLLRTGATFRPAQLPPGNHFLFVRAVDDGGLITNAV
                     IPMKIVHPDFKDPGHPHSIAYVDDSTPPGVTIDRTQAIGNFPRDLDEDRFWESDPGIL
                     VGLAPFNQWDASDNGYGEVEGRKPPDPDWLKDYSTVIWNVDFNNGVGNPTGLWRTLVG
                     GAYSELAGYLRAGGTMILSGFLIGGNVINPNTVLYGNVSRGICFALEPGTTEYFLGTF
                     PRMFMGVDGAVLGGPDLRTLGGKDFIQARPTPAGIAMGFDTVDVDRGPLGSGAKWITY
                     SGFGSLNENLAPGLLYVDGLVMARNFACVDQPNSVFRLEDPTTPIAAPIYTYHGVHSG
                     INEEGGPSPREGRVVGIRTQAHDLGTEGAGTITRGNAAGAIGRIVHLAFPLYFMKTEQ
                     ARQLLQTSFNYVDQSPTLP"
BASE COUNT         3644 a         7412 c         7141 g         3635 t
ORIGIN      
        1 gaatctgcgg agcgcagcca tccgggtcac gagcggtccc gcggacgact cgcacccgcg
       61 attctcggcg gacgggaaca ccatctactt catctcgaat cgaaccaaca actacggcct
      121 gaacggcgtc ttcagcaccg agcgtcgcgg ctacaacatc tggggcgtca cgcgatttga
      181 ccgtccctga ggcaaccgat cgagggcccg gaccgaagcg cggccgggcc ctctttctat
      241 cccactgggt ccccgtggcc ctctatgtgt gcctcatctt cgggatgtcc tcgattcggg
      301 ggctccaggt cccgggcccg ttcccgttca tggacaagat cgcccacttg atggagtatt
      361 cgctgttcgg gctcctcgtg ggacgagcca tccgcttcac gatgtcgggg tcgagccgcg
      421 tgacgatgtc gatcgcggcg atctcgttcg gggcgttcgt cgggatgtgc gacgagatct
      481 atcagcgctt cgtgcccgga cggagcagcg acgtcttcga ctggctgacc gacgtcaccg
      541 cggtcacctt ggcggtcgtg atcacgcagg tcgtgagcgc gcggcccctc ggccggcgcg
      601 cggcgccgga acgctgagtg cgtttccaat ccccagattc ccgattcacg acgaaggagc
      661 cgagtggcca gtaagttcca ggtgccccgc ggaacgcggg acatcctgcc gggggagagc
      721 gagcgctgga tcgcgctcga ggcgacgacg cgcgagatcc tgagccgcta cggctatcgc
      781 gaggtccgca cgccgctctt cgaatcgacc gacctcttcg tccggagcgt cggagaatcc
      841 accgacatcg tccgcaagga gctctacacc ttcgaggacc ggaaagggcg ctcgctgacg
      901 ctccgccccg agggcacggc cccgctcgtc cgggcgtacc tcgagcacgc gctcggccac
      961 ggcgatccgg tgacccgcct ctactacatc gggccgatgt tccggtacga gcgcccgcag
     1021 gcgggacgct accgccagtt ctggcagatc ggtgccgagc tcttgggccc cgccgctccg
     1081 gccgcggacg cggagatgat cgatctcttc gtcgcgatcc tccagggcat cgggcttcgc
     1141 gacgtgcgcg tgctcgtgaa ctcgctcggg gactcggtct gccggcccgt gtatcgcgag
     1201 cgaatccgcg agcacttcgc ctcgcgcgag gcggagctct gcggcgactg caaggagcgc
     1261 ctgcgcacca atccgctccg catcctggac tgcaaggttc cgtcgtgcca gccggcgatc
     1321 gcgtcggcgc cgagcgtgct cgactcgctc tgcgatccct gccgcgagca cttcgagggc
     1381 gtgaagaagg cgctcgacgc gctctcgatc gagcacgaga cggcgccgcg cctggtccgc
     1441 gggctcgact actacacccg caccgtgttc gaggtgcacg ccgcgggcct cggcgcgcag
     1501 aacgcggtgg gcggaggcgg gcgctacgac cagctcgtca aggatttcgg cggcccggag
     1561 acgccggcca tcggcttctc gatcggcatg gagcggctcc tcctcgcgac cggaagcttg
     1621 gagaccggtt cggcgctccg cccggacgtc tgcgtcatcg cgcgcgagcc gggagcggtg
     1681 ctcgaagcgc tcaaggccgc gcgcgagctc cgtgggctgg aggcggacgg gcgcgtggcg
     1741 gggcttcgcg tgctcgtcga cgtggccggc cggagcacgt ccgcccagat gaagtgggcg
     1801 tcgaagctcg aggcccggta tgccgtcttc gttccgcccg attccggcgg ctacgccgtg
     1861 cgcgacctgg aagcggggca ggacgcgccg aagcatgcga ccttgcctca actacgagcc
     1921 tggctcaggc agcagaccgc gaccgtttcg gaggacgtga tccgatgatc gagccgtttc
     1981 gcgcccggaa gcgcacccat acttgtggcg acttgaggcc cgagaacgcc ggcatgaagg
     2041 cgcgcctcct gggctgggtg caccggacgc gggatcacgg cggcgtcctc ttcctggacc
     2101 tccgcgaccg gtacggcatc acgcaagtcg tcttccgtcc cgagcacctg ggggacgagg
     2161 cgatggagcg cgcccgctcg atcgggagcg agtacgtggt gatggtcgag gggaaggtgc
     2221 tgaaccggcc gcgcggaagc gagaaccagg atctccccac cggcgggatc gagctcgagg
     2281 cggaggcgct cgcgatcctg aacacgagca agaccccgcc cttcccgctg gacgagaccg
     2341 cgacggcctc ggaggacctt cgcctccgct atcgctatct cgacctgcgg cgcgaggcgc
     2401 tccagtcgag cctcatcttc cgccacaaga tgacgcggag cgtgcgcgag catctctccg
     2461 atcagggctt cctcgagatc gagacgccca tgctggtgcg gccgacgccg gaaggcgcgc
     2521 gcgactacgt cgtgcccagc cggctccatc cggggaagtt ctacgcgctg ccgcaatcgc
     2581 cgcagctcta caagcagatc ctcatggtct ccggcttcga ccgctatttc cagatcgcgc
     2641 gctgtctgcg cgacgaggac ctccgcgcgg accggcagcc cgagttcacg cagatcgacc
     2701 tcgagatgtc gttcgtcacc gaggacgacg tgttcgagat cgtcgaaggg atgctcgcgt
     2761 cggtcttccg gcgcgacttg aagcgcgatc tcaagattcc attcccacgc ctcacctacc
     2821 gcgagtcgat ggagcgctac gggagcgaca agcccgacct ccggatcccg ttcgagatca
     2881 aggacgtcac cgacgccgcg gggcagagcg cgttcgagaa gttcaaggaa gcgcgcgccg
     2941 gcgggcctat gctcatgacc ggcgcgctcg cggtgccggc gctcacgcct cccgcgtcgc
     3001 gccgcgagat cgagctctgg gaggcggcgg ggaaggcgca gggcttggcc ggactcgcgt
     3061 ggacccgcgt gaaggggggc gcgctcgaag ggggcgtcgc gaagcagttc ggacccgagg
     3121 ctcagaaggc gctcatcgag gccgcgggtg cgcgggagga ttcgctcctt ctcctcgcgt
     3181 ccggtccgcg ggccacggtg cagaagggac tcggccacgt ccggctccag gtggggcagg
     3241 ccaagttcgc gctcgatgca caggatttcc gcttcgtgtg ggtccatcag ttcccgctct
     3301 tcgaatatct cgaagagcgg aagcagtggg ctcccgcgca ccacatcttc acgatgccgc
     3361 tcgacgagca cctcgcgcat ctgacgaccg atcccggcaa ggtgcacgca cagctctacg
     3421 atctcgccgc gaacggtcag gagctcgcga gcgggagcat ccggatccat cgaaaggaca
     3481 tccaggagaa ggtcatgggg gtgatcggga tgtccctgga ggaggcgcga gccaagttcg
     3541 gcttcctcct cgaggccttc gaatacggcg cgcctccgca cggtggcatc gcgctcggac
     3601 tggaccgcct cgtcgtgctg ttccacggcg gcgactcgat ccgggactcg attgccttcc
     3661 cgaagacagc ccgcgcgacg tccctcatgg acgacgcccc cggcccgatc gacgaggccg
     3721 acctccacga gctgcatcta aacgtgaccc ggaggaagga ctgaccgagc cttcccccgg
     3781 ccggtggtac tgtttctgct ctctcgtgca tggcgcctgg accgggcctt gacttgaccc
     3841 gggcaggtgc ctagggttga ggcgtggcgg ggcaccccga gcgaggggca gaaaacctcc
     3901 taagttgaaa aattcaagag agttgagaga tcgcgattcg aggccggcgg gcgccccaat
     3961 gggggagccg gaccggcggc atgggtttgg ccgatcggtc cgattctggc cgcgcccagg
     4021 ctccgcgatt ctggcttcgc tcgccatggt cctggccgcc gccctggccg gctgcggcgc
     4081 tacggccccc tgcccgaccc cgaccacgga actggacagc ctcaggaccg tggccgaggg
     4141 gctcgagagc caggtagacc gggagaccgc cgaagggcgt accctggagg cccggcgcga
     4201 ccgggaggcc gtgagggcga cgacggccca ggcggcgctc gattcgcttc aaggagtgca
     4261 ctcccattga gggcccgcgg cgtgacggcc gcgctcgcga ttctcgtgac cgtggtcgcg
     4321 ggatgcggga agccggtgct tcgcgtcgcg gacgcgtcct tgggggacta ctacacggag
     4381 aaggagtacc ggaacctgag cgacgagcag cgcgcggagt actgccagga gctgtccgag
     4441 cagcgggacc tctttcgcga gcagatcgag ggggccgagg aagcgctcga cgcgctgcac
     4501 caaaccgtag ggccgctccg cgccgaggtc gattccctga cggcgcgggc ggaggagctc
     4561 gagcggcggc tcgccgaggc cagggcttcc ggaggcacga atctcatgaa gggcggcgcg
     4621 gaagcgagcc gcagcgcggc aagcggcccc gcatcggctc gggagtccac gccggcgtcc
     4681 gaccgccacg tggtgaagcc cggcgagtcg ctctggtcga tcagcgcgaa gtccgacgtg
     4741 tacggagagg gagcccgatg gaatcgcctc taccgaggca acaagaaccg gatcaagaat
     4801 ccggatcgca tctacccagg acaggagatc cagatcccac gatgaactca cccgcggcgg
     4861 aaacgacgaa gtccgtgccc atcccggacg tcgacccgct ctctctgttt ggaaagaacg
     4921 acgcgaatct cagactcgtc gagcgcagct atcccgtgtc gctcacgtac cgggacggca
     4981 agatcgccgt gcgaggccag gccggcgccg tcgacgaagt gagcaccgtg ctccatgcgc
     5041 tcgccgacct cgtgcggcgc ggcgccgtgc tggaggagca cgacgtcaac aatttcatcg
     5101 gattgaacaa cgcggcggac cagaacgcgt cgctcgagct gtacgagcac ccggtcctct
     5161 tctcgttcga caagcgcacg atccgcgtga agaccctcgg ccagtcacgc tacgtcaacg
     5221 cgctccagaa gcacgacgtc gtcttcgcca tcggtcccgc ggggaccggc aagacctatc
     5281 tcgccgtcgc ggccgcggtc cacgcgctca agtcgcggca ggtcgagcgc atcgtcctcg
     5341 tccgtccggc tgtcgaggcg ggggagaacc tcggattcct tcccggcgac atgaaggaga
     5401 aggtggatcc gtatctccga ccgctctacg acgcgctcaa cgatctcctc tcgtacgaga
     5461 agatccgccg cttcctcgac ctgggcgtga tcgaggtggc cccgctggcc tacatgcgcg
     5521 gccgcacgct gaacaacgca ttcgtcattc tcgacgaggc acagaacacg acggtcggcc
     5581 agatgaagat gttcctcacc cggctcggct acggctcgaa ggcggtgatc acgggggacg
     5641 tcacgcagat cgacctgagc acggaccacg cctcggggct cgtcgagatc cagacgatct
     5701 tgacgggagt gcccgacctc tcgttcgtgt acctgaacga gagggacgtc gtgcgccacc
     5761 atctcgtgcg cgagatcatc cgcgcgttcg acgagcacga gaaggcgcag ccgccgcgag
     5821 cccagacgtc ccagggttcc ggccccgcat gagcaccgag cccttaagag tcctcgagtc
     5881 ccgccgccgc gagaagagga acggccccaa ggcgaaattc ctgcgcctcg tcgggcaccg
     5941 ctcgcgccgg acgatgctgc tcttgggctt cctcctcttc acgtgcctcc tgattcctcg
     6001 ggagccggac ttcgaggtca tcaccctccg cgaggggttc ccggcgaagc gcgacgtgat
     6061 cgcgccgttc cagttctatc tgctcaagga ccgcgccctc tatcacgccg agcaggcgtc
     6121 cgcggcgcgc aaggtcccgc ccgtctactc ggtggatcct gccgtcgcgc cgcaggtgca
     6181 cgccgcgctc gactcgctcc tcggcgacgg atcgaagcac ggagccgtcg cgcgcccccg
     6241 cctcgaaggg ctcgggctct cggccgcgac gaaccgcgtg ctcctcggac ccgacggtcc
     6301 gcgtgtcgtc gtgctcgcgc agcgaatcgc cgaagaggcg tacgccgtgg ggctcatggc
     6361 cgagatcgag agcggccgcc tcgagaccga cgcgggcgcc gcctccgtgc agcgggacgg
     6421 catgcgcgtc cccgtggcgt ggggctcgct cttcgacctc gacagcgtcc gcgagaaggc
     6481 ctgggacgac ggggcgcgtg cgttcccgaa ccggccggag gcggcgcgcg cgctccagga
     6541 attgatcgcg gcggtcgtca cgcccaatct caggtacgag gcctcgacca ccgagcgcat
     6601 caggaccgag agccggaacg ccgtcgagcc ctacgacggc ctcgtgcgga aggacgagaa
     6661 gatcatcggc agccacgaga tcgcgacggc gctccacatc cggaagctgg agtcgctccg
     6721 ggcgtggaac gatcgcgcgc ggccgaagga cgcctggagg caggacctcc tggcgctcct
     6781 ggcgcggctc gccttgatct cgttcctgat cgcgggcttc ctccgctacc tgaagctgaa
     6841 ccacgcgcgc gtctatgggg acgtcaatct attgtcgctc ctcgcggtcc tgaccgcgct
     6901 ggtcatgggg gtgggatggc tcgtggtgag ccagttccgc ggttacgaga tgctgatccc
     6961 gatcaccgtc ctctcgctct cggcggcgct cctcttcgga caggcgctcg cattcgcggc
     7021 gacgctcgtc gcgtcgctct tgatcggcgt cgtcttcggg ctcgggctcc cgttcctcac
     7081 ggggagcgcg ctcgcgggga tcgcggcgat ctcgaccgcg cgcggcgtga gacggcgtcg
     7141 cgacttctac cgtcccatgg tggtcatcgc cgcggcctac gcgttcgcga tcatcgccat
     7201 ggggctcgtc gacggcggac aggggatcgt gctccttcgg cgcgggctct ggggcgtggt
     7261 cgccgggttc gcgtcggtcc tcgcggtgac cctcctcctt ccgctcctcg agtccctgtt
     7321 ctcggtcacg accgacatca ccctgctcga gctcgcggac ctgaaccgtc ccatcttgcg
     7381 ccggctgatg ctcgaggcgc ccgggacgta tcaccacagc ctggtggtcg gaagcctctc
     7441 cgaggcgggc gcggcggcga tcggcggcaa ccccctcgtg gcgcgcgtcg gagcctacta
     7501 tcacgacatc gggaagatcg agaaggcgga gtactacgtc gagaaccagt cgagcgcccg
     7561 gaaccggcac gagaaactct cgcccacgat gagctgcctc atcatcgagg cccacgtgcg
     7621 cgaaggtgcg gacatcgcgc ggaaggagcg gctgccgcgc gccatcatcg acgcgatcct
     7681 cgagcatcat ggaacgacgc tgatgacgtt cttctaccac aaggcgcaga acgccgatcc
     7741 ggcggtggag gagcgcgact tccgctatcc tggccccaag ccacgctcga aggagacggc
     7801 cgtgctcatg ctcgccgacg ccgtcgaggc cgcggctcgc tccctgtcgg agcctacgcc
     7861 cagccgcatc cgtggcgtcg tcacgcggat cctggacgcc cgagtggagg acggccagct
     7921 cgatgaaagc ccgatcacgt tcgaagacct cgccaaggtc cgggagagct tcatcccgat
     7981 cctcaccgcg ctcttccacg cgcgcgtcga ctaccccggc acgccggcgt cggagcgcgg
     8041 gcgccgtccc gattcgcgtc gcgttctcgg ggagaagagc cggcttaaga tcgaccactc
     8101 ttaaggcgct cgtccgctcc gcgctcgacc ggctgggcca tccacgcgcc ggcctcgacg
     8161 tgatcctcac cgacgacgcg tacgtgcgta cgctgaaccg ggactaccgc gaggtcgaca
     8221 agcccaccga cgtcctctcg ttcccgttcg cggagccggc gacgctgtcc gatcccgacc
     8281 gagccgtgtt cctgggagag atctacgtgt cgctcgagac ggccgcggcg caggcgcgcg
     8341 cggcccggcg tccgttcgag cgcgaggtcg cgcacctgat cgtgcacggc acgctgcatc
     8401 tcctgggtca cgatcacagg acccgatcgg agcggcgtcg catggcggcg ctcgaggcgc
     8461 gacttctccg cgcgccgctg gtatgatgcc gccgtgttcg ctcgcctccg cacttatttc
     8521 ctgacgggac ttctcgttct ctcgcccgtg gtcctcacgg gctacgtcgt ctggaagctc
     8581 ttcgtcttcg tcgaccacct gctcggcacg accatgcggg gcggctatct ccgcccggga
     8641 gggatcccgg gcatcgggtt cctcacggtg atcgccctga tcctcctgac gggcgccgtc
     8701 gcgaacacgt tcctcgcccg cacgctcggc tcgctcttgg agtcgctgct cctgcgcgtg
     8761 ccgttcttgc gcggcctcta taccattttg aaagagattg gcgaggctct cctgggcgac
     8821 cgccggcagg cgttccagcg ggtggttctg atcccgtacc cgagccccga tctctattcg
     8881 atcggcttcg tcaccacgcc cccgcctccc tcgatggtcg aggcgctcgg ggagccggtc
     8941 gagggggtgt tcgtccccac gccgcccaat cccgcgacgg ggcagctcgt ctactacccg
     9001 aagcggaagc tgatcccgac caacctcaag atcgatcagg cgatcaagat cgtggtctcc
     9061 tgcggcgtgg tcgtgccgtc cgatcccgcg atgagggcct gagcgtggcg atcgtccaga
     9121 ccgacgcgct cgtgctgcgc tcgtaccggc tcggcgagac gagcctcatc gtccacctct
     9181 ttaccgacgc gcacgggctc ttgcgctgcg tggcgaaggg ggcgaggggt ccgaagagcc
     9241 gcttcggcgg ctgcctcgag cccggcgtcc gtctctcggc ggtgatctac cggaaggcgg
     9301 agcgcgatct ccagctcctc tcgaaggccg acatcaccgc gtactggccc gctctctggg
     9361 acgacccgga ccgcttcgcg cgcgcccagg cggtgctcga attcctcgag cgcgccgcct
     9421 atggcgaggc ggaggacgcg acgctcctcg ccttcgccgt ggccacgctg gccgcgatgg
     9481 cggaggcacc cgtccccgcg ctcgaagccg tgttgcgtgg attcgaggtc cgcgcttgca
     9541 cgctcctcgg gtacgccccc gagctctcgt cgtgcctcga gtgccagaag ccgctcggcc
     9601 aaggagggct cttccatccg ctccgcggcg ggctcctctg cggcgagccc tgcggcggcg
     9661 atgcggcttc gttccggatc tccgagcgcg ctcgaaagac gctcatcgcg ctcggcgagc
     9721 gggagccggc ctcgatcggc acgtgggcga tggagcgcta tcccggcccc gagatttcgc
     9781 gcgccatcga gcgcttcctc tcggcgcacc tggagcgctt cgacggactc cgctcgacgc
     9841 gcgtcggcga gcgcctcgcg cgctacgcgg agggcgcatg accgcccagg cgccgcgcac
     9901 gttccaggag acgctcctcc gtctcgagca gttctggtcg gaccagggct gtgcgcttct
     9961 ccagccctac tcgagcgagg tgggcgcggg aacgttcaac ccggcgacct tcttccgcgt
    10021 gctcggcccg gagccctgga aggcggccta cgtcgaaccg tcgcgccgcc ccaaggacgg
    10081 ccgctacggc gagaatccca accgtctcca gctcttctac cagtaccagg tggtgctcaa
    10141 gcccgcgccg gaggacgtgc tcgaggtcta cctcgacagc ctccgccatc tcggcatcga
    10201 cctcgcgcgg cacgacgtgc gcttcgtcga ggacgattgg gagtcgccca cgctcggcgc
    10261 gtggggcctc gggtgggagg tctggctcga caatcaggag atcacccagt tcacgtactt
    10321 ccagcaggcc ggcggctacg acctggagcc aatcacggcc gagatcacct acggcatcga
    10381 gcggatcgcg tgctacctcc agggcgtcga ccggatcatg gacctgcagt ggtcgcgcga
    10441 tcacacctgg gcggatctct ttctccaggc cgagatcgag tggtgcgggt tcaacttcga
    10501 gcactccgat ccggcgctcc tcttcgagct cttcgagaag ttcgagcgcg aggcgtaccg
    10561 cctcctcaag gcgggcatgg tgctgccggg ctacgactac gtggtcaagt gctcgcacgt
    10621 gttcaacctg ctcgacgcgc gcggcgcgat cggcgtgacc gagcgcgtgg cctcgatcgc
    10681 gcgcgtccgg aagatggcgc gtgcggccgc gggcgcgtat ctcaagcagc gcgaagagct
    10741 gggctggccg cttctcaagg gcgccgccac acccgaagcc gcagtcgcga cccgtggctg
    10801 agacgcttct cctcgagatc ggctgcgagg agatcccggc cgggttcatc gcccccgcgc
    10861 tcctcgagct cgagacgctc gcgcgggacg gtcttgcgga cgcccgcctg gcccacggcg
    10921 ccgtgcgcac gctcggcacg ccgcgccgac tcacgctcat cgtcgaaggc ctcgccgacc
    10981 gtcaggagga ccgcgtcaag gagatgaccg gtccggccgt gcgggtcgcg ttcgacgccc
    11041 aggggcagcc gacccaggcg gccaaggggt tcgccaagtc ggcgggcatc cccgtggagt
    11101 ccctcgagcg cgtcaccacg cccaaggggg agtacctcct ggcgcgcgtg aacgagacgg
    11161 gagcggccgc ggccgacgtg atgccggccc ggctcgcgtc ctggatcgct tcgctccact
    11221 tcccgaagac catgcattgg aacggaccgg ggcgcttcgc ccgccccgtg cgatggatcg
    11281 tggccctcct cggcgaccgg gtcctctcag tcgaggcgtt cggcgtcgtc tccggccggc
    11341 ggacgcgcgg acaccgcgtc cttgccccgg gctggctcga cgtcccgtct cccgccgcct
    11401 acgccgcgtc gctcgaaggc gcgggcgtca tcgtggatcc ggacgcgcgg cgcgtgaaga
    11461 tccgcgagga gctcgcgagg accgcggcag ggctcgaggg ccgcgccgtc gaggacgagg
    11521 agctggtgga cgaggtcgcc aacctggtcg aatggcccga ggcggtcgtc gggtccttcg
    11581 agtcccgata tctggagctc cctcgccccg tgatcgtggc atccatgcgt gcccaccagc
    11641 gctatttcgc ggtggagcgc ccggatggag ctctgctccc gaacttcgtc atgatccgga
    11701 gcgggcgcgg tcccggcgcc gaggagatcc gccggggcac ggagatcgtt ctccgggcac
    11761 gcctggaaga cgcccggttc tactgggtca atgatctgaa ggggggcctc gaggggaagc
    11821 tcccggtcct caagggaatc gtggtgcacg agcggctcgg gacggtccac gacaggaccg
    11881 agcgcctggt ggtgctcgtg gaagacctcg cgaagaagct cgccccgaag tcgctcaccg
    11941 ccgcgtcgcg ggctgcccgc ctctgcaagg cggatctcgc gagcgagatg gtccgctcgg
    12001 ggaaggagtt cgcgtcgctc caggggatca tcggcggcga gtacgcggcc gcttcgggag
    12061 agccggcgga cgtcgcggag gcgatccgcg agcactaccg ccccgaaggg ccgtccgatc
    12121 ccattccgag gaccccggaa gggaagctcg tggcgctcgc ggacaagatc gaggcgacga
    12181 ccggcggtct gcgggccggg ctcgaggtga ccggctccca ggatccgttc gggctcaggc
    12241 gcgcaggcaa cggcatcgtg cggatcctgg tcgagtccgg atggcgcctc gacgtcctcg
    12301 acacagctcg tttcatggat gggatttacg acaaggcagg agtcccgccg ggggatccgg
    12361 accgctttgc cgagttctgg aagcagcgag tggcgagtgc gctggaagta tatgagattt
    12421 cgtcagaaac ggccggcgcc gtgctggccg tgcgccccgg ggatccgatc gacgtgctgg
    12481 cgcgggccag ggccatcgag gcgatccgga agagccccga cttcgagggg ttgatgatcg
    12541 gctatcgccg cgctgccaac atcttgcgct cggcgccacc ggccgacatc ccggcgtcgg
    12601 ggcagcccct cgccgagcga gccgaggcct tcggcgaccg ggccgaggcc gatcttcacc
    12661 tcgaaaccaa gatggcccgc caggccgtcg agacctacct caagggggcc ctccccgact
    12721 acggagtcct cctcaggcac ctcctggggc tccgtccgtc catcgaccgc ttcttcgacg
    12781 ccgtgatggt catggtggac gacccaatcg cccgcaaacg acggctggga ttactcgaag
    12841 cggtgcgcca aaccttcctc cgaattgccg atttctccct gttgccaagc gctccaggtc
    12901 aaaaatccaa ttgacattag cggcttttcc gcctatactg atcgagcaaa ggggacaaaa
    12961 taacttgtcc cttggaagcc gcaaccgcta gaacctattg actttacacc tgacgaacga
    13021 caacacgtgg gctgacggtg cggctcattt tgtctttggt cgggaactta ctgcattgac
    13081 agcatagaac ggcattacga acgaggaggt gaggcgactc aggtccacgt ttacttcttg
    13141 aaaacagaag agttagaagc cctgaatcca aaaaacgata ggaaaggagg tcggtaaaag
    13201 cgagagggag gtgccccaag agctcggtcc ctgtcccaac gagcaggcgc ctcgaagctc
    13261 cactccaagc tcaattgtca gtaccgaatt ccgtgaaggc aggttccaaa gcaagtcctc
    13321 ccgaaattgg actccgtgtt aggaaacgct aaggaggatg tgaaaatgcg gaaaacctta
    13381 tttgccatgc tgggcctcgt ggcgattggt ctgtgggcca tccccgtggt cgcggcaccc
    13441 agcgggctag tctcggacgg gccgagcaag gtccgtaccg accccgttcc gctcgatgcc
    13501 cgggctggcg cggtcggagc agccgcgcgt ggcgaacgct acgcggggac gctgagcggc
    13561 tcgctgatca agcagtccgc cgtgacgacc aactacttcc tgtatccggg tgcctgcgtg
    13621 cagcgtgcgc ttgggacctg ggcggcgaag acgagtccgg cggcggacag cctccagccg
    13681 aacgccagct tcccgaactc gagcggctac acggctggac agcccaatcc ggccagcaac
    13741 acgatcgcct acacccgtac cgacctctcg ctgccggaga agctctggca catcgagtcg
    13801 agctccacgc cggccggtca gcgccccgcc atcctcgatg gcacgcgcat gatctggtgc
    13861 ggcaagttcg acgcgaactg gacggacgat gtcggctatc cgaacctgac cttccagatc
    13921 ctctacatcg acacgggtac gcatgcgaat ccgggcggca agtaccacct taacttccag
    13981 gggaacttca gctcggagct caattacgac tacgtgacct tcatcggtgg cggctccggc
    14041 ggtgcgctgg atccgatcgg gaacagccgc atcttcttga gcgacatgct tccgccggtc
    14101 ggcagtccgc atggcgggcc gggcggtgac tccgatcagc tcgtctcgtt cacgggcgcc
    14161 atccgcacgt cgcagaccat cagcatgaac tccggtgcgg atctgcagat cattacgggc
    14221 gatgcctcgc tcaatccggt cacgcagagc tactccctcg aggtcgacga cgaccatcgc
    14281 ggcatgtacg tcgtcttcac cgcggactgc ttgttctcgt ccgaggacgg cctctggccg
    14341 aacagcgatg gccagtcgat ggatgacatc gaagtcgaca acaacggcgt ggtctcgtcg
    14401 atctacaccg acgccgccac cttcgtgccg ccggctggcg tcagcgatca ggacgcgtcc
    14461 aacggcaccg ttctcgtgtg gtccggcaac gtcatcccgc aggctgggga cgacacgccg
    14521 attccgatca tttcgtcgcg tgtcccgccg ggcgtcggcg agcgctggca gctgtcgacg
    14581 ggcgcgtcca acgtgaccca ggacgtctgc tctccgcaga aggccctggc gaacgacctc
    14641 ttcttcgagg gcggcgatct cgcgacccac acggctatca acaagcagta caacgccatc
    14701 gttccgtgcg tcttcccgat tcctgccgga agcgcgcagg tgctggtgtt ctggaatgag
    14761 tatctcgaca tcccgcggtt ctcgggctac gtgcagcagg ccgagttccg tatcttcaag
    14821 ttcggggctt ggggccagtg gaacaacacg gctccgggtg gtggtgtgac gaccggcgcg
    14881 aaccaggcct ggttggtcga tggagacgac atctccacgg cgacgcaggc ggactcggtg
    14941 cagctccggt tcgagctcca gtgcatcccg ccgttcgcgg cagaccgtgc caactgcgcg
    15001 accggccaga tcaacccgat tctgtatgac gacctccgcc tccaggtggt cacgggcgtt
    15061 ccggccccgt tcttcgggat cttcccgggt tcggtggcgc agacgacgtt cgtcgacggc
    15121 cggatcggaa ccgccggcat cggacaaaac ctgaccaact gcaccgcggg caacctgagc
    15181 gcgggtcagt gctggccggg caaccgtggt tccggtctcg gcacgcagca gtcgcacaac
    15241 attgccgtca acaacaactt caatgctccg gtgggtgaca cgatcaccct gtcgctcttg
    15301 accggtcttc gcaagaacgg catgggcatc aactggaagc atggcttcaa caagtcgatc
    15361 aacgctggtg aattcggacc tccgggcagc gagtacgcgc acacgaatcc gacgttcaac
    15421 gcgaactgcg acatgcctcg gatgatcttc cggctcttcg acccgtcgtc caagacctgg
    15481 tccccgtggg attccacgga gctggtcgcg aacgcggttg cgccgggacc gacggacacc
    15541 acgctcgtcg actctgagtt caacatcgat tggcctccgt acgacaaggc gatcgcgaac
    15601 gccagcatcc ccggtggctt cacgatcaac ggcgtcggca actacaacgc cctttcgttc
    15661 ctcccgcgcg gcgcgcggat gcagtactac ttcaaggcgg ttgacatcgg taacggctcg
    15721 ggcgctggat gcggcgtttc gtaccagttc tcgtccgaca acctcgcccg cgaagtggaa
    15781 gacatcccga cgctgccgaa cagctccatc aaggcgccgg acatcatcga gttccgagtt
    15841 ctcccgagcg tgtacccggc tggtcccgcg ggctcgctcc tggccggccg caccaacacg
    15901 ccggtgctga acctcgacgc ggtctacacg acgtgggcgt tcggttacga cccgatgacc
    15961 caggcgctcc gcgctctggg cgtccgggcg gatcggtacc gtcacacggc ttccggcacg
    16021 acggctcatg ggttcggtgg tcacgagctc ccgggcaagc gtccggaccg tctgagcaac
    16081 ttcttcccga actacctcga gtatcctctc gtcgactcgc tcgcgtcttg gtaccggatc
    16141 gtcatcgagt ccggccatac ccgcacgagc tcggtgttca acgaggaaga tgcgttcacg
    16201 ctcgagcagt ggtggcggaa ggaaactggt acgaacggtg gagaccgctg cgcgttcctg
    16261 agcggcgacg accagatgta ccagatgatc acgccgagcg gcgtggatgc gacgttccag
    16321 atcagcatgg ccggtcaggt gttcggtgtc gcttcggccc agaacagctg gtccccggct
    16381 acgtcgtact tcaacatgga tgaccgcttc gcggctgcga ccgctggacc ggctctcgcg
    16441 gcgcctaaca ccttcgtcta tccgatcgac ggtgggtgcc cggccccgaa ccggttcgat
    16501 gcgctcaccg cggctggtcc gtcgggagat acgccattca tcgccgccgt gtatccgagc
    16561 gggcaggcgg ctgctgtcgg ccgcatgcgc gagctggaca acatcggtga caatgatcgc
    16621 aacaaggcga tcgcctacgg cttctcgctc cagttcatcc gcgacccggc gatcagcaac
    16681 acgagcgcga actacacgcg ctctggtgtc gagaaccgca tgcgcgtgat gtacaagttc
    16741 ctcacgagct gccgtacggc tcaggtcggt ccctcgacct gcttcccgtg cccgaatccg
    16801 accccgcaga ccatcgcctc gatgcaggcg gattggtcgg gtcagaatgc tgggttccag
    16861 accgccacct acggttccct ctacccgatc caggccggcg ctctcgcgac ggcggtcggc
    16921 gaggatgctc cggaagcggc gcctcggatc aacaagatcg acggcaacta cccgaacccc
    16981 ttcaacccgc acaccgcgat ccgcttctcg agcgcgcagg cgggcaaggc ccagatccgg
    17041 atcttcagcg tgagcggcgc gctggttcgc acgctgcaca cgacggtcac ggctggtccg
    17101 aacgaggttc ggtggaacgg caagaaggac gacggcgcgt cgctggcgtc cggggtgtac
    17161 ttctacaaga tcgtcttccc gaacggggag tcgttccgtg caccgaacaa cctggtgctc
    17221 gtgaagtagc ccaaagcgtc acgcagtagc gagagggccg ctgggaaacc agcggccctt
    17281 ttcgttttcc tttgcggagc tcggtagggc gatgcggggc ctttgcgtgg attggccggc
    17341 gaggggcggg tgtgggggtc cgggacagca catgccggcg ctcgcagtcg gaccgctccg
    17401 gcgacttggt gggcgccggc tgcggatggt cccggacccc cacacccgcc cctcgcctcc
    17461 caaggcgcgc aaagcgccgg gtcgcccttc gggctccccg gaagggtacg aaaggggagg
    17521 gaggtagccc agtggcgccc ccttcttgtg gggaaggagg acccggggca tgtgggggct
    17581 cttccagggg tcggagagcg gggcggtggg ggtggtgagg tcggaagcgc cctcgcagtc
    17641 cggccgcccc aaaccgctgt gaacttgaaa gctagcacgc cggacatgcg ccggcgccgg
    17701 agacctcacc gcccccaccg ccccgccccc ctaccggggg aaaatgccgt tgacaccacc
    17761 cccctgggac ggtaatttca cggcagtccc caactcgcac tcagacacct gccggtggtc
    17821 aaccaagtct cctcggagaa cgaaatgcta ttgccaagac tttggcgcat tacggccttg
    17881 gtcgttttca cgctcgtgac cggaatcgtc ctcttgtcct gtgcgaagga cccgcttcga
    17941 gtggatcgca accgaccgcc gcagaccttt ctcgtcgcag cgcccgtcga cacgagcgtg
    18001 gccgacatcg cctactccta ccggatccac ctctactggc gcggcgagga ttcggacggc
    18061 tatgtggtcg gtttcgagta ctcgtgggac gactcgagca tcggagcgtt caagttcacc
    18121 accaagacgg actccatctt cgagctgacc gtgaacgaca gctcgcagat ctcgggagga
    18181 accggccaga atccatcgac ggcgcgcacg cacacgttct acatccgcgc cgtcgacaac
    18241 ctcgggaagc cggatccttc gctcacgatc ttcaatcgcc gccccttcaa ggctcagacc
    18301 gacgcaccga tcgtgacctt cacgggcgcc attcccaatc cgcaattccc ggccgtgctc
    18361 gatacggtct gtgaaggcgc gccgtttact gtgagctgga atgcgcgtga tcccgacggc
    18421 acggtgacca agtatcggtt cgacgtgggc gcctatcaca gtcctttcac gaccgactcc
    18481 tcggtgacct tcaacgatcc gagcgtccct ggctcggtgg acctcccgag cggcgtctac
    18541 acgatgacag tgaccgcgat cgacaacgcg aatgctgttg ggacgggaag cacgcagttc
    18601 atcgtgaacc acgatcccga gactgtgctc gcgcgtagcg acgcccgaca tctggtgggt
    18661 cactacatcc agcacttcct gggcggccag caggtgaaca tcgagggcac attcaccttc
    18721 gacccgggtg acccggccgg cgggtccgct acggtcccct atcgctccac ggtatggtgg
    18781 gagtgggacg gcaatgattc gcacgacggc tgcgaggccg actgcctcag cggcttttcg
    18841 atcgtgctca atccgggagg gcacaacgag aacaatccgt acatcgtcgg cttcacggat
    18901 tccatcggca cggatcccag tggcaacacc gtccacttca agaccaacaa cccggcggtg
    18961 ctcagccagt acggcttcga ccagctcatc ctcgattcgc tggatgccgg caggaacttg
    19021 acgatcagag ttgcggctcg cgactgctcg ggccgcgcgg acgggacgcc tgcgtatttc
    19081 cagttcaatt gcaacttcgc cccgaccctc gattcggtga ccgtgacgcc tacgatgttc
    19141 gacttcgacg gcaatggtcc cgacccagcc gagccggcca ggatcatccg gtgggtcggt
    19201 ctcgactacg aggatggaag gtcgaggggg gcagtggtca aactcgacga tacgcggttt
    19261 ttgactttcg agaactacga ggatttcacg atcgttgccg accgggactt ccaggtattg
    19321 agcacgggaa cgacgcacac cgtcaaggtg cgcgtctcgg accgcgccgg ctacctatcg
    19381 gatcttccgg agacagaagt catcttccaa attcccctga ctccatgacc cccgccgaac
    19441 gaaggagtaa tgggctgatg cgtcgtttca tggttctgac agggctcgcg gccgcggtgc
    19501 tcgggctcct ctatctcacc ggctgcgggg acaaggtcac gccaccgccg ccacaccagg
    19561 atacgaatcc cgagacggag ctcacctacg ctccgctgcc cggagacacg accgcgttcc
    19621 gcgtccattt ctactggaac gggtacgaca aggacggcga ggtgatttcg ttctccttcg
    19681 cggtggacgc agacaccctg aaggatccga aggactggcc gacgaccacg tccaaggaca
    19741 cgacgctcct cttcctggtg gaccccgtga gggaagttcg ccagcacgtg ttcaaggtgg
    19801 cggcccgcga caatgacggg cagtgggatc aaagccccgc gttccggttc ttctcggcga
    19861 agaccctccc gccgacgtcc ttgatcaagc gcggtcccgc cgcgttcaat ccgattgtgg
    19921 ggccgaactt cacgttcgaa tgggaaggca tcgatccgga cggtagtgag accggaggga
    19981 agacgcccgt cgactcgttc gagtacgtgc tcctcctgat cggcgcgact ggcgcgccgg
    20041 acgcggacgt gctgccccaa tataatcagg acttctatct gaatctggtc aaccagagca
    20101 cggggcccac gctcaagagc gacttcgaag ggctggggag gatctggaac ttctccgact
    20161 ggcactggat tggaattcac tcgctcaaga accggttccg caacgcgaca cccggcgagt
    20221 acgtcttcgc cgagcgcgcc gtcgacgtgg cgggggccac ggagaaggat ctgggctttg
    20281 gaaccaactt ccggcacttc acggtgagca ccaagaatcc cggtcccagc ttgatcatct
    20341 cgtcgagcgt cctgacgctc cctcttcctg ctgccgtcgg gccgggagat acgccccgaa
    20401 agccgctcca gatcttcgaa ggggagacga tcagcttctc ctggacggcg agcgcggacg
    20461 cctacggcgg cgagatcgtc ggctacacct acgctctcga cgatacgagt acgttccccg
    20521 ggctcgatct cctgcgcacg ggcgcgacct tcaggcccgc gcagctgccg ccgggcaacc
    20581 acttcctgtt cgtccgcgcg gtcgacgacg gtggccttat caccaacgcg gtcatcccga
    20641 tgaagatcgt gcatccggac ttcaaggatc ccggccatcc gcacagcatc gcctacgtcg
    20701 acgattccac gcctccgggc gtgacgattg acaggacgca ggcgatcggg aatttcccga
    20761 gagacctcga cgaggacaga ttctgggagt ccgacccggg aatcctggtc gggctggcgc
    20821 ccttcaacca gtgggacgcg tcggacaatg gctatgggga ggtcgagggc cgaaaaccgc
    20881 cggatccgga ctggctcaag gactactcga cggtcatctg gaacgtcgac ttcaacaacg
    20941 gggtcgggaa tccgaccgga ttgtggcgga cactcgtggg tggcgcgtat agcgagctcg
    21001 ccgggtactt gcgcgcgggc ggcacgatga tcctgagcgg atttttgatc ggcggaaacg
    21061 tcatcaatcc caacacggtc ctctacggca acgtgtctcg aggcatctgc ttcgcgctgg
    21121 agccgggcac gaccgaatac ttcctgggga cgttcccgcg catgttcatg ggcgttgacg
    21181 gcgccgtcct cggcgggccg gatctgcgga cactgggagg gaaggacttc attcaggccc
    21241 gcccgacccc cgccggcatc gcgatggggt tcgacacggt ggacgtcgat cgaggtcctc
    21301 tcggaagcgg cgcgaagtgg atcacctact cgggcttcgg ctccttgaac gagaacctcg
    21361 ccccgggcct cctctatgtc gacggtctgg tcatggcccg aaacttcgcc tgcgtggacc
    21421 agccgaactc ggtcttcagg ctggaggacc cgaccacccc gatcgcggcg ccgatctaca
    21481 cctaccacgg cgtccatagc ggcattaacg aggaaggcgg tccgtcgcct cgggaaggcc
    21541 gtgtggtggg gatccggacc caggcgcacg acctcggcac cgagggagcc ggcacgatca
    21601 cgaggggcaa tgcggccggc gcgatcgggc gaatcgtcca tctggcgttc ccgctctact
    21661 tcatgaagac cgagcaggcc aggcagctcc tgcagacttc cttcaactac gtggaccagt
    21721 cgccgacctt gccgtagcgt cggcgttctt gcggcggccc cggtggatcg catctcccac
    21781 cggggccgtt ttcttttgcg gcctcgacgc gcgtgaatgg gccggcgtgg gg
//
LOCUS       DASTKC010000145         3221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1529895, whole genome shotgun sequence.
ACCESSION   DASTKC010000145 DASTKC010000000
VERSION     DASTKC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529895"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2258)
                     /locus_tag="VFR25_05845"
     CDS             complement(<1..2258)
                     /locus_tag="VFR25_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9940603.1"
                     /translation="MPRRKPDRPSKTPDTRPNPATPDTRPNPAQPDRPTDESSSTRFQ
                     IGAKLGEGGMSQVVGAIDLRLGRPVAIKTLRPEFASDPQRVARMKREARALGKLHHPN
                     IAMIYSSEVNHRGERYLILERVDGESLADRLKHGPLDVATALRIGAQIASALEAAHQR
                     GVVHRDLKPANVMVTPQGVAKVLDFGLAKITHTDDEVSSDTVEIAGVAVGTPGYMSPE
                     QVRGLGEDRRSDVFGFGCTLFECLAGARAYGGTPRHAMEVVLRRDPNWSILPLDTPES
                     VRDLLRSCCERDPAKRLQDMAVARDVLTRASAELDLAAGRPARLDESTRRLTSRMLPD
                     PGTSFVGREREVRDLAGLLRKNRLVTLTGAGGAGKSRLALALAHEAQAEMKDGVWWID
                     LASVMDAERVPSAVASTLGLREQGGVWILDTLEDYLASRSMLLVLDCCEHVLGACRGF
                     AEVLLPRAPDLKILATSREPLKVPAEHVYRVPPLAESVNLFLERVAMVRPDFEPSARE
                     RSAIEEICRRLDGIPLAIELAAARVRVLSIEQIREMLLDPLRLLTAGAPSLEARHQTL
                     AATIGWSYDLLQTHEQRFFRALSVFVGGWSLESASEVARDAGDEFEVLDLLSRLVDKS
                     LVVTEPGGGERLRFRMLDTMRQYALGRLRAAGEERAARLRHLDYFIGLSRRSMAGLVG
                     PEQGAWLDRLEAEHENLLSALWCCGQVEGGAISALRIVGPLWRFWFGHGHFELGRSVL
                     ATELNLPGAKAPT"
     gene            complement(2319..>3221)
                     /locus_tag="VFR25_05850"
     CDS             complement(2319..>3221)
                     /locus_tag="VFR25_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET9940604.1"
                     /translation="ARGAAGAPGAAGDAVAGPAGAAGAAAAAGPAAAASPAATAGPPA
                     LANHVVVAGYGVNGRNVARVLRELGIPYVVLELDTAIIRRARAAGESIQFGDISRAES
                     LHASGVHKASVVVLAISDPSATRFAVHQVRTVNPDAHIIARTRQVAEVAELNRLGADE
                     VIPEEFETSIAILVRVLRRYHVPANVVRIQEQALRREGYSFLRGGDITGQLTTSISQM
                     IAGATTDTFYLEPGSPAIGQTLASLDLRGRTRALIIAVVRDGAHTLGPGPEYELRPGD
                     ILVLVGDHEALDAAFEALSPPRSG"
BASE COUNT          493 a         1134 c         1129 g          465 t
ORIGIN      
        1 gtcggcgcct tcgcaccggg aaggttcagc tccgtcgcga gcacggagcg gccgagctcg
       61 aaatggccgt gcccgaacca gaagcgccaa agcggtccca cgatccgaag cgcggagatg
      121 gcgccaccct cgacctggcc acagcaccag agcgccgaga gaaggttctc gtgctccgct
      181 tcgagccggt ccagccacgc gccctgttcg ggaccgacga ggccggccat cgaacggcgc
      241 gagagaccga tgaagtagtc gagatgccgt agccgcgccg cgcgctcctc ccccgccgcg
      301 cggagcctgc cgagcgcgta ctggcgcatc gtgtccagca tgcggaagcg gagccgctcg
      361 ccaccgcctg gctcggtcac gacgagtgac ttgtccacga gacgggacag gagatccagc
      421 acctcgaact cgtctcccgc atcgcgcgcc acctcgctcg ccgattcgag cgaccacccg
      481 cccacgaaca ccgagagcgc gcggaagaag cgctgctcgt gcgtttggag caggtcgtag
      541 ctccacccga tcgtggcggc gagcgtctga tgccgcgcct cgaggctggg cgcgcccgcc
      601 gtgaggagcc ggagcggatc caggagcatc tcccggattt gctctatcga gagcactcgc
      661 acgcgcgcgg cggccagctc gatcgcgagc ggaattccgt cgagcctccg gcagatctcc
      721 tcgatcgcgc ttcgctcccg cgcactcggc tcgaagtcgg gacgcaccat cgcgacgcgc
      781 tcgaggaaga ggttcacgct ctcggcgagc ggcggcacac ggtagacatg ctccgccggc
      841 accttgagcg gctcccggct cgtcgcgagg atcttgagat ctggagcgcg cggtaggagg
      901 acctcggcga atccgcggca ggcgcccagc acgtgctcgc aacagtcgag cacgaggagc
      961 atcgaccggg aggcgaggta gtcttcgagc gtgtccagga tccagacgcc gccctgctcg
     1021 cgcaacccga gcgtcgaggc gacggcggag gggacgcgct ccgcgtccat caccgacgcg
     1081 agatcgatcc accagacgcc gtccttcatc tccgcctgag cttcgtgtgc cagcgccagc
     1141 gcgagccgcg acttccccgc ccctcccgcg ccggtgagcg tgaccagccg attctttcgg
     1201 aggagcccgg ccaggtctcg aacctcgcgc tctcgtccga cgaagctcgt gccggggtcg
     1261 ggaagcatgc gcgacgtgag gcgtcgcgtg gactcgtcga gacgcgcggg acggcctgcg
     1321 gccagatcga gctccgccga cgcgcgcgtg agcacgtccc gcgcgacggc catgtcttga
     1381 agtcgcttcg ccggatcgcg ctcgcaacac gagcggagga gatcgcggac ggactcgggc
     1441 gtgtcgagcg ggaggatcga ccaattcgga tcccggcgga gcaccacctc catcgcgtgc
     1501 cttggcgttc cgccgtaggc gcgagctccc gccaggcatt cgaaaagcgt gcatccgaac
     1561 ccgaagacgt cgctccggcg atcctccccg agtccgcgca cctgctccgg gctcatgtac
     1621 ccgggggtcc caacggccac gccggcgatc tcgaccgtgt cgctgctcac ctcgtcatcc
     1681 gtatgggtga tcttcgcgag cccgaaatcg aggaccttcg ccacgccctg gggggtgacc
     1741 atgacgttcg ccggcttcaa gtcacggtgc accacaccgc gctggtgcgc tgcctcgagg
     1801 gcagacgcga tctgcgctcc gatccggagc gccgtcgcca catcgagcgg tccgtgcttc
     1861 agccggtcgg ccagcgattc tccgtcgacg cgctccagaa tgaggtaccg ctcgccccga
     1921 tggttcacct cggagctgta gatcatggcg atgttgggat ggtggagttt cccaagggcg
     1981 cgcgcctcgc gcttcatgcg cgccacgcgc tggggatcgg aggcgaattc cgggcggagg
     2041 gtcttgatcg cgacggggcg tccgaggcgc aggtcgatcg caccgacgac ctggctcatg
     2101 ccgccctcgc ccagcttcgc tccgatctgg aagcgcgtgg acgacgactc gtcggtcggc
     2161 cggtccggct gagccgggtt cggtcgcgtg tccggcgttg ccgggttcgg tcgcgtgtcc
     2221 ggcgtcttcg atggccggtc tggtttccgg cgcggcattc ggcctccccc acggatgccg
     2281 aaacgtgcgc gacgcgaggg agcgccgcgc ggaatggctc agcccgagcg cggcggactc
     2341 agggcttcga aggccgcgtc gagcgcttcg tggtcgccca cgagaaccag gatgtccccc
     2401 ggccgaagct cgtactcggg gccgggcccg agggtgtgcg caccgtcccg cacgaccgcg
     2461 atgatgagcg cgcgggtcct tccgcgcaag tcgagggatg cgagggtctg cccgatggcc
     2521 ggagagcccg gctcgaggta gaaggtgtcg gtcgtggccc cggcgatcat ctgggagatc
     2581 gaggtcgtga gctgtcccgt gatgtccccg ccgcggagga agctgtatcc ctcccggcgc
     2641 agcgcctgct cctggatgcg cacgacgttg gccggcacgt ggtagcggcg gagcacacgg
     2701 accaggatcg cgatcgaggt ctcgaactcc tcggggatca cctcgtccgc tccgagccgg
     2761 ttgagctcgg ccacctcggc cacctggcgg gtccgggcga tgatgtgcgc gtcgggattc
     2821 accgtgcgca cttggtggac ggcgaagcgc gtcgcggagg gatcggagat cgcgagcacc
     2881 acaaccgacg ccttgtgcac gcccgacgca tggagcgact cggcccgcga gatgtctccg
     2941 aactggatcg actctcccgc cgcccgcgcg cggcggatga tcgcggtgtc cagctcgagg
     3001 acgacgtacg gaatgccgag ctcgcgaagc acacgggcga cattgcggcc gttcacgccg
     3061 tagcccgcga cgaccacgtg gttcgcgagg gcgggcggtc cggcggtcgc ggctggtgag
     3121 gcggcggcag cgggaccggc agccgcggcg gcaccagccg ccccagcggg accggcgacc
     3181 gcatccccag cggcaccagg cgccccagcg gcaccgcgcg c
//
LOCUS       DASTKC010000146         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_347694, whole genome shotgun sequence.
ACCESSION   DASTKC010000146 DASTKC010000000
VERSION     DASTKC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347694"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2885)
                     /locus_tag="VFR25_05855"
     CDS             complement(<1..2885)
                     /locus_tag="VFR25_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HET9940605.1"
                     /translation="MKLIDGTPIRLSATDLANHVACRHLTAQNRAVVEKRLAPPPRRA
                     DLTLLRDLGDKHERAYLTHLEAQGLRLSRAPQDHVEPETEAVERTLAAMKDGVDVIVQ
                     AALVEGRWLGRADVLRRVERSSIFGSWSYEVYDTKLALETRGGSVLQLCLYSDLLAGL
                     QGVSPEFMHIVPPRPEFPPESYRVADYAAYYRRVRRQAEEAVDSPADDAAGSPTYPLP
                     VAHCDICTWWKSCDDRRRADDHLSLVASISKLQTRELESRGIKTLAGLAGEPIPLSWR
                     PKRGAKETYERAREQARVQLEGRRTGDPVHEWLPLEEGRGLTLVPRPSPGDVFLDLEG
                     DPFAGQGGQEYLFGWAALDETGDPMHYFLWALDRSVERIAFEVFIDEVMARWQQYPEM
                     HVYHYAAYEPAALKRLMGRHATREAEIDGMLRAGLFVDLHTVVRQSMRASVEEYSIKK
                     LEPVFGYARTQSLLEAGVHLRAVQRALELGSPDSVDKPTRKIVEAYNRDDCMSAMSLR
                     DWLEHERTRIESGGVAIPRPIARKTEVSEELGERDARARHLTNTLLTGMPADPDRRTP
                     EQRAIYTLANLLEFHRREEKAPWWEYFDLQRKSDEELMEESAGLSGLEFVERVGGTAK
                     APIHRYRFPKQETSIRADHKLHAPLPDGREVGDVEVIDLAAGTVDIKKRMLFAEEHPR
                     AVFSHKMFSAPKQAESLMRLGGWVADHGLTAPGPFRAARDLLLRLPPRVDGGAGGDLR
                     LPGESGIDAARRLVVSLDTTVLGIQGPPGSGKTVTGARMILDLVALGRKVGVCAGSHE
                     VIRNLMEKVVDLADSEGARVRCMQKPDEVSEDPGPIREVKKNEEALFALTDGFADVLG
                     GTAWLWSRPEFAESVDVLFVDEAGQMALANVLAIAQAAKSVVLLGDPRQLEHPVKGSH
                     PDGTAVSALEHLLDGEPTIPPDRGIFLEETWRLHPTICEVTSELFYA"
     gene            complement(2941..3807)
                     /locus_tag="VFR25_05860"
     CDS             complement(2941..3807)
                     /locus_tag="VFR25_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940606.1"
                     /translation="MPEWLSLYLVLCWLRFVEYVTWRPTGAIVLRGGSHRGCNAAPRD
                     GVAGRAGFVVANPLWPFRGVFVSGTASDGGFDVDDLARRLEEYRQSARSLLRAEGALL
                     VHLFVVLPVVWSIVGITLAWPYLLGSLLVLHVLVLVTFFHAHRRVFGSSRMERWSAAV
                     PFLLAPPMATAAHLALTKGIAREFHPVAAGFHLCDDGSFRDLALPFLRRSEFPVGDPA
                     TEDRSETPADRRGDLRALAEERLGSIDLRPRDRQSEAAHAYCPRCLSEYAEGDRTCVD
                     CAGVALRAFPTS"
     gene            complement(3810..4691)
                     /locus_tag="VFR25_05865"
     CDS             complement(3810..4691)
                     /locus_tag="VFR25_05865"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HET9940607.1"
                     /translation="MFHSFRLPLIALVLAIFALPSTGSAQVDSAGVNLMRSIEWQEGP
                     ATGRLGDLAEVQVPAGMVFTGREGTKKWMEATHNLTSDDDLGIVTPTSENEKWWVIFT
                     NNDVGHVMDDDKDKLDADALMKTLQSNTRESNKERQKRGWSTFEITGWERPPFYDSRT
                     NNLTWAVRLRGSRGESVNYSVRILGREGYMSADLVLRPEELAASIPAFEKLLAGYTYT
                     QGNRYAEYRKGDKLAAYGLTALVAGGAGAVAMKTGLLAKFWKLIVVGVLGLVAAVKRF
                     IKTILGWGKNEETIKSS"
     gene            4847..>5779
                     /locus_tag="VFR25_05870"
     CDS             4847..>5779
                     /locus_tag="VFR25_05870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9940608.1"
                     /translation="MPLTPGTKLGTYEILAPIGAGGMGEVYRARDSRLGREVAIKVLP
                     RDLASTPEIIARFEREARLISSLNHPNICIVHDVGHEGDDFYLVMEMIEGESLADRVG
                     RGPLPTEDVLRFGIQIADALDRAHRAGIIHRDLKPGNVMLARSGAKLLDFGLARSGGG
                     PVAQTTSGINSPTMSRPLTTEGTIIGTFQYMAPEQLEGKEADARTDIWALGCVLYEMA
                     TGKRAFAGKSQASLISAIMSSEPAPIAQVAPMNPPALDRLVRACLAKDPDERIQTAHD
                     VKLQLQWIAEGGSQAGVPAPVAAKRRKREGLAWLW"
BASE COUNT          971 a         2057 c         1845 g          906 t
ORIGIN      
        1 ctcgcataga acagctccga cgtcacctcg cagatcgtgg gatggagccg ccacgtctcc
       61 tcgaggaaga tcccacgatc cggtggaatc gtgggctcgc cgtcgaggag atgctccagc
      121 gcggacaccg cggtgccatc cgggtggctt cccttgaccg gatgctcgag ctgccgcgga
      181 tcgcccagga gcacaacgct cttcgccgcc tgcgcgattg cgagcacgtt ggcgagcgcc
      241 atctgccccg cctcatcgac gaacaaaacg tcgacgctct ccgcgaactc gggacgcgac
      301 cagagccacg ccgtgccgcc aaggacgtcc gcgaacccat ccgtgagcgc aaagagcgcc
      361 tcctcgttct tcttcacctc gcgaatcggg ccgggatctt ccgacacctc gtccggcttc
      421 tgcatgcacc gcacgcgcgc gccttcggaa tccgccagat cgaccacctt ctccattaga
      481 ttccgaatca cctcgtggct tcccgcgcac acgcccacct tccggccgag cgcgacgaga
      541 tcgaggatca ttcgcgcgcc cgtcaccgtc ttgcccgacc caggcggtcc ctggatcccg
      601 agcacggtgg tatccagcga cacgaccaag cgccgcgcag cgtcgatccc gctctcgccg
      661 ggtagccgca agtcgccgcc ggctccaccg tccacccgcg gcggcaacct gagcagcaga
      721 tcccgcgccg cgcggaacgg acccggtgcg gtcaacccgt gatccgcgac ccatccaccg
      781 agccgcatca acgactcggc ctgcttcggc gccgagaaca tcttgtgcga gaagacggcg
      841 cgcggatgct cctccgcgaa cagcatcctc ttcttgatgt cgaccgtgcc cgcggcgaga
      901 tcgatgacct cgacatcgcc gacctcacgc ccatcgggca gcggcgcgtg gagcttgtga
      961 tccgcgcgga tgctcgtctc ctgcttcggg aagcgatagc gatgaatggg cgccttcgcc
     1021 gtcccgccca cgcgctccac gaactcgaga ccgctcaagc ccgcactctc ctccatgagc
     1081 tcctcgtccg acttgcgctg gagatcgaag tactcccacc acggcgcctt ctcctcgcgc
     1141 cggtggaatt cgaggaggtt cgcgagcgtg tagatcgccc gctgctccgg ggtgcgccgg
     1201 tcgggatcgg cgggcatgcc cgtcagcaat gtgttcgtga ggtgccgcgc ccgcgcgtcg
     1261 cgctcgccca gctcctcgct gacttccgtc ttcctcgcga tcggccgcgg gatggcaact
     1321 ccgccgcttt cgatccgcgt ccgctcgtgc tcgagccagt cgcgcagtga catcgcggac
     1381 atgcagtcgt cccggttgta ggcctccacg atcttgcgcg tcggcttgtc gaccgagtcc
     1441 gggctcccca gctcgagcgc gcgctggacc gctcggaggt gcacccccgc ttcgagcagg
     1501 gactgggtgc gcgcgtagcc gaacaccggc tccagtttct tgatcgagta ctcctcgacg
     1561 ctcgcgcgca tggactgccg cacgacggta tggagatcca cgaacaaccc ggcgcgcagc
     1621 atgccatcga tctctgcctc gcgcgtggcg tggcgcccca tcagccgctt gagcgcggcg
     1681 ggctcgtacg ccgcgtagtg gtagacgtgc atctccggat actgctgcca ccgcgccatc
     1741 acctcgtcga tgaagacctc gaacgcgatc cgttccacac tccgatcgag cgcccagagg
     1801 aagtagtgca tgggatcccc ggtctcatcg agagcggccc agccgaagag gtactcctgc
     1861 cctccctgcc ccgcgaacgg atcgccttcg agatcgagga acacatcccc cggggagggc
     1921 ctcgggacga gcgtgaggcc gcgcccctct tcgagcggca gccactcgtg gaccggatcc
     1981 ccggtccgtc gtccttcgag ctgaacgcgc gcttgctcgc gggcacgctc gtacgtctcc
     2041 ttcgcaccgc gcttcggcct ccacgagagc gggatcggct cccccgcgag tccggcgagc
     2101 gtcttgatgc cgcgcgattc cagctcccgc gtctggagct tcgagatgga agcgacgagg
     2161 ctcaggtgat cgtccgcgcg ccgtcggtcg tcgcaggact tccaccaggt gcagatgtcg
     2221 cagtgggcga cgggcaaggg gtaggtgggt gagccggccg catcatcggc gggcgagtcc
     2281 accgcttctt ccgcttgccg ccgcacccgg cggtagtacg cggcgtagtc ggcgacacga
     2341 tagctctcgg gcggaaactc gggccgaggc gggacgatgt gcatgaattc gggcgagacg
     2401 ccctggagcc cagcgaggag atccgagtag agacaaagct gcagcaccga cccgccgcgc
     2461 gtctccaggg cgagcttggt gtcgtagacc tcgtaggacc acgagccaaa gatgctcgac
     2521 cgctccaccc gtcggagcac gtccgcgcgt ccgagccagc gtccctcgac cagcgctgcc
     2581 tggacgatca cgtccacgcc gtccttcatc gccgcgagcg tgcgctcgac ggcttccgtc
     2641 tccggctcga cgtggtcctg cggcgcgcgg gacaaccgga gcccctgcgc ctcgagatgc
     2701 gtcaagtacg cccgctcgtg cttgtccccg agatcgcgaa ggagcgtgag gtccgcacga
     2761 cgcggcggcg gcgcgagtcg tttctccaca acggcgcggt tctgcgccgt gaggtgccgg
     2821 caggcgacgt gattcgcaag atcggtcgcc gagaggcgga tgggggtgcc gtcgatgagc
     2881 ttcatgggag ggggtgagga tgctccgggc gcacccgaag gtcaacgacg gtccggacgg
     2941 tcagctcgtg ggaaaggccc tcagcgccac ccccgcgcaa tcgacgcagg tccggtcacc
     3001 ctcagcgtac tcggagaggc atcgcgggca gtacgcatgg gccgcctccg attgtctatc
     3061 cctgggccga aggtcgatcg accccaaccg ctcctccgcg agggcgcgta gatccccgcg
     3121 gcggtcggcc ggagtttcgg agcgatcctc cgtggccggg tcgcctaccg gaaactcgct
     3181 ccgacggagg aagggcaagg caaggtcgcg gaacgatccg tcgtcacaca ggtggaagcc
     3241 ggccgccacc gggtgaaact ctcgagcgat tcccttcgtg agggccaggt gcgccgccgt
     3301 cgccatgggc ggggccagga ggaacggcac ggccgcggac cagcgctcca tcctcgatga
     3361 cccgaagaca cgacgatgcg catgaaagaa cgtaacgagc acgagaacgt ggaggaccag
     3421 gaggcttccc agcagatacg gccaggccag cgtgatgccg acaatcgacc agaccacggg
     3481 cagcacgacg aagagatgga cgaggagggc gccttcggcc cgaaggaggg atcgcgcgct
     3541 ctgccggtac tcctccagcc gccgcgcgag atcgtccacg tcaaagccac cgtccgaggc
     3601 cgtcccggat acgaagacgc cccggaacgg ccaaagcgga ttggccacca cgaatccggc
     3661 tcggccggcg acaccatcgc gcggcgcagc gttgcagcca cggtgcgagc cgccccggag
     3721 cacgatggcc cccgtgggcc tccacgtcac gtactcgacg aaccggagcc agcagaggac
     3781 gagatagagg gagagccatt ccggcatggt cagctcgact tgatcgtctc ctcgttcttg
     3841 ccccagccga ggatggtctt gatgaagcgc ttcaccgcgg cgacgagccc gagcaccccg
     3901 acgacgatga gcttccagaa cttcgcgaga aggcccgtct tcatcgcgac cgcgccggca
     3961 ccgcccgcaa ccagcgccgt caacccgtac gccgctagct tgtcgccctt ccggtactcc
     4021 gcatagcggt taccctgcgt gtacgtgtac cccgcgagga gcttctcgaa cgcaggaatg
     4081 gaggcggcca gctcctcggg gcgtaggacc agatcggcac tcatgtagcc ttcgcggccc
     4141 aggatccgga cggagtaatt gaccgattcg cccctgctgc cgcggaggcg caccgcccag
     4201 gtcaggttgt tggtgcgcga gtcgtagaag ggtggacgct cccagcccgt gatctcgaag
     4261 gtgctccaac cccgcttctg tcgctccttg ttcgattccc tggtgttgct ctgcagcgtc
     4321 ttcatcagag catcggcgtc gagcttgtcc ttgtcgtcgt ccatgacgtg ccccacgtcg
     4381 ttgttcgtga agatgaccca ccatttttcg ttctccgacg tgggggtcac gatccccagg
     4441 tcgtcgtccg aggtcaggtt gtgggtcgcc tccatccact tcttggtgcc ttcacgcccg
     4501 gtgaacacca tgccggcggg aacttgcacc tctgcgaggt ctcccagccg gccggtcgcg
     4561 ggtccttcct gccactcgat ggaccgcatg aggttcacac cggcagagtc gacctgcgcc
     4621 gagcccgtgg acgggagcgc gaaaatcgcg agaacgagag cgatcagggg gagccgaaac
     4681 gagtggaaca tcggagcctc cacttgaggg gcggagcgct tgtccaggca aggtgcagac
     4741 tgtccctgag gcatcggcac cggggaggca aatctatagc tggagcccct atccgtcgct
     4801 gggggcccta tcccatcgca ccccgaaccg ttacactccc gcccgcatgc cgcttacccc
     4861 cgggaccaag ctaggcacct acgagatcct cgcgcccatc ggcgccggag gcatgggcga
     4921 ggtctatcgc gcacgcgatt cgcggctcgg ccgtgaggtc gcgatcaagg tcctcccgcg
     4981 cgatctcgcc agcacacccg agatcatcgc gcgcttcgag cgcgaggcgc ggctcatctc
     5041 ctcgctcaac caccccaaca tctgcatcgt ccacgacgtc ggtcacgagg gggacgactt
     5101 ctacctcgtg atggagatga tcgaaggcga atcgctcgcc gatcgcgtcg gtcgcggccc
     5161 gctcccgacc gaggacgtgc tccgcttcgg catccagatc gcggacgcgc tcgaccgcgc
     5221 gcacagggcc ggcatcatcc acagggatct caagcccggg aacgtgatgc tcgcgcgctc
     5281 cggcgcaaag ctcctcgatt tcggactcgc gcgaagcggg ggcggcccgg tcgcccaaac
     5341 cacctccggc atcaactccc ccacgatgag ccgtccgctc acgacggaag gcaccatcat
     5401 cggcaccttc cagtacatgg cccccgagca gctcgagggg aaggaagccg acgcccgcac
     5461 ggacatctgg gcgctcggct gcgtcctcta cgagatggcc accggcaagc gcgcattcgc
     5521 cggaaagtcg caggcctctc tcatctccgc gatcatgtcc tcggagcccg cgccgatcgc
     5581 gcaggtggcg cccatgaacc cgcccgcgct cgaccgactc gtgagggcct gcctcgccaa
     5641 ggatccggac gagcgcatcc agaccgcgca cgatgtgaag ctgcagctcc agtggatcgc
     5701 cgagggcgga tcgcaggccg gtgtgccggc gccggtcgcg gccaagcggc gcaagcgcga
     5761 gggcctcgcg tggctgtgg
//
LOCUS       DASTKC010000147         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_116757, whole genome shotgun sequence.
ACCESSION   DASTKC010000147 DASTKC010000000
VERSION     DASTKC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116757"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="VFR25_05875"
     CDS             <1..1678
                     /locus_tag="VFR25_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HET9940609.1"
                     /translation="GPFLARLVPTVVRQMAAYEEYAYLNREESLVADAMREEESRFAE
                     TLEQGMARFEETASQLEKRHETIFPGAVAFQLYDTFGFPLDLTVEMAMERGLTVDQPG
                     YETAMQEQRDRARKAGRFETRRADAGPWQALTQGAHSAFTGYDQWALDGVSIRSLREM
                     GSDRELELTLDRTPFYPEGGGQVGDTGWLQSEGARLRVIDTYRDGDAILHRAEIVEGA
                     AHAGPWRAVVDAEKRAATQRNHTATHLLHAALRARLGTHLKQAGSLVAPDRLRFDFSH
                     GKALTPEDVHAIESAVNERVLADLEVHTEITSLKEAQAKGAMALFGEKYGERVRQVIV
                     SEYSRELCGGCHVRRTGEIGYFRVESEAAIAAGTRRIEAVTGTLAYAHAERDRALLRE
                     LSARVGVPKDQIASRLATLQEETKRLHEERSKAGKAELKTRVAELVQAASSGDPPVVL
                     ALVEASSVEELREASDLVRQGLPRGGGLLAAVIDGKLSVAASVGAEAVERLPADQWVR
                     DAVSVVGGKGGGKRELAVAGAKDASRVREVLDRGRAFAKERLEQPTGGNR"
     gene            1675..2739
                     /locus_tag="VFR25_05880"
     CDS             1675..2739
                     /locus_tag="VFR25_05880"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HET9940610.1"
                     /translation="MKALILSGGKGTRLRPITHTSAKQLVPIANKPILFYGIESIRDA
                     GVTDVGIVVGDTEPEIRAAVGDGSAFGVKVTYIRQHAPLGLAHAVKVSQEFLGQDTFV
                     MYLGDNLILDGITPLVEEFRRDRPNSQILLAKVPHPEEFGVAELNGDRIVKLVEKPKQ
                     PRSDLALVGVYMFDSTIFEAVDGIKPSFRNELEITDAIQWLIDHGKVVHHHVIGGWWK
                     DTGKLEDILEANRMVLSRAKRAIHGTTDSDTRIEGDVVVGKGSVVKNSLLRGPLCLGE
                     RCMIENAYVGPFTSIYDDVKIRNSEIEHSIVLERCTIEDIPVRIEASLIGKESTIQRS
                     RRKPSAYRFMVGDASSVDVL"
     gene            2747..3604
                     /gene="rfbD"
                     /locus_tag="VFR25_05885"
     CDS             2747..3604
                     /gene="rfbD"
                     /locus_tag="VFR25_05885"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET9940611.1"
                     /translation="MRLLVTGANGILGRAIAERLGGAHTLLLWGREEADLTDPAQVEA
                     AARGLTFDAVIHAAAMTNVDLCETERDLAFRSNRDATRHVGALAKERGATLVYVSSDY
                     VFDGTKTSPYMEEDPTGPKSVYGSSKLAGEEAAAASGAPYLVVRTSWLFGAGGRNFVD
                     TIAGKLAQGAALRVVDDQRGSPTYARDLAHAIELLLRRGAQGIVHATNSGQTTWYGFA
                     REIASAMGLADAGITPVSTEEFPRPAPRPRYSVLSGARFRALTGENLRPWEEAVRQYL
                     SARSSAEAK"
     gene            3601..>4021
                     /locus_tag="VFR25_05890"
     CDS             3601..>4021
                     /locus_tag="VFR25_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9940612.1"
                     /translation="MTPRASSVRRGLAEGARLLALTLETEEERIEEMADLFAKAAVQR
                     RTIFFCGNGGSAAEAQHLATELVARFLTDRAAIPAVALTTDTSLLTAVGNDTGFDQVF
                     ARQIEALGRRNDLLVAMTTSGRSSNILAAVAAARRRGM"
BASE COUNT          657 a         1360 c         1449 g          555 t
ORIGIN      
        1 ggggcccttc ctcgcgcggc tcgtgcccac ggtcgtccgg cagatggcgg cgtacgagga
       61 gtacgcgtat ctgaaccgcg aggagtcgct cgtggcggac gcgatgcgcg aggaggagtc
      121 gcgcttcgcg gagacgctcg agcagggcat ggcccgcttc gaggagaccg cgtcgcagct
      181 cgagaagcgg cacgagacga tcttcccggg ggcggtcgcg ttccagctct acgacacctt
      241 cggattcccg ctcgacctca cggtcgagat ggcgatggag cgcgggctca ccgtggatca
      301 gccgggctac gaaaccgcca tgcaggagca gcgggaccgc gcgcggaaag cgggacgctt
      361 cgagacgcgc cgcgcggacg cggggccgtg gcaggccctc acccagggcg cgcactccgc
      421 gttcacgggc tacgatcagt gggcgctcga cggcgtgtcg atccggtcgc ttcgcgagat
      481 gggaagcgac cgcgagctgg agctcacgct cgacaggacg cccttctacc ccgaaggggg
      541 cgggcaggtc ggggacaccg gatggctcca gagcgagggg gcgcgtctcc gcgtgatcga
      601 tacctaccgg gacggcgacg cgatcctgca ccgcgcggag atcgtggagg gcgcggctca
      661 cgccggcccc tggcgcgccg tggtcgacgc ggagaagcgc gcggcgacgc agcggaatca
      721 cacggcgacc catctcctcc atgccgcgct gcgcgcgcgc ctcggcacgc atctgaagca
      781 ggccggctcg ctcgtcgcgc cggatcggct ccgcttcgac ttctcgcacg gcaaggcgct
      841 cacgcccgag gacgtccacg ccatcgaatc cgcggtcaac gagcgcgtgc tcgcggacct
      901 cgaagtgcat accgagatca cgtcgctcaa ggaagcacag gcgaaggggg ccatggcgct
      961 cttcggcgag aagtacggcg agcgcgttcg ccaggtgatc gtgagcgagt acagccgcga
     1021 gctctgcggc ggctgccacg tgcgccgtac gggggagatc ggttacttcc gggtcgagtc
     1081 cgaggcggcg atcgccgcgg gcacgcgccg gatcgaagcg gtgaccggca cgctcgccta
     1141 cgcccacgcg gagcgggacc gtgcgctctt gcgcgagctc agcgcgcggg tcggcgtccc
     1201 caaggaccag atcgcctcgc ggcttgccac gctccaggaa gagacgaagc ggctccacga
     1261 ggagcgcagc aaggccggga aggccgagct caagacgcgc gtcgccgagc tggtccaggc
     1321 ggcgtccagc ggcgatcctc cggtcgtgct cgccctcgtc gaggcgtcga gcgtggagga
     1381 gctccgcgag gcgagcgatc tcgtgcgcca gggccttccg cgcggcggcg ggctcctcgc
     1441 ggcggtgatc gacggcaagc tctcggtcgc ggcgtccgtc ggagccgagg cggtcgagcg
     1501 tttgcccgcg gatcaatggg tgcgggacgc ggtctcggtg gttggtggca aaggaggagg
     1561 caaacgggag ctcgcggtcg caggagccaa ggacgcctcg cgcgtgcgcg aggtcctgga
     1621 ccggggtcgg gctttcgcca aggagcgcct cgagcagccc acgggaggga atcgatgaag
     1681 gcgctcattc tgagcggcgg caagggaacg cggcttcgtc cgatcacgca cacgagcgcg
     1741 aagcagctcg tcccgatcgc gaacaagccg atcctcttct acggcatcga gtcgatccgg
     1801 gacgccggcg tgaccgacgt cgggatcgtc gtcggggaca cggagcccga gatccgcgcc
     1861 gcggtcggag acggttcggc attcggcgtc aaggtgacct acatccgcca gcacgcgccg
     1921 ctcgggctcg cgcacgcggt caaggtctcg caggaattcc tgggtcagga cacattcgtg
     1981 atgtacctgg gcgacaacct gatcctggac ggcatcacgc cgctcgtgga ggagttccgc
     2041 cgggaccggc ccaacagcca gatcctcctc gccaaggtgc cgcaccccga ggagttcggc
     2101 gtggccgagc tgaacggcga ccgcatcgtg aagctggtgg agaagccgaa gcagccgcgc
     2161 tccgacctcg cgctggtggg cgtctacatg ttcgactcca cgatcttcga ggccgtggac
     2221 gggatcaagc cgtcgttccg gaacgagctc gagatcaccg atgccatcca gtggctcatc
     2281 gaccacggga aggtcgtgca ccatcacgtg atcggcggct ggtggaagga cacggggaag
     2341 ctcgaggaca tcctcgaagc caaccgcatg gtcctctcgc gcgcgaagcg cgccattcac
     2401 ggcacgacgg acagcgacac gcgtatcgaa ggggacgtgg tcgtgggcaa gggctcggtc
     2461 gtcaagaact cgctcctccg cggcccgctc tgtctgggcg agcggtgcat gatcgagaac
     2521 gcctacgtcg gccccttcac gtcgatctac gatgacgtga agatccggaa cagcgagatc
     2581 gagcattcca tcgtgctcga gcgatgcacc atcgaggaca ttccggtgcg gatcgaggcg
     2641 agcctgatcg gcaaggagtc cacgatccag cgctcgagga ggaagccctc ggcctatcgc
     2701 ttcatggtcg gggacgcgag ctcggtggac gttctctagc cggggcatgc gcctcctcgt
     2761 cacgggcgcc aacggcatcc tgggccgcgc catcgcggag cggctcggcg gcgcgcatac
     2821 gctcctcctc tggggacgcg aggaggccga cctcaccgat cccgcgcagg tggaggccgc
     2881 cgcacgcggg ctcaccttcg atgcggtgat ccatgccgcg gccatgacca atgtcgacct
     2941 ctgtgagacg gagcgggacc tcgcctttcg ctccaatcgc gacgccacgc ggcatgtggg
     3001 cgcgctcgcc aaggagcgcg gcgccacgct cgtctacgtg agcagcgact acgtcttcga
     3061 cggcacgaag acctccccct acatggaaga ggatcccacc gggccgaaga gcgtgtacgg
     3121 gagctcgaag ctcgcgggag aggaagcggc cgccgcgtcc ggagcgccct acctcgtggt
     3181 gcggacgtcc tggctcttcg gtgccggggg acggaacttc gtcgacacca tcgcggggaa
     3241 gctggcgcag ggagcggcgc tccgcgtggt ggacgaccag cgcggctcgc cgacctacgc
     3301 ccgcgacctc gcgcacgcga tcgagctcct gctgcgacgc ggcgcgcagg gcatcgtcca
     3361 cgccaccaat tcgggccaga ccacgtggta tggcttcgcg cgcgagatcg cgagcgcgat
     3421 ggggctggcg gatgccggca tcacgccggt gagcacggag gagtttcccc gtcccgcccc
     3481 gcgcccgcgc tattcggtcc tctcgggcgc gcgcttccgc gccttgaccg gtgagaacct
     3541 ccgaccctgg gaagaggcgg tgcgccagta cctgagcgcg cgctcatccg cggaggccaa
     3601 gtgacgccgc gcgcgagctc cgtgcgccgc gggctggccg agggagcgag gctcctcgcg
     3661 ctcaccctcg agaccgagga ggagcggatc gaggagatgg ccgacctctt cgcgaaggcc
     3721 gcggtccagc gccggacgat cttcttctgt ggaaacggcg gaagcgcggc cgaagcgcag
     3781 cacctggcga ccgagctggt ggcgcgcttc ctcacggacc gcgccgcgat acccgcggtc
     3841 gcgctgacca ccgacacctc gctcctgacc gcggtcggga acgacaccgg attcgaccag
     3901 gtcttcgcgc ggcagatcga agccctcggc cggcggaatg atctcctcgt cgcgatgacc
     3961 acgagcgggc gctcgtccaa catcctcgcc gccgtggccg ccgcgcgccg gcgcgggatg
     4021 a
//
LOCUS       DASTKC010000148         5376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_116999, whole genome shotgun sequence.
ACCESSION   DASTKC010000148 DASTKC010000000
VERSION     DASTKC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116999"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFR25_05895"
     CDS             complement(<1..311)
                     /locus_tag="VFR25_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940613.1"
                     /translation="MARNDRTIRRSVQTLAALAFLAVPALASAGPGYVNFLLGQKIFD
                     SDHWDPIDKQSAIGAEGVFGKATWPVSLDAYLARASKEKSKAVDDGFGGTVDVTLDAT
                     T"
     gene            416..2605
                     /locus_tag="VFR25_05900"
     CDS             416..2605
                     /locus_tag="VFR25_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940614.1"
                     /translation="MRPTATRSAPRPKNRPGAPTASTSAPPPAIPDVVAWAGIALALV
                     VLALHVRTYFFLTDDSFISFRYARNLVHGHGLVFNPGGERVEGYTNLLWVLLLAVFAA
                     IGLPMEHMASVLSIAATFALFGIVVRFAWVRLPAGQKWIAVIPALFLAGTRSFAVWAT
                     SGLETRLFDFLLVAGILRLVHEVEHVPADGARPRALAPWIFGLAALTRPDGLLVGGCA
                     FLTEFAYRWLRSGRAPLRAALAWWPMALLVCGHFAFRRAYYGDWLPNTFYAKVGARHS
                     LDAGLRYLALFALEYGAILWIPGIVIGVLSRVRVGAPFLPALFLAAIVPHVLYVATIG
                     GDHFEFRPLDFYWPLLFLLLADGVRFWVTSPKRMALATAYAALVLFGLVWFPLRSHRQ
                     FPKEYNPGFPGTYAGLLNDADRFLAPETDPVFRLPILRGVAGAYRDLMREQTAHFGAV
                     RQEEHRLFRETAEDAARRLNDVFARGALPRDVYVAIDCVGVVPYRTDLRTLDRLGLTD
                     AHVAHGPFLRDVVAHGKYATMDYARERGVDVWSADPVSPVLPVGHSRLEREIRKAVGQ
                     GGDYYAADLGSGEFLDALFPQGIEKARERFPNLALRPLQDSTIVASYVAQLLPGFLSD
                     LEQQRVHPIRIQRVGEICLLSNDLRSAAAVYDAASRAYPLEWQFPWMLAICRKLSGDA
                     AGAAEPLQRAGDILVQRGDQAGPARLGGLFAQLKPGMSLHGLAGPSE"
     gene            complement(2663..3496)
                     /locus_tag="VFR25_05905"
     CDS             complement(2663..3496)
                     /locus_tag="VFR25_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940615.1"
                     /translation="MNARRWALTFVAALTLTLALSACQKASSDVSSTMPPEDQTTAMS
                     QTPSPESAPVTETQTTTEQSVTKLEPVVVKPAPKKSTPKTTTTKSTHAVPASENRTVS
                     LPAGSTFDVELATRIHTGESNVGDKIVAKLITPLKGDDGSTIAPAGAVIHGEIAELTR
                     ASKSRAEEDRASVKLAFTSVETVFGEKTLAATVTNVESLKAGSTTKRDALVIAGSAAA
                     GAILGKVAGHDTKDAMIGGLAGAVIGTGVVMAQKGHELEIPAGSKVSLRSDQPITIVA
                     R"
     gene            3648..5147
                     /locus_tag="VFR25_05910"
     CDS             3648..5147
                     /locus_tag="VFR25_05910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9940616.1"
                     /translation="MAVPVLLALFQIALHLTLGLGYGIFRDELYYWDCANHLAWGYVD
                     HPPLSIAVLSVWKTVFGDSILSMRILPALAGGALILLTARLAGRLGGGSFARGLAAAI
                     AFATPSYLGITGYYSMNAFELLFWAALSFIVLDVIEHGRRRDWVLLGLCVGFGTLNKI
                     SILLAAAAAGVVILAATRLRVLRRPGPYLAVGIAALIVAPHVVWQIQNGWPTREFMEN
                     AQRYKMTVQGPLGFLRGVVMEMGPLMAPWLVAGLVGLLVAPALRRGRPLAWMTLLALA
                     VLTFSRSKPYYAVAAFPSAIAGLSVLMESATSARHGWLRALPVAAAIGDFALAAPFAI
                     PMLPVDSFIAYQRALGMRPPSEERRDTGELPQFLADRFGWPELAAQVAGAVRSLPPGD
                     RARCLIVADNYGEAAAINYYGRRDGLPRAVSQHNTYYLWGHGSGTPTAYVIVGDSREG
                     LEQAFTEVREVARTGAPHAMPDETGVPIWLCRGLKLPLDEAWRRGKHYI"
     gene            complement(5161..>5376)
                     /locus_tag="VFR25_05915"
     CDS             complement(5161..>5376)
                     /locus_tag="VFR25_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940617.1"
                     /translation="GVALAGLGRPREAVEGYDEALRIRPELVYRQRARADARDALGDT
                     LLAKEDRERAAAKERDSYAPALDPFRY"
BASE COUNT          704 a         1896 c         1801 g          975 t
ORIGIN      
        1 tacgtcgtcg cgtcgagcgt gacgtccacc gttccgccga agccgtcgtc gacggccttc
       61 gacttctcct tcgacgcccg cgcgaggtag gcgtccaagc tcacgggcca cgtcgccttc
      121 ccgaacaccc cctcggcgcc gatcgcggac tgcttgtcga ttggatccca gtgatccgaa
      181 tcgaagatct tctgcccgag caggaagttg acgtagccgg gtcccgcgga ggcgagggcc
      241 gggacggcga ggaaggcgag ggcggcgagc gtctggacgg atctccgaat cgttcgatcg
      301 ttgcgagcca tgattcctcc acggggacgt gcggcggaca tgcgccccgg aaaggtagga
      361 ccggaccgtg gggggagcaa gcacgaatct ggcgccgtgg taagctctgc tccccgtgcg
      421 accaaccgcc acgagatccg cgccccggcc gaagaatcgg cctggcgcac ccaccgcttc
      481 cacttccgcc cctccgccgg cgattcccga cgtcgtcgct tgggccggca tcgcgctcgc
      541 gctcgtcgtc ctcgccctcc acgtgcgcac ctacttcttc ctcacggacg attcgttcat
      601 ctcgttccgc tacgcgcgca atctcgtgca cggccacggg ctcgtgttca atccgggggg
      661 cgagcgggtc gagggctata ccaacctctt gtgggtgctc ctgctcgcgg tgttcgcggc
      721 catcgggctc cccatggagc acatggcgtc ggttctgagc atcgccgcca cgttcgcgct
      781 cttcggcatc gtcgttcgct tcgcgtgggt ccgcctgccg gccgggcaga agtggatcgc
      841 ggtgatcccc gcgctcttcc tggcggggac gcggagcttc gccgtctggg cgacgagcgg
      901 actcgagacc cggctcttcg atttcctgct cgtggcgggg atcctccgtc tggttcacga
      961 ggtcgagcat gtcccggccg acggcgcccg tccccgcgcg ctcgcgccat ggatcttcgg
     1021 gctcgcagcg ctcacgaggc cggacgggct gctcgtgggg ggatgcgcgt ttctcacgga
     1081 atttgcgtat cgatggctgc gatccggccg cgcgcccttg cgggcggctc tcgcttggtg
     1141 gcccatggcg ctgctcgtgt gcggtcactt cgcgttccgg cgcgcctatt acggagactg
     1201 gctcccgaac acgttctacg cgaaggtcgg cgcgcggcac tcgctggatg cgggtctccg
     1261 ctacctggcg ctcttcgcgc tcgagtacgg cgcgatcctc tggatcccgg ggatcgtgat
     1321 cggtgtcctc tcgcgcgtgc gcgtcggggc gcccttcctg ccggcgctct tcctcgccgc
     1381 gatcgtcccg cacgttctct acgtggccac gatcggaggg gaccacttcg agttccgtcc
     1441 cctcgatttc tactggccgc tcctgttcct gctcctcgcg gacggcgtgc ggttctgggt
     1501 gacgtccccg aaacggatgg cgctcgcgac ggcctatgcc gctctcgtcc tcttcggcct
     1561 cgtctggttc ccgcttcggt ctcaccgcca gtttcccaag gagtacaacc cgggattccc
     1621 gggcacgtac gccgggctcc tcaacgacgc cgaccgattc ctcgcgcccg agaccgaccc
     1681 ggtgttccgc ctgcccatcc tgcgcggcgt agccggcgcc taccgcgatc tcatgcgcga
     1741 gcagaccgcg catttcgggg ccgtacgcca ggaagagcac cgcctcttcc gcgagaccgc
     1801 ggaggacgcc gcgcgacgct tgaacgacgt cttcgcgcgt ggagcgcttc cgcgcgacgt
     1861 gtacgtggcc atcgactgcg tcggcgtggt gccgtatcgc accgatcttc gcacgctgga
     1921 ccggctcggc ctgaccgacg cgcacgtcgc tcacggccca ttcctgcgcg atgtcgtggc
     1981 gcacggcaag tacgcgacga tggactacgc gcgggagcgc ggcgtggacg tgtggtcggc
     2041 ggatccggtc tcaccggtcc ttccggtggg gcacagccgg ctcgagcgtg agatccgaaa
     2101 ggcagtgggg caggggggcg attactacgc cgccgatctc ggctcgggcg agttcctgga
     2161 cgcgctcttc ccgcagggga tcgagaaggc gcgggagcgc ttcccgaatc tcgcgctccg
     2221 gccgcttcag gacagcacga tcgtcgcgag ctacgtggcg cagctcctgc ccggcttcct
     2281 gtccgacctc gagcagcagc gcgtccaccc gattcggatc cagcgggtcg gcgagatctg
     2341 cctcctgtcg aacgaccttc gctcggcggc cgccgtctac gacgcggctt cgcgcgcgta
     2401 tccgctcgag tggcaatttc cctggatgct cgccatctgc cggaagctct ccggcgacgc
     2461 agcgggagct gcggagcccc ttcagcgcgc cggggacatc ctggtccaga ggggggatca
     2521 ggccggcccc gctcgactcg gcgggctctt cgcccagctc aagcccggca tgtcgctgca
     2581 cgggctcgca ggtccgagcg aatgaagaag cgctccccgc ggaacccgcg aggagcgccc
     2641 tgaagcttcg aagccgtgtt gcttagcgtg cgacgatcgt gatcggctga tccgaccgca
     2701 gcgacacctt gctgccggcc ggaatctcga gctcgtgccc cttctgcgcc atcacgacgc
     2761 cggtgccgat gacagcgccc gcgaggccgc cgatcatcgc gtccttcgtg tcgtggccgg
     2821 ccaccttgcc cagaatggct cccgccgccg cgctgcccgc gatcacgagc gcgtcgcgct
     2881 tggtggtgct gcccgccttg agcgattcga cgttcgtgac cgtcgcggcc agcgtcttct
     2941 cgccgaacac cgtctcgacc gacgtgaacg cgagcttcac cgaagcgcgg tcctcttccg
     3001 cccgcgactt gctcgcgcgc gtgagctccg cgatctcgcc gtgaatcacg gcgcccgcgg
     3061 gagcgatcgt cgagccgtca tctcccttca acggcgtgat gagcttcgcc acgatcttgt
     3121 cgcccacgtt gctctcaccc gtgtggatgc gggtggcgag ctcgacgtcg aacgtcgatc
     3181 cggccgggag cgacaccgtg cggttctccg acgcaggcac ggcatgcgtc gacttcgtgg
     3241 tcgtggtctt cggcgtggac ttcttcggcg ccggcttcac gacgaccggc tcgagcttgg
     3301 tgaccgactg ttccgtcgtg gtctgggtct cggtgacggg agcggactcc ggcgacggag
     3361 tctggctcat ggccgtggtt tgatcctcgg gtggcatcgt cgacgagacg tcggacgagg
     3421 ccttctggca ggccgacagg gccagcgtga gtgtgagcgc ggctacgaag gtgagagccc
     3481 agcgacgtgc attcatgaac aacctccccc atgagttcga ccaaaatcgc cgcggtcgac
     3541 ccgcggcgcg cgacccagga gttatcggca caaggggctc atttctgaac cgatttaagc
     3601 ccgggccggc catgcggatg gcgtcgcgat gaggtatcgt gcccgccatg gccgtccccg
     3661 tccttctcgc gctattccag atcgcgctcc acctcacgct gggtttgggc tacggcatct
     3721 tccgggacga gctctactac tgggactgcg cgaaccacct cgcgtggggt tacgtcgatc
     3781 acccgccgct atccatcgcg gtcctgtccg tgtggaagac ggtcttcggc gactcgattc
     3841 tctcgatgcg gatcctgccc gcgctcgccg ggggagcgct catcctcctg acggcgcggc
     3901 tcgccggccg gctcggaggc gggagcttcg cgcggggcct cgccgccgcc atcgcgttcg
     3961 cgacgccgtc ctacctcgga atcaccggct attactccat gaacgcgttc gagcttctct
     4021 tctgggccgc gctctcgttc atcgtcctcg acgtcatcga gcacgggcgg cgccgcgatt
     4081 gggtcctgct cggcctgtgc gtcggattcg gcaccctcaa caagatcagc atccttctcg
     4141 cggctgccgc ggccggcgtc gtgattctcg cggccacgcg gctaagggtg ctccggcggc
     4201 cggggcccta cctggcggtg gggatcgcgg cgctgatcgt ggcgccgcac gtcgtgtggc
     4261 agatccagaa cggatggccc acgcgcgagt tcatggagaa cgcgcagcgc tacaagatga
     4321 cggtccaggg accgctcggc ttcttgcggg gcgtggtgat ggagatgggc cccttgatgg
     4381 cgccatggct cgtggccggt ctcgtcggcc tcctggtcgc gccggcgctt cgacgaggcc
     4441 gtccgctcgc atggatgacg ctcctcgcgc tggccgtgct cacgttcagt cgaagcaagc
     4501 cctactacgc ggtcgctgcg tttccttccg cgatcgctgg actctccgtc ctcatggaga
     4561 gcgcgacttc ggcacggcac gggtggctgc gcgcgcttcc ggtggcggcg gcgatcggtg
     4621 acttcgccct ggccgctcca ttcgcgatcc caatgctgcc cgtcgactcc ttcatcgcct
     4681 accagcgggc gctcgggatg cggcctccgt ccgaggagcg gcgggacacg ggcgagctgc
     4741 cccagttcct cgccgaccga ttcggctggc cggagctggc ggcccaggtg gcgggagccg
     4801 tgcggtccct gcctcccgga gatcgcgcgc gctgtctgat cgtggccgac aactacgggg
     4861 aggccgcggc gatcaactac tacgggcgtc gcgatggctt gccgcgcgcc gtgagccagc
     4921 acaacaccta ttacctatgg ggccatggct cgggcactcc gactgcctac gtcatcgtgg
     4981 gggacagccg ggagggtctc gagcaggcct tcaccgaagt gcgagaagtc gcgcgcacag
     5041 gcgccccgca cgcgatgccg gacgagaccg gggtgccgat ctggctttgc cgtgggctca
     5101 agcttcccct cgacgaggcg tggcgacggg ggaagcacta catctgattc cggtgcgctc
     5161 tcagtaccgg aaggggtcga gcgcgggcgc gtacgagtcg cgctccttcg cggccgcgcg
     5221 ctcccgatcc tccttcgcga ggagcgtgtc accgagggca tccctcgcgt cggcccgggc
     5281 gcgctggcga tagacgagct ccgggcggat gcgaagcgcc tcgtcgtatc cctccaccgc
     5341 ctcgcgcggc cgcccgagtc ccgcgagggc gactcc
//
LOCUS       DASTKC010000149        18909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2009859, whole genome shotgun sequence.
ACCESSION   DASTKC010000149 DASTKC010000000
VERSION     DASTKC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18909
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009859"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /gene="rsfS"
                     /locus_tag="VFR25_05920"
     CDS             complement(<1..267)
                     /gene="rsfS"
                     /locus_tag="VFR25_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9940618.1"
                     /translation="MNEPSPAAAPNHDLAREAARLTLTKRAEDVVILDLRALDGVCDF
                     FVIATGNSEVQVRAIADAVEEGLRKSRGMKPWHSEGFEARRWILL"
     gene            complement(264..1283)
                     /locus_tag="VFR25_05925"
     CDS             complement(264..1283)
                     /locus_tag="VFR25_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9940619.1"
                     /translation="MARAALNRSRVPIEEPTLETIRIPIADAFEQFQARWDDAFLCDV
                     QLVGAVGAHLHRAFGKRFRPTLVLLAAKAFGDVDEDALLGAVVVELIHTATLIHDDSV
                     DKSLLRRGLPTVNNAFNNDVAILMGDYLYSKAFSIMVRARMYDAMDLLAEATHRMSQG
                     ELRQIEQRNRVDLTEVEYMRVIEEKTACLIAAGCELGALAGGGSFEAHSALGRFGGAV
                     GLAFQITDDLFDYLGAESVMGKGTGSDLEGGKITLPLIKSLRNAPERDRRAMVDMIKN
                     REFRNGHWSDVVRFVTEYGGVEYSQARSIELAERAEDELKHVPDRDAREALRDAVHYA
                     VTRHR"
     gene            complement(1334..2707)
                     /locus_tag="VFR25_05930"
     CDS             complement(1334..2707)
                     /locus_tag="VFR25_05930"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9940620.1"
                     /translation="MRLHSLNPKLVPPAFLGCARALRERGHLAYFVGGCVRDLLRGVA
                     VADWDIATSALPEQVLSIFSGAVPTGLKHGTVTVPTPSGNCEITTFRVESGYTDARHP
                     DKVEFVADVSQDLARRDFTVNAIAWDALDKREEDPFGGRSDLTARVIRAVGDPRERFR
                     EDGLRPVRAARFAATLEFDLDPDTEAALGDARDQVARVAPERIREELVKMLAAPKPSR
                     GFEVLRRAGLLPIVLPELAQCVAVPQNRYHAYDVYYHTLHTVDAAPADKPAVRLAALF
                     HDVGKPITHVERNGDATFYNHQFESERLTIEAMTRLRFSREATEEVAHLVRHHMFDYR
                     PEWSDAAVRRFLQKVGTDHIADLFDLRIADNIGNGTKTGFPHYLDELRGRIEQILEAR
                     QALTLSDLAIDGGDVMRELGIPPGRKVGEILERLLDEVLEDPGLNERGRLLRRVREAF
                     SVDRARA"
     gene            2801..3121
                     /locus_tag="VFR25_05935"
     CDS             2801..3121
                     /locus_tag="VFR25_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940621.1"
                     /translation="MSLCAALLVLPTPVRATVFLSSADQGSWTAADKELHAAGSLAIA
                     ASIRVTGRSEAESFTAAVSVGVLKEIYDATIKPSAKKGASWKDLAADVLGAAAGVLLL
                     RAME"
     gene            complement(3250..4653)
                     /locus_tag="VFR25_05940"
     CDS             complement(3250..4653)
                     /locus_tag="VFR25_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940622.1"
                     /translation="MSPALIAYVAVIVSGGAALLAFSIPEFLHLGLLAITAWIVVSLL
                     AESLWIPTVSGKAMESMASTVDISLLILLGFRPAVVVAAIGFTLANVFISRRVWYKAA
                     FNAGQNVLSIGLAGVAYIAMGGHPLDLDGHWTLTAPSMMLPWAVTILVYFLANTLLVS
                     GAVAIESGRSLLKTWREEYLQTHSLLGSAALFFLSPLVVVSYLSIGYFGLIFFFVPLL
                     LIKEAGAKNLALEKAKDELVSSERLAAKGEMAAEVAHELNNYLAAISGRAQLLLMNMG
                     NTEGNERLKENARIIFEQASNMGVLVKGLMDFSHKELRKQPTRLNDVVRRTVEFIVPQ
                     NKYERVAFDLDLAGDLPELNLDPAQIQQVLLNLFSNAADAMHGVEAKERRIVVHTRTR
                     AGEIELVVEDTGPGIPDSAMKRLFEPSFTTKAEGHGFGLSTCYRIVQNHGGRIAAENN
                     SRHGARFIVVLPTQEAA"
     gene            complement(4759..5466)
                     /locus_tag="VFR25_05945"
     CDS             complement(4759..5466)
                     /locus_tag="VFR25_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940623.1"
                     /translation="MRRSAWVVMTAVTVLALGAPVEPRSAAGSDPASDVRAMVRTILD
                     APEVMSDVVLERSDPFGGDPDVERGKLWYLPGRGIRFKSSRTHGHDMVVDHPKEKFYL
                     YSPNENVIYEAPFEKAPARIRKLIEDPDRVLEKNFRAVEQSRIIHGRSRHGYQILSTG
                     LGDSLTSVAIWVAPDPSTRQLHWLSISTPADSLLIELRALTIRKKAEPGHLTLSAPAS
                     ATVQPLDPREMLGRESR"
     gene            complement(5463..6290)
                     /locus_tag="VFR25_05950"
     CDS             complement(5463..6290)
                     /locus_tag="VFR25_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940624.1"
                     /translation="MRIAQVLCALLVASGCAHAPGASGGAEGAAGKAARDRFLTSFAL
                     VEPATRGAGMLSVHAGAYSRAGMNTRWASVRDSVAVVAYVGPVRALDAVLLGESAFVA
                     LRPYGLALTGAVPKGEGLGGRGLRFLTRPWDFSPPWVREAIERAKVEPVDGGGWRLTG
                     QFEDAKMTHDFVLDLNDKSEPLRLRIQHDGDERSLALVRYGPVRRFDIGRRPQWVEWT
                     RGTTRARLDIEEHERAKLAAIWHAPPPDPDWTVLSLDDPRGRELLHRLLGVIDEVTP"
     gene            6312..7064
                     /locus_tag="VFR25_05955"
     CDS             6312..7064
                     /locus_tag="VFR25_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET9940625.1"
                     /translation="METAIVPFWRPTADWFPDSAWFGMATRVGGVSAGPYATLNLSLG
                     VGDDEAAVRENRRRLRAAAQVPDGGPVMLHQVHGRTIVGADDGGRDADGFVIAAGDPW
                     VAVSAADCAPVALVAEDGSAGALVHCGWRGARDGIGPAAVERLGERGIPAARIVAAIG
                     PCLHACCFPIGPEVAAEFDPAFLKPHPTGQPSLDLPGAIAAALAGAGVPTSRIEIAPE
                     CTALETARFFSHRRDRGLTGRHWALLHLAPRP"
     gene            complement(7018..7659)
                     /locus_tag="VFR25_05960"
     CDS             complement(7018..7659)
                     /locus_tag="VFR25_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9940626.1"
                     /translation="MPFQGWDTAVFRAVNLGLERPWLDPVMKAFTDPGRFKAPLLIAA
                     MLLFSLRRARGLLALVVLVATLAVSDQLSAKVLKPIFKRARPSVVVEDAHKLFGVKKS
                     YSFPSVHAVNFSAALPILAAVFPAGAIPYAVVASLVCLSRVYVGDHWPSDVLGGIALG
                     LAIGWLGRKAFLRLEQNAKRWSWYRSFESWRRVEPGVEPTGEARGEGAPNAGP"
     gene            complement(7666..10761)
                     /locus_tag="VFR25_05965"
     CDS             complement(7666..10761)
                     /locus_tag="VFR25_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9940627.1"
                     /translation="MTTRLPLLVTALAVLALLPALVTPASAQFFGQNKVQYKNFRWKV
                     LRTQHFDIHYYQGTEAAVNDAALMAERSYQRLSRVLNHQIRVRVPLVLYASHTDFEQT
                     NITPELIGIGTGGVTEFLKRRVFLPFTGSYAELDHVMTHELVHAFQVDILFGDNQGLI
                     GNPFAAQVPLWFMEGMAEYLSVGTTDPNTSMWLRDASLEGYLIPITTLAYVGDIRVYR
                     FGQNIWQYIADTYGIAKVGEILKRTRRLGSAERALEASTGLTVEVLSRKWTESVRKTY
                     LPEIVDHQNPSQIATKLTDAERDYSNFNVAASVSPSGLQFVYISDRSMYNDVYLASAL
                     DGKPIKKLISGERTPSFETLRFFNTAIAWSPDEKQIAIPAKVGGEDALYVVDVPSGKV
                     KKRYKFGLDAIYSPTWSPDGTKFAFVGLKNGRSILYLVDSNGKNPRQVLSGRYAVRDP
                     VWSPDGSRLAYSTDQGEGTDVNKLIFGDLKIAFLDLASGQITIPPGQQGKNISPQWTP
                     DGNSIVFVSDRTGISNMYRMDIPTGESVRLSDLLTGVSGLVEESPCISLSRDGKRLLF
                     TAFNRGGWDVYSVRDPDKFIHSPPVLDTAPSIATTPLAPIGTHMEASVGAIEGMPEPM
                     SPLDAEDATTDTTTEVETAAPGDSAHTVGAKAAPAPVDSTLSDYVQQVYSEPLADSTT
                     FQQLPYRPSFSRDFFSGGAVVASNVGFAGSSVLQFSDLLGNHQILAILNLYGNLGESD
                     VYFAYTNLSHRTNYGLAIFQYQKDLLLFTSPQRDDLENQIHRGGSVFLSRPFNRFERM
                     EYGITAAMVNQRVFRYDYEGGTIREIADPGDHIYVAPNLAYVHDNVLYGSTGPINGSR
                     SRVSAEYALGGIAYTTAVLDDRHYTSLTRRLTFARRLLGGASFGDDPQFFRFGGAFTY
                     RGKDYGELIGTRAAIANVELRFPLIEALRVGFPLTLSLGGINGVVFLDAASAWNAGTD
                     PRFFSNVGGLHTEGMHLAYGFGARINLGYFILRYDYGREHGIERGPGESHHFLTFGPE
                     F"
     gene            complement(10794..12002)
                     /locus_tag="VFR25_05970"
     CDS             complement(10794..12002)
                     /locus_tag="VFR25_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET9940628.1"
                     /translation="MTRRLYYDDSHLRTFSATVTGVRKADDRIEVSLDATAFYPGGGG
                     QPPDHGRLGARDVLDAREDAGRVWHRIEGALDEGAKVEGEIAWPRRLDHMQQHTGQHV
                     LSRAFVEVAGADTRSFHLGEQVVTIDVDHAGLDDALLRKVEERANQIVWEDRPVRAHN
                     VTMEEALRFPLRKAPQVEGEVRVVEVDEYDWSACGGTHVARTGEIGVIALLGTERYKG
                     GTRVSFACGGRALRRLRETGDLLRRVCLEFTAGEGDLIRAVSRLKEERDSLDRRIKPL
                     VREALEREAAALLADAVEGPGIRVVARHFAGRDPDEVGTLAAMVASAGGVALLVSGEG
                     ASPRAHFCAPPGTISAAALMGDVSRRHGGRGGGRPESAQGTIPADRVEAALRDALEVV
                     TAGTGKGQNA"
     gene            complement(12100..12597)
                     /locus_tag="VFR25_05975"
     CDS             complement(12100..12597)
                     /locus_tag="VFR25_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940629.1"
                     /translation="MARTRQPKLATLDIAEEVAKRVPGYRGYQDVAQRKDDDRRFRSS
                     VAEHLNGEAHRLERIESQQFRDDYSDLLEEVDAGARKLEYLAESLNLPSASKNGFADE
                     TADLVGQIDRQIVDELDHLHRVVHELEKAYLHDERFEMNLAELRGIVERIADQIEQRN
                     VTIAR"
     gene            complement(12811..14109)
                     /locus_tag="VFR25_05980"
     CDS             complement(12811..14109)
                     /locus_tag="VFR25_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940630.1"
                     /translation="MRRAGWLLAILVLFAASGGCETRDRANPLDPYNNETGGLIQGFN
                     ALAGNRQVEIRWSRVKQNGLVGYRLLRWLPGDTPSVLEDHIASNLTGTVDSAVVNEES
                     YVYQVVAHFQSGDTLASPPDTATPGTRQIIVLGADLPGMYGLTPDGRDILFAQQSQDA
                     YEDMELDENRGILWLSIPLAGSVFRLFPRGADAAPEIQVRYASDVSISDQRGYGWVAD
                     PLAGEVHRYGPSLDDVDGTVPVRAGEARVVEAGKHTPFVWIGNDAGQVFRYTTSDPIT
                     STGEWIVQGAVRAIAIDEGKGQAWVVVRPHGETGTDDLYRIDPVDSTVTGVPFRLANV
                     ADVEFDEARHSLWVSEEGPPGLGSGRLDRLDDQGEIQATRSGIEPFGIHVDKQTGNCW
                     VADLKSKRVLEVAPDGFVELRTTRIETPYLVRVIPGAPTP"
     gene            complement(14106..14882)
                     /locus_tag="VFR25_05985"
     CDS             complement(14106..14882)
                     /locus_tag="VFR25_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940631.1"
                     /translation="MPFSQPTAVVEDTVGTAFLYVETRPESLEVYIDGTLAGRSPLGP
                     IEHAARAVRVRVLPPDPRRFGLGRDAMDVTLVKDGSAHLFFDLRPSVTIESEPMGAIA
                     TVTDRAAGDSLLGATPVTVPPGLIEDASVRFAAPAHADTVISGALLVEDESGTAHVEL
                     RRVVWASPGPTPKTPLIKKAWIQWTMVGIGTALSVSAIPLHQKADDYYEQYLASSNAD
                     EIPYLYDQTRKYDRLAGGALVVGQTLLVSGIVMLLLGQRF"
     gene            complement(14971..17778)
                     /locus_tag="VFR25_05990"
     CDS             complement(14971..17778)
                     /locus_tag="VFR25_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940632.1"
                     /translation="MKKSSIVAGLTAVLLFAAATPSSAQYYAFGKNKVQYESFQWLTL
                     KGEHVEVFYYPEEEPIARIALVLAEESYKDLAAKFQHEVPKTVPMIVYSSHHHFEQTN
                     VSPGFLPEGVQGFTEFLKGRVVLPYTGSYAEFHHVIHHEMVHVFQFSIMDDVYKAHRR
                     ASYVVPPLWFSEGLAEYWSTPWDAQGTMVMSDMVLEGRLPTIDDMWKYNGTFTIYKMG
                     QSLCEYIGTTYGDEALRRLYTEIYKEDNWEKLIQKVTGVSARRLSEDWILAQKRRYFP
                     QVKDRSALNLAAQPRAVSEGINLKPVAAPDTSLLGGYRYFFISARDGYTNIYSATYKG
                     AERQVKPMVRGQRNPQFESFHPFQSRIDISKQGELAFVSKWEGRDALFIMDAKSRKIL
                     VRQRIPKLIAISSPAWSPDGKKVAFSGIEASGQSDLYLMERDSEALTRLTNDFYADSD
                     PTWSPDGSQIAFASDRTRYGKDGHQNLFLLDMKTLEIRYLTCGPWMDSAPKWSPTGDR
                     IAFTSDRGGMLEIYTVDPEGHGARMTHLLSSVQDPSWLPDGKSVLFTGFNRSSFGIYQ
                     MSMNTAAPGDSFSLDQALVEGPAPWTWEETSSQSTEAPGDYKPHYGLDLVQGGISASA
                     TENVGQGFQAAFSDLLGNKVYYFQLGNNASTASEIFSRMNFSAWKINREHRWNYAAGV
                     FHTAGDYVDMGGFDYFERRAGGSVVASYPFSRFRRVDLSMSIFYDKKEMFLGFDREGV
                     LATHSISLVEDNSLWFGTGPRDGGRMNVTGSITTNLNTGRNENVTVLADVRKYFRTSQ
                     FTSVALRLQGRASSGADPQRFAMGGSLTMRGYPYRYFEGTRLLMGNLEYRFPLLDRLV
                     LGVPFRGFGLPGLEAAVFADAGTAWESFERFRDPKGSFGFGLRTSLAGYMVLRYDFAK
                     LTDFHTVRPGWEQEFYIGLDY"
     gene            complement(17925..>18909)
                     /locus_tag="VFR25_05995"
     CDS             complement(17925..>18909)
                     /locus_tag="VFR25_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9940633.1"
                     /translation="PKDELARALDRARSLGITSAHDFDRSATWRAAAELARSGPLDFR
                     LLLSVPVASVEAATALGLAAGHGGDQLRVGPVKMFADGTLGSSTALLEEPYAAGGTGV
                     EVMSPAELQNGCAAAARSGLSVAIHAIGDRAVRNALDAIERTLADGGSFPLPPRIEHI
                     QLSRPEDWPRFKALGVLASVQPIHQVSDRPLARRLWGDRTSRSYAWKSLERAGATLIF
                     GSDAPFDRAGPLLAIQAALLRREAGEPAAEAFHPEQGLTLKRALRAHLEDPHLAAGWP
                     LPLGKISAGFGADLVHFSHDLLQTPNGEWARARVRAVWVGGRRVRVRTGDR"
BASE COUNT         3017 a         6386 c         6390 g         3116 t
ORIGIN      
        1 gaggaggatc cagcggcgcg cctcgaaccc ttcggagtgc cacggcttca tgccgcgcga
       61 cttgcggagt ccttcctcca cggcgtccgc gatggcgcgc acctggacct cggagttccc
      121 ggtcgcgatc acgaagaagt cgcacacccc gtcgagcgcg cgcagatcga ggatgaccac
      181 gtcctcggcg cgcttcgtga gcgtgagccg ggccgcttcg cgggcgaggt cgtggttggg
      241 ggcggcggcc ggcgagggtt cgttcaccga tgccgggtca ctgcgtaatg gaccgcgtcc
      301 cggagcgcct cccgggcgtc ccggtcgggc acgtgcttga gctcgtcttc ggcccgctcg
      361 gccagctcga tcgagcgcgc ctggctgtac tcgacgccgc cgtactcggt gacgaagcgg
      421 acgacgtcgg accagtgccc gttccggaac tcgcggttct tgatcatgtc gaccatggcg
      481 cgccgatcgc gctcgggagc gttccgaagc gacttgatga gcggaagcgt gatcttgcct
      541 ccctcgaggt cgctccccgt ccccttgccc atcacgctct ccgcgcccag gtagtcgaag
      601 aggtcgtccg tgatctggaa cgcgaggccc acggcgccgc cgaagcgccc gagcgcgctg
      661 tgcgcctcga acgatccgcc gccggccagg gctccgagct cgcagccggc cgcgatgagg
      721 caggccgtct tctcctcgat gacgcgcatg tactcgacct cggtcaggtc gacgcggttc
      781 cgctgctcga tctgacggag ctctccctgg ctcatgcgat gcgtggcctc ggcgaggaga
      841 tccatcgcgt cgtacatgcg cgcgcggacc atgatcgaga acgccttcga gtagaggtag
      901 tcccccatca agatggcgac gtcgttgttg aacgcgttgt tcacggtcgg cagtccacgc
      961 cggagcaggc tcttgtcgac cgaatcgtcg tggatgagcg tggccgtgtg gatcagctcg
     1021 acgacgacgg cgcccaggag cgcgtcctcg tcgacgtcgc cgaacgcctt ggccgcgagg
     1081 agcacgagcg tggggcggaa tcgcttgccg aacgcgcgat gcaggtgcgc ccccaccgcg
     1141 ccgacaagct gcacgtcgca gaggaacgca tcgtcccagc gtgcctggaa ctgctcgaag
     1201 gcatccgcga tgggaatccg tatggtttcg agggtcggtt cctcgatggg gactcgcgac
     1261 cggttcagcg cggctcgggc caagtggctc ctattgcttg ggggaaggcc aattacggcc
     1321 cgtcagccta gggtcacgcc ctcgccctgt caaccgaaaa ggcctcgcgg acgcgccgga
     1381 gcagccggcc gcgctcgttc aagcctgggt cctcgaggac ctcgtcgagg aggcgttcca
     1441 ggatttcacc caccttccgc cccgggggga tgcccagctc ccgcatgacg tcccccccgt
     1501 cgatcgcgag gtcgctcagc gtgagcgcct gcctcgcctc cagaatctgc tcgatccgcc
     1561 cgcgaagctc atcgaggtag tgcgggaagc cggtcttcgt gccgttcccg atgttgtccg
     1621 caattcgaag atcgaagagg tcggcgatgt ggtcggtgcc caccttctgg aggaaacgcc
     1681 gcacggcggc gtcgctccat tcgggccggt agtcgaacat gtggtgacga acgaggtggg
     1741 cgacctcctc cgtcgcctcc cggctgaacc gaaggcgcgt catcgcctcg atcgtgaggc
     1801 gctcgctctc gaactgatgg ttgtagaacg tggcgtcgcc gtttcgctcc acgtgcgtga
     1861 tcggcttccc cacgtcgtga aagagcgccg cgagacgcac cgccggcttg tcggccggcg
     1921 cggcgtccac ggtgtggagc gtgtggtagt agacgtcgta ggcgtgatag cggttctgcg
     1981 gcaccgccac gcactgcgcg agctccggga gcacgatcgg gaggagcccc gcgcgccgga
     2041 gcacctcgaa gccccgggac ggtttgggag ccgcgagcat cttcacgagc tcctcccgga
     2101 tccgctccgg ggccacgcgc gccacctgat cccgggcgtc ccccagcgcg gcttcggtgt
     2161 caggatcgag gtcgaactcg agcgtggcgg caaagcgcgc ggcccgcact ggacggagcc
     2221 cgtcctcgcg gaagcgctcc cgcggatctc cgaccgcgcg gatcacgcgc gccgtgagat
     2281 ccgagcgccc gccgaacggg tcctcctcgc gcttgtcgag cgcatcccaa gcgatcgcgt
     2341 tcacggtgaa gtcgcgccgc gcgagatcct ggctcacgtc ggctacgaac tcgaccttat
     2401 cgggatggcg tgcgtcggta tagcccgact cgacgcggaa ggtcgtgatc tcgcagttcc
     2461 cggacggagt cgggacggtg acggtcccgt gcttgagccc cgtcggaacc gctcccgaaa
     2521 agatggagag gacctgctcg ggaagcgcgc tcgtcgcgat gtcccaatcc gcgaccgcga
     2581 cgccgcgcag gaggtcgcgc acgcacccgc ccacgaagta ggcgaggtgc ccccgctcac
     2641 gcagcgcgcg cgcgcagccg aggaacgccg ggggaacgag cttgggattg agggaatgta
     2701 ggcgcatctc cctcgatggt atcagtcttg cgcccgcccg gggcaatgct acgcttcgcc
     2761 catggccccc agggtagccc tacggagtct cgcactagcc gtgtcgctct gcgccgccct
     2821 tctggtgctc cccactcccg tccgggccac cgtctttctc tcctccgccg accagggctc
     2881 ctggaccgcc gccgacaagg agctccacgc cgccggctcc ctcgcgatcg ccgcctccat
     2941 ccgcgtcacc ggacgctcgg aggcggaatc cttcacggcc gccgtgagcg tgggcgtgtt
     3001 gaaggagatc tacgacgcga cgatcaagcc ctcggcgaag aagggcgcga gctggaagga
     3061 cctggctgcg gacgtgctcg gcgctgcggc cggcgtgctt ttgctgcgag cgatggagtg
     3121 acgtcgcgtc gggggtggtg agccgtgctc ggggtcctgc acgcggctcg gcgccgaggc
     3181 ggctagcaaa cacgaggccc taaccgccga gctgcgtgcc ggaccccgag cacgcctccc
     3241 gcccccgggc tacgccgctt cctgcgtcgg cagcacgacg atgaaccgcg ctccgtgccg
     3301 cgaattgttc tccgccgcga tccgcccgcc gtggttctgc acgatgcggt agcacgtcga
     3361 tagcccgaag ccgtgtcctt cggccttggt cgtgaacgaa ggctcgaaca gccgcttcat
     3421 cgcggaatcc ggaatgcccg gccccgtgtc ctcgacgacc agctcgatct cgccggcccg
     3481 cgtgcgcgtg tggacgacga tccgccgctc cttcgcctcg acgccgtgca tcgcgtccgc
     3541 ggcgttgctg aagagattga ggagcacctg ctggatctgc gccggatcca ggttcagctc
     3601 gggcaggtcg ccggccaggt cgaggtcgaa cgcgacccgc tcgtacttgt tctgcggcac
     3661 gatgaactcc accgtgcgcc gcacgacgtc gttgagccgg gtgggctgct tccggagctc
     3721 cttgtgggag aagtccatca gccccttcac gagcacgccc atgttgctcg cctgctcgaa
     3781 gatgatccgc gcgttctcct tgagccgctc gttcccctcg gtgttcccca tgttcatgag
     3841 caggagctgc gctcgcccgg agatcgcggc gaggtagttg ttgagctcgt gcgccacctc
     3901 ggccgccatc tctcccttgg ccgcgagacg ctccgaggag acgagctcgt ccttggcctt
     3961 ctcgagcgcc aggttcttcg cccccgcttc cttgatgagg aggagcggca cgaagaagaa
     4021 gatgagcccg aagtatccga tcgagaggta cgacacgacg acgaggggcg agaggaagaa
     4081 gagcgccgcc gagccgagga gcgagtgcgt ctggaggtac tcctcgcgcc aggttttgag
     4141 gagcgaccgc cccgattcga tcgccaccgc tcccgacacg aggagggtgt tcgcgaggaa
     4201 gtagacgagg atcgtcaccg cccagggaag catcatcgag ggcgccgtca gcgtccagtg
     4261 cccgtcgaga tcgagcgggt gcccgcccat ggcgatgtag gccacaccgg ccagtccgat
     4321 cgagagcacg ttctgcccgg cgttgaacgc agccttgtac cagacgcgcc gcgagatgaa
     4381 cacgttggca agcgtgaaac cgatcgcggc cacgacgacg gcggggcgga acccgagcag
     4441 gatgaggagg ctgatgtcca cggtggacgc catgctctcc atggccttcc cgctcaccgt
     4501 gggaatccag aggctctccg cgagcaggct cacgacgatc cacgccgtga tcgcgaggag
     4561 gccgagatgc aggaactcgg gaatggagaa cgcgaggagc gcggcgccgc ccgagacgat
     4621 gacggctaca taggcaatga gggccggaga cacggacgcc ttcaacctcg accgttaaga
     4681 tcgctgggga cgatcgtcac cggtggcgtt gcgaccattg tggccacggc cgcaagctaa
     4741 cagtgtgtca gcgaagcgtc aacgggattc ccggcccagc atctcccggg gatcgagcgg
     4801 ctgcaccgtc gcgctcgccg gcgcgctcag ggtgaggtgg ccgggttcgg cctttttccg
     4861 gatggtgagc gcccggagct cgatcaggag cgaatcggcc ggggtcgaga tcgagagcca
     4921 gtggagctgc cgggtcgagg gatcgggcgc gacccagatc gccaccgacg tcaggctgtc
     4981 gccgagcccc gtcgagagga tctggtaccc gtgccgcgag cgtccatgga tgatcctcga
     5041 ctgctccacg gcgcgaaaat tcttctcgag cacgcggtcc ggatcctcga tcaacttccg
     5101 gatgcgggcg ggcgccttct cgaagggggc ctcgtagatc acgttctcgt tcgggctgta
     5161 gaggtagaac ttctccttgg ggtggtcgac gaccatgtcg tggccgtgcg tccggctcga
     5221 cttgaaccgg atgccccgcc ccgggagata ccagagcttg ccccgctcca cgtccgggtc
     5281 gcctccgaag ggatcgctcc gctcgagcac gacgtcggac atcacctccg gcgcgtcgag
     5341 gatcgtccgc accatcgccc gcacgtcgga cgccggatcc gagccggccg cgctcctcgg
     5401 ctccacgggc gctccgagcg cgagcaccgt cacggccgtc atcaccaccc acgcgcttcg
     5461 tctcatggcg tcacctcgtc gatcacaccg agaagccggt gcaggagctc ccgcccccgc
     5521 gggtcgtcca ggctcaagac cgtccagtcg ggatccgggg gcggagcatg ccagatggct
     5581 gccagcttcg cacgctcatg ctcctcgatg tccagtcgcg ccctcgtggt tccacgcgtc
     5641 cactccaccc attgcgggcg ccgtccgatg tcgaagcgcc gcacgggacc gtaccgcacg
     5701 agggcgaggc tccgctcgtc gccgtcgtgc tggatgcgaa gcctcagggg ctccgacttg
     5761 tcgttcaggt cgaggacgaa atcgtgtgtc atcttcgcat cctcgaactg tccggtgaga
     5821 cgccaccctc ctccatcgac cggttcgacc ttcgcgcgct cgatcgcctc gcggacccag
     5881 ggcgggctga aatcccacgg ccgcgtgagg aaccggagcc cgcggccacc gagcccctcc
     5941 cccttcggca ccgcgcccgt cagagcgagc ccgtagggcc ggagcgcgac gaacgccgat
     6001 tcgccgagca ggaccgcgtc gagcgcgcgc acggggccga cgtacgcgac gaccgccacg
     6061 ctgtcccgaa ccgaggccca gcgcgtattc attcccgcgc ggctgtaggc gcccgcgtgc
     6121 acggagagca tgccggcgcc gcgcgtcgcg ggctccacca gggcgaacga cgtgaggaag
     6181 cgatcgcgcg ccgccttccc ggcagctccc tccgctcctc cggacgcgcc cggcgcatgc
     6241 gcgcatcccg aagcgacgag aagcgcgcaa agaacttgcg cgattcgcac gagcccgcca
     6301 gagtttcgcg catggagacg gcgatcgttc ccttctggcg tcccaccgcg gactggttcc
     6361 ccgattcggc ttggttcggc atggcgaccc gcgtgggcgg cgtgagcgcg ggcccgtatg
     6421 cgacgctcaa tttgagcctc ggcgtgggcg acgacgaggc cgccgtgcgc gagaaccggc
     6481 gtcggcttcg cgccgcggcc caagtgcccg acggcggacc cgtgatgctg caccaggtcc
     6541 acggccggac gatcgtcggc gcggacgacg gcgggcgcga tgcggacggc ttcgtgatcg
     6601 ctgccggcga tccctgggtg gctgtctccg ccgcggattg cgcgccggtc gcactggtcg
     6661 ccgaggacgg ctcggcgggg gcgctcgtcc actgcgggtg gcgcggagcg cgggacggaa
     6721 tcggccccgc cgcggtcgag cgtctcggcg agcgcggcat tcccgccgcg cgcatcgtcg
     6781 cggcgatcgg accatgcctt cacgcctgct gcttcccgat cggtcccgaa gtggccgcgg
     6841 agttcgatcc cgctttcctg aagccccatc cgacggggca gccctcgctc gatctccccg
     6901 gcgcgatcgc ggccgcgctc gcaggcgcgg gcgtccctac gtcgcggatc gagatcgctc
     6961 cggagtgcac cgcgctcgag accgcgcgct tcttctccca ccggcgggac cggggcctca
     7021 cgggccggca ttgggcgctc cttcacctcg cgcctcgccc gtaggctcga ctccaggctc
     7081 cacgcggcgc cagctctcga agctccggta ccacgaccac cgcttcgcgt tctgctcgag
     7141 gcggaggaac gccttccgcc ccaaccatcc gatcgcgagt cccagcgcga tgccgccgag
     7201 cacgtccgac ggccagtggt cccccacgta cacgcgcgac aggcagacga gcgaggcgac
     7261 cacggcatac gggatcgcgc ctgctggaaa gacggccgcg aggatgggga gcgcggccga
     7321 gaagttgacc gcgtgcaccg acgggaagga atagctcttc ttcacgccaa agagcttgtg
     7381 cgcgtcctcc acgaccaccg acggccgcgc gcgcttgaag atcggcttca gcaccttcgc
     7441 gctcagctgg tccgagaccg caagcgtggc cacgaggacc acgagcgcaa gcagcccgcg
     7501 cgcccggcga agggagaaga ggagcatggc cgcgatcagg agaggcgcct tgaagcgccc
     7561 aggatcggtg aacgccttca tcaccggatc aagccacggg cgctccaagc ccaggttcac
     7621 ggcccggaag accgccgtgt cccaaccttg gaacggcatc cggcgctaga actcgggtcc
     7681 gaaggtcagg aagtgatggg actcgcccgg cccgcgctcg atgccgtgct cccgtccgta
     7741 gtcgtagcgg aggatgaagt agccgaggtt gatgcgcgct ccgaacccgt acgcgaggtg
     7801 catgccctcg gtgtggagtc cgcccacgtt cgagaagaac ctgggatcgg tgcctgcgtt
     7861 ccaggccgac gccgcgtcca ggaagaccac gccgttgatg cctcccagcg agagcgtgag
     7921 cgggaagccg accctgagcg cttcgatgag cgggaagcga agctcgacgt tcgcgatggc
     7981 cgcgcgcgtc ccgatcagct cgccgtagtc cttgccgcga tacgtgaacg cgcctccgaa
     8041 ccggaagaac tgcggatcgt cgccgaagct cgctccgccc aggagccgac gcgcgaacgt
     8101 caggcgccgc gtgaggctcg tgtagtggcg gtcgtcgaga accgcggtcg tgtaggcgat
     8161 gccccccagc gcgtactcgg ccgagacgcg ggagcggctc ccgttgatcg gaccggtcga
     8221 gccgtacagg acgttgtcgt gaacgtaggc caggttgggc gccacataga tgtggtcgcc
     8281 gggatcggcg atctcccgga tggttccacc ctcgtagtcg tagcggaaaa cgcgctggtt
     8341 caccatcgcg gccgtgatcc cgtactccat gcgctcgaac cggttgaacg gccggctgag
     8401 gaagaccgag ccgccgcgat ggatctggtt ctccagatcg tcccgctgcg gggacgtgaa
     8461 gaggaggaga tccttctggt actggaagat cgcgagtccg tagttcgtcc ggtgcgagag
     8521 gttcgtgtag gcgaaataga cgtccgactc gcccaggttt ccatagaggt tcaggatcgc
     8581 gaggatctgg tggttcccga gtaggtcgct gaactggagc acgctcgagc cggcgaatcc
     8641 gacgttggac gccacgacgg cgccgccgct gaagaagtcg cggctgaagg agggccggta
     8701 cgggagctgc tggaacgtgg tcgagtccgc gagcggctcg ctgtagacct gctgcacgta
     8761 atccgagagg gtcgaatcca ccggcgccgg agccgccttg gctcccaccg tgtgcgccga
     8821 gtccccgggc gccgccgtct cgacctccgt cgtcgtgtcc gttgtggcgt cctccgcgtc
     8881 gagcgggctc atgggctccg gcatcccttc gatcgcgccc acgctggctt ccatgtgggt
     8941 gccgatcggc gcgagcggcg tcgtcgcgat ggagggggcc gtatcgagaa ccggcgggct
     9001 gtggatgaac ttgtcggggt cgcggacgga atagacgtcc cagccgccgc gattgaacgc
     9061 cgtgaagagg agccgcttcc cgtccctcga gagcgagatg caggggctct cttccacgag
     9121 tcccgagacg cccgtcagga gatcgctcag ccgcacgctc tcccccgtcg ggatatccat
     9181 ccggtacatg ttcgagatgc cggtgcgatc cgagacgaag acgatcgagt tgccgtccgg
     9241 ggtccactga ggcgagatgt tcttcccctg ctgacccggc ggaatcgtga tctgaccgct
     9301 cgcgagatcc aggaacgcga tcttcagatc gccgaagatg agcttgttga cgtccgtccc
     9361 ctcgccctga tcggtcgagt aggcgaggcg ggagccgtcg ggcgaccaga cggggtcgcg
     9421 caccgcatag cgcccgctca agacctgccg cgggttcttg ccgttcgaat ccaccaggta
     9481 gaggatcgag cgtccgttct tcagccccac gaacgcgaac ttggttccgt cgggagacca
     9541 cgtgggcgag tagatggcat cgagcccgaa tttgtaccgc ttcttcacct tcccggaggg
     9601 aacgtcgaca acgtagagcg cgtcctcgcc tcccaccttg gcggggatgg cgatctgctt
     9661 ctcgtccggc gaccaggcga tcgccgtgtt gaagaatcgg agcgtctcga agctcggcgt
     9721 gcgctcgccc gagatcagct tcttgatggg cttcccgtcg agagccgagg cgagatagac
     9781 gtcgttgtac atgctccggt cggagatgta gacgaactgg agtcccgacg gcgagaccga
     9841 cgcggccacg ttgaagttgg agtagtcgcg ctccgcgtcc gtgagcttgg tcgcgatctg
     9901 gctcggattc tggtggtcca cgatctcagg caggtaggtc ttccgaaccg actcggtcca
     9961 ttttctggag agcacctcga cggtgagccc ggtcgacgcc tcgagcgcgc gctcggcgct
    10021 cccgagcctc cgggtgcgct tcaggatctc gcccaccttc gcgatgccgt acgtgtcggc
    10081 gatgtactgc cagatgttct gcccgaagcg gtaaacgcgg atgtcgccga cgtaggcgag
    10141 ggtggtgatc gggatcaggt agccctcgag cgacgcgtcg cggagccaca tggaggtgtt
    10201 ggggtcggtg gtcccgaccg agaggtactc cgccatcccc tccatgaacc agagcgggac
    10261 ctgcgccgcg aacggattgc cgatcagccc ctggttgtcg ccgaagagaa tgtccacctg
    10321 gaacgcgtgc accagctcgt gagtcatcac gtggtcgagc tcggcgtagg agcccgtgaa
    10381 ggggaggaag acccgcctct tcaggaactc cgtgacgcca cccgtcccga tgccgatcag
    10441 ctcgggcgtg atgttggtct gctcgaaatc cgtgtgcgac gcgtagagga cgagggggac
    10501 gcggacgcga atctgatggt tgagcacgcg gctcaggcgc tggtacgagc gctcggccat
    10561 gagcgccgcg tcgttcacgg cggcctcggt gccctggtag tagtggatat cgaagtgctg
    10621 ggtgcggaga accttccagc ggaaattctt gtactggacc ttgttctgcc cgaagaactg
    10681 ggcggaggcc ggagtgacga gcgccggaag gagcgccagg accgcgagcg cggtcacgag
    10741 aagcgggagg cgggtcgtca tgcggcggcc cctcggcttc aggaatcctc ccctcatgcg
    10801 ttctgccctt tccctgttcc ggccgtcacc acctccaggg cgtcccggag ggctgcttcc
    10861 acccggtcgg ccgggatcgt cccctgtgcc gattcgggcc ttccaccgcc tcgccccccg
    10921 tggcggcggc tgacgtcgcc catgagggcg gctgcagata ttgtacccgg cggggcgcaa
    10981 aagtgcgccc ggggactggc cccttcaccc gagacgagga gggcgactcc cccggccgaa
    11041 gccaccatcg cggcgagagt tccgacctcg tcggggtccc ggccggcgaa gtggcgggcc
    11101 acgactcgga tgccgggccc ttccaccgca tcggctagga gcgccgccgc ctcccgctcg
    11161 agagcctccc ggacgagcgg tttgatccgt cgatcgaggg agtcccgctc ttccttgaga
    11221 cgcgacacgg cccggatcag atccccctct ccagccgtga attcgaggca gaccctacga
    11281 aggaggtcgc cggtctcgcg aaggcggcga agcgcccgcc ctccgcaagc gaaggagacg
    11341 cgggtccctc ctttgtaccg ctcggtcccg agcagggcga tcacgccgat ctcgcccgtg
    11401 cgcgcgacgt gggttccgcc gcacgcggac cagtcgtatt cgtccacctc caccacgcgc
    11461 acctcgccct ccacctgagg cgccttccgg agcggaaagc ggagcgcctc ttccatcgtc
    11521 acgttgtgcg cgcgcaccgg gcggtcctcc cacacgatct gattcgcgcg ctcctcgacc
    11581 ttgcgaagga gcgcgtcgtc taagcccgcg tggtccacgt cgatcgtcac gacctgctcc
    11641 cccaagtgga acgagcgtgt gtcggcgccg gcgacctcga cgaaggcccg cgagaggacg
    11701 tgctggccgg tgtgctgctg catgtgatcg agccggcgcg gccacgcgat ctcgccttcg
    11761 accttggcgc cctcgtcgag agcgccctcg atccggtgcc acacccggcc tgcgtcctcc
    11821 ctcgcgtcga gcacatcccg cgcgccgaga cgcccgtggt cgggaggctg tccaccgccg
    11881 ccgggataga acgccgtcgc atccagcgac acctcgatcc ggtcgtccgc cttgcggact
    11941 ccggtcacgg tcgcggagaa cgtgcggagg tgggaatcgt cgtaatagag gcgccgggtc
    12001 acgaagtctg gatcctcgat gtggctcggg ccggcatgcc ggaacgatac aaaacgggct
    12061 cctccggcac aacgaggagc ccgctcgtat gggcccgcgt cagcgggcga tggtcacgtt
    12121 gcgctgctcg atttgatcgg cgatccgctc gacgatcccg cgaagctccg cgaggttcat
    12181 ttcgaaccgc tcgtcgtgca ggtaggcttt ctccagctcg tggaccacgc gatggaggtg
    12241 atccagctcg tccacgatct gacggtcgat ctggcccacg agatccgccg tctcgtccgc
    12301 gaatccgttc ttcgacgccg acggaagatt gagcgactcg gccaggtact ccagcttgcg
    12361 cgcacccgcg tccacttcct cgagaaggtc ggagtagtcg tcgcggaact gctggctctc
    12421 gatgcgctcc aggcggtgcg cttccccgtt caggtgctcg gcgacgctgc tccggaaccg
    12481 gcggtcgtcg tccttgcgct gcgcgacgtc ctgatagccc cggtagcccg gtacgcgctt
    12541 cgccacctcc tccgcgatgt ccagcgtagc cagcttcggc tggcgtgtcc gtgccatctt
    12601 ccatcctcct caccgttgca gagtgcacgc agggaagccg tcccggggca ttggggagtg
    12661 aaaccttggc caatcggtcc gttcgtccag gaccggaggc acatccccta tccggatggg
    12721 acaccggcac atcccatctt ggagcggctt cgatcgcctt cgcgaccggc cgcgccggcg
    12781 agacgaactg caacgggcat gccacggcgt tcatggcgtg ggagcgcccg gaatcacgcg
    12841 gacgagatat ggcgtctcga ttcgggtcgt gcgtagctcc acgaagccgt ccggagcgac
    12901 ctcgagcacg cgcttgctct tgagatcggc gacccagcag ttgcctgtct gcttgtcgac
    12961 gtggatgccg aagggctcga ttcccgaccg cgtcgcttga atctctccct gatcgtccag
    13021 tcgatccaga cgtcccgagc cgagacccgg aggaccctcc tcgctcaccc aaagcgagtg
    13081 ccgggcttcg tcgaactcca cgtcggcgac gttcgcgagc ctgaacggaa cgccggtgac
    13141 ggtcgagtcg acgggatcga tgcgatagag atcgtcggtt ccagtctctc cgtggggacg
    13201 cacgacgacc cacgcctgcc ccttcccttc gtcgatcgcg atggcgcgca cggcgccctg
    13261 cacgatccac tcgccggtgg acgtgatcgg atcactcgtc gtgtatcgaa agacttggcc
    13321 tgcgtcgttc ccgatccaca cgaagggcgt gtgctttccc gcttccacga cgcgcgcttc
    13381 gccggcccgc acgggaacgg tcccatccac gtcgtccaga gacgggccgt agcgatgcac
    13441 ctccccagct agaggatccg cgacccagcc atagcctcgc tggtccgaga tcgacacgtc
    13501 cgatgcgtag cgcacctgaa tctccggcgc cgcgtcggct cccctcggga agaggcggaa
    13561 cacgcttccc gcgagcggga tcgagagcca gaggatgccc cggttctcgt ccagctccat
    13621 gtcctcgtag gcgtcctgcg actgctgagc gaagaggatg tcgcggccgt ccggcgtgag
    13681 cccgtacatg ccgggaagat cggcgcccag cacgatgatc tgccgcgtgc ccggcgtggc
    13741 ggtgtcggga gggctcgcca gcgtgtcgcc gctctggaag tgcgcgacca cctggtagac
    13801 atacgactcc tcgttcacga cggcgctatc caccgtgccg gtgaggttcg aggcaatgtg
    13861 atcctcgagc accgacggcg tgtcgcccgg gagccatcgc aggaggcgat atccgaccag
    13921 cccgttctgc ttcacgcgcg accagcggat ctccacctgg cggttccccg cgagcgcgtt
    13981 gaatccctgg atgaggccac cggtctcgtt gttgtagggg tcgagcggat tcgcccgatc
    14041 ccgcgtctcg catcctccgc tcgcggcgaa gagcacgaga atcgcgagga gccatcccgc
    14101 ccgcctcaaa atctctgccc caggagcagc atcacgatgc cgctcacgag caaggtctgt
    14161 cccacgacga gagccccacc ggccaggcgg tcgtacttcc gggtctggtc gtagaggtac
    14221 gggatctcgt cggcgttcga ggaggcgagg tattgctcgt agtagtcgtc ggctttctgg
    14281 tggagcggaa tcgccgatac cgagagcgcg gtcccgatgc cgaccatcgt ccactggatc
    14341 caggccttct tgatgagcgg cgtcttcggc gtcggcccgg gcgaggccca gaccacgcgg
    14401 cggagctcga cgtgggccgt gccgctctca tcctcgacca gcaacgcgcc ggagatcacc
    14461 gtgtccgcat gggccggagc ggcgaagcgg accgaggcat cctcgatgag tcctggaggc
    14521 accgtgaccg gcgtcgcgcc caggagcgag tcgcccgcgg cgcgatcggt gaccgtggcg
    14581 atcgccccca tcggctcgct ctcgatcgtg acggaggggc gaagatcgaa gaagagatgc
    14641 gccgacccgt ccttcacgag cgtcacgtcc atggcgtccc gtcccaggcc gaagcgccgg
    14701 gggtcgggcg gaaggacgcg cacgcgaacc gcgcgggccg cgtgctcgat cggtcccagg
    14761 ggcgagcggc cggccagggt gccgtcgatg tagacctcga ggctctccgg acgggtctcg
    14821 acgtagagga atgcggtgcc gaccgtgtcc tcgaccacgg cggtcggctg ggagaagggc
    14881 acgtccgacg ctcgcgcggg ccgcgatgcg gccgccggga gcgcgagcgc cagagcgagc
    14941 gccgctacga agcggcgcga cgcgaggccc ctagtaatcc agtccgatgt agaactcctg
    15001 ttcccagccc ggtcgcacgg tgtggaagtc ggtcagcttc gcgaaatcat agcggagcac
    15061 catgtagccg gcgaggctcg tcctgagtcc gaagccgaag cttcccttcg gatcgcggaa
    15121 gcgctcgaac gactcccatg cggtgcccgc gtcggcgaag accgccgctt ccagtccggg
    15181 aagaccgaag ccgcggaacg gaacgccgag cacgagccgg tcgaggaggg ggaaccggta
    15241 ttcgagattg cccatgagaa ggcgcgtgcc ttcgaagtag cgatacgggt agccgcgcat
    15301 cgtgaggctt ccgcccatcg cgaagcgctg cggatcggcg ccgctcgacg cgcgtccctg
    15361 caggcggagc gcgacggacg tgaactggct cgtgcggaag tacttccgga catcggcgag
    15421 cacggtcacg ttctcgttcc ggccggtgtt caggttcgtc gtgatcgatc cggtgacgtt
    15481 catccggccg ccgtccctcg gccccgtgcc gaaccagagg ctgttgtcct cgacgagcga
    15541 gatcgagtgc gtggcgagca cgccctcgcg atcgaagccg aggaacatct ccttcttgtc
    15601 gtagaagatc gacatcgaga ggtcgacgcg gcggaagcgc gagaagggat agctcgccac
    15661 caccgacccg ccggcccgtc gctcgaagta gtcgaacccg cccatgtcga cgtagtcgcc
    15721 cgcggtgtgg aagacccctg cggcgtagtt ccaccggtgc tcgcggttga tcttccaggc
    15781 cgagaagttc atgcgtgaaa agatctccga cgcggtactc gcattgttcc cgagctggaa
    15841 gtagtagacc ttgtttccga ggaggtcgct gaacgccgcc tggaatccct gccccacgtt
    15901 ctccgtcgcg gacgccgaga tgccgccctg cacgaggtcg agtccgtaat gcggcttgta
    15961 gtcgcctgga gcctcggtgg actgcgagct cgtctcttcc caggtccagg gcgccggccc
    16021 ctcgacgagc gcctggtcga gcgagaacga atccccgggg gcggccgtgt tcatcgacat
    16081 ctggtagatg ccgaaggagc tccgattgaa gccggtgaag agcacgctct tcccgtccgg
    16141 aagccacgac ggatcctgca cgctgctcag aaggtgcgtc atgcgcgcgc cgtgcccctc
    16201 gggatcgacc gtgtagatct cgagcatgcc gccgcggtcc gacgtgaagg caatccgatc
    16261 accggtcggc gaccacttgg gcgccgagtc catccagggg ccgcacgtga gatagcggat
    16321 ctcgagcgtc ttcatgtcga ggaggaagag gttctggtgc ccatccttcc cgtagcgcgt
    16381 gcggtccgac gcgaaggcga tctgcgatcc gtccggcgac cacgtggggt cggagtcggc
    16441 gtagaagtcg ttcgtgaggc gcgtcagcgc ctcgctgtcg cgctccatca ggtagaggtc
    16501 ggactgcccc gacgcctcga tgccggagaa ggccaccttc ttcccgtccg gcgaccaggc
    16561 cggcgacgag atggcgatga gcttcgggat gcgctggcgg accaggatct tcctcgactt
    16621 cgcgtccatg atgaagagcg cgtcccggcc ctcccacttc gacacgaacg cgagctcgcc
    16681 ctgcttcgag atgtcgatcc ggctctggaa gggatggaac gattcgaact gggggttccg
    16741 ctggccgcga accatcggct tcacctggcg ctccgcgccc ttgtaggtcg cgctgtagat
    16801 gttcgtgtag ccgtcgcgcg cggagatgaa gaagtagcgg tagccgccta agagcgacgt
    16861 atcgggggcg gcgaccggct tcaggttgat cccctcggac acggcgcgcg gctgcgcggc
    16921 gaggttcagg gcggagcggt ccttcacctg cgggaagtag cggcgcttct gcgccaggat
    16981 ccagtcctcg gagagtcggc gcgccgagac gccggtgacc ttctggatca gcttctccca
    17041 gttgtcctct ttgtagatct cggtgtagag gcggcggagc gcctcgtccc cgtacgtcgt
    17101 cccgatgtac tcgcagagcg actgacccat cttgtagatg gtgaacgtgc cgttgtactt
    17161 ccacatgtcg tcgatggtcg ggagacggcc ctcgagcacc atgtcgctca tcaccatcgt
    17221 gccctgcgcg tcccacggcg tggaccagta ctccgcaagc ccttccgaaa accagagcgg
    17281 cggcaccacg tacgaggcgc ggcggtgcgc cttgtacaca tcgtccatga tcgagaactg
    17341 gaacacgtgc accatctcgt gatggatcac gtgatggaat tcggcgtagc tgcctgtgta
    17401 cggaaggacg acgcgtccct tcaggaactc ggtgaagccc tgcacgcctt cggggaggaa
    17461 tccgggactg acgttcgtct gctcgaagtg atgatgcgag ctgtacacga tcatcggcac
    17521 ggtcttcggg acttcgtgct ggaatttcgc cgcgaggtcc ttgtagctct cttcggccag
    17581 cacgagcgcg atgcgcgcga tcggctcttc ttccggatag tagaagacct ccacgtgctc
    17641 acctttcagc gtgagccact ggaagctctc gtactgaact ttgtttttgc cgaaggcgta
    17701 gtactgggcg ctcgaaggtg tggcggcggc gaacagcagt acagccgtga ggcctgcgac
    17761 gatgctcgac ttcttcatgt tgagcggcct tcggtcgagt ccgcccggcg agacgactta
    17821 ggtggggtta actgaaacga ttatacgccg tcacgacgtg ctgaatcaac ctgcttcatg
    17881 accggaaatc gcgttccgat gcggtttttc agtggtcggg gagctcagcg atctcccgtc
    17941 ctgactcgga cgcgccgacc gcccacccag acggcccgaa cacgtgcgcg cgcccattcc
    18001 ccgttgggag tttgcagcaa gtcgtggctg aagtgaacca aatccgcccc aaaacccgcg
    18061 gaaattttcc caagaggcag gggccagccc gccgcgaggt gcggatcttc gaggtgggcg
    18121 cggagcgcgc gcttcagggt gagaccttgc tcggggtgga aggcctcggc cgccggctcc
    18181 cccgcctccc ggcgcaggag cgcggcctgg atcgcgagca gcggccccgc gcggtcgaac
    18241 ggtgcgtccg atccgaagat gagcgtcgcc ccggctcgct cgaggctctt ccaggcgtac
    18301 gaccgtgagg tgcggtcgcc ccagagtctt cgggcgagcg ggcggtccga gacctgatgg
    18361 atgggctgga ccgacgcgag cacacccaga gccttgaacc ggggccagtc ctcggggcgg
    18421 ctcagctgga tgtgctcgat ccggggcggg agcgggaacg acccgccgtc cgcgagggtg
    18481 cgttcgatcg cgtcgagggc gttccggacc gcacggtcgc cgatcgcgtg gatcgcgacc
    18541 gagaggccgg accgggccgc ggccgcgcag ccgttctgga gctccgccgg agacatgacc
    18601 tcgaccccag tcccgccggc tgcgtagggt tcctcgagga gcgcggtgga cgaccccagc
    18661 gttccatccg cgaacatctt caccgggccg acccggagct gatcgccccc gtggcccgcc
    18721 gcgaggccga gtgcggtcgc ggcctcgacc gaggccaccg gcaccgagag aaggagccgg
    18781 aagtcgaggg ggccggatcg agctagctcc gccgcggccc gccacgtcgc gctccggtcg
    18841 aagtcgtggg cggacgtgat cccgagggaa cgagcccggt cgagcgcccg ggccagctcg
    18901 tccttgggg
//
LOCUS       DASTKC010000150         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_117680, whole genome shotgun sequence.
ACCESSION   DASTKC010000150 DASTKC010000000
VERSION     DASTKC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117680"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..970)
                     /gene="panB"
                     /locus_tag="VFR25_06000"
     CDS             complement(68..970)
                     /gene="panB"
                     /locus_tag="VFR25_06000"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9940634.1"
                     /translation="MSETPAKYGGGGGTGGAGQGQAPPARAKVTVRTIREMKQRGERI
                     TSVTAYDYPSARIADEAGIDLILVGDSLGMVVLGYESTIPVTMAEMSHHLRAVMRAQP
                     KSLVIVDLPFASFQSGPEDAVRNSARLVKRGAEGVKLEGGRRVLAQVDAILAADIPVL
                     GHLGLTPQSVHSFGGYRVQARGREAADALLEDALALERAGAFAIVLEGIPLELGTEVS
                     KALTIPTIGIGAGPGCDGQVLVLHDLIGLSFGKPAKFVRRYADVAGAMRQALTAFKND
                     VRRGSYPAANESYAAAPERDPCAS"
     gene            complement(970..1629)
                     /locus_tag="VFR25_06005"
     CDS             complement(970..1629)
                     /locus_tag="VFR25_06005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET9940635.1"
                     /translation="MTQLLGRYVAIEGVIGVGKTSLARLLAERFRARLVLEEPEANPF
                     LPDFYKDPRRYAFQTQMFFLVSRYKDLRERVHPDLFHEGIVADYLFQKDRIFANLNLS
                     DRELMLYDAIAPQLESEVPAPDLVVYLQASPERILERIQQRGRTYEKLMEPKYTATLA
                     EAYNYFFFHFRDAPLLVVNTDEMNFVDRTNDLEDLITRIVEHKEGVAYVNPGVRAEER
                     G"
     gene            complement(1640..>2055)
                     /gene="folK"
                     /locus_tag="VFR25_06010"
     CDS             complement(1640..>2055)
                     /gene="folK"
                     /locus_tag="VFR25_06010"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET9940636.1"
                     /translation="IPHTSVLRVSSLYDTDPVGDVDQPAYLNAVAWLESELDARTLLW
                     HLLLIEKRMGRVRSQRWGPRSIDLDLLFHGDSQIDEPDLQVPHPEAHRRAFVLHPMLE
                     LDPDFVHPSIGETVKKLVKKLDPTPSVRKGGRFWY"
BASE COUNT          330 a          676 c          728 g          321 t
ORIGIN      
        1 acgaagccga tcgtttcacc gctcctccgg gcgcggagcg aaagctcgcg catggcgtcc
       61 gggcgggtca cgatgcgcaa ggatcgcgct ccggggcggc cgcgtagctc tcgttggctg
      121 cgggatagct accccggcgg acgtcgttct tgaacgccgt gagcgcctgg cgcatcgcgc
      181 cggccacgtc cgcgtagcgt cggacgaact tggccggctt cccgaaggag agcccgatga
      241 gatcgtgcag caccagcacc tggccgtcgc agccggggcc ggcgccgatc cctatcgtcg
      301 ggatcgtgag ggccttggag acctcggtcc ccagctcgag cgggattccc tcgaggacga
      361 tggcgaaggc tcccgcccgc tccagcgcga gcgcgtcctc gaggagcgcg tccgccgctt
      421 cgcgcccgcg cgcctgcacg cggtagcccc cgaagctgtg gaccgactgc ggcgtgaggc
      481 cgaggtgccc gagcacgggg atgtcggcgg ccaggatcgc gtcgacctgc gcgagcacgc
      541 gccggccgcc ttcgagcttc acgccctcgg cgccgcgctt cacgaggcgc gcgctgttcc
      601 gcaccgcgtc ctcggggcct gactggaagg aggcgaacgg gaggtcgacg atcaccagcg
      661 acttcggctg cgcgcgcatg acggcccgga ggtgatgcga catctccgcc atggtcacgg
      721 ggatcgtgga ctcgtagccg agcaccacca tcccgagcga atcgccgacg aggatgagat
      781 cgatcccagc ctcgtccgcg atgcgggcgg acgggtagtc gtaggcggtg acggaggtga
      841 tgcgctcgcc ccgctgcttc atctcgcgga tcgtgcgcac ggtgaccttg gcgcgcgccg
      901 gcggcgcttg gccctggccg gcgccgccgg tgccgccgcc accgccgtat ttcgccgggg
      961 tctccgacat cagccccgct cctctgcccg gacgcccgga ttcacgtagg ccacgccttc
     1021 cttgtgctcc acgatgcgcg tgatcagatc ctcgagatcg ttcgtgcgat ccacgaaatt
     1081 catctcgtcc gtgttcacga cgaggagcgg cgcgtcgcgg aagtggaaga agaagtagtt
     1141 gtaggcctcc gcgagcgtgg ccgtgtactt gggctccatc agcttctcgt acgtgcggcc
     1201 gcgctgctgg atgcgctcga ggatccgctc ggggctcgcc tggagataca ccacgaggtc
     1261 gggcgcgggc acctcggact cgagctgcgg cgcgatggcg tcgtagagca tcaactcgcg
     1321 atcgctcagg ttgagattcg cgaagatgcg atccttctgg aagagatagt ccgccacgat
     1381 cccctcgtgg aagagatccg gatgcacgcg ctcccggagg tccttgtagc ggctcaccag
     1441 gaagaacatc tgcgtctgga acgcgtaccg gcgcggatcc ttgtagaaat ccggcaggaa
     1501 cgggttcgcc tcgggctcct cgagcacgag gcgcgcgcgg aagcgctcgg cgaggagccg
     1561 cgcgagcgac gtcttgccaa cgccgatcac gccctcgatg gcgacgtagc gaccgaggag
     1621 ctgggtcaac ggagcacgct cagtaccaga aacggcctcc cttgcgcacg gacggcgtgg
     1681 gatcgagctt cttgaccagc ttcttcaccg tctcaccgat ggacggatgc acgaagtcgg
     1741 ggtccagctc gagcatcggg tggagcacga acgcgcggcg atgcgcctcc gggtgcggca
     1801 cctggaggtc gggctcgtcg atttgcgaat cgccgtggaa gaggagatcg aggtcgatgg
     1861 agcgcgggcc ccagcgctgc gaccgcacgc gacccatgcg cttctcgatc aagaggagat
     1921 gccagaggag cgtgcgcgca tcgagctcgg actcgagcca cgcgacggca ttgaggtagg
     1981 cgggctgatc gacatcgccg acgggatccg tgtcgtagag cgacgagacg cggagcacgg
     2041 acgtgtgcgg gatgt
//
LOCUS       DASTKC010000151         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2010099, whole genome shotgun sequence.
ACCESSION   DASTKC010000151 DASTKC010000000
VERSION     DASTKC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010099"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFR25_06015"
     CDS             <1..920
                     /locus_tag="VFR25_06015"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HET9940637.1"
                     /translation="SSVLSAVRKTPALISSSSLTMRELTPVDRQFLVERHLISHDLAD
                     NGRLRGLLVVPDESVSAMVNEEDHLRLQALASGFQLRSAWESINTIDDELGQDLDYAF
                     SEDLGYLTACPTNVGTGMRASVLIHLPSLVLTKQIGRVLQGINQVGLAVRGFYGEGSQ
                     IMGNFFQISNQTTLGQSEKETVEGLERVTRQIIEAEQRARDELLKDARVQIEDKIWRA
                     YGTLRHSRVISSQEVVNLSSAVRFGVALAIPGLASVRTLNEVLIRTQPAHVQLAAGKE
                     LEQRERNVLRAELVRALLDANEPDAVNPN"
     gene            961..>3131
                     /locus_tag="VFR25_06020"
     CDS             961..>3131
                     /locus_tag="VFR25_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET9940638.1"
                     /translation="MHDKFTERVRKVMYLAREEAARLQHDYIGTEHLLLGVIREGEGI
                     AATVLNNLGLDLDAIRQAVESMVAATGGTLTIGEIPFTPRAKRVLELSVDEARQLGHN
                     YVGTEHLLLGLIREGEGVAARVLLELGVDRKKVREETLKLLGGTPSVSQPEREGKVET
                     PALNQFGRDLTALAREAKLDPIIGRESEIERVIQVLSRRKKNNPILIGEPGVGKTAIA
                     EGLAERIVENKVPETLKDKRIVTLDLAAVVAGTKYRGQFEERLKAVMNEIRESKDTII
                     FIDELHTIVGAGGAEGAIDASNMLKPALARGELQCIGATTLDEFRKYIEKDGALERRF
                     QPIMVDPPSVDETIQIIMGLRDKYEAHHGAKYTDEAIVSAVKLADRYINDRFLPDKAI
                     DVIDEAGACARLSISAIPTEVRELEKQVEEVAKEKEASIQQQEFEKAARIRDKEKELR
                     AKLRDLKKNWTDVRHETQATVDGDLIGTVIARMTGIPIARIEEKESEKLLRMEEEIRK
                     SIVGQDEAVAAVSRAIRRNRAGLRDPRRPIGSFIFLGPTGVGKTELARVLARFLFDDP
                     DALIRIDMSEYMEKFSVSRLIGAPPGYVGHEEGGQLTEKVRRKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGMLTDSLRRKVDFKNTVLIMTSNLGARQIKGGRSLGFSERDEESAY
                     GGMKRTIMDELKRSFNPEFLNRIDDTIVFHSLSREDITAIVR"
BASE COUNT          599 a         1006 c         1058 g          468 t
ORIGIN      
        1 actcgagcgt gctctccgcg gtccggaaga cgccggctct catatcgtcg tcgtcgctca
       61 ccatgcgcga gctgacgccg gtcgaccgcc agttcctggt cgagcggcat ctcatctcgc
      121 acgacctggc ggacaacgga cggctccgcg gactcctggt cgtgccggac gagagcgtct
      181 ccgcgatggt gaacgaggag gatcatctga ggctccaggc gctcgcctcc ggatttcagc
      241 tccggtcggc gtgggagtcg atcaacacca tcgatgatga actggggcag gacctcgact
      301 atgctttcag cgaagatctc gggtacctga ccgcctgtcc gacgaacgtc ggcacgggca
      361 tgcgcgcttc ggtgctcatc cacctcccgt ccctggtcct gaccaagcag atcggacgcg
      421 tgctgcaagg catcaaccag gtcggactgg ccgtgcgcgg cttctacggc gagggctcgc
      481 agatcatggg caacttcttc cagatctcga accagaccac gctcgggcag agcgagaagg
      541 agacggtcga gggtctcgag cgggtcacgc ggcagatcat cgaagcggag cagcgcgcgc
      601 gcgacgagct cctgaaggac gcgcgggtcc agatcgagga caagatctgg cgcgcttacg
      661 ggacgctccg gcacagccgg gtcatctcgt cccaggaggt cgtgaacctg tccagcgccg
      721 tccgtttcgg cgtggcgctc gcgatccccg ggctggcttc ggtacgcacg ctgaacgagg
      781 tcctcatccg cacacagccg gcgcacgtgc agctcgcggc gggcaaggag ctggagcagc
      841 gggaacggaa cgtccttcgc gcggagctcg tgcgggccct tctggacgcg aacgagccgg
      901 acgcggtcaa tccgaattga gctcgatggc ctaggggaaa tccccggagg ttttcgccaa
      961 atgcacgaca agttcacgga gcgggtgcgg aaggtgatgt acctggcgcg ggaggaggcc
     1021 gcccggctcc agcacgacta catcgggacg gagcacctgc tgctcggtgt gatccgcgag
     1081 ggggagggca ttgccgcgac ggtgctcaac aacctcggcc tcgacctgga cgcgatccgc
     1141 caggccgtgg agagcatggt cgccgccacg ggcggtacgc tgacgatcgg cgagattccg
     1201 ttcacgccgc gggccaagcg cgtgctcgag ctctcggtgg acgaggcccg ccagctcggc
     1261 cacaactacg tgggaaccga gcatttgctt ctcgggttga tccgcgaggg ggaaggcgtc
     1321 gccgcgcggg tcctgctcga gctgggcgtg gaccgcaaga aggtgcgcga ggaaacgctc
     1381 aagctcctgg gagggacccc gtccgtgtcg cagcccgagc gtgaaggaaa agtggagacc
     1441 ccggctctga atcagttcgg ccgggacctg accgcgctcg cgcgcgaggc caagctcgat
     1501 cccatcatcg gacgcgagtc cgagatcgag agggtgatcc aggtgctctc ccgccgcaag
     1561 aagaacaacc ccatcctgat cggtgagccg ggcgtcggga agaccgcgat cgccgaaggg
     1621 ctcgccgagc gcatcgtcga gaacaaggtt cccgaaactc tcaaggacaa gcgcatcgtg
     1681 accctggacc tggcggccgt cgtcgccggg accaagtacc gcggacagtt cgaggagcgc
     1741 ctcaaggcgg tcatgaacga gattcgcgag tccaaggaca cgatcatctt catcgacgag
     1801 ctccacacga tcgtgggcgc cggcggcgcc gagggtgcga tcgacgcgtc caacatgctg
     1861 aagcccgcgc tcgcgcgcgg cgagctccaa tgcatcggcg cgaccacgct cgacgagttc
     1921 cgcaagtaca tcgagaagga cggcgccctc gagcggcggt tccagccgat catggtcgac
     1981 ccgccctcgg tggacgagac gatccagatc atcatggggc tccgcgacaa gtacgaggcg
     2041 catcacggcg ccaagtacac ggacgaggcc atcgtgagcg ccgtcaagct cgcggaccgc
     2101 tacatcaacg accggttcct gcccgacaag gcgatcgacg tcatcgacga ggcgggcgcg
     2161 tgcgcgcgcc tgtcgatctc ggccattccg accgaagtgc gggagctgga gaagcaggtc
     2221 gaagaggtcg cgaaggagaa ggaggcctcg atccagcagc aggagttcga gaaggccgcc
     2281 cggatccgcg acaaggagaa ggagctccgc gcgaagcttc gcgacctgaa gaagaactgg
     2341 accgacgttc gccacgaaac ccaggcgacc gtggacggcg acctcatcgg caccgtgatc
     2401 gcccgcatga cgggtatccc catcgcgcgg atcgaggaga aggaatcgga gaagctcctc
     2461 cgcatggagg aggagatccg gaagtcgatc gtgggccagg acgaggcggt cgccgcggtg
     2521 tcgcgggcca tccgccggaa ccgggccggg ctgcgcgatc cgcgccgccc gatcggctcg
     2581 ttcatcttcc tcggccccac gggcgtcgga aagacggagc tcgcccgcgt gctcgcgcgg
     2641 ttcctcttcg acgatccgga cgcgctcatt cgcatcgaca tgtccgagta catggagaaa
     2701 ttctcggtct cgcgcctgat cggcgcccct ccgggctacg tcggccacga ggaaggcggc
     2761 cagctcacgg agaaggtgcg gcgcaagccc tactcggtcg tgctcctcga cgagatcgag
     2821 aaggcgcacc ccgacgtgtt caacatcctg ctccaagtgc tcgacgacgg catgctgacc
     2881 gacagccttc ggcggaaggt cgatttcaag aacacggtcc tcatcatgac ctcgaacctg
     2941 ggcgcgcgtc agatcaaggg cgggcggagt ctcgggttct cggagcgtga cgaggaatcg
     3001 gcctacggcg gcatgaagcg cacgatcatg gatgagctga aacgctcgtt caatccggag
     3061 ttcctgaacc ggatcgacga caccatcgtc ttccattccc tcagccgtga ggacatcacg
     3121 gcgatcgtcc g
//
LOCUS       DASTKC010000152         8730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_827615, whole genome shotgun sequence.
ACCESSION   DASTKC010000152 DASTKC010000000
VERSION     DASTKC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827615"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..925
                     /locus_tag="VFR25_06025"
     CDS             284..925
                     /locus_tag="VFR25_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET9940639.1"
                     /translation="MKIGILGSGDVGRTLGAGLAARGHDVKLGTRTPEKEAIRDWGTK
                     SGPRTSVGTFADAASFGELVIVATRWDGTENALKLAGPSNLEGKVVMDATNPLTMNGG
                     ELGLALGFSDSGGEQVQRWIPGARVVKAFNTVGTVHMVDPGFSGGPPDMFVAGDDASA
                     KKVVAELASSLGWSVVDLGPLRRARLIEPLAMVWIQHSIDTRSRDHAFKLLRK"
     gene            973..1815
                     /locus_tag="VFR25_06030"
     CDS             973..1815
                     /locus_tag="VFR25_06030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940640.1"
                     /translation="MAPPTIDLPLAMVAGHEAIEETAETDWFRAATPTVVAATGLGVE
                     DLGSVRLYFMPRVDVLMFNRAVGIGIGHPATEDEIDRIVARYRDRGVSRFLVTISPAS
                     KPDSIAPWLEARGFRAHNAWVKLHREAERVPEIATAGLRIEEIGPDRALEFGKVVQAG
                     FSMIDIAGEWVGLTVGRPGWRHYLALDGDKAVGAAAMYLRGPWAWFGFAATLEEARGR
                     GAQSALIARRVTDAHVAGVRWLSIETAQDTPERDAPSFRNVARQGFQVAYLRPNWIWK
                     APGA"
     gene            complement(1861..2553)
                     /locus_tag="VFR25_06035"
     CDS             complement(1861..2553)
                     /locus_tag="VFR25_06035"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HET9940641.1"
                     /translation="MRLHVTRFAVWTVALALALAAPAVRADDKTEDKEAKRLEDARQS
                     FVELTQVKESIPRDLLDKARCVAVFPEVIKGALGWGGRHGKGVMSCRMADGSWGPPIF
                     LTISGGSFGLQIGVEKTELVLFFMNDKAASSLLESKFTLGGKGSVAAGPYGRSAEAET
                     DLKLNAEIYSYAKAKGAFAGISLEGSSLHPDNDALHRFYGSSATPKAVLLEGKAPKHP
                     AAAGRFTEVLPR"
     gene            complement(2640..5504)
                     /locus_tag="VFR25_06040"
     CDS             complement(2640..5504)
                     /locus_tag="VFR25_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9940642.1"
                     /translation="MTLRPPTSVPSTIGPYKIEREIARGGMGIVYLARDMRLDRAVAI
                     KALPDDVAADSERLARFEREARLVASLNHPNIAGIHGIEEAEGRRYLALELVEGESLA
                     DKLARGPLPMSEAIDICLQVASGIEAAHEAGVIHRDLKPGNVLVTASDQVKIVDFGLA
                     KGRVAPESAIGDSPSQTPSGPIPQSPTLNSPTIINSPTIISSPTMAHSPTFGSQTMPG
                     VILGTAGYLSPEQARGKPVDRRTDIWSFGCVLFECLTGKSAFPGETVSDTIAKILERD
                     IDWNALPANTPPAVRDLLHRCLEKDPRKRLRDIGDARLTLEEVKSGVPHTRAPGVDVA
                     PLRPASRHWIFLALVTGLILGIAAGVILGRGKKDEQHVIRLSVPIPHDLRPNQTAMSD
                     DDHYFVMQAVPREAKGDEESTSHLYVRTLEDTEFRLLQGTKGAQIPTISPDGRWVAFI
                     APASERSADNRLMTMPLDGSAPPVPIAKATDDWIGNVLWLQSGGFVISVGQGKKYLRL
                     DKSGVAMGAPKTFTAQGYDGTFVMVSGLPKDRGVLMQGTSYAEGIFHVDIAVLDLKSG
                     NVRILVKDAGSPVYSSTGHLLFTRGDALLAVPFDLGKLQIEGTPVAIEGGLRINQSWM
                     NASFGLTGEGTLRYLLGGDVARDRHAIIVDADGKVSDWSGEHQPFESTFAASADGERF
                     ASIIANDGAIYEIWVSERGRSKSQRVIARAGIDCNNATFSPDGSRLAYSQVSRSASDG
                     IWVVDSNGGGEPRHVGATTPDVQLSPTSWSPDGRWIGGMQSVGGRPEAVILPADGTNA
                     KPVPLFGPGSRTAFPTFSPDGHAIAFVSDQSGRFEIYLVGWDGARVQGQPIIISSGIG
                     SIPVWGSDSKHVYFANPLGNRLMAVAVGTGPRITAGAPTEAWNLEPLRAATAGGGPLL
                     ETMPGNRLVVIQKGANEDDITRYELALHFDEVIREKMRAH"
     gene            5583..7712
                     /locus_tag="VFR25_06045"
     CDS             5583..7712
                     /locus_tag="VFR25_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HET9940643.1"
                     /translation="MRLLISLATSALSLAASAAYAVAWPSPHTLGPYAYVSPAPGSTL
                     QMPGTTIIVRPGGPVDPTSLTPGIIQAVGSASGSHGGFVRLSDDGKTIVFHPDAPFLM
                     GETVTCTIEAGIRSEGALPEESFSFRTFDDSTPGPAPGSSLAMEALDGTSGPSFRAPG
                     FTSSSFDSLPPDFPPIEVTAGAGSEPGYLFLAPLKFSDPAYQSYLLVLANDGTPIFQR
                     KLQSTGLDFKMQPSGRMSYFDTAGSKFYVMNANFAVTDSFEMGYGYYTDVHELQILPD
                     GHALLMAYDGRAVDMSAVVPGGNPNAVVTGLVIQELDRSKDVVFQWRSWDHFQITDAV
                     NRSLTAAQIDYAHGNAIEKDTDGNLLISSRHMSEITKISRDDGHIIWRLGGKNNEFSF
                     VNDPDGFSYQHSIRRLPNGHVILYDNGNFHTPPYSRAVEYDLDESARTATLVWEYRNS
                     PDNYGSAMGSVQRLPNGNTVIGWGATSPALTEVTPTGEKMEEIRFPAGVFSYRGFRHE
                     WPPTIAATAELVRPRIDVGSIGPEISVWVRPDAELFFSADSVDVDSLRIQGTIYATSS
                     GPVDPTRGMEIRFPSAPLLAYLKPGRTQLDIDGTLVSGERFHTSAAIEITGEKRVRAR
                     MASPVGASPVRIAFRSDGATPRTVTMAAYDAHGRRVRHWTATIGGSGEVTWDGRGGNG
                     ERLASGIYFVGTEGTAIDEMARVVLIK"
     gene            complement(7705..>8730)
                     /locus_tag="VFR25_06050"
     CDS             complement(7705..>8730)
                     /locus_tag="VFR25_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940644.1"
                     /translation="GGEDPKLVTQSREMMERQVGQMVHLIDDLLDVSRITQGKLTLRT
                     ERLDLARVVRGAIETSRPIIDERSHTLSVNLPREPVHVDGDPTRLAQVLSNLLNNGAV
                     YTNRGGCLEVRVEHENGWVAIRVRDNGMGMNADTLARVFDLYTQARPTPDTPRIGLGI
                     GLHLSRRLVEMHGGRLEAASEGPGNGSEFVIHIPAAEGIEPPAIERRGGSESLPVFPR
                     RVVVADDNADSAESLALLLRMAGADVRTAHDGEEALEVMAAFHPDVAFIDIGMPSMSG
                     YDVAMKARRSPWGDGLVLIALTGWGQSEDKRLSREAGFDIHLVKPVSASQLHALLSEL
                     GTPTAAT"
BASE COUNT         1393 a         3001 c         2922 g         1414 t
ORIGIN      
        1 gatcgagcgc ggactcgaag tacgcgcgtg cctcgtcgta gtcccccgct tcccacgcgg
       61 cctcgcccgc gacgagcggg tcgctggaag gcacaggagt cgcggcgaac gaatgggacc
      121 aaagcgccgc caggcacgcg agggggaggg cggccaggcg gcgactgcct cgagccataa
      181 ggtctccgat ggtagcaccg cgcgggggcc gggcgtgcaa gccacgaaag cggtacactg
      241 agcgtttggc caaaacggcg ccaagtcgag gagggacagg cagatgaaga tcggaatcct
      301 gggctccggg gacgtgggaa ggaccttggg agcagggctc gcggcacgcg ggcacgacgt
      361 gaagctcggc acccgcacgc ccgagaagga agcgatccgg gactggggga ccaagtcggg
      421 accgcgaacc tcggtcggca cgttcgccga cgcggcttcg ttcggcgagc tcgtcatcgt
      481 cgcgacgcga tgggatggaa ccgagaacgc gttgaagctc gccgggccgt cgaatctcga
      541 gggcaaggtc gtgatggacg cgaccaatcc gctcacgatg aatgggggcg agctcggcct
      601 cgcgctggga ttctcggact cgggtggtga gcaagtgcag cggtggatcc ccggcgcgcg
      661 cgtcgtgaag gcgttcaata cggtcggcac cgttcacatg gtggacccgg gcttctccgg
      721 cggaccgccc gacatgttcg tcgccggcga cgacgcatcc gcgaagaagg tggtcgcgga
      781 gctcgcgtcg tcactgggat ggtccgtcgt ggatctggga ccgctccgcc gcgcgcggct
      841 gatcgagcct ctcgcgatgg tctggatcca gcactcgatc gatacgagat cccgcgacca
      901 cgcgttcaag ctgctccgca agtgaccgcc ccggagcgta cggagggcgt cgggcccacc
      961 gaacctaagc gggtggctcc gcccacgatc gaccttcccc tcgcgatggt ggccgggcac
     1021 gaagcgatcg aggagaccgc ggagaccgat tggtttcgcg ccgcgacccc aacggtcgtg
     1081 gcggcgacgg ggctcggcgt ggaggacctg ggttcggtcc gcctctactt catgccgcgg
     1141 gtggacgtgc tcatgttcaa ccgcgcggtc gggatcggaa tcgggcaccc cgcgaccgag
     1201 gacgagatcg acaggatcgt ggcgcgctat cgcgatcggg gcgtgtcgcg cttcctcgtc
     1261 accatctcgc ccgcctcgaa gccggactcg atcgcgccct ggctcgaggc gcgcggattc
     1321 cgagcgcaca acgcgtgggt gaagctccac cgcgaagccg agcgcgtgcc cgagatcgca
     1381 accgcgggac tccgcatcga ggagatcggc cccgatcgcg cgctcgagtt cgggaaggtg
     1441 gtccaggccg gcttctcgat gatcgacatt gcgggagagt gggtggggct caccgtcgga
     1501 cgtccaggct ggcgccacta tctcgcgctc gacggcgaca aggcggtcgg cgccgccgcg
     1561 atgtacctcc gcggcccctg ggcttggttt ggattcgccg cgacgctcga ggaggcacgc
     1621 gggcgcgggg cccagagcgc actcatcgcg cgccgcgtca ccgacgcgca cgtggccggc
     1681 gtgcgctggc tcagcatcga aaccgcccag gacacgccgg agcgcgacgc tccgtccttc
     1741 cggaacgtag cgcggcaggg attccaggtc gcctacctga ggccgaactg gatctggaag
     1801 gcgcccggag cgtgaggggc gcccgaccgc gcgctctcgg cgcggccgga cgcaacaccg
     1861 tcagcgaggc aggacttcgg tgaagcgccc cgccgccgct ggatgcttcg gcgccttccc
     1921 ctcgagcagg accgccttcg gggtcgcgga cgatccgtaa aatcggtgga gcgcgtcgtt
     1981 gtccgggtga aggctcgatc cttcgagtga gatcccggcg aacgcgccct tcgccttcgc
     2041 gtacgaatag atctccgcgt tcagcttgag atcggtctcc gcttccgcgg accggccgta
     2101 cggcccggcc gcgacgctcc ccttcccgcc gagcgtgaac ttgctctcga ggagcgagct
     2161 cgccgccttg tcgttcatga agaaaaggac cagctcggtc ttctcgacgc cgatctgaag
     2221 cccgaagctt ccccccgaga tcgtgaggaa gatcggcggg ccccacgagc cgtccgccat
     2281 gcggcagctc atcacgcctt ttccgtgccg tcctccccag cccagcgcgc ccttgatcac
     2341 ctcgggaaac acggcgacgc accgggcctt gtcgaggagg tcccggggaa tgctctcctt
     2401 cacctgggtc agctcgacga agctctgccg cgcgtcctcg agccgcttcg cttccttgtc
     2461 ctcggtcttg tcatcggcgc gcaccgctgg agccgcgagg gccagcgcga gcgcgacggt
     2521 ccaaaccgca aatcgggtca catgcagtcg catggttcat cctccgtttc gatcatcccc
     2581 acccatctac catgctacag gacctggaag gatgctgcag tgcagcggag ctcgaggggt
     2641 cagtgcgcgc gcatcttctc ccggatcacc tcgtcgaagt gcagcgcgag ctcgtagcgg
     2701 gtgatgtcgt cttcgttggc gcccttctgg atcacgacga gtcgattccc gggcatcgtc
     2761 tcgaggagcg ggccgccccc ggcggtggcg gcgcgaaggg gttcgaggtt ccatgcttcg
     2821 gtgggcgcgc ccgcggtgat gcggggaccg gtcccgacgg ccacggccat caatcggttc
     2881 cccagaggat tcgcgaagta gacgtgcttg ctgtcggacc cccaaacggg aatgctgccg
     2941 atgcccgacg agatgatgat gggctgtccc tggactctgg ccccgtccca tccgaccagg
     3001 tagatctcga atctcccgct ctggtccgaa acgaaggcga tcgcgtgtcc gtccggcgag
     3061 aaggtcggaa acgccgttcg cgagcccggc ccgaagagcg gcaccggctt cgcgttcgtt
     3121 ccgtccgccg gaaggatcac agcctcgggt cgtcccccca cgctttgcat gccgccgatc
     3181 catcgtccat ccggagacca cgaggtcggg ctcagctgca cgtcgggcgt ggtcgctccg
     3241 acgtggcgcg gctcaccccc tccgttcgaa tccaccaccc agatcccgtc cgacgcggag
     3301 cgggagacct gcgaatacgc aagcctggaa ccgtccggtg aaaaggtcgc gttgttgcaa
     3361 tcgatcccgg ccctcgcgat gacgcgctgc gacttggacc gtccgcgctc cgacacccag
     3421 atctcgtaga tggcgccgtc gttcgcgatg atggacgcga agcgctcccc gtccgccgag
     3481 gcggcgaacg tgctctcgaa cggctggtgc tcgccggacc agtcgctcac cttgccgtcc
     3541 gcgtcgacga tgatggcgtg ccggtcccgt gcgacgtcgc cgcccaagag gtagcgaagc
     3601 gtgccctcgc cggtcaggcc gaaggaggcg ttcatccagc tctggttgat ccggagtccg
     3661 ccttcgatcg cgacgggtgt tccctcgatc tggagcttgc cgaggtcgaa cggcaccgcg
     3721 aggagcgcgt cgccccgggt gaacaggaga tggccggtcg aggagtagac ggggcttccc
     3781 gcgtccttca cgaggatgcg gacgttgccc gacttgagat cgagcaccgc gatgtcgacg
     3841 tggaagatgc cctcggcgta ggacgtgccc tgcatcagga ctccgcggtc cttggggagc
     3901 cccgaaacca tcacgaacgt gccgtcatag ccctgcgccg tgaaggtctt cggcgccccc
     3961 atcgcgacgc cgctcttgtc cagcctcagg tacttctttc cctggccgac gctgatgacg
     4021 aagccgcccg attggagcca gagcacgttt ccgatccagt cgtccgtcgc cttcgcgatc
     4081 ggaaccggcg gcgcgctgcc gtccaggggc atcgtcatca gccggttatc cgccgagcgc
     4141 tccgaggcgg gggcgatgaa cgcgacccag cggccatcgg ggctgatcgt cgggatctga
     4201 gcccccttcg tcccctggag caggcgaaac tccgtgtcct cgagcgtccg gacgtagaga
     4261 tgggacgtcg actcctcgtc ccctttcgcc tcccgcggca cggcctgcat cacgaagtag
     4321 tggtcgtcgt cgctcatcgc cgtctgattg gggcgcaagt catgcggaat cggcaccgac
     4381 aggcggatca cgtgctgctc gtccttcttc ccgcggccga ggatcactcc cgcggcgatc
     4441 ccgaggatca gccccgtcac gagcgcgagg aagatccagt gccgcgaggc cggtcgaagc
     4501 ggcgccacgt cgacgcccgg ggcgcgcgtg tgcggcacgc ccgacttcac ttcctcgagc
     4561 gtgagccgcg cgtcgccgat gtcgcggagg cgctttcgcg gatccttttc gaggcagcgg
     4621 tggaggagat cgcgcacggc cggcggagtg ttcgccggca gcgcgttcca gtcgatgtcc
     4681 cgctccaata tctttgcgat cgtgtccgac acggtctcgc ccgggaaggc ggacttcccc
     4741 gtcaggcact cgaagagcac gcagccgaag gaccagatgt ccgtgcggcg gtcgacgggc
     4801 ttcccgcgcg cctgctcggg ggagagatag ccggccgtgc ccaggatcac accgggcatc
     4861 gtctgagagc cgaacgtggg cgagtgcgcc atcgtcggcg agctgatgat ggtcggcgag
     4921 ttgatgatcg tcggcgagtt gagcgtcggg gattggggga tcggccccga gggcgtctgc
     4981 gacggcgagt caccgatcgc ggactccggc gccacgcgtc ccttcgccag gccgaagtcg
     5041 acgatcttca cctggtcgga cgccgtgacg agcacgttgc ccggcttgag atcgcgatgg
     5101 atgacgcccg cctcgtgcgc ggcctcgatc ccgctcgcga cctgcaggca gatgtcgatc
     5161 gcttcggaca tcgggagcgg cccgcgcgcg agcttgtccg cgaggctctc cccctcgacc
     5221 agctcgaggg cgagataccg gcgtccctcc gcctcctcga tcccgtggat gcccgcgatg
     5281 ttcgggtgat tgagcgacgc gacgagccgg gcctcgcgct cgaagcgcgc gagccgctcg
     5341 gagtcggcgg cgacgtcgtc gggcagcgcc ttgatcgcga cggctcggtc gagccgcatg
     5401 tcgcgcgcga gatagacgat ccccatcccg ccgcgcgcga tctcgcgctc gatcttgtaa
     5461 ggcccgatcg tgctgggcac actggtgggc gggcgaaggg tcaagcgccg atgatagccc
     5521 cggccccggg ccgtatgcta gtctccgtcc cgtttcgtcc aagccgaaat cgaggaacga
     5581 tcgtgcgtct tctcatctcg ctcgccacga gcgcactctc gctcgcggcg tccgccgctt
     5641 atgccgtcgc gtggccgtcc ccgcacacgc tcggccccta tgcctacgtc tcacccgccc
     5701 ccggctcgac cctccagatg cccggcacca cgatcatcgt gcgtccgggc ggaccggtgg
     5761 atcccacgtc cctcacgccc gggatcatcc aggcggtggg atccgcgtcc ggctcgcatg
     5821 gcggattcgt ccgtctctcc gacgatggga agacgatcgt cttccacccc gacgcgccgt
     5881 tcctgatggg agagaccgtc acgtgtacga tcgaggccgg aatccgcagc gagggcgcgc
     5941 tgccggaaga atcgttctcc ttccgaacct tcgacgactc cacgcccggt cccgcgccgg
     6001 gatcgtcgct cgcgatggag gctctggatg gaacctcggg accgtcgttc agggctccgg
     6061 gcttcacgtc gtcgtccttc gattcgctcc cgcccgactt cccgccgatc gaagtgaccg
     6121 cgggcgccgg gtccgaaccg gggtatctat tcctcgcccc actcaagttc agcgatccgg
     6181 cgtaccagtc ctacctcctg gtcctcgcga atgacgggac acccatcttc cagcgcaagc
     6241 tccagagcac cggcctcgac ttcaagatgc agccgtccgg ccgcatgtcc tacttcgaca
     6301 cggccggcag caagttctac gtgatgaacg ccaacttcgc ggtgacggac agcttcgaga
     6361 tgggctacgg ctactacacg gacgttcacg agctccagat cctcccggac ggacacgcgc
     6421 tgctcatggc ctatgacggc cgcgcggtcg acatgagcgc ggtcgtgccg ggcggaaacc
     6481 ccaacgccgt ggtgaccgga ctcgtcatcc aggagctcga ccgctccaag gacgtcgtct
     6541 tccagtggag atcgtgggac cacttccaga tcaccgacgc ggtgaaccgg tctctcacgg
     6601 cggcccagat cgactacgcc cacgggaacg ccatcgagaa ggacacggac ggcaaccttc
     6661 tgatctcgag ccgccacatg tccgagatca ccaagatcag ccgcgacgac gggcacatca
     6721 tctggaggct gggggggaag aacaacgagt tctcgttcgt gaacgatccc gacggattct
     6781 cctaccagca ctcgatccga aggctcccga acggccatgt catcctgtac gacaacggca
     6841 acttccacac gcccccctac tcgcgcgccg tggaatacga tctcgacgag tccgcgagga
     6901 cggccacgct cgtctgggag taccgaaaca gccccgacaa ctacggctcg gcgatgggat
     6961 ccgtgcagcg attgcccaac gggaacacgg tcatcggatg gggcgcgacg agcccggcgc
     7021 tcacggaagt gacgccaacc ggcgagaaga tggaggagat ccgcttcccc gccggcgtgt
     7081 tctcgtaccg tggatttagg cacgagtggc cgcccacgat cgcggcgacc gctgagctcg
     7141 tccgtccgcg catcgacgtc ggatcgattg gccccgagat ctcggtttgg gtgaggcccg
     7201 atgccgagct gttcttctcc gccgattccg tcgacgtcga ttcgctccgg atccagggga
     7261 cgatctacgc cacgtcgtcg gggcccgtcg atcccacgcg cggcatggag atacgatttc
     7321 cgagcgctcc cctcctcgcc tacctgaagc ctggccggac ccagctcgac atcgacggga
     7381 cgctcgtgag cggcgagcgc ttccacacga gcgccgcgat cgagatcacg ggcgagaagc
     7441 gggtgcgcgc gcgcatggcg tctccggtcg gcgcctctcc cgtgcggatt gcgttccgga
     7501 gcgacggcgc cacgccgcgg acggtgacga tggcggcgta cgacgcccac ggcaggcggg
     7561 tgcggcactg gaccgcgacg atcggaggat cgggcgaggt cacctgggat gggcgcggcg
     7621 ggaacggcga gcggctggct tcggggatct acttcgtcgg aaccgaggga accgccatcg
     7681 acgagatggc gcgggtcgtt ctgatcaagt agcggcggtc ggggttccca gctccgacag
     7741 gagcgcgtgg agctggctcg cggataccgg cttcaccagg tggatgtcga acccggcctc
     7801 gcgggacagg cgcttgtcct cgctctgccc ccaccccgtg agcgcgatca gcacgagacc
     7861 gtcgccccac ggcgagcggc gcgccttcat cgccacgtcg tatccgctca tgctcggcat
     7921 cccgatgtcg atgaacgcca cgtcgggatg gaacgcggcc atcacctcga gcgcctcctc
     7981 gccgtcgtgc gccgtccgca catccgctcc ggccatccgc agaaggagag cgaggctctc
     8041 ggcggagtcc gcattgtcgt ccgccaccac gacgcgccgg ggaaacaccg gaaggctttc
     8101 ggaaccgccc ctccgctcga tcgccggcgg ctcgatcccc tccgcggcgg gaatgtggat
     8161 cacgaactcg ctcccgttac cgggaccctc gctcgccgcc tcgaggcgtc ccccatgcat
     8221 ctcgaccaga cgacgcgaga ggtggaggcc gatgccgagg ccgatgcggg gcgtatcggg
     8281 cgtcggtcgc gcctgcgtgt agagatcgaa cacgcgggcg agggtatcgg cattcatgcc
     8341 catgccgttg tcgcgcaccc ggatcgccac ccatccattc tcgtgctcca ctcgaacctc
     8401 gaggcagccg ccgcgattcg tgtacaccgc gccgttgttg aggagattcg agaggacctg
     8461 cgcgagccgc gtgggatcgc catcgacgtg cacgggctcg cgcggcaaat tcaccgagag
     8521 cgtgtgcgag cgctcgtcga tgatggggcg gctcgtttcg atcgcccccc gcacgacgcg
     8581 cgcgaggtcg aggcgctcgg tgcgaagcgt gagctttccc tgcgtgatgc gcgacacgtc
     8641 gaggagatcg tcgatgagat ggaccatctg cccgacctgg cgctccatca tctcgcgcga
     8701 ctgggtcacg agctttggat cttcgccccc
//
LOCUS       DASTKC010000153         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1773395, whole genome shotgun sequence.
ACCESSION   DASTKC010000153 DASTKC010000000
VERSION     DASTKC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773395"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="VFR25_06055"
     CDS             <1..1658
                     /locus_tag="VFR25_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET9940645.1"
                     /translation="EEIIEPLGDRVLGRVAIEDIVDPNTGDVIVRSGEEINEAAAEAI
                     EESGRIEKVSIRSVLKCEAPRGACAKCYGRNLATGRMVDLGEAVGVIGAQSIGEPGTQ
                     LTLRTFHIGGVAGRIVEQSKIKAKTPGKVVFRDLETVERSSGQLVVVGHKGQMEIQGP
                     DGKARQRYSPPYGATLHVRDEIEVNPGDELFEWDTFSTPILTEKPGVVRFVDIKEKVT
                     VRDEVDENTGLRLMVIIDDREKVLHPHVDIIGKGDRRVASYPLPTGARLLVRDGHEVT
                     AGEALVKIRREISKTRDITGGLPRVSELFEARKPKDAATVSEIDGRVEFGGVTRGMKK
                     LIVVGDSGESREYLIPQGKHMHVQEGDYVHAGDRLTEGPVNPHDILKIKGIQAVQEYL
                     VNEIQEVYRLQGVRIDDKHIEVIVRQMLQKVRIEDPGDTNFLEGEQVDKTLLKVEQAR
                     VEAEGGQPATYQPLLLGITKASLSTQSFISSASFQETTRVLTEASVLGMTDHLRGLKE
                     NVIIGHLIPAGTGLSRYRGIDVISEDEEETEKAAPVEEEVKTA"
     gene            1729..2106
                     /gene="rpsL"
                     /locus_tag="VFR25_06060"
     CDS             1729..2106
                     /gene="rpsL"
                     /locus_tag="VFR25_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET9940646.1"
                     /translation="MPTLNQLVRHGRKRQLSKTASPALKAAPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTCYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTL
                     DASGVDGRQQSRSKYGTKRKKGA"
     gene            2110..2580
                     /gene="rpsG"
                     /locus_tag="VFR25_06065"
     CDS             2110..2580
                     /gene="rpsG"
                     /locus_tag="VFR25_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET9940647.1"
                     /translation="MRRAAKIHRVELAPDARYANVLVTRFINCLMLGGKKSTAERIFY
                     GAMDVIEKKTGQDGLSVLKTAMNNVKPVLEVKSRRVGGATYQVPVEVRQDRRTALALR
                     WLIQYSRSRPDHTMADRLAAEITAASKNEGGAVKKREDTHKMAEANKAFAHYRW"
     gene            2600..>2819
                     /locus_tag="VFR25_06070"
     CDS             2600..>2819
                     /locus_tag="VFR25_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9940648.1"
                     /translation="MPRQFDLSHMRNIGIMAHIDAGKTTLTERILYYTGKVHRMGEVH
                     DGAATMDWMEQERERGITITSAATTCFWK"
BASE COUNT          567 a          872 c          987 g          393 t
ORIGIN      
        1 gcgaggagat catcgagccg ctcggggacc gggtgctcgg ccgcgtggcc atcgaggaca
       61 tcgtggaccc gaacacgggc gacgtcatcg tccgctcggg cgaggagatc aacgaggcgg
      121 cggccgaggc gatcgaggag agcgggcgca tcgagaaggt gtcgatccgc tcggtgctca
      181 agtgcgaggc gccgcgcggc gcgtgcgcga agtgctacgg acgcaacctg gcgaccggcc
      241 gcatggtcga cctgggcgag gccgtgggcg tcatcggggc gcagtcgatc ggtgagccgg
      301 gcacgcagct cacgctccgg acgttccaca tcggtggtgt ggccggacgc atcgtcgagc
      361 agtcgaagat caaggcgaag accccgggca aggtcgtctt ccgggatctg gagacggtcg
      421 agcgctcgag tggccagctg gtcgtggtcg gccacaaggg ccagatggag atccagggtc
      481 cggacggcaa ggcgcggcag cgctactcgc ctccgtacgg cgccacgctc cacgtgcggg
      541 acgagatcga ggtgaacccg ggcgacgagc tcttcgagtg ggacacgttc agcacgccga
      601 tcctcaccga gaagccgggc gtggtgcgat tcgtcgacat caaggagaag gtcaccgttc
      661 gcgacgaggt cgacgagaac acgggcctcc ggctcatggt catcatcgac gaccgtgaga
      721 aggtgctcca tccgcacgtc gacatcatcg gcaaagggga ccgccgggtc gcgagctatc
      781 cgcttccgac cggggcgcgg ctcctcgtgc gcgacggcca cgaggtcacg gccggcgagg
      841 cgctggtcaa gatccggcgc gagatctcga agacccgcga catcacgggc ggtctgcccc
      901 gcgtgagcga gctcttcgag gcgcggaagc cgaaggacgc cgcgaccgtc agcgagatcg
      961 acgggcgcgt ggagttcggc ggcgtgaccc gaggcatgaa gaagctgatc gtggtcgggg
     1021 actcgggcga gtcccgcgag tacctgatcc cccagggcaa gcacatgcac gtccaggagg
     1081 gggactacgt ccatgccggc gaccggctga ccgagggtcc ggtcaacccg cacgacatcc
     1141 tcaagatcaa gggaatccag gcggtgcagg agtacctggt gaacgagatc caggaggtct
     1201 accgcctcca gggtgtgcgg atcgacgaca agcacatcga ggtcatcgtc cggcagatgc
     1261 tgcagaaggt tcggatcgag gacccgggcg acacgaactt cctcgaggga gagcaggtcg
     1321 acaagaccct cctcaaggtg gagcaggcca gggtggaggc cgagggtggc cagccggcga
     1381 cgtatcagcc gctcctgctt ggaattacga aggcttcgct gtcgacccag agcttcatct
     1441 cctcggcatc gttccaggag acgacccggg tgctcacgga ggcgtcggtc ctcggcatga
     1501 cggaccacct ccggggcctc aaggagaacg tcatcatcgg ccacctcatt cccgccggga
     1561 ccgggctgag ccgctaccgc ggcatcgacg tgatctcgga ggacgaagag gagaccgaaa
     1621 aagcagctcc ggtggaggaa gaggtaaaaa cagcttgacc gggggccccc gggctcgata
     1681 tagtgcccgt tctttgcgcc cgaaacctat tgtgatgagg agttttaggt gccgactctg
     1741 aaccagctcg tccggcatgg ccggaagaga cagctttcga agaccgcgtc cccggccctg
     1801 aaggcggcgc cgcagaaacg cggcgtgtgt acgcgcgtct atacatctac gccgaagaag
     1861 ccgaactcgg cgctccgcaa ggtcgcccgc gttcgcctca cgaacgggat ggaagtcacc
     1921 tgctacatcc cgggcgaggg gcacaacctg caggaacact cgatcgtcct catccggggg
     1981 ggccgtgtga aggacctccc gggcgtgcgc taccacgtcg tccggggaac cctggacgcg
     2041 tccggcgtgg acggccgtca gcagagccgg tccaagtacg gcaccaagcg gaagaagggg
     2101 gcgtaaccga tgcgccgcgc agccaagatc catcgcgtgg agctcgctcc ggacgctcgc
     2161 tacgcgaacg tgctggtcac ccgcttcatc aactgcctga tgctcggagg caagaagagc
     2221 acggccgagc gcatcttcta cggcgcgatg gacgtgatcg agaagaagac gggtcaggac
     2281 ggcttgagcg tcctgaagac cgccatgaac aacgtgaagc cggtcctgga ggtgaagtcc
     2341 cgacgcgtcg gcggcgccac ctaccaggtc ccggtcgaag tcaggcagga ccggcggacc
     2401 gcgctcgcgc tccgctggtt gatccagtac tcgcgcagcc gcccggacca cacgatggcc
     2461 gaccggctcg ccgccgagat cacggcagcc tccaagaacg aagggggcgc ggtgaagaag
     2521 agggaagaca cgcacaagat ggcagaggcg aacaaggcgt tcgcccatta ccgctggtaa
     2581 cgcgaaggac ggtttcgaag tgcccaggca gttcgatctt tctcatatgc gcaacatcgg
     2641 catcatggcc cacatcgatg ccggcaagac gaccctgacg gagcgcatcc tctattacac
     2701 ggggaaggtg caccggatgg gagaggtcca tgacggcgcg gccaccatgg actggatgga
     2761 gcaggagcgg gagcggggga tcaccatcac ctcggccgcc acaacctgct tctggaagg
//
LOCUS       DASTKC010000154         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_828050, whole genome shotgun sequence.
ACCESSION   DASTKC010000154 DASTKC010000000
VERSION     DASTKC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828050"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFR25_06075"
     CDS             <1..251
                     /locus_tag="VFR25_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET9940649.1"
                     /translation="HYKECLGGPTVRAMAKVGANSTLLPGVVIGRGALIGAGSVVTRN
                     IPDEVVAAGNPAVVIKRIDELKCHPGFFARPYEWEPYR"
     gene            complement(256..1902)
                     /locus_tag="VFR25_06080"
     CDS             complement(256..1902)
                     /locus_tag="VFR25_06080"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HET9940650.1"
                     /translation="MRRPTGTSRVWRRWASAAILGASILLPASAPATYLPNERIPTDD
                     AIYRDLERLATRYGSSARFLSSRPLRRKEALAYLAELGVEHPGAAVDPAYRRAMRDLD
                     PDAPGGVHPLLSADGDDGERIELSPYASALYAEDPRYHPEINRDYRVGLVMAGQLDTN
                     SVFLADFYEGTASQGGRGTPDFGDFNSLVQGVNFNSWVNEAYVAFPVAKVRVMFGHTW
                     LRWGPGETGTLALSDAAPALDLLHFEGDMFRSFRYQQFVSLLDPGPQTYLAGHRLEWH
                     ASPRLWVGLTEIARFDGTSQAPLYLIPFVPYSFWEKRPKTGSVDAADTSGERLAKNNV
                     AWAADASWVPHPGWRVYGEFLLDDYSFSSDYKPDMIGYQAGFEWRRMLSSRDDRRLAG
                     EGSVGSAVSSHDAAVGFLLEYSRVHNYTYSVYHHHDFAFEGFPIGYVLGPDAQLLVGQ
                     ASLEWGASWEFRARGEYRRKGEGHLGDPWLKEDGEVDASAFQGVAEKEVRLGGAIVYS
                     PSRSLELSASVGASGIENRDHVPAPEDSETPFALQAKVAW"
     gene            1956..2513
                     /locus_tag="VFR25_06085"
     CDS             1956..2513
                     /locus_tag="VFR25_06085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9940651.1"
                     /translation="MPKPIRLEGPRVTLSPFHKRDMEEFTRLNRRSRSRFVHPPTTPK
                     RFERYLTRGRAPDIFRLAVRRNEDGALLGSIEVSQISRGILQSAYLGYHIGAAFQGQG
                     YMSEAMRLVLDFSFGPLKLHRVEANIQPTNAPSIRLVKRMGFRNEGTAVRYLKIHGAW
                     QDHQRWAILAEEWRVHRRRATRRPR"
     gene            complement(2538..>2873)
                     /locus_tag="VFR25_06090"
     CDS             complement(2538..>2873)
                     /locus_tag="VFR25_06090"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET9940652.1"
                     /translation="ETAVRTMEPEDLHQLRIAAKRARYAYEYFAELEGPLAMRRAKRI
                     AGLQDFLGDHRDTTQLLRRMRRYARTVPQEDRELVLGTGSVLGHLERSSRVKRSDLRD
                     HWERAMAEI"
BASE COUNT          488 a          984 c          959 g          442 t
ORIGIN      
        1 cccactacaa ggagtgcctc ggcggaccca cggtgcgcgc gatggcgaag gtcggcgcga
       61 actcgacgct cctgcccggt gtcgtgatcg ggcgcggtgc gctgatcggc gcgggctcgg
      121 tggtgacgag gaacatcccc gacgaggtcg tcgccgccgg caatcccgcc gtcgtgatca
      181 agcgaatcga cgagctcaag tgccacccgg gattcttcgc gcggccgtac gagtgggagc
      241 cgtaccgcta ggttcctacc aggccacctt cgcctgcagc gcaaacgggg tctctgagtc
      301 ctcgggcgcc ggcacgtgat cccggttctc gatcccgctc gctcccaccg acgcgctcag
      361 ctcgagcgac cgactggggg agtaaacaat ggcaccccct agacgaacct ccttctcggc
      421 cacgccctga aacgcagagg cgtccacctc accgtcctcc ttgagccagg gatcgccgag
      481 atgaccttcg cccttcctcc gatactcgcc ccgcgcgcgg aattcccagc tcgcgcccca
      541 ctcgagcgag gcttggccca ccaggagctg cgcgtccggt ccaagcacgt agccgatcgg
      601 gaatccctcg aaagcgaagt cgtggtggtg atagaccgag tacgtgtagt tgtggactcg
      661 cgagtattcg agcaggaagc caaccgcggc gtcgtggctc gagaccgccg accccaccga
      721 tccctctccc gcgaggcgtc gatcgtcgcg cgacgacagc atgcggcgcc actcgaagcc
      781 ggcctggtag ccgatcatgt cgggcttgta atccgagctg aacgagtagt cgtcgagcag
      841 gaactcgccg taaacgcgcc accccggatg ggggacccag gacgcatccg ccgcccacgc
      901 gacgttgttc ttggcgagcc gctcgccgct cgtatccgca gcatccacgc tccccgtctt
      961 cgggcgcttc tcccagaacg agtaggggac gaaggggatc aggtagagcg gcgcctggct
     1021 ggtgccatcg aagcgggcga tctccgtgag accgacccag aggcgcggcg acgcgtgcca
     1081 ctcgagtcgg tggcccgcga gatacgtctg cgggccggga tcgaggagcg atacgaactg
     1141 ctggtagcgg aagctgcgga acatgtcccc ttcgaagtga aggaggtcga gtgcgggcgc
     1201 tgcgtcggac aaggcgagcg tgccggtctc gcccgggccc cagcggagcc atgtgtgccc
     1261 gaacatcaca cggaccttcg ccaccgggaa cgccacgtac gcctcgttca cccacgagtt
     1321 gaaattcacg ccctggacga gcgaattgaa gtccccgaag tccggcgtgc cgcgcccgcc
     1381 ctgcgatgcg gtgccctcgt agaaatcggc gaggaagacc gaattggtgt cgagctggcc
     1441 cgccatcacc aagcccacgc gatagtcccg attgatctcg gggtggtacc gcggatcctc
     1501 ggcatagagc gccgaagcgt acggcgagag ctcgatgcgc tcgccgtcgt ctccgtcggc
     1561 ggacaggagc ggatggacgc ctccgggcgc gtcgggatcg agatcgcgca tcgcgcggcg
     1621 gtaggcggga tccacggccg cgccggggtg ctccacgccg agctcggcca ggtaggccag
     1681 ggcttccttg cgccggagcg gccggctcga gagaaagcgc gcggacgacc catagcgcgt
     1741 cgcgagccgc tcgaggtcgc gatagatcgc gtcatcggtg ggaattcgct cgttgggaag
     1801 gtaggtcgcg ggagccgagg cgggcaggag gatggaagcg ccgaggatcg cagccgacgc
     1861 ccacctcctc caaaccctcg acgtccctgt cgggcgcctc atgccggcgc aggatagtcg
     1921 ctgcgatgcc aggcgtctac gctatgctgg cctccatgcc gaaaccgatc cgtctcgaag
     1981 ggccgcgcgt cacgctctcg ccgttccaca agcgggacat ggaggagttc acccggctca
     2041 atcgccggag tcggagccgg ttcgtccatc ctcccacgac gcccaagcga ttcgagcgct
     2101 atctcacgcg cggcagggcc ccggatatct tccgcctcgc cgtgcggcga aacgaggatg
     2161 gcgccctgct cggctcgatc gaggtgagcc agatctcgcg cggaatcttg cagagcgcgt
     2221 acctcggcta tcacatcggg gcggcatttc aggggcaggg ctacatgagc gaggcgatgc
     2281 gcctcgtcct cgatttctcc ttcgggccgc tcaagctcca ccgggtcgaa gcgaacatcc
     2341 agccgaccaa cgcgccttcg atccggctgg tgaagcggat gggatttcga aacgagggaa
     2401 ccgcggtccg ttatctcaag atccacggag cctggcagga ccaccagcgc tgggcgatcc
     2461 tcgccgagga gtggcgggtc catcgccgcc gcgcaacccg tcgcccgcgc tgacccgcgc
     2521 gagcgcctac cgagccctca gatctccgcc atcgcgcgct cccagtggtc gcgcagatcg
     2581 ctccgcttga cccgggacga gcgctcgagg tgcccgagaa cgctgccggt gccgagcacg
     2641 agctcgcggt cctcctgggg aacggtgcgc gcatagcggc gcatgcggcg gagaagctgg
     2701 gtcgtgtccc tgtggtcgcc gagaaagtcc tgaagccccg cgatgcgctt cgcgcggcgc
     2761 atggccagcg ggccttccag ctccgcgaaa tactcgtagg catagcgcgc ccgcttggcc
     2821 gcgatccgga gctgatgcag gtcctccggt tccatcgttc gaacggcagt ctc
//
LOCUS       DASTKC010000155         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_828139, whole genome shotgun sequence.
ACCESSION   DASTKC010000155 DASTKC010000000
VERSION     DASTKC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828139"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFR25_06095"
     CDS             <1..234
                     /locus_tag="VFR25_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HET9940653.1"
                     /translation="ACEKRPIPAERLQAIVDNLEADLLRQGSDEVKSSEIGERVMEAL
                     HQLDEVAYVRFASVYRHFKDLNQFLEELRSLLR"
     gene            248..1012
                     /gene="tatC"
                     /locus_tag="VFR25_06100"
     CDS             248..1012
                     /gene="tatC"
                     /locus_tag="VFR25_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9940654.1"
                     /translation="MIPPTLATAAVSDNQMTFLDHLDALRSSLVRAAIVAGVLIATAW
                     FFSDRLLEALIVLLAPGQKLLALGPAEAFSARMTLALWTGGLLAIPYLMYEAWRFVAP
                     GLLRDERQFALPWMVASALLLYAGIAFALELLLPTMVTMLMGFATPHVEARLQLSGLL
                     SFAVQLAGGCGLMFQLPLVLCLLVWFGIVSPKMLAGAWRHAIFFIALGAAIITPGDGP
                     SMLVLSAPLIVLYFISVGVATILWRMRGKKAREERG"
     gene            1009..1791
                     /locus_tag="VFR25_06105"
     CDS             1009..1791
                     /locus_tag="VFR25_06105"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET9940655.1"
                     /translation="MNAKGGLMLLAVDIGNTEVTLGLFEDGTLRRSFRVSSETRRTAD
                     EVRLLLGQAFPELGPGKASAVIGSVVPVQTPLYAEAARQLTGGQPLLVGSDTTAGIKI
                     EYRDPGSVGADRIANAAGAFDRYGAPAIVVDFGTATTFDVLLEGQRYLGGVIAPGVLT
                     GAENLVRRAARLGAFELKPPARVVGRTTEESLQSGVFYGAVGEVDSIVRRIAAEERIK
                     PKVIATGGLAAAIAAHSETIQEVDPDLTLYGLRIIFEKHRPG"
BASE COUNT          314 a          682 c          687 g          374 t
ORIGIN      
        1 gcctgcgaga agcgcccgat cccggccgaa aggctccagg cgatcgtgga caatctcgaa
       61 gcggacctcc tgcggcaggg atcggacgaa gtgaaatcga gcgagatcgg cgagcgcgtg
      121 atggaagccc tgcaccagct cgacgaggtc gcgtacgtgc gattcgcctc cgtctaccgg
      181 cacttcaagg acctgaatca gttcctggag gagctccggt cgctgctccg ctgagcgccc
      241 cggcccgatg atcccaccga ccctcgcgac ggccgcggtg tccgacaacc agatgacgtt
      301 cctcgaccac ctcgatgcgc tccggtcgtc gctcgtccgg gctgcgatcg tcgcgggcgt
      361 gctgatcgct accgcttggt tcttctccga ccggctgctc gaagctctga tcgtcctcct
      421 cgcgcctggg cagaagctgc tcgcgctggg tccggccgaa gcgttctcgg cgcgcatgac
      481 cctcgcgctc tggaccggcg ggcttctcgc gattccgtat ctgatgtacg aggcgtggcg
      541 cttcgtcgcc cccggcctcc taagggacga gcggcagttc gcactcccct ggatggtcgc
      601 ctcggcactc ctcctctacg cgggcatcgc gttcgcgctc gagctcctcc tgccgaccat
      661 ggtcaccatg ctcatgggct tcgcgacgcc gcacgtggag gcgaggctcc agctctctgg
      721 actcctgagc tttgccgtcc agctcgcggg cggatgcggg ctcatgttcc agctcccgct
      781 ggtgctctgt ctcctggtct ggttcggaat cgtctcgccg aagatgctgg cgggcgcgtg
      841 gcgccacgcg atcttcttca tcgcgctggg cgcggcgatc atcacgccgg gcgacggtcc
      901 gtcgatgctc gtgctctcgg ccccgttgat cgtgctctac ttcatcagcg tgggcgtggc
      961 gacgatcctg tggcggatgc ggggaaagaa ggcgcgggag gagcgaggat gaacgcgaag
     1021 gggggcctca tgctgctcgc ggtcgacatc gggaacaccg aggtgacgct gggtctcttc
     1081 gaggacggca cgcttcgccg ctccttccgc gtgagcagcg agaccaggcg caccgcggat
     1141 gaagtgcgcc tcctgctagg gcaggccttt cccgagctcg ggccaggcaa ggcgtcggcg
     1201 gtcatcgggt cggtcgtgcc cgtgcagacg ccgctctacg ccgaagccgc ccgacagctc
     1261 accggaggcc agcctctcct cgtcggttcg gacacgaccg ccgggatcaa gatcgagtac
     1321 cgggatccgg gctcggtcgg cgcggatcgc atcgcgaatg ccgccggcgc gttcgatcgc
     1381 tacggggcac cggccatcgt cgtcgacttc ggcacggcca ccacgttcga cgtcctcctc
     1441 gaagggcagc gctacctggg gggagtcatc gcgcccggcg ttctgaccgg ggcggagaac
     1501 ctggtccgcc gcgccgcgcg tctcggcgcg ttcgagttga agcctcccgc gcgcgtcgtg
     1561 ggccggacca cggaggagag ccttcagtcg ggcgtgttct acggtgcggt gggggaggtg
     1621 gattccatcg tgcggcggat cgcggcggag gagcgcatca agcccaaggt gatcgcgacc
     1681 ggtggtttgg cggcagcgat tgccgcgcac tcggaaacca tccaggaggt ggatccggac
     1741 ctgacgctct acgggctccg gatcatcttc gagaagcacc ggccggggta gtcgctcccc
     1801 gcgctcctta ccagccaggt gtacctcagt ctacatcctt agtatccctc cgaaccgtag
     1861 tcgcaagacg tttggcggac cattcttcgt ggtggcgaaa gaggtctcgc tcctttccgc
     1921 attcaggact tggaactccc cgcgcaccta aggcacgcaa gcgaatcaaa gcgaggtaca
     1981 ggctggaggt gaaacggtag acgatgattg agacgccatc tacggtggtc ttcgaaccgt
     2041 tcaagatcaa agtcgtc
//
LOCUS       DASTKC010000156         3963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2012027, whole genome shotgun sequence.
ACCESSION   DASTKC010000156 DASTKC010000000
VERSION     DASTKC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012027"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFR25_06110"
     CDS             complement(<1..961)
                     /locus_tag="VFR25_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9940656.1"
                     /translation="MSRWVMDVRYSARRLLSRPGYVVLAVLTLALGSGGLAAIISVAR
                     PLLLEPLPYQRESEVGVLWHRGDWTEQEFLFLRPQFQGFRSMAAYHAMGSTLEIPGRP
                     LRLLEGIHASAELFHVLGAQPFLGRTFRNGDDVLGAEPTAVLSYDLWRELGSDRSIVG
                     RRYSLGGEMKTVIGVMPPGFWFPSPSVQVWTAAPLDPNSRVGNYALIGRIDPTARMDR
                     MEVPLHALTARLASRFTYPAQWDKTRAPEITPVREYLVGDMKPGLLATLAGMALILLM
                     ACVNVSALMLGQVGRRSTELAVRSALGAGRRQILQQLVIEAALI"
     gene            complement(958..1686)
                     /locus_tag="VFR25_06115"
     CDS             complement(958..1686)
                     /locus_tag="VFR25_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940657.1"
                     /translation="MKTKGETLIRLDGIRKIFYTDEVETHALSGIHLEIRSGDYLSIA
                     GPSGCGKTTLLSILGLLDTATEGTYELNGRPVSGLSDIERARVRNREIGFIFQAFNLI
                     GDLTVYENVELPLSYRGIPAGERKKRAESALERVGMAHRVRHYPAQLSGGQQQRVAVA
                     RAIAGDPVILLADEPTGNLDSRNGETVMGLLRDLHRSGTTICMVTHDPRYALYAERTV
                     HMVDGQIVRDEAEARQLAAQGAEA"
     gene            complement(1707..2951)
                     /locus_tag="VFR25_06120"
     CDS             complement(1707..2951)
                     /locus_tag="VFR25_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9940658.1"
                     /translation="MDLQRTVKPKRPRYLLLAALAIVLVLATLALTRADSAVPSVKRS
                     MLTIDTVRRGPMIRQVRGPGNLAPEQIRYITTLTPGRVERILVRPGTAVVESTVLMVL
                     GNPDVNIQALQSEQQLTAAQAQLASVRLDLENQRWTQESNLASVKSECGEAVRQVEAS
                     VELDKQGMIPKLEMHRLEDRATELKTRLDVETKRLAMITASLEPQLAVQREQVRRLQA
                     IAAFQRGQVASMTVRAGAAGIVQELRLEVGQWVTSGMNLAVVAQPGRLKAVLHVPETQ
                     ARDVVLNQSAAIDTRNGIARGHVIRIDPAVQNGTVTVEAALEGKLPPGARPDMSIDGT
                     IEVERLSNVLYVGRPVLGEPESATNLFRLDPDGEHATRVPVRLGRGSVSEIEVAGGLH
                     AGDKVILSDMSEWDDHKRIRLR"
     gene            complement(3096..>3963)
                     /locus_tag="VFR25_06125"
     CDS             complement(3096..>3963)
                     /locus_tag="VFR25_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9940659.1"
                     /translation="VRRLRGLETLAARVASGDLSARIHDQSGDEIGRVAQRFDEMTEN
                     LADAKRRVEETAEQRKRLFADITHELATPLTSIRGYAETLVDPGVGTSDEERSKYLHG
                     ILEESRRMDQLIKDLFELARLEAGATPLARERIDLAALSKNTIDRFEPRFHAAGLEVS
                     WRREGDDATIVADGRRMEQVVENLLTNELRYVPSGGTVEVTLASANGRVRLAVSDDGH
                     GIAPEDRERVFERFYRAEHARPAGSEDKGSGLGLAIVREIVERHGGTVRAEARSPRGL
                     SIVIELPRASAA"
BASE COUNT          591 a         1374 c         1363 g          635 t
ORIGIN      
        1 cgatgagcgc cgcctcgatc acgagctgct ggaggatctg ccggcgcccc gcgccgagtg
       61 cggagcggac cgcgagctcc gtgctgcgcc ggccgacctg cccgagcatg agcgcggaca
      121 cgttgacgca cgccatgagg aggatgagcg ccatccctgc gagcgtcgcg aggagcccgg
      181 gcttcatgtc tccaaccagg tactcgcgca ccggggtgat ctccggtgcg cgagtcttgt
      241 cccactgcgc cgggtacgtg aaccgggaag ccagtcgcgc ggtgagcgcg tggaggggga
      301 cctccatccg gtccatccgg gccgtgggat cgattctgcc gatgagcgcg tagttgccta
      361 cgcggctgtt cggatcgagg ggcgccgcgg tccagacctg gacggacggc gacggaaacc
      421 agaagccggg cggcatgaca ccgatgacgg tcttcatctc gccgcccagg gagtagcgcc
      481 gtccgacgat cgatcgatcg gatccgagct cccgccacaa gtcgtagctc agaacggccg
      541 tgggttccgc gccgagcacg tcgtcaccgt tccggaacgt ccgcccgagg aagggctgcg
      601 cgccaaggac gtgaaagagc tccgcggacg catggattcc ctcgaggagc cggagcggcc
      661 ttcccgggat ctcgagcgtg gagcccatcg cgtgataggc ggccatggac cggaacccct
      721 ggaactgggg acgtaagaaa aggaactcct gctcggtcca gtcgccccgg tgccagagca
      781 cgccgacctc gctctcgcgc tggtagggga gcggttccag aaggagcggg cgcgcgacgc
      841 tgatgatggc cgccagtccg cccgatccca gcgcgagcgt gagcacggcg aggaccacgt
      901 atccggggcg gctcaagagc cgtcgcgccg aataccgcac gtccatgacc cagcggctca
      961 cgcttcggct ccctgcgcgg cgagctgccg cgcctcggcc tcgtcgcgca cgatctggcc
     1021 gtccaccatg tgcaccgtgc gctcggcgta gagcgcgtag cgcggatcgt gcgtgaccat
     1081 gcagatcgtc gtgccgcttc gatggagatc gcgcaagagc cccatcacgg tctcgccgtt
     1141 ccgagagtcg agatttccgg tgggctcgtc ggcgagcagg atgaccggat cgcccgcgat
     1201 ggcgcgcgcc accgccacgc gctgctgctg gcctcccgag agctgcgcgg gatagtggcg
     1261 cacgcggtgc gccatgccca cgcgctcgag cgccgactcc gcgcgcttct tccgctcgcc
     1321 cgcggggatt ccgcgatagc tgagcggcag ctccacgttc tcgtacacgg tgaggtcgcc
     1381 gatcaagttg aacgcctgga agatgaaccc gatctcgcga ttgcgcacgc gagcgcgctc
     1441 gatgtcgctc aagcccgata cggggcgccc gttcagctcg taggtgcctt ccgtcgccgt
     1501 gtcgagcagg ccgaggatcg agaggagcgt cgtcttgccg cagcccgacg gccccgcgat
     1561 cgagaggtag tcgcccgagc gaatctcgag gtgaatgccg gagagagcgt gggtctcgac
     1621 ctcgtccgtg tagaagatct tccgaatgcc gtccagccgg atcagcgtct cgcctttcgt
     1681 cttcacgatg gtctcctgga tttcgttcag cggagccgga tccgcttgtg gtcgtcccac
     1741 tccgacatgt cggacagaat caccttgtcg ccggcgtgca acccgccggc gacctcgatc
     1801 tcgctcacgg agccccgtcc caggcgcacg ggcacgcgcg tcgcgtgctc gccgtcgggg
     1861 tcgaggcgga acagattcgt cgcgctctcc ggttctccca gcaccggccg ccccacgtac
     1921 agcacgttgg ataggcgctc gacctcgatg gttccgtcga tgctcatgtc cggcctcgcg
     1981 ccgggaggca gcttgccttc cagcgccgcc tcgaccgtga cggtcccgtt ctggacggcc
     2041 gggtcgatgc ggatgacgtg ccctcgagcg atgccgtttc gcgtgtcgat cgccgcgctc
     2101 tggttgagca cgacgtcgcg ggcctgcgtc tccggcacgt ggagcaccgc cttgagccgg
     2161 cccggctgcg ccacgaccgc gaggttcatc cccgacgtga cccactggcc cacctcgagc
     2221 cgaagctcct gaacgatccc cgcggctccg gcgcgcacgg tcatggacgc gacctgcccg
     2281 cgctggaacg cggcgatcgc ctgaagccgg cgaacctgct cccgctggac cgcgagctgg
     2341 ggctcgaggg aggcggtgat catcgcgagg cgcttcgtct cgacatccag ccgcgtcttc
     2401 agctcggtcg cgcggtcctc cagacggtgc atctcgagct tcgggatcat cccctgcttg
     2461 tccagctcca ccgaggcctc gacctgacgc accgcttcgc cgcactcgct cttgaccgag
     2521 gcgaggttcg attcctgcgt ccagcgctgg ttctcgagat cgagccgcac cgatgcgagc
     2581 tgcgcctgcg cggcggtgag ctgttgctcc gactggagcg cctggatgtt cacgtccgga
     2641 ttgccgagca ccatgagcac ggtgctctcc acgaccgcgg ttcccggacg gacgagaatt
     2701 cgctcgacgc gccccggagt gagcgtggtg atgtagcgga tctgctccgg ggcgaggttc
     2761 cccggcccgc gcacctggcg gatcatgggg ccgcggcgca cggtgtcgat cgtgagcatg
     2821 gagcgcttca cgctcggcac cgccgagtcg gcccgcgtca gcgcgagtgt ggcgagcacg
     2881 agaacgatcg ccagggcggc gaggaggagg tagcgggggc gcttgggttt gacggttcgc
     2941 tggaggtcca cgcgacaggg gagggcaagc gatgtgccaa ggggatcgtg cgtgggtcaa
     3001 gcccttgcga gcctgacaca tcgtcagcgc ggtgcgacgc tagggctcga ttcgaactgc
     3061 ccggatccgg gaccgggaat cccggaatcg gacagtcagg ctgccgatgc ccgaggcagc
     3121 tcgatgacga tcgacagccc ccgcggcgac cgcgcttccg cgcgcacggt gccgccgtgg
     3181 cgctccacga tctcgcgcac gatcgcgagc ccgaggcccg agcccttgtc ctcgcttccc
     3241 gcggggcgag cgtgctcggc acggtagaac cgctcgaaga cgcgctcgcg gtcctcggga
     3301 gcgatgccgt ggccgtcgtc gctcaccgcg aggcggacgc gcccgttcgc cgaagcgagt
     3361 gtcacctcga cagtccctcc cgacggcacg tagcgaagct cgttcgtgag gagattctcg
     3421 acgacctgct ccatgcggcg accatcggcc acgatcgtgg cgtcgtcgcc ctcgcgccgc
     3481 cagctcacct cgagccccgc ggcgtggaac ctgggctcga agcgatcgat cgtgttcttg
     3541 gagagcgcgg ccagatcgat gcgctcgcgc gcgagcgggg tggctccggc ctcgagccgc
     3601 gccagctcga agaggtcctt gatgagctgg tccatccggc gcgactcctc gagaatcccg
     3661 tgcagatact tcgaccgctc ctcgtcggac gtacccacgc caggatcgac gagcgtctcc
     3721 gcgtaaccgc ggatcgacgt gagcggcgtc gccagctcat gcgtgatgtc ggcgaagagg
     3781 cgcttccgct gctccgcggt ctcttccaca cggcgcttgg cgtccgcgag attttcggtc
     3841 atctcgtcga agcgctgggc cacgcgcccg atctcgtccc cgctctggtc gtggatgcgt
     3901 gcggagagat cgccgctcgc gacgcgcgcg gcgagcgtct ccaggccgcg caggcggcgc
     3961 aca
//
LOCUS       DASTKC010000157        24423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_830614, whole genome shotgun sequence.
ACCESSION   DASTKC010000157 DASTKC010000000
VERSION     DASTKC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 24423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24423
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830614"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..280
                     /locus_tag="VFR25_06130"
     tRNA            196..280
                     /locus_tag="VFR25_06130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:230..232,aa:Leu,seq:tag)
     gene            527..1792
                     /gene="tig"
                     /locus_tag="VFR25_06135"
     CDS             527..1792
                     /gene="tig"
                     /locus_tag="VFR25_06135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9940660.1"
                     /translation="MKVEVRDSEAGVSQKVLLIEVTPEELNEDYDAILEDYRRRAVLP
                     GFRKGKAPRNVLELQFGHSMEHEVLERAVKRTYEAAVREHRIEPVSYPSIEKIRFDKG
                     QPLSYEATVDVRPTITPRDYFELNVPSQEITIPEDEVEKSIEELRQRTADWSPVEREA
                     AAGDAVVVDYVRLNAKGKPVHKSEQKDALVELDAQGLLPEFRENLLGKRGGEHIEFTV
                     TYPEEFGNAELKGRTATFSLDVKGVRERTVRPLDDAFASEVIGMRDLAELRARMRLNL
                     EGEARMKARRDEEEAIVDQLLAKNPIALPESMVAEYLEELLQRLKDDREWTAEEEERF
                     RTEYRPTAERRIKRDLLLDAIMRAESIQVSEEEIDQTLRGAMGEETTGPEAERAMRNP
                     AQRERARSHLLERKLFQLLREKAQLKITA"
     gene            1822..2466
                     /gene="clpP"
                     /locus_tag="VFR25_06140"
     CDS             1822..2466
                     /gene="clpP"
                     /locus_tag="VFR25_06140"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET9940661.1"
                     /translation="MDTREDTRMSLVPIVVEQSGRGERAYDIFSRLLKDRIIFIGTPI
                     DDVVANLVIAQLLFLEAEDPDRDINLYINSPGGVVSAGLAIYDTMQFIKSDVATICMG
                     QAASMAALLLAAGAAGKRSALPHSRIMIHQPLGGSQGQATDIEIYTKEILTLRTELNK
                     ILVRHTGQPVDKIEKDTDRNFFMSSEEAKAYRIVDKVIAKLETVKKQPEPVTIG"
     gene            2483..3736
                     /gene="clpX"
                     /locus_tag="VFR25_06145"
     CDS             2483..3736
                     /gene="clpX"
                     /locus_tag="VFR25_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET9940662.1"
                     /translation="MKKRVSSPHPIRCSFCGRGQDEVSRLVSGPSVYICSECVKLCND
                     ILEGETDHTVGAPKGSLPKPAEIKKVLDDYVIGQDRAKKTLSVAVYNHYKRIYSGNSV
                     DDVELEKSNILLIGPTGTGKTLLARTLARILKVPFAIVDATCLTEAGYVGEDVENILV
                     RLLQATDFNVVNAEHGIVYIDELDKISRKSENPSITRDVSGEGVQQALLKILEGTIAN
                     VPPQGGRKHPQQKYIEVNTKDILFICGGAFEGLEKIVERRVSQKAMGFGAEIKSRKER
                     NLGEFLSLVEPDDLLRYGLIPELVGRLPVTGALDSLSKASLIDILTKPKNAITKQYQK
                     FFEMEGVKLEFTEDGLEAVAEAALKRGTGARGLRAVLEESMLDLMYEIPSRLEVMGCT
                     ITREVIERKGPPILAHRADRRRKEA"
     gene            3741..6185
                     /gene="lon"
                     /locus_tag="VFR25_06150"
     CDS             3741..6185
                     /gene="lon"
                     /locus_tag="VFR25_06150"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9940663.1"
                     /translation="MTRVERDGEVFEIRERIPLLPLRDVVIYPYMTIPLLVGRVASVN
                     AIEAAVQKDRVVFVLAQKRPEVTDPAPKDLYRVGTVVRVLQLFRLPDGTMRVLVEGIC
                     RGKVKKFAKDGDTMAASVTVLHDEVRAEPQLEALIRNVLATFNDYVHLNRRVPDEILA
                     TANNIANPLLLSYTIASHLILKVGVKQEILEQDDLVERFRLLSRTLASELEILKLERK
                     IEGQVRSQVHKNQKEFYLNEQLKAIRKELGYQNEFSNEIEELTQQVKKARMPKEVHDK
                     TMKELDKLQKMSFMSPEATVVRNYVDWMVSLPWSIATDDNVDIKEVERILDEDHYGLK
                     KVKERIVEYIAVLKLSGKLKGPILCFVGPPGVGKTSLGKSIARALGRKFVRMSLGGVR
                     DEAEIRGHRRTYIGSLPGRIIQGMKRAGTKNPVFLLDEIDKLGMDFRGDPASALLEVL
                     DPEQNFSFQDHYIEADFDLSQVMFITTSNSLYSIPGPLVDRMEIIRLPGYLEHEKVEI
                     AKKFLIPKECVAHGLETSDLRIGDKALRAIINQYTREAGVRSLEREIAAICRKVARKK
                     ATLDKPRPFHITEPRVHQYLGVPKYLDSQVERRSRIGVATGLAWTDSGGDVLTVEVSV
                     VPGSGALLLTGKLGETMRESGQAALSYTRSRATALGLDKNFYKEIDVHVHLPEGAIPK
                     DGPSAGITMATALVSALTGVPTRDDVAMTGEITLLGNVLPIGGLNEKVVAAIRAGVKE
                     VILPKLNEKDLKELPETVRHGVKFRLVETMDEVLQIAMNPPPVVAPEIAAGDLAGGVS
                     DEPPAEPGDEQVPLAH"
     gene            6232..7392
                     /locus_tag="VFR25_06155"
     CDS             6232..7392
                     /locus_tag="VFR25_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET9940664.1"
                     /translation="MKRVLYTPGPTQVPTEVLEAMARPLIHHRTDEYRQQFLEATRLL
                     AEHLGTKQPVLTLSCSGSGAMEAAAVNLLSPGDEVIVVEGGKFGNRWGSIAEAYGVTV
                     HKVSVAWGRVATIEEVESALTAHPKAKAMFLTHSETSTGALFPAHEIARLGRARGVLT
                     VIDAVTSFGVYDLRFDDSDIDGVVWGSQKGLMIPPGLGYVCYSPKAWAVAEKAKLPRF
                     YFNLVKARGALEKGDTPFTPAINLVLGSHAAMKIMDREGRLGVFERHQRNADATRHAV
                     RTLGLPLFAEVPSNALTAVEVPAGVDGGAVVKTMEQRYGVKIAGGQDQLKGKIFRLGH
                     IGYYDDGDILRLVGAFESALIDHGFRAEAGTAVRAAQQSLRGAKRPEPARVG"
     gene            7424..8350
                     /locus_tag="VFR25_06160"
     CDS             7424..8350
                     /locus_tag="VFR25_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET9940665.1"
                     /translation="MTAPKRVLVSDPLSPRAIFMLRSAPGLSVEERKGLGEADLLPLV
                     TDIDAWIVRGATKVTRRLLEASSQLRWVARAGAGLDNIDVAAAKERGIDVLNVPGANA
                     VAVVELVFGLLLGLMRHIPAADASVHRGEWDKSKLMGRELRGKTIGIVGLGKIGRGVA
                     QRARAFEMTCVGCDPLVPDDAIRAMNVEPLPLDDLLERAEILTLHVPLGGETKGMISA
                     ERLALLPRGAILVNAARGGLVDEAALVAALDSGAIAGAGLDVFANEPPGDSPLARHPR
                     VVATPHIGAATVEAQEAVGEEIVRLLLARMGG"
     gene            8622..9239
                     /gene="thrH"
                     /locus_tag="VFR25_06165"
     CDS             8622..9239
                     /gene="thrH"
                     /locus_tag="VFR25_06165"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HET9940666.1"
                     /translation="MIAAIDLEGVLAPEIWPYLGDVFGLPELHLTTRDTADFDDLMSQ
                     RVGALSRAGLTLAKVQEAAHRVQPYLGSKEFLHRIRRHCQVMIISDTFHEFAEPMMER
                     LGGYNLFANRFEIDKAGHISGWKLRIRGRKAMMIDGMRHAGFKVIGMGDSLNDLTLLQ
                     MADHPILFRPVEKLRAELPQAPSVQTLDEALELFMGIFRKNGSVA"
     gene            9251..9640
                     /locus_tag="VFR25_06170"
     CDS             9251..9640
                     /locus_tag="VFR25_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940667.1"
                     /translation="MEYRVREEIAPEQLNALISASWEKPWTGDWSSILARSLTWITAY
                     RDGSLVGFVHVAWDGGAHAFLLDPTVHPSMRRTGIGTELVRMAARSARDSGVEWLHVD
                     YEPHLEPFYAACGFGPTQAGLIRLAGM"
     gene            10263..11546
                     /locus_tag="VFR25_06175"
     CDS             10263..11546
                     /locus_tag="VFR25_06175"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9940668.1"
                     /translation="MSCRVVVGAQWGDEGKGKIVDLLSQDADIVARYQGGPNAGHTVC
                     IGDRQFVLHLIPSGILRPRTVCYIGNGVVVDPEALRRERETVLKQGISVDGRLFVSGS
                     AHVILPDHRAVEKVTETGTTPIGTTGRGIGPAYTEKAGRTGLRVVDLLDRTIREERVH
                     ALRERTLRMAPGAELEPIEAVLARCEKDAEMLRPITANVSREINAALAAGKRVLLEGA
                     QGTHLDLDHGTYPYVTSSSAVSGGACTGVGIGPTHIDEVIGVAKAYATRVGNGPFPSE
                     LHGELADRLREEGNEYGATTGRPRRCGWFDVVAIRHAVRVNGLTQLVITKLDVLDGLS
                     EVHAVTAYDIGGVHTAEMPESLADLMAARPITERFPGWRSPTTDARQWDDLPPDARKY
                     LDRLEEMAGVPIRLVSVGSGREENVERRARHAVGR"
     gene            11500..12177
                     /locus_tag="VFR25_06180"
     CDS             11500..12177
                     /locus_tag="VFR25_06180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9940669.1"
                     /translation="MKRTWSAAPGTPSGDSSVRAPFTPAPVTLAGEHVRLEPLSLEHH
                     LDPLASIALDPDLWRWTLNVVETRDDLRRYLEEALREQAEGRSVPFATIHLATGRAAG
                     CTRFGNIDARHRKVEIGWTWVGRDFQRTRVNTEAKLLMLQHAFETWGCHRVELKTNVL
                     NERSRNAMLRIGAQEEGILRKHALNDRGVPRDTVYYSVIDDEWPAVKARLRAMLAERR
                     EPKPAEA"
     gene            12400..13290
                     /locus_tag="VFR25_06185"
     CDS             12400..13290
                     /locus_tag="VFR25_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940670.1"
                     /translation="MPDSLPDFDARWDYDHPDSTEAVFRALLPQARASGNKDYLAQLL
                     TQIARTQGLQMKFDDATKTLEEAQALLTPDMRVARVRVLLERGRVWNSSKHQDFAIPL
                     FTAAWDLAREAGALGQPGADGYAVDAAHMLAIAQPGDLSLQWNQTAIAYAEHSQDPKA
                     RKWLGSLYNNEGWDYHGKGDYPKALEIFKKALEARRAEGDPAKIRIAEWCVARTLRSL
                     QRNDEALEIQKRLLSEAQAAGQPDGFIFEEMGECLIALQRPEEARPYFAVAYEMLSKD
                     PWLKRDEPERLERIRKFAGL"
     gene            13371..13955
                     /locus_tag="VFR25_06190"
     CDS             13371..13955
                     /locus_tag="VFR25_06190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9940671.1"
                     /translation="MARHGETEWSLAGRHTGRTDIDLTPRGEEDARRLGERLQELTLA
                     AIFTSPSKRAVRTCALAGFANRAVPDPDLAEWDYGKYEGLRSAEIRIERPSWELFHDG
                     CPGGESPSDIGARADRIVAKVRAIQGDVLLVSSAHFLRVLTARWLGLDPSAGQYFVAS
                     PAGLGCLGYEHDISEPAVRLWNDTVHLADPARPS"
     gene            14174..16582
                     /locus_tag="VFR25_06195"
     CDS             14174..16582
                     /locus_tag="VFR25_06195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET9940672.1"
                     /translation="MATAWAQEGGHHGGEANLVLPDLSSVTFVGGMNGHTLLLFGLIV
                     CAFGLLFGLLTYSQVRKMPVHESMREISELIYETCKTYLLQQGKFLILLWVFISAIVL
                     VYFGWLAVSGVDAAGNVVRGFPPFKVAVILFFSLVGMAGSYSVAWFGIRINTLANSRT
                     AFASLTGKPYGCYAIPLRAGMSIGMALISIELLIMLIILLFVPGDMAGPCFIGFAIGE
                     SLGAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGPSA
                     DGFETYGVTGVALITFILLAVQDATIQVQLLVWIFAMRVMMVVASGLSYQINEAWSKA
                     QYVNADKMDFEAPLTRLVWLTSFVSIALTYVASNLLIHDLGDGTLWWKLSTVITCGTL
                     AGAIIPELVKIFTSMHSKHVTEIVTSSREGGASLNILSGFVAGNFSAYWLGLTILALM
                     AAAYGVSTLGLASIMIAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSQ
                     IETIPGIKEKVRSEFGFDVDFEKAKHNLEENDGAGNTFKATAKPVLIGTAVVGATTMI
                     FSIIVGLTEGLKPEYVANLSLLHAPFLLGLITGGAVIYWFTGASTQAVTAGAYRAVEF
                     IKQNIKLEGVTKASVSDSKKVVEICTQYAQKGMFNIFLAVFFGTLAFAFVEPFYFIGY
                     LVSIALFGLYQAIFMANAGGAWDNAKKVVEVVLKEKGTPLHDATVVGDTVGDPFKDTS
                     SVAMNPVIKFTTLFGLLAVDLAVSLTRQSGPVLTSVLAAIFLLISMTFVWRSFYAMRI
                     TAAK"
     gene            16725..17306
                     /locus_tag="VFR25_06200"
     CDS             16725..17306
                     /locus_tag="VFR25_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9940673.1"
                     /translation="MNEDRWERLLRLLGPIHEQAAGTARRLSRSADEGDDLYQESVLR
                     AYERLHDLRDESKFRSWFFATLLTIHRSRRRRRATVSLEEAWPKGGEPAGEDGSTWAE
                     ALERANRAARALDGLAPVQREALVLHEIEGFSVEEVAAMQGVTISAVKSRLVRGRDKL
                     RQFYERILKSARIPGLRRETEPARELAPGGEIR"
     gene            17303..18331
                     /locus_tag="VFR25_06205"
     CDS             17303..18331
                     /locus_tag="VFR25_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940674.1"
                     /translation="MNDPNAPDELRADLAALKKVSDRDLPDLKHVMNEISRRRAVEAA
                     PIMGWRRRIMAAVQTMKARPALAALAAAVVLIAVGMVVPVSYDRVTGHDVSISLAGAG
                     FTPDQIAPTAKEMKARLGGESVQVEATEDNGAARFVFHTTLPERSRAEAARKAGAFVR
                     ELAQKGIAASLTVAPHKEHVTSPAVAYAWNQIIQISVDGKSASALESEIKQRLAEAGV
                     PDAQVSVKDGGAGGREISVNVQRQNFGGAPAGQQPEPELVLTKNGAPITGGSTVRIKK
                     MKDQTGATSLVVETSDHGKTATATIPNSDTMSDAQLQSAIETQLRQAGLNVRVEVVGG
                     QIKVRPVE"
     gene            18534..19793
                     /locus_tag="VFR25_06210"
     CDS             18534..19793
                     /locus_tag="VFR25_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940675.1"
                     /translation="MIGARTGEGWQARMRPRGIGRFFPAAFLSFWLCGWAAGEAFALW
                     MLFRGLKALLTGDPGGGELPVPVPFILPMGAFLIFWLAVWTVGGITAMGELLRLTWSE
                     DRIVAGPGGITLHTSRGPFRKRVEIPRDEIREVIPSARGSALAVVTPRSTIVLTRNGS
                     AAEREEVAVMLRSELGLRDQTPGASLTWNPGMAPMELTGLPSGWEDAISPEGERILMR
                     SAKSRATGARVVGVIALGMATLAAFVVRKALGSFALTPPAVIMTAFAVGLAWLTFWLA
                     MARSEWKIGSGRIVLRQRFRSTVRDVFEADRLELTFSSDSDGDERFALMALKGASASI
                     TGSAFFTDPKCRRLVDSSLRDPAGLRRLGAWLAQSAGIPFDDRTGAASRQADIAALTA
                     QLNLSGPLGRFAAKLVTRATAKVKKSA"
     gene            complement(19830..20294)
                     /locus_tag="VFR25_06215"
     CDS             complement(19830..20294)
                     /locus_tag="VFR25_06215"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9940676.1"
                     /translation="MSPAPEVEKIAPIRKSVVVRRKPEEAFSIFTERLSSWWPHKTHS
                     LFKDKAATVVMEPRNGGKLYEISDSGERKEWGTIVSWDPPRGFVSTWHPGMDPANPTE
                     VEIRFTAVSEGTRVDLEHRNWEKLGAKAAESREGYDKGWVAVFEESFVKGCA"
     gene            complement(20291..20656)
                     /locus_tag="VFR25_06220"
     CDS             complement(20291..20656)
                     /locus_tag="VFR25_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9940677.1"
                     /translation="MAYQQAIQALADPTRRLVFERLRGGPLPVGRVARGLRVTRPAVS
                     QHLRVLERAGLVSAEKDGTRRLYRIEHRGLEDLRRYLDRFWDDVLESFRLEAEHGVES
                     KSSSRTPTPPASRRKRRMR"
     gene            21307..22473
                     /locus_tag="VFR25_06225"
     CDS             21307..22473
                     /locus_tag="VFR25_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9940678.1"
                     /translation="MTGPLTFDRAVSLAARYQLGLRAADLRASASHARIRDAGRRPNP
                     VLLGTGENFGGDLGSDRLESTLEIAQTIELGGDRGARSAVAAGESRLAAADAAVLRRG
                     LLMLSAERFARAWSLQERLVRLREGEDLTRQAIAAASARYRAGASPLVERARAESQAL
                     AQAVARQQAQSELAIARLELAQMWGAAEAAFDSLVAVMPAREPETVPRLAPHPELEQA
                     NATEELSGARRKAAEAMRVPDVTFSGGVRHLEEVSGTGFLAGVELPIPLWNRSAGTVD
                     AARREQEAAVAERRAVERRLDVEVSNAAERVRAAAATYDTLTLRLRPARQDLVTELLR
                     FYRAGRLSYLDLIAEQRFLLETDLAVVDAQADLWLSQIRLDLLTGASSLQGGGR"
     gene            22470..23420
                     /locus_tag="VFR25_06230"
     CDS             22470..23420
                     /locus_tag="VFR25_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9940679.1"
                     /translation="MNAAGIRNTWMVALVVCLTVTGCQKREAHEEEHEAEHEVGTHGG
                     ALVELSDEAARTAGIAVDSVGPRAIGVVVELPGEIKLDAERSVEVRPSYPGRILQLHA
                     GLGAFVRKGQPLVSIYSNESLSEYTIEAPMTGTVVARPVNPGAAVDPASVIYTLANLS
                     SVWLDFPIYVRYLGSIHQGQTVRVQSDENTGTFATGTISYIGPMLDVDTRATFGRVVL
                     PNADRRWQPGRLVTASVILERVTAPMAVPEEAIVREGSGAAVFRATPQGFELQPVTPG
                     RSDGVMTEILSGLEPGARVATRNAFLLKAELEKEAGGHED"
     gene            23410..>24423
                     /locus_tag="VFR25_06235"
     CDS             23410..>24423
                     /locus_tag="VFR25_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9940680.1"
                     /translation="MRTDPGLPEARSTLDRILRFSILHRRSIVVMTAVVAAIGIVSLA
                     HIPIDAVPDITTVQVQINTVVEGLPPLDIERQVTFPIETAMAGLPGLTETRSLSRYGL
                     SQVTVVFRDGTSIYFARQLVNERLQEARDALPAGIAEPRMGPIATGLGDIFLWEVEAD
                     PGARKADGTAYSVTDLREIQDWVIKPQIRTVPGVTEVNTIGGYRKQYDVRPDPARLRA
                     YGLTTGDVLLALERNNQAVGAGYLPRKGEQYLVHTDGRLSRPEEIEQVVVATRSGVPL
                     HVADVATVGLGQELRTGAATSHGRETVVGTAIMLFGENSRVVANRVGERLKEVNRSLP
                     SGVT"
BASE COUNT         4169 a         8172 c         8155 g         3927 t
ORIGIN      
        1 tcgacatgtg ccggaagctg ctcctcgagg cgcgggacct ggtcgagggg gcgtacctca
       61 tgccttcctt tggccgctac gaagtcgtgg cgcgtgttgc cgaagccgtc ctagaccggc
      121 agtcgtcgta gccgcagcag cccaatcctc tggaaccaca agcacttcca tactagattg
      181 accacgggta cccgagcgag agtggcggaa ctggcagacg cgcgggactt aggatcccgt
      241 gaggaaactc gtgggggttc gactcccccc tctcgcacca aaaatccgct ccaagacagg
      301 cgcttcgacg cgggctccgg ggatcaatgg ccctcggtcc agaccgccct gggtccccgg
      361 gccgaaatct gctagaatgc gggcctcaga cacgcgatag tgccctgata gccgcgaatt
      421 tagggccgat ttggtcctcc tggcgccccg gcgcatctca aggggccaca ccgagagaac
      481 cgagccggcc cgtccaatgc cctaacctat acggggagaa gagattttga aggtcgaagt
      541 ccgcgacagc gaagctggag tctcccagaa ggtcctcctg atcgaggtca cgcccgagga
      601 gctgaacgag gattacgacg ccatcctcga ggattaccgg cgccgggcgg ttctgccggg
      661 gttccgtaag gggaaggccc cgcggaacgt cctggagctc cagttcggcc actccatgga
      721 gcacgaggtc ctcgagcggg cggtcaagcg cacgtacgag gcggccgtgc gggagcacag
      781 gatcgagcct gtctcgtacc cgtcgatcga gaagatccgg ttcgacaagg ggcagccgct
      841 ctcctacgag gccacggtcg acgtgcggcc cacgatcacg ccgcgcgact acttcgagct
      901 gaacgtgccg tcgcaggaga tcacgatccc cgaggacgag gtggagaagt cgattgagga
      961 gctacggcag cgcaccgccg actggtcgcc cgtcgagcgt gaagccgccg ccggagacgc
     1021 cgtggtggtg gactacgttc gcctgaacgc gaaggggaag ccggtccaca agtccgagca
     1081 gaaggacgcg ctggtcgagc tggatgccca ggggctcctg cccgaattcc gcgagaatct
     1141 cctcggaaag aggggcggag agcacatcga gttcacggtg acctatcccg aggaattcgg
     1201 gaacgcggag ctcaagggac ggacggcgac cttctccctc gacgtgaagg gcgtgcggga
     1261 gcggaccgtc cgcccgctcg acgacgcgtt cgcgagcgag gtgatcggca tgcgcgacct
     1321 ggccgagctt cgagcccgga tgcgactcaa cctcgagggt gaggcccgca tgaaggcccg
     1381 ccgggacgag gaggaggcca tcgtcgacca gctcctggcc aagaatccca tcgccctgcc
     1441 cgagagcatg gtggcggagt atctggagga gctcctccag cgtctaaagg acgaccgcga
     1501 gtggaccgcc gaggaggaag agcgcttccg gaccgagtac cggccgacgg ccgagcgacg
     1561 gatcaagcgc gacctcctgc tcgacgcgat catgcgggcg gagtcgatcc aggtttccga
     1621 ggaggagatc gatcagaccc tccgcggggc catgggtgag gaaaccacag gcccggaggc
     1681 cgagcgggcc atgcggaatc cggcccagcg ggagcgggca cggagtcacc tcctcgagcg
     1741 gaagcttttc caactgctcc gggagaaagc gcagcttaag ataacggcat gattttccga
     1801 tcaatcggtc gggacgcacc aatggatacg agggaggaca cacggatgtc gctggtaccg
     1861 atcgtggtgg aacagagcgg gcgcggggag cgggcctacg acatcttctc ccggctcctc
     1921 aaggaccgga tcatcttcat cgggacgccg atcgacgacg tggtcgcgaa cctcgtcatc
     1981 gcccagctcc tcttcctcga ggccgaggat ccggatcgcg atatcaacct ttacatcaac
     2041 tcgcccggcg gcgtggtctc ggcagggctc gcgatctacg acacgatgca gttcatcaag
     2101 tcggacgtcg cgacgatctg catggggcag gcggcctcga tggcggcgct cctcctggcc
     2161 gcgggcgcgg caggaaagcg ctcggcgctt ccgcactcgc gtatcatgat ccaccagccg
     2221 ctcggcggga gccagggaca ggcgaccgac atcgagatct acacgaagga aatcctgacg
     2281 ctccggacgg agctcaacaa gattctggtg cgccacaccg gccagccggt ggacaagatc
     2341 gagaaggaca ccgaccgcaa cttcttcatg tcctccgagg aagccaaggc gtaccgcatc
     2401 gtggacaagg tcattgccaa actcgaaacc gtgaagaaac aaccagagcc ggtcacgatc
     2461 ggctgaggac accaaggaat ccatgaagaa gcgcgtcagc tcgccacatc ccatccggtg
     2521 cagcttctgc ggccgcggcc aggacgaggt ctcccgcctc gtgtcgggtc cgtccgtcta
     2581 catctgcagc gagtgcgtca agctctgcaa cgacatcctc gagggtgaga ccgaccacac
     2641 ggtcggcgcc cccaagggct cgctcccgaa gccggccgag atcaagaagg tcctggacga
     2701 ctacgtgatc ggccaggacc gtgccaagaa gacgctctcc gtcgccgtct acaaccacta
     2761 caagcgcatc tactcgggca actccgtcga tgacgtcgag ctcgagaagt cgaacatcct
     2821 gctcatcggc ccgaccggca cggggaagac gctcctcgcg cggacgctcg cgcgcatcct
     2881 caaggtgccg ttcgccatcg tcgacgcgac ctgcctcacc gaggccggct acgtgggcga
     2941 ggacgtggag aacatcctcg tccggctcct ccaggcgacc gatttcaacg tggtgaacgc
     3001 ggagcacggg atcgtctaca tcgacgagct cgacaagatc tcccggaagt ccgagaaccc
     3061 ctcgatcacg cgcgacgtgt cgggcgaggg cgtgcagcag gcgctcctca agatcctcga
     3121 gggcacgatc gcgaacgtcc cgccgcaagg cgggcggaag catccgcagc agaagtacat
     3181 cgaggtgaac accaaggaca tcctcttcat ctgcggcggc gcgttcgaag ggctggagaa
     3241 gatcgtggag cggcgcgtga gccagaaggc gatgggcttc ggcgccgaga tcaagagccg
     3301 gaaggagcgg aacctgggcg agttcctctc gctcgtggag ccggacgatc tccttcgcta
     3361 cggactcatt cccgagctgg tggggcggct cccggtgacc ggcgcgctcg attcgctgag
     3421 caaggcgtcg ctcatcgaca tcctcaccaa gccgaagaac gcgatcacga agcagtatca
     3481 gaaattcttc gagatggaag gcgtgaagct cgagttcacc gaagacggcc tggaagccgt
     3541 cgccgaggcg gccctgaagc gcggaaccgg cgcgcgcggc ctccgggcgg tgctcgagga
     3601 gtccatgctc gacctcatgt acgagatccc ttcgcggctc gaagtgatgg gctgcaccat
     3661 cacgcgggaa gtcatcgaga ggaagggacc accgatcctc gcccaccggg cggatcgccg
     3721 acgcaaggag gcgtgagcgt atgacgcgcg tcgaacgcga cggcgaagtc ttcgaaattc
     3781 gggaacgcat tccgctcctc ccgcttcgcg acgtcgtcat ttatccctac atgaccatcc
     3841 cgctcctcgt gggccgggtt gcctcggtga acgcgatcga ggcggccgtc cagaaggatc
     3901 gggtcgtatt cgtgctcgcc cagaagcggc ccgaggtcac cgaccccgcc ccgaaggacc
     3961 tctaccgggt cgggaccgtg gtgcgcgtgc tccagctctt ccggctcccg gacgggacga
     4021 tgcgcgtgct ggtcgaggga atctgccgcg gcaaggtgaa gaagttcgcc aaggatgggg
     4081 acacgatggc ggcctccgtc acggtgctcc acgacgaggt ccgcgccgag ccgcagctcg
     4141 aggcgctgat ccggaacgtg ctcgccacgt tcaacgacta cgtccacctg aaccgccgcg
     4201 tgccggacga gatcctcgcg accgcgaaca acatcgcgaa tcctctgctc ctttcctaca
     4261 ccatcgcgtc gcacctgatc ttgaaggtgg gcgtgaagca ggagatcctc gagcaggacg
     4321 atctggtcga gcgcttccgg ctgctcagca ggacgctcgc gagcgagctc gaaatcttga
     4381 agctcgagcg gaagatcgag gggcaggtcc ggagccaggt tcacaagaac cagaaggagt
     4441 tctatctcaa cgagcagctc aaggcgatcc gcaaggagct gggctaccag aacgagttct
     4501 cgaacgagat cgaggagctc acccagcagg tcaagaaggc gcggatgccg aaggaagtcc
     4561 acgacaagac catgaaggag ctcgacaagc tccagaagat gtccttcatg tcgcccgagg
     4621 cgacggtcgt ccgcaactac gtcgactgga tggtctcgct cccctggagc atcgcgaccg
     4681 acgacaacgt cgacatcaag gaagtggagc gcattctcga cgaggaccac tacggcctga
     4741 agaaggtcaa agagcggatc gtcgagtaca tcgcagtctt gaaattgtcc ggaaagctga
     4801 agggtccgat cctctgcttc gtcggccctc ccggcgtcgg caagacctcg ctcgggaagt
     4861 cgatcgcgcg agcgctggga cggaagttcg tccggatgtc gctcggcggc gtgcgggatg
     4921 aggccgagat ccggggacat cgacggacgt acatcggatc gcttcccggg aggatcatcc
     4981 aggggatgaa gcgggcgggg accaagaacc ccgtgttcct gctggacgag atcgacaagc
     5041 tcggcatgga cttccgcggc gatcctgctt cggcgttgct cgaggtgctg gatccggagc
     5101 agaacttctc gttccaggac cactacatcg aggccgactt cgacctctcg caggtcatgt
     5161 tcatcacgac ctcgaactcc ctctattcga ttcccggacc gctcgtggat cgcatggaga
     5221 tcatccggct cccgggctac ctggagcacg agaaggtcga gatcgcgaag aagttcctga
     5281 tccccaagga gtgcgtcgcg cacgggctcg agacgtcgga tctcaggatc ggcgacaagg
     5341 ctctgcgcgc gatcatcaac cagtacaccc gcgaggcggg cgtccggagc ctggagcgcg
     5401 agatcgcagc catctgccgc aaggtcgcgc gcaagaaggc cacgctcgac aagccgcgtc
     5461 cgttccacat cacggagccg cgcgtgcacc agtacctcgg cgtgccgaag tacctcgaca
     5521 gccaggtcga gcgccggtcg aggatcggcg tcgcgaccgg actcgcctgg accgattccg
     5581 gcggcgacgt gctgacggtg gaggtcagcg tcgtgccggg gtcgggcgca ctcttgctga
     5641 cagggaagct gggcgagacg atgcgcgagt cggggcaggc ggcgctgtcg tacacgcgct
     5701 cccgcgccac ggcgctcggg ctcgacaaga acttctacaa ggagatcgac gtccacgtgc
     5761 acctgcccga gggagcgatc ccgaaggatg gtccgtcagc gggcatcacg atggcgacgg
     5821 cgctggtgtc ggctttgacg ggcgttccca cgcgggacga cgtcgcgatg acgggcgaga
     5881 tcacgctgct cgggaacgtg ctcccgatcg gcggcctcaa cgagaaggtc gtggccgcga
     5941 tccgcgcggg cgtgaaggaa gtcattctcc ccaagctgaa cgagaaggac ctgaaggaat
     6001 tgcccgagac ggtgcgtcac ggagtgaagt tccggctcgt cgagacgatg gacgaggtgc
     6061 tccagatcgc gatgaatccg ccgcccgtgg tcgctcccga gatcgcggcg ggcgatcttg
     6121 caggcggcgt gtccgacgag cctccggcgg agccggggga cgagcaggtc ccactcgcgc
     6181 attgacacct gagaacccca tcggggttct cttgactcag gagagccccc gatgaagcgc
     6241 gttctctaca ctcccggccc gacccaggtg cctaccgaag tcctggaggc gatggcgcgg
     6301 ccgctgatcc accaccgcac cgacgaatac cgccagcagt tcctcgaggc gacccgcctt
     6361 ctggccgagc acctgggcac gaagcagcca gtgctcacgc tctcctgctc cggcagcggc
     6421 gcgatggaag ccgcggcggt caatctactg tcgcccggcg acgaggtgat cgtcgtcgag
     6481 ggcgggaaat tcgggaatcg ctggggctcg atcgccgagg cgtacggcgt gaccgtgcac
     6541 aaggtgagcg tggcgtgggg acgcgtggcg acgatcgagg aggtcgagtc cgcgctgacc
     6601 gcgcatccca aggccaaggc gatgttcctc acgcacagcg agacctcgac gggcgctctc
     6661 ttcccggcgc atgagatcgc gcggctcggg cgcgcccgcg gggttttgac cgtgatcgat
     6721 gctgtgacga gcttcggggt ctacgacctg aggttcgacg actccgacat cgacggcgtg
     6781 gtgtggggct cccagaaggg cctcatgatt ccgccgggcc tcggctacgt ctgctactcg
     6841 cccaaagcgt gggctgtggc cgaaaaggcg aagctgccgc gcttctactt caatctcgtg
     6901 aaggcgcggg gcgctctgga gaagggcgac acgccgttca ctccggcgat caacctggtg
     6961 cttggatccc acgccgcgat gaagatcatg gatcgcgagg gtcgcttggg cgtgttcgag
     7021 cggcatcagc ggaacgcgga cgccacgcgg cacgcggtgc gcacgctcgg gctgcccttg
     7081 tttgccgaag tgccttcgaa tgcgttgacc gcggtcgaag tccccgcggg cgtggatggc
     7141 ggcgccgtcg tgaagacgat ggagcagcgc tacggcgtca agatcgcggg cggccaggac
     7201 cagctcaagg ggaagatctt ccggctcgga cacatcggct actacgacga cggcgacatc
     7261 ttacgccttg tcggcgcgtt cgaatcggcg ttgatcgatc acggattccg cgccgaagcg
     7321 ggcacggccg tgcgggcggc gcaacagtcg ctgcgcggcg caaagcgccc cgagccggcg
     7381 cgagtcggtt gagccggcgc ttgctggcgg tgggcgggcc cgcgtgacgg ctcccaagcg
     7441 cgttctcgtc agcgatccgc tgtccccgcg cgccatcttc atgctgcgct ccgctcccgg
     7501 cctttccgtc gaggagcgca agggcctcgg cgaggccgat ctccttcctc tcgtcaccga
     7561 catcgacgcc tggatcgttc gcggcgcgac caaggtcacg cgccgcttgc tcgaagcctc
     7621 ctcgcaattg cgctgggtcg cccgcgccgg cgccggactc gacaacatcg acgttgccgc
     7681 cgcgaaggag cgggggatcg acgtcttgaa cgtgcccggt gcgaatgccg tggccgtggt
     7741 ggagctcgtg ttcgggctct tgctcgggtt gatgcgccac attcccgctg cggacgcttc
     7801 cgtccatcgc ggcgagtggg acaagtcgaa gctgatgggg cgcgagctcc gggggaagac
     7861 gatcgggatc gtcggactgg gaaagatcgg acgcggcgtg gcgcagcgcg ctcgggcgtt
     7921 cgagatgacc tgcgtgggct gcgatccgct cgtgccggat gatgcgatcc gcgcgatgaa
     7981 cgtggagcct ctgccgctgg acgatctcct ggagcgtgcg gagattctga cgctccatgt
     8041 gcccctgggc ggagagacga aagggatgat ctcggccgag cgtctggcgc tcctcccgcg
     8101 cggcgcgatc ctcgtgaacg ccgcccgcgg cgggctcgtg gacgaggcgg cgctcgtggc
     8161 cgccctggac agcggagcca tcgcgggcgc cgggctggac gtcttcgcga acgagccccc
     8221 cggggactcc ccgctggcgc gccacccacg tgtggtcgcc acgccccata tcggagccgc
     8281 gacggtggag gcccaggagg cggtgggcga ggagatcgtg cggctgctcc tggcccggat
     8341 gggcggctag gggcggctga gggcaacggc ccgacggcgg ctggcggccc aaaaaacttt
     8401 tgacaaggtc ctccgatcgc gcgagcatcc cttcatcgcg gaaaggaggt ggtccattgc
     8461 atattagtgg cagacggatc agcggaggtg gctgtagcta gcgctacatc cagcgcgctc
     8521 tagcctggtg tggtctggcg aatcccaaca gcccaccgga gttttgaggg gagaggtcgg
     8581 aaggcctctc cccgctcttt tgtagccccc gaaaggcccc gatgattgcc gcgatcgatc
     8641 tggaaggcgt gctggcccct gagatctggc cctacctcgg cgacgtgttc gggctccccg
     8701 agctccacct cacgacgcgg gacacggccg atttcgacga cctcatgagc cagcgcgtcg
     8761 gcgccctctc ccgagccggc ctgaccctgg cgaaggtcca ggaggccgcg caccgggtgc
     8821 agccctacct cgggtccaag gagttcctcc accggatccg gcggcactgc caggtcatga
     8881 tcatctccga caccttccac gagttcgccg agcccatgat ggagcgcctc ggcggctaca
     8941 acctctttgc gaaccgcttc gagatcgaca aggccggcca catctcgggc tggaagctcc
     9001 ggatccgcgg ccgcaaggcc atgatgatcg acggcatgcg gcacgccggc ttcaaggtga
     9061 tcgggatggg agacagcctg aacgatctca cgctgctgca gatggccgac cacccgatcc
     9121 tgttccgccc cgtcgagaag ctgcgcgccg agctaccgca ggctccgtcc gtccagaccc
     9181 tggacgaagc gctggagctc ttcatgggga tcttccggaa gaacgggagc gtggcgtagg
     9241 gctcgtgggg ctggagtatc gcgttcgaga agagatcgct ccggagcagc tgaacgcgct
     9301 gatctccgct tcctgggaga agccctggac cggcgactgg tcctccatcc tggcccgaag
     9361 tctgacctgg atcactgcgt atcgggacgg cagtctcgtc ggcttcgtcc atgtggcctg
     9421 ggacggcggt gcacacgcgt ttctcctcga cccaaccgtg cacccctcca tgcgaaggac
     9481 gggcattggt accgagctcg tacggatggc cgcccggtcg gcgcgcgact ccggtgtcga
     9541 atggctccac gttgactacg agccccatct ggagccgttc tacgctgcat gcggtttcgg
     9601 accgacccaa gcaggactca tccggctcgc ggggatgtga ctctcagatc atctggcccc
     9661 ggggccagtt gatcgtcaac cttcgttaac gcatttcccc cgtcaacagg ccccggggcc
     9721 agttgatccc caaccttcgc ctcacgcctc ttcgaccatc aacaggcccc ggggccagtt
     9781 gatcccaacc ttcgcctacg cctctccgac cctcaacagg ccccggggcc agttgatcgt
     9841 caacttcgcc tcacgcctct ccgaccatca acaggccccg gggccagttg atccccaacc
     9901 ctcatttacg ccgcagctgt gcgacagcac cgaggtgtca cagccaatag cctgcaaaat
     9961 tggaatttgc ctcacgaccg cagttacgca cggatgcatt gttgctccgc gccgacatgg
    10021 tcccgcggcg agtccaacag gccccggggc ctgttcgatc actgccgtcc gccccagccg
    10081 gcccccgccg ccctcgccag cccgcctcac cagcccgccc cccgcgaacc ggccctccgg
    10141 ccggcccgcc ccccgcattg actcaccacc ccccctctgg taggttcggt cggccgcatc
    10201 aagccgttcc cgcccgccga aggaaggccc ttccgcggac tttttcattt ggaggcatca
    10261 ccatgtcttg ccgtgttgtg gtcggcgccc agtggggtga cgaggggaag gggaagatcg
    10321 tcgacctttt gagccaggac gcggacatcg tcgcccggta ccaggggggc cccaacgcgg
    10381 ggcataccgt ctgcattggc gaccgccagt tcgtcctgca tttgatcccg tccgggattc
    10441 ttcgtccccg cacggtctgc tacatcggaa acggcgtcgt ggtcgatccc gaggcgctcc
    10501 ggcgcgagcg ggagaccgtc ctcaagcagg gaatctcggt cgacggccgg ctcttcgtgt
    10561 ccggctcggc gcacgtgatc cttcccgatc accgcgccgt cgagaaggtc accgagaccg
    10621 gcaccacgcc gatcggcacg accgggcgcg gcatcggccc cgcctacacc gagaaggcag
    10681 gacgcacggg gctccgcgtc gtcgatctcc tcgaccgcac gatccgcgag gagcgcgtcc
    10741 acgcgcttcg cgagcgcacg ctcaggatgg cgcctggcgc ggagctggag ccgatcgagg
    10801 cggtgctcgc gcgctgcgag aaggacgcag agatgctccg gcccatcacc gcgaacgtct
    10861 cccgcgagat caacgcggcg ctcgcggccg ggaagcgcgt gctcctcgaa ggagcgcagg
    10921 gaacgcatct cgacctcgat cacggaacgt atccgtacgt cacgtcgtcc agcgcggtct
    10981 cgggcggcgc ctgcaccggc gtcggcatcg gtccgacgca catcgacgag gtgatcggcg
    11041 tcgcgaaggc gtacgccacg cgtgtcggca acggcccgtt cccgtcggag ctccacggcg
    11101 agctggcgga ccggctgcgc gaggaaggga acgagtacgg cgccacgacg ggccgtccgc
    11161 gccgctgcgg atggttcgac gtggtcgcga tacgtcacgc cgtgcgcgtg aacgggctca
    11221 cgcagcttgt catcacgaag ctcgacgtgc tcgacggact ctccgaggtg cacgcggtca
    11281 ccgcctacga catcggcggc gtgcacacgg cggaaatgcc ggagtcgctc gcggatctca
    11341 tggcggcgcg acccatcacc gagcgcttcc ccgggtggcg ctcgcccacg accgacgcgc
    11401 gccagtggga cgatctcccg ccggatgcgc gcaagtatct cgatcgtctc gaggagatgg
    11461 cgggcgttcc gatccgcctc gtctctgtgg gatccggacg tgaagagaac gtggagcgcc
    11521 gcgcccggca cgccgtcggg agatagctcc gtgcgggctc cgttcacacc ggctcccgtc
    11581 acgctggccg gggagcacgt ccgcctcgag cccctctcgc tcgagcatca cctcgatcct
    11641 ctcgcctcga tcgcgctcga tcccgatctt tggcgctgga ccctgaacgt cgtcgagacc
    11701 agggacgatc tccgccgata cctggaggag gcgctccgcg agcaggccga gggccgctcc
    11761 gttcccttcg ccaccatcca cctcgccacc ggtcgtgccg ccggatgcac gcgcttcggc
    11821 aacatcgacg cccggcatcg caaggtcgag atcggatgga cctgggtggg acgcgatttc
    11881 cagcgcacgc gcgtgaacac cgaagccaag ctcctcatgc tccagcacgc gttcgagaca
    11941 tggggctgcc atcgcgtgga gctgaagacg aacgtgctga acgagcgctc ccggaacgcg
    12001 atgctccgga tcggcgccca ggaggagggc atcctccgca agcacgccct gaacgaccgt
    12061 ggcgtgccgc gcgataccgt ttattacagc gtgatcgatg acgagtggcc cgcggtgaag
    12121 gcgcgtcttc gcgcgatgct cgccgagcgt cgcgaaccga agccggccga ggcgtgagcg
    12181 gtggcgtcgc gggtcgtcca agcgttgctc gcggccctgg cggtggcgat cgttgtcgca
    12241 cctccggcgt cggccggccc gaagcccgcg gccaagtcag ccacgaccac acgggcgtcc
    12301 gcaaaagccg cggccccgac accagagccg gcgagccccg ccaacccggc cagccccgcc
    12361 aacccggcca acccggccaa cccggccaac ccgcaatcca tgcctgactc gctccccgat
    12421 ttcgacgcgc gctgggatta cgaccatccc gactcgaccg aagccgtctt ccgcgcgctc
    12481 ttgccccagg cgcgcgcgag cgggaacaag gactatctcg cgcagctcct cactcagatc
    12541 gcgcggacac agggactcca gatgaagttc gacgacgcca cgaaaacgct cgaggaagcg
    12601 caggcgctcc tcacgccgga catgcgcgtc gcgcgcgtgc gcgtgctgct cgagcgaggg
    12661 cgggtctgga acagctcgaa gcaccaggac ttcgcgatcc cactcttcac ggccgcgtgg
    12721 gatctggctc gcgaagccgg ggcgctgggc cagccggggg cggacggata tgccgtggac
    12781 gcggcgcaca tgctcgccat cgcacagccg ggagacctct cgctccagtg gaatcagacc
    12841 gccatcgcat acgcggagca ttcgcaggat cccaaggccc gcaagtggct cggctcgctc
    12901 tacaacaacg aaggatggga ctatcacggc aaaggggact atccgaaggc gctcgagatc
    12961 ttcaagaagg cgctcgaggc gcggcgcgcg gaaggtgacc cggcgaagat tcggatcgcg
    13021 gagtggtgtg tggctcgaac gcttcgctcg ctgcagcgaa atgacgaagc gctcgagatc
    13081 caaaagcggc tcctgtccga ggcgcaggca gcgggccagc cggacggatt catcttcgag
    13141 gaaatgggtg agtgcttgat cgcgctccaa cgccccgagg aagcgcgccc ctacttcgcc
    13201 gtggcctacg agatgctctc caaggacccg tggctcaagc gcgacgagcc ggagcgcctc
    13261 gagcgcatcc ggaaattcgc agggctgtga cgccgcccgg cgcaacgcgg gcggccgcgc
    13321 cgcccggcgc aacgcggacg gccgcgccgc aaaaactccc cgccgtctgg atcgcgcgcc
    13381 acggcgagac cgagtggagt ctggcgggcc gccacacggg ccgcaccgac atcgatctca
    13441 ctccccgcgg cgaggaagac gcgcgccgac tgggcgaacg actccaagag ctcacattgg
    13501 ctgcgatctt caccagcccg tcgaagcgcg cggtccgcac gtgcgccctg gcgggcttcg
    13561 ccaatcgggc cgtgcccgac ccggacctcg ccgaatggga ttacggcaag tacgaaggcc
    13621 tgcgctcagc cgagattcgg atcgaacggc cgagctggga gctcttccat gacggatgtc
    13681 ccggagggga gtcgccgtcc gacatcggtg cacgagcgga tcggatcgtg gccaaggttc
    13741 gcgcgatcca gggcgacgtc cttctcgtat cgagcgcgca ctttctgcgc gtcctcaccg
    13801 cgcgctggct gggacttgac ccatcggccg ggcagtactt cgtcgcgtcg ccggccggtc
    13861 tcgggtgcct cggatatgag cacgacattt cggagccggc cgtccgcctc tggaacgata
    13921 cggtgcacct cgccgacccg gcccggcctt cctgacccct cgacaggcat ccccggaact
    13981 gctataaacc tgggttcttg cacggcaccc acttgagacg ggcgatgaag cgtctgtggc
    14041 ccccacaggc ggccaacccc caagcacaca ctcgaatcga ggagacatga tgctgggaaa
    14101 ttggtccaga ggacgggcga tgctcgcggc tctcatcctg gcggcggtgg cgcttgcggc
    14161 cccgttcgcg cccatggcga cggcctgggc tcaggaaggc ggtcatcacg gcggggaggc
    14221 caacctggtc ctgcccgacc tctcttccgt gacgttcgtg ggaggtatga acgggcacac
    14281 gctgctcctg ttcggcctca tcgtctgcgc gttcgggctc ctcttcgggc tcctcacgta
    14341 ctcgcaggtc cggaagatgc cggtccatga gtcgatgcgc gagatctcgg agctgatcta
    14401 cgagacgtgc aagacctacc tcctccagca gggcaagttc ctcatcctcc tctgggtctt
    14461 catcagcgcg atcgtgctgg tctacttcgg ctggctcgcc gtctcgggcg tggacgcggc
    14521 ggggaacgtg gttcgcgggt tccccccctt caaggtcgcc gtcatcctct tcttcagcct
    14581 cgtcggcatg gcggggagct actcggtcgc gtggttcggc atccggatca acacgctggc
    14641 caactcccgg accgcgttcg cgtcgctcac cgggaagccc tacggctgct acgcgatccc
    14701 gctccgcgcg ggcatgagca tcggtatggc gctcatctcg atcgagctcc tcatcatgct
    14761 catcatcctc ctcttcgttc cgggagacat ggcgggcccc tgcttcatcg ggttcgcgat
    14821 cggtgagtcc ctcggcgcgg ccgcgctccg aatcgccgga ggcatcttca cgaagatcgc
    14881 cgacatcggc tcggacctca tgaagatcgt cttcaagatc aaggaagacg acgcgcggaa
    14941 tcccggcgtg atcgcggact gcaccggcga caacgcgggc gactccgtcg ggccgtcggc
    15001 cgacgggttc gagacctacg gcgtgacggg tgtcgcgctc atcacgttca tcctcctggc
    15061 cgttcaggac gcgacgatcc aggtgcagct cctggtctgg atcttcgcga tgcgcgtcat
    15121 gatggtcgtg gcgagcggcc tgtcgtatca gatcaacgag gcctggagca aagcccagta
    15181 cgtgaacgcg gacaagatgg acttcgaggc tccgctcacc cgtctcgtct ggctcacctc
    15241 gttcgtgtcg atcgcgctca cgtacgtggc gtccaacctg ctcatccatg acctgggcga
    15301 cggcacgctc tggtggaagc tctcgaccgt gatcacgtgc ggcacgctcg cgggggcgat
    15361 catccccgag ctggtcaaga tcttcacctc gatgcactcg aagcacgtga ccgagatcgt
    15421 gacctcctcg agggagggtg gcgcgtcgct caacattctc tccggcttcg ttgcgggcaa
    15481 cttcagcgcc tactggctcg gcctcacgat cctcgcgctc atggctgcgg cgtacggcgt
    15541 gagcacgctt ggtctcgcgt cgatcatgat cgccccggcc gtgttcgcgt tcggcctcgt
    15601 cgcgttcggc ttcctgggca tgggtcccgt gacgatcgcg gtcgactcgt acggccccgt
    15661 gacggacaac gcgcagtcgg tctacgagct ctcgcagatc gaaacgatcc ccgggatcaa
    15721 ggaaaaggtg cgctccgagt tcggcttcga cgtggacttc gagaaggcga agcacaacct
    15781 cgaggagaac gacggggcgg ggaacacctt caaggcgacg gccaagccgg tgctcatcgg
    15841 cacggcggtc gtgggcgcga ccacgatgat cttctcgatc atcgtcggac tcacggaagg
    15901 cctgaagccc gagtacgtgg ccaatctctc gctcctccac gccccgttcc tgctcggcct
    15961 catcacgggc ggcgcggtga tctactggtt caccggcgcg tcgacgcagg cggtgaccgc
    16021 cggcgcctac cgcgcggtcg agttcatcaa gcagaacatc aagctcgaag gggtcacgaa
    16081 ggcgtcggtg tcggattcga agaaggtggt ggagatctgc acgcagtacg cgcagaaggg
    16141 catgttcaac atcttcctcg cggtcttctt cggcacgctg gcctttgcct tcgtcgagcc
    16201 gttctacttc atcggctatc tcgtctcgat cgcgctcttc ggcctctacc aggcgatctt
    16261 catggccaac gccggcggcg cctgggacaa cgcgaagaag gtggtcgagg tcgtactcaa
    16321 ggagaagggg acgccgctcc acgatgcgac ggtcgtcggc gatacggtgg gcgatccgtt
    16381 caaggacacg tcctcggtcg ccatgaatcc ggtcatcaag ttcacgacgc tcttcgggct
    16441 cctggcggtc gatctcgccg tgtcgctcac gcggcagtcg ggccccgtgc tcacgagcgt
    16501 cctcgcggcg atcttcctcc tgatctccat gaccttcgtg tggcgctcgt tctacgccat
    16561 gaggatcacg gcggcgaagt aacgctgccg tcgtaagcca cggcctaggc cttggttgtc
    16621 accgaagggc ccctctccgg agattcttgc gacccggagg ggggtccttt gcgtctccgg
    16681 tacgaacacc cgatcgggag ggatgaacca ggttggacgc tcgcgtgaac gaggatcgct
    16741 gggaacgcct gctccggctg ctcggaccga ttcacgagca ggccgccggc acggcccgcc
    16801 gcctctcgag gtcggcggac gaaggcgacg acctctacca ggagtccgtg ctgcgcgcgt
    16861 acgagcggct tcacgacctg cgcgacgagt ccaagttccg gtcatggttc ttcgccacgc
    16921 tcctcacgat ccatcgatcc cgccggcgcc ggcgggcgac ggtgagcctc gaggaggcct
    16981 ggccgaaggg cggagagccg gcgggcgagg acgggtcgac gtgggcggag gctctcgaac
    17041 gcgcgaatcg ggcggcacgg gccctggatg gactggcgcc ggtccagcgc gaggccttag
    17101 tcctccacga gatcgaagga ttctcggtgg aggaggtcgc ggcgatgcaa ggggtcacga
    17161 tctcggcggt gaagtcgcgg ctcgtccgcg gccgggacaa gctgcggcag ttctacgaac
    17221 gaatcttgaa gtcggcccgg atccccgggc ttcgcaggga gaccgagccc gcgcgcgagc
    17281 tcgctcccgg aggagagatc cgatgaacga tccgaacgcg ccggacgagc tccgggccga
    17341 tctcgcggca ctcaagaagg tcagcgaccg ggatcttccg gatctgaagc acgtcatgaa
    17401 cgaaatcagc cggcgtcggg ccgtggaggc cgcgccgatc atgggatgga ggaggaggat
    17461 catggcagcc gttcaaacga tgaaagcccg ccccgcgctc gcggcgctcg ccgccgcggt
    17521 ggttctgatc gcggtcggga tggtggttcc ggtgtcctac gaccgcgtga cggggcacga
    17581 cgtctcgatc tcgctcgccg gcgccggctt cacgccggat cagatcgcgc cgacggcgaa
    17641 ggagatgaag gcgcgcctgg gcggggagtc ggtccaggtg gaagcgacag aggacaatgg
    17701 cgccgcgcgg ttcgtctttc acacgacgct gcccgagcgc tctcgcgccg aggcggcgcg
    17761 gaaggccggc gcgttcgtga gggagctagc ccagaagggc atcgcggcgt ccctgaccgt
    17821 ggcgccgcac aaggagcacg tcacgtctcc agccgtcgcg tatgcgtgga atcagatcat
    17881 ccagatcagc gtggacggga agtcggccag cgcgctcgag tcggagatca agcagcgtct
    17941 cgccgaagcg ggcgtgcccg acgcgcaggt ctccgtgaag gacggcggcg cgggtgggcg
    18001 cgagatctcc gtgaacgtgc agcgccagaa cttcggcgga gcgccggcgg ggcagcagcc
    18061 tgagcccgag ctcgtgctca cgaagaacgg tgcgccgatc accggcggat caaccgtgag
    18121 gatcaagaag atgaaggatc agaccggcgc gacgtcgctc gtggtcgaga cctccgatca
    18181 cggcaagacc gcgaccgcga cgatcccgaa ctccgacacg atgtccgacg cgcagctcca
    18241 gagcgcgatc gagacccagc tccgtcaggc ggggctgaac gtgcgggtcg aggtcgtggg
    18301 cgggcagatc aaggtgcggc cggtcgagta acgttggcgc gcgcgaagcg cgcggaacgc
    18361 gtacagcagg gcgccccggg gcctccaggt tcggggcgct ctggtttgtg gcgccaggtg
    18421 aacctcaggc ctgatgtagt tgaaataatg tgacttgcgt ccagtcctgt gaatgcctcc
    18481 cgaatcccgc ccaagccctc ctcaaccatc cccaatcagc cgatgatttc ctcatgatcg
    18541 gagcgcgcac aggggagggc tggcaggcgc ggatgcgccc tcgaggcatc ggaagattct
    18601 tcccggccgc gttcctctcc ttctggctgt gtggatgggc ggcaggagag gcattcgcgc
    18661 tctggatgtt gttccggggc ctgaaggcgc tcttgacggg ggatccgggc ggcggagagt
    18721 tgccagtgcc cgtccccttc atcctcccga tgggcgcgtt tctcatcttc tggctggcgg
    18781 tctggacagt gggcggcatc accgccatgg gcgagctcct ccgtctcacg tggtcggagg
    18841 atcgcatcgt cgcgggaccc ggaggcatca cgctccacac gtcgcgcggt cccttccgga
    18901 agagagtcga gatccctcgc gacgagatac gcgaggtcat tccctcggcg cgcggctcgg
    18961 cgctcgccgt cgtgacgcca cgcagcacga tcgtgctcac gcggaacgga tcggccgcgg
    19021 agcgggagga agtggcggtc atgctccggt cggagctggg actccgggat cagacccccg
    19081 gcgcttcctt gacatggaac ccggggatgg cgccgatgga attgaccggg cttccttccg
    19141 gctgggagga cgcgatctct ccggagggcg agcgcatcct catgcggagc gcgaagagcc
    19201 gcgccaccgg ggcgcgtgtc gttggcgtga tcgcgctcgg aatggccacc ctggcggcat
    19261 tcgtcgtgcg caaggcactg ggcagcttcg cgctgactcc gcccgccgtg atcatgacgg
    19321 cgttcgccgt cgggctcgcg tggctcacgt tctggctcgc catggcacgt agcgagtgga
    19381 agatcggcag cggccgcatc gtcctgcgcc agcgattccg aagcacggtc cgcgacgtgt
    19441 tcgaggcgga ccgcctcgag ctcacgtttt ccagcgacag cgacggggac gaacgattcg
    19501 cgctcatggc cctgaagggc gccagcgcgt cgatcaccgg ctctgcattc ttcaccgacc
    19561 ccaagtgccg ccgcctcgtc gactcatccc ttcgtgatcc ggccgggctc cggcggctcg
    19621 gcgcatggct cgcccagtcc gccgggatcc cgttcgacga tcgcaccggc gccgcctcgc
    19681 gccaggccga catcgcggcc ctcacagcac agctcaatct ctcgggtccg cttggtcgtt
    19741 ttgctgcgaa gctcgtgacc cgagccaccg cgaaggtgaa gaagagcgcg tagcggcgcg
    19801 cctgcaccgc cacgcagctc cgtaccctcc tacgcgcacc ctttcacgaa gctctcctcg
    19861 aacaccgcga cccagccctt gtcgtagccc tcgcggctct ccgcggcctt cgcacccagc
    19921 ttctcccaat tcctgtgctc cagatccacg cgcgtgcctt cggacacagc cgtgaagcgg
    19981 atctcgacct cggtcgggtt cgccgggtcc atccccggat gccaggtcga gacaaatcct
    20041 cgcgggggat cccaggacac gatcgtgccc cactccttgc gctcgcccga gtctgagatc
    20101 tcgtagagct tcccgccgtt tcttggctcc atgacgaccg tagccgcctt gtccttgaac
    20161 agcgagtgcg tcttgtgcgg ccaccaggac gagagacgct cggtgaagat ggagaacgcc
    20221 tcctcgggct tgcggcggac caccaccgat ttccggatgg gagcgatctt ctcgacttcc
    20281 ggggctgggc tcatctcatt ctcctcttgc ggcgggatgc cggcggggta ggggtcctgg
    20341 acgaagactt ggattcgacg ccgtgctcgg cttccaagcg gaacgactcg agcacgtcgt
    20401 cccagaaacg atccagatag cgacgaaggt cctcgagccc tcggtgctcg atgcggtaga
    20461 gccgccgcgt gccgtccttc tccgcgctga cgagccccgc gcgttcgagc acccgaaggt
    20521 gctgcgagac cgcggggcgg gtgacgcgaa gcccgcgcgc gacgcgcccg acggggagcg
    20581 ggccgcctcg aagcctctcg aacacgaggc ggcgggttgg atcggcgaga gcctggatcg
    20641 cttgttggta agccatgact aacagtaaac cacggcttac cgatcaagtc aagccctttt
    20701 cctccccttg acgccagtcg ccggttcatc aagactgcgt ccatggtccg tgggtcgatc
    20761 cttcgttttc tggtcatggt gcaggccgtt cttctggtcc cgccgctcct gctcgggtcg
    20821 gtctgcctga aggagggcag tgccaccctc gagcccgtga cctgctcctg ctccattccc
    20881 gcgtccttga ctccatctca ggtgcctggc acccacgtcg agcaggattg cggcccgtgc
    20941 cgcgacgtga ccctctctgc cctcggttcg cgcgcacagc agtccatgtc tccggacgcc
    21001 ctgctccttc atccttcctc gctgcagccg ctggtcaacg cctccaggcc ggatcccacc
    21061 gcgcccgaac cctcgggcag tcttcccgaa cggagcttct cggtccttcg ctgctgatct
    21121 ccagggctct ctgagttcta cggggcgcgc ctgacgcgcc ctccgtgatg ttcccgagcc
    21181 caaggagatc gttctgtgct ctcgccatgg agatttgctc tgtgctgcgc cgcggccctg
    21241 gcgctggtcc aggcggcggc cgcgcctgta cgcgcggacc aggccgcaat cgacagtgtt
    21301 cccgtcgtga ccggacccct gacgttcgat cgcgccgtgt cgctcgcggc ccggtaccag
    21361 ctcggcctgc gcgccgccga tctcagggcg agcgcgtcgc acgcgcggat ccgcgacgcc
    21421 ggccgccggc ccaacccggt tctcctcgga acgggcgaga actttggcgg cgacctgggc
    21481 tccgatcggc tggagagcac gctcgagatc gcacagacga tcgagctggg aggcgaccgc
    21541 ggtgcccggt cggcggtcgc cgcgggcgag tcgcgcctcg cggccgcgga tgcggcggtc
    21601 ctccggcgcg gtcttctcat gctgtccgcg gagcgtttcg cgcgcgcctg gtcgctccaa
    21661 gaacgcctgg tccgcctgcg tgagggagag gacctcaccc gtcaggccat cgcggcggcg
    21721 tccgcccggt accgggcagg cgcgtccccg ctggtcgagc gtgctcgcgc cgagagccag
    21781 gcgctggcgc aggcggtagc acgccagcag gcccagtccg agctcgccat cgcgcggctg
    21841 gagctcgcgc agatgtgggg cgccgccgag gcggcgttcg actctctggt cgccgtgatg
    21901 ccggctcgcg aaccggagac cgtcccccga ctggccccgc atcccgagct ggaacaggca
    21961 aacgccacag aggagctctc cggagcgcga cggaaagcgg ccgaggccat gcgcgttccc
    22021 gacgtgacgt tctcgggagg ggtgcggcat ctcgaggagg tctcgggcac cgggttcctc
    22081 gccggcgtcg agcttcccat tcccttgtgg aaccgatccg cagggactgt cgacgcggca
    22141 cggcgcgagc aagaggccgc cgtcgcggaa cggcgtgcgg tggagcggcg tctcgacgta
    22201 gaggtgtcga acgcggccga gcgcgttcgg gcagcagccg caacctacga cacgctcacc
    22261 ttgcgcctcc gtcccgcacg gcaggatctg gtcacggagc tgctccgctt ctatcgagcc
    22321 gggcgcctca gctacctcga tctgatcgcg gagcaacgat tcctactcga gacggatctg
    22381 gcggtggtcg acgcccaggc cgacctgtgg ctctctcaaa ttcggctcga tctcctgacc
    22441 ggagcctcct cgctccaggg aggcggacga tgaacgcggc cgggatccgg aacacgtgga
    22501 tggtggcgct ggtcgtctgt ctcacggtga ccggctgtca gaagcgagaa gcgcacgagg
    22561 aggagcatga ggcggagcac gaggtgggga cccacggtgg ggctctcgtc gagctctcgg
    22621 acgaggcggc gcgcaccgcg ggcatcgccg tggacagcgt cggtccgcgg gcgatcggcg
    22681 tcgtggtcga gctcccgggt gagatcaagc tcgacgcgga gcgaagcgtg gaggtccggc
    22741 cgagctatcc gggacgaatc cttcagctcc acgccggcct cggcgctttc gtacgcaaag
    22801 gacaacctct ggtttcgatc tacagcaacg agagcctgag cgagtacacg atcgaagcgc
    22861 cgatgaccgg gacggttgtg gcgcgtccgg tcaatcctgg agccgcggtc gatcccgcgt
    22921 cggtgatcta cacgctcgcc aatttgagct ctgtctggct cgacttcccg atctacgtcc
    22981 ggtatctcgg atcgattcat caggggcaga ccgtgcgcgt ccagagcgac gagaacaccg
    23041 gcacgttcgc gacaggaacc atcagctaca tcggtccgat gctcgatgtg gacacgcgcg
    23101 ccacctttgg ccgtgtcgtc ttgccgaacg ccgaccggcg ttggcagccc ggccgcctcg
    23161 tgaccgcctc ggtgatcctg gaacgcgtca cggcgccgat ggcggtaccc gaagaggcga
    23221 tcgtgcgcga ggggagcggc gcggccgtgt ttcgcgcgac cccgcaagga ttcgagcttc
    23281 agcccgtcac gccgggacga agcgacggcg tgatgaccga gatcctctcg ggtctcgagc
    23341 ccggcgcgcg cgtggccacc cggaacgcct tcctgctcaa ggcggagctc gagaaggagg
    23401 cgggcgggca tgaggactga cccaggcctg cccgaagcgc gatcgaccct ggaccggatc
    23461 ctccgcttct cgatcctcca ccgccggtcg atcgtcgtca tgacggcggt cgtggccgcg
    23521 atcgggatcg tgagcctggc gcacattccg atcgacgctg tgcccgacat cacgacggtc
    23581 caggtgcaga tcaacaccgt cgtggaaggg ctcccgccgc tcgacatcga gcgccaggtg
    23641 acctttccga tcgagaccgc catggccggg ctcccaggcc tgaccgagac gagatcgctg
    23701 tcgcgctacg gcctctctca ggtcaccgtc gtgttccggg acggcaccag catctacttc
    23761 gcgcgccagc tcgtgaacga gcggctccag gaggcccgcg acgcgttacc cgcaggcatc
    23821 gccgagccga ggatggggcc gatcgccacc ggcctcggcg acatcttcct ctgggaggtc
    23881 gaggccgatc cgggcgccag aaaagcggac ggcaccgcct acagcgtgac cgaccttcgc
    23941 gagatccagg actgggtcat caagccccag atccggaccg ttcccggcgt gaccgaggtg
    24001 aacacgatcg ggggataccg caagcagtac gacgtgcggc cggatccggc gcgtctccgc
    24061 gcgtacgggc tcacgaccgg cgacgtcctc ctcgcgctcg agcgcaacaa ccaggcggtg
    24121 ggagccggct accttccccg caagggggag cagtacctcg tgcacaccga cgggcggctg
    24181 tcccggccgg aggagatcga gcaggtcgtc gtggcgacgc gaagcggcgt gcccctgcac
    24241 gtggccgacg tcgcgacggt tggactcggt caggagctgc gcaccggtgc ggcgacgagc
    24301 cacggtcggg agacggtcgt gggcaccgcg atcatgctct tcggagagaa cagccgcgtg
    24361 gtggccaatc gcgtggggga gcgcttgaag gaagtgaacc gctcccttcc ttcgggcgtg
    24421 acc
//
LOCUS       DASTKC010000158         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_833632, whole genome shotgun sequence.
ACCESSION   DASTKC010000158 DASTKC010000000
VERSION     DASTKC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833632"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="VFR25_06240"
     CDS             complement(<1..1714)
                     /locus_tag="VFR25_06240"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET9940681.1"
                     /translation="MTHPFLKRVQQGVVLADGAMGTYLYEKGIPFDRSFDLLNLTDAS
                     LIQAVHREYIRAGAEVIETNTFGGNRYRLAAHGATETARRVNRAGAQIARNAREEVGE
                     AVFVAGSMGPLGKPVAPLGTITREEAYDAYREQAEGLVEGGVDLIILETQTDMAEALV
                     AIDAVRSVAGDVPLVVQMTYTEDGRTLYGSYPEDVVHAVLEKKVDLLGANCSVGPQKL
                     LEIVERLKRRTEVPISVMPNAGLPRFVHGRFVYLSSPEYLAEYAQRFAAAGARLVGGC
                     CGTTPTHTRKMRDALRAAAGAPAPLAPSPPGAGPAIVIEETTAKTDPTSRSTLARKLA
                     AKEFVVSVEVDPPRGARPKKMIEGAQFLKDAGVDFINVADSPMARVRMSSIALASMIG
                     HQVGIETILHFTCRDRNLMGIQSDLMGAHALGIRNILALTGDPPRAGDYPNATAVFDV
                     DSIGLIGVLKRLNAGTDLGGNSIGEPTTFVIGCAVNPTAETLDEELDRFQRKMEAGAE
                     FAMTQPLYELATLTQFLDKLGKMGKKRVPILLGLLPLQSHRHAEFLHNEVPGIHVPDH
                     AREAM"
     gene            1837..>2258
                     /locus_tag="VFR25_06245"
     CDS             1837..>2258
                     /locus_tag="VFR25_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HET9940682.1"
                     /translation="MNIVVLVKQVPDTETRIQVKDGSVDLSGVKWIVNPYDEFAIEEA
                     LRIREKLGQGKVTAVSLGPDRVKEAIKYALSLGSDEGVWVKGDGVALGDPLAVATVLA
                     AAAKKIGFDLVLTGKQGVDSDWSQVGPMVAELLDLPHVS"
BASE COUNT          333 a          776 c          764 g          385 t
ORIGIN      
        1 gcatggcctc gcgggcgtgg tccgggacgt ggatgccggg cacctcgttg tggaggaact
       61 ccgcgtggcg gtggctctgg agcggcagaa gcccaagcag gatgggcacc cgcttcttcc
      121 ccatcttccc gagtttgtcg aggaactggg tcaaggtcgc gagctcgtac agaggctgcg
      181 tcatcgcgaa ctcggccccc gcctccatct tcctctggaa gcggtccagc tcctcgtcga
      241 gcgtctccgc ggtcggattc accgcgcacc cgatgacgaa tgtggtgggc tcaccgatcg
      301 agttcccgcc gagatcggtg cccgcgttga gccgcttcag caccccgatc aagccgatcg
      361 agtccacgtc gaacaccgcg gtcgcgttgg gataatcccc cgcccgcggc gggtcgccgg
      421 tcagcgcgag gatgttccgg atcccgagcg cgtgcgctcc catgagatcg gactggatgc
      481 ccatgagatt tcgatcgcgg caggtgaagt ggaggatcgt ctcgatcccg acctggtgcc
      541 cgatcatcga cgcgagcgcg atcgaggaca tgcgcacgcg cgccatcggg ctgtcggcga
      601 cgttgatgaa gtcgacgccg gcgtccttca ggaactgcgc gccctcgatc atcttcttcg
      661 gacgcgcgcc gcgcggcggg tcgacctcca cgctcaccac gaactccttc gccgcgagct
      721 ttcgcgcgag ggtggacctc gacgtgggat ccgtcttcgc ggtcgtctcc tcgatcacga
      781 tcgccgggcc cgcgcccggg ggtgaggggg cgaggggtgc cggggctccc gccgcggcgc
      841 ggagggcatc ccgcatcttc ctcgtgtgcg tcggcgtcgt cccgcagcat ccgcccacga
      901 ggcgcgcgcc cgccgccgcg aatcgctgcg cgtactcggc gagatactcg ggcgagctca
      961 gatagacgaa gcggccgtgc acgaaccggg gaagccctgc gttcggcatc acggagatcg
     1021 gaacctcggt gcggcgcttc agccgctcca cgatctcgag gagcttctgc gggcccacgc
     1081 tgcagttcgc gccgaggaga tcgaccttct tctccaggac cgcgtgcacc acgtcctcgg
     1141 gatacgagcc gtagagcgtc cggccgtcct cggtataggt catctggacc acgagcggca
     1201 cgtccccggc gacgctccgc acggcgtcga tcgcgacgag cgcctcggcc atgtcggtct
     1261 gcgtttcgag gatgatgaga tccacgccgc cttcgacgag cccttccgcc tgctcgcgat
     1321 acgcgtcgta cgcctcctcg cgggtgatcg tgccgagcgg cgcgacgggc ttgccgagcg
     1381 gccccatcga gcccgccacg aacaccgcct cgcccacttc ctcgcgcgcg ttccgcgcga
     1441 tctgcgcgcc ggcgcgattc acgcggcgcg cggtctcggt ggcgccgtgc gcggcgagcc
     1501 ggtagcgatt cccgccgaag gtgttggttt cgatgacctc ggcgcccgcc cggatgtact
     1561 cgcggtgcac cgcctggatc agcgacgcgt cggtcagatt gaggaggtcg aacgagcggt
     1621 cgaaggggat gcccttctcg tagagatagg tgcccatggc gccgtccgcc agcaccacgc
     1681 cctgctggac gcgcttgaga aacgggtggg tcatgggcgc gctccggcct ccgggcggag
     1741 gccctcgatc gctcgggtgt gttcttccat aagactcatc atcgtactat actgcgccgc
     1801 tcgccgttcg acctttcctg ggggaattcg cgcgtcatga acatcgtcgt cctcgtgaag
     1861 caggtgcccg acactgagac ccgcattcag gtcaaggacg gctcggtgga cctgagcggc
     1921 gtgaagtgga tcgtgaaccc gtacgacgag ttcgcgatcg aggaagcgct ccgcatccgg
     1981 gagaagctcg gccagggcaa ggtcacggcc gtgagcctcg gccccgatcg cgtgaaggaa
     2041 gcgatcaagt acgccctctc cctcggctcc gatgagggcg tctgggtgaa gggagacggc
     2101 gtcgcgctcg gcgatcccct cgccgtcgcc accgtgctcg ccgccgcggc caagaagatc
     2161 gggttcgatc tcgtcctcac cggcaagcag ggggtggact cggactggtc gcaggtcggc
     2221 ccgatggtgg ccgagctcct cgatcttccc cacgtctc
//
LOCUS       DASTKC010000159         7744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1780109, whole genome shotgun sequence.
ACCESSION   DASTKC010000159 DASTKC010000000
VERSION     DASTKC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780109"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VFR25_06250"
     CDS             complement(<1..1219)
                     /locus_tag="VFR25_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET9940683.1"
                     /translation="MNHPTGNGVQEREIKFRLPDDRDPAQVRQVIEAAGFRFEPTGTI
                     EHVDRYLDTDDWDLYRAGLSLRLRAEGSRLRLEAKSIASKPSEVLNRLEWAQDAPSAD
                     PPWEALDPGPVAGLLQPLLGLRVLERLQVRASVTNDRECFRWMRGESVIGLLTVDHVN
                     APPAAFREVELELDNGAEDALGLVRRAVEEQLGLTHEARTKLMAALEAAGVRLPERDE
                     RPFALHPADRLLDVAHKIFGRHLGRLLWNEPGSRLGVDPERVHDMRVAARRLRTALEV
                     VASGYPDSVREDFETDLRWVGRRLGRIRDLDVALEHVAKLEAEAAPFERPALHVFRQS
                     LEVRLARRRLRLVERLDSERFGVFLVKARTWVEAGPPVSASVPDAVMPAYAAAPKIIG
                     RWVDAMRVAYETAL"
     gene            complement(1230..1736)
                     /gene="sixA"
                     /locus_tag="VFR25_06255"
     CDS             complement(1230..1736)
                     /gene="sixA"
                     /locus_tag="VFR25_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HET9940684.1"
                     /translation="MDLYIVRHAIAVERGDPAYPWDDDRPLTPEGIHKFRLAARGLEK
                     LDVRPDHILSSPLIRARQTAEILRDAIAPQLEITYSPKLKPDSEYGDSLGAVAALGAK
                     SVAVVGHEPHLSGFASYLLCGPRQSASVVLRKGATALVRFPSEPAPGLGFLEWLLQPS
                     VLRRIAGS"
     gene            complement(1854..2906)
                     /locus_tag="VFR25_06260"
     CDS             complement(1854..2906)
                     /locus_tag="VFR25_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9940685.1"
                     /translation="MTEPERDPDIDLSVEAKRARDLLQRLEVPRPDGGFRARLKETFV
                     AGSFTEERGAPAPLEAEPADAPRRGGIGSMADDAPRRGGIGPIADDELDRTPHRPRAP
                     RTAPPPRPAAPRRPIFRFPKPTRPRFTTVLAAAAAIAIAVGILGNRGAQWRVVQVVGT
                     GLVQADGEPIPVERAELLAQRIHPGVELVLPETASLALAAGSDLAMEILPGTRLTIPP
                     APARWFLRKSELFARGGELRLTTGPTFQGAKLEVHTDDAMIQVKGTTLAVIMEPMGTC
                     VCVLEGEVMVGARGVSAGPEGSGGDAMMPITSGQRGYVFRDGRMPERADIRPTELVAL
                     AKFRSLQSELLERLAH"
     gene            complement(2903..3532)
                     /locus_tag="VFR25_06265"
     CDS             complement(2903..3532)
                     /locus_tag="VFR25_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9940686.1"
                     /translation="MTPQPERSAQVPAGYVPKNRDGGESPPLDLEAVRNRDPEALAAL
                     FERYFDRLYGMVYRMLGDRGATEEALQEVFLKVHRGAPSLDPSRDPGPWLVTIAANTV
                     RDLWRSSAYRFERASSSLDAEPGYAEELPSSAPDPERDTLTAERAEIVQRAIQSLRPS
                     FREIVVLREYEGLSYDQIAAITGENPTAVRKRYSRALDELGRFVTKAGL"
     gene            3674..4402
                     /locus_tag="VFR25_06270"
     CDS             3674..4402
                     /locus_tag="VFR25_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940687.1"
                     /translation="MGHFRTLAVPAALLFALVLPGCNDEDKILVENQPAPNNPPLIVS
                     YGPESPVENLAYFGPIGVDLWALTGDPDGLDDVSAVALHIDSIQLNQFIARPDTSTTG
                     CIQIGYAQTVDTAPILAVPATFPGIEFLAMRRTQGGLFTANGLGELYGAIDLEAASPV
                     LEDLGGYCGGNFHYVQGPMRAVPPGVPQVTTIVVTYMDVVYNGLKITVYDKVGASAVA
                     TVPAFHVVFVTPEERELITAEPAG"
     gene            4399..5127
                     /locus_tag="VFR25_06275"
     CDS             4399..5127
                     /locus_tag="VFR25_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940688.1"
                     /translation="MTARVRRMAPRLLLLAGLTLSLGCDDEDKVVVSSANPNDPPIVV
                     SYGPQVPQGGFHDVTGVGPYDGILFWVVAGDPDGLEDLSAVALSIQSVRLNRFIMRAD
                     TSSNSCFHFEYNDTVPTSQILATPAVLEGVAFEPLLSYPDYYGPGYYQGGHLPIPDLV
                     LLSPNVDPWPGGCGTSQEMVRGPMVILPPVVPTPTRAAVTYMDLDYQGVKLTVYDKVG
                     ATAVATYPDIHYVYVTPEEEQALP"
     gene            5216..6772
                     /locus_tag="VFR25_06280"
     CDS             5216..6772
                     /locus_tag="VFR25_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET9940689.1"
                     /translation="MPSTTKTSFPIRCAGMDVGSNAFRLVIAEFKSPDKYKVLERVRM
                     PVRLGASVFKEQRIDPATMDAALDAFREFRKKMEEYEVVLHRAVATSATREAKNRAAF
                     LERVHQETGLDLELIEGTEEARLVALGVKSKLSLSRGLSLILDVGGGSSEIAIIGNGE
                     ILVVESHDVGGVRLLRHANSESPEKTLGIVRQTLQSSRFPILDFFKRRHLSRFVGTGG
                     NIEAIAEIASPNGDARANGEHDAARVTLTRLSRTLAQLAKLDPKERAEEYDLRPDRAD
                     VIVPAGAIFEYVAKRVHAREIWVPFVGLVDGVLVDVALAASTEGKKELEVSQTRNAVL
                     ALLKKYEVDARHAHRVTALAVSLFDQLKAMHGLSKRDRLLLELAALLHEVGTFVGTSG
                     HHRHSLYLISKTPILGLSDTEQQVVANVARYHRKAPPDASHEAYAALSEKDQERVRSL
                     AAILRVADALDHDHKQRVSKVQAKQRGSDLKLRVSTRGDVSLDAWSLKDKGDLFENQF
                     GLKPVVVTRG"
     gene            6765..>7744
                     /locus_tag="VFR25_06285"
     CDS             6765..>7744
                     /locus_tag="VFR25_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940690.1"
                     /translation="MAEAHAIPSVPAAPPVVPAGPPVMSVIDVGASGIRMLIAELLPG
                     GEIRTLEELQRPLSLGRETFHTGTLSASSIQKAVVILRQYRAVMDTYHVTHSRAVATS
                     AVRGALNRDTFVDRVFLATGIEIEVIEGAQETLLTFAAVQRALEGHPELGTGDVLLFE
                     LGGGATEWALMRNGEVVASVTHDMGTVRMRELLRAAEGERRARTRMIQHNVKDMMNAV
                     RRALPFEKITQLIAIGSEARFAARHITGTNGSHAAGMAPHPVTPIAAKDLKKLADQIA
                     GMTPEQASATYEIPAYEAESLGPALLAYAELVRLSQADPLLVARVSMRDGL"
BASE COUNT         1234 a         2653 c         2644 g         1213 t
ORIGIN      
        1 gaagagcagt ctcgtaggcc acccgcatcg cgtccaccca ccgtccgatg atcttcggcg
       61 cggcggcgta ggcgggcatc accgcgtcgg gcacggaggc ggacaccggc gggccggctt
      121 cgacccaggt tctcgccttc acgaggaaga cgccgaatcg ctcggaatcg agccgctcga
      181 cgaggcggag ccggcgccgg gcgagacgca cctcgagcga ctgccggaac acatggagcg
      241 cggggcgctc gaacggggcc gcttccgctt ccagcttggc cacgtgctcg agagccacgt
      301 ccaggtcccg gatgcggccc agccggcggc cgacccatcg gaggtccgtc tcgaaatcct
      361 cgcgcacgga atccgggtag ccgctcgcga ccacctcgag cgccgttcgc aggcggcggg
      421 ccgccacgcg catgtcgtgc acgcgctcgg gatcgactcc gagccgcgag cccggctcgt
      481 tccagaggag ccgccccagg tggcgaccga agatcttgtg ggcgacgtcg aggagccggt
      541 ccgcgggatg gagcgcgaac ggtcgctcgt cccgctcagg cagccgcact cccgccgctt
      601 cgagcgcggc catgagcttc gtgcgtgctt cgtgcgtgag tccgagctgc tcctcgaccg
      661 cgcgccgcac gaggccgagc gcgtcctcgg cgccgttgtc gagctcgagc tccacctcgc
      721 ggaaggcggc cggcggcgcg ttcacgtgat ccacggtcag gagtccgatc accgattccc
      781 cgcgcatcca ccggaagcac tcgcggtcgt tcgtgacgct ggcgcggacc tgaagccgct
      841 cgagcacgcg gagtcccagg agtggctgga ggaggccggc gacggggcct ggatcgagcg
      901 cctcccacgg aggatcggcg gatggagcgt cctgcgccca ctcgagccgg ttgagcacct
      961 cggatggctt cgacgcgatc gatttggcct ccagccggag gcgcgagccc tcggcgcgaa
     1021 ggcgcagcga gagtcccgcg cgatagagat cccagtcgtc cgtgtcgaga tagcgatcga
     1081 cgtgctcgat cgtgccggtc ggctcgaatc ggaatccggc cgcttcgatc acctggcgca
     1141 cctgcgccgg gtcgcgatcg tcggggaggc ggaacttgat ctcgcgctct tgcacgccgt
     1201 ttccggtcgg atgattcatg ggcgggcgct caggatccgg cgatgcggcg gagcacgctg
     1261 ggttggagca gccattcgag gaagccgagt ccaggcgcgg gctcgctcgg gaagcgaacc
     1321 agcgcggtcg ctcccttgcg gagtacgacc gacgccgact ggcgcggacc gcagaggagg
     1381 tacgacgcga atccgctcag atgtggctcg tgccccacga ccgccacgga cttcgcgccg
     1441 agcgcggcca cggcgccgag cgaatcgcca tattcggagt cgggcttcag cttgggggag
     1501 taggtgatct cgagctgcgg ggcgatggcg tcgcggagga tctccgcggt ctgtcgcgcg
     1561 cggatcaaag gactcgacag gatgtgatcg ggccgcacgt cgagcttctc caggccgcgc
     1621 gccgccagcc ggaacttgtg aattccctcg ggggtcagag gacggtcgtc atcccacgga
     1681 taggcgggat cgcctcgctc gaccgcgatg gcatggcgca cgatgtagag atccactgag
     1741 actccctcag caagttgttg ttattaggcc gcttgacggg agtagagaag cgctggggcg
     1801 aatccattgt ctcactgccg gggatatgcg gtcaaccagg ccggagcggt ggttcagtgg
     1861 gccaagcgct cgagaagctc ggactggagc gagcggaatt tcgcgagcgc aaccagctcc
     1921 gtgggccgga tgtcggcccg ctcgggcatg cgcccgtcgc ggaagacata accccgctgt
     1981 cccgaggtga tcggcatcat ggcgtccccg ccggatcctt cgggaccggc cgacacgccc
     2041 ctcgcgccca ccatgacctc accttcgagc acgcagacgc acgttcccat cggctccatg
     2101 atgaccgcga gggtggttcc cttcacctgg atcatcgcgt cgtcggtgtg cacctcgagt
     2161 ttcgccccct ggaacgtggg gcccgtggtg aggcgcagct cccctccgcg cgcgaagagc
     2221 tccgacttgc gaaggaacca gcgtgcagga gcaggcggga tcgtgaggcg cgtgccggga
     2281 aggatctcca tggcgagatc gctgccggcg gcgagcgcga gcgatgcggt ctccggaaga
     2341 acgagctcga cgccggggtg gatgcgctgg gcgaggagct cggcgcgctc gaccgggatc
     2401 ggctcgccat cggcctgcac gagtccggtg cccaccacct ggaccacgcg ccactgcgcg
     2461 ccgcgattgc ccaggatgcc caccgcgatg gcgatggccg cagccgcggc gaggaccgtg
     2521 gtgaagcggg gccgcgtggg cttcgggaac cggaagatcg gtcgacgagg tgcagccggc
     2581 cgcggcggcg gcgcggtgcg cggcgcgcgg ggtcgatgcg gcgtgcggtc gagctcgtcg
     2641 tccgcgatcg gaccgatgcc gcctcgtctc ggagcgtcgt ccgccatgga accgatgcca
     2701 ccccgcctcg gtgcgtcggc gggctcggcc tccaagggcg ccggcgcacc ccgctcctcg
     2761 gtgaagcttc ccgcgacgaa cgtctccttg agccgcgcgc ggaacccgcc atcggggcgc
     2821 ggcacttcga gtcgctgcaa gaggtcgcgc gcgcgcttcg cctcgacgct caggtcgatg
     2881 tcgggatcgc gttccggctc ggtcatagtc ccgctttcgt cacgaagcgc cccagctcgt
     2941 ccagggcgcg ggaatatcgt ttgcgcacgg cagtcgggtt ctcgcccgtg atcgccgcga
     3001 tctggtcgta gctcaatcct tcgtactcgc gaaggaccac gatctcacgg aacgagggcc
     3061 tcaagctctg gatggcccgc tgcacgatct cggcacgctc ggccgtgagc gtgtcgcgct
     3121 cgggatcggg cgcgctcgac gggagctcct cggcataccc gggttccgcg tcgagcgagc
     3181 tcgacgcgcg ctcgaatcga tacgcggagg accgccagag atcccgcacc gtgttcgccg
     3241 cgatcgtgac gagccacggt cccggatccc gtgagggatc gagcgacggc gcccctcggt
     3301 ggaccttgag aaagacttcc tggagcgcct cctcggtggc ccctcggtct ccgagcatcc
     3361 ggtagaccat gccgtacagg cggtcgaagt agcgctcgaa gagcgccgcc agcgcctcgg
     3421 ggtcgcggtt gcggacagcc tcgaggtcca gcggggggct ctcgccacca tccctgttct
     3481 tgggtacgta gccggccgga acctgtgccg acctttctgg ctgcggggtc acgcggggga
     3541 cgccccaggg gcaagcattc gcccaggcta gcacacgcgt tgggcgaggt cccgcctggg
     3601 ctatccaggg ttgtaccgag ggagggttga gggtagggtt ttggcctcgc ctcatcggac
     3661 ctcaggggga cccatgggcc actttcgcac gcttgccgtt ccagccgcgc tcctgtttgc
     3721 cctcgtcctg cctggctgca atgacgagga caagatcctc gtcgagaacc agccggcgcc
     3781 caacaatccg ccgctcatcg tctcgtacgg ccccgagtct ccggtcgaga atctggccta
     3841 cttcggtccc atcggagtcg atctctgggc cctgaccgga gacccggacg gactggacga
     3901 cgtctcggcg gtcgctcttc acatcgactc catccagttg aaccagttca tcgctcgtcc
     3961 ggacacgtcg acaaccggct gcattcagat cggctacgcc cagaccgtgg acacggctcc
     4021 gatcctcgca gtgcccgcca cgtttccggg catcgagttc ctcgccatgc ggcgtacgca
     4081 aggtgggctc tttaccgcga atggcctcgg tgagctctac ggagcgatcg acctcgaggc
     4141 ggcctcaccg gtcctcgagg atctgggtgg gtattgcggt ggcaactttc actacgtcca
     4201 agggcccatg agggctgtgc cgccgggagt tccccaggtg acgaccatcg tcgtgacgta
     4261 catggatgtc gtgtataacg gcctgaagat cacggtctac gacaaggtcg gagcctcggc
     4321 cgtagcgacg gttccggcat tccatgtcgt cttcgtcacc ccggaggagc gcgaattgat
     4381 cacggcggag cccgccggat gaccgcgcgc gtccggcgca tggcgccgcg ccttctcctg
     4441 ctcgccggcc tcaccctctc cctcggatgc gacgacgagg acaaggtcgt ggtgagcagt
     4501 gcgaacccga acgatccccc catcgtcgtg tcctacggtc cgcaggtccc gcagggtggg
     4561 ttccacgatg tgaccggggt cggcccctac gacggcatcc ttttctgggt cgtggcaggc
     4621 gacccggacg gtctcgagga cctgtcggct gtcgcgctca gcatccagtc cgttcggtta
     4681 aatcggttca tcatgcgggc ggacacttca tcgaattcgt gttttcactt cgagtacaac
     4741 gataccgtgc ccacgagcca gatcctcgcg acgccggccg tgctcgaggg cgtcgcgttt
     4801 gagcctctcc tcagctaccc cgactactac gggcccggct actaccaggg tgggcatctt
     4861 ccgatcccgg atctggtcct gctctctccc aacgtggacc catggcctgg tggctgcggc
     4921 accagccaag agatggtgcg gggacccatg gtgatcctcc cgcccgtcgt tcccaccccc
     4981 acgcgcgcgg cggtgacgta catggatttg gactaccagg gtgtgaagct caccgtgtac
     5041 gacaaggtcg gtgccacggc tgtcgccacg tatcccgaca tccactacgt ctacgtgacg
     5101 ccggaagaag aacaagctct gccctgacct cgtcgaaggt gccgccgtaa gaaaccggcg
     5161 aaccgcccct acccgccgcc ttgacgcccg ggcgccccac ccccaagatg aggcaatgcc
     5221 ttcgacgaca aagacttcct tccccattcg gtgcgccggc atggacgtcg gctcgaacgc
     5281 gttccggctt gtcatcgcgg agttcaagag ccccgacaag tacaaggttc tggagcgggt
     5341 ccggatgccg gtccgcctgg gagcgtccgt cttcaaggag cagcggatcg acccggcgac
     5401 catggacgcg gcgctcgacg cgttccggga gttccggaag aagatggagg agtacgaggt
     5461 cgtcctccac cgcgccgtcg ccacgagcgc gacccgcgag gcgaagaacc gggccgcctt
     5521 cctcgagcgc gtgcaccagg agacgggact cgacctcgag ctgatcgagg ggaccgagga
     5581 agcgcggctc gtcgcactcg gcgtgaagag caagctctcc ctgagccgcg gcctgtcgct
     5641 catcctcgac gtgggcggcg gctcgagcga gatcgcgatc atcgggaacg gcgagatcct
     5701 ggtcgtggaa tcgcacgacg tcggcggcgt gcggctactc cggcacgcca attcggaatc
     5761 gcccgagaag acgctcggca tcgtgcgcca gacgctccag tcctcgcgat tcccgattct
     5821 cgacttcttc aagcgccggc acctctcgcg cttcgtcggc acggggggga acatcgaagc
     5881 catcgccgag atcgcgtcgc ccaacggcga tgcccgcgcg aacggcgagc acgacgcggc
     5941 gcgggtcacc ttgacgcgcc tcagccgcac cctcgcgcaa ctcgccaagc tcgaccccaa
     6001 ggagcgcgcc gaggaatacg acctccgtcc cgaccgggcg gacgtgatcg tgcccgcggg
     6061 cgcgatcttc gagtacgtgg cgaagcgggt gcacgcgcgg gagatctggg tgcccttcgt
     6121 gggtctggtc gacggcgtgc tcgtggacgt ggcgctcgcg gcgagcacgg aagggaagaa
     6181 ggagctcgag gtctcgcaga cgcggaacgc cgtgctcgct ctcctcaaga agtacgaggt
     6241 ggacgcgcgc cacgcgcacc gcgtcaccgc gctcgcggtc tcgctcttcg atcagctcaa
     6301 agcgatgcac ggtctcagca agcgggaccg gctcctcctc gagctggcgg cgctccttca
     6361 cgaggtgggg accttcgtcg gcacctcggg gcaccaccgc cactcgctct atctcatctc
     6421 gaagaccccg atcctcggac tcagcgacac cgagcagcag gtggtcgcga atgtggcccg
     6481 ctaccaccgg aaggcgccgc ccgacgcgag ccacgaggct tacgccgctc tgagtgagaa
     6541 ggatcaggag cgggtccggt cgctcgcggc catccttcgc gtggcggacg cgctcgacca
     6601 cgaccacaag cagcgcgtga gcaaggtgca ggcgaagcag cgcggctcgg atctgaagct
     6661 ccgggtgagc acccgggggg acgtgagcct cgacgcctgg tccctgaagg acaaggggga
     6721 tctcttcgag aaccagttcg gcttgaagcc ggtggtggtg acccgtggct gaagcgcacg
     6781 cgatcccatc cgtcccggcg gcgcccccgg tcgtgcccgc gggacctccg gtgatgtcgg
     6841 tcatcgacgt cggcgcgagc gggatccgca tgctcatcgc cgagctcctt ccgggcggtg
     6901 agatccggac gctcgaggag ctccagcgcc cgctctccct gggacgcgag accttccaca
     6961 ccggcacgct ctcggcgtcg agcatccaga aggcggtcgt gatcctgcgc cagtaccgcg
     7021 cggtgatgga cacctatcac gtgacccact cgcgcgcggt cgcgacgagc gccgttcgag
     7081 gagcgctcaa ccgggacacc ttcgtcgacc gcgtgttcct cgcgaccggg atcgagatcg
     7141 aggtcatcga gggggcgcag gaaaccctcc tcacgttcgc agcggtccag cgcgcgctcg
     7201 agggccaccc agagctcgga acgggggacg tgctgctgtt cgagctgggc gggggagcca
     7261 ccgagtgggc gctcatgcga aacggcgaag tcgtggcgtc ggtgacgcac gacatgggca
     7321 ccgtccgcat gcgggagctc ctgcgcgccg ccgaggggga gcgtcgggcg cgcacccgga
     7381 tgatccagca caacgtgaag gacatgatga acgccgtgcg gcgcgcgctt ccgttcgaga
     7441 agatcacgca gctcatcgcg atcgggtccg aggcgcgctt cgcggcgcgc cacatcacgg
     7501 ggacgaacgg ctcccacgcc gccggcatgg cgccgcatcc cgtcaccccg atcgccgcga
     7561 aagacctcaa gaagctggcc gaccagatcg ccggcatgac tccggaacag gcgtccgcca
     7621 cctacgagat cccggcgtac gaggccgagt cgctcggacc cgcgctcctc gcctacgcgg
     7681 agctggtgcg cctctcccag gccgatccgc tcctcgtcgc gcgcgtgagc atgcgcgacg
     7741 ggct
//
LOCUS       DASTKC010000160         8170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1781363, whole genome shotgun sequence.
ACCESSION   DASTKC010000160 DASTKC010000000
VERSION     DASTKC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781363"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFR25_06290"
     CDS             complement(<1..671)
                     /locus_tag="VFR25_06290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940691.1"
                     /translation="MTELPAGYLLRRPRDEDAQALVDLMVAIDIEEQGAPDTEIEDLQ
                     ADWRQPRFDKAKDAWTVTAPDRSLAGYGWVWDRVPHVDLQADLYVHPEHRGIGIEQVL
                     LDRIEERGREHVPDAPADAQVTVAVFAMAGTPAAEILERRGYERTRTFYRMTIDLVDG
                     YPEPTTPPGIEIRRFRPGVDDREVHATIEDSFAEHFRFHAESHEDWVTRRMEHPSFDP
                     SLWLVA"
     gene            complement(668..1756)
                     /locus_tag="VFR25_06295"
     CDS             complement(668..1756)
                     /locus_tag="VFR25_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HET9940692.1"
                     /translation="MPATSTQAAPRSAPPGGAAAILPGLALAVLVALAARLVHQSLPK
                     SVGTILGEVLFAVVIGLIVGNAVKLPDAMRPGLKFAFHAVLRTAIVLLGATFSFRQVL
                     QIGGKAVLLVVILMTLALLAAHLLGRAAKIPGRLATLIGVGTAVCGNSAIAATAPVIR
                     ARDDEVSFAIATNTVFGTLAVLLYPLIGKLVHMGDAAYGTWAGTAVNDTSQVVAAGFA
                     YGDLAGRVATAVKLTRNALMGGVILAIGIAYARSGAEVAAGSGAWGRIKQSFPIFVVG
                     FLLMALLNTLGFFVWLSAQVHVDVPRVLLETSRALILVALAAVGLSTRLSSMRETGLK
                     PFLIGLGVAALTSGASLLLIRILGPASL"
     gene            1833..2990
                     /locus_tag="VFR25_06300"
     CDS             1833..2990
                     /locus_tag="VFR25_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9940693.1"
                     /translation="MLQPRDIPEISPEDLVRTIESGGRIRILDIRAPQALAGGRIDLG
                     TPEQFLNMRGSEVVSLGEGIRSTLDPDTPLAVVCGHGNSSKEMALYLTGLGYEARSMI
                     GGMAAWGDALVPRDLTPPPGFDHLIQFDRISKGALGYLLAANGEAMIVDPPRKPQPFF
                     DVARDLGVKVTAVADTHAHADYISGGPLIAKRMGLPYYLHPKDAILPYDGSAATITFT
                     AIEDGQTLSLGGREIVVEHTPGHTEGSVCYRMGDDAVLTGDLIFIRSVGRPDLGGKLD
                     EWTPILWKSVSRSLKAWSPGIRVMPAHYAADAERESDRSVARTLARVREGNEPLKLSS
                     SEADFVAWVKKKVGSSPDAYRKIKAINLGLLKVWDIEAQELESGKNECALG"
     gene            complement(3015..3311)
                     /locus_tag="VFR25_06305"
     CDS             complement(3015..3311)
                     /locus_tag="VFR25_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940694.1"
                     /translation="MTQTQAVRKIKRSHWRSLDRLVRKAYLEGFDAGVARSHGAGRRG
                     RTIRADATVEGLVRRIERHFGLDRYDFEVRVVHPGSGRRVPSRDHLEKHLSTEQ"
     gene            3577..4488
                     /locus_tag="VFR25_06310"
     CDS             3577..4488
                     /locus_tag="VFR25_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET9940695.1"
                     /translation="MKLVACPQCHAQYDVSQSSGPTITCRCGATLKAEAPAARDATVT
                     RCSACGALVGDKDRACTYCGALIVRRPPPSGPVCPECYAQNPEGAKFCVSCATHFLPQ
                     PVRESVVNLACPVCPDSALVARDLGGIWVDECPNCLGLWAPGDVLDRLVDRVRERAAA
                     EGIPPATPERKRRSVWQSGIAYRKCPECGTGMQRRNFAHKSGVVVDWCGSHGTWLDAH
                     ELEDIAAFVLEGGLRTAPTVTIEEHTWTLPSDPKRVAALAAAERILAEEKARVEVTTH
                     RWSARRDLPQGFRGIGDLLTFLLTRRV"
     gene            4521..5603
                     /locus_tag="VFR25_06315"
     CDS             4521..5603
                     /locus_tag="VFR25_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9940696.1"
                     /translation="MGFVEKLRGEVVDIVEWIDDSRNTVVWRFPRYHNQIKNGARLVV
                     RPGQAAVFVEQGRIADVFGPGTYELVTANLPVLSTIRGWKHGFDSPFKAEVYFVATRQ
                     ITELKWGTPNPVILRDPTLGPLRVRAFGSYTLRATHPEALLKELVGTDGSFQTDEIDV
                     LLRSIITACFTDLIASAPIALADLSSNAMRLSEELRKKVLAKIDDEYGLDVPQLYIVN
                     ISVPDRVEQALDARSSMELIGDIDRYQRYQVGAATPIAAANPSGGVAGAGVGLGMGMA
                     YAGAVTRAPLQVVPAAPAGAVWHVANAGRITGPFTVEQLRNAVAAGQLQATSTVWTAG
                     MDAWTPMGSVPALAIYFAPPPPPPAA"
     gene            complement(5608..6603)
                     /locus_tag="VFR25_06320"
     CDS             complement(5608..6603)
                     /locus_tag="VFR25_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940697.1"
                     /translation="MMARRELLVAVLAALLAAGCSRTPSSEERRGWDLEVARLQAEQD
                     SLRARAAVLIAADPRIQGLPEGEVVVAVPTAFIRQVIDRLFQDVVNRVTLRLSGIKAH
                     VAKKVKKVVTVGEFTVDVNIDRVIGKIGPKPPRIAFGGDSISLKLPIAIKEGRGEATI
                     HFVWDGKRVAGLTCGDLDITQKVSGNVIPAGYLVTGRIDLSIKGRRVVAALVFPETKL
                     RIKVKPSKESWAAIDSILDEQKHVCGWVLDKVNVPGLLENLTETKGFNVKLPVDKLKP
                     VILPAGIRDSVRVGERSFAIDAQTKSLRIDPDAIWYSAAVKLEGVRPESPPEPRP"
     gene            complement(6600..7385)
                     /locus_tag="VFR25_06325"
     CDS             complement(6600..7385)
                     /locus_tag="VFR25_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940698.1"
                     /translation="MTRTWAVGVVTLAALLSGCAPSDDELDYLAQKALLERQNQGIRE
                     LIQEEERGSLVPRDRFLIGIDEEVVGDLFRSQLPIEKPVGKRIAVHLENATVSLRDKF
                     GIIVIEGYANRLKTPDRRVRIRIHGGLGAVSVDPNTDLLHVKIAIDRIEILEAGLLEG
                     VLGRGGKALLARKGLPRIQEAIPELEVPVVLGRSIQVPAIQEEGFQLDSLVVPLDLSV
                     ERVIAAGGKLWVTLDAKVGKVTGGEEGIGVNVQKKPKKKAGAS"
     gene            7587..>8170
                     /locus_tag="VFR25_06330"
     CDS             7587..>8170
                     /locus_tag="VFR25_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940699.1"
                     /translation="MKLAGMRIAGALIALLLPIAAHAQTGKHIAIGAAVSSHDYVDSR
                     VHQGVGIMLLYRVAPTGMEANGLSWGPSATVGFSRADVRDDATGGDVTLGKLQSIPAM
                     AGFGPHYRHGRWWTGLSVTAGASFNDFSVDEAARQAYEDRKGVELESVDAKTSFAAQP
                     GVSVWYDVGSRLGLYGGVAYFYSRPKASITAGGVT"
BASE COUNT         1313 a         2781 c         2775 g         1301 t
ORIGIN      
        1 gccacgagcc agagcgacgg atcgaagctc ggatgttcca tgcggcgcgt gacccagtcc
       61 tcgtgcgatt ccgcatggaa gcggaagtgc tccgcgaacg aatcctcgat ggtcgcgtgc
      121 acctcgcggt cgtccacacc ggggcggaac ctacggatct cgatccccgg cggcgttgtc
      181 ggctcggggt atccgtccac gagatcgatc gtcatgcgat agaaggtgcg cgttcgctcg
      241 tacccgcgcc gctcgaggat ctccgcggca ggggtgcccg ccatcgcgaa cacggccacg
      301 gtgacctgcg cgtcggcggg cgcgtccggc acgtgctccc ggccgcgctc ctcgattcgg
      361 tcgagcagca cctgctcgat gccgattcca cggtgctcgg ggtgcacgta gagatcggcc
      421 tggagatcca cgtgcggcac gcgatcccac acccagccgt agcccgcgag cgagcggtcg
      481 ggggccgtga cggtccaagc gtccttcgcc ttgtcgaagc gcggctgcct ccagtcggcc
      541 tggaggtcct cgatctccgt gtcgggagcg ccctgctcct cgatgtcgat cgcgaccatg
      601 agatcgacca gcgcctgggc gtcctcgtcc cggggacgcc ggaggagata gccggccggg
      661 agctcggtca tagcgaggcg ggtccgagga tccgaatcag gaggaggctc gcccccgacg
      721 tgagcgcggc gacgccgagc ccgatcagga agggcttgag tccggtctcg cgcatcgacg
      781 agagccgggt cgagagtccc accgccgcca gtgcgaccag gatcagcgcg cgcgatgtct
      841 cgagcagaac gcgcggcacg tcgacgtgaa cctgagccga gagccagacg aagaatccga
      901 gcgtgttgag gagcgccatg aggaggaacc cgacgacgaa gatcgggaac gattgcttga
      961 tgcgccccca cgcgccgcta cccgcggcga cctcggcgcc cgaccgcgcg tacgcgattc
     1021 cgatcgcgag gatcacacct cccatgagcg cgttccgcgt cagcttgacc gctgtcgcga
     1081 cgcgcccggc caggtcgccg tacgcgaagc cggccgcgac gacctgagac gtgtcgttca
     1141 ccgcggtgcc cgcccacgtg ccgtaggccg cgtcccccat gtgcacgagc ttcccgatca
     1201 gcgggtagag gagcaccgcc agcgttccga acacggtgtt ggtcgcgatc gcgaacgaga
     1261 cctcgtcgtc gcgcgcgcga atcacgggag cggtcgccgc gattgccgag ttgccgcaga
     1321 cggcggtgcc gacgccgatc agggtcgcga gccgtcccgg gatcttcgcg gcgcggccga
     1381 gcaggtgagc cgcgaggagc gccaaggtca tgagaatgac gacgaggagc acggccttgc
     1441 cgccgatctg gagcacctgg cggaacgaga acgtcgcccc aaggaggacg atcgcggtcc
     1501 ggagcacggc gtggaacgcg aacttgagcc ccggccgcat cgcgtcgggt agcttcaccg
     1561 cgttcccgac gatgaggccg atcacgacgg cgaagaggac ctcgccgagg atcgtcccca
     1621 cgctcttcgg cagcgactga tggacgagcc gggccgcgag cgcgacgaga acggccagcg
     1681 caaggccggg caggatggcg gctgcaccgc ccggaggggc ggatcgaggc gcggcttgcg
     1741 tgcttgtggc gggcatccgc gtatcttgca cggggctcga tgccgcgtca aacgaggttg
     1801 acgaaggcag cccgcctgcg cacacttccg ccatgctcca acctcgggat atccccgaaa
     1861 tcagccccga agacctcgtc cggaccatcg agtccggagg gaggatccgg atcctcgaca
     1921 tccgggcgcc ccaggcgctc gcggggggac ggatcgatct gggcactccc gagcaattcc
     1981 tgaacatgcg cggatcagag gtcgtgtccc tcggggaggg catccgctcg acgctcgatc
     2041 cggacacgcc cctcgcggtg gtttgcggac acgggaactc gagcaaggag atggcgctct
     2101 acctcaccgg gctgggctat gaagcccgct cgatgatcgg cggcatggcc gcatggggcg
     2161 acgctctcgt cccgcgcgat ctcacgccac cgcccggatt cgatcacctg atccagttcg
     2221 accggatctc gaagggcgcg ctcggctatc tcctcgccgc gaacggcgaa gcgatgatcg
     2281 tggatccgcc tcgcaagccg cagcccttct tcgacgtcgc gcgcgatctc ggggtcaagg
     2341 tcacggccgt ggcggacacg cacgcgcacg ccgactacat cagcggcggc cccctgatcg
     2401 cgaagcggat gggccttccc tattacctcc acccgaagga tgccatcctt ccctatgacg
     2461 gctccgccgc gacgatcacg ttcaccgcga tcgaggacgg ccagacgctc tcgctgggag
     2521 gccgcgagat cgtggtcgag cacacgcccg ggcataccga gggaagcgtc tgctatcgca
     2581 tgggcgatga cgccgtgctc accggggatc tgatcttcat tcgctcggtg ggacgccccg
     2641 acctgggcgg gaagctggac gagtggacgc cgatcctctg gaagagcgtg tcccgctcgc
     2701 tcaaggcgtg gtcgcccgga attcgcgtca tgccggcgca ctatgccgcc gacgccgagc
     2761 gcgagtccga tcggtccgtg gcccgcacgc tcgcgcgcgt gcgtgaaggc aacgagcctc
     2821 tcaagctcag ctcgtcggaa gcggacttcg tggcgtgggt gaagaagaag gtgggatcga
     2881 gccccgatgc ctaccggaag atcaaggcga tcaacctggg ccttctcaag gtctgggaca
     2941 tcgaggccca ggagctcgag tccggcaaga acgagtgcgc gctcgggtag agccgcccgc
     3001 gcaccctccc gccgttactg ctccgtgctg aggtgcttct cgaggtggtc ccgcgaaggg
     3061 acccggcgcc ccgaccccgg gtgcacgacg cgcacctcga aatcgtagcg atccagcccg
     3121 aagtgccgtt cgatccggcg cacgagacct tcgacggtcg cgtccgcgcg gatggtcctg
     3181 cccctccggc ccgcaccgtg ggagcgcgcg acacccgcgt cgaacccctc gaggtaggcc
     3241 ttccggacga gccggtccag gctcctccag tggctccgct tgatctttcg tacagcctgc
     3301 gtctgcgtca ttccttctcc tcctcttccc gggccctcta agtagctatt ctctaaagaa
     3361 atagcggccc acggggtctg tctgccgact gtttggacga ccgccgctcg ggaacgatgc
     3421 gggggcctcc gtagtcggca tgcaactgcc tgcaattata gcacttgtct gtcgctccga
     3481 gaaccgcccc gtggaggtct ttgggtcaag atccacaacc ctcctgccga tccattcggg
     3541 ggctcatccc tccgtcccgg accaggagca cgcgccgtga agctcgtcgc ctgtccccag
     3601 tgccacgcgc agtacgacgt cagccagtcg agcgggccga cgatcacatg ccgctgcggc
     3661 gcgaccctca aggccgaggc ccctgcggcc cgggacgcga ccgtgacgcg atgctcggct
     3721 tgcggcgccc tggtcggcga caaggaccgg gcgtgcacgt actgcggcgc tctcatcgtg
     3781 cgccgccctc caccgtcggg cccggtctgc cccgagtgct acgcacagaa tcccgaaggg
     3841 gcgaaattct gcgtctcgtg cgcgacccac ttcctgcctc agcccgtgcg ggagagcgtc
     3901 gtgaacctgg cctgcccggt ctgccccgac tcggcgctcg ttgcgcgcga tctcggcggg
     3961 atctgggtcg atgagtgccc caattgcctc ggcctctggg ctcccggcga cgtgctcgac
     4021 cgcctggtcg atcgcgtgcg cgagcgcgcg gcggccgaag gcatcccgcc ggccaccccc
     4081 gagaggaagc ggcgatcggt ctggcagtcg ggtatcgcgt atcgaaagtg ccccgagtgc
     4141 gggaccggga tgcagcgccg gaactttgcc cacaagtcgg gcgtcgtcgt cgactggtgc
     4201 gggagccacg ggacgtggct cgacgcgcac gagctcgagg acatcgcggc gttcgtgctc
     4261 gagggcggcc tcaggacggc gccgaccgtg accatcgaag agcacacctg gaccctgccc
     4321 tccgatccca agcgcgtggc cgcgctcgcg gcggcggagc ggatcctcgc ggaggagaag
     4381 gcacgcgtgg aagtcacgac ccatcggtgg agcgcgcgcc gcgatcttcc tcagggcttt
     4441 cgcggaatcg gagacctgct gacgttcctg ctcacacgcc gcgtctgacg cgcggctcag
     4501 gctgaatcag ggaggagccc atgggattcg tggagaagct tcgcggcgag gtggtcgaca
     4561 tcgtcgagtg gatcgacgac tcgcggaaca cggtcgtttg gagatttccc cgctaccaca
     4621 accagatcaa gaacggcgcc cggctcgtcg tgcgtcccgg gcaggcggcc gtgttcgtgg
     4681 agcagggacg gatcgccgac gtgttcgggc cgggcaccta cgagctcgtg accgcgaacc
     4741 ttccggttct ttccacgatc cgcggctgga agcatggctt cgacagcccg ttcaaggcgg
     4801 aggtctactt cgtcgcgacg cgccagatca cggagctcaa atgggggacg ccgaatccgg
     4861 tgatcctgcg cgacccgacg ctcgggccgc tgcgcgtgcg cgcgttcggc agctacacgc
     4921 ttcgcgccac gcaccccgaa gcgctcctca aggagctggt gggcaccgac ggctccttcc
     4981 agaccgacga gatcgacgtg ctcctccgct cgatcatcac cgcctgtttc accgatctga
     5041 tcgcctcggc gccgatcgcg ctcgcggatc tctcgtcgaa cgcgatgcgc ctctccgagg
     5101 agctgcgaaa gaaggtcctc gccaagatcg acgacgagta cgggctcgac gtgccgcagc
     5161 tctacatcgt caacatctcg gtgccggatc gggtcgagca ggcgctcgac gcacgctcca
     5221 gcatggagct gatcggcgac atcgatcgct accagcgcta tcaggtcggc gccgccacgc
     5281 cgatcgccgc cgccaatccc tccggcggcg tcgcgggagc gggcgtggga ctcggcatgg
     5341 gcatggcgta cgcgggcgcg gtcacccgcg cgccgctcca ggtggtgccc gcggcgcccg
     5401 cgggcgcggt atggcacgtg gcgaacgcgg ggcgcatcac gggtccgttc acggtcgagc
     5461 agctgcgaaa cgccgtcgcg gcggggcagc tccaggcgac gagcaccgtc tggaccgccg
     5521 gcatggatgc gtggacaccg atgggctcgg taccggcgct ggcgatctac ttcgctccgc
     5581 cgcctccgcc gccggccgcg taagcggcta gggtcggggc tccggcgggg actccggccg
     5641 caccccctcc agcttcacgg cggcgctgta ccagatcgcg tccgggtcga tgcggagcga
     5701 cttcgtctgg gcgtcgatcg cgaacgaccg ctcacccacc cggaccgagt ctcgaatgcc
     5761 cgcgggcagg atcacgggct tcagcttgtc gaccggaagc ttcacgttga agcccttcgt
     5821 ctcggtgagg ttctcgagca ggcctggcac gttcaccttg tcgagcaccc agccgcacac
     5881 gtgcttctgc tcgtccagga tcgaatcgat tgcggcccag gattccttcg atggcttcac
     5941 cttgatgcga agcttcgtct ccgggaacac gagtgccgcc acgacgcggc gccccttgat
     6001 gctgaggtcg atccggcccg tcacgagata gccggccgga atcacgttgc ccgacacctt
     6061 ctgggtgatg tcgaggtcgc cacaggtcag accggccacg cgcttcccgt cccagacgaa
     6121 gtggatcgtc gcctccccgc ggccttcctt gatcgcgatg ggaagcttga gcgagatgct
     6181 gtcgccgccg aacgcgatcc ggggcggctt gggcccgatc ttgccgatga cccggtcgat
     6241 gttcacgtcc acggtgaact cgcccaccgt caccaccttc ttcaccttct tcgcgacgtg
     6301 cgccttgatc ccactcaggc ggagcgtgac gcggttcaca acgtcctgga agagtctgtc
     6361 gatcacctgc cggatgaatg ccgtgggcac cgcgaccacg acctccccct cgggcaatcc
     6421 ctgaatgcgt gggtcggctg cgatcagcac cgcggcccgc gcgcgaagcg agtcctgctc
     6481 cgcctggagc cgcgcgacct cgagatccca gcctctccgc tcctcggacg agggcgtgcg
     6541 ggagcagccc gcggcgagaa gcgcggccag cacggcgacg aggagctcgc gccgggccat
     6601 catgaagcgc ccgccttctt cttcggcttc ttctgcacat tgacgccgat tccctcctcg
     6661 ccgccggtga ccttgccgac cttggcgtcg agggtgaccc acagcttgcc gccggcggcg
     6721 atgacgcgct ccaccgagag atcgagcggg acgacgaggg aatcgagctg gaatccttct
     6781 tcctggatgg cgggcacctg gatggatcga ccgagcacca cggggacttc cagctcggga
     6841 atcgcttcct ggatgcgcgg gagtcccttg cgcgcgagga gcgccttgcc gccgcggccg
     6901 agaacgcctt cgaggagccc ggcctcgagg atctcgatcc ggtcgatggc gatcttcacg
     6961 tggaggaggt cggtgttggg atcgacggac acggcgccga ggccgccgtg gatgcggatg
     7021 cggaccctgc gatcgggcgt cttgaggcgg ttcgcgtagc cctcgatcac gatgatgccg
     7081 aacttgtctc gaagggagac cgtcgcgttc tcgaggtgga cggcgatgcg cttgccgacc
     7141 ggcttttcga tcgggagctg cgatcggaag agatcgccga ccacctcttc gtcgatcccg
     7201 atgagaaagc ggtcgcgcgg cacgagggag ccgcgctcct cctcctggat cagctcccgg
     7261 atgccctggt tctgtcgctc gaggagcgcc ttctgcgcaa ggtagtcgag ctcatcgtcg
     7321 gagggggcgc atccggagag gagcgccgcg agggtcacga ccccaacagc ccaggtccgc
     7381 gtcacgagcc ggaggatagc cgcaaggccc ctacttgttt gaagaatctg cagggctctg
     7441 cccttggaga gggctcgctg accacaccgt aaggtcgccc gtttcaggcg gatggaagcc
     7501 ctgtcttgga tgccgcgtcg agtggcctcc gatttgcgta aagggggagc cggatgatcg
     7561 tgcgtccctc atcggaggtt tgaatcatga agctcgcagg aatgcgaatc gcgggagcgc
     7621 tcatcgcgct gctcttgccc atagcggcgc acgcgcagac tgggaaacac atcgcaatcg
     7681 gcgcggccgt ttcgagtcac gactacgtgg actcgcgcgt ccaccagggc gtcgggatca
     7741 tgctcctcta ccgggtggca ccgacgggaa tggaagcgaa cggattgtcc tggggaccga
     7801 gcgcgaccgt cgggttttcc cgcgccgacg tgcgggacga tgccaccggt ggggacgtca
     7861 cgctcggcaa gctccagtcg attccggcca tggccggctt cgggccgcac taccggcacg
     7921 ggcggtggtg gaccggtctc tcggtgacgg cgggcgcgtc gttcaacgac ttcagcgtcg
     7981 acgaggcggc gaggcaggcg tacgaggacc gtaagggagt cgagctggaa tcggtggacg
     8041 ccaaaacctc gttcgcggct cagcctgggg tgagcgtctg gtacgacgtc ggatcgaggc
     8101 tcgggctcta cggaggcgtg gcgtacttct acagccgccc caaggcgtcg atcacggctg
     8161 gaggcgtgac
//
LOCUS       DASTKC010000161        15670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_599903, whole genome shotgun sequence.
ACCESSION   DASTKC010000161 DASTKC010000000
VERSION     DASTKC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599903"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFR25_06335"
     CDS             complement(<1..294)
                     /locus_tag="VFR25_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HET9940700.1"
                     /translation="MTQIPVEAPPAASELVPLSDAYRVCADIVRRHEENFPVVSRFIA
                     SPLRPALAAVYAFARLADDVADAAAPSAERLDGLDRIEAALLEALDGDPKGPVL"
     gene            complement(1251..2354)
                     /locus_tag="VFR25_06340"
     CDS             complement(1251..2354)
                     /locus_tag="VFR25_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9940701.1"
                     /translation="MDFRYSRWDERLTQNLNFQRNLLSLYHRLLLMTDGNVEQALRAL
                     EELGERFGFFNEKYKIEDFKNHLKEKDAIEEVDGRPVLTRAGERMIRKDSLDRIFGNL
                     AKDSAGEHRVPRTGQGGEKITETRPYQFGDSVNDIDFLSSVRNSMRRQRGEWGDQGLG
                     LTEDDLEVFETEHLSSCATVLLIDVSHSMILYGEDRITPAKQAALALSELILTRYPKD
                     SLSVVLFGDDAWEVKIRDIAYATVGPFHTNTKAGLQLAQKILARQKHVNKQIFMITDG
                     KPSAIFENGRFYKNAFGLDPRIVNKTLDEAVACRRKRIPITTFMVTQDPYLVRFVERF
                     TQLNKGRAYFSDLEELGSYLFVDYAQNRKRRVQ"
     gene            complement(2365..3846)
                     /locus_tag="VFR25_06345"
     CDS             complement(2365..3846)
                     /locus_tag="VFR25_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9940702.1"
                     /translation="MNRPQTIKQLRDSGYQPRTVKQEMRANLIRAIESGEDRFPGIVG
                     YDKTALPQIENAILSGHDFILLGLRGQAKTRILRQLFRFLDEWMPAIEGCEINDDPLN
                     PLCPVCQRKVAMHGDEVAIRWIPREERYREKLATPDVTIADLIGDIDPIKAANQKLSY
                     SDPEVMHYGIIPRTNRGIFAINELPDLQPRIQVGLLNILEEKDIQIRGFPIRLPLDLQ
                     IVFSANPEDYTNRGNIITPLRDRIASQIMTHYPYTIEDAMKVTTQEAWVKRPDGIEIL
                     VPSYIQELVEHVALQARKSSYVDQSSGVSVRMTIALMENLVSNAERRAIKTGERRQAV
                     RICDLQNGISSITGKVELVYEGEQEGAVAVAKHLVGKAVKEVFTKYFPDAYKAKEKNE
                     GAASEYDPIFRWFAQGKKVEISDELPAREFHQRLRQVAGLEELPKRYLPVKDPAELPT
                     AMEFVLEALHQNSILAKERTDTAALAYTDMFSTMLGKPDQAED"
     gene            4005..4493
                     /locus_tag="VFR25_06350"
     CDS             4005..4493
                     /locus_tag="VFR25_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET9940703.1"
                     /translation="MTTELRNKVENGTLDATDLRILALLQDNAKMPQAEMAREVGLTA
                     PSVNERIKKLERSGYIRGYVTLLDERKLGQDVTAFVEVFIEHPKHEAGFIEAASTLDE
                     VLECHHITGEFSLLLKVRVADMGAFRRLLIEKLNTIEGVRQTRTLIVLATSKEQHRIK
                     LD"
     gene            4564..5919
                     /gene="lat"
                     /locus_tag="VFR25_06355"
     CDS             4564..5919
                     /gene="lat"
                     /locus_tag="VFR25_06355"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HET9940704.1"
                     /translation="MNPTMTGASVHVTPDQVHSTLAKHMLADGFDFVLDLDKSQGVWL
                     HDSRGNKTLMDFFSFFASNPVGMNHPGVKNPEFQARLLKAAVHNVTNSDVYTTEMAEF
                     VETFARHAMKDTFAHSFFVAGGTLGIENALKAAFDWKVRKNFARGYREERGTKVLHFE
                     QAFHGRSGYTLSLTNTTDPRKVAYFPKFDWPRVTNPKVEFPLNEDRLKDVKAREALAV
                     AQMERAFEQNPGDIAAIIIEPIQGEGGDNHFRPEFFKELRRLADQNDAMLVFDEVQAG
                     FGLTGKFWAFEHYGVTPDMVGFGKKSQICGFIATKRIDEVQDNVFHVSSRINSTWGGT
                     LVDMVRSQRYLEIMAEERLLENATARGLELLTGLEGLVKEFPEHVMNARGKGMMCAFD
                     LRDGALRDQVISNAYAEGVVVLGCGPTSIRFRPPLVITKDEIAQGLEKFRSSIKKAIG
                     R"
     gene            complement(6040..9732)
                     /locus_tag="VFR25_06360"
     CDS             complement(6040..9732)
                     /locus_tag="VFR25_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9940705.1"
                     /translation="MPPATGSNVSLYRSSMSYPEGIVRNNTELTGYPVCITSGDFNGD
                     GNPDFAVANLDSGFAGSVGSISILTHAGSQYLPPLPWSASAQPDFPALNATFFNYPVP
                     PNGKLHLPLNMVARDFNGDGRTDIAVARYASSAILWNTGQTPASGQQFVAKSSTYASE
                     AIGTADFDNDGVLEIVGLTSGAPSNLFARVFHTAADTLQLIGTTMLGPLAPRHILSFL
                     AIADVTGDGLPDVIAVDDNDPPDCAEAAMYVATGYNAGTFTTILGPFAPGSGVLDLAV
                     APLDGDAIPDVVTTTFCANAVRISTGHPGGTFDPGVSITLPHAPEKICLGDLDGDGDT
                     DIVTLASNSPYIAGTVCVVKGNGDGTFGPPVLYDGLPRSSAPDLLINFQLQYGEAILV
                     DVNRDGRLDLVATEMGTKHTIGVLLNDGSGGFRSGTRSGIAQRTYRQAIADFNRDGFP
                     DVASSGMSEVTIGFGNGTGKFTQGPSFTVTQPTTLVAGDINRDAKMDVLVQTGSSFRP
                     YLGNGDGTFTPHQILFGGLVYSRSIADITRDGNPDMVAVDGQNVKIYAQNQFGFWMLL
                     YQFQGPVQQPWGLAIADWNRDGLLDIALASDLGIHAYISTGIGTWTPNPVPIGSGRFK
                     DVCAGDFNRDGYVDLAGRLDTSGTSVVSGIQVFLNGPSGLQPAPSGSTYTTLEKRGFF
                     LDSWDADIDGIPDLIASGSTGIGDVSSFSSFDVLLGNGMGAFGLRTSYAMGTIDERTP
                     PVMTSGEYLAAGDVDRDGIPDLMAGAVVSPDSSQVYTALTSPPTYTASLHDAVFKTTL
                     SNPKDVAVGDLDRDGKLDVVTGTEGANPGVAVHRGGGGGTIGSPTTLAQSWYGTRVRL
                     ADLNRDGILDIVGASSVAGAKRIYTMLGMGNLTFGARVDYLMNVGSDIEVGDMNRDGI
                     PDVVVSTPDSVRVLTGTGTGTFTAFSGVALAAPIHEIDLADLNRDGVLDVLCANGVVS
                     VIYGNANGTLGTPVTTGVQSQTAQVVCSGDLNRDGYLDIVANQGNGYFVSFGDATFPF
                     GSSFISFLSVTAFDMEIAPMAADGRPYLVASSGLDRVEVASVGAFGAFTSAGIYPVSA
                     TPESEVLADMDRDGLLDVVSVGSAGSTVAINLRGTGTVTAVEPSEAPAPRVVQLHQNY
                     PNPFNPETTIRFTLPESRKIRLTVHDAQGRLVAVLANQQIAAGRHEVPWHGKNAEGHP
                     VASGVYFYRLATDDGAVQSKRMVLVK"
     gene            9926..12355
                     /locus_tag="VFR25_06365"
     CDS             9926..12355
                     /locus_tag="VFR25_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9940706.1"
                     /translation="MRRPLALLLAAIIAVPITTPRTSRSQESPDTTPPRAGQSQEVTT
                     APRDQALVLSGGGSRGIAHAGVIAGLEKKGYSPGIVVGTSMGAVIGALYASGMNADSV
                     WSVVDMQDWRQIFAPFPSPVGLDRSVHYPVLRLQGTGNSPELRGYLDDWQINRQLVRQ
                     LFRPSAAVRGDFDRLPRRFRSIAADLRSGHLVSIGYGDLARSVRASMAQAGVFAPTAW
                     DDRVLTDGGVSDYFPVAEARRLGARDVIGVDVLRPTAEIHSTEASAVMDRSLRLLVQH
                     ARADTLPPDILIAPDIDPTISPISYPTDPEPLFMDGYRATNSLPQGAGSSEPPGTLPA
                     LPDSLAEVRVLVDSPLRPLLKRAFDRFGKARFDADQILKYVDRLYATGLFTGVWPSVE
                     GAPAQPPGTPASSARSTVTPPAQVAPPTAPPVEEGREGFDGVDNDSTEVEMPAVGAPA
                     DTLSEPPSGESSASPPAASSAPPDSATLSALGAAPDTSSTPAAVSDAVPALLIRAETV
                     GALSVNGAVGYDNDRGGRGWANLRKLGAMGSHTVEFALEAMANGIEQSASMNVRVPTV
                     DRLPTAWSGGLYLAESQIRFLSVRPDGDDPDVKRGGGWIGAEVRKISPAIEATAIFTA
                     ENLDANVGPSGDSFGPMVRIGFIPDNLQLVGIPTSAEGEARTGDARYARVRARGSRVF
                     AFKKLTVAPLFDVAAVTQGTPRDVVPSPGMDQLVPALRWGERRGRAQAIAGVDAAFPL
                     FTKIRVRLRGGGSADQLLPDGSDFSSATLWFGGASVSALWWTPFGRIEGGLEANTLGD
                     RRAVVSVGQDF"
     gene            complement(12352..12957)
                     /locus_tag="VFR25_06370"
     CDS             complement(12352..12957)
                     /locus_tag="VFR25_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9940707.1"
                     /translation="MSRDNHHLLRTQDLLREAQQGRRDALETLLARYRPRLERWASGR
                     LPRNARSLLDTGDLVQETLLRALETIGSIGARHPGSFEAYVRHAVLNRIRDQIRWARR
                     RGDGNLESLVDPTPSPLEEAIGSDVVRRYEEGMSLLSEDERQILHLRIELDMGYEDIA
                     AMTDRPSADAARMAIQRSLHKLAIAMRKVSSPGHSRPGPAA"
     gene            complement(12954..15371)
                     /locus_tag="VFR25_06375"
     CDS             complement(12954..15371)
                     /locus_tag="VFR25_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9940708.1"
                     /translation="MEPGDNVDRLTGSVSDGKPVDWDHVELDAELDSDTVRMMRDVAR
                     IAEYNRALQRAATPSSSPPSPQDPPLERWRDLTLMEPLGAGARGEVWRAWDSTLRRQV
                     ALKFLQTAPGESASAELLSEARALARIRHPGVVTVFGIAEDQGRFGMWMECIPGTSLA
                     REIERVGALRPSQVALIGAQLCSALEALDAAGLVHRDIKPANILLEREDRAVLTDFGL
                     GWRPALDDEETPRSSGTPLFMAPELLAGERPTPQSDLYALGVTLWWALAGRPPFKART
                     LAELREEAPRGPSPSLRSIRPEVSKDFEDAILAAMAPSRSDRVRTAAELGGRFQTIAR
                     GLGTAAHAGTLSIAVLPFVNRSPGTEDEYFSDGLADELIGMLGKIRGLRVAARTSAFS
                     FRGRQATVEEIGRALHVDTVLDGSIRRSGDRVRISVQLVKVSEGLHLWSETYDRTLDD
                     IFAVQDDIAQSVVKELRAALLGDTGPDVNAEIARAARGRATDPAAHRLYLLGRYFITR
                     LNREDLARAIQNLQEAVRMDPEFALGWAELGSAYTRAATFGLAPKEEAIQNARGAVER
                     ALAIEPDLVEAHARLGAIRMFHDWDWRGAEASYARALELAPGDAVSLNGAGVLAMTLG
                     RIEESVALHRRASEQDPLSATPHANLGLTLLQAGRLAEAEASLRRALEIAPQRLLTHA
                     AIAKALAAQGREEEALAEAAREPDEGMRLYSLAIVHHILSHSPDADAALRELIEGHGD
                     AYAVQIAEVCAMRGELDASFEWLDKAYAQRDFGITEVQSVSSFHLLHKDPRWHSFMRR
                     LGFDEGA"
BASE COUNT         2653 a         5090 c         5192 g         2735 t
ORIGIN      
        1 gagcacggga cccttcgggt ctccgtcgag cgcttcgagg agcgcggcct cgatgcgatc
       61 gagtccgtcg aggcgctcgg cggagggtgc cgccgcgtcg gccacgtcat cggcaaggcg
      121 cgcgaacgcg tagactgcgg cgagcgcggg gcgtagcgga gacgcgatga atctggagac
      181 gaccgggaaa ttctcctcgt gacgccggac aatgtccgcg cacacgcgat aggcatcgga
      241 aagcgggacg agctcggatg cggcgggcgg cgcttcgact gggatttgcg tcacgcgggc
      301 tccctctggt gttcaaggag tagaccatgc gtcccacaga gcgtcaaggc ggggtgccgc
      361 acggagttcc ggggcgcgtg cgcttccatg gcaacgccgc gttcttgggg atggagccgc
      421 ccgaggcggg ctcccgaggg aggtaagaat gcgccgttcg gtaccgctct ggagtgccgc
      481 cctggcggcg gctccgcttg cgatcagcca tgccgacagc agtgtgaccg agctccggat
      541 cgcggccggc tcgggttcct acgcctacgt cacgcgcgga tgcgacaaca gcgtgatcag
      601 caagcagagg atcgactacg acaacgtcgg cgccgatgtg agccacaagt tcacttcgcc
      661 cgtccgcctc ggtgtgcgcg cagggcttgt acgcacccat gacaactatg actccaagtt
      721 gctctcggac acacgctacg tgaatccata tctctcgctg gagtggcccg ccctctcgat
      781 cgggggaggt tgggttggag ccaaccgccc cttccccgat caggacatcc tccagaccgc
      841 gaacgggcac gtcggaagcc aaaccggtag cgggcacatc cgaatcggaa agcggactta
      901 ctgggaggtg agctacttcg aggcggtccc gcttgcgacg gcgggctacg ggcagacagg
      961 catcggccac ctctggtcgc gcgcggatct ctggatgggt gtgggcacct ggccccacga
     1021 tcgggtcgga ttcgtgggtc gaggggaata tcggatcggg aatcggttgg ggcttggcgg
     1081 aacgctgcgg ctggggagct cgcaagggat cagcgagaac gcgttcgcgg tgtcgctctc
     1141 gtaccgcttc acgcatcgaa gagcgggaac gcaggcagcg cccgcgccgt atgatccgct
     1201 ttcggctccg tcgcggcccg agccggcgcc ggccgcgccg ccggactccg ctattgcacc
     1261 cgccgcttcc gattctgtgc gtagtccacg aagaggtagg agccgagctc ctcgagatcg
     1321 gagaagtagg cgcgtccctt gttgagctgc gtgaaccgct cgacgaagcg caccaggtag
     1381 ggatcctgcg tgaccatgaa cgtggtgatg gggatccgct tcctgcggca ggcaaccgcc
     1441 tcgtccaagg tcttgttcac gatcctggga tcgagcccga acgcgttctt gtagaagcgc
     1501 ccgttctcga agatggcgga cggcttgccg tccgtgatca tgaagatctg cttgttcacg
     1561 tgcttttgcc tcgccaggat cttctgcgcg agctggagcc ccgccttcgt gttcgtgtgg
     1621 aagggtccga ccgtcgcgta ggcgatgtcc cgaatcttca cctcccacgc gtcgtccccg
     1681 aagagcacga ccgagagcga gtccttggga tagcgggtca ggatcagctc cgagagcgcg
     1741 agcgcggcct gcttggcggg cgtgatccgg tcctcgccgt agaggatcat ggagtggctc
     1801 acgtcgatca ggagcacggt cgcgcacgat gacaggtgct ccgtctcgaa gacctcgagg
     1861 tcgtcctccg tgagtccgag cccctggtcg ccccactcgc ccctttgccg gcgcatcgag
     1921 ttccgcacgc tcgagaggaa gtcgatgtcg ttcacggagt cgccgaactg atacgggcgc
     1981 gtctcggtga tcttctcgcc cccttggccg gtgcgcggca cgcggtgctc ccccgcggag
     2041 tccttcgcga ggttgccgaa gatccgatcg agcgagtcct tccggatcat ccgctccccc
     2101 gcgcgcgtga gcacggggcg gccgtccacc tcctcgatcg cgtccttctc cttgagatgg
     2161 ttcttgaagt cctcgatctt gtacttctcg ttgaagaatc cgaaccgctc gcccagctcc
     2221 tcgagcgcgc ggagcgcctg ctcgacgttc ccgtcggtca tgaggaggag ccggtggtag
     2281 agcgagagca gattgcgctg gaagttgagg ttttgggtga gtcgctcgtc ccagcgtgaa
     2341 tagcggaaat ccatcctaga gcctctagtc ctccgcctgg tccggcttgc cgagcatcgt
     2401 cgagaacatg tcggtgtacg cgagggccgc ggtgtcggtg cgctccttgg ccaagatcga
     2461 gttctggtgg agcgcttcga gcacgaactc catcgccgtc ggaagctcgg ccggatcctt
     2521 cacggggaga tagcgcttgg gcagctcctc gaggcccgcg acctggcgga gcctctggtg
     2581 gaactcgcgg gcggggagct cgtcggagat ctcgaccttc ttcccctggg cgaaccagcg
     2641 gaagatcggg tcgtactcgg acgcggcgcc ctcgttcttc tccttcgcct tgtacgcgtc
     2701 ggggaagtac ttggtgaaga cctccttcac cgccttgccc acgagatgct tcgccaccgc
     2761 gaccgcgccc tcctgctcgc cctcgtagac cagctccacc ttgccggtga tcgacgagat
     2821 gccgttctgg aggtcgcaga tccgcacggc ttggcggcgc tcgcccgtct tgatggcgcg
     2881 acgctcggca ttcgacacga ggttctccat gagcgcgatc gtcatgcgca cgctcacgcc
     2941 gctcgactgg tcgacgtagg acgacttcct cgcctggagc gcgacgtgct ccaccagctc
     3001 ctggatgtag gacgggacga ggatctcgat cccgtccggc cgcttcaccc acgcttcctg
     3061 cgtcgtgacc ttcatcgcgt cttcgatcgt gtacggatag tgcgtcatga tctgggacgc
     3121 gatgcgatcg cgcagcggcg tgatgatgtt gccgcggttc gtgtagtcct cgggattcgc
     3181 cgagaacacg atctggagat cgagcggcag ccggatcggg aagccgcgga tctggatgtc
     3241 cttctcctcg agaatgttga ggaggcccac ctggatccgg ggctggagat cggggagctc
     3301 gttgatggcg aaaattccac gattggtgcg cgggatgatc ccgtagtgca tcacctcggg
     3361 atcgctgtag ctgagcttct ggttcgccgc cttgatcgga tcgatgtcgc cgatcagatc
     3421 ggcgatcgtc acgtccggcg tggcgagctt ctcgcggtag cgctcctcac gcgggatcca
     3481 gcggatagcg acctcgtctc cgtgcattgc gaccttgcgc tggcagacgg ggcagagggg
     3541 attcagcgga tcgtcgttga tctcgcaccc ctcgatcgcg ggcatccact cgtcgaggaa
     3601 gcggaagagc tggcgcagga tccgcgtctt cgcctggccg cggagcccga gcaggatgaa
     3661 gtcgtgcccg ctcaggatcg cgttctcgat ctggggaagg gcggtcttgt cgtagccgac
     3721 gatcccgggg aagcggtcct cgccggactc gatcgcccgg atcaggttgg cccgcatctc
     3781 ctgcttgacc gtgcgcggct ggtatccgct atctcgaagc tgcttgatgg tttgcggccg
     3841 attcatggcg tttgagggtc ctccggaggg atgggggcgc tacccgtgcg tccagcctac
     3901 tcttagatgc gctctccctc aagcgtcgcg cgccctccgg gcccctcacc acacgcaaaa
     3961 taattgttga gtttatgggg cagattgccg aataatatta ggtaatgacg actgaactcc
     4021 gaaacaaagt agaaaacggc acgctggatg ccaccgatct tcggatcctg gcgctcctcc
     4081 aggacaacgc caagatgccc caggccgaga tggctcgtga ggtcggcctc acggcgccgt
     4141 ccgtgaacga gcggatcaag aagctcgagc gctcgggata catccgcggc tacgtgacgc
     4201 tgctcgacga gcggaagctc ggccaggacg tcacggcctt cgtcgaggtg ttcatcgagc
     4261 accccaagca cgaggccggg ttcatcgagg ccgcgagcac gctcgacgaa gtcctcgagt
     4321 gccaccacat caccggcgag ttctcgctcc tcctcaaggt ccgcgtggcc gacatggggg
     4381 cgttccgccg tctcttgatc gagaagctca acaccatcga gggcgtgcgc cagacgcgga
     4441 cgctgatcgt cctcgcgacg tcgaaggagc agcaccggat caagctggat tgacgcggcg
     4501 cgagccgctc gacgctttca acaaacccga cgaaacccaa cgaaaccgcg aaaggagcac
     4561 gccatgaacc cgaccatgac cggcgcctcg gtccacgtca cgccggacca ggtgcacagc
     4621 acgctggcca agcacatgct ggccgacggc ttcgacttcg tcctcgacct cgacaagagc
     4681 cagggggtct ggctgcacga ttcgcgcggc aacaagaccc tgatggactt cttctcgttc
     4741 ttcgcgtcga acccggtggg catgaaccac ccgggcgtga agaatccgga gttccaggcg
     4801 cggctcctca aggcggcggt ccacaacgtc acgaacagcg acgtctacac gaccgagatg
     4861 gcggagttcg tggagacctt cgcgcggcac gccatgaagg acacgttcgc gcactccttc
     4921 ttcgtggcgg gcggcacgct cggcatcgag aacgcgctca aggccgcgtt cgactggaag
     4981 gtgcgcaaga acttcgcgcg cggctaccgc gaggagcggg gcacgaaggt gctccacttc
     5041 gagcaggcgt tccacggccg ctcgggctac acgctctccc tcacgaacac gaccgatccg
     5101 cggaaggtcg cctacttccc gaagttcgac tggccccgcg tgacgaaccc caaggtcgag
     5161 ttcccgctca acgaggaccg gctcaaggac gtgaaggcgc gcgaggcgct cgcggtcgcg
     5221 cagatggagc gcgcgttcga gcagaatcct ggtgacatcg ccgcgatcat catcgagccg
     5281 atccagggcg agggaggcga caaccacttc cgtcccgagt tcttcaagga gctccgccgg
     5341 ctcgccgacc agaacgacgc gatgcttgtg ttcgacgagg tgcaggcggg cttcggtctc
     5401 acgggcaagt tctgggcgtt cgagcactac ggcgtgacgc ccgacatggt cggcttcggc
     5461 aagaagtcgc agatctgcgg cttcatcgcg acgaagcgaa tcgacgaagt ccaggacaac
     5521 gtgttccacg tctcgagccg catcaactcg acctgggggg gcacgctcgt cgacatggtc
     5581 cgcagccagc gctacctcga gatcatggca gaggagcgcc tcctcgagaa cgcgacggcg
     5641 cgcggcctgg agctcctcac cggcctcgaa ggcctcgtga aggagttccc cgagcacgtg
     5701 atgaacgcgc gcggcaaggg gatgatgtgc gccttcgatc tccgggacgg tgcgcttcgc
     5761 gatcaggtga tctcgaacgc atacgccgag ggcgtggtgg ttctcggctg cggcccgacg
     5821 tccatccggt tccgcccgcc gctcgtgatc acgaaggacg agatcgcgca gggattggag
     5881 aagttccgga gcagcatcaa gaaggcgatt ggtcgctaaa ttaagcggac ggccgtagcc
     5941 gtccggaact ccgattcttt caagggggcg tcgcgcgcga gtgcggcgtc ccctttcttg
     6001 atctcatttg cgaaccggcg ggcgcgagaa accgtcagcc tacttcacca gcaccatccg
     6061 cttcgactgc accgctccat catccgtcgc gagccggtag aagtagaccc ccgacgcgac
     6121 cgggtgtccc tcggcattct tcccgtgcca gggcacttcg tgccgccccg cggcgatctg
     6181 ctgattcgcg agcaccgcca cgagccggcc ctgcgcatcg tggaccgtga gccgaatctt
     6241 ccgcgactcc ggcaacgtga accggatcgt cgtctccgga ttgaacggat tcggatagtt
     6301 ctggtggagc tggaccacgc gcggcgccgg tgcttcgctc ggctccactg cggtcaccgt
     6361 cccggtgccg cggaggttga tggccacggt cgatcctgcc gatccgacgc tcacgacgtc
     6421 gaggagtccg tcccgatcca tgtcggccag gacctcgctc tcaggggtgg cagacacggg
     6481 atagatgccg gccgaggtga acgcaccgaa ggcacccaca ctggctacct cgacccggtc
     6541 caatccggag ctcgccacca gatagggacg gccgtcggcg gccatggggg cgatctccat
     6601 gtcgaaggcg gtgaccgaaa gaaagctgat gaacgacgaa ccaaagggga aagtcgcatc
     6661 cccgaacgag acgaagtagc cattgccctg gttcgccacg atgtcgaggt agccgtcgcg
     6721 attcaagtcg ccggagcaaa cgacctgggc ggtctgggac tggaccccgg ttgtgacggg
     6781 cgtgcccaac gtcccgttgg cgtttccata gatgaccgac accacaccgt tcgcgcaaag
     6841 gacgtcgagg acaccgtccc ggttgagatc ggccaggtcg atctcatgga tcggtgcggc
     6901 cagagctaca ccgctgaatg cggtgaacgt ccccgtgccg gttcccgtga ggacccgcac
     6961 cgagtccggc gtcgaaacga ccacatccgg gatgccgtcc cgattcatgt cgcccacttc
     7021 gatgtcgctt cccacgttca tgaggtagtc cacgcgcgcg ccgaacgtga gattgcccat
     7081 gcccagcatg gtgtagatcc gtttcgcgcc ggccaccgag ctcgcaccga cgatgtcgag
     7141 gattccatcc cggttcaggt cggccagacg gactcgggtt ccataccagg actgagccag
     7201 ggtcgtcggt gacccgatgg ttccccctcc accgcctcgg tgcacggcga ctccgggatt
     7261 cgcgccttcc gttcccgtca ccacatccag cttgccgtcg cggtcgaggt cgcccacggc
     7321 gacgtccttg gggttgctca gcgtggtctt gaacactgca tcgtgcaggg atgcggtgta
     7381 ggtgggcggc gaggtcagcg cggtgtagac ctgggaggag tcgggactca cgacggcgcc
     7441 ggccatgaga tcggggatgc cgtcccggtc cacatcgccc gcggcgaggt actcgccgct
     7501 cgtcatgacg ggcggggtcc tctcgtcgat cgtgcccatc gcgtagctcg tgcgcaggcc
     7561 gaatgcgccc atcccgtttc cgagaagaac gtcaaagctg gaaaagctgc tcacgtcgcc
     7621 gatcccggtc gatcccgaag cgatgagatc ggggatcccg tcgatgtccg cgtcccatga
     7681 gtcgaggaag aaccctcgct tttccagggt tgtgtaggtg gatccgctcg gcgccggttg
     7741 aaggcccgac ggtccattga ggaagacctg aatgcccgag acaaccgaag tgccgctcgt
     7801 atccaggcgg cccgcgaggt ccacatagcc atcacgattg aaatcgcccg cgcacacatc
     7861 cttgaatcgg ccactcccga tgggaactgg gtttggcgtc caggtcccga tcccggtact
     7921 gatgtaggcg tgaatgccca ggtccgaggc gagtgcgatg tcgaggagcc catcacggtt
     7981 ccagtcggcg atcgccaagc cccagggttg ctggacaggg ccctggaact gatacagcag
     8041 catccagaac ccgaattgat tctgtgcgta gatcttcacg ttctgaccgt cgactgccac
     8101 catgtcggga tttccatcgc gcgtgatgtc tgcgatcgag cgagagtaga cgagtccgcc
     8161 aaagaggatc tggtgtggcg tgaaggtccc gtcgccgttg cccaggtacg gacggaaaga
     8221 gctcccggtc tggaccaaga catccatctt cgcgtcgcga ttgatgtcgc ccgcgaccag
     8281 ggttgtcggc tgggtcacgg tgaaggaggg accctgcgtg aacttgccgg ttccgttgcc
     8341 gaagccgatc gtgacttcgc tcatgcctga ggacgcgacg tccgggaagc cgtcgcggtt
     8401 gaagtcggcg atcgcctgcc ggtaggtgcg ctgtgcgatg cccgaccggg ttcccgaccg
     8461 gaagcccccc gacccgtcgt tcaacaaaac gccgatcgtg tgcttggttc ccatttcggt
     8521 ggccaccaga tcgaggcgtc catctcggtt cacgtcaacc aggatggcct caccatattg
     8581 gagctgaaag ttgattaaga ggtcgggcgc gctcgatcga ggaagaccgt cgtagaggac
     8641 ggggggaccg aaggtaccgt ccccgttccc cttgaccacg caaacagtcc ccgcgatgta
     8701 aggcgagttc gaagccagcg tgacgatgtc cgtgtccccg tccccatcga gatctcccag
     8761 gcatatcttc tcgggggcgt gtggaagagt gatggaaaca ccgggatcga acgtgccccc
     8821 cggatgtccc gtggagatcc gaacggcatt tgcgcagaag gtcgtcgtca cgacgtccgg
     8881 aatagcgtcg ccgtccagcg gagccacggc cagatcgagt acgcctgatc ctggcgcgaa
     8941 gggcccgaga atcgtggtga acgtacccgc gttgtagccg gttgcgacgt acatcgctgc
     9001 ttccgcgcag tccggtggat cgttgtcatc gacggctatg acatcgggta gtccgtctcc
     9061 ggtgacatcc gcaatcgcaa ggaaggacag gatgtgacga ggggccaagg gcccgagcat
     9121 cgtcgtgcca atgagctgta gggtgtccgc tgcggtatgg aagacgcggg cgaacaggtt
     9181 actcggcgcg ccggaggtca agccgacgat ctcgagtacc ccgtcgttgt cgaaatcagc
     9241 ggtgccgatc gcttcagagg cgtaggtcga ggacttggcc acgaattgct ggcccgatgc
     9301 gggggtctgg cccgtgttcc acaagatggc cgaagaggcg tagcgcgcta ccgcgatatc
     9361 cgtccggccg tccccgttga aatcgcgagc gaccatgttg agggggaggt gcagcttccc
     9421 attcggaggc acggggtagt tgaagaaggt cgcgttcaag gcggggaagt cgggctgagc
     9481 cgaggcactc cacggcaggg gcggcaggta ctgtgacccg gcatgggtga gtatggagat
     9541 ggagcccacg ctaccggcga acccgctgtc caggttggcg accgcaaagt cgggattgcc
     9601 gtccccgttg aagtcccccg atgtgatgca cacgggatat cccgtcagct cggtgttgtt
     9661 gcgtacgatg ccttcggggt acgacattga cgaccggtag agcgacacgt tcgatccggt
     9721 ggccggcggc acgttggagc agatactgaa tgcaaacgcc aagagagcaa acgagatacg
     9781 aagaaatggg cgcatcacgc ctccgccggt ggggtgtgct tcgttgcgag ctatccccct
     9841 gacggtctag accggtctac cgaacggaga tttttcggct acctgcgttt cccatgcgct
     9901 tggaagcgtc tgctacgctt ctttcatgcg acggcctctt gcgctgctcc ttgccgccat
     9961 catcgccgtc cccatcacca ccccacgaac cagccgttcg caggaatccc cggacacgac
    10021 tccgccacgg gcgggacagt cgcaggaagt cacgaccgcg ccccgcgatc aggccctcgt
    10081 cctttccggg ggcggctccc gcggcatcgc ccacgcgggt gtgatcgctg gactcgagaa
    10141 gaaggggtac agcccgggca tcgtggtggg caccagcatg ggggcggtga tcggcgcgct
    10201 ctacgcgtcg gggatgaatg ccgactcggt ctggagcgtg gtcgacatgc aggactggcg
    10261 gcagatcttc gctccgttcc cgtccccggt cgggctcgac cgctcggttc actatccggt
    10321 ccttcgttta caaggcaccg gcaactcccc agagcttcgc ggctacctgg acgactggca
    10381 gatcaaccgc cagctcgtgc gccagctctt ccgcccctcg gccgctgtgc gcggcgactt
    10441 cgatcgcttg ccgcgccgct tccgctcgat cgcggcagac ctccgcagtg gacacctcgt
    10501 gtcgatcggc tacggcgatc tcgcccgttc ggttcgcgcg agcatggcgc aggccggcgt
    10561 cttcgcgccg accgcgtggg acgatcgcgt gctcacggac ggcggtgtga gcgactattt
    10621 cccggtggcg gaggcgaggc gcctcggcgc gcgcgacgtg atcggcgtgg acgtgctccg
    10681 cccgaccgcg gagattcact cgaccgaggc gtccgccgtc atggaccggt cgctccgtct
    10741 cctcgtgcag cacgctcgcg ccgacacgct tccgcccgac attctgatcg cgcccgacat
    10801 cgatccgacg atctcaccga tcagctatcc cacggacccc gagcccttgt tcatggatgg
    10861 ctatcgagcg acgaattcgc tcccgcaggg ggcgggctct tccgagccac ccgggaccct
    10921 tcccgcgctt cccgactcac tggccgaggt gcgcgtgctc gtcgattcgc cccttcggcc
    10981 actcttgaag cgcgcgttcg atcgcttcgg caaagcccgc ttcgacgccg atcagatctt
    11041 gaagtacgtg gatcgcctgt acgcgacggg tctcttcacc ggagtgtggc cctcggtgga
    11101 gggagcaccg gcccaaccgc ccggaacgcc tgcgtcatcg gcgaggtcca cggtgacgcc
    11161 acccgcgcaa gtggcgcctc cgaccgcgcc tccagtcgag gagggacgcg agggcttcga
    11221 cggcgtcgac aacgactcga ccgaagtcga aatgcccgcc gtcggcgcac ccgccgatac
    11281 gctctccgaa cccccatccg gcgagtcatc tgcgtctccg cccgccgcgt catccgcgcc
    11341 accggacagc gccacgctct cggcgctcgg cgccgctccc gacacgtcct ccacgccggc
    11401 ggccgtgagc gatgccgttc ccgcgcttct catccgcgcc gagacggtgg gtgctctctc
    11461 ggtgaacggt gcggtgggtt acgacaacga tcgcggaggc cggggctggg ccaatctgag
    11521 aaagctcgga gcgatgggat cgcatacggt cgagttcgcg ctcgaggcga tggccaatgg
    11581 catcgaacag tccgccagca tgaacgtgcg cgtgccgacc gtggatcgcc tgccaaccgc
    11641 gtggagcggg ggactctacc tcgcggagtc gcagatccgg ttcctcagcg tgaggcccga
    11701 cggggatgac cctgatgtga agcggggcgg cggatggatc ggagccgagg ttcggaagat
    11761 cagcccggcg atcgaggcga ccgcgatctt cacagcggaa aatctcgacg cgaatgtcgg
    11821 cccgagcggc gactccttcg gaccgatggt ccggatcggg ttcatccccg acaacctgca
    11881 gctcgtcggc attccgacga gcgcggaagg ggaagctcga accggtgacg cgcgctacgc
    11941 ccgtgtgcgc gcgcgcggat cgcgcgtgtt cgcgttcaag aagctcaccg tcgcgcctct
    12001 gttcgacgtc gcggcggtga cgcagggcac cccgcgcgac gtcgtgcctt cgcccggcat
    12061 ggaccagctc gtgcctgcgc tccgctgggg cgagcgccgc gggcgggctc aggcgatcgc
    12121 cggcgtggac gctgccttcc cgctcttcac caagattcgc gtgaggcttc gtggcggcgg
    12181 atcggcggac cagctcctgc cggacggatc ggatttctcg agcgcgacgc tctggttcgg
    12241 cggcgcgtcc gtgtccgcgc tctggtggac gccgttcggg aggatcgagg gtggcttgga
    12301 ggcgaatacg ctgggcgatc gaagggcggt cgtgagcgtg gggcaggact tctaagccgc
    12361 ggggccaggc ctcgaatggc ccggcgagct caccttcctc atcgcgatcg ccagcttgtg
    12421 caggctccgc tgaatcgcca tccgcgccgc gtccgcgctg ggcctgtcgg tcatcgcggc
    12481 gatatcctcg tagcccatgt cgagctcgat cctgaggtga aggatctgcc gttcgtcctc
    12541 cgataggagt gacatcccct cttcgtaccg ccgcacgacg tcgctcccga tggcttcctc
    12601 gagaggtgat ggagtcgggt ccacgaggct ctcgaggttg ccatcgccac gccgccgagc
    12661 ccaccggatc tggtctcgaa ttcgattcag gaccgcgtgg cgcacgtagg cctcgaagct
    12721 tcctggatgc cgggctccga tcgagccgat ggtctccagt gctcgaagca gcgtctcctg
    12781 cacgaggtca ccggtatcga gcagagaccg cgcattgcgg ggcaggcggc cgctcgccca
    12841 tcgttcgaga cgggggcggt atcgcgcgag gagcgtctcc agcgcatcgc gccgcccctg
    12901 ttgggcctcc cgaaggaggt cttgggtccg gaggagatgg tggttgtccc ggctcacgcg
    12961 ccctcgtcga accccagccg cctcatgaac gagtgccagc gcgggtcttt gtggagaagg
    13021 tggaagctcg aaaccgactg aacttccgtg attccgaagt ctcgctgggc gtacgccttg
    13081 tccagccatt cgaacgaggc gtccaactca cctcgcatgg cgcacacttc agcgatctgg
    13141 accgcatagg catcgccgtg tccctcgatg agctcacgga gcgccgcgtc cgcatccggg
    13201 ctgtgactca gaatgtgatg aacgatcgcc aagctataga ggcgcatccc ctcgtccggc
    13261 tcccgtgccg cttccgccag tgcctcctcc tcccgccctt gagccgcgag ggctttggcg
    13321 atagcggcat gagtgagcag ccgctgtggc gcgatctcga gcgctctccg aagggaagcc
    13381 tctgcctcgg ccaggcgacc ggcctgaagg agcgtgaggc ccaggttggc gtggggggtc
    13441 gcgctcagag ggtcctgttc cgacgcccgg cgatggagcg cgacggactc ctcgattcgg
    13501 ccgagcgtca tggccagaac ccctgcgccg ttcagggaca cggcgtctcc tggcgcaagc
    13561 tcgagcgctc tcgcgtacga cgcctcggcc cctctccagt cccaatcgtg gaacatgcgg
    13621 atcgcgccca agcgcgcgtg cgcttccacc agatcgggct cgatcgcgag tgcgcgctcc
    13681 acagcgcccc gcgcgttttg aatcgcttcc tctttcggcg cgagtccgaa ggtggcggcc
    13741 ctggtgtagg cggagcccag ctcggcccag cccaaagcga actccgggtc catcctcacg
    13801 gcttcctgga ggttctggat cgctcgcgcc aagtcctcgc ggttgagccg agtgatgaag
    13861 taccggccca gaagatagag ccggtgcgcc gccgggtccg tcgctcgacc cctggccgca
    13921 cgcgctatct ccgcgttcac gtccggcccg gtgtcaccca ggagcgcggc gcgcagctcc
    13981 ttgaccaccg actgtgcgat gtcgtcctgc acggcgaaga tgtcatccag cgttcgatcg
    14041 taggtttccg accagaggtg gagcccttcc gaaaccttga cgagctgaac cgagatccgg
    14101 acccggtctc ccgagcgtcg gatgctcccg tcgagcacgg tgtcgacgtg gagtgcgcgg
    14161 cctatctcct cgacggtcgc ctggcgacca cggaagctga acgcggacgt gcgcgccgcc
    14221 acgcggagtc cacggatctt tcccagcatg ccgatcagct cgtccgcgag cccgtcggag
    14281 aaatactcgt cctcggttcc ggggcttcga ttgacgaagg gaagcaccgc gatcgagagg
    14341 gttcccgcgt gggcggcggt cccgagcccc cgggcgatcg tctgaaaccg gccgcccagc
    14401 tccgccgcgg tgcggacccg atcggagcga gacggcgcca tggccgcgag gatggcgtct
    14461 tcgaaatcct tcgagacttc aggccggatc gagcgaaggg acggtgaggg gcctcgaggc
    14521 gcttcctccc tgagctcggc caacgttctg gccttgaacg gaggccgccc ggcgagcgcc
    14581 caccacagcg tgactccgag cgcgtacaga tccgactgag gggtcggcct ctcacccgcg
    14641 agcagctccg gcgccatgaa caagggcgtg ccggagcttc gcggcgtctc ctcgtcgtcg
    14701 agcgccggcc gccaaccgag gccgaaatcg gtgagcacag cgcggtcctc acgctcgagg
    14761 aggatgttcg cgggcttgat gtcgcggtgc acgaggccgg cagcatccag cgcctcgagc
    14821 gcggagcaga gctgggcgcc gatcagagcc acctgggatg ggcggagcgc gcccacgcgc
    14881 tcgatttctc gagcgaggct ggtgccgggg atgcactcca tccacattcc gaaacgaccc
    14941 tggtcctccg cgatcccgaa cacggtgacg acgccagggt gccggatgcg cgccagggcg
    15001 cgggcttcgc tcagaagctc cgccgaggcg gactcgcccg gggcggtttg caggaacttg
    15061 agcgcgactt gccgccggag ggtggaatcc cacgcgcgcc acacctcgcc ccgtgcaccg
    15121 gcgccgagcg gctccatcag cgtgagatcg cgccagcgct cgaggggtgg gtcttgggga
    15181 ctcgggggcg aggacgaagg ggtcgcggca cgctgcagcg ctcggttgta ctctgcgatc
    15241 cgagcgacgt cgcgcatcat ccgtaccgtg tcggaatcga gctcggcatc gagctcgaca
    15301 tgatcccagt cgaccggctt gccgtccgag acagacccgg tgagacggtc gacgttgtcg
    15361 ccaggttcca cggagcccct tcgtagaaac cggaacggca cgtgcgaagg cgggacacaa
    15421 gccccgcatg agggcgaatg gcagcccgga gatgggttat tttagcagat cagccttgga
    15481 tctcggtaag cgatgaagga caggaccaac gggagtgtcc atggccggga aagcggtcag
    15541 gaagtcagtt ggagtgcgga ggtctcgcaa aaccgcgaag acacggtcgc ccgaggcgcc
    15601 gcggtctacg aagccgaagc tcctcgcggg cggcaatcct cagatcgcga aggcggaggg
    15661 cgatgctccc
//
LOCUS       DASTKC010000162         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1782548, whole genome shotgun sequence.
ACCESSION   DASTKC010000162 DASTKC010000000
VERSION     DASTKC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782548"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VFR25_06380"
     CDS             complement(<1..1363)
                     /locus_tag="VFR25_06380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET9940709.1"
                     /translation="MSEALASAFERLERRVPAFERRAGQLTMAGLWETVLERGGVLAV
                     EAPTGIGKSLAYLVPALLRRRVGCGPILVSTHTKALQDQLLTRDAPLAASAIGSPFRG
                     AILKGRAGYLCRRRAASRFAQRRLFAGAHADESMLDALEAWVDRTATGDIDELRDEGI
                     EAPASLLAEIASDPLFCSGSGCDPGGGCFAKAARREARRSDLVIVNHALLLSDPGLRA
                     TLLAESGALILDEAHQIERVAREQLGVSLGVRDLARLAARTDARSGALKLLARTIRRG
                     KGASVTERIVTADQAVRPVLDHAAALSRDLEAILPPGAASARLTRDDDLAAISPVALD
                     LLLTSAAALVRALERVVEAAESETGGAFRPEASEAIDEVRARHAAWLEVEQAIRAVTN
                     LEEKGAAFYVDKDERGAPRWNKRPLEVGVPLRSQLFETSACTLLTSATLTPGSDFEPF
                     LASI"
     gene            complement(1431..3452)
                     /gene="ligA"
                     /locus_tag="VFR25_06385"
     CDS             complement(1431..3452)
                     /gene="ligA"
                     /locus_tag="VFR25_06385"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9940710.1"
                     /translation="MSARAEQKIRSLRNEIARHDHLYYTIGEPEVSDREYDALMKELE
                     ELERAHPELADPDSPTSRVAKGLLTGFPTVRHTAPMLSLDNTYSLDEVSEFDARVRKS
                     LGKDRIDYSVEPKVDGVAIHLRYERGRFVLGLTRGDGERGDDITANLRTIRSIPLRLT
                     GRNVPDLVEVRGEVYMETVAFQKMNERRAADGKPTFMNPRNATTGSLKMLDTTEVSRR
                     PLQMFVYQLLHPDRYGIDQHLEALALAKKLGLRVNPRNSLAKGYEDLRKQCEAWGEEW
                     GSLAYGADGLVIKVNDLEDARRLGTTSKSPRWGIAYKFGTTEAETTLRKIDLQVGRTG
                     VVTPVALLEPVLLLGTEVKRATLHNQDEIDRKDIREGDRVVIEKGGEVIPKVVRVVAH
                     SGRRGPRFVMPSTCPVCGTTLTKDPEQAAVRCENLYCPAQVRRRIQYYASRGALDIEG
                     LGERTVDQLVDAKLVDDPADLYDLTVDELAELDGMAEKSAGNLVRGIAASKDAPLDRL
                     ITAIGMRHVGNTVARLLADHFRSLAALSDADEEALLEIQGIGPEIAASVVAFFKSREG
                     RSLVKRLTERGVKGKAPERRAATAGPFAGKTFVLTGTLRMPREEAEELILRGGGRVTS
                     SVSKKTDAVVVGEDAGSKLDKAKALGVPTWDEARFRAALREAGLTGAAR"
     gene            complement(3449..>4443)
                     /locus_tag="VFR25_06390"
     CDS             complement(3449..>4443)
                     /locus_tag="VFR25_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9940711.1"
                     /translation="ASQFEGALGVAPGEGLTGRVDLGLGNIAGTGRAAGARWVGFGNG
                     RSLYALHYKEPSLFGRALDASLALDADLADSLFSHTGWSIALGGRPAARTRLEAGVER
                     SGTVYTGLSRGSSSTWSVRGGVGWDRLAPIMNPVRGVSLSLDAEGGRRTDAFPGFEES
                     RRGVMRGKAELASAASLGGSRVLFGSVRAEEVRLGDGDFPIEELCYLGGSEGLRGHRD
                     RAFAGNRILAMTLEHRWITDPRGGRAYLFVDGGAHALDASLQAGVPAGTVDASAASAS
                     TTLSRTELSDGWELGYGAGLQTRMASGLMGVELGLRPGAALREATIHIRYASRW"
BASE COUNT          624 a         1594 c         1550 g          675 t
ORIGIN      
        1 cgatcgacgc gaggaacggc tcgaagtccg atcccggcgt gagcgtcgcg gaggtgagga
       61 gcgtgcaagc gctcgtctcg aagagctgcg accggagcgg cactcccacc tcgagcggcc
      121 tcttgttcca gcgcggagcg ccgcgctcgt ccttgtccac gtagaacgcc gctcccttct
      181 cctcgaggtt cgtgaccgcg cggatcgcct gctccacttc gagccacgcg gcgtggcgcg
      241 cgcgcacctc gtcgatcgct tcggacgctt cggggcggaa cgcgcctccc gtttcggact
      301 cggccgcctc gacgacgcgc tcgagggcgc ggaccagcgc cgcggcggag gtcagaagga
      361 gatcgagcgc gaccgggctg atggccgcga gatcgtcgtc gcgcgtgagc cgcgccgagg
      421 cggccccggg cgggaggatg gcctcgagat cgcgcgagag cgccgcggcg tgatcgagga
      481 ccggccggac ggcctggtcc gcggtcacga tgcgctccgt gacggatgcc cctttcccgc
      541 gccggatcgt cctggcgaga agcttgagcg ctccgcttcg cgcatcggtg cgcgccgcga
      601 gccgagccag atcgcgcacg ccgagggaca cgccgagctg ctcgcgcgcc acgcgctcga
      661 tctggtgcgc ctcgtcgagg atcaacgccc ccgactcggc gaggagcgtc gcgcggagcc
      721 cgggatcgga gaggaggagc gcgtgattca cgatgacgag atcggagcgc ctcgcctcgc
      781 gccgcgcggc cttggcgaag cacccgccgc ccgggtcgca tcccgacccc gagcagaaca
      841 agggatcgga ggcgatctcg gcgaggagcg aggccggagc ctcgatcccc tcgtcacgca
      901 gctcgtcgat atctccggtc gccgtgcggt cgacccacgc ctcgagcgcg tcgagcatcg
      961 actcgtccgc gtgagctccg gcgaagaggc gccgctgcgc gaatcgcgat gcggcgcgcc
     1021 ggcggcagag gtagcccgcg cgccccttca agatcgcgcc gcgaaacggc gatccgatgg
     1081 cggaggcggc gagcggcgcg tcccgcgtca ggagctggtc ttgaagcgct ttcgtgtgcg
     1141 tgctgacgag gatgggtccg caaccgactc ggcggcgaag gagcgcgggc acgagatacg
     1201 cgagcgattt cccgatgccg gtcggcgcct cgaccgcgag cacgccgccc cgctcgagga
     1261 cggtctccca tagccccgcc atcgtgagct ggccggcgcg gcgctcgaac gccggcacgc
     1321 gccgctcgag gcgctcgaac gcgctcgcaa gcgcttcgga cattgtaggt tcggaagttt
     1381 tcatgggtcg gagattcaac ctccgaccct acgggacgag gccccgtctg tcaacgagcc
     1441 gcgccggtca gtcccgcttc ccgaagcgcc gcgcgaaagc gggcttcgtc ccaggtcggg
     1501 acgccgagcg ccttcgcctt gtcgagcttg cttcccgcat cctcgcccac gaccacggcg
     1561 tccgtcttct tgctgacgga cgaggtcacg cgccctccgc cgcgcaggat cagctcttcg
     1621 gcttcctcgc gcggcatgcg aagcgtgccc gtgagcacga acgtctttcc cgcgaacggt
     1681 cccgcggtcg ccgcgcgccg ctccggcgcc ttccccttca cgccgcgctc ggtgaggcgc
     1741 ttcacgagcg agcggccctc gcgcgacttg aagaaggcga cgacgctcgc ggcgatctcg
     1801 gggccgatgc cctggatctc gaggagcgcc tcctcgtccg cgtccgacaa cgcggcgagc
     1861 gatcggaagt gatccgcgag gaggcgggcc acggtgttcc cgacgtggcg cattccgatc
     1921 gccgtgatga ggcgatcgag cggagcgtcc ttcgacgcgg cgatgccgcg cacgagattg
     1981 cccgccgact tctcggccat gccgtccagc tcggcgagct cgtccacggt gagatcgtag
     2041 agatcggcgg ggtcgtcgac cagcttcgcg tccacgagct gatcgaccgt gcgctcgccg
     2101 agcccctcga tgtcgagggc gccccggctc gcgtagtact ggatgcgccg gcgcacttgc
     2161 gccggacagt agaggttctc gcagcgcacg gccgcctgct cgggatcctt cgtcagcgtg
     2221 gtcccgcaga cggggcaggt ggacggcatc acgaaccgcg gtccgcggcg tcccgagtgc
     2281 gcaaccacgc gcacgacctt ggggatgact tcgcccccct tctcgatcac cacgcgatcc
     2341 ccttcgcgga tgtccttccg gtcgatctcg tcctggttgt ggagcgtggc ccgcttcacc
     2401 tcggtgccga gaaggaggac gggctcgagg agggcgacgg gagtgacgac gcccgtgcgc
     2461 ccgacctgga gatcgatctt tcggagcgtg gtctccgcct cggtggtgcc gaacttgtat
     2521 gcgatccccc accgcgggct cttcgaggtc gtcccgagac ggcgcgcgtc ctcgagatcg
     2581 ttgaccttga tcacgagccc gtccgcgccg tacgcgagcg atccccactc ctcgccccag
     2641 gcctcgcact gcttcctcag atcctcgtac cccttggcga gcgagttgcg cgggttcacg
     2701 cgcaggccga gcttcttcgc cagggcgagc gcctcgagat gctggtcgat cccgtatcgg
     2761 tccgggtgga ggagctggta gacgaacatc tggagcggcc ggcggctcac ctcggtcgtg
     2821 tcgagcatct tcaagctgcc ggtcgtcgcg ttcctcgggt tcatgaaggt cggcttcccg
     2881 tcggccgcgc ggcgctcgtt catcttctgg aaggccacgg tctccatgta gacctcgccc
     2941 cgcacctcca cgagatcggg gacgttccgg cctgtcagcc taagcgggat ggagcggatg
     3001 gtccggaggt tcgcggtgat gtcgtcgccc cgctcgccat cgccgcgcgt caggccgagg
     3061 acgaagcgcc cgcgctcgta gcggagatgg atcgcgactc cgtcgacctt cggctccacg
     3121 gagtagtcga tccggtcctt cccgagcgac ttccggacgc gggcgtcgaa ctccgacacc
     3181 tcatcgaggg agtacgtgtt gtcgaggctc agcatcggcg ccgtgtggcg cacggtgggg
     3241 aatcccgtga ggaggccctt cgccacgcgg gacgtgggcg agtcggggtc cgcgagctcg
     3301 ggatgggcgc gctcgagctc ctcgagctcc ttcatgagcg cgtcgtactc gcgatcggag
     3361 acctccggct cgccgatcgt gtagtagagg tggtcgtgcc gcgcgatctc gttcctcagg
     3421 gaacggatct tctgctcggc ccgtgcgctc accagcggct cgcgtatcgg atgtggatcg
     3481 tcgcctcccg tagcgcggcg cccgggcgga ggccgagctc gacccccatc aaacccgacg
     3541 ccatgcgcgt ctgaaggccg gcaccgtacc ccaattccca gccgtcgctc aactccgtcc
     3601 gcgagagcgt cgtcgacgcc gaagccgccg aagcgtcgac cgtgcccgcg gggacgcccg
     3661 cctggagcga cgcgtcgagc gcgtgggcgc cgccgtcgac gaagaggtac gcgcgaccgc
     3721 cgcgcggatc ggtgatccac cggtgctcga gcgtcatcgc gaggatccgg ttgccggcga
     3781 aggcgcggtc ccggtgccct ctaagcccct cgctccctcc gaggtagcaa agctcctcga
     3841 tcgggaagtc gccgtcgccg agccgcacct cttccgcgcg caccgatccg aagaggacgc
     3901 gcgatccgcc gagcgacgcg gcggacgcga gctcggcctt gcctcgcatg actccgcggc
     3961 gggattcctc gaatccgggg aacgcgtccg tgcgccggcc gccttcggcg tcgagcgata
     4021 ggctcacgcc gcggacagga ttcatgatag gcgcgaggcg atcccagccc acgccgccgc
     4081 gcaccgacca ggtcgagctc gagccgcggg agagcccggt gtagaccgtg ccgctccgct
     4141 cgacccccgc ctcgaggcgc gtgcgcgccg ccggcctgcc cccgagcgcg atcgaccagc
     4201 ccgtgtggct gaagagcgag tcggcgaggt ccgcgtccag ggccaggctc gcgtccagtg
     4261 cgcgcccgaa cagggacggc tccttgtaat gaagcgcgta gagcgagcgg ccgtttccga
     4321 agccgaccca gcgcgcgccg gccgcgcggc cggtgcccgc gatgttcccg aggccgaggt
     4381 cgacgcgtcc ggtgagtcct tcgcccggtg cgacgccgag cgctccctcg aactggctcg
     4441 cac
//
LOCUS       DASTKC010000163        14990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_837635, whole genome shotgun sequence.
ACCESSION   DASTKC010000163 DASTKC010000000
VERSION     DASTKC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837635"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /gene="fabG"
                     /locus_tag="VFR25_06395"
     CDS             <1..737
                     /gene="fabG"
                     /locus_tag="VFR25_06395"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET9940712.1"
                     /translation="FEGRVAVVTGGAKGIGRAIAVHLASGGAKLVLAGRSEGPLEELK
                     AEIHGQGGEVVAVRADVAVEADADALCARTLEAFGKAEILVNNAGVTRDGLLLRMSDA
                     DWDQVLDTNLKGAFHCTRAFARPMVRQKWGRIVNIASVVGLTGNAGQANYSASKAGLL
                     GLTKAVAKELASRHITVNAVAPGFIETAMTDALGDKVRDGLKAQIPMGRLGKAEDVAQ
                     AVGFLCSEGAAYVTGQVLTVDGGMVM"
     gene            799..1047
                     /gene="acpP"
                     /locus_tag="VFR25_06400"
     CDS             799..1047
                     /gene="acpP"
                     /locus_tag="VFR25_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006993870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9940713.1"
                     /translation="MAAFSEERVKQIIVDQLGVAPEQVTAEASFIDDLGADSLDTVEL
                     VMALEEEFDLEIPDEDAEKMTTVADAIKYLEGHVPKNA"
     gene            1123..2382
                     /gene="fabF"
                     /locus_tag="VFR25_06405"
     CDS             1123..2382
                     /gene="fabF"
                     /locus_tag="VFR25_06405"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9940714.1"
                     /translation="MSQSNGQPRRVVITGMGVVSPLGSDVEVFWKRLTAGESGIGPIT
                     RFDVTKYDTRFGGEVREFRAEDYLDKKDIRRADLFVQYAIGATAQAVKQAGVSPETVD
                     PNRYGVVVGSGIGGIATFEDQHRTLLEKGPSRVSPFFIPMMISDMASGQVSIQFGAKG
                     PNYCTVSACSSGAHAVGDAFRIIQSNEADVMISGGAEAPIAPVAFAGFCSMKAMSTRN
                     DDPLKASRPFDAQRDGFVMGEGAGIVVLEELEHAKRRGVKILAEIVGYGATGDAYHMT
                     APAPEGEGAGRAMRAAIRDSGLSLESFGYLNAHGTSTPLNDKFETQAIKSVFGDRASK
                     LPVSSTKSMTGHLLGAAGGLETIICVLALERQTIPPTINYETPDPDCDLDYVPNTARA
                     VELQAALSNSLGFGGHNVTLALSRYMA"
     gene            2410..3837
                     /gene="rnc"
                     /locus_tag="VFR25_06410"
     CDS             2410..3837
                     /gene="rnc"
                     /locus_tag="VFR25_06410"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET9940715.1"
                     /translation="MNFLRKLFGLDQPAAPKAPAPVRSRDRDFDLKGLERILKYQFKD
                     AGLARTAMTHRSYLHASPGRSGESNERMEFLGDSVVGLAVNEFLYNKFPKLREGELTK
                     MKSLLVSRVILSRTANLMGLGNYILLSEAENGSGGRGRASILADTLEGVIGAVYLDGG
                     LEPARKLTERLLLREVHEILSDANLANYKSMLQEYVQGEFKTHPQYRISSENGPDHQK
                     MFAVEVVVNGKTLGRGHGNNKKEAEQEAARDALLHFEKLVRPDRGGEEAGSAGRRRRR
                     GGRGRRGGRSGEGRMEDGSSGGESRGRGGREGGRDREPREGREPREGREPREGREPRE
                     GREAGSSRDGGEAREGREGREGRRQRGGRDRRGRGSREPQATREPREETTRDVYPPQR
                     EVKPRDNEPYEAPEPRVEREPEVPREHSEPRPYDEPRDRGESFDRGSSGGSSSEGSEN
                     GGGSSEKNFSPNRDPWAASRLHTDS"
     gene            3927..5579
                     /locus_tag="VFR25_06415"
     CDS             3927..5579
                     /locus_tag="VFR25_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940716.1"
                     /translation="MNVQKRAALAALTFTLALAPSPARAYFFSLYTPPTVTCQIQSEL
                     AEAAGDHAGAIAWAESLTVLDPQSSYAASRVASLYEDGGEDEMALHWGDRALSLDSLN
                     VEAAMLVGRMRLRSGEPGLAVQALTPPLRLVGAIPELYALRSLAHELNRNYEAALADL
                     KRTDVLLPDFAWIATGILSLSLEDGRLEEASSALHLALELNPDDSRTLRLGVALARRN
                     GDPLLEGRLLKQLALADEPRPEDVASYAAFLMRSRNEAELGGLFQRAEAKGLNGDDVR
                     VDAGQELLRFGDYAHALEAVKPLKKDTRAVPIRARAYLALGEEKKALEGYRRLLPGRT
                     LVREESLIVAYLEIRVGDRNEGIRTLEDTRSRPFDTPRQVLAASLCYSLLGHPEEALS
                     LVRESAARGIDSPSIYEELGSAATVLGDSLLAQWAWERLRDMGQETSECLFFLASSEL
                     GQGDQAKGMATLTRSIQLDPKNGRALLLMGKLRHRLGQLEMAREILIRSARCPETAAE
                     ANRELAKVCRTLRLDSEARDAESRARSGRPAPPAGLSLFQNP"
     gene            5647..6270
                     /locus_tag="VFR25_06420"
     CDS             5647..6270
                     /locus_tag="VFR25_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9940717.1"
                     /translation="MPIEPLRLKKLDLAELTDEDVMERCAMGSEAAFRTLVQRYRTRI
                     MNLVCRFINDRDRAEEISQEVFLRVFRNRERYRKSGKFSTWIFTIAVNLTKNEIRSRV
                     RHRGTFSLDAMEEESSGQGVTFPDAKPLPDEDLNAHEIGAKVAEALRKIPARYREAVV
                     LRDVEGLSYEEVGQILRIPGGTVRSRINRARLMLKERLKPYLTLEDV"
     gene            6267..6974
                     /locus_tag="VFR25_06425"
     CDS             6267..6974
                     /locus_tag="VFR25_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9940718.1"
                     /translation="MNCRSAESLFSAFVEDELNQKERRFLESHLLSCRRCTASMRELK
                     ASMELCAALPDVDTSPHFEDDVMAMIRNGEALRPSLIEWLRGLVTIERIRPFALAGAG
                     ASAVLIGSFVINTLHLNSSAVVADRTTKANQTQPATPSGVPTQEEVAATTMPEATAPA
                     PSAPAAVKSDAGYAWAGHSVRTSSIPDTTVPNPGARYEDEYITDQFILERGTDGGNNP
                     TITPVSSRASDDVYITF"
     gene            6982..8178
                     /locus_tag="VFR25_06430"
     CDS             6982..8178
                     /locus_tag="VFR25_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9940719.1"
                     /translation="MSQPRHKRGAAVLAFSVLVLAGPGAGVSHAVPRHVAFRSDEASL
                     LGVVDSVHASIVTVVGYPPPDPSGHAGKRRRLIGSAVAMAPDRIIASASVAIPGGSVR
                     VLLGNGVERPAILRGVDRQSNIALFQVEGAQLEPLHAAPPQSPAVGSWVAVVSNVGIT
                     RPQAAIGQIVGRGERVDEHTGGDIFEIDAPTYPGSTGAAILNEDGEWIAMVVGRAAPS
                     TSDGSPVGLDSGESMPGPSSVLIALPVDQVMYITKELDTYGSVRRGFLGVQLKRGPAS
                     PSDSLGILVANVVPGSPADSSGLRVGDRILAVDGQEAHTADDLTAIVRAMRPGDDVEL
                     TVLRKDDIFPIRVVLGAAFTNPPVGPRPSREEEIRRAKTDLNRLEMEKRQLEQRLRTL
                     EGSSSR"
     gene            8345..9451
                     /gene="aroB"
                     /locus_tag="VFR25_06435"
     CDS             8345..9451
                     /gene="aroB"
                     /locus_tag="VFR25_06435"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET9940720.1"
                     /translation="MTRIRVRPKRVAYDVTVAAGILARAGSLLRRTHRGSTAVVVTDR
                     TVDRLHGARLVRSLTSAGFIVRRDVLPDGERAKSVATLERLWDRWSRSGLSRDAIVVA
                     LGGGVVSDVAGFAAACYARGLPWAAFPTTVIGQADAAIGGKTGVNLALGKNLVGAFHH
                     PVAVYADVDTLRTLPTRAYRAGLAEIAKIGVIARPGILPKLERLARAGTRAARPTLVA
                     PLVRSAAEAKATLVERDERDQGARLALNFGHTVGHALEAATGYRRYLHGEAVAIGMAA
                     ALRMSVLEAGLDPVDAMEAESLLTALGLPTRLSREPGRAFWMALARDKKRGRSGVRMV
                     LCPAIGQSKVFELPALTTLRRVVRSLVRHPGGNP"
     gene            9448..10368
                     /locus_tag="VFR25_06440"
     CDS             9448..10368
                     /locus_tag="VFR25_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940721.1"
                     /translation="MRGDAPKETALPVEEIIAKDPSSALYLPYAERLVEDGRLEEAIA
                     LCEERKRRPGRGVGDHIVLGRAYLADGQLGPARAEFKEALDLDRENVVALKALGGILA
                     HEGNHTEAASYYQAVCRVDPGDLESQTALHQITSGEYPEIRPADVIVGQGAMSWQPVR
                     LPREEEHLSELALGLRTIERFDASQTERTAPRPYTAPEGDFDLPVERNAPEEARAAIE
                     PRDLTAPIAEHLALSDMAADAASESDADELSPEPAPAHAEAHVAAGPSGLGEIVESQP
                     PIEVPRAPGKVVEGNKNAFETWIRQLGGKE"
     gene            10391..10837
                     /locus_tag="VFR25_06445"
     CDS             10391..10837
                     /locus_tag="VFR25_06445"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9940722.1"
                     /translation="MLHGPNLGLLGRREPNLYGKETLASIDRRLVELGEALDLRVSSF
                     QTNHEGALVDRLTVAMDEVDGCLLNPAAFSHGSLALADTLRSMTIPVVEVHMTNLYAR
                     ERERQFSLTGSAVTGVIMGFGPASYELGLHALAAMIAPARGRRKRT"
     gene            10834..11280
                     /gene="accB"
                     /locus_tag="VFR25_06450"
     CDS             10834..11280
                     /gene="accB"
                     /locus_tag="VFR25_06450"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET9940723.1"
                     /translation="MSLKRDIQELVQIMREEDLAEIEVRRWFSSVRVRRPMSEQALVH
                     APAMAPAAPASAREAEASAESQLTPIKSPMVGTFYRSPAPDADPYVEENSSVDVGQTV
                     CIVEAMKLMNEIESDVRGRIAKILVENAQPVEFGQVLFLVEAESAA"
     gene            11303..12424
                     /locus_tag="VFR25_06455"
     CDS             11303..12424
                     /locus_tag="VFR25_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET9940724.1"
                     /translation="MNIKRVLQRMVEMGASDLHVKVGTKPTARVNGELIPIDEAPPSQ
                     ADLETVVDQILTPKQKRVFEETKEVDFAFGVTGLSRFRTNFYQQRGTFAMVFRQVPAT
                     IPAFEDLNLPSVIEDLALKPRGLVLVCGTVGSGKSTTLAAMIDSLNRREPRNVITIED
                     PVEFLFRDKKCTISQREVGLDTDSFGEGLKHILRQDPDAILIGEIRDPVTMRTALMAA
                     DTGHLVLSTLHTIDAAQTVNRIISFFPPHEHEEVRYMLSTTLQAVVGLRLIPRADQSG
                     RIPAAEVLIVTATVREYLLDPEKTSLIKTAIADGVSQYGMQTFDQSIMRHYSEGKISI
                     ENALKYCSNPTEFELRVKGIHATSDETWKTFERPKQETL"
     gene            12430..13788
                     /gene="accC"
                     /locus_tag="VFR25_06460"
     CDS             12430..13788
                     /gene="accC"
                     /locus_tag="VFR25_06460"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9940725.1"
                     /translation="MFRKILIANRGEIALRIVRAAREMGIETVAVYSEADRDSLHVRL
                     ADEAVCIGPPPSSASYLNMARIISAAEITAADAIHPGYGFLSENAHFAEVCESCHIRF
                     IGPTSEMIRTMGDKAVARKTAVAAGVPVTPGSEGVLESPDQAADVAKSLGYPVIIKAA
                     AGGGGKGMRIATDEASLRNGVMMAQAEAEANFGSGAVYLERYVEKPRHIEFQVFGDKQ
                     GNVVHLGERECSIQRRHQKLIEEAPSVALSPELRARMGEAAVRAASSIRYEGAGTIEF
                     LLGPTGEFYFMEMNTRIQVEHPVTEMVTRHDLIKAQILVAAGEPLPWKQSEIQLHGHA
                     IECRINAERPDQNFQPSPGTVRYFHSPGGPGVRVDSHLYSGYRVPPNYDSMVAKIICW
                     GQNRSEAIARMRRALLETVVEGIDTTLSFHLEVLADPGFQKGEIHTGYLEEFMSQRTK
                     AA"
     gene            13785..14561
                     /locus_tag="VFR25_06465"
     CDS             13785..14561
                     /locus_tag="VFR25_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET9940726.1"
                     /translation="MTSTGARGASGIARIDLYLTPSEVERAPLEDRRLVVVDVLRSST
                     SIATALQNGASKIIPVETVEAATRLAPTLDPKSRLLCGERDGRKVGGFDLGNSPREYT
                     RERVEGATLVFASTNASPLMAGPLDGREQWILGYINLGAMAELTRSEGADLAILCAGK
                     SGRFSLEDAACAGALIRRLAGDGDSIVLNDAAEMAREYDRRHGADPEGILRLSEHGRY
                     LIELGFEEDLPVCARIDSVPLVPLLKDGRITAGAAPASRR"
     gene            complement(14558..>14990)
                     /locus_tag="VFR25_06470"
     CDS             complement(14558..>14990)
                     /locus_tag="VFR25_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET9940727.1"
                     /translation="HAAQFQELVIPGLLEKPEDFAKAEEDPMALSYWQLEKYIGKLKQ
                     SGSRVQKYLVELYLKVAFPLTNLIVVVIGTALGLRVRRGGFAISFGLSIFISFVYYAF
                     IRTGQSLGHNGTLPPMLAAWLGNLFFGVVALELLRRARRGG"
BASE COUNT         2539 a         5033 c         5168 g         2250 t
ORIGIN      
        1 cgttcgaggg acgcgtcgcc gtggtcacgg ggggcgcgaa ggggatcggg cgggccatcg
       61 cggtccacct cgcgtcgggg ggcgcgaagc tcgtcctcgc ggggcgctcg gagggaccgc
      121 tcgaggagct caaggccgag atccacggtc agggaggcga ggttgtcgcg gtgcgggcgg
      181 acgtggcggt ggaggcggat gccgacgccc tctgcgcgcg cactctcgag gctttcggca
      241 aggccgagat cctcgtgaac aatgccggag tgacccgcga tggcctcctc cttcgcatga
      301 gcgacgccga ctgggatcag gtgctggaca cgaatctcaa gggtgccttc cattgcaccc
      361 gcgcgttcgc ccggccgatg gtccgccaga agtggggtag gatcgtcaac atcgcgtcgg
      421 tggttgggct gaccggaaac gcggggcagg ccaactactc cgcctcgaag gcgggcttac
      481 ttggcctcac gaaagcggtt gcgaaggaac tggcgtcgcg ccatattacc gtgaatgcgg
      541 tggctccagg cttcatcgaa acagccatga cggacgccct gggcgacaag gtccgggacg
      601 gcctcaaggc gcagatcccc atgggccgct tggggaaagc ggaggacgtc gcccaagccg
      661 tgggcttcct ctgctccgaa ggcgcggcct acgtcaccgg tcaggttctg accgtggacg
      721 gcggtatggt gatgtgatcg gggggcgttc cgaagccctg ccggaccggg acgaatccaa
      781 agggaggtga gcaaggacat ggctgcgttc agcgaagagc gtgtgaagca gatcatcgtc
      841 gatcagttgg gcgtcgcgcc cgagcaggtc accgcggaag cgtcgttcat cgacgatctg
      901 ggcgccgatt cactcgacac cgtcgagctc gtgatggccc tcgaggagga gttcgatctc
      961 gaaatccccg acgaggacgc cgagaagatg acgaccgtgg cagatgcgat caagtacctc
     1021 gaaggtcacg tgccgaagaa cgcctaattc ggcgccgcgg gtccggacga tccagacccg
     1081 cgcggagcag ggcggtttca cgaaagggaa cgaagaaaga gcatgagcca atcgaacgga
     1141 cagccgcgcc gcgtcgtcat cacgggcatg ggagtggtct ccccgctcgg gtccgacgtc
     1201 gaggtcttct ggaagcggct gaccgcgggc gaatccggca tcggccccat cacgcgattc
     1261 gacgtcacga agtacgacac gcgattcggc ggggaggtgc gggaattccg cgccgaggac
     1321 tacctcgaca agaaggacat ccgccgcgcc gacctcttcg tgcagtacgc gatcggggcg
     1381 accgcccagg ccgtgaagca ggcgggggtg tctcccgaga ccgtggaccc gaaccgctac
     1441 ggagtcgtgg tgggctcggg catcggcggg atcgccacat tcgaggacca gcatcggacc
     1501 ctgctcgaaa agggtccatc gcgggtgagc ccattcttca ttcccatgat gatctcggac
     1561 atggcgtcgg gccaggtgtc gattcaattc ggcgccaagg gaccgaacta ctgcacggtg
     1621 tcggcctgct cctcgggcgc gcacgccgtc ggcgacgcgt tccggatcat ccagtcgaac
     1681 gaggcggacg tcatgatctc ggggggggct gaggccccga tcgccccggt cgcgttcgcc
     1741 ggtttctgct ccatgaaggc gatgtccacg cgcaacgacg atcccctgaa agcttcgcgc
     1801 cccttcgacg cccagagaga cgggttcgtc atgggggagg gcgccggcat cgtggtgctc
     1861 gaagagctcg agcacgcgaa gcgacgcggc gtgaagattc tggcggagat cgtcggctac
     1921 ggcgcaacgg gagatgccta tcacatgacg gctccggccc ccgagggtga gggtgcgggc
     1981 cgtgccatga gggcggcgat ccgggattcg ggcctttcgc tcgagtcgtt cggatatctg
     2041 aacgcccacg gcacctctac gccactcaac gacaaattcg aaacacaggc catcaagtcg
     2101 gtcttcggag atcgtgcgtc caagctcccg gtcagctcga cgaaatccat gacgggacac
     2161 cttctgggtg ccgcgggagg gctcgagacg atcatttgcg tcctcgccct ggagcggcag
     2221 acgattcccc caacgatcaa ctacgagact ccggatcccg actgcgacct cgactacgtc
     2281 cccaacactg cacgagcggt cgaactgcag gcagcccttt ccaactcatt gggctttggc
     2341 gggcacaacg tcaccctggc gttgtcgagg tacatggcgt aggaggatcc cgaaagggat
     2401 ccgcaccacg tgaacttttt gcgaaagctg ttcggtctgg accagcccgc cgcccccaag
     2461 gcgccggcgc cggtccgctc gcgcgatcgg gacttcgatt tgaagggcct ggagcgcatt
     2521 ctcaaatacc agttcaaaga cgccggtctg gcgcgcaccg cgatgacgca ccgctcctat
     2581 ctccatgcga gcccggggcg gagcggcgaa tcgaacgagc gcatggaatt ccttggcgac
     2641 tcggtcgtgg gtctcgcggt gaacgagttc ctctacaaca aatttccgaa gcttcgcgag
     2701 ggcgagctga cgaagatgaa gtcgctcctc gtgagccgcg tgatcctgtc gcgcacggcg
     2761 aatctcatgg ggctcggaaa ctacatcctg ctctccgagg ccgagaacgg ctcgggcggg
     2821 cgcgggcgcg cgtccatcct cgccgacacc ctggaagggg tgatcggcgc ggtctacctg
     2881 gacgggggac tcgagccggc gaggaagctc acggagcggc tcctcctgcg cgaggtgcac
     2941 gagatcctga gcgacgcgaa tctcgcgaac tacaagagca tgctgcagga atacgtgcag
     3001 ggcgaattca agacgcaccc gcagtaccgg atctcgagcg agaacggccc cgaccaccag
     3061 aagatgttcg ccgtcgaagt ggtcgtgaac gggaagacgc tcggccgggg ccacgggaac
     3121 aacaagaagg aagcggagca ggaggcggcg cgcgacgcgc tccttcactt cgagaagctg
     3181 gtccgccccg atcgcggagg cgaggaggca ggctcggcag gacgccgccg ccgccgcgga
     3241 ggacgcggac ggcgcggagg ccggtcgggc gaggggcgga tggaggacgg ctcttcggga
     3301 ggcgaatcgc gtggacgcgg aggtcgcgaa ggcggccggg atcgcgaacc gcgtgaggga
     3361 cgggaaccgc gcgaagggcg ggaaccgcgc gaaggacgcg aaccgcgtga agggcgggag
     3421 gccggctcga gccgtgacgg gggtgaagcg cgtgaggggc gtgaaggccg ggagggtcgc
     3481 cgccagcgcg gaggccgcga ccggcgaggc cgcggatcgc gcgagccgca ggcaactcgg
     3541 gagccgcgcg aagaaacaac gcgggacgtc tatccgccgc aacgagaggt gaagcctcgc
     3601 gacaacgagc cctacgaggc tccggaaccg cgagtggagc gagagcccga ggtgccgcgc
     3661 gagcactcgg agccgcgtcc ctatgacgag ccgcgcgatc gaggcgaatc gttcgatcgt
     3721 ggatcatccg gcggatcgtc gagtgaaggg agtgagaacg gaggcgggtc gagcgagaag
     3781 aatttctctc cgaatcgtga tccctgggcg gcttcgcgct tgcacaccga ttcgtagacc
     3841 gttataatcc aaagcgtcgt ctcgagacgg gggaggaagg cccgataggg tcttcctccg
     3901 ttcctttttc gggagacgac ctgaccgtga acgtccagaa gcgggcagca ctagcggcac
     3961 tcaccttcac cctggcttta gctccctcgc ctgcccgcgc ctacttcttc tcgctctaca
     4021 cacccccaac cgtcacctgc cagatccagt ccgagctggc ggaggcagcc ggggaccacg
     4081 ccggggccat cgcctgggcg gagtcgctga cggttctgga tccccaatcg agctacgccg
     4141 cgtcgcgcgt ggcgagcctg tacgaggacg ggggcgagga cgagatggcc ctccactggg
     4201 gggaccgggc gctgtccctg gacagcctga acgtcgaggc cgccatgttg gttggccgca
     4261 tgcggttacg ctcgggagag cccgggctgg ccgtgcaggc cctgaccccg ccgctccgcc
     4321 tcgtgggagc cattcccgag ctctacgctc ttcgctcgct cgcccacgag ctcaaccgga
     4381 attacgaggc ggcgctcgcc gacctcaaac gaaccgatgt gctcctgccc gatttcgcgt
     4441 ggatcgcgac cgggatcctc agtctctcac tggaggacgg ccggctggag gaagcgtctt
     4501 cagcccttca tctggccctg gagctcaacc ctgacgactc gcgcacgctc cgcctgggcg
     4561 tggccctggc ccggcggaac ggcgatccgc tcctcgaggg gcggctcctc aaacagctgg
     4621 ccctagccga cgagcctcgc ccggaagacg tcgcgagcta cgccgccttc ctcatgcgct
     4681 cccgaaacga agccgagctg ggcgggctct tccagcgagc cgaagccaag ggcctgaacg
     4741 gcgacgacgt ccgcgtggac gccggtcagg agctcctccg cttcggcgac tacgcccacg
     4801 ccctcgaggc ggtgaagccg ctcaagaagg acacgcgcgc ggtgccgatc cgggcgcggg
     4861 cgtacctggc tctgggcgag gagaagaagg cgctcgaagg gtaccggcgg ctcctgccgg
     4921 gacggaccct ggtgcgtgag gagtcgctca tcgtggccta tctcgagatt cgagtcggcg
     4981 accggaacga ggggatccgg acgctggagg atacgcggtc gcggccgttc gacacgccgc
     5041 ggcaggtcct cgccgcgagc ctgtgctact ctctacttgg ccacccggag gaggctctga
     5101 gcctggtccg cgagagcgcg gcccggggaa tcgacagccc ctccatctac gaggagctgg
     5161 gctcggcggc gacggtgctg ggggacagcc tcctcgcgca atgggcttgg gagcgtctcc
     5221 gggacatggg ccaggagacc tcggaatgcc tcttcttcct cgccagctcc gagctggggc
     5281 agggggacca agccaaaggg atggccaccc tcacccgctc gatccagctc gaccccaaga
     5341 acggacgggc gctcctcctc atggggaagc tccgccacag gctcgggcag ctcgagatgg
     5401 cgcgcgagat cctcatccgc tccgcccgct gtccggagac ggccgccgag gcaaatcgag
     5461 agctggccaa ggtttgccgg actctgcgcc tggactcgga agcccgggac gcggagagtc
     5521 gcgcccgctc ggggcgcccc gcgccgccgg ccggcctgtc cctcttccag aacccctgac
     5581 ctttccggga gcgcttcgga actctgctcc ccgccacggt gtagcatgag cacggagaac
     5641 gacgacgtgc ccattgagcc cctacgcctg aaaaagctcg acctcgccga gctgaccgac
     5701 gaagacgtca tggagcgctg tgcgatgggc tccgaggcgg ctttccgcac gctcgtccag
     5761 cggtatcgca cgcggatcat gaatctggtc tgccgcttca tcaacgaccg ggatcgggcc
     5821 gaggagatca gccaggaggt cttccttcgg gtcttccgga accgcgagcg ctaccggaag
     5881 agcgggaagt tctccacgtg gatcttcacc atcgcggtga atctcacgaa gaacgagatc
     5941 cgtagccgcg tccgtcatcg cggcacgttc agtctggatg caatggagga ggagtcgagt
     6001 ggccagggcg tgacgtttcc cgacgccaaa ccgctccccg acgaagacct gaatgcccac
     6061 gagatcgggg ccaaggtcgc cgaggcgctc cgcaaaatcc ccgcgcgtta tcgcgaggcg
     6121 gtggtgcttc gagatgtcga ggggctcagc tacgaggagg tgggtcagat cctgcgcatt
     6181 ccgggcggca cggttcgctc gcgcatcaac cgcgcccggc tcatgctgaa ggagagactc
     6241 aaaccgtacc tcaccctgga ggatgtatga actgccggtc cgccgaatcc cttttttcag
     6301 ccttcgtaga ggacgagctc aatcagaaag agcgccgctt cctcgagtcg catctcctct
     6361 cctgccgccg ctgcacggcc tcgatgcgcg agctcaaggc ctcgatggaa ctctgcgccg
     6421 cgctcccgga cgtggacacg agcccccact tcgaagacga cgtgatggcc atgatccgca
     6481 acggtgaggc tctgcgccct tcgctcatcg agtggctgcg cggcctcgtc acgatcgagc
     6541 gcatccgtcc gttcgcgctc gccggtgcgg gcgcgtcggc ggttctgatc ggctcgttcg
     6601 tgatcaatac gctccacctg aactcatcgg ctgtggtcgc ggaccgtacg accaaggcca
     6661 accagaccca gcccgccacg ccgtccggcg tcccgactca ggaagaggtc gccgccacga
     6721 cgatgcccga ggcgacggct cccgcgccga gcgcgccggc tgccgtgaag agcgacgcgg
     6781 gctatgcctg ggccggtcac agcgtccgga cgtcgagcat tccggacacg acggttccca
     6841 atccgggcgc ccgctacgaa gacgagtaca tcaccgacca gttcatcctc gagcgcggga
     6901 cggacggagg caacaacccg accatcaccc ctgtctcgag tcgcgcgtcg gatgacgtct
     6961 acatcacgtt ctagcagctc gatgtcccag ccccggcaca agcggggcgc cgccgtcctc
     7021 gcgttctcgg tcctggtcct cgcgggaccg ggcgccggcg tctcccatgc ggttcctcgc
     7081 cacgtcgcct tccgctccga cgaagcctcg ctcctcgggg tcgtcgactc cgttcacgcc
     7141 tcgatcgtga ccgtggtcgg ctatcccccg cccgatccca gcgggcacgc cggcaagcgc
     7201 cgccggttga tcggatccgc cgtcgccatg gctccggatc gcatcatcgc ctcggcgagc
     7261 gtcgccattc ccggcggcag tgtgcgcgtg ctcctcggaa acggcgtcga gcgtcccgcg
     7321 atcttgaggg gcgtggatcg ccagtcgaac atcgcgctct tccaggtgga aggagcccag
     7381 ctcgagccgc tccacgcggc cccgccgcag tcgccggccg tgggatcgtg ggtcgcggtg
     7441 gtctcgaacg tcggcatcac gcgcccgcag gcggccatcg gccagatcgt cggccgcggc
     7501 gagcgggtgg atgagcacac ggggggcgac atcttcgaga tcgacgctcc cacgtatccc
     7561 ggctcgaccg gcgctgcgat cctgaacgag gatggggagt ggatcgcgat ggtcgtcgga
     7621 cgagccgccc cgagcaccag cgacggctcg ccggtcggtc tggattcggg ggagtcgatg
     7681 ccggggccga gcagcgtgct gatcgcgctg ccggtggacc aggtgatgta catcacgaag
     7741 gagctcgaca cctacggcag cgtgcggcgc ggcttcctgg gcgtccagtt gaagcgcggg
     7801 cccgcgagcc cctccgactc gctgggcatc ctggtcgcca acgtggtgcc ggggagtccc
     7861 gccgactcga gcggactcag ggtgggggac cggatcctgg cggtcgatgg ccaggaggcg
     7921 cacacggcgg acgatctcac cgccatcgtg cgcgcgatgc gtccggggga cgacgtggag
     7981 ctgaccgtgc tccgcaagga tgacatcttc ccgatccggg tcgtgctcgg cgccgcgttc
     8041 accaatccgc cggtcggacc acgcccctcg cgcgaggagg agatccgtcg cgcgaagacg
     8101 gatctcaacc ggctcgagat ggagaagcgc cagctcgagc agcgtctccg caccctcgag
     8161 ggctcctcct cccgctgacc cggcgccgga ttcccgcgcc ttggtccacc gcgagtcgac
     8221 cccccttctt ctcgctccgc cgcgcgtgtg ctagcctgcg cgccacgatg cggcgtcctc
     8281 gaacgctccc catccatctc tcgattcgcg agatcccggc cgccgcggac tcgatcggca
     8341 cgccatgact cgaatccgcg tccgcccgaa gcgggttgcg tacgacgtga ccgtcgccgc
     8401 gggcatcctc gcccgggcag ggtcgctcct tcgccgaacg caccgcggct ccacggctgt
     8461 cgtcgtcacg gatcgcacgg tggaccgact ccacggtgcg cggctcgttc gctcgctcac
     8521 gagcgcgggc ttcatcgtgc ggcgcgacgt gctgccggac ggcgagcgcg cgaagtcggt
     8581 cgcgacgctc gagcggctct gggatcggtg gtcgcggagc ggcctctcgc gcgacgcgat
     8641 cgtcgtcgcg ctcggcggag gcgtcgtctc ggacgtcgcc gggttcgcgg cggcgtgcta
     8701 tgcgcgcggt cttccgtggg cggccttccc gacgaccgtg atcggacagg cggacgcggc
     8761 catcggcggg aagacgggcg tcaatctcgc gcttgggaag aacctggtcg gcgcgttcca
     8821 tcatccggtc gccgtctacg cggacgtgga cacgctgcgc acgctcccga cgcgcgcgta
     8881 tcgcgcgggg ctggccgaga tcgcgaagat cggcgtgatc gcccgccccg gcatcctccc
     8941 caaactcgag cgcctcgctc gcgcggggac gcgcgccgcg cgtcccacgc tcgtggcccc
     9001 gctcgtgcgc tccgcggccg aggcgaaggc gacgctcgtc gaacgggacg agcgcgatca
     9061 gggcgcgcgg ctcgcgctca acttcgggca cacggtgggg catgctctcg aagcggcgac
     9121 cggctaccgc cggtatctgc acggggaagc ggtcgcgatc ggcatggcgg ccgcgctccg
     9181 gatgagtgtt ctcgaggcgg gcctcgatcc cgtcgacgcg atggaagcgg agtcgctcct
     9241 cacggcgctc ggactcccga cgcgcctctc gcgcgagccg ggccgcgcgt tctggatggc
     9301 cctggctcga gacaagaagc gtggacgatc cggcgtgcgg atggtattgt gccccgcgat
     9361 cggccaatca aaagtctttg aattgccagc gcttaccacc cttcgacggg tcgttcgaag
     9421 cttggtccgt catccgggag ggaacccatg aggggcgacg cgcccaagga gacggcgctg
     9481 ccggtcgagg agatcatcgc caaggatcct tcctccgcgc tctacctgcc ctatgcggag
     9541 cggctggtcg aagacggccg gctcgaggag gcgatcgcgc tctgcgagga gcgcaagcgc
     9601 cgtccggggc gaggcgtggg tgatcacatc gtcctgggcc gggcctatct cgccgacgga
     9661 cagcttggcc ccgcgcgcgc cgaattcaag gaggcgctcg atctcgaccg cgagaacgtc
     9721 gtcgccctga aggcgctcgg cggcatcctc gcgcacgagg gaaaccacac cgaggccgcg
     9781 agctactacc aggcggtctg ccgcgtggac ccgggcgatc tcgagtcgca gaccgcgctc
     9841 catcagatca cctcgggcga atatccggag atccgtcccg ccgacgtgat cgtggggcag
     9901 ggtgccatga gctggcagcc ggtccgcctc ccgcgcgagg aggagcatct ctccgagctc
     9961 gcgctcgggc tccgcacgat cgagcggttc gatgcgtcgc agacggagcg gacggccccg
    10021 cgtccgtaca cggctcccga aggggatttc gatctcccgg tcgagcggaa cgcaccggag
    10081 gaagcgcgag cggcgatcga gccgcgggat ctaaccgcgc cgatcgcgga gcatctcgcg
    10141 ctctccgaca tggccgcgga cgctgcgtcg gagtcggatg ccgacgagtt aagcccggag
    10201 cccgcgccgg cgcacgcgga agcgcacgtc gcggccgggc cgtccggcct cggagagatc
    10261 gtcgagagcc aaccgccgat cgaggtcccg cgcgctccgg ggaaggtcgt cgaagggaac
    10321 aagaacgcgt tcgagacctg gatccgtcag ctgggcggaa aggagtagcg agatcgcgcg
    10381 cgacgtctgg gtgctccacg gtcccaatct ggggctgctc ggccggcggg agccgaatct
    10441 ctacggcaag gagacgctcg cttccatcga ccggcgcctc gtggagctgg gcgaggccct
    10501 cgatctccgc gtctcgtcgt tccagaccaa ccacgaaggc gcgctcgtcg accgcttgac
    10561 ggtcgcgatg gacgaagtcg acggctgcct cctcaatccg gccgcgttca gccacggcag
    10621 cctcgctctg gccgacacgc tccggtcgat gaccatcccc gtggtggagg tccacatgac
    10681 caacctgtac gcgcgcgagc gcgaacggca gttcagtctg accggcagcg ctgtcacggg
    10741 cgtgatcatg gggttcggcc ccgcgagcta cgagttgggg cttcacgcgc tcgccgccat
    10801 gatcgcgccc gcgagggggc ggaggaagcg gacgtgagcc tgaagcgcga catccaggag
    10861 ctggtccaga tcatgcgcga ggaagacctc gccgagatcg aggtgcgccg gtggttctcc
    10921 tcggtgcgcg tgcgtcgtcc gatgagcgag caggccctgg tccacgcgcc cgccatggcg
    10981 ccggcggctc cggcgtccgc gcgcgaggcg gaggcctccg cggaatccca gctcacgccg
    11041 atcaagtccc cgatggtggg gaccttctac cgctcccccg cgcccgacgc ggatccgtac
    11101 gtcgaggaga actcgtcggt cgacgtgggg cagacggttt gcatcgtgga agcgatgaag
    11161 ctcatgaacg agatcgagtc ggacgtgcga ggacgcatcg cgaagatcct cgtcgagaac
    11221 gcgcagccgg tggagttcgg gcaggtcctg ttcctggtcg aggcggagag cgccgcgtag
    11281 gcgcggaggg cggggaaacg gcatgaatat caagcgcgtg ctgcagcgga tggtcgagat
    11341 gggagcgtcg gacctccacg tcaaggtggg cacgaagccg accgcgcgcg tgaacggcga
    11401 gctgatcccg atcgacgaag cgccgccgag ccaggcggat ctcgagacgg tcgtggacca
    11461 gatcctcacg ccgaagcaga agcgggtctt cgaggagacg aaggaggtcg acttcgcctt
    11521 cggcgtcacc ggcctctccc gcttccgcac gaacttctac cagcagcgcg gcacgttcgc
    11581 gatggtcttc aggcaggtgc ccgcgacgat ccccgccttc gaggatctga accttccgtc
    11641 cgtgatcgag gacctggcgc tcaagccgcg cggcctggtc ctcgtgtgcg gcaccgttgg
    11701 gagcgggaag tcgacgaccc tggccgccat gatcgactcc ttgaaccggc gcgagccgcg
    11761 gaacgtcatc acgatcgagg atccggtcga gttcctcttc cgcgacaaga agtgcacgat
    11821 ctcccagcgc gaggtcgggc tcgacacgga cagcttcggc gaggggctga agcacatcct
    11881 ccgccaggac ccggacgcga tcctgatcgg cgagatccgg gatccggtga ccatgcgcac
    11941 cgccctcatg gcggccgaca cgggacacct cgtcctctcg acgctccaca cgatcgacgc
    12001 ggcgcagacg gtgaaccgca tcatctcctt cttcccgccg cacgagcacg aggaagtccg
    12061 ctacatgctc tcgaccacgc tccaggcggt ggtcgggctg cggctcatcc cgcgcgccga
    12121 ccagtcgggg cgaattccgg cggccgaggt cctcatcgtc acggcgacgg tgcgcgagta
    12181 cctgctcgac cccgagaaga cgagcctcat caagacggcc atcgccgacg gcgtgtcgca
    12241 gtacggaatg cagaccttcg accagtcgat catgcggcac tactcggaag ggaagatctc
    12301 gatcgagaac gcgctcaagt actgctccaa ccccaccgag ttcgagctcc gcgtgaaggg
    12361 gatccacgcc acctcggacg agacctggaa gacgttcgag cgtcccaagc aggaaaccct
    12421 ctagggcgca tgttccggaa gatcctgatc gccaaccggg gagagatcgc gctccggatc
    12481 gttcgcgccg cgcgcgagat gggcatcgag acggtggccg tctactcgga ggcggaccgg
    12541 gactcgctcc acgtgcggct cgcggacgaa gcggtctgca tcggcccgcc gccgagctcc
    12601 gcgagctatc tgaacatggc gcgcatcatc agcgccgccg agatcaccgc cgccgatgcc
    12661 atccaccccg gctacggctt cctctccgaa aacgcccact tcgccgaggt ctgtgagtcg
    12721 tgccacatcc gcttcatcgg cccgacctcc gagatgatcc gcacgatggg ggacaaggcg
    12781 gtggcccgga agacggcggt cgcggcgggc gttcccgtca cgcccgggag cgaaggggtt
    12841 ctcgagtctc ctgaccaggc ggccgacgtg gcgaagtcgc tcggctatcc cgtcatcatc
    12901 aaggcggcgg ccggcggcgg cggcaagggg atgcgcatcg cgaccgacga agcctcgctt
    12961 aggaacggcg tcatgatggc gcaggccgag gcggaggcca acttcggctc cggcgccgtc
    13021 tacctcgagc gctacgtcga gaagccgcgg cacatcgagt tccaggtgtt cggcgacaag
    13081 caggggaacg tggttcacct gggcgagcgc gagtgctcca tccagaggcg ccaccagaag
    13141 ctcatcgagg aggcgccgag cgtggcgctc tcgccggagc tccgcgcgcg catgggcgag
    13201 gcggcggtgc gcgcggcctc gtccatccgc tacgaggggg cgggcacgat cgagttcctg
    13261 ctcgggccca cgggcgagtt ctatttcatg gagatgaaca cgcgcatcca ggtggagcac
    13321 ccggtcacgg agatggtcac ccggcacgac ctcatcaagg ctcagatcct ggtggccgcc
    13381 ggcgaacccc tcccgtggaa gcagagcgag atccagctcc atggccacgc gatcgagtgc
    13441 cgcatcaacg ccgagcgccc cgaccagaac ttccagccct cgccgggcac cgtgcgctac
    13501 ttccacagcc ccggcggacc cggcgtgcgc gtggacagcc atctctactc gggctaccgc
    13561 gtgcctccca actacgactc gatggtcgcg aagatcatct gctggggcca gaatcggagc
    13621 gaggcgatcg cgcggatgcg ccgcgccctt ctcgagaccg tggtcgaggg aatcgacacg
    13681 acgctgtcct ttcacctcga agtgctcgcc gatcccggat tccagaaggg cgagatccac
    13741 acgggctacc tggaagagtt catgagccag aggaccaagg cggcgtgacc tcgacgggcg
    13801 cccggggcgc ctcgggcatc gcgcggatcg acctctacct cacgccctcc gaagtcgagc
    13861 gcgcgccgct cgaggatcgc cgtctcgtgg tcgtggacgt gctccggagc tccacctcga
    13921 tcgcgacggc cctccagaac ggtgcctcga agatcatccc ggtcgagacc gtggaggcgg
    13981 cgacccgcct cgcgccgacg ctcgacccga agtcgcggct cctctgcggt gagcgcgacg
    14041 ggcggaaggt gggcggcttc gacctcggaa actccccgcg cgagtacacg cgcgagcgcg
    14101 tggaaggcgc gacgctggtc ttcgcctcca cgaacgcgag tccgctcatg gcgggcccac
    14161 tcgacgggcg cgagcagtgg atcctcggct acatcaatct gggggcgatg gcggagctca
    14221 cgcgatccga gggggcggac ctcgccattc tctgcgctgg gaagagcggc cgcttctcgc
    14281 tcgaggacgc ggcgtgcgcg ggcgcgttga tccggcggct cgccggcgac ggcgattcga
    14341 tcgtgttgaa cgacgccgcg gagatggcgc gcgagtacga ccggcgccac ggcgcggatc
    14401 ccgagggcat cctccgcctg agcgagcacg gccgctactt gatcgagctg ggattcgagg
    14461 aggatctgcc cgtctgcgcc cggatcgaca gcgtgccgct cgtgcccctc ctcaaggacg
    14521 gacggatcac ggcaggagcg gcccccgcct cgcgccgcta gccgccccgc ctcgcgcggc
    14581 gcaggagctc gagcgccacc accccgaaga agagattccc gagccaggcc gcaagcatcg
    14641 gcgggagcgt gccgttgtgt ccgagcgact gcccggtccg gatgaaggcg tagtagacga
    14701 agctgatgaa gatcgagagc ccgaagctga tcgcgaatcc gccccgccgg acgcgcaggc
    14761 cgagcgcggt gccgatcacg accacgatca ggttcgtgag cgggaacgcc accttgaggt
    14821 agagctccac caggtatttc tgcacgcggc tcccgctctg cttgagcttt ccgatgtact
    14881 tctcgagctg ccagtaggag agcgccatcg gatcctcctc ggccttcgcg aagtcctcgg
    14941 gcttctcgag caggccggga atgaccaatt cttggaattg ggcggcgtgc
//
LOCUS       DASTKC010000164         5414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1311164, whole genome shotgun sequence.
ACCESSION   DASTKC010000164 DASTKC010000000
VERSION     DASTKC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311164"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VFR25_06475"
     CDS             <1..1110
                     /locus_tag="VFR25_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940728.1"
                     /translation="VVKGLERTPASGVPVSLHPLRNEEELPGQTVASGPDGTFRFTGI
                     TQDPSTSYFLSTEYEGAFYTEGPVEFKGETGAKDIQIFDVGRDMASVRVTNHHILVER
                     AEDHLHVTEILVFQNDGSTAYLGTGLNHAENVGVRLGLPASVKKFEPGMGGDEGSVVL
                     QGRDLASHRPIPPGQRPFSFSYEIPLSGRVDLSHRIYFPTESVDILINDPQLKVEGSS
                     LTYTGSRDQGGKKFEIYQAKNLPVGSELTMRVHGASFWSNPKIYPWLVAPFVIVAVLI
                     FASRRGRRGDAPAPRTAPEPRDAPAAVAAPKVASLAPDSNGDDDLHTVYLYLIDALDK
                     GLAQGEFTRESHDLVRGNLKRRLETILSDRTPTSR"
     gene            1115..1753
                     /locus_tag="VFR25_06480"
     CDS             1115..1753
                     /locus_tag="VFR25_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940729.1"
                     /translation="MILRAEAVTKRFDDRPVLSRASLTLEPGQVVHLAGPNGSGKTTL
                     ARILGTLLPPDEGEILVDDVPLSERRSQTRRLIGFASHAPLLYPSLTPVENLEFFAQL
                     AGARDGRRRALALLERFGLEQAADRPLAHFSRGMLQRVVLCRALLLDPELLILDEPYA
                     GLDDAGILVVNQVLSEIRKQRRTALLIAHDKERGGGVITRMVRLRNGRVEDA"
     gene            1750..2421
                     /locus_tag="VFR25_06485"
     CDS             1750..2421
                     /locus_tag="VFR25_06485"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET9940730.1"
                     /translation="MTFLLVMKKDLRLFVRDARFLATLGLFSASLVLILSVAFGPIFT
                     EPPRLAAATVWAALFFAGVLGLTRSLELEGESGAGDALRLSGADAFSLYLGKTLANFA
                     LLAAVFILLLPLTAIFFDVGTLKALPGVLGVAALALIGYSAWGTLFAALARGLQAREL
                     LLSVLLFPSLVPLWIGAVKLTHAYWGGVPVDEVKDWLKVMAVMDVLGLALAAWLYEAV
                     QEAGE"
     gene            2418..3122
                     /locus_tag="VFR25_06490"
     CDS             2418..3122
                     /locus_tag="VFR25_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HET9940731.1"
                     /translation="MTRDVPLWWRNGARWVWILAAALVVASLWMVTRVPVERIMGPVQ
                     KIVYIHVPSAIVTLLAFGVTFAAGIAYLATKQWIWDAIGAASCEVGLVFASVVMITGP
                     LWARSAWNTWWTWEPRLTTFLILWILYAGYQIIRSSLKSGSKRTISAIMGIVFFVNLP
                     IVWKSVEWWRGALHPKNVTMAPDMRQVLLFGMLAWFVVYVAVFERRLRLEWAREAATR
                     EAHATADSTLLEARVR"
     gene            3231..3566
                     /locus_tag="VFR25_06495"
     CDS             3231..3566
                     /locus_tag="VFR25_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9940732.1"
                     /translation="MRDHFLEFEISPELVHEMIDTGEDPILLDVRDDWEWEHAHLEGA
                     IHIPMDELAGRIVELDPRSEIIVYCHHGDRSIDGCLLLWEHGFRKVRSLTGGIEAWSE
                     LIDPTVPRY"
     gene            complement(3579..4739)
                     /locus_tag="VFR25_06500"
     CDS             complement(3579..4739)
                     /locus_tag="VFR25_06500"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET9940733.1"
                     /translation="MSRDVGRPAAAARWIVAAAAALLLGGAAKAPFDPMNPRDATTFP
                     RAHGAHPGSPLEWWYLTGRLEDGDGRSMGFQVTFFRARIDAANLGAAPVPSAWRPGDV
                     YFAHYAISDLKARFYRFDERIGRTSVALAGADSTDLDVRIRDWSLRRLPSGVFRVHAA
                     GDLGDLALDLTPSRSTPVAWGPQYFSAKNAAGTSFSRYHSDPRMRAQGTYTAPGAPAR
                     RMRGTVWYDHEWSGGAYDSTLKGWDWFGLRLQDGRSVMLYRMRDRQGGTSYLFGGIVD
                     RGGRVRTFTREDVTMNALRYWTSPKTGARYPLSWDITIRPTGEAPLRLTVAAALSNQE
                     LTTERSTRVAYWEGCVEGKEEEADPDQRVEGYMELTGYAGGGVPGRVSNVGR"
     gene            complement(4732..>5414)
                     /locus_tag="VFR25_06505"
     CDS             complement(4732..>5414)
                     /locus_tag="VFR25_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9940734.1"
                     /translation="LLIPTPTGSHVYQVMAVYRDYSSDRGYVILDRSIYLQDFRDSLV
                     TTYALYLAPGVSRERGARAAEAALGQDEVIRVTDTRSIREEVRRVMRNTFAVTDALEA
                     VAAMVALLSVLGALAALVLERTREIGVLRAIGADRSQIMRALLVEGGLLAVTGLVLGA
                     LTGVLLSAVLVHVLNRESFGWTLELSTPWVRVLLVAAVLFAAALLATLPPARRAARIP
                     PREAMNRV"
BASE COUNT          805 a         1878 c         1856 g          875 t
ORIGIN      
        1 gtggtgaaag gcctggagcg gacgccggcc tccggcgttc cggtctcgct ccatccgttg
       61 cgcaacgagg aggagctgcc cggacagacg gtcgcctcgg gccccgacgg aaccttccgc
      121 ttcacgggga tcacccagga tccctcgacg tcctacttcc tctcgaccga gtacgaaggc
      181 gcgttctaca cggaagggcc cgtcgaattc aagggcgaga ccggcgccaa ggacatccag
      241 atcttcgacg tcgggcgcga catggcctcc gtccgggtca cgaatcatca catcctcgtg
      301 gagcgcgccg aggatcatct ccacgtcacc gagatcctgg tcttccagaa cgacggctcg
      361 accgcctacc tcggcacggg gctgaatcac gcggagaacg tgggcgtgag gctcggcctg
      421 ccggcttccg tgaagaagtt cgagccgggc atgggcggcg acgaaggctc cgtcgtgctg
      481 caggggcgcg acctcgcgag ccatcgtccc attcccccgg ggcagcgccc gttcagcttc
      541 agctacgaga tcccgctctc cggccgcgtg gacctctctc accgcatcta cttcccgacc
      601 gagagcgtcg acatcctgat caacgacccc cagctcaagg tggaggggag ctcgctgacc
      661 tacaccggct cgcgcgacca gggcgggaag aagttcgaga tctatcaggc gaagaaccta
      721 ccggtcggat ccgagctcac gatgcgcgtc catggcgcga gcttctggtc caatccgaag
      781 atctatccgt ggctcgtggc gccgttcgtg atcgtcgcgg tgctgatctt cgcgtcgcgc
      841 cgcgggcgga ggggcgatgc gcccgcgccc aggaccgcgc ccgagccgcg cgatgcgccc
      901 gcggccgttg ccgctccgaa ggtcgcgtcg ctcgcgcccg attcgaacgg cgacgacgac
      961 ctgcacacgg tctacctcta cctcatcgac gcactcgaca agggcctcgc ccagggcgag
     1021 ttcacgcgcg aaagccacga tctcgtccgg gggaatctga agcgccgcct cgagacgatc
     1081 ctctcggacc gcaccccgac ctcacgctga cgccttgatc ctgagggccg aagccgtcac
     1141 gaagcgcttc gacgaccggc ccgtcctgtc ccgcgcctcg ctcacgctcg agccggggca
     1201 ggtcgtgcat ctcgcgggac ccaacggatc ggggaagacg acgctcgcgc ggattctcgg
     1261 gacgcttctt ccgccggacg aaggcgagat cctcgtcgac gacgttccgc tctccgagcg
     1321 ccgctcgcaa acgcgccgcc tgatcggatt cgcgagccac gccccgctcc tctatccttc
     1381 gctcactccg gtcgagaatc tcgagttctt cgcgcagctc gcgggcgctc gcgacggccg
     1441 ccgccgcgcg ctcgcgctcc tcgagcgttt cgggctcgag caggcggcgg accggccact
     1501 cgcccacttc tcgcgcggga tgctccagcg ggtcgttctc tgccgcgcgc tcctcctcga
     1561 cccggagctc ctgatcctgg acgagccgta cgcggggctc gacgacgcgg gcatcctcgt
     1621 cgtgaaccag gtgctctccg agatccggaa gcagcggcgg accgcgctcc tgatcgcgca
     1681 cgacaaggag cgcgggggcg gcgtgatcac gcgcatggtg cggctccgga acggccgcgt
     1741 ggaggacgcg tgacgttcct cctcgtgatg aagaaggatc tccggctctt cgtgcgggac
     1801 gcccgattcc tcgcgacgct cggactcttc agcgcgtcgc tcgttctcat tctgagcgtc
     1861 gccttcggtc cgatcttcac cgagcccccg aggctcgcgg ccgcgacggt ctgggccgcg
     1921 ctcttcttcg cgggcgttct ggggctcacg cgctcgctcg agctggaggg cgaatcgggg
     1981 gcgggggacg cgctccggct gagcggcgcc gatgcgttct cgctctacct ggggaagacg
     2041 ctcgccaact tcgcgctctt ggccgcggtc ttcatcctcc tccttccgct cacggcgatc
     2101 ttcttcgacg tggggacgct caaggcgctt ccgggggtgc tcggcgttgc cgccctcgcg
     2161 ctcatcggat atagtgcgtg gggaacgctg ttcgccgccc tggcccgggg acttcaggcc
     2221 cgcgagctcc tcctctcggt tctcctgttt ccttccctcg ttcccctttg gatcggtgcg
     2281 gtgaagctga cccacgccta ctggggcgga gtgccggtgg acgaagtgaa ggactggctc
     2341 aaggtgatgg cggtgatgga cgtgctgggc ctcgccctcg cggcgtggct ctacgaagcg
     2401 gttcaggagg ccggcgaatg acgcgagacg tgcccctctg gtggcggaac ggcgcgcgct
     2461 gggtctggat cctggcggcc gcgctcgtcg tggcgtcctt gtggatggtc acgcgggtgc
     2521 cggtcgagcg gatcatggga ccggtccaga agatcgtcta catccacgtc ccgagcgcca
     2581 tcgtcacgct gctcgcgttc ggcgtcacgt tcgcggccgg gatcgcctat ctcgcgacca
     2641 agcagtggat ctgggacgcg atcggcgcgg cctcgtgcga ggtcgggctg gtgttcgcgt
     2701 cggtcgtcat gatcacgggg ccgctctggg cgcgctcggc ctggaacacg tggtggacct
     2761 gggagccgag gctcacgacg ttcctcatcc tctggatcct ctacgccggc taccagatca
     2821 ttcgaagctc gctcaagtcg gggtcgaagc gcacgatctc ggcgatcatg gggatcgtct
     2881 tcttcgtgaa tctaccgatc gtctggaagt cggtcgaatg gtggcgcggc gcgctccatc
     2941 ccaagaacgt caccatggca cccgacatgc gccaggtgct gttgttcggg atgctcgcct
     3001 ggttcgtcgt gtacgtggcg gtgttcgagc gtcgactcag gctcgagtgg gcccgcgagg
     3061 ccgcaacgcg cgaggcgcac gccacggccg attccacctt gctcgaggct cgcgttcgat
     3121 gacctacgcc atcatcgcct acgctttgag tcttctcctc tggctcgtct acgtgttcgc
     3181 gctcgggcgg cggctccggg ccgcgcgcga tcggcgctga ggaattgccc gtgcgggatc
     3241 acttcctgga attcgagatc tcgccggagc tggtgcacga gatgatcgac acgggggagg
     3301 accccatcct cctcgacgtg cgggacgact gggaatggga gcacgcgcat ctcgaaggcg
     3361 cgatccacat cccgatggac gagctcgcgg gaaggatcgt ggagctggat cccaggagcg
     3421 agatcatcgt ctactgccac cacggcgacc ggagcatcga cggctgcctg ctcctctggg
     3481 agcatggatt ccgcaaggta cggagcttga cgggcgggat cgaggcctgg tcggagctga
     3541 tcgacccgac ggtgccgagg tactagaact cgtcgcgctc accgtcccac gttcgacacg
     3601 cgccccggaa ccccgcctcc cgcgtagccg gtcagctcca tgtagccctc gactctctga
     3661 tcgggatccg cctcttcttc cttcccctcg acgcacccct cccagtaggc cacgcgcgtg
     3721 cttcgctcgg tcgtgagctc ctggttgctg agcgcggccg cgaccgtgag ccgtagcggg
     3781 gcctcgccgg tcggccggat ggtgatgtcc caggaaagcg ggtagcgggc acccgtcttc
     3841 gggctcgtcc agtatcggag cgcgttcatg gtgacgtcct cgcgagtgaa ggtgcgcact
     3901 cgacccccac ggtccacgat cccgccgaag agatacgacg tcccgccctg gcggtcccgc
     3961 atccggtaga gcatcacgga gcggccgtcc tgcagccgga ggccgaacca gtcccagccc
     4021 ttgagcgtcg agtcgtacgc gccgccgctc cactcgtggt cgtaccagac ggtgccgcgc
     4081 atgcggcgcg cgggcgcacc gggcgcggtg taggtgccct gcgcgcgcat ccgtgggtcg
     4141 ctgtggtagc gggagaagga ggtgcccgcc gcattcttcg ccgagaagta ctggggcccc
     4201 cacgcgaccg gcgtgctccg gctcggcgtg agatcgagcg cgagatctcc cagatcgccc
     4261 gcggcgtgca cgcggaagac gccggagggg agccggcgca gcgaccagtc ccgaatccgg
     4321 acgtcgaggt cggtgctgtc ggctcccgcg agcgccaccg acgtgcggcc gatccgttcg
     4381 tcgaagcggt agaagcgagc cttcaggtcc gagatcgcgt agtgggcgaa gtagacgtcg
     4441 cccggccgcc atgcgctcgg aaccggcgcg gctccaaggt tcgccgcatc gatgcgcgcg
     4501 cggaagaagg tgacctggaa gcccatgctc cggccgtcgc cgtcctcgag gcgcccggtc
     4561 agataccacc actcgagcgg cgagcccgga tgcgcgccgt gcgcgcgcgg gaaggtcgtg
     4621 gcgtcgcgcg gattcatcgg atcgaacggg gccttggcgg cgccgccgag caggagcgcg
     4681 gccgcggcgg ccacgatcca gcgcgcggcg gccgcggggc gcccgacgtc cctagacacg
     4741 gttcatcgcc tcgcgcggcg ggattcgcgc ggcgcggcgg gccgggggca gcgtcgcgag
     4801 cagcgccgcc gcgaagagaa ccgccgccac gaggagcacc cgcacccacg gcgtcgagag
     4861 ctcgagcgtc cagccgaagg actcgcggtt gagcacgtgg acgagaacgg cggagaggag
     4921 caccccggtc agcgcgccga ggacgagccc ggtcacggcg aggagcccgc cttccacgag
     4981 gagggcgcgc atgatctgac tccggtccgc accgatcgcc cggaggaccc cgatctcgcg
     5041 cgtgcgctcg agcacgagcg ccgcgagcgc gcccaagacc gagaggagcg cgaccatggc
     5101 cgcgaccgcc tcgagcgcgt ccgtcaccgc gaacgtgttc cgcatcaccc gccgcacctc
     5161 ctcccggatc gagcgggtgt cggtgacgcg aatcacctca tcctgtccca gcgcggcttc
     5221 cgcggcgcgc gcgccccgct cgcgcgagac gccgggagcg agatagagcg cgtaggtggt
     5281 cacgagcgaa tcgcggaagt cctggagata gatggaccgg tcgaggatca cgtagccgcg
     5341 atcggacgag tagtcgcggt agacggccat cacctggtac acgtgcgagc cggtcggcgt
     5401 ggggatgagg agcg
//
LOCUS       DASTKC010000165        31826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_838518, whole genome shotgun sequence.
ACCESSION   DASTKC010000165 DASTKC010000000
VERSION     DASTKC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 31826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31826
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838518"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFR25_06510"
     CDS             complement(<1..826)
                     /locus_tag="VFR25_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940735.1"
                     /translation="MRARDRDVKRIVMLILALAAAILFTPAGRACASDQAGPSADPRF
                     ALHVEGVADSVIAGETGETAPLERLWQRTEIERLAGRLRDRLALLGRYDAVVRLTLEE
                     GSGTTPGTATIRVEGTAPAPPAPPLAPALRLAPPGAVGIHDPAGVFARGAKGGVSPQA
                     IAGGLDALRDAAVDLGRYAATVSLDSIVPAGDSVRVHVRLSTGPPVVFDSLEIPGASV
                     TKPRSAATIAGLREGRVLTPELVTEARDRLRASDLFAFVGEPRVVAGSEPGHGHVVI"
     gene            complement(826..4107)
                     /locus_tag="VFR25_06515"
     CDS             complement(826..4107)
                     /locus_tag="VFR25_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940736.1"
                     /translation="MIESRFGSRIVMGASIGAALAWGLGPLPANARPIDSLCPGGCDA
                     PRDTVVIELPVARAPSLLALRASGPLAAGAADQAPPDSTASVLRNETRSWIAAAPAAG
                     AAGGTIADAASNDAITFSGLKLVSVEVGANRAASMTQSLDLTIRGRIAGDVQVAASLS
                     DRRLPFEPDGSSRELEDLDNITLSLKAPHGEATMGDFLLDGVPGEFAHLTRHLQGVQG
                     SAKTGQAHWNVAAASEKGEKRTLEFRGEEGRQGPYPLLTLATGESNEGIVAGSEAIWL
                     DGARLKRGADADYVIDYGASTVTFTIHRPITAQSRIAVDFEAASSRYRRSLYAASTQG
                     LVPGRGGWYANYLSEGDDPSSPIGAALTADDRAALSALGDSADSILPSGVRYAGPGLG
                     SYDWDESDPANPHWVFLGSSHGTYDVDFASVGAGEGAYSDTTSVEGVRFYRYRGVNLG
                     SYAPGRALAVPSSRRLVDVGGSARLFGALGVDGEVARSGFDKNALSSRNDGDNAGTAA
                     RFEARLDPRAVAIGGLKLGSIQAQGRMRSRGDRFESFDRTDPAFEYERWNQAPSSAGE
                     DRQELSLQYTPVQALALRGDVGRRRVTGGSESVRRGAQAVLNSALTGTLSWEEARNAT
                     SGSAGLRSLWGGDLGRDQGWFQPRVTAKDERIEGQDGDSVDARWNRDLGMILRLVPGK
                     ALKLRGGYARHEGSVTDAGTVAEDQAFTWDGGLSARFGSSFSLDGGFSRRRAESSTGP
                     QATNLAQLAVQAGRPGAPVSSELRYDVNQLREASLVRELKPVGDGSGSYDAYGNPRLG
                     GGYELVTSTGDPTTRSRAVVQVRLDAFPSRAARAPGKTPALWRGLGGSTFLRVEALST
                     LPLGSPDQAFRPDAYLDPETTIRGDVTARQTLEFAPAGRRYDLRGEVGYRREQSAEIE
                     DLTTERRTGDARMTLRHPIPGGFRGTWTATYDRARQSIERQDATGSYASQMRGRGFEG
                     EIARELRGFGQVSILARQRRDIDLTHGGYFDLWSAGPTARYAGGAKLRVDARALYGWS
                     AQQGVYAPPGLYTTAPVGGRLDYDLLGEYRIHDRVSLSLNWTGFQAPNRPAYYTGRFE
                     LKGSF"
     gene            complement(4104..5090)
                     /locus_tag="VFR25_06520"
     CDS             complement(4104..5090)
                     /locus_tag="VFR25_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9940737.1"
                     /translation="MSGRAPAVTFPLRSIVLAPLLAVALVPAAIRAAPDVGTESRPGP
                     PHALRAERVLDAATLDRLGVPHPTRIVFDANHCLYVLDAAARRIVKLNPEGQPLWDLG
                     GYGDDDQSFSLPSDLAIDRRQSLLVLDRGKNALVAFDPAGHFLGVRSFGETVATETAD
                     PGARLLVDPFGDLWLLGPRDRDLIPLDDRLERSRRSRFLAPEASAGSPEAAAFLPTSG
                     GWFFDAGGRKLRRFGNNGQIASAVADSLAPSGAGDVAVDQAGFLYAADPGGQRLLVLD
                     DQGALRIERALGGAGVPWHPVAVAVSRSDRVAILDSERGEIQILTIERERVP"
     gene            complement(5134..5907)
                     /locus_tag="VFR25_06525"
     CDS             complement(5134..5907)
                     /locus_tag="VFR25_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940738.1"
                     /translation="MLAFADTASPARAAWAPLPAIYGEAWLAQTTDPASPPPAMGSAP
                     APPPTTSKKALLYSLILPGLGEMSMGEKGRATGFFIAEGLIWANFTYWTVAGNLRQDD
                     YIEQAQLNAGVGVSKESDDYWRLVGQFTSSSGEGPGSYEEDLRREARDTYPNDPAAQD
                     AFVAEHLPTGDRAWSWSTPALQSEYTNTRETSNRAHNKAKYSFAAAILNRVVSVIDVQ
                     MLRHKASKAAQGESAIRAQTRVYADAVEDGGRLVVERRF"
     gene            complement(5960..6607)
                     /locus_tag="VFR25_06530"
     CDS             complement(5960..6607)
                     /locus_tag="VFR25_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940739.1"
                     /translation="MHPGTVVDDQNPVLDTEHHASSGRAFIYNLILPGTGHLYAGYKR
                     GWAHLGVEGATWLTYFYYHDRGAQKENQFQAFADDHWDYDKWIADCGCAGSPQDSIIT
                     YFKDNNTQQYYEDIGKLDYYWQGWDDYNNTKQDSDNRRYYYGIRNDSNNFFKNARYAV
                     VVAFVNRIVSAVDVLRLTKKEPTLNIGSDATLHFKVRTKPFNSENAFGVRLVKKY"
     gene            complement(6831..10256)
                     /locus_tag="VFR25_06535"
     CDS             complement(6831..10256)
                     /locus_tag="VFR25_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9940740.1"
                     /translation="MTARRGFATGALFIILGSQAWMGLAPSVSQAAPNGRASLKITRG
                     PQAARVAPGRAALPPSVLQRKIALRSAFPDHFGPAGQAAPWITGGRLPLLAPRAGKLA
                     RTTPFVSGIPETIRVVGIRIDFQNDSLGPQTSTPDGRFDMRDGKALGVVIDPPPHDRN
                     YFMSHMEAMARYWRDQSFGNLVITYDVYPHADSSAYRLRDTGAYGPWTLGQSSYFEAT
                     RFFKDAVTAADNADTIPWDQFDVVILFHAGSDFQADVLGDSQRDIPTFQIGLNDSIEV
                     SGGLFLRGGVVMPETENQDGYFGALNGTMAHEFGHTQGLPDLYDINTFFPAVGVWSNM
                     DSGYLLGSAVQVESTGEIVLASGILPVSLDPWCKTTLWPDGIQTVDPGHALTTSLRSS
                     QLSNRVLKVPLGSDEALLIENRQTDLNHDNTIYLDRDSTTDVILGPGLSSVDPADSLG
                     NREYDFLLPGDGILMWHIDETVICTPIDNTTFLCGPNANPDLGVNSNPERRGVRVMEA
                     DGIADIGDPNSAYFFGSPFDPYYVGNHTRLDDDSNPSARTNDGGRSHVRVNVLSAPDV
                     DMGIQVDSEWRLAGWPVSAQHELTGSAPTYGSFLHDGTRSVVTAADSLIFAWAADGSP
                     WYSSRPTGEWAAAPSRLLLPVLFADSLYRPNAGIAVHGSAVLATGVDGRVYAYRPRDR
                     TDPSSLLIPGWPPALDENDLSVLATTPPVLAPGGAVIVGASDGRVFAITPSEDPLQPP
                     RLTEIADTLVVGGSPAAFPVSSNLAVGRFVGAGGYDVAFALSNGTIRVVEQAGKGTGR
                     FIASWPSGGPNYAPYLLGVDMDRAPDRHLELIAADPAQGTIHCYDMTGAEREGWPVVV
                     IAGIGGPVAAGDMDGDGFPEVLAVDLQGNAHRWNRNGVELQGWPVPLGSRFGTYATGG
                     TGSPLLGDVDQDGRPEAIIALGNGIVVALEPDGRVTSGWPISVPGAADDTPLLTSLNG
                     ADLPPDPPGAAWTHLVAGGGFDGSLAAYQLPARADSAFFTTDGVSSRFPWQEWGGNRR
                     RTSVLEDTVLVTAAPVASALAPGSVYCFPNPARGTEIGVAYTLGAGVTEVVIRVLDPT
                     GREVMRLTPSTAPTQNVARILLNNLASGVYVVRLEAKRDGASDVQFQKFAVVR"
     gene            complement(10264..11235)
                     /locus_tag="VFR25_06540"
     CDS             complement(10264..11235)
                     /locus_tag="VFR25_06540"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET9940741.1"
                     /translation="MSRILLPACLALALIAGAFGSAQAQSEAGAQSLLIAPGARSDAM
                     GHTGVAFVGDGNAVWWNSAALAFVRGHDVALTYTKLVPDLADDVNFSYIGYAQRVEGL
                     GGLGASFGYLSYGKSTATDADGNDIREFTSNEIAPSIAYGTDLLPNMGFGVALKLVRV
                     DLAPEDVTLDGRAGRGTTFAADFGWLYKMSQRASLGAGLYNIGPDIAYIDEDQSDPLG
                     RNVRVGFAFSPIDANVHHVTISADATRFLLPGRTLAIDQWGAGAEYEFNHLLALRAGY
                     YSDPVGTVTDFTYGFGLAYHNFKFDFANIPQSEFLDRVNRFSAGYHF"
     gene            complement(11339..15508)
                     /locus_tag="VFR25_06545"
     CDS             complement(11339..15508)
                     /locus_tag="VFR25_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HET9940742.1"
                     /translation="MIRRTHAVAAVLALGTALPLLARGSLAASAPAPVVSSVQPTGYK
                     VRVTLGEPSVVRGEFLGTPLAEIDVPGAVPERAAPGAPPLPARTVFLRIPWGARVRAS
                     VVPSPARSLGTLRPAPFPYLLTDASMRERVTAADLEAALQAQGSWRAPSVQGLATTRV
                     MAARGERLLAVTIRPVSWDASSGSASFVDEVAIEVTWDGPVEPLAGPASARASTAPSG
                     AVGPYYPSRAPRTAPASAGPARGARSLAGRAAAIGPLRVTPDPLRTWVRLNVLRPGLY
                     RVTASDLAAAGVGVGAIDPATIRIFRSTPGDLPENVNVDQGPDSLRECAIEVTGAGDG
                     SLDPADRIYVYATGSTGFGYDLVFGGGPAYQETQHSDVETLWMTWGPGPVATPPRRMA
                     TRSAALDAVAAPTYLAVPHRVHYEENRLYNADLFEPGVRWERWFNRILFQGSRVRFDL
                     TMPGAQPGGSADVLVRVWGVGSSIGAGLPDHVARIYWNRALVDTAGWDLSLHQDLAAT
                     GVITQGRDTLDVEVPALIDPGPFPQRSDRSYLAWFEIGYPRQLLAVNDTLQFAAPDSL
                     PNAPVRYQITGISDTSAVWLLDRTDPESPVRLVGGFVSGGAAPFAWTVEDSAGDGYRP
                     RYSLVSTTRAATPGISVYAPTSSANTISDLLDPSNEADYVIVAPPAFLAQAEALVAYR
                     SLGIEGIPAPRVRIATTERVFAQLGAGLPSPVAIRNLMQYAAAHWTGVPPSYLCLLGD
                     ATFDPKNYIGFGTPDLVPTYSNYYDVSQTQQYNSDDFYGILDGPGDLLLDLAVGRLPA
                     GTTAEAQTLTAGKLRLYEESQDFDMWRARGILCADDAVQRDEIDPVGNEHVRQSERLD
                     GLHLPYPVERAKVYLNDFAFSDTTHQSKPAAREEFIAQINRGAWLTNYVGHGSDQVLA
                     DEQVFRSADATRLTNATRPSIFGYFSCTVGKFDEPSGEGLGELLLTLPGGGAVASLAA
                     SGLTLPSSSGPLNESFLDELFPFAPRVDTLRTAGLAFARAKNELAGPSNDTVRKYGFL
                     GDPALRPPLPRGAGVWEKQPLDSVLRGDVVTLRGHAVMGPADSTRDQVSMGNADLLLQ
                     GPPFIRSQFAQVSGTPQTYQIPGPTIFRGTVPLDHGSFEVTFVVPTDGRLVGRGGKLR
                     ALLSQAGGRGVGLAVDSLRIAAASSPRVDATPPTIQVVYPSGPDSLLHPGSHVTLVLS
                     DSSGIDLTRLDNAHSIFVIVDDRGTPYELTSGFAYDQGSYTTGQVDFTIPAGLQDGAH
                     RLEIHASDTFRNIGVANVIVDVATEAVSGTALRLDQVFNYPNPFPRETYLHARLNQPA
                     KLRIKVLTVAGRRVREIEWDGAAGENYIPWDGRDSEGENVAIGVYLFSVTAESSSGGR
                     AKAVGRALRTQ"
     gene            complement(15514..15675)
                     /locus_tag="VFR25_06550"
     CDS             complement(15514..15675)
                     /locus_tag="VFR25_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET9940743.1"
                     /translation="MSDQLLAILVCPQCRQDLEYDRAAGTFTCHHCRLRYSIVDGVPN
                     FLIEEAERI"
     gene            complement(15748..16737)
                     /locus_tag="VFR25_06555"
     CDS             complement(15748..16737)
                     /locus_tag="VFR25_06555"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET9940744.1"
                     /translation="MNGTWLDFEKPIIELERKIEELKSRAGGDERETAEELDRLEKKA
                     DRLRKDIFSKLTRWQRVKLARHPRRPYTLDYLKVMAPQFMELHGDRRFADDPAIVGGL
                     GMLDELPVLFLGHQKGRDTKENIHRNFGMANPEGYRKALRLMRMAANFGFPIVTFVDT
                     PGAYPGLGAEERGQSEALARNILEMAHLPVPIVTVVIGEGGSGGALALAVGDVVMMLE
                     NSIYSVITPEGCAAILWKDASKAEQAAENLRLTAQDLLELKVVDEVIPEPLGGAHRDP
                     EETARRVKETVVRHLKALIGLPKQELLDRRLEKYLKMGVYLEETAAAGATQEG"
     gene            complement(16734..20204)
                     /gene="dnaE"
                     /locus_tag="VFR25_06560"
     CDS             complement(16734..20204)
                     /gene="dnaE"
                     /locus_tag="VFR25_06560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9940745.1"
                     /translation="MSQPFVHLHNHSDYSLLDGACRLDRLIARAAAMKMPALALTDHG
                     NLFGAVEFYDAAHAAGVKPIIGCEVYVAHGSRADKMRTADGRGAYDHLVLLARNREGY
                     RNLTKLSSAGYLEGFHYKPRIDKELLAEHAGGLLCLSACLRGEVPQLVVADDMEGAQK
                     AAGWYRDLFGPEFYFFEIQDHGLEEEKKAARGLIELSRSMGIPVVATNDCHYLAREDA
                     EAHEVLLCIQTGKTMLDPDRFRFTTDQLYVKTPDEMEALFGETPEALTNTLVVAERCE
                     LTLDTERVQLPSFPIPPEFESEEVYLRRLAREGLDQRYPGGITEEIEKRLEYELDTIC
                     QVGYARYFLIVRDFIHYARENGVGVGPGRGSAAGSLVSYTLAITDIDPIKFDLLFERF
                     LNPERVSMPDIDIDFAYEDRGKVIDYVVGKYGRDSVSQIITFGTMAARAVVRDVARAL
                     GMTFAEGDKVAKMIPPDLGMTLQRALETVPELKALLQDERHARLLRCSMALEGLARHA
                     STHAAGILIAPGNLTEHVPMYRSPKGDFTTQFDMKSLERVGLLKMDFLGLRTLTVLEQ
                     AVELAREATGDAIDLGSLPLDDPDTYALLQKAQTVGIFQLESGGMRDLLRKVLPDTFE
                     DIIAINALFRPGPIQSGMLDDFAKRKHGKQKVTYMHPQLETLLKKTYGVIVYQDQVMQ
                     IANRLAGFSLAQADLLRRAMGKKKPEEMARQKTAFMEGCAKNNVPGKKAEEIFDLMDK
                     FAGYGFVRSHSAAYAMLSYQSAWLKAHHAAAYLAASLTSEAGDTDRIVTLVEEARRLG
                     IAVLPPDVNASVASFTLEGPAIRYGLAAVKNVGQGSVEAIVRARHEGGHFRTLADFIR
                     RVDLAAINRRVLDSLVQAGACDSLEGDRAQLLAAVGDLLAQAQERARAVNANQESLFG
                     AEHEIVAPDPPLPKVDPWPLDERLRREREVLGFYFSDHPLTPYRPYIEARATTEISRL
                     REARDLAEVSLVCLVQARKAHTDRNGRPMAFVTLEDLKGTVETTVFADLFEKHRADLE
                     PGAVLEVRGRVNLREDAEPKMVLTAVKPLGPPPSGAAVHIDLVRGGEKVSLEEIRALL
                     VRHPGISPVYFTVRGETDPGPTQIRAKRLLVSPNEELLGALRERLGAEAVRVVERAEE
                     VAVTPMSGAA"
     gene            20340..20909
                     /gene="pyrE"
                     /locus_tag="VFR25_06565"
     CDS             20340..20909
                     /gene="pyrE"
                     /locus_tag="VFR25_06565"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9940746.1"
                     /translation="MTSVSSAGPDLVQARARLRAILLERSIRFGDFTLSSGAKSRYYA
                     DVRRTSLHPEGALLIAQLLDPILQEHGVASIGGLTLGADPIVGATIAWTQLQGRGLVG
                     FLVRKEQKAHGMGNRIEGPFDPSLPVAIVDDVITKGGSALTAYLAAKEAGADVRVVAC
                     VVDRNEGGGEEFAKLGVPFRPLFRIDEFL"
     gene            complement(20946..23516)
                     /locus_tag="VFR25_06570"
     CDS             complement(20946..23516)
                     /locus_tag="VFR25_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET9940747.1"
                     /translation="MDRTEPSEPRRPPKPRPARQTTPAKPPPGKPGSGKRWWEWRPSW
                     PPSWSSWRPSWAPPGTLRWLLFLAAVAFCFLGYDWFRLSSIGSSRYVGQGWKFPTRVY
                     ADWKDYRVGDALQSSTLQRLLDRAHYQRVTKRPADPGRYRVNGPIFEVYLRAFTYPDR
                     VEHGTPVTVRLQNGRVESLGNGFGGNAEGRGLLRIEPELLGEFADPARERRSYVPLDR
                     LPRHLILATIASEDRRFYHHWGLDLLGMGRATMRNVRARTVVEGGSTITQQLVKNFFL
                     THERSIWRKFHEALLAVLVELRYSKDQILEFYLNQIYLGQRGSWSVCGVEEASLYYFN
                     KHAQELTVPEAALIVGIIPAPNRFSPYRDAEAAIVRRNEVLQDMAEAGYISKQEARKY
                     RRTQMRFAANPPPATLAPYFVDYVQETLSKDIPESELSGRGFTIFTTLDSGLQEEGEK
                     ILRNGAKEADWRSPSGGAERDPAQGALIAIEPSTGNIRAMIGGRSYAESPFNRAVDSR
                     RQPGSAFKPFVYIAALDSYFAGRRPPLTAATVLEDAPDTFQTDLGPWAPRNFENSYAG
                     RVTAARAIARSLNVATVHLAQMVGMNRVVDVARSLGIQSRLRAVASLALGTSELSVLE
                     LTTAYATIANGGVLVEPTPVKAVLDRSGSVRWSPKRESRRVLHPETAYMATILLEGPV
                     IYGTAAGVRTEYGFVRPAAGKTGTTDDENDAWFVGFTPDLATGVWVGCDKNRRLGLTG
                     TQAAVPIWARFMDVAHRDRPLRDFEPPPDVVDVWIDGDTGYRAGPGCPHVMQAAFIRK
                     TEPRQVCPVFHPVVWPDSLGGFMSDSTGTPDTQKENQQPEQPPDEETPPPDDQGGQGL
                     "
     gene            complement(23593..24936)
                     /locus_tag="VFR25_06575"
     CDS             complement(23593..24936)
                     /locus_tag="VFR25_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940748.1"
                     /translation="MAGFADPVSPPLPPTLAPPAVEPGLVLEPITPTLSTAPDPEASF
                     ADLALESKALPTAPPKPETSSMGERPSLIPPMPSKKAWDFEPVDPPRTSSPPEESLNF
                     AEEALNRSSQREGSRAVFRIVLWSCGALAGVALLAVSVWMAMAHRETANDPQALAPPP
                     HGAADSASVGSTAMKDSIAIGAAQTPVTPTIGAPSGTAPSQPGAAQSGTVQPGATHTG
                     SAAPNATAASKPTKPQPSTTAAGAATKPGAKSIASSSKPPHDAPTTETTAPPGTVPPR
                     RGPPTREEGFEPVVDGPADTKGTWPQTDFTPAPTTKKPPATTKKPSFIVEQPPEEVRP
                     ATPPDEADTSGPVGRLHLATVKAHEDRDIEALRKIKASWKSYLRTALGTERERSKREY
                     ADCLWAIQEITGRVADRKEALAAYREYVLHAPAGGVDARSVARMRHLEDVLSDSQ"
     gene            complement(25279..25476)
                     /locus_tag="VFR25_06580"
     CDS             complement(25279..25476)
                     /locus_tag="VFR25_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9940749.1"
                     /translation="MAYEIVKDICEGESSCVDVCPVDCIKKGDGENAKATGWYLIVAE
                     DCVSCNACLEACPVEGAILPD"
     gene            complement(25520..26611)
                     /gene="nadA"
                     /locus_tag="VFR25_06585"
     CDS             complement(25520..26611)
                     /gene="nadA"
                     /locus_tag="VFR25_06585"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET9940750.1"
                     /translation="MSPRVLTVPEPYLELDDAALASRIREAKARLGSSLVVLGHHYQR
                     DDVIQHADITGDSYKLARLGAERTDAKWIVFCGVHFMAESADILRQPDQTVVLPDLNA
                     GCSMADMAPTDDVIEAGAALERLGLSSVMPVTYMNSTAEIKAYCGERGGAVCTSSNCR
                     AIFEWSFAQRDQIFFLPDEHLGRNTAIAMGIAPERIVVWDPKRPLGGSTEDELRRAKV
                     IVWKGCCSVHTKFHLQHVLDRRAEDPEVKILVHPEVPHEVVAASDAVGSTETIIRTLR
                     EAPAGSHWAVGTEYHLVQRLAHSMPDKKIEILSKEFCLCATMYRISPQNLAWALDSIV
                     EGRPVNVIRVPETTKRWAHVALDRMLQVH"
     gene            26815..28617
                     /locus_tag="VFR25_06590"
     CDS             26815..28617
                     /locus_tag="VFR25_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET9940751.1"
                     /translation="MSAIDNRFTIHVATVNGSGSQTANTTLLRALFQMGIPVSGKNLF
                     PSNIAGLPTWFTIRANDQGYVARIKRADLLVALNPETSVADVAAMEPGSVLVVADGLP
                     MPAVPPDRTLYKVPFTKLVEPLVADAKLRKLVVNMIYVGVVSWLLGIDPDEVDRALVA
                     QLGRKPKALEMNRAAVRVGIEYAEKHMMRTPFQARRADRTAGKILIDGNQAAALGALF
                     GGVTFLSWYPITPSSSLAEQLEGFLARYRKDPKSGKATYAVIQAEDEISAIGMVLGAS
                     WAGARAMTTTSGPGLSLMTEFTGYAYYAEIPAVIWDIQRIGPSTGLPTRTSQADILSV
                     AFLSHGDTKHVILFPATVQDCYEYGMRAFDLAERLQTPVFVLSDLDIGMNTWMSQPFR
                     YPENPWDRGKVLSASDLERVKKFERYRDVDGDAIPYRTLPGTNHPLAAYFTRGSGHDE
                     AARYTESSEAYPRVMDRLARKFETAKTLVPAPFVEKTQGADVGLIAFGSTHHAMDETR
                     DKLRTEGTPTSYLLLRAYPFPEEVERFVRGHKRVYVVEQNRDAQMAALLRMEYPELAP
                     RIRSVLHYDGLPIDAESILNGVRSAERPSEVTTR"
     gene            28614..29657
                     /locus_tag="VFR25_06595"
     CDS             28614..29657
                     /locus_tag="VFR25_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET9940752.1"
                     /translation="MSTPPNVNRIGLTAADYKGSKSTLCPGCGHDAITGSIIQAAYEL
                     GLEQHKVAKMSGIGCSSKTPAYFLGRSHSFNAVHGRMPSVATGAHLANRELIMMGVSG
                     DGDSASIGLGQFCHLVRRNVPIVYIIENNGVYGLTKGQFSATADVGTRAKSGQVNEMM
                     PIDLCEMAIQLGCGFVARSFSGDPKQLRPLLKAAFSHRGTSVLDVISPCVTFNNHEGS
                     TKSYTSAKEHDAPLHEVTFIPHFENIEIDYAPGESREVTLHDGSRIVLRKLEEDYDPT
                     DRMRAIDTIERGRVEDRLATGLLYQSKGNAPIGEDLGLVDEPLASLPLERVRPPREVL
                     DKIMAGLMTGGGA"
     gene            complement(29708..30457)
                     /locus_tag="VFR25_06600"
     CDS             complement(29708..30457)
                     /locus_tag="VFR25_06600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9940753.1"
                     /translation="MSWREPRPIVFGGPLAPGVRTLVFVTVGVYVFQLLAGFLAQVRL
                     ERLFGLVPYEVVHHLFLWQVGTYLFLHGGFFHILFNMLGLWMFGSELEWLWGTQRFVR
                     FYFITGIGAALCSILVTPNSTIPIIGASGAIYGILAAYGILFPDRTLLLYFVIPIKAK
                     YFVLILGALAFWATLTATGGGVANVAHLGGLLFGWLYMRRFGRGSGRGVSFRWRNPIR
                     AWRERVRQNRLRKKFKVYYRNTRGEDDGGDE"
     gene            complement(30454..31752)
                     /locus_tag="VFR25_06605"
     CDS             complement(30454..31752)
                     /locus_tag="VFR25_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HET9940754.1"
                     /translation="MNLCLFEDPLVQSFGPHTLLRPAFALRCGAFSLVEKLVRAFPES
                     PLVLHVRPEIEEVTRLLYPTAHVGAPPAEETLFVNARLCMTDDEILHFLAASPSEASY
                     MADGTLFAAKVKAERVVHAARYLREGDPERAFEDLRFPAEVNAFLARSAADLIRWSPR
                     QIVQDFRHGFQPGTVRGTIEPGVHVTQPEAVHVARGARVQAGAALNAEGGPILIRENA
                     KVEPLAYIEGPAVIGEGSVVRAGARIRGGTSIGPVCKVGGEIEASVFQGYANKQHDGF
                     LGHSFVGEWVNLGANTNNSDLKNNYGEVRFYRSARAYADGHAEGSGQTLLGAVIGDYT
                     KTGISTSLPTGCVLGIGSNLYGAELWPVYVPSFVWGTPSSLAEHRIDAMIETAVRAME
                     RRKVDLSVELDSRIRQAFDDTAEERRLYLGPKLPAPEKRA"
BASE COUNT         5007 a        10751 c        11092 g         4976 t
ORIGIN      
        1 ggatgacgac gtggccgtgt ccgggctcgg accccgccac gacgcggggc tcgcccacga
       61 acgcgaagag atcgctggcg cgaagccggt cgcgggcttc cgtgaccagc tcgggcgtga
      121 gcacgcgccc ctctcgaagt cccgcgatcg tcgccgcgga ccgcggcttg gtcacggagg
      181 cgccggggat ctcgaggctg tcgaagacga ccgggggccc ggtggagagg cgcacgtgga
      241 cgcggacgga gtcgccggcg gggacgatcg agtcgagact caccgtggcc gcgtagcgtc
      301 cgaggtcgac cgccgcgtcg cggagcgcgt cgagcccgcc cgcgatcgcc tgcggagaca
      361 cgccaccttt ggccccgcgc gcgaacacgc ccgcagggtc gtggatcccg acggcgccgg
      421 gaggagcaag cctcaatgcg ggagccagcg gaggcgctgg cggagccggc gcggtccctt
      481 cgacccgtat cgtcgcggtc ccgggggtgg tgccgcttcc ctcctcgagc gtgagtcgca
      541 cgacggcgtc atagcgcccg agcagtgcga gccggtcgcg caggcgtccg gcgaggcgct
      601 cgatctcggt gcgctgccac agccgctcga gcggcgccgt ctcgccggtc tcgccggcga
      661 tcacggaatc ggccacccct tcgacatgga gcgcgaagcg cgggtcggcg gatgggccgg
      721 cttgatccga ggcgcacgcc cttcctgccg gcgtgaacag aatcgccgcc gcgagcgcga
      781 ggatgagcat cacgatccgc ttcacgtcac gatcgcgcgc gcgcatcaga agctcccctt
      841 cagctcgaag cgtcccgtgt agtacgcggg gcggttcggc gcctggaatc cggtccagtt
      901 gagcgagagc gagacgcgat cgtggatccg gtactcaccg aggaggtcgt aatcgagccg
      961 cccgccgacc ggagccgtcg tgtagaggcc aggaggcgca tacactccct gctgcgcgct
     1021 ccacccgtag agagcgcgcg cgtcgacccg gagcttcgct ccgcccgcat agcgcgcggt
     1081 cgggcccgcg gaccagaggt cgaagtagcc gccgtgcgtg agatcgatgt cgcggcgctg
     1141 ccgcgcgagg atcgagacct gcccgaagcc gcgcagctcg cgtgcgatct ccccctcgaa
     1201 gccgcggccg cgcatctggg aggcatacga gcccgtggcg tcctgccgct cgatcgactg
     1261 gcgcgcgcga tcgtaggtgg ccgtccaggt gccgcggaat ccgcccggga tcggatgccg
     1321 cagcgtcatg cgcgcgtctc cggttcggcg ctcggtcgtg aggtcctcga tctcagcgct
     1381 ctgctcgcgg cggtagccca cctcgccgcg caggtcatag cggcgccccg cgggggcgaa
     1441 ctccagcgtc tgccgtgccg tgacgtcccc gcgaatcgtc gtctctggat cgaggtacgc
     1501 atcgggacgg aacgcctggt cggggctgcc gagcggaagc gtcgagagcg cttccacgcg
     1561 aaggaacgtg ctcccgccga gtccgcgcca gagcgcgggg gtcttcccgg gcgcgcgggc
     1621 ggcgcgcgac gggaaggcgt cgagcctcac ctgcacgacc gcgcgcgagc gcgtggtcgg
     1681 atcgccggtc gacgtgacga gctcgtagcc gcccccgagt cgcggattgc cgtacgcatc
     1741 gtacgatccg cttccatctc cgaccggctt gagctcgcga acgagcgagg cctcgcgaag
     1801 ctggttcacg tcgtagcgga gctccgagct gaccggcgcg ccgggccgtc ccgcctgcac
     1861 ggcgagctgc gccaggttcg tcgcctgcgg ccccgtcgag gactccgcgc gccggcgcga
     1921 gaatccgccg tcgagcgaga aggacgagcc gaagcgcgcg gagagtccac cgtcccaggt
     1981 gaacgcctgg tcctccgcca ccgtcccggc gtccgtgacc gagccttcgt gccgcgcgta
     2041 gccgccgcgc agcttgagcg ccttcccggg gacgaggcgc aggatcattc cgagatcccg
     2101 gttccagcgc gcgtccacgc tgtccccgtc ctgtccttcg atccgctcgt ccttcgcggt
     2161 gacgcgcggc tggaaccatc cctggtcgcg gcccaagtct ccgccccaga gcgaccgaag
     2221 gcccgcgctc ccgctcgtcg cgttgcgcgc ctcctcccag ctcagggttc cggtgagcgc
     2281 tgaattcaag acggcttgag caccccgccg caccgattcc gatccgcccg tcacgcggcg
     2341 gcggccgacg tcgccgcgga gcgcgagggc ctgcaccggc gtgtactgga gcgacagctc
     2401 ctggcggtcc tcgccggcac tcgacggcgc ctggttccag cgctcgtact cgaacgcggg
     2461 atcggtgcga tcgaacgact cgaagcgatc gccgcgcgag cgcatacgcc cttgagcctg
     2521 gatcgacccg agcttgagtc cgccgatcgc gaccgctctg ggatcgaggc gcgcctcgaa
     2581 tcgcgcggcc gtgccggcgt tgtcgccgtc gttgcgcgac gagagcgcgt tcttgtcgaa
     2641 gcccgaccgc gccacctcgc cgtccacgcc cagcgcgccg aagaggcgcg cggatccgcc
     2701 gacgtcgacg agacggcggg agctgggcac ggcgagcgct cggcccggcg cgtaggagcc
     2761 caggttcacg ccgcggtagc gatagaagcg cacgccctcg accgacgtcg tgtccgagta
     2821 ggctccctcg cccgcgccca cgctcgcgaa atcgacgtcg tacgttccgt gcgatgagcc
     2881 gaggaagacc cagtgcggat tcgccgggtc ggactcgtcc cagtcgtacg acccgaggcc
     2941 gggaccggcg tagcgcacgc cgctcggaag gatcgagtcg gccgaatcgc cgagcgcgct
     3001 cagcgcggca cgatcgtcgg cggtcagcgc cgcgccgatc gggctcgacg gatcgtcccc
     3061 ctcgctcaag tagttggcgt accagcctcc gcggccgggg accaagccct gcgtgctggc
     3121 cgcgtagaga ctccggcggt agcggctcga ggccgcttcg aagtcgacgg cgatccggct
     3181 ctgcgccgtg atgggacggt ggatggtgaa cgtcacggtg gacgcgccat agtcgatcac
     3241 gtagtcggcg tccgcgccgc gcttcaggcg cgcgccgtcg agccagatcg cctccgagcc
     3301 ggccacgatg ccctcgttgg actcgccggt cgcgagtgtg agcagcggat acggaccttg
     3361 ccggccctcc tcccctcgga actcgagggt ccgcttctcc cccttctcgc tcgccgcggc
     3421 gacgttccag tgtgcctggc ccgtcttggc cgatccctgc acaccctgga gatggcgcgt
     3481 gagatgggcg aattcgcccg gcacgccgtc caggaggaag tcccccatcg tggcttcgcc
     3541 gtggggcgcc ttcagggaga gcgtgatgtt gtcgagatcc tcgagctcgc ggctcgatcc
     3601 gtccggctcg aagggcaggc ggcggtcgct caagctcgcc gcgacctgca cgtcgcccgc
     3661 gatccgcccg cggatcgtga gatcgagcga ctgcgtcatc gacgcggcgc gattcgcgcc
     3721 aacctcgacg ctcaccagct tgagtccgct gaacgtgatc gcgtcgttcg aagccgcgtc
     3781 tgcgatcgtt ccccctgctg cgcccgcggc gggcgcggcc gcgatccagg agcgggtctc
     3841 gttgcgaagg acgctggccg tcgaatcggg cggcgcctga tccgccgcgc cggcggcaag
     3901 cggcccgctc gcccggagcg cgaggagcga cggcgctcgc gccacgggca gctcgatcac
     3961 gacggtatct cggggtgcgt cgcaaccccc gggacagagc gagtcgatgg ggcgcgcatt
     4021 cgcgggaagc ggaccgagcc cccaggcgag cgcagcgccg atcgaggcgc ccatgacgat
     4081 gcgggaaccg aagcgcgatt cgatcacggc acgcgctccc gctcgatggt caggatctgg
     4141 atctcaccgc gctcggagtc gaggatggcg acccggtccg agcggctcac ggccactgcg
     4201 accggatgcc acggcacgcc cgcgccgccg agcgcgcgct cgatccggag cgcgccctgg
     4261 tcgtcgagca cgagaagacg ctgcccgccc ggatccgctg cgtagaggaa tcctgcctgg
     4321 tccacggcga cgtccccggc gccgctcggc gccagcgagt ccgcgaccgc cgacgcgatc
     4381 tgaccgttgt tgccgaaccg acggagcttt cgtccgcccg cgtcgaagaa ccagcctccc
     4441 gaagttggga ggaaggccgc ggcctcgggg ctgccggccg acgcctcggg cgccaggaag
     4501 cgcgatcggc gcgagcgctc gagccggtcg tcgagcggga tgagatcccg gtcgcgcggg
     4561 ccgagcagcc acaggtcgcc gaacggatcg acgaggaggc gcgctccggg gtcggcggtt
     4621 tcggtcgcga cggtctcgcc gaaggagcgg accccgagga aatgccccgc cggatcgaac
     4681 gcgacgagcg cgttcttgcc ccggtcgagc acgagaaggg attgcctgcg atcgatcgcg
     4741 agatccgagg gaagcgaaaa cgactggtcg tcgtcgccat agccgccgag atcccagagg
     4801 ggctgcccct caggattgag cttcacgatc cgccgcgcgg cggcgtcgag cacgtagagg
     4861 cagtggttcg cgtcgaagac gatgcgtgtg gggtgcggca cgccgagccg gtcgagggtc
     4921 gcggcgtcca gcacgcgctc cgcgcgcagc gcatgcgggg ggccgggacg gctctccgtg
     4981 ccgacgtcgg gggccgcgcg gatcgcggcg gggaccagag ccaccgcgag gagcggcgct
     5041 aggacgatgg accgaagcgg gaaggtgact gcgggggcac gaccgctcat cgaattccgt
     5101 ggcgcgtgga gcgcggcgga gcggtggata tcgctagaac cggcgctcga ccaccaacct
     5161 cccgccgtct tcgacggcat cggcgtacac tcgcgtctga gcccggatcg cgctctcacc
     5221 ctgggccgcc ttcgaagcct tgtgtctcag catctggacg tcgatcacgc tgacgacgcg
     5281 attcaggatc gcggccgcaa aggaatactt cgccttgttg tgcgcgcggt tgctggtctc
     5341 gcgggtgtta gtgtactcgg actggagcgc cggcgtgctc cacgaccagg ccctgtcccc
     5401 cgtcggcaga tgctccgcca cgaaggcatc ctgggccgcc ggatcgttgg gatacgtgtc
     5461 ccgcgcctcg cgcctcagat cctcctcgta gctcccgggc ccttccccgc tcgaggacgt
     5521 gaactgcccc acgagacgcc agtagtcgtc gctctccttg ctgacgccca cgcccgcgtt
     5581 gagctgcgcc tgctcgatgt agtcgtcctg acggagattc ccggcgacgg tccagtaggt
     5641 gaagttggcc cagatgagcc cttcggcgat gaagaagccg gtcgcacgtc ccttctcccc
     5701 catcgacatc tcgcccaagc cgggaaggat gagcgagtag aggagcgcct tcttgctcgt
     5761 ggtcggcggc ggggccgggg ccgagcccat ggcgggcgga ggcgacgcgg gatcggtcgt
     5821 ctgggccagc caggcttcgc catagatcgc gggcaagggc gcccacgccg cgcgggccgg
     5881 cgatgccgtg tccgcgaagg caagcacggg cgaggccgcc gctaggagcg cggcagccag
     5941 caggaaccgg aaggtgatct cagtactttt tgacgaggcg tacaccaaaa gcgttctcgc
     6001 tgttgaacgg cttggtcctc accttgaagt gaagcgttgc gtcggagccg atgttgaggg
     6061 ttggctcctt tttcgtcaac cgcaggacgt cgaccgcgct cacgatccgg ttcacgaacg
     6121 cgaccaccac cgcgtagcgc gcattcttga agaaattgtt gctgtcgttc cggatgccgt
     6181 aatagtagcg gcgattgtcg gagtcctgct tcgtattgtt gtaatcgtcc cagccctgcc
     6241 agtagtagtc gagcttcccg atgtcctcgt agtactgctg ggtgttgttg tccttgaagt
     6301 acgtgatgat gctgtcctgc ggcgacccgg cgcagccgca gtccgcgatc cacttgtcgt
     6361 agtcccagtg gtcgtcggcg aacgcctgga actggttttc cttctgcgcg ccgcggtcgt
     6421 gatagtagaa atacgtgagc cacgtcgctc cttccacacc caggtgtgcc catccgcgct
     6481 tgtatcccgc gtagagatgc ccggtgccgg gaaggatcag gttgtaaatg aaggctcgtc
     6541 ccgacgaggc gtggtgctcc gtatcgagga cgggattctg gtcgtccacg acggtcccgg
     6601 ggtgcatgtc ctggtggctc gtggaatcag caatggagcg ctgggcgagc agaatctcct
     6661 gaggcgtcgc gtcctcaaaa tccaatgggg aggcgtggac gcaaggagag acggctaggg
     6721 cggccccgag aatcgcgaag gcgatggcaa cgatgcgggt caggttacgg gtcccttcgg
     6781 ccggccggga tattgataac gacaacacct tattatatag acacataggc ctaccgcaca
     6841 acggcgaatt tttggaattg gacgtccgag gccccgtccc tcttcgcctc gagacgtact
     6901 acgtaaacac cgctcgccag attgttgagc aagattctgg ccacattttg cgtcggcgcg
     6961 gtggaggggg tcagccgcat cacctcccgc cctgtgggat cgagcacgcg aatgacgact
     7021 tcggtgactc ccgcgcccag cgtgtaggcc actccgatct cggtgccacg ggccgggttc
     7081 gggaagcagt acaccgagcc tggggcgagg gccgaggcga cgggggccgc ggtcacgagg
     7141 accgtgtcct cgaggaccga ggtccggcgg cggttgcctc cccattcctg ccacgggaag
     7201 cgcgacgaca ccccgtcggt ggtgaagaag gcgctatccg cccgcgccgg cagctggtac
     7261 gccgcgaggc ttccgtcgaa gccgccaccc gccacaaggt gcgtccacgc cgcccccggc
     7321 ggatcgggcg gaaggtcggc cccgttcagc gacgtgagga ggggcgtgtc gtcggccgcg
     7381 cccggaaccg agatcggcca gcccgaggtc acgcgtccgt ccggctcgag cgccacgacg
     7441 attccattgc cgagggcgat gatcgcctcg ggacggccgt cctgatcgac atcgcccagg
     7501 agcggcgagc ccgttccccc ggtcgcatag gtgccgaagc gcgaaccgag cgggaccggc
     7561 cagccctgga gctcgactcc gttccggttc caccggtgcg cgtttccctg gagatcgacg
     7621 gcgaggacct cggggaatcc gtcgccgtcc atgtctcccg ccgcgaccgg gccaccgatg
     7681 cccgcgatga ccacgaccgg ccagccctca cgctcggcgc cggtcatgtc atagcaatgg
     7741 atcgttccct gcgcgggatc ggcggcgatc agctcgagat ggcgatcggg cgcgcgatcc
     7801 atgtcgacgc cgaggaggta gggcgcgtag ttgggcccgc ccgagggcca gctcgcgatg
     7861 aatcgcccgg tccccttccc tgcctgctcg acgacgcgga tcgttccatt ggacagcgcg
     7921 aacgcgacgt cgtagccgcc ggcgcccacg aaccgtccca cggcgagatt gctcgagaca
     7981 gggaacgcgg cgggcgaccc gccgaccacc agcgtgtccg cgatctcggt gagccgcggc
     8041 ggctggagtg gatcctcgga gggcgtgatc gcgaagacgc ggccgtcgct ggcgcccacg
     8101 atcaccgcgc cgcccggggc gagcaccggc ggcgtcgtcg cgagcaccga caggtcgttc
     8161 tcgtcgagcg ccggcggcca tccgggaatc aggagtgagg acgggtcggt ccgatcccgc
     8221 ggtctgtagg catacacgcg cccgtccacg ccggtcgcga gcacggccga gccgtgcacg
     8281 gcgatccccg cattcgggcg gtagagggaa tccgcgaaga gcaccggcag gaggagacgt
     8341 gacggcgccg cggcccactc gccggtgggc cggctcgagt accacggcga tccgtccgcg
     8401 gcccacgcga agatcaggct gtcggccgcg gtcacgacgg accgggtgcc gtcgtggagg
     8461 aagctgccgt acgtgggcgc gctcccggtc agctcgtgct gggccgagac gggccaaccc
     8521 gcgagcctcc actccgaatc gacctggatc cccatgtcca cgtccggcgc ggagagcacg
     8581 ttgacccgca cgtggctccg accgccgtcg ttggtgcgcg cggagggatt gctgtcgtcg
     8641 tcgagccgcg tgtggttccc cacgtaatac ggatcgaacg ggctcccgaa gaagtaggcg
     8701 gagttcggat cgccgatgtc ggcgatgccg tccgcctcca tcacgcgcac gccgcggcgc
     8761 tcgggattgg aattgacgcc cagatcgggg ttcgcgttcg gcccgcagag gaacgtggtg
     8821 ttgtcgatcg gcgtgcagat gacggtctcg tcgatgtgcc acatgaggat cccatcgccg
     8881 ggaaggagga agtcatactc ccggttcccc agggaatccg cgggatccac gctcgacagt
     8941 cccggcccga gaatcacgtc cgtcgtcgag tcgcgatcga ggtagatcgt gttgtcgtgg
     9001 ttgaggtcgg tctggcgatt ctcgatcagg agcgcctcgt cggagcccag cggcaccttg
     9061 agcacgcgat tcgagagctg cgacgagcgc agcgaggtcg tgagcgcgtg cccgggatcg
     9121 acggtctgga tcccgtcggg ccagagggtc gtcttgcacc agggatcgag gctcacgggc
     9181 aggatgccgg acgcgagcac gatctccccc gtcgactcca cctgcaccgc gcttccgagc
     9241 aggtacccgg agtccatgtt gctccacacg ccgaccgcgg gaaagaacgt gttgatgtcg
     9301 tagagatcgg ggagtccctg cgtgtggccg aactcatgag ccatcgtccc gttgagcgcg
     9361 ccgaagtagc cgtcctggtt ttccgtctcc ggcatcacga cgccgccgcg caggaagagt
     9421 ccgccgctca cctcgatcga atcgttgagc ccgatctgga acgtggggat gtcgcgctgg
     9481 ctatcgccga gcacgtccgc ctggaagtcg gagcccgcgt ggaagaggat gacgacgtcg
     9541 aactggtccc acgggatcgt gtcggcgttg tccgcggccg tgaccgcgtc cttgaagaag
     9601 cgcgtcgcct cgaaatagga cgactgcccc agcgtccagg gtccgtacgc ccccgtgtcg
     9661 cgaagccggt aggccgagct gtcggcgtgc gggtagacgt cgtaggtgat cacgaggttg
     9721 ccgaacgact gatcccgcca gtagcgcgcc atcgcctcca tgtgggacat gaagtagttc
     9781 cggtcgtgcg gcggcggatc gatcaccact ccgagggcct tgccgtcgcg catgtcgaac
     9841 cggccgtccg gggtcgaggt ctgggggccg aggctgtcgt tctggaagtc gatccggatg
     9901 ccgaccacac ggatggtctc cgggatcccc gagacgaagg gggtcgtgcg cgcgagcttc
     9961 cccgcccgcg gcgcgaggag cgggaggcgt cccccggtga tccagggcgc cgcctgcccc
    10021 gcgggtccga agtggtccgg gaacgcggag cggagggcga tctttcgctg aaggaccgag
    10081 ggaggaagcg cggcgcggcc cggcgccacg cgtgcggcct gtggcccgcg cgtgatcttg
    10141 agcgacgcgc gcccgttcgg ggccgcctgg gaaacagagg gagccaggcc catccaggcc
    10201 tggctcccga gaatgatgaa gagggctccg gtcgcgaacc ctcggcgggc ggtcacttga
    10261 gctctagaag tgatacccgg cggagaagcg gttcacgcgg tcgaggaact ccgactgcgg
    10321 gatgttcgcg aaatcgaatt tgaagttgtg gtacgcgagt ccgaacccgt aggtgaagtc
    10381 cgtcacggtt ccgaccgggt cgctgtagta ccccgcccga agcgcgagga gatgattgaa
    10441 ctcgtactcg gcgcccgccc cccactgatc gatcgcgagc gtgcggcccg ggaggaggaa
    10501 gcgagtggcg tcggccgaga tcgtcacgtg gtggacgttg gcgtcgatcg gcgagaaggc
    10561 gaacccgacg cggacgttcc gtccgagagg atccgactga tcctcgtcga tgtaggcgat
    10621 gtcgggaccg atgttgtaga ggcccgcgcc cagagacgcc ctctggctca tcttgtagag
    10681 ccagccgaag tcggcggcga acgtcgtgcc gcgtcccgcg cggccgtcca gggtcacgtc
    10741 ctcgggagcg aggtcgacgc ggacgagctt cagcgcgacg ccgaagccca tgttcggaag
    10801 aaggtcggtc ccgtaggcga tcgacggggc gatctcgttg ctcgtgaatt cgcggatgtc
    10861 gtttccatcc gcgtccgtcg cggtgctctt gccgtagctc aggtacccga agctggcgcc
    10921 gaggccgcct aagccctcga cccgctgcgc gtagccgatg taggagaagt tcacgtcatc
    10981 ggcgaggtcg ggaacgagct tggtataggt gagggccacg tcgtgcccgc gcacgaaggc
    11041 cagggccgcg gagttccacc acaccgcatt gccgtcgcca acgaacgcca cgccggtatg
    11101 gcccatggcg tcgcttcggg cgccgggggc gatcaggagc gactgggccc ccgcttcgga
    11161 ctgggcctgg gcggacccga acgcacccgc aatgagggcg agagcgagac aggccggcag
    11221 taggatacga ctcattcgaa aacctccttc ggatgaaaaa aatagggacg cacacgcgcc
    11281 ccgacggaca tcaaggatat cgaagaacct ctggagggtc aagcggttgg ctccttgatc
    11341 attgggtccg gagggcccgt ccgacggcct tggcgcggcc gcccgaggag gactcggcgg
    11401 tgacggaaaa gaggtacact ccaatggcca cattttctcc ctcggaatcg cgcccgtccc
    11461 aagggatgta gttctccccg gcagccccgt cccactcgat ctcccggacc cgccggccgg
    11521 ctaccgtcag gaccttgatt ctaagcttcg ccggctggtt gagtcgcgcg tgcaaatagg
    11581 tctcccgggg gaacggattc gggtagttga agacctggtc gagccgtagt gccgtccccg
    11641 acaccgcctc ggtcgccacg tccacgatca cgttcgccac accgatgttc cggaacgtgt
    11701 ccgacgcatg gatctcgagc cggtgagccc cgtcctggag ccccgccgga atcgtgaaat
    11761 ccacctgccc ggtcgtgtag ctcccctggt cgtacgcgaa gccggacgtg agctcgtagg
    11821 gcgttccgcg gtcgtccacg atcacgaaga tcgagtgcgc gttgtcgagc cgggtgaggt
    11881 cgatgccgct cgaatcggag agcacgagcg tgacgtggct ccctggatgg aggagcgagt
    11941 cgggccccga gggatagacg acctggatgg tcggcggcgt cgcgtccacg cgcggcgagg
    12001 aggcggccgc gatccggagc gagtccaccg ccagcccgac gccacgccct ccagcctggg
    12061 acaggagcgc ccggagcttt ccaccacgcc ccacgagccg tccgtcggtc ggcacgacga
    12121 acgtaacctc gaaggagccg tgatcgagcg gcacggtgcc gcggaatatc gtggggccgg
    12181 ggatctggta cgtctggggg gttccgctca cctgcgcgaa ctgcgaccga atgaacggag
    12241 gcccctggag gaggagatcg gcgttgccca tcgagacctg gtctcgtgtg gaatccgccg
    12301 gacccatcac cgcgtggccg cgcaaggtca cgacgtcccc gcgaaggact gaatcgagcg
    12361 gctgcttctc ccacacgccg gcgccgcgtg ggaggggcgg ccggagggcc ggatcgccga
    12421 ggaagccgta cttccgaacc gtgtcgttgc tcgggcccgc gagctcattc ttggcgcgcg
    12481 caaaggcaag ccccgcggtc ctcagcgtgt ccacgcgcgg cgcgaacggg aagagctcgt
    12541 cgaggaagct ctcgttcaga ggaccgctgc tcgacggaag ggtgagcccg ctcgccgcaa
    12601 gcgaagcgac tgcgccgcct ccggggagcg tgagaaggag ctcgccgaga ccttccccgc
    12661 tcggctcgtc gaacttcccg accgtgcagc tgaagtatcc gaagatcgac gggcgcgtcg
    12721 cgttcgtgag acgcgtcgcg tcggccgaac ggaagacctg ctcgtccgcg agcacctggt
    12781 ccgagccgtg gccgacgtag ttcgtgagcc acgcgccgcg attgatctgg gcgatgaact
    12841 cctcgcgcgc ggcgggcttc gactggtgcg tcgtgtcgga gaaggcgaaa tcgttcaagt
    12901 acaccttcgc ccgctcgacc ggatacggca ggtggagccc atccaaacgc tccgactggc
    12961 gcacgtgttc gttccccacc gggtcgatct cgtccctttg caccgcgtcg tccgcgcaca
    13021 ggatcccgcg ggcgcgccac atgtcgaagt cctggctctc ctcgtacagg cgcagcttgc
    13081 cggcggtcag ggtttgcgcc tcggcggtcg tgcccgccgg gagccgcccg accgcgagat
    13141 ccaggaggag atcccccggc ccgtcgagga ttccgtagaa gtcgtccgag ttgtactgct
    13201 gggtctggct gacgtcgtag tagttgctat aggtgggaac cagatccggc gtcccgaatc
    13261 cgatgtaatt cttcgggtcg aacgtcgcgt cgccgaggag gcagagatag cttggcggca
    13321 cgccggtcca gtgcgcggcc gcgtactgca tgagattccg gatcgccacg gggctcggca
    13381 gccccgcccc gagctgcgcg aacacacgct ccgtggtggc gatgcgcacc cgcggcgcgg
    13441 gaatgccttc gatgccgagc gaacggtagg ctacgagcgc ctcggcctgg gcgaggaacg
    13501 cagggggcgc gacgatcacg tagtccgcct cgttcgatgg atcgaggagg tccgagatcg
    13561 tgttcgcgct cgaggtgggc gcgtagaccg agatccccgg cgtcgccgcg cgcgtagtcg
    13621 agacgagcga gtagcgcggc cggtatccat cgccggcgct gtcctccacg gtccacgcga
    13681 acggcgccgc gccgcccgag acgaatcccc ccacgagccg aaccgggctc tcgggatcgg
    13741 tccggtcgag gagccagacc gcggacgtgt cgctgatgcc ggtgatctgg tagcgaaccg
    13801 gtgcattggg aagtgaatcg ggagcggcga actggagggt gtcgttgacc gcgaggagct
    13861 gccgcggata gccgatctcg aaccacgcaa gataggagcg gtccgagcgc tgcgggaacg
    13921 gccccggatc gatgagcgcg ggcacctcga cgtcgagcgt gtcgcgcccc tgggtgatca
    13981 cccccgtcgc cgcgagatcc tggtgcaggg acagatccca tcccgccgtg tccacgagtg
    14041 cgcgattcca gtagatccgc gcgacgtgat cggggagtcc ggcgccgatc gagcttccga
    14101 cgccccacac gcggaccagc acgtcggcgc ttcccccagg ctgggccccc ggcatcgtga
    14161 gatcgaagcg aacgcgcgag ccctggaaca ggatccggtt gaaccatcgc tcccagcgca
    14221 cccccggctc gaagagatcc gcgttgtaga gccggttctc ctcgtagtgc acgcggtgcg
    14281 gcacggccag gtaggtcggc gctgccaccg cgtcgagcgc ggcgctccgc gtcgccatgc
    14341 ggcgcggcgg cgtggcgacc ggacccggcc cccatgtcat ccagagggtc tcgacgtcgg
    14401 agtgctgggt ctcctgatag gcggggccgc cgccgaacac caggtcgtat ccgaatccgg
    14461 tcgatccggt cgcgtacacg tagatccgat ccgcgggatc gagcgacccg tctccagcac
    14521 ccgtcacttc gatcgcgcac tcgcgaagcg aatccggccc ctgatcgacg ttcacgttct
    14581 ccgggaggtc gcctggggtc gagcggaaga tccggatcgt ggccggatcg atcgccccga
    14641 cgccgacacc ggcggcggcg agatcggaag ccgtcacccg gtagaggcct gggcggagca
    14701 cgttgagccg cacccaggtc cgcaagggat cgggtgtgac gcggagcggt ccgatcgcgg
    14761 ccgcacggcc tgcgagcgac cgtgccccgc gcgcgggacc cgcggacgcg ggcgcggtcc
    14821 gcggagcccg tgaaggatag taaggaccga ccgcgccgga cggcgccgtg gaggcgcgcg
    14881 ccgaggccgg acccgcgagc ggctccacgg gaccatccca ggtgacctcg atcgcgacct
    14941 cgtccacgaa gctcgcgctc ccggacgaag catcccagga cacgggacgg atggtgaccg
    15001 cgaggagccg ctcgccccga gctgccatga ctcgcgttgt cgccagtccc tgcacgctcg
    15061 gggcgcgcca tgacccttgc gcctggagcg ctgcctcgag gtcggccgcg gtgacgcgct
    15121 cgcgcatcga agcgtcggtg aggaggtacg ggaagggcgc cgggcgaagc gtgccgagcg
    15181 atcgcgccgg cgacggaacc acggacgcgc gcacgcgcgc gccccacgga atgcgaagga
    15241 agacggtgcg cgcgggaaga ggcggagcgc cgggtgcggc gcgctcgggg accgcgcccg
    15301 ggacgtcgat ctccgcgagc ggcgtgccca ggaactcgcc ccggacgacc gaaggctcgc
    15361 ccagcgtgac gcggaccttg tagccggtcg gctgcacgga ggagacgacg ggagcgggag
    15421 ccgaggccgc caaggagcca cgcgccagaa gcgggagcgc cgtccccagc gcgaggacgg
    15481 cggcgacggc atgcgttcta cggatcaacc ccactagatc cgctcggctt cctcgatcag
    15541 gaaattgggg acgccgtcga cgatcgaata ccggagacgg cagtggtggc acgtgaacgt
    15601 ccccgcggcg cggtcgtact cgaggtcctg gcggcactga ggacacacga ggatcgcgag
    15661 gagctgatcg ctgatgggcg cgccggcgcc ttcggcggcg gggcgggctg gctctcggtc
    15721 cttctcgggc atggcgttgg ctcctcgtca gccttcctgc gtggctccgg cggcggcggt
    15781 ttcctccagg tacaccccca tcttcaaata cttctccagc cttcgatcca ggagctcctg
    15841 tttcggaagg ccgatcagcg ccttgaggtg acggacgacg gtctccttga cccggcgcgc
    15901 ggtctcttcg ggatcccgat gggcgccccc gagcggctcg gggatgacct cgtccacgac
    15961 cttgagctcg agcaggtcct gggcggtgag tctcaggttc tcggccgcct gctccgcctt
    16021 gctcgcgtcc ttccagagga tggccgcgca gccttcgggc gtgatcaccg aatagatcga
    16081 gttctcgagc atcatcacca cgtccccgac cgcgagcgcg agcgcgcccc ccgatccccc
    16141 ttcgccgatc acgacggtca cgatcggcac gggaagatgc gccatttcga gaatgttccg
    16201 cgcgagcgcc tccgactgtc cccgctcctc ggcgccgagc ccagggtagg cgcccggcgt
    16261 gtcgacgaag gttacgatcg gaaatccgaa gttcgccgcc atgcgcatca gtcgaagcgc
    16321 cttccggtag ccctcgggat tcgccatgcc gaagttgcgg tggatgttct ccttggtgtc
    16381 gcgccctttc tggtgcccca ggaagaggac cggcagctcg tcgagcatgc cgagcccgcc
    16441 cacgatcgcg ggatcgtcgg cgaagcgtcg atcgccgtgg agctccatga actgcggcgc
    16501 catgaccttc aagtagtcga gcgtgtaggg gcggcgcggg tggcgcgcga gcttcacgcg
    16561 ctgccagcgc gtgagcttgg agaagatgtc cttccggagc cggtcggcct tcttctcgag
    16621 gcggtcgagc tcttccgcgg tctcgcgctc gtcgcctccg gcgcggctct tcagctcctc
    16681 gatcttccgc tcgagctcga tgatcggctt ttcgaaatcg agccaggttc cgttcatgcg
    16741 gctccactca tcggggtgac ggcgacctcc tccgcgcgct ccacgacgcg caccgcctcc
    16801 gcgcccaggc gctcccgaag agcgcccagg agctcctcgt tcggcgagac caggaggcgc
    16861 ttggcccgga tctgcgtcgg cccggggtcg gtctcgccgc gaaccgtgaa gtatacgggg
    16921 ctgatgccgg gatggcgaac gaggagggcg cggatctcct cgagggaaac cttctcaccg
    16981 ccccgaacca ggtcgatgtg gacggcggcg ccggacgggg gcggccccag aggcttcacc
    17041 gcggtgagca ccatcttcgg ctccgcgtcc tccctgagat tgacccggcc gcggacttcg
    17101 agcaccgcgc ccggctcgag atcggcgcga tgcttctcga agagatcggc gaacaccgtg
    17161 gtctcgacgg tccccttgag atcctcgagc gtgacgaagg ccattggacg gccgttccgg
    17221 tcggtgtggg ccttccgcgc ctggacgaga cagacgagcg agacctcggc caggtcgcgc
    17281 gcctcgcgca ggcgcgagat ctcggtcgtc gcgcgcgcct cgatgtaggg ccggtacggc
    17341 gtgagcggat ggtcggagaa gtagaacccg agcacctccc gctcgcggcg gagacgctcg
    17401 tcgaggggcc agggatcgac cttgggcagc ggcgggtcgg gcgcgacgat ctcgtgctcg
    17461 gccccgaaga gactctcctg gttcgcgttc acggctcgcg cgcgctcctg agcctgcgcg
    17521 aggagatcgc cgaccgcggc caggagctgc gcgcgatccc cctcgagcga atcgcaggcg
    17581 ccggcctgga ccagcgagtc gagcacgcgg cgattgatgg ccgcgagatc gacgcgccgg
    17641 atgaagtccg cgagcgttcg gaagtggccc ccttcgtggc gcgcgcggac gatcgcttcg
    17701 accgatccct gccccacgtt cttcaccgcg gcgagcccgt agcggatcgc cgggccctcc
    17761 agggtgaagg atgcgacgct cgcgttgacg tccggcggga ggaccgcgat gccgagccgc
    17821 cgcgcctcct ccaccaaggt cacgattcgg tccgtgtccc ccgcctcgct cgtgagcgac
    17881 gccgcgaggt aggccgcggc atggtgtgcc ttgagccatg cggattggta cgagagcatc
    17941 gcgtaggcgg cgctgtggga gcgcacgaag ccgtagcccg cgaacttgtc catgagatcg
    18001 aagatctctt cggccttctt tcccgggacg ttgttcttcg cgcagccttc catgaaggcg
    18061 gtcttctggc gcgccatctc ctccggcttc ttcttcccca tcgcgcggcg gaggagatcc
    18121 gcctgggcga ggctgaagcc cgcgaggcgg ttcgcgatct gcatcacctg gtcctggtac
    18181 acgatgacgc cgtaggtctt cttgaggagc gtctcgagct gcggatgcat gtacgtcacc
    18241 ttctgcttgc cgtgcttccg cttcgcgaag tcgtcgagca tgccgctctg gatgggcccg
    18301 ggccggaaga gcgcgttgat cgcgatgatg tcctcgaacg tgtcggggag caccttccgg
    18361 aggagatcgc gcatcccgcc cgattccagc tggaagatgc cgaccgtctg cgccttctgg
    18421 aggagcgcgt acgtatcggg gtcgtcgagc ggcagggagc cgagatcgat ggcgtctccg
    18481 gtcgcctcgc gcgcgagctc gaccgcctgc tcgaggacag tcagcgtgcg gagaccgagg
    18541 aagtccatct tgaggagtcc cacccgctcg agcgacttca tgtcgaactg ggtcgtgaag
    18601 tcccccttcg gcgaacggta catcggcacg tgctcggtca gattgcccgg ggcgatcaag
    18661 atccccgccg cgtgcgtgga ggcgtgccgc gccagcccct cgagcgccat ggagcagcgg
    18721 aggagccgcg cgtggcgttc gtcctggagg agcgccttca gctccggcac cgtctcgagc
    18781 gcgcgctgga gcgtcatccc gagatccggc gggatcatct tcgcgacctt gtctccttcc
    18841 gcgaaggtca tcccgagcgc gcgcgccacg tcgcgcacca ccgcgcgcgc cgccatcgtt
    18901 ccgaaggtga tgatctggct cacgctgtcg cggccgtact tcccgaccac gtagtcgatg
    18961 accttgccgc ggtcctcgta cgcgaagtcg atgtcgatat ccggcatgct cacgcgctcc
    19021 ggattcagga agcgctcgaa gaggagatcg aacttgatcg gatcgatgtc ggtgatggcg
    19081 agggtgtagg agacgaggct ccccgccgcc gagccgcgcc cggggccgac gcccacgccg
    19141 ttctcgcgcg cgtagtggat gaagtcgcgc acgatcagga agtagcgcgc gtagcccacc
    19201 tggcagatgg tgtcgagctc gtactcgagg cgcttctcga tctcttcggt gattcctccc
    19261 gggtaccgct ggtcgaggcc ctcgcgcgcc aggcgccgca ggtacacctc ctcgctctcg
    19321 aactcgggcg ggatcgggaa ggacggaagc tgcacgcgtt cggtgtcgag cgtcagctcg
    19381 caccgctccg ccacgacgag cgtgttggtg agcgcctccg gcgtctcgcc gaagagcgcc
    19441 tccatctcgt cgggcgtctt cacgtagagc tggtcggtcg tgaatcggaa ccggtccggg
    19501 tccagcatcg tcttcccggt ctggatgcag aggagcactt cgtgcgcctc ggcgtcctcg
    19561 cgcgcgaggt agtggcagtc gttcgtcgcc acgaccggta tgcccatcga gcgggacagc
    19621 tcgatcagcc cgcgcgccgc cttcttctcc tcctcgagcc cgtggtcctg gatctcgaag
    19681 aagtagaact ccggcccgaa gaggtcgcga taccagccgg ccgccttttg cgccccctcc
    19741 atgtcatcgg ccaccacgag ctgcggcacc tcgccccgaa ggcaggcgct caggcagagg
    19801 agcccgcccg cgtgctcggc caggagctcc ttgtcgatgc gcggcttgta gtggaagcct
    19861 tcgagatacc cggcggagga gagcttggtc aggttccgat agccctcgcg gtttcgcgcg
    19921 aggaggacca gatgatcgta ggcgccgcgt ccatcggccg tgcgcatctt gtcggcgcgc
    19981 gatccgtggg cgacgtagac ctcgcatccg atgatcggct tgacccccgc ggcgtgggcg
    20041 gcgtcgtaga actcgaccgc gccgaagaga tttccatgat ccgtgagcgc gagtgcgggc
    20101 atcttcatcg ccgcggcgcg cgcgatcaga cggtcgaggc ggcacgcgcc gtccaggagc
    20161 gagtagtcgc tgtggttatg taggtggacg aagggttggc tcatcggagg ctcacgcggc
    20221 cccatcgacg cagactgagg tcagccggaa gcggggcaca tccgcagtct acacgagggg
    20281 cttccccacc cctacgaatt tccgttcgcg cggagccgcg cgatgtgtag gatgcgctcg
    20341 tgacctccgt ctcatccgcc gggccggacc tcgtccaagc ccgcgcgcgt ctgcgcgcga
    20401 tcctcctcga gcgatccatc cgcttcggcg acttcacgct ctcttcggga gcgaagagcc
    20461 gctactacgc cgacgtccgg cgcacgagcc tccaccccga gggcgcgctc ctcatcgcgc
    20521 agctcctcga tccgattctc caagagcacg gcgtggcgtc gatcggagga ctcacgctgg
    20581 gcgcggatcc catcgtgggc gcgacgatcg cgtggacgca gctccaagga cggggcctcg
    20641 tcggattcct cgtgcgcaag gagcagaagg cgcacggcat ggggaatcgc atcgagggac
    20701 cgttcgatcc ctcgctcccg gtcgcgatcg tggacgacgt gatcacgaag ggaggatcgg
    20761 ccctgaccgc gtatctcgcg gcgaaggagg ccggagcgga cgtgcgcgtg gtcgcctgcg
    20821 tcgtggatcg aaacgaagga gggggcgagg agttcgcgaa gctcggcgtt cccttccggc
    20881 cgctctttcg aatcgacgag ttcctctagc gtcgaagcgc gcgcccgcgc gctccacccg
    20941 acagcctaga gtccctggcc tccctgatcg tcgggaggcg gcgtctcctc gtccggaggc
    21001 tgctcgggct gctggttttc cttctgcgtg tccggggtgc ccgtggaatc ggacatgaag
    21061 ccccccaggc tgtcgggcca cacgaccggg tggaagaccg ggcagacctg acgcggctcc
    21121 gtcttccgga tgaaggcggc ctgcatcacg tgagggcagc cggggccggc gcggtagccg
    21181 gtgtcgccat cgatccagac gtccaccacg tcggggggcg gctcgaagtc gcgtagggga
    21241 cgatcgcggt gcgccacgtc catgaagcgc gcccagatgg ggacggccgc ctgggtcccg
    21301 gtcaaaccga gccttcggtt cttgtcgcag cccacccaca cgccggtcgc gaggtcgggc
    21361 gtgaaaccca cgaaccacgc gtcgttctcg tcatcggtgg tcccggtctt ccccgccgcc
    21421 ggccgcacga atccgtactc ggtccggacg cccgcggcgg tgccgtagat caccggcccc
    21481 tcgagcagga tggtcgccat gtacgccgtt tcgggatgga gcacccggcg gctctcgcgc
    21541 ttcggcgacc agcgcaccga tccgctccgg tcgagcaccg ccttgaccgg tgtcggctcc
    21601 accaggacgc cgccgttcgc gatcgtggcg tacgccgtcg tcagctcgag caccgagagc
    21661 tcgctcgtgc cgagcgcgag cgacgcgacg gcgcggagcc ggctctggat gcctaaggag
    21721 cgcgccacat ccacgacccg gttcatgccg accatctgcg cgaggtggac cgtggccacg
    21781 ttgagcgagc gcgcgatcgc gcgggccgcg gtgacacgac cggcgtagga gttctcgaag
    21841 ttgcgcggcg cccacggccc cagatccgtc tggaaggtgt cgggcgcatc ctcgaggacc
    21901 gtggccgcgg tgagcggcgg acggcgcccc gcgaagtagg aatcgagcgc ggcgatgtag
    21961 acgaacggct tgaacgcgga gcctggctgg cgcctcgagt cgaccgcgcg gttgaacggg
    22021 ctctcggcgt agctccgccc tccgatcatg gcacggatgt tccctgtcga gggctcgatc
    22081 gcgatgagcg caccctgcgc cggatcgcgc tcggcgccgc cggacggcga tcgccagtcc
    22141 gcctccttgg cgccgttccg gaggatcttc tccccctcct cctgcaggcc cgagtcgagc
    22201 gtcgtgaaga tggtgaagcc gcgcccggag agctcgctct cggggatgtc cttggagagc
    22261 gtctcctgca cgtagtcgac gaagtacggc gcgagtgtgg ccggaggcgg gttcgcggcg
    22321 aagcgcatct gcgtgcggcg gtacttccgt gcttcctgct tcgagatgta gccggcctcg
    22381 gccatgtcct ggagcacttc gttccgccgg acgatggcag cctcggcgtc acggtaaggc
    22441 gagaaccggt tcggcgcggg gatgatcccc acgatgagcg ccgcctcggg cacggtcagc
    22501 tcctgcgcgt gtttgttgaa gtagtagagc gacgcctcct cgacgccgca cacgctccat
    22561 gacccgcgct gcccgaggta gatctggttg agatagaact cgaggatctg gtccttggag
    22621 tagcgaagct cgaccaggac cgcgaggagc gcctcgtgga attttctcca aatgctccgc
    22681 tcgtgggtca ggaagaagtt cttcacgagc tgctgggtga tcgtgctccc gccttcgacc
    22741 accgtgcgcg cacgcacgtt ccgcatcgtc gcgcgtccca tgccgaggag gtcgagcccc
    22801 cagtgatggt agaagcgccg gtcctcgctc gcgatggtcg cgaggatgag atgccgcggc
    22861 aggcggtcga gcggcacgta gctccgccgc tcgcgcgcgg gatcggcgaa ctcgccgagg
    22921 agctccggct cgatgcgaag gagccctcgt ccttccgcat ttccgccgaa gccattgccg
    22981 agcgactcga cccgtccgtt ctggaggcgc acggtcacgg gcgttccgtg ctccacgcgg
    23041 tcgggatagg tgaacgcccg gagatagact tcgaagatcg gcccgttcac gcgataccgg
    23101 ccaggatccg ccgggcgctt cgtgacgcgc tgatagtgcg cgcgatcgag gaggcgctgg
    23161 agcgtggacg actggagcgc gtcgccgact cggtagtcct tccagtcggc gtacacccgc
    23221 gtcggaaatt tccagccctg ccccacgtag cgcgacgatc cgatcgagga gagccggaac
    23281 cagtcgtacc cgaggaagca gaacgcgacg gccgcgagga agaggagcca gcgaagtgtt
    23341 ccgggcggag cccacgacgg acgccaggac gaccatgaag gaggccagga gggacgccat
    23401 tcccaccaac gctttccgct cccgggcttg ccgggtgggg gcttggcggg agtggtctgt
    23461 cgtgcgggac gcggtttggg cggacgccgc ggctcgctgg gttcggtccg atccatggag
    23521 ttgaaggacg agcctagggg tccgtgaggg aggcgtcaat cggccgcgcc cgacgccggg
    23581 ggcggcacgg agctactgcg agtcgctcag aacgtcttcc agatgccgca tccgggccac
    23641 cgagcgcgcg tcgacgccgc cggcgggcgc gtggagcacg tactcgcgat aggcggccaa
    23701 ggcttccttc cgatcggcaa ccctccccgt gatctcctgg atcgcccaga ggcagtcggc
    23761 gtactcgcgc ttcgaccgct cgcgctcggt accgagcgcg gtccggaggt aggacttcca
    23821 ggacgccttg atcttccgga gcgcttcgat gtcccgatct tcgtgcgcct tcacggtcgc
    23881 gagatggagg cgccccaccg ggccgctcgt gtcggcctcg tccggagggg tcgccggacg
    23941 cacctcctcg ggtggctgct cgacgatgaa cgagggcttc ttggtggtcg cgggcggctt
    24001 cttcgtcgtc ggcgccggtg tgaaatcggt ctgtggccac gtccccttcg tgtcggccgg
    24061 accgtccacg accggctcga atccctcctc gcgcgtcggc gggccgcgcc gtggaggaac
    24121 ggtgcccggg ggcgccgtcg tttccgtcgt gggcgcgtcg tgggggggct tggagctgga
    24181 cgcgatcgac ttggcgcccg gcttcgtcgc ggcgccagcc gcggtcgtgc tgggctgggg
    24241 cttcgtcggc ttgctggcgg cggtcgcgtt cggcgcggcc gaaccggtgt gggtcgcgcc
    24301 gggctgcacg gtccccgact gcgccgcgcc gggctgcgac ggcgccgtcc cggagggcgc
    24361 gccgatcgtg ggcgtgaccg gcgtctgtgc cgcgccgatc gcgatcgaat ccttcatcgc
    24421 cgtgcttccc accgaggcgc tatccgcggc tccatgcgga gggggagcga gcgcctgcgg
    24481 gtcgttggcc gtctcgcgat gcgccatggc catccagacc gacaccgcaa gcagcgccac
    24541 gcccgcgagc gcaccgcagc tccagagcac gatgcggaag accgcgcgcg agccctcgcg
    24601 ttggctcgag cggttgagcg cctcctcggc gaaattgagg ctctcttcgg gagggctcga
    24661 ggtgcgcggc ggatcgaccg gctcgaaatc ccaggccttc ttggagggca tgggaggaat
    24721 gagcgatggc cgctccccca tcgaagacgt ttccggcttg ggcggcgccg tgggcagagc
    24781 cttggactcg agcgcgagat ccgcaaagga ggcttcggga tccggcgccg tggacaaggt
    24841 cggcgtgatg ggctcgagca cgagacccgg ctcgacggcc gggggcgcga gcgtgggggg
    24901 cagaggcggc gagaccgggt cggcgaatcc ggccaccggt ccaaccgacg gtgggggagc
    24961 cggcgaatcg gtggtaggcg tcgcttcccc cgacgtcccg tggatcgcct ggagcagatg
    25021 gtgctccagg cgacagccct cgcaacttgc gagatgagac tcggtttcgg ccttttcttc
    25081 aggcgtcaag aggcccgaga cgagcccgct cagcctcgat ttgacggtcg cgcagtccac
    25141 gaagcgtgga acccccacgt tcaaggtcct cggcccaaag ggccgcgaca cggcatatga
    25201 atccgcaccg agttatcggt ataacgagcg gggaccttga gggattggcg ggccccgttc
    25261 ggggcccggc cgggggcttc aatcggggag gatggctccc tcgacggggc aggcttccag
    25321 gcaggcgtta cagctcacgc agtcctcggc gacgatcagg taccagcccg tggctttggc
    25381 gttctcgccg tcgcccttct tgatgcagtc gacgggacag acgtccacgc aggagctctc
    25441 cccctcgcag atgtccttca cgatttcgta agccatgctt cctccgagcc tcctggctcc
    25501 gttcgagggc cccgggccgc tagtggacct ggagcatgcg gtcgagcgcg acgtgcgccc
    25561 accgcttcgt cgtctccggc acgcggatca cgttcacagg acgcccctcg acgatgctgt
    25621 cgagcgccca agccaggttt tgcggcgaga tgcgatacat cgtagcgcag agacagaatt
    25681 ccttcgagag gatctcgatc ttcttgtccg gcatcgaatg cgcgagccgc tgcacgaggt
    25741 gatactcggt tccgaccgcc cagtgcgatc cggccggcgc ctcgcgcagg gtccggatga
    25801 tcgtctcggt cgagccgacg gcgtccgacg ccgcgacgac ctcgtgcggg acttccggat
    25861 ggaccaggat cttcacctcc gggtcctccg cgcggcggtc gagcacgtgc tgcaagtgga
    25921 acttggtgtg cacggagcag catcccttcc acacgatgac cttggcgcgg cggagctcat
    25981 cctcggtgga tccgccgagg ggacgcttgg gatcccacac gacgatccgc tccggtgcga
    26041 tgcccatcgc gatggccgtg ttccgtccca ggtgctcgtc cggcaagaag aagatctgat
    26101 cccgctgcgc gaaggaccat tcgaagatcg cgcggcagtt ggacgaggtg cagacggccc
    26161 ctccccgctc gccgcagtac gctttgatct cggcggtcga gttcatgtag gtcacgggca
    26221 tgactgacga gagaccgagc cgctcgagcg cggcgcccgc ctcgatcacg tcgtcggtcg
    26281 gcgccatgtc ggccatcgag cagccggcgt tgagatcggg caggacgacg gtctggtcgg
    26341 gctggcgcag gatgtccgcg ctctccgcca tgaagtggac gccgcagaag acgatccact
    26401 tcgcgtccgt gcgctccgcg ccgagccggg ccagcttgta cgagtcgccg gtgatgtcgg
    26461 cgtgctggat gacgtcgtcc ctctggtaat ggtgtcccag caccacgagc gagcttccga
    26521 ggcgcgcctt ggcctcgcgg atgcgcgacg ccagggcggc gtcgtccagc tcgaggtagg
    26581 gctcggggac cgtgaggacg cgggggctca tagctgtcat ctttgatgcg gaaacgggcc
    26641 cggtcaagcg aaagaaccgt gagcccggac gcgaatcgtc cgcgcatctg ttgcgtctaa
    26701 acagagttgg agccgcgccg accatgcagc ggtcttgacc ctcccgcctc gaaatgggac
    26761 catggccctt ccggcgcggg ccggcgcacc gaaccctcga caccgggacg agctttgtca
    26821 gccatcgaca accgattcac gatccacgtc gcgaccgtca acggctcggg aagccagacg
    26881 gccaacacga cccttcttcg cgcgctcttc cagatgggga ttcccgtctc gggtaagaac
    26941 ctcttcccct ccaacatcgc gggactcccc acctggttca ccattcgcgc gaacgaccag
    27001 gggtacgtcg cgcgcatcaa gcgcgccgat ctgctggtcg cattgaaccc cgagacgtcg
    27061 gtggccgacg tcgccgcgat ggagccgggg agcgtgctcg tcgtagccga cggcctcccg
    27121 atgccggcgg ttccccccga tcgcacgctc tacaaggttc cattcacgaa gctggtcgag
    27181 cccctcgtcg ccgacgcgaa gctccggaag ctcgtcgtga acatgatcta cgtcggcgtc
    27241 gtgagctggc tcctgggcat cgatcccgac gaggtcgacc gcgcgctcgt cgcgcagctc
    27301 gggaggaagc ctaaggcgct cgagatgaac cgcgccgccg tccgggtggg aatcgagtat
    27361 gccgagaagc acatgatgcg gacgcccttc caggctcggc gcgcggacag gaccgcgggc
    27421 aagatcctga tcgacggcaa ccaggccgcc gcgctcggtg ccctgttcgg gggcgtgacc
    27481 ttcctctcgt ggtatcccat cacgccgtcg tcgagcctcg cggagcagct cgagggattt
    27541 ctcgcgcggt accggaaaga tcccaagagc ggcaaggcga cctacgccgt gatccaggcc
    27601 gaggacgaga tctccgcgat cggcatggtg ctcggcgcgt cgtgggcggg cgcgcgcgcc
    27661 atgaccacga cctcgggacc cgggctttcc ctcatgaccg aattcacggg ctacgcctac
    27721 tacgccgaga tccccgcggt gatctgggac atccagcgga tcggccccag cacggggttg
    27781 ccgacccgga cgtcgcaggc ggacatcctc tcggtcgcgt tcctgtcgca cggggacacg
    27841 aagcacgtca ttctcttccc cgcaaccgtg caggactgct acgagtacgg catgcgcgcg
    27901 ttcgacctgg ccgagcggct ccagacgccc gtgttcgttc tctccgatct cgacatcggg
    27961 atgaacacct ggatgtccca gcccttccgc taccccgaga atccgtggga ccgcgggaag
    28021 gttctgtccg cctcggacct cgagcgcgtg aagaagttcg agcgctaccg ggacgtcgac
    28081 ggggacgcga tcccgtaccg cacgcttccc ggcaccaacc atccgctggc cgcctacttc
    28141 acgcgcggct cggggcacga tgaagcggcc cgttacacgg agagctcgga agcctatccg
    28201 cgcgtcatgg accgcctcgc ccggaagttc gagaccgcga aaacgctcgt gcccgcgccg
    28261 ttcgtcgaga agacgcaggg cgccgacgtg ggcctcatcg ccttcggctc gacgcatcac
    28321 gcgatggacg agacgcggga caagctgcgc accgaaggca cgccgacgtc ctacctgctc
    28381 ctccgggcct atccgttccc ggaggaagtc gagcgcttcg tgcgtggaca caagcgagtc
    28441 tacgtcgtgg agcaaaaccg ggacgcgcag atggccgccc tccttcgaat ggagtacccg
    28501 gagctggcgc cgcgcattcg ctcggtgctc cactatgacg gccttccgat cgacgccgag
    28561 agcatcctga acggcgtccg gtccgccgag cggccgtcgg aggtgacgac ccgatgagca
    28621 ccccgccgaa cgtcaaccgg atcggcctca ccgcggccga ctacaagggc tcgaagtcga
    28681 ctctatgtcc gggctgcggg cacgacgcga tcaccggcag catcatccag gcggcctacg
    28741 agctggggct cgagcagcac aaggtggcca agatgtcggg catcggctgc tccagcaaga
    28801 cgccggccta cttcctcggc cgctcgcact cgttcaacgc ggtgcacggc cgcatgccgt
    28861 cggtggcgac cggcgcgcac ctcgcgaacc gcgagctgat catgatgggg gtctcgggcg
    28921 acggcgacag cgcgtccatc gggctcggcc agttctgcca tctcgtgcgg cgaaacgtgc
    28981 cgatcgtcta catcatcgag aacaacggcg tgtacgggct cacgaaaggg cagttctcgg
    29041 ccaccgccga cgtcgggacg cgtgcgaagt cgggccaggt gaacgagatg atgccgatcg
    29101 atctctgcga aatggcgatc cagctcggct gcggcttcgt ggcccgttcg ttctccgggg
    29161 atccgaagca gctccggcca ctattgaaag ccgccttctc gcaccgcggc acttcggtgc
    29221 tcgacgtgat cagcccctgc gtgacgttca acaaccacga gggctcgacc aagagctaca
    29281 cgagcgccaa ggagcacgac gcgccgctcc acgaagtgac gttcatcccg cacttcgaga
    29341 acatcgagat cgactacgcg cccggcgaga gccgcgaggt caccctccac gacggatcga
    29401 ggatcgtgct ccggaagctg gaggaggact acgatccgac cgaccgcatg cgggccatcg
    29461 atacgatcga gcggggacgc gtcgaggacc ggctcgcgac gggactcctc tatcagtcga
    29521 aggggaacgc cccgatcggc gaggacctgg ggctggtgga cgagccgctc gcgtccctgc
    29581 cgctcgagcg cgtgcgcccg ccgcgcgagg ttctggacaa gatcatggcg ggcctgatga
    29641 ccggcggagg cgcctagccc cgttcggccc gagcgcggac gggtcgctcc gcgccggcga
    29701 ttgcttccta ctcgtctccc ccgtcgtcct cgccccgtgt gttccggtag tataccttga
    29761 acttcttccg gagccggttc tgccgcacgc gctcgcgcca cgcgcggatg ggattccgcc
    29821 agcggaacga gaccccgcgc cccgagcccc gtccaaagcg cctcatgtag agccagccga
    29881 agaggagccc gcccaggtgc gccacgttcg cgacgccgcc gccggtggcg gtcagcgtgg
    29941 cccagaacgc gagcgctccg aggatgagca cgaaatactt cgccttgatc gggatgacga
    30001 agtagaggag cagcgtccgg tccggaaaga ggatgccgta cgcggccagg atcccgtaga
    30061 tcgcgcccga ggcgccgatg atcgggatcg tggagttggg cgtgacgagg atcgagcaga
    30121 gcgccgcgcc gatccccgtg atgaagtaga accgcacgaa ccgctgcgtc ccccagagcc
    30181 actccagctc gctcccgaac atccagagcc cgagcatgtt gaagaggatg tggaagaagc
    30241 ccccgtgcag gaagagatac gtgcccacct gccagaggaa gagatggtgg accacctcgt
    30301 acgggacgag gccgaagagg cgctcgaggc gcacctgcgc caagaagccc gccaggagct
    30361 ggaagacgta gacccccacc gtgacgaaca cgagcgtccg gacgcctggg gcgagcggac
    30421 cgccaaagac gatgggacgc ggctcgcgcc agctcacgcg cgcttctccg gcgccgggag
    30481 cttgggcccg aggtagaggc ggcgctcctc ggccgtgtcg tcgaacgcct gacggattcg
    30541 agagtccagc tcgacactga gatcgacctt gcggcgctcc atggctcgga cggcggtttc
    30601 gatcattgcg tcgatcctgt gctctgcgag cgaggacggc gtgccccaca cgaaggacgg
    30661 cacgtacacc ggccagagct cggcgccgta caggttgctg ccgatgccga gaacgcaccc
    30721 ggtcgggagc gacgtgctga tgccggtctt cgtgtagtcg ccgatcacgg ctccgagcag
    30781 cgtttgcccc gagccctcgg catggccgtc ggcataggcg cgggcggagc ggtagaagcg
    30841 aacctcgcca tagttgttct tgaggtcgct gttgttggtg ttcgcgccga gattgaccca
    30901 ttcgcccacg aacgagtgcc ccaggaagcc atcgtgctgc ttgttggcat atccctggaa
    30961 caccgacgcc tcgatctcgc ctcccacctt gcacacggga ccgatcgacg ttccgccgcg
    31021 gatcctcgcg ccggcccgta ccacggagcc ctcgccgatg acggccggcc cctcgatgta
    31081 ggcgagcggc tcgaccttcg cgttctcgcg gatcaggatc ggaccccctt ccgcgttcaa
    31141 ggcggcgccc gcctggacgc gcgcaccgcg cgccacgtgc accgcctcgg gctgcgtcac
    31201 gtggacgccc ggctcgatcg tgccccgcac cgtgcccggc tgaaagccat ggcggaaatc
    31261 ctggacgatc tgacgcgggc tccagcgaat gagatccgcc gcggaccgcg cgaggaaggc
    31321 gttcacttcc gcggggaagc gcaggtcctc gaacgcgcgc tccggatctc cctcgcggag
    31381 atagcgcgcc gcgtgcacca cgcgctccgc cttgaccttg gcggcgaaga gggtgccatc
    31441 ggccatgtac gacgcctcgc tcggcgacgc ggcgaggaaa tggaggatct cgtcgtccgt
    31501 catgcagagc cgcgcgttga cgaagagcgt ctcctcagcc gggggagcgc ccacgtgggc
    31561 cgtcggatag aggaggcgcg tcacctcctc gatctccgga cgcacgtgga gcacgagcgg
    31621 cgattcggga aacgcgcgca cgagtttttc cacaagggaa aacgcgccgc agcggagggc
    31681 gaaggcgggc cggagcagtg tgtggggtcc gaacgactgg acgagcgggt cctcgaagag
    31741 acaaaggttc acgagcaaaa ggtagggacg atccgcggtc gcgtcaagtc atggcgcctg
    31801 ggactccgag cgcctacaat gcccct
//
LOCUS       DASTKC010000166         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1313194, whole genome shotgun sequence.
ACCESSION   DASTKC010000166 DASTKC010000000
VERSION     DASTKC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313194"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFR25_06610"
     CDS             <1..600
                     /locus_tag="VFR25_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET9940755.1"
                     /translation="TPRERYHMEVWKRFNEKELTHSMAHYLMAISALSQNGGASVSAI
                     AERLGVSKAGVTSMLRTLKTRGLVDHERYGDVQLTKVGQRLAMRTERSRDVLTRFFVE
                     TLGVEPSAAEEDACMIEHLVSVSSMVELLRMTAFLASDDPAAERFRTAFRDYQSHCRA
                     GSAGEGCPVCRGHCLRNTIEDVEAAVDEAQATLRAGGTA"
     gene            597..824
                     /locus_tag="VFR25_06615"
     CDS             597..824
                     /locus_tag="VFR25_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET9940756.1"
                     /translation="MRSLDRIVPGSRAVVQTIDAVDSFTRSLLELGLVPGTEIEVVRR
                     APLGDPIELVVRGSHFSIRRQEARRILVKAL"
     gene            808..2841
                     /gene="feoB"
                     /locus_tag="VFR25_06620"
     CDS             808..2841
                     /gene="feoB"
                     /locus_tag="VFR25_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET9940757.1"
                     /translation="MSKHSERIALVGSPNSGKSSLFNRLTGLRQKVANYPGVTVEKRA
                     GTCHLPSGRQVEILDLPGTYSLTPRSPDEALVRDVLEGRQGAAAHADRVVAVVDATRL
                     DRQLYLALQILRTRRPAMLVLNLMDEAEAQGIEIDVPAIERILGVPVLAVSAKTGMGV
                     DRLRQTIDRANGNGNGHAVSANGNGADGNGHGVEAGNGHAVAADAVWTELDPALGFKE
                     VESIVRQVVRRRPTEKAVRGRLDAILTHKILGPVIFVVLMATVFQSIYAWATPAMDLL
                     DAGVRALAGLGRATIPAGPIQSLLVDGILAGVGTVASFIPQLAILFFFIAVMEDTGYM
                     ARAAFLMDRVMGSVGLPGRAFLPLLSSFACAIPGIMATRTLPSRNDRLATILIAPLMS
                     CSARLPVYALLIGAFIPNRHFGILSLRGLTLLSMYFLGIVAALGVAWVARRTVLRGPR
                     TAHVLEMPPYRVPSARSVFLAIRERCLLFLGQAGSVILAVSIVLWFLASYPGGHPETK
                     ALESQAAAAAAAGHAGDAAKLRMDASGSALRHSFAGQLGHLIEPAIAPLGFDWKLGVG
                     LLSSIAAREVMVSTMATMNNLDGSADPSESLRQALPRAVNPKTGLPLYTPLVAVSLMV
                     FFALALQCMSTVAVAKRETNSWRWPIGMLVMMNVMAWVASLAVYQGGRMLGWG"
     gene            complement(3024..>3668)
                     /locus_tag="VFR25_06625"
     CDS             complement(3024..>3668)
                     /locus_tag="VFR25_06625"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9940758.1"
                     /translation="DGLVLQMAKSIQSGSTVFFPEQTIAAAVNLARKYQADERHGIKT
                     AEIAAAIFEATKQQHRMGDRERLLLEVASIVHDIGGFVAARGHHRHAYYLLVNSEIFG
                     LSGIDMEVVANVARYHRRAGPQADHPAYAALPRPARVTVNRLAAILRVADALDKGHNQ
                     ILRGVRVIVSGEELRIEGEGAGDLALERLSLEEKAGLFEEVFGLKPVLVEPAAS"
BASE COUNT          526 a         1281 c         1253 g          608 t
ORIGIN      
        1 accccgagag agcggtacca catggaagtt tggaagcgat tcaacgagaa agagctgacc
       61 cactcgatgg cgcactacct catggccatc tcggcgctga gccagaacgg aggcgcctcg
      121 gtgagcgcca tcgcggagcg cctcggcgtg agcaaggcgg gcgtgacctc gatgctgcgc
      181 acgctcaaga cccgcggcct cgtggatcac gagcgctacg gcgacgtaca gctcacgaaa
      241 gtgggccagc gtctcgccat gcgcacggag cggagccgcg acgtgctcac gcggttcttc
      301 gtcgagacgc tcggcgtgga gccttcggcg gccgaggaag acgcgtgcat gatcgagcac
      361 ctggtcagcg tttcctcgat ggtcgagctg cttcgcatga cggcgttcct cgcgtccgac
      421 gatccggccg ccgagcgctt ccgcaccgcc ttccgcgact accagagcca ctgccgcgca
      481 ggctcggcgg gcgaggggtg tcccgtgtgc cgcgggcact gtctcaggaa cacgatcgaa
      541 gacgtggagg cggcggtcga cgaagcgcag gccacgctcc gcgcgggagg cacggcgtga
      601 gatcgctcga ccgcatcgtt cccggctcgc gcgcggtggt gcagaccatc gatgccgtgg
      661 actcgttcac gcgctccctc ctcgagctgg gcctcgtccc cggaacggag atcgaggtcg
      721 tgcggcgcgc gccgctcgga gatcccatcg agctggtcgt gcgcgggtcc catttctcga
      781 tccggcgtca ggaggcgcgt cgcatccttg tcaaagcact ctgagcgaat cgccctggtc
      841 ggcagtccaa actcgggcaa atcctcgctg ttcaaccgcc tcaccggact caggcagaag
      901 gtagcgaact accccggcgt caccgtcgaa aagcgcgccg gcacgtgcca tctcccctcg
      961 gggcggcagg tcgagatcct cgatcttccg gggacgtaca gcctcacccc gcgctccccc
     1021 gatgaagccc tcgtgcgcga cgtgctcgag gggcgccaag gcgccgcggc gcacgccgat
     1081 cgcgtcgtcg ccgtcgtcga tgccacgcgc ctcgaccgcc agctctacct cgcgctccag
     1141 atcctccgga cacgccgccc cgcgatgctc gtgctgaatc tcatggacga ggccgaggcg
     1201 cagggcatcg agatcgacgt ccccgcgatc gagcggatcc tcggtgttcc ggtcctcgcg
     1261 gtctccgcga agacgggaat gggcgtggac cggctgcgcc agacgatcga tcgcgcgaac
     1321 ggcaacggga acgggcacgc cgtgagcgcc aacggaaacg gcgctgacgg caacggccac
     1381 ggcgtggaag ccggcaacgg ccacgcggtc gccgccgatg cggtgtggac cgagctcgat
     1441 cccgccctcg ggttcaagga agtcgagtcg atcgtgcgcc aggtggtccg ccggcgtccc
     1501 acggagaagg cggtccgcgg acggctcgac gcgatcctca cgcacaagat cctcggtccg
     1561 gtcatcttcg tggtcctgat ggcgaccgtg ttccagagca tttacgcctg ggccacgccg
     1621 gccatggacc tgctcgacgc gggcgtccgc gcgctcgcgg gactggggcg agcgacgatc
     1681 cccgcggggc cgatccagtc gctcctcgtc gacggcattc tcgcgggcgt gggcaccgtc
     1741 gcgtcgttca tcccgcagct cgcgatcctc ttcttcttca tcgcggtgat ggaagacacg
     1801 ggctacatgg cgcgtgccgc gttcttgatg gaccgtgtca tgggctcggt cgggctgccc
     1861 ggccgtgcgt tcctgccgtt gctctcgtcg ttcgcctgcg cgatcccagg gatcatggcc
     1921 acgaggaccc ttccgtcccg gaacgaccgc ctcgccacga tcctcatagc cccgctcatg
     1981 tcgtgcagcg cgcggctccc cgtgtacgcg ctcctgatcg gcgcgttcat ccccaaccgg
     2041 cactttggaa tcctgagtct gcgcggcctc acgcttttgt cgatgtactt cctcggaatc
     2101 gtggccgcgc tcggggtggc gtgggtcgcg cggcgcacgg tgcttcgcgg gccgcgcacg
     2161 gcgcacgttc tcgagatgcc gccctatcgc gtgccctcgg cgcgctcggt gttcctcgcg
     2221 atccgggagc gctgccttct cttcctgggc caggccggat ccgtgatcct cgcggtgtcg
     2281 atcgtgctct ggttcctcgc gtcgtatccg ggagggcatc ccgagacgaa ggcgctcgaa
     2341 tcccaggcgg ccgcggcggc ggccgcggga cacgccgggg acgcggcgaa gctccggatg
     2401 gacgcatcgg gctcggcgct ccgccattcc ttcgcggggc agctcggcca tctgatcgaa
     2461 cccgcgatcg cgccgctcgg cttcgactgg aagctgggcg tgggccttct ctcttcgatc
     2521 gcggcgcgcg aagtgatggt gtcgacgatg gcgaccatga acaacctcga cggctcggcc
     2581 gacccgtccg agtcgctccg ccaggcactc ccccgcgcgg tcaaccccaa gacggggctt
     2641 ccgctctaca cgcccctcgt cgcggtcagc ctcatggtct tcttcgcgct cgcgctccag
     2701 tgcatgtcca ccgtggcggt cgcgaagcgg gagacgaact cgtggcgatg gccgatcggc
     2761 atgctggtga tgatgaacgt gatggcgtgg gtggcctcac tcgccgtcta tcagggcggg
     2821 aggatgctcg gatgggggtg atccggtgag cgcgcctctc tggcagcacg ccgtggcggc
     2881 cgcgtgcgcg gtcgtggcgc tgggctggct cctgcgccgg tcgttgttcc gcggctcgcg
     2941 cgatgcggcg gatgagggtg tgaccgggcc gtgctcccac tgtccggcgc gcaaaggcgg
     3001 gccgatcgag cagaccgttc agctcaggaa gcggcaggct ccacgaggac cggcttaagc
     3061 ccgaatacct cttcgaagag tcctgccttc tcctcgagcg acagccgctc gagcgccagg
     3121 tccccagccc cctctccctc gatccgaagc tcctctcccg acacgatcac gcgcacgccg
     3181 cgcaggatct ggttgtgccc cttgtcgagc gcgtcagcga cgcggaggat ggcggccagg
     3241 cggttcaccg tgacccgagc cgggcgcggg agcgccgcgt aggcggggtg atccgcctgc
     3301 gggcccgcgc ggcggtgata gcgcgccacg ttcgccacga cctccatgtc gatccccgag
     3361 agaccgaaga tctccgagtt cacgagcagg tagtaggcgt ggcggtgatg gccgcgcgcc
     3421 gcgacgaagc cgccgatgtc gtggacgatg gaggcgacct cgaggaggag ccgctcccgg
     3481 tctcccatcc gatgctgctg cttcgtcgct tcgaagatgg cggcggcgat ctccgcggtc
     3541 ttgatgccgt gccgctcgtc ggcctggtac ttccgggcca ggttcaccgc cgccgcgatc
     3601 gtctgctccg ggaagaagac ggtcgacccg ctctggatcg acttggccat ctggaggacg
     3661 agcccgtc
//
LOCUS       DASTKC010000167         7184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1785800, whole genome shotgun sequence.
ACCESSION   DASTKC010000167 DASTKC010000000
VERSION     DASTKC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785800"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFR25_06630"
     CDS             <1..540
                     /locus_tag="VFR25_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9940759.1"
                     /translation="ELVHGRLPADDRRLALERFRSGEARILVTTTVVEVGLDIPDATL
                     VAIEHPERFGLAQLHQLRGRVGRAGRAGRCVLVTRSGLGEGARRRLHVFQTVSDGFRL
                     AEEDLKQRGPGEFLGTSQHGFPTFRAADPMQDQELIEAAQELSRELLDRAEAETSGGR
                     VEAWIETHVPGAERYLASG"
     gene            617..1771
                     /locus_tag="VFR25_06635"
     CDS             617..1771
                     /locus_tag="VFR25_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET9940760.1"
                     /translation="MTFECTSCQLRYKIDDSLIPPEGARVKCRRCSTLIDVPAPVIEL
                     APVDLELIPPPPPKRAQRIEEAPVERLAEPLPAPRVAEPLPAPRVADPLPAPRAADPE
                     LEAVDEGVTVEQQLAAEARSVPPSQDIEHNSSFVERFDAPSAASIPVDPAFEHIGAAA
                     AQASYTPAAPALETEPLGTPPPQPAFDSTPAFDSTPALAPVLETSAAPSLGEPSLAEP
                     SSAPPAGAPTIGEPSLGAPTLAPPSLGAPTLESNPPEPEAPAEAPALAAPTLATSDTA
                     PGEAPMATDNVVPLTLGTPIPDGLSPEEKTRHEKARRLARVLASDIAIYNREKKERGM
                     VDGNLVAVLGYEIKKSWETYKERVGADFANSTPYFRDALNDLLAEGKKIF"
     gene            complement(1780..3027)
                     /locus_tag="VFR25_06640"
     CDS             complement(1780..3027)
                     /locus_tag="VFR25_06640"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9940761.1"
                     /translation="MRGVSSLRLFRSGSFRALWIGQLVSIFGDRFTYLALLALVVERA
                     ADPANPARELALIPLFSFLPAILFSPVVGALVDGWNTRTTLLVSDALRGVVVLAMIPA
                     AETWGLPAAFALVFLLYMVNTFFLPARSAILPDLVPEDSLVEANSLATLAGVVATILG
                     SWLGGLWVERAGWRVGFALDGATYFVSVLALAFIRPRDLRPRAKRHASAAVAYRALAR
                     DVAEGARLSLASRPILGSMLAMALLWIAGGALHVALPTVIRSHGSGVVSGVGSVIAFA
                     ASGMVVGTLLLAAWGRGGSRAWVLAGSLVGAGIAVAAFARFPSPGAGAAAAFLAGLFV
                     ALLLIATESAIQQAARPEARGRVFALRDFLARLGVVVSAVVFGWLLERGGLTPGSAVV
                     LAGTILVLGGGVGALLGRARRGS"
     gene            complement(3027..4190)
                     /locus_tag="VFR25_06645"
     CDS             complement(3027..4190)
                     /locus_tag="VFR25_06645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940762.1"
                     /translation="MRLRIGIVSQAYLPTLGGVTEHVDATAKGLRRRGHDVTVVTSGI
                     PGRPRFEPGTVRVGHNIVLPYNGAQNDMTVGFDLPERLGALFDERRFDVIHVHCPVAP
                     VLPLLTLRLARVPVVGTFHSVSSDLPYRLFGGLLRPLYRRIDHRLAVSTVARDYIQRH
                     FPGAVEVLPNGVDLDRFRPRAPRGDDRVPTILFVGRLDPRKGLPDLIDACARLDVPFR
                     LVVVGDGPLRAPMERRAHRMLDGRVEFAGRVEQDELPDYYAASDVFCTPARSGESFGL
                     VLLEAMATGVPIVATSLPGYRTVLTPEREGILVEPRDSRAMALALRRLLLDPRLRARM
                     GAHGVETARRYGWDGIVERLEEVYASLLPVRLERRLAAVPAEEREPEFETAVR"
     gene            complement(4180..5043)
                     /locus_tag="VFR25_06650"
     CDS             complement(4180..5043)
                     /locus_tag="VFR25_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9940763.1"
                     /translation="MLQAIQTACATSSPRAHRIAARFIASLQYTLSRSRRSAVLANLT
                     QMAASGHPALTSAQARERTARRMFESLPLSWIEYFAEARADGGTRSPSFRGAELLYRA
                     LAQGRGAVIAAPHLGSWELAGLALARLGLEVHAVSGVQIHPLLARALRDRKAREGLVI
                     RTPSEGFAPLVAALRRGAVVVLLVDGDIHSRSLPAPFFGRRTPFPAGPAILARRARVP
                     ILHAHATRSEDGSHEVVFDGLDEADPSLALREDLARLTALVARAQERSIAENVTQWCI
                     FRPLWSTADAA"
     gene            complement(5245..5439)
                     /locus_tag="VFR25_06655"
     CDS             complement(5245..5439)
                     /locus_tag="VFR25_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940764.1"
                     /translation="MKRTTPSPAPWFLARRAREVYCRRDNRVYSSCPHSDTAALCPCL
                     HAVETADALFEPGDEEQPPT"
     gene            5546..5962
                     /locus_tag="VFR25_06660"
     CDS             5546..5962
                     /locus_tag="VFR25_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940765.1"
                     /translation="MASQKLVRCEIKRPENGSTRTVRYVPLEIYGLWEYLMRNRHQFE
                     ITDNVASLWIDVDENPEAAYGEGQYERVTEVTLLRYSEQDGMFGRVSRYFPTQDYPEL
                     KKLLLKHYESVREPGAPNLQLKERQGIWIKREPRLT"
     gene            6229..6804
                     /locus_tag="VFR25_06665"
                     /pseudo
     CDS             6229..6804
                     /locus_tag="VFR25_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
BASE COUNT         1240 a         2494 c         2499 g          951 t
ORIGIN      
        1 gagctggtcc acggccgcct gcccgccgac gaccggcgcc ttgcgctcga gcggttccgg
       61 agcggggagg cgaggatcct cgtcacgacc accgtggtgg aggtggggct cgacatcccc
      121 gacgcgacgc tcgtcgcgat cgagcacccc gagcgattcg ggctggcgca gctgcatcag
      181 ctccgcgggc gcgtcggccg cgcggggcgc gccgggcggt gcgtgctcgt cacgcggagc
      241 gggctcggcg agggagcgcg ccgcaggctt catgtgttcc aaacggtgtc ggatggattc
      301 cgcctcgcgg aggaggatct gaagcagcgc ggccctgggg aatttctcgg gacgtcgcag
      361 cacggatttc cgaccttccg cgcggccgat cccatgcagg atcaggagtt gatcgaggcg
      421 gcccaggagc tgagccggga gctcctggac cgggccgagg ccgagacgtc gggagggcgc
      481 gtggaggcat ggatcgagac gcacgtgccg ggggccgagc ggtacctggc gagcgggtag
      541 aatttcggcc aaagatgggt gcggcggcag ccgatacaag ggaccgagtg aacgtcacca
      601 gccgccggga gccgcgatga ctttcgagtg cacgagctgc cagcttcgct ataagatcga
      661 cgactcgctc attccgcccg agggcgcccg cgtgaagtgc cgccggtgct cgaccctgat
      721 cgacgttccg gctccggtga tcgagctggc gccggtcgac ctggagctga tcccgccccc
      781 accgccgaag cgcgcccagc gcatcgaaga ggcgccggtg gagcggctcg ccgagcctct
      841 gccggctccg cgtgtcgccg agcctctgcc ggctccgcgt gtcgccgatc ctctcccggc
      901 tccgcgtgcc gccgatccgg agctagaggc tgtcgacgaa ggcgtcacgg tggagcagca
      961 gctcgccgcc gaggcgcgga gcgttcctcc gtcgcaggac atcgagcaca attcttcctt
     1021 cgtggagcgg ttcgacgccc cctccgcggc gtcgatcccg gtcgaccccg cgttcgagca
     1081 catcggcgcc gcggccgcgc aggcctcgta cacgccggcc gcccccgcgc tcgagaccga
     1141 gccgctgggc actccgccgc ctcagcccgc gttcgattcg acgccggcgt tcgattcgac
     1201 cccggcgctc gcgccggtgc tcgagacgtc cgccgcgccg agcctcggcg agccgtcgct
     1261 cgctgagccc tcgagcgcgc cgcccgcggg tgccccgacg attggcgagc cgtccctggg
     1321 cgcaccgacg ctcgcaccgc cgtcgctggg cgctccgacg ctcgaatcga atccgcccga
     1381 gcccgaggct ccggccgagg ctccggcctt ggccgcgccg acgctcgcga cgtccgacac
     1441 cgctcccggg gaggccccca tggccaccga caacgtcgtt ccgctcacgc tggggactcc
     1501 gatccccgac ggactctcgc ccgaggagaa gacgcgccac gagaaggcgc ggcggctcgc
     1561 gcgcgtgctc gcgtccgaca tcgcgatcta caaccgcgag aagaaggagc ggggcatggt
     1621 ggacgggaat ctcgtcgcgg tgctcggcta cgagatcaag aaatcgtggg agacctacaa
     1681 ggagcgtgtg ggcgccgatt tcgcgaactc gaccccgtac ttccgcgacg cgctgaacga
     1741 cctcctcgcc gagggcaaga agatcttctg atacgggggt tagctccccc gccgcgcccg
     1801 tcccagaagc gcgcccacgc cgcctcccag gacgagaatg gtgcccgcga gcacgaccgc
     1861 cgatcccggc gtgaggccgc cccgctcgag gagccacccg aagaccaccg cgctcaccac
     1921 cacgccaagc cgcgccagga aatcgcgcag cgcgaacacc cgtccgcgcg cttcgggccg
     1981 ggccgcctgc tggatggccg actcggtggc gatgagcagc agcgcgacga agagccctgc
     2041 gaggaacgcc gcggccgcac ccgcgccggg cgatgggaat cgcgcgaagg cggccacggc
     2101 gatccccgcc ccgacgagcg atcccgcgag cacccacgcc ctcgatccgc cgcgccccca
     2161 cgcggcgagg aggagcgtcc ccaccaccat cccggaggcc gcaaaggcga tcacgcttcc
     2221 gacgcccgac acgacgccgg acccgtggct ccggatcacg gtcgggaggg cgacgtggag
     2281 cgcgcccccc gcgatccaga gcagcgccat cgccagcatc gatcccagga tcggcctcga
     2341 tgcgagcgag agccgggcgc cttccgcgac gtcccgggcg agtgcccggt acgcgaccgc
     2401 ggccgatgcg tgccgcttcg ctcgcggccg cagatcgcgc gggcggatga acgcgagcgc
     2461 gagaacggac acgaagtagg tcgcgccgtc gagcgcgaag ccgacgcgcc atccagcccg
     2521 ctcgacccag agcccgccca gccacgaccc gaggatcgtc gccacgacgc ccgccagcgt
     2581 ggcgagcgag ttcgcttcca cgagcgaatc ctcggggacg agatcgggga ggatcgcgga
     2641 acgcgcgggg aggaagaagg tgttgaccat gtagagcagg aagacgagtg cgaacgccgc
     2701 cggaagtccc cacgtctccg ccgcgggaat catggcgaga acgacgacgc cccgaagcgc
     2761 gtcggacacg aggagcgtcg tgcgcgtgtt ccatccgtcg acgagcgcgc ccacgacggg
     2821 cgagaagagg atggcgggga ggaagctgaa gagcgggatc agcgccagct cgcgcgcggg
     2881 attggcggga tccgccgcgc gctcgaccac gagcgcgagg agcgccaggt aggtgaagcg
     2941 gtccccgaag atcgagacga gctgcccgat ccagagggcg cggaagctgc cgctccggaa
     3001 gaggcggagc gacgagacgc cccgcatcag cggacggccg tctcgaactc cggctctcgc
     3061 tcctcggccg ggacggccgc gagcctgcgc tcgagccgga ccggaaggag cgaggcgtag
     3121 acttcctcga gacgctccac gatcccgtcc catccatacc gccgggccgt ctccacgccg
     3181 tgggcgccca tcctggcgcg gagcctggga tcgaggagga gccttcggag cgcgagcgcc
     3241 atggccctgg aatcgcgcgg ctcgacgaga atcccttccc gctccggcgt gagcaccgtg
     3301 cggtagcccg gcagggacgt cgcgacgatc gggactccgg tcgccatggc ttcgaggagc
     3361 acgagtccga agctctcgcc ggagcgcgct ggcgtgcaga acacatccga ggccgcgtag
     3421 tagtcgggga gctcgtcctg ctcgacacgc ccggcgaact cgacgcgtcc gtcgagcatg
     3481 cggtgcgccc tgcgctccat cggcgcacgg agtggcccgt cgcccacgac gacgaggcgg
     3541 aacgggacgt cgaggcgtgc gcaggcgtcg atcagatccg gaaggccctt cctcggatcg
     3601 agccgcccca cgaagagaat cgtcggcact cggtcgtcac ctcgaggcgc gcgcggccgg
     3661 aaccgatcga gatcgacgcc gttcggcagg acctcgacgg cgccggggaa atgccgctgg
     3721 atgtagtccc gcgccacggt cgatacggcg agccgatggt cgatccgccg atagagcggc
     3781 cggagaagac ccccgaagag gcggtaggga agatccgagg aaacggagtg gaacgtgccg
     3841 accacgggca cgcgcgcaag tcgcagcgtg agaagcggga gcaccggggc gacggggcag
     3901 tgcacgtgga tcacgtcgaa gcgccgctcg tcgaagagcg cgccgagccg ctccgggaga
     3961 tcgaacccga ccgtcatgtc gttctgggcg ccgttgtagg gaaggacgat gttgtgtccg
     4021 acgcgcacgg tcccgggctc gaagcgcggc cggccgggga ttccggacgt cacgaccgtg
     4081 acgtcgtgcc cgcgccgcct gaggcccttt gcggtcgcgt ccacatgctc cgtcacgcct
     4141 cccagggtcg ggagatacgc ctgggagacg atcccgatcc taagccgcat cggcggtgct
     4201 ccagagcgga cggaagatgc accactgcgt cacgttctcc gcgatggagc gctcctgcgc
     4261 ccgcgcgacg agcgcggtga ggcgcgcgag atcctcgcgc agcgcgaggc tggggtcggc
     4321 ctcgtcgagg ccgtcgaaca cgacttcatg actcccgtcc tcgcttcgag tcgcgtgcgc
     4381 gtggaggatc gggacccgcg cccggcgcgc gaggatggcc gggccggcgg gaaagggagt
     4441 tcgccgtccg aagaagggag ccggcagcga acgcgagtgg atgtccccat ccacgagcag
     4501 caccacgacg gctccgcgcc tgagtgctgc gacgagcggc gcgaagccct cgctcggggt
     4561 ccgaatgacg agcccctcgc gcgccttccg gtcgcggagc gcgcgcgcga ggaggggatg
     4621 gatctgcacc ccgctcaccg catgcacctc gagcccgagc ctcgccaagg cgagcccggc
     4681 cagctcccag ctccccaggt gcggcgccgc gatcacggcc ccgcggccct gggcgagcgc
     4741 gcgatagagg agctcggctc cccggaagct cggggagcgg gtcccgccat ccgcacgcgc
     4801 ctccgcgaaa tactcgatcc aggacaaggg cagactctcg aacatgcggc gcgccgtgcg
     4861 ctcgcgagcc tgcgcgctcg tgagcgcggg atgtccggag gcggccatct gggtgagatt
     4921 cgcgagcacg gcgctcctcc gtgagcggct cagcgtgtac tggagggagg cgatgaagcg
     4981 tgcagcgatc cggtgggcac ggggagagct ggtcgcgcac gcggtctgga tggcttgcag
     5041 cactaggctc ggcaacacgt ccggatcgcg cgcgatggtt tgcctctctc ccccttggtt
     5101 cgaggacgct acaccagcgt ccaaaaaggt gtcaaggcga gcggggcaaa gccgtgcggt
     5161 ccgaacccgt tgacttgacg agaggtcggc ccctattgtg gcggcggcca aatcgagcca
     5221 gggatcggct cgaatcagcc gagatcacgt cggcggctgc tcctcgtctc caggctcgaa
     5281 gagggcgtcc gccgtctcca ccgcgtgcag gcacggacaa agggcggccg tgtcgctgtg
     5341 cgggcaggac gaatagacgc gattgtcgcg ccggcaatag acttcgcgtg cgcgacgcgc
     5401 gaggaaccat ggtgcgggcg aaggtgtcgt tcgtttcatg gcgctcctcc tcggtcgaac
     5461 gtccgccgag cggcggactc ggtacgccgc aaccgcttcc aatgcggcaa ggctgcatga
     5521 ggcatgccat ggaatggggg gactgatggc cagccaaaag ctggtgcgct gcgagatcaa
     5581 gcgtcctgaa aacggatcca ctcgcaccgt ccgctacgtg cctctggaga tctacggact
     5641 ctgggaatac ctcatgcgga accggcacca gttcgagatc accgacaacg tggcgtcgct
     5701 ctggatcgac gtggatgaga atccggaggc tgcctacggc gagggacagt acgagcgcgt
     5761 gaccgaggtc acgctgctgc gctactcgga gcaggacggc atgttcggac gggtgagccg
     5821 ttatttcccg acccaggact atccggagct caaaaaactg ctgctcaagc actacgaaag
     5881 cgtccgcgag ccgggggctc ccaacctcca gctcaaggag cggcagggga tctggatcaa
     5941 gcgggagccc cgcctcacgt aaccgcaatc tgcgcttcat catgcggacc gcacgccccg
     6001 tcgcgcaccc tggcttcggg gcactcgccc cgcgcttgtg gcccgtctcc gcatccgacg
     6061 attcagccaa agtttagata cgagcccgcc gatctctcag gcgtagctgt cgagagtgct
     6121 gtggcacata acgtgctgct tggcgcggcg ggccggtcga actccaatcc cacgcatccg
     6181 cgcccaacgc agagcgacgt caatgaaact catcgaggga gcgacgccat ggtcgtggtg
     6241 agctattccg ggcgggagat caacgcgaag atcgtctact acggtcccgg tctgagcgga
     6301 aagacgacca acctcgagaa gatctacgac agcgtccccg agaccaatcg cggccgcatg
     6361 gtctcgatga agacgcagac cgaccgcacc ctgttcttcg atctccttcc cctggatctg
     6421 ggcgagcttc aggggatgaa gacgcgcttc ctgctctaca ccgtgccggg acaggtctac
     6481 tacaacgcga cgcgaaagct cgtgctgaag ggcgtcgacg cgatcgtctt cgtcgccgac
     6541 tcggcccccg acaagatggc cgagaatcgg gagagcctct ccaacctgga gcagaacctc
     6601 aaggcctacg gactcgacat ccagacgatt ccctgggtga tccagttcaa caagcgcgat
     6661 ctcccgggcg cgctctccat ccagcagatg aatcaggagc tgaaccgcca caacgtgccc
     6721 acgttcgagg cgcaggccgc gaactcggtc ggcgtgttcg agacgctgcg cggggtgtcg
     6781 aagctccttc tcgccaagat ctccaaggac gtcatcgatc gtgatcgggg aagcctgacc
     6841 gcgccgcctc gggtcgcatc cgaggagccg aaacggcctg cggccgtcga ggagaccccg
     6901 acccctacac ccgaagcgcc caagtcggtc ggcgggaaga tcctccagtc cctcaagcgg
     6961 ggcgggaagg ctcagcccga gccgcacacg ccgacgaccg cttcgaatcc gccgctcgag
     7021 ccggcgggac cgttctcgca gtccatcgag ccccagactc cgttcacgcc gccgtcgagc
     7081 atgcagcccg acgagaccgt gctcacgagc gacgatctga tcccgctcgg gggcttgcag
     7141 gccgcgaaga agaagggcga cgacgaaggc ttctccgcgg gcga
//
LOCUS       DASTKC010000168         5837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1788706, whole genome shotgun sequence.
ACCESSION   DASTKC010000168 DASTKC010000000
VERSION     DASTKC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788706"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFR25_06670"
     CDS             <1..302
                     /locus_tag="VFR25_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9940766.1"
                     /translation="ARPRVKVLPMFRVGREAERSRGYDERERLTPEHLLGFDMQKLQC
                     STSRMVTSRGVYVCPILIDSEDARLGPTLRESLRSFPLAHGACHTCWATGATCRN"
     gene            290..1078
                     /locus_tag="VFR25_06675"
     CDS             290..1078
                     /locus_tag="VFR25_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9940767.1"
                     /translation="MPELMEPIAVFGGPYGNHLALRAVLEDAARRGAAAVYCLGDLGG
                     FGPRPGAMVSLLEEFQVVTVAGNYDRALAERQPDCGCGYTHPKDNDYARIAYAYTDAR
                     ASDRDRGWMGRLPESLRLGRGGRRYLLCHGSPRQANEFLWESASSDAFLERLARDARA
                     DQILCTHTGIHWQRELPQGARIVNVGAIGRPANDGLRVAWYAWFAADRHDAQFVPVEY
                     DVEAVAAELDAEGLPPEFGETIRTGWWTTCLEILPAKERARGRW"
     gene            1079..2362
                     /locus_tag="VFR25_06680"
     CDS             1079..2362
                     /locus_tag="VFR25_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9940768.1"
                     /translation="MADPVGLLREYAAQVRGASRNPRLYLAGMFLFGVGQSIFGLLFN
                     LYLRDLGFTDATIGQILSKVALGAAVAALPAAFLFRSMPARTLLVGGAALASLGYVFQ
                     ASFVQPELLLLVAFLTGMVLTIFRLSIAPVVMKESASERRPFLFSASFGVFFLSAILG
                     SAVGGALPHLVHVVAPASSQALRLSLFAAAAVTLASTFPFLAMRSGEESQGAGSKGGP
                     GALEQIQDLLEIDWGLHLKLILPSAMIGLGAGLIIPFLNLYFKDRFGLAEGEIGILFS
                     VMQGFMVAGNLFGPAVSRRLGLVLGVVATQLLSVPFMIVLAVSHLFPLVALCFFLRGG
                     LMNMNQPLVSHFAMEVVPERDHAITNSLLSLSWYLAWTVSADIGGTLIERKGYEEPLL
                     LAAALYVGASVLYWIFFKDVAEARLPRTEVEIPEA"
     gene            2547..3131
                     /locus_tag="VFR25_06685"
     CDS             2547..3131
                     /locus_tag="VFR25_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9940769.1"
                     /translation="MKRTMALVFAALFCVAGFAAHANANVVGSMELNLGYVKSSNEVT
                     PVGDSMGGGLGFGAAYWRSASPSISWGAEVSYDNLGTANYDNGSTTDNEISSKVFRVE
                     PAMRFNFGAPTGPSFFAQGGAGYYSVSSKIDDSVFGAADDSQGKFGYMLGAGVGFPVG
                     PKTKMNLQGQYHSVSTEGESLNYLAFRAGVGFSI"
     gene            3373..3933
                     /locus_tag="VFR25_06690"
     CDS             3373..3933
                     /locus_tag="VFR25_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940770.1"
                     /translation="MNGRIAVRLLLAAVLTLAVPTLASAQTLSGIQSASLSASLAQSL
                     SISITSGSSVNFTLAANAAANGDVPVVVSSTWNLNPGQTGAVSLYGYFDVPASALTDG
                     SNNIPTSWVEGRVTTGTPTSYTAFTQTNPVGPAGGSLALFSEAITGANKIKTRSDNLD
                     LRINLTGQTLPASSYTGTLRLQARAI"
     gene            4032..4754
                     /locus_tag="VFR25_06695"
     CDS             4032..4754
                     /locus_tag="VFR25_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940771.1"
                     /translation="MGAFALAVLALLGLGVGPRSASAQTVRPVVVEHHGKKAQARFEL
                     VNDGLVPLNVVLEPKSFDVSIDGEVTYRPLDSSVHLKLSAMSLRIPAKQSRWVFYEAT
                     ADSYPAWFVIPCTFSGMPKHQGLNVTVELPHTVYLVQNDAVRREDVKIHSAVYAASSA
                     KIYLEVENMSPRLGRVSTAEVRGKDGRSAFPSFPILPNRIRRVVIPWEGAGVPTTLRL
                     ALQGFTIETPLGNASQVAGDEK"
     gene            4744..>5837
                     /locus_tag="VFR25_06700"
     CDS             4744..>5837
                     /locus_tag="VFR25_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940772.1"
                     /translation="MKNERPHHAAIAGVALLALLAASAAHAQVFEVQAGASSLYDASG
                     GSVRMHAEGAEAWLGLAGVDPLRVGFGASTRYHGAGLSVGDAIVPFHLPTDALHGGQS
                     LVARGVGLGYEDRDVRVAAFGGATANATSYPYTFWAEGDRPVGLVFLESKVSNSVRLS
                     SRSLYSDGMTTILALDYKPGLGYGGAVAGGTGADEGYAAASAWYERGLLTARAGYVVA
                     GDGFRRVITPQPGAAELIGPSLELGVRVRPELTLGASHTESVQPDPGPRADRKGTVNQ
                     WLATGRVLGTSLGTAYFRSRSGEAGTDGVSLSFDRAVPSVGWLRVNGSWLYSDPLTGA
                     PFRMLVGGFRETVSPRLELIQLATHSGDNTT"
BASE COUNT          834 a         2039 c         1952 g         1012 t
ORIGIN      
        1 tcgcccgccc gcgcgtgaag gtgctcccga tgttccgggt cggccgcgaa gcggagcgga
       61 gtcgcggcta tgacgagcgg gagcggctga cgcccgagca tctcctcgga ttcgacatgc
      121 aaaagctcca gtgctcgacc agccgcatgg tgacgagccg cggcgtctac gtctgcccga
      181 tcctcatcga cagcgaggac gcgcggctcg gaccaaccct ccgcgagtcc ctgcgctcct
      241 tcccactggc gcacggcgcc tgccacacct gctgggcgac cggggcgacg tgccggaact
      301 gatggagccg atcgccgtct tcggcggccc ctacggcaat cacctggcgc tccgcgccgt
      361 gctcgaggac gcggcgcggc gcggcgccgc cgcggtctac tgcctcggcg acctgggcgg
      421 cttcggtcca cgccccggcg cgatggtctc gctcctcgag gagttccagg tggtcacggt
      481 cgcggggaac tacgaccggg cgctcgcgga gaggcagccc gactgcggct gcggctacac
      541 gcatcccaag gacaacgact acgcgcggat cgcctatgcg tacaccgacg cgcgggcgtc
      601 cgatcgggac cgcgggtgga tgggtcgctt gccggagtcc ctgcgcttag ggcgcggtgg
      661 ccggcgctat ctcctctgcc acgggtctcc ccggcaggcg aacgagttcc tctgggagtc
      721 cgcctcgtcc gacgcgttcc tcgagcggct cgcacgggac gccagggcgg atcaaatcct
      781 ctgcacccac acgggaatcc actggcagcg agagctgccc caaggcgcgc gaatcgtcaa
      841 cgtgggagcg atcgggcggc cggccaacga cggtcttcgc gtcgcgtggt acgcatggtt
      901 cgccgccgac cgccacgacg cgcagttcgt gccggtcgag tacgacgtcg aagccgtggc
      961 cgcggagctg gacgcggaag gactgcctcc cgagttcggc gagacgatcc gcacgggatg
     1021 gtggaccacc tgcctcgaga tccttcctgc gaaggagcgt gcccggggcc gctggtagat
     1081 ggccgatccg gtcgggctcc tgcgcgagta cgccgcgcag gtgcgtggag cgagccggaa
     1141 tccacgcctc tacctcgcgg ggatgttcct cttcggggtg gggcagtcga tcttcgggct
     1201 tctcttcaat ctctacctcc gcgatctcgg cttcaccgat gccacgatcg ggcagatcct
     1261 ctccaaggtg gcgctgggcg ccgccgtcgc ggcgctgccg gccgcgttcc tgttccgcag
     1321 catgccggcg cgcacgctgc tcgtcggcgg cgccgctctc gcctcgctcg ggtacgtctt
     1381 ccaggcgtcg ttcgtgcagc ccgagctcct cctcctcgtc gcgttcctga cgggaatggt
     1441 gctcacgatc ttccggctct ccatcgcgcc ggtcgtgatg aaggagagcg cgtccgagcg
     1501 gcgcccgttc ctcttcagcg cttcgttcgg cgtcttcttc ctctccgcca tcctcggatc
     1561 ggcggtcggc ggagcgcttc cgcacctggt tcacgtcgtc gcgcccgcca gcagccaggc
     1621 gctgcgcctg agcctcttcg cggcggcggc ggtcacgctc gcgtccacgt ttcccttcct
     1681 ggccatgcgg tcgggcgagg aaagccaggg cgccgggagc aagggcggcc ccggcgcgct
     1741 cgagcagatc caggatctgc tcgagatcga ctggggcctc catctgaagc tcatcctccc
     1801 gtcggcgatg atcgggctcg gcgccgggct catcattccg ttcctcaacc tctacttcaa
     1861 agaccgcttc ggcctcgcgg agggagagat cgggatcctg ttcagcgtga tgcaggggtt
     1921 catggtcgcg ggcaacctgt tcggtccggc ggtctcccgg cggctcgggc tcgtgctcgg
     1981 cgtggtcgcg acccagctcc tctccgtgcc gttcatgatc gtgctcgcgg tctcgcatct
     2041 gttcccgctg gtcgccctct gcttcttcct ccgcggcggg ctcatgaaca tgaaccagcc
     2101 cctggtctcc cacttcgcga tggaggtggt cccggagcgg gaccacgcga tcacgaacag
     2161 cctcctctcc ctctcctggt acctggcctg gaccgtgagc gccgacatcg gcgggacgct
     2221 catcgagcga aaagggtacg aggagcctct tctcctggcc gcggccctct acgtaggggc
     2281 ctcggtcctc tactggatct tcttcaagga tgtggctgaa gcgcgccttc cacggaccga
     2341 agtcgagatc cccgaagcct aactgttgct gcgtaagggc ttgtagttcc gggctccctg
     2401 caatctcggg tcaagtttcc ccccctggat gccgatgagt gtccggagca ggagggggag
     2461 ccgagcctgc acggacgcgg gatgcacgcg gctccagggt ggctgggcgg atgaccacgg
     2521 agttctaaaa ccggaggggg aagcaaatga agcgcactat ggccttggtt tttgccgctc
     2581 tgttctgcgt cgccggcttc gcggcccacg cgaacgcgaa cgtggtcggc tccatggagc
     2641 tcaacttggg ctacgtgaag tcctcgaacg aggtcacgcc ggtcggcgat tcgatgggcg
     2701 gcggtctcgg tttcggtgcg gcctactggc gctcggcgtc cccgtcgatt tcgtggggtg
     2761 ctgaggtgtc ctatgacaac ctgggcacgg ccaactacga caacggcagc accacggaca
     2821 atgagatctc gagcaaggtg ttccgcgttg agccggcgat gcgcttcaac ttcggcgcgc
     2881 cgacgggccc gagcttcttc gctcagggcg gcgctggcta ctacagcgtg tcctcgaaga
     2941 tcgacgacag cgtctttggc gctgcggatg acagccaggg caagttcggc tacatgctgg
     3001 gcgctggcgt tggcttcccg gtcggtccga agacgaagat gaaccttcag ggtcagtacc
     3061 actcggtttc gaccgagggc gagagcctca actatctcgc gttccgcgct ggcgtcggct
     3121 tcagcatcta agcctcgcgg cacggctgta gcgatcgggg tggcgtcgca agacgccgcc
     3181 ccgtttcgtt ttttccctgc ctgtccgttc gggcccaaaa acttaggggc cgggcgtaat
     3241 cgcaccgggg gggtgtcgct acaaaggtct acacattcct gtaacaaccg ccgatgaatc
     3301 tcggagcgcg aagcgcgggc gtttgtctcg gccgcccgtc tcaggcgccc acacatcacc
     3361 acgggggtcc tcatgaacgg tcgtatcgct gttcgtctcc tcctggccgc ggtgctcacg
     3421 ctcgcggtcc cgactctggc ctcggcgcag acgctctcgg gcatccagag cgcgagcctt
     3481 tcggcgagcc tggcccagtc gctcagcatc tcgatcacga gcgggtcctc ggtcaacttc
     3541 acgctcgccg cgaacgcggc cgccaacggc gacgtgccag tggtggtctc gagcacgtgg
     3601 aacctgaatc ctggccagac gggcgcggtt tcgctctatg gctacttcga cgttccggcg
     3661 tccgccctca cggacggctc gaacaatatc ccgacctcgt gggtcgaggg ccgggtgacg
     3721 acgggcacgc cgacgagcta caccgccttc acgcagacga acccggtcgg cccggccggc
     3781 ggcagcctcg cgctcttcag cgaggccatc acgggcgcca acaagatcaa gacgcgcagc
     3841 gacaatctcg acctgcgcat caacctgacc ggtcagacgc ttccggccag cagctacacg
     3901 gggaccctga ggcttcaggc tcgggccatc tgaggggttc gatgtcgcga gttccacgga
     3961 aggattccga ggcgcggggg ttctgccccc gcgccttcac tcgcttttca agccgtcacc
     4021 gcggacgcgg tgtgggcgca ttcgcgctcg ccgtcctggc gctcctcggc ctcggtgtgg
     4081 gcccgcgcag cgcttctgcc cagaccgtgc gccccgtcgt cgtcgagcac cacggcaaga
     4141 aggcgcaggc caggttcgag ctcgtgaacg acgggctcgt tccgctcaac gtcgtcctcg
     4201 agccgaagag cttcgacgta tcgatcgacg gcgaggtgac ctaccgcccg ctcgattcgt
     4261 cggtgcactt gaagctctcc gccatgagcc tgcgcattcc ggccaagcag tcgcgctggg
     4321 tgttctacga ggcgaccgcc gattcgtatc ccgcctggtt cgtgatcccg tgcacgttct
     4381 cgggaatgcc gaagcatcag gggctcaacg tcacggtgga gctgccgcac acggtctacc
     4441 tggtccagaa cgacgccgtc cgccgcgagg acgtgaagat ccactcggcg gtctacgcgg
     4501 cctcgagcgc gaagatctat ctcgaggtcg agaacatgtc cccgcggctc ggccgcgtct
     4561 cgacggccga agtgcgcggc aaggacgggc gctccgcctt cccgagcttc ccgatcctcc
     4621 cgaatcgcat ccgccgcgtg gtcatccctt gggagggggc cggcgtgccc accacgctcc
     4681 gcctggcgct ccagggcttc accatcgaga cgcctctcgg gaacgcctcg caggtggcgg
     4741 gcgatgaaaa atgaacgccc tcatcacgcc gcgatcgccg gcgtcgccct cctcgccctt
     4801 ctagccgcct ccgccgccca cgcccaggtc ttcgaagtcc aggccggcgc gtcctcgctc
     4861 tacgacgcga gtggcggctc cgtccggatg cacgcggagg gtgccgaggc gtggctcggt
     4921 ctcgccggcg tcgatccgct tcgcgtggga ttcggcgcga gcacgcgcta ccacggcgcc
     4981 ggcctctccg tcggagacgc gatcgtgccg ttccatctac ccaccgacgc gctccacgga
     5041 ggtcagtccc tggtcgcgcg cggcgtcggc ctggggtacg aggaccggga cgtgcgcgtg
     5101 gccgcattcg gcggcgccac ggccaacgcc acgagctatc cctacacgtt ctgggccgag
     5161 ggggaccgcc cggtggggct cgtcttcctc gaatcgaagg tctcgaacag cgtccgactc
     5221 tcgtcccgga gcctctactc cgatggcatg accacgatcc tggcgctcga ttacaagccg
     5281 gggctcggct acggcggagc cgtcgcgggc gggacgggtg cggacgaggg ctatgcggcc
     5341 gcgagcgcgt ggtacgagcg gggactcctc accgcgcgcg ccgggtacgt cgtcgccggc
     5401 gacggattcc ggcgcgtcat cacgccgcag cccggcgcgg ccgagctgat cgggccgagc
     5461 ctcgagctcg gggtacgcgt ccggccggag ctcacgctcg gtgcctcgca caccgagagt
     5521 gtgcagcccg atcccgggcc gcgcgccgac cggaaaggaa cggtgaacca gtggctcgcg
     5581 accggccggg ttctcgggac gagcctcggc accgcctact tccgctcgcg atccggcgag
     5641 gcgggaacgg acggtgtctc gctctcgttc gatcgcgccg tgccctcggt cgggtggctg
     5701 cgggtaaacg ggagctggct ctactcggat ccgctcacgg gcgccccgtt ccggatgctg
     5761 gtcggcggat tccgcgagac ggtctcgccg cggctcgagc tgatccagct cgcgacgcat
     5821 tcgggggaca acacgac
//
LOCUS       DASTKC010000169        76230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_844486, whole genome shotgun sequence.
ACCESSION   DASTKC010000169 DASTKC010000000
VERSION     DASTKC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 76230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76230
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844486"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..617
                     /locus_tag="VFR25_06705"
     CDS             357..617
                     /locus_tag="VFR25_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940773.1"
                     /translation="MSPNDQDKEIVERLIAETNLSARMDALIGEICDKLREYGVASPH
                     KAFNDLVDPFREYFMLEYEEERGMEEAEEEEADDEEEDERSY"
     gene            complement(663..1550)
                     /locus_tag="VFR25_06710"
     CDS             complement(663..1550)
                     /locus_tag="VFR25_06710"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET9940774.1"
                     /translation="MRAAAPAPTEGPLLGAHMSTAGGLANAVARALSVRSRVVQLFTR
                     NCNQWRAKPLNDPEVGAFREAWEQSGLVSVAAHDSYLINLASPDAVLRERSIGALVEE
                     LRRCEALGIPYLVAHPGAHVGEGVRDGCDRVAESLNRALAEAHAPDVMILLETVAGQG
                     TTLGRRFEELHRIRDGVKLTGRVGVCLDTCHVHAAGYDLVTERGWEETFMEFDAILGL
                     ESLRWMHLNDSKNERGSRVDRHEHIGRGRLGVEPFARILKDPRLHAVPKTLETPKGRD
                     GVVMDRRNLALLRKLGESG"
     gene            1810..2334
                     /locus_tag="VFR25_06715"
     CDS             1810..2334
                     /locus_tag="VFR25_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9940775.1"
                     /translation="MRATRPVPRYCVLCGTALRTIREHGVARKACPKCGFIAYRNPVP
                     ACGVVVMRRGQVLLARRAHQPRIGAWGIPAGFMEYGEHPEVTAVREAREETGLRVKLD
                     GLFGVYAGRDDPRTRAVLILYRARITGGKLTAGDDASEVDFFPLDRLPRPIAFRAHTE
                     ALRDLKRWLARERR"
     gene            2331..3476
                     /locus_tag="VFR25_06720"
     CDS             2331..3476
                     /locus_tag="VFR25_06720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HET9940776.1"
                     /translation="MSVRVLHLADIHLGAAFPSMRERGPERTRDLLAAFLRSIDFATR
                     DPKPVDMVVIAGDLFDTHDPEEGLVYQVEAAIDRLAQAGLPVLISPGTHDAPSYRRCV
                     YRRLRLPSSAHIFLSPTLEPGPLFTVGSETVQTYGIAYDAALCERPFADFRPSGLVDY
                     HLAVVHGALQDNPAWKIRPTDLPMTRPEIAKSGLHYVALGHFHNFAEIRENGVVAVYP
                     GTLEGKRFGENGPRYLVVATLSRDGVTLDRTPWNVRTLHDAVIDLGLSELRGEDQLES
                     RLLAFAGDREITRIRIEGPADFVFDADRVQRRLAPKFFHLEIEDKTYVVNAALLTRYK
                     DEPTVRGVFVRRMLEKIDRAPDIATREAATHALRLGLAEFQNPRHAP"
     gene            3466..5688
                     /locus_tag="VFR25_06725"
     CDS             3466..5688
                     /locus_tag="VFR25_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9940777.1"
                     /translation="MRLERVTVEGFGPLTNFDAVLEPKRLNLFIGPNESGKSTFASAI
                     VSAIFGCVSVEQETLTRPWSGGKHAVAVTFTTLTGRFRVRRDFATHDVRVDRMAGDQD
                     TVESTVFQGIANPRGKSAEQLQYEELLRGWFGFTEARLFRESSFVHENGLETQVSPEL
                     RHIVSGAVEADYQEIENALMERLDSLTREHPFDPRARKRVNRSIENREERLEQLRERR
                     KRSESVLTELKSRVAEQDGMESRLSDLKSDLATKERLVNDLDTWLRLRDDQRRHLKRA
                     PDIGTELVNVRRARGQVQDIDRKIREDLGYLANAPEEAEADLMRLGMLRSQRAKHQKT
                     AEEARKKTDGSGSGSPMVAVLISILLGGGGAAGAWLTLHNPWVAGGIAGAGLIGAVMF
                     GAMLGQKSQRSRSLAETHVRVLEENIRTLSQEIDQVEIRVNPYLAGRTMEVVLEDVKR
                     YRAMMQERRESAAVLQSMPVPERLEAESKEIDEAVSTLRAKEKALLQQSPFLAPLRED
                     AVKAAEAAERLRREATALRTKIQAEQDSFDAALRRGGGGPSENDAENLAILDETIASE
                     EEALAREERQRDALLIALEVLRDSVLAYQEEHVHRLAREAGDTLERLTEGRYVKVKLD
                     ADLKPTLATPEREGLPLESLSRGTRDAFYLALRAALAQELSAREPLPLILDDPIAHFD
                     EERRGSLIRILEELAGNIQVLLLTHDRGVLNQVREAHVTGVGAVARPRDAKTKVSVRP
                     "
     gene            5789..7723
                     /locus_tag="VFR25_06730"
     CDS             5789..7723
                     /locus_tag="VFR25_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9940778.1"
                     /translation="MTTPTTPSSPSRPSPAPSPAGKPGSPEQRADRIRDQVLALDVKR
                     VADWIGALVRTIKATRTYLSNNPTLHKFQVELESRTWSCLKEAGDITLVVQQFDLLYE
                     NYSVYHNADRNDSLAFRFYGDGVRVITFREGLEPHELRAFIDVLKRSMETAQVQDDVV
                     TLLWERDFRHIEYQFIPLEDLTEESGEILNPEDEELAVSEGGGIPWPSGFAPSSEDEM
                     PAQVVDVAPEQSTDAPTGEASTVTGPTRERSDDWEPTVHAAPAYDSTIGVQFGLTDEE
                     NATLEVSIREEEGRQLGREVLEIVATILAHEDTPAGFLDSATAFQRFIELAIEEGEVA
                     RANDLVARLRSIAADCAKKNAEFPGLAEQVVREVGRPSFLGLMAPTLNARPEIDPGIL
                     TNFLVQLGPSAAPAICDLLGQVNQMKFRRALCEALAISCKNDVEVLIERLGDTRWYVI
                     RNIVYVLGRIAHQGVERALDRALHHEDVRVRKEAVRALGNIESPTSRAYLVSAFRDTD
                     AGVRIQAAMTLAMRRDDRASQSLLGVIQGTEFGRRDTNERQAFFEALGRCGSDALVPR
                     LDQMLTKGGLFRSSNEEERLHAALALAWLGTPNALQVLNREITSKREPIRKAVETALS
                     AVRNAGLATKDPAPGDEEDE"
     gene            7720..9195
                     /locus_tag="VFR25_06735"
     CDS             7720..9195
                     /locus_tag="VFR25_06735"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET9940779.1"
                     /translation="MSGATRTTLPTTRSTRDDTSSRLVLQGRSLSIQLCVLLKTARIH
                     DVGNLAFQGPMSAIIETVDQMWTAEGKFKLQAIGDFLYVNQLRLRVDASSYPSYQYLI
                     DELKTRDLSGFQFAGRLTPPEVKKFVRLFLDVDPKSESPFDDFTDSLAKLSVQNIVPI
                     RTVMPKEGAVTAEERRDGRKAAKRGFYRAIESARTVMLSARDHRPVDLRKAKRAVQSI
                     VDLIIDEEFSLLGLTAIKNHDEYTFQHCVNVSILSIAVGQRLGLSKKMLGELGVAALL
                     HDLGKASIPPWVLNKAGKLTPEEWKLVVDHPVQGVKMIARMRGLNDLALRSMIVAYEH
                     HINVDKSGYPQLEGHMDQSLFSRIVAIVDCFDAMTAHRAYRKSAFPPYAALHQIISQN
                     RNKFDPLLIKAFINTVGMYPAGTTVLLDTNEIAVVISHNATDIFRPRVQVVADSDRKP
                     VQDGTIIDLTARDEANATYLVTIVSALDPEEYGINIADVLT"
     gene            complement(9206..11206)
                     /locus_tag="VFR25_06740"
     CDS             complement(9206..11206)
                     /locus_tag="VFR25_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9940780.1"
                     /translation="MRPILLFPGLAILLFAPFLIPGSAASPLDIPPPPPDPHLIATIL
                     GPSIGFGSFIAGVGDVDGDGYEDLLAAVPGWFGGSAFLYLGSAQVDTTLDLLLAKPSN
                     PLTYGYGSVIAAGDLNGDGYSDIVLGDPLAIPGHDAEQACYVYLGSKVPDAQADLVLH
                     DPEPRQAPTRQFFGSALSVMDLDRDGFADLAIGKRADPQSFGRVYIHSGSASLDAGPD
                     ASFSPPLPPIVGWGFPTSMTPAGDMNGDGYSDVLVGESGFGSPPCPLGHGRATLFLGG
                     AVTDTVPDAFLAMTPLSIPSLTQPMSSAGDFNADGFDDLVVGAPVVHDDGSISSGRAY
                     VYFGSASPVSYPSPDLVLASESDSTFGRWVFGGKDVSGDGVPDILVATTFEIVVYFGG
                     HSPDAVPDVTLRYDGTSIPSGTILTAADWDGDGVAEIFVGSPNQRYGRIYVFATSTSL
                     DATAFLRGGRHPIPLARSGDLTLQVQPVGASYENEDVDPSTLTLSYAGPGSTTHIAAV
                     QGKTMPEGDTNHDGIRELAASFRSEDFRTLFEPFRGRMDVEVAIDGRLFDHRRIHASA
                     MLTVLGTGEIRPARISPNPLNPEGMLEFTLPSPGAVTLRLYDVSGRCVRTLIHGATYG
                     AGAHEVPFAARNERGEALPSGVFFYSLELSSGVQRGRFVVAK"
     gene            11391..12785
                     /locus_tag="VFR25_06745"
     CDS             11391..12785
                     /locus_tag="VFR25_06745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9940781.1"
                     /translation="MSLRPPRALIVDKDSSETAELKAFLSANDIDVEWAKDGEQAFNI
                     LDGASANGSGETAPPGPEVVITELKAHRVDGMRLLEVAKKRNPEVCVIFIADQGSLEL
                     ATEAMRQGAYDFQHRPLNRDKMLAVIRRALSHQQLVSRAEDLADRLDRRLRIPSLTGQ
                     SRVMEDLKDKILQIAPTRATVLIYGETGTGKELIAQAIHQLSPRKDERFVKLHCAELS
                     ANIIESELFGHEKGSFTGADQQRKGRFEIADHGTLFIDEISEISAPIQTKLLRVLQDR
                     QFERVGGNETVTVDVRVIAATNRRLDLMAARGLFREDLFYRLKVIQLEVPPLRDRRED
                     IPLLIEAFIREFDKEHGRKVTGVTRGVVDRMMEYEWPGIVRELKNTIEEMVIFAQGRK
                     LLDVSDLPITIRQQRASAATAMPVSVGMSMNEIERAALEATLRSVGYDKQRAAKILGI
                     GLRTLYRKQKEYGL"
     gene            12782..13246
                     /locus_tag="VFR25_06750"
     CDS             12782..13246
                     /locus_tag="VFR25_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9940782.1"
                     /translation="MSERTETAAPALGGRPAQGISEVILVVKDVARSFAFYRDVVGLA
                     VDNAGNEKFAWLWAGPPGKSQRIGITTGPLSFGAAHVKGPLHFAFGTEDERIEELKAA
                     LERHGLEVEGPVSFPFWGARSIYFSDPDGNRVEFCGFGPTQGTGRVHIKAKR"
     gene            complement(13280..13954)
                     /locus_tag="VFR25_06755"
     CDS             complement(13280..13954)
                     /locus_tag="VFR25_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940783.1"
                     /translation="MSANVALIRAGLRTLRGLTLAAGVASAAGCGAVLDLAEGDAPPR
                     KSFSWHAGPRAHCGTFGVTELTVLRRLNEDERHASEERYYLSFDGGAAHNVTSRISIG
                     GSLFAGGDGSDRTHFGLRGRLRYWLGKSASVDLAPGLILFGHEEDNKELVTPAPILRV
                     GVNPSASFGIAYQIFETKRRDTHGEVKERGSYVGAQLGSAAGLAGTGVVLAASVIFIA
                     LVLSSL"
     gene            complement(13995..16229)
                     /locus_tag="VFR25_06760"
     CDS             complement(13995..16229)
                     /locus_tag="VFR25_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940784.1"
                     /translation="MDRLRKADRLALLLVALSIAIRLWGVADRLPDPTLKINVLDDSV
                     VEETDRTTMGRAWVMWSGGLRHWDLNPHTGGWPAFSFYFALVCQIAFKAWWGLSHPDG
                     SARDFVEFFTEHVDQCFLLARLAGVLVGAVTVFLTFRLGQRLAGREVGLLGGLLLALN
                     PLHIQTSQHVADPNLLALLFVLLAAIAMTRLAETGSERDAIIAGAMIGFAGACKYVPL
                     ILVIPLAVAYGRDFWRSRGYGLALLAVFAALVVATPFTFIDWKTTLRDIGTQRSALFS
                     DWVGQTTFPFSLPTYITVSLPHAMGWAAYLLGIAGSGYLWRRGRAAQAIALIPAVMVL
                     ANGVLKAAQERYMLVAMPVLFIAAALAVIEAGRWLMAREAGRRAVVLAPALLALTAVA
                     WPLPEYVGLRRALALPDTRHVVRRWILENIPATAPMGVELYGPVFRADERSIVIWPFF
                     ATRAPLVRPAYHREFLDGLEYYVTSQEVSHRFAVDSLAYPVESAYYRWIHANAPVVWK
                     ADPRTMSGPIIEVRRVPPKVSTRAERDSVFRAAMPAPTAVTRVGLWCADYAVLFGRLN
                     QDDRAEEWADRGLQVRAAQMTPRLLNTLAYAKLQLEKYGEAERVAALGVERSPNDGVI
                     HLYHGIALQALGKPNEALAEYRRCYEISHDARALIRMGSVLAELERFDEALQVLGQIP
                     EDHPDRFAAAREAALILINGMHKPAEGIAALQEAARLARDPAEAARINQEIARIAGEL
                     RAAR"
     gene            16538..16825
                     /gene="groES"
                     /locus_tag="VFR25_06765"
     CDS             16538..16825
                     /gene="groES"
                     /locus_tag="VFR25_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9940785.1"
                     /translation="MNVKPLADRILVRRLEDTEVKRGGIIIPDTAKEKPQQAEVVAVG
                     PGRITDDGKRVALEVKTGDKILMGKYSGTEVKIDGNEYLIMREDDVLAIVG"
     gene            16862..18502
                     /gene="groL"
                     /locus_tag="VFR25_06770"
     CDS             16862..18502
                     /gene="groL"
                     /locus_tag="VFR25_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9940786.1"
                     /translation="MAAKEIIFDEKARQAILIGVQTLAKAVKVTLGPRGRNVVLDKKW
                     GSPTITKDGVTVAKEIELEDRYENMGAQMVREVATKTSDVAGDGTTTATVLAESIFRE
                     GLRNVTAGSNPMGIKRGIDKAVKAVVEELKSISKPVKETKEILQVGTISANGDTEIGE
                     KIAEAMDKVGKDGTITVEEAKSMDTTLDLVEGMQFDKGYISPYFVTDKEGMEAVLDDC
                     YILLYEKKLSNMKDLLPLLEKVAQKGKPFLVVAEDVEGEALATLVVNKLRGTLSACAV
                     KAPGFGDRRKAMLEDIAILTGGKLITEDLGIKLETVKVEDLGRAKRVTIDKENTTIVE
                     GVGKQSDITGRISLIKKQIEDTTSDYDKEKLQERLAKLAGGVAVINVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRCLPAIEKLELTGDEKIGANIVKRALEEPL
                     RQIVNNAGLEGSVIVEHVKKEKKAAVGFNVATEKYEDLFEAGVVDPTKVTRTALQNAA
                     SVAALLLTTEALVTEIAEKKKPAAMPPGGGGGGYDDMY"
     gene            18706..19440
                     /locus_tag="VFR25_06775"
     CDS             18706..19440
                     /locus_tag="VFR25_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940787.1"
                     /translation="MNAWRGVLRSAVVGLAFLFTGGCGPRVDFLHREPDFGYPTLKQG
                     GIAALGGVTTQGSPEGRRSLREPLSQGLETALRGERRDLTVLGVDQVLWEIGDGFRED
                     LLDAFDRDGGLEQEDLALLDSLVGDQARYVALARVEDQWITRDEAEDESEPNETKTTV
                     TVSLTIRVAFVVYDLQAGRSVWSATLTGSDKNSSTFTDKEQGFWATLASAVLGLDDHD
                     DPSAPNAAGTLRKIFSKFAKELPSDD"
     gene            19647..22040
                     /locus_tag="VFR25_06780"
     CDS             19647..22040
                     /locus_tag="VFR25_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9940788.1"
                     /translation="MIRRVLPLLIACSIGVPSGRAAAQMVRADFPVTDGPVYVTALSG
                     NTLYLAGAFTRVGTALGSAVPVDSGSGAALPNFPKFDGPVHAILADGAGGWYVGGEFT
                     KAGGVSRANLARVLSNLTVASWNPGADGAVRALALSGTTVYVGGDFSNVAGQGRSRIA
                     AVDASTGALSSWNPSAIGTAVRTLAVSGATVYAGGDFSTIGGQSRANIAALSTATGLA
                     MAWNPSASTVVDVIVVNGPTVYAGGLFTTIGGQTRRRIAALNASDGLATSWNPNVTIG
                     EVCAIAVSGSTVYAGGSFSTIGGQSRQNLAALNASDGLATSLIATTNGIVRVLQIQGP
                     KLFVGGEFNSFRSEERRYLAALDTGTGLTLPWNPAANNPVHAIAVNGASVCVGGLFTT
                     LGGQIRNRLAAVDAGTGALLPWNPDSDSEVYALVVSGGTVYVGGYFASIGGQLRSFIA
                     AVDAVTGVPTAWDPNSNGQILSLAVANGVVYVGGSYTSIGGAARNSLAALDPATGLAT
                     AWNPGANDWVMSLLPGTGVIYAGGYFTSIGGQSRSYIAALSTSTGLATSWNPAASAHV
                     EALAMGTSVVYAGGAFTTIGGQPRNRLAAVATGTGLATSWNPNADDIVYSMAVNGSTM
                     YVGGSFTTMGVSGRSRVAAVDIASGATTSWNPSADGPVYSIAATGSRVYLGGEFLEVG
                     GLPQSGVASIFPNITAVIEAPSAVETVLGPPSPNPFVQSTSIRLSLQKDAVVTLRVFD
                     VHGRQVAVLADGSYPAGTHVFGWDGRTRAGQAAMSGVYFCELRTATASLKRKLVLVR"
     gene            complement(22047..22532)
                     /locus_tag="VFR25_06785"
     CDS             complement(22047..22532)
                     /locus_tag="VFR25_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9940789.1"
                     /translation="MRTLLGLVALLVALASCSATSDLDTASQAANAKAAIDTLWIHYA
                     EAADRRDPILFESIFTEDASVVYSNAATANGRQAVQEFLSSLYASVDVTNFRVVPEDM
                     KVVGGLAAQSGTFQEEFTEEGKPKVESGRYTLLAEQASDKSWRIHRLVAIADSTILRT
                     P"
     gene            complement(23091..23999)
                     /locus_tag="VFR25_06790"
     CDS             complement(23091..23999)
                     /locus_tag="VFR25_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9940790.1"
                     /translation="MPKPGLVHSHAYFAEIGLHVFPMRKFRMLRDEVVRRGLASETDF
                     AEPVPATRAQVARVHDEEYIEKLVRGTLTELEEAILELPYSKKLVDASFLCAGGSILA
                     ARLARERGAAVNFGGGFHHAFADHGEGFCVLNDVAIAIRDLLHDGTIQRAAVIDVDVH
                     QGNGTAAIFRGDPSVFTFSIHEEDNYPLVKPPSDQDVGLDRGADGKVYLAALGHWVPR
                     ILESHRPDLVAYVAGADPFEEDQLGGLKLTREDLRARDKLVFDAARERSVPVAAYLAG
                     GYAQRVEDTVAIHADMVEEAFRSSAR"
     gene            complement(24005..24949)
                     /locus_tag="VFR25_06795"
     CDS             complement(24005..24949)
                     /locus_tag="VFR25_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940791.1"
                     /translation="MDDQVTLPRPASRPSLPPVSTYLRTVLALGKASIKPGFPALIFL
                     YFYRLGMGLYVALSMDRVQSIDEASSQAVTSAALVQIPAYIPVLVLIYMPFLLLQDGI
                     LRGVATTLGSSVRSVLERMLAFLVCAVAQLVIVAGPPFLLFAGAAILVRTIPTRPEEL
                     TRAIGLLAMVPCLIYAILMLFMLWFAFPALVLDRRGPFRSIRVSFSLVASHFGGILGR
                     LIVVLFFVVLAALVLSFPAEILQVVTAVAGGDHPLLKVATVVWTSAVSAFLFPFVMAS
                     LVVLYRALVPAASGSAVPAGAAVAVPEAHRVSSPFQFE"
     gene            25023..26357
                     /locus_tag="VFR25_06800"
     CDS             25023..26357
                     /locus_tag="VFR25_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HET9940792.1"
                     /translation="MSRAAVKINEWTERWVPGAFTIAWILTLIVIVLALILARASFLD
                     CARAWGDGFWELLPFSMQMCLVMFTGSIVAVSPPVARLLARLAALPKSPRQSILLTAL
                     VSMLLALVHWGLSIVGSAVLVREIGRGRTRVDYRLLVAAAYLGMGTTWHAGLSASAPL
                     LVATPGHFLEKEIGLIPVGQTIFSPFNLVLVAVVIAVFTVITPLLHPSPERTVTVGEA
                     QLAAIRSFAPPRKPAGPLAPAARLEYSPLVNWTFGALALVWLGAYLAKPQHPITLNVL
                     NFVMLGLAVLLHPSAASLVAAAEEAGALVFGIILQFPLYAGMYGIIQSTKLAEILADG
                     FVRIANGSTYPLLVYWYSGIVNYFVPSGGSKWAIEAPYILAAGKSLGVPAAKVVTAYA
                     WGDMMTDIIQPFWAIPLLAAAKLDFREILGFCMVLFCVYAPIVSLAFLFFPR"
     gene            26383..29277
                     /locus_tag="VFR25_06805"
     CDS             26383..29277
                     /locus_tag="VFR25_06805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET9940793.1"
                     /translation="MTTQTTLPLFEEIQLDPEQRAAVEHGDGPLLIVAGAGTGKTAVI
                     ARRIAHLIATKRARPSQILALAFNEKAAAEMQERVDVLVPYGYADVTIRTFHSFGEDV
                     LSGHGMEIGIAPGFSVLDQTAQALFLAERLDELGLARYAPLSDPTRYLSSLATYFGRA
                     KDEPIWPEGLRDRAAWLDEEDSRDRVLELAHAYERYNQLLWEAGFVDFGDLLALTLKL
                     FDESPGTLRRFQERFTHVLVDEFQDTNPVQFEIVKRLTALHRNLVVVGDDDQSIYGFR
                     GAHLKNILDFHDQYPDAKGIVLRRNYRSTKPILEVSRRLIVTNRERLETRIGISKLLT
                     TDVEGEFPRWREFATEAEEAEWIAEQMAEAVASGRRRYRDFAILVRTNRTAEPFLRAL
                     DQRGIPYYFSGSRGLFQRPEVKELVALLRSLFQESRPEYLYLLAAEGYGVAEDDLARL
                     IDRLPMEPLSFKSVLERAAQGTGIFRDLVSDDARGKLGEMLAHLSELHDFAKGKRTGE
                     VLYRYLELRGVLRKLVSSERFEDEARARNIVKFFSIVRSFEKVAVSDRVALFLRHLDA
                     IQDYGEDPAVADIDSALDVVQVLTVHKAKGLEFPVVFLVQLAAGKFPTNNRSGGLDLP
                     APRGDAAADSETHREEERRLCYVAFTRAREELVLTFARDYGGFGRERQPSLFLQEAFD
                     LGKPLPPKRRKRAIEELEEHRPGLPALALVDAHATRDPLELSVRRLEDYETCPLKYRF
                     LHEISVDPILTRDHRVNFGNAMHQAVAHALNQRMLGRAAPFDEVAGVFQRAWRREGYR
                     SEEHERRRFEQGLDAIRDFVAREIDAGPVPSAVERHFRVKLDDVIVTGRMDRVDDQDG
                     EIVLIDYKTTDLDDPEKGDAEAKDSLQLSVYALAYRELTGRTPQRVELRYVLTGDVGR
                     AGPDDKRLEKTRAKIAAIAASIRAGDFEARPSERNCSICACRPICSRSVR"
     gene            complement(29320..29652)
                     /gene="trxA"
                     /locus_tag="VFR25_06810"
     CDS             complement(29320..29652)
                     /gene="trxA"
                     /locus_tag="VFR25_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9940794.1"
                     /translation="MSDAVLQVSDKTFDSEVLQSQIPVLVDFWAEWCGPCRMVAPVVE
                     EVAKLYLGKVKVAKVDVDANQRVASQFRITSIPSLYIFKGGKIVEQIVGAVPKHQITS
                     ILDNVLAA"
     gene            29846..30601
                     /locus_tag="VFR25_06815"
     CDS             29846..30601
                     /locus_tag="VFR25_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940795.1"
                     /translation="MIEVQNLVKTFHDRKKGEIPAVKGVSFTCPSGKIFGLLGRNGAG
                     KTTTLRMLATILQPTSGTARINGHDIHQRPAEVRRSIGYLSGDTKLYDRLTGRDLLQY
                     FGALAGMHGDRIAKRIQELRETFQLDELLDKRVGKMSTGMKQRLSIARVLFHEPQVLI
                     LDEPTAGLDTVAARRLINLIGDMGKRGLTVIFSTHIMTEAERICDEIAIIDRGEILAQ
                     GTVDALCHRNGSGTGESLEDVFIHAVEEVSVER"
     gene            30591..31781
                     /locus_tag="VFR25_06820"
     CDS             30591..31781
                     /locus_tag="VFR25_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9940796.1"
                     /translation="MSAKTFAVFRKEITDTLRDGRSVFAIFIFPFILYPAMLFFSSWI
                     QSKNMEAAKELQVRVGVVGSEALPFVVDSLAAIQGVTPVPMTAVPSDIEGSGVNAVLV
                     IPHDLSAALARGDSTKVELLYKDAENKSSAAERRVRPFLDRLDLMLTLDWARAQGAHA
                     EAGPAFTVESRDTSSKTEVGRYIAALVIPYILIFMITAGAMHTAVDATTGEKERSTLE
                     TLLATSATRAELVLGKVMAVLAAALTGAITGITGLWLTFSVVAKAFPTMESRTLDIAI
                     SPDKLVMIFLTLIPVAVFLSATLVAIGCFARSMREGQTYATYVYMAAVFLGLGSFGEQ
                     TPPLKNFMIPILNTALLQREILTGTVQAAHATAAIGVTVGAAIIMLAMAVRLFSNEGV
                     LFRN"
     gene            31817..33052
                     /locus_tag="VFR25_06825"
     CDS             31817..33052
                     /locus_tag="VFR25_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9940797.1"
                     /translation="MKRDVDTLLRERGLSAALIIKGEIPNFTFRYLVGPPAAHLSTGI
                     LVWKPGQTPHLVHGLMERDSAAATGFERSDFGSHRFRQMLEDEGSRDGAMARLLAKVL
                     SDMEVRGPVLVHGTADVGRYYHVLNRLRSMAPQIEIAQDEDLGLFELARATKDPEEIE
                     AVRGVAAVCSRAYERIRAVIGGGRLEGKKLKDKEGWVTVGRLRREVRRVFFEANLEEP
                     HGNIIAMGRDAGVPHNEGNDADVLEEGRPIVIDLYPAQSGGGYYFDVTRTFCVGRAPA
                     ELKEMHGVVREAVERTMDSLSSEAPARMYQEKVCDFFESKGHKTIRQDERLEEGYIHG
                     LGHGIGLEVHERPNLGGTSQNRDRLSPGSLFTVEPGLYYPSRQIGIRIEDVVYARPDG
                     TFENLTPVPYDLEVAPASS"
     gene            33087..34226
                     /gene="hflX"
                     /locus_tag="VFR25_06830"
     CDS             33087..34226
                     /gene="hflX"
                     /locus_tag="VFR25_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9940798.1"
                     /translation="MSPPAARRRGKERAILAGRAPSRVDHDGDTLTELRLLAETAGAL
                     VLGEVLQRRGSVRSSTFLSKGKIEEIRTLGDEQNANLLLLDDDLSPAQNRNLEEDLKL
                     RVVDRSGLILDIFARRARTREARLQVELAQLEYMLPRLTRMWEHLGRLGGGIGTRGPG
                     ETQLEVDRRRIRERIAKLKRELEGVVRERRVQRRGRRSCHKVSLVGYTNAGKSTLFNA
                     LTRAKVYVEDQLFATLDPTTRVFAAKQGTQALLTDTVGFIRKLPSHLVASFRATLEEV
                     TEADLLLHVVDASEPDPERHIEAVHTVLEEIGALHRPRLLVFNKVDALPDEVAWLGLR
                     ARYPGASFVSAVTREGIPELRDATLARLAEESRPARRVNGEEPRA"
     gene            34223..35458
                     /locus_tag="VFR25_06835"
     CDS             34223..35458
                     /locus_tag="VFR25_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET9940799.1"
                     /translation="MTVAGVGAAVGAERVRRIQEALREDGVPAWLLFDFHGSNPISRK
                     VAGMVSGPDGPKTTRRWFYLVPAQGEPVKIVHRIEPSSLDHLPGEALVYLRWQELRDA
                     LAKAVASVPGPDRVVAMEYSPDAALPYLSRVDAGTVEMVRAAGAGVVSSADLAQRFDG
                     TLAPEAREDHKRTGLVLNGIMAAAFARVRDRVRAGAPLTEVSLQAWILEQFQAEGLVA
                     DDPPAVAVNDHAGDPHFTTRAEHDRPLRRGDLLMIDAWCRRPAPGSVYADYTQVAMVD
                     STVPEKQAEIFAVVRDARDAAIETVRRALRADTSIRGCDVDDAARGVIQARGYGDRFL
                     HRTGHSIGEEVHANGVHLDNLETRDERRLIDGTLVSVEPGIYLPEFGIRSEVNLLIDR
                     GDAIVTAEPYQRELPALLA"
     gene            35458..35865
                     /gene="arfB"
                     /locus_tag="VFR25_06840"
     CDS             35458..35865
                     /gene="arfB"
                     /locus_tag="VFR25_06840"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET9940800.1"
                     /translation="MAARDVPEAEIRFRTSRAGGPGGQNVNKVETRVEALWNLDASLT
                     YTDAQKARIRSELQTRVGADGTIRVVSQRHRSQSRNRDAAVERLRALVAGALRPRRKR
                     KATRPTKASRESRMESKRRRAETKRDRRSIPED"
     gene            36058..37770
                     /locus_tag="VFR25_06845"
     CDS             36058..37770
                     /locus_tag="VFR25_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9940801.1"
                     /translation="MKSFPTQAGPRAIVGAALVILALAVAVPIVWKRPTSEPGVSSGE
                     GSSEPAPPVLGPPSGPRVFRFYQESPTTLDPALAADAYSSTVVAQIYSPLVGLTSNLE
                     PTPQVADSWTISRDGKTYVFHIREGVRFHNGREVTAGDFVYTLTRCFQEPFRSEGLAA
                     GYLDAIDGVPEFLAGKATSIRGIHALDEKRLEIKLSRPYRPLLSALALDQTSVVPREL
                     MDRHGNAILEVKPTGCGPFKYVRRDDRSVVLAANPDYFMGAPAIDSLVFFTPVGDVIS
                     QGANALLDGRATLSTLPIDRVEEFRARAGITMLRWQDLTLAFLGMNATIPPLDDRRVR
                     QAIAFAVDRQAMIHFQPEGKTLATGILAPGVPGYSPATRTYPCDVAKAKALLHQAGYG
                     PDHPLPTLKLYKSVTSNSGVRALDSLLVRSLAEAGIKVEIQYVTWKVLDDAITGRKAQ
                     LFSLSWVADIPDPDTFLRALFYSSSSTNYFKYRNATVDSLLDAARSAEDPKVAQAAYQ
                     KAEERILRDAPFVPLFHPTNFIGLRSNVVGLQMNPLGISTLAMEKLRFAEPKADGDNR
                     SATR"
     gene            37742..40087
                     /locus_tag="VFR25_06850"
     CDS             37742..40087
                     /locus_tag="VFR25_06850"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940802.1"
                     /translation="MATTEARPADAPRRSRGGLQARIFLTFGVIVVGLVTAAVTFFVR
                     SESDSLLRETKKRGFAVARSIAWLSTPALLSYNYIALNQAASRSQAGSEIAYVVIYDK
                     EGQIAADSRSPNSFGQPPRETADFAAQTAREERWSFIEPRRPGDARILEILLPVYVEG
                     YEVKWGTVRVGISLEGLDNEVAALTKNLIIAGFLASILCLLGARFASGRITRPIGRLV
                     GATRAIAQGDYSQRLNLKSGDEIETLAWHFDHMADEVQRQQAEILASRENLARMNQTL
                     EAAVEARTHALTESEAKYRILVDSSPQGLLIVQSGKAVFANPTLEPMTGRPASALVRP
                     DFDPMQLFALESRALVREAIQSPARDRAPVQAEMVHASGRKVFAEVQVVPLVFQNAPA
                     SMLLLSDVTAVRDLQDRLLRGEKLRALGELAAGVAHDFNNNLGIILGRTQLLLMKTSD
                     PEVSSGLNVIRQAAMDGGEAVRRIQQFSRVREDRAHEVLELGEIVVEVVEITRGKWKN
                     ESERRGVKVDVTVEASNPSPILGSRAEIREALTNLIFNSVDALPEGGTITIRCRSDQK
                     ESIVEVADTGIGMSEDVKSRMFEPFFTTKGMSGNGLGLSMVYGIVSRHKGTVEVETAP
                     GAGTVVRMRLPATDLEGTVMPARPRYEAPARARILVVDDETEILAVLREALERVGHTV
                     ETASSGREGIERFRNGAFDVVLSDLGMADVGGWDVARAVREKGPRRVVLGLVTGWGAT
                     ISEEMVNAHAVNFVIAKPFDVDDLVSKVNQSLEAVAMPPAA"
     gene            complement(40068..40571)
                     /locus_tag="VFR25_06855"
     CDS             complement(40068..40571)
                     /locus_tag="VFR25_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9940803.1"
                     /translation="MSESTTRLDGPLVTLEVPGPEHLPTIWKVYLDAPDYFEALSGTS
                     DFDPNTVEEMYEEAIADDSRYYFAIRRTDTKALIGTIEVEVGSDEVPSVLRGLVLSQA
                     ERGHGYGRAALEMVEAFLAEEHAAQSITAEVPMGLDDGERFLEAVGYRERRLKSGDVR
                     WIKRLEA"
     gene            complement(40568..43492)
                     /locus_tag="VFR25_06860"
     CDS             complement(40568..43492)
                     /locus_tag="VFR25_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET9940804.1"
                     /translation="MRLQGLLLGVCLALGATAAHAAPPLDNTGPGARTVSRARALYSQ
                     LHRAPEVDLAEARATTTGIKRVVVVLLQFPDLAADTLAHPPEAYDSLLFSVGTRPTGS
                     FRDYYREVSRNQLDVQGVVTRWYTAPHPYSYYVNSQSGFGAFPTNAQGMAQDAMLLAD
                     PDIDWSQFDGDGDGFVDGVFVVHAGPGGEETGSVDQIWSLKWNLPGGVTLDGTTLYAF
                     TTEPEEWGLNSAYYSAGDLISIGVFCHEFGHILGLPDLYDLDDNQSSGDGEWDLMAYG
                     LYTHSPAHAPGTTPAHMSAWCKARLGWVQPTWVTQDSSGVTIPPVESSGQVFRLWTNG
                     QESNEYFLVENRQPVGFDSTLVRSSVEAGLGPAHGLVIYHIDENRSNNDDPAHKMVDV
                     VEAGGPEQGGHPGNQNLDLPAGALGTKEVCGVSTSVNGNRGDRYDPWPGPLGTTAFNG
                     NSCPSSATSCFHVPSQVAIRNIAEAGGDVMASFFVTGVDVRRLPIVVDDAPLLGTPNN
                     GNGRAEPGEVVHLNFPLLNIGPTATGSLGAKIRPEPAFMSVDTDTITYGSIAASSTSL
                     GSSITATILPTPDPRGVSVSHGVHASAGLVDSDSVQVLVGAKTGICDSFEGTIRRWIS
                     VPLGCDGVNEWHREAGINHTPKGTWAWRLGKPGTLDTYAPAEDARLVSQPVWLAGPAD
                     TLRFWQRYSTEPGVDGLNVEVSVDAGETWTTIVPVGGYSNGFRWTGTQATFAEAVFPL
                     DGLSGPVQFAFHFRSVPPNEGLGWWIDDVQVAGTDDCAATAIAINSFNAIALLDRPGV
                     RLEWNLSENTGSTVQIERASADDGRKTVAILPWESRSGTYVDEHVTAGVDYDYWLTVS
                     RDGEPSSTAGPVNAAVTAGVGPGGAPRVFAIGRVQPNPFARAASFPVSLDQDGRFMVR
                     VYRADGSLVRTLADLSGRAGTSPFTWDGTDDRGNPVGTGVYFIQLRSGHRVRTQKAVL
                     LR"
     gene            complement(43540..44991)
                     /locus_tag="VFR25_06865"
     CDS             complement(43540..44991)
                     /locus_tag="VFR25_06865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940805.1"
                     /translation="MSGYVLEPWAMHLINTISLVILLYFSALQLVLFGLLILSFFDMR
                     RRLAQNFYADYEAVAKSHFTFPISVLVPAYNESAGIVDSVRSVLSLDYPQHEVIVIND
                     GSTDDTLQKLVREFGLERREAVYRRRLTTLGAIGGIYRSSAVPNLVVVDKEHGGKSDA
                     LNMGLNLSRYPLFCTVDADSVFQENALLRVVRPFLEDPDRVIAVGGQVRVANGCKIQR
                     GRVVEPKLPTNILAAFQVVEYLRAFVASRLGLSALNSLLILSGVFSLFRKDVVIEAGG
                     YDASTVTEDMELVLRLHRRMREMNREYSVVFLPDPICWTEVPEGLGALARQRSRWHRG
                     MVHSLWCHKSLIWGKHSGTLRWIGIPYYFLFEFLGPIVELVGYIIIPIAYFMGFLDPL
                     YFWAFLFLAITSGAFLSISAVFLEELSFGLYRSWGDFAQMVAIGILENFSYRILTLLF
                     RLGALVDMLLGRGGWGKQERSSFGRTKPAPAEL"
     gene            complement(44991..46055)
                     /locus_tag="VFR25_06870"
     CDS             complement(44991..46055)
                     /locus_tag="VFR25_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9940806.1"
                     /translation="MIDYQSLIFTLLLLLGIVALTTIAAIVNKIYSNREEDSKQRFLE
                     NLRKRFLLLRSPEGQREALPWIVQSLSGRWSELAAEEISQLELSLRLDVLRTLEESGA
                     VNRHLKDAKSRLKWTRAHALRILGELKLPQSVPTLLSALEDRDPDVRNVAARSLGRMR
                     LQATEEALVALLGKHDQAVSARIAAICIEMGSRTAPLLIKTLRDGSPKARFWAARILG
                     EIKDPRATRSLADALLDTDSDVRSAATWALGHVGDRSTAPLLAPLLHDPIWFVRAHAA
                     EALGKIQDTAYVDALGELLRDRSWWVRKNALDALVSLGEPSKDVLLRTLTSEDRFARD
                     SAAEGLSAMGIVPTPHRETA"
     gene            complement(46057..47661)
                     /locus_tag="VFR25_06875"
     CDS             complement(46057..47661)
                     /locus_tag="VFR25_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940807.1"
                     /translation="MKPAEARRSTPTGRRPFRAAIRAALLIAILAHTAPALAQTAGVS
                     PYDAARARLREDRQAEAIPLLQSELRMRPNNDDARLLLARVLSWQRRYDESLAEYREL
                     LAKKPNDATLRASYARVLAWSGRHTEAAREFRTAIQADSTNLETRVGYARVLSWSGDL
                     AEASTEYQRILAADPRTGDAWLGLASVARWRGAATASDRFVTRAESLHADPEAMAEEK
                     GAVATALRPSLGTGWSASHEREYVDLAPDFTLETDGPYIQGRGTMARRATFTGRVSWL
                     DLVETPGAGSAKNYDLSSVVTRIDGSFLRGYPWQAAASVEYRSFDQNPGTMLFPLGED
                     DTFFGWGARVWRFSGRFTPRGAIHRDYIALKETAPDTAFVPGHVDEYEGGVGWQWSAR
                     GTADALVSRGVYSDDNRRWTAGGGASYRAKTGTPSVTVDSRLTYRDWDFTSRSYFTPL
                     GSVRGSAGVSIAGYEERSAIDYGFRYEFAGINSTNFADIWTHTWSGYLNLTAFGTLPL
                     GLEASYSVDNNSYETWYLGVSGSARW"
     gene            complement(47618..49957)
                     /locus_tag="VFR25_06880"
     CDS             complement(47618..49957)
                     /locus_tag="VFR25_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940808.1"
                     /translation="MRLRDILVPVLILGCALPALAGDAGPRPWKTIRDPRAPIPSPPT
                     RAVLARGDRFEIATPSGSRTVFFRGLNLGAAPPGHYPGEFAITKEDYRRWLRFARSMH
                     ANSIRVYALHPPRFYEALREENEAHPNDPIWLFQEVWTELPDGNDFWDEAYTRGFENE
                     LRLAIDAVHGNAIVDERPGHASGRYEADVSPWLAGWLIGREWEPYAVRETQRLHPDIT
                     EFAGTRFKVEHGTAMEAWLGRVCDLTVSYERVHYARAHAVSFVNWPTLDPMRHPTETE
                     RGGGEAEHDEDAYSVDPSHLVPVDSATLTSGFLGYFANYHAYPYYPDFMNLDPNYQAF
                     HDRHGVCSYAGYLADLKAHTRGIPLLIGEYGVPTSRGVAHQQPQGIHHGGATEIQQGQ
                     DDVRLLEDIAESGASGSLLFSLFDEWYKVNWLVERSERPRDRDPLWLNRLDPEENYGL
                     LAFDPPAPIRIDGAIDDWSGISPYAASDAVQGGAPLRALYVTSDQTSLYLRLDVAPRK
                     PAVRTFGVSFDVLDPARGDRRLPQPLPVSWSRGAELALLVTPPSKGSPRGGAELYQDR
                     AMNVSSFMREWDGRDWRRRGRAPARPVANAAGAYVPLVVETNRERVARNGLFFPAKYF
                     DWGRLECAFDSLGTTVEIAIPWGLVVGDPSSRAALDDKDGTADIETTQTPGVGLLAWA
                     TTAACFRPDSLGPTLAGAKPVKPGEVFVLGPAGSIQSTMNEELTVKTPPQASYSWTTW
                     ETPITKERVKRSADIIQRAFEGMEAREASRSQKVDADRP"
     gene            complement(50036..50722)
                     /locus_tag="VFR25_06885"
     CDS             complement(50036..50722)
                     /locus_tag="VFR25_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9940809.1"
                     /translation="MRLILFDIDGTLLRDGIAAKIAFARALRDTYQTAGPIEGFRFAG
                     MTDPECVTEIMRLAGVDERTILARRDECLRRYVELLAVEVREHQDAHLYPGVRELLER
                     LNKLDSVLVGLLTGNVHQGARLKLRRWNLEPYFRFGAFGDDHENRAVLAQIALEKARV
                     LSGRAFSGSETTVIGDTPKDIACARAIGARAVAVATGSISREALAACDPDALLDSFED
                     HEAALRALFP"
     gene            complement(50772..51863)
                     /locus_tag="VFR25_06890"
     CDS             complement(50772..51863)
                     /locus_tag="VFR25_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET9940810.1"
                     /translation="MPVDIQALLKAIVEAKASDLHISTASPPMARMRGDIVPFQTPPL
                     DPKETRDALYSLLTPAQKADFEERHELDFAIEIPGLSRFRANYFEQRRGVGAVFRVIA
                     TKVPTLEELKGPAILRDLASKERGLVLVTGPTGSGKSTTLAAMIDYVNATFPKHIITI
                     EDPLEFVHERKRCLINQREVGAHTTSFAAALRGALREDPDILLVGELRDLETTSLAIT
                     AAETGHLVFGTLHTNSAPKTIDRLVDIFPADRQAQIRTMLSESLEGVVAQTLIPTADG
                     KGRVAAHEILVGVPALRNLIREGKTAQIPSMMQVGAKDGMVMLDQALKDLVTSGKITA
                     QAALSRSTNPKLFESGMAAAQAAQPAAAR"
     gene            complement(51863..53350)
                     /locus_tag="VFR25_06895"
     CDS             complement(51863..53350)
                     /locus_tag="VFR25_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9940811.1"
                     /translation="MSETGQTKRTLASILIDGGIVTPEQVDAAFQQQLETGRLIGESL
                     VEMGFTTEENIGWALSKQLGIPYVDLASGAADMDLVRRFPESLLRRIQAMPLFQNPEE
                     MVIAMSDPTDSEAVREIEQAAGMPVSLVIGCQGSIRRVLDNVYGPERPSRDKVAARAS
                     AAEAATIAAAAGGEPRHRVVWERSGSTFLLYHVYTARNDRASEIHFVPEKGGIAVHYR
                     TDKGLEPRGVEQPETMLYLRTRLAHLGAPDLEMGELAVQGHVMADLSGDRVHVGVCHA
                     RGASGVTTVLRLHPDRAEAPDLSLLGLSPLGEGEIREFVEGPEGLVIVYGPPRAGGST
                     VLASLASLAMKPERRVVAFEPWPTAPYPAGVTRIQFQAGDPSSVNWRRASVGLGADIV
                     VLDDLLHGDSVAELLDGAAVGRLVFARTGWLDGKALLQCLADSPNGRAVLRDRPFAMI
                     ALPTARHEGSSAWAEPEEAEHRAGILEVTILSDEDRERLWKGQGR"
     gene            53662..55311
                     /locus_tag="VFR25_06900"
     CDS             53662..55311
                     /locus_tag="VFR25_06900"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HET9940812.1"
                     /translation="MKRKSADATGKKRKSGSTEPEAAAPSKKQPKIVRLTATDLAEKQ
                     REISVSEFFTKNRHLLGFDSPAKALLTTVKEAVDNALDACEEAGILPDLLLEIQEISE
                     TRYRIAVQDNGPGIVKAQIPKIFGQLLSGSKFHRLRQSRGQQGIGISAAGMYGQLTTG
                     KPITVISRIGAGKPAHKFDIQIDTRQNRPVVVADHEVEWTPQHGTRVELEIEATYKKG
                     RHSVDGYVEQTAMANPHAQITYVAPTGERTELPRVANELPKPPQEIKPHPHGVELGIL
                     LKMLHDTKARNLRGFLTSEFSRVSAAVAEEIATTAGLSGGMTTQKAAREQAEALYKAI
                     QATKLMAPPSNVLSPIGEELLLEGLRKQIDARFYAATSRSPSVYRGNPFLVEAAIAFG
                     AEQLPADEPITLIRFANRVPLLYQQSACAVTKSVIQTNWKSYGIQQSRGALPSGPMII
                     MVHIASVWVPFTSESKEAVASYPEIMKEIKLALQDVGRKLGIFIRRGMREADAEKKRS
                     YIEKYIPHIGIALQEILSISDKEKDKVCATLKDTLERSRKL"
     gene            55308..56396
                     /locus_tag="VFR25_06905"
     CDS             55308..56396
                     /locus_tag="VFR25_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004590565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET9940813.1"
                     /translation="MSDTNVVPKIKKEAKAVEESILKKKTPSLKFPIRSLANVRYLEK
                     EGYFEIRGKKKERTLTVNTVKTFAQTLRMMALSKTLVETDDMATKREAYYVSKNWDDA
                     RFLEQTESDTVMDDVEALFEVNREQLGFVPEEKGGDVAGTLVVVDKDPDTGKSIRIDC
                     TKFGSGAYSIPISVEDLKFETKAKFILVIETAGMFQRLVKHGYWKKANCILVSMGGVP
                     TRACRRFIRRLADSMKIPVYVFVDGDPYGISNIYRTFKVGSGNAAHINEFFCVPQARF
                     LGITPQDIIDYKLPTHPLKDVDVKRAKDAVKNDPFFQHHKPWVKAMEQLMSMGVRAEQ
                     QALAAHGLNYVMDTYLPEKIAHPERFLP"
     gene            56467..56832
                     /locus_tag="VFR25_06910"
     CDS             56467..56832
                     /locus_tag="VFR25_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9940814.1"
                     /translation="MPIRGVHTMFYTSEPEAMRVFLRDKLGLHGVDVGGGWLIFDVPE
                     ADMGCHPADPNSVHGEPSGTNHISFYCDDVRKTVEELKGRGVEFVDGIVDRGYGLVTH
                     FKVPGGFEVELYQPKYEKG"
     gene            complement(56845..57591)
                     /locus_tag="VFR25_06915"
     CDS             complement(56845..57591)
                     /locus_tag="VFR25_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9940815.1"
                     /translation="MLADLAKRTLVGTRWEEPFRYVHAKLTGHKGSLYDSQTIAVMRR
                     VLRRGGIGVDVGSFEGAYLRHMMRLAPGVKHFAFEPNPDQFAKLQARFPGMNVYPYAI
                     GSEPGSAVFHCMVEHPALSGLSARERDLGNETRRQIDVTVETLDRVLPRDVPVKLVKV
                     DVEGAELGVFRGGLQMLRRQRPHVLFECGLGGADHFGSGAGEIFDVLGEAPLRISLLP
                     DWLAGRSPLTRDQFIEQFERRLNYFFLAHP"
     gene            complement(57664..58377)
                     /locus_tag="VFR25_06920"
     CDS             complement(57664..58377)
                     /locus_tag="VFR25_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET9940816.1"
                     /translation="MDSPKSTARLAGFLYVVMSALMIFSYMYVPSRFLSDDAATTARN
                     IADGASLYRLTILISLVSQILFVVVALTLYRLFRDVDRALARLMVGLVGVAIAAELVT
                     IANRMTPLILLSGESYLSAFTKPQLDALVMGSILWGNNLARILTIFWGLWLFPFGILT
                     IKSGYFPKILGYLLFASGVGYVVTSVTIIAFPEHIAAVSRVMSPLYFGELGMVLWLPF
                     MGARDPAEHAETEAEPAVT"
     gene            complement(58423..58845)
                     /locus_tag="VFR25_06925"
     CDS             complement(58423..58845)
                     /locus_tag="VFR25_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940817.1"
                     /translation="MNRKFWIALALGLLVCLGIGYAWTARLDIPNPKPWWYGRDTVVR
                     VEVWEPGKDLATVAVTVPKKALDYMHAFNLKSEIGLAHDYRINIDELWSKIQRLPKGQ
                     RLHMDEEEADVYIWIEERGTPVDSTWRPPLDSTKAADF"
     gene            complement(58919..59776)
                     /locus_tag="VFR25_06930"
     CDS             complement(58919..59776)
                     /locus_tag="VFR25_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET9940818.1"
                     /translation="MDAFQFLLWPLLACVVIAGIHAYLGFHVVERGVIFVDLALAQVA
                     ALGATIGFLFGLDLHDPASHYLSIAAVVVGAGLLALLKAEKRRIPQEAFIGIVYAVSA
                     AAAILIVDRTPEGAEHIKFMFVGNLLATSPKEVATLAVLYLVIGFLHFIWRRPFFAIT
                     HGRPAEAGLSRAGVRWWDFLFYLTFGLVVTQSVAVAGVLLVFSYLIVPSVIAMLLADR
                     ARSRLIVGWIVAVLVSVGGMLASYHFDLPTGATVVTTFGLALALAMAWRGVAGSRSVA
                     LKPEERSVA"
     gene            complement(59776..60717)
                     /locus_tag="VFR25_06935"
     CDS             complement(59776..60717)
                     /locus_tag="VFR25_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET9940819.1"
                     /translation="MTRFTKRWALPLLTLALMTAPHAARADKKINVVTTTEDLASITN
                     EVGGDLVTVESLSRGYQDPHFVDAKPSYLVKLKKADLFVEVGRDLEVGWVPGLLNSAR
                     NSKILPGGAGFVDASKNIQVLELGGPVGREQGDVHPLGNPHYWLDPENGIIMAAAIRD
                     GLSRVSPANAPTFASRYADFEKRVRAKEAEWKQRAKSIGLTGTRVVTYHRSWPYFARA
                     FGFEVMDFVEPKPGVPPTPKHIQDLTTEMKAQNVKLLLVEPYFDPKMPQQIARNTGAA
                     LVILPPSVGATKEVTSYLGLFDAQLGLIQNALTGKGS"
     gene            complement(60732..61910)
                     /locus_tag="VFR25_06940"
     CDS             complement(60732..61910)
                     /locus_tag="VFR25_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940820.1"
                     /translation="MRYHAWAIAVLAVALVSSAAGFAIAQESSSFSDSTVSAPDAAAT
                     QTTPVDSPASGGVTAPPAGSNLLNPNISAIGWFQGQAGDALDRTDTSAFQLREVELGY
                     QATVDPYSRADFFISVSPDEGIDVEEGYLTLLTLPAGFTAKLGKFRSNFGKFNRTHPP
                     ETPFADRPLAAERFLGEEGLSGIGASVSWLAPLPFYLNFDGEVTNTPDSTSVPAFAEY
                     RGKDLLYLARASTFFDLGETTNLTLGGSYANGANGPAETVDPLAVPDARRAQLYGADA
                     TFRWKNPKRAIYRSLTAQFEWMARDPDDGPRRTGGFAWADYQFARRWHLGARYDWSQA
                     IDAIADDTTTGGLGFLTFTPSEFWSLSLQGQDRDFDGSEMSYFFKTTFNIGPHGQHPF
                     "
     gene            complement(61982..62221)
                     /locus_tag="VFR25_06945"
     CDS             complement(61982..62221)
                     /locus_tag="VFR25_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940821.1"
                     /translation="MALLALLLFGAWLGSAVLHEHPGPPCQICKDLQASQVDVVSPLE
                     PPRLDSPPERLADAVVVESPALLLAAPTGRGPPQT"
     gene            complement(62301..62453)
                     /locus_tag="VFR25_06950"
     CDS             complement(62301..62453)
                     /locus_tag="VFR25_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET9940822.1"
                     /translation="MSIAFPLTLIAVGILLLLKEFGYLHGNLLRYWPALLIVWGVGMI
                     VAARKG"
     gene            complement(62507..63430)
                     /locus_tag="VFR25_06955"
     CDS             complement(62507..63430)
                     /locus_tag="VFR25_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940823.1"
                     /translation="MGFGKLVFGFFLVGMGALLLGAALGYLPHGVWPWLARFWPVLLV
                     GFGLALLANALKNALLGVFALALVAAAFVFGGWWISKHTEMGLPPTQATVINFTNPGI
                     RMLRVQQRAVGGSFAIHTQPGDPDEARMNAKSILGKDMIAHGYEAKNGVGYLVWPTTP
                     IAGDMSLFGSIVDARLPEHQRVSLRSSNYFAFGTINLREDALDEAEIKAISSSVRLAV
                     GQVRPRMINVHGLLSNVEITLPPDAAARVEYDSPLTWHSFTNDFTEHVSGRVKGKAAF
                     WTAEGKGPLTVIHVGGHLMRIRVQRQEPPKN"
     gene            63556..63948
                     /locus_tag="VFR25_06960"
     CDS             63556..63948
                     /locus_tag="VFR25_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940824.1"
                     /translation="MALAAAAIVVATAGCMSGVRSPMEHEITKRVTPEKPEFDIEGWT
                     DSTGTHHKLHGRARIVGDSIQLYQRNYVTNANPTVTTKLVATVLRSDVAEVTVERFDP
                     VKTGMLLATPVLLYGLLILIIVSSGYEP"
     gene            64139..65266
                     /locus_tag="VFR25_06965"
     CDS             64139..65266
                     /locus_tag="VFR25_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940825.1"
                     /translation="MRSKPFAAALLAAAVLTMTSSFAAAQDSIEDKLHFSGYGEVHYN
                     SHELSENGIIENDPPAEVDVHRFVLGWRYDFTPKIRMDAEVDFEHAATEIELEYAYLE
                     YDLAAATSLRAGAVLMPIGPLNEFHEPPNYYSVERPYVESSIMPTTWQEIGIGLVGRL
                     GGGGLGYRAYVVTGLNASGFDTFNGIREGRGHGAEAPAEDLAGVARLEYSTKNGLNLG
                     GSAYYGGADQGDSTLGDVTVTILNADARFRRAGFDLKGVLYHESVGDADVLSNELGTT
                     IGESMLAWYGEAAYDVLRRDREGSSGRALFLFGRYEDFDTNTDVPTGFVANPAANRTV
                     ITGGVSYMPIDKIAFKADVEHWTDDTDADLTRFNLGAAFQF"
     gene            65282..65851
                     /locus_tag="VFR25_06970"
     CDS             65282..65851
                     /locus_tag="VFR25_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940826.1"
                     /translation="MLSKLAKSALLAAVLGNVSIPAAQAGTPRAAQAGGPAAAATEPA
                     PFARVYLAIRGCTSCAHCRTAIRQMVNTKSAGGEARLSADQVEVRYPKPSPVPLKDVI
                     HSLAENRLHDLSLVDVLFEARGTIAPGPNGTATFKLSETGQAFPITIDRAVRRPVDGT
                     AVHLTAVVDGWRTKDKSGLSLAARTIETN"
     gene            65941..66681
                     /locus_tag="VFR25_06975"
     CDS             65941..66681
                     /locus_tag="VFR25_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9940827.1"
                     /translation="MRGKSALVTGGTRGIGKAIALALADMGADVAINFFRSRESAKAA
                     VAEIQAKGVKALAVRANMGNEEQIPPMFEEIKKSFGKLDILVSNAALGHFGNVLEVTD
                     KMWDVAMATNAKALLFCAQEAAKMMPEGGKMVALTSLGSHRYIPGYAAIGVSKAAIET
                     LVRYLAYELAPRKINVNAVSGGFIDTDSLKIFPSYDQIIRESTARTPFGRVGTPEEVA
                     NVAAFLCTSRASWVTGQVLIVDGGYTLG"
     gene            complement(66686..67207)
                     /locus_tag="VFR25_06980"
     CDS             complement(66686..67207)
                     /locus_tag="VFR25_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940828.1"
                     /translation="MASPGGARERIEARRMREARTVDRPWQVWALTALWAIKGAEELL
                     RGILGTGFYVYAQATKGLLFGYALQVAIQSLLFSAVLTAGNFYVMGALWLGKRPARSW
                     GVVFAMLSQIAVLAYLITRPPEFGGMVTLVRYVVVASIVNLGITAFLLFDQKLAAFLG
                     STRLTGWWSARHR"
     gene            complement(67213..67692)
                     /locus_tag="VFR25_06985"
     CDS             complement(67213..67692)
                     /locus_tag="VFR25_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9940829.1"
                     /translation="MPPSHRFDCRLVWTGAREGPIVKYETYSREVRVDFPGKPSLKMS
                     SAAVFRGDPAIHDPEDLLVAALSACHFLSYAALCARAKIQVISYEDDASGVMERAGGR
                     TFKFTDVLLKPRVTLAAGSDAEKARALHEDAHAECFIAASVNFPVRHEPTITVMAAV"
     gene            complement(67713..69056)
                     /locus_tag="VFR25_06990"
     CDS             complement(67713..69056)
                     /locus_tag="VFR25_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9940830.1"
                     /translation="MIRLAMLRWIDTLFPRRLLFEEDRRLLHLFVGRVLSSTGFAIVL
                     PFLSLYLHGTRGIPMTAVGALFLIAALGGALGQVVGGEWSDRSGRKIVLIASQLLRAA
                     AFASLGFAVLTHAPFYVFVLATSLSAFGGRMFEPPSGAMVADMAGADRQAEYYGVLRI
                     AGNLGWALGPAIGGFLAALSYSSLFLVGSGVMLGAASIMAFAVEETSPMHRRRRPRPE
                     PGIAEIPDIGQAAAPPKFQFQESLRALREPNFLRYLLVSTVLFVVMAQLIVTLSVYAV
                     EWGGLTKIQLGTLYALNGLTVVFLQFPAVKLTRTLRLTTSLALGAVLYGLGYGMMGWG
                     RGMALLIVATFVVSLGEIIATPPSLQLVANFSGETTRGRYMGLFGVFNSIGWSLGPVV
                     GGALLDSFHGRPLIVWSVIMALAFLAAVGFLDLRRRLPRSLDRDLETAHARTAVA"
     gene            69078..69959
                     /locus_tag="VFR25_06995"
     CDS             69078..69959
                     /locus_tag="VFR25_06995"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9940831.1"
                     /translation="MLPVDGVVAGYNLVLALVWLGLRDRAPHAWVVALCHLVAAGLPW
                     LFAGARPRGVWRAVREGYPLVGFAVFWGELGVVQRLRGLPPHDEAIRALDLRLFHAHW
                     SDLWMARMPYLALQEVMFFCYFLYYFLLVVPPVAIGVARGWKAFRSVVLAVMLTYLSC
                     FLFYIAFPVYGPRAMAAGMATPAVGFFHTLVERARESGDSLGTAFPSSHVAGVVTMAV
                     MGWRWLGRGWGFVLAVAALAVALSTVYTGNHYVVDAVVGALWVVPLQLWIQPILERRD
                     VGLPRPERADAELPRSL"
     gene            69973..70968
                     /locus_tag="VFR25_07000"
     CDS             69973..70968
                     /locus_tag="VFR25_07000"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET9940832.1"
                     /translation="MSPEPNDIRMDDLRPWRPPARGEALAHYPATDKVLPLGEALARF
                     VPDGSTVALGTCLEQMIPFAAAHELIRRGARDLTLVGPVSDIAFDQLIGAGCARRVAA
                     AWVGNVMMGIAYSFRRAVERGALEVVEFSNFTLAQALHAAAIGAPFIPTRSTLGTDLI
                     ARNPWLQPIEDPLGGGPLLAVKPLHPDVTIVHVQRSDPHGNAQMWGSLGITLDAARAA
                     RAVIVTAEEIVAPEQIRRDPNRVVIPGLVVAAVCESRWGAHPSPVQGYYRRDHEAYSD
                     YHARTKTEEGYAAWRGEWVDAVRDLAGYLARLGNERVASLIPLEHHYAEPVDYGY"
     gene            71009..72451
                     /gene="pabB"
                     /locus_tag="VFR25_07005"
     CDS             71009..72451
                     /gene="pabB"
                     /locus_tag="VFR25_07005"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET9940833.1"
                     /translation="MRPLPPEWTPLRALEIVGRDPYTLFFESGGPAGEASEWTILAFD
                     PLYRLVLRDGRLRRMDPGGSDHELDGHPLDALARAWPAPVHAAEGQAAEIPFISGLAG
                     YLSYDLKDWIERYPSHAVRDLDFPGLSLRFHDVVWAWKRSTGEGWVVSTGLAGDEEGR
                     TANLAFRAGARLAEQWILFKKEMGRTPERTSVPTRPTTVTSTFTRLTYESMVARALEY
                     IAAGDIYQVNLAQRFRVEPVPPALDVYRALRTIAPAPFLAFASLPDGGIASSSPERFF
                     RIKGRTIETWPIKGTRPRGRTPEEDARLLAELRASAKDHAENVMIVDLERNDLGRVCE
                     TGSVRVPALCDVVSHSNVHHLESRVEGTLREDAHPVDVLRALMPGGSITGAPKIRAVE
                     IIDELEPVRRAVYTGAIGYWDVRGGCDWNIAIRTISVVDDVAYFHAGGGVVADSTPAG
                     EYEETLVKASGMMRALGVDAHALDEALARP"
     gene            72448..73446
                     /locus_tag="VFR25_07010"
     CDS             72448..73446
                     /locus_tag="VFR25_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9940834.1"
                     /translation="MKAAVVDAQAPVATSPLHLRDVPDPDPGPGEVRVRVLACGVCRT
                     DLHVIEGDLPPHKLPVIPGHQVVGTVDRLGPGATRFQVGDRIGIAWLRHTDGTCRYCR
                     SARENLCEAQRFTGYDADGGYAEYAVVPEAFAYRIPDGFGDAEATPLLCAGIIGYRAL
                     QRSETPRGGKLGIWGFGSSAHITIQVALARGCEVFVATRGEKHQQLARELGAAWVGGA
                     ADPLPVKVDGGILFAPAGELVPVALHSIEKGGSLAVAGIWLSDIPAMNYERELFYERN
                     LRSVTANTRKDGEAMLAEAAAVRLRPRVTEFPLAEANRVLQLAREDRLSGTAVLRP"
     gene            complement(73443..73991)
                     /locus_tag="VFR25_07015"
     CDS             complement(73443..73991)
                     /locus_tag="VFR25_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940835.1"
                     /translation="MKSLRWLILGLGIGILASGCILTSGQIQLDFAVDDGTVTSNGLV
                     GMPINLSDEKEYQDHKEDIKNLSDLAFLGKFTNNTPNAFTVEVWMTQPTTLPYTDETS
                     MKADPTAVKIWGNFSLAGNQTKQIDWNESAHLFTKAGKDALFEEARHGDGNFTLYAIG
                     SSGTYDFDVKNGALVLVMDAGI"
     gene            complement(74007..74585)
                     /locus_tag="VFR25_07020"
     CDS             complement(74007..74585)
                     /locus_tag="VFR25_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9940836.1"
                     /translation="MKRWLALAAAVALLGAGSAMATEELRVGVGAFYGLSAPVLQDVD
                     ASSFSPGDALGETGGEWGVRVPVRVIPVVTIEPYYAQSSYDDRTETFNGLSYSREGWD
                     RKAIGANFLFANTLATGFKFYPYVGIGSTKLERTDEELSKFTWNFGLGMGFSVNEKIS
                     VHVRSQLDLMVTDETSRKFGDVTAGLQYNFQP"
     gene            complement(74760..75467)
                     /locus_tag="VFR25_07025"
     CDS             complement(74760..75467)
                     /locus_tag="VFR25_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9940837.1"
                     /translation="MSEAPAGVPPVALLIEDELGIRRFLRAALEGQGFTCVEATTGSD
                     GIAQAATRSPEVILLDLGLPDMDGLDVMRRIREWSTVPIIVLTARGKEGEKVDALDGG
                     ADDYVTKPFSVLELLARVRAARRHRLEPDAGAEIVESGSLRIDMARRRVSVHGAAVQL
                     TPIEYRLLATLARHAGRVLTHEFLLREVWGPTSTDQRHYLRVYMAQLRQKLEADPSQP
                     TILMTEPGVGYRMAEQV"
     gene            complement(75464..>76230)
                     /locus_tag="VFR25_07030"
     CDS             complement(75464..>76230)
                     /locus_tag="VFR25_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940838.1"
                     /translation="ADRARHTQSEIEVERARSALLSSVSHDLRTPLTAITGAATTLRD
                     DASLGAITRRELAETIADEAQRLHRLIGNLLDVTRLESGAMRVHKEWHSVEEVVGAAL
                     TRLGPEINGRTIQVSLAGLPLIPMDDILFEQVVRNLVDNAIRHSGNDGPIRIHGRLED
                     GELRLEVCDSGRGLSEEDQARVFEKFYRGGGASHRGGAGLGLAICRGIVEAHGGTIGA
                     ANRATGGAVFTVRLPVQGSPPTIESEADASMREAHS"
BASE COUNT        12854 a        25627 c        25874 g        11875 t
ORIGIN      
        1 cggggcaccg gccggcgcgc tcgaagggct tggtggggtc actcctggag cggcggcggg
       61 cggcggcaga gccgctgcgg ccggcggacc ctccggctct ggcgcggggt caggcggcag
      121 gagcggaagc tgggcagggg gatcgtcgcg cggggtcacg gagccgcatc gtcggcggtg
      181 ccgcgcccca tgtcaagacg gctgctgccc aggacttgcg ccacggtgtc ctgccgcccc
      241 ggacgcccga cgcggccctc tctggaacct ggggccggtc tcgcgagtct ggtataatga
      301 ggtcggcccg gacgccgtcc atcgtccggg agaccaacct tcgaggagcg acacccatga
      361 gcccgaacga ccaggacaag gaaatcgtag aacgcctcat cgcggagacc aatctgtcgg
      421 cgcgtatgga cgcactgatt ggcgagattt gcgacaagct gcgggaatac ggcgtggcgt
      481 caccgcacaa ggccttcaat gatctggtcg accccttccg cgagtacttc atgctggagt
      541 acgaggagga gcggggcatg gaagaagccg aagaggaaga ggcggacgac gaggaggagg
      601 acgagcgcag ctactaggcg ctcggcggcc gctcccgatt caccccggct cgaccggcgc
      661 gctcatccgg actcaccgag cttccggagg agcgccagat tccttcgatc catcaccacg
      721 ccatcgcgtc ctttgggtgt ttccagggtc ttcggcaccg cgtgaagccg cgggtccttg
      781 aggatccgcg cgaacggctc cacgccgaga cgtccgcgcc cgatgtgctc gtggcggtcc
      841 acccggcttc cgcgctcgtt cttcgaatcg ttgaggtgca tccagcggag ggactcgagc
      901 cccaggatgg cgtcgaattc catgaaggtc tcctcccacc cccgctcggt aacgagatcg
      961 tagcccgcgg catgcacgtg gcacgtgtcg aggcacaccc caacgcgccc cgtaagcttc
     1021 actccgtccc ggatccggtg aagctcctcg aaccgtcggc cgagcgtcgt tccctggccg
     1081 gccaccgtct cgaggaggat catcacgtcc ggcgcgtgcg cttccgccag cgcgcggttc
     1141 agcgactccg ccacgcggtc gcagccgtcc cgcacgccct cgcctacgtg cgctccgggg
     1201 tgcgcaacaa gatagggaat ccccagcgcc tcgcagcgtc ggagctcctc cacgagcgcg
     1261 ccgatcgatc gctcgcgcag cacggcatcc ggcgacgcga gattgatgag gtaggagtcg
     1321 tgcgccgcga cggagacgag cccgctctgc tcccacgctt cgcggaacgc accgacctcc
     1381 gggtcgttga gcggcttcgc cctccattga ttgcaattcc tggtaaagag ctggaccacg
     1441 cgcgagcgca cgctgagcgc ccgggccacc gcattcgcga ggccgccggc ggtgctcatg
     1501 tgggcgccga ggagcggccc ttccgtggga gctggggctg ccgcccgcac cttggcgggc
     1561 ggggtgtttc gccggctcga ggggcgcttg cggcgggcct tggggcgagc cggagcgggc
     1621 ttccggcggg acttcgggcg ggttttgcgt cgggttcttt gcactttagg agtggctgga
     1681 ccgagccgat gggtcgttcg ggtaactacg ccaagcgagg atacacgcgg ggaggcggcc
     1741 acggctaagg gcaaacgggg cgcgcgctct ggggtgaggg ggaagcaagc gagcgcgacc
     1801 gccccccgcg tgcgtgcgac gcgtccggtg ccgcggtact gcgtgctctg cggcacggcg
     1861 ctccgcacga tccgggagca cggcgtggcg cgcaaggcgt gtccgaagtg cgggttcatc
     1921 gcctatcgca atccggtgcc cgcgtgcggc gtcgtggtga tgcgccgcgg acaggtgctc
     1981 ctggcgcgcc gcgcgcacca gccgaggatc ggcgcgtggg ggatccccgc cggcttcatg
     2041 gagtacgggg agcatcccga ggtgacggcg gtccgtgaag cccgcgagga gacggggctt
     2101 cgcgtcaagc tcgacgggct gttcggcgtg tacgcgggac gtgacgatcc gcgcacgcgc
     2161 gcggtcttga tcctgtatcg cgcccggatc acgggcggga agctcacggc gggagacgac
     2221 gcgagcgagg tggatttctt ccctctcgac cgtctgcccc gcccgatcgc gtttcgcgcg
     2281 cacacggaag cgctgcgcga tctcaaacgg tggcttgcga gggagcgtcg atgagcgtcc
     2341 gggtccttca tctggccgac atccacctcg gggccgcatt cccgtccatg cgggagcgcg
     2401 ggccggagcg gacgcgcgac ctcctggccg ccttcctccg ctccatcgat ttcgccacgc
     2461 gcgacccgaa gccggtcgac atggtggtga tcgccggcga cctcttcgac acgcatgatc
     2521 ccgaggaagg gctcgtctat caggtcgagg ccgcgatcga caggctggcg caggccggcc
     2581 ttccggtcct catctcgccc gggacgcacg acgcgccttc gtaccgccgc tgcgtgtacc
     2641 ggaggcttcg cctgccgtcg agcgcccaca tcttcctctc gcccacgctc gagccgggcc
     2701 cgctcttcac ggtgggatcg gagacggttc agacctacgg catcgcctac gacgcggctt
     2761 tgtgcgagcg accgttcgcc gacttccgtc cctccggcct cgtcgactat cacctcgcgg
     2821 tcgtgcacgg cgcgctccag gacaatccgg catggaagat ccgtccgacc gacctcccga
     2881 tgacccgccc ggagatcgcg aagtcggggc tccactacgt ggcgctcggc cacttccaca
     2941 acttcgccga gatccgcgag aacggcgtgg ttgcggtcta tccgggcacg ctcgagggaa
     3001 agcggttcgg ggagaacggc ccccggtacc tggttgtcgc cacgctgagc cgggacggcg
     3061 tcacgctcga ccggacgccc tggaacgtcc gcacgctcca cgacgcggtg atcgacctgg
     3121 ggctctccga gcttcgaggc gaggaccagc tcgagtcgcg cctcctcgcc ttcgcggggg
     3181 atcgcgagat cacgcgcatc cggatcgagg gacccgccga cttcgtcttc gacgcggacc
     3241 gcgtgcagag gcggctcgcg cccaagttct tccatctcga gatcgaggac aagacgtacg
     3301 tcgtcaacgc cgcgctcctg acccgctaca aggacgagcc gaccgtgcgc ggcgtgttcg
     3361 tgcgccggat gctcgagaag atcgatcgcg cccccgacat cgcgacgcgg gaggccgcca
     3421 cccacgccct gaggctggga ctcgccgagt tccagaatcc gcgccatgcg ccttgagcgg
     3481 gtgacggtcg agggcttcgg ccccctcacg aatttcgacg cggtgctgga gccgaagcgg
     3541 ctcaacctct tcatcgggcc gaacgagtcg gggaagtcga cgttcgcgtc ggcgatcgtc
     3601 tcggcgatct tcggctgtgt gtccgtggag caggagacgc tgacgcggcc ctggtccggc
     3661 ggcaagcacg cggtggcggt gacgttcacc acgctgaccg gccggttccg cgtgcggcgc
     3721 gacttcgcga cgcatgacgt gcgcgtcgat cgcatggcgg gcgatcagga cacggtcgag
     3781 tccacggtgt tccaggggat cgcgaacccg cgcggcaaga gcgcggagca gctccagtac
     3841 gaagagctcc tgcgcggctg gttcggcttc accgaggcgc ggctcttccg cgaatcgtcc
     3901 ttcgtgcacg agaacggcct cgagacccag gtcagccccg agctgcgcca catcgtttcc
     3961 ggcgccgtcg aggcggacta ccaggagatc gagaacgcgc tgatggagcg tctcgactcg
     4021 ctcacgcgcg agcatccgtt cgatcccagg gcgcggaagc gcgtcaaccg ctcgatcgag
     4081 aatcgcgagg agcgtctcga gcagctccgc gagcggcgga agcgctccga gagcgtgctc
     4141 acggagctca agtcgcgcgt ggcggagcag gacgggatgg aatctaggct ctccgatctc
     4201 aagtccgatc tcgcgaccaa ggagcggctc gtcaacgacc tcgacacgtg gcttcgcctg
     4261 cgcgacgacc agcgccgcca tctcaagcgc gcgccggaca tcggcaccga gctcgtgaac
     4321 gtgcgccgcg cgcggggcca ggtgcaggac atcgaccgga agatccggga ggatctcggc
     4381 tatctcgcca acgcgcccga ggaggcggag gcggatctga tgcggctggg catgcttcga
     4441 tcccagcgtg ccaagcacca gaagacggcc gaggaggcgc gaaagaagac ggacggctcc
     4501 gggtcgggct cgccgatggt cgcggtcctg atctcgatcc ttcttggcgg cggaggcgcg
     4561 gcaggcgcgt ggctcacgct ccacaatccc tgggtcgccg ggggcatcgc gggcgcgggc
     4621 ctcatcggcg cggtgatgtt cggagccatg ctgggccaga agtcccagcg ctcgcgctcg
     4681 ctcgccgaga cccatgtccg cgtgctcgag gagaatatcc gcaccctgtc gcaggagatc
     4741 gaccaggtcg agattcgagt gaatccctac ctggccggac gcacgatgga ggtcgttctc
     4801 gaggacgtga agcgctaccg cgccatgatg caggagcggc gcgaatcggc cgccgtgctc
     4861 cagtcgatgc cagtgcccga gcggctcgag gcggagtcga aggaaatcga cgaggccgtg
     4921 tcgacgctcc gcgcgaagga gaaggcgctt ctccagcagt cgccgttcct ggccccgctg
     4981 cgcgaggacg cggtgaaggc ggccgaagcg gcggagcggc tccggcgcga ggcgacggcg
     5041 cttcggacca agatccaggc ggagcaggat tccttcgacg cggcgctccg gcgcggcggc
     5101 ggtggtccat ccgagaacga cgccgagaat ctcgcgatcc tggacgagac gatcgcttcc
     5161 gaggaggagg cgctcgcgcg cgaggagcgc cagcgggacg cgctcctgat cgcgctcgag
     5221 gtgctccgcg attccgtgct cgcgtaccag gaggagcacg tgcaccggct cgcgcgcgag
     5281 gcgggggaca cgctggagcg gctcaccgag ggccgatacg tgaaggtgaa gctcgacgcc
     5341 gacctcaagc cgaccctcgc gacaccggag cgggaagggc tcccgctcga gtccctcagc
     5401 cggggcacgc gggacgcgtt ctacctggcc ctccgggccg ccctcgcgca ggagctctcg
     5461 gctcgggagc ctctgccgct catcctggac gacccgatcg cccacttcga cgaggagcgc
     5521 cggggctcct tgatccgaat cctcgaggag ctggcgggga acatccaggt ccttctcctg
     5581 acgcacgatc ggggcgtcct gaaccaggtc cgggaagcgc acgtcaccgg cgtgggcgcg
     5641 gtcgcccgcc cgcgggacgc gaagaccaag gtctcggtcc gcccgtagcc ccacgcggcc
     5701 cctagccgcg ccccccaagc ccacaaaccc aagctccgca gttctttcgg ctcaaggtgt
     5761 ggctcgaacg agccgacctt tctcgcgtat gaccaccccg acgactccgt ccagccctag
     5821 ccgtccttcg ccggctccgt cgcccgcggg gaaacccggc tcgcccgagc agcgcgcgga
     5881 ccggatccgc gatcaggtgc tcgcgctcga cgtgaagcgc gtggccgact ggatcggggc
     5941 gctcgtgcgc acgatcaagg cgacgcgcac gtatctctcg aacaacccca cgcttcacaa
     6001 attccaggtc gagctcgagt cgcgcacgtg gtcctgcttg aaagaagcag gcgacatcac
     6061 gctcgtcgtg cagcagttcg atcttctcta cgagaactac tccgtctacc acaacgcgga
     6121 ccggaacgat tccctcgcct tccgcttcta cggcgacggt gttcgcgtga tcacgttccg
     6181 cgaagggctc gagccgcacg aactgcgcgc attcatcgac gtgctgaagc gctcgatgga
     6241 gacggcgcag gtgcaggacg acgtcgtcac gctcctctgg gagcgggact tccggcacat
     6301 cgagtaccag ttcatcccgc tcgaggatct caccgaggag tcgggcgaga tcctcaatcc
     6361 cgaggacgaa gaactggcgg tcagcgaagg cggcgggatt ccgtggccgt ccggcttcgc
     6421 gccgtcgtcc gaggacgaga tgcccgctca ggtcgtggac gtcgcgccgg aacagtcgac
     6481 ggacgcgccg accggcgaag cgtccacggt cacgggcccg actcgggagc gctccgacga
     6541 ttgggagccc acggtgcacg cggcgcccgc gtacgacagc acgatcggcg tccagttcgg
     6601 gctcaccgac gaagaaaacg ccacgctcga ggtgtcgatc cgcgaagagg aagggcgcca
     6661 gctcggccgc gaagtgctcg agatcgtggc gacgatcctc gcgcacgagg acacgccggc
     6721 cgggttcctc gactccgcga ccgcgttcca gcgcttcatc gagctcgcga tcgaggaggg
     6781 cgaggtggcc cgcgccaacg acctcgtcgc ccgcctccgg agcatcgcgg ccgattgcgc
     6841 caagaagaac gcggagttcc cggggctcgc cgagcaggtg gtccgcgaag tcgggcgtcc
     6901 cagcttcctc ggcctcatgg ctccgacgct gaacgcgcgt cccgagatcg atcccggcat
     6961 cctcaccaac ttcctggtcc agctcggccc gtccgcggcg cccgcgatct gcgatctgct
     7021 ggggcaggtc aatcagatga agttccggcg ggcgctctgc gaggcgctcg cgatctcctg
     7081 caagaacgac gtcgaggtcc tgatcgagcg actgggcgac acgcgctggt acgtgatccg
     7141 gaacatcgtc tacgtgctcg gccggatcgc gcaccagggc gtggagcgcg cgctcgaccg
     7201 ggcgctccac cacgaggacg tccgcgtgcg gaaggaagcg gtccgcgcgc tcgggaacat
     7261 cgagagcccg acgagccgcg cgtacctcgt gtccgcgttc cgggacaccg atgccggcgt
     7321 tcgcatccag gcagccatga cgctcgcgat gcgccgggac gaccgcgcgt cgcagagcct
     7381 cctcggcgtc atccagggaa ccgagttcgg ccggcgcgac acgaacgagc ggcaggcgtt
     7441 cttcgaggcg ctcggccgct gcggctccga cgcgctcgtg ccgcggctcg accagatgct
     7501 cacgaagggg ggcctcttcc gctcctccaa cgaagaggag cgcctccacg ccgcgctcgc
     7561 gctggcgtgg ctcggcacgc cgaacgcgct ccaggtgctg aaccgcgaga tcaccagcaa
     7621 gcgcgagccg atccggaagg ccgtcgagac ggcgctctcc gcggttcgga acgccggctt
     7681 ggcgacgaaa gacccggccc cgggcgacga ggaggacgag tgagcggcgc tacccgcacg
     7741 acgcttccga cgacgcgctc gaccagggac gacacctcca gccgcctggt tctccagggg
     7801 cggagcctct ccatccagct ctgcgtcctc ttgaagaccg cgcgcatcca cgacgtcggg
     7861 aacctggcgt tccaggggcc gatgtccgcg atcatcgaga ccgtggacca gatgtggacg
     7921 gcggaaggga agttcaagct ccaggcgatc ggcgacttcc tctacgtgaa ccagctccgg
     7981 ctgcgcgtcg atgcctcgtc ctatcccagc taccagtacc tgatcgacga gctcaaaacg
     8041 cgcgacctga gcggcttcca gttcgcgggc cggctcacgc cgcccgaggt gaagaagttc
     8101 gtccggctct tcctcgacgt cgatcccaaa tcggagagtc cgttcgacga cttcaccgac
     8161 tcgctcgcga agctctcggt ccagaacatc gttccgatcc ggacggtgat gcccaaggaa
     8221 ggcgccgtca cggccgagga acgccgcgac ggccggaagg cagcgaagcg gggcttctac
     8281 cgggcgatcg agtccgcgcg caccgtgatg ctgagcgcgc gcgatcaccg gccggtggat
     8341 ctaaggaagg cgaagcgcgc ggtccagtcc atcgtcgatc tcatcatcga cgaggagttc
     8401 tcgctcctcg ggctcaccgc gatcaagaat cacgacgagt acacgttcca gcactgcgtg
     8461 aacgtgtcga tcctgtccat cgccgtcggc cagaggctcg ggctctcgaa gaagatgctc
     8521 ggagaactgg gcgtggccgc tctgctccac gacctcggca aggcgtccat cccgccctgg
     8581 gtgctcaaca aggccgggaa gctcacgccc gaggagtgga agctcgtcgt ggaccatccg
     8641 gtccagggcg tgaagatgat cgcccgcatg cggggactga acgacctcgc gctccgctcg
     8701 atgatcgtgg cctacgagca ccacatcaat gtcgacaaga gcggctaccc gcagctcgaa
     8761 gggcacatgg atcagtcgct cttcagccgg atcgtcgcga tcgtggactg cttcgacgcc
     8821 atgacggcgc accgcgccta ccggaagagc gcgtttcctc cgtacgcggc gctccaccag
     8881 atcatctcgc agaaccggaa caagttcgat ccgctcctca tcaaggcgtt catcaacacg
     8941 gtggggatgt acccggcggg gacgacggtg ctcctcgaca cgaacgagat cgcggtcgtg
     9001 atctcgcaca acgcgacgga tatcttccgt ccgagggtgc aggtcgtggc ggactcggac
     9061 cggaagcccg tccaggacgg cacgatcatc gatctcaccg cgcgcgacga ggccaacgcc
     9121 acctatctgg tcaccatcgt ctccgcgctc gaccccgagg agtacggcat caacatcgcc
     9181 gacgtgctga cgtaggctcg gctcgctact tcgctacgac gaaccttccc cgctgaaccc
     9241 ccgaagatag ctccaggctg tagaagaaca cgcccgacgg cagagcctcg cctcgctcgt
     9301 tccgtgccgc aaacggcact tcatgcgcgc cagccccata cgtcgctccg tggatcaagg
     9361 ttcgaacaca ccggccggag acatcgtaca acctcagggt cacggccccg ggcgatggca
     9421 gcgtgaactc gagcatgccc tccgggttga gcgggttggg tgagatccgc gccggccgaa
     9481 tctctcccgt accgaggacc gtgagcattg ccgaggcatg gattcgtctg tggtcaaaga
     9541 gccgcccgtc gatcgccacc tccacgtcca tccggccccg gaagggctcg aagagcgtcc
     9601 ggaaatcctc ggagcggaac gaggcggcca gctcacggat tccgtcgtga ttcgtatcgc
     9661 cctccggcat cgtcttgcct tggacggccg cgatgtgggt cgtcgagcct ggacccgcgt
     9721 aggacagagt cagggtcgac gggtccacgt cctcgttctc gtacgaggcg cccacgggct
     9781 ggacctgaag cgtcaagtca cccgaccgcg cgagcgggat gggatggcgt ccgccccgaa
     9841 ggaacgctgt cgcatcgagg ctggtcgacg tcgcgaacac gtagatgcgg ccatatctct
     9901 gattcggcga tccgacgaaa atctcggcga cgccgtcccc gtcccagtcc gcggccgtca
     9961 agatcgtgcc gctcgggatc gaggtcccat cgtatcggag cgtgacatca ggcaccgcgt
    10021 cgggtgaatg gccgccgaaa tacacgacga tctcgaaggt tgtggctacg aggatgtccg
    10081 gaaccccatc gccgctcacg tccttccctc cgaagaccca ccggccgaag gtgctgtccg
    10141 attcactcgc caggaccagg tcaggcgacg ggtaggagac cggcgatgcg gaaccgaagt
    10201 agacgtaggc gcggccggag gagatgctcc cgtcgtcgtg gacgaccggc gcgccgacaa
    10261 ccagatcatc gaatccatcg gcgttgaagt ctccggcgct cgacatcggc tgagtgagcg
    10321 agggtatcga cagcggagtc atggcgagga aggcgtcggg gaccgtgtcg gtgaccgctc
    10381 ccccgaggaa cagcgtcgcg cggccgtgtc caagaggaca tggcggcgaa ccgaatccgc
    10441 tctccccgac gaggacgtcg ctgtacccgt cgccgttcat atcgcccgcg ggtgtcatgc
    10501 tcgtcgggaa cccccacccg acaatgggag gcaggggcgg gctgaacgac gcgtcgggtc
    10561 cggcgtcaag cgacgccgat ccggaatgga tgtagacccg cccgaagctc tgcggatcgg
    10621 cccgtttccc gatcgcgagg tcggcgaatc cgtcccggtc cagatccatg accgagaggg
    10681 ccgatccaaa aaactgacgc gtgggagctt ggcggggctc ggggtcatgg agcacgagat
    10741 cggcctgcgc gtcggggacc ttgctcccga ggtagacgta acaagcctgt tcggcgtcgt
    10801 gaccggggat tgcgagcgga tcgccgagga cgatgtccga gtagccgtcc ccgttcaggt
    10861 cgccggctgc gatgacggac ccatatccgt acgtgagcgg gttcgagggc ttggccaata
    10921 gaaggtcgag cgtggtgtcc acctgagcac tacccaggta caggaaggcc gaaccgccaa
    10981 accatcccgg gacagcagcc aggagatctt catacccatc gccgtcgacg tcgccgaccc
    11041 cggcgatgaa cgaaccgaat ccgatcgacg gtcccaggat ggtggcgatc aagtgaggat
    11101 ctggtggtgg aggcggaata tccagcgggg aggccgccga tccaggaatg aggaacgggg
    11161 cgaagagaag aatcgcgagg cccgggaaca agagaatggg acgcatgagc gtcatctccg
    11221 cgacgtgcgc cggcccgcgg gggagatatc gaatatctta ctcccctcgt tcgcggcaag
    11281 ccatgcggaa gccttcgtga ccctcgcgag gcccgtcttg acccccgcgc aactccccgc
    11341 taaactctta cggtttcgct actttcctcc tgaattgagg agaccctccg ttgagcctgc
    11401 gcccaccgcg cgccctcatc gtggacaagg actcgtccga gacagccgag ctgaaagcgt
    11461 tcctgtccgc caacgacatc gacgtcgagt gggcgaagga cggggagcag gcgttcaaca
    11521 tcctcgatgg cgccagcgcc aacggctccg gcgagacggc gccccccggc cccgaggtcg
    11581 tgatcaccga gctcaaggcg caccgcgtgg acggcatgcg cctcctcgag gtggcgaaga
    11641 agcggaatcc cgaggtctgc gtgatcttca tcgcggacca gggctcgctc gagctcgcga
    11701 ccgaagcgat gcggcaaggg gcctacgact tccagcaccg tcccctcaac cgggacaaga
    11761 tgctcgccgt catccgccgc gcgctctcgc accagcagct cgtgagccgc gcggaggacc
    11821 tcgccgaccg cctcgaccgg aggctccgca tcccgagcct cacggggcag tcgcgcgtga
    11881 tggaagacct caaggacaag atcctccaga tcgcgccgac gcgcgccacc gtgctcatct
    11941 acggtgagac cgggacgggc aaggagctga tcgcacaggc catccaccag ctctcgcccc
    12001 ggaaggacga gcgcttcgtg aagcttcact gcgcggagct ctcggccaac atcatcgaga
    12061 gcgagctctt cgggcacgag aaggggtcgt tcaccggcgc cgatcagcag cggaaagggc
    12121 gtttcgagat cgcggaccac gggacgctct tcatcgacga gatctccgag atctcggccc
    12181 cgatccagac gaagctcctc cgcgtgcttc aagaccggca attcgagcgc gtcggcggga
    12241 acgagaccgt gaccgtggac gtgcgcgtga tcgcggcgac gaaccgccgg ctcgacctca
    12301 tggccgcgcg ggggttgttc cgcgaggacc tcttctaccg gctcaaggtg atccagctcg
    12361 aggtgccgcc gcttcgcgac cggcgggagg acatcccgct cctgatcgag gccttcatcc
    12421 gggagttcga caaggagcac ggacgcaagg tcaccggtgt cacccgcggg gtggtggacc
    12481 ggatgatgga gtacgaatgg ccgggcatcg tgcgcgagct caagaacacg atcgaagaga
    12541 tggtgatctt cgcccaaggg cggaagctcc tcgacgtgtc cgatctcccg atcacgatcc
    12601 gccagcagcg cgcctccgcg gcgacggcca tgcccgtgtc ggtgggcatg tcgatgaacg
    12661 agatcgagcg cgcggcgctc gaggcgacgc tccgctccgt gggctacgac aagcagcgcg
    12721 cggcgaagat cctcgggatc ggacttcgga ccctctaccg gaagcagaag gagtacggac
    12781 tgtgagcgag cggaccgaga ccgcggctcc ggctctgggc ggacgcccgg cccagggcat
    12841 ctccgaggtc atcctggtcg tgaaggacgt cgcgcggtcg ttcgcgttct atcgcgacgt
    12901 cgtcgggctc gccgtcgaca acgccggcaa cgagaagttc gcctggctct gggcgggccc
    12961 gccggggaag tcgcaacgga tcgggatcac gacggggccc ttgtcgttcg gcgcggcgca
    13021 cgtgaaaggg ccgctccatt tcgcgttcgg gaccgaggac gagcgcatcg aggagctcaa
    13081 ggccgcgctc gagcgccatg ggctcgaggt ggaggggccg gtctcgttcc cgttctgggg
    13141 cgcgcgatcg atctacttca gcgatcccga cggcaatcgc gtcgagttct gcggcttcgg
    13201 cccgacgcag ggcacggggc gggtccacat caaagccaag cgctgacctc cgtcgcggca
    13261 gactctctcg ttaggctcac tacaacgacg acaagactag ggcgatgaag atgaccgagg
    13321 ccgccagcac cactccggtg ccggccaacc ccgccgccga cccgagctga gctccgacat
    13381 acgacccgcg ctccttcacc tccccatggg tatcccgtcg cttggtctcg aagatctggt
    13441 acgcgatgcc gaacgaggcg gagggattga caccgactcg cagaatcggt gcgggtgtca
    13501 ccagctcctt gttgtcctcc tcatgcccga acaggatcag gcccggcgcc aggtccacgc
    13561 tcgcgctctt gccgagccag tatcgaagcc tgccacgcaa accgaagtgc gttctgtcgg
    13621 agccgtcccc tcccgcgaac agggagccgc cgatcgagat gcggctcgtg acgttgtgcg
    13681 ccgctccccc gtcgaaggag aggtagtagc gctcctcgga cgcgtgacgc tcatcctcgt
    13741 tcagccggcg gagcaccgtc agctcggtga ctccgaacgt gccgcagtgc gcccggggtc
    13801 cagcgtgcca ggagaacgac ttccttggcg gcgcgtcccc ctccgccagg tcgagcacag
    13861 caccgcaccc cgcagcggag gcgacaccgg cggccagcgt gagccctcga agggttcgaa
    13921 ggccggcacg aatcaaggcg acgttggcgc tcatgcccac tccaacgcca ccggtcatca
    13981 aaaacgcacg ccgcctaccg cgccgcccgc agctccccgg cgatccgcgc gatctcctgg
    14041 ttgatcctcg ccgcctcggc aggatcccga gccaggcgcg ccgcctcctg gagcgccgcg
    14101 atcccctccg ccggcttgtg catcccgttg atcaggatca gcgccgcctc gcgcgccgcg
    14161 gcgaagcggt ccggatggtc ttcgggaatc tggccgagta cttgaagcgc ctcgtcgaat
    14221 cgctccagct ccgcgagcac cgagcccatg cggatcagcg cgcgcgcgtc gtgagagatc
    14281 tcgtagcacc tccggtattc ggcgagcgcc tcgttcggct tgccgagcgc ctggagcgcg
    14341 atgccgtgat agaggtggat gacgccgtcg ttgggtgagc gctccacgcc gagcgcggcc
    14401 acgcgctccg cctctccgta cttctcgagc tggagcttgg cgtaggcgag cgtgttgagg
    14461 agccggggcg tcatctgcgc ggcgcgcacc tggagtcctc gatcagccca ttcctccgcg
    14521 cggtcgtcct ggttcagccg gccgaagagc accgcgtaat cggcgcacca gagcccgacg
    14581 cgggtcaccg cggtgggcgc gggcatcgcg gcccggaaca cgctgtcccg ctcggctcgc
    14641 gtgctcacct tgggcgggac gcggcgcacc tcgatgatcg gaccggacat cgtcctgggg
    14701 tccgccttcc agacgaccgg cgcgttcgcg tgaatccagc ggtagtacgc cgactcgacc
    14761 ggatacgcga gggagtcgac cgcgaagcgg tgcgacacct cctgcgacgt gacgtagtac
    14821 tcgagtccgt ccaggaattc acggtggtag gccgggcgca cgaggggcgc gcgcgtcgcg
    14881 aagaagggcc agatcacgat cgaccgctcg tcggcgcgga acaccggccc gtacagctcg
    14941 acccccatcg gggcggtggc cgggatgttc tcgaggatcc atcgccgcac gacgtgccgc
    15001 gtatcgggaa gcgcgagcgc gcgccggagc ccgacgtact cggggagcgg ccacgcgacc
    15061 gcggtcaggg cgaggagggc cggcgcgagc acgaccgcgc ggcgtcctgc ctcccgggcc
    15121 atgagccagc gccccgcttc gatcacggcg agcgcggccg cgatgaagag caccggcatc
    15181 gcgaccagca tgtaccgctc ctgcgccgcc ttgagcacgc cgttcgcgag caccatcacc
    15241 gctgggatca aagcgatggc ctgcgccgcc cgtccgcggc gccagaggta gccgctcccc
    15301 gcgatcccga gcaggtaggc cgcccacccc atcgcgtgcg gcagcgacac ggtgatgtac
    15361 gtggggagcg agaacggaaa cgtggtctgc ccgacccaat ccgagaagag cgcgctccgc
    15421 tgcgtgccga tgtcgcggag ggtcgtcttc cagtcgatga acgtgaacgg agtcgccacg
    15481 acgagcgcgg cgaagaccgc caggagcgcc agcccgtacc cgcggctccg ccagaagtcc
    15541 cgtccgtacg ccaccgccag cggaatcacg aggatcaacg gcacgtactt gcatgcgccc
    15601 gcgaagccga tcatcgcgcc ggcgatgatg gcgtcccgct cggagcccgt ttcggcgagg
    15661 cgagtcatcg cgatggcggc gaggaggacg aagaggagcg ccaggagatt ggggtcggcg
    15721 acgtgctgcg acgtctggat gtggagcggg ttgagcgcga ggaggagtcc gcccaggagc
    15781 cccacctcgc gtccggccag ccgctggccg agccggaacg taaggaacac cgtgaccgct
    15841 cccacgagca cgcccgcgag gcgcgcgagc aggaagcact gatccacgtg ctccgtgaag
    15901 aactcgacga agtccctcgc gctgccgtcc ggatgcgaca ggccccacca cgccttgaac
    15961 gcgatctggc agacgagcgc gaagtagaag ctgaaggcgg gccagccgcc ggtgtgcggg
    16021 ttgaggtccc agtgccggag gccgccgctc cacatgaccc aggcccggcc catcgtcgtg
    16081 cggtcggtct cctcgaccac ggagtcgtcg aggacgttga tcttgagggt cgggtcgggc
    16141 agacggtcgg cgacccccca gagccggatc gcgatcgaga gcgccacgag caggagcgcc
    16201 agccggtccg ccttccgcag gcgatccatc caggacgggc ccttggcctt gggcggagcg
    16261 gggtgaggcg ccttagctcc gcgcgcgcct ctagcttgca gccggttctt ctgtcgcgag
    16321 ccacctgcca tatgggcaat ctcctcgacc gaccgggatc gcgccgcggg ggcgcgccga
    16381 aggccgggtc agtctatgtg gatgcctgcc aaggggctag tcccgaaagt tagcactccg
    16441 gcatggggat tgcgcatcta aggctcacgg gaaggggcag aggctcctcc tttgcccatt
    16501 tgtcacccaa ccacaatcca caaggagtac tcgaatcatg aatgtgaagc cgctcgctga
    16561 ccgtattctc gtccgccgtc tcgaggacac cgaggtgaag cgcggtggga tcatcattcc
    16621 cgacaccgcc aaggagaagc cccagcaggc cgaggtcgtg gccgtcggcc cgggccgcat
    16681 caccgacgac ggcaagcgcg tcgccctcga ggtgaagacg ggcgacaaga tcctcatggg
    16741 caagtactcg ggcaccgagg tcaagatcga cggcaacgag tacctcatca tgcgcgagga
    16801 cgacgtcctc gcgatcgtcg gctaacgcga gcctctctca ccgataaagg agctagaacg
    16861 aatggctgcc aaggaaatca ttttcgacga gaaggcccgc caggccattc tcatcggagt
    16921 tcagaccctc gcgaaggccg tgaaggtgac cctcggtccc cgcggccgga acgtcgtcct
    16981 cgacaagaag tggggctcgc ccacgatcac caaggacggc gtgaccgtcg cgaaggagat
    17041 cgagctcgag gaccgctacg agaacatggg cgcgcagatg gtgcgcgagg tcgcgacgaa
    17101 gacctccgac gtggccggcg acggcacgac caccgcgacc gttctcgcgg agtcgatctt
    17161 ccgtgagggc ctccgcaacg tgaccgccgg ctcgaacccg atgggcatca agcgcgggat
    17221 cgacaaggcc gtgaaggccg tcgtcgagga gctcaagtcg atctccaagc cggtgaagga
    17281 gaccaaggag atcctccagg tcggcacgat ctccgcgaac ggcgacaccg agatcggcga
    17341 gaagatcgcc gaggcgatgg acaaggtcgg caaggacggc acgatcaccg tcgaggaagc
    17401 caagtcgatg gacacgaccc tcgacctcgt cgaggggatg cagttcgaca aaggctacat
    17461 ctcgccgtac ttcgtgacgg acaaggaagg catggaagcg gtcctcgacg actgctacat
    17521 cctcctctac gagaagaagc tctcgaacat gaaggatctc ctcccgctgc tcgagaaggt
    17581 ggcccagaag ggcaagccgt tcctcgtggt cgcggaggat gtcgagggtg aggcgctcgc
    17641 gaccctggtc gtgaacaagc tccgcggcac gctctcggcc tgcgccgtca aggcgccggg
    17701 cttcggcgac cgccggaagg ccatgctcga ggacatcgcg atcctgacgg gtggcaagct
    17761 gatcaccgag gacctcggga tcaagctcga gaccgtgaag gtcgaggacc tcggccgggc
    17821 gaagcgcgtc acgatcgaca aggagaacac cacgatcgtc gagggcgtcg gcaagcagtc
    17881 cgacatcacg ggccggatca gcctcatcaa gaagcagatc gaggacacga cctccgacta
    17941 cgacaaggag aagctccagg agcgtctcgc gaagctcgcc ggcggcgtgg ccgtcatcaa
    18001 tgtcggcgcg gcgaccgaga ccgagatgaa ggagaagaag gcccgcgtcg aggacgcgct
    18061 ccacgcgacc cgcgcggccg tcgaggaagg gatcgttccg ggcggcggcg tcgctcttct
    18121 gcgctgcctg ccggccatcg agaagctcga gctcacgggc gacgagaaga tcggcgcgaa
    18181 catcgtcaag cgcgcgctcg aggagccgct ccgtcagatc gtgaacaacg ccgggctcga
    18241 gggatcggtg atcgtcgagc acgtgaagaa ggagaagaag gccgcggtcg gattcaatgt
    18301 cgcgaccgag aagtacgagg atctcttcga ggcgggcgtg gtcgatccga ccaaggtcac
    18361 gcgcacggcg ctccagaacg cggcgtcggt ggcggccctt ctcctcacga ccgaggctct
    18421 ggtcaccgag atcgccgaga agaagaagcc ggccgcgatg cctccgggtg gtggcggcgg
    18481 cggctacgac gacatgtact agaacttccc acggccggcc tcggccgggc gcgtccgcta
    18541 tggaaggcta gcgcgctgaa gaggtgatga acggaagggg gtggagcgaa agctccaccc
    18601 cgtttctatt tctggtatcg cccgttcgct ttggactcca cgatgcgttg ttccccaagc
    18661 aaggagatag cttccccgca aaccgtccgg agggagaggc gaccgatgaa cgcatggcgc
    18721 ggcgtgctcc gatctgccgt cgtgggtctg gcattcctgt tcaccggggg atgcgggccg
    18781 agggtggact tcctccacag ggagcccgat ttcgggtacc ccaccctcaa gcaaggaggg
    18841 atcgccgcac tcgggggggt caccacccag gggagccctg agggccggcg ctcgctccgc
    18901 gagcctctat cccagggtct ggaaaccgct ctccgggggg agcgccgcga cctgacggtg
    18961 ctcggggtcg accaggttct ctgggagatc ggggatggct tccgcgagga cctgctcgat
    19021 gccttcgatc gagacggcgg gctggaacag gaggacctgg cgcttctgga cagcctggtg
    19081 ggggaccagg cccggtacgt cgcgctcgcc agggtcgagg accagtggat cacccgggac
    19141 gaggcggaag acgaatccga gcccaacgag accaagacca ccgtgacggt ttcgctcacg
    19201 attcgcgtcg cgttcgtggt ctacgacctt caggccggtc gctcggtctg gagcgcgacc
    19261 ctaacgggct ccgacaagaa ctcctcgacc ttcaccgata aggagcaggg tttctgggca
    19321 accctcgcga gcgcggtcct gggcctcgat gatcacgacg acccgtcggc tcccaacgcg
    19381 gccggcaccc tccggaagat cttctccaag ttcgccaagg agctgccgag cgacgactag
    19441 ccgctccgca cccgtcctcg ttctgccccg atggctcgac cgagccacga cacgtcgcga
    19501 gatcacccta cgcggcggcc ccgaaatcgc gatctcaccc actcgtgcgg caggctgctt
    19561 cgagccattg caagacattg ccgcacagcc acttcgtaag agttcgacca tgatcccgtg
    19621 gtcgattcct tgcattcgac tcaggcatga ttcgccgggt gcttccgctc ctgatcgcgt
    19681 gctccatcgg tgtgccctcg ggtcgcgccg cggctcagat ggtccgagcc gattttccgg
    19741 tgacggacgg ccctgtctac gtcacggcgc tctcgggaaa cacgctctac ctggccggcg
    19801 cgttcacgcg cgtcgggacg gcgctcggca gcgcagtccc ggtcgattcc ggcagcggcg
    19861 cggcgctccc caacttcccc aagttcgacg gaccggtgca cgccatcctc gcggacggcg
    19921 cgggcgggtg gtacgtcggc ggcgagttca ccaaggccgg cggcgtgtcg cgcgccaacc
    19981 tggcgcgcgt actctcgaac ctcacggtcg cgtcctggaa tccgggagcc gatggtgctg
    20041 ttcgcgcgct cgcgctgagc ggcaccacgg tgtatgtcgg cggtgacttc tccaacgtgg
    20101 cgggccaggg tcgaagccgg atcgccgcgg tggatgcatc cacgggagcc ttgagcagct
    20161 ggaatccgag cgccatcggc acggcggtcc ggacgctcgc ggtgagcggc gcgacggtct
    20221 atgccggcgg cgatttcagc actatcggtg ggcagtcgcg cgccaacatc gcggcgctga
    20281 gcaccgccac cggtctggcc atggcgtgga atcccagcgc gagcaccgtc gtggacgtca
    20341 tcgtcgtgaa cggccccacg gtctatgccg ggggtctctt cacgaccatc ggcggacaga
    20401 cgcggcgtcg catcgccgcg ctcaacgcct cggacgggct ggccacgagc tggaacccca
    20461 acgtgacgat cggcgaggtt tgcgcgatcg cggtgagcgg ctccacggtc tacgcgggtg
    20521 ggagcttcag cacgatcggc gggcaatcgc gccagaacct ggcggcgctg aacgcgtccg
    20581 acggcctcgc gacgtcgctg atcgccacga cgaacggcat cgttcgcgtg cttcagatcc
    20641 aaggcccgaa gctcttcgtg ggcggggagt tcaactcgtt ccgctcggag gagcggcgat
    20701 acctggcggc tctcgacacg ggcacgggtc tgacgttgcc gtggaatccg gcagcgaaca
    20761 atcccgtgca cgcgatcgcg gtgaatggag cctccgtgtg cgtcggcgga ctcttcacga
    20821 cgctcggggg ccagatccga aatcgcctgg ccgcggtcga cgccggcacc ggagccttgc
    20881 tgccgtggaa tcccgacagc gactcggagg tctacgctct tgtcgtgagc ggaggcacgg
    20941 tctacgtcgg aggctacttc gcatcgatcg gcggtcagct ccgaagcttc atcgcggccg
    21001 tcgatgcggt caccggcgtg ccgaccgcgt gggacccgaa ctcgaacggg cagattctca
    21061 gcctggccgt cgcgaacggc gtggtctacg tcggcggcag ctacacgtcg atcggaggcg
    21121 ccgcgcgcaa ttccctggcg gcgctcgacc ccgccacggg cctcgccacg gcctggaatc
    21181 ccggcgccaa cgattgggtg atgtcgctcc ttccgggaac gggcgtcatc tatgccggcg
    21241 gttatttcac gtcgatcgga gggcagtctc gctcctacat cgcggccctg agcacctcga
    21301 ccggcctggc gacgagctgg aatccagcgg caagcgccca tgtggaagcg ctcgccatgg
    21361 gcacctcggt cgtctacgcc gggggagcct tcacgacgat cggaggacag ccgcggaacc
    21421 ggctggccgc ggtcgcgacg gggacgggac tcgccacctc ctggaatccc aatgcggacg
    21481 acatcgtcta ctccatggcc gtcaacggga gcacgatgta cgtgggcggg agcttcacga
    21541 ccatgggagt gtccgggcga agccgcgtgg cggccgtcga tatcgcgagc ggtgccacga
    21601 cctcgtggaa tccctcggcg gacggcccgg tgtactcgat cgccgccacg ggcagccggg
    21661 tctacctggg cggtgagttc ctcgaagtcg gaggactgcc gcagagcggc gtcgcgagca
    21721 tcttcccgaa catcaccgcc gtcatcgaag ccccgagcgc cgtcgagacc gtgctcggtc
    21781 cgccttcacc gaacccgttc gtccaatcga cctcgattcg gctctcgctc cagaaggatg
    21841 cggtcgtcac actccgcgtc ttcgacgtcc acggccggca ggtcgccgtg ctggccgacg
    21901 gcagctaccc cgctgggacg catgtgtttg ggtgggacgg gaggacccgg gccggccagg
    21961 ctgcgatgag cggcgtctac ttctgcgagc tcaggaccgc gaccgcttcc ctcaagcgaa
    22021 agctcgtcct cgttcgctag cgcggctcaa ggcgttcgta agattgtcga gtccgcgatc
    22081 gcgacgagcc tgtggatgcg ccaggacttg tcggacgcct gctccgccag cagcgtgtac
    22141 cgccccgact ccaccttcgg tttcccctcc tccgtgaatt cctcctggaa cgtcccgctc
    22201 tgcgccgcga gcccgcccac gaccttcatg tcctccggca ccacgcggaa attcgtcacg
    22261 tcgacgctcg cgtagaggga cgagaggaac tcctgaacgg cctggcggcc gttggcggtg
    22321 gccgcgttcg agtaaacgac cgaggcgtcc tcggtgaaga tgctctcgaa caggatcggg
    22381 tcgcgccggt ccgccgcttc ggcgtagtgg atccagagcg tgtcgatggc ggccttggcg
    22441 ttggccgcct gggaggcggt atcgaggtcg gatgtggcgg agcacgaagc cagggcgacg
    22501 agcagagcaa ccaggccgag cagggtccgc atagctcctc cgtggagacg tcgggtcgag
    22561 cggctcccag gataacaaaa gggcccggcg cgtgttgcgc gccgggcccg gtagggaccc
    22621 ccacgtcccc ttacccccca ctgcgtacgg cgtcccgtcg ctccgaccgc ctgcacgcag
    22681 gcgctttcgc tcggggacgc gcgtcggcct gacccccgtg caggcacgac gtcgaaatcg
    22741 gcgaaggggg gacgagagcg ttgctcaccg atgagcgccc ccacggcgtc cacgtcggtc
    22801 cggccgctca ccggcagcgc accggatcgt cccccctacc cgacttcgat cggttgcggt
    22861 ctcatcgacg cccgcccgtc ttggcaccag ccgtgccagc ctccctcgac tgtgccgcgc
    22921 gcggcaaacg caaggcgtcc acggtctgaa accgcgtagc aatacccatt cctgatcgcc
    22981 cgaacgtgga acattccaca aaggggatgg agggaattgc cggacttgta tccaggaaaa
    23041 cgtcggccga ggggtcgaac gaacagaaaa tgtcgcgagg ttggccgcct ctagcgagcc
    23101 gaggagcgga acgcctcttc gaccatgtcg gcgtggatcg cgaccgtgtc ctcgacgcgt
    23161 tgcgcgtagc ccccggcgag ataggccgcg accggaacgg agcgctcgcg cgccgcgtcg
    23221 aagacgagct tgtcccgcgc gcgcaggtcc tcgcgcgtca gcttgagccc accgagctgg
    23281 tcctcctcga acgggtccgc gcccgccaca taggccacca ggtcgggacg gtgactctcg
    23341 aggatgcgcg gcacccagtg tccgagcgca gccaggtaga ccttgccgtc tgccccccgg
    23401 tctagaccga cgtcctgatc gctcggtggc ttcacgagcg ggtagttgtc ctcctcgtgg
    23461 atcgagaagg tgaagacact gggatcgccc cggaagatgg cggccgtccc gtttccctga
    23521 tggacgtcca cgtcgatcac ggccgcgcgc tggatcgtcc catcgtggag cagatcgcgg
    23581 atcgcgatcg cgacgtcgtt cagcacgcaa aagccctcgc cgtgatccgc gaacgcgtgg
    23641 tggaagcctc ccccgaagtt cacggccgcg ccgcgctccc ttgccaggcg cgccgcgaga
    23701 atgctccctc ccgcacagag aaacgaggcg tccacgagct tcttcgagta gggaagctcg
    23761 agaatggcct cctcgagctc ggtgagcgtc ccccgcacga gcttctcgat gtactcctcg
    23821 tcgtgaaccc gggcgacctg cgcccgcgtg gcgggaacgg gctccgcgaa atcggtctcg
    23881 gatgcgagcc cgcggcgaac gacctcgtcc cggagcatcc ggaatttccg catgggaaag
    23941 acgtggagcc cgatctcggc gaagtacgcg tggctgtgga cgagcccagg cttgggcacg
    24001 ggtcctactc gaactggaac ggggagctga cgcggtgcgc ttccggcacg gccaccgcgg
    24061 ctccggccgg gacggcggag ccggacgcgg cggggacgag cgcgcgatac aggaccacga
    24121 gcgaggccat cacgaatggg aagaggaagg ccgagacggc cgacgtccag accacggtcg
    24181 cgaccttgag gagcgggtgg tcccctcctg ccactgccgt cacaacctga aggatctcgg
    24241 cggggaagga aagaaccagg gccgcgagga cgacgaaaaa gagcaccacg atcagccgtc
    24301 ccaggatgcc gccgaagtgc gacgcgacga ggctgaagct cacgcgaatc gaccggaacg
    24361 gtccgcgccg gtcgagcacg agcgccggaa acgcgaacca aagcatgaag agcatcagga
    24421 tggcgtagat gagacacggc accatcgcga ggagcccgat cgctctcgtc agctcttccg
    24481 gccgcgttgg aatcgtgcgg acgagaatgg cagccccggc gaaaaggagg aacggaggtc
    24541 cggccacgat gacgagctgc gcgaccgcgc acacgaggaa cgcgagcatc cgctcgagca
    24601 ccgagcggac ggagctcccc agcgtggtcg cgaccccgcg caaaattccg tcctggagca
    24661 ggaggaatgg catgtagatc agcacgagga cggggatgta ggcgggaatc tggacgagcg
    24721 ccgcgctcgt gaccgcctga ctgctcgcct cgtcgatcga ctgcacccgg tccatggaga
    24781 gcgcgacgta gaggcccatc ccgaggcggt agaagtagag aaagatgagc gccgggaatc
    24841 cgggcttgat ggatgccttg cccagcgcga ggacggtgcg cagataggtg gagacgggcg
    24901 gaagcgaagg acggctcgcc ggccggggca gcgtcacctg atcgtccatc gctgcatcat
    24961 acccggtcgg gccgagacgc ccaaggggcg cttcggttag gatgccgccg atgatccgag
    25021 gcatgtcgcg agccgcggtc aagatcaacg agtggacgga gcgctgggtc cccggcgcgt
    25081 tcaccatcgc gtggatcctc acgctcatcg tgatcgtcct cgcgctcatc ctggccagag
    25141 cctcgttcct ggattgcgcg cgcgcctggg gcgacggctt ctgggagctc ctcccgttct
    25201 ccatgcagat gtgcctcgtg atgttcacgg ggtcgatcgt cgccgtgtct ccccccgtcg
    25261 cgcgtcttct cgcgcggctc gccgcgctcc cgaagagccc gcgccagtcg atcctcctca
    25321 ccgcgctcgt ctcgatgctc ctggcgctcg ttcactgggg gctcagcatc gtgggaagcg
    25381 ccgtgctcgt gcgcgagatc ggccgcggcc ggacgcgcgt ggactaccgg ctcctcgtcg
    25441 cggccgccta tctcggcatg gggacgacct ggcacgcggg gctctccgcc tccgcgccgc
    25501 tcctcgtggc cacgccaggg cacttcctcg agaaggagat cgggctcatt ccggtgggtc
    25561 agacgatctt ctcgcccttc aacctcgtgc tcgtggccgt cgtgatcgcc gtgttcaccg
    25621 tgatcacgcc gctcctccat ccgtcgccgg agcgcaccgt gaccgtgggg gaggcgcagc
    25681 tcgccgcaat ccggagcttc gcgcctccgc gcaagccggc cggtcccctc gcgccggcag
    25741 cgcggctcga gtactcgccg ctcgtcaact ggaccttcgg agcgctcgcg ctggtgtggc
    25801 tgggagccta cctggccaag ccgcagcacc cgatcacgct caacgtcttg aatttcgtga
    25861 tgctcgggct cgcggtgctc ctccatccgt cggcggcgtc gctcgtggcc gcggccgagg
    25921 aggcgggagc gctcgtgttc gggatcatcc tccagttccc gctctacgcc ggcatgtacg
    25981 ggatcatcca gtccaccaag ctcgccgaga tcctcgcgga cggcttcgtc cgcatcgcga
    26041 acggatcgac gtacccgctc ctcgtctact ggtactcggg aatcgtgaac tacttcgttc
    26101 cctcgggcgg ctcgaagtgg gcgatcgagg cgccgtacat cctcgccgcg gggaagagcc
    26161 tcggcgtccc ggccgcgaag gtggtgaccg cgtacgcgtg gggggacatg atgaccgaca
    26221 tcatccagcc cttctgggcg atcccgctcc tcgccgcggc caagctcgac ttccgggaga
    26281 tcctcggctt ctgcatggtg cttttctgcg tctacgcgcc catcgtgtcg ctcgccttcc
    26341 tcttcttccc gcggtagact tcgggcgcgc acagcgcccg ccatgaccac ccagaccacg
    26401 cttccgctct tcgaggagat ccagctcgac cccgagcagc gcgccgccgt cgagcacggc
    26461 gacggtccgc tcctgatcgt ggccggcgcg ggcacgggaa agaccgccgt catcgcccgc
    26521 cggatcgcgc atctcatcgc gaccaagcgc gcccgtccgt cgcagatcct cgcgctcgcg
    26581 ttcaacgaga aggccgcggc cgagatgcag gagcgggtcg acgtgctcgt cccctacggc
    26641 tacgcggacg tcacgatccg caccttccac tcctttggcg aggacgtcct gtccgggcat
    26701 ggcatggaga tcgggatcgc ccccggattc tccgtgctcg accagacggc gcaggcgctc
    26761 ttcctcgcgg agcggctcga cgagctgggg ctcgcgcgct acgcgccgct ctccgacccc
    26821 acgcgctatc tctcgtccct cgccacgtac ttcggccggg ccaaggacga gccgatctgg
    26881 cccgaagggc tcagggaccg cgcggcgtgg ctcgacgagg aggactcacg cgaccgtgtc
    26941 ctcgagctgg cccacgcgta cgagcgctac aaccagctcc tctgggaagc cggcttcgtc
    27001 gatttcggcg acctcctcgc gctcacgctc aagctcttcg acgagagccc gggcacgctc
    27061 cgccgcttcc aggagcgctt cacgcatgtg ctcgtcgacg agttccagga cacgaatccc
    27121 gtccagttcg agatcgtgaa gcgcctcacg gcgctccacc ggaacctcgt ggtcgtcggg
    27181 gacgacgacc agtcgatcta cggattccgg ggcgcgcact tgaagaacat cctcgacttc
    27241 cacgaccagt acccggacgc gaagggaatc gttttgcgcc ggaactaccg gagcacgaag
    27301 ccgatcctcg aggtgtcccg gcggctcatc gtcacgaaca gggagcgcct cgagacgcgc
    27361 atcgggatct cgaagctcct gacgacggat gtcgaaggcg agttcccccg ctggcgcgag
    27421 ttcgcgaccg aagcggagga ggccgagtgg atcgcggagc agatggcgga ggccgtggct
    27481 tcggggcgcc ggcgctaccg ggatttcgcc atcctcgtcc gcaccaaccg cacggccgag
    27541 cccttcctgc gcgcgctcga ccagcgcggc attccctact acttctcggg cagccgcggc
    27601 ctcttccagc ggcccgaagt gaaggagctg gtcgcgctcc tccgctcgct cttccaggaa
    27661 tcgaggccgg aatacctcta cctgctcgcg gccgagggct acggcgtcgc cgaggacgac
    27721 ctcgcgcgcc tcatcgaccg gctcccgatg gagccgctct ccttcaagtc cgtgctcgag
    27781 cgcgccgccc aagggaccgg tatcttccgg gatctcgtct cggacgacgc acgcgggaag
    27841 ctgggcgaga tgctcgccca cctgagcgag ctccacgact tcgcgaaggg caagcggacg
    27901 ggcgaggtgc tctaccgcta cctcgagctc cgcggcgtgc tccggaagct cgtctcgtcc
    27961 gagcggttcg aggacgaggc gcgcgcgcgg aacatcgtga agttcttctc catcgtgcgc
    28021 tcgttcgaga aggtcgccgt ctccgatcgc gtcgcgctct tcctccggca cctggacgcg
    28081 atccaggact acggcgagga tcccgcggtc gccgacatcg acagcgcact cgacgtggtt
    28141 caggtcttga cggtgcacaa ggcgaagggg ctcgagttcc cggtcgtctt cctggtgcag
    28201 ctcgccgcgg gcaagttccc gacgaacaac cgaagcggcg gcctcgatct tccggcgccg
    28261 cgcggcgacg ccgcggctga ctccgaaacg caccgcgagg aagagcgccg cctttgctac
    28321 gtcgccttca cgcgcgcgcg ggaggagctg gtcctgacct tcgcgcggga ctacggcggt
    28381 ttcgggcgcg agcggcagcc gagcctgttc ctgcaggagg cgttcgatct cggcaagccc
    28441 ctgccgccga agcggcggaa gcgggcgatc gaggagctgg aggagcatcg gccgggactg
    28501 ccggcgctcg cgctcgtgga cgcgcacgcc acgcgggatc ctctggagct cagcgtgcgg
    28561 cgtctcgagg actacgagac gtgtccgctc aagtaccgct tcctccacga gatctccgtc
    28621 gaccccattc tcacgcgcga tcaccgcgtg aacttcggga acgcgatgca ccaggcggtc
    28681 gcccatgcgc tgaatcagcg gatgctgggg cgcgcggcgc cgttcgacga agtggccggg
    28741 gtgttccagc gggcatggcg gcgcgagggc tatcgctccg aggagcacga gcgccgccgg
    28801 ttcgagcagg ggctggacgc gatccgcgat ttcgtggcgc gggagatcga cgcggggccc
    28861 gtgccgtccg cggtggagcg gcacttccgc gtgaagctcg atgacgtcat cgtcacgggg
    28921 aggatggacc gggtcgacga ccaggacggg gagatcgtgc tgatcgacta caagacgacc
    28981 gatctggacg atccggagaa gggagacgcg gaggcgaagg acagtcttca gctctccgtc
    29041 tacgcgctcg cgtatcgcga gctgacgggg cgcacgcccc agcgggtcga gctgcgatac
    29101 gtgctcacgg gagacgtggg gcgggccggg cccgacgaca agcgcctcga aaagacgcgc
    29161 gcgaagatcg ccgccatcgc ggcgtcgatc cgtgcgggcg atttcgaggc gcgtccgagc
    29221 gagcggaact gctcgatctg cgcgtgccgg ccgatctgca gccggtcggt gcgctaggcc
    29281 cgcgcgcgac gagacgcggc gggccgtgcc gcggctgcgc taggccgcga ggacgttgtc
    29341 gaggatcgac gtgatctggt gcttgggcac cgcgccgacg atctgctcga cgatcttccc
    29401 acccttgaag atgtagaggc tcggaatgct cgtgatgcga aactggctcg cgacgcgttg
    29461 attggcgtcc acgtccacct tggcgacctt gaccttcccc agatacagct tcgccacttc
    29521 ctccaccacc ggagccacca tgcggcaggg gccgcaccat tcggcccaga aatccacgag
    29581 aacggggatc tgcgactgga ggacctccga atcgaaggtc ttgtcactca cctggagtac
    29641 cgcgtcggac atcgctcagg ctccttacca tgatgtggaa cacgttgggt cgccgtctgt
    29701 tacgtaagac cgaaaggcta acgtcttaga tgcccgggat caaggccgga ttcgaggggt
    29761 cctacgcggg cgggcgctcg ttgacactcc cgccccgggt tcctagactc acccgcttgc
    29821 catcccaccc agacttggag accccttgat cgaagtccag aacctcgtca agacgttcca
    29881 cgaccggaag aagggcgaga ttccggcggt gaagggcgtg tcgttcacgt gcccctcggg
    29941 caagatcttc ggacttctgg gccgaaacgg cgcgggcaag acgacgacgc tgcgcatgct
    30001 cgcgacgatc ctccagccga cgtcgggcac ggcgcgcatc aacgggcacg acatccatca
    30061 gcgccccgcc gaggtgcggc gctcgatcgg ctatctgtcg ggcgacacga agctctacga
    30121 ccgtctcacc ggccgcgatc tccttcagta cttcggggct ctcgccggga tgcacggcga
    30181 ccggatcgcc aagcggatcc aggagcttcg tgagaccttc cagctcgatg agctcctcga
    30241 caagcgcgtg ggcaagatgt ccaccggcat gaagcagcgg ctctcgatcg cgcgcgtcct
    30301 cttccacgag ccacaggtac tgatcctgga cgagcccacg gcgggcctcg acacggtcgc
    30361 ggcgcgccga ctgatcaacc tgatcggcga catggggaag cgcgggctca cggtcatctt
    30421 ctcgacccac atcatgaccg aggccgagcg gatctgcgac gagatcgcga tcatcgaccg
    30481 gggggagatc ctggcccagg ggacggtcga cgcgctctgt caccggaacg gatcggggac
    30541 cggcgagtcg ctcgaggacg tcttcatcca tgcggtggag gaggtgtccg ttgagcgcta
    30601 agaccttcgc ggtgttccgg aaggagatca cggacaccct gcgcgacggt cgctcggtct
    30661 tcgccatctt catcttcccg ttcatcctct acccggcgat gctcttcttc tcgagctgga
    30721 tccagtccaa gaacatggag gcggcgaagg agcttcaggt ccgggtcggg gtcgtgggct
    30781 cggaggcgct tccgttcgtc gtcgattcgc tggccgcgat ccagggcgtc acgccggtgc
    30841 cgatgaccgc ggtcccgtcc gacatcgagg gctcgggcgt gaacgccgtg ctcgtgatcc
    30901 cgcacgacct ctcggcagcg ctcgcgcgcg gggacagcac caaggtcgag ctcctctaca
    30961 aggacgcgga gaacaagtcc tcggccgcgg agcggcgggt ccggcccttc ctcgaccggc
    31021 tggatctcat gctgacgctc gactgggcga gggcgcaggg cgcgcacgcc gaagcggggc
    31081 ccgccttcac ggtggaaagc cgcgacacat cgtccaagac cgaggtcggc cgatacatcg
    31141 cggcgctcgt gatcccgtac atcctcatct tcatgatcac ggcgggagcg atgcacaccg
    31201 cggtcgacgc gacgacgggg gagaaggaga ggagcacgct cgagaccctg ctcgcgacct
    31261 ccgccacgag ggccgagctc gtgctcggca aggtgatggc cgtgctcgcc gcggccctga
    31321 ccggagccat cacaggaatc acggggctct ggctcacgtt ttcggtggtc gcgaaggcgt
    31381 tcccgaccat ggagtcgagg acgctggaca tcgcgatcag ccccgacaag ctcgtgatga
    31441 tcttcctcac gctcatcccg gtcgcggtct tcctgagcgc gaccctggtc gcgatcggct
    31501 gcttcgcgcg gagcatgcgc gagggccaga cctacgcgac gtacgtctac atggcggccg
    31561 tcttcctggg gctggggagc ttcggggagc agacgcctcc cttgaagaac ttcatgatcc
    31621 cgatcttgaa caccgcgctc ctccagcgcg agatcttgac cggcaccgtg caggcggcgc
    31681 atgcgaccgc ggccatcggc gtgaccgtcg gtgccgccat catcatgctg gccatggcgg
    31741 tccgtttgtt ttccaatgaa ggggtgctgt tccggaactg agaccacgcg ggtccgtgag
    31801 cccgccggga ggtggcatga agcgcgacgt cgacaccctg cttcgagagc gcggtctttc
    31861 cgccgcgctc atcatcaagg gggagatccc caacttcacc ttccgctatc tcgtcgggcc
    31921 gcccgcggcg catctctcga cgggcatcct cgtctggaag ccggggcaga cgccgcacct
    31981 cgtgcacggc ctgatggagc gcgactcggc cgccgcgacc ggattcgagc ggagcgactt
    32041 cggatcccac cgcttccgcc agatgctgga ggacgaggga tcgcgcgacg gcgcgatggc
    32101 gcgcctcctt gcgaaggtcc tgagcgacat ggaggtccgc gggccggtgc tcgtgcacgg
    32161 cacggccgat gtcgggcgct actaccacgt gctgaaccgg ctccgctcga tggcgccgca
    32221 gatcgagatc gcgcaggacg aggatctcgg cctcttcgag ctggcccgcg ccacgaagga
    32281 tcccgaggag atcgaggcgg tgcgcggcgt cgcggccgtg tgcagtcgcg cctacgagcg
    32341 catccgcgcg gtcatcggcg gaggcagact cgaagggaag aagctcaagg acaaggaagg
    32401 ctgggtcacc gtcggccgcc ttcgccgcga ggtgcggcgg gtgttcttcg aggcgaacct
    32461 cgaggagccg cacggcaaca tcatcgcgat ggggcgtgac gcgggcgtgc cgcacaacga
    32521 agggaacgac gcggacgtgc tggaggaagg gcgcccgatc gtgatcgacc tctatcccgc
    32581 gcagtccggc ggcggctact acttcgacgt gacgcgaacc ttctgcgtcg ggcgcgcgcc
    32641 ggccgagctc aaggagatgc acggtgtcgt gcgcgaagcg gtcgagcgca cgatggactc
    32701 gctctcgtcc gaggctccgg cgcggatgta tcaggagaag gtctgtgatt tcttcgaatc
    32761 gaagggacac aagaccattc gccaggacga gcgactggag gagggttaca tccacggcct
    32821 cggccacggg atcggtctcg aggtccacga gcggcccaat ctcggcggca cgtcgcagaa
    32881 cagggaccgc ctctcgccgg gctccctctt cacggtcgag cctggcctct actacccgtc
    32941 gcgtcagatc ggaattcgga tcgaggacgt ggtctacgcc cgcccggacg ggacgttcga
    33001 aaacttgacg ccggtgccgt acgacctgga ggtcgccccc gcgtcgtctt gatctccctc
    33061 agcttcccgc gcgcacgagg aatcgaatga gcccgcccgc cgcaaggcgg cgcgggaagg
    33121 agcgcgcgat tctcgcgggg cgggctccgt cgcgcgtcga ccacgacggc gacacgctga
    33181 ccgagctccg cctcctcgcg gagacagcgg gggccttggt cctcggcgag gtgctccagc
    33241 ggcgcggctc cgtgcgctcc tccacgttcc tgagcaaggg gaagatcgag gagatccgca
    33301 cgctcggaga cgagcagaac gccaacctcc tcctcctcga cgacgacctc tcgcccgccc
    33361 agaaccggaa tctcgaggag gacttgaagc tccgcgtcgt ggaccggagc gggctcatcc
    33421 tcgacatctt cgcccgccgc gcgcgcacgc gcgaggcccg cctccaggtc gagctcgcgc
    33481 agctcgagta catgctcccg cgcctgacgc gcatgtggga gcacctcggt cgtctcgggg
    33541 gcggcatcgg gacgcgaggc cccggcgaga cccagctcga ggtggaccgc cggcgcatcc
    33601 gcgagcggat cgccaagctg aagcgcgagc tcgaaggcgt cgtgcgcgag cggcgcgtgc
    33661 agcgacgcgg acgccggagc tgccacaagg tctcgctcgt cggctacacg aacgcgggga
    33721 agtcgacgct cttcaacgcg ctcacgcgcg ccaaggtcta cgtcgaggac cagctcttcg
    33781 cgacgctcga tcccacgacg cgggtcttcg ccgcgaagca ggggacgcag gcccttctca
    33841 cggacacggt gggattcatc cggaagctcc cgtcgcacct cgtcgcgtcc ttccgcgcga
    33901 ccttggaaga ggtgaccgaa gccgatctcc tgctccacgt ggtggacgcg tcggagcccg
    33961 atcccgagcg ccacatcgag gcggtacaca ccgtcctcga ggagatcggc gcgctccacc
    34021 gcccgcgcct tctcgttttc aacaaggtcg atgcgcttcc ggacgaggtg gcgtggctcg
    34081 gccttcgcgc gcggtatccg ggcgcttcgt tcgtctcggc cgtgacgcgc gagggcattc
    34141 ccgagctgcg tgacgcgact ctcgcgcggc tcgcggagga gagccggccc gcgcggcgtg
    34201 tgaacgggga ggagccgagg gcgtgaccgt cgcgggagtg ggcgccgcgg tgggcgccga
    34261 gcgcgtccgg cggatccagg aggcgcttcg cgaggacggc gtcccggcgt ggctcctctt
    34321 cgatttccac ggctccaatc ccatttcgcg caaggtcgcc ggcatggtgt ccggtcccga
    34381 cggtccgaag acgacgcgcc gatggttcta cctcgtcccc gcgcaaggcg agccggtcaa
    34441 gatcgtgcac cggatcgagc cttcatcgct cgatcatctc ccgggcgaag cgctggtcta
    34501 tctccgctgg caggagctaa gggacgcgct ggccaaggcg gtcgccagcg ttccgggccc
    34561 ggatcgcgtc gtcgccatgg agtactcgcc ggacgccgcg ctcccgtatc tctcgcgcgt
    34621 cgacgcgggc acggtcgaga tggtgcgcgc ggcgggggcg ggcgtcgtct cctcggccga
    34681 cctcgcgcag cgcttcgacg gcacgctcgc gccggaggcg agggaggatc acaagcgcac
    34741 cggcctcgtc ctgaacggga tcatggccgc cgcgttcgcg agggtgcggg accgcgtgcg
    34801 cgccggcgcg cctctcaccg aggtatcgct ccaggcgtgg atcctcgagc aattccaggc
    34861 cgaggggctc gtggccgacg atccccccgc ggtcgcggtc aacgaccacg cgggcgatcc
    34921 ccatttcacg acgcgggccg agcacgaccg gccgctccgg cggggcgatc tcctcatgat
    34981 cgacgcctgg tgcaggcggc ccgcgccggg gtcggtgtac gccgactaca cgcaggtcgc
    35041 gatggtcgac tccacggtgc ccgagaaaca ggcggagatc ttcgccgtgg tgcgggacgc
    35101 acgcgacgcg gctatcgaga cggtgcgccg cgcgcttcgc gcggacactt ccatccgcgg
    35161 atgcgatgtg gacgacgccg cgcgcggcgt gatccaggcc cgcgggtatg gcgaccgctt
    35221 cctccaccgc acgggccatt cgatcggcga ggaggtgcac gcgaacggcg tccatctcga
    35281 caatctcgag acgagggacg agcgtcgcct catcgacggc acgctcgtgt cggtggagcc
    35341 cggcatctac ctccccgaat tcggcatccg gagcgaggtc aacctcctca tcgatcgcgg
    35401 cgacgcgatc gtcaccgccg agccctacca gcgggagctc cccgcgctcc tcgcctagtg
    35461 gccgcacgcg acgtaccgga agccgagatc cggtttcgca cctcgcgcgc cggcggtccc
    35521 ggcggtcaga acgtgaacaa ggtggaaacg cgcgtcgagg ctctgtggaa tctcgacgcg
    35581 tcgcttacgt acaccgacgc gcagaaggcc cgcatccgaa gcgagctcca aacccgggtc
    35641 ggcgcggacg gcacgattcg cgtcgtgagt cagcgccacc ggagccagtc gcgaaatcgc
    35701 gacgccgcag tcgagcgtct ccgcgcgctg gtggctgggg cgctcaggcc gaggcgcaag
    35761 aggaaggcga cccgccccac aaaggcctcg cgcgaatccc gaatggagtc gaagcgccgg
    35821 cgcgccgaga cgaagcggga ccgaaggtcg atccccgagg actgagcgcg aggaccaccc
    35881 actcaacacg ttgtacctag ggcacttaag agcctatccg cccgcctccg cccgcccctt
    35941 cgacccagaa atcccctcaa caagacctcc tcccaagtcg atatgtagca agagaaatcg
    36001 cggtcgcatg cccggctcgc gccggtccat gcccgagaca cctggaggcc gtcccccatg
    36061 aagtcgtttc cgacgcaggc aggtccgcgc gcgatcgtcg gcgcggcgct cgtgatcctc
    36121 gccctcgccg tggccgtccc gatcgtgtgg aagcgtccca cgagcgaacc cggcgtctcg
    36181 tcgggtgaag gatcgagcga gccggcaccg cccgtgctcg gcccgccgtc cggtccgcgg
    36241 gtcttccgct tctatcagga atcccccacg acgctcgatc ccgcgctcgc cgcagacgcg
    36301 tactcctcga cggtggtggc gcagatctac agcccgctcg tagggctcac gtcgaatctc
    36361 gagcccacgc cgcaggtcgc ggattcctgg accatctccc gcgacgggaa gacctacgtc
    36421 ttccacatcc gcgaaggcgt gcgctttcac aacggccgcg aggtcacggc cggggacttc
    36481 gtgtacacgc tgacccgctg cttccaggag ccgttccgct cggaggggct cgcggcgggc
    36541 tacctcgacg ccatcgacgg cgtgcccgag ttcctcgccg gcaaggcgac cagcatccgc
    36601 ggcatccacg cgctcgacga gaagcgcctc gagatcaagc tgagccgccc gtaccgtccg
    36661 ctcctctcgg cgctggcgct cgaccagacc tcggtcgtgc cgcgcgagct catggaccgg
    36721 cacgggaacg cgatcctcga ggtgaagccg accggctgcg ggccgttcaa gtacgtgcgc
    36781 cgcgacgatc ggagcgttgt cctcgccgcg aatcccgact atttcatggg agccccggcg
    36841 atcgattcgc tcgtgttctt cacgccggtc ggcgacgtga tctcgcaggg ggcgaacgcg
    36901 ctcctggacg ggcgcgccac gctgtcgacg ttgccgatcg accgggtgga ggagttccgg
    36961 gcccgggccg gcatcacgat gctccgctgg caggatctca cgctcgcctt tctcggcatg
    37021 aacgcgacga tcccgcccct cgacgatcgc cgcgtccgtc aggccatcgc gttcgcggtg
    37081 gaccgtcagg cgatgatcca cttccagccc gaggggaaga cgctcgccac cgggatcctc
    37141 gcgccgggcg tgccgggcta ttcgcccgcg acgcgcacgt atccatgcga cgtcgcgaag
    37201 gcgaaggctc tgctccacca ggcaggctac gggccggatc atcccctgcc cacgctgaag
    37261 ctctacaagt cggtcacgtc gaattcaggg gtccgggcgc tcgactcgct cctggtgcgc
    37321 tcgctcgccg aggccggaat caaggtcgag atccagtacg tcacctggaa ggtgctggac
    37381 gacgcgatta ccggacgcaa ggcgcagctc ttcagcctct cgtgggtcgc ggacattccc
    37441 gaccccgaca cgttcctccg cgcgctcttc tactcgtcga gctcgaccaa ctacttcaag
    37501 taccggaacg cgaccgtgga ctcgctcctc gacgccgccc ggagcgcgga agatccgaaa
    37561 gtggcccagg cggcctatca gaaggccgag gagcggattc tccgcgacgc gccgttcgtg
    37621 ccgctcttcc atcccacgaa cttcatcggg ctgcgcagca acgtcgtcgg tctccagatg
    37681 aacccgctcg gcatctcgac cctggccatg gagaagctcc gctttgcgga gccgaaggcg
    37741 gatggcgaca accgaagcgc gacccgctga cgctcctcgc cgctcgcgcg gcggtctgca
    37801 ggcgcgaatc ttcctcacgt tcggcgtcat cgtcgtgggg ctggtcacgg ccgcggtcac
    37861 gttctttgtc cggagcgagt ccgattcgct ccttcgcgag acgaagaagc gcggcttcgc
    37921 cgtggcccga agcatcgcct ggctctccac gccggctctc ctctcctaca actacatcgc
    37981 gctgaaccag gccgcgagcc gctcgcaggc agggagcgag atcgcgtacg tcgtcatcta
    38041 cgacaaagag ggccagatcg cggcggacag ccggtccccc aattccttcg gccagccccc
    38101 gcgggagacc gccgacttcg ccgcccagac cgcacgggag gagcggtgga gcttcatcga
    38161 gccgcggcgt cccggcgacg cgcgcattct cgagatcctc cttcccgtct acgtcgaggg
    38221 ttacgaggtg aagtggggca cggtccgcgt cgggatctcg ctcgaagggc tggacaacga
    38281 ggtggcggcg ctcacgaaga acctcatcat cgcgggcttc ctggcgtcga tcctgtgcct
    38341 cctcggcgcg cggttcgcgt cggggcgcat cacgaggccg atcggccggc tggtcggcgc
    38401 gacacgcgcc attgcgcagg gggactactc gcagcggctg aatctgaaga gcggcgacga
    38461 gatcgagacc ctggcgtggc acttcgacca catggccgac gaggtgcagc gccagcaggc
    38521 cgagatcctg gcgagccggg agaatctcgc ccgcatgaac cagaccctcg aggccgccgt
    38581 cgaggcgcgg acgcacgcgc tcaccgaatc ggaggccaag taccggatcc tcgtcgacag
    38641 ctcgccgcag ggtcttctga tcgtgcagtc cgggaaggcg gtcttcgcga acccgaccct
    38701 cgagccgatg accgggcgcc ccgcgtccgc tctcgtccgg cccgacttcg atccgatgca
    38761 gctcttcgcc ctcgagtcgc gggctctcgt gcgcgaggcg atccaatcgc ccgctcgcga
    38821 ccgggcgccg gtccaggccg agatggtcca tgcgtccggg cggaaggtct tcgccgaggt
    38881 ccaggtcgtg ccgctcgtct tccagaacgc gccggcctcg atgctcctcc tgtccgacgt
    38941 caccgcggtc cgcgatctcc aagaccggct cctccgcggc gagaagctcc gcgcgctcgg
    39001 tgagctggcg gccggggtgg cgcacgactt caacaacaac ctcgggatca tcctcggtcg
    39061 cacgcagctc cttctcatga agacgtccga cccggaggtc tcgtccggcc tcaacgtgat
    39121 ccgccaggcg gcgatggacg gcggcgaggc cgtgcgccgg atccagcagt tcagccgcgt
    39181 ccgcgaggac cgcgcgcacg aagttctgga gctgggcgag atcgtcgtgg aggtcgtgga
    39241 gatcacgcgc ggcaagtgga agaacgagag cgagcggcgc ggcgtcaagg tggacgtgac
    39301 ggtggaagcg tcgaatccga gccccatcct cggctcccgc gcggagattc gcgaggcgct
    39361 caccaacctc atcttcaact ccgtggatgc gctcccggag ggcgggacca tcaccatccg
    39421 ctgccgctcg gaccaaaagg agtcgatcgt cgaggtggcc gacacgggga tcgggatgag
    39481 cgaggacgtg aagagccgga tgttcgagcc cttcttcacg acgaagggca tgtccgggaa
    39541 cgggctcgga ctctcgatgg tctacggcat cgtatcccgc cacaagggga cggtcgaagt
    39601 cgagaccgcg cccggcgccg gcaccgtcgt tcgcatgcgc ctgccggcga ccgatctcga
    39661 gggaaccgtc atgcccgcgc gtccgcggta cgaggccccg gcgcgcgcac gcatcctcgt
    39721 tgtggacgac gagaccgaga tcctcgcggt gctgcgcgag gcgctcgagc gcgtggggca
    39781 caccgtcgaa accgcctcct cggggaggga aggcatcgag cgcttccgga acggagcctt
    39841 cgacgtcgtg ctctcggatc tcgggatggc ggacgtcggt ggatgggacg tggcgcgcgc
    39901 ggtgcgcgag aagggcccgc ggcgcgtggt cctggggctc gtcaccggat ggggagccac
    39961 gatcagcgag gagatggtca acgcccacgc ggtgaacttc gtgatcgcga agcctttcga
    40021 cgtggacgat ctcgtgtcga aggtgaatca gtccctggag gcggtcgcta tgcctccagc
    40081 cgcttgatcc agcgcacgtc ccccgacttg agccgccgct cccggtagcc caccgcttcg
    40141 aggaatcgct ccccgtcgtc gagccccatc gggacctccg ccgtgatcga ctgcgccgcg
    40201 tgctcctcgg cgaggaacgc ttccaccatc tcgagcgcgg cgcggccgta tccatggccc
    40261 cgctccgcct gcgagagcac gaggccgcgc agcacggacg gcacctcgtc ggagccgacc
    40321 tcgacttcga tcgtcccgat cagcgccttc gtgtccgtgc gccggatggc gaagtagtag
    40381 cggctgtcgt cggcgatcgc ctcttcatac atctcttcca cggtattcgg atcgaagtcc
    40441 gaggtgccgc tcaaggcctc gaagtagtct ggtgcgtcga ggtagacctt ccagatggtg
    40501 ggcagatgct ccggtcccgg cacctccagc gtgaccaggg gaccgtcgag acgggtggtg
    40561 gattcgctca tcgtaggaga acggcctttt gcgtgcgcac ccggtgcccg gaccgtagct
    40621 ggatgaagta gacgccggtc ccgaccggat tcccccggtc gtcggtgccg tcccacgtga
    40681 acggcgaagt acccgcgcgg ccggacaggt cagcgagggt gcgcacgagc gacccgtcgg
    40741 cacgatagac ccgtaccata aagcggccgt cctggtccag ggacaccggg aacgaagcgg
    40801 cgcgcgcgaa cggattgggc tgcacgcggc cgatcgcgaa cacgcgcggc gcgccgccag
    40861 ggcccacgcc tgccgtcacc gccgcgttca cggggcccgc cgtgctcgac ggctcaccgt
    40921 cgcgtgacac cgtgagccag tagtcgtagt ccacgcccgc ggtcacgtgt tcgtcgacat
    40981 aggtgccgct ccgggactcc caaggaagaa tcgcgactgt cttccggccg tcgtccgcgc
    41041 tcgcccgctc gatttgaacg gtcgagcccg tattctcgga cagattccat tcgagccgca
    41101 cgcccggccg atccaggagc gcgatcgcgt tgaagctgtt gatcgcgatc gccgtcgcgg
    41161 cgcagtcgtc cgtgccggcc acctgcacgt cgtcgatcca ccagcccaga ccttcgttcg
    41221 gggggacgga ccggaagtgg aacgcgaact ggacgggacc gctcagaccg tcgagcggga
    41281 agaccgcttc ggcgaacgtt gcctgcgtgc cggtccagcg gaagccgttc gagtagccgc
    41341 ccaccgggac gatcgtcgtc catgtctccc cagcgtcgac ggatacctcc acgttgaggc
    41401 cgtccacgcc gggctcggtc gagtaccgct gccagaagcg gagcgtgtcg gcgggaccgg
    41461 ccagccagac gggctggctc acgaggcgcg cgtcctcagc cggtgcgtat gtgtcgaggg
    41521 tgcccggctt tccgagacgc cacgcccacg tgcccttcgg cgtgtggttg atgccggcct
    41581 cgcgatgcca ctcgttcacg ccgtcgcagc cgagcggcac cgagatccag cggcggatcg
    41641 tgccctcgaa ggaatcgcag atccccgtct tcgcgccgac caggacctgc acgctgtcgc
    41701 tgtcgacgag gcccgcggac gcatggactc cgtgcgacac gctcacgccc cgcgggtccg
    41761 gagtgggaag gatcgtggcc gtgatgctgg agcccaggct ggtcgagctc gcggcgatcg
    41821 acccgtacgt gatcgtgtcc gtgtccacgc tcatgaaggc gggctcgggc cggatcttcg
    41881 cgccgaggga tccggtggcc gtggggccga tgttcaggag ggggaagttg agatgcacga
    41941 cctcgcccgg ctcggcgcgg ccgtttccgt tgttgggcgt gcccaaaagg ggagcgtcgt
    42001 cgacgacgat gggcagacgc cgcacgtcca cgccggtcac gaagaagctc gccatcacgt
    42061 cccctccggc ctcggcgatg ttccggatcg cgacctggct cggtacgtgg aagcaggagg
    42121 tcgcgctgct cggacacgag ttgccgttga acgcggtggt gccgagcggg cccggccacg
    42181 gatcgtagcg atcgcctcga ttgccgttca ccgaggtgga gacgccgcag acctccttcg
    42241 tgccgagcgc gcccgccggt aggtcgaggt tctggtttcc ggggtgccct ccctgctcgg
    42301 gccctcccgc ttcgaccacg tcgaccatct tgtgggcggg gtcgtcgttg ttggagcggt
    42361 tctcgtcgat gtggtagatg acgaggccgt gcgcggggcc gagtcccgcc tcgaccgagg
    42421 agcggacgag cgtggaatcg aagccgaccg gctgccggtt ctccacgagg aagtattcgt
    42481 tcgactcctg gccgttcgtc cacaagcgga agacctggcc cgagctttcg acgggcggga
    42541 tggtcacgcc cgatgagtcc tgcgtcaccc aggtcggctg gacccagccg agcctcgcct
    42601 tgcaccacgc cgacatgtgc gccggcgtcg tgccgggcgc atgcgcgggg gagtgcgtgt
    42661 agagcccgta cgccatcaga tcccactcgc cgtcgcccga gctctggttg tcgtccaggt
    42721 cgtagaggtc aggaagcccc aggatgtggc cgaactcgtg gcagaagacg ccgatcgaga
    42781 tgagatcgcc cgcgctgtag tacgccgagt tgagccccca ctcctcgggc tccgtcgtga
    42841 atgcgtagag cgtggtcccg tccagcgtga ctcccccggg gaggttccac ttgagcgacc
    42901 agatctggtc gacgctcccc gtctcctcgc cccctggccc cgcgtgcacg acgaacacgc
    42961 cgtccacgaa gccgtcgccg tcgccgtcga actgggacca gtcgatgtcg gggtccgcga
    43021 gaagcatcgc gtcctgcgcc atcccctgcg cgttcgtggg aaaggcgccg aatcccgact
    43081 ggctgttcac gtagtacgag taggggtggg gcgccgtgta ccagcgcgtc accacgccct
    43141 gcacgtcgag ctgattgcgc gagacttcgc gatagtagtc gcggaagctt ccggtcggac
    43201 gcgtgccgac gctgaagagg aggctgtcgt acgcctcggg cgggtgcgcc agcgtgtccg
    43261 ccgcgaggtc ggggaactgg agcaggacga cgaccactcg cttgatgccg gtcgtggtcg
    43321 cgcgcgcttc cgcgagatcc acctcgggag cgcgatgaag ctgtgagtag agcgcgcggg
    43381 cgcgggacac cgtgcgggcg cccggccctg tgttgtcgag aggaggtgcg gcgtgagcgg
    43441 cggtcgcgcc cagggccagg cacactccaa ggagcagccc ttgaaggcgc aaactcgaga
    43501 actccttccg gcggtgaggc ggttaagtta cgggtggggt tatagttcgg cgggagccgg
    43561 cttcgtgcgc ccgaagctcg agcgctcctg cttcccccag ccaccacgcc cgagaagcat
    43621 atcgacgagc gcgcccaagc ggaagaggag agtcaggata cggtaggaga agttttcgag
    43681 gatgccgatg gccaccatct gcgcgaagtc cccccaggac cggtagaggc cgaacgaaag
    43741 ctcctcgagg aacacggcgg aaatggacag gaacgcgccc gacgtgatgg cgaggaagag
    43801 gaaggcccag aagtagagcg gatcgagaaa gcccatgaag tacgcgatcg ggatgatgat
    43861 gtagcccacc agctccacga tcggtcccag aaactcgaac aggaagtagt acgggatgcc
    43921 gatccagcga agcgtcccgg agtgcttgcc ccagatcagg ctcttgtggc accagagcga
    43981 atggaccatc cctcggtgcc accggctccg ctgccgcgcg agcgcgccca acccctcggg
    44041 gacttcggtc cagcagatgg gatcgggcag aaagacgacc gagtactcgc ggttcatctc
    44101 ccgcatgcgg cggtgcagcc ggagcacgag ctccatgtcc tcggtgaccg tgctcgcgtc
    44161 gtagcccccg gcctcgatca ccacatcctt ccggaacaga ctgaacacgc ccgagagaat
    44221 gaggaggctg ttcagggcgg acagcccgag ccggctcgcc acgaacgcgc gaaggtattc
    44281 gaccacctgg aacgcggcga ggatgttcgt ggggagcttg ggctcgacca cgcggccgcg
    44341 ctggatcttg cagccgttcg caacgcgcac ctggccaccg accgcgatca cgcgatccgg
    44401 atcctcgagg aacgggcgca cgacgcgcag gagcgcgttc tcctggaaga ccgagtccgc
    44461 gtcgaccgtg cagaagagcg gataccggga caggttgagc cccatgttga gcgcgtcgga
    44521 ctttccgccg tgctccttgt ccacgaccac gaggttgggc accgcggacg agcgatagat
    44581 cccgccgatc gcgcccaggg tcgtaagcct ccgccggtag acggcctcgc ggcgctcgag
    44641 cccgaactcc cggaccagct tctggagcgt gtcgtccgtc gacccgtcat tgatcacgat
    44701 cacctcgtgc tgcgggtaat cgagcgacag caccgagcgc accgagtcga cgatgccggc
    44761 cgattcgttg tacgcgggca cgagcacgct gatcgggaag gtgaagtgcg acttcgcgac
    44821 cgcctcgtag tccgcgtaga agttctgcgc gaggcggcgg cgcatgtcga agaaggagag
    44881 gatcaggagc ccgaacagga cgagctggag cgcgctgaag tagagcagga tcacgaggct
    44941 gatcgtgttg atcagatgca tcgcccaggg ctcgaggacg tagccgctca tcacgccgtc
    45001 tcccggtggg gcgtcggaac gatgcccatg gccgagagcc cctcggcggc gctgtcccgc
    45061 gcgaagcggt cctcgctcgt cagggtgcgg aggaggacgt ccttcgacgg ctcgcccagc
    45121 gagacgagcg cgtccagcgc gttcttcctg acccaccacg accggtcgcg aagaagctcg
    45181 ccgagcgcgt cgacataggc ggtgtcctgg atcttcccga gcgcctccgc cgcgtgcgcg
    45241 cgcacgaacc agatcgggtc gtggagcaga ggtgctaaga gcggcgccgt cgagcggtcg
    45301 cccacgtgcc ccagcgccca cgtcgcggcg gagcgcacgt cggaatcggt gtcgaggagc
    45361 gcgtccgcga gcgaccgcgt cgcgcgcgga tccttgatct cgccgaggat gcgggcagcc
    45421 cagaaccgcg ccttgggcga gccgtcgcgg agcgtcttga tgaggagcgg cgccgtgcgc
    45481 gagcccatct cgatgcagat ggccgcgatg cgcgcggaga cggcctgatc gtgcttgccc
    45541 aggagcgcga ccagggcttc ctccgtcgcc tggaggcgca tccgcccgag ggagcgcgcc
    45601 gcgacgttcc ggacgtcggg gtcccggtcc tcgagcgcgc tcaagagcgt cggcacggac
    45661 tgcggcagct tgagctcccc caggatccgc agcgcgtgcg cgcgcgtcca cttgagccgg
    45721 ctcttcgcgt ccttcaggtg ccggttcacc gcccccgact cctcgagcgt gcggagcacg
    45781 tcgaggcgaa gggacagctc gagctggctg atctcctcgg ccgcgagctc cgaccagcgc
    45841 cccgagagcg actggacgat ccaggggagc gcctcgcgct gcccttcggg cgaccggagg
    45901 aggaggaacc gcttcctcag attctcgagg aagcgctgct tcgaatcctc ttcccggttc
    45961 gagtagatct tgttgacgat ggccgcgatg gtggtgagcg cgacgatccc gaggaggagg
    46021 agcagcgtga agatgaggga ctggtagtcg atcatctcac caccgcgccg atcccgagac
    46081 cccgaggtac caggtctcgt aggaattgtt gtcgacggag taggaggcct cgaggccgag
    46141 cgggagcgtg ccgaacgcgg tcaggttcag atagccgctc caggtgtgcg tccagatgtc
    46201 cgcgaagttg gtggagttga ttccggcgaa ctcgtagcgg aatccgtagt cgatcgccga
    46261 gcgctcctcg tagcccgcga tggagactcc ggcggagcct cgaacgcttc cgagcggggt
    46321 gaagtagctc cggctcgtga agtcccagtc gcgataggtg agccggctgt ccaccgtgac
    46381 gctcggagtc cccgtcttcg cccggtagga cgcgccacct cccgccgtcc agcggcggtt
    46441 gtcgtcggag tagaccccgc gggagaccag ggcgtccgcc gtgccgcgcg cgctccactg
    46501 ccagccgacg ccgccctcgt actcgtccac gtgccccggc acgaacgcgg tgtcgggcgc
    46561 cgtctccttg agcgcgatgt agtcgcgatg aatggccccg cgcggcgtga agcgccccga
    46621 gaagcgccag acgcgcgcgc cccacccgaa gaacgtgtcg tcctcgccga gcgggaagag
    46681 catcgttccg gggttctgat cgaacgatcg atactcgacc gaagccgcgg cctgccacgg
    46741 ataccctcgg aggaacgagc cgtcgatgcg cgtcaccacg ctcgacaggt cgtagttctt
    46801 ggcgctgccg gcgcccggcg tttcgacgag atcgagccag gagacgcgtc ccgtgaacgt
    46861 ggctctgcgg gccatcgtgc cgcgcccctg gatgtagggg ccgtcggtct cgagcgtgaa
    46921 gtcgggcgcg agatcgacgt actcgcgctc gtgggacgcg ctccatcccg tgcccaggga
    46981 cggcctgagg gcggtcgcga ccgcgccctt ttcctcggcc atggcctccg ggtccgcatg
    47041 cagggactcg gcgcgcgtca cgaagcggtc cgacgcggtc gcggcgcccc gccagcgtgc
    47101 gaccgacgcg aggccgagcc acgcgtcgcc ggtgcgcgga tccgcggcga ggatgcgctg
    47161 gtactcggtg gacgcctcgg cgagatcgcc gctccaggag aggacgcgcg cgtagccgac
    47221 ccgcgtctcg agattcgtcg agtcggcctg gatcgcggtg cggaactcgc gcgccgcttc
    47281 cgtgtggcgt ccggaccacg cgagcacgcg cgcgtagctc gcgcgaagcg tggcgtcgtt
    47341 cggcttcttg gcgaggagct cccggtactc ggcgagcgac tcgtcgtagc gccgctgcca
    47401 cgagagcacg cgcgcgagga ggagccgcgc gtcgtcgttg ttcggacgca tgcggagctc
    47461 cgactggagg agcgggatcg cttcggcttg ccggtcttcg cgaaggcgcg cgcgcgcggc
    47521 atcgtaaggg ctcacgcccg ccgtctgggc gagggcgggc gcggtgtgcg cgaggatggc
    47581 gatgaggagc gccgcgcgga tcgccgcgcg gaacggccta cggccggtcg gcgtcgacct
    47641 tctggcttcg gctggcttca cgggcttcca ttccctcgaa ggcgcgctgg atgatgtcgg
    47701 cggaacgctt gacacgttct ttagttatcg gcgtttccca cgtcgtccat gagtaggacg
    47761 cctgaggcgg tgtcttgacc gtaagctctt cattcatagt gctttggatg gatccggccg
    47821 gacccaggac gaagacctcg cctggcttca cgggtttggc tccggccagg gtcggcccca
    47881 gcgaatcggg acggaagcag gcggccgtcg tggcccaggc gagaaggccg acgcccggcg
    47941 tctgggtcgt ctcgatgtcc gctgttccgt ccttgtcgtc cagcgcggcg cgggacgagg
    48001 ggtcgccgac aacaaggccc cacggaattg cgatctccac cgtggtcccc agcgaatcga
    48061 aggcgcactc gagccgcccc caatcgaagt acttcgccgg aaagaacaag ccgttccgcg
    48121 cgacgcgctc gcggttcgtc tccacgacga ggggaacgta cgcgcccgca gcgttcgcca
    48181 cgggtcgcgc cggcgcgcga cctcgccgcc gccagtcgcg tccgtcccat tccctcatga
    48241 aggacgagac gttcatcgcg cgatcctggt agagctccgc gcccccgcgc gggctgccct
    48301 tgctcggagg cgtgaccagg agcgcgagct cggcgccgcg ggaccacgag acgggaagcg
    48361 gctgcggaag gcgacggtcg ccgcgcgcgg gatcgagcac gtcgaacgag actccgaacg
    48421 tgcgcaccgc gggtttgcgc ggcgcgacgt cgagtcgcag atagagggag gtttgatcgg
    48481 atgtgacgta gagagcgcga agcggggctc cgccctggac ggcgtcggac gccgcgtaag
    48541 gcgaaatccc gctccagtcg tcgatcgcgc cgtcgatacg aatgggcgcg ggagggtcga
    48601 acgccaggag accgtagttc tcctcggggt cgagacggtt cagccagagc ggatcgcgat
    48661 cgcgtgggcg ctcgcttcgc tcgacgagcc agttcacctt gtaccactca tcgaagagcg
    48721 agaacagcag cgatcccgat gcgccggact cggcgatgtc ctcgaggagc cggacgtcgt
    48781 cctgtccctg ctggatctcg gtggcgccac cgtggtggat gccctgcggc tgctggtgtg
    48841 cgaccccgcg gctggtcggc acgccgtatt ccccgatcag gagcgggatc ccgcgcgtgt
    48901 gcgccttgag gtccgcgaga tagccggcgt acgagcagac gccgtggcgg tcgtggaacg
    48961 cctggtagtt cgggtcgagg ttcatgaagt cggggtagta gggatacgca tggtagttcg
    49021 cgaagtagcc gaggaagccc gacgtgagcg tggcggaatc gaccggcacg agatgcgatg
    49081 gatcgacgga gtaagcgtcc tcgtcgtgct ccgcttcgcc gccaccccgc tcggtctccg
    49141 tcgggtggcg catcggatcc agcgtgggcc agttcacgaa cgagaccgcg tgcgcgcgcg
    49201 cgtagtggac ccgttcgtag gacaccgtga gatcgcagac gcggccgagc cacgcttcca
    49261 tcgccgtgcc gtgctcgact ttgaaccgag tgcccgcgaa ttcggtgatg tccggatgga
    49321 gccgctgcgt ctcgcgcacg gcgtagggct cccattcgcg gccgatgagc caccccgcca
    49381 gccacgggga gacgtccgcc tcgtagcgcc ccgaggcgtg ccccgggcgc tcgtcgacga
    49441 tcgcgttccc gtgcacggcg tcgatcgcga gccggagctc gttctcgaat ccacgcgtgt
    49501 acgcttcgtc ccagaagtcg ttcccgtcgg gcagctcggt ccagacttcc tggaagagcc
    49561 agatgggatc gttcggatgc gcctcgttct cctcgcgcag cgcttcgtag aagcggggcg
    49621 gatggagcgc gtacacgcgg atcgagttcg cgtgcatcga gcgcgcgaac cggagccagc
    49681 ggcgatagtc ttccttcgtg atcgcgaatt cgccggggta atgccccggg ggcgcggcgc
    49741 ccaggttgag cccgcggaag aagacggttc gggagccgga gggcgtcgcg atctcgaatc
    49801 ggtccccgcg cgcgagcacc gcgcgtgtcg gtggggaggg aatgggcgcg cgtggatccc
    49861 ggatggtctt ccacgggcgc ggcccggcgt ctcccgcgag agcgggaagg gcgcagccca
    49921 ggatcaggac ggggaccagg atgtcgcgca gccgcatgga gattctcccg atggggtatt
    49981 cgggaacgat cggcagctca agccccgttc tgcagcggac gccgctccgc gcggctcacg
    50041 gaaagagcgc ccggagcgcg gcctcgtggt cctcgaagga gtcgaggagc gcgtccggat
    50101 cgcacgcggc cagcgcttca cggctgatcg agccggtcgc aaccgcgacg gcgcgcgcgc
    50161 cgatggcgcg ggcgcaggcg atgtccttcg gcgtgtcgcc gatgaccgtc gtctcggagc
    50221 cggagaaggc ccggccggag aggacgcgcg ccttctcgag cgcgatctgc gcgaggacgg
    50281 cgcgattctc gtgatcgtcg ccgaaggcgc cgaagcggaa gtacggctcg agattccagc
    50341 gccgaagctt gagccgggca ccctggtgca cgttgccggt caagaggccc acgagcacgc
    50401 tgtcgagctt gttgagccgc tcgagcagct cccggacgcc gggatagagg tgcgcgtcct
    50461 gatgctcgcg gacctccaca gcgagtagct ccacgtagcg ccgcaggcac tcgtcgcgtc
    50521 gagccaggat ggtgcgctcg tcgacgccgg ccagccgcat gatctcggtc acgcactccg
    50581 gatcggtcat gcccgcgaag cgaaagccct cgatgggtcc ggcggtctga taggtgtcgc
    50641 gcagcgcccg ggcaaaggcg atcttggccg cgatgccgtc ccgtaggagc gtgccgtcga
    50701 tgtcgaagag gatgagtcgc atgagggggc gcggggcgcc ccccagaagt atcacgcggc
    50761 ccgagggccg gtcaacgcgc ggcggcgggc tgcgccgctt gagccgcggc catcccgctc
    50821 tcgaagagct tcgggttggt ggaccgtgag agcgcggcct gggccgtgat cttgcccgag
    50881 gtcacgagat ccttgagcgc ctggtccagc atgaccatgc cgtccttggc tcccacctgc
    50941 atcatgctcg ggatctgggc ggtcttgccc tcccggatca ggttccggag cgcgggcacg
    51001 ccgaccagga tctcgtgcgc ggcgacgcgc cccttgccgt cggcggtcgg gatcagggtc
    51061 tgcgcgacga ctccctcgag cgactcgctc agcatcgttc ggatctgtgc ctggcggtcg
    51121 gcggggaaga tgtcgacgag gcggtcgatg gtcttgggcg ccgagttcgt gtggagcgtg
    51181 ccgaacacga gatgtcccgt ctccgcggcg gtgatcgcga gcgacgtcgt ctcgagatcg
    51241 cggagctcgc ccacgaggag aatatccgga tcctcgcgga gcgcgccccg aagcgcggcc
    51301 gcgaagctcg tcgtgtgcgc gcccacctcg cgctggttga tgaggcatcg cttccgctcg
    51361 tgaacgaact cgagcgggtc ctcgatcgtg atgatgtgct tggggaacgt ggcgttcacg
    51421 tagtcgatca tcgccgcgag cgtcgtcgac ttgccgctcc cggtcggtcc cgtcacgagc
    51481 acgagtccgc gctccttcga cgcgagatcg cggaggatgg ctggcccctt cagctcctcg
    51541 agggtcggga ccttcgtcgc gatgacccgg aacacggcgc cgacgccgcg gcgctgctcg
    51601 aaatagttcg cccggaagcg ggagagcccc gggatttcga tggcgaagtc gagctcgtgc
    51661 cgctcttcga agtcggcctt ctgggccggg gtcagcaagg agtagagcgc gtcccgcgtc
    51721 tccttcggat cgagcggcgg cgtctggaac ggaacgatgt cgcctcgcat gcgcgccatg
    51781 ggcggcgacg ccgtcgagat gtgaaggtcg gacgccttcg cctcgacgat cgccttgagg
    51841 agcgcctgga tgtcgacggg catcagcgtc cctgaccttt ccagaggcgc tcgcggtcct
    51901 cgtcgctcag gatcgtgact tcgagaatgc cggcccggtg ctcggcctct tccggctccg
    51961 cccacgccga ggagccttca tggcgcgcgg tcggcagcgc gatcatcgcg aacggacgat
    52021 cgcgcagcac ggcacgcccg ttcgggctgt ctgcgaggca ctggaggagg gctttcccgt
    52081 ccagccatcc cgtgcgggcg aagacgagcc gccccaccgc ggctccgtcc aggagctccg
    52141 ccacggaatc gccgtgaagg aggtcgtcca gcacgacgat gtccgctccg agaccgaccg
    52201 aggcacgacg ccagttcacg ctggaggggt ctccggcctg gaactggatc cgggtcactc
    52261 ccgcgggata gggcgccgtg ggccacggct cgaaggcgac gacgcggcgc tcgggcttca
    52321 tggcgagaga cgcgagcgag gcgagcacgg tcgagccgcc cgcccgcggc gggccgtaca
    52381 cgatcacgag cccctccggg ccctcgacga attcgcgaat ctcgccctcg ccgagcggag
    52441 agagcccgag cagcgagaga tcgggcgcct cggctcgatc ggggtggagt cgaagcaccg
    52501 tcgtcacgcc ggaagcgccg cgagcgtggc agacgccgac gtgcacgcga tcgccgctca
    52561 ggtcggccat cacgtgcccc tggactgcga gctcacccat ctccagatcc ggcgcgccca
    52621 ggtgcgcgag gcgcgtgcgc aggtagagca tggtctccgg ctgctcgacc ccgcggggct
    52681 cgagtccctt gtcggtccgg tagtgcaccg cgatgccgcc cttttccggc acgaagtgga
    52741 tctcggacgc acggtcgttg cgcgcggtat agacgtgata gaggaggaag gtcgatccag
    52801 agcgctccca gaccacccgg tggcgcggct cgcccccggc cgcggccgct atcgtggccg
    52861 cctcggccgc gctcgcgcgc gcggcgacct tgtcccgcga tggccgctcc ggcccgtaga
    52921 cgttgtcgag cacccggcgg atcgacccct ggcagccgat gacgagcgag acgggcatcc
    52981 ccgccgcctg ctcgatctcc ctcacggcct cggagtcggt gggatcggac atcgcgatga
    53041 ccatctcctc cgggttctgg aagaggggca tcgcctggat gcgacgaagg agcgattcgg
    53101 ggaagcgccg gacgagatcc atgtcggcgg cgccggacgc gaggtccaca tacggaatgc
    53161 cgagctgctt ggagagggcc cagccgatgt tctcctcggt cgtgaagccc atctcgacga
    53221 ggctctcgcc gatcaggcgg ccggtctcga gctgctgctg gaacgcggca tccacctgtt
    53281 ccggagtcac gatcccgccg tcgatcagga tcgacgccag ggtccgcttc gtctgaccgg
    53341 tctcgctcac gaggcacctc tgcgtccggg ggttgggaat cgattcggcc ctgggcgaat
    53401 cgcaaggcat gtgccatgcg taccccacgg catggcgcgc cagggccatg gccgccgcct
    53461 tgctcgattc ggagggaacg cggtgcgaaa tggcgagttg agaagcgatg agcggtgggt
    53521 cgcagcccct ggcggggacc tctacgcggc tcccggggcg gcgcaatgct tgccccttgc
    53581 tcggccagcg gttattttct gcgcggtttc catgcagcgc atcgacacct catcggacgg
    53641 gagcccatgg cgcacgcaca aatgaaacgg aaatccgccg acgccactgg aaagaaacgg
    53701 aagtccggct ccaccgagcc cgaagccgcc gctccgtcga agaagcagcc caagatcgtg
    53761 cggctcaccg ccaccgatct cgcggagaag cagcgcgaga tctcggtcag cgagttcttc
    53821 accaagaacc gccatctcct cggattcgac agccccgcga aggccttgtt gacgacggtg
    53881 aaggaagccg tcgacaacgc gctcgacgcg tgcgaggagg cgggcatcct tcccgacctc
    53941 ctgctcgaga tccaggagat ctccgagacc cgttaccgta tcgcggtcca ggacaacggc
    54001 cccggcatcg tgaaggctca aatccccaag atctttggcc agctcctctc tggatcgaag
    54061 ttccacaggc tgcgccagtc gcgcggccag caggggatcg gcatctccgc ggccgggatg
    54121 tacggccagc tcacgaccgg caagccgatc accgtgatct cgcggatcgg cgccgggaag
    54181 cccgcgcaca agttcgacat ccagatcgac acgcggcaga accggccggt cgtcgtggcg
    54241 gatcacgaag tcgagtggac gccgcagcac ggcacgcgcg tggagctcga gatcgaggcc
    54301 acctacaaga aagggcgcca ctcggtcgac ggctacgtcg agcagacggc gatggccaat
    54361 ccgcacgcgc agatcacgta cgtggcgccg acgggcgaac ggacggagct gccgcgcgtc
    54421 gcgaacgagc tgccgaagcc cccgcaggag atcaagccgc acccgcacgg cgtggagctg
    54481 ggcatcctct tgaagatgct ccacgacacg aaggccagga acctccgcgg cttcctgacc
    54541 agcgagttct cgcgtgtgag cgccgcggtg gccgaggaaa tcgccaccac ggccggcctc
    54601 tccggaggca tgaccacgca gaaggcggcg cgcgagcagg ccgaggcgct ctacaaggcg
    54661 atccaggcca cgaagctcat ggccccaccg tcgaacgtgc tctcgccgat cggcgaggag
    54721 ctgttgctcg agggcttgag gaagcagatc gacgcccgct tctacgccgc gacctcgcgg
    54781 tcaccgtcgg tgtatcgcgg gaacccgttt ctcgtggagg ctgccatcgc cttcggggcg
    54841 gagcagcttc cggccgacga gccgatcacg ctgatccgat tcgcgaaccg ggtgccactc
    54901 ctctaccagc agtccgcctg cgcggtcacg aagtcggtga tccagaccaa ctggaagtcg
    54961 tacggcatcc agcagtcgcg cggcgcgctc ccgtccggcc cgatgatcat catggtgcac
    55021 atcgcgtccg tgtgggtgcc gttcacgtcg gagtcgaagg aagcggtcgc ctcgtatccc
    55081 gagatcatga aggagatcaa gctcgcgctt caggacgtcg ggcggaagct cggcatcttc
    55141 atccggcgcg ggatgcggga ggcggacgcg gagaagaagc ggtcctatat cgagaagtac
    55201 atcccgcaca tcgggatcgc gcttcaggaa atcctgagca tcagcgacaa ggaaaaggac
    55261 aaggtctgcg cgaccttgaa ggacacccta gagcggagcc ggaagctatg agcgatacca
    55321 acgtcgttcc caagatcaag aaagaggcga aggccgtcga ggagtcgatc ctcaagaaga
    55381 agacgccctc gttgaagttt cccatccgtt cgctcgcgaa cgtgaggtat ctcgagaagg
    55441 aaggctactt cgagatccgc gggaagaaga aggagcggac gctcacggtc aacaccgtga
    55501 agacgttcgc gcagacgttg agaatgatgg ccctgtccaa gaccttggtc gagacggacg
    55561 acatggcgac caagcgagag gcgtactacg tctccaagaa ctgggacgac gcacggttct
    55621 tggagcagac cgagtccgac acggtgatgg acgacgtcga ggccttgttc gaggtcaacc
    55681 gcgagcagct cggattcgtc cccgaggaga aggggggcga cgtcgccggc accttggtcg
    55741 tcgtggacaa ggaccccgat accggcaagt cgatccggat cgactgcacc aagttcggct
    55801 ccggcgcgta ctcgatcccg atttccgtgg aagacttgaa gttcgagacc aaggccaagt
    55861 tcatcctcgt catcgagacc gccggcatgt tccagcgcct cgtgaagcac ggctactgga
    55921 agaaggccaa ctgcatcctg gtctcgatgg gaggcgtgcc cacgcgcgcc tgccgccggt
    55981 tcatccgccg gctcgccgac tcgatgaaga tccctgtcta cgtcttcgtc gacggcgatc
    56041 cctacggcat cagcaacatc taccgcacgt tcaaggtggg ctccggaaac gcggcgcaca
    56101 tcaacgagtt cttctgcgtt ccgcaggcga ggttcctcgg catcacgccc caggacatca
    56161 tcgactacaa gctcccgacg caccccttga aggacgtcga cgtgaagcgc gccaaggacg
    56221 cggtcaagaa cgacccgttc ttccagcacc acaagccctg ggtgaaggcc atggagcagc
    56281 tcatgagcat gggggtccgc gcggagcagc aggcgctcgc ggcccatggc ttgaactacg
    56341 tgatggacac gtacctgccc gagaagatcg cgcaccctga gcggttcctg ccgtaaggtc
    56401 gggagcggca gcaggcgatt tgtgaattca ttctcaactt gccccggggc aagttgacga
    56461 ggacccatgc cgatccgcgg cgtccatacg atgttctaca cgtcggagcc cgaggcgatg
    56521 cgcgtcttcc tgcgcgacaa gctcgggctt cacggcgtcg acgtcggcgg cggctggctg
    56581 atcttcgacg tccctgaggc ggacatgggc tgccatcccg cggacccgaa cagcgtccat
    56641 ggcgagccct ccgggacgaa ccacatctcg ttctactgcg acgacgtgcg gaagacggtg
    56701 gaggagctga aggggcgagg cgtcgagttc gtggacggga tcgtggatcg cggctacggc
    56761 ctcgtcacgc acttcaaggt gccgggtggg ttcgaggtcg agctctacca gccgaagtac
    56821 gagaagggtt gatcgctcga agaactacgg gtgggccagg aagaagtagt tcagccgcct
    56881 ttcgaactgc tcgatgaact gatcgcgggt cagcggcgaa cggccagcga gccagtccgg
    56941 taagagcgag atgcggagcg gagcctcacc caggacatcg aatatctcgc ccgctccgct
    57001 accgaaatga tccgccccgc ctaggccgca ttcgaagagc acgtgcggtc gttggcgtcg
    57061 cagcatctga agccctcccc ggaacactcc gagctcggct ccttccacgt ccaccttcac
    57121 cagtttcaca ggcacgtccc ggggcaaaac gcggtcgagc gtctcgaccg tgacatcgat
    57181 ctggcgccgg gtttcgttcc ccaggtcccg ttcccgcgcg ctcagcccgc tcaacgccgg
    57241 atgctccacc atgcaatgga acacggccga gccaggttcg gagccgatcg cgtacggata
    57301 cacattcatt ccggggaatc gcgcctgaag cttcgcgaac tgatccggat tcggctcgaa
    57361 cgcgaagtgc ttcacccctg gagcgagtcg catcatgtgc cgcaggtagg cgccctcgaa
    57421 cgagccgaca tcgacgccga tccctccgcg ccgcaacacc cgccgcatga cagcgattgt
    57481 ctgggagtcg tagaggctgc ccttgtgccc ggtcagcttg gcgtggacgt aacggaaggg
    57541 ctcttcccaa cgggtaccga cgagggttcg cttggcgagg tcggcgagca ttcgggaagt
    57601 gtacccgtgc ctggcggcgg cttcctaggg gtcaccctcg cttggcgccg acgcgccacc
    57661 gggtcacgtc accgcaggct ccgcctccgt ctccgcatgc tccgccgggt cccgggcccc
    57721 catgaagggc agccacagca ccatccccag ctcgccgaaa tagaggggcg acatcacccg
    57781 cgataccgcc gcgatgtgct cggggaaggc gatgatcgtc accgaagtga ccacgtaccc
    57841 cacgcccgag gcgaagagca ggtatccgag gatcttgggg aaatagcccg acttgatcgt
    57901 caggatgcca aacgggaaga gccagagtcc ccagaagatg gtgaggatgc gggcgaggtt
    57961 gttgccccag aggatcgagc ccatgaccag cgcgtcgagc tggggcttgg tgaacgcgga
    58021 gagatagctc tcgccgctca ggaggatcag cggcgtcatc cggttcgcga tcgtcacgag
    58081 ctccgctgcg atcgcgactc ctacgagccc caccatcaat cgcgcgagcg cgcggtcgac
    58141 gtcacggaac agccggtaca gcgtcagcgc cacgacgacg aacaagatct gggatacgag
    58201 cgagatcagg atggtcaggc ggtagagcga ggccccgtcg gcaatgttgc gggccgtcgt
    58261 cgcagcgtcg tcggagagga accgggaggg gacgtacatg tagctgaaga tcatcagggc
    58321 ggacatcacg acgtacagga atccggcgag tcgggcggtc gacttgggtg agtccatggc
    58381 ttcctccagc taccggcgaa ttacgccacc gcacccctca cgctagaaat ccgccgcctt
    58441 cgtggagtcc agcggcggcc gccaggtgct atccaccggc gtccccctct cctcgatcca
    58501 gatgtagacg tcggcttcct cctcgtccat gtgcaggcgt tgccccttcg ggagccgctg
    58561 gatcttggac cacagctcgt cgatgttgat gcggtagtcg tgagcgaggc cgatctcgga
    58621 cttgaggttg aaggcgtgca tatagtcgag cgccttcttg ggcacggtca ccgccacggt
    58681 ggccaggtcc ttcccgggct cccagacctc gactcgtacc acggtgtccc tgccgtacca
    58741 ccagggcttg gggttcggga tgtcgaggcg ggccgtccag gcgtagccga tcccgagaca
    58801 gacgaggagg cccaaggcca gggctatcca gaacttgcga ttcatggatc ttcctcctca
    58861 tccgctcggg accggccggg accgcgaagg ggggaccacc gcggagccca cccctcctct
    58921 acgccaccga acgctcctcg ggtttcaggg cgacgctccg ggacccggcc acgccccgcc
    58981 aggccatggc cagggcgagc gccaggccga aggtcgtcac cacggtcgct cccgtgggca
    59041 ggtcgaagtg gtaggaggcg agcatcccgc cgacgctcac gagcaccgca acgatccagc
    59101 cgacgatgag gcggcttcgg gcgcggtcgg ccagcagcat ggcgatcacc gacgggacga
    59161 tcaagtacga gaaaacgagg agcaccccgg ctaccgccac cgactgggtc acgaccagac
    59221 cgaacgtgag gtagaaaagg aaatcccacc agcgcacgcc ggcgcggctc agcccggcct
    59281 ccgccggccg gccgtgcgtg atggcgaaga acggccggcg ccagatgaag tgcaggaagc
    59341 cgatcacgag atagagcacg gcgagcgtcg cgacttcctt gggtgacgtt gcgagcaggt
    59401 tgccgacgaa catgaacttg atgtgctcgg cgccttcggg cgtgcggtcc acgatcagga
    59461 tcgcggcggc cgcggagacg gcgtacacga tgccgatgaa ggcttcctgc gggatgcgcc
    59521 gcttctcggc cttcaagagc gcgagcagac cggcgcccac gacgaccgcc gcgatcgaaa
    59581 ggtagtgcga tgcgggatcg tggagatcga gcccgaacag gaacccgatc gtcgccccga
    59641 gcgccgcgac ctgggcgagc gccagatcga cgaagatcac cccacgctcg accacgtgga
    59701 atccgagata cgcgtgaatg cccgcaatga cgacgcaggc gaggagcggc cagagaagga
    59761 actggaaggc gtccatcacg acccctttcc cgtgagcgcg ttctggatca acccgagctg
    59821 ggcgtcgaag aggcccaggt agctcgtgac ctccttcgtc gcgccaaccg agggaggcag
    59881 gatgacgagc gccgcaccgg tgtttcgggc gatctgctgc ggcatcttgg gatcgaagta
    59941 gggctcgacg aggaggagct tcacgttctg ggctttcatc tccgtggtca gatcctggat
    60001 gtgcttgggc gtcggcggca cgcccggctt cggctcgacg aaatccatca cctcgaatcc
    60061 gaacgcgcgg gcgaagtagg gccaggagcg gtgatacgtc accacgcgcg ttccggtcag
    60121 gccgatcgac ttcgcgcgct gcttccactc cgcctccttg gcgcggaccc gcttctcgaa
    60181 gtccgcgtac cgtgatgcga aggtcggtgc gttggcggga gacacgcggg agagtccgtc
    60241 gcggatcgcc gcggccatga tgattccgtt ctcgggatcg agccagtagt gggggtttcc
    60301 gagcgggtgc acgtcgcctt gctcgcgacc gaccggcccg ccgagctcga ggacctggat
    60361 gttcttcgag gcgtccacga aaccggcgcc gccggggagg atcttcgaat tgcgcgcgct
    60421 gttgaggagg ccgggcaccc agcccacttc gagatcgcgt cccacctcga cgaagagatc
    60481 cgccttcttc agcttcacga gatagctcgg cttggcatcg acgaagtgcg gatcctgata
    60541 gcccctcgag agcgactcca cggtcacgag atcgccgccg acctcgttcg tgatcgaggc
    60601 gaggtcctca gtggtggtga cgacgttgat cttcttgtcc gcgcgcgcag cgtggggcgc
    60661 ggtcatgagc gcgagggtga gcagcggtag cgcccaccgt tttgtgaatc gagtcatggt
    60721 cggtctccta tctagaacgg gtgctgcccg tgcgggccga tgttgaacgt ggtcttgaag
    60781 aagtagctca tctcgcttcc gtcgaagtcg cggtcctgcc cctggaggct cagcgaccag
    60841 aactcgctcg gagtgaacgt gaggaagccg aggccgccgg tcgtcgtgtc gtccgcgatc
    60901 gcgtcgatcg cctgcgacca gtcgtagcgg gctccgaggt gccagcgccg ggcgaactga
    60961 tagtcggccc aggcgaaccc gccggtgcgg cgaggtccgt cgtcggggtc gcgagccatc
    61021 cactcgaact gcgccgtgag cgagcgatag atcgcgcgtt tcggattctt ccaacggaac
    61081 gtggcgtccg cgccgtagag ctgggcgcgc cgcgcgtcgg gaaccgcgag cggatccacc
    61141 gtttccgccg gcccgttcgc gccgttggcg tacgagccgc ccaaggtgag attggtcgtc
    61201 tcgccgagat cgaagaacgt cgacgcccgc gcgagataca ggaggtcctt cccgcggtac
    61261 tcggcgaacg ccggcacgct cgtcgagtcg ggcgtgttcg tcacctcgcc gtcgaagttg
    61321 aggtagaagg gaagcggcgc gagccaggac accgacgccc caattccaga gagcccttcc
    61381 tcaccgagga accgctccgc cgcgagcgga cgatccgcga acggcgtctc cggcgggtgc
    61441 gtgcggttga actttccgaa gttgctccgg aatttaccca gcttggccgt gaatcccgcg
    61501 ggcaacgtga ggagcgtgag gtacccctcc tcgacgtcga tcccttcgtc cggactcacc
    61561 gagatgaaga agtcggcgcg gctgtacggg tcgacggtgg cctggtatcc cagctcgacc
    61621 tcgcggagct ggaacgcgct cgtgtcggtc cggtcgagcg cgtcgccggc ctgaccctga
    61681 aaccatccga tcgcgctgat gttcgggttg agcaaattcg agcccgcggg cggcgccgtg
    61741 acgccaccgg acgccggcga atccacgggc gtggtttgcg tcgcggccgc gtcgggcgcg
    61801 gacaccgtgc tgtcggagaa cgaggacgat tcctgagcga tcgcgaagcc cgcggcgctt
    61861 gagaccagcg cgacggcgag cacggcgatc gcccatgcgt ggtaacgcat gaacgtgcct
    61921 cctctattcg attgtcgtgt gaagcgtgga ccggcggacc gcgccgggaa atcggggaga
    61981 gctaggtctg aggaggtccg cgtcccgtgg gggccgcgag gaggagtgcg gggctttcga
    62041 cgacgacggc gtcagcgagt cgctcgggag gcgaatcgag tcgaggcggc tccagcggcg
    62101 agacgacgtc cacctggctc gcctgcaggt ccttgcaaat ctgacacggc gggcccgggt
    62161 gctcgtggag cacggccgat cccagccacg ctccgaagag gagcagggcc aggagggcca
    62221 tcgcgcggga tgtcgttcga gagcgcatca tcgtgtccaa ccgtaccagc gacccgccgg
    62281 ttcctgcaac cgtccgggcc ctagcccttc cgggccgcca cgatcatgcc caccccccag
    62341 acgatgagga gcgccggcca gtagcggagg aggttcccgt ggaggtatcc gaactccttc
    62401 aggaggagga ggattccgac cgcgatcagc gtgaggggaa acgcgatgct catgcttcct
    62461 ttgccgggca tcctcggggc tcctgggtgg tggggtggct tcgccgctaa ttcttcgggg
    62521 gctcttggcg ctggacgcga atccgcatga gatggccgcc gacgtggatc acggtcagcg
    62581 gtcccttgcc ttccgcggtc cagaaggccg ccttgccctt cacgcgtccg ctcacatgct
    62641 ccgtgaagtc gttggtgaac gaatgccagg tgagcgggga gtcgtattcg acgcgcgccg
    62701 cggcatccgg gggcagcgtg atctcgacat tcgagaggag tccgtgcacg ttgatcattc
    62761 gcggccggac ctgacccacg gcgaggcgca cggacgagct gatggccttg atctcggctt
    62821 cgtcgagggc gtcctcacgg agattgatcg tcccgaacgc gaagtaattc gatgagcgaa
    62881 gcgacacgcg ctgatgctcc gggagcctcg cgtccacgat gctgccgaag aggctcatgt
    62941 ccccggcgat gggcgtcgtg ggccacacca ggtagcccac gccgttcttc gcctcgtagc
    63001 cgtgcgcgat catgtccttc ccgagaatgc tcttggcgtt catgcgcgct tcatcgggat
    63061 cgccgggctg tgtatggatc gcgaaggaac ctcccaccgc gcgctgctgg acccggagca
    63121 tgcggatgcc gggattcgtg aagttgatca ccgtggcctg cgtcggcggg aggcccatct
    63181 ccgtgtgctt ggagatccac catcccccga acacgaaggc ggccgcgacc agggcgagtg
    63241 cgaaaacgcc gagaagtgcg ttcttgagag cgttggcgag gagggcgagg ccgaagccga
    63301 cgaggagcac cggccagaag cgggcgagcc acggccagac gccatggggg agatagccca
    63361 gggccgcgcc gagaaggagc gctcccattc cgaccaggaa gaacccgaag acgagcttcc
    63421 cgaatcccat gtggcactac gggtaccacg cgggcgagcg ggttgcaagc agcgagagga
    63481 cggctgagtg cgcggcccaa ggagatcgag gtgagagcgg gatcgagccg ggcaagacac
    63541 atcgtgggcc gtggaatggc gctggctgcc gccgcgatcg tggtcgcgac ggctggctgc
    63601 atgagcggcg tgcgctcgcc catggagcac gagatcacga aacgggtcac ccccgagaag
    63661 ccggaattcg atatcgaagg ctggaccgac agcacgggaa cccaccacaa gctccacggc
    63721 cgcgcccgaa tcgtcggcga ttcgatccag ctctaccagc ggaactacgt gaccaacgca
    63781 aacccaacgg ttaccaccaa gctggtggcg accgtactga ggtcggacgt cgcggaagtg
    63841 accgtcgaaa ggttcgaccc ggtgaaaact gggatgctcc tcgccacacc agtgttgctc
    63901 tacgggctgc tcatcctgat catcgtctca agtgggtacg agccgtaagt agctgtctta
    63961 tagcggctaa cccgccatta cccacgccat atcgtccaaa ttaaggtacc taaacttttt
    64021 cttgcctcgc cggattcgag cgggcattct ccccgagttg ctagtgctta actttttcgg
    64081 ctgctgacga ggggcggccg gcccggctct cggacccgaa aggaactcga ctccccgcat
    64141 gcgctccaag cccttcgccg ccgccctgct cgccgccgcc gtactgacga tgacttcgag
    64201 cttcgccgcc gcccaggact ccatcgagga caagctccac ttctccggct acggcgaggt
    64261 ccattacaac agccacgagc tttcggagaa tggaatcatc gagaacgatc cgcccgccga
    64321 ggtcgacgtg catcgcttcg tgctcggctg gcgctacgac ttcaccccga agatccggat
    64381 ggatgcagaa gtggacttcg agcacgcggc gacggagatc gagctggagt acgcgtacct
    64441 cgaatacgat ctcgcggcgg cgaccagcct ccgcgccggt gcggtgctca tgccgatcgg
    64501 gccgctcaac gagttccacg agccgcccaa ctactattcg gtcgagcggc cgtacgtgga
    64561 gagctcgatc atgccgacca cgtggcagga gatcgggatc ggtctcgtgg gacggctcgg
    64621 cggcggaggg ttagggtatc gcgcgtacgt ggtcacgggg ctcaatgcct ccggtttcga
    64681 tacgttcaac ggcatccgcg agggacgcgg acacggggcc gaggcgcccg cggaagatct
    64741 cgcgggtgtg gcccggctcg agtactcgac gaagaacggt ctcaacctcg gcggctccgc
    64801 ctactacggc ggcgcggacc agggggactc gaccctcggc gacgtcaccg tcacgatcct
    64861 gaacgcggac gctcgattcc gacgcgccgg attcgacctg aagggcgtcc tctatcacga
    64921 gtccgtcggc gacgccgacg tgctgagcaa cgagctggga accacgattg gagagtcgat
    64981 gctcgcctgg tacggcgaag cggcgtacga cgtcctgcgg cgggaccgcg agggatcgag
    65041 cggccgcgcc cttttcctat tcggtcgcta tgaagacttc gatacgaaca cggacgttcc
    65101 gactggcttc gtcgccaatc ccgccgccaa ccgtaccgtg atcaccggcg gcgtctccta
    65161 catgccaatc gacaagatcg cgttcaaggc ggacgtcgag cactggacgg acgacaccga
    65221 cgccgatctc acgcgcttca atctcggcgc tgcgtttcag ttctagatga aggagagcac
    65281 catgctttcg aaacttgcga agtccgcgct tctcgccgcg gtcctcggta acgtctcgat
    65341 tccggccgcc caagcgggca cgccccgcgc cgcgcaggct ggcgggccgg cggcggcggc
    65401 gacggagccc gctccgttcg cccgcgtcta tcttgccatt cgcggctgca cgtcctgcgc
    65461 gcactgccgc accgcgatcc gccagatggt gaacacgaag tccgccggcg gcgaagcgcg
    65521 cctgagcgct gatcaggtcg aggttcgata tcctaagccg agcccggtgc cgctcaagga
    65581 cgtgatccac tcgctcgcgg agaaccgcct ccacgatctg agcctcgtcg acgtgctctt
    65641 cgaagcccgc gggacgatcg ctccgggtcc gaacggcacc gcgaccttca agctctccga
    65701 gacggggcaa gcgttcccca tcacgatcga ccgcgcggtg cgccggccgg tggacggcac
    65761 ggccgtgcac ctcacggcgg tcgtggacgg atggcgaacc aaggacaagt cgggcctctc
    65821 gctcgccgcg cgtacgatcg agacgaactg acgggacctc gttcccgggt tgacaagacc
    65881 ggggcttgct ctcagaattc gcacccgtcc aaggacttac aaggaggtgc cgtggatgat
    65941 ctgaggggca agtcggcgct cgtcaccggc ggaacgcgtg gaatcggcaa ggccatcgcg
    66001 ctcgcgctcg cggacatggg cgcggacgtc gcgatcaact tcttccgaag ccgcgaatcg
    66061 gccaaggccg ccgtcgcgga gatccaggcg aagggcgtca aggctctcgc ggtgcgcgcg
    66121 aacatgggaa acgaggaaca gatcccaccc atgttcgaag agatcaagaa gagcttcggc
    66181 aagctcgaca tcctcgtctc gaacgccgcg ctcggacact tcggcaacgt cctcgaggtg
    66241 accgacaaga tgtgggacgt ggccatggcg accaacgcca aggcgctcct cttctgcgcg
    66301 caagaagccg ccaagatgat gccggaaggc ggcaagatgg tcgcactcac ctcgctcggc
    66361 tcgcaccgct acatcccagg gtacgccgcg atcggcgtct ccaaggcggc catcgagacg
    66421 ctcgtgcggt atctcgccta cgagctcgcg ccgcggaaga tcaacgtgaa cgcggtgtcg
    66481 ggcgggttca tcgacaccga ctcgctcaag atcttcccga gctacgacca gatcatccgg
    66541 gaatcgaccg cgcggacgcc gttcggacgg gtgggcacgc cggaggaggt cgcgaacgtc
    66601 gccgcgttcc tgtgcaccag ccgggcatcg tgggtaacgg ggcaggtcct gatcgtggac
    66661 gggggctaca cgctggggta ggacgctagc ggtggcgcgc cgaccaccaa cccgtaaggc
    66721 gcgtgcttcc caagaacgcg gccagcttct ggtcgaagag cagaaacgcc gtgatcccga
    66781 gattcacgat gctcgcgacg acgacgtatc ggacgagcgt caccattccg ccgaattcgg
    66841 gcggccgcgt gatgaggtag gccagaaccg cgatctggct cagcattgcg aacaccaccc
    66901 cccacgatcg cgcggggcgc tttcccagcc acaaagcgcc catcacgtag aagttccccg
    66961 ccgtgagcac tgcagagaag agaaggctct ggatcgcgac ctggagcgcg tagccgaaga
    67021 ggagcccttt ggtggcctgc gcgtagacgt agaaaccggt tccgaggatc ccgcgcagaa
    67081 gctcctcggc tcccttgatc gcccagagcg cggtgagcgc ccagacctgc cacgggcgat
    67141 ccacggtgcg agcctcccgc atgcggcggg cctcgatccg ctcgcgagcg cccccgggcg
    67201 aagccatctc ggctacaccg ccgccatcac ggtgatcgtc ggctcgtgcc gtacggggaa
    67261 gttcaccgag gcggcgatga agcattcggc gtgcgcgtcc tcgtgcagcg cccgcgcctt
    67321 ctcggcatcc gagccggccg cgagtgtgac gcgcggcttc aggagcacgt ccgtgaactt
    67381 gaacgtccgt ccgcccgcgc gctccatcac gccggaggcg tcgtcctcgt aggagatcac
    67441 ctggatcttc gcgcgcgcgc agagcgccgc gtagctcagg aagtggcacg cggagagcgc
    67501 cgcgacgagc agatcctccg gatcgtgaat cgccggatcc ccccggaaca cggcggccga
    67561 ggacatcttg agcgagggct tgcccgggaa atcgacgcga acctcgcgcg agtacgtttc
    67621 gtacttcacg atcgggccct cgcgggcacc ggtccagacg agtctgcaat cgaagcgatg
    67681 ggacgggggc atcggtggtt cctcctgcgg ccctaggcca cggcggtcct ggcgtgcgcg
    67741 gtctcgagat cgcggtcgag cgagcgcggc agccgccggc gcaaatccag gaatccgacc
    67801 gcggccagga acgcgagcgc catgatcacg ctccagacga tgagcggccg gccgtggaac
    67861 gagtcgagaa gggcgccccc cacgaccggt ccgagcgacc acccgatcga gttgaagacg
    67921 ccgaagagtc ccatgtagcg cccccgcgtc gtctcgcccg agaagttcgc cacgagctgg
    67981 agcgaggggg gcgtcgcgat gatctccccg agcgacacga cgaacgtcgc gacgatcaag
    68041 agcgccatcc cccggcccca acccatcatg ccatatccga ggccgtaaag gaccgcaccc
    68101 agcgcgagcg acgtcgtgag ccggagcgtg cgcgtgagct tgaccgcagg gaactggagg
    68161 aacaccacag tgagcccgtt cagggcgtag agcgtgccga gctgtatctt ggtgagcccg
    68221 ccccactcca ccgcgtagac cgacagcgtc acgatgagct gcgccatcac gacgaagagg
    68281 accgtcgaaa cgaggagata gcgaaggaaa ttcggctcgc ggagcgccct cagggattcc
    68341 tggaattgga atttgggcgg cgcggcggcc tgcccgatgt cggggatctc cgcgatgccc
    68401 ggttcaggtc gtggcctccg gcggcgatgc atcggtgacg tctcttccac tgcgaacgcc
    68461 atgatcgagg ccgcgccgag catgacgccg gagccgacca ggaagaggga cgagtaggag
    68521 agcgccgcga gaaacccgcc gatcgcgggt ccgagcgccc agccaaggtt gcctgcgatc
    68581 ctgagcacgc cgtagtactc cgcctggcgg tccgccccgg ccatgtcggc gaccatcgcg
    68641 cccgacggcg gctcgaacat gcgcccgccg aacgcgctca acgacgtcgc gagcacgaag
    68701 acgtagaacg gtgcgtgcgt gagcacggcg aaaccgagcg atgcgaacgc ggccgcgcgg
    68761 aggagctgcg acgcgatgag cacgattttc cggccgcttc ggtcgctcca ctcgcctccc
    68821 acaacctggc cgagcgcgcc cccgagcgcc gcgatgagga agagcgcgcc caccgcggtc
    68881 atgggaatgc cgcgcgtgcc gtggaggtag aggctcagga acgggaggac gatcgcgaat
    68941 cccgtcgagc tcaagacgcg cccgacgaag aggtgcagga gccgccgatc ctcctcgaag
    69001 aggagccggc gggggaaaag ggtgtcgatc cagcggagca tggccaagcg tatcacaaac
    69061 gcagtaggct cccgccgatg ctgccggtcg atggtgtcgt cgcgggatac aacctcgtcc
    69121 tcgcactcgt gtggctcgga cttcgcgacc gcgcgccaca cgcgtgggtg gtcgcgctgt
    69181 gccatctcgt cgcggcgggc ttgccgtggc tcttcgctgg ggcgcgcccg cggggcgtgt
    69241 ggcgcgcggt ccgggaaggc tatccgctcg tcggatttgc cgtgttctgg ggagagctgg
    69301 gcgtcgtgca gcgcctgcgc ggactgccgc cgcacgacga ggcgatccgc gcgctggatc
    69361 tccgtttgtt ccacgcgcac tggagcgatc tgtggatggc acgcatgcct taccttgcgc
    69421 tccaagaggt gatgttcttc tgctacttcc tctactactt cctactggtc gtgccgcccg
    69481 tcgcgattgg cgtggcgcgc gggtggaagg cgttccgatc ggtcgtgctc gcggtgatgc
    69541 tcacgtatct gagctgcttc ctcttctaca tcgcgtttcc cgtctacggt ccgcgtgcga
    69601 tggccgcggg catggccacg ccggcggtcg gcttcttcca cacgctcgtc gagcgtgcca
    69661 gagagagcgg ggactcgctc ggcaccgcct ttccgagctc gcacgtcgca ggggttgtga
    69721 ccatggcggt gatggggtgg cggtggctcg gccgtggctg ggggttcgtg ctcgcggtag
    69781 ccgccctggc cgtcgctctc tcgacggtct acacgggaaa ccactacgtg gtcgacgcag
    69841 tcgtgggtgc cctctgggtc gtgccgctcc agctctggat ccagccgatc ttggagcgaa
    69901 gggacgtagg actccctcgc ccggaacggg ccgacgcaga gctgccccgc tcgctataat
    69961 cttcggtccg ccatgagccc cgagccgaac gacatccgga tggacgacct ccgtccctgg
    70021 cgcccgcccg cccgcgggga ggcgctcgcg cattacccgg ccacggacaa ggtcctgccg
    70081 ctcggggaag cgctcgctcg gttcgtccct gacggctcga ccgtcgccct cggcacgtgc
    70141 ctcgagcaaa tgatcccctt cgccgccgcg cacgagctga tccgtcgcgg cgcccgcgac
    70201 ctcacgctcg tcggccccgt ctccgacatc gccttcgacc agctcatcgg cgccgggtgc
    70261 gcgcgccgcg tggcggccgc gtgggtcggg aacgtgatga tggggatcgc ctactcgttc
    70321 cggcgcgctg tggaacgggg agcgctcgag gtggtcgagt tctccaactt cacgctggcg
    70381 caggcgctcc acgcggccgc gatcggcgct ccgttcatcc ctacccgctc gacgctgggc
    70441 accgatctca tcgcgcggaa tccctggctc caaccgatcg aggatccgct cggcggcggg
    70501 ccattgctcg ccgtgaagcc gctccatccg gacgtgacga tcgtccatgt gcagcgctcc
    70561 gatccgcacg ggaacgcgca gatgtgggga agcctgggta tcacgctgga cgcggcccgc
    70621 gccgcgcgcg cggtgatcgt gacggcggag gagatcgtcg cgcccgagca gatccgccgc
    70681 gatcccaatc gcgtggtgat tcccgggctc gtcgtcgcgg ccgtgtgcga gtcgcggtgg
    70741 ggcgcgcatc cgtcgccggt gcagggctac taccggcgcg atcacgaggc ctactccgac
    70801 taccacgccc gcacgaagac cgaggaaggc tacgccgcgt ggcgagggga gtgggtggac
    70861 gccgtgcgcg atctcgccgg ctacctcgcg cggctcggaa acgagcgcgt ggcctcgctg
    70921 attccgctgg agcaccacta cgcggagccc gtcgactatg ggtactgacg cgaggtcact
    70981 tgccccgggg caagtcgtcg agcccgttgt gcgcccgcta cctcccgagt ggacaccgct
    71041 ccgggcactc gagatcgtgg gccgtgaccc ctacacgctc ttcttcgaga gcggcgggcc
    71101 agccggtgag gcgagcgagt ggacgatcct cgccttcgat cccctgtacc ggctcgtgct
    71161 gcgcgatgga cggcttcgac ggatggaccc cggcggctcg gatcacgagc tcgacgggca
    71221 tccgctcgac gcgctagcgc gtgcctggcc ggcgcctgtt cacgcggctg agggccaggc
    71281 cgccgagatc ccgttcatct ccggcctcgc gggctacctc tcctacgacc tcaaggattg
    71341 gatcgagaga tatccatccc acgcggtgcg cgatctcgac ttcccgggcc tctcgctccg
    71401 gttccacgac gtggtgtggg cctggaagcg gagcaccggc gagggttggg tcgtttcgac
    71461 cgggttggcg ggagacgaag agggccggac cgcgaatctc gcatttcgcg ccggcgcccg
    71521 tctggccgag cagtggatct tgttcaaaaa ggaaatgggg cgcacacccg agcggacttc
    71581 ggttccgacg cggccgacca cggtcacgtc cacgttcacc cggctcacgt acgaatccat
    71641 ggtcgcgcga gcactcgagt acatcgcggc tggtgacatc taccaggtga acctggcaca
    71701 gcgattccgc gtcgagcccg tgccgccggc cctggatgtc taccgcgcgc tgcgcacgat
    71761 cgcccccgcg ccattcctgg cttttgcttc gcttcccgac ggcggcattg ccagctcctc
    71821 accggagcgc ttcttccgca tcaaaggccg cacgatcgag acctggccga tcaagggcac
    71881 gcgcccacga ggccggacgc cggaggagga tgcacgcctc ctggcggagc ttcgcgccag
    71941 cgccaaagac cacgccgaga acgtgatgat cgtggacctg gagcgaaacg acctcggccg
    72001 ggtgtgcgag acaggctcgg tccgcgtccc ggcgctctgc gacgtcgtgt cgcactcgaa
    72061 cgtgcatcac ctcgagtctc gggtggaggg cacgttgcgt gaggatgctc accccgtgga
    72121 cgtgctgcgc gcgctcatgc ccggcggctc gatcacgggc gctcccaaga tccgcgcggt
    72181 cgagatcatc gacgagctcg agcccgtgcg gcgagccgta tacacgggag cgatcggata
    72241 ctgggacgtc cgcggcgggt gcgactggaa catcgccatc cgcaccatct cggtcgtgga
    72301 tgacgtcgcc tacttccacg ccgggggcgg cgtggtcgcg gactcgaccc cggccggcga
    72361 atatgaagag acgctcgtca aggcgagcgg catgatgcgc gcgctcggcg tcgacgcgca
    72421 cgccttggac gaggcgctcg cgcggccgtg aaagccgccg tcgtcgacgc gcaagcgccc
    72481 gtcgcgacgt ccccgctaca cctgcgcgac gttcccgatc ccgatcccgg ccccggcgag
    72541 gtgcgcgtcc gcgttctcgc ctgcggcgtc tgccgcaccg atctccacgt gatcgaaggc
    72601 gacctcccgc cgcacaagct cccggtcatc ccggggcacc aggtggtcgg gaccgtggat
    72661 cgtctcggtc ccggtgccac gcgcttccag gtcggggacc gaatcgggat cgcgtggctg
    72721 cgccacacgg acggcacgtg ccggtattgc cgctccgcgc gcgagaatct ctgcgaggca
    72781 cagcgcttca ccggctacga cgcggacggt ggctatgccg agtacgccgt ggtgccggag
    72841 gcctttgcgt accggattcc cgacggtttc ggcgacgccg aggccacgcc gctcctctgt
    72901 gccggcatca tcggctaccg ggcgctgcaa cggagtgaaa cgccgcgcgg cgggaagctc
    72961 ggcatctggg gcttcggctc gagcgcgcac atcacgatcc aggtcgccct cgctcggggc
    73021 tgcgaggtct tcgtggccac tcgaggcgaa aagcatcaac agctcgcgcg cgagctggga
    73081 gccgcgtggg tcggcggcgc cgcggacccg cttcccgtga aggtcgacgg cggaatcctc
    73141 ttcgcacccg cgggagagct ggtccccgtt gcgctccact cgatcgagaa gggaggatcg
    73201 ctcgccgtgg ccggaatctg gctcagcgac attcctgcga tgaattacga gcgggagctc
    73261 ttctacgagc ggaatctgcg gagcgtgacg gcgaatacga ggaaggatgg ggaagcgatg
    73321 ctcgcggagg cggcagccgt gaggctgcgc ccccgcgtca ccgaatttcc gctcgccgag
    73381 gccaaccggg tgttgcagct cgcccgggag gaccgcctga gcggtactgc cgtgctgcgt
    73441 ccctagatcc ccgcgtccat gaccaggacg agcgcgccgt tcttgacgtc gaaatcgtac
    73501 gtgccggacg agccgatcgc gtagagcgtg aagttgccat cgccgtgcct ggcttcctcg
    73561 aacagcgcat ccttccccgc cttggtgaag aggtgcgccg actcgttcca gtcgatctgc
    73621 ttcgtctgat tcccagccag cgagaaattg ccccagatct tcacggcggt cggatccgcc
    73681 ttcatcgacg tctcgtccgt gtagggcagg gtcgtcggct gggtcatcca gacttccacc
    73741 gtgaaggcgt tcggtgtgtt gttcgtgaac ttgcccagga acgcgagatc ggacaggttc
    73801 ttgatgtcct ctttgtgatc ctggtactcc ttttcatccg aaagattgat cggcatcccc
    73861 acgaggccgt tgctcgtgac cgtgccgtcg tccacggcga agtcgagctg gatctggccg
    73921 gacgtgagaa tgcagcccga ggcgagaatt ccaatcccca ggccgaggat cagccaacgc
    73981 agtgatttca tgggtctgcc tcctggctag ggctggaagt tgtactgaag gccggcggtg
    74041 acgtcgccga acttgcgcga cgtttcgtcg gtgaccatga ggtcgagctg gctccggacg
    74101 tgcacggaga tcttctcgtt gaccgagaag cccatcccga gcccgaagtt ccacgtgaac
    74161 ttgctcagct cctcgtccgt ccgctcgagc ttggtgctgc cgatgccgac gtacgggtag
    74221 aacttgaatc cggtcgcgag ggtgttcgcg aaaaggaaat tcgcaccgat cgcctttcga
    74281 tcccaaccct cgcgtgaata cgagaggccg ttgaaggtct cagtgcggtc gtcgtacgac
    74341 gactgagcgt agtacggctc gatggtgacg accgggatga ctctgaccgg aacgcgaacg
    74401 ccccattcgc cgccggtttc gccgagggcg tcaccggggc tgaacgagct ggcgtccacg
    74461 tcttggagga cgggagcgct gaggccgtag aaagccccca cgccgacgcg caactcctcc
    74521 gtcgccatcg ccgagcccgc tcccaggaga gcgactgccg cggcgagcgc gagccaacgc
    74581 ttcatgattc ccccttatgt gctgtgagtg tgccgcggtc gctccggcgg gtctccatcc
    74641 atgctctcgt ctggccgggg atacggcggg gggaatgtag catgggagaa ggtccggcac
    74701 catgactacg ggtccctacg tggaccgaaa ggcttatccg gctgagcgcc ggtgggttat
    74761 cagacctgct ccgccatgcg gtacccgacg ccgggttcgg tcatcaagat cgtcggctgg
    74821 ctggggtccg cctccagctt ctgccggagc tgagccatgt agacccgcaa gtagtgccgc
    74881 tggtcggtgc tggtcggacc ccaaacctcg cgcagcagga attcgtgcgt gaggacgcgc
    74941 cccgcatgcc gggccagcgt ggccagcagg cgatactcga tgggagtgag ctgaacggcg
    75001 gcgccgtgga ccgagacacg ccgccgcgcc atgtcgattc gaaggcttcc ggattcgacg
    75061 atctccgcgc ccgcgtcggg ctccaagcgg tgccgtcgag cggcacgaac ccgcgcgaga
    75121 agctccagaa ccgagaacgg tttggtcacg taatcgtcgg ccccgccgtc cagcgcatcg
    75181 accttctcac cctccttgcc gcgtgcggtg agcacgatga tggggacggt ggaccactcc
    75241 cggatccttc gcatcacgtc gagcccatcc atgtcgggaa gaccgagatc gagaaggatg
    75301 acttcgggag accgagtcgc cgcctgagcg attccgtcgc ttcccgtcgt cgcttccacg
    75361 caggtgaagc cctgcccctc gagggccgct cgcaggaatc tgcggatccc cagctcgtcc
    75421 tcgatgagga gggccacggg cggaacccca gccggcgctt cgctcatgaa tgtgcctcgc
    75481 gcatgctcgc gtcggcttcc gactcgatcg tcggggggct cccctggacc ggcaagcgaa
    75541 cggtgaagac ggccccaccc gttgcacgat tcgcggcccc gatcgtcccg ccgtgcgcct
    75601 ccacgattcc ccggcagatc gcaagcccga gtccggcgcc tccacgatgg gacgccccgc
    75661 cgccccggta gaacttttcg aacacacggg cctgatcctc ctcggacagg ccccttccgg
    75721 aatcgcacac ctcgagccgc agctccccgt cttccaaacg tccatggatg cgaatgggcc
    75781 catcgttgcc cgagtgccgg atcgcgttgt cgacgagatt gcgcacgacc tgctcgaaca
    75841 ggatgtcgtc catcggaatc agggggagtc ccgcgaggga tacctgaatt gtccgcccgt
    75901 tgatttccgg tccgagccgt gtcagggcgg cgccaaccac ctcttcgacc gagtgccatt
    75961 ccttgtggac gcgcatcgcg cccgactcga ggcgcgtcac gtcgagaagg ttcccgatca
    76021 ggcgatggag ccgctgcgcc tcgtccgcga tcgtttccgc gagctcccgg cgcgtgatgg
    76081 cccccaggga ggcgtcgtct cgaagagtcg tcgccgcacc cgtgatcgcg gtcaggggcg
    76141 tgcgcagatc atgcgacaca ctgctcagga gcgcgctgcg ggctcgttcg acctcgattt
    76201 cggattgggt gtggcgtgcc ctatccgcca
//
LOCUS       DASTKC010000170        10897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_850072, whole genome shotgun sequence.
ACCESSION   DASTKC010000170 DASTKC010000000
VERSION     DASTKC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850072"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFR25_07035"
     CDS             complement(<1..518)
                     /locus_tag="VFR25_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9940839.1"
                     /translation="MAVELRVPEVGESITEVQIGTWLKNEGDLVERDEALVEIESDKA
                     TLELPAPVSGTLSQVLKKKGESAKVGDLIATIGEGKGDGARKASATAAPKPASAAAAA
                     PAPAPALAKSEPPKPAPAAPQTAKPAPAPARSSAAPPMRGEPRVMPSARRELAQRGIE
                     ASDVQGTGPGGR"
     gene            complement(574..3447)
                     /locus_tag="VFR25_07040"
     CDS             complement(574..3447)
                     /locus_tag="VFR25_07040"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET9940840.1"
                     /translation="MSDFDPQDPNTLSLPFVEGLYAEFLREPESVPEAWGRYFETLAK
                     EANGFASRPQLDPSFPPQRLFGRATQARVESHGDRDGMIGTFVSRAPANGAPATAEAV
                     DTTAEFARSAEALFRSFRARGHLIAKVDPLGAPRPMPSDLEPAYFGFTESDLDRTIAD
                     PISDGAPVPMRQILERLKRTYCGSVGAHYLHIEDTSIREWLQHRMERSQNHAGLSREE
                     QVKVLRRLTEAQVFEDFIQKKYIGSKSFSIEGSESLIPLLDLVIETASGQGQSEIVIG
                     MAHRGRLNVLANVMGKAPRRIFREFEDADPEMYVGKGDVKYHLGYSVDLGTESGRAMH
                     LSLCYNPSHLEYVDPVAVGRMRAKQDRVGDRTGDKGMVLLIHGDAAFAGEGVVQEVLN
                     MSQLAGYKTGGTLHVIVNNQIGFTTLPDEGRSTPYATDVARMLQVPIFHVNGEDLEAV
                     VYVTRLAMEFRRQFHRDVVIDLYGYRRHGHNEGDEPAFTQPELYRTIAARAPVHERYR
                     DELIRLGRVTRDEADGFARAKRAFLEEELTHARNGDARPTVESLTEAWTDYKGGPDAD
                     VPEPETGITLEREAKILEAQASVPEGFHPHPKARRGLELKREMARGEKPLDWAAAESV
                     AFGSLALEGARVRLSGQDSLRGTFSQRHAGLFDTENGALHVALQHLSKDQAPVDIINS
                     PLSEVGVVGFEYGYSLECPAGLTLWEAQFGDFVNAAQVIIDQFLVSGEEKWRRLSALV
                     LLLPHGLEGQGSEHSSARLERFLQLAAKDNIQVVYPSTPAQYFHVLRRQVVRPWRKPL
                     VVMTPKSLLRNPACVSPIADLTRGKFQRVIPDLVVSPADAKRILLCSGKIYYELVAKR
                     ASLKRKDIAIARVEQLYPLAASALEPVVQGAREGTPVVWVQEEAENMGAWSYILARFG
                     RSLFGRYPLSGVTRPEAATPASGALSSHRLEQARLLTAAFDVA"
     gene            3676..5040
                     /locus_tag="VFR25_07045"
     CDS             3676..5040
                     /locus_tag="VFR25_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9940841.1"
                     /translation="MISSINPATGETIATYEAMSPAEATRVVQQADQAFRTWRRLGFD
                     ERAEPMKRAGDLLEERKDALALLMAREMGKPIGQGISEAEKCAWVCRHYAEHAQSFLV
                     PEEIPSDAAKSYAAFQPLGVVLGVMPWNFPFWQVFRFAAPTLMAGNACVLKHASNVTG
                     CALAIGDIMRDAGFPEGLFGVLLVPGEQVARVIEMPLVRAVSLTGSSAAGRSVAREAG
                     RVLKKCVLELGGSDPYVVLEDAELGPAVETCVASRLINGGQSCIAAKRFIVVRSLESE
                     FTERMVDLMREKTQGQPTEPSIDLGPLARRDLRDQLHAQVTESVRRGAVRALGGTMPP
                     GAGAFYPPTVLTSVRPGMPAYEEETFGPVAAVIAAEDEADALRIANDSPFGLGAAVFT
                     KDRARGERIAAEEFEAGACFVNAFVRSDPRLPFGGVKDSGYGRELSSFGIREFVNIKT
                     VYVA"
     gene            5037..6968
                     /locus_tag="VFR25_07050"
     CDS             5037..6968
                     /locus_tag="VFR25_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9940842.1"
                     /translation="MIRLNGIGYSIGTRTLFSDVDWVVGPGDRVALVGPNGAGKTTLL
                     KIILGEVTPEKGTRVVSKGTRFGYLPQEAAEKFEGTVLDRALDAHRHLIEMREELDAL
                     YQEIPTLEPGPEMERLLDRAGELQHHLEQHSEHALEPEARRVLGGLGFSREDQDRPLS
                     EFSGGWRMRAALAALLLTDPTLLFLDEPTNHLDLPALEWLEDYLEDFHGGLVVVSHDR
                     VFLDRVTTEISELDHEELTEYPMSFTGYLEERELRRERIEAQNVQLDKKIAQLERFAE
                     RFGAKNSKAAQAQSKRKQAERLKARRVVLPRKPRGIRFTFPPPPHVGRSLIRLEDASF
                     GYDKRDVFSGASFEIERGEKVAIVGANGAGKTTLLRVIAGQLEPRRGEREIHQAARPA
                     FFAQHAAEILDGTLTVLEAVEEVATDDARPRLRSLLGNFLFVGDDVFKHCRILSGGER
                     QRVAIARLLLQPTNLLLLDEPTHHLDLAGKEVLEEALSQYPGAVVVVTHDRSLMARLA
                     TRILAVRDGRVELYPGGYDDYEAARLAESEGRPPQPSQDGQATPVKAAKQKADHERKD
                     DKRRAAESKKRSQEIEKLEREIEGKESELKALEAQLADPQVYAEPPRSRELLARYEHL
                     KADVEASWARLEALSEAKS"
     gene            7001..8632
                     /locus_tag="VFR25_07055"
     CDS             7001..8632
                     /locus_tag="VFR25_07055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9940843.1"
                     /translation="MAAPKLGTPTGMSTTARSRWRTLPPELLNQACKRVRIMILSVGS
                     LWLIAFFMDSVVARLLMHHHTEGMIMMDRFPANVIGWTGIVSSLGAFFLARRLAGRAH
                     ALINLGVVYQVFTAALVALLTNLTPHLGEGRVSWVCIIILIQPAVVPTPPIKTLVASL
                     IIATVDPLTVWLAHLRGVPIPGDAFTLTWAFLPNYICVGLAMLPSAVIMGLSRQVNEA
                     RELGSYLLGELIGRGGMGEVYHARHRMLARPAAIKLIRPESLGAADMNAAQAMIQRFR
                     REAQAAASLKSPHTIVLYDFGVTDDGVFYYVMELLDGFDLETLVKRFGPVSPERAIHL
                     LSHTCQSLAEAHARGLVHRDVKPSNIFTCRLGLQVDYAKVLDFGLVKVARDAEVGVGT
                     VLMTNPDVTTGTPAFMAPEMALGDTVDHRADIYALGCVGYWLLTGRLVFEADTPVKMM
                     LQHIQSTPKRPSELSEIAIPAELERALLRCLAKKPEDRPQTADELHGLLACVPIAERW
                     TKERAGSWWDVHSPASAPTTKLHDETPIATMHVAK"
     gene            complement(8652..10583)
                     /locus_tag="VFR25_07060"
     CDS             complement(8652..10583)
                     /locus_tag="VFR25_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940844.1"
                     /translation="MSGGALRRWALLAPPLAAIALALPMLRLGYFWDDYYFLTRAQHG
                     LAACLEYVQGAAFYRPLTQGLYFLPLAGAGSAGAIASHLANLVLLAVSAWLLAIIIAR
                     HAGTRAGLLAGLLFAGIAPAASLVGWASGAQDLAAILFFLVALDLHDRGHTAWAVIAM
                     LLALLSKEAIGAALPVFVLWDLLLGRKPARVLVPLLAFGAVAVAWAALHPGIQLLVTG
                     RLNEEPRSYVGFQNHTMVFLEWRRYWRALFNTPATGLGTPWPVDRIAYSAAAWIVGVL
                     ALVLTHRQAGHGGTDALTPRRAVLLGALLTIPALLLPSLVVRRWAAYFVCIPALGTSI
                     ALGVVLARAEAAVSAFALAVYLALGLWSRGVHDPSGALLTERSFIEGERATRDLERKF
                     REFYPTLPHGTQVLVSVAASGMAGTHGTLVDGQAPRIWYDDATIRVLDPERRVAGTPH
                     EILLRVTEARDLIQIDPDIPSVRSSGAPARMPEIRIVIGTYARGVAATGDPDRAVRIL
                     LRLANTEPYLLVRAHDQRLAAMVLYARGDSTGAEQILRSVPPLPRGIAIDAIARVLAQ
                     PSRSPTLDAQAYRVFGISPRDPDALRYWMAMFYGAEYFAQAKEMARRLQEVKPGDKES
                     AEVLAKLQTFPTNPFARDP"
     gene            complement(10580..>10897)
                     /locus_tag="VFR25_07065"
     CDS             complement(10580..>10897)
                     /locus_tag="VFR25_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940845.1"
                     /translation="LKDRANRSLEHVFTLLALALPRQPLKIAFKGLHTTDPLLRGTAL
                     EYLETSLPPDIRRALWPYLEDNRPRRAGPARAPDQVLNDLLDSSASIVIRLDEIRREE
                     KRE"
BASE COUNT         1686 a         3677 c         3856 g         1678 t
ORIGIN      
        1 atccgtccgc cgggcccggt gccctggacg tcgctcgcct caattcctcg ctgcgcgagc
       61 tcgcggcgcg ccgacggcat gacgcgcggc tcgccccgca tcgggggcgc ggccgaggag
      121 cgtgccggcg ctggcgcagg cttggcggtt tgaggcgcgg cgggcgcggg cttcggaggc
      181 tcgctcttcg cgagcgcggg cgcgggcgcg ggcgcggctg cggccgcgga ggccggcttg
      241 ggcgccgcag tcgcggaggc cttcctcgct ccgtctccct ttccctcgcc gatggtcgcg
      301 atgagatcgc cgaccttcgc cgactcgccc ttcttcttca acacctgcga gagcgtgccc
      361 gacacgggcg cgggaagctc cagcgtcgcc ttgtcggact cgatctcgac gagcgcttca
      421 tcgcgctcga ccagatcccc ttcgttcttc agccaggtgc cgatctgcac ctcggtaatc
      481 gattcgccca cttcgggaac gcgcagctca accgccatcc gtgtctcctc ggaaacgtag
      541 ggctctgcat cggccggctc ggccgggcag cgattatgcc acgtcgaagg cggcggtgag
      601 cagccgcgcc tgctccaggc ggtggctgct cagagcgccg ctcgcagggg tcgcggcctc
      661 gggacgcgtg acgccgctca gcggataacg tccgaacaag gacctgccga atcgcgccag
      721 gatgtagctc caggcgccca tgttctcggc ctcttcctgg acccagacca ccggcgttcc
      781 ttcacgcgcg ccctggacca cgggctcgag cgcgctcgcc gcgagcggat agagctgctc
      841 cacgcgcgcg atggcgatgt cctttcgctt cagggaggcc cgcttcgcca cgagctcgta
      901 gtagatcttg cccgagcaga ggaggatccg cttcgcgtcc gccggactca cgacgagatc
      961 gggaatcacg cgctggaact tgccgcgcgt gagatcggcg atcggcgaga cgcacgcggg
     1021 attgcggagg aggctcttcg gcgtcatcac gacgagcggc ttccgccaag ggcgcacgac
     1081 ctggcgccga agcacgtgga agtactgggc cggcgtcgag ggatagacga cctggatgtt
     1141 gtccttggcc gcgagctgga ggaagcgctc caggcgtgcg ctcgaatgct ccgacccctg
     1201 tccctcgagt ccgtgcggca ggaggagcac gagtgccgag agccggcgcc acttctcttc
     1261 gccgctcacg aggaattgat cgatgatcac ctgcgccgcg ttcacgaagt cgccgaactg
     1321 cgcctcccag agtgtgaggc cggccgggca ttcgaggctg tagccgtact cgaacccgac
     1381 caccccgact tcactgagcg ggctgttgat gatgtcgacg ggcgcctggt ccttcgacag
     1441 gtgctgaagc gcgacgtgga gcgctccgtt ctccgtgtcg aagagccccg cgtgccgctg
     1501 gctgaaggtg ccgcggagac tgtcctgtcc ggacaggcgg acccgcgcgc cctcgagcgc
     1561 gaggctcccg aacgcgaccg actccgccgc cgcccagtcg agcggcttct cgccgcgcgc
     1621 catctcgcgc ttcagctcga gcccgcgccg ggccttggga tggggatgga acccctccgg
     1681 cacggaggcc tgggcttcga ggatcttggc ctcgcgctcg agcgtgatgc cggtctcggg
     1741 ctcggggacg tccgcgtccg ggccgccctt gtagtcagtc cacgcctccg tcagcgactc
     1801 gaccgtcggg cgcgcgtcgc cgttcctggc gtgcgtcagc tcttcctcga ggaacgcgcg
     1861 cttggcccgc gcgaagccat cggcttcgtc gcgcgtcacg cgccccaggc ggatcagctc
     1921 gtcccggtag cgctcgtgca ccggcgcgcg agccgcgatc gtgcggtaga gctcgggctg
     1981 tgtgaaggcg ggctcgtccc cctcgttgtg cccgtggcgg cgatacccgt agagatcgat
     2041 gaccacgtcg cgatggaact gccggcggaa ctccatcgcg aggcgcgtca cgtacacgac
     2101 cgcctcgaga tcctccccgt tcacgtggaa gatcggcacc tggagcatgc gcgcgacgtc
     2161 ggtcgcgtac ggcgtcgacc ggccttcgtc gggaagcgtg gtgaacccga tctggttgtt
     2221 cacgatgacg tggagcgtgc cgccggtctt gtagccggcg agttggctca tgttgagcac
     2281 ctcctgcacc acgccctcgc cggcgaacgc ggcgtcgccg tggatcagga gcaccatgcc
     2341 cttgtcgccc gtgcgatcgc cgaccctgtc ctgcttggcg cgcatccggc cgaccgcgac
     2401 gggatcgacg tactcgaggt ggctcgggtt gtagcagagc gagaggtgca tcgcccgccc
     2461 gctctccgtc ccgagatcga cgctgtaccc gaggtggtac ttcacgtcgc cctttcccac
     2521 gtacatctcg ggatcggcgt cctcgaactc gcggaagatc cggcgaggcg ccttccccat
     2581 gacgttcgcg agcacgttga gccgcccgcg atgcgccatg ccgatcacga tctcgctctg
     2641 cccctggccc gatgccgtct cgatcacgag atcgaggagc gggatcaggg actcggagcc
     2701 ttcgatcgag aagctcttcg agccgatgta cttcttctgg atgaagtcct cgaagacctg
     2761 cgcctcggtg aggcggcgga ggaccttcac ctgctcctcg cgggagaggc cggcgtggtt
     2821 ctgcgagcgc tccatccggt gctggagcca ctcgcggatg gacgtgtcct cgatgtggag
     2881 gtaatgcgcg cccaccgacc cgcagtaggt ccgcttcagg cgctccaaga tctggcgcat
     2941 cgggacggga gcgccgtccg agatgggatc ggcgatcgtc cggtcgaggt ccgactcggt
     3001 gaagccgaag taggcgggct cgaggtcgct cggcatgggt cgcggagcgc ccagcggatc
     3061 gaccttggcg atcaggtgcc cgcgcgcgcg gaagctccgg aagagcgcct ccgcgctcct
     3121 cgcgaactcg gcggtggtat ccacggcttc ggccgtcgcc ggggcgccgt tcgccggggc
     3181 gcgcgagacg aaggtcccga tcatcccgtc ccgatctccg tgcgactcca cgcgcgcttg
     3241 ggtcgcgcgc ccgaagagcc gctggggcgg gaacgagggg tcgagctggg gccgggaggc
     3301 gaagccgttc gcctccttag ccagcgtctc gaagtaccgt ccccacgcct ccggaaccga
     3361 ctccggctcg cggaggaatt cggcatagag cccctccacg aacgggaggc tcagcgtgtt
     3421 ggggtcttgt gggtcgaaat cggacataat cctcgtttcg gtcgcgtctt ggatgtttgg
     3481 atggcgtccg cgccgcggag cttcgacggg cgccatcgcg gcggaagagg cgcgcgaaaa
     3541 ggatcgcctt ccccgccggg ccgaatatga tgccagagcg ggacggtggg ttcatcaact
     3601 tagtcgatgg caatagcttc cggccgattc cgaagggccg atgcgggtgg ctccacgaca
     3661 ggggccgagg ggcgaatgat ctcgagtatc aatccagcaa cgggtgagac catagcgact
     3721 tacgaggcga tgtctccggc cgaggcgacc cgggtggtcc aacaggcgga ccaggccttc
     3781 cggacgtggc ggcggctcgg ctttgacgaa cgggccgagc cgatgaagcg ggccggcgac
     3841 ctcctcgagg agcggaagga cgcgctcgcc ctcctcatgg cgcgcgaaat gggaaagccc
     3901 atcggacagg ggatctccga ggcggagaag tgcgcctggg tctgccgcca ttacgcggag
     3961 cacgcgcaat cgttcctcgt ccccgaggag atcccgagcg acgcggcgaa gagctacgcg
     4021 gcctttcagc cgctcggcgt ggtgctcggc gtgatgccgt ggaattttcc cttctggcag
     4081 gtcttccgct tcgccgcgcc gacgctcatg gcggggaacg cgtgcgtgct caagcacgca
     4141 tccaatgtga ccgggtgcgc gctcgcgatc ggggacatca tgcgcgacgc gggattcccg
     4201 gaggggctgt tcggcgtgct cctcgttccg ggagagcagg tcgcgcgcgt gatcgagatg
     4261 ccgctcgtgc gcgccgtctc gctcacggga agctcggcgg ccgggcgctc ggtcgcgcgc
     4321 gaggcggggc gcgtgctcaa gaagtgcgtg ctcgagctgg gcgggagcga tccctacgtc
     4381 gtgctcgagg acgcggagct gggtcccgcc gtcgagacct gcgtggcctc gcggctcatc
     4441 aacggcggcc agagctgcat cgcggcgaag cgattcatcg tggtgcgttc gctcgagagc
     4501 gagttcaccg aacgcatggt cgatctcatg cgcgagaaga cgcaggggca gcccacggag
     4561 ccaagcatcg acctgggtcc cctcgcgcgc cgcgatctca gggaccagct ccacgcacag
     4621 gtcacggaga gcgtgcgacg cggcgcggtg cgggcgctcg ggggcacgat gccgcccggc
     4681 gccggcgcgt tctacccgcc gacggtcctc acctcggtgc gccccggcat gcccgcgtac
     4741 gaggaggaaa cgttcggccc cgtggccgcg gtgatcgcgg cggaggacga agcggacgcc
     4801 ctccggatcg cgaacgacag cccgtttggg ctcggcgcgg ccgtcttcac gaaggaccgc
     4861 gcgcgcggcg agcgcatcgc ggccgaggag ttcgaggcgg gcgcgtgctt cgtgaatgcc
     4921 ttcgtgcggt ccgatccccg gctcccgttc ggcggggtca aggactccgg ctacggacgc
     4981 gagctctcct cgttcggcat ccgggaattc gtgaacatca agacggtgta cgtcgcttga
     5041 tccgactgaa cggcatcggc tactccatcg gcacgcggac cctgttctcc gacgtggact
     5101 gggtcgtggg accgggcgat cgcgtggcgc tcgtgggtcc gaacggagcg ggcaagacga
     5161 cgctcctcaa gatcatcctc ggcgaggtca cgcccgagaa gggcacccgc gtcgtctcga
     5221 aggggacgcg cttcggctat ttgccgcagg aggccgccga gaaattcgag gggacggtgc
     5281 tggatcgcgc cctcgacgcg caccggcatc tgatcgagat gcgcgaggag ctggacgcgc
     5341 tctaccagga gatcccgacc ctcgagcccg gtccggagat ggagcggctc ctcgatcgtg
     5401 cgggcgagct gcagcatcac ctggagcagc acagcgagca cgcgctcgag cccgaggcgc
     5461 gccgggtcct cggcgggctc ggcttctcgc gtgaggatca ggaccggccg ctgtcggaat
     5521 tctcgggtgg gtggcgcatg cgcgccgcgc tcgccgcgct cctcctcacc gatccgacgc
     5581 tcctcttcct cgacgagccg accaatcacc tcgatcttcc cgcgctcgag tggctcgagg
     5641 attatctcga ggacttccac ggcgggctcg tggtcgtgtc gcacgaccgg gtcttcctcg
     5701 accgcgtgac gaccgagatc tcggagctgg accacgagga gctcacggag tatccgatgt
     5761 cgttcacggg ttatctcgag gagcgcgagc tgagaaggga gcgcatcgag gcgcagaacg
     5821 tccagctcga caagaagatc gcgcagctcg agcgcttcgc cgagcggttc ggcgccaaga
     5881 actcgaaggc ggcgcaggcc cagagcaagc ggaagcaggc ggagcgcctc aaggcgcgac
     5941 gcgtcgtcct gccgcggaag ccgaggggca tccgcttcac gttcccgccg ccgcctcacg
     6001 tcggccgctc gctcatccgg ctcgaggacg cctcgtttgg ctacgacaag cgggacgtct
     6061 tctcgggagc ttcgttcgag atcgagcggg gcgagaaggt ggcgatcgtg ggagcgaacg
     6121 gcgccggcaa gaccacgctc cttcgcgtga tcgcggggca gctcgagccg cgccggggcg
     6181 agcgcgagat ccatcaggcc gcccggccgg cgttcttcgc gcagcacgcg gccgagatcc
     6241 tggatgggac gctcacggtg ctcgaggcgg tggaggaggt agcgaccgac gacgcacgtc
     6301 cgaggctgcg atcgctcctc gggaacttcc tgttcgtggg cgacgacgtg ttcaagcatt
     6361 gccgtatcct gtcgggcggc gagcggcagc gggtcgcgat cgcgcggctc ctcctccagc
     6421 cgaccaacct gctccttctc gacgagccga cgcatcacct cgacctcgcg gggaaggaag
     6481 tgctcgagga ggcgctgtcg caatatccgg gcgccgtggt ggtcgtgacg cacgatcgct
     6541 cgctcatggc gcggctcgcg acacggatcc tcgcggtcag ggacggacgg gtggagctct
     6601 atccgggcgg ctacgacgac tacgaggcgg ctcggctcgc ggagtcggaa ggacgtcctc
     6661 cacagccctc ccaggatggg caggccacgc cggtgaaggc ggccaagcag aaggccgacc
     6721 acgagcgcaa agacgacaag cgccgcgcgg ccgagtcgaa gaagcgctcg caggagatcg
     6781 agaagctgga gcgggagatc gaggggaagg aatccgagct caaggcgctc gaggctcagc
     6841 tggccgaccc ccaggtgtac gctgagcctc cgcgatcacg cgagctgctc gcgcgatacg
     6901 agcatctcaa agcggatgtg gaagcgtcct gggccaggct cgaggcgctg agcgaagcga
     6961 agtcctgaaa ggagcgacgt tggcgcaatc gaagaagccc atggccgcgc ccaagctcgg
     7021 cacgcccacc ggcatgtcga ccacggcccg gagccgctgg cgcaccctgc cgcccgagct
     7081 cttgaaccag gcgtgcaagc gggttcggat catgatcctc tcggtcggct ccctctggct
     7141 catcgcgttc ttcatggatt ccgtggtcgc gcgcctcctc atgcatcacc acacggaagg
     7201 catgatcatg atggaccggt tcccggcgaa cgtgatcggg tggaccggca tcgtgtcctc
     7261 gctcggcgct ttcttcctcg ctcggcgact cgcggggcgg gcgcacgcgc tcatcaacct
     7321 cggggtcgtg taccaggtct tcaccgccgc cctcgtcgcg ctcctcacga acctgacccc
     7381 acacctgggc gaggggcgcg tctcctgggt ctgcatcatc atcctgatcc agccggcggt
     7441 cgtgccgacg ccgccgatca agacgctcgt cgcctcactc atcatcgcga ccgtggatcc
     7501 gctcacggtg tggctcgcgc acctgagggg cgtgccgatc cccggcgacg ccttcacgct
     7561 cacgtgggcg ttcctgccca actacatctg cgtgggcctc gcgatgctcc cgtcggcggt
     7621 catcatgggg ctgagccggc aggtgaacga ggcccgcgag ctggggagct atctcctggg
     7681 cgagctgatc ggccgcggcg gcatgggcga ggtctaccac gcccggcatc gcatgctggc
     7741 gcgccccgcg gccatcaagc tgatccgccc cgaatcgctc ggcgcggccg acatgaacgc
     7801 cgcgcaggcc atgatccagc gcttccgccg ggaggcgcag gccgcggcct cactcaagtc
     7861 gccgcacacg atcgtgctct acgacttcgg cgtgaccgac gacggggtgt tctactacgt
     7921 catggagctc ctcgacggat tcgacctgga gacgctggtg aagcgcttcg ggccggtctc
     7981 gccggagcgc gccatccatc tcctgagcca tacctgtcag tcgctggcgg aggcccacgc
     8041 gcgcgggctc gtccaccgcg atgtgaaacc ctcgaacatc ttcacgtgcc ggctgggact
     8101 ccaggtggac tacgcgaagg tgctcgactt cgggctcgtg aaggtggcgc gcgatgccga
     8161 ggtgggcgtg ggcacggtgc tcatgaccaa tcccgacgtc acgacgggaa cgccggcgtt
     8221 catggctccg gagatggcgc tgggcgacac ggtcgaccac cgggccgaca tctacgcgct
     8281 cggctgcgtg ggctactggc tcctcacggg gcggctcgtg ttcgaggccg acacaccggt
     8341 caagatgatg ctccagcaca tccagtccac gcccaagcgc ccctcggagc tgagcgagat
     8401 cgcgattccg gccgagctgg agcgggcgct cctccgctgt ctcgccaaga agcccgagga
     8461 ccggccgcag acggcggacg aactccacgg tctccttgca tgcgtaccaa tcgcggagcg
     8521 gtggaccaag gagagggccg gctcctggtg ggacgtccac agccccgcgt ccgcgcccac
     8581 cacgaagctc cacgacgaaa cgccgatcgc cacgatgcac gtggcgaagt agccgctccg
     8641 gcagccgccg gtcaggggtc tcgcgcgaag gggttggtcg gaaacgtctg gagcttcgcg
     8701 agcacctcgg cgctctcctt gtcccccggc ttcacctcct ggagccggcg cgccatctcc
     8761 ttcgcctgcg cgaagtactc ggccccgtag aacatcgcca tccagtagcg gagcgcgtcg
     8821 ggatcgcgcg gtgagatgcc gaacacgcgg tacgcctgcg cgtcgagcgt gggggagcgc
     8881 gacggctgcg cgagcacgcg agcgatggcg tcgatcgcga tgccgcgcgg cagaggcggc
     8941 accgacctga gaatctgctc ggctcccgtc gagtccccgc gcgcgtagag caccatggcg
     9001 gcgagcctct ggtcgtgggc gcggacgaga agatagggct cggtgttcgc gagacggagg
     9061 aggatgcgga ccgcgcgatc gggatcgccc gtggccgcga cgccgcgcgc gtaggtcccg
     9121 atcacgatcc ggatctcggg catccgggcc ggtgcgccac tcgaccggac ggacgggatg
     9181 tcggggtcga tctggatcag gtcccgagcc tcggtgacgc ggagcaggat ctcgtgcggc
     9241 gttcccgcca cgcgtcgctc cggatcgagc acccggatcg tcgcgtcgtc gtaccagatc
     9301 cgcggcgcct gtccgtcgac cagggttccg tgagttcccg ccatgccgct ggccgccacc
     9361 gagacgagga cctgggtgcc atggggcagc gtggggtaga actcccggaa ttttcgctcc
     9421 aggtcgcgcg tcgcccgctc gccctcgatg aagctccgct cggtgagaag ggcgccgctc
     9481 ggatcgtgca cgccgcggct ccagagcccg agtgccaggt agacggcgag cgcgaacgcc
     9541 gacaccgcgg cctcggcccg cgcgagcacg acccccagcg cgatcgaggt gcccagtgcg
     9601 ggaatgcaga cgaagtaggc ggcccatcgc cggaccacga gcgacggaag gagtagggcc
     9661 ggaatcgtga ggagcgcccc gagcaggacg gcgcggcgcg gcgtgagcgc gtccgtgccg
     9721 ccgtgtcccg cttgccggtg cgtcaggacg agtgccagga cgcccacgat ccaggcggcg
     9781 gccgagtagg cgatccggtc cacgggccat ggcgtcccga gtcccgtggc cggagtgttg
     9841 aagagggcgc gccagtagcg cctccactcg agaaacacca tcgtgtggtt ctggaagccg
     9901 acgtagctcc tcggctcctc gttcaagcgt ccggtcacga ggagctggat gccgggatgc
     9961 agtgcggccc acgccaccgc gaccgcgccg aacgcgagga gcggaacgag cacgcgcgcc
    10021 ggctttcggc cgaggaggag atcccagagc acgaacacgg gcagagccgc gccgatcgct
    10081 tccttggaga ggagcgccag cagcatcgcg atcaccgccc atgcggtgtg tccgcggtcg
    10141 tggaggtcga gcgccacgag gaagaagagg atcgccgcaa ggtcctgcgc gccgctcgcc
    10201 cagcccacga ggctcgcggc gggtgcgatg ccggcaaaca agagcccggc gaggagcccg
    10261 gcgcgcgtgc ccgcatgccg cgcgatgatg atcgcgagga gccaggccga caccgcgagg
    10321 agcaccagat ttgcgagatg ggatgcgatc gctcctgcgc tacccgcccc ggcgagcggg
    10381 aggaagtaga gcccctgggt gagcggccgg tagaacgccg ccccctggac gtactcgagg
    10441 cacgcggcca gaccgtgctg cgcccgcgtg aggaagtaat agtcgtccca gaagtagccg
    10501 agccggagca tcgggagcgc gagcgcgatc gctgcgagtg gcggcgcgag gagggcccag
    10561 cgccgtaggg cgccgccgct cactcccgtt tttcctcgcg tcggatctca tcgagccgga
    10621 tcacgatcga cgcgctcgag tcgaggaggt cgttcaagac ctggtcgggt gcgcgcgccg
    10681 gacccgcgcg gcggggacgg ttgtcttcga ggtagggcca gagagccctt cgaatgtccg
    10741 gcggaaggct cgtctccagg tattcgagcg ccgtgccgcg gaggagcgga tcggtcgtgt
    10801 ggagcccttt gaaggcgatc ttgagcggct gccgcggcag cgcgagcgcg agcagcgtga
    10861 acacgtgctc gaggctccgg ttcgctcgat ctttgag
//
LOCUS       DASTKC010000171        12422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_377550, whole genome shotgun sequence.
ACCESSION   DASTKC010000171 DASTKC010000000
VERSION     DASTKC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12422
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377550"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFR25_07070"
     CDS             complement(<1..1146)
                     /locus_tag="VFR25_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9940846.1"
                     /translation="MRPLLIARGWFQAAILVSLFGFFVLGFLALRTYQEEPPIPKQVV
                     APDGRVVFTAADVIGGQQVFLKNGLMEYGSIYGHGAYLGPDYTADYLRRSATIVRDSY
                     EGGGSDRAREQTVRSFKTNTYDPASGVLRFTAEQATAFDELTRHYTEVFSQPTTRFGL
                     RPGAITDPEDLRRLTAYFAWTAWTASTLRPGQDHTYTNNWPPEPLVENRPTADVVVWS
                     VLSLIALLGGIGAMMAVFGRWRVLGWHGREQERLSFRAPGEVALSPAQRATIWYFLVM
                     AVLFLLQSLLGGSVQHYRAEISSFFGVDLARILPFNLARTWHLQLAIFWVAASYLAAG
                     IFIAPMIARREPKGQQWLAYGLLAAVAIVVFGSLAGEYAGVFGYTGRL"
     gene            complement(1225..1698)
                     /locus_tag="VFR25_07075"
     CDS             complement(1225..1698)
                     /locus_tag="VFR25_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940847.1"
                     /translation="MRWFTAAVSIVFVGIGIWMIVDGERIGWAMAGFFGLCLLVAIFE
                     PALAKYRATFDRFHLVITEDEIACEHSVRKRESILWKDVNRIWYITTDAGPRVPDEWF
                     LLESESGGCSFPSEATGLDKFWNEVERRFPGFDFKPVIEGGTDNAKHLCWERKAS"
     gene            complement(1744..2661)
                     /locus_tag="VFR25_07080"
     CDS             complement(1744..2661)
                     /locus_tag="VFR25_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET9940848.1"
                     /translation="MRVGIARTIAAATALAFCLVAAPVIAQELEPGLYHSSPTGLNAA
                     IAGYSYSTGNILLDSSLPIEGAHAKIHNVNLGLIRSFGMFGKTAKVDVQVPIGFGNFE
                     GYVAGVLRTREPTGPADPRVRVLLNVIGAPALSRAEFASYRQGTIVGASVQVVAPLGR
                     YDPERLINLGSNRWSFRPEVGLSRAQGRWFLELSSGAWLFTDNHEYYGGSTLEQSPLL
                     FVKGDVMYLFRRGMWAAVNYGMATGGETTVDDGPASEIQTNERLGATFAYPFGRSFSM
                     KIVYTSGLTTRIGADFDSYGASLQYSWGG"
     gene            complement(2658..4637)
                     /locus_tag="VFR25_07085"
     CDS             complement(2658..4637)
                     /locus_tag="VFR25_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HET9940849.1"
                     /translation="MSTTTAAPERPQAGAERPSTPEQIEREWFENVYAGDRMRQLTVR
                     ALVMGMLLGMIMACSNVYVGLKSGWAVGVAITACIMAWSIFAALHAMLPKLFPPYSIL
                     ENNAMQSCASAAGAITGAGLVNAIPALLMLNPGVLPTGFGARCAILIPWLAVVAWLGV
                     FLAVPTKRQLINVEQLKFPSGTAAAETLRSLHGHGADAARQARALFSALGIGAVITWM
                     RDATAPWMRDVSIRGIPDWSLIKSWVTFPSVPWLRYPRIESVWGTSWARVGTYQGQPL
                     LLNQVTMSFEGSLLFIAAGALMSFRTAWSLLLGAVINYVWLAPIFLATGDITSPSFRN
                     ISRWSLWTGVPMLVTSGLLLFFMNWKSVVRAFSTIGAFFRKSGGEADPMDRIEVPGSW
                     FIGGYALLGTVAIVLGNLFFHIHWWMGLIAVLATFFLVVVAARATGETDITPVGPLSK
                     ITQLTFGAIAPGNVPTNLMTANITAGAVGSAGDLLTDLKSGYILGANPRQQFFAQFFG
                     VLAGALVVIPVFFILIPDPALLGTEKWPAPAALVWRGVAELLAKGPGSLPLSARYGLV
                     IGASLGILLTLLETWLPRLKKFIPSPVGLGLAFTINGFNSVSMFLGALLALGFTKWKP
                     KTSGLYTVPVASGIIAGESLMGVAIAMLSALHILG"
     gene            complement(4706..5449)
                     /locus_tag="VFR25_07090"
     CDS             complement(4706..5449)
                     /locus_tag="VFR25_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9940850.1"
                     /translation="MTSTGPEPVVFIHGLYQGFAHLGDIPFPSSRKLILLDLPGYGGQ
                     VEKAAPSNLSGYADHVASELDRLGASPAIVVGHSVGGAVAMLLAANHRDRVVSIVNVE
                     GNFRPADAFWSTKIAAMTSEEAEAMLDGFKSDVDGWLTRQKIQIKPKRQEWAKRMFDA
                     QPARSVQALARAVVEATASPDYLPTMDSVLLSGVPVHLIAGERSRAGWGVPDDFARLA
                     ASFSIQPKVGHMMPLEEPEAFLALVASKV"
     gene            complement(5475..5870)
                     /locus_tag="VFR25_07095"
     CDS             complement(5475..5870)
                     /locus_tag="VFR25_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9940851.1"
                     /translation="MNQAGGPWPKGIHAITLFVEDLAAARKFYSEAFGLPVVFETEDS
                     AVFRFGSTLVNLLEIAQAPGLVEPAAVARKDAGVRFQFTIEVEDVDAMCAELKRRGVE
                     LLNGPMDRPWGIRTASFRDPGGHIWEIAH"
     gene            complement(5893..6993)
                     /locus_tag="VFR25_07100"
     CDS             complement(5893..6993)
                     /locus_tag="VFR25_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET9940852.1"
                     /translation="MTWIAGSAGLVLVVIILWDAYETILLPRRLPGDIRISRLVLRTL
                     WLSWRALSRRFGASKSRELFLSFYAQLSLILLLSVWAAALLTGFALMQWAIGSHLQAP
                     TGIEGFPADLYLSGTTFFTLGLGDVIPMSPPARVLTVIEAGLGFGFLAMVISYVPVLY
                     QQFARREARITMLDEWAGSPPAAALILRRAFESRDPNAALDQLFRDWEQASAEILESH
                     LSYPTLAFFRSQHDNQSWLASVCAVLDACALTATTVRGIDPFQARLTFAIGRHTLVDV
                     SQVFRLKPRDMDGVRCSPERFHEMCAWLGSSGVPLMEEPDAYKRFLELRHLYTPYLEA
                     LSDYLAMPLPGWLPRAKEKYNWKSTEIATHDQ"
     gene            complement(6990..7616)
                     /locus_tag="VFR25_07105"
     CDS             complement(6990..7616)
                     /locus_tag="VFR25_07105"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9940853.1"
                     /translation="MPAHRWLRPSTLPEGSIDSRTVIFALWFLWGIYWAVAAIGRRRV
                     ARRQPVLERLGHLAVMGIGFALLYAADRRWAGLNRRFLPDSAIVQWIAVLLTILGLGL
                     AVFARVYLGRNWSAEVVIREDHHLIRSGPYAHIRHPIYTGLLLAVLGAVLAIGEIRAI
                     VGFVILLFAFTIKALREEEFLAQEFGEEFERHRRHTGLFLPRLTRARS"
     gene            7874..8632
                     /locus_tag="VFR25_07110"
     CDS             7874..8632
                     /locus_tag="VFR25_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940854.1"
                     /translation="MTRDDDRVLTKVPQITLVFWIIKIAATTLGETGGDMVSMSMNLG
                     YLAASGIFLVLFLALVGAQIVAKRFHPVLYWATIVATTTLGTTLADFATRSLGIGYAG
                     GSALLLVLLLGSLALWHRVLGSISVDTVVAPKTEMFYWVTIMFSQTLGTALGDWTADT
                     AGLGYKWAAIIFGSLLLLIAAAYYWTRISRTVLFWAAFVLTRPLGAVVGDFLDKPIEK
                     GGLALSRYGASAVLLGLMMLCIVFFRQRPAARAH"
     gene            8753..9598
                     /locus_tag="VFR25_07115"
     CDS             8753..9598
                     /locus_tag="VFR25_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940855.1"
                     /translation="MLRRPLHLLLALTIVSAGCDLSRGTEKLPSDQVIPGHIVFASER
                     DEAHGEIYIMRDDGAHVARLTFNSVEDGDPLLSPDGTKITFRRELNPASVFVMNVDGT
                     SQVGLAPGKRAAWSPDGTKLALVADSLAVMNADATGKHTFSVGASYVAWSPDGTKLAY
                     VSTGLSGQAVNDIYTILPNGTGALRITTDGLAKNSLAWSPDGTKLVYGAVLGVCVIPA
                     AGGSIQSPAQGKQPRWSPDGQRIVFVTNVYDGNDEIYSIRTDGSDPKNLSQDAAVDSD
                     PDWGH"
     gene            complement(9615..10445)
                     /locus_tag="VFR25_07120"
     CDS             complement(9615..10445)
                     /locus_tag="VFR25_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940856.1"
                     /translation="MDRILERLGLSRLPSPDYEGLCQLYGAWCARVSFDNVRKVVALQ
                     TGRDLPGRTAEEFFEAWLDLGAAGTCWPSSNALYALVRSAGFDVRRSAGSARDLGPIN
                     HATNRVVIDGRTWLVDSSMLTVDPLPLTDSVHIGAGQFAAEVEPEAGSHLVWVDFPPQ
                     GSLPFRLLPDPISLSFHLDGYERSREKSAFNRRLYARRNYPGEKRILVGNQRVSKTAA
                     GMDVRALTREELPEALAEDIGIDGRLVDEWLACGGLELAFEEAPPPPPIDRKPPSQRG
                     "
     gene            10544..11653
                     /locus_tag="VFR25_07125"
     CDS             10544..11653
                     /locus_tag="VFR25_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET9940857.1"
                     /translation="MADPRVDKLADLLVNYSIAVQPGQKIAIQGTSLAEPLIRSVYAE
                     ILKSGGHPFVLPTLPGLDELFYKYASDEQLQHIPPPNKLVIDSYDGLIAMMGSANTKA
                     LSNVSPAKMALNQRAMSEVTRTFMRRHASKELRWVGTLFPTNATAQDAEMGLTEFEDF
                     VYGACLPDLADPIGYWKKFSKWQQGLVEWLKGKKEVHVMGPDTDLRLSIAGRPFINCD
                     GRANMPDGEVFTSPVEGSVEGHVRYTYPAVYRGREVTNVRLKLEKGRVVEAKADKGQD
                     FLLRTLDVDEGARVPGEFAIGTNRGVTRFMRQTLFDEKIAGTFHMALGASIPESGGTN
                     LSAVHWDMVCDLRPGGEIRVDGELLHKNGEFVVKF"
     gene            11664..>12422
                     /locus_tag="VFR25_07130"
     CDS             11664..>12422
                     /locus_tag="VFR25_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HET9940858.1"
                     /translation="MSRPAFQNAIVRPPAPNFGDGLTTADLGSPDHALALAQHEAYCT
                     ALERCGVTLTRLPADDRHPDSTFVEDTAILAGGRAIVTRPGAVSRQGEVAAIQEALAT
                     FYDHLESIESPGTLDGGDVCEADGHAIIKLSGRTNEDGARQLAAFYAMAGMTSDTIDV
                     RVIPGILHLKSGLAYVGHGRMVAIAALASHPALSRYQVLTVDPDEEYAANCILVNDRI
                     FVPAKHPRTRAALEHLGTEVVELDMSEFQKMDGGL"
BASE COUNT         2317 a         4081 c         4077 g         1947 t
ORIGIN      
        1 tagccggccc gtgtagccga acacgcccgc gtactcgccg gccaggcttc caaacacgac
       61 gatcgcaacg gcggccaaga gtccgtaggc aagccactgc tgccctttcg gctcgcgccg
      121 cgcgatcatc ggcgcgatga agatcccagc ggccagatag gacgcggcca cccagaaaat
      181 cgccaactgg aggtgccagg ttcgcgccag gttgaagggc aggatccggg ccaggtccac
      241 tccgaagaaa gaagagatct ccgcgcggta atgctgcacg gatccaccga gcaacgattg
      301 caagaggaag agcacggcca tcacgaggaa gtaccagatc gtagctcgtt gagcaggaga
      361 cagcgcgacc tcccccggcg cccggaagga aagccgttcc tgctcgcggc cgtgccaacc
      421 gaggacgcgc catcggccga agacggccat catcgcgccg atgcccccga ggagcgcgat
      481 caaggagagc acgctccaga ccaccacgtc ggcggtcgga cggttctcga cgagaggctc
      541 gggcggccag ttgttcgtgt aggtgtggtc ctgacccggc cggagtgtcg acgcggtcca
      601 cgcggtccag gcgaagtagg ccgtgagccg gcgaaggtcc tcagggtccg tgatggcgcc
      661 gggacgaaga ccgaagcgcg tcgtcggctg cgagaacacc tcggtgtagt ggcgggtcag
      721 ctcgtcgaac gcggtggcct gctctgcggt gaagcggagc actccggatg ccggatcgta
      781 cgtgttcgtc ttgaaggagc gaacggtctg ttcgcgggcg cgatcggatc ccccgccttc
      841 gtaggaatcg cgaactatgg tggcggagcg gcgaagatag tccgccgtat agtcgggacc
      901 caggtaggcg ccgtgtccgt agatggaccc atactccatg agtccgttct tgaggaagac
      961 ctgctgtccc ccgatgacgt ccgcggcggt gaataccacg cgaccatccg gcgccacgac
     1021 ctgcttcggg atcggtggct cctcctggta cgtgcgcagc gcgagaaagc cgaggacgaa
     1081 gaagccgaag agagaaacga ggatcgcagc ctggaaccac ccgcgggcga tgaggagcgg
     1141 tcgcatgtcg cctccttggc gtgcacttgg gatccctggg agactgggag ggttaccggg
     1201 tgggggttac caagtcaagg ccggctagga ggccttccgc tcccagcaga ggtgcttcgc
     1261 gttgtccgtc ccgccctcga tcacgggctt gaagtcgaag cctgggaagc gccgctccac
     1321 ctcgttccag aacttgtcca accccgtcgc ctcggaagga aacgagcacc cgccggattc
     1381 actctcgagg aggaaccact cgtcgggaac gcggggcccg gcatcggtcg tgatgtacca
     1441 gattcgattc acgtccttcc agagtatcga ttcccgcttt cgaacggagt gctcgcacgc
     1501 gatctcgtct tcggtgatca ccagatggaa acgatcgaag gtggcccgat atttggcgag
     1561 cgcgggctcg aagatcgcga ccaggagaca gagcccgaag aagccggcca tcgcccaacc
     1621 gatccgctcg ccgtcgacga tcatccagat gccgatccca acgaacacga tcgacaccgc
     1681 ggcggtgaac cacctcatcg cgggattggg ctcatcgcat cacgcccgag cggaagacct
     1741 ccatcatccg ccccacgagt actgcaggga ggctccgtac gagtcgaagt cggctccgat
     1801 gcgcgtcgtg agcccactcg tatatacgat cttcatggag aacgaccgcc cgaacggata
     1861 ggcgaacgtc gcgccaagcc gctcatttgt ctggatctcg ctggccggac cgtcgtcgac
     1921 cgtcgtttct cctccggtcg ccataccgta gttgacggca gcccacatcc ctcgccggaa
     1981 gaggtacatc acatcccctt tcacgaacag gaggggcgac tgctcgagcg tgctgccgcc
     2041 gtagtactcg tggttgtcgg tgaagagcca agcccccgag ctcagctcga ggaaccaccg
     2101 gccctgggcg cgtgagagtc ccacctcggg ccggaacgac catcggttgg agccgagatt
     2161 gatcagccgt tccggatcgt agcggccgag gggcgcgacg acctgaacgc tcgcgcccac
     2221 gatcgttcct tggcggtagc tcgcgaactc cgcccgggag agcgcgggcg cgccgatgac
     2281 gttgagaagc acgcgaacgc gcggatccgc tggtccggtg ggctcgcggg ttcggagcac
     2341 gcccgcgaca tagccttcga agtttccgaa cccaatcgga acctgcacgt cgaccttcgc
     2401 ggttttcccg aacatgccga acgaccgaat aaggcccaag ttcacgttgt ggatcttggc
     2461 gtgcgctccc tcgatgggga gggacgaatc gaggaggatg ttgcccgtcg aataggagta
     2521 gccggcgatg gccgcgttca agccggtagg cgagctgtga tagagacccg gctccagctc
     2581 ctgcgcgatg acgggagccg ccaccaggca gaaagccagg gccgtcgcgg ccgcgatcgt
     2641 tcgggcgatc ccgactctca cccgaggatg tggagcgcgc tcagcatcgc gatcgcgact
     2701 cccatcaagc tctccccggc gatgatcccc gatgcgacgg ggaccgtgta gagcccggac
     2761 gtcttcggtt tccacttcgt gaagccgagc gcgaggagcg cgccgaggaa catcgacacg
     2821 ctgttgaatc cgttgatcgt gaacgcgagg ccgagcccta ccggcgacgg gatgaacttc
     2881 ttgagccgcg gcagccaggt ctcgagcagc gtgagcagga ttccgagcga ggccccgatc
     2941 accaggccgt agcgcgccga taacggaagc gatcccggtc ccttcgcgag gagctccgcc
     3001 acgccgcgcc acacgagcgc cgccggcgcg ggccacttct ccgttccgag gagcgcggga
     3061 tccgggatga ggatgaagaa gaccggaatc acgaccagcg cccccgcgag gacgccgaaa
     3121 aactgggcga agaactgctg gcgcggattg gcgcccagaa tgtagccgct cttgagatcc
     3181 gtgaggagat cgcccgcgct ccccacggcg ccggccgtga tgttggccgt catgaggttc
     3241 gtggggacgt tccccggcgc gatcgcgccg aaggtgagct gcgtgatctt gctcaagggg
     3301 ccgacgggcg tgatgtcggt ctccccggtc gctcgtgccg cgaccacgac caggaagaag
     3361 gtcgccagaa ccgcgatcaa gcccatccac cagtggatgt gaaagaacag atttcccagc
     3421 acgatcgcga ccgtcccgag gagcgcgtag ccgccgatga accacgagcc cggcacctcg
     3481 atccggtcca tcgggtcggc ctcgccgccg ctcttccgga agaaggcgcc gatggtgctg
     3541 aacgcgcgca cgacgctctt ccagttcatg aagaagagga ggagcccgga cgtgaccagc
     3601 atcggcacgc cggtccagag gctccaccgc gagatgttcc ggaagctggg gctcgtgatg
     3661 tcccctgtgg cgaggaagat gggcgcgagc cagacgtagt tgatcacggc gccgagcagg
     3721 aggctccagg ccgtgcggaa ggacatgagc gcgcccgccg cgatgaagag gagcgagccc
     3781 tcgaacgaca tggtcacctg attgaggagg agcggctggc cctgataggt gcccactcgc
     3841 gcccagctcg tcccccacac cgattcgatg cgcggatagc gaagccaggg cacgctcgga
     3901 aacgtgaccc aggacttgat gagagaccag tccggaatgc cccgaatgga cacatcgcgc
     3961 atccagggag cggtggcgtc gcgcatccac gtgatcaccg cgccgattcc gagcgcggag
     4021 aagagcgcgc gcgcctggcg tgccgcgtcg gctccgtgac cgtgcaggct ccgcaacgtc
     4081 tcggcggcag ccgtcccgct cgggaacttg agctgctcga cgttgatgag ctggcgcttg
     4141 gtgggcacgg ccaggaacac gccgagccaa gccaccacgg cgagccaagg gatcaggatg
     4201 gcgcatcgtg cgccgaagcc cgtcgggagc acgcccggat tgagcatgag gagcgcggga
     4261 atcgcgttga cgagcccggc tcccgtgatc gctccggccg ccgacgcgca ggactgcatc
     4321 gcgttgttct cgaggatgct gtagggcggg aagagcttcg gaagcatcgc gtggagggcg
     4381 gcgaagatgg accacgccat gatgcaggct gtgatcgcga cgccgaccgc ccatcccgac
     4441 ttcagtccga cgtacacgtt ggagcaggcc atgatcatgc cgagcagcat gcccatgacg
     4501 agcgcgcgca cggtgagctg gcgcatgcgg tcgcccgcat agacgttctc gaaccattcg
     4561 cgctcgatct gctcaggggt actgggtcgc tcggctcccg cttggggccg ttcaggtgcc
     4621 gcggtcgtgg tgctcacgag gctctcctgg ggtggggttt tgccggcggg gtcatgagga
     4681 cggtagcacc ggaggccgca cggggctaga ccttcgaggc cacgagcgcc aagaacgcct
     4741 ccggctcctc cagcggcatc atgtggccga ccttcggctg gatcgagaac gaagcggcga
     4801 ggcgggcgaa gtcgtccggc acgccccacc ccgcgcgcga tcgctcgccc gcgatcaggt
     4861 gcaccgggac ccccgagagg agcacactgt ccatcgtcgg cagatagtcg ggtgaagcgg
     4921 tggcttcgac cactgcgcgt gcgagcgcct gcacggatcg agcgggctgc gcgtcgaaca
     4981 ttcgcttcgc ccactcctgg cgcttgggct tgatctggat cttctgccgg gtcagccagc
     5041 cgtcgacgtc gctcttgaat ccatcgagca tcgcctcggc ttcctcgctc gtcatcgccg
     5101 cgatcttggt cgaccagaac gcgtcggccg gccggaaatt tccttcgacg ttcacgatcg
     5161 agacgacgcg atctcgatgg ttcgcggcga ggagcatcgc caccgcgcca ccgacggagt
     5221 gacccaccac gatggcgggc gaggcgccca gccgatcgag ctcggacgca acgtggtcgg
     5281 cgtagccgga aaggttggat ggcgccgcct tctcgacctg gccaccgtag cccggaaggt
     5341 cgagcaggat gagcttcctc gacgagggga atgggatgtc gccgaggtgg gcgaacccct
     5401 ggtagaggcc gtgaatgaag acgactggct cgggtccggt ggaggtcacg ccgcgaaccc
     5461 tatacgaact cggctcagtg cgcgatctcc cagatatgtc cgcccggatc gcggaagctg
     5521 gccgtgcgaa tgccccaggg acggtccatg ggcccgttca agagctccac ccctcgccgc
     5581 ttcagctcgg cgcacatcgc gtccacatcc tcgacctcga tcgtgaactg gaatcggacg
     5641 ccggcgtcct tccgtgcgac cgctgccggc tcgaccagcc ctggagcctg cgcaatctcg
     5701 agcaggttga cgagcgtgga cccgaagcgg aagaccgccg agtcctcggt ctcgaagacc
     5761 accggcaagc caaacgcctc cgagtagaat ttcctcgcgg ccgcgagatc ctccacgaac
     5821 aacgtgatcg cgtggattcc cttcggccat ggcccaccgg cctggttcat gacgattccc
     5881 ccttcgcctt ccctactggt cgtgcgtcgc gatctcggtg ctcttccagt tgtatttctc
     5941 cttagcgcgc ggcagccatc ccggcagcgg catcgccaga tagtcggaga gcgcctcgag
     6001 atagggcgtg tacaggtgcc gcagctcgag gaaccgcttg taagcgtccg gctcctccat
     6061 cagcggcact cccgatgatc cgagccatgc gcacatctca tggaaacgct ccgggctaca
     6121 ccgcacacca tccatgtcgc gcggtttgag ccggaacacc tgcgacacgt ccacgagcgt
     6181 gtggcgtccg atcgcgaacg tgagccgcgc ctggaacggg tcgatccccc ggaccgtggt
     6241 cgcggtgagg gcgcaagcat ccagcacggc gcacacggac gccagccagg attgattgtc
     6301 gtgctgggac cggaagaagg ccagtgtcgg ataggacaga tggctctcga ggatctccgc
     6361 cgacgcctgc tcccagtcgc ggaacagttg atcgagagcg gcattcgggt cgcggctctc
     6421 gaaggcgcgg cgcaggatga gggccgccgc gggaggcgag ccggcccact cgtccagcat
     6481 cgtgatgcgc gcttcccgcc gcgcgaactg ctggtacagg acgggaacgt acgagatcac
     6541 catggcgagg aatccaaagc cgagccccgc ctcgatgacc gtgaggacgc gtgccggcgg
     6601 gctcatgggg atcacgtcgc ccaggcccag ggtgaagaag gtggtgccgc tcaagtagag
     6661 gtctgctggg aatccctcga tgccggtcgg agcctggagg tgggagccga tcgcccattg
     6721 catgagggcg aacccggtca agagcgcggc cgcccacacg ctcaggagaa ggatcaacga
     6781 gagctgcgcg taaaagctga ggaagagctc gcgactcttc gacgcgccga accggcggct
     6841 caacgcacgc caggagagcc agagcgtccg tagaacgagc ctcgagatgc ggatgtcccc
     6901 cggcagacgt cgcggcaaga ggatcgtctc gtacgcgtcc cagaggatga tcacgacgag
     6961 cacgagaccc gccgagccgg cgatccaggt cacgagcggg ctcgcgtcag ccgtggaagg
     7021 aataggccgg tgtggcggcg atggcgctcg aactcctcgc cgaactcctg ggcgagaaac
     7081 tcctcctcgc gcagcgcctt gatggtgaac gcgaacagga ggatgacgaa ccccacgatc
     7141 gcccggatct cgccgatggc gagcacggcg ccgaggaccg ccaggagaag gccggtgtag
     7201 atcggatgcc ggatgtgcgc gtaggggccg gatcggatca aatggtgatc ctcacgaatc
     7261 acgacctcgg cgctccagtt tcggcccagg tacacgcgcg cgaagacggc cagacccaac
     7321 ccgagaatcg tgagaaggac cgcgatccac tgcacgatcg cgctatccgg aaggaagcgc
     7381 cgattgagcc cggcccatcg acggtccgcg gcgtagagga gtgcgaatcc aatccccatc
     7441 acggcgagat ggcccagacg ctcgagaacc ggttggcggc gcgccactcg acggcgaccg
     7501 atggccgcca ccgcccagta gattccccac aggaaccaga gcgcgaagat caccgtgcga
     7561 gagtcgatcg atccctccgg gagtgtgctc gggcggagcc aacgatgtgc cggcaaggaa
     7621 tgtagaccgg catcgaccac gctacaagcc gccgggggtg cgagaagagg catcccgagc
     7681 cctacgcgga gcctgaatcc aagacgatgc atgaccttcc ggcctcgcta caatgcgctg
     7741 aatggtcccg gtcgaggcct ccacgcaggc ccgtagattc cctgtaaatt ctgggatttc
     7801 gctctgagga acggcacaca tgctgcttct ggataacgtg acgccagctc gcacctgcac
     7861 cgaggagaca tccatgacca gggatgacga tcgcgttctg accaaggtcc cccagatcac
     7921 actcgtcttc tggatcatca agatcgcggc gacgacgctc ggagaaacgg gcggcgacat
     7981 ggtgtcgatg tcgatgaacc tgggctatct cgcggcgagt gggatcttcc tggtcctctt
     8041 cctcgcgctc gtaggggccc agatcgtcgc gaagcgattc catccggttc tctactgggc
     8101 cacgatcgtc gcaacgacca cactgggcac gacgctcgcg gacttcgcca cacgctccct
     8161 cgggatcggc tacgcgggcg gctcggcgct cctcctcgtt ctgctgttgg gctcgctcgc
     8221 actgtggcat cgcgtgctcg gctcgatctc cgtcgacacc gtggtcgcgc ccaagaccga
     8281 gatgttctac tgggtgacga tcatgttctc gcagacgctg ggaaccgcgc tcggggactg
     8341 gaccgcggac accgcggggc tcggctacaa gtgggccgcc atcatcttcg gatcgcttct
     8401 gctcctgatc gcggcggcgt actactggac gaggatctcg cgcaccgtgc tcttctgggc
     8461 cgctttcgtg ctcacgcgtc cgttgggggc cgtggtggga gacttcctcg acaagccgat
     8521 cgagaagggc gggctggcac tgagccggta cggcgcctcg gccgtcctgc tcgggctcat
     8581 gatgctgtgc atcgtgttct tccggcagag gcctgcggct cgcgctcatt agtcgcaaac
     8641 tgcctattta caataattta ctaggctaat ctcacctagc gcatctgccg atacttccgg
     8701 ggaatcccct ctcgtcaggg actggactcg aaccccggca aaggatcggc ccgtgcttcg
     8761 tcgaccgctc caccttctcc tcgctttgac gatcgtatcc gccggctgcg acttgagccg
     8821 cggcacggaa aagctcccct ccgaccaggt catccccggg cacatcgtct tcgcgagcga
     8881 acgcgacgag gcgcatggcg agatctacat catgcgggac gacggcgcac atgtggcgcg
     8941 gctcacgttc aacagcgtgg aggacggcga tccgctcctc tcccccgacg gaaccaagat
     9001 cacgttccgg cgcgagctca atcccgcgtc ggtgttcgtg atgaacgtgg acggcacgtc
     9061 ccaggtcgga cttgcgcccg gcaagcgcgc ggcctggtcg ccggacggca ccaagctcgc
     9121 gctggtggcg gacagcctcg ctgtcatgaa tgccgatgcg accggcaagc acaccttcag
     9181 cgtgggcgca tcctacgtgg catggagccc ggacggaacc aagctcgcct acgtgtcgac
     9241 gggcctcagc ggccaggccg tgaatgacat ctacacgatc ctgccgaacg ggaccggagc
     9301 tctgaggatc acgaccgatg gcctcgcgaa gaactccctc gcgtggtcgc ccgatgggac
     9361 caagctcgtc tatggggcgg tgctgggagt ctgcgtgatt ccagcggcgg gcggcagcat
     9421 ccagagtccg gcacagggca agcagccgcg ctggtccccg gacggtcagc ggatcgtctt
     9481 cgtgacgaac gtgtacgacg gaaacgacga gatctactcg atccgcaccg acggaagcga
     9541 tccaaagaac ctgagccagg acgccgctgt ggactccgat cccgactggg ggcactgaga
     9601 acccccggcg cggatcagcc gcgctgtgac ggcggcttcc ggtcgatagg aggaggagga
     9661 ggcgcctcct cgaacgcgag ctcgaggcca ccacacgcga gccattcgtc caccaaccgg
     9721 ccgtcgatcc cgatgtcctc cgcgagcgcc tcgggcagct cttcccgcgt cagcgcgcgc
     9781 acgtccatcc cagccgccgt cttcgacacg cgctggttcc ccaccagaat tcgcttctcg
     9841 cccggatagt ttcgtcgcgc gtagagccgc cggttgaacg cgctcttctc gcgcgaccgc
     9901 tcgtagccgt ccagatggaa cgagagtgag atcggatcgg gcaggagccg gaacggaagc
     9961 gagccctgcg gcgggaaatc gacccacacg agatggcttc ccgcctccgg ctcgacctcg
    10021 gccgcgaact gcccggcccc gatgtgaacg ctgtcggtga gcggaagggg atcgacggtg
    10081 agcatggagc tgtccacgag ccaggtccgg ccgtcgatca ccacgcgatt ggtcgcgtga
    10141 ttgatcggac ccaggtcgcg tgcggatcct gcgctccgcc tcacgtcgaa accggcgctc
    10201 cggaccagag cgtagagggc gttgctgctc ggccagcagg ttcccgccgc gccgagatcg
    10261 agccaggcct cgaaaaactc ctccgcggtc cgccccggca ggtcccggcc ggtctgaagc
    10321 gcgaccacct tgcgcacgtt gtcgaacgag acgcgcgcgc accaggcgcc atagagctgg
    10381 cacaggccct cgtagtcggg cgacggcaat cgggagagtc ccagccgctc gaggatacgg
    10441 tccaagagag gcgcgggaag ggccggttcc tccagagtgg tcgcggaaat ctcgcccatg
    10501 cggacactgt accatctccc agcattggtg tagcttcacc gccatggccg atccgcgcgt
    10561 cgacaagctg gccgacctcc tcgtgaacta ctccatcgcg gtccagcccg gacagaagat
    10621 cgcgatccag gggacgtcgc tcgccgagcc cttgatccgg tccgtctatg ccgaaatctt
    10681 gaagtccggg gggcatccct tcgtgctccc gaccctgccc ggcctggacg agctcttcta
    10741 caagtacgca tcggacgagc agctccagca catcccgcct cccaacaagc tcgtgatcga
    10801 ttcctacgac gggctcatcg cgatgatggg gtccgcgaac accaaggcgc tcagcaacgt
    10861 ctcacccgcg aagatggcgc tgaatcagcg cgccatgagc gaggtcacgc gcacgttcat
    10921 gcggcgccac gcgtcgaagg agctccgctg ggtcgggacg ctcttcccga cgaacgcgac
    10981 ggctcaggac gccgagatgg gcctcaccga attcgaggac ttcgtctacg gcgcctgcct
    11041 ccccgatctc gcggacccga tcggctactg gaagaaattc tcgaagtggc agcagggcct
    11101 cgtggaatgg ctcaagggga agaaggaagt ccacgtgatg gggcccgaca ccgacctccg
    11161 cctgagcatc gcggggcgtc cgttcatcaa ctgcgacggc cgcgcgaaca tgcccgacgg
    11221 cgaagtcttc acgagcccgg tggagggctc cgtcgagggg cacgtacgct acacgtaccc
    11281 cgcggtctac cgcggacgcg aggtgacgaa cgtgcggctc aagctcgaga aggggcgagt
    11341 ggtcgaagcc aaggcggaca aggggcagga cttcctgctc cgcacgctcg acgtggacga
    11401 gggagcgcgc gtgcccggcg agttcgcgat cggcaccaat cgcggcgtca cgcgattcat
    11461 gcgccagacc ctgttcgatg aaaagatcgc cgggacgttc cacatggcgc tcggcgccag
    11521 catccccgag tcgggcggca ccaacctttc cgcggtgcac tgggacatgg tctgcgatct
    11581 caggcccggc ggtgagatcc gggtcgacgg cgagctcctg cacaagaacg gagagttcgt
    11641 cgtcaagttc tgagcgacgg atggtgagcc ggccagcatt ccaaaatgcc atcgttcgac
    11701 cgccggcccc gaacttcggc gacggcctca cgacagccga tctaggatcc cccgaccacg
    11761 cactcgcgct ggctcagcac gaggcctatt gcacagcgct cgagcgctgc ggggtcacgc
    11821 tgacgcggct ccccgccgat gaccgccacc ccgactccac gttcgtcgag gacaccgcga
    11881 ttctcgcagg cggtcgcgcg atcgtgacgc ggcctggcgc ggtgagccgt caaggggagg
    11941 tcgcggccat ccaagaggcg ctcgcgacgt tctacgacca cctcgagtcg atcgaaagcc
    12001 ccggaaccct ggatggcggc gatgtgtgtg aagccgatgg gcacgcgatc atcaaactct
    12061 ctggtcgaac gaatgaggac ggagcgcggc agctcgcggc cttctacgcc atggcgggga
    12121 tgacgtccga taccatcgac gttcgcgtta tcccgggcat cctgcacttg aagagcggcc
    12181 tcgcctacgt tggtcacggc cgcatggtag cgatcgcggc actcgccagc catcccgctc
    12241 tctcgcgtta tcaagtgctc acggtggacc cggatgagga gtacgccgcc aactgcatcc
    12301 tcgtgaacga ccgcattttc gtgccggcca agcatccccg cacgcgcgcc gcgctcgagc
    12361 atctcggcac agaggtggtc gagctcgaca tgtcggaatt ccagaagatg gacggcgggt
    12421 tg
//
LOCUS       DASTKC010000172         5500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1797782, whole genome shotgun sequence.
ACCESSION   DASTKC010000172 DASTKC010000000
VERSION     DASTKC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797782"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFR25_07135"
     CDS             <1..576
                     /locus_tag="VFR25_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9940859.1"
                     /translation="AEAKKAAESDTTGITKAPAKPTVRIDRRKVLGPATRSVELIYLG
                     KSAHTDGDLIVYLPKQKIIFAGDLVSNRSVPWLLDPKFDRLGWIASVDSLRSKRFSSV
                     TTLVPGHGTMDKPIQEIAYTWHYLHDSWDRAAKIANDGTSLAAFKDWGFLGPYEGDEF
                     YAEVNFMNMRRLYNEAKGIKTPGRPGTRAYK"
     gene            563..1186
                     /locus_tag="VFR25_07140"
     CDS             563..1186
                     /locus_tag="VFR25_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940860.1"
                     /translation="MRTSSRRAIVGGLVAACATLAALLAGCTREAPDVHEARLAAERY
                     IDALAHKDLKEIRARSTCVASYQSLQGGNVLQIGDLHHVTVATIDSLGRAAGEAHRSA
                     DSGWGGAPDSIRDELSKRAMAIARLHFVYRSALRALSLSRPDSLLGSNAVLDTRTLRV
                     RVRYAGEAVGPRAIDKEVLLRLIRAPGGQWIAFSFYSTEDDPRPERV"
     gene            1283..2296
                     /gene="aroF"
                     /locus_tag="VFR25_07145"
     CDS             1283..2296
                     /gene="aroF"
                     /locus_tag="VFR25_07145"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9940861.1"
                     /translation="MVITLRPDATPEQRRAIESRLREAGFQIVLSPNGSAVTMAVVGE
                     GSLPPLDEVRAMPGVATAIRIPEPFKLASRTFRQETSVIRIGDVAIGGKDVVLMAGPC
                     TIESEEQMVKTAAAVRAAGARVMRGGAFKPRTSPYSFQGLGEEGLKLMRRVADAHGLL
                     VISEIMDKSQIALMDRYVDIFQVGARNMQNYPLLRELGRVERPVLVKRGLAATVDEWL
                     MSAEYVMSGGNDRVIVCERGVRAYETYTRNTLDLNAVAVAKSISHLPVIVDPSHAAGV
                     RDKVVPLARAAIATGADGLLVEVHHDPERAICDGPQSLFPQQFEALAAQIRLIAEAVG
                     RSL"
     gene            2296..3939
                     /locus_tag="VFR25_07150"
     CDS             2296..3939
                     /locus_tag="VFR25_07150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9940862.1"
                     /translation="MAAQDRGRSGRPGKDTRRKPGGRAPRSQAASPARSLIPHSWISG
                     IALGSLAVYALYWASQVFRYHTIGNYTVETDFYWKYGPAARDLLRGAVDIANYDSKGW
                     GYPAVVALLARAGMEVFQAGQLVALLSAVVAGWLAYRLHRSLLGPGVALASLGLLLVN
                     PTFVVNTYEVGTDMFFFAVVLASMALLLRSRAPKAWAIAISGLLGGWAFSTRYNGLFL
                     LAGALVYLLLIEMPRGPWGPRLQRAALWTAGFVLAALPWLWINAAHTGNPLTNTNYLN
                     VGYEVYGKGNWEQFMYGSDRKIHSLVDVVRLDPMLFLGAMLKNVFVHLKQDLTELMPI
                     LWGAFALIGGLLLATRERPERRTAAYAVFWALYFLTLVPVFYGTRFSLPLVAFYGLLA
                     AWPFVSPWLGRSIQGIEGSFPLRTFLFLALWVGALFHTYQAVGNPKNPEGLRAGPHEI
                     MDAVGYLKTHGAGEALLARKPHAAFLANMRFVPIPTVDSPAALHDVAVKERARYLLIS
                     AAEIQMRAALRSFATGEEIPGFRLVYDSQGALVYEVVPL"
     gene            3995..4071
                     /locus_tag="VFR25_07155"
     tRNA            3995..4071
                     /locus_tag="VFR25_07155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Val,seq:gac)
     gene            4098..>5500
                     /gene="thrS"
                     /locus_tag="VFR25_07160"
     CDS             4098..>5500
                     /gene="thrS"
                     /locus_tag="VFR25_07160"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9940863.1"
                     /translation="MIRIDVNGRQLEAEPGSTLGQIFHEQYQDAFKLGIAARLNGRIV
                     DYHTPVHDSGKAELIPVDTPEGLAVLRHSTAHLMASAVVKLFPGTKLAIGPAIDEGFY
                     YDFQAPRPFQPEDLEKIETSMHEIAEENHMFQRGELSRQDALQKFGAEGETFKVELIE
                     GLPDPKLSTYTHSFFTDLCRGPHVPRTKMLKHFKLLSVAGAYWRGDSNRPMLQRIYGT
                     AFPSKDRLKEHLTRLEEAKLRDHRKVGHALDLYSVQDIAGGGLIFWHPHGAAMRRVIE
                     DFWKSEHLKRGYTIVNTPHIAQADLWQRSGHTNFYRDNMYFLEIDEREYVLKPMNCPF
                     HILIYQGKKHSYRELPIRIAELGTVYRYEKSGVLHGLARVRGFTQDDAHLFCTREQVE
                     PELEKVLAFARFMLDTFGFKDYKVELSVRDPKRKEDYMGSDEDWELAEGALERVVQRA
                     GIIYRRAEGEAVFYGPK"
BASE COUNT          917 a         1908 c         1754 g          921 t
ORIGIN      
        1 gccgaagcca agaaggctgc cgagagcgat accaccggca tcacgaaagc gcccgcgaag
       61 ccgaccgttc gcatcgacag gcgcaaggtg ctcgggccgg cgacgaggag cgtcgagctg
      121 atctatctcg gcaagagcgc gcataccgac ggcgacttga tcgtctacct gcccaagcag
      181 aagatcatct tcgcgggcga cctcgtgtcg aaccgttcgg tgccgtggct cctggatccc
      241 aagttcgatc gcctcggctg gatcgcctcc gtcgactcct tgaggagcaa gcgcttctcg
      301 tccgtgacga cgctcgttcc cggtcatggc acgatggaca agcccatcca ggagatcgcc
      361 tacacgtggc actacctgca cgattcctgg gatcgcgcgg ccaagattgc caacgacggc
      421 acgagcctcg cggccttcaa ggactggggc ttccttggcc cctacgaggg ggatgagttc
      481 tacgccgagg tcaatttcat gaacatgcga cgcctttaca atgaggcgaa gggaatcaag
      541 acgccggggc gcccagggac gcgtgcgtac aagtagccgt cgcgccattg tcggcgggct
      601 tgttgcggcc tgcgccacgc tggccgcgct cctcgccggc tgcacgcgcg aagcgcccga
      661 cgtgcacgag gcccgcctgg ccgccgagcg ctacatcgac gccctggcgc acaaggacct
      721 caaggagatc cgcgcccgct ccacctgcgt cgcctcctac cagtcgctcc aagggggcaa
      781 cgttctccag atcggcgacc tgcaccacgt gacggtcgcc accatcgatt cgctcggccg
      841 cgcggcgggg gaggcgcacc gaagcgccga ctccggctgg ggcggggctc ccgattccat
      901 cagggacgaa ctctccaagc gagcgatggc gatcgcgagg ctccacttcg tctaccgaag
      961 cgcgctccgg gcgctctcgc tgtcccgacc cgactcgctc ctcggatcca acgccgtgct
     1021 cgacacgcgc acgttgcgcg tgcgcgtgcg ctacgccggc gaagccgtgg gccctcgcgc
     1081 gatcgacaag gaggtcctct tgagactcat ccgggcaccg ggcggccagt ggatcgcctt
     1141 ctcgttttac agcacggagg acgaccctcg tcccgagagg gtttgaccgc cgcccggccg
     1201 ggtgctagcc ttcggccgtg tcgcccgacc tctgcccatt gccgatggct cggcagtggg
     1261 ccttcgtgtt tctgaggacc ccatggtcat cacgctgcgg cccgatgcga ccccggagca
     1321 gcgccgcgcg atcgaatcgc gcctgcgcga ggccgggttc cagatcgtgc tctccccgaa
     1381 cggctcggcc gtaaccatgg ccgtggtcgg agaggggagt ttgccgccgc tcgacgaggt
     1441 ccgggccatg cccggcgtcg cgaccgccat ccgcattccc gagcccttca agctcgccag
     1501 ccgtacgttc cgccaggaga cgtccgtcat ccggatcggc gacgtcgcga tcggaggcaa
     1561 ggacgtcgtg ctcatggccg gcccctgcac catcgaatcc gaagaacaga tggtgaagac
     1621 cgccgccgcg gtacgcgcgg cgggcgcgcg cgtcatgcgg ggcggcgcat tcaagcctcg
     1681 cacatcgccg tactcgttcc agggactcgg cgaggagggg ctcaagctca tgcggcgcgt
     1741 cgccgacgcg cacggcctcc tcgtcatctc cgagatcatg gacaagagcc agatcgcgct
     1801 catggaccgg tacgtggaca tctttcaggt cggcgcgcgg aacatgcaga actatccgct
     1861 cctccgggag ctgggaaggg tggagcggcc cgttctcgtg aagcgcggcc tcgccgccac
     1921 cgtggacgag tggctcatga gcgcggagta cgtcatgtcc ggcgggaacg atcgcgtcat
     1981 cgtgtgcgag cgcggcgtgc gcgcctacga gacctacacg cgaaacacgc tcgacctgaa
     2041 cgccgtcgcg gtggcgaagt cgatcagcca tctgcctgtg atcgtggatc cgagccacgc
     2101 ggccggcgtg cgggacaagg tggtgccgct cgcgcgcgcc gccatcgcga ccggcgcgga
     2161 cggcctcctg gtcgaggtcc accacgatcc ggagcgggcg atctgcgacg gtcctcagtc
     2221 cctcttcccg cagcagttcg aggccctcgc ggcgcagatc cgtctgatcg ccgaggcggt
     2281 cggccgctcg ctctgatggc cgcccaagac cgcgggcgct cggggcgtcc ggggaaggac
     2341 acccgccgga agcccggcgg acgcgccccg cggagccaag ccgcaagccc cgcgcgctcc
     2401 ctgattccac attcctggat ctcgggaatc gcgctcgggt cgctcgccgt ctacgcgctc
     2461 tactgggcga gccaggtctt ccgctaccac acgatcggaa actacaccgt cgagaccgat
     2521 ttctactgga agtacgggcc ggccgcgcgc gatcttctcc gcggagccgt cgacatcgcg
     2581 aactacgatt cgaagggctg gggctacccg gccgtcgtcg cgctcctcgc gcgagccggg
     2641 atggaggtct tccaggcggg gcagctggtc gcgctcctct ccgcggtcgt ggccggatgg
     2701 ctcgcgtacc gcctccaccg aagcctcctc ggacccgggg tcgcactcgc gagcctcgga
     2761 ctcctgctcg tgaatcccac gttcgtcgtg aacacgtacg aagtgggaac cgacatgttc
     2821 ttcttcgcgg tggtgctcgc ctccatggcg ctcctgctcc gaagccgcgc ccccaaggcg
     2881 tgggcgatcg cgatctccgg gctactcggc ggctgggctt tctcaacgcg ctacaacggg
     2941 ctctttctcc tggccggcgc tctcgtctac ctgctcctga tcgagatgcc gcgcggtccc
     3001 tggggaccgc ggctccagcg cgcggcgttg tggacggcgg gtttcgttct cgcggcgctg
     3061 ccctggctct ggatcaatgc cgcgcacacg gggaatcccc tcacgaacac gaactacctg
     3121 aacgtcggct acgaggtgta cgggaagggg aactgggagc agttcatgta cgggagcgat
     3181 cggaagatcc actcccttgt cgatgtcgtg cggctcgacc ccatgctgtt cctcggcgcg
     3241 atgctcaaga acgtattcgt gcacctgaag caggatctga ccgagctcat gcccatccta
     3301 tggggagcct tcgccctgat cggaggtctc cttctcgcga cgcgcgagcg gccggagcga
     3361 cggacggcgg cgtacgcggt cttctgggcg ctctacttcc tcacgctcgt cccggtcttc
     3421 tacggaaccc gcttctcgct cccgctcgtc gccttttacg gcctcctcgc cgcatggccc
     3481 tttgtctccc cgtggctcgg ccgctccatc cagggaatcg aagggtcgtt cccgctccgg
     3541 accttcctct tcctcgcgct ctgggttgga gcgctcttcc acacgtatca agccgtggga
     3601 aatcccaaga acccggaagg gctgcgcgcg gggccgcacg agatcatgga cgcggtgggc
     3661 tatctcaaga cacacggagc gggagaagcg ttgctcgcga ggaagccgca cgccgccttc
     3721 ctcgcgaaca tgcgcttcgt cccgatcccg acggtcgatt cgccggccgc gctccacgac
     3781 gtcgccgtga aggagcgcgc gcggtatctc ctcatctcgg ccgcggaaat ccagatgcgc
     3841 gccgcgctcc gatcgttcgc gacgggggag gagatcccgg gattccgcct cgtgtacgac
     3901 agccagggag cgctcgtcta cgaggtcgtg ccgctctagc cgagacttta cttcgcagtc
     3961 ggttctgcta cactcgccgc tcgcatttgg taacgggcga ttagctcagc tggttagagt
     4021 acctgcttga catgcagggg gtcagtggtt caagtccact atcgcccacc atcttcatac
     4081 gaaggacatt cccgaccgtg attcgtatcg acgtcaatgg ccgtcagctc gaggcggagc
     4141 ctggctccac cctcggccag atcttccacg agcagtacca ggacgcgttc aagctgggca
     4201 tcgcggccag gctgaacggc cgcatcgtgg actaccacac gccggtccac gattccggca
     4261 aggcagagct catcccggtg gacacgccgg agggtctcgc cgtgctccgc cactcgacgg
     4321 cgcacctcat ggcctcggcc gtggtgaagc tcttcccggg caccaagctc gcgatcggcc
     4381 ccgccatcga cgaaggcttc tactacgact tccaggcgcc ccggccgttc cagcccgagg
     4441 atctcgagaa gatcgagacc tcgatgcacg agatcgccga ggagaaccac atgttccagc
     4501 gcggcgagct gtcgcgccag gacgcgctcc agaagttcgg agcggaaggg gagaccttca
     4561 aggtcgagct gatcgaggga ttgcccgatc ccaagctcag cacgtacacc cattccttct
     4621 tcaccgatct ctgccgcggg ccccacgtgc cgcgcacgaa gatgctgaag catttcaagc
     4681 tcctctcggt ggccggcgcc tactggcgcg gcgactcgaa ccgcccaatg ctccagcgga
     4741 tctacggcac cgcgttcccg agcaaggacc ggctgaagga gcacctgacc cggctggagg
     4801 aagcaaagct ccgcgatcat cgcaaggtgg ggcacgcgct cgacctctac agcgtgcagg
     4861 acatcgccgg gggcggactc atcttctggc acccgcacgg cgccgccatg cgccgcgtga
     4921 tcgaggactt ctggaagtcg gagcacttga agcgcggcta caccatcgtg aacacgccgc
     4981 acatcgcgca ggcggacctc tggcagcgct cggggcacac caatttctac cgcgacaaca
     5041 tgtacttcct cgagatcgac gagagggagt acgtgctcaa gccgatgaac tgccccttcc
     5101 acatcctcat ctaccagggg aagaagcact cgtaccgcga gctgcccatc cggatcgcgg
     5161 agctgggcac ggtctatcgc tacgagaagt cgggcgtgct ccacggcctc gcccgcgtcc
     5221 gcggattcac ccaggacgac gcgcatctct tctgcacccg ggagcaggtg gagccggagc
     5281 tcgagaaggt gctcgcgttc gcccgattca tgctcgatac gttcggcttc aaggactaca
     5341 aggtcgagct ctccgtccgc gatcccaagc ggaaggaaga ctacatgggc agcgacgagg
     5401 actgggagct ggccgaggga gcgctcgagc gggtcgtcca gcgcgcgggg atcatctatc
     5461 gccgcgccga gggagaggct gtcttctacg gccccaagat
//
LOCUS       DASTKC010000173         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2035620, whole genome shotgun sequence.
ACCESSION   DASTKC010000173 DASTKC010000000
VERSION     DASTKC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035620"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFR25_07165"
     CDS             complement(<1..400)
                     /locus_tag="VFR25_07165"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9940864.1"
                     /translation="MVRKGSAEWKGSLKEGKGTVSTESGGLSNHPHSFHTRFGEERGT
                     NPEELIAAAHAGCFSMALSGKLGAEGLTAESIRTTAAVTLDKVEGGFAITAVHLDVVA
                     RVPGADRAKFEKAAQDAKSGCPVSKLLNARI"
     gene            complement(467..2149)
                     /locus_tag="VFR25_07170"
     CDS             complement(467..2149)
                     /locus_tag="VFR25_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HET9940865.1"
                     /translation="MVRSLAALAALALIALWSGPALAANFILINLDGPGEGFNDPTPV
                     APIGGNEGTTRGEQRLKVFETAGAIWGTYLQSNVDIVVEAQFDALTPCDTTGGVLGSA
                     GAVTVASDFTNAPIANTWYSIALANKLAGADQSLGVSDISARFNSSVDNATCLGSVSW
                     YYGLDHNEGQDEDLLAVVLHELGHGLGFQTLTTLSTGQFFSNRPDIFARNLFDITQNK
                     TWDAMTNIQRRNSAINTGNLVWNGASVKGHTSLFLGPVSIVHVDSPASIAGNKAFGLA
                     DFGPQPGVPVLTAPVVLVNDGTGTTSDACETILNGAAIAGSIALIDRGTCTFVSKAKA
                     AQDAGAVGVIIGNNVAGAPPGMSGVDPTVTIPTVSISQADATAIKGQLGVGVVATFGT
                     DPSHLAGADATGRPRLYTPNPIESGSSVSHFDTSLQPNALMEPFINSDLTSSPDLTQY
                     AFVDEGWYATATAVAEGPGAAPRAYAAPNPFASATTMNFALKSAGKTTIEVFDVRGAL
                     VKALPTAWRAAGFQHATWDGTDAVGHRVAGGVYYWRARSGDFEAGGRLVRLQ"
     gene            complement(2151..2645)
                     /locus_tag="VFR25_07175"
     CDS             complement(2151..2645)
                     /locus_tag="VFR25_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940866.1"
                     /translation="MPVPRTRRFRHRSAPILATLVLLIVAPTTAHADTPAWSFDWRPG
                     WVPGDPVEESSFDFWTMPSNPGLFVAIDPVTHRPVAPSPEQRAAFQRAFDLNELLAPS
                     APPRIEALPHGGEIAHLNGAFQSFSIARRDASGRYVNDCAPDAKTALQLLSTPVSSAP
                     KEER"
     gene            2838..4250
                     /locus_tag="VFR25_07180"
     CDS             2838..4250
                     /locus_tag="VFR25_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9940867.1"
                     /translation="MPEIQLLEQRLGSLETESLKLDATPDEIQRWLEALSRFSAEYLE
                     ANTAAPAFLPLGPPHLAQPLPLRPTPLPDVLEEFKGALGRGIVATSGRFFGYVPGGGI
                     PTGAVGDYLAALTNRYSGNYGAAPGAAAIENDAVRWLIEMVGYPETAWGTLQSGGSLA
                     TLTAVVAARESRQAWDWMRSVIYMTEECHLAIRKSLHIAGLAHVACRTVPVDERLHMS
                     VPDLERMIAEDRSKNLQPWMVFASAGTVNTGAIDPLGEIAKVAKDEGLWMHVDGAYGA
                     FFVLAERARASLEAMALADSVVLDPHKGLFLPYGCGAVLVKNGEVLRQAFAATHNYLS
                     DVEHEERSPSSYSPELTRHFRALRLWMSLKVHGIERFRSALEEKLLLAQLAWQRLRAM
                     PRIDPGPEPELSCVAFRVRDEGDGPTREMLDRILARGRVHMSSTVLGGKLYIRICVLC
                     FRSHRADLDEAISEIERIAS"
     gene            4299..>4759
                     /locus_tag="VFR25_07185"
     CDS             4299..>4759
                     /locus_tag="VFR25_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011716883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9940868.1"
                     /translation="MQSFAWRAGTLSLLAALLLSGSALAKTAPPSPHAPSFTLPTANG
                     SVCLDSLRGQAVLVDFWASWCGPCKQSFPWMTALHEKYATKGLTIVAINLDKDRKLAD
                     AFLEQHPAPFVVAFDPSGKTAKAFKVWGMPSSFLVGADGTILFSRAGFDPK"
BASE COUNT          770 a         1566 c         1631 g          792 t
ORIGIN      
        1 tgatccgcgc gttcaggagc ttcgagaccg gacagcccga cttggcgtcc tgcgcggctt
       61 tctcgaactt cgcgcgatcg gcgccaggaa cgcgggcgac cacgtccagg tggaccgcgg
      121 tgatggcgaa gccgccctcg accttgtcga gcgtgaccgc ggccgtggtg cggatggatt
      181 cggcggtcag gccttcggcg ccgagcttgc ccgagagggc catggagaag cagcccgcgt
      241 gggccgcggc gatcagctcc tccggattgg ttccgcgctc ctccccgaag cgggtgtgga
      301 acgagtgggg atggttcgag agcccgccgc tctccgtgga gaccgtgccc ttgccttcct
      361 tgagactacc cttccattcg gcggaaccct tgcggaccat ggcgaccccc gtgtcggatg
      421 cggtgttcag tcgtgctccc gcatccatac tgacggatcg cgatggctac tgcaaacgaa
      481 cgagccgtcc gccggcctcg aagtcccccg agcgcgcgcg ccagtagtac acgccgcccg
      541 cgacgcgatg tccgaccgcg tcggtgccgt cccaggtcgc atgctggaag cctgcggcgc
      601 gccacgccgt agggagcgcc ttcacgagcg cgccgcgcac gtcgaagacc tcgatcgtgg
      661 tcttcccggc gctcttcagc gcgaagttca tggtggtcgc cgacgcgaac ggattgggcg
      721 cggcataagc gcggggcgcc gcgccaggac cctcggccac ggcggtcgcc gtggcgtacc
      781 agccctcgtc cacgaacgcg tactgtgtga gatccggcga cgacgtgaga tccgagttga
      841 tgaagggctc catgagcgcg ttcggctgga gcgaggtgtc gaaatgcgac accgaggaac
      901 cgctctcgat tggattcggc gtgtagaggc gcgggcggcc cgtggcatcc gcgccggcca
      961 gatgggacgg atccgtacca aatgtagcga caacacccac gcccagctgc cccttgatcg
     1021 cggttgcgtc ggcctgcgag atgctgaccg tggggatcgt gaccgtcgga tcgacgccgg
     1081 acatgccggg gggcgcgccg gccacattgt ttccgatgat cactccgacg gccccggcat
     1141 cctgagctgc cttggccttc gagacgaacg tgcaggtgcc ccggtcgatc agcgcgatgc
     1201 tccccgcgat ggccgctcca ttcaggatcg tctcgcaggc gtcgctcgtc gttcccgtac
     1261 cgtcgttcac gagaaccacg ggggcggtga ggacaggaac gccgggctga ggaccgaaat
     1321 cagcgagacc gaacgccttg ttccccgcaa tcgatgccgg ggagtcgacg tgcacgatcg
     1381 agaccggccc gaggaagagg ctcgtgtgac ctttgaccga cgcgccgttc cagaccaggt
     1441 ttcccgtatt gatcgcggag ttcctccgct ggatgttggt catcgcgtcc caggtcttgt
     1501 tctgggtgat gtcgaagaga ttgcgggcaa agatgtcggg acgattgctg aagaactgtc
     1561 cggtcgagag cgtggtgaga gtctggaagc cgagcccgtg ccccagctcg tggagcacga
     1621 ccgcgaggag gtcctcgtct tgtccctcgt tgtgatcgag tccgtagtac cagctgacgc
     1681 tgcccaggca ggtcgcgttg tccacgctcg agttgaagcg ggcggagata tcgctcaccc
     1741 ccagcgactg gtcggcgccc gcgagcttgt tcgcgagcgc gatcgagtac caggtgttgg
     1801 cgatcggagc gttggtgaag tcggacgcga cggtgaccgc gcccgcgctt ccgagcacgc
     1861 ccccagtcgt gtcgcacggc gtgagcgcgt cgaactgcgc ctcgacgacg atgtcgacgt
     1921 tgctctggag ataggtgccc cagatcgcgc cggcggtctc gaagaccttg agcctctgct
     1981 cgccgcgcgt ggtgccttca ttgccgccga tcggcgcgac cggcgtggga tcgttgaacc
     2041 cctcaccggg tccgtcgaga ttgatcagga tgaagttcgc ggcgagagcc gggccggacc
     2101 acagagcgat cagcgccaga gccgcgaggg ctgcgagcga gcggaccatg ctagcgctcc
     2161 tccttcggcg ccgacgacac cggcgtggag aggagttgga gcgccgtctt ggcgtccgga
     2221 gcgcagtcgt tcacgtaccg cccggaagcg tcgcggcgcg cgatcgagaa ggactggaag
     2281 gcgccgttca agtgcgcgat ctcgccgccg tggggcagcg cctcgatgcg aggcggcgcg
     2341 ctgggagcca ggagctcgtt cagatcgaag gcgcgctgga atgcggcgcg ctgctccggc
     2401 gagggagcga cggggcggtg cgtcaccgga tcgatcgcga cgaagagccc gggattggac
     2461 ggcatcgtcc agaagtcgaa cgacgactcc tcgacgggat cgccaggaac ccagccgggg
     2521 cgccagtcga acgaccacgc aggggtgtcg gcgtgtgcgg tcgtgggtgc gacgatgaga
     2581 aggacgaggg tggcgagaat gggggcggag cgatgccgga agcgtcgggt ccgggggacg
     2641 ggcatgtgga gatcctccga tgcggcgagg cgatgagatc gcggatcggg cgccccctcg
     2701 tccgatccgg catatgttgt ttcgatagtc cctaagtata gcacgcagag cgccttgagt
     2761 ccacgccgtt tggaccgcga cggcgcgctc tgctatgttg cacggaccat acggcctcgc
     2821 tccaccccgg agatttcatg cccgagatcc agctcctgga acaaaggctc ggctcgctcg
     2881 agaccgaatc cctcaagctc gacgcgacgc cggacgagat ccagcgctgg ctcgaagctc
     2941 tgtcccgatt ctccgcggag tatctcgaag cgaacacggc cgcgcccgcg ttcctgccgc
     3001 tcggcccgcc gcatctggcc caacccctcc cgctccgtcc cacgccgctc cccgatgtcc
     3061 tcgaagaatt caagggcgcg ctcgggcggg gcatcgtcgc gacgtccgga cgcttcttcg
     3121 gctacgtccc cgggggcggc attcccacgg gcgccgtcgg cgactatctc gccgcgctca
     3181 ccaatcgcta ctcaggcaac tacggcgcgg cgccaggggc ggcggcgatc gagaacgacg
     3241 cggtgcgctg gctgatcgag atggtcggct accccgagac ggcttggggc acgctccaga
     3301 gcggcggctc gctcgcaact ctcaccgccg tcgtggccgc gcgggaatcg cggcaggcgt
     3361 gggactggat gcggagcgtc atctacatga ccgaggaatg ccatctcgcc atccgcaagt
     3421 cgctccacat cgccgggctc gcgcacgtgg cgtgccggac cgtaccggtg gacgaaaggc
     3481 tgcacatgtc cgtgcccgat ctcgaacgga tgatcgccga ggaccgctcg aagaacctgc
     3541 agccctggat ggtcttcgcc agtgcgggca cggtgaacac gggggccatc gatccgctcg
     3601 gggagatcgc caaggtcgcg aaggacgaag ggctatggat gcacgtggac ggcgcctacg
     3661 gggccttctt cgttctcgcg gagcgcgcgc gcgcgtcgct cgaggcgatg gcgctcgccg
     3721 actcggtcgt gctcgatccc cacaagggtc tcttccttcc ctacggctgc ggtgcggtgc
     3781 tcgtgaaaaa cggggaggtg ttgcgccagg cgttcgcggc gacccacaac tatctctccg
     3841 acgtcgagca cgaggagcgg tcgccgtcgt cctactcccc ggagctgacc cgccatttcc
     3901 gcgcgcttcg cctctggatg agcctcaagg tgcacggcat cgagagattc cgctccgccc
     3961 tcgaggagaa gctcctcctg gcccagctcg cctggcagcg gctccgggcc atgccccgga
     4021 tcgaccccgg accggagccg gagctctcct gcgtcgcgtt tcgcgtgcgg gacgaaggag
     4081 acgggccgac gcgcgagatg ctggaccgaa tcttggcgag ggggagagtc cacatgagct
     4141 ccacggtcct gggcgggaag ctctacatta ggatctgcgt gctctgtttc cgatcgcacc
     4201 gggcggatct ggacgaggcg atctcggaaa tcgagcggat cgcgagttaa gcacacccaa
     4261 ctccggaact cctccggttg ggccgggtat gggtcggtat gcagagcttc gcgtggagag
     4321 cggggaccct ctcgctcctg gccgccctcc tcctctcggg gagcgcgctc gcgaagacag
     4381 cccccccttc gccacacgca ccgtccttca cccttcccac ggcgaacggc tccgtctgcc
     4441 tcgactctct ccgcgggcag gcggtcctgg tcgacttctg ggcttcctgg tgcggaccct
     4501 gcaagcagtc gttcccatgg atgaccgccc tgcacgagaa gtacgccacg aaaggactga
     4561 cgatcgtggc gatcaacctc gacaaggatc gcaagctggc cgacgcgttc ctggagcagc
     4621 accccgcccc cttcgtcgtc gccttcgatc catcgggcaa gactgccaag gcgttcaagg
     4681 tatgggggat gcccagcagc ttcctggtcg gagcggacgg cacgattctg ttttcacgag
     4741 cgggtttcga tcccaagaa
//
LOCUS       DASTKC010000174         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_618754, whole genome shotgun sequence.
ACCESSION   DASTKC010000174 DASTKC010000000
VERSION     DASTKC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618754"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFR25_07190"
     CDS             complement(<1..209)
                     /locus_tag="VFR25_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET9940869.1"
                     /translation="MKCPSCGHLEDKVIDSRSAKEGQAIRRRRECLSCHRRFTTYETV
                     ETAPLFVIKKDGRREPFSREKILNGL"
     gene            complement(206..955)
                     /locus_tag="VFR25_07195"
     CDS             complement(206..955)
                     /locus_tag="VFR25_07195"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET9940870.1"
                     /translation="MDLQFLGVPVERHASLGSTNDEALRRAMEGAPEGVVVLADVQTA
                     GRGRQGRAWNDVPGASLAFSVVLRPSLPLPRLPLLALAMACSVAEAAAAVTGRKHDVK
                     WPNDVLFEGRKVCGILAESRSGGGGRPVLVIGTGVNVNHRLEDFPQEFRERATSLRQA
                     AGEYLNPESVLAAVLERFERYVTLSRDRGADALRDAVVDRLPAPGTTVAVRRGDDRVE
                     GVIEGITETGALRFRERGHDEAIVLAAGELL"
     gene            complement(963..1811)
                     /gene="nadC"
                     /locus_tag="VFR25_07200"
     CDS             complement(963..1811)
                     /gene="nadC"
                     /locus_tag="VFR25_07200"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET9940871.1"
                     /translation="MRLEAFALPLVRLALTEDLGGGDVTTDGVVDEAAPGRAHIEARH
                     EGVLAGRELAELTFRELDPEAKVEWTVAEGGTLVKGAKFATIHARARAILSGERVALN
                     FLQHLSGVATLARAYAKAVEGTGVTILDTRKTTPGLRFLEKHAVRVGGAGNHRFGLFD
                     GVLIKENHIRAAGGMKAAFEKTRAGAEGLPTVIEAKTTEEALDAADLHPTRVLLDNFT
                     PSAIAATVKRLKGSKVEVEVSGGIRLANVRDFAIPGVNYISVGSITHSAPALDMSLMM
                     DEVRAG"
     gene            complement(1720..3840)
                     /gene="uvrB"
                     /locus_tag="VFR25_07205"
     CDS             complement(1720..3840)
                     /gene="uvrB"
                     /locus_tag="VFR25_07205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET9940872.1"
                     /translation="MTRDAPAVPFKLHAPYEPQGDQPRAIRELSEGLVRGDKSQVLLG
                     VTGSGKTFTMANVIAAYGKPTLVISHNKTLAAQLYGEFRSFFPENAVGYFVSYYDYYQ
                     PEAYVPQTNTYIEKDASINDDIDRLRLAATSALLERQDVIVVASVSCIYGLGSPEDFR
                     REMLLIQVGEKMTREDILTQLVHDQYARGDFDLKRGTFRARGDVIEIHPAYEETAIRV
                     ELFGDVVDRIAVIDPLTGKTIQPINAAAIYPAKHFVTTPARIEQGLRAIREELTERLG
                     QLENEGKLLEAQRLRMRTEYDMEMLKEVGYCPGIENYSRPLSGRKPGERPGCLIDYFP
                     DDFLMIIDESHVTLPQVGGMYEGDRSRKQVLVEHGFRLPSALDNRPLRFDEFESLLNQ
                     TVYVSATPGELELRKSGGVVVEQIIRPTGLVDPQLAIRPTRGQIDDLLEEIQRRVEVG
                     ERALVTTLTKRMAEDLTEYLFQAGVRVRYIHSDIETIERVEILRALRLKKVDVLVGIN
                     LLREGLDLPEVSLVAILDADKEGFLRSETSLIQTAGRAARHIRGQVILYADHMTGAMR
                     RAIEEMNRRRAVQVAYNEEHGIVPTTIVKSIEDVMRSTSVADAATAIAAEEILPQAAA
                     NLDRNALMDLLEREMLAAAAREDFEVAAVFRDRLDEIRLAPGEPGSPRKEAADAARSV
                     RPAPGPSGTDGGPRGRGRNHRRRR"
     gene            complement(3897..>4859)
                     /locus_tag="VFR25_07210"
     CDS             complement(3897..>4859)
                     /locus_tag="VFR25_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9940873.1"
                     /translation="VLVMNRAEIRQIALREPAEGLITRPSLVWRIRSNGSGTEPLEVS
                     YLANAISWRADYVATLEPNLKSLDLQGWASIENHSGTAFENTKLDLVAGSIHRAGQPP
                     MPMMDRGVAASQAKGGFEQFQERSFSEYHVYELPQRVTLGKDEVKQLGLLEAKSVKST
                     PRYVYDAQRDAQHVVSTIQFANSGGDLGVPIPQGIVRLYQKDKDNTLRLVGEDQVAHT
                     AVRDTVRLTIGTAFDVTAERRQTDQQQVTPRVMESAVEIKLTNQKAESIDVSVVEHSW
                     GDWTITDASHKWRKVDANTFEFVVRCPPGKPVTVTYRLQIQTGG"
BASE COUNT          714 a         1673 c         1625 g          847 t
ORIGIN      
        1 agcccgttca ggatcttctc gcggctgaag ggctcgcgcc tgccgtcctt cttgatcacg
       61 aagagcggcg cggtctcgac ggtctcgtac gtcgtgaagc gccggtggca gctcaggcac
      121 tcgcggcgac ggcggatcgc ctgtccctcc ttggcggagc gggagtcgat gaccttgtcc
      181 tcgagatggc cgcacgacgg gcacttcaca ggagctctcc cgccgcgagg acgatggcct
      241 cgtcgtgccc gcgctcgcgg aagcggagcg cgccggtctc ggtgatcccc tcgatcacgc
      301 cttcgacgcg atcgtcgccg cggcgcacgg ccacggtcgt gccgggcgcg gggaggcgat
      361 ccacgacagc gtctcgaagc gcgtcggcgc cgcggtcgcg cgacagcgtc acgtagcgct
      421 cgaagcgctc gagcaccgcg gcgaggacgg attccggatt caggtactcg ccggcggcct
      481 gccggagcga tgtcgcccgc tcccgaaact cttgaggaaa gtcctccagg cggtgattca
      541 cgttcacacc ggtaccgatc accagcaccg gccggccgcc tccgccgctc cgggactcag
      601 cgaggatgcc gcagaccttg cgcccctcga agagcacgtc attcggccac ttcacgtcgt
      661 gtttccgccc ggtgaccgcc gcggccgctt ccgccacgga gcacgccatc gcgagggcca
      721 ggagcggcag cctcgggagt ggaagcgagg ggcgcaagac caccgagaac gcgagcgacg
      781 cgccggggac gtcgttccac gctcgaccct gacgtccgcg gccagcggtt tgcacgtccg
      841 cgagcacgac gacgccctcg ggagcgcctt ccatcgcgcg ccgaagggcc tcgtcgttgg
      901 tcgagccgag ggacgcgtgc cgctcgacag gcacgcccag gaattgaagg tccatcaccg
      961 catcacccag ctcgcacctc gtccatcatg agcgacatgt ccagcgccgg cgccgagtgc
     1021 gtgatcgatc ccaccgagat gtagttcact cccgggatcg caaagtcccg cacgttcgcc
     1081 agccggatcc ctcccgacac ctcgacctcg accttgctcc ccttcagccg cttcaccgtc
     1141 gccgcgatcg cgctcggcgt gaagttgtcg aggaggacgc gcgtcgggtg gaggtcggcc
     1201 gcatccagcg cttcctcggt cgtcttcgcc tcgatcacgg tcggaagccc ttcggccccg
     1261 gcgcgggtct tctcgaacgc ggccttcatt ccgcctgccg cacggatgtg gttttccttg
     1321 atgagcacgc cgtcgaacag tccgaagcga tggttgcccg caccgcccac gcgcaccgcg
     1381 tgcttctcga gaaacctcaa gccaggcgtc gtcttcctcg tgtcgaggat cgtcacgccg
     1441 gttccctcca ccgccttcgc gtacgcgcgc gccagagtgg cgacgcccga gagatgctgg
     1501 aggaagttca gcgcgacccg ctcgccgctc aggatggcgc gtgcacgggc gtggatggtc
     1561 gcgaacttcg cgcccttgac cagcgtaccg ccctcggcga cggtccactc gaccttcgcc
     1621 tcgggatcca gctcgcggaa cgtgagctcc gcgagctccc tgcccgcgag cacgccttcg
     1681 tgccgggcct cgatgtgggc gcgcccgggc gccgcctcgt caacgacgcc gtcggtggtt
     1741 acgtcccctc ccccgaggtc ctccgtcagt gccagacgga ccaggggcag ggcgaacgct
     1801 tcgagccgca tcagcggcct cctttcgcgg acttccgggc tcgcccggag cgagcctgat
     1861 ctcgtccaac cggtcgcgga acacggcggc tacttcgaag tcctcgcgcg cggcggcggc
     1921 gagcatctct cgctcgagca agtccatgag tgcattgcgg tcgaggttcg cggccgcctg
     1981 cgggaggatc tcctccgcgg cgatcgcggt cgcggcgtcg gccaccgacg tggagcgcat
     2041 cacgtcctcg atcgatttca cgatcgtcgt cggcacgatg ccgtgctcct cgttgtaggc
     2101 gacctgcacc gcgcgccggc ggttcatctc ctcgatcgcg cggcgcatgg cgccggtcat
     2161 gtgatcggcg tagaggatca cctggccgcg gatgtggcgg gcggcgcggc cggcggtctg
     2221 gatcagggac gtctcggacc ggaggaagcc ctccttgtcg gcgtccagga tcgcgaccaa
     2281 ggacacctcg ggcaggtcga gtccctcgcg cagcaggttg atccccacca ggacatcgac
     2341 cttcttgagc ctcagcgcgc gcaggatctc gacccgctcg atcgtttcga tgtcggagtg
     2401 gatgtagcgc acgcgcacgc cggcctggaa gagatactcc gtcagatcct cggccatgcg
     2461 cttggtgagc gtggtgacga gggcgcgctc gcccacttcc acgcgacgct ggatctcttc
     2521 cagaaggtcg tcgatctggc cccgcgtagg gcggatcgcg agctgtgggt cgacgagtcc
     2581 cgtgggacgg atgatctgct ccacgacgac gccaccggat tttcggagct ccagctcacc
     2641 gggcgtcgcc gacacgtaga ccgtctggtt cagcaacgac tcgaactcgt cgaaccggag
     2701 cggccggttg tcgagcgcgg acgggaggcg gaatccgtgc tccacgagca cctgcttccg
     2761 cgaccggtcc ccttcgtaca tgcctcccac ctggggcagg gtcacgtgcg actcgtcgat
     2821 gatcatgagg aagtcgtccg ggaagtagtc gatcaagcat cccggacgct cgccgggctt
     2881 cctccccgag agagggcgcg agtagttctc gatgcccggg cagtatccga cctccttcag
     2941 catctccatg tcgtactcgg tgcgcatgcg gagccgctgc gcttcgagga gctttccctc
     3001 gttctcgagc tgtccgagcc gctccgtcag ctcctcgcga atggcgcgca gcccctgctc
     3061 gatccgggcc ggagtcgtga cgaagtgctt ggccggatag attgcggccg cgttgatggg
     3121 ctggatggtc ttcccggtga gcggatcgat caccgcgatc cggtcgacga catccccgaa
     3181 gagctccacg cggatcgcgg tctcctcgta ggcggggtgg atctcgatca cgtcgccgcg
     3241 ggcccggaag gtgccgcgct tcaggtcgaa gtcgccgcgt gcgtactgat cgtgcacgag
     3301 ctgcgtgagg atgtcctcgc gagtcatctt ctcgccgacc tggatcagca gcatctcgcg
     3361 gcggaagtcc tccggggagc cgaggccgta gatgcaggag acgctcgcga ccacgatcac
     3421 gtcctggcgt tcgaggagcg cgctcgtcgc ggcgagcctg aggcgatcga tgtcgtcgtt
     3481 gatcgaggcg tccttctcga tatacgtgtt cgtctgcggg acgtaggcct cgggctggta
     3541 gtagtcgtag taggagacga agtagccgac cgcattctcc gggaagaagc tccggaactc
     3601 gccgtagagc tgcgccgcca gcgtcttgtt gtgggagatg acgagggtcg gcttcccgta
     3661 cgcggcgatg acgttcgcca tcgtgaacgt cttgcccgag ccggtgaccc cgaggagcac
     3721 ctggctcttg tccccgcgca ccagcccttc cgagagctcg cggatcgcgc gcggctggtc
     3781 gccctgcggc tcgtacgggg cgtgcagctt gaacgggacg gccggcgcgt cgcgcgtcag
     3841 gaggacgcgg cgagccggta tgggtcggga gcgctcgctg gattcctgcg tcgccctcac
     3901 cccccggtct ggatctggag ccggtaggta accgtcaccg gcttcccggg cgggcagcgc
     3961 accacgaatt caaacgtgtt cgcatcgacc ttgcgccatt tgtggctggc atcggtgatc
     4021 gtccagtcgc cccaggagtg ctcgaccacg gacacgtcga tcgattccgc cttctggttc
     4081 gtgagcttga tctcgaccgc gctctccatc acccgcggcg tgacctgctg ctggtcggtc
     4141 tggcgccgct ccgcggtgac gtcgaacgcg gtgccgatcg tgagccgcac ggtgtcgcgc
     4201 accgcggtgt gcgcgacctg gtcctcccca accagccgga gcgtgttgtc cttgtccttc
     4261 tggtagagcc gcacgatgcc ctgcgggatc gggactccga gatccccgcc cgagttggcg
     4321 aactggatcg tggaaacgac gtgctgcgca tcgcgctggg catcgtagac gtaccgcggc
     4381 gtggacttga ccgacttcgc ctcgaggagg ccgagctgct tcacctcgtc cttgccgagc
     4441 gtcacgcgct gcggcagctc gtacacgtgg tactcggaga agctccgctc ctggaactgc
     4501 tcgaacccgc ccttggcctg ggatgcggcg actccgcggt ccatcatggg catcgggggc
     4561 tggcccgcgc ggtggatcga gcccgccacg aggtcgagct tcgtgttctc gaacgcggtt
     4621 ccggagtggt tctcgatgga cgcccacccc tggagatcca ggctcttgag attgggctcg
     4681 agcgtcgcca cgtaatcggc gcgccaggag atcgcgttcg ccaggtacga gacctcgagc
     4741 ggctccgttc cggagccgtt cgaccggatt cgccagacga gcgacgggcg cgtgatcagt
     4801 ccctcggcgg gctcgcgaag cgcgatctgg cgaatctcgg cgcggttcat cacgagaac
//
LOCUS       DASTKC010000175        12977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_856373, whole genome shotgun sequence.
ACCESSION   DASTKC010000175 DASTKC010000000
VERSION     DASTKC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856373"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFR25_07215"
     CDS             <1..865
                     /locus_tag="VFR25_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9940874.1"
                     /translation="GELFEGDRRFPIVVRLPQKSRDDMASLDWLPIPVHGGRHDGGAL
                     AGEGRTEFVPLNAVARVRLEEGPNQVSRENGKRRVVVQANVRGRDLASFVDESRERID
                     ASVRLPAGYWLGWGGQFENLVAARNRLLIVVPLALVLILTMLVLAFGSVKDALVVFSG
                     VPLALTGGVLALLIRGMPLSITAGIGFIALSGVAVLNGVVLLSFVRERLAAGGEVEPA
                     LLEGCARRLRPILMTALVAALGFVPMALATGTGAEVQRPLATVVIGGIVSSTILTLLV
                     LPTLYAVTHRR"
     gene            888..3230
                     /locus_tag="VFR25_07220"
     CDS             888..3230
                     /locus_tag="VFR25_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9940875.1"
                     /translation="MSLAAGTRIGVYEIQGPLGAGGMGEIYRARDTRLDRTVAIKALP
                     QGISSDPALLARFEREAKLLASLSHPNIAGIYGLEDSKGEPYLVLEYVAGETLADRLL
                     RGALSSTETARVGAAIASAIEAAHGRGIVHRDLKPGNVMLTPEGDVKVLDFGIAKGDG
                     VAVGSSAAPSSATVQQTMTGEGVILGTAAYMSPEQARGLAVDRRSDVWSFGCVLFECL
                     AGTRPFQGQTVTDVIVRVLEREPDWSALPGTTPPRLRELLRKCLRKTAGERPDRMADL
                     RSELQSIATEAATAGKSGSSTSPSLAVLYFENLSSDPESEYFCAGITEDILTDLSKVK
                     GMRVASRNAVARFRGTVVDIPKVAAELGVTAVMEGSVRKAGDRVRITAQLINAADGFH
                     LWADRFDRKLEDVFAVQEEIASSITSALRVALTPSEAKEIKRDRPSSARAYDLYLKGR
                     EQYGRYTSESLESALALFRQAIELEPDYALAWAGIGDAYGQLRQWGADDSGGKMLRQG
                     LDAARRAIAIDPKLPDGHKAEALVLRGMGEIEAQTAALKRAHEADPRYTPALCNLAVS
                     LWEGGDIAGAERLYRRALQVDPMEPFAMLWMLQIYEMTGRFEETLTTAAELMRISTIP
                     FYTNGARAMSTLAYLGLGRLADAEAAIDIGSMASGNAELIRALLDYHAGNRNRALELV
                     ERAEAAPQLAPVLAFQGAAILTSLGKRDLARRIMSRPITVPHIQLRIRIMPDLWPIAE
                     EAPYAPARSAMTLIWPSEAPPPKPGVEERFAAVRFESALP"
     gene            3395..3979
                     /locus_tag="VFR25_07225"
     CDS             3395..3979
                     /locus_tag="VFR25_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9940876.1"
                     /translation="MRDAILDAANELLGRHGYNEMTVDDLAAEVGIGKGTIYLHFPSK
                     EEVVLSTIDRMVDRLLVKLAEIAEGPGSVSSRLESMLIARILYRFDHRHHDSKSMDEL
                     LSALRAPYLARRERYFAAEAAVLAEVLEEGRAHHELSVDDVAVTAQTLVLATNALLPS
                     NLSARELGKRKHVAEQATALVRLLVRGLAPPRRS"
     gene            3999..5099
                     /locus_tag="VFR25_07230"
     CDS             3999..5099
                     /locus_tag="VFR25_07230"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9940877.1"
                     /translation="MSRRCTQWLAVFTVVAAVTTPARAQYLIRPSQPDMQVDAATRAV
                     VLDSVVAKLDGQYVIPAVGKKTRDAIAARRKRHEYDRITSAAELGDSLTSHLRAASHD
                     RHLSVRYSYDPVPMSADTLTPAEQAKQDEFFRVRNYGFEQVERLRGNVGYIRLRGFTS
                     SPEAGEAAEAAMRLVSNTDALIIDLRQNGGGNGEMVQLLTTYLLKPSEPVHLVNVYSR
                     PDNITFQSWTLPWVPGPHYLGKEVYLLTSKRTFSAAEEFCFNLQNQKRVTIVGETTGG
                     GAHPVKLERLSEHFQMSVPYARSMNPDTGLDWEGVGVKPDVQVPADDALKTAHMMALK
                     KLKDRPDDPADVAGAIEDLEKTGESRVPTAMR"
     gene            5370..7286
                     /locus_tag="VFR25_07235"
     CDS             5370..7286
                     /locus_tag="VFR25_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HET9940878.1"
                     /translation="MDRDEPRSGARDRVRLERAVDALGLVLVLLLEGFGLVAIAIAVL
                     PCGPAIAATADTTTVAPADTAATSTAASPAKAAPASKPASIKVKVEVLGVTGVLRRNV
                     LASLSIAHKKDRKNSTEGELRHLHSRADEEIGRALQPFGYYRPFIHSELTTGKQWVAR
                     YQIEPGPPLLVDSVLVQVTGEGASDPKFQRLIQDFPVKKGSVLLHQAYEQGKLSLETY
                     AAEGGYLDADFNESRIEVDLARYAATVMVRYDSGPKHFFGPVAFDHAVIDRSMLERFP
                     TFRSGDIFNFRKLQDLQSSLSNTGYFARVEVEPGEETMGHRQVPIEVGLAPAKKLRFT
                     GGVGYGTDDGARVRGLIEMRRINTQGHRAQLNLQYGQRDKRAELQYFIPWPNPRTDVA
                     TLSTGYLDITTQTSTSKIEYGGGSLSRLLGQWRVVPALNYRREKFTVGVDHGTVSTLV
                     PEGTWSRTRTDDQLLTRNGDRVSLNVRGAAEGFVSDVSFAQARVEGKLVHGFPGANRG
                     IARIDVGATNTSDFRELPASFRFFAGGATSVRGYGYNSIGPRDELDHVIGGPYLLVGS
                     LEADHRFLPRWGLAAFFDMGNALNNFGDPLKRGVGTGLRWISPAGLVRLDFAWGLDRA
                     GTPFNIHLTVGPEL"
     gene            7283..11056
                     /locus_tag="VFR25_07240"
     CDS             7283..11056
                     /locus_tag="VFR25_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9940879.1"
                     /translation="MTRHHPRHIKALAIGIALASVIAVGVWWIAYTQEGAKWGFERLG
                     GFLPGNLDVEDMRGPLRGPLEIHHLTYRSERLEITAEHVVLNWRMQDLWRRKLDFTHL
                     HAEGVRVLVGSSGDTQAERDSLYGPLPDLNLPVSIIVRDGLFRNVTVTTPGNDSGLVV
                     DQIAVDARSMRRDSLWVNKFQVRSKMIDLDFRGVALPRGAYPVALNGSWTFRAPDHPA
                     IQGQGTVAGTLDTLRVRQTLSGPLDADVDLRVIKPVRRLGYDGDIEVRRLATRDLDPR
                     LLEATVAGHVHVEGAFDSFSSEGSLHGTTAAAGPARADFRVARARDGVWHIQDLIVTR
                     PGSNARFGAQGTVVMDTTRTAFDLETQWAHVGWPLDKEPLVVSDRGTARIQGTPGDFG
                     IRLNALLAGRNLPPGTWTLNGRGRNGRLAIQTYIADILGGRITGSGEVAWQPRVRWNL
                     AFDATNIDPGTAWPGYPGNLAFTGRTEGTQTPSGPSGTILVSRLGGTLRSQPISGLGT
                     VTASAGQYSAQQAWVQWGPNRVDANGEFGRRWSLDWKLDAPRLAAAMPQASGALRGTG
                     SLQGTPGRMRVIANVTGDSLFVGRMHAGSLRANGDVNLAPGGAIVVNATATEVSEGDH
                     AAGHFELTASGTRDQHQVRVAFAGREDSTVAVLAGGYGGGAWRGQIRSLDTVHPRAGR
                     WSLASPAPLVATREQVAVQNFVWQSGSSRITVNAAWSPSGAWNVDSRFDQVALTLFEP
                     FLPPRIRLTGTMQGEARAGSTTDHHIFADVNVVPGPGEILYMTPTGQWIPNRFDNAAL
                     RASADGGRLHGTLQADLVGNGTVRAEVGSPAYAAAATPVDGHVTIRLRDIGMLQGFTN
                     DLASTAGSLDADLVVGGTMEQPYVQGPVHLRNASADLPRLGLQLREATVEATGSPGGR
                     LAIHGSVLSGGGRLLIDGTAAITRNAEPVAHVTIKGDRVQTLNTKDMQITASPDLIVD
                     VKGQRIDVTGQVTVPQGQMDVGRQDDKAVIKPSSDVVYTDADTLDSAGPWEIHSSVRM
                     ILGDNVTVRGYGLEVKPTGSILTIENPGLPVLARGRLEIHEGTYNIYGQDLEVTEGSL
                     FFAGGPITNPTVRARASRKADDGTVAGFIVRGTVLRPEVSVFSEPAMGQSEALSYILF
                     GKPIEKGNSSQGQVASTMAQTLGVPGTNLLAHNVASELGIEQAKIAVGSSLENTSVML
                     GTHLTSRIFVSAGMDVFEATSTLKVRYVLNRIFTIEAETSRQNRVDLLYTVER"
     gene            11376..12887
                     /locus_tag="VFR25_07245"
     CDS             11376..12887
                     /locus_tag="VFR25_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9940880.1"
                     /translation="MTRASRLDQTPHGGTVRKERMWLLESANASFDPLAAAAALRATG
                     RFAAVVPNFSVHLLATYPSDIWLPYQWYVDDASFADIRLPFAWDIEKGSSSVVIAIID
                     TGVDTSHPDLATQIWHNPGEIAGNGLDDDGNGYVDDADGWDFGRHDNDPRPEYSRDDS
                     TGIDIGFHGTFCAGIAAAATNNDDGIAGAGWNCRIMALKASHPDSTGLIYASAVAPAI
                     AYAVDEGASIISMSFGDFEAPGLVQFYQTLVDMANQAGVLCVAAAGNEGDDVPIYPAG
                     CERVLAVAATDFNNDRAEFSNWGSYVDIAAPGSSMWSTICQNYTFLLSDQIVYLFLGW
                     DTVNPYMYGDGTSFSTPLVAGVAGLVRSRFPNLNPAMVRQHLIATGDVLAFDHPIGPK
                     VNAFQAVNSVPTAVEQGEAPRLMLTAAPNPAAGGSEIRFTLEQDARVRISMFDASGRR
                     VRSLVDGPLAAGPHRIAWDGRTDVGSSLGAGVYLVKLETGVSTRTMKLAWMGR"
BASE COUNT         2132 a         4379 c         4421 g         2045 t
ORIGIN      
        1 gggggagctc ttcgaaggcg accgccggtt tccgatcgtg gtgcgcctgc cccagaagtc
       61 tcgcgacgac atggcgtcgc tggactggct gccgatcccg gtgcacgggg gccggcacga
      121 tggaggcgcc ttggccggag agggacgaac ggagttcgtt ccactgaacg ccgtggcgcg
      181 cgtccgcctg gaggagggtc cgaaccaggt cagccgcgag aacggcaagc ggcgcgtcgt
      241 cgtgcaggcg aacgtgcgcg ggcgcgatct ggcctcgttc gtggacgaat cccgggagag
      301 gatcgacgcc tcggtgcgac tgccggccgg ttactggctc ggctggggcg ggcagttcga
      361 aaacctcgtg gccgcccgga accgcctcct gatcgtcgta ccactcgcgc tagtcctgat
      421 cctcacgatg ctcgtcctgg ccttcggctc cgtcaaagac gcgctcgtcg tcttcagtgg
      481 cgttccgctt gcgctcacgg gaggggttct cgcgctgctc atccgtggga tgccgttgtc
      541 gatcacggcg gggatcggat tcatcgcgct ctccggagtc gcggtgttga acggagtcgt
      601 tctcctgtcc ttcgtgcgcg agcggcttgc cgcgggcggc gaggtggagc cggccctcct
      661 cgaaggctgt gcccgccgct tgagacccat tctcatgacg gccctcgtcg cggcgctcgg
      721 attcgtcccc atggcgctcg cgaccggaac gggagccgag gtgcagcggc cgctcgccac
      781 cgtcgtgatc ggagggatcg tctcctcgac catcctcacg ctgctcgtcc tgccaacact
      841 gtatgcggtg acgcacagga ggtagaacgt ctggtattct cctcgccatg tcgctggccg
      901 ccggaaccag aatcggggtc tacgaaatcc agggacccct cggggccggt ggaatggggg
      961 agatctatcg cgcgcgggac acccgtctcg accgcacggt cgccatcaag gcgctcccgc
     1021 agggcatttc cagcgatccc gcgctcctcg cgcgcttcga gcgcgaggcg aagctcctcg
     1081 cgtcgctcag ccatccgaac atcgcgggca tctacggact ggaggactcc aaaggagagc
     1141 cgtatctcgt gctcgagtac gtcgccgggg agaccctggc ggaccgtctc cttcgaggcg
     1201 cgctctcgtc gaccgagacc gcgcgcgtgg gagccgcgat cgcatccgcg atcgaggcgg
     1261 cgcacgggcg cggcatcgtg catcgcgatc tgaagcccgg gaacgtgatg ctgacgccgg
     1321 agggcgacgt gaaggtcctc gatttcggca tcgcgaaagg ggacggcgtt gctgtcggct
     1381 cctcggcggc gccctcgtcg gcgaccgtcc agcagaccat gaccggcgaa ggggtaatcc
     1441 tcggcacagc ggcctacatg agcccggagc aggcgcgtgg gctcgccgtg gaccggcgga
     1501 gcgacgtgtg gtccttcggg tgcgtgctgt tcgagtgcct ggcgggaacg cggccgtttc
     1561 aggggcagac cgtcaccgac gtgatcgtgc gcgtgctcga gcgcgagccc gactggagcg
     1621 ccctccccgg caccacgcca ccgcggctcc gcgagctgct ccgaaagtgc ctccgcaaga
     1681 cggccggcga gaggcccgac cgcatggcgg acttgaggag cgagcttcaa tccatcgcga
     1741 ccgaggcggc gacggcgggc aagtccgggt cctccacgag cccttcgctt gcggtccttt
     1801 acttcgagaa cctctcgagc gaccccgaga gcgagtactt ctgcgccggc atcaccgagg
     1861 acatcctcac cgacctctcc aaggtcaagg gcatgcgcgt cgcgtcgcga aatgccgtgg
     1921 cgcgcttccg cggcaccgtg gtcgacatcc cgaaggtggc cgcggagctc ggcgtgaccg
     1981 ccgtcatgga ggggagcgtg cgcaaggcgg gggaccgggt gcgcatcacg gcgcagctca
     2041 tcaatgccgc cgacggcttc cacctctggg cggatcgctt cgaccggaag ctcgaagacg
     2101 tcttcgcggt gcaggaggag atcgcgtcgt cgatcacatc ggcccttcgc gtggcgctca
     2161 cgccgagtga ggcgaaggaa atcaagcggg accgtccgtc cagcgcgcgc gcctatgatc
     2221 tctatctcaa gggccgtgag cagtacgggc gttacacctc cgaatcgctc gagagcgcgc
     2281 tcgcgctctt ccgccaggcg atcgagctgg agcccgacta cgcgctcgcc tgggcgggca
     2341 ttggcgacgc ctacgggcag cttcgccagt ggggtgccga cgattcgggc ggcaagatgc
     2401 tccggcaggg actcgatgct gcgcgtcggg cgatcgcgat cgatcccaaa cttcccgacg
     2461 gccacaaggc ggaggctctc gttctgcgcg gcatgggtga gatcgaggcg caaacggccg
     2521 cgctgaagcg cgcccacgag gccgatcccc gctacacccc ggcgctttgc aatctcgcgg
     2581 tctccctgtg ggaggggggc gacattgccg gggcggagcg gttgtaccgg cgggcgctcc
     2641 aggtcgatcc catggaaccc tttgcgatgc tctggatgct tcagatctac gagatgaccg
     2701 gccgcttcga ggaaacgctg accacggcag ccgagctcat gaggatcagc acgattcctt
     2761 tctacaccaa tggcgctcgc gccatgtcca ctctggccta tctagggctc ggccggctcg
     2821 cggacgccga agcggctatc gatatcggct ccatggccag cggaaacgcg gagctcatcc
     2881 gagcgttact cgactatcac gccggaaacc gtaaccgagc gctggagctt gtggagcgcg
     2941 ccgaagcagc gccccagctc gcaccggtcc tggcatttca gggcgcggcc atcctgacct
     3001 cgctgggaaa gagagacctc gcccgccgca tcatgagccg tccgatcacg gtgccccaca
     3061 tccagctcag gatccggatc atgccggatc tctggcccat tgccgaggaa gccccctacg
     3121 ctcccgctcg atccgcgatg accctgatct ggccgagtga agcgccgccg ccgaagcccg
     3181 gggtcgagga gcggttcgcg gccgtccggt tcgagagcgc tttgccctag gtcacgctgg
     3241 gtcgaggtcc cctgcccgaa ggtcccccca gcgaaattct gaaacttccc ggtccccccg
     3301 cccgtcttac tgactgaaac acgattctgg tcaccggtca ttcaggggga ttccgatgca
     3361 ggccacccgc caggttccga gcagccgtca ggcgatgagg gacgcgatcc tcgacgccgc
     3421 gaacgagctc ctggggcgcc acggctacaa cgagatgacg gtcgacgacc tcgcggccga
     3481 ggtcgggatc gggaagggaa cgatctacct ccacttcccg agtaaggagg aggtcgtgct
     3541 ctccaccatc gatcggatgg tggaccggct cctcgtcaag ctggcggaga tcgcggaagg
     3601 acccgggtcg gtctcctcca ggctggaatc gatgctcatc gcgcggatcc tctaccggtt
     3661 cgaccaccgg caccacgact cgaagagcat ggacgagctc ctctccgcgc ttcgagcccc
     3721 ctatctcgcg cggcgcgagc gctacttcgc cgcggaggcc gccgtgctcg ccgaggtgct
     3781 cgaggaaggc cgcgcgcacc acgagctctc ggtcgacgat gtcgcggtca cggcgcagac
     3841 gctcgtcctc gccacgaacg cccttcttcc atcgaatctg agtgcccgcg agctgggaaa
     3901 gcgcaagcac gtcgccgagc aggcgaccgc gctcgtgcga ctcctcgttc gcgggctcgc
     3961 ccctccacgc cgtagctgag gagaccgaaa ccatgcccat gagccgtagg tgtacccagt
     4021 ggctggccgt attcaccgtc gtcgccgccg tcaccacgcc ggcacgcgcc cagtacctca
     4081 ttcgccccag ccagccggac atgcaggtcg acgccgccac gcgcgcggtc gtgctcgaca
     4141 gcgtcgtcgc caagctggac gggcagtacg tgatcccggc cgtggggaag aagacgcggg
     4201 acgcgatcgc cgcgcggcgg aagcggcacg agtacgaccg catcacgagc gcggccgagc
     4261 tcggcgactc gctgacctcg cacctgcgcg cggcgagcca cgaccgccat ctctcggtgc
     4321 gctacagcta cgatccggtt ccgatgtccg ccgacacgct caccccggcc gagcaggcca
     4381 agcaagacga gttctttcgc gtgcggaact acggcttcga gcaggtggag cgcctgcgcg
     4441 ggaacgtcgg ctacatccgc ttacggggat tcacgtcgtc gccggaggcg ggtgaggcgg
     4501 cggaggctgc gatgcgcctg gtctcgaaca cggatgccct gatcatcgac ctgcgccaga
     4561 acggcggcgg caatggcgag atggtgcagc tcctcacgac gtatctactc aagccgagcg
     4621 agccggtcca cctggtcaac gtctactcgc ggccggacaa catcacgttc cagtcctgga
     4681 cgcttccgtg ggtccccgga ccgcactacc tcgggaagga ggtctacctc ctcacgagca
     4741 agcgcacgtt ctcggccgcg gaggagttct gcttcaacct ccagaatcag aagcgcgtga
     4801 cgatcgtggg cgaaaccacg ggcggaggcg cgcatccggt caagttggag cgcttgagcg
     4861 agcacttcca gatgtccgtc ccgtacgcga gatcgatgaa tcccgacacg ggactcgatt
     4921 gggaaggggt cggggtgaag ccggacgtcc aggtgcccgc ggacgacgca ctgaagaccg
     4981 cgcacatgat ggcgctgaag aagctgaagg accgcccgga cgaccccgcg gacgtcgcgg
     5041 gggcgatcga ggatctggag aagaccgggg aatcgagggt cccgaccgcc atgaggtagc
     5101 cccccgccgc gttccggctg ggcgttcgag ccccgcgttc ccccagggac gcggggcttc
     5161 atcctgtaaa aaccgccctt tcttaccact gtattacacg ctatccgccc gcctggccgt
     5221 gcgctccggg ccgggcgaac gaattgcggg cccgcatcga tccccgctcc cgtcggcacg
     5281 ttcgctgctt gagagggtgg acccaagcat ggcgcgggcc atcgcctccc cggactcgcg
     5341 ccccgacttc gtcggacatg gacgggacaa tggatcgaga cgagcccagg agcggagcga
     5401 gagaccgcgt acgcctcgag cgtgctgtcg acgcgctcgg tctcgtgctc gttctgcttc
     5461 tcgaggggtt cgggctcgta gccattgcga tcgcggtcct cccatgcggc ccagcgatcg
     5521 ccgcgacggc ggacacgacc accgttgctc ccgcggacac cgctgcgaca tcgaccgcgg
     5581 cgtccccagc caaagccgcg ccggcgtcga agcccgcctc gatcaaggtg aaggtcgaag
     5641 tgctcggcgt gaccggggtg ctccggagga acgtgctcgc gtcgctcagc atcgcccaca
     5701 agaaggaccg gaagaactcg accgagggcg agctgcgcca tctccattca cgcgcggacg
     5761 aggagatcgg gcgcgcgctc cagccgtttg gatattaccg gccgttcatc cactccgagc
     5821 tgacgacggg gaagcagtgg gtcgcgcgct accagatcga gcccggcccg ccgctcctcg
     5881 tggactcggt gctggtgcag gtgaccgggg aaggcgcctc cgatcccaag tttcagcgcc
     5941 tcattcaaga tttcccggtg aagaagggga gcgtgctcct ccaccaggcg tacgagcagg
     6001 gcaagctgtc gctcgagacc tacgccgccg agggcggcta tctcgacgct gacttcaacg
     6061 agagccggat cgaggtcgat ctcgcgcgct atgccgcgac cgtcatggtg cgctacgaca
     6121 gcgggccgaa gcacttcttc ggtccggtcg ccttcgacca cgcggtgatc gaccggagca
     6181 tgctcgagcg gttcccgacg ttccggtccg gcgacatctt caacttccgg aagctccagg
     6241 acctccagtc gagtctcagc aacaccggtt acttcgcgcg cgtcgaggtc gagcccggcg
     6301 aggagaccat gggccaccgc caggtgccga tcgaggtggg gctcgccccc gccaagaagc
     6361 tccgcttcac cggaggtgtc ggctacggca ccgacgacgg agcgcgcgtg cgcgggctga
     6421 tcgagatgcg ccgcatcaac acgcaggggc accgggccca gctcaacctc cagtacggcc
     6481 agcgggacaa gcgggcggag ctccagtatt tcatcccgtg gcccaatccc cggaccgacg
     6541 ttgccacgct ctccaccggc tacctcgaca tcaccacgca gacgagcacc agcaagatcg
     6601 agtacggcgg cggaagcttg agtcggctcc tcggccagtg gcgcgtggtg ccggccctca
     6661 actaccggcg cgagaagttc accgtgggcg tggatcacgg cacggtgagc accctcgtgc
     6721 cggagggcac gtggagccgc acacgcacgg atgaccagct tttgacccga aacggcgacc
     6781 gcgtgtcgct caacgtgcga ggcgcggcgg aaggattcgt gtcggacgtc tccttcgcgc
     6841 aggcgcgtgt cgaggggaag ctggtccacg gcttccccgg ggcgaaccga gggatcgcga
     6901 ggatcgacgt cggcgccacg aacacgagcg actttcgcga gctcccggcg tccttccggt
     6961 tctttgccgg cggcgcgacg agcgtgcgcg gctatgggta taacagcatc ggacccaggg
     7021 acgagctcga ccacgtgatc ggcgggccgt acctcctcgt cggcagcctc gaggccgacc
     7081 atcgcttcct gccccgctgg gggctcgcgg cgttcttcga catggggaac gccctgaaca
     7141 acttcggcga tccgctgaag cggggtgtcg gcacgggact tcgctggatc tcgccggcgg
     7201 ggctcgtgcg gctcgatttc gcgtggggac tcgaccgtgc gggcacgccc ttcaatatcc
     7261 acctcacggt gggacccgag ctatgacccg ccaccatcca aggcacatca aggcactcgc
     7321 gatcgggatt gcgctcgcgt ccgtgatcgc ggtgggcgtg tggtggatcg cgtacacgca
     7381 ggagggggcg aagtgggggt tcgagcggct cggcggattc cttcctggga atctcgacgt
     7441 cgaggacatg cgaggcccgc tccgtggccc cctcgagatc catcacctga cctaccggtc
     7501 ggaacggctc gagatcacgg ccgagcacgt cgtcctcaac tggcgcatgc aggacctgtg
     7561 gcgccgtaag ctggatttca ctcacctcca tgcggagggc gtccgcgtcc tcgtcgggtc
     7621 atcgggggac acccaggcgg agcgcgactc gctctacgga ccgctgccgg atctcaacct
     7681 cccggtcagc atcatcgtgc gggacgggct cttccggaat gtcaccgtca cgacgccggg
     7741 gaacgactcg ggtctcgtgg tcgatcagat cgctgtcgat gcccgaagca tgcgacggga
     7801 cagcctctgg gtgaacaagt tccaggtgcg atccaagatg atcgacctcg acttccgggg
     7861 cgtggccctg ccgcgcggag cctatcccgt cgcgctcaac gggagctgga cgttccgggc
     7921 tcccgatcat cccgcgatcc aagggcaagg caccgtcgcc ggcacgctcg acacgctgcg
     7981 cgtccggcag accctgtcgg gaccgttaga cgcggatgtc gacctgcgcg tcatcaaacc
     8041 cgttcgaagg ctcggctacg acggcgacat cgaggttcgg cggctcgcga ctcgcgactt
     8101 ggacccgagg ctgctcgagg ccaccgtggc ggggcacgtc cacgtggagg gggctttcga
     8161 ctcgttctcg agcgagggtt cgctccacgg aacgaccgcc gcggccggtc ccgcccgcgc
     8221 cgacttccgt gtcgcgcgcg cccgagacgg ggtgtggcac atccaggatc tcatcgtgac
     8281 gcgcccgggc tcgaacgctc ggttcggcgc gcagggcacc gtcgtgatgg acacgacgcg
     8341 gaccgcgttc gatctcgaga cgcagtgggc gcacgtcggg tggccgctcg acaaggagcc
     8401 cctcgtcgtg agcgatcgtg gaaccgcgcg gatccaggga acgcccggag actttggaat
     8461 tcgtctgaac gcgctcctgg cggggcggaa ccttccgccg gggacgtgga cgctgaacgg
     8521 tcgcggacgg aacggccgcc tcgcgatcca gacctacatc gccgacattc ttggcggacg
     8581 gatcaccggg tcgggcgagg tcgcgtggca gccgcgcgtt cgttggaatc tcgcgttcga
     8641 tgccaccaac atcgaccccg gcaccgcgtg gccgggctat cccgggaacc tcgcattcac
     8701 agggcgtacg gaggggacgc agacgccgtc gggaccctcc ggcacgatcc tcgtgagccg
     8761 tctcggcggc acgctccgga gccagcccat ctcggggctc gggacggtca ccgcgagcgc
     8821 cggacagtac tcggcccagc aggcgtgggt tcagtggggt cccaaccgcg tcgacgcgaa
     8881 cggtgagttc ggccggcgct ggagcctcga ctggaagctc gacgcgcctc gcctcgccgc
     8941 cgcgatgcct caggcgagcg gcgcgctgcg cggtaccggc agcctccagg gaacgcctgg
     9001 gcgcatgcgg gtgatcgcga acgtgacggg ggacagcctc ttcgtggggc ggatgcatgc
     9061 gggatcgctc cgcgcgaacg gcgacgtgaa cctcgctccc ggcggcgcga tcgtcgtgaa
     9121 cgcgacggcc acggaggtga gtgaggggga tcacgcggcg gggcatttcg agctcaccgc
     9181 atccggaacg cgcgaccaac atcaggtccg ggtcgctttc gcggggcgcg aggatagcac
     9241 cgtcgccgtc ctcgccggag ggtacggagg cggcgcctgg cgcggccaga tccgatcgct
     9301 cgatacggtc catccgcgcg ccggccgctg gtcgctggcg agcccggctc cactcgtcgc
     9361 gacgcgagag caggtcgcgg tgcagaactt cgtctggcag tcggggtcgt cgcgcatcac
     9421 ggtcaacgcg gcgtggtctc cttcgggagc ctggaacgtc gattcgcgct tcgatcaggt
     9481 cgcgctcacg ctcttcgagc cgttcctgcc gcctcggatc cgcctcacgg gcacgatgca
     9541 aggggaggcg cgggcgggga gcaccacgga tcatcacatc ttcgccgacg tgaacgtcgt
     9601 ccccgggccg ggtgagatcc tctacatgac gcccacgggg cagtggatcc cgaatcgatt
     9661 cgacaacgcg gcccttcggg cgtcggccga cggtgggcgc ctccacggaa ccttgcaggc
     9721 ggatctggtc ggcaacggga ccgtgcgcgc cgaggtcggc tcgcccgcct acgcggcggc
     9781 cgcgactccg gtcgacggtc acgtgaccat ccgactccgg gatatcggca tgctccaggg
     9841 cttcacgaac gacctggcct cgaccgcggg ctcgctcgac gcggacctcg tcgtgggggg
     9901 cacgatggag cagccctacg tgcagggacc ggtccacctc cggaacgcga gcgcggacct
     9961 gccgcgtctc gggctccagc tccgtgaggc gaccgtggaa gccaccggga gccccggggg
    10021 ccggctcgcg atccatggct cggtcctctc gggtggcgga cggctcctga tcgacggcac
    10081 cgccgcgatc acgcgcaatg ccgagcccgt cgcgcacgtc accatcaaag gggatcgcgt
    10141 ccagacgctg aacaccaagg acatgcagat cacggcctcg cccgatctga tcgtcgacgt
    10201 gaagggacag cgcatcgatg tcacgggaca ggtcaccgtg ccgcaagggc agatggacgt
    10261 cggacgccag gacgacaagg cggtgatcaa gccgtcgagc gacgtggtct acacggatgc
    10321 cgacacgctc gatagcgcgg gaccctggga gatccactcg agcgtccgga tgatcctcgg
    10381 cgacaacgtg accgtgcgcg gctacggcct cgaggtgaag ccgaccggca gcatcctgac
    10441 catcgagaat cccggactcc cggttctcgc gcgcggccgc ctcgagatcc acgaggggac
    10501 gtacaacatc tacggccagg atctcgaggt cacggagggg agcctcttct tcgcgggcgg
    10561 cccgatcacg aaccccacgg tgcgcgctcg cgcctcgcgc aaggccgacg atggaacggt
    10621 ggccgggttc atcgttcgcg ggaccgtgct gcgccccgag gtctcggtct tctccgagcc
    10681 cgcgatgggg cagagcgagg cgctctcgta catcctcttc gggaagccga tcgagaaggg
    10741 gaactcgtcc caggggcagg tggcgagcac gatggcccag accctggggg tgcctgggac
    10801 aaatctccta gcccacaacg tggcgtccga gctgggcatc gagcaggcca agatcgcggt
    10861 cggtagctcc ctcgagaaca cctcggtcat gctcgggacg catttgacgt cgaggatctt
    10921 cgtgagcgcc gggatggacg tgttcgaggc gacctcgacg ctcaaggttc gctacgtact
    10981 gaaccgtatc ttcacgatcg aggccgaaac ctcgcggcag aatcgagtcg atttgttgta
    11041 caccgtcgag cgttaggctg gccctcccac ggggctccgg cgtagactgc gccgaacccc
    11101 aggggaggag gcctgtcgtg aacggacgca cgcccggtac cacccgcgtt tcctcggttc
    11161 tctgccttgc tctccttctc gcggcccact cgagcgccgc gtcccgcatt gctccagcgg
    11221 atcgagcacc cacagcgcga cacatgcctc ccgcgcttcg atacgcgccg ggccagatcg
    11281 tcgcgattgc caaggaaggc gttctggatt cggatgccca gggggcggcg aggtcgccgg
    11341 atggccgaat ccaggcggcg ctttcgaggc tcggcgtgac ccgcgcctcg cgtctcgatc
    11401 agacgcccca cggcggcacg gtgaggaagg agcggatgtg gctcctcgag agcgcgaacg
    11461 cctccttcga cccgctggcc gcagccgcgg cccttcgcgc cacgggacgc tttgcggccg
    11521 tcgtcccgaa cttcagtgtc catctgctcg cgacctatcc cagcgacatc tggctcccgt
    11581 accagtggta cgtggacgac gcaagcttcg ccgacatccg gctcccgttc gcgtgggaca
    11641 tcgagaaggg gagctcgtcg gtcgtgatcg cgatcatcga cacaggcgtc gacacgagcc
    11701 accccgacct cgcgacccag atctggcaca acccggggga gatcgcgggg aacggcctcg
    11761 atgacgatgg aaacggttat gtggatgacg cggacggctg ggacttcggg cggcacgaca
    11821 acgacccgag gcccgagtac tctcgcgacg actccacggg catcgatatc gggttccacg
    11881 ggaccttctg cgccggcatc gcggccgcgg ccacgaacaa cgacgacggc atcgcgggcg
    11941 ccgggtggaa ttgccggatc atggccctga aggcttccca cccggacagc acggggctca
    12001 tttacgcgag cgcggtagcc cccgcgatcg cctacgccgt cgacgaaggt gcgtccatca
    12061 tctcgatgag ctttggagat ttcgaggcgc cgggtctcgt gcagttctat cagacgctcg
    12121 tcgacatggc gaaccaggcc ggcgtgctct gcgtggcggc ggcggggaac gagggcgacg
    12181 acgtgccgat ctatcccgcc ggctgtgagc gcgtgcttgc ggtcgccgcg acggatttca
    12241 acaatgaccg cgcggagttc tcgaactggg ggagctacgt cgacatcgcc gcgccgggca
    12301 gctcgatgtg gtcgaccatc tgccagaact acacgtttct cctgtcggat cagatcgtct
    12361 acctgttcct gggatgggac acggtgaatc cctacatgta cggggacggg acgtcgttct
    12421 cgacgccgct ggtcgcgggc gtcgccgggc tcgttcggtc ccggtttcca aatttgaatc
    12481 cggctatggt gaggcagcac ctgatcgcga ccggggacgt gctggcgttc gaccacccga
    12541 tcggaccgaa ggtgaatgcg ttccaggcgg taaacagcgt gccgacagcg gtcgagcagg
    12601 gcgaggcgcc gcggctgatg ctcacagcag cccccaaccc cgctgcgggt ggatccgaga
    12661 ttcgattcac cctggaacag gatgcgcgtg tgcggatctc gatgttcgac gcttcgggga
    12721 ggcgggtgcg gtcgttggtg gacggtccgc tcgcggcggg accgcaccgc atcgcatggg
    12781 acggtcggac cgatgtgggc tcctccctgg gcgccggcgt ctacctcgtg aagctcgaga
    12841 caggggtctc gacccgcacg atgaagctcg cgtggatggg acggtgagcc tgtcgatcac
    12901 gaccgagcgt cagatcgaga tcgcggcgcc caccgagcgg gtgtggacga tcatctccga
    12961 cgtggagcat tggcacg
//
LOCUS       DASTKC010000176         4868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1093699, whole genome shotgun sequence.
ACCESSION   DASTKC010000176 DASTKC010000000
VERSION     DASTKC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093699"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2204)
                     /locus_tag="VFR25_07250"
     CDS             complement(<1..2204)
                     /locus_tag="VFR25_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940881.1"
                     /translation="MSEHPMTRLDPGAGEMAQRIQAFDWASTPLGPMQSWSPALITTL
                     RIMLANRFPHILWWGPEYIQFYNDAYRPIPGGKHPDRVLGVSCQECWHEIWDVLKPLI
                     DRPFHGGPATWNDDILLEINRHGFLEESHFTIAYSPVPDDTVLSGIGGVLATVHEITD
                     KVVGQRRIGVLRDLGASIHDPKSGEEACAMAAESLREADRDLPFALLYLLDPDARRAQ
                     LAGAAGAVEGEPVSPLAVDLDGPHPWAHAMTTALKRGTITTVRELGSRLTSVPGGPWS
                     DPPDMAAIVPIPASKSGESAGFLVAGVSARLRFDAGYHDFLDLVKTQIGTAIATARAY
                     EHEKKRAELLAELDRAKTTFFSNVSHEFRTPLTLILGPLGDALEAESDPAHRHRLERI
                     QRNALRLQKLVNTLLDFSRIEAGRVQASFEPVDLADLTRDLASSFRSGVEKAGMFLLV
                     NCEPLPEPAYVDRDMWEKIVFNLLSNAFKFTLEGGIEVRLRDAGGRAELTVRDTGAGI
                     APEELPRIFERFHRVEGVEARTHEGTGIGLALASELVKLHGGTLTVESALGRGTTFTA
                     SLPLGRAHLPADRVQAGRSLASTALVAEHYLGEALRWLPGAAEAPAAGPHGDGAPDQP
                     RRPRILWADDNADMRDYVLQLLAPRYDVKCVEDGEAALEAVRREPPDLVLADIMMPRL
                     DGLALLRALRESESTRTLPVILLSARAGEEAALEGLEAGADHYLVKPFSAREL"
     gene            2465..2959
                     /locus_tag="VFR25_07255"
     CDS             2465..2959
                     /locus_tag="VFR25_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940882.1"
                     /translation="MRSATFVRFLFAFTFIAALGGGLGAGCAKRETAEMTQQRTEMTQ
                     DHQAWNSDLQTWETSDSQLRAVMTAPPTGGGAADTTGMSDRLARLEAHEQALTQFRQE
                     VADMDAKLQSYQASDHATLWAENVRLKAEYDRLRSEHDQLVAQLGGAPGMGEARTDTT
                     ASRY"
     gene            complement(2972..3310)
                     /locus_tag="VFR25_07260"
     CDS             complement(2972..3310)
                     /locus_tag="VFR25_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET9940883.1"
                     /translation="MTVLSPEATTPAEIRKKGGERITIRWGDAHESVYPARTLRGRCP
                     CAQCVSETTGKRLVTEEHVRPDVAITAARPVGNYALHFDWSDGHTTGIYSFDYLRRIC
                     PCPACAARRS"
     gene            complement(3312..4340)
                     /locus_tag="VFR25_07265"
     CDS             complement(3312..4340)
                     /locus_tag="VFR25_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET9940884.1"
                     /translation="MDPDLHRDIVSLGFIRNLVIEGDTVSFDVNLTTPACPVKDRLRE
                     QARQAVLQGVPGVRDARVTMTAEVRRPQAPDTSALKTVRNIIAVGSGKGGVGKSTVAA
                     NIAAGLARTGASVGLLDADIYGPSIPIMMKAGRPAEPKQHHIEPGEAFGVKLMSMGYL
                     TPGDQPLIWRGPMAHKAVQQSLLGVNWGELDYLVVDMPPGTGDVHLTLVQTVPVTGAL
                     IVSTPQDVGLQISMKTLRMFQQTKVPVLGIVENMSTYVCPHCGGRDDLFGHGGAREAA
                     NRFGVPFLGEIPLDAAIRRYSDDGRPVVLAEPDSPSGVAFAEITGRLAQQVSIQAFKH
                     IALTIVEE"
     gene            4637..>4868
                     /locus_tag="VFR25_07270"
     CDS             4637..>4868
                     /locus_tag="VFR25_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9940885.1"
                     /translation="MPVSNDELRGLYYYMNLVRQVDERCRRLFKQGRFHGTYFSAVGQ
                     EATVVGSCYGLRAEDVIGIQHREIGAVITKGMP"
BASE COUNT          783 a         1640 c         1670 g          775 t
ORIGIN      
        1 agctcgcggg cgctgaaggg cttgacgaga tagtggtccg cccccgcctc cagtccctcc
       61 agggccgcct cttctccggc gcgcgccgag aggaggatga ccgggagcgt gcgcgtggac
      121 tccgactcac ggagcgcgcg gaggagggcc aggccgtcga gccgcggcat catgatgtcg
      181 gcgaggacga gatcgggcgg ctcccgccgc accgcctcga gcgccgcttc cccgtcctcg
      241 acgcacttca cgtcgtaccg cggcgcgagg agctgcagca cgtagtcccg catgtcggcg
      301 ttgtcgtccg cccaaagaat gcggggccgg cgcggctggt ccggtgcccc atcaccgtgg
      361 ggcccggcgg ctggcgcctc tgcggcaccg ggcagccacc ggagcgcttc gcccaggtag
      421 tgctccgcca cgagcgcggt ggacgcgaga ctccgcccgg cctggacgcg atccgccggc
      481 agatgcgcgc ggccgagcgg gagtgatgcg gtgaaggtgg ttccccggcc caaagcgctc
      541 tccacggtga gcgtcccgcc gtggagcttc acgagctcgc tcgcgagcgc caagccgatg
      601 ccggtgcctt cgtgcgtgcg cgcctcgact ccctccaccc ggtggaagcg ctcgaagatg
      661 cgcggcagct cttcgggagc gattccggcc cccgtgtccc ggaccgtgag ctccgcccga
      721 ccgcccgcgt cgcgcagccg gacctcgatc cctccctcga gcgtgaactt gaatgcgttc
      781 gaaaggaggt tgaagacgat cttctcccac atgtcgcgat cgacataggc gggctcgggc
      841 agcggctcgc aattcacgag taggaacatc cccgccttct cgacgccgga ccggaacgag
      901 ctcgcgagat cgcgcgtcag gtcggcgaga tcgaccggtt cgaacgacgc ctggactcgg
      961 cccgcctcga tgcgcgagaa gtcgagcagc gtattcacca gcttctggag acgaagcgcg
     1021 ttccgctgaa tgcgctcgag ccgatgccgg tgggccgggt cggattccgc ctcgagcgcg
     1081 tctccgagcg gacccaagat caaggtcagc ggcgtccgga attcgtggct cacattggag
     1141 aagaacgtcg tcttcgcacg gtccagctcc gcgaggagct cggcccgctt cttctcgtgc
     1201 tcgtaggcgc gcgccgtcgc gatggccgtg ccgatctgcg tcttcacgag atccaggaag
     1261 tcgtgatagc cggcgtcgaa gcgaagccgt gcgctgaccc ccgccaccag gaagccggcc
     1321 gactccccgg acttcgaggc gggaatcggc acgatggcgg ccatgtccgg cggatccgac
     1381 catggccctc ccggcaccga cgtcaagcgc gagcccaact cgcgcaccgt cgtgatggtt
     1441 ccgcgcttga gcgccgtcgt catggcgtgc gcccacggat gaggtccgtc gagatccacc
     1501 gcgagcgggc tgaccggctc gccctcgacg gctccggcgg cgcccgcgag ctgcgcgcgg
     1561 cgcgcatcgg gatccagcag atagaggagc gcgaacggga ggtcgcggtc cgcctctcga
     1621 agcgactccg cggccatggc gcacgcttcc tcgcccgact tgggatcgtg gatgcttgcg
     1681 cccagatcgc ggaggacgcc gatgcgccgc tgcccgacca ccttgtcggt gatctcgtgc
     1741 acggtcgcga gaaccccgcc gatcccgctc agcaccgtgt catccggcac agggctgtac
     1801 gcgatcgtga agtgggactc ctcgaggaag ccgtgccggt tgatctcgag caggatgtca
     1861 tcgttccacg tcgccggccc tccatggaac ggccggtcga tgagcggctt cagaacatcc
     1921 cagatctcgt gccagcactc ctgacacgac acgccgagca cgcggtcggg atgcttgccc
     1981 cccggaatcg gacgataggc atcgttatag aactggatgt actccggccc ccaccagagg
     2041 atgtggggga atcgattcgc gagcatgatc cggagcgtgg tgatcagcgc gggggaccac
     2101 gactgcatgg ggccaagggg cgtcgacgcc cagtcgaacg cctggatccg ctgcgccatc
     2161 tcgccggcgc ccggatccag cctcgtcatc ggatgctcgc tcattgcggg tagctccctg
     2221 gccgttcgcc ccacctataa aaagcctagc agtgtgagac gcgccgcaat cggaaatctt
     2281 tactcacgag cgttgcaacc gaatccgtga gtgaggatca cgtagtcata cccggccgta
     2341 actccttgcg tgcgagcagc ctcatggcgc ttccacatgg cagacaagtt gcacgggggt
     2401 ccgagcttgc gcgccgacca gctcgggcgg ccgaggccgc taccacgtca aggaggattg
     2461 ggccatgagg tctgccacgt tcgttcggtt cttgttcgcg ttcaccttca tcgccgcctt
     2521 ggggggcggg ctcggtgcgg gctgcgccaa gcgcgagacc gcggagatga cccagcagcg
     2581 caccgagatg acgcaggacc accaggcctg gaactccgat ctgcagacct gggagacctc
     2641 cgactcgcag ctccgcgcgg tcatgacggc gccgcccacg ggcggcggag cggccgatac
     2701 gacgggcatg tccgaccggc tcgcgcgtct cgaagcgcat gagcaggcgc tcacgcagtt
     2761 ccggcaggag gtcgcggaca tggacgcgaa gctccagagc taccaagcgt ccgatcacgc
     2821 gacgctctgg gcggagaacg tgcggctgaa ggcggaatac gaccggctcc gctcggagca
     2881 cgatcagctc gtggcgcagc tcggcggcgc gcccggcatg ggcgaggcgc ggaccgacac
     2941 gacggcctcg aggtactaga gggggatcag ctcaggatcg cctagcggcg cacgccgggc
     3001 acggacagat ccgccgcagg tagtcgaagc tgtagatgcc cgtcgtgtgg ccgtcgctcc
     3061 agtcgaagtg gagtgcatag ttcccgacgg ggcgcgccgc cgtgatcgcg acgtcggggc
     3121 gcacgtgctc ttcggtcacg agccgcttgc cggtcgtctc gctcacgcac tgcgcgcagg
     3181 gacagcggcc gcggagcgtg cgcgcgggat agacgctctc gtgcgcgtcc ccccagcgga
     3241 tcgtgatccg ctcgccccct ttctttcgga tctcggccgg tgtggtcgct tcgggggaga
     3301 gcacggtcac gtcactcctc cacgatcgtg agcgcgatgt gcttgaacgc ctggatgctc
     3361 acctgctgcg cgaggcgtcc cgtgatctcc gcgaacgcga cgcctgacgg cgagtcgggc
     3421 tcggccagga cgaccggccg gccgtcgtcc gagtagcggc ggatcgccgc gtcgagcggg
     3481 atctcgccga ggaacggaac gccgaatcgg ttggccgctt cgcgcgcgcc gccgtggcca
     3541 aacagatcgt cgcggccgcc gcagtgaggg cagacgtagg tgctcatgtt ctccacgatg
     3601 ccgagcaccg gaaccttcgt ctgctggaac atgcggagcg tcttcatcga gatctggagt
     3661 cccacgtcct ggggcgtgga tacgatgagc gcgccggtca cgggcaccgt ctggacgagc
     3721 gtgagatgca cgtcccccgt cccggggggc atgtcgacca cgaggtaatc gagctcgccc
     3781 cagttgacgc caaggagact ctgctggacc gccttgtgcg ccatcggccc gcgccagatg
     3841 agcggctgat cgcccggcgt gagatagccc atcgacatga gcttcacgcc gaacgcctcg
     3901 cccggttcga tgtggtgctg cttcggctcg gcgggacgcc ccgccttcat catgatggga
     3961 atgctggggc cgtagatgtc ggcatcgagg agtccgaccg atgctcccgt cctcgcgagt
     4021 ccggcggcga tattggcggc gacggtcgac ttccccacgc cccccttgcc gctcccgacg
     4081 gcgatgatgt tccggaccgt cttgagcgcc gaggtgtcgg gggcctgcgg cctgcggacc
     4141 tcggcggtca tcgtcacgcg ggcatcgcga acgccgggca cgccctggag caccgcctgc
     4201 ctcgcctgct cgcgcaggcg atccttcacg gggcaggccg gggtggtgag attgacgtcg
     4261 aaggagaccg tgtccccttc gatgacgagg ttccggatga aaccgagact cacgatgtcc
     4321 cggtggagat cggggtccat gacggaccga agcgcatcca agacttgggt ttcgcggatc
     4381 tgaccggcgc tcaaacgctc ctccttcgag ggacatcact cccgtgattc gaatcgcgaa
     4441 tcataacaaa gggacgaggg gcatcgtgtg ccgagttcgc acgaggaacc ctccctatgg
     4501 taggacgccg gcaggggctg gccggtgcgg ccggcggcgg gtggcgcggg gctcgaacat
     4561 ctgatatcgt ggcggttcaa cccgttgccc gtcatcggct ccgcgccttc gatcgagcgt
     4621 cggacatgga ggatgcatgc ccgtttccaa cgatgagctg agaggcctct actactacat
     4681 gaatctcgtg cggcaggtgg acgagcgctg ccgccgcctg ttcaagcagg gacggttcca
     4741 cggcacctac ttcagcgcgg tcggccagga agcgaccgtg gtcgggtcct gttacggcct
     4801 tcgcgccgag gacgtgatcg gcatccagca tcgcgagatc ggcgccgtca tcacgaaggg
     4861 gatgccga
//
LOCUS       DASTKC010000177         6505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_147281, whole genome shotgun sequence.
ACCESSION   DASTKC010000177 DASTKC010000000
VERSION     DASTKC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147281"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..2720
                     /gene="bamA"
                     /locus_tag="VFR25_07275"
     CDS             120..2720
                     /gene="bamA"
                     /locus_tag="VFR25_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET9940886.1"
                     /translation="MIRSRYARRRSQWASAPQAARLSPAALLVAIAAVLAALSLQSGL
                     GVAAPEEPAVGAPASADTTHAAPATHAAPAAPAAPASADTLKSAPVDSSGIGIGAGAG
                     ATAHPDTTAAPLLDIGIPRPEGGSGPVVHSIRASGFANVDSSVIVRTFGLHVGQPYTR
                     DAVREGVRRLFQSGLYTDVNVVDTPAANGVDLNIIVTERARIHAIKFQGLHKIEEKTL
                     KEKVTSAQGQLLDPGTTELDTGKIRDAYAEEGYAHAAVLSRTEVAGPGAVDLVFDVTE
                     GEKVKVRNVVVHQTKPSPGLYANELKDAMKSKPPSFLKSGAYKPSQLETDMTQLRLYM
                     RSRGYKDADVDSIHPEFTSDGKGVDLHVFLHEGDRYRFGNVTWSGNVEVPTAALDFVT
                     VIVRSAPYSEPQIHKTLEGAYTLYQEKGYLYLSIDPKFTERENNIVDVDFQIQEGFRS
                     RVADLQIVGNTRTKENVIRREASVRPGDTFRRSSLVRTQRDIFALGFFQDVTVDYEPT
                     GDSADIKLTLKVQEKQTGTASAGAGFASSTGLTGFIELGHNNLFGNGQSINLHLERGG
                     KRSSFELSFTDPWFRDTPLTLGFSLFNTQREYDVYDRRDVGGGVRFGRPIKWPDYTRG
                     LISYDLRNVSLSNFVAPTVGESSNLTSLRKTDWPRLVSSVTLSFNRISTDNPFYASRG
                     SKMSWIQTFAGGPLGGVEQFYKSVLDVKNYSRLTGPFVLMVRGRTGFLGGESVPEYER
                     FRLGGTTADYVRGYPDYYIVPRDNITKNPVTGTVLERYPGGRRMLIAGAEIQFPIAEP
                     LHGLLFFDAGNTWNSTRDVDLGDLRRSVGIGFRFEVPALGRVGFDLGYGLDREEGAGW
                     RTHFQLGNTL"
     gene            2737..3405
                     /locus_tag="VFR25_07280"
     CDS             2737..3405
                     /locus_tag="VFR25_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET9940887.1"
                     /translation="MTRHRPRCRLLWLGVVLALVLGAPAVRADIKVGFIDSDRIFAEY
                     PKTQEAQTSFNREVQELSRTAKEKKSEIDALQKKLDAQGPMLSEAKRDEQNRDLQRKV
                     ADYETFVQTNWGPGGRISKLNEEFLKPIVDRVHALVTEIGQQEGYSLILDAVDGNIIF
                     GDHTLDLTDRVIQGLNDEDQGKRAPGTLPRATSTASGTTTSGSQPTGNQPNPPVIDNG
                     KDDQ"
     gene            3464..4501
                     /gene="lpxD"
                     /locus_tag="VFR25_07285"
     CDS             3464..4501
                     /gene="lpxD"
                     /locus_tag="VFR25_07285"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET9940888.1"
                     /translation="MKVTLETVAKAIDGTIVGDGSIEITGCAGIREARAGELTFLAHP
                     RYEEYLEHTQASAVIVAENHRHTKKTLVQNSNPYLAYLKAVRFFQGEACPPTPGIHAS
                     AVVAATASVDPTAQIGPHVVLEGGASIGPRTAIQAGCYVGADARVGADCMLYPNVTLR
                     EGCELKDRVIVHSSSVIGSDGFGFIRDGDAYCKLPQVGNVVVEDDVELGACVTIDRAT
                     TGTTRIGAGSKVDNLVQIAHNVQVGRNCIIVAQVGISGSTILRDDVTLAGQVGVIGHI
                     EIGQGAMVGAQSGVAKSVPPGEKMFGAPAQPLALAKRIAASIQNLPDLIQTVRQLKRR
                     VDELERSRAPK"
     gene            4498..5826
                     /locus_tag="VFR25_07290"
     CDS             4498..5826
                     /locus_tag="VFR25_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HET9940889.1"
                     /translation="MSTPSQRTIAKTISYQGTGLHTGGACKVEFRPAPPNSGIRFVRQ
                     DLPGHPEIPVNPRYARADMDQMRRTILKNGQAEVHTVEHILAAAAGLGIDNLVIALDA
                     QEPPEPPDGSATPYVRLFQEAGLVDQPAPRRYFRVTEPVRHEENGVILVALPHDGLRV
                     TFTIEYSNAWRGTQYATYDIDPDVFVKEIAPARTFVLERDVEHLRAKGMIQGGTLQNA
                     VVVKPDGILNREPLRFEDEFVRHKILDFLGDLSLLGRPARGHFLSVKSGHASNVRFVQ
                     RLAATEDSLLGPVSANGSGGPIQLDINAIQRIMPHRYPFLLVDRILSLEPQRVVGIKN
                     VTINEPFFNGHFPGHPIMPAVLIIEAMAQCGGVLLLNSVDRPKERLVYFMGIDNAKFR
                     KPVRPGDQLRFELTLLRLKSRICKMEGKAYVDGDLVAEAELLSSIVDRNP"
     gene            5768..>6505
                     /gene="lpxA"
                     /locus_tag="VFR25_07295"
     CDS             5768..>6505
                     /gene="lpxA"
                     /locus_tag="VFR25_07295"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET9940890.1"
                     /translation="METSSRKQSFYPASSTGIPEVDLLTGTWARAQGTVVPLPGRETA
                     RPLIHPNAIVERGAELGDGVVIGPFAYVGPRVVLGEGTRVGLHAVIEGRTTIGPRCQV
                     HHGAVIGNLPQDLKYRGAPTQVVIGSGTTIREYATVHAATEENEATRIGDNVLLMAYT
                     HVAHNCRLGNNVILANAVNLAGHVRIDDHAIIGGITPVHQFVAVGCHAFIGGGSRVPQ
                     DVPPFVKAAGNPLRAGGLNTIGMERRGI"
BASE COUNT         1189 a         2196 c         2146 g          974 t
ORIGIN      
        1 aagcggagca tccagaagct gatcgaggat cccctctccg aacatgtgct ccgaggccag
       61 ctcaaggatg ggagcgaggt ccgggtggcc cgccagggtg atgagctgac attcacttct
      121 tgatccgatc ccgatacgcg cgccggcgct cccaatgggc gtcggcgccg caggccgccc
      181 ggctcagtcc ggcggccctc ctcgtcgcca tcgccgcggt gctcgcggcc ttgtcgcttc
      241 aatccggtct cggcgtcgcg gctccggagg agcccgcggt gggcgctccc gcgtccgccg
      301 acacgacgca cgcggcgccc gcgacgcacg cggcacccgc ggcacccgcg gcacccgcat
      361 ccgccgatac cttgaagtcc gctcccgtcg actcgagcgg catcggcatc ggcgcgggtg
      421 ccggcgcgac ggcgcacccg gacaccacgg cggccccgct gctcgacatc gggattccgc
      481 gtcccgaagg cggctccggg ccggtcgtgc attcgatccg ggcgtccggc ttcgcgaacg
      541 tggactcgag cgtgatcgtg cggacgttcg ggctccacgt ggggcagccc tacacgcgtg
      601 acgccgtgcg tgaaggcgtg cgccgtctct tccagagcgg cctctacacg gacgtgaacg
      661 tcgtcgacac gccggccgcg aacggggtcg acctcaacat catcgtgacc gagcgggcgc
      721 gcatccacgc gatcaaattc cagggcctcc acaagatcga ggagaagacc ctcaaggaga
      781 aggtcacgag cgcgcagggg cagctcctcg atcccggaac gaccgagctc gacacgggca
      841 agattcgtga cgcctacgcg gaagagggct acgcgcatgc cgccgttctc tcccgcaccg
      901 aagtggccgg gccgggagcg gtggacctcg ttttcgatgt gaccgagggc gagaaggtga
      961 aggtgcggaa cgtggtcgtc caccagacca agcctagccc cggcctctac gcgaacgagc
     1021 tcaaggacgc gatgaagagc aagccgcctt cgttcctcaa gagcggcgcc tacaagcctt
     1081 cgcagctcga gacggacatg acgcagctcc ggctctacat gcggagccgg ggctacaagg
     1141 acgcggacgt cgactcgatc catccggagt tcacgtcgga cgggaagggc gtggacctcc
     1201 acgtcttcct tcacgaaggg gatcgctatc gattcggcaa cgtgacctgg agcggcaacg
     1261 tcgaggtgcc gaccgcggcg ctcgactttg tcaccgtgat cgtccgctcc gccccgtaca
     1321 gcgagccgca gatccacaag acgctcgagg gggcgtatac gctgtaccag gagaagggct
     1381 atctctacct ctccatcgat cccaagttca ccgagcggga gaacaacatc gtcgacgtgg
     1441 atttccagat ccaggagggc ttccgctcgc gcgtcgcgga tcttcagatc gtgggcaaca
     1501 cccggacgaa ggagaacgtg atccggcggg aggcatcggt ccgtcccggg gacacgttcc
     1561 ggcgctcgtc gctcgtccgg acgcagcggg acatcttcgc gctcggcttc ttccaggacg
     1621 tgacggtcga ctacgagccg accggcgaca gcgccgacat caagctgacc ctgaaggtgc
     1681 aggagaagca gaccggaacg gcgagcgcgg gagccggctt cgcgagcagc accggattga
     1741 cgggcttcat cgagctcggc cacaacaacc tgttcggcaa cggccagtcg atcaacctgc
     1801 acctggagcg gggagggaag cgctcgagct tcgagctctc cttcaccgat ccctggttcc
     1861 gcgacacgcc gctcaccctc gggttcagcc tcttcaacac ccagcgggaa tacgacgtct
     1921 acgaccggag ggacgtggga ggaggcgtgc ggttcggccg ccccatcaaa tggcccgact
     1981 acacgcgggg tctgatctcc tacgacctcc ggaacgtgtc cctcagcaat ttcgtcgcgc
     2041 cgacggtcgg ggaatccagc aacctgacct cgctccgcaa gaccgactgg ccacgcctcg
     2101 tctcgagcgt gaccctgagc ttcaaccgga tcagcaccga taaccccttc tacgccagcc
     2161 gtggatccaa aatgagctgg atccagacgt tcgcgggtgg acctctgggc ggggtcgagc
     2221 agttctacaa gagcgtcctc gacgtgaaga actactcccg gttgaccggt cccttcgttc
     2281 tcatggttcg tggacggacc ggattcctgg gaggggagag cgtgccggag tacgagcgat
     2341 tccggctcgg tgggacaacc gcagactacg tgcgcggtta ccccgattac tatatcgtcc
     2401 cgcgggacaa catcaccaag aaccccgtca cggggacagt cttggagcgc tacccgggcg
     2461 gtcggcgcat gctgatcgcc ggggccgaaa tccagtttcc gatcgccgag ccgctccatg
     2521 ggctcctctt cttcgacgcg ggcaacacct ggaattcgac ccgcgacgtc gatctgggag
     2581 acctgaggcg gagcgtgggc atcggattcc ggttcgaagt gccggctctg gggcgggtcg
     2641 ggttcgactt ggggtatggt ctcgaccggg aggagggggc cggctggcgg acccacttcc
     2701 agctcggcaa cacactgtga tacaaggagt tagcttatga ctcgacatcg cccacgctgt
     2761 aggctactct ggctcggggt cgtcctcgcg ctcgtgctcg gggccccggc cgtccgggcg
     2821 gacatcaagg tcgggttcat tgactcggac cgcatttttg ccgagtatcc gaaaacgcag
     2881 gaagctcaga cctccttcaa ccgggaggtg caggagctgt cccgcaccgc caaggagaag
     2941 aagtcggaga tcgacgcgct ccagaagaag ctcgacgcgc aggggccaat gctgagcgag
     3001 gccaagcggg acgagcagaa ccgggatctt cagcggaaag tcgccgacta cgagacgttc
     3061 gtccagacca actggggtcc gggcgggcgg atctcgaagc tcaacgaaga gttcttgaaa
     3121 ccgatcgtcg accgggtgca cgcgctcgtg acggagatcg gtcagcagga gggctactcc
     3181 ctgatcctcg acgctgtcga cggcaacatc atcttcggcg atcacacgct cgacctgacc
     3241 gaccgggtta tccagggatt gaacgacgag gaccaaggga agagggcgcc cgggacgctg
     3301 ccccgcgcga ccagcaccgc cagtggcacc acaaccagcg gcagccagcc tacgggcaat
     3361 cagccaaacc caccggtcat cgacaacggc aaggacgatc agtagcgtcc tttcctagtc
     3421 gggaacgtcg tgggacctga ccgtccacgg cacggagcct aggatgaaag tgaccctgga
     3481 gacggtcgcc aaggcgatcg atggaacgat cgtcggggac ggttcgatcg agatcacggg
     3541 ctgcgccggc atccgcgaag cccgtgcggg ggagctgacg ttcctggccc acccccgcta
     3601 cgaggaatac ctcgagcaca cgcaggcctc cgccgtcatc gtcgccgaga accaccggca
     3661 cacgaagaag accctcgtcc agaattccaa cccctatctt gcgtacctga aggccgtccg
     3721 cttcttccag ggcgaggcct gtccgccgac acccggcatc catgcgagcg cggtggtggc
     3781 ggccacggcc agcgtcgatc cgacggccca gatcggcccg cacgtcgtcc tcgagggggg
     3841 cgcgtcgatc gggccccgca ccgcgatcca ggcgggatgc tacgtgggcg ccgacgccag
     3901 ggtgggagcc gactgcatgc tgtaccccaa cgtgacgctg cgggagggat gcgagctcaa
     3961 ggaccgggtc atcgtccact cgagctcggt gatcgggagc gacggcttcg gattcatccg
     4021 cgatggagac gcctactgca agctcccaca ggtggggaac gtcgtcgtcg aggacgacgt
     4081 ggagctggga gcgtgcgtga cgatcgaccg cgccacgacc ggcaccacgc gcatcggcgc
     4141 gggcagcaag gtcgacaacc tcgtgcagat cgcgcacaac gtgcaggtgg gacggaactg
     4201 catcatcgtg gctcaggtcg gcatctccgg gagcacgatc ctgcgagacg atgtgaccct
     4261 ggcgggccag gtcggcgtga tcgggcacat cgagatcggg cagggggcga tggtcggagc
     4321 ccagtcgggc gtggccaagt ccgtcccgcc gggggagaag atgttcggag cccccgcgca
     4381 gccgctcgcc ctcgcgaagc ggatcgcggc cagcatccag aatcttcccg acctcatcca
     4441 gaccgtgcgg cagctgaagc gccgggtgga cgagctcgag aggtcccgcg ctcccaagtg
     4501 agcactccca gtcagcggac gatcgcgaag accatctcct accaggggac cggcctccac
     4561 accggcggcg cctgcaaggt ggagttccgc ccggcgcctc ccaactcggg cattcgcttc
     4621 gtccggcagg acctgccggg ccatcccgag attccggtca acccgcgcta cgcgcgcgcc
     4681 gacatggacc agatgcggcg cacgatcttg aagaacggcc aggcggaagt gcacacggtg
     4741 gagcacatcc tcgccgccgc cgccggactc ggcatcgaca atctcgtgat cgcgctcgac
     4801 gcgcaggagc cgccggagcc gccggacgga agcgcgacgc cgtacgtgcg cctcttccag
     4861 gaagccggac tcgtggacca gccggcgccg cggcgctact tccgcgtgac cgagccggtt
     4921 cgccacgagg agaacggcgt gatcctggtc gcgctcccgc acgacgggct ccgcgtgacc
     4981 ttcaccatcg aatactcgaa cgcgtggcgc ggcacccagt acgccacgta cgacatcgat
     5041 cccgacgtgt tcgtgaagga gatcgccccc gcccgcacgt tcgtcctcga gcgggacgtg
     5101 gagcacctcc gcgcgaaggg gatgatccag ggaggcacgc ttcagaacgc cgtcgtcgtg
     5161 aagccggacg gcatcctgaa ccgggagccg cttcgcttcg aggacgagtt cgtgcggcac
     5221 aagatcctcg acttcctcgg ggatctctcg ctcctcggcc gcccggcgcg cgggcacttc
     5281 ctgtcggtga agtcggggca cgcgtcgaac gtccgcttcg tccagcgcct cgcggcgacc
     5341 gaggactcgc tcttgggccc ggtgtcggcc aacgggagcg gcggaccgat ccagctcgac
     5401 atcaacgcga tccagcggat catgccgcac cggtatccgt tccttctcgt ggatcggatc
     5461 ctgagcctcg agccgcagcg cgtggtggga atcaagaacg tcacgatcaa cgagccgttc
     5521 ttcaacggac attttcccgg acatccgatc atgcccgccg tcctcatcat cgaggcgatg
     5581 gcccagtgcg gcggggtcct gctcctgaac agcgttgacc ggccgaagga gcggctggtg
     5641 tacttcatgg ggatcgacaa cgctaagttc aggaaaccgg tgcggccagg ggaccagctc
     5701 cgattcgagc tgaccctgct ccgtctcaaa tcccgcatct gcaaaatgga gggaaaggcc
     5761 tacgtcgatg gagacctcgt cgcggaagca gagcttctat ccagcatcgt cgaccggaat
     5821 ccctgaagtc gatctactga ccggaacgtg ggcccgggcc cagggcaccg tcgtgccgct
     5881 gcccggccgc gagaccgcgc gcccattgat ccacccgaac gcgatcgtcg agcggggggc
     5941 ggagctgggg gacggcgtgg tcatcgggcc gttcgcttac gtcggcccgc gcgtcgtgct
     6001 tggcgagggg acccgcgtcg ggctccacgc cgtcatcgag gggcgcacca cgatcggccc
     6061 gcggtgccag gtgcaccacg gcgcggtgat cgggaacctc ccgcaggacc tcaagtaccg
     6121 cggcgcgccg acccaggtcg tgatcgggag cggcacgacg atccgcgagt acgccaccgt
     6181 gcacgcggcc accgaagaga acgaagccac ccggatcggc gacaacgtcc tcctgatggc
     6241 ctacacccac gtggcgcaca actgccgcct ggggaacaac gtgatcctgg ccaacgccgt
     6301 caacctggcc ggccacgtgc gcatcgacga tcacgccatc atcggcggca tcacgccggt
     6361 ccaccagttc gtggcggtcg gctgccatgc cttcatcggc ggcggctcgc gcgtgccgca
     6421 ggacgtgccg ccattcgtga aggcggcggg caatccgctc cgcgccggcg ggctcaacac
     6481 gatcgggatg gagcgacggg gcatc
//
LOCUS       DASTKC010000178         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_856566, whole genome shotgun sequence.
ACCESSION   DASTKC010000178 DASTKC010000000
VERSION     DASTKC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856566"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..982)
                     /locus_tag="VFR25_07300"
     CDS             complement(8..982)
                     /locus_tag="VFR25_07300"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET9940891.1"
                     /translation="MNPNVAAPEAPAEIRRSTARVREILSWYASDNPGTKTNLARMMN
                     HGLLAGTGKMVILPVDQGFEHGPARSFAPNPAGYDPRFHVELAIESGCNAYAAPLGFL
                     EAGASEFAGEIPLILKLNNHDVLHDEKDPISAVTGSVDEALRLGCAAIGFTIYPGSAQ
                     AGHMYEQIRVLAEEAKAVGLAVVVWSYPRGSSITKAGETAIDVVAYAAQIAAQLGAHV
                     IKVKLPTDHIEQDEARKVYEKQAIPIGTLADRVRHVVQSSFDGRRIVIFSGGAKKDND
                     DDVIAEARAIRDGGGFGSIIGRNSFQRKKPEAIRLLRRIMSVYSGETA"
     gene            complement(990..2006)
                     /gene="glk"
                     /locus_tag="VFR25_07305"
     CDS             complement(990..2006)
                     /gene="glk"
                     /locus_tag="VFR25_07305"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET9940892.1"
                     /translation="MILAGDIGGTNARLALFERLDRGRVGPPQALEDLQAHQFPTFEE
                     LLAAYRKRHREPVEIASFGVAGPAVDGKVWGTNLAWDIDTRPVSRLLGAPAHLINDLE
                     ATGYGIPALTDADLELIQEGIPHPRGNAGIVAAGTGLGEAILARVGADLIPVASEAGH
                     ADFGPRTDDEVEFFQLLRARFGRVSWERILSGPGLALIAEWLHARDGAGGPGAAAWGR
                     HVDEAGEHGLPALVSHAGLGGTCPWCVRALEQFVSVYGAEAGNVALRGVTRAGLYLAG
                     GIAPRILPALQKPAFLAAFRDKAPHEKLMAEIPVRVIRNEHAGMIGAARYATLVAAGD
                     GSPL"
     gene            complement(2003..2809)
                     /gene="pgl"
                     /locus_tag="VFR25_07310"
     CDS             complement(2003..2809)
                     /gene="pgl"
                     /locus_tag="VFR25_07310"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9940893.1"
                     /translation="MSGPVPRVRVFPDADSATLGAVEEWSRALARDEEDAVDLAGGAP
                     SPVRRVLFAGGTTPERLYRKLAEPLTMPRAGWRRVEGFFGDERCVPLDHPDSNYGLVR
                     RALLDALGDDAPKVHRMHGEDPDPAEAALAYERAIVDAFRAEADDTPSFDLALLGVGE
                     DGHTASLFPGRSIDPDRLCVATTKPRDGSARLTVTLRLLDAARHVLFFVLGAAKADAV
                     ARCVGPDVTAPREPRPRSLPHDPPPAALVRPPHGRVVWILDQGAARELRA"
     gene            complement(2806..3936)
                     /locus_tag="VFR25_07315"
     CDS             complement(2806..3936)
                     /locus_tag="VFR25_07315"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET9940894.1"
                     /translation="MSEFVRVEGAASRPFDLGELEKDLRAMWRSTSGTTGSPGSEERR
                     RGTVYRAAMSNLVVPLDPERHARMAPILVEITRRHPSRLLLVEIGPGGSRELHAEVAA
                     LCHLRPAGGYICSEQIILRGDAGAGPLVPSAVGALLVGNLPTVLLDLHGGEPRPWIQD
                     LADRADLVLYDTSESPSLDDAGSVWRAIDADTDNCVRDLAWSRLTPWREILAEGFDST
                     HLAPACRNLREVTLEHGTPETDGVPSCLLLLGGWLASRLGWRVRSTEPDGATFDAPQG
                     SVRLRFARDPEGTAGSLHRVQLRSGAPHSLDLRVTHRPAHREATVEVSAPKRQEVEIP
                     FRYRDLAECLMTEIHRHEPNPQLTSAVKCALLLLPTVGDRAA"
     gene            complement(3933..>4521)
                     /locus_tag="VFR25_07320"
     CDS             complement(3933..>4521)
                     /locus_tag="VFR25_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9940895.1"
                     /translation="GAPVPGYCEEPGIHTESQTETYLAVRLDVDNWRWAGVPFYLRSG
                     KRLAKRATEIEIVFRQPPYALFRTAGISELEANVLRMRIQPDEGVSLSFGAKSPGQAL
                     HIDPVRMDFFYLTAFGSDPPDAYDRLLLDCNRGDTTLFARKDEVELAWEIVDSITTAW
                     EKGQGSPLTTYPAGTWGPREADAWIAKDGRKWLHL"
BASE COUNT          666 a         1545 c         1655 g          655 t
ORIGIN      
        1 aggggagcta ggccgtctcg cctgagtaga cggacatgat ccggcgcaaa agccggatgg
       61 cttcaggctt cttcctctgg aagctgttcc ggccgatgat cgagccgaac ccgcccccgt
      121 cgcggatcgc gcgggcttcc gcgatcacgt cgtcgtcgtt gtccttcttg gcgcctcccg
      181 agaagatcac gatccgccgt ccgtcgaagg acgactgcac cacgtggcgc acgcgatcgg
      241 cgagggtgcc gatcgggatc gcctgcttct cgtagacctt ccgggcctcg tcctgctcga
      301 tgtggtcggt cgggagcttc accttgatca cgtgcgcgcc gagctgcgcc gcgatctggg
      361 cggcgtaggc caccacgtcg atcgcggtct cgcccgcctt ggtgatgctc gagcctcggg
      421 ggtaggacca caccacgacc gcgagcccca ccgccttcgc ctcctcggcg agaacccgga
      481 tctgctcgta catgtgcccg gcctgggccg acccgggata gatcgtgaag ccgatcgccg
      541 cgcagcccag gcggagcgcc tcgtccaccg agcccgtgac cgccgagatc ggatccttct
      601 cgtcgtggag cacgtcgtgg ttgttgagct tcaagatgag tgggatctcg cccgcgaact
      661 cggaggcgcc ggcctcgagg aagccgagcg gcgcggcata ggcgttgcac ccggactcga
      721 tcgcgagctc cacgtggaag cgcggatcgt atccggcggg attgggcgcg aaggagcgcg
      781 ccgggccgtg ctcgaagccc tggtcgacgg ggaggatcac catcttgccc gtgcccgcga
      841 ggaggccgtg gttcatcatg cgggccaggt tcgtcttggt tccgggattg tcggacgcgt
      901 accaggagag gatctcccgg acgcgggcgg tggagcggcg gatctcggcg ggcgcttccg
      961 gggcggcgac gtttggattc atgcgcggat tatagggggc tcccgtcccc ggccgcaacc
     1021 agcgtggcgt aacgcgcggc gccgatcatg cctgcgtgct cgttccggat cactcgaacc
     1081 gggatctcgg ccatgagctt ctcgtgcggc gccttgtccc ggaatgcggc caggaaggcg
     1141 ggcttctgga gcgcgggcag gatccgaggc gcgatgcccc cggccaagta gagcccggcg
     1201 cgcgtgactc cgcggagcgc gacgtttcca gcctcggcgc cgtacaccga cacgaactgc
     1261 tcgagcgcac gaacacacca ggggcatgtc ccgccgagtc ccgcgtggct gacgagcgcg
     1321 ggaaggccgt gctcgcccgc ctcgtcgacg tgccggcccc acgcggccgc tcccggcccg
     1381 cctgctccgt cgcgcgcgtg gagccactcg gcgatcagcg cgagaccggg cccggagagg
     1441 atccgctccc agctcacgcg gccgaaccgg gcgcgtagga gctggaagaa ctcgacctcg
     1501 tcgtcggtcc gcggcccgaa atccgcgtgc ccggcttcgg acgcgaccgg gatcaggtcc
     1561 gcgcccacgc gcgcgaggat cgcctccccg agcccggtgc cggcggcgac gatccctgcg
     1621 ttcccgcgcg ggtgggggat gccttcttga atcaactcga gatccgcgtc ggtgagcgcc
     1681 ggaatgccgt agcccgtcgc ttcgaggtcg ttgatcaggt gcgccggtgc gccgaggagc
     1741 cgcgacacgg gacgcgtgtc gatgtcccag gcgagattgg tgccccacac cttgccgtcg
     1801 acggccggtc cggcgacgcc gaacgatgcg atctcgaccg gctctcgatg ccgcttccgg
     1861 taggcggcga gcagctcctc gaacgtcggg aactggtgcg cctggagatc ctccagcgcc
     1921 tgcggcgggc cgacgcgccc acgatccagg cgctcgaaga gcgcgagccg cgcgttcgtg
     1981 ccgccgatgt cgcccgcaag gatcacgcgc ggagctcccg ggcggcgccc tgatcgagga
     2041 tccagacgac gcggccgtgt ggcggacgga ccagcgcggc cggcggtgga tcgtggggaa
     2101 gcgagcgggg gcgaggctcc cgcggcgctg tgacgtcggg tcccacgcag cgcgcgaccg
     2161 cgtccgcctt ggcggcgccg agcacgaaga agagcacgtg ccgcgcggcg tcgagaaggc
     2221 ggagcgtcac ggtgaggcgc gcgcttccat cgcggggctt ggtggtcgcg acgcaaaggc
     2281 ggtcggggtc gatcgatcgg ccggggaaga gggacgccgt gtggccgtcc tcgcccacgc
     2341 cgaggagcgc gaggtcgaac gagggcgtgt cgtccgcctc ggcgcggaac gcgtcgacga
     2401 tggcgcgctc gtacgcgagc gccgcctcgg ccgggtccgg atcctcgccg tgcatgcggt
     2461 ggaccttggg cgcgtcgtcg cccagggcat cgagaagcgc gcggcggacg aggccgtagt
     2521 tggaatcggg atggtccaga ggcacgcagc gctcgtcccc gaagaagcct tccacgcggc
     2581 gccagcccgc gcggggcatc gtcaagggct ccgcgagctt ccggtagagc cgctccggcg
     2641 tggtcccacc ggcgaagagg acgcgcctca cgggactcgg cgcacctccc gccagatcga
     2701 ccgcatcctc ctcgtcgcgc gcgagcgcgc ggctccactc ctccaccgcg ccgagcgtgg
     2761 cggagtcggc atcgggaaac acgcgcacgc gcgggaccgg tccgctcacg cggctcgatc
     2821 tccgacggtg ggaagaagca ggagcgcgca cttgaccgcg ctggtgagct gcggattggg
     2881 ctcgtggcga tggatctcgg tcatgaggca ctcggcgagg tcccggtagc ggaacggaat
     2941 ctccacctcc tgtcgcttgg gcgccgacac ctcgaccgtc gcttcgcgat gcgcggggcg
     3001 atgggtcacc cggagatcga gcgaatgggg ggcccccgat cggagctgga cgcgatggag
     3061 gctgcccgcc gttccctccg gatcgcgcgc gaagcggagc cggaccgagc cctggggcgc
     3121 gtcgaaggtc gcgccatcgg gctctgtgga tcgcacgcgc catccgaggc gcgaagccag
     3181 ccatccgccg aggagcagga ggcaggacgg gacgccgtcg gtttcgggag taccgtgctc
     3241 gagcgtgacc tcgcgcaggt tgcggcacgc gggggcaaga tgggtcgagt cgaagccctc
     3301 cgccaggatc tcgcgccacg gagtcagtct cgaccacgcg aggtcgcgca cgcaattgtc
     3361 cgtgtcggcg tcgatcgcgc gccacaccga gcccgcatcg tccagcgacg ggctctcgct
     3421 cgtgtcgtag aggacgaggt cggcgcgatc ggccagatcc tggatccagg ggcgcggctc
     3481 gccgccgtgc aagtcgagga gcacggtcgg aagattgccg acgaggagcg cccccacggc
     3541 gctcggaaca agcggcccgg ccccggcatc gccgcggagg atgatctgct cgctgcagat
     3601 gtagcccccg gcggggcgaa ggtggcagag cgcggcgacc tcggcatgga gctcccgcga
     3661 cccgcctggg ccgatctcga cgaggaggag ccgcgagggg tggcggcgcg tgatctcgac
     3721 caggatcggg gccatgcgcg cgtggcgctc tggatccaga ggcacgacga ggttcgacat
     3781 ggcggcgcgg tagaccgtac cgcggcgacg ctcttcggat ccaggcgacc ccgtcgtgcc
     3841 cgacgtggat cgccacatgg cgcgaaggtc cttctccagc tcgccgaggt cgaacgggcg
     3901 cgacgcggcg ccctccacgc ggacgaactc gctcataggt ggagccactt ccttccgtcc
     3961 ttcgcgatcc aggcgtccgc ttcgcgcggc ccccaggtgc cggcggggta cgtcgtgagc
     4021 ggagagccct ggcccttctc ccatgccgtc gtgatcgagt ccacgatctc ccaggccagc
     4081 tccacttcgt cctttcgcgc gaagagcgtc gtgtcgccgc ggttgcaatc gaggagcagg
     4141 cggtcgtacg cgtccggcgg atcggagccg aacgcggtga gatagaagaa gtccatacgc
     4201 accgggtcga tgtgcagcgc ctgcccgggg gacttcgcac cgaacgagag cgacacgccc
     4261 tcgtccggtt ggatgcgcat ccgaaggacg ttcgcctcga gctcggagat gccggctgtt
     4321 cggaagagcg cgtacggcgg ctggcggaag acgatctcga tctcggtcgc gcgcttcgcg
     4381 agccgcttgc cggagcggag gtagaaggga acacccgccc agcgccagtt gtccacgtcg
     4441 aggcgcaccg cgagataggt ctccgtctgg gactcggtat ggatgcctgg ctcctcgcag
     4501 tagcccggca ccggcgctcc c
//
LOCUS       DASTKC010000179         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_147600, whole genome shotgun sequence.
ACCESSION   DASTKC010000179 DASTKC010000000
VERSION     DASTKC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147600"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1056
                     /locus_tag="VFR25_07325"
     CDS             352..1056
                     /locus_tag="VFR25_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9940896.1"
                     /translation="MPRAGHVHAIDRIALDVIQCERCPRLREYCLEVARVKRAAYRDE
                     TYWGKPVPGFGDPKAWLLIVGLAPGAHGANRTGRMFTGDKSGEWLYGELHRQGLANRP
                     DSLRANDGLKLDGVYISAAARCAPPDNKPLPVELDRCREYLAREVEALDRVKVVLALG
                     RIGFDAFLKTRRELGRPPVAPKPEFGHGSVSRMPEGVLLLASYHPSQQNTSTGKLTAP
                     MWRSVFTTARTLRDRL"
     gene            1064..1471
                     /locus_tag="VFR25_07330"
     CDS             1064..1471
                     /locus_tag="VFR25_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940897.1"
                     /translation="MIVLFLCRQNAGRSQMAQAFFESMAPPGHVGVSGGSSPAPAIHP
                     VVVDVMREVGIDLAGRVPRKVDAEMLALADRVISMGCDDPAVCDYPGRKVEDWSIEDP
                     SGKPIDRVREIRDELSARVQALVAGLSREEAAR"
     gene            1468..2493
                     /locus_tag="VFR25_07335"
     CDS             1468..2493
                     /locus_tag="VFR25_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9940898.1"
                     /translation="MTAGSMRAVTLVKHGNPSTLRMTECAEPDLLPNEVLIRVRCVGV
                     NFTDVLARQGLNPEAPHPPCILGHETAGDVVAAGSEVTAFRAGQRVLAYHLGGGYAER
                     IAVPATQVFAIPDSIPYQSAVLIPLNYGTAYVALYRTGPVEPGMRVFIHAAAGGVGMA
                     AVDLARRAGLEVVAAASTHFKRARLVAEGVRHVVPAKRMRVHKVSNRLFGGPAFDIVL
                     DSVGGRSIPDGLRSLRPGGRVVSLGVADLSRRGLFGALGFLLGAPRFTYLHLLTASRG
                     LYGVNLRRLMEDAGLVREIIETLVDWAAAGEIRPEPGRVMTLAEAAEAHRILESRGNV
                     GKIVLRT"
     gene            complement(2497..3318)
                     /locus_tag="VFR25_07340"
     CDS             complement(2497..3318)
                     /locus_tag="VFR25_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9940899.1"
                     /translation="MTRSFRSPVLDIWAHLTRPELLARWLGRGDLDLSRGGEFTIEHA
                     NGDTTRGHVIEAVPPVRLELAVRGSTTGRERGLTILLERDGPGSRVTVSETDLREIER
                     TRERRFWTQALAALRSSLQDEAPVSWGADVPVLARAVLPRAAADLWPLIATGPGLDKW
                     VAHVDRFDATAGGAFRLTSRYQGRQVVEEGRIEEITPQSRVVLSWEWAGEGWGAPTRV
                     EFLIEPETSGSSLSVIHSGFENVAADRRLAARKNYAVAWPDVLADLRRLVAPVIH"
     gene            3461..3877
                     /locus_tag="VFR25_07345"
     CDS             3461..3877
                     /locus_tag="VFR25_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940900.1"
                     /translation="MRLWALPTTLVGLVLAGAALFGGRACVRAGTLEAHGGFLGRILR
                     VCPPFAGGAAAMALGHVILATSEEDLARHRDHERVHVRQCERWGPLFLPAYAAASLWA
                     WVRGLDPYMGNAFEREAFAEDEARLAAGSRDGSAAG"
BASE COUNT          586 a         1341 c         1439 g          620 t
ORIGIN      
        1 gccggagcgc cacggggccc cagcggccgc cggtcgaggc ctcgtcggca taggcccacc
       61 cgtacgccgc cacgctcaag gcgtagagga cgggcagggt cgtcgttaag agcggaatca
      121 agggttgcat cagatgggtt caagcctccc cagactgagc cccattgtcc cgccaaacgc
      181 tcgattttac aaggacctgg ggcggagccg gtggggtcat ggcgacctct accttagtac
      241 ccgcgaaggg tgtcgaggtt gccgccgggg accggcgccg gagcggaggc gcggggccgg
      301 gcgctggacc gggcatggtg ggcgctgcgg tgggcgctat agtggctctt gatgccacgt
      361 gccggccatg tccacgccat cgaccggatc gcactcgacg tcatccagtg cgagcgctgt
      421 ccgcgtctcc gggagtactg cctcgaggtc gcgcgcgtga agcgggccgc ctatcgcgac
      481 gagacctact ggggaaagcc cgtaccgggc ttcggcgatc ccaaggcgtg gctcctgatc
      541 gtggggctcg cgcccggcgc gcacggcgcg aaccgcacgg ggcggatgtt cacgggggac
      601 aagtcggggg agtggctcta tggcgagctc catcggcagg gtctcgcgaa tcgccccgat
      661 tcgctgcgcg cgaacgacgg cttgaagctc gacggcgtct acatcagcgc ggccgcgcgc
      721 tgcgccccgc ccgacaacaa gccgcttccc gtcgagctcg atcggtgccg cgagtacctg
      781 gcgcgcgagg tggaagcgct cgaccgcgtg aaggtcgtgc tcgcgctggg acggattggt
      841 ttcgacgcat tcttgaagac gcggcgcgag ctgggccggc ctcccgtcgc accgaagccc
      901 gagttcggcc acggctcggt cagccgcatg cccgagggcg ttctcctcct cgcctcctat
      961 catccgagcc agcagaacac gagcacgggg aagctcacgg cgccgatgtg gcgctccgtg
     1021 ttcacgaccg cccggaccct ccgcgatcgg ctatagtccc atcatgatcg tgctctttct
     1081 ttgccgtcag aacgcgggtc gaagccagat ggcccaggcc ttcttcgaga gcatggctcc
     1141 tcccgggcac gtcggtgtgt cgggcggatc gagccccgcg cccgcgatcc atccggtggt
     1201 ggtggacgtg atgcgcgagg tcggcatcga cctcgcgggg cgagtgccgc gcaaggtcga
     1261 cgccgagatg ctcgccctag ccgaccgcgt gatctcgatg ggctgtgacg atccggccgt
     1321 ctgcgactat ccgggacgga aggtcgagga ctggtccatc gaggatccct cggggaagcc
     1381 gatcgacaga gtgcgggaga tccgggacga gctctccgcg cgcgtgcagg cgctcgtggc
     1441 gggtctttcc agggaggagg cggcgcgatg acggccggat cgatgcgcgc ggtcacgctg
     1501 gtcaagcacg gcaatcccag cacgctgcgc atgacggagt gcgcggaacc cgacctcctg
     1561 ccgaacgagg tgctgatccg ggttcgctgc gtcggcgtga acttcaccga cgtgctcgcg
     1621 cggcagggac tgaatcccga agcgccccat cccccctgca tcctcgggca cgagacggcg
     1681 ggggacgtgg tggccgcggg gagcgaggtc acggcattcc gtgccggaca gcgggtgctt
     1741 gcgtatcatc tgggcggcgg ctatgccgag cggatcgcgg tgcccgcgac ccaggtcttc
     1801 gcgatacccg attcgattcc gtaccagtcg gccgtgctca tcccgctcaa ctacggaacg
     1861 gcgtacgtcg cgctctatcg cacgggcccc gtggagcccg ggatgcgcgt cttcatccac
     1921 gccgccgccg gcggcgtggg gatggcagcc gtggaccttg cgcgccgcgc cggcctcgag
     1981 gtcgtggccg cggcgagcac gcacttcaag cgcgcgcgac tggtggccga gggcgtccgc
     2041 cacgtcgttc cggcgaagcg catgcgggtc cacaaggtct cgaaccgctt gttcggcggt
     2101 cccgcgttcg acatcgtgct cgactcggtc ggcggccggt cgattccgga tggattgcgg
     2161 tcccttcgcc ccggcgggcg cgtcgtgagt ctcggggtgg cggatctctc gcgccgcgga
     2221 ttgttcgggg cgctgggatt cttgctgggc gcgccgcggt tcacgtatct gcacctgctg
     2281 acggcgagcc gcggtctcta cggcgtgaat ctgagacgcc tcatggagga cgcgggcctc
     2341 gtgcgagaga tcatcgagac actggtggac tgggccgcgg ccggcgagat ccggccggag
     2401 ccgggtcggg tgatgacgct cgcggaggct gccgaagcgc atcgaatcct agagtcgcgg
     2461 gggaacgtcg ggaagatcgt gctgcggacg tagccgtcag tggatgaccg gcgcgacgag
     2521 ccggcgcaaa tccgcgagca cgtcgggcca ggccaccgcg tagttcttcc tcgccgcgag
     2581 ccgccggtcg gcggccacgt tctcgaatcc cgaatggatc acgctgagcg acgatcccga
     2641 tgtctccggc tcgatcagga attcgacccg cgtcggcgcg ccccatcctt cacccgccca
     2701 ctcccacgag agcacgacgc gcgactgggg cgtgatctcc tcgatgcgac cctcttccac
     2761 gacctggcgt ccctgatagc gcgacgtgag ccgaaaggct ccgcccgccg tcgcgtcgaa
     2821 acgatccacg tgcgcgaccc acttgtcgag gccgggcccg gtcgcgatca acggccagag
     2881 atccgcggcc gcgcggggaa gaaccgcgcg ggccaaaacc ggaacgtccg cgccccagga
     2941 gacaggcgcc tcgtcctgga gtgaggagcg gagggcggcg agcgcctgag tccagaagcg
     3001 acgttcccgc gttcgctcga tctcgcggag atccgtctcc gagaccgtga cgcgcgagcc
     3061 gggtccgtcg cgctcgagga ggatggtgag accgcgctcc cggcccgtcg tcgatccccg
     3121 caccgcaagc tcgaggcgca ccggaggcac ggcctcgatc acgtgccctc gggtggtatc
     3181 cccattcgcg tgctcgatcg tgaactcccc gccgcgcgag aggtcgagat cgccgcgtcc
     3241 cagccatcga gcgaggagct ccggccgcgt gagatgcgcc cagatatcga gaaccgggct
     3301 tcgaaacgag cgtgtcacgg atgaaggcgg ggcgctcaca tgcgcatcgt acccgatggg
     3361 acgacggctg tcgcggcggg tcgccggcgc gcgccgcgcc cgtccgctcg gtgctaggct
     3421 tccgggcatg gagtccctgg gtggactgag gtcggttgcc gtgcgcctgt gggccctgcc
     3481 gacgacgctg gtagggctgg tcctggccgg cgcggccctg ttcggagggc gtgcgtgcgt
     3541 gcgcgcggga acgctcgagg cgcacggggg attcctgggg aggatcctgc gcgtctgtcc
     3601 ccccttcgcc ggcggcgccg cggccatggc gctgggccac gtgatcctcg ccacatcgga
     3661 ggaggacctc gcacgccacc gggaccacga gcgcgtgcac gtccgccaat gcgagcgatg
     3721 gggtccgctg ttcctgcctg cctatgccgc agcgtcccta tgggcctggg tccgcggcct
     3781 cgacccgtac atggggaacg cgttcgagcg cgaggcattc gcggaggacg aggcaaggct
     3841 ggctgcgggg tcgcgtgacg gctcggccgc aggttagtgt ccgcgcctat cccgctggca
     3901 aagggcgatt cgagacatgc ttcgcgccga gaaactgcaa tgccgcgcgc atacgctctt
     3961 cgatgttgca tacaggtaga cccgcg
//
LOCUS       DASTKC010000180         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_856973, whole genome shotgun sequence.
ACCESSION   DASTKC010000180 DASTKC010000000
VERSION     DASTKC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856973"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..247)
                     /locus_tag="VFR25_07350"
     CDS             complement(41..247)
                     /locus_tag="VFR25_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9940901.1"
                     /translation="MFNLGPQELFWLFLIVLFVFGAKRIPEIGRSIGKGITEFKKGMK
                     DVETEFSTSDRKQVEGGHGEEKKS"
     gene            complement(296..715)
                     /locus_tag="VFR25_07355"
     CDS             complement(296..715)
                     /locus_tag="VFR25_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET9940902.1"
                     /translation="MAKKDSRESRILDFSIGLLLLLIAAFAVSATLRVGPKPAGTPKH
                     PIRIELLNGSGRAGLAGELASYLRDGGFDVLEVSNADRSDYRSTLVVNRTESPQSGRI
                     VAEYLGTRHLIQQVGTQEMIDVTVIVGRDARRWTEPR"
     gene            complement(735..1268)
                     /locus_tag="VFR25_07360"
     CDS             complement(735..1268)
                     /locus_tag="VFR25_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020266856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9940903.1"
                     /translation="MDRLWAGWRMAYIDSARSKKAGKGSCLFCGLAKAKPGPKNLVLA
                     VTPRVLVMMNLFPYNTGHVMVAPTKHVGTLARLDAEDAAEMSDWLGRVETAIGSAYRP
                     DGFNLGMNLGRTAGAGVLGHLHWHIVPRWNGDTNFMPVTASTKVLPEALDRTYAKLAR
                     ALQSAGKPVRRRAGRRS"
     gene            complement(1270..2040)
                     /locus_tag="VFR25_07365"
     CDS             complement(1270..2040)
                     /locus_tag="VFR25_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET9940904.1"
                     /translation="MNPLGLGPFTAVVLAAGLGKRMKSDLPKVLHSALGKPLLLHVLG
                     QLAPLHPSYTVIVVGHKEELVKEALQGRDVHYITQSPQQGTGHAVQVAWPEIEKGAEK
                     GGDTVLILAGDMPLVRTMTIRKLIERHITDRSGVTFLSGELDDPAGYGRVIRDRRGDF
                     QKIVEEKDATAAERKVAEVNSGVYCFRRDILKDALASLRAENEQKEYYLTDTLALVKA
                     RGLRVGVVQASDPRELFGVNTPEQLSMVEQTLRAWGEG"
     gene            complement(2063..>2819)
                     /gene="panC"
                     /locus_tag="VFR25_07370"
     CDS             complement(2063..>2819)
                     /gene="panC"
                     /locus_tag="VFR25_07370"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9940905.1"
                     /translation="HDGHAALFARARGESRRLAVSVFVNPLQFGPREDFARYPRNDLN
                     DARICRGEGVDWLYMPTVDEVYPPGFATQVTPGPMADEYEGAIRPGHFTGVLTVVLKL
                     LETVRPHTLYLGQKDAQQAALVGRMIRDLDLPVHLTVSPTVRERDGVAYSSRNAYLTP
                     EERLRARGLPRALKAARQRAREGATVRSILAEAKRTLQRESKPDRIDYLTVVDPRTFE
                     PIDRLDGRGLLIAAVRIGRTRLIDNMSLSAPRS"
BASE COUNT          419 a          995 c          917 g          488 t
ORIGIN      
        1 ccgcgttgct gctgtcgatc ccaggggcag aacggggact tcagctcttc ttttcttccc
       61 catgcccgcc ctcgacctgc ttccgatccg aggtcgagaa ctcggtctca acgtccttca
      121 tgcccttctt gaactccgtg atccccttgc cgatcgaacg cccgatctcc gggatgcgct
      181 tcgccccgaa gacgaacagc acgatcagga agagccagaa cagctcctgg gggcccaaat
      241 tgaacatcgc gttcgccctt ctctcgagca gcccgtacgg gcggctccgt ggtggtcagc
      301 gcggctcggt ccagcgccgt gcgtcacggc ccacgatgac cgtgacatcg atcatttcct
      361 gggtaccgac ctgttggatc agatgccgcg tgcccaggta ttcggcaacg atccttcccg
      421 actgcggcga ttccgtccgg ttcacgacca gcgtgctccg gtagtcggat cgatccgcgt
      481 tgctcacctc cagcacgtcg aagccgccgt ctctcaggta cgaggccagc tcccctgcca
      541 atccagcccg tccgctcccg ttcaggagct cgatccggat cggatgcttc ggcgtgcccg
      601 ccggcttcgg ccccacccgg agcgtcgccg acaccgcgaa cgcagcgatg aggaggagca
      661 ggagtccgat ggagaagtcg aggatcctcg attcgcgact gtccttcttc gccatcctgc
      721 gacggcctcc gccgtcagct cctccggccc gcgcgtcttc gcacgggttt cccggcgctc
      781 tgaagcgccc gggccagctt cgcgtacgtc cgatccagcg cctcgggcag caccttggtc
      841 gacgcggtca cgggcatgaa gttcgtatcc ccgttccagc gcggcacgat gtgccagtgg
      901 aggtgcccca gcaccccggc gcccgccgtc cgccccaggt tcattcccag gttgaacccg
      961 tccggacggt aggcgctccc gatcgcggtc tcgacccgcc ccagccagtc gctcatctcg
     1021 gccgcgtcct cggcgtcgag ccgggccagc gtgccgacgt gcttcgtggg ggccaccatc
     1081 acgtgccccg tgttgtacgg gaacaggttc atcatcacga gcacgcgcgg cgtgacggcc
     1141 aggaccagat tcttggggcc aggcttcgcc ttcgcgaggc cgcagaagag gcaggatccc
     1201 tttcccgcct tcttggaccg ggcgctgtcg atgtacgcca tccgccagcc ggcccagagc
     1261 cggtccacct caaccctctc cccaggcgcg gagcgtctgc tcgaccatcg agagctgctc
     1321 gggcgtgttc acgccgaaga gctcccgcgg atcggacgcc tggacgacgc cgacccgaag
     1381 accgcgtgct ttgacgaggg cgagggtatc cgtcagataa tactccttct gctcgttctc
     1441 cgctcggagg gacgcgaggg cgtccttcag gatgtcgcgg cggaagcagt agaccccgga
     1501 gttcacctcg gccaccttcc gctccgcggc ggtcgcgtcc ttctcctcca cgatcttctg
     1561 gaagtcgccg cgccggtcgc ggatcacgcg tccatagccc gccggatcgt ccagctcgcc
     1621 cgagaggaag gtcacgcccg agcggtcggt gatgtggcgc tcgatcagct tccggatggt
     1681 catggtccgt acgagcggca tgtcgccggc aaggatgagg accgtgtccc cgcccttctc
     1741 ggcgcccttc tcgatctcgg gccaggccac ctgcaccgcg tgcccggtgc cttgctgcgg
     1801 ggactgggtg atgtagtgaa cgtcgcgccc ctggagcgct tccttcacga gctcctcctt
     1861 gtgccccacg acgatcaccg tgtaggacgg atggagcggc gcgagctggc cgaggacatg
     1921 cagcaggagc ggcttgccca gggcgctgtg gagcaccttg ggaagatcgg acttcatgcg
     1981 cttcccgagc cccgccgcga gtacgaccgc ggtgaagggt ccgagtccga gcgggttcat
     2041 gcgacctccc agacgtggag attcacgagc gcggggcgct cagggacatg ttgtcgatga
     2101 gccgtgtgcg gccgatccgg acagcggcga tcaggaggcc gcggccgtcg aggcggtcga
     2161 tgggctcgaa ggtgcgcgga tcgacgacgg tgaggtagtc gatgcggtcc ggcttggact
     2221 cgcgctggag cgtcctcttc gcctcggcga ggatcgagcg gaccgtggcc ccctcccgcg
     2281 cccgctggcg cgccgccttg agcgcgcgcg gcagcccgcg ggcgcggagg cgctcctcgg
     2341 gcgtgaggta cgcgttgcgc gaggaatacg cgacgccgtc ccgctcccgc accgtcgggg
     2401 acaccgtgag gtgcacgggg agatcgaggt cgcggatcat gcgcccgacg agcgccgcct
     2461 gctgcgcgtc cttctgaccc aggtagagcg tgtgcggccg caccgtctcg aggagcttga
     2521 gaaccaccgt gagcacgccg gtgaaatgcc ccggccggat ggctccctcg tattcatccg
     2581 ccattggccc gggcgtgact tgcgtcgcaa aaccgggcgg atagacctcg tccaccgtcg
     2641 gcatgtagag ccagtcgacg ccttctccgc ggcagatacg cgcgtcgttg agatcgttcc
     2701 tcggatagcg cgcgaaatcc tccctcggcc cgaactggag cggattcacg aagacgctca
     2761 ccgcgagcct ccgactctcg ccgcgcgcgc gtgcgaagag cgcggcgtgg ccatcgtgg
//
LOCUS       DASTKC010000181         9870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1331666, whole genome shotgun sequence.
ACCESSION   DASTKC010000181 DASTKC010000000
VERSION     DASTKC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331666"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFR25_07375"
     CDS             complement(<1..996)
                     /locus_tag="VFR25_07375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9940906.1"
                     /translation="MTPRTTAVVRFSSLGDVLLAAHMPSMLKRANPGERVFFVTKEHY
                     APVLDGHPDIDGLLLLETDDDTGGTPSPASTLLRGALPELAAALREAKVETMFDLHGN
                     LRSAALRHAVRPSRVVRAPKHGLKRRLMVHAKWIPTRPLPPLLRGYRALAGLPADDPL
                     KPWLRDALSARDRARAAEAREGLEPGGYIVYGVGARWATKRWPLEHFVSLAAAVRKEW
                     GLEARFALSPGEPDHELVRLLGADAHQAVTLDVRALAALAEDAAAIVSNDSGVLHLGP
                     ALGIPVVGLFGSTVPGFGFALQGPRDAVAEIPLPCRPCSVHGLGRCPLGHHACMKA"
     gene            complement(1004..2974)
                     /locus_tag="VFR25_07380"
     CDS             complement(1004..2974)
                     /locus_tag="VFR25_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9940907.1"
                     /translation="MRRGRPKQRSASLRLLQYLRPYRGHLLLTAALMVGFALTSGISI
                     GMISPFVKILFTPRHAAQAPATLPAPIAAVTGLDTTAPHPVPDAAQSEEAKGGIPARV
                     SIWKKDLRTWFEHFFLTGNPITSLTRICLTLLIVFFLKNVFDYLQSVLTVYVEQAVVR
                     DLRNEVYGHLHELSLSFFHTRRTGALLSRLTNDIALVRGALAAGFSNFVKSILLLTVC
                     LFWIFWTSWRLALVSCLVVPPSVFLIVWLGKKLRRRSTITQERMADLNSILQETLTGI
                     RVVKAFSMEEFEKQKFARVAQAYFRSFVKQRRLGALAGPLSETLGVFAATLVLWYGGH
                     EILLQRSMEPQQFFIFLFAMLQLMSPLKSLSNVNATIQEGLAAAVRIFRLLDTKPTVV
                     SRPGARVALGIQEGIDFDRVSFRYGGGPEVLSDVTFQVRAGEVVALVGPSGAGKSTLA
                     DLVARFYDPTEGSIRLDGKDLRDYDVASHRRVMGVVTQESILFNDTVWNNIAYGVTDA
                     DEEAVRRAARAANAEQFILDMPEGYQTTIGDRGVLLSGGERQRIAIARAIFKNPSLLL
                     LDEATSSLDSQSELLVQEALDALFAGRTAFVIAHRLSTIQRADRIFVLDKGRIVQTGT
                     HRELVATPGLYQKLHRLQFRLADQLVAQNRAG"
     gene            complement(2980..3381)
                     /locus_tag="VFR25_07385"
     CDS             complement(2980..3381)
                     /locus_tag="VFR25_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HET9940908.1"
                     /translation="MMTDQDSAILDLALKHGQYHPNAYRFTLDAVNHAVRCAREIRHV
                     SGAEVLEGARELALARFGPVAKTVLEQWGIGTTEDFGAIVFQMVEQGLLGKTPEDKPS
                     DFARGYDFEETFVRKFDWLDRIGGRRPSELE"
     gene            complement(3383..5038)
                     /gene="nadB"
                     /locus_tag="VFR25_07390"
     CDS             complement(3383..5038)
                     /gene="nadB"
                     /locus_tag="VFR25_07390"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET9940909.1"
                     /translation="MTPTTRKRSARSQTETDVLVLGSGIAGLTVALHAAQFARVIVAT
                     KVDALESNTNYAQGGVAAALGDDDAFARHERDTLVCGAGLCDPEAVHVLVEEGPTEVM
                     RLISLGVRFDKDAADPDRLALGREGGHSRRRIVHAKDRTGHAIERVLLAQARAHPKVT
                     ILENAIAIDLVLESKHLAGRRLAPSRERVWGAYLLDRGTGSIDVVAAHATVIATGGCG
                     KVYTYTTNPDIATGDGLAAGFRAGAPVANLEFMQFHPTCLYHPEAKSFLISEAVRGEG
                     AKLLTTDGKPFMQRYHRLRDLAPRDIVARAIDFEMKRRGDKFVLLDMARLGRKLLERR
                     FPHITRRVQELGYDVFKEPIPVVPAAHYMCGGIVTDLDGRVELNGLYACGEVACTGVH
                     GANRLASNSLLEAMVFSRRAGRAILRQVSADPRAGLPRLPAWRTNGAVEPKEQVIFDH
                     NWDALRQVMWDYMGIVRSDERLARAAAMVAVLREQTESDYWRYRLDADLIELRNIGLV
                     ADLIVACARRRSESRGLHYNVDHPRTNDRQFRRDTVLTRQDVR"
     gene            complement(5035..6204)
                     /gene="lpxK"
                     /locus_tag="VFR25_07395"
     CDS             complement(5035..6204)
                     /gene="lpxK"
                     /locus_tag="VFR25_07395"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET9940910.1"
                     /translation="MSGPPTSDRGFAGSLERSWSGRPGTMAWTLALAPLSLGYAAGSA
                     VARARASSSRRGIDGAPVLCVGGLTVGGSGKSSLVRWLAEAILARGGRPAVVSRGHGR
                     EDATGTLVLPDTTDYPAERLARFGGDEAAALRAALDASTVVAVGRDRRRSAVRAIAGY
                     GATSVLLDDGWEQGSLAWDRLWVAVDPIHPFGNGWMLPAGPLRRPPRTLREAHVVAFV
                     LEPGDEIGDATLRAVREHAPRAAVLRFRRSLDRRTPLARHDDLADASLSGKTVALVTA
                     VGAPERVERLVAASGARVVHHASFPDHARFREDDLARALERGASAGASEALITEKDEA
                     RWPHSLRASLPVHVLRTRLTPLDPVEPHLVGLVPAGGAVSRDALGFTSSGATRSS"
     gene            complement(6201..7544)
                     /locus_tag="VFR25_07400"
     CDS             complement(6201..7544)
                     /locus_tag="VFR25_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HET9940911.1"
                     /translation="MSAGLAIYRALTQSVGAFLGPLPPAPDASPWRAGRTGAGEETAR
                     AAGSVWIHAASLGEVGAARAWARALAARRYRPPFLLTTRTAAGLARARADLEGLVAAR
                     IAPHDLPQIVDAVLDDACPWRLDIIETEIWPNLIVESRKHGVAVALVGASVSERTTSR
                     LMGLGVAGASLLGAGVYALPQSDAHARRFARLGVPEARIRVIGDLKAEPPLDQGSRPF
                     GSRPALVFGSLRPGEEHVARLVADTLERHRARALAARASWPDERRDGDPFAGRSRAVL
                     IVAPRHAAGEVLAKAALAQAGYTVDVRDERTRSDRTLEAWIEELASRKGPRAGLLATR
                     GELPSAYERAWGAIVGGTFSEHGGHNVWEAAARGCPVLVGPHHDEVAVAVDAVRAEGG
                     AIAAADERHAIVAVEGWLLDEDLVQRGLASIRAAARAAGAADRGLDAIDSWGLAP"
     gene            complement(7552..8826)
                     /gene="lpxB"
                     /locus_tag="VFR25_07405"
     CDS             complement(7552..8826)
                     /gene="lpxB"
                     /locus_tag="VFR25_07405"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET9940912.1"
                     /translation="MGGVRDGARGAPRRRADRAPDRPAHAAGRRSLTFPKIIVSAGDP
                     SGDRLGAACLTALRRRGVPVEAAGLGGPALRAAGLHAVAPMESVAVMGFGGVVRHLPA
                     IRRARARVLELLEDTPDAIFLPIDSPGLNLGLARAAKRMGRPVVYYVLPQIWAWGYGR
                     IRRIREDVDLMLLLFRFEEAICRSEGVAARWVGHPAGALAPDPARREEARRAFEVDSG
                     DTLIALLPGSRKGEVRHHLAPMLDAALRLVSHAPGRVRVAVSDAGPAEEERTRGATRG
                     LWDSLRPIQVRGESEPLLRAADLALVASGTATLEAASIGVPLVIVYRTSALNFAIARR
                     LVRLPRIGLANIVMGQDVAPERIQDAATGASIAAAGARLLEDPAERERQRSIFAGLPE
                     RLGGAGAAERAADAILEFVQSGRAAQAPAARG"
     gene            complement(8730..9710)
                     /locus_tag="VFR25_07410"
     CDS             complement(8730..9710)
                     /locus_tag="VFR25_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9940913.1"
                     /translation="MTKARVGVSGLGHLGSHHARAWKKIDGITLVGGHDPDPAARARA
                     EKDLGITTFESLSALLDQVDLISLASPTPAHADGAIEALGRGIHVLVEKPMASRPEEG
                     ERMLSAARTAGVVLYVGHVERHNPAVAASLPHLRSPKFIEAHRLAPPVNRGLDVDVIL
                     DLMIHDLDLALLATRSRITEIHAVGVPVLTSKIDIANARLVFESGCVANLTASRISRD
                     KVRKIRFFEGSEYLSIDCMNRSVEAYALTPGGPLGGGATTAELLARIRPVPIEVKDLD
                     PLETELRHVLALRDEPHRSWAESATALEALRAAERIGRLTAPLTPQGVAA"
BASE COUNT         1482 a         3498 c         3539 g         1351 t
ORIGIN      
        1 ggccttcatg caggcgtgat ggccgagcgg acagcggccc aggccatgga cgctgcaggg
       61 acggcacgga agcgggattt ccgcgaccgc gtcgcgcgga ccctggaggg cgaacccaaa
      121 ccccgggacg gtgctcccga agagtccgac cactgggatg ccgagcgccg gtcccaagtg
      181 gaggacgccg gagtcgttcg acacgattgc cgccgcgtcc tcggccaggg ccgcgagcgc
      241 gcgcacgtcg agcgtgaccg cctgatgggc gtcggctccc aggagccgca cgagctcgtg
      301 atccggctcg cccggcgata gcgcgaaccg tgcctcgagc ccccactcct tccggaccgc
      361 ggccgcgagc gacacgaagt gctcgagcgg ccagcgcttc gtcgcccagc gcgcaccgac
      421 tccgtacacg atgtaacccc ccggctcgag accctcgcgc gcctcggcgg cgcgggcgcg
      481 gtcgcgcgcc gagagcgcgt cccgaagcca cggcttgagc ggatcgtcgg ccggaagtcc
      541 cgcgagcgcg cggtaaccgc gaaggagcgg cggaagcggg cgggtcggga tccacttcgc
      601 gtggaccatg agccgtcgct tcaatccatg cttgggagct cgcacgaccc gcgacggccg
      661 aaccgcgtgc cggagcgccg ccgatcggag gttgccgtgg agatcgaaca tcgtctcgac
      721 cttcgcctcg cggagcgccg cggcgagctc gggaagggcg ccacggagga gcgtcgacgc
      781 cggcgacggc gtgcctcccg tgtcgtcgtc ggtctcgagg agcaaaaggc cgtcgatgtc
      841 gggatgaccg tcgagcacgg gggcatagtg ctctttcgtg acgaagaaaa cgcgctcgcc
      901 tgggttcgcg cgcttcagca tcgagggcat gtgcgcggcg aggagcacgt cgcccagcga
      961 ggagaaccgg acgaccgccg tggtgcgcgg cgtcatgcgc ggatcagccc gctcgattct
     1021 gcgcgaccag ctggtcggcg aggcggaact ggaggcggtg gagcttctgg tagagccccg
     1081 gcgtcgcgac cagctcccgg tgcgtcccgg tctggacgat tcgccccttg tcgagcacga
     1141 agatgcgatc ggcgcgctgg atggtggaga gccggtgcgc gatcacgaag gccgtccggc
     1201 ccgcgaagag cgcgtcgagc gcttcctgca cgaggagctc cgactgcgag tcgaggctcg
     1261 acgtggcttc gtccaggagg aggagcgacg ggttcttgaa gatcgcccgc gcgatcgcga
     1321 tgcgctggcg ctcgcctccc gagaggagca cgccgcgatc tccgatcgtc gtctggtagc
     1381 cctccggcat atcgaggatg aactgctccg cgttggccgc gcgcgcggcc cggcggactg
     1441 cttcctcgtc cgcgtcggtg acgccgtagg cgatgttgtt ccagacggtg tcgttgaaca
     1501 ggatcgactc ctgcgtcacg acccccatca cgcgtcggtg cgacgcgacg tcgtagtcgc
     1561 ggagatcctt cccgtcgagc cggatcgatc cctcggtcgg gtcgtagaag cgcgcgacca
     1621 gatcggccag cgtcgacttc cccgctccgc tcgggccgac cagcgccacg acctcgcccg
     1681 cgcgcacctg gaaggtcacg tcggagagga cctccgggcc gccgccgtac cggaaggaga
     1741 cgcgatcgaa gtcgatcccc tcctggatgc ccagcgccac gcgcgctccc gggcggctca
     1801 cgaccgtcgg cttggtgtcg agcagccgga agatccgaac cgcggccgcg agcccttcct
     1861 ggatcgtcgc gttgacgttg ctgagcgact tgagcgggct catgagctgg agcatcgcga
     1921 agagaaagat gaagaactgc tgcggctcca tggagcgctg gagcaggatt tcatggccgc
     1981 cgtaccagag gacgagcgtg gcggcgaaca ccccgagcgt ctcggagagc ggtcccgcca
     2041 gggcgccgag tcggcgctgc ttcacgaacg agcggaagta ggcctgcgcc acgcgcgcga
     2101 acttctgctt ctcgaactct tccatggaga acgccttcac gacgcggatg ccggtcagcg
     2161 tctcctggag gatggagttg aggtcggcca tgcgctcctg ggtgatcgtg ctccgccgcc
     2221 gtagcttctt ccccagccag acgatgagga acaccgaagg cggcacgacg aggcaggaca
     2281 cgagcgcgag ccgccacgag gtccagaaga tccagaagag gcagaccgtc aggagcagga
     2341 tgctcttcac gaagttgctg aacccggccg cgagcgcccc tcgcacgagt gcgatgtcgt
     2401 tcgtgaggcg cgagagcagc gctccggtcc ttcgcgtgtg gaagaacgag agcgagagct
     2461 cgtgcaggtg gccgtagacc tcgttccgga gatcccgcac cacggcctgc tccacgtaca
     2521 cggtgagcac gctctgcaag taatcgaaca cgttcttcag gaagaagacg atgaggagcg
     2581 tgagacagat ccgcgtgagc gaagtgatgg ggttccccgt caggaagaag tgctcgaacc
     2641 aggtgcggag gtccttcttc cagatgctca cgcgcgccgg gatcccgccc ttcgcctcct
     2701 cggactgcgc cgcgtcgggc accgggtgcg gcgcggtcgt gtcgagtccc gtcacggcgg
     2761 caatcggagc cggaagcgtc gcgggagcct gggccgcatg gcgcggcgtg aagaggatct
     2821 tcacgaatgg agagatcatc ccgatcgaga tgccgctcgt gagggcgaag cccaccatga
     2881 gcgccgccgt gaggaggaga tgcccccgat agggcctcag gtactgcagg aggcggagcg
     2941 aagcgctccg ctgcttggga cggccgcggc gcaccctgac tactcgagct ccgagggtct
     3001 gcggccgccg atccggtcca gccagtcgaa tttccgcacg aaggtctcct cgaagtcgta
     3061 gccgcgggcg aaatcgctcg gcttgtcctc gggcgtcttc ccgaggagcc cctgctccac
     3121 catctggaag acgatggctc cgaaatcctc cgtcgtcccg atgccccact gctcgagcac
     3181 ggtcttggcg acggggccga accgcgcgag cgcgagctcc cgggcccctt cgaggacctc
     3241 ggccccgctc acgtggcgga tctcgcgcgc gcaccgcacc gcgtggttca cggcgtcgag
     3301 cgtgaaccgg taggcattgg ggtggtattg cccgtgcttc agggcgagat cgagaatggc
     3361 gctgtcttga tcggtcatca tgtcagcgga cgtcctggcg ggtgagcacc gtgtcgcgac
     3421 gaaactggcg gtcgttcgtg cgcggatggt cgacgttgta gtggagtccg cgcgactcgg
     3481 agcgacgccg cgcgcaggcc acgatcaaat cggccacgag cccgatgttc cgaagctcga
     3541 tcaggtccgc atcgagccgg tatcgccagt aatcactttc ggtttgctcc cggagcacgg
     3601 ccaccatggc ggccgcccgg gcgaggcgct cgtcgcttcg gacgatgccc atataatccc
     3661 acatgacctg tcggagcgca tcccaattgt ggtcgaagat gacctgctcc ttgggctcca
     3721 ccgcgccgtt cgtgcgccat gccggaagcc gcggcagccc cgcgcgcgga tcggccgaca
     3781 cctggcgcag gatcgcccgc ccggctcgcc gcgagaagac catggcctcg aggagcgagt
     3841 tcgacgcgag tcgatttgcc ccatgtacgc cggtgcaggc gacctcgccg cacgcgtaga
     3901 gcccgttcag ctccacccga ccgtccagat cggtcacgat gccgccgcac atgtagtggg
     3961 ccgcgggcac gaccgggatc ggctccttga agacgtcgta gcccagctcc tgcacccgcc
     4021 gcgtgatgtg cggaaagcgg cgctcgagaa gctttcgccc gagccgggcc atgtcgagca
     4081 gcacgaactt gtccccgcgg cgcttcatct cgaagtcgat cgcgcgcgcc acgatgtcgc
     4141 gcggcgcgag atcgcgcagg cggtggtagc gctgcatgaa cggcttccca tccgtggtga
     4201 gcagcttcgc cccttccccg cgcaccgctt cggagatcag gaacgacttc gcctcgggat
     4261 gatagaggca cgtggggtgg aactgcatga actcgaggtt cgcgaccggc gctccggcgc
     4321 ggaaccccgc cgcgagtccg tcccccgtcg cgatgtcggg attcgtcgtg tacgtgtaga
     4381 ccttgccgca gccccccgtc gcgatcacgg tcgcgtgcgc cgcgacgaca tcgatcgacc
     4441 ccgtgccgcg gtcgagcagg tacgcgcccc agacacgctc gcgcgacggc gcgagccttc
     4501 gcccggcgag gtgcttcgac tccaagacca gatcgatcgc gatcgcgttc tcgaggatgg
     4561 tgaccttcgg gtgtgcgcgg gcctgggcga ggagcacgcg ctcgatcgca tgacccgtcc
     4621 ggtccttggc atggacgatc cggcggcgcg agtgtccccc ctcgcggccg agcgcgagcc
     4681 ggtcgggatc cgccgcgtcc ttgtcgaagc ggacgccgag cgagatcaac cgcatgacct
     4741 cggtgggccc ctcctccacg agcacgtgga ccgcctcggg atcgcagagc cccgccccgc
     4801 agacgagcgt gtcccgctcg tgccgggcga acgcatcgtc gtcccccagg gcggccgcga
     4861 cacccccctg ggcatagttc gtgttcgact cgagcgcgtc caccttggtc gcgacgatga
     4921 cccgcgcgaa ctgcgccgcg tggagcgcga cggtcaggcc ggcgatgccg cttcccagga
     4981 ccaggacatc ggtctcggtc tggctgcgcg cgcttcgttt gcgagtggtg ggtgtcatga
     5041 ggatcgggtg gcccccgatg aggtgaagcc tagggcatcc ctgctgacgg cgccaccggc
     5101 cgggacgagg ccgacgagat gcggctcgac gggatcgagc ggcgtgagcc gggtccggag
     5161 cacgtgcacg gggagggacg cacgtaaaga atgcggccat cgagcctcgt ccttctcggt
     5221 gatgagtgct tcgctggcgc cggccgatgc gccgcgctcg agcgcgcgcg cgaggtcgtc
     5281 ctcccggaag cgcgcgtggt cggggaacga cgcatgatga acgacccggg ctccggacgc
     5341 ggcgacgagt cgctccacgc gctccggcgc tcccacggcg gtcacgagcg cgacggtttt
     5401 gccagacaga gacgcatcgg ccaggtcgtc atggcgggcc agcggggtcc ttcgatcgag
     5461 cgagcgtcgg aagcggagca cggcggcgcg aggcgcgtgc tcgcgaaccg cccggagcgt
     5521 cgcgtcgccg atctcgtcgc cgggctcgag cacgaacgcc acgacgtgcg cctcccgaag
     5581 cgtcctcggc ggccgccgca acggaccggc gggtagcatc cagccgtttc cgaatggatg
     5641 gatgggatcg accgcgaccc agagccggtc ccacgcgagc gatccctgct cccatccgtc
     5701 gtcgagcagc accgaggtcg cgccgtatcc cgcgatcgcg cgcaccgccg agcggcgccg
     5761 gtcgcgtccc accgcgacca ccgtgctcgc gtcgagcgcg gcccgaagcg cggcggcctc
     5821 gtcgccgccg aaccgcgcga ggcgctcggc gggatagtcc gtcgtatcgg gcagcaccaa
     5881 ggtcccagtc gcatcctccc ggccgtgtcc gcgcgagacc acggcggggc gcccgccgcg
     5941 ggccaggatc gcctccgcga gccaacgcac gaggctcgac ttccccgatc cccccacggt
     6001 gagtcccccc acgcagagga cgggcgctcc gtcgatcccc cggcgcgacg acgacgcgcg
     6061 cgcgcgcgcg accgccgagc ctgcggcgta gcccagggag agcggtgcga gcgcgagggt
     6121 ccaggccatc gtcccgggac gccccgacca ggatcgctcg agcgatcccg cgaagccgcg
     6181 gtccgacgtc ggggggccgc tcacggcgcg agcccccagg aatcgatcgc gtcgagcccg
     6241 cggtcggccg ccccggccgc gcgcgcggcg gcgcggatgg aggcgaggcc gcgctgcacg
     6301 agatcttcgt cgaggagcca gccttcgacc gcgacgatcg cgtgccgctc gtcggcggcc
     6361 gcgatcgcgc ccccctcggc ccgcacggcg tccaccgcca cggccacttc gtcgtggtgg
     6421 gggcccacga gcacgggaca tccgcgcgcg gccgcctccc agacgttgtg ccctccgtgc
     6481 tccgagaacg tgccgccgac gatcgccccc cacgcgcgct cgtaggcgga ggggagctct
     6541 ccgcgcgtcg cgaggagtcc cgcgcgggga cccttccggc tcgcgagctc ctcgatccat
     6601 gcctcgagcg tccggtccga gcgcgtgcgc tcgtcgcgca catcgaccgt gtagcccgcc
     6661 tgggcgagcg ccgccttcgc cagcacctct cccgccgcgt gcctgggagc gacgatcagc
     6721 accgcgcgcg agcgcccggc aaacggatcg ccgtcgcggc gctcgtccgg ccacgaggcg
     6781 cgcgcggcca aggcccgcgc ccgatgccgc tcgagcgtgt ccgcgacgag tcgcgccaca
     6841 tgctcctcgc ccggcctcaa gctcccgaag acgagtgccg gcctcgagcc gaacggccgc
     6901 gatccctggt cgagcggcgg ttccgctttg aggtcgccga tgacccggat ccgcgcttcg
     6961 ggaacgccga gccgcgcgaa gcgccgcgcg tgcgcatcgc tctgcggaag cgcatacacg
     7021 cccgcgccga gaagcgacgc ccccgccacg ccgagcccca tgagccgcga tgtggtgcgc
     7081 tccgacacgg aggcgcccac gagcgccacc gcgacgccgt gctttcgcga ctcgacgatc
     7141 aggttgggcc agatctcggt ctcgatgatg tcgaggcgcc acggacatgc gtcgtccagc
     7201 acggcgtcca cgatctgcgg gagatcgtgc ggcgcgatgc gcgccgccac gagcccctcg
     7261 agatccgcgc gcgcccgcgc gagtccggcg gcggtacgcg tggtgaggag gaacgggggc
     7321 cggtaccggc gcgccgcgag cgccctggcc cacgcgcgcg ccgcgcccac ctccccgagc
     7381 gacgcggcgt ggatccagac cgatcccgcc gcgcgcgcgg tttcctcgcc cgctccggtt
     7441 cgccccgcgc gccagggact cgcgtcgggc gccggaggaa gaggtcccag gaatgcgccc
     7501 acgctctgcg tgagcgcgcg gtagatcgcg aggcccgcgc tcatggcggg ctcagccgcg
     7561 cgcggccggg gcctgggccg cgcggccgct ctggacgaat tcgaggatgg cgtccgcggc
     7621 gcgctccgcg gcgcccgcgc cgccgagccg ctccggaagg ccggcgaaga tcgatcgctg
     7681 gcgctcccgc tccgccggat cctcgaggag ccgcgctccg gcggccgcga tcgaggcacc
     7741 ggtggccgcg tcctggatgc gctccggggc cacgtcctgc cccatcacga tgttcgcgag
     7801 cccgatgcgc ggcaatcgca cgagccgccg cgcgatcgcg aaattcaaag cgctcgtgcg
     7861 atagacgatc acgagcggca ccccgatcga ggccgcctcg agcgtcgcgg ttccggacgc
     7921 gacgagcgcg agatcggcgg cgcgaaggag cggctccgac tcgccgcgga cctggatcgg
     7981 ccggagcgag tcccagaggc cgcgcgtcgc gccccgcgtg cgctcctcct cggcgggtcc
     8041 ggcgtcgctc acggcgacgc gcaccctgcc cggcgcgtgg ctcacgaggc ggagcgcggc
     8101 gtcgagcatc ggggcgagat ggtggcgcac ttctcccttt cggctgcccg ggaggagcgc
     8161 gatcagcgtg tccccggaat ccacctcgaa agcgcggcgc gcttcctcgc gcctggccgg
     8221 atcgggcgcg agcgcgcccg cggggtgccc cacccagcgc gcagcgaccc cctcgcttcg
     8281 gcagatcgcc tcctcgaatc ggaagaggag gagcatgaga tccacgtcct cccggatgcg
     8341 gcggatgcgg ccgtagcccc acgcccagat ctggggcagc acgtagtaca cgaccggacg
     8401 ccccatgcgc ttggctgcgc gggcgaggcc gaggttgagc cccggcgagt cgatcgggag
     8461 aaagatcgcg tcgggcgtgt cctcgaggag ctcgagaacc cgcgcgcgcg cgcgccggat
     8521 ggcgggcaga tgtcgcacga cgccgccgaa ccccatgacc gcgaccgact ccatcggcgc
     8581 gacggcgtga aggcccgcgg cgcgaagcgc ggggccgccc aggcccgcgg cctccaccgg
     8641 gacgccgcga cgccggagcg cggtcaagca cgccgcgccc agccggtccc ccgacgggtc
     8701 gccggcggag acgatgatct tcgggaaggt caggctgcga cgccctgcgg cgtgagcggg
     8761 gcggtcaggc gcccgatccg ctcggcggcg cggagcgcct cgagcgccgt cgcggactcc
     8821 gcccacgatc ggtgcggctc gtcgcgcaag gcgaggacgt ggcggagctc ggtctcgagc
     8881 gggtccaggt ccttgacctc gatcggcacg ggacggatcc gggcgaggag ctcggcggtg
     8941 gtggcgccgc ccccgagagg gccgcccggc gtgagcgcat acgcctcgac cgagcggttc
     9001 atgcagtcga tcgagaggta ctcgctccct tcgaagaacc ggatcttccg gaccttgtcg
     9061 cgcgagatgc ggctcgcggt gagattcgcg acgcacccgc tctcgaacac gaggcgggcg
     9121 ttcgcgatgt cgatcttgga ggtgaggacc ggcacgccga cggcgtggat ctcggtgatg
     9181 cgcgagcgcg tggcgaggag cgccaagtcg agatcgtgga tcatgagatc gaggatcacg
     9241 tcgacgtcga gaccgcggtt cacgggaggc gcgagccgat gtgcctcgat gaacttgggc
     9301 gagcggagat gcggcaggga tgccgccacg gcgggattgt gccgctcgac gtgcccgacg
     9361 tagagcacga cgccggccgt gcgcgccgcg ctcagcatcc gctctccctc ctccgggcgg
     9421 gaagccatcg gcttctcgac cagcacgtgg atgccgcggc cgagcgcctc gatcgcgccg
     9481 tcggcgtggg cgggggtcgg tgacgcgagc gagatgagat cgacctggtc gaggagcgcc
     9541 gagagcgact cgaaggtggt gatgccgaga tccttctccg cccgcgcgcg cgcggcggga
     9601 tcgggatcgt gcccgcccac gagcgtgatg ccgtcgatct tcttccaggc gcgggcatgg
     9661 tggcttccga ggtgccccag ccccgaaacg ccgacccgcg ccttggtcac cgcacgatgc
     9721 cccgctccga gctccggacg aactcgacga actcgtggac ctcgggatag ggtttgagct
     9781 cgcgcaggat gcggtcgagc gcctgcgcca cgttgagctg cgagcggtag aggatccggt
     9841 acgcgcgctt gagctcgagc cgcgcctcgg
//
LOCUS       DASTKC010000182         8395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1804548, whole genome shotgun sequence.
ACCESSION   DASTKC010000182 DASTKC010000000
VERSION     DASTKC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804548"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..2094
                     /locus_tag="VFR25_07415"
     CDS             376..2094
                     /locus_tag="VFR25_07415"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9940914.1"
                     /translation="MRYTQAFLPTLKEDPADAELVSHKLMVRGGMLRKLAAGIYTILP
                     LGWRVTSKVARIVREEMDRAGCQEVLLPILMPAELWKESGRWDKYGPELFRQRDRHQR
                     DFALGPTHEEAITDLVRNHVRSYRDLPLNLYQIQTKFRDEIRPRFGVMRAREFMMKDG
                     YSFHVDEASLGQGYERMHEAYSAVFRRCGLEFVVVEADSGAIGGDVNHEFMVTADSGE
                     SMVFSSACGYAASEESAKYAVPEHAAEAAQALAPKDTPGKKSVEEVAEFLKVTPDRLL
                     KSLVFIAGDDPVLAVVPGDRELNEAKLARALGGVPIRLATAQEIEKITGGPLGFTGPV
                     GLESKVHTLYDASLGNSRNYVTGGNKLDVHLTNVRFGRDLTGGTQADLSTAREGDRCP
                     RCGDVMRVTRGIEVGHIFKLGLRYSEPMKATFLDPEGVERTIVMGTYGIGVTRTVAAV
                     IEQLHDDNGMIWPISIAPYQVHIVAVNVKDDTTRGAADQLYAALQERGIEVLYDDRDE
                     RPGAKFKDADLLGIPLRVTVGEKGLKEGIVELRDRRTGVVEKLPVQDAADAVARRVRD
                     DLTKLS"
     gene            complement(2078..2344)
                     /gene="rpsT"
                     /locus_tag="VFR25_07420"
     CDS             complement(2078..2344)
                     /gene="rpsT"
                     /locus_tag="VFR25_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET9940915.1"
                     /translation="MPHHKSAVKRMRTAKRSATKNASVKSEIKTYLKKMQETPGDKEL
                     ARMTASRLDRAVRKGVIPKAVANRRKSRLAKMANRAAAKTTKAS"
     gene            2448..4343
                     /gene="uvrC"
                     /locus_tag="VFR25_07425"
     CDS             2448..4343
                     /gene="uvrC"
                     /locus_tag="VFR25_07425"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9940916.1"
                     /translation="MAPRLPKRRGDALPPDPPDDVAKADLKQAIAALPAQPGVYLFRD
                     RNGQLLYVGKAKRLDARVRSHFQDPEALGPRQVVLTRRTQAVEYIAVDSEIDALLLEA
                     TLVRERQPPFNVRLKDDKRYPYLRVSWQEPFPRLTVVRKVEKDGARYFGPYTEVKSLR
                     ALLRLTQTIFPMRTCTDIDAHIQAKRECLEFHIGRCTGPCIAAQSAEDYRAMVDQFCD
                     FLAGKREEVVARLRALQENAAERREYERAARLRDQIKKLEQILARQRMVDIAGLSADV
                     LGLAREGERCCVVVLKVRERRVVAKDVRWLRGTSGKTEGELLRAFVTLYYSQTEAIPE
                     ALVVETDPAECELLVGYLERGAGGPVRVRQPRDGTERALLRTAHRNATMLVSRAREAA
                     GKGLEGNAADEALELQRALGIDRLPRRIRCFDVSQLFGTYVVASMVTFIDGRPFKNEY
                     RHYRIRTVEGQDDFASIAEAVRRHAGRVTEGDMEAADLVLIDGGIGQLQAALAAVQGT
                     PLESVPFAGLAKRLEEIVRPGGAPVLRLSRRSPALKLLMRVRDEAHRFAIVYHRTLRG
                     RAVRSTELEKVPGLGPKRQALLLRRFGSVERLKQQPVDAIAAVPGIGERMAARILEVV
                     GSAGGPQ"
     gene            4340..4972
                     /locus_tag="VFR25_07430"
     CDS             4340..4972
                     /locus_tag="VFR25_07430"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9940917.1"
                     /translation="MSSSTQHARSASPRSATKRTSTRLARRKAEREPVAAASVKTPLG
                     KIWILATESGLREIRLADQGRPTRPEMDARGWKLVRKKKWTDPVRRILERYLQGREQR
                     LEIELDLAIGTPFQRRVWDAARRIPYGAVVSYAELASMAGGSGAQRAVGNALGANPVP
                     IVVPCHRIIHADQSIGGFSSGLRWKRHLLTIERGQLELTWTKPRRSLFGR"
     gene            4969..5892
                     /gene="xerD"
                     /locus_tag="VFR25_07435"
     CDS             4969..5892
                     /gene="xerD"
                     /locus_tag="VFR25_07435"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET9940918.1"
                     /translation="MTRPAKSASQPATTPLGADLERFREHLALERRMSEHTVRAYVRD
                     LEQYGRFLGGRKIRTAGSASARDIEEYMREAKWAASTVARKLAALRAFHEFLRRRGVA
                     GDNPALQVRPPRRSRPLPDVLSVPQVEALLKAPRGEEPAQIRDRAMLEVAYATGLRVT
                     ELVKLRIEEIDGEEQLVRCMGKRSRERIVPYGNQAKRALEEYLDRARPGLVRDRSERA
                     VFLTRLGRSFSRMGFWKLLKGYAKAAGIEQPVSPHTLRHSCATHLLEGGCDLRVVQEI
                     LGHRSIETTQIYTHLDRGYLREVHTRFHPRA"
     gene            5921..6673
                     /locus_tag="VFR25_07440"
     CDS             5921..6673
                     /locus_tag="VFR25_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940919.1"
                     /translation="MTRIGDRPGRGGWALGLAMFAVLSIMALGNARAQESDPNGLDPD
                     AQIALGPIRYASSVSLGMRQPPPTQERPRAPLLRRQRPNTISIGIQGDYGIVRGNSRL
                     ADGFDDGPGYSFRFRYMLAPSFALGFSFESQRFYDKGGIPSTEIGAGDSTVVMTTVGA
                     EGVFYIHREREVNPYFVTGFGYASPDVVDEVRGSARVNEGMFLVLGSGLERFIRPRFS
                     LDFTLRGYGLISNSEFTGIATLSAGIHLYPGD"
     gene            6680..8068
                     /locus_tag="VFR25_07445"
     CDS             6680..8068
                     /locus_tag="VFR25_07445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9940920.1"
                     /translation="MRVTFWGAARTVTGSKHLLTGESGTVLLDCGLFQGRRAESEARN
                     RALPFNGAAIDAVVLSHAHIDHSGALPVLCRSGFDGKIHATRTTVDLCRPMLLDAAHL
                     QMKDAEFMNKRRNHNHKGDPVVPLYTAGDVECVLERMVGEEYETPFEPIPGVSVMFHE
                     AGHIAGAASVSLRWKENGREGSLVFSGDLGRPGRPILKDPVPLQKADYLITEGTYGGK
                     HHPDEETLWTSFEAVLKDAIARGGRIVIPAFAVGRTQEVVYALDHLFRSGRVPEMPIY
                     IDTPLGIEAQTIIRSHPEVFDEETAAALRAGGDPFGMQRATLIKDAEDSKKLNDKPGT
                     FITVSASGMCEGGRILHHLKQTVGDAKHTIVIIGYQAEGTLGRRLVEHVPEVKIFGEP
                     YEVHAKVVVLNGFSAHADHDALVAQAAACGAPKGVAIVHADLERAEALKAGLPSPERV
                     RIPTEGHTWELA"
     gene            8065..>8395
                     /locus_tag="VFR25_07450"
     CDS             8065..>8395
                     /locus_tag="VFR25_07450"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940921.1"
                     /translation="MTREERRGRYHGFRVGDRVLHKEFGEGLIVEVRDRDGFEVLEIA
                     FPDGIRRLTSLYPLEPARGAGRATSAPAVSAATSAPALRLGTDLPARGQFRIAPGADE
                     LVQRLVAG"
BASE COUNT         1380 a         2793 c         2918 g         1304 t
ORIGIN      
        1 caagcttatc gattgggagt cgattggtga acgtagctcg atctacgttt ggtcctgggg
       61 tcgcatgccc cgcgaaagcc gatacttcag aatcgacttc gaccgcaagg cggcgaccca
      121 tcctactgtc actcgcatag acggaccacc ggatcatgta acaagacgca cgaacaccgt
      181 tccgctcgag tttaggcagg aaggtggcac tatgtctgta cgaaccaggg aatccttgga
      241 atggagtaga tttcgcgccg cccgtggtag ggtcacgccg cttgcgccct cctaaggaac
      301 gatcacatcg ctagggctct ggcagatgag gcatgtaagg tattgatgcc tcaactctgc
      361 tagactccct cgctcatgcg ttacacgcag gcatttctac ccacgctcaa agaagatcca
      421 gccgatgcgg aactcgtcag ccacaagctg atggtccggg gcggcatgct gcggaagctc
      481 gcggcgggca tctatacgat cctgccgctc ggctggcgcg tcacgtccaa ggtcgcgcgc
      541 atcgtgcgcg aggagatgga ccgcgcgggg tgccaggagg tcctgctccc gatcctcatg
      601 ccggcggagc tctggaagga atcggggcgc tgggacaagt acgggccgga gctcttccgt
      661 cagcgggacc gccaccagcg cgacttcgcg ttggggccga cgcacgagga ggcgatcacc
      721 gatctcgtgc ggaaccacgt ccgctcgtat cgcgacctgc cgctcaacct ctaccagatc
      781 cagaccaagt ttcgggacga gatccgcccg cgattcggcg tcatgcgcgc gcgcgagttc
      841 atgatgaagg acgggtacag tttccacgtg gacgaggcgt cgctcgggca gggctacgag
      901 cgcatgcacg aggcgtactc ggccgtgttt cgccggtgcg gactcgagtt cgttgtggtg
      961 gaggcggact cgggtgcgat cggcggggac gtgaaccacg agttcatggt cacggccgac
     1021 tccggcgagt cgatggtgtt ctcctcggcc tgcggctacg ctgcgtcgga ggagagcgcc
     1081 aagtacgccg tgccggagca tgcggccgaa gcggcccagg cgctcgcccc gaaggacacg
     1141 ccggggaaga agagcgtcga ggaagtggcc gagttcctca aggtcacccc cgaccgcctc
     1201 ctcaagagcc tcgtcttcat cgcgggcgac gatccggtgc tcgccgtcgt gccgggagac
     1261 cgcgagctga acgaagccaa gctcgcccgg gcgctggggg gcgttcccat cagactcgca
     1321 accgcacagg aaatcgagaa gatcacgggc ggcccgctag ggttcacggg cccggtgggt
     1381 ctcgagtcca aggtccacac cctctacgac gcgtccctcg gaaacagccg caactacgtg
     1441 accggcggaa acaagctcga cgtccatctc accaacgtcc gcttcgggcg cgacctcacg
     1501 ggcggcaccc aggcggatct ctcgaccgcg cgcgagggag acaggtgccc gcgctgcggc
     1561 gacgtgatgc gcgtgacgcg gggcatcgag gtggggcaca tcttcaagct cggacttcgc
     1621 tactcggagc cgatgaaggc gaccttcctc gatccggaag gggtagagcg gacgatcgtc
     1681 atggggacct acggcatcgg cgtcacgcgc accgtggccg cggtcatcga gcagctccac
     1741 gacgacaatg ggatgatctg gccgatctcg atcgcgccgt accaggtcca catcgtggcc
     1801 gtgaacgtga aggacgacac cacacgcggc gccgcggatc agctctacgc ggcgctacag
     1861 gagcgcggga tcgaggtcct ctacgacgat cgcgacgagc ggccgggggc gaagttcaag
     1921 gacgccgacc tgctcggcat tccgctccgc gtgacggtgg gggagaaggg gctgaaggaa
     1981 gggatcgtcg agcttcggga ccggaggacg ggtgtcgtcg agaagctgcc cgtccaggac
     2041 gcggccgacg ccgtggcgcg gcgcgtccgg gacgacctta cgaagctttc gtagtcttcg
     2101 cagcggcgcg attcgccatc ttcgcgagcc gcgacttccg acgattggca accgccttgg
     2161 ggatcacgcc cttccggacc gcccggtcca gccgggacgc cgtcatgcgc gcaagttctt
     2221 tgtccccagg ggtttcctgc atcttcttca ggtaggtttt gatctcggac ttgacggaag
     2281 cgttcttcgt cgccgaccgc ttggccgtgc gcatacgctt gaccgccgac ttgtgatgcg
     2341 gcattcagct cctccggaac gggtgacagc tcgaacgtgc gaagtgcgcg actctacggg
     2401 acttccggcg tcctgtcaag cgaggccggc gtggtatact cgcccgaatg gcaccgcgac
     2461 tcccgaaacg ccgcggcgac gctctccccc ctgatccccc cgacgatgta gccaaggcgg
     2521 acctgaagca agcgatcgcc gctctccccg ctcagcccgg ggtctatctg ttccgcgacc
     2581 ggaacgggca gctcctctac gtcgggaaag cgaagcgtct ggacgcgcgc gtgcggtcgc
     2641 atttccagga tccggaggcg ctcggcccga ggcaggtcgt cctcacgcgg cggacacagg
     2701 ccgtggagta catcgcggtg gactccgaga tcgacgcgct gctcctggag gccacgctcg
     2761 tccgggagcg ccagccgccg ttcaacgtcc ggctgaagga cgacaagcgg tacccctatc
     2821 ttcgcgtgtc gtggcaggag ccgtttccgc ggctcacggt cgtgcgcaag gtggagaagg
     2881 atggggccag gtacttcggc ccctacaccg aggtgaagag cctgcgggcg ctcctccgcc
     2941 tcacgcagac gatctttccg atgcgcacct gcacggacat cgacgcgcac atccaggcca
     3001 agcgtgaatg cctcgagttc cacatcggcc gctgcacggg accctgcatc gcggcgcagt
     3061 cggccgagga ctatcgcgcg atggtggatc agttctgcga cttcctcgcg ggcaagcggg
     3121 aggaagtggt ggctcgcctc cgcgcgcttc aagagaacgc ggcggagcgg cgggaatacg
     3181 agcgcgcggc tcgcctccgc gaccagatca agaagctcga gcagatcctg gcacggcagc
     3241 ggatggtcga catcgcgggc ctgtcggctg acgttctcgg cctcgcgcgc gaaggggagc
     3301 gatgctgcgt cgtcgtgctc aaggtgcgcg agcgccgcgt cgtcgccaag gacgtgcggt
     3361 ggctccgcgg cacgagtggg aagaccgaag gggagctgct ccgtgcgttc gtcacgctct
     3421 actactcgca aaccgaggcg attcccgagg cgctcgtggt cgagaccgat ccggccgagt
     3481 gcgagcttct cgtgggctac ctcgagcgcg gcgcgggggg ccctgtgcgc gtccggcagc
     3541 cgcgggatgg aaccgagcgg gcgctcctcc ggaccgcgca tcggaacgcc acgatgctcg
     3601 tgtcgcgggc gcgcgaagcc gcggggaagg gactcgaggg gaacgcggcc gacgaagcgc
     3661 tcgagctgca gcgcgctctg gggatcgacc gacttccgcg gcggatccgc tgcttcgacg
     3721 tgtcgcagct cttcggtacg tatgtcgtcg cgtcgatggt gacgttcatc gacgggcgtc
     3781 cctttaaaaa cgagtaccgc cactatcgaa tccggaccgt cgaggggcag gacgacttcg
     3841 cctccatcgc cgaggcggtg cgtcgccacg ccggacgcgt gaccgaaggg gatatggagg
     3901 cggcggatct cgtgctcatc gacggaggga tcgggcagct ccaggcggcg ctggccgccg
     3961 tccaggggac gccgctcgaa tccgtgccgt tcgccgggct cgcgaagcgg ctcgaggaga
     4021 tcgtgcggcc ggggggcgcg ccggtcctca ggctctccag gcgctcgccc gcgctcaagc
     4081 tgctcatgcg ggtccgcgac gaggcgcatc gattcgcgat cgtctaccat cgcacgcttc
     4141 gtgggcgggc ggttcggagc acggagctcg agaaggtgcc cgggctcggt cccaagcggc
     4201 aggcgctcct cctccggcgc ttcggctccg tggagcgcct caagcagcag ccggtggacg
     4261 ccatcgcggc ggtgccgggc atcggcgagc ggatggcggc gaggattctc gaggtcgttg
     4321 gaagcgcggg aggaccccag tgagcagctc gacccagcat gcgcgaagcg cttcgccgcg
     4381 gagcgccacg aaacgaacga gcacgcggct tgctcgccgc aaggccgagc gcgagccggt
     4441 ggccgccgcc agcgtcaaga cgccgctcgg caagatctgg atcctcgcca ccgaatcggg
     4501 tctgcgcgag atccgtctcg ccgaccaagg ccgccccacg cgccccgaga tggatgcgcg
     4561 cgggtggaag ctggtccgga agaagaagtg gaccgacccc gttcgccgga tcctcgagcg
     4621 ctatctccag ggccgcgagc agcgcctcga gatcgagctc gatctcgcga tcgggactcc
     4681 gttccagcgc cgcgtgtggg atgccgcgcg ccggatcccg tacggcgcgg tcgtctccta
     4741 cgccgagctc gcgtcgatgg ccggagggtc gggggcgcag cgcgccgtcg ggaacgcgct
     4801 cggcgcgaat cccgtgccga tcgtggtgcc gtgccaccgg atcatccacg ccgatcagtc
     4861 gatcggcgga ttctcgtcgg ggcttcgctg gaagcgacac ctgctcacca tcgagcgagg
     4921 tcagctcgag ctcacctgga cgaaaccgcg gcggtcgctc ttcggccgat gacgcgtccc
     4981 gcgaagagcg cgtcccagcc cgcgacaacc ccgctcgggg ccgacctcga gcgcttccgc
     5041 gaacacctgg cgctcgagcg ccgcatgagc gagcacaccg tgcgcgccta cgtccgagac
     5101 ctggagcagt acggacgctt cctcggcgga cggaagatcc ggaccgccgg ctcggccagc
     5161 gcgcgcgaca tcgaggagta catgcgggag gcgaagtggg ccgcgtccac agtggcgcgc
     5221 aagctggccg ccctgcgcgc ttttcacgag ttcttgcgcc ggcgcggcgt cgcgggagac
     5281 aatcccgcgc ttcaggttcg cccgccgcgt cgctcgcggc cgttgccgga cgtgctgtcc
     5341 gtgccccagg tggaggcgct cctcaaggcc ccgcgggggg aggagccggc ccagatccgg
     5401 gaccgggcga tgctggaggt ggcgtacgcg accgggctcc gcgtgacgga gctcgtgaag
     5461 ctccggatcg aggagatcga cggcgaggag cagctcgtac gctgcatggg gaagcgctcg
     5521 cgggagcgga tcgtgccgta cgggaatcag gcgaagcgcg cgctcgagga gtacctcgac
     5581 cgcgcgcgtc ccggcctggt gcgggaccgg agcgagcgcg cggtgttcct gacgcgcctc
     5641 ggacggtcgt tcagccggat gggtttctgg aagcttctca agggttacgc caaggcggcg
     5701 gggatcgagc agccggtctc cccgcacacg ctccgccact cgtgcgcgac gcacctgctc
     5761 gaggggggct gcgatcttcg cgtggtgcag gagatcctgg ggcaccgttc catcgagacg
     5821 acgcagatct atacgcatct cgatcgcggc tacctgcgcg aggtgcatac gcggtttcat
     5881 ccccgggcct gagggcccaa gccggaacgt ggaggactgg atgacgagga tcggggatcg
     5941 acccgggcga ggcggctggg cgctcgggct cgcgatgttt gccgtgctgt ccatcatggc
     6001 gctcggcaac gcgcgcgccc aagagtccga tcccaacggg ctcgaccccg atgcgcagat
     6061 cgcgctcggc cccatccgct atgcatcgtc cgtctcgctc ggcatgcgcc agccacctcc
     6121 gacgcaggag cggccacggg ccccgctcct gcggcgccag cgcccgaaca cgatctcgat
     6181 cggcatccag ggggactacg ggatcgtccg cggcaactcg cgactcgcgg atggcttcga
     6241 cgacggccct gggtattcat tccgattccg ctacatgctg gccccgtcgt tcgccctcgg
     6301 cttctcgttc gagagccagc gcttctacga caagggaggc atcccctcga ccgagattgg
     6361 ggcgggggac agcacggtcg tcatgacgac cgtgggcgcg gagggcgtgt tctacatcca
     6421 tcgcgagcgc gaggtgaatc cctacttcgt gaccggcttc ggctacgcga gcccggacgt
     6481 cgtcgacgag gtgcgcggca gcgcccgcgt gaacgagggc atgttcctcg tgctcgggag
     6541 cggtctcgag cgattcatcc ggccccgctt ctcgctcgac ttcacgctcc gcggctacgg
     6601 gctgatcagc aattccgagt tcacaggcat cgccacgctc agtgcgggca tccatctcta
     6661 tcccggagac tagtcccgca tgcgggtgac gttctggggc gcggcccgaa ccgtcacggg
     6721 ctccaagcat ctcctgaccg gcgagagcgg aaccgtcctc ctggactgcg gcctgttcca
     6781 gggacgccgc gccgagtccg aagccaggaa tcgcgcgctt ccgttcaacg gcgccgccat
     6841 cgacgcggtg gtgctctcgc acgcgcacat cgaccactcc ggcgcgcttc cggtgctctg
     6901 ccggagcgga ttcgacggaa agatccacgc gacgcgcacg accgtcgatc tctgccgtcc
     6961 gatgctcctc gatgccgccc acctccagat gaaggacgcc gagttcatga acaagcggcg
     7021 gaatcacaac cacaaggggg atcccgtggt ccccctgtat accgccgggg acgtggagtg
     7081 cgtcctcgag cgcatggtgg gggaggagta cgagacgcca ttcgagccga tcccgggcgt
     7141 gtccgtgatg ttccacgagg cggggcacat tgcgggcgcg gcgtcggtgt cgcttcgctg
     7201 gaaggagaac gggcgcgagg gatcgctcgt cttctccggg gacctgggcc gccccggccg
     7261 ccccatcctg aaagatccgg tgccgctgca gaaagccgac tacctcatca ccgaagggac
     7321 gtatggggga aagcaccatc ccgacgagga gacgctctgg acctcgttcg aggcggtgct
     7381 gaaggacgcg atcgcgcgcg gggggcgcat cgtgattccg gcgttcgccg tggggcggac
     7441 ccaggaggtg gtctacgcgc tcgaccacct gttccgctcc gggcgcgtgc ccgagatgcc
     7501 gatctacatc gacacgccgc tcgggatcga ggcccaaacc atcatccgga gccatccgga
     7561 ggtgttcgat gaggagaccg cggcggcgct ccgcgcgggt ggcgatccct ttggcatgca
     7621 gcgcgccacg ctgatcaagg atgcggagga ctcgaagaag ctcaacgaca aaccgggcac
     7681 cttcatcacg gtctcggcgt cgggaatgtg cgagggaggg cgaatcctcc atcacctgaa
     7741 gcaaacagtc ggcgacgcca agcatacgat cgtcatcatc ggttatcagg ctgagggaac
     7801 gctggggcgc cgcctcgtcg agcacgtccc ggaggtgaag atcttcggcg aaccctacga
     7861 agtccatgcg aaggtcgtcg tgctgaacgg attcagcgcg cacgccgatc atgacgcgct
     7921 cgtggcgcag gcggccgcgt gcggcgcacc gaagggtgtc gcgatcgtgc acgccgacct
     7981 cgagcgggcc gaggccctga aggccggcct cccgtcgccg gagcgcgtcc gcatccccac
     8041 cgagggacat acctgggagc tcgcgtgacc cgcgaggagc ggcgcggccg ctaccacggt
     8101 tttcgcgtcg gcgaccgggt cctccacaag gaattcggcg aagggctcat cgtcgaggtc
     8161 cgcgatcggg acggattcga ggttctcgag attgcgttcc cggacggaat ccgccggctc
     8221 acctcgctct atccgctcga gcccgcgcgc ggcgcgggac gcgcgacctc ggcgccggcc
     8281 gtgtcggcgg cgacgtccgc gccggccctc cggctcggca ccgatctccc cgcgcgcggg
     8341 cagttccgga tcgctccggg cgcggacgag ctcgtccagc ggctcgtcgc gggcc
//
LOCUS       DASTKC010000183        28341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_386143, whole genome shotgun sequence.
ACCESSION   DASTKC010000183 DASTKC010000000
VERSION     DASTKC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 28341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28341
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386143"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="VFR25_07455"
     CDS             complement(<1..1869)
                     /locus_tag="VFR25_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9940922.1"
                     /translation="MSRIARSYLIGGALLTFIAAGPAVPSAAARTRPEPHAAFTAPSP
                     RAPDVLSAQSLARLKAEGRSVLWVHFTDKAIPDAGSFAASLRSVDDRMDPRARARRAR
                     ETGGRFVPDYYDLPVAQNYVDAVAATGVDVRHVSRWLNAMTVIANEGQARRIAALPFV
                     RRVTPARVSRRIEAVGVAPGAPQAPSEQGFLRDAGPRPGDLLAGTVAGQFTALPKPFS
                     YGGSITALNGINVPAAHDSGWSGASVIVAMFDTGYDKAHPSLTLLQKIAERDFIFHDG
                     ETANQAGDVTGQWDHGTGTWSVLGGYASSNLVGPAFNARFVLAKTEDVRSETPVEEDN
                     WVAAAEWADSIGVDVISSSLAYLDFDGTANDYQYTDLDGYTTVVALGAIFAARRGIVV
                     CNAMGNEGGAAGSLWSPADADSILSVGAVSDMNFIAAFSGRGPTADGRMKPEVVAQGI
                     NVQWAVAGATTYALASGTSLSTPLIGGAAALVREAHPEWTVAQVRDALMQTADKAATP
                     DNDFGHGRINVVKAIYGSSYGVPVAPNPFNLRVPINNGSVTQVPVTFRWNKSIDPQAG
                     AVHYRLDLRVVAPQPCCIFSTTTPDTFLVYNGYLGPSRTYEWTVTAIDPQSHERLSK"
     gene            complement(1925..4015)
                     /gene="fusA"
                     /locus_tag="VFR25_07460"
     CDS             complement(1925..4015)
                     /gene="fusA"
                     /locus_tag="VFR25_07460"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9940923.1"
                     /translation="MKDFQPSQIRNIALVSQHGTGKTSLAESLLFRTKAITRHGKIEE
                     GTTALDFTPEEIHRKITISLALAPVEWRGTKINLIDTPGYADFIGDCVAGLCAADAAL
                     FCLRGAAGVEASTEAIWERVHERNCPVLCAVTMMEKEHASFSKVVSSASERLGIRFVP
                     VAWPIGEADQFRGVVDLASMKALIFEKDGSHKEAPIPAELEADVKKARSALVDAAAEA
                     DDQLLEKFLGGEELSVEEIHTGLREGVAHRSFVPAVPVAAVPMLGIDFLLDTLVDLAP
                     SPVDMGAVHAKKAGGSEEEDLKPELAAHLSALVFKTSSESHAGDLSMIRVFSGNFTPG
                     KEVWNSSAQRAEKIGQVFTIKGKDRTDMGHIAAGDIGAAVKLRETHTGQTLCEKSHPV
                     TLAPIPFPSSEHEVAIAPKSKGEEEKMGSGLHKLREEDPTIHVRVASDLHQTILSSMG
                     DLQTEVVIEKLNRRFGVHVETAKPRIPYRETIRKSVSKQGRHKKQTGGRGQFGDVHVR
                     LEPMKNGEGFEFVDEVVGGAVPRNFIPAVEKGIVEAMHEGVLAGYPVVGVRAALYDGS
                     YHSVDSSEMAFKIAGSMAFKEGAKEAGPVLLEPIVEVEVKVPKDFVGAVTGDLSSKRG
                     KILGMGSEGRYDVIRAHVPQAELYKYSTHLRSLTQGRASHHTKFAHYEEVPRELADRI
                     VAQARADKEAMASA"
     gene            complement(4193..5026)
                     /locus_tag="VFR25_07465"
     CDS             complement(4193..5026)
                     /locus_tag="VFR25_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET9940924.1"
                     /translation="MIRGAVLLAAVVALLGASTANAAFPPIDLTTAGTTVTSAVDGTI
                     WTNTNLIQSSGTGVIEPFLRWQANNTETGMNTDVPPPYDTKAGIWTHSITLGQLGIKN
                     VSGIDYYVFTLDINEPNQDAAFLTLNEMRIYTSSAGGSLADEPAVIANGTLRYNLDGT
                     SDQTIYTDYRVSNTGSGESDVDFYIPTSFFAGAAASDNLYFVVNSGLSGNTDGLDSAD
                     GFEEIRAFFGTTSSSSTTSSSTTTSSSSTTSTTSTAPEPGTIALFGMGLIGLAAARRK
                     K"
     gene            complement(5188..6765)
                     /locus_tag="VFR25_07470"
     CDS             complement(5188..6765)
                     /locus_tag="VFR25_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940925.1"
                     /translation="MNRLGGTTRVARSWLALLALALVLSGCGDTSLRATRYRAEQLLW
                     HADLQESAVRLRDENPDSTTLLGLRAPYLDVAKKVKIPPLSAARSEEESTLSIDVMRL
                     VGNADLQAARLSVQANRPDLGIESMKRVRAMAGNDTLMLRRADFFLVGTLRQYKRYEE
                     AVALMKTMLERYPPISPQGTQDEDAVLAIPESIVRLYRDLNDSTRANAALNDAGAYYR
                     RVLATPHSPILDAQVRARLIRVELEQGDWSEGLNDVAILRRLAAASPSLKDLEPEIRY
                     SEAKLHMMHTGKENPNESVALFEKVAKDFPKSPFAGRALMDAATLLELHSRFPEALNY
                     YRQVTTSYGNQPDIAPPAFFRRAMLEDKLGNWEQAKNLLDGIPVRYPETRAALEAPIA
                     IANRYVRIGQADAAKQAVRKAILTYQGLIERDTTSGYTTFYRWSIARCYALLGDQQGV
                     LRQIDEMVRRDMGHPIMEQALLQGAQVAHQAKQDDRARAYLQNLLVNYPRSPFVPDVK
                     KQLARIPARGTAAKTGS"
     gene            complement(6762..7604)
                     /locus_tag="VFR25_07475"
     CDS             complement(6762..7604)
                     /locus_tag="VFR25_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940926.1"
                     /translation="MRRDFRVARACGVEAARDFQWHEYAPFAWILGAQLLFLLLALNL
                     GTTISMGTAGAAVKLFAGDAPLHYPTFFLYLPNAASWWELILYTVAGAYLIPLSIARV
                     QTPMDPDLQGPGFKARVNRAWPPTLIAGLLTFALLSGWQWLFKVALVPLIRASFPGLQ
                     ATAAIWGLSLVVSYSLSALFIYVPILALQPGSSLVKALREGIREGVRLFKWTVLYLFV
                     FALPALPFLMAVQLGTAFLATRMRPEMIAVVLAIYAILISVATYLTYAAAARLHWASQ
                     VEEA"
     gene            7662..9344
                     /locus_tag="VFR25_07480"
     CDS             7662..9344
                     /locus_tag="VFR25_07480"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9940927.1"
                     /translation="MATDDYQIQIRRPGAEPEVFLLDKNAPCTLGRSPQCRVFLSDAS
                     ISRNHAEIFWRDGSFWIQDLGSKNGTKLGSKKIEGPHPLKPGDQLHLGSVHLSFNKAG
                     EAPSHETSVSARVADIAAPVNVHAVPLEDISSGSVALDTRSFGSSLSPERVGNFLQAM
                     DRVGQALLAYRPLDELFQFVVGLAADVLRADRTAILLRRASDDDLVTMAVSQSGRGAG
                     EEIIVSRSIARMAVGQRQAILTSDAQSDTRFKEQQSVIRQRIHSAMCAPLWHDGEVLG
                     LLYVDNVAAPVPFEEGDLRLLTFIAHLAAVKIRETESHEKDLEREEELRRAAALQQAL
                     LPHEPRVAGVIEVAGRNVPSLNVGGDYFDFVDGGENRMVVGLGDVAGKGMPAALLMTH
                     LAASVRAQVETERPLLEVMSRLNRSIYQNVRGERFITLVLAEIDTTDGSILYVNGGHN
                     PPLLLRSGTNEIEMLSEGGLLLGIMPEAKYAQGTVTMGPGDILLFYSDGVTEARNLAE
                     EEFGEDRLMTFIREARALTPAQMVETLIERVRDFSRRPKPTDDVTVVAVRRA"
     gene            complement(9367..10287)
                     /locus_tag="VFR25_07485"
     CDS             complement(9367..10287)
                     /locus_tag="VFR25_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940928.1"
                     /translation="MTLGSVSSLGVSGQRLETRAGRAAPPATGTAAVSNAAGPSSSIS
                     SDTGEAPLSESEGIQRELTHTDRVLAVLKPKVYRTGNTQAQSRFGEAVTREKEARDAF
                     SRNLYARATRLTREARSLAREAAVMVGPPEDDPVCVGRAIDNAEDALSLARDVFDQGA
                     NPEVWRRYHALRDSLQESRQLLESGDARVAYRRAMQVRDGVLDLLQDSQNLPVPAPTA
                     ERALRQAQDAYDRATRELGANPKESALKWRREAQGQLAKAKSAFHHRDYRDSILHAKL
                     VERSLDEAIAAQRNAELRSNGGVRTARRSA"
     gene            complement(10590..11987)
                     /locus_tag="VFR25_07490"
     CDS             complement(10590..11987)
                     /locus_tag="VFR25_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9940929.1"
                     /translation="MPERILVAEDKENLCRLIETTLSEQGYDVSIALGGDAAIHEIQE
                     HTFDLVISDMRMPGASGVQVFREARQQGYLPDVILMTAFADTREAVQMMKEGATDYIM
                     KDNLLEELPVRVRQVLDHRKLRQESTVLKTRIESLREQIPIHRFDSIVGTSLPMRDAR
                     SLAERVAPTDANVLLTGESGTGKEVFAQAIHAASHRSDAPFVPVNCGALPETLLESEL
                     FGHEKGAFTGAIRTKPGRFEVAEGGTVFLDEIGELPQSIQVKLLRVLQDRTYVRVGGE
                     DMRRADVRILAATNRDLEEMIHHGRFREDLYYRLNVFPIRLVALRERREDIPSLVGHF
                     LIMQERTIDELTPDALEYLRNYDFPGNIRELQNLIERACILAGRGGRIERHHFPLGRA
                     RQAADPADLLSLGLSLEQIEKRLIKEALDRAQGNKTKAATLLGISRRALYSRMESHGI
                     PIGGPEPEGSEAPAF"
     gene            complement(12032..13414)
                     /locus_tag="VFR25_07495"
     CDS             complement(12032..13414)
                     /locus_tag="VFR25_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9940930.1"
                     /translation="MFPRRQNIRPRIVWTLAFLIALLVLVNGAIFFSLKTSQKLLDQE
                     LGKRLQGTAHIAALLVAPEQFEMLSLAGADTSAAADTALTDFSMKMDSQEAADAVRAE
                     WTRLAQSAGLSNVVLVDRHRRVLLRLRDPFTYEPDVVTLDPVPMTKALIGQASHSRLY
                     YKDGQYLRSGYAPVLTPEGGVVGAVAVEGGSEAFQPLQRIRASVAGVVVLSSVLVILI
                     GLGFVRTVGHLARIEENLRHTDILASIGQVSAGVAHEIRNPLAVLQGASSRLKRRETQ
                     PKEQAELLSMIDEEVNRMSAFVNNFLHLSRRPNLEPQEFELRPVLERSLEMLRVELDR
                     ASVSTVLEWHAGGSVVLIGDPLAMHHVFLNLALNARDVMPSGGKLVIRVSEKRGELRI
                     QFEDTGPGVPKDLRKKIFDAFFTTRAKGTGLGLAFVDRIVSEHGGSVTVGDAPKGGAM
                     FEVRLPLEGP"
     gene            13532..14323
                     /locus_tag="VFR25_07500"
     CDS             13532..14323
                     /locus_tag="VFR25_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9940931.1"
                     /translation="MNRTLLLFAFGLLAGALGGLMGVGGGLVLVPLLVRFLGMGQHEA
                     QGTSLAFIVSAALVASAKYYGSDRLDVPLALTIAAGAVPGVLLGSALAARTSGRTLRL
                     CFGVLIAITAIRILAFPPVHERAGSLWPAAANVALGLGMGTLAGLLGVGGGILVVPSL
                     VLGEGVGQHEAQGVSLLVIVPVAIAGVISYAARGRIARGSIPATMAGGAAGGFLGALA
                     AHHIRGETLSRIFGAFLLVVAAQMIFRRARGTVPRSLGAESGGIP"
     gene            14320..16044
                     /locus_tag="VFR25_07505"
     CDS             14320..16044
                     /locus_tag="VFR25_07505"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET9940932.1"
                     /translation="MTTKTRFALFPILALTLSLACASQPSPQEKAAFETLARRYLMAG
                     YAFNPSFATAWGIHDYDDSLENWTPERIHSEEQRVRDAMKELDAIRPEKLDPATRIDY
                     DLLRTGMNGNLNYLTELRGWENDPGQYNYGGTLLSMMTVDYEPLPERMRSLTRRLEQV
                     PRQLANARLNLKNPPQMFTQFAMGDFEGTISFLENDVTRAFASVTDPTLRAEFDAAKK
                     TAIAATKDHIAWMKSMLLPASHGSYVLGEDKYRKRLRYEEMIDLPIDTLLAVGQRELD
                     RLEARYKEALQRVDPSATQEAIVARMRADHPPADSILDYARALCEEARAFTIQSGFIK
                     IPDNAHITVRPTPEFAASRSFASFDAPGPFETKADDAYYNITLPDPKWPKQQIEEHLQ
                     GFNRWTLPSVSVHEVYPGHYVHHLYGKRAPSMVRKSIGSGAFAEGWGLYTEEGLLDEG
                     FRKDDPRVEVGVMRWALVRACRLQVGIRVHTRGMSLEDGTKFFMEHAGMERANAERES
                     GRAAFDPTYSVYTLGALEIRKLRDDYKREQGANFNLGQFHETILSQGALPVALLRKML
                     LKHEGTIL"
     gene            16101..17087
                     /locus_tag="VFR25_07510"
     CDS             16101..17087
                     /locus_tag="VFR25_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET9940933.1"
                     /translation="MPSREVEALRRDFPDVDVIDTRTREELDAAVPEADILFSWAFGD
                     EVIARAKRLRWLHVPAAGVGSYLTPSFRASGAVITNSRGAHAIPIAEHVMAGLLGIAR
                     RMKAGIVEQTTTGMIRENWWAEDQVPDELHGKTIGLFGYGAIGREVARRAVSFGMRVI
                     ALRRRPHGAPAWDPELLQALDLPAEEPPVAETFGPGDLDRFLGRSDVVVVSAALTQET
                     EGALGRAQFARMKPGAWLVNIARGKIVQEAELVTAVREGRIRAAALDVYEREPLPRES
                     PLYTLDRVILTPHVSGLSRGFWPRAMALFRANLRRDARGEPLLNRVDPDLGY"
     gene            17096..17824
                     /locus_tag="VFR25_07515"
     CDS             17096..17824
                     /locus_tag="VFR25_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9940934.1"
                     /translation="MPRPLIGIGPNYRLKQRRGDSYVLDANYMKLIGGAGGLPLILPL
                     VSTLEEAREVVGRVDGLVLSGGSDLESERYGQGTRHPDRLGAPARNASDLFLARAAQE
                     RGIPTLGICLGIQVMNVEFGGSLLQHLDEDRPGGMKHEEETDDETPEHTVHVEPGTTL
                     AKVLGQERVVVNSYHHQSVDRIAPGFRIAATSEDGVVEAIERPDHPFYVGVQWHPERM
                     PDALATRRLVGAFVDAARGASIRS"
     gene            complement(17861..18463)
                     /locus_tag="VFR25_07520"
     CDS             complement(17861..18463)
                     /locus_tag="VFR25_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9940935.1"
                     /translation="MKKSTRKGSRGVRAGRARNRAPKRAARRVRITPEIRAYVRKIDA
                     YRGGRAPLPLMRVGPAKIARAIQGLRSAQLKKRPAPGKWSIAEILGHLVDTEVVYGYR
                     YRLALSQPGVPIPGYDQATWTVALKHRNRNPARLMAQIATLRKINLDLIESMPRRTWE
                     RYGMHSERGKETVRRTAELIAGHDLNHLDQIQTIKKKYGW"
     gene            18599..19558
                     /locus_tag="VFR25_07525"
     CDS             18599..19558
                     /locus_tag="VFR25_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940936.1"
                     /translation="MRRPPFPLAPALLLAALFLGASTALADSEKAETFHIMEIVQVMA
                     AYNGNTAIQAVEMKELAAGQDLVASGQINIYDKDGVLGTTLGTFAANVANGASGAHIL
                     CATAAFASAFSITPDLVLSPGIPLPSGQVVFEKVGCKVNSVPYGGISTIVANTSVAPT
                     IPTLGATALVRTGDAPTITTCPLTDSAASRFAIRSGTSASSFLVFQTNAGDTAHVWST
                     ATGVGTTPPEPVRLQASPNPFRAETRIDAPDWGPLAIHDVQGRLVRVLTCAPGGACPQ
                     VAGAFRGSWDGRDERGRAMPSGVYYLRYSGASGHAVKAIVYLR"
     gene            complement(19574..19897)
                     /locus_tag="VFR25_07530"
     CDS             complement(19574..19897)
                     /locus_tag="VFR25_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9940937.1"
                     /translation="MRRAIAISILVLAAAGCGGGKAEIPVSVTENGFEPSRIEVKHGE
                     AVTLLVTRKTDVTCATEMVVPSRGITQELPLNQTVRVALGPLKPGEVAFACGMDMEKG
                     TIVVR"
     gene            complement(19927..22212)
                     /locus_tag="VFR25_07535"
     CDS             complement(19927..22212)
                     /locus_tag="VFR25_07535"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9940938.1"
                     /translation="MRVAPERAAGRYDYKGATYYFCSPGCLAKFRADPERYLAPGAKH
                     EAMTHDPTPPASSAGTQWTCPMHPEVIRDRPGSCPICGMALEPMTVSLDDEENPEYAD
                     MRRRFWISVALSVPLIAFAIADLIPARPLEGLAHVRHWLELAFATPVVLWCGWPLLAR
                     GWDSIRHRALNMFTLIALGVSVSYAYSVVAVLAPGIFPAALRDAHGLVPIYFEVTVMI
                     TTLVLLGQVLELSARSRTGAAIRQLLRLAPKTARRIGPDGGEHDVPLEAVLVGDSLRV
                     RPGETIPVDGVVLEGASSVDESMVSGEPIPVEKSAGSLVIGATVNGTGSLVMRAERVG
                     SETLLARIVRMVSEAQRSRAPIQRLADTVSSYFVPIVLAVAVVTFLVWMLAGPEPRLA
                     HAIVNAVAVLIIACPCALGLATPMAVMVAAGQGATAGVLFRNAQAIETLRDVDTLVVD
                     KTGTLTEGKPRLASVVATDGWSDDEIVRLAASLERASEHPLAHAILAGALERRLQIAD
                     VREFESMSGMGVAGIVEGKRVALGNRKHMDDLGVRGDDLWEQAEALRVEGRTVVAVAV
                     EGRLAGLLAAADPIKPTTKEAIQALHADGIKIVMLTGDSAATANAVARSLGIDEVVAE
                     VLPDQKLEFVKRYQAEGRFVAMAGDGINDAPALAQAQVGIAMATGTDVAMESADITLI
                     RGDLRGIVRARRLSRATMGVIRQNLFFAFVYNVLGVPIAAGVLYPVFGLLLSPIIAAA
                     AMSLSSVSVIWNSNRLRGVRL"
     gene            complement(22295..22630)
                     /locus_tag="VFR25_07540"
     CDS             complement(22295..22630)
                     /locus_tag="VFR25_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9940939.1"
                     /translation="MKGRIIGIVALALLGGVWGCSEKNSQTIRMTVSDRGFVPAEITV
                     KRGVPVTLLVTRETAATCATELVIQGAGVRKELPLHQEVSVTFTPKTKGELRYTCPMD
                     MVSGSIQVE"
     gene            complement(22642..25782)
                     /locus_tag="VFR25_07545"
     CDS             complement(22642..25782)
                     /locus_tag="VFR25_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET9940940.1"
                     /translation="MIERLITWSARNRILTIALVLVAAAAGLRALRHTPLDAIPDLSD
                     TQVIVSTEWTGRSPDLVEDQVTYPIVTALRSAPGVRYVRGLSMVGDSFVYVVFEDGVD
                     LYWARSRVLEYLASLQGKLPEGVTPRLGPDATSVGWVFEYALVDTTGQNDLSQLRSFQ
                     DWHLRYWLQSVDGVAEVAAVGGFVRQYQVDLDPRRLESYGVSIREVANAIQVSNKDVG
                     GSVLEVGEHENFVRGRGYIRSLDDLRGIVVKTGEGGVPVTVAQLGEVYLGPEPRRGIA
                     ELNGQGEVVGGIVIMRQKENALHVIDGVKRRLAEVEGSFPPGVRLVTTYDRSELIRAA
                     VHTLWRSLLEEMAIVSLVIVAFLFHFRSALVPILTLPIAVVLSFLPMSAQGLTANIMS
                     LGGIAVAIGAMVDASIIMVENVHKKLEAWEAGGRAGSRDEAILAGLREVGRPVFFALM
                     VITISFLPIFTLEGTEGRLFKPLAYTKTYSMFFAALLAVTLTPALAAWFIRGRIRSER
                     EHPLSRRMVSAYGPVVRFAVRRRGWVVGAALLLVLSTIPIFFRLGSEFMPPLNEGTIL
                     YMPTAPPGISETEAATVLQLMDRRLREVPEVQTVFGKVGRARTATDPAPLSMVETVVT
                     LKPESEWRKGMTWDKLVAEMDARSRFPGMPNLWWMPIQTRNEMLATGVRSAVGVKVFG
                     SDLATIERIGIEVEHALQGVKGTRAAFAERVTGGYFLDFDIDRAAAARYGLRVGDVQD
                     VIEAAIGGMTVSQSVEGRERYPISVRYAREFRDDPDALARVLIPTPSGGQVPLAQVAR
                     IQFRTGPPMLVEEDGQLFGLVSVDVANRPLASYVEDARRAVEGKVSLPPGYRLEWSGQ
                     FQYYERARARLLLVVPVTLLLVFGLLYFNLRSVTETMIVMLAVPFSLVGAVWILWLLG
                     YNMSVAVWVGMIALAGLDAETGVVMLLYLNLAHRDRAERGALRDRADLEDAIVEGAAH
                     RIRPKMMTVCAILFGLLPILWGHGSGADIMRRIAAPMIGGVLTSFVLELVVYPAIYAW
                     WKGRHLPARAGAPGPA"
     gene            complement(25790..27253)
                     /locus_tag="VFR25_07550"
     CDS             complement(25790..27253)
                     /locus_tag="VFR25_07550"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9940941.1"
                     /translation="MSRRWVLIGIALVALAGCSKHETKTSRSTETAAAATQRVGAFQI
                     SLAARPERPVVGDNAFKVALFDSAGRPLSGASLAAIVSMPAMGAMPRMESRGEFRETG
                     AGIYEASYGLQMQGDWDVAISIHVSGSPDAEAIYRVSTNMTGASFVGGSPAAGAPAAL
                     DANAATLGAEVIIDPARRQAIGIRTEPATVQDLKATIRAAGRVTYDETRRAEISLKFS
                     GWIREIHVDYVGRQVSRGEVLFTVYSPELLSAQQEYLEARRDPMGGDLTAAARQRLLL
                     WDIAPSTLDEIASAGKPMEKLPILAPVTGVVVEKNVVQGSAFTAGQTLYKIAPIHPVW
                     VIANVFQYELPLVKTGMTARILPPFPGEPAREGRVSYVNPFLDPDTRTGEVRAVAPNP
                     RGDLKPGMFVDVLLERDLGRKLAIPESAILYSGDRRIVFVDLGQGRLAPREVTLGAKA
                     GDRYEVVGGLREGDVVVTSGNFLVAAEARLRSATTKW"
     gene            complement(27250..>28341)
                     /locus_tag="VFR25_07555"
     CDS             complement(27250..>28341)
                     /locus_tag="VFR25_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9940942.1"
                     /translation="DATGRFMSPVLDGMVAPQAIGNEDVAAPGYQIGLSQSLPLWGTR
                     GLEKKAARAEARAAGEDQRAARLDLLRETRRLYYEFYLVERGLEVNQEQKDLLGHIRS
                     LAVQKYASGVVGQQDALQAEVELAMLDHEAIVLARDRRIVRARLNAVLHRAPEEYLPA
                     PLDSIPAPQEGDEPFAGPSASSSRPDVKRAEAERDARAAELELAGKGRIPELTLVARY
                     DAMWAEHEMRPTVGAEMSLPFLFGRVSGSEREARAGLARREQERLAVIDRARLEIEEA
                     LARVQETRHEIHVVESSVLPATERALASIRSAYESNRSDFAALLNAERDLARTRLDGH
                     RARVAYRLALADYERAIASDAGPAVEVPR"
BASE COUNT         4581 a         9603 c         9570 g         4587 t
ORIGIN      
        1 cttgctcagc cgctcgtggc tctgcggatc gatagccgtg acggtccact cgtacgttcg
       61 gctcggtccg aggtagccgt tgtacacgag gaaggtgtcc ggagtcgtcg tgctaaagat
      121 gcaacaaggc tgcggagcga ccacccggag gtccagccga tagtggacag cgcctgcctg
      181 gggatcgatc gatttgttcc accggaacgt gaccggcacc tgcgtcaccg agccattgtt
      241 gatgggcacg cgtaggttga acgggttagg agcgaccggc accccgtagg acgacccgta
      301 gatcgccttc acgacgttga tccgtccgtg gccgaagtcg ttgtcgggtg tagctgcctt
      361 gtcggcagtc tgcatgagcg cgtcccgcac ctgcgcgacc gtccactcgg ggtgcgcctc
      421 gcgcacgagc gccgcggctc caccgatgag cggtgtggat agcgacgtgc cgctcgcaag
      481 cgcgtaggtc gtggcgccgg ccaccgccca ctggacgttg atcccctggg ccacgacttc
      541 gggcttcatc cgtccgtcag cggtcggtcc acggcccgaa aacgcggcga tgaagttcat
      601 atcgctgaca gccccgaccg agaggatgct atccgcgtcg gctggagacc agagcgagcc
      661 ggccgcacct ccttcattgc ccatggcgtt gcagaccacg atccctcgac gcgccgcgaa
      721 gatcgccccc agagccacga ccgtggtgta cccgtcgagg tccgtgtact ggtagtcgtt
      781 cgcggtcccg tcgaaatcga gatacgcgag cgaagacgag atcacgtcga cgccgatgga
      841 atccgcccac tcagctgcgg cgacccagtt gtcctcttcc acgggcgtct cggagcgtac
      901 atcttcggtc ttggcgagga cgaatcgcgc attgaatgca ggaccgacga ggttacttga
      961 cgcgtagccg ccgagaacgg accaagtccc ggttccgtga tcccactgcc cggtcacgtc
     1021 ccccgcctgg ttcgccgtct caccgtcgtg gaagatgaag tcccgctcgg cgatcttttg
     1081 gagcagtgtg agtgacggat gggccttgtc gtacccggtg tcgaacatcg cgacgatcac
     1141 cgaggctccg ctccatccgg agtcatgcgc cgcaggcacg ttgatgccgt tgagcgcggt
     1201 gatcgaccca ccgtagctga agggcttcgg gagcgccgtg aactgccccg ccacggtccc
     1261 cgccagtaga tccccgggcc tcggtcctgc gtcgcgcagg aatccctgct ccgacggtgc
     1321 ctgcggcgcg ccgggcgcga cgcccaccgc ttcgattcgc cgcgagactc tcgccggcgt
     1381 gacccgtcgc acgaacggga gcgcggcgat ccttcgcgcc tgcccttcgt tcgcaatcac
     1441 ggtcatggcg ttgagccagc gtgagacgtg ccgcacatcc acccccgtcg cggcgacagc
     1501 atcgacgtag ttctgtgcga ccgggagatc gtagtaatcg ggaacgaagc ggccgcccgt
     1561 ctcccgcgca cggcgtgcgc gcgcacgcgg atccatccgg tcgtccacgg aacggagcga
     1621 ggcggcgaag gagccggcgt ccggaatcgc cttgtcggtg aaatgcaccc agaggaccga
     1681 ccgcccttcc gccttgagcc gcgcgagcga ctgcgcggac agcacgtcgg gggcgcgcgg
     1741 cgagggagcg gtgaacgccg cgtgcggctc cgggcgcgtg cgagcggccg cggaaggaac
     1801 ggcggggccg gctgcgatga acgtgaggag cgcgcccccg atgagatacg acctagcgat
     1861 gcgtgacatg acgggacctg ttccggctct cgaaggctgg accttcaacc gttgcgcggc
     1921 catcctaggc cgacgccatc gcctccttgt cggcgcgggc ctgggccacg atgcggtcgg
     1981 caagctctcg gggaacctct tcgtagtggg cgaacttcgt gtgatgagac gcgcgccctt
     2041 gggtgaggga gcgcaggtgg gtggaatact tgtagagctc ggcctggggc acgtgcgcgc
     2101 ggatgacgtc gtagcggccc tcgctcccca tgccgaggat cttcccgcgt ttggatgaga
     2161 gatcgcccgt gacggctccg acgaagtcct tcgggacctt cacctcgacc tcgacgatcg
     2221 gctcgaggag gacggggccc gcctccttgg cgccttcctt gaaggccatc gatcccgcga
     2281 tcttgaaggc catttccgag gagtcgaccg agtggtacga gccgtcgtag agcgccgcgc
     2341 ggaccccgac gaccggatag cccgcgagca cgccttcatg catggcttcg acgatcccct
     2401 tctcgacggc gggaatgaag ttccgaggca cggcgccgcc gacgacctcg tcgacgaatt
     2461 cgaacccctc gccgttcttc atcggctcga gccgcacgtg gacgtcgccg aactggccgc
     2521 ggccgccggt ctgcttcttg tgccgcccct gcttgctgac cgacttccgg atggtctcgc
     2581 gatacggaat gcgcggcttg gcggtctcga cgtggacgcc gaaacggcgg ttcagcttct
     2641 cgatcacgac ttcggtctgg aggtctccca tcgaggagag gatggtctgg tggaggtcgc
     2701 tcgccacgcg cacgtggatc gtcggatcct cctcgcggag cttgtggagg ccggagccca
     2761 tcttctcttc ctcgcccttc gacttcggag cgatcgcgac ttcgtgctcc gagctcggga
     2821 acgggatggg cgcgagcgtc acggggtggc tcttctcgca gagagtctgg cccgtgtggg
     2881 tctcgcggag cttcacggcc gcgccgatgt ctcccgccgc gatgtgtccc atgtcggtgc
     2941 ggtctttgcc cttgatcgtg aagacctggc cgatcttctc cgcccgctgc gcgctcgagt
     3001 tccacacttc cttgcccggc gtgaaattgc cggagaagac gcggatcatg gagaggtcgc
     3061 ctgcgtgcga ctcgctgctc gtcttgaaga cgagtgcgga cagatgcgcg gcgagctcgg
     3121 gcttcagatc ctcttcctcg gaaccgcccg cctttttcgc gtggaccgcg cccatatcga
     3181 cgggcgatgg ggcgagatcg acgagcgtgt cgaggaggaa atcgatcccg agcatcggga
     3241 ccgcggcgac gggaacggcc ggcacgaagg agcgatgggc cacgccttcg cgaaggccgg
     3301 tgtggatctc ctccaccgag agctcttctc cgccgaggaa tttctcgagg agctgatcgt
     3361 ccgcttcggc cgcggcatcc acgagggccg agcgcgcctt cttgacgtcg gcttcgagct
     3421 cggcgggaat cggcgcctcc ttgtgggagc cgtccttctc gaatatcagt gctttcatcg
     3481 acgcgaggtc gacgacaccg cggaactgat ccgcctcgcc gatgggccac gcgaccggta
     3541 cgaagcgaat cccgagccgc tcgctcgcag agctgacgac cttggagaac gaggcgtgct
     3601 ccttctccat catcgtcacc gcgcaaagga ccggacagtt gcgctcgtgc acccgctccc
     3661 agatcgcctc cgtgctcgcc tcgacgcccg cagcgccgcg cagacagaag agcgcggcat
     3721 ccgccgcgca caggcccgcg acgcaatctc cgatgaagtc ggcgtagccc ggcgtgtcga
     3781 tcaggttgat cttggtcccc cgccattcga cgggagccag ggccaggctg atcgtgatct
     3841 tgcggtggat ctcctccggc gtgaagtcga gggcggtcgt gccttcttcg atcttgccgt
     3901 gccgcgtgat ggccttggtt cggaagagga gcgattccgc gagagaggtc ttgccggttc
     3961 cgtgctggga tacgagggcg atattgcgga tctggctggg ctggaagtcc ttcaaggacg
     4021 cctccagatt cgaggaggta acttgcacga ttctatggac ataggcgatt acctatatgg
     4081 taccacagcg ggcgctctcc ttgcaaggag acgggctcaa agtaaaacac gagcggtggc
     4141 tcgcgccacc gctcgtgcgg gggttctgaa tgatgcttgc tgctagtgct gcttacttct
     4201 tgcgacgcgc cgcggcgagg ccgatgaggc ccatgccgaa gagcgcgatc gtgccaggct
     4261 caggcgccgt cgacgtggtc gacgtcgtcg aagacgagct ggtcgtcgtg ctggacgacg
     4321 tggtgctgct gctcgacgtc gttccgaaga acgcacggat ttcctcgaag ccgtcagcgg
     4381 agtcgagacc atccgtgttg cccgagagac cggagttcac gacgaagtac aggttgtccg
     4441 acgccgccgc acccgcgaag aagctcgtcg ggatgtagaa gtcgacgtcg ctctcgccgg
     4501 agcccgtgtt cgagacccga taatccgtat agatcgtctg gtccgaggtt ccgtcgaggt
     4561 tgtaccgcag ggtgccattg gcgatcaccg caggctcatc cgccagagag ccaccggcac
     4621 tggacgtgta gatgcgcatc tcattgagcg tgaggaacgc agcgtcctgg ttcggctcgt
     4681 tgatgtcgag cgtgaagacg tagtagtcga tcccgctcac gttcttgata ccgagctggc
     4741 cgagcgtgat cgagtgcgtc caaatacccg ccttcgtgtc gtacggcggc gggacatcgg
     4801 tgttcatgcc cgtctccgtg ttgttcgcct gccagcggag gaacggctcg atcacgcccg
     4861 ttccagagga ctggatcagg ttcgtgttcg tccagatcgt tccatccacc gcactagtca
     4921 cggtcgtacc agccgtcgtg agatcgatcg gcgggaacgc cgcgtttgcc gtagaagcgc
     4981 caagaagcgc cacgacggca gcaagtagca ctgccccacg aatcattcta gaccccccat
     5041 ttcgatttgc ctccccgcgg ggaacagcga ggccaccgga cttacttgcg cgaggaacga
     5101 cccccgtaga gcctcgcaca agctattcgt ttcgtaacct gaggattgta gcaaaacgcc
     5161 ctgaccattg caaatagaaa tttcaggcta gctgcctgtc ttcgcggctg ttccccgggc
     5221 cggtatcctg gccagctgct tcttgacgtc cgggacgaac gggctcctgg ggtaattcac
     5281 cagcaggttt tgaaggtaag cccgagcccg gtcgtcctgt ttggcctgat gcgcgacctg
     5341 ggctccctgc agaagtgctt gctccataat aggatgcccc atgtccctcc gcaccatctc
     5401 atcgatctgt cggaggactc cctgctggtc tccgaggagg gcatagcacc gggcgatgct
     5461 ccaacggtag aaagtcgtgt accccgaggt cgtatcccgc tcgatcagcc cctgataggt
     5521 caggatggcc tttcggaccg cctgcttcgc cgcatccgct tgtccgatac ggacataccg
     5581 gtttgcgatg gcgatcggcg cctcgagggc cgccctggtc tcggggtacc gaaccggaat
     5641 cccgtcgagc aggttcttgg cctgctccca gttgccgagc ttgtcctcaa gcatggccct
     5701 gcggaagaag gccggcggtg cgatgtccgg ttggttgccg taggaggtgg tgacctgccg
     5761 gtagtagttg agggcctctg ggaaccgact gtggagctcc agcagggtcg ccgcgtccat
     5821 cagggcgcgg ccggcgaagg gagacttggg aaagtccttc gccaccttct cgaacagggc
     5881 caccgactcg ttcgggttct ccttgcccgt gtgcatcatg tgcagcttgg cctcggagta
     5941 gcggatctcg ggctcgaggt ccttgagcga gggcgaagcg gcggcgaggc ggcgaaggat
     6001 cgcgacgtcg ttcaacccct cgctccaatc cccttgctcc aactccaccc ggatgagacg
     6061 cgcgcgcacc tgcgcgtcga gaatcggcga atgcggggtc gcaagaacgc ggcggtagta
     6121 cgcgcccgca tcgttcagag ccgcgttggc ccgagtgcta tcgttcaggt cgcgatagag
     6181 gcgaacgatc gactccggga tggcaaggac tgcgtcctca tcctgcgtgc cttgcggcga
     6241 gatcggcggg taccgctcga gcatcgtctt catgagagcg accgcttcct cgtagcgctt
     6301 gtactgccgg agcgtaccga ccaggaagaa gtccgcccgg cgcagcatga gggtgtcgtt
     6361 ccccgccatg gcccggactc gcttcatgct ctcgatgccg aggtcgggcc ggttcgcttg
     6421 cacggacagc cgcgccgctt ggaggtcggc gttgccgacg aggcgcatca cgtcgatgga
     6481 gagcgtgctc tcctcctccg acctggctgc cgatagcgga ggaatcttca ccttcttcgc
     6541 gacgtcgaga tagggcgcgc gcaagccaag gagcgtggtc gagtcgggat tttcgtccct
     6601 gagccgcacc gcggactctt ggaggtcggc gtgccacagg agctgctcgg cccggtagcg
     6661 ggtcgcgcgg agggacgtat caccgcaccc cgaaaggacc aaagccagcg cgaggagcgc
     6721 gagccacgaa cgcgcaacgc gggtcgtccc gccgagccgg ttcacgcttc ctccacctgc
     6781 gaggcccaat ggagccgggc cgcagcagca taggtcaggt acgtggccac gctgatcagg
     6841 atcgcgtaga tggcaagcac caccgcgatc atctcgggcc gcatccgcgt ggccaagaac
     6901 gcggtcccga gctggaccgc catgaggaac ggcagcgcgg gaagcgcgaa gacgaagagg
     6961 tagagcaccg tccacttgaa gagcctgacc ccttcgcgga tgccctcacg cagcgccttg
     7021 acgagcgatg agcccggctg gagagccagg atgggaacgt agataaagag cgcgctcaac
     7081 gaataggaga cgacgaggct caggccccag atcgccgcgg tggcctgcag ccctggaaac
     7141 gaagcgcgga tgagcggcac gagcgccacc ttgaagagcc attgccatcc tgaaaggagc
     7201 gcgaaagtga ggagtcccgc gatcagggtg ggcggccacg cgcgattcac ccttgccttg
     7261 aatcccgggc cctggagatc ggggtccatc ggcgtctgga cgcgcgcgat cgagagcgga
     7321 atcagatacg cgcccgcgac ggtgtacagg atcagctccc accaggaggc ggcgttcggc
     7381 agatagagga agaacgtcgg atagtggagc ggcgcgtctc cggcgaagag cttcacggcg
     7441 gcgccggccg tgcccatgct gatcgtggtt ccgagattca aagcgaggag gaggaagagg
     7501 agctgggcgc ccaggatcca agcgaagggc gcgtattcat gccactggaa atcgcgcgcg
     7561 gcttccacgc cgcacgcgcg cgcgacccgg aagtctcgac gcatagtcct ccttggctta
     7621 cgggttgcaa ctcggagccg gacgccggag actagcgcgc catggccacc gatgactacc
     7681 agattcagat ccggcgccct ggcgccgagc ccgaggtctt cttgctggac aagaacgccc
     7741 cctgcacgct cgggcgctcc ccccagtgcc gcgtcttcct gagcgacgct tccatctcgc
     7801 ggaaccacgc cgagatcttc tggcgcgacg gatccttctg gatccaggat ctcggcagca
     7861 agaacgggac gaagctcggc tccaagaaga tcgaggggcc gcatccgctc aagccgggcg
     7921 accagctcca tctcgggagc gtccacctct cgttcaacaa ggcaggcgag gcgccatcgc
     7981 acgagacgag cgtctcggcc cgcgtcgcgg acatcgccgc tccggtcaac gtgcacgccg
     8041 tgccgctcga ggatatctcg agcgggagcg tcgcgctcga cacgcgctcg ttcggctcca
     8101 gcctctcgcc ggagcgcgtg ggcaacttcc tccaggcgat ggaccgggtc ggccaggcgc
     8161 tcctcgcgta ccgcccgctc gacgagctct tccaattcgt ggtcggcctc gcggcggacg
     8221 tgctccgcgc ggaccgcacg gccatcctcc tccgccgcgc gagcgacgac gacctggtca
     8281 cgatggctgt gtcgcagagc ggccgcggcg ccggggagga aatcatcgtg agccggagca
     8341 tcgcgcgcat ggcggtgggc cagcgccagg cgattctcac gagcgacgcg cagagcgaca
     8401 cccggttcaa ggagcagcag agcgtgatcc gccagcggat ccactccgcc atgtgcgccc
     8461 ccttgtggca cgacggcgag gtgctggggc tcctctacgt cgacaacgtc gcggcgccgg
     8521 tgcccttcga ggagggggat ctaaggctcc tcacgttcat cgcgcatctg gcggcggtga
     8581 agatccgcga gaccgagtcg cacgagaagg atctcgagcg ggaggaggag ctgcgtcgcg
     8641 ccgccgcgct ccagcaggcg ctcctccccc acgagccccg cgtggccggc gtgatcgagg
     8701 tcgccggccg gaacgtcccc tcgctgaacg tcggcggcga ctacttcgac ttcgtggacg
     8761 ggggtgagaa tcggatggtg gtggggctcg gcgatgtcgc gggcaagggg atgccggcgg
     8821 cgctcctcat gacccacctg gccgcgagcg tgcgcgcgca ggtcgagacc gagcgcccgc
     8881 tcctcgaggt gatgtcgcgg ctcaaccgat ccatctacca gaacgtgcgg ggagagcgct
     8941 tcatcacgct cgttctcgcg gagatcgaca ccacggacgg atcgatcctc tacgtgaatg
     9001 gcggccacaa cccgccgtta ctcctgcgtt cgggaacgaa cgaaatcgag atgctctcgg
     9061 aaggcggcct gctcctcggc atcatgccgg aggcgaagta cgcgcaagga accgtgacga
     9121 tgggtcccgg cgacatcctc ctcttctaca gcgacggcgt gacggaggcg aggaacctgg
     9181 cggaggagga attcggcgag gaccggctga tgaccttcat cagggaagcg cgcgcgctca
     9241 cgccggcgca gatggtggag accttgatcg agagggttcg agacttcagc cgccgcccca
     9301 agccgaccga cgacgtcacg gtcgtcgccg tgcgccgcgc ctagccccga ctttcgctcc
     9361 ggcgggtcag gcggagcgcc gagcggtcct tacccctccg ttgctcctaa gctccgcgtt
     9421 ccgctgggcg gcgatcgcct cgtccaggct ccgctccacg agcttggcgt ggaggatcga
     9481 gtcgcggtag tcgcgatggt gaaacgcgct cttcgccttg gcgagctgtc cctgcgcttc
     9541 gcgccgccac ttgagcgcac tctccttggg attcgcaccc agctcccggg tcgcgcgatc
     9601 atacgcatcc tgcgcctgcc tgagcgcgcg ctcggcggtc ggcgccggca cgggaaggtt
     9661 ctgactgtcc tgtaggagat ccagcacccc gtcccggacc tgcatggccc ggcgataggc
     9721 aacgcgcgcg tcccccgatt cgagcagctg ccgcgattct tggagactgt cgcgcagcgc
     9781 gtgatagcgg cgccacacct cggggttcgc tccctgatcg aagacatcac gggcgagtga
     9841 gagcgcgtcc tcggcgttgt cgatcgctcg cccgacgcag acgggatcgt cctccggcgg
     9901 cccgaccatc accgcggctt cgcgcgcgag cgagcgtgcc tcgcgcgtca agcgcgtggc
     9961 gcgggcatag agattccggc tgaacgcgtc gcgcgcttcc ttctcgcgcg tcacggcctc
    10021 gccgaatcgg ctctgcgcct gggtgtttcc ggtgcggtag accttgggct tgaggacggc
    10081 gaggacccga tcggtgtggg tcagctcccg ctgaatgcct tccgattcgc tgaggggggc
    10141 ctcacccgtg tccgaggaga tcgacgaaga agggccggcg gcgttcgaaa cagcagcagt
    10201 gccggtggcc ggaggtgccg ctcggcccgc cctcgtctct aagcgctggc ccgagacccc
    10261 aagagaagac acggagccca gggtcatggc ggtagccagc gctaacgtga tccgcacacg
    10321 ttccatgttg gtcggtccgg tcggcgttac ggatcacggg ggggtactga tatcggcgtg
    10381 tgcatacctt gggtaccgca agctacatgc cgcattcgag gcttggatcg aggcgggatc
    10441 gggcggaccg caaacgatga gggatcacgt cgttgccgcg cgtcgccgac gcaggctttc
    10501 agtcccggcc aaaaaagagc cgtgccacgc tgcacgcgcg cgtaccgcgc gtgtacggaa
    10561 ccgtatgggt ccgggtaaac gcgcccggtc tagaacgcgg gcgcttcgct cccctcgggc
    10621 tcgggaccgc cgatggggat cccgtggctc tccatccggg aatagagggc gcggcgcgag
    10681 atgccgagga gggtggccgc cttcgtcttg tttccctgcg cacggtcgag ggcttccttg
    10741 atgagccgct tctcgatctg ctccaggctc agccccaggg agagaagatc ggcgggatcc
    10801 gcggcctgac gcgcgcgacc gagcgggaag tgatgccgct cgatccgccc gccccggccc
    10861 gcgagaatgc aggcccgctc gatcaggttc tggagctcgc ggatgttgcc cggaaagtcg
    10921 tagttgcgca gatactcgag cgcgtcgggc gtgagctcgt cgatggtgcg ctcctgcatg
    10981 atgaggaagt gccccacgag cgacgggatg tcttcgcgac gctcgcgcag cgcgaccaga
    11041 cggatcggga agacgttgag ccggtagtag aggtcctccc ggaagcgccc gtggtggatc
    11101 atctcctcga gatcgcggtt cgtggccgcg aggatccgca cgtcggcccg gcgcatgtcc
    11161 tcgcctccga cccggacgta ggtcctgtct tgaagcacgc ggagcagctt cacctggatg
    11221 ctctgcggca gctcgccgat ctcgtcgagg aagaccgttc cgccctcggc gacctcgaag
    11281 cgacccggct tcgtacggat ggcgccggtg aacgcccctt tctcgtggcc gaagagctcc
    11341 gactcgagga gcgtctcggg gagcgcgccg caattcacgg gaacgaacgg cgcgtcggag
    11401 cggtggctcg cggcgtgaat cgcctgcgcg aagacttcct tcccggtgcc gctctccccc
    11461 gtcaggagca cgttggcgtc ggtgggcgcg acgcgctcgg ccaggctccg cgcatcgcgc
    11521 atgggaaggc tcgttccgac gatcgaatcg aagcggtgga tcggaatctg ctcgcgcagg
    11581 ctctcgatcc gcgtcttcaa gaccgtggat tcctggcgca gcttccggtg atccaggacc
    11641 tgacgtaccc ggaccgggag ctcctccaga aggttgtcct tcatgatgta gtcggtcgcc
    11701 ccctccttca tcatctgcac ggcctcgcgc gtgtcggcga aggcggtcat caggatgacg
    11761 tccggcagat atccctgctg gcgggcctcg cgaaacacct ggacgccgct cgcgccgggc
    11821 atcctcatgt cggagatgac gagatcgaac gtgtgctcct ggatctcgtg gatcgccgcg
    11881 tcccccccga gagcgatcga gacgtcgtac ccctgctcgg acagagtcgt ctcgatcagg
    11941 cggcagaggt tttccttgtc ttcagcgacc aggatgcgtt cgggcatgct ccataccttc
    12001 cggtggcttg gggattcctc gattgtaggg ctcagggacc ttcgaggggg agccggactt
    12061 cgaacatggc gccgcccttc ggggcatccc caactgtaac cgatccgccg tgctccgata
    12121 cgatccgatc gacgaaggcg agtccgagcc ccgtcccctt ggcccgggtg gtgaagaagg
    12181 cgtcgaagat cttcttgcgc aggtctttcg gcacgccggg gccggtgtcc tcgaactgga
    12241 tcctcagctc cccccgcttc tcgctcaccc ggatcacgag tttccctccc gagggcatca
    12301 catcccgggc gttgagcgcg aggttgagga agacgtgatg catcgcgagc ggatccccga
    12361 tcaggacgac cgacccgccc gcgtgccatt cgagcaccgt ggagacgctg gcccggtcga
    12421 gctccacgcg aagcatctcg agcgatcgct cgagcacggg gcgaagctcg aattcctgcg
    12481 gctccagatt gggccggcgc gacagatgga ggaagttgtt cacgaacgcg ctcatccggt
    12541 tcacttcctc gtcgatcatg ctgaggagct ccgcctgctc tttgggctgg gtctcgcgcc
    12601 gcttgagcct cgacgaggca ccctggagca cggccagggg attccggatc tcgtgcgcca
    12661 cccccgccga cacctgcccg atcgacgcca gaatgtccgt gtgccggaga ttctcctcga
    12721 tccgtgcgag atgtccgacc gtgcgcacga agccgaggcc gatcaggatc acgagcacgg
    12781 atgagagcac gaccacgccc gccaccgacg cccggatccg ctggagcggc tggaacgctt
    12841 cggatcctcc ctcgacggcc acggctccca ccaccccacc ttccggagtc aggacggggg
    12901 cgtagcccga gcgcagatac tggccgtcct tgtagtagag ccgcgagtgg gaggcctgcc
    12961 cgatcaacgc cttggtcatg gggaccgggt cgagcgtgac cacgtccggc tcgtacgtga
    13021 acggatcacg aagccggagg agcacgcgcc gatggcggtc cacgagcacc acgttcgaga
    13081 ggcccgcgct ctgcgcgagc cgcgtccact cggcgcgcac cgcgtcggcc gcttcctggg
    13141 aatccatctt catcgagaaa tcggtgaggg cggtgtcggc cgcggcggag gtgtcggcgc
    13201 ccgccaggga aagcatctcg aactgctccg gcgccacgag gagcgccgcg atgtgggcgg
    13261 ttccctggag ccgcttcccc agctcctgat cgaggagctt ctgcgacgtc ttcaaggaaa
    13321 agaagatcgc gccgttcacg agcacgagga gcgcgatgag gaacgcgagc gtccacacga
    13381 tgcgcggacg gatgttctgg cggcggggaa acattcggag tgggcggggc tagggcagcg
    13441 gaggatccgc accctggccc gggtagggcg agcgtaccga ccggcgcttc cccatgtcaa
    13501 cgcgcgggac ctgtgctagc gtgcgccgac catgaatcgc acgctcctcc tcttcgcctt
    13561 cggcctcctc gccggcgcgc tcggcggcct catgggtgtt gggggagggc tcgtcctcgt
    13621 gccgctcctc gtccgcttcc tcggaatggg ccagcacgag gcgcagggaa cgtccctcgc
    13681 cttcatcgtg tcggccgcgc tcgtcgcatc ggccaagtac tacggaagcg accggctcga
    13741 tgtgcccctg gccctgacga tcgccgcggg cgcggtgccg ggcgtcctcc tcggatcggc
    13801 tctcgcggcc cgcacgtcgg gacggaccct caggctctgc ttcggcgtcc tgatcgcgat
    13861 caccgcgatc cgcatcctcg ccttccctcc cgtccacgag cgcgcgggct cgctctggcc
    13921 cgccgcggcg aacgtcgcac tcggcctcgg catggggacc ctcgcgggac tcctcggggt
    13981 gggcgggggc atcctggtcg tgccgtcgct cgtcctcggc gaaggggtcg ggcagcacga
    14041 ggcgcagggg gtctcgctcc tggtcatcgt gccggtggcc atcgccgggg tgatcagcta
    14101 tgccgcccgc ggccggatcg cccggggctc gatcccggcc accatggcgg ggggtgcggc
    14161 cggcggcttc ctgggcgcgc tcgccgccca ccacatccgg ggggagaccc tgtcgcgcat
    14221 cttcggcgcc ttcctcctgg tcgtcgccgc ccaaatgatt ttccggcgcg cgcgtggtac
    14281 ggtcccgcga tccctgggag ccgagtccgg aggaattcca tgaccacgaa gacccgcttt
    14341 gcgctgttcc ccatcctcgc cctgaccttg tccctcgcgt gcgcttccca gccgagtcct
    14401 caagagaagg ctgcgttcga gacgctcgcc cgccggtacc tgatggccgg ctacgcgttc
    14461 aatccttcgt tcgcgacagc gtggggcatc cacgactacg acgactcgct cgagaattgg
    14521 accccggagc ggatccactc ggaagagcag cgggtgcggg acgcgatgaa ggagctcgac
    14581 gcgatccgtc cggagaagct cgatccggcc actcggatcg actacgacct cctccgcacg
    14641 ggcatgaacg gaaacctcaa ctacctgacc gagctccgag gctgggagaa cgaccccggc
    14701 cagtacaact acggcggcac gctcttgagc atgatgaccg tggactacga gccgctcccg
    14761 gagcgcatgc gctcgctcac gcggcggctc gagcaggtgc cgcgccagct cgccaacgcg
    14821 cgcctcaacc tcaagaatcc accacagatg ttcacgcagt tcgcgatggg ggacttcgag
    14881 ggcacgatct cgttcctcga gaacgacgtc acgcgcgcgt tcgcgtcggt gaccgatccc
    14941 acgcttcgcg ccgagttcga cgcggccaag aagaccgcga tcgcggccac gaaggatcac
    15001 atcgcctgga tgaagtcgat gctcctcccg gcgagccacg gctcctacgt cctcggtgag
    15061 gacaagtacc ggaagcggct ccgctacgag gagatgatcg acctcccgat cgacacgctc
    15121 ctcgcggtgg gccagcgcga gctggaccgg ctcgaagcac gctacaagga ggccctgcag
    15181 cgggtcgatc cgagcgcgac gcaggaggcg atcgtcgccc gcatgcgggc ggaccatcca
    15241 cccgcggaca gcatcctcga ttacgcgcgc gcgctgtgcg aggaggcgcg ggcgttcacg
    15301 atccagtcgg gcttcatcaa gatcccggac aacgcgcaca tcaccgtgcg tccgacgccc
    15361 gagttcgccg cgagccgctc gttcgcgagc ttcgacgcgc cggggccctt cgagaccaag
    15421 gcggacgacg cttactacaa catcacgctc cccgatccca agtggccgaa gcagcagatc
    15481 gaggagcacc tccagggatt caatcgctgg acgctcccct ccgtctcggt gcacgaggtc
    15541 taccccggac actacgtcca tcatctctac ggcaagcggg cgccctccat ggtgcggaag
    15601 tcgatcggat cgggagcgtt cgcggaaggc tggggcctct ataccgagga aggtctcctc
    15661 gacgagggct tccgcaagga cgatccgcgc gtcgaggtcg gcgtcatgcg gtgggcgctc
    15721 gtgcgcgcct gcaggctcca ggtcgggatc cgcgtccaca cgcgcggcat gagcctcgag
    15781 gacggcacga aattcttcat ggagcacgcg ggcatggagc gcgcgaacgc cgagcgtgaa
    15841 tcggggcgcg ccgccttcga cccgacgtac agcgtctaca cgctgggcgc gctcgagatc
    15901 cggaagctgc gggacgacta caagcgcgag cagggcgcca acttcaatct cggccagttc
    15961 cacgagacca tcctctcgca gggggcgctc ccggtggcgc tgcttcggaa aatgctgctg
    16021 aagcacgagg gtacgatcct ctgacgaagg gacgtcgccg gctcgtcctg gctctcgtca
    16081 gcgcctcgcc cgcgtggagc attccttcgc gcgaggtgga ggcgcttcgg cgggacttcc
    16141 ccgacgtcga cgtgatcgac acgcgcacgc gggaggagct ggacgccgcg gttcccgaag
    16201 cggacatcct attctcctgg gcgttcgggg acgaggtgat cgcgcgcgcg aagaggctcc
    16261 ggtggctcca cgtgccggcc gcgggcgtgg gctcctatct cacgccgagc tttcgcgcga
    16321 gcggcgccgt catcacgaac tcgcgcggcg cgcacgccat tccgatcgcg gagcacgtga
    16381 tggccgggct cctcggaatc gcgcgccgga tgaaagccgg gatcgtcgag cagacgacga
    16441 ccggcatgat ccgcgagaac tggtgggccg aggaccaggt tccggacgag ctccacggca
    16501 agacgatcgg cctcttcggc tacggcgcga tcggacgcga ggtggcgcgc cgcgcggtgt
    16561 cgttcggcat gcgcgtgatc gcgctacgac gccgcccgca cggcgctccc gcctgggatc
    16621 cggagctact tcaggcgctg gacctccctg ccgaagaacc acctgtggcc gagacgttcg
    16681 gccccggcga tctcgaccgg tttctcggaa ggagcgacgt ggtggtggtc tcggcagcgc
    16741 tcacccaaga gaccgaagga gcgctcggcc gcgcccagtt cgcacggatg aaaccgggag
    16801 cgtggctcgt gaacatcgcg cggggcaaga tcgtccagga agcggagctc gtcacggcgg
    16861 tgcgcgaggg acggattcgc gcggccgcgc tcgacgtgta cgaaagggag cccttgccgc
    16921 gcgagagccc gctctatacg ctcgatcgcg tgatcttgac gccgcacgtc tccggcctgt
    16981 cgcgcggatt ctggccgcgc gcgatggccc tcttccgcgc caacttgagg cgcgatgctc
    17041 gaggcgagcc gctcctgaac cgcgtcgatc ccgacctcgg ctactgagga acgcgatgcc
    17101 ccgtccgctc ataggaatcg gccccaacta ccggctgaag cagcgccgcg gggattccta
    17161 cgtcctcgac gcgaactaca tgaagctgat cggcggcgcc ggagggcttc cgctgatcct
    17221 gccgctcgtg tcgacgctcg aggaggcgcg cgaggtggtg ggacgcgtgg atggactcgt
    17281 gctctcgggc ggctccgacc tcgagtcgga gcggtacggc caaggcacgc ggcaccccga
    17341 ccggctcggc gcgccggccc ggaacgcgtc cgatctcttc ctcgcgcgcg ctgcccagga
    17401 gcgcgggatc cccacgctcg ggatctgcct cggcatccag gtgatgaacg ttgaattcgg
    17461 cgggagcctg ctccagcacc tggacgagga tcgtcctggg ggaatgaagc acgaggaaga
    17521 aacggacgac gagacgccgg agcacacggt gcacgtcgag cccgggacca cgctcgccaa
    17581 ggtcctcgga caggagcggg tggtcgtgaa ctcctaccat caccagtccg tggaccggat
    17641 cgctccgggg ttccggatcg cggccacgag cgaggacggc gtggtcgaag cgatcgagcg
    17701 ccccgaccat ccgttctacg tgggcgtgca gtggcacccg gagcggatgc ccgacgccct
    17761 cgccacacgt cgtctcgtcg gcgcgttcgt cgacgccgcg cggggcgcct ccatccgctc
    17821 ctagcgcgcc gcccgctcta cgccgccgtc tcggcgagac ctaccagccg tacttctttt
    17881 tgatcgtctg gatctggtcc aggtggttga ggtcgtgccc cgcgatcagc tcggctgtcc
    17941 gccgcacggt ctccttgccc cgctcggagt gcatgccgta gcgctcccac gtccggcgcg
    18001 gcatcgattc gatcaggtcc aggttgatct tccggagcgt ggcgatctgc gccatgagcc
    18061 gcgcgggatt ccgattccga tgcttgagcg cgaccgtcca cgtggcctgg tcgtagccgg
    18121 ggatgggcac cccgggctgg gagagcgcga gccggtagcg gtagccgtag acgacctccg
    18181 tgtcgacgag gtggcccagg atctccgcga tgctccactt gcccggcgcg ggacgcttct
    18241 tgagctgggc cgaccggagt ccctggatcg cgcgagcgat cttcgcgggg ccgacgcgca
    18301 tgagcgggag cggagcgcgt ccgccacgat aggcgtcgat cttccggacg taggcgcgga
    18361 tctcgggcgt gatgcgcacg cgcctggcgg cgcgcttggg tgcgcgattt ctggctcgac
    18421 ccgcacgaac gccgcgtgac cccttccgtg tgctcttctt cacgaggcat ctcctttccg
    18481 gtgggtgggg caagctggat tcgagcggcc gagggtagcg caaccggtgg cgttggaata
    18541 tgctcccggg gtcggctcgc ccggatccgg gctggacggg cctcaccggg aggtttccat
    18601 gcgccgaccg ccgttcccac tcgcccctgc acttctgctc gccgccttat tccttggagc
    18661 ctcgaccgca ctcgccgatt cggagaaagc ggaaacgttc cacatcatgg agatcgtcca
    18721 ggtgatggcc gcctacaacg gcaacacggc catccaggcg gtcgagatga aggagcttgc
    18781 cgccgggcaa gatctggtcg cgtccggcca gatcaacatc tacgacaagg atggggtctt
    18841 ggggacgacc ctcgggacgt tcgctgcgaa cgtggccaac ggggcgagcg gcgcccacat
    18901 cctgtgcgcc acggccgcgt tcgcctcggc gttcagcatc acaccggacc tcgtcctctc
    18961 gcccggcatc ccgctcccgt ccggccaggt ggtcttcgag aaggtggggt gcaaggtgaa
    19021 ctcggtcccg tatgggggca tcagcacgat cgtcgccaac acctccgtcg cgccgacgat
    19081 cccgacgctt ggcgccacgg cactcgtccg gaccggggac gcgcccacca ttacgacctg
    19141 ccccttgacc gacagcgccg cttcccggtt cgcgattcga tcggggacct cggcgtcgtc
    19201 gttcctcgtc ttccagacca acgccggcga caccgcgcac gtctggtcca cggcgaccgg
    19261 cgtcgggacg acgccgccgg agccggtccg gctccaggcg tcgccgaatc cgttccgtgc
    19321 ggagacgcgg atcgacgcgc ccgactgggg accgctcgcg atccatgacg tgcaaggacg
    19381 actggtgcgg gtgctcacgt gcgcgccggg cggcgcttgt ccgcaggtgg ccggcgcgtt
    19441 tcgcggctcg tgggacggcc gggacgagcg tggacgcgcg atgccttcgg gggtctacta
    19501 cttgaggtac tcgggagcga gcggacacgc cgtcaaggcg atcgtctatc tgcgctgagg
    19561 cggggcgcct cgcttaccgc acgacgatcg tgcccttctc catgtccatg ccgcaggcga
    19621 acgcgacctc gcccggcttg agcgggccga gcgccacgcg caccgtctga ttgagcggga
    19681 gctcctgcgt gatcccgcgg gacgggacca ccatctcggt cgcgcaggtc acatcggtct
    19741 tgcgcgtcac gaggagcgtg acggcctcgc cgtgcttcac ctcgatgcgg ctcggctcga
    19801 agccgttctc ggtgaccgaa accgggatct cggccttccc gccgccgcat ccggcggccg
    19861 caagtaccag gatcgagata gcgatcgctc gtctcatgcc aaccctcctc gtcggtccgg
    19921 cccacgtcac aggcgcacgc cccggagccg gttcgagttc cagatcacgg acaccgagct
    19981 caggctcatg gcggccgcgg cgatgatcgg actcaggagg agcccgaaga cgggatacag
    20041 cacgccggcc gcgatcggca cgccgagcac attgtagacg aacgcgaaga agaggttctg
    20101 gcggatgacg cccatcgtcg cgcggctcag ccggcgcgcg cgcacgatgc cgcggagatc
    20161 gccgcggatc agggtgatgt ccgcgctctc catcgccaca tcggtgcccg tggccatcgc
    20221 gatgccgacc tgggcctgcg cgagcgcggg ggcgtcgttg atgccatccc ccgccatcgc
    20281 gacgaagcgc ccctcggcct ggtagcgctt cacgaactcg agcttctggt cgggcaacac
    20341 ctcggccacg acctcgtcga ttccgaggct gcgggccaca gcgttcgcgg tcgcggcgct
    20401 gtccccggtc agcatgacga tcttgatccc gtccgcatga agggcctgga tcgcctcctt
    20461 ggtggtcggc ttgatcggat cggccgcggc gagcagtccc gccagccgtc cctcgaccgc
    20521 gacggccacg accgtccggc cctcgacgcg gagcgcttcc gcctgctccc acagatcgtc
    20581 gccgcgaacg ccgagatcat ccatgtgctt ccggttcccg agcgcgacgc gcttcccctc
    20641 cacgatgccg gcgactccca tgcccgacat cgattcgaac tcacgcacgt cggcgatctg
    20701 caggcgccgc tccagggcgc cggcgaggat cgcgtgggcc aacgggtgct cgctcgcccg
    20761 ctccaggctg gcggcgaggc gcacgatctc gtcgtcgctc catccatccg tcgccaccac
    20821 cgaagcgagc cgcggctttc cctcggtgag cgtccccgtc ttgtcgacga ccagcgtgtc
    20881 cacgtcgcga agcgtctcga tcgcctgcgc gttccggaag aggacgcctg cggtcgcccc
    20941 ttggcccgcc gccaccatca ccgccatcgg cgtggcgagc ccgagcgcgc agggacaagc
    21001 gatgatcaag accgcgaccg cgttcacgat ggcgtgcgcg agccgaggct cgggtccggc
    21061 gagcatccat accaggaacg tgacgaccgc caccgccagg acgatgggca cgaagtagga
    21121 cgacacggtg tcggccaggc gctggatcgg ggcgcggctt cgctgcgcct cggagaccat
    21181 gcgaacgatg cgggcgagga gcgtctccga gccgacgcgc tcggcgcgca tgacgagtga
    21241 gccggtcccg ttcaccgtgg ctccgatcac gagactcccc gccgacttct ccacggggat
    21301 cggctcgccc gataccatcg attcgtcgac ggacgaggcg ccctcgagca cgacgccgtc
    21361 gaccgggatc gtctctcccg gacggacgcg gagactgtcg ccgacaagga ccgcctcgag
    21421 agggacgtcg tgctcgccgc catccggtcc gattcgccga gcggtcttgg gggcgagccg
    21481 gaggagctgg cggatcgcgg cgcccgtccg gctccgcgcc gagagctcga gcacctgacc
    21541 gagcagcacg agggtcgtga tcatgacggt gacttcgaag tagatcggca cgagtccgtg
    21601 ggcgtcgcgc agcgccgccg ggaagatccc cggcgcgaga accgccacga cgctgtaggc
    21661 gtagctcacg gagacgccga gcgcgatcag cgtgaacatg ttgagcgcgc ggtgtcgtat
    21721 cgaatcccat ccccgcgcga ggagcggcca cccgcaccac agaacgacgg gcgtcgcgaa
    21781 cgcgagctcg agccagtggc ggacgtgggc gaggccctcg agaggacgtg ccgggatcag
    21841 atccgcgatc gcgaacgcga tcaagggcac gctcaacgcc accgagatcc agaagcgacg
    21901 gcgcatgtcc gcgtactcgg gattttcctc gtcgtcgagg gataccgtca tcggctcgag
    21961 cgccatcccg cagatggggc acgagccggg ccggtctcgg atgacttcgg gatgcatcgg
    22021 gcaggtccac tgcgtgccgg ccgaggaggc gggcggggtc ggatcgtggg tcatcgcctc
    22081 gtgcttcgcc cccggcgcca agtagcgctc cgggtcggcc cggaacttgg cgaggcagcc
    22141 tggcgagcaa aagtagtagg tcgcgccctt gtagtcgtag cgacccgccg cgcgctcggg
    22201 cgcgacacgc atcccacaga ccggatcgat ctcggtcccg gctgtgggtg cgtcgttccg
    22261 gacctccagc ttcaaacccg tggtcttcac gtcactactc tacctgaatg gatcccgaga
    22321 ccatgtccat cgggcaggtg tagcggagct cgcccttcgt cttgggcgtg aacgtcacgc
    22381 tcacttcctg gtgcaggggg agctccttgc ggacgccggc cccttggatg accagctcgg
    22441 tggcgcacgt cgccgccgtc tcccgggtga cgaggagcgt gacgggcacc cctcgcttca
    22501 cggtgatctc cgccgggacg aagccgcggt cggacacggt catcctgatg gtttgcgagt
    22561 tcttctccga gcagccccaa acgcccccga gtagcgccaa ggcgacgatc ccgatgatac
    22621 gacctttcat gaccatcctc ctcaagcagg gcccggcgct cctgcgcgcg cgggcagatg
    22681 acgtcccttc caccacgcgt agatcgcggg gtagacgacg agctccagaa cgaacgaggt
    22741 gagaacgccg ccgatcatcg gagcggcgat gcgccgcatg atgtcggcgc cggagccgtg
    22801 tccccagagg atcgggagca gtccgaacag aatggcgcaa acggtcatca tcttcggccg
    22861 gatccgatgc gccgcccctt cgacgatggc atcctcgaga tcagcgcgat cgcggagcgc
    22921 accgcgctcg gcccggtccc ggtgcgccag gttgagatag aggagcatga cgacgccggt
    22981 ctcggcatcg agtcccgcga gcgcgatcat gccgacccag accgcgacgc tcatgttgta
    23041 gccgagcagc cacaggatcc acaccgcccc gaccagcgag aacggcacgg ccagcatcac
    23101 gatcatcgtc tcggtgacgg agcgaaggtt gaaatagagg aggccgaaga cgagaaggag
    23161 cgtcacgggg accacgagga ggagccgcgc gcgggcgcgc tcgtagtact ggaactgtcc
    23221 cgaccactcg aggcggtatc cgggcggcag cgaaacctta ccctcgaccg cgcgtcgcgc
    23281 gtcctccacg taggacgcca gcggccggtt ggccacgtcc accgagacga gcccgaagag
    23341 ctgtccgtcc tcctcgacca gcatcggcgg gccggtgcgg aactggatgc gcgcgacctg
    23401 ggcgagcggc acctggccgc cgctcggggt cggaatgagg acacgggcga gcgcatccgg
    23461 gtcgtcccgg aattcgcgcg cgtagcgaac gctgatcgga tagcgctccc gcccctcgac
    23521 cgactgggac accgtcatgc cgccgatcgc ggcctcgatc acgtcctgca cgtcgcccac
    23581 cctgagcccg tagcgcgcgg ccgcggcccg gtcgatgtcg aagtccagga aatacccgcc
    23641 ggtgacgcgc tcggcaaagg cggcgcgggt gcccttcact ccttggagcg cgtgctccac
    23701 ctcgatgccg atccgctcga tcgtggccag gtccgacccg aagaccttca cgccgaccgc
    23761 gctccggacc cccgtcgcga gcatctcgtt tcgggtctga atgggcatcc accagaggtt
    23821 gggcatgccg gggaaccggg agcgcgcgtc catctcggcc acgagcttgt cccaggtcat
    23881 tcccttcctc cattccgact ccggcttcag ggtgacgacg gtctccacca tggagagcgg
    23941 ggccggatcg gtggcggtgc gcgcgcgccc gaccttgccg aacacggtct ggacctcggg
    24001 gacctcacgg agccggcgat ccatcagctg gagcaccgtc gccgcctcgg tctccgaaat
    24061 cccaggcggc gcggtcggca tgtagaggat cgtcccctcg ttcaacgggg gcatgaactc
    24121 gctcccgagg cggaagaaga tcgggatcgt ggagaggacg aggagcaggg cggcgccgac
    24181 cacccagcct cgccgccgga ccgcgaagcg caccacggga ccgtaggcgc tcaccatgcg
    24241 tcgagacagc ggatgctcgc gctcggaacg gatccggccg cgaatgaacc atgcggcgag
    24301 cgcgggcgtg agcgtgaccg cgaggagcgc ggcgaagaac atggaatacg tcttggtgta
    24361 ggcgagcggc ttgaagaggc gaccttcggt cccctcgagg gtgaagatcg ggaggaagga
    24421 gatcgtgatc accatgagcg cgaagaagac ggggcgtccc acctctcgga gtcccgccag
    24481 gatcgcttcg tcgcgcgagc ccgctcgacc tcccgcctcc caggcttcga gcttcttgtg
    24541 aacgttttcc accatgatga tcgacgcgtc caccatggcc ccgatcgcga ccgcgatgcc
    24601 cccgagcgac atgatgttcg cggtgaggcc ctgcgccgac atgggaagga acgagagcac
    24661 gacggcgatc gggagcgtga ggatcgggac gagcgcgctc cggaagtgga agagaaaggc
    24721 gacgatcacg aggctcacga tcgccatctc ctcgaggagc gatcgccaga gcgtgtgcac
    24781 cgcggcccgg atcagctcgg agcgatcgta cgtcgtgacc aggcggacgc cgggggggaa
    24841 ggagccctcc acttcggcga ggcgccgctt cacgccgtcg atcacatgga gcgcgttctc
    24901 cttctgccgc atgatcacga tgccgccgac gacctctccc tggccgttca gctcggcgat
    24961 gccgcggcgc ggctcgggtc ccagatagac ctcacccagc tgggccacgg tcacgggcac
    25021 gcccccttcg cccgtcttca ccacgatgcc gcggaggtcg tcgagcgagc ggatgtagcc
    25081 ccggccccgg acgaagttct cgtgctcccc cacctcgaga acgctcccgc cgacgtcctt
    25141 gttcgagacc tggatcgcgt tcgcgacctc gcggatggag acgccgtagg actcgagccg
    25201 ccggggatcg aggtcgacct ggtactggcg cacgaagccg ccgaccgccg cgacctcggc
    25261 cacgccgtcc acgctctgga gccagtagcg gaggtgccag tcctggaagg agcggagctg
    25321 gctcaagtcg ttctgcccgg tggtgtcgac gagcgcgtac tcgaagaccc aaccgaccga
    25381 cgtcgcgtcg ggcccgagcc gcggggtcac gccctcgggg agctttccct ggagcgaggc
    25441 cagatactcg agaacccggc tccgagccca gtagaggtcg acgccgtcct cgaagaccac
    25501 gtacacgaag gagtcgccga ccatgctgag cccgcgcacg tagcgcacgc ccggcgcgga
    25561 gcggagcgcg gtcacgatcg gataggtgac ctggtcctcg accaggtccg ggcttcgccc
    25621 ggtccactcg gtcgagacga tgacctgcgt gtcggagagg tccggaatgg cgtcgagtgg
    25681 agtgtgccgg agcgcgcgga gccccgccgc cgccgcgacg agaacgagcg cgatcgtgag
    25741 gatccggttc cgcgcgctcc acgtgatcag ccgctcgatc atgatcgcgc taccacttgg
    25801 tcgtcgccga gcggagccgc gcttcggcag ccacgaggaa atttccggac gtgaccacca
    25861 catccccctc gcggaggccg cccacgactt cgtagcggtc gccggccttg gcgccgagcg
    25921 tgacctcgcg cggggcgagc cgcccctgcc ccagatcgac gaacacgatc ctccggtcgc
    25981 ccgaatagag gatggcgctc tcgggaatcg cgagcttccg tccgagatcc cgctcgagga
    26041 ggacgtcgac gaacatgccc ggcttcagat cgccgcgcgg gttcggggcg accgcgcgga
    26101 cctcccccgt ccgggtgtcc gggtcgagga agggattcac gtaggagacg cgaccttccc
    26161 gggcgggctc gcccggaaac ggcggaagga tgcgcgccgt catccccgtc ttcacgaggg
    26221 gaagctcgta ctggaagacg ttcgcgatca cccagaccgg gtggatggga gcgatcttgt
    26281 agagcgtctg ccccgcggtg aacgcgctcc cttggacgac gttcttctcc accaccacgc
    26341 ccgtgaccgg cgcgaggatc gggagcttct ccatcggctt gccggcggat gcgatttcgt
    26401 cgagcgtgga cggggcgatg tcccagagga ggaggcgctg ccgcgccgcg gctgtgagat
    26461 ccccgcccat gggatcgcgg cgcgcttcga ggtattcctg ctgcgcggag aggagctccg
    26521 gactgtagac cgtgaagagc acctcgccgc ggctcacctg ccggccgaca tagtcgacgt
    26581 ggatctcacg aatccagccc gagaacttga gcgagatctc ggcccgccgc gtctcgtcgt
    26641 acgtgacgcg tcccgcggcg cggatcgtcg ccttgaggtc ctgcacggtc gcgggctcgg
    26701 tccgaatgcc gatcgcttgt cgccgggccg gatcgatgat gacctcggcc ccgagtgtcg
    26761 cggcgttcgc gtccaaggcc gcgggcgctc ccgccgccgg cgatccgccg acgaacgagg
    26821 cgccggtcat gttcgtgctc acgcggtaga tcgcctcggc atcggggctg cccgagacat
    26881 ggatcgagat cgcgacgtcc cagtccccct gcatctggag gccgtagctc gcctcgtaga
    26941 tgcccgcgcc cgtctcgcgg aactcgcccc ggctctccat gcgcggcatc gcgcccatcg
    27001 ccggcatcga cacgatcgcg gccagggacg cgcccgacaa aggccgcccc gccgagtcaa
    27061 agagggcgac cttgaacgcg ttgtcaccca cgaccggccg ctcgggccgg gctgcgagcg
    27121 agatctggaa cgcgccgacc cgctgcgtcg ccgccgcggc ggtctcggtg gagcgcgagg
    27181 tctttgtctc gtgcttcgag cagccggcga gtgccacgag ggcgatcccg atgagaaccc
    27241 agcgccggct catcgcggca cctcgacggc cggacccgcg tcgctcgcga tggcgcgctc
    27301 gtagtcggcc agagcgagac ggtacgccac gcgcgcgcgg tgtccgtcca gccgggtgcg
    27361 cgccaggtcc cgctcggcgt tgagcagcgc ggcgaagtcg ctccggttcg attcgtacgc
    27421 gctacggatg gacgcgaggg cgcgctccgt cgcgggcagg acactcgact cgacgacgtg
    27481 gatctcgtgg cgcgtctcct gaacgcgtgc gagcgcctcc tcgatctcga ggcgcgcacg
    27541 atcgatcacg gcgagccgct cctgctcgcg gcgggcgagc ccggcccgag cttcgcgctc
    27601 ggatccgctc acgcggccga agagaaacgg aaggctcatc tcggcgccga ccgtgggacg
    27661 catctcgtgc tccgcccaca tggcgtcgta gcgcgcgacg agcgtgagct cgggaatccg
    27721 tcccttccca gccagctcga gctccgcggc tcgggcatcg cgctccgctt cggctcgctt
    27781 cacgtccggt ctcgacgagg aagcgctggg acccgcgaac ggctcatccc cttcttgggg
    27841 ggccgggatg gaatcgagcg gcgccggcag gtactcctcg ggcgcgcgat ggagcacggc
    27901 gttgagtcgc gcgcgcacga tgcgacggtc ccgcgcgagg acgatcgctt cgtggtcgag
    27961 catcgcgagc tccacctcgg cctggagggc gtcctgttgt ccaaccacgc ccgaggcata
    28021 tttctgtacc gccagggagc ggatgtgacc cagcaggtcc ttctgctcct ggttgacctc
    28081 gagcccgcgc tccaccaggt agaactcgta gtaaaggcgc cgcgtctcgc gaaggagatc
    28141 gagccgggcg gccctctgat cctcgcccgc tgcgcgcgcc tccgcgcgcg cggccttctt
    28201 ctcgagtccg cgcgtgcccc agagcggaag gctctgcgag agaccgatct ggtaccccgg
    28261 cgccgcgaca tcctcgttcc cgatggcttg gggagcgacc atcccgtcga gcaccggact
    28321 catgaagcgt ccggtggcgt c
//
LOCUS       DASTKC010000184         9891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1805343, whole genome shotgun sequence.
ACCESSION   DASTKC010000184 DASTKC010000000
VERSION     DASTKC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805343"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VFR25_07560"
     CDS             complement(<1..1070)
                     /locus_tag="VFR25_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9940943.1"
                     /translation="MPPAAFDEYQELKRRLHREMIGKLNMALLDGLDENGRRDQVESV
                     ARRLLADSELRLTRSDEERLVQELLHDTFDLGPITPYLLDEEISDILVNTHRQVYVER
                     LGRLEMTPVQFRDEAHLRLIIDRIISRVGRRIDESSPLVDARLPDGSRVNAIIPPAAL
                     DGAILSIRRFRRKALSIENLLELGSLTEPMARLLRGIIRARINMLITGGTGSGKTTLL
                     NILSRYISDGERIVTIEDSAELQLQQPHVVRLETRPPNIEGRGTITQRDLVRNALRMR
                     PDRIVVGEVRGDEVLDMLQAMNTGHDGSMTTIHSNGPRDALHRIENLVLMAGHQLHEK
                     AIREQIASALELIVHVSRLADGT"
     gene            complement(1160..2443)
                     /locus_tag="VFR25_07565"
     CDS             complement(1160..2443)
                     /locus_tag="VFR25_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940944.1"
                     /translation="MSLDSHNGNPNPPGGRNGGPTPNGPGRPEAVTILLILEDESNRR
                     STRTALNGLAGVEIVGERFELRSGLLLARQRRPRIVLLDLPDSYEEALNAAGQFKLDL
                     PDTALFLLSNNLDPQLLLRAIRAGAQEVLKKPLDRALLQEAVERVSRSGAARSAAGTR
                     ARSITTLFSTKGGIGTTMVAANLAVSMKAHLNVDTVALADFDAQSSGAAHVLGMKADK
                     SIGDLLRAPRLDSTALQMNLLRHSSGLAVLAQPEDLSSFEAMTPAQAGGVIDALSSSH
                     DAVVVDAPHSFDEVSLEVLDRSNTIVLLAELSIPSIRAARRALEVFDRLHFTDVPDRV
                     KVVVNRYTGTRGFITLDQLFEALQVRAFHTIQNDYRRVFASVNAGRALCLDDPDAQAS
                     KDIIALAAKLVGREVPTTPAEESIRRGLFGRKVLR"
     gene            complement(2453..3856)
                     /locus_tag="VFR25_07570"
     CDS             complement(2453..3856)
                     /locus_tag="VFR25_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET9940945.1"
                     /translation="MKRSPATHRQDERGVAILYVAVFLLVSLWFVSLAIDVGKVMAAR
                     TELQAAADAAALAAASAIDPDTGEIVPDSARVRASVTAFQNKAYQGVETAISIDPDVD
                     VDFPAANQVRVTVHREGSTGNPVILHFAQTLGLPSIGVRADATAEAQELSKVCEHLAP
                     FAPELPPGGDFYENCDSVYTLKMGSQGIASAPTPTPTVELAGFGMFQGSPVLFATAGS
                     AGPAWLAQSGNGKGNGNGGTGGNGNGNGNAGGNGNGNGGGNGNGGGNGGGGGNGGGGG
                     GGNGGGNGGGGGNGGGGGGGGGGGGGGGGGGGGNGGGGTGGNFQLLNYPDCNEDDFTG
                     GGAAAVYEYTKNGYQCCLGIGDEMVLTAPGNKVGPFKSALQERWDADTDQSSSCYQDY
                     TGNQNRVFLTPIVDSFDVNGAKYVRIVKFAAFFLRERPGGDMVQNGVTGQFIKYIAPG
                     EAGPPADTGIYGVHLVE"
     gene            complement(3870..4898)
                     /locus_tag="VFR25_07575"
     CDS             complement(3870..4898)
                     /locus_tag="VFR25_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HET9940946.1"
                     /translation="MDSDQQQAKRERGIAVLFVALFLLVALWFVSMAIDVGKVMAART
                     ELQAAADAAALAGASAIDPVTGVIDQPLAKARAAAAAASNKAYQSTETAVVIDPEVDV
                     EFPQPDRVKVTVKRTEDTGNPVVLHFAQTFGMPTIGVQADATAEAQELNSICEGMAPF
                     APTNPPGGVGYFTDCDSSYALKVGAGNSSQGNFQLLDYPECNEDDFSGSGGAAVRYYT
                     EHGYQCCVNLGQTWLLTEPGNKVGPLQQGLQARWDEDTDKTSHCYEDYHGNGKRIFIT
                     PIVETFDVNGKKNVRIVNFAAFFLNYRPQGSMVQQGVRGQFIQYVAPGGVSGPPVDTG
                     IYGLHLVE"
     gene            complement(4908..6089)
                     /locus_tag="VFR25_07580"
     CDS             complement(4908..6089)
                     /locus_tag="VFR25_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET9940947.1"
                     /translation="MVRSQTQRKRNGWAGMPVALVAILVLGLVPLFAVSNANAAGSAF
                     PVNVNVGQSVVLEMPAEVTTVSIADPKVADAAVGSQKTVVVNGKEAGVTSLVVWEEGG
                     HYTLYQIICTNPGKRQQVLLKVRIGEINTTKAKQLGMDWTTYIRSAKHLDGAIAGGLF
                     TGKIESPSDPPVLGPVVDGFAAYRKTSGDFQFLTIFHALEEHGLARTLASPNLVALSG
                     DSASFIAGGEFPVPITRSSSDNTVAFTIEWKQFGIRLQCLPTVLDSNRVQLYVEPEVS
                     APDYQHAVLLAGSLIPGIITRRVSTTVELKSGEVLVIGGVKSTESIKQVSKFPILGEI
                     PILNLLFKHTRTDLAERDLVMIVSPEILGSTARSWPSPIPGEEKYGNPQGGTAQPPQT
                     K"
     gene            complement(6130..7017)
                     /gene="cpaB"
                     /locus_tag="VFR25_07585"
     CDS             complement(6130..7017)
                     /gene="cpaB"
                     /locus_tag="VFR25_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9940948.1"
                     /translation="MARSKSILALAVGLGFLAALAGYAGLKATAAQVAQNTSKHFVPV
                     VVAATDLTFGTKLDRAQLRVAKYPKESLPSGAFSDIDSVVGQTTKIFLAGKEPITATK
                     LSSRGGGLSMLVRTGFRATSLEVNQVSGVSGFVLPGDRIDVLATVDGRGADQEAVTRT
                     ILQNAEVLASGQKTTQQDNKPITVQAVTILVDPMGAEKLALALHEGKLHLVLRNPEDS
                     DTTKVLSLNTNEMLNGQAGTMPRSTAHAKARKAPQQSAAVPKQPEVRTVQQRPSKPVV
                     RIIREAKVNEQAAPKDSIQ"
     gene            complement(7043..7456)
                     /locus_tag="VFR25_07590"
     CDS             complement(7043..7456)
                     /locus_tag="VFR25_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET9940949.1"
                     /translation="MHPGLAPERRSESGVALIEFAFVLPILLVLAMGMLDFGRAFHEK
                     SLLDQAAREGARVAVVTSPDVDIVEDHVNAVLASGGIVPTSVTVDGPDAAQMVTVTVN
                     ATFTFITPGVFLFLPGWDTGATIPMTGQTVMRFEG"
     gene            complement(7477..8004)
                     /locus_tag="VFR25_07595"
     CDS             complement(7477..8004)
                     /locus_tag="VFR25_07595"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET9940950.1"
                     /translation="MNSWMTGGAVLLCGIATWWDLRYRRIPNLLTLPALGLALCLHGA
                     TEAGPGLLLSAGGALAAGALLLPGYVMRFTGAGDVKLLMAVGAFLSFPQALMAGLLAL
                     VLGGVLGLLTALRFGRLGHVLTRSFGLARWLAHRAKGSPLARPMPSGLRVPFGVAIAL
                     ATVLIVLVPGLGGSR"
     gene            complement(8032..8214)
                     /locus_tag="VFR25_07600"
     CDS             complement(8032..8214)
                     /locus_tag="VFR25_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017243521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET9940951.1"
                     /translation="MLQFAKGFIKDERGEDLIEYGLLAAFVAAIAVAVIIADPLGLMG
                     AVEGAYQSAVDALNGM"
     gene            8605..>9891
                     /locus_tag="VFR25_07605"
     CDS             8605..>9891
                     /locus_tag="VFR25_07605"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9940952.1"
                     /translation="MNLDKAFAYIDQNQKRLIDELIQFLRIPSISAHKDRDADVARAL
                     EYDKKKLESLGFKTEVWPTRAHAGLFAERMVSPDLPTVLIYGHVDVQPVDPLDLWTSP
                     PFEPRIADGRIWARGADDNKGQHYAQIAGVEAALKGGGELPVNVKFILESDEEHDSEA
                     MAMELEKHKDKLACDVFVVSDSNFPDRDHPAVTLSLRGVLAMEVTITGAAGDKHSGEW
                     GGMLYEPIDVLRWVLHNLKDFETGKVLVPGFYDDVDEPTPATRAAIAGRPWDDAAKAK
                     EQGVKRLFNEKGYTALESGTLRPTLQTNGIYGGYMGEGFKTVIGNRATAKISTRLVPR
                     QNPDKIFAAIERRIRELVGDKGTVEIRKFGGGLPFWADPEDPYVKAGLRALTTAFGKP
                     SVVLAMGGSIPFVPHLVRVTGAPCVMMGFGLPDDNLH"
BASE COUNT         1605 a         3270 c         3336 g         1680 t
ORIGIN      
        1 gtgccgtcgg ccagtcgcga gacgtggacg atgagctcga gggccgatgc gatctgctcg
       61 cgaatcgcct tctcgtggag ctggtgcccg gccatgagaa ccaggttctc gatgcggtgc
      121 agcgcgtcgc gcggaccgtt cgagtggatc gtggtcatcg agccgtcgtg accggtgttc
      181 atggcctgga gcatgtccag cacctcgtcg ccgcgcacct cgccgaccac gatgcggtcg
      241 ggacgcatac ggagcgcgtt tcggacgagg tcgcgctgcg tgatcgtgcc gcgcccttcg
      301 atgttcggag gacgggtctc gagccgcacg acgtggggct gctggagctg gagctccgcc
      361 gagtcctcga tcgtgacgat gcgctcgccg tccgagatgt agcgcgagag aatgttgagg
      421 agcgtcgtct taccggagcc cgtgcccccc gtgatgagca tgttgatccg cgcgcggatg
      481 atgccgcgga ggagccgggc catcggctcc gtcagcgagc cgagctcgag caggttctcg
      541 atggagagcg ccttccggcg gaagcggcgg atcgagagga tcgcgccgtc cagcgccgcg
      601 ggcgggatga tcgcgttcac gcgcgacccg tccgggaggc gcgcgtcgac cagcgggctc
      661 gactcgtcga tgcgtcggcc gacccgcgag atgatgcggt cgatgatgag acggaggtgc
      721 gcttcgtcgc ggaactgcac tggagtcatc tcgaggcgcc cgagccgctc cacgtagacc
      781 tgccggtgcg tattgacgag aatgtccgag atctcctcgt cgaggagata cggcgtgatc
      841 ggtccgagat cgaacgtgtc gtggaggagc tcctgcacga gccgctcctc atcggaacgg
      901 gtcagacgga gctcggaatc ggccaggagc cgccgcgcga ccgactccac ctggtcacgg
      961 cgcccgttct cgtccaggcc gtccaagagc gccatgttga gcttgccgat catctcgcga
     1021 tgaaggcggc gcttcagctc ctggtactcg tcgaacgccg ccggaggcat cgcattcggt
     1081 cgccggtcag cgggctcggt ttcggtcgtg gtggaggcag gactgcccgc cgcacccttg
     1141 cggaaacgat ctcgtaagct catcgaagca ccttccttcc gaagagaccc cgccggatgc
     1201 tctcctccgc cggcgtcgtg ggcacctcgc gtccgaccag cttggccgcg agcgcgatga
     1261 tgtccttgga tgcttgcgcg tcgggatcgt cgaggcagag cgcccgaccc gcgttcacgg
     1321 acgcgaagac gcgtcgatag tcgttttgaa tcgtgtggaa cgcccggacc tgaagcgcct
     1381 cgaagagctg gtccagggtg atgaacccgc gcgtgccggt atagcggttc acgacgacct
     1441 tcacgcggtc gggaacgtcg gtgaagtgga gcctgtcgaa gacttcgagg gcgcgccgcg
     1501 ccgcccggat cgatgggatc gagagctcgg ccaggaggac gatcgtgttg ctccggtcga
     1561 gcacttcgag cgacacctca tcgaacgagt gcggggcgtc cacgacgacc gcgtcgtgcg
     1621 aggaggagag cgcgtcgatc acgcctcccg cctgcgccgg cgtcatcgct tcgaagctcg
     1681 agagatcctc gggctgcgcc agcacggcga ggccgctcga gtggcggagc aggttcatct
     1741 ggagcgccgt ggagtcgagc cgcggcgcgc ggagaaggtc cccgatggac ttgtcggcct
     1801 tcatgccgag cacgtgggcg gccccgctcg actgcgcgtc gaagtcggcc agcgccaccg
     1861 tgtccacgtt caggtgagcc ttcatcgaga ccgccaggtt tgccgccacc atggtcgtcc
     1921 cgatgccccc cttggtgctg aacaaagtgg tgatcgagcg cgctcgggta cccgcagcgg
     1981 aacgggcggc gccgctccgt gagacgcgct cgaccgcttc ctggaggagc gcccggtcga
     2041 gcggcttctt caggacctcc tgcgctccgg cgcggatggc gcggaggagg agctgtggat
     2101 ccagattgtt cgagaggagg aagagcgccg tgtcgggcag gtcgagcttg aactgacccg
     2161 cggcgttgag cgcctcctcg tagctgtcgg ggagatcgag caagacgatg cgcgggcggc
     2221 gctgcctcgc gagcaggagt ccgctccgga gctcgaagcg ctcgccgacg atctcgacgc
     2281 ccgcgaggcc attcagagcc gtgcgcgtgc tgcggcgatt cgactcgtcc tcgaggatga
     2341 ggaggatcgt cacggcttcc ggacgccccg gaccgttcgg cgtaggaccg ccgttacgcc
     2401 cgcctggcgg gttcgggttc ccgttatggc tgtcgagcga catggcctct ccctattcga
     2461 cgaggtgaac gccgtagatc ccggtgtcgg ccggaggacc ggcttccccg ggagcgatgt
     2521 acttgatgaa ctgtccggtc acgccgttct ggaccatgtc gccgcccggg cgctcccgga
     2581 ggaagaaagc cgcgaatttc acgatccgca cgtacttcgc gccgttcacg tcgaagctgt
     2641 cgacgatcgg tgtgaggaac acgcggttct gattgcctgt gtagtcctgg tagcagctcg
     2701 atgactggtc cgtgtccgcg tcccagcgct cctggagcgc cgacttgaac ggaccgacct
     2761 tgttgccggg cgcggtgagg accatctcgt caccgatccc gaggcagcac tggtagccgt
     2821 tcttcgtgta ctcgtagacg gccgcggcgc cgccgccggt gaagtcgtcc tcgttgcagt
     2881 ccggatagtt gaggagttgg aagttgcctc ccgtaccgcc gccgccgtta ccgcctcccc
     2941 cgccgccgcc accaccaccg ccgccaccgc caccaccgcc accaccgcca ttgccaccgc
     3001 cgccgccgtt gccgccgccg ttgccaccgc caccgccacc gccgttgcca ccgccaccgc
     3061 cgttgccacc gccattgccg ttgccaccac cgttaccgtt tccgttgccg ccggcgttac
     3121 cgttgccgtt gccattgccg ccggtgccac cattgccatt gcctttgccg ttgccgctct
     3181 gcgcgagcca cgcgggcccc gcggagcccg cggtcgcgaa gaggacaggg ctgccctgga
     3241 acatgccgaa gccggcgagc tccaccgtgg gagtcggcgt cggcgcgctc gcgatgccct
     3301 gcgaacccat cttcagcgtg tagacgctgt cgcagttctc gtagaagtcc ccgcctggag
     3361 gaagctccgg cgcgaagggg gcgaggtgct cgcagacctt gctcagctcc tgagcctccg
     3421 cggtcgcgtc ggcgcgcacg ccgatcgacg gcaaaccgag cgtctgggcg aagtggagaa
     3481 tgaccgggtt gcccgtcgat ccctcgcggt gcacggtcac gcgcacctgg ttcgcggccg
     3541 ggaagtccac gtccacgtcg gggtcgatgg agatcgccgt ctcgacgcct tggtacgcct
     3601 tgttctggaa ggccgtgacc gaggcgcgca cccgtgcact gtcgggcacg atctcgcccg
     3661 tgtccgggtc gatcgcggag gccgccgcga gcgcggcggc gtcggccgcg gcctggagct
     3721 ccgtccgggc ggccatcacc ttgcccacgt cgatggcgag cgagacgaac cagagggaga
     3781 cgagcaggaa gaccgcgacg tagagaatcg cgacgccgcg ctcgtcctgc cggtgggttg
     3841 cgggtgaccg cttcatgccg ccttcctctc tattcgacaa gatggagccc atagatcccg
     3901 gtgtcgaccg gcgggccgct cactccgccc ggggccacgt actggatgaa ctgccctctc
     3961 acgccctgct gcaccatgct tccctgcggc cggtagttca ggaagaaggc cgcgaagttc
     4021 acgatgcgga cgttcttctt gccgttcacg tcgaaggtct cgacgatcgg cgtgatgaag
     4081 atccgcttcc cgttgccgtg atagtcctca tagcagtggc tcgtcttgtc cgtgtcttcg
     4141 tcccaccggg cctggagccc ctgctggagc ggccccacct tgttgcccgg ctcggtgagc
     4201 agccacgttt gtcccaggtt cacgcagcac tggtagccat gctccgtgta gtaccggacc
     4261 gccgcgcctc cgctccccga gaagtcgtcc tcgttgcact ccgggtagtc gaggagctga
     4321 aagttgccct gactcgagtt tcccgcgccc actttgagcg cgtagctgct gtcgcagtcg
     4381 gtgaagtacc cgaccccacc cggcggattc gtcggggcga agggcgccat gccctcgcag
     4441 atcgagttca gctcctgcgc ctccgcggtc gcgtccgcct gcactccgat cgtgggcatg
     4501 ccgaacgtct gcgcgaaatg gagcaccacc ggattgcccg tgtcctcggt gcgcttgacg
     4561 gtcaccttca cgcgatcggg ctgcgggaat tccacgtcca cctccgggtc gatcacgacc
     4621 gcggtctcgg ttgattggta cgctttgttg ctggctgctg ccgcggccgc ccgcgccttc
     4681 gcgagcggct ggtcgatcac tcctgtgacg gggtcgatgg cgctcgcgcc ggccagggcc
     4741 gccgcgtccg cggccgcctg aagctcggtg cgagccgcca tcacttttcc cacgtcgatc
     4801 gccatcgaga cgaaccagag ggctacgagg aggaagagcg ctacgaacag aaccgcgatc
     4861 cctcgctcac gtttcgcctg ctgctggtcc gaatccacga atggctccta tttcgtctga
     4921 gggggttgcg ccgtgcctcc ctgggggttg ccatacttct cctcgcccgg gatcggagac
     4981 ggccagctgc gggccgtcga gccgaggatt tccggcgaga cgatcatgac cagatcccgc
     5041 tccgcgagat ccgtacgggt gtgcttgaac aagaggttca ggatcggaat ctcgcccagg
     5101 atcgggaact tcgacacctg cttgatggac tccgtgctct tcacgccgcc gatcacgagg
     5161 acctcgcccg acttgagctc gaccgtcgtg gacacgcgcc gcgtgatgat gccggggatg
     5221 agcgacccag cgaggagcac cgcgtgctgg tagtcgggcg cgctcacctc gggctccacg
     5281 tagagctgga cccggttcga gtcgagcacg gtcggcaagc actggagccg gatgccgaac
     5341 tgcttccact cgatcgtgaa cgcgacggtg ttgtccgagc tcgaccgcgt gatcggaacc
     5401 gggaactcgc cgcccgcgat gaacgaggcg ctgtcgccgc tcagagccac caggttcggg
     5461 ctcgcgagcg tacgcgccag gccgtgctcc tcgagcgcgt ggaagatcgt gaggaactgg
     5521 aagtcgccgc tcgtcttcct ataggccgcg aatccgtcga ccaccggccc taggaccggc
     5581 gggtcggacg gggactcgat cttgccggtg aacagaccgc ccgcgatcgc gccgtccagg
     5641 tgcttggccg agcggatgta ggtggtccag tccatcccga gctgcttcgc cttggtcgtg
     5701 ttgatctcac cgatccgaac cttgaggagc acctgctggc gcttgccggg gttcgtgcag
     5761 atgatctgat acaaggtgta gtggccgccc tcttcccaga cgacgaggga ggtgacgccc
     5821 gcttcctttc cgttcacgac cacggtcttc tgcgagccca cggccgcgtc ggcgaccttc
     5881 gggtccgcaa tcgagaccgt cgtgacctcg gcgggcatct cgagcaccac cgactgcccc
     5941 acgttgacgt tcaccgggaa ggcggagccg gccgcgttcg cgttcgagac cgcgaagagg
     6001 ggcaccaatc cgagtaccag gatggcgacg agcgccacgg gcatcccggc ccatccattt
     6061 cgctttcgct gcgtctgcga gcgaaccatc ttgcagctcc tgacgttcgg gtggacgact
     6121 taccgcttct tactgaatgg aatccttcgg ggccgcctgc tcattcacct tggcctcgcg
     6181 gatgatccgg accaccggct tgctcggccg ctgctgcacc gtgcgcacct ccggctgctt
     6241 cggcacggcc gctgactgct gcggcgcttt cctggccttc gcatgggccg tgctgcgcgg
     6301 catggtgccg gcctggccgt tcaacatctc gttcgtgttg agcgagagca ctttcgtcgt
     6361 gtcggaatcc tccggattgc ggaggacgag gtggagcttg ccctcgtgga gcgcgagcgc
     6421 gagcttctcg gcgcccatcg gatcgacgag gatcgtgacc gcctggaccg tgatcggctt
     6481 gttgtcctgc tgggtcgtct tctggcccga cgcgaggacc tcggcgttct ggaggatcgt
     6541 gcgggtcacc gcttcctgat cggcgccgcg gccatccacc gtcgcgagga cgtcgatacg
     6601 gtcgcccggg agcacgaagc cgctgacgcc ggagacctgg ttgacctcga gggacgtggc
     6661 gcggaagccc gtccggacga gcatcgagag accgccgccg cgcgaggaga gcttggtcgc
     6721 ggtgatcggc tccttgccgg cgaggaagat cttggtcgtc tggccgacga ccgaatcgat
     6781 gtcggagaag gcgccgctcg gaaggctctc cttgggatac ttcgcgacgc ggagctgcgc
     6841 gcgatcgagc ttcgtcccga aggtgagatc cgtggcggcg accacgaccg gcacgaagtg
     6901 cttggatgtg ttctgcgcga cctgggccgc cgtggccttc agaccggcgt acccggccag
     6961 agccgcgagg aaacccaggc ccacggcaag tgcgagaatt gacttggagc gggccatgag
     7021 ttctatccct ccttcgtgta ggtcagcctt cgaaccgcat caccgtctgg ccagtcatcg
     7081 gaatcgtggc cccggtgtcc catccgggca ggaacaggaa cacgcccggc gtgatgaacg
     7141 tgaaggttgc gttgaccgtt accgtgacca tttgtgccgc gtccggaccg tccacggtga
     7201 cggatgtcgg aacgatcccg cccgaggcga gaacggcgtt gacgtgatcc tcgacgatgt
     7261 cgacatccgg gctcgtcacg acggccacgc gcgctccctc gcgagcggcc tgatcgagca
     7321 ggctcttctc gtggaaggcg cgtccgaagt ccagcatgcc catcgccagc accaggagta
     7381 tcggcaggac aaaagcgaac tcaatcaacg ccacgcctga ttctgaacgg cgttccggag
     7441 ccaggccggg atgcaccgac cgctccctac gggagctcat cgagaacctc caagtccggg
     7501 caccagaacg attaagacag ttgcgagcgc gatcgcgacg ccgaacggca cccggaggcc
     7561 ggacggcatg ggacgcgcga gcggagaacc cttggcgcga tgagccagcc atcgcgcgag
     7621 tccaaaggag cgagtgagaa cgtggcccag ccggccgaac cggagagccg tgagaaggcc
     7681 gagcaccccg ccgaggacga gggcgaggag tccggccatc agcgcttgcg gaaaggagag
     7741 aaaggctccg accgccatca ggagcttcac gtctccggcg ccggtgaagc gcatcacgta
     7801 gccgggcaag agaagcgcgc cggccgcgag tgctccgccc gcggagagga gaaggcccgg
     7861 cccggcttcg gtcgccccgt gcaagcacag ggcgagtccg agagccggga gggtcaggag
     7921 attcgggatc ctgcgatagc gcaggtccca ccacgttgcg atgccacaca ggagtaccgc
     7981 gccgccggtc atccaggagt tcatggcacg ctccgtggtt ggtgatttga attacatgcc
     8041 gttgagggcg tcgaccgcgc tctgatacgc gccttcgacc gcgcccatga ggccgagcgg
     8101 atccgcgata atcacggcca ccgcgatggc agccacgaac gccgccagaa ggccgtactc
     8161 gatgagatcc tcgccgcgct cgtccttaat gaaacccttg gcgaactgaa gcatggtgaa
     8221 accctccact aatcggtgtg ctgcgttgtt gtccgctctc ggtccgactt cacttggggt
     8281 cggactctcc cgatgcggac ggctcacgat cggctgcatc gaacgccccc gccgcgggga
     8341 cccgctttcg atgcagtcga ctagggcatt cgcaagcgcc gtgccgagtt gtaccgaggc
     8401 gaaggagcgc gcagtgatgt cgcgtgcgtg taagcgcttg gacggtgtga gtcgctacat
     8461 gaaggcgcgc gagcgcgata tgagaggcgc gtggatgggg gttttccccc atggttcctg
     8521 cggattgtgg ccacgcccca accgcgctag gatggtcgcc gcgaatcgcg atgtgtgttg
     8581 cagaacgcca ccttgggagc tctcatgaac ctcgacaagg ctttcgccta catcgaccag
     8641 aaccagaagc ggctcatcga cgagctgatc cagttcctcc ggattccgag catcagcgcg
     8701 cacaaggacc gggacgccga tgtcgcgcgc gctctcgagt acgacaagaa gaagctcgag
     8761 tcgctcggct tcaagaccga ggtgtggccg acgcgcgcgc acgcggggct cttcgccgag
     8821 cgcatggtgt cgcccgatct cccgaccgtg ctcatctacg gccacgtgga cgtgcagccc
     8881 gtcgatcctc tggacctttg gaccagccct cccttcgagc cccggatcgc ggacgggagg
     8941 atctgggccc gcggcgccga cgacaacaag gggcagcact acgcccagat cgccggcgtc
     9001 gaggcggccc tcaagggagg gggcgagctg ccggtgaacg tgaagttcat cctcgagagc
     9061 gacgaggagc acgactcgga agccatggcc atggagctcg agaagcacaa ggacaagctg
     9121 gcgtgcgacg tgttcgtggt ctccgactcc aacttcccgg atcgcgacca cccggccgtg
     9181 acgctctccc ttcgcggggt cctggcgatg gaggtcacca tcacgggagc ggcgggggac
     9241 aagcactcgg gcgagtgggg cggaatgctc tacgagccga tcgacgtgct ccggtgggtg
     9301 ctccacaacc tgaaggactt cgagacgggg aaggtgctcg tccccggctt ctacgacgac
     9361 gtcgacgagc ccactcctgc gacgcgcgcc gccatcgccg gccgcccgtg ggacgacgcc
     9421 gccaaggcga aggagcaagg ggtgaagcgg ctcttcaacg agaagggcta cacggcgctc
     9481 gagtcgggca cgctccgccc cacgctgcag accaatggga tctacggcgg ctacatgggc
     9541 gagggcttca agaccgtgat tggaaacagg gccaccgcca agatcagcac gcggctcgtc
     9601 ccacggcaga acccggacaa gatcttcgcc gcgatcgaac ggcggattcg cgagctggtc
     9661 ggcgacaagg gaaccgtgga gatccggaag ttcggcgggg gacttccctt ctgggcggat
     9721 ccggaggacc cgtacgtcaa agcagggctc cgcgcgctca ccacggcgtt cggcaagccc
     9781 tcggtcgtcc tcgccatggg gggctccatc ccgttcgtcc cgcacctcgt gcgtgtgacg
     9841 ggggcgccgt gcgtgatgat ggggttcgga ctgcccgacg acaacctgca c
//
LOCUS       DASTKC010000185        23014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_150752, whole genome shotgun sequence.
ACCESSION   DASTKC010000185 DASTKC010000000
VERSION     DASTKC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 23014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23014
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150752"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFR25_07610"
     CDS             complement(<1..334)
                     /locus_tag="VFR25_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET9940953.1"
                     /translation="MKAAVIGGGPAGLFFALLLKKAEPKHEVQVYERNRLEDTFGFGV
                     VFSDATEAALEHADPEVIAEMGARCHRWDDIEIHYTGMVLRSTGHGFSGLSRKTLLAL
                     LADRCRASG"
     gene            complement(331..1482)
                     /locus_tag="VFR25_07615"
     CDS             complement(331..1482)
                     /locus_tag="VFR25_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9940954.1"
                     /translation="MSHPDPIPVRAFLDDAHQALAPVVGAFAGAAARELPEPLDDAEG
                     RTRARAWLSRLGEGGLLDPIRRQDWRGCCLTREALGAASPLADAVFALQGLAAVPLHF
                     AENEAMRSRWLEDAVSGRAMGAFAMTEPEAGSDAAAIATRARRDGDAYVLDGTKTLIS
                     NAGIADFYTVFATTDPSLGAKGISCFVVPAETPGLTFTRAQVLSAPHPLGEIAFEGCR
                     VPEGNRLGPEGRGLALGLKTLDRLRATVGAAACGMAARALDEALRHARTRRQFGKPLS
                     EFQLVQEKLARMATDLTAARLLVYRAAYEADRGAERVTLEAAMAKAFATEAAQRVIDD
                     AVQILGGSGVLASSPVDRLYRAVRALRIYEGTTEIQHLVIAGQLVKEAG"
     gene            complement(1479..2279)
                     /locus_tag="VFR25_07620"
     CDS             complement(1479..2279)
                     /locus_tag="VFR25_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET9940955.1"
                     /translation="MIQPRSFRFAVDPATRVARITLDRPDRLNALTFEVYAELRDLFA
                     RLDTEPGVGAIVLTGSGRAFCSGGDVEDIIGALFSRDSAGLREFTRMTCDLILNIRRC
                     RRPVIAALNGTVAGAGAVIAAACDVRIASETAKIAFLFVRVGLSGADMGASWLLPRIV
                     GHGRATEILMTGDFVDARRAQEIGLYNAVVPVDRVVDEAVALAARLARGPAEALAVTK
                     RALDAEWALDFESALAHEADAQAALMERPDFREAYEAFRAKREPKFQA"
     gene            complement(2276..2686)
                     /locus_tag="VFR25_07625"
     CDS             complement(2276..2686)
                     /locus_tag="VFR25_07625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9940956.1"
                     /translation="MSLENVNPASLGTPKGYSHGVLGPAGGRVLFVAGQPGWDASEKG
                     PVAGFVQQFARALDRVLAVVREAGGGPADVARMTVYVTDLNSYRVALEPLGRVWRERF
                     DRRYPAMAMVEVSGLVDEGAVVEIEATAVIGGTP"
     gene            complement(2683..3447)
                     /locus_tag="VFR25_07630"
     CDS             complement(2683..3447)
                     /locus_tag="VFR25_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9940957.1"
                     /translation="MKRGRTAVVTGAGRGIGAAIAHALADEGIALLLAARTREEVDAV
                     AADLRARGVEAHAAPCDVTDEASVKALARDAEAKLGKVDILVNNAGVSSSAPLRKLTL
                     DEWNRVLAVNATGVFLCTREFAPAMAERGWGRVITIASTSGLEGGRYIAHYCASKHAA
                     VGFTRAAALELEDRGVTVNAICPAYVDTPMTDRTLATVGAKTGLARDAALRAVLETAG
                     QERLIRPEEVAAAVVRLCRDDAVETGQAIVLNGRGR"
     gene            complement(3444..4214)
                     /locus_tag="VFR25_07635"
     CDS             complement(3444..4214)
                     /locus_tag="VFR25_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET9940958.1"
                     /translation="MTVPRWADLTWEEVRDLPRERLTAILPVGAVEAHGPHLPLGTDL
                     VIAEAMARAGGDCLAGAGIHAVLLPALPYTAAPFGAAFAGTVSMPADIVTGLVAALAR
                     ELKRHGVAALAVANAHLDPAHVGALQEAVDRARRDGSIPIVCPLLTRKPWALRLTDEF
                     RTGACHAGRYEGSIVMAERPDLVRREVMRSLPPNPASLSRAIRDGKGTFEEAGGPRAY
                     FGWPADATDAEGRETIEALGQILCDAVLEAFGSEHATI"
     gene            4285..5805
                     /locus_tag="VFR25_07640"
     CDS             4285..5805
                     /locus_tag="VFR25_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET9940959.1"
                     /translation="MTLEPSERLNIADLFLDERVREGRGARTAIRTDSGTSTYAEVQA
                     LANRFAQALRQAGVQPEHRVILGMPDGVGYVAALFGILKLGGVAVMVNPGLPAAEVAW
                     ILDHARARAIVTHASSAEPFLQSAAVSPFAPKVLVHGDDAFDHTLEAQSDTFPTFPTH
                     RDDPAIWLFSGGTTGRPKAVVQTHRSFANTARLYGGQVIAYTEDDITLSVPKLYFGYA
                     TGSNLFFPFAAGASTALFPEPANAATLFDKIKRHKPTILVQVPTMIHKMLSHPGAAAS
                     DLSSLRLVTSAGEALPAEIDERWRRTFGVEILDGLGTAEMWHVFISNRPGQSRPGTLG
                     TVVPGFEVRVCDEEGRELPRGETGWLWVKGDSRALGYWHDLDKTARSFRGEWYVSGDL
                     ITMDAEGYVTYCGRGDEMLKVAGKWLAPQEVEGCLLEHPSVAEAVVVGVPDAQGLVKP
                     RAYVVPKGAKDGLEETLKAHVRDRLDAYKHPREVVFVDSLPRTHLGKVDRGSLRRR"
     gene            5805..7481
                     /locus_tag="VFR25_07645"
     CDS             5805..7481
                     /locus_tag="VFR25_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940960.1"
                     /translation="MATPISIVPRKRPAPPAAAAVPSTDRQRLPGTRLQVAILAFAVF
                     VVAAIVHSQTLSFRFLTTWDDPSYITNNPWIRGLTLENIRFAFTTPYFANYLPLHLVS
                     YMVDYQLWGLNPFGYRLQSLFLVALNASLAFFLVRRLFRSLALGVIAALLYAVHPAHV
                     EAIAWISIRKDLLSTVFLLFTVILYDEATRDRFRPWVYAISVVMYVLGLLSKVSISTL
                     PLFLFVLDWTRRWDGRAFRWKQSIATKIPYLAFAAVLVYVNNHAQVKTGMPYTHQPLQ
                     FIMVKGEAIWRYLAMLTGMVDGVPMYDPPWLGGLHPPFNLAGALLPFVVLGFAFWRRL
                     RALTLGAAWLFILLIPVLAFPLMVYMADRYLYSPSLGFCWVLAAGILWLAGRVRSQRG
                     RAAAAVVLTAIPFTLFTMRTVRYEALWGNPEALWRYTMQHTKDVRAKSALAQTLTLQR
                     RYDEAEQIYQSLMVYKSTDVYAGLASIYIPTGRFDEAQEMMDQAVLHWAGKRDNEKAS
                     ILCYRAMVEWAREDKDAAIRDWIEAARLDPKNDESRIHLRRLGVEPPASR"
     gene            7484..9148
                     /locus_tag="VFR25_07650"
     CDS             7484..9148
                     /locus_tag="VFR25_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940961.1"
                     /translation="MAEPITIVPRRKRAEPVAGSAASRPVKRDGGLQFWILAATSFGA
                     ALLVHAQTLGFPFLMTWDDPTYVVHNPWIRGLTLENLRFAFTAPYFSNYLPLHLVSYM
                     VDYSVWGLNPLGFRIQSLLLAGMNTALAFALTRRLFGSVALGIVAALLYAVHPSHVEA
                     VAWLSIRKDLLSTFFLLLSVLFYDKATREGFHRAWYATSVATFLLGLLSKVSISMLPL
                     FLLVLDLTRRWDGGAFSWRRSIATKIPYAFFAGALVVVNDLVQVRTHLPYAHQPLSYL
                     MVKGHSVAMYLGLLAGVFDGRPIYDPPSVAGFRGVLDLAALVFPLVVFAFAWSRRSRV
                     LALGTAWIFVLLLPAIAFPLVTYMADRYLYAPSLGFCWILAAGILSLAGKARSERRRV
                     LAVVAMTALALAFFAVRTVRYESVWGVPQKLWAYAHQRSHDVRATSNLAQAMYVNHNY
                     DEAEQLYRTILGTNTVEAWTGIACVYFNTRRYSDAKDAMDHAVLAMGRRRPSQQADVL
                     YLRGQIEWPFDREAAIRDWEEAVRIDPKHERAKASLSAPDAPPSGL"
     gene            9151..10848
                     /locus_tag="VFR25_07655"
     CDS             9151..10848
                     /locus_tag="VFR25_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940962.1"
                     /translation="MAAISRSARRERAKRDAKMGRPAGGAPGARLPGTLLQLALASIA
                     AFAAAYFVHQPSLHFGFLTSWDDPTYVTDNPWIRGLTAENIKFAFTTPYFSNYLPLHL
                     VSYMVDYSLWGLNPFGFHLQSVILAALNAVLAFFVVRRLFGSFALGWVAALLYAVHPS
                     HVEAVAWVSIRKDLLSTVFLLVTLILYDEATQGRKLRVAWYAASVVTFLLGLWSKMSI
                     VALPLFMLVLDFMRDRQGRPFDWKRAIVTKIPYGLLGVWLVIVNNQVQVKSEQPYAHQ
                     PLQYLMVKGHAVWLYLGLLTGITQGRPIYDPPSLSVLDLVGVLILPAIFWLAYRRRLR
                     ALALGAAWVFVLLIPALAFPLITYMADRYLYAPSLGFCWILGAGLVAIASRVKAEPGR
                     TVTLAAITAIPFVFFTVRTREYQSVWRDGEALWTYTTKHSRDFRALNNLAQVRLNQQR
                     YADAERLYGQASKFDNIVSWQGLATTYYNTKRYEQAQAAIDKAVAIMKTKRSSPDEQA
                     ELYYTKGAIEWVRGQNAAAQQDWEEAVRIYPQHAQANAWLRTARGQAPPPPPPTTTH"
     gene            complement(10977..11489)
                     /locus_tag="VFR25_07660"
     CDS             complement(10977..11489)
                     /locus_tag="VFR25_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9940963.1"
                     /translation="MAKTETRAVADIREHLERFRGVTLQTLEWVPEGNLHFAPQEGLR
                     PFDEQFLHIARTEEYHVRGLLLGDWNRERYFTRPDSLTKESLRAILHETRAVTLKALD
                     SLDPARLEDNVQVPDVPVDWTLRAWLWYLVEHEVHHKAQLALYLRLVGVIPPFFALAF
                     PPGVRPDVRG"
     gene            11567..12001
                     /locus_tag="VFR25_07665"
     CDS             11567..12001
                     /locus_tag="VFR25_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9940964.1"
                     /translation="MPVETEEELAAIVKSASVVAVIGIKDGSDPHAPAYSIPKMLQER
                     GIRIVPVHPTLTSVLGERVYPAIGDVPERVDIVDLFRRPSAIDAHADEILALPESKRP
                     GVVWMQTGIRNDAAAARLEAAGIQVVMDRCLGVYSARYRPRA"
     gene            12074..13186
                     /gene="ada"
                     /locus_tag="VFR25_07670"
     CDS             12074..13186
                     /gene="ada"
                     /locus_tag="VFR25_07670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HET9940965.1"
                     /translation="MNAIDERRWQAVLARDPSRDGTFVTAVLTTGIYCRPSCPARHPR
                     PENVRFYPRPDDAEKAGFRPCKRCRPREERRPDRATDLVPRVCRYLDENRDERVTLLD
                     LAREMKMSPHHLQRTFKRATGVSPRQYAEALRLGRLKQRLQGKEPVTMALYEAGYGSS
                     SRLYERSNARLGMTPGAYRAGGKGSVIRYAIASTPIGRVLVAATEKGVCSVRIGEPAE
                     TMERALKREFPEATLRRDRAGLAAWIERIARHLGGRAPHLDLPLDVRGTAFQWKVWEA
                     LQAIPYGETKTYREVARAIGKPRAARAVGRACATNPVAILIPCHRVVREGGALGGYAY
                     GLPTKKRILDTERSSAPASARRRRQTGAGRMPRPIA"
     gene            complement(13147..15411)
                     /locus_tag="VFR25_07675"
     CDS             complement(13147..15411)
                     /locus_tag="VFR25_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9940966.1"
                     /translation="MAIAQESDAAELLRLLVRCIAHLDGFRVGQAYRATPDSDPVWCS
                     AWHSGDSDVERFVQKSRCLGSAGPGSLPARALADGEIRVLNPPDRFLDETRRNEALQA
                     GLEAGFAIPIKVRDRAFAVLEFYRSEPMSPGDPIPLVAEALAAHVGTALALKTAETEC
                     EQLEAGKESERGRAEVVKGELDAMKSIMASVHAELPTIKMVQGCVEALRSGLHVDAVA
                     AFGTRRGSVVPEQLALSSSRNVLPIPGDCSEIVGSMTLGKSPIALERLHGNDQKTLQA
                     IFVAPLVVEEERIGALVLFHHHPKQVTPHMVRLVRITADRIAPRMSRARVIEEAWRDR
                     QELEMLSRKLVTAHESERRAISRDLHDEVGQVIAALKFSFEASGTRSELVQTALASLM
                     QQVRNIAQRLRPSVLDDFGLAPTLEWHARQFSEQTGIRVDFRRFGVCGRYHPDLEIAL
                     YRVVQEALTNAVRHARASRARVVLRTGRGWIGVRVRDYGTGFDSEGIPSGSTGIAGMK
                     ERLRPLRGELVIQSRRGRGTSVTATVPFNPEARIMNEPITVVLADDHRIMREALKVLL
                     EGASFQVVGQAGDGLEAVNVVENAKPSVLLLDVAMPGLGGADVVKQVAKRTPRTRVLM
                     LSMHANDAYVSGSMKNGAAGYVLKDASADELFHAIREVAEGRRYLSRPLEESGVADRD
                     LADAYETLTMREREVLHLAAEGLTSAQIADRLSISPRTAETHRANSLRKLNIRGQTEL
                     VRYAIGRGILPAPV"
     gene            complement(15654..17759)
                     /locus_tag="VFR25_07680"
     CDS             complement(15654..17759)
                     /locus_tag="VFR25_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9940967.1"
                     /translation="MSAPARNRLAREKSPYLLQHAGNPVDWYPWGEEAFAKAAGEEKP
                     IFLSIGYSTCHWCHVMERESFETEEIGRILNEHFVAIKVDREERPDVDHIYMTVCQAL
                     TGSGGWPLTVFLTPAREPFFAGTYFPPETRFGRPGLRELLYQIVGAWESQRDQILTVA
                     TQIVDAIRPNFAAHPGERLDEKTMRAAFEDLAARYDAEQGGFGTAPKFPTPHQLTFLL
                     RYWNRTQDGRALAMVEGTLQAMRRGGIYDQVGFGFHRYATDREWRVPHFEKMLYDQAL
                     LLDAYVEAYQATGQSLYAGVAGEIVTYLGRRLMSPEGAFYSAEDADSEGEEGKFYVWT
                     RDEIDRVLGAERGALYARAYGIEKDGNWVDEAHGTRPGTNIPYLDRDPAALAEELASD
                     PAALALELDGCRAALEEARERRPRPHRDDKILTAWNGLMAASLAKAARALDRPAYAAM
                     AGRALEFVKARLVRGDGRLLARYRDGEAAHAGYLDDYAFVLRARLELFEATSDPAHLE
                     EAVALAREMDRLFWDTAEGGYFFTGQDAEPLLARTKESYDGAIPSGNSVAASALLRLS
                     RMTGDPWGETRGRAVLDAFAGSASSHPTGHACLLGAVDLALGPAQEIVIAGAARDAET
                     EAMVREVGRRFLPRAVLLRAAPETISDLRRWVPALASFPPIEKSGTAYLCEGYHCLAP
                     CSTAHGLAVALQTAGPAAS"
     gene            complement(17756..18865)
                     /gene="mnmA"
                     /locus_tag="VFR25_07685"
     CDS             complement(17756..18865)
                     /gene="mnmA"
                     /locus_tag="VFR25_07685"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9940968.1"
                     /translation="MNDSFLPDHPTTVLVAMSGGVDSAVAAALLKERGHTVVGVTMKL
                     WCYAEGPSPSRGCCTLDAIDDCRRVASKLGFPHYVLNLEEPFRQHVIEDFVGEYLAGR
                     TPNPCVQCNNWLKFGELLRAAERFDCEYVATGHYARRGVDPDGTATLLRSRDHAKDQS
                     YVLWGLAQDALRKALFPVGDLAKDEVRAVARRLELAVADKRESQDICFVEGRSYLDFL
                     KERFPKRLEASPRGEVRDQEGRALREHDGIHAFTIGQRRGIGVAANERRYVTKIDALT
                     GTVELGDERDLLRREAFLERVSYPSGVAPDRPFRVHGKIRYAHAPAPAEITPQADGRA
                     RVLFDEAQRAMTPGQSLVLYDGDRVVAGGVIERVP"
     gene            complement(18883..20043)
                     /locus_tag="VFR25_07690"
     CDS             complement(18883..20043)
                     /locus_tag="VFR25_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9940969.1"
                     /translation="MARVYLDHNATTQVRPDVLEAMLPYFSEHYGNASSVHAFGQEAR
                     AAVEDARAQVAAFLNASPAEIVFTAGGTEADNLAVIGGARSCASKGKHVVTTSIEHDA
                     VRQAADLLEHEGFQVTRVAPGPDGRVTAEAIEAALRPDTILVSVMASNNETGVIQPMA
                     EIGAVCAKRGIAFHTDAVQAAGKIPIDLAAWQATLATITAHKFYGPKGAGALFVRRGF
                     KCTPLQVGGEHERGRRAGTENVPAIVGLGKACEVAAAEMAAATPRITALRDRLEAGLA
                     ERVRQIIRHGASAPRVCNTAHVSFIGAEGEHLILSLDMKGIAVSSGAACKAGSSHPSH
                     VLLAMGVPANVAQSAVRFSLGRCTTDAEIDRVLDIVPGVVEKLRQGSPTLAS"
     gene            complement(20189..20998)
                     /locus_tag="VFR25_07695"
     CDS             complement(20189..20998)
                     /locus_tag="VFR25_07695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9940970.1"
                     /translation="MVSGFIRYTGVMLALGAFGAQPAFAAGAAKKSAASSDEVAVMET
                     TKGRMVIEFWDKEAPQTVDNFKKLARQGYFDGTGFHRIIKGFMIQGGDPKSKNPKAPD
                     LGTGDPGYKIKDEFNSHKHVAGVLSMANSGTPNSAGSQFFIMDGAAPFLDGKYTAFGH
                     VIEGMNVEKDIANTPVGPNAMMGGEKSKPLEWTTVKSVKIVPRSSLVAKAGGTPASAK
                     PAAATEVKSTPASAPAAEAAKAPEATAAAPAKTEQAPPPAASSEAKPDSAK"
     gene            complement(21032..22264)
                     /locus_tag="VFR25_07700"
     CDS             complement(21032..22264)
                     /locus_tag="VFR25_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940971.1"
                     /translation="MTPVRRPSHHPSHVLIALLLAAGCASHGSSKVAPATTAAAPVRP
                     ADHVAAGNDLMSRNLLDDAEHEYRLALEADPNDRGALAGLGQVNVLRGQYKEALPSLQ
                     RATQISSQMVGAFRSLGDAYTAMGDAQRASTAYRQAVALAPQDLRTRGALAQSLVAIG
                     ETGEAREICAATVRMTKGSPTDQARAYQQLGEVCAAETKIPESMSALYRASELLPGDP
                     EIARSLAETAVRGGLFAEAATAYTRVLTLAPLDVDAKKQLAFVNFKLERYPLAIKNYE
                     SVQDSLGTVDRYYLAQSYAKSNKVDRAVDLYREVAKADPQNYKGVYCNMAYAYYDASR
                     YQRAIEVAREGLAGDSASACLRFCWAQALDKLGRHEDAIPVFEAVLNDPAYAEPAKRE
                     LERQRRIVRLLKSKDRAD"
     gene            complement(22317..>23014)
                     /locus_tag="VFR25_07705"
     CDS             complement(22317..>23014)
                     /locus_tag="VFR25_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9940972.1"
                     /translation="FALGTAYRKAGKPQEALGAFQKMSALVPNEPGAHVSLGAVYMDL
                     KRPDDAYREFKKAIQLDPNDPSAHLNMGSYYVLQGKRDDALVSFKKATELKPPLAEAW
                     VNLGLIQAESGKIEDAVVSYNKALSIDSTNVHAICNLGNAKYGMGKIPEATELYKKAL
                     KLDASNQEALYNMGVAFADAQIYHEAINYWKKVVTVDSTTTVAESARGSIQVLQDFLD
                     QQKKSSAQTTGNQ"
BASE COUNT         3447 a         8060 c         7737 g         3770 t
ORIGIN      
        1 cgcccgaggc gcggcagcga tccgcgagga gcgcgagcag cgtcttccgc gagaggccgg
       61 agaatccgtg gcctgtcgag cggagcacca tccccgtgta gtggatctcg atgtcgtccc
      121 accggtggca gcgcgcgccc atctcggcga tgacctcggg gtcggcgtgt tccagcgccg
      181 cctcggtcgc gtcggagaag acgacgccga agccgaacgt gtcctcgagc cggtttcgct
      241 cgtagacctg aacctcgtgc ttgggctcgg ccttcttgag gaggagcgcg aagaagaggc
      301 ccgccggccc gccgccgatc acggccgcct tcacccggcc tccttgacga gctgccccgc
      361 gatcacgagg tgctggatct cggtggtccc ctcgtagatc cggagcgcgc gcaccgcgcg
      421 atagaggcga tccaccgggc tcgaggcgag cacccccgag cctcccagga tctgcacggc
      481 gtcatcgatg acgcgctgcg ccgcttcggt cgcgaacgcc ttggccatcg ccgcttccag
      541 ggtgacgcgc tcggcgccgc gatcggcctc gtacgcggcg cgatagacga ggaggcgcgc
      601 cgccgtgaga tcggtcgcca tccgcgcgag cttctcctgc acgagctgga attcggagag
      661 cggctttccg aactgccgtc gcgttcgcgc gtggcgcagt gcttcgtcga gcgcgcgcgc
      721 ggccatgccg caggccgcgg cgccgaccgt ggcgcgcaag cgatcgagcg tcttcaatcc
      781 gagcgcgagc ccacgccctt ccgggcccag gcgattcccc tcgggcacgc ggcatccctc
      841 gaacgcgatt tcacccaagg gatgcggcgc cgaaagcacc tgcgcgcgcg tgaacgtgag
      901 ccccggcgtc tccgccggaa cgacgaagca ggagatgccc ttcgctccga gcgagggatc
      961 cgtggtcgcg aagacggtgt agaagtcggc gatgcccgcg ttcgagatga gcgtcttcgt
     1021 cccgtcgagc acgtacgcgt cgccatcgcg ccgcgcgcgc gtcgcgatcg cagccgcatc
     1081 ggagccggcc tcgggctccg tcatcgcgaa cgctcccatg gcgcggccgg agacggcgtc
     1141 ctcgagccag cgcgatcgca tggcttcgtt ctcggcgaag tgaagcggga cggcggcgag
     1201 cccctggagc gcgaagacgg cgtccgcgag cggggacgcg gcgccgagcg cctcgcgcgt
     1261 gaggcagcag ccgcgccagt cctggcggcg gatcggatcg agcagccccc cctcgccgag
     1321 gcgcgagagc cacgcgcgcg cgcgcgtccg gccctccgcg tcgtccagag gctccggaag
     1381 ctcccgcgcc gcggccccgg cgaatgctcc cacgaccggg gcgagcgcct ggtgcgcgtc
     1441 gtcgaggaac gcgcgaaccg ggatcggatc gggatggctc atgcctggaa cttgggctcc
     1501 cgcttggccc ggaacgcctc gtaggcctcc cggaaatcag gacgctccat gagcgcggcc
     1561 tgggcgtccg cctcgtgcgc gagcgccgat tcgaagtcga gcgcccactc ggcgtcgagc
     1621 gcgcgcttcg tgaccgcgag cgcctcggcg ggtccgcgcg cgagacgcgc ggcgagcgcc
     1681 acggcctcgt ccacgacgcg gtccacggga acgaccgcgt tgtagaggcc gatctcctgc
     1741 gcgcggcggg cgtccacgaa gtcgccggtc atgaggatct ccgtcgcccg tccgtgcccc
     1801 acgatccgcg gcaggagcca cgacgcgccc atgtccgccc ccgagagccc gaccctcacg
     1861 aagaggaatg cgatcttcgc ggtctccgag gcgatgcgga cgtcgcacgc ggcggcgatc
     1921 accgctcccg cgccggccac cgtgccgttg agcgccgcga tcacgggccg gcggcagcgc
     1981 cggatgttga ggatgagatc gcaggtcatg cgcgtgaact cgcgcagccc tgccgaatcg
     2041 cgggagaaga gagcgccgat gatgtcctcg acgtcgcccc ccgagcagaa cgcgcgcccc
     2101 gatcccgtga ggacgatcgc gccgacgccg ggctccgtgt cgaggcgggc gaagagatcg
     2161 cgcagctcgg cgtagacctc gaatgtgagc gcgttcagcc gatcgggccg gtcgagggtg
     2221 atgcgcgcca cgcgggtcgc cggatcgacc gcgaatcgga aggagcgcgg ctggatcacg
     2281 gcgtcccccc gatgacggcg gtagcctcga tctcgaccac cgcgccctcg tccacgaggc
     2341 ccgagacctc gaccatcgcc atcgccggat agcggcggtc gaaccgctcg cgccagacgc
     2401 gccccaaggg ctcgagcgcc acgcggtagg agttgagatc cgtcacgtag accgtcatgc
     2461 gcgccacgtc cgccggtccg ccccccgcct cgcgcaccac ggcgagcacg cggtcgagcg
     2521 cgcgcgcgaa ctgctgcacg aatcccgcga cgggcccctt ctccgacgcg tcccaccccg
     2581 gctgcccggc gacgaagagc acgcgccctc ccgcgggccc gagcacgccg tggctgtacc
     2641 ccttgggcgt gcccagcgac gcagggttca cgttctcgag gctcatcgac ccctcccgtt
     2701 caagacgatc gcctgtcccg tctccaccgc gtcgtcgcga cagagtcgca ccacggccgc
     2761 cgcgacttct tccggacgga tgagccgctc ctgccccgcg gtctcgagca cggctcgaag
     2821 cgcggcgtcg cgggcgagcc ccgtcttcgc tccgaccgtg gcgagcgtcc gatcggtcat
     2881 gggcgtgtcc acgtaggccg gacagatcgc gttcaccgtc accccgcgat cctcgagctc
     2941 gagcgcggcc gcgcgcgtga acccgacggc ggcgtgcttg gaggcgcagt agtgcgcgat
     3001 gtagcgcccc ccttcgagcc ccgaggtcga cgcgatcgtg atcacgcgtc cccatccccg
     3061 ctcggccatc gcgggagcga actcccgcgt gcagaggaag acgccggtcg cgttgaccgc
     3121 gaggacgcga ttccactcgt ccagcgtgag cttccggagc ggcgccgagc tcgagacgcc
     3181 cgcgttgttg acgaggatgt cgaccttgcc gagcttggcc tccgcgtcgc gcgcgagcgc
     3241 cttcacgctc gcctcgtccg tgacatcgca cggggcggcg tgcgcctcta cgccgcgcgc
     3301 gcggagatcc gccgcgaccg catcgacctc ctcccgcgtg cgcgccgcga ggaggagcgc
     3361 gatcccctca tcggcgagcg cgtgcgcgat ggccgcgccg atgccccgcc ccgcacccgt
     3421 gacgaccgcg gtccggccac gcttcatatc gtggcgtgct ccgatccgaa cgcctccaga
     3481 accgcgtcgc agaggatctg cccgagcgcc tcgatggtct cgcgcccttc ggcgtcggtc
     3541 gcgtcggcgg gccacccgaa gtaggcccgc ggcccccccg cctcctcgaa tgtccccttg
     3601 ccgtcccgaa tcgcacggga tagcgacgcg ggattggggg gaagggaccg catgacctcg
     3661 cggcgcacga ggtccggccg ctcggccatc acgatcgatc cttcgtaccg ccccgcatgg
     3721 caggcgccgg tgcggaactc gtccgtgagc cggagcgccc agggctttcg cgtgagtaga
     3781 ggacagacga tgggaatgga tccatcgcgc cgcgcgcggt ccactgcttc ctggagcgct
     3841 cctacatgag cgggatcgag atgcgcattc gcgaccgcca gcgccgccac gccatgacgc
     3901 ttgagctccc gcgcgagcgc ggcgacgagc cccgtgacga tgtcggccgg catcgaaacg
     3961 gtgcccgcga acgcggcacc gaatggagcg gccgtgtacg ggagcgccgg taggagcacg
     4021 gcgtggatcc ctgccccggc gaggcagtcg ccccccgccc gcgccatcgc ctcggcgatc
     4081 accaaatcgg tgccgagcgg cagatgcgga ccatgggcct ccacggcgcc gaccggcagg
     4141 atcgccgtca gccgctcgcg cgggaggtcg cgcacctctt cccaggtcag atccgcccag
     4201 cggggaaccg tcattcaatc ctcatgtcgg gtattgccac ccgccgtgcg ggccctcatc
     4261 atgggcggct cggacgaacc taccatgacc cttgagccat cggaacgact gaacatcgcc
     4321 gatctctttc tggacgagcg ggttcgggaa gggcgaggcg cccgtaccgc gatccgcacc
     4381 gactccggaa cgagcacgta tgccgaggtc caggcgctcg ccaaccgctt cgcgcaggcg
     4441 ctgcggcagg ctggagtgca gcccgagcat cgcgtgatcc tcggcatgcc cgacggcgta
     4501 ggctacgtcg ccgcgctgtt cggcatcttg aagctgggcg gggtcgccgt gatggtgaat
     4561 ccgggcctgc ccgccgccga agtcgcgtgg atcctggatc acgcgcgcgc ccgggccatc
     4621 gtgacgcacg cgagctcggc cgagccgttc ctccagtcgg ccgcagtgtc gccgttcgcg
     4681 cccaaggtgc tggtccatgg cgacgacgcg ttcgaccaca cgctcgaggc ccagtcggac
     4741 acgttcccca ccttccccac gcaccgggac gatcccgcga tctggctctt ctcgggggga
     4801 acgacggggc ggccgaaggc cgtcgtccag acccatcggt cgttcgcgaa caccgcgcgt
     4861 ctctacggcg gccaggtgat cgcctacacg gaggacgaca tcacgctctc cgtccccaag
     4921 ctctacttcg gctacgcgac cggctcgaac ctcttcttcc cgttcgcggc gggcgcatcc
     4981 accgcgctct tcccggagcc cgcgaacgcg gccaccttgt tcgacaagat caagcgccac
     5041 aagccgacga tcctcgtcca ggtcccgacg atgatccaca agatgctctc gcatccgggc
     5101 gccgcggctt cggatctctc ctcgctccgg ctcgtgacct cggccggtga agctctcccg
     5161 gcggagatcg acgagcgctg gcgccgcacg tttggagtcg agatcctcga cggcctcggc
     5221 acggcggaga tgtggcacgt cttcatctcg aaccggccgg gccaatcccg gcccggcacg
     5281 ctcggcacgg tcgtgccggg cttcgaagtt cgcgtctgcg acgaggaggg gcgcgagctc
     5341 ccgcgaggcg agaccggctg gctctgggtg aagggggact cgcgcgcgct cggctactgg
     5401 cacgacctcg acaagaccgc ccggagcttt cgcggcgagt ggtacgtctc cggcgatctc
     5461 atcacgatgg acgcggaggg ctacgtgacc tactgcggcc gcggggacga gatgctcaag
     5521 gtcgcgggca agtggcttgc gcctcaggag gtggaaggct gcctcctcga gcatccatcg
     5581 gtggcggaag cggtggttgt gggcgttccc gacgcgcaag gtctcgtgaa accgcgcgcg
     5641 tacgtcgtgc ccaagggcgc gaaggacggc ctcgaggaga cgctcaaggc gcacgtgaga
     5701 gatcgcctcg acgcgtacaa gcatccgcgc gaggtcgtgt tcgtcgactc cctccctcgc
     5761 acccacctgg gcaaggtgga ccggggatcg ctcaggcggc gctgatggcc accccgatct
     5821 cgatcgtgcc gcgcaagagg ccggcgcccc ctgcggccgc ggcggtcccc agtaccgaca
     5881 ggcagcgact tcccggcacg cggctccagg tcgcgatcct tgcgttcgcg gtgttcgtcg
     5941 tggccgcaat cgtgcattcc cagaccctct ccttcaggtt cctgacgacc tgggacgacc
     6001 cgtcctacat caccaacaat ccctggatcc ggggactcac gctcgagaac atccgcttcg
     6061 cgttcaccac gccgtacttc gcgaactacc ttccgctcca cctggtctcc tacatggtgg
     6121 actaccagct ctggggactg aacccgttcg ggtatcgcct gcagtcgctc ttcctcgtgg
     6181 cgctgaacgc gtcgttggcg ttcttcctcg tgcggcggct cttccggagt ctggcgctcg
     6241 gggtcattgc ggcgctcctc tacgcggtcc acccggccca tgtggaagcc atcgcgtgga
     6301 tctcgatccg aaaggatctc ctctcgaccg tgttcctcct cttcacggtc atcctgtacg
     6361 acgaggcgac tcgcgaccgc ttccgcccgt gggtgtacgc gatctcggtg gtgatgtacg
     6421 tcctcgggct cctgtccaag gtgtccatct cgacgcttcc cctctttctc ttcgtgctcg
     6481 actggacgcg gcggtgggat gggcgcgcgt tccgatggaa gcagtcgatc gccaccaaga
     6541 ttccctacct cgccttcgcc gccgtgctcg tttatgtgaa caatcacgcc caggtgaaga
     6601 ccggcatgcc ttacacgcac cagccgctcc agttcatcat ggtgaagggg gaagcgatct
     6661 ggagatacct ggcgatgctg acgggaatgg tcgacggagt cccgatgtac gacccgcctt
     6721 ggctgggcgg cctgcacccg cccttcaatc tggccggcgc cctcctcccg ttcgtcgtcc
     6781 tcggcttcgc gttctggcgg cgactccggg ccctgacgct cggcgcggcc tggctcttca
     6841 ttctcctcat cccggttctc gcgtttccgc tcatggtgta catggcggac cgctacctct
     6901 actccccctc gctcggcttc tgctgggtcc tggctgcggg catcctgtgg ctggccggcc
     6961 gggtgcgctc tcagcgcggg cgcgccgcgg cggcggtcgt gctgaccgcg atcccgttca
     7021 cgctcttcac catgaggacg gtccgttacg aggcgttgtg ggggaatccc gaggcgctct
     7081 ggagatacac gatgcagcac accaaggacg tgcgggccaa gagcgctctc gcgcagaccc
     7141 tgacgctgca gcggcgctac gacgaggcgg agcagatcta tcagagtctc atggtctaca
     7201 agagcaccga cgtctatgcc ggactcgcgt ccatctacat acccacggga cgattcgacg
     7261 aagcgcagga gatgatggac caggccgtac tccattgggc gggcaagcgc gacaacgaga
     7321 aggccagcat cctctgttac cgcgccatgg tcgaatgggc ccgcgaggac aaggacgcgg
     7381 cgattcgcga ttggatcgag gcggcacgcc tcgacccgaa gaacgacgag tcgcggatcc
     7441 acttgcgccg ccttggcgtc gagccgccgg catcgcgctg accatggccg agccgatcac
     7501 gatcgtcccg cgccggaagc gagccgagcc ggtggcaggc tccgccgcga gtcgccccgt
     7561 caagcgggac ggcggtctcc agttctggat cctcgccgcc acgtcgttcg gggccgcctt
     7621 gctggtccac gctcagacgc tcgggttccc attcttgatg acgtgggacg atccaaccta
     7681 cgtcgtccac aatccatgga tccgcgggct gactctggag aatttgcgct tcgcgttcac
     7741 agcgccctat ttctcgaact acctcccgct ccacctcgtc tcctacatgg tggactactc
     7801 ggtctgggga ctgaacccat taggcttccg gatccagtcg ctcctgctcg ccgggatgaa
     7861 cacggctctc gccttcgcgc tcacgcgcag gctgttcggc agcgtcgctc tgggaatcgt
     7921 ggcggcgctc ctctatgccg tccatccgtc ccacgtcgag gcggtcgctt ggctctccat
     7981 ccgcaaggat ctgctctcga ccttctttct gctgctctcg gtgctcttct acgacaaggc
     8041 gactcgagag ggattccatc gagcgtggta cgcgacgtcg gtcgcgacat tcctgctcgg
     8101 acttctgtcc aaggtttcga tctcgatgct ccctctgttc ctcctcgtgc tcgacctcac
     8161 gcgccggtgg gacggaggcg cgttctcgtg gaggcgctcg atcgcgacca agattcccta
     8221 cgcgttcttc gccggggcgc ttgtcgtggt gaacgacctg gtccaggtca ggacgcacct
     8281 gccgtacgcg catcagcccc tctcgtattt gatggtgaag ggacattcgg tcgcgatgta
     8341 cctggggctc ctggcggggg ttttcgacgg gaggccgatc tacgacccac cctcggtggc
     8401 cgggtttcga ggggtcctgg atctggccgc cctcgtcttc cccctcgtgg tgttcgcgtt
     8461 tgcctggtcc cggcgatccc gggtgctcgc gctcggcacg gcctggatct tcgtcctcct
     8521 gctccccgcg atcgccttcc cgctggtcac gtacatggcg gaccgctatc tctacgcgcc
     8581 atcgctcggc ttttgctgga tcctggcggc aggcatcctg tccctggccg gcaaggcacg
     8641 atccgagcgc cggcgcgtcc tggcggtcgt cgccatgacg gcgctggcgc tcgcgttttt
     8701 cgcggtgcgg acggtgcgat acgaatcggt ttggggtgtg ccccagaagc tctgggccta
     8761 cgcccatcag cggagtcatg acgtccgggc gacgagcaac ctggcgcagg cgatgtacgt
     8821 gaaccacaac tacgacgagg cggagcagct ctaccgcacc atcctaggga cgaacaccgt
     8881 cgaggcgtgg acgggtatcg cgtgcgtcta cttcaacacc cggcgctact ccgacgccaa
     8941 ggacgcgatg gaccacgcgg tcctcgccat gggcagaagg cggccgagcc agcaggcgga
     9001 cgtcctctat cttcggggcc agatcgagtg gcccttcgac cgggaggcag cgatccgcga
     9061 ttgggaggag gcggtccgca tagacccgaa gcacgagcgg gccaaggcca gtctcagcgc
     9121 gcctgacgcg cctccgagcg ggctctgatc atggcggcca tctcgagatc ggcgcgccgg
     9181 gagcgcgcga agcgcgatgc gaagatgggg cgccccgcag gcggcgcgcc gggagcgcgg
     9241 ctccccggca cgcttcttca gctcgccctc gcttcgatcg cggcgttcgc cgccgcgtac
     9301 ttcgtccatc agccttcgct tcacttcggc tttctgacga gctgggacga tcccacgtac
     9361 gtcacggaca atccctggat tcgcgggctg acagccgaga acatcaagtt cgcgttcacg
     9421 accccatact tctcgaacta cctcccgctc cacctcgtct cgtacatggt ggattactcg
     9481 ctctgggggc tgaacccgtt cgggttccat ctccagtccg tgatcctggc ggcgctcaac
     9541 gcggtgctcg cgttcttcgt cgtgcgccgc ctcttcggca gcttcgcgct cggatgggtc
     9601 gcggcgctcc tctacgcggt ccacccgtcc cacgtggaag cggtggcttg ggtctcgatc
     9661 cggaaggacc tcctttcgac cgtctttctc ctcgtcaccc tgatcctgta cgacgaggcg
     9721 acgcagggcc ggaagctccg ggtggcgtgg tacgccgcat cggtcgtgac cttcttgctc
     9781 ggactctggt cgaagatgtc cattgtcgcg ctcccgctct tcatgctcgt gctcgacttc
     9841 atgcgcgacc ggcagggacg cccgttcgac tggaagcggg cgatcgtgac caagatcccc
     9901 tacggcctcc tcggggtctg gctcgtgatc gtgaacaacc aggtgcaggt gaagtcggaa
     9961 cagccctacg cccaccagcc gctccagtac ctcatggtga aggggcacgc ggtctggctg
    10021 tacctcggcc ttctcacggg catcacccag ggacggccga tctacgaccc cccgtccttg
    10081 agtgtgctcg atctggtcgg ggtcctgatc ctgcccgcga tcttctggct cgcgtatcgc
    10141 cgccggcttc gcgcgctcgc actcggcgcg gcatgggtct tcgtgctcct gatccccgct
    10201 ctcgcgttcc ccctcatcac gtacatggcc gaccggtacc tctatgctcc gtccctcggc
    10261 ttctgctgga tcctgggcgc cggcctcgtc gcgatcgcga gccgcgtcaa ggcggagccg
    10321 ggacggacgg tgacgctcgc cgcgatcacc gcgattccgt tcgtattctt cacggtgcga
    10381 acacgggagt accagagcgt gtggagggac ggtgaagcgc tctggaccta cacgaccaag
    10441 cacagccgcg actttcgcgc gctcaacaat ctcgcgcagg tccggttgaa tcagcaacgc
    10501 tacgccgacg cggagcgcct gtatggacag gcctcgaagt tcgacaacat cgtctcgtgg
    10561 caaggactcg cgaccacgta ctacaacacg aagcgctacg agcaagcgca ggcggcgatc
    10621 gacaaggccg tcgcgatcat gaagacaaag cgttcgagcc ccgatgagca agcggagctc
    10681 tactacacga aaggcgccat cgaatgggtc cgcggccaga acgccgccgc gcaacaggac
    10741 tgggaagaag ccgtccgcat ctacccgcaa catgcgcagg ccaacgcgtg gctccgcacc
    10801 gcccgcggcc aggctcctcc accgcctccg cctacgacga cgcactgatc cacgctgcag
    10861 atcgatacgg gagcaacctc cgggcgcggc cattgcttcg cacttcgcgc gaagctcgcg
    10921 aggcgccctc cggttgtccc ggaccctccc cctagcttgt cgaggtgaag cctagatcag
    10981 ccgcggacgt cggggcggac tccggggggg aacgcgagcg cgaagaacgg agggatgacg
    11041 ccgacgaggc ggagatacag cgcgagctgg gccttgtggt ggacctcgtg ctccaccagg
    11101 taccagagcc aggcgcgcag cgtccagtcg acggggacgt cggggacctg aacgttgtcc
    11161 tcgaggcgag ccgggtcgag actgtcgagc gccttgagtg tcaccgcgcg cgtttcgtgg
    11221 agaatggcac gcagggattc cttagtcagg ctgtccgggc gcgtgaagta gcgctcgcgg
    11281 ttccagtcgc cgaggaggag gccgcggacg tggtactcct cggtgcgcgc gatgtggagg
    11341 aattgctcgt cgaaggggcg gagcccttcc tgcggagcga agtggaggtt tccctccggc
    11401 acccactcga gcgtctgcag cgtgacccca cgaaaccgct cgaggtgctc ccggatgtcg
    11461 gctacggcgc gcgtctcggt cttggccatg tcgcgtgtcc ccctttggcc tggaaggtta
    11521 gcaccccgct tgtctgccgc ccaggggccg cgtaccattc tcggccatgc ccgtcgaaac
    11581 cgaggaagag ctcgccgcga tcgtcaagag cgccagcgtc gtggccgtga tcgggatcaa
    11641 ggacggctcc gacccgcacg cccctgccta ctccattccc aagatgctcc aggagcgggg
    11701 aatccgcatc gtccccgtcc accccacgct cacgagcgtc ctcggcgagc gggtctaccc
    11761 ggcgatcggc gacgtgcccg agcgcgtgga catcgtggac ctcttccgga ggcccagcgc
    11821 gatcgacgcg cacgcggacg agatcctcgc gcttcccgag tccaagcgcc ctggcgtggt
    11881 ctggatgcag accggcatcc ggaacgacgc ggccgcggcg cggctcgaag ccgcgggaat
    11941 ccaggtggtc atggaccgct gcctcggcgt ctacagcgcg cgctaccgcc cgcgggcctg
    12001 acccgccgcc atgtcgacat tggccgcaag gatcggatag cgccccctct cacgcgcgct
    12061 taatctttcc tccatgaacg cgatcgacga acggcgctgg caggcggtgc tcgctcggga
    12121 cccgagccgg gacgggacct tcgtcacggc ggtcctgacg accgggatct actgtcgtcc
    12181 ctcgtgcccc gcgcgccacc cgcgccccga gaacgttcgc ttctatccca ggccggacga
    12241 cgccgagaag gcgggcttcc ggccctgcaa gcggtgccgc ccccgcgagg agcggcgccc
    12301 ggatcgcgca accgatctcg tgccgcgcgt ctgccgctat ctcgacgaga accgggacga
    12361 gcgcgtcacg ctcctcgacc tcgcgcgcga gatgaagatg agcccgcacc atctccagcg
    12421 caccttcaag cgcgcgacgg gcgtgtcccc tcgccagtac gccgaggcgc tccgtctcgg
    12481 tcgtctcaag cagcgactcc agggaaagga accggtcacc atggcgctct acgaagcggg
    12541 ctacggatcg agcagcaggc tctatgaacg cagtaacgcc cggctcggca tgacacccgg
    12601 cgcctaccgc gcggggggca agggctccgt catccgatac gcgatcgcga gcactccgat
    12661 cgggcgcgtg ctcgtggcgg ccacggagaa aggggtgtgc agcgtccgaa tcggagagcc
    12721 cgcggagacg atggagcgcg ccttgaagcg cgagttcccc gaggcgacgc tccgccgcga
    12781 tcgggccgga ctcgccgcct ggatcgagcg tatcgcgcgg catctcggcg ggcgtgcgcc
    12841 gcacctggat ctaccgctcg acgtgcgcgg gacggcgttc cagtggaagg tgtgggaggc
    12901 gctccaggcg attccctacg gggagacgaa gacgtatcgc gaggtggctc gcgcgatcgg
    12961 caagccgcgc gcggcgcggg cagtcgggcg cgcctgcgcc acgaacccgg tggccatcct
    13021 catcccctgc caccgcgtcg tgcgggaagg gggcgccctc ggcgggtatg cctacggcct
    13081 tcccaccaag aagcgcattc tcgacaccga gcgctcgagc gcgcccgcga gcgcccggag
    13141 gcgccgtcag accggcgcgg ggaggatgcc ccggccgatc gcgtagcgca cgagctccgt
    13201 ctggccgcgg atgttcagct tccgaagcga gttcgcacgg tgcgtctcgg ccgtccgcgg
    13261 gctgatcgaa agccggtccg cgatctgagc gctggtcagc ccctcggctg cgagatggag
    13321 cacctcgcgc tcgcgcatcg tgagcgtctc gtaggcgtcc gccaggtcgc gatccgccac
    13381 gccgctctcc tcgagaggcc ggctcaggta gcgacgccct tccgctacct cgcggattgc
    13441 gtggaagagc tcgtcggcgg aggcatcctt gaggacgtac cccgccgcgc cgttcttcat
    13501 ggatccgctc acgtaggcgt cgttcgcgtg catggacagc atgagcacgc gcgttcgggg
    13561 agttcgtttg gccacttgct tcacgacgtc ggcgcctccc agccccggca tcgccacgtc
    13621 gaggagaagc acgctgggct tggcattctc gacgacgttc actgcttcga gtccgtcgcc
    13681 ggcttgcccg acgacctgga aggacgcgcc ctcgagcagg accttgagcg cctcgcgcat
    13741 gatccggtgg tcgtcggcga ggacgaccgt gatgggttcg ttcatgattc gagcctctgg
    13801 gttgaacgga acggtcgcgg tgaccgaggt cccgcgtccg cgcctggatt ggatgacgag
    13861 ctcccctcgt aacgggcgga gcctctcctt catgccggcg atgccggtgg atccggacgg
    13921 gataccctcg gaatcgaagc ccgtgccgta atcccggacc cggaccccga tccatccccg
    13981 tcctgtacga agcaccacgc gtgcgcgcga cgcccgcgcg tgccgcacgg cgttcgtgag
    14041 cgcctcctgc acgacgcgat agagcgcgat ctcgaggtcc ggatgatacc ggccacagac
    14101 tccaaaccga cggaagtcga cccggattcc ggtctgctcg ctgaactgcc tcgcgtgcca
    14161 ctcgagtgtg ggcgcgaggc cgaagtcgtc gagcaccgac ggccggaggc gctgcgcgat
    14221 attccggacc tgctgcatca gtgaggcaag cgccgtctgc accaactccg atcgagtccc
    14281 cgaggcttca aacgagaatt tgagcgccgc gatcacctgt cccacttcgt cgtggagatc
    14341 gcgcgagatc gcgcgccgct cgctctcgtg cgccgtgacc agcttgcggg agagcatctc
    14401 cagctcctgc cggtcgcgcc atgcctcctc gatgacccga gcgcggctca tccggggggc
    14461 aatccggtcg gccgtgatgc ggacgagtcg gaccatgtgg ggcgtgacct gcttggggtg
    14521 gtggtggaag aggaccaggg caccgatccg ttcctcttcc acgaccagag gagccacgaa
    14581 gatggcctgc agcgttttct ggtcgtttcc atggaggcgc tccagggcga tcggggactt
    14641 cccgagcgtc atcgacccaa cgatctcgga gcagtcgccg gggatcggga ggacgttccg
    14701 ggacgagctc agggcgagct gctccggaac gaccgagccc cgccgggtac cgaaggcggc
    14761 gacggcgtcg acgtgcagcc cggaccgcag cgcctcgacg cagccctgga ccatcttgat
    14821 cgtcggcagc tcggcgtgga cgctggccat gatcgacttc atagcatcga gctcgccctt
    14881 gaccacttca gcccggccgc gctcggattc cttcccggcc tcgagctgct cgcactcggt
    14941 ctcggcggtc ttcaaggcaa gggcggtgcc gacgtgggct gccagcgcct cggccacgag
    15001 ggggatgggg tcgcccggcg acatgggctc ggatcggtag aattcgagca cggcaaaggc
    15061 ccggtcgcgc accttgatcg ggatcgcgaa tcccgcttcg agtccggcct gaagggcctc
    15121 gttgcgccgc gtctcgtcga ggaatcgatc cggcggattg aggacccgga tctcgccgtc
    15181 ggcgagcgcg cgggccggta gggagccagg gcccgccgag cccagacaac ggctcttctg
    15241 gacgaatcgc tccacatccg aatcaccgga gtgccaggcg gaacaccaca ccgggtccga
    15301 atctggagtg gcccggtagg cttgcccgac ccggaatccg tccagatggg cgatgcagcg
    15361 gacgagcagg cgaaggagct cggcagcatc gctctcttgc gcgatggcca tcgcggcctc
    15421 gcggagaacg ttcgcctgtc tcccccagtc tacgttgggt tcggccgtgc tggagacgtg
    15481 cacgggtcac ctcggatcac gttgggacgc tcataccggg acggcccccc ccggcgcctg
    15541 agacccgaac gcatcgagtc ccagtgattt atggaaaccc acgcggcccc gacccgcaat
    15601 acgtatcgat acgcaaaccg gccggacggt caaactgcgt gggaccgagg ggcttaggag
    15661 gcggcgggac ctgcggtttg aagcgcgaca gccagcccat gcgctgtcga gcacggcgct
    15721 aagcaatgat acccctcgca gagatacgct gtcccggatt tttcgatcgg agggaacgag
    15781 gccagagccg ggacccatcg cctcaggtcg ctgatcgtct ccggcgccgc acgcaagagc
    15841 acggcgcggg gaagaaagcg ccgccccacc tcgcgaacca tggcctcggt ctccgcatcg
    15901 cgcgccgctc ccgcgatcac gatctcctgc gcggggccga gcgccagatc gaccgcaccg
    15961 aggagacacg catgtccggt gggatgggat gaggccgagc ccgcgaacgc gtccagtacg
    16021 gcgcgccccc gggtctcacc ccacggatcg cccgtcattc gagacagccg gagcagcgcc
    16081 gaggccgcga ccgaattgcc ggatgggatc gcgccgtcgt aggactcctt cgtgcgcgcg
    16141 aggagcggct ccgcgtcctg ccccgtgaag aagtagccgc cctcggccgt atcccagaag
    16201 aggcggtcca tctcgcgcgc gagcgcgacc gcttcctcga ggtgagcggg gtcggaggtg
    16261 gcctcgaaga gctcgaggcg tgccctcagg acgaacgcgt agtcgtcgag atagcccgcg
    16321 tgcgccgcct cgccgtcgcg gtagcgcgcg agcaggcggc cgtccccacg cacgaggcgc
    16381 gccttgacga actcgagggc gcgcccggcc atcgctgcgt acgcggggcg gtcgagggcg
    16441 cgcgcggcct tggcgaggga tgcggccatg agtccgttcc aggccgtgag gatcttgtcg
    16501 tcgcgatgcg gtcgcggccg gcgttcgcgc gcctcctcga gtgccgcgcg gcagccgtcg
    16561 agctcgagcg cgagtgccgc cggatcggac gcgagctcct cggcgagcgc cgcgggatcg
    16621 cgatcgagat agggaatgtt cgttccgggg cgcgtcccgt gcgcctcgtc cacccagttc
    16681 ccgtccttct cgatgccgta ggcgcgcgcg tagagcgcgc cgcgctccgc tccgagcacg
    16741 cgatcgatct cgtcgcgcgt ccagacgtag aacttccctt cctccccctc gctgtccgcg
    16801 tcctccgccg aatagaacgc gccctccggg gacatgagac ggcggccgag atacgtgacg
    16861 atctctccgg ccacgccggc gtagagcgac tgtccggtcg cctgatacgc ctcgacgtac
    16921 gcgtcgagga gaagcgcctg gtcgtagagc atcttctcga agtgcggcac gcgccactcg
    16981 cggtcggtgg cataccggtg gaagccgaag cccacctggt cgtagatccc gccgcggcgc
    17041 atcgcctgga gggtcccttc gaccatcgcc agcgcacgac catcctgggt gcgattccag
    17101 taccggagca ggaacgtgag ctggtggggc gtcggaaact tgggagcggt tccaaacccg
    17161 ccctgctcgg cgtcgtagcg cgctgccagg tcctcgaacg cggcgcgcat cgtcttctcg
    17221 tcgagccgct cccccggatg cgccgcgaag ttgggacgga tcgcgtcgac gatctgggtc
    17281 gccacggtga ggatctggtc ccgctggctc tcccacgcgc cgacgatctg gtagaggagc
    17341 tcccggaggc ccgggcgtcc gaaccgcgtc tccggcggga agtaggtgcc ggcgaagaag
    17401 ggctcccgcg cgggcgtgag gaagacggtg agcggccagc cgccgcttcc cgtgagcgcc
    17461 tggcagacgg tcatgtagat gtggtcgacg tcgggacgct cctcgcggtc caccttgatg
    17521 gccacgaagt gctcgttcag gatgcgcccg atctcctccg tctcgaagga ttcgcgctcc
    17581 atcacgtggc accagtggca cgtggagtac ccgatcgaga ggaagatcgg cttctcctcg
    17641 cctgcggcct tcgcgaacgc ctcctcgccc caggggtacc agtcgaccgg gttccccgcg
    17701 tgctggagga ggtagggact cttctcgcgg gcgagccggt tccgcgcggg cgcgctcacg
    17761 gcacgcgctc gatgacgcct cccgcgacga cgcggtcccc gtcgtagagg acgagcgact
    17821 gtcccggcgt catcgcgcgc tgcgcctcgt cgaacagcac gcgcgctcgc ccgtccgcct
    17881 gcggcgtgat ctcggccgga gcgggcgcgt gcgcgtagcg gatcttcccg tgcacgcgga
    17941 aggggcggtc cggcgccacg cccgagggat agctgacgcg ctcgaggaag gcttcccggc
    18001 ggaggagatc gcgctcgtcg ccgagctcga ccgtcccggt caacgcgtcg atcttggtca
    18061 cgtagcgccg ctcgttcgcg gcgacgccga tcccccggcg ctggccgatc gtgaacgcgt
    18121 ggatgccgtc gtgctcccga agcgcgcgcc cctcctggtc tcgcacctcc ccgcgcggcg
    18181 atgcctcgag ccgcttgggg aagcgctcct tcaggaagtc gaggtacgag cgcccctcca
    18241 cgaagcagat gtcctggctc tcccgcttgt ccgcgaccgc gagctcgagg cgccgcgcga
    18301 ccgcgcgcac ctcgtccttc gcgagatcgc cgaccgggaa gagcgccttc cggagcgcgt
    18361 cctgcgcgag cccccagagg acgtacgact gatccttcgc gtggtcgcgc gagcgcagga
    18421 gcgtcgccgt gccgtccgga tccacgccgc ggcgcgcgta gtggccggtt gccacgtact
    18481 cgcagtcgaa gcgctcggcc gcgcggagga gctccccgaa cttgagccag ttgttgcact
    18541 ggacgcaggg gttgggcgtg cgtcccgcga ggtattcccc cacgaaatcc tcgatcacgt
    18601 gctgccggaa cggctcctcg agattgagga cgtagtgcgg gaatccgagc ttggacgcca
    18661 cgcggcggca atcatcgatg gcatcgagcg tgcagcagcc gcgcgagggc gagggtccct
    18721 cggcatagca ccagagcttc atcgtgacgc cgacgaccgt gtgcccgcgc tccttgagga
    18781 gcgccgcggc gaccgcggag tcgacgccgc cgctcatggc gacgaggacg gtcgtagggt
    18841 ggtcgggaag gaacgagtcg ttcaacgagg cctccgggtg cttcaggagg cgagggtcgg
    18901 ggagccttgg cggagcttct cgacgacgcc gggaacgatg tcgaggacgc ggtcgatctc
    18961 ggcgtcggtc gtacatcggc cgaggctgaa ccgcaccgcg gattgcgcga cgttggccgg
    19021 cacccccatc gcgaggagga cgtgcgacgg atgggacgat ccggccttgc acgcggcccc
    19081 gctcgagacc gcgatgccct tcatgtcgag gctcaggatg aggtgctccc cctccgcgcc
    19141 gatgaacgag acgtgggcgg tgttgcagac gcgcggcgcc gaggcgccgt ggcggatgat
    19201 ctgccggacg cgctcggcca gtccggcctc gagccgatcg cgaagcgcgg tgatccgcgg
    19261 agtcgcggcg gccatctccg cggcggcgac ctcgcacgcc ttgccgagcc ccacgatcgc
    19321 cgggacgttc tcggtgccgg cgcgccggcc gcgctcgtgc tcgcccccga cttggagcgg
    19381 cgtgcacttg aacccgcggc gcacgaaaag cgcgccagcc cccttgggac cgtagaactt
    19441 gtgggcggtg atggtggcga gcgtcgcctg ccaggcggcg agatcgatcg ggatcttccc
    19501 cgccgcctgc accgcgtccg tgtggaacgc gatgccgcgc ttcgcgcaga ccgctccgat
    19561 ctcggccatc ggctggatca cgcccgtctc gttgttcgac gccatgacgg agacgaggat
    19621 cgtgtcgggg cggagcgccg cctcgatcgc ctcggccgtg acgcggccgt cgggaccggg
    19681 agccaccctc gtcacctgga atccctcgtg ctcgaggaga tccgccgcct ggcggaccgc
    19741 gtcgtgctcg atggaggtgg tgacgacgtg cttccccttc gacgcacacg agcgggcgcc
    19801 gccgatcacc gccaggttgt ccgcctcggt gccgccggcc gtgaacacga tctccgccgg
    19861 gctcgcgttc aggaacgcgg ccacctgggc ccgggcgtcc tccaccgcag cgcgggcctc
    19921 ctggccgaag gcgtggacgc tggaggcgtt gccgtagtgc tcgctgaaat aggggagcat
    19981 cgcctcgagg acgtcggggc gtacctgggt ggtggcgttg tggtcgaggt agacgcgcgc
    20041 catcgatgtc ctcatgaagt cggaaaggtg gtgcgggagg ccacgttcag cccttcaaga
    20101 gtagcaaaac gaagggggcg gacgcaacgg cgcccgcccc ccgaatcacg ctcgcggccg
    20161 ctccaaggag cgccgcatct cgcttcgatt acttcgccga gtcgggcttg gcctcgctcg
    20221 acgccgcggg aggcggcgcc tgctcggtct tcgccggagc cgcagcggtc gcctcgggcg
    20281 ccttggcggc ctctgccgcc ggagcggacg ccggagtgct cttcacctcc gtggccgcgg
    20341 ccggcttggc gctcgccggc gtgccgcccg ccttcgcgac gagcgagctc cgcggcacga
    20401 tcttcaccga cttcacggtg gtccactcga gcggcttcga cttctcgccg cccatcatcg
    20461 cgttcggacc gaccggcgtg ttggcgatgt ccttctcgac gttcatcccc tcgatgacat
    20521 ggccgaacgc cgtgtacttg ccgtcgagga acggagccgc gccgtccatg atgaagaact
    20581 gcgagcccgc cgagttcggc gtgccgctgt tcgccatcga gagaacgccg gcgacgtgct
    20641 tgtgcgagtt gaactcgtcc ttgatcttgt atccgggatc gccggttccg agatcgggag
    20701 ccttcgggtt cttcgacttg ggatcgccgc cctggatcat gaagcccttg atgatccgat
    20761 ggaacccggt cccgtcgaag tagccctggc gcgcgagctt cttgaagttg tccaccgtct
    20821 ggggcgcttc cttgtcccag aactcgatca ccatccgacc cttcgtggtc tccatgacgg
    20881 cgacctcgtc ggacgaggcc gcgctcttct tggccgcgcc cgccgcgaac gcgggctggg
    20941 cgccgaacgc cccgagcgcg agcatcacgc ccgtgtaccg aatgaatcct gacaccatgg
    21001 atcctccttg tcgtgcgtgg ggtgacccac gtcagtccgc acgatctttc gacttgagaa
    21061 gccgcacgat gcgccgctgg cgttccagct cgcgcttcgc cggctcagcg taagccggat
    21121 cgttgaggac cgcctcgaag accggaatgg cgtcctcgtg gcggcccagc ttgtccagcg
    21181 cctgggccca gcaaaaccgg agacacgcgc tggcggagtc accggccagg ccctcgcgcg
    21241 ccacttcgat cgcccgctgg tagcggctcg catcgtagta cgcgtaggcc atgttgcaat
    21301 acacaccctt gtagttctgc ggatccgcct tcgcgacctc gcggtacaga tccacggcgc
    21361 ggtccacctt gttcgacttc gcgtacgact gcgccaggta atagcggtcg acggtgccga
    21421 gcgaatcctg caccgattcg tagttcttga tggcgagcgg gtagcgctcg agcttgaagt
    21481 tgacgaacgc gagctgcttc ttcgcgtcca cgtcgagcgg cgccaaggtg aggacccgcg
    21541 tgtaggcggt cgcggcctcg gcaaagaggc ctccgcgcac ggcggtctcg gcgaggctcc
    21601 gcgcgatctc gggatcgccc ggaaggagct cgctggcccg gtagagcgcg gacatcgact
    21661 cggggatctt ggtctcggca gcgcacacct cgccgagctg ctggtacgcg cgggcctggt
    21721 ccgtgggact gcccttggtc atgcgcacgg tggcggcgca gatctcgcga gcctcgccgg
    21781 tctcgccgat ggcgacgagc gactgggcga gagcgcctcg cgtgcgcagg tcttgaggcg
    21841 cgagcgcgac agcctggcga tacgccgtcg aggcgcgctg ggcatcaccc atcgcggtgt
    21901 aggcatctcc gagcgagcgg aacgccccca ccatctgact cgagatctgc gtcgcgcgct
    21961 ggagcgatgg aagcgcttcc ttgtactggc cgcggagcac gttgacctgt ccgagccccg
    22021 cgagcgctcc ccgatcgttc gggtccgctt cgagcgcgag gcggtactcg tgctccgcat
    22081 cgtcgaggag attccggctc atgagatcgt ttccggccgc tacgtgatcg gcaggccgca
    22141 cgggggcagc ggcggtcgtt gcgggtgcca ccttgctcga gccgtgcgag gcgcagccgg
    22201 cggcgaggag gagcgcgatc aggacgtgcg atggatgatg cgaaggccgt cgtaccggcg
    22261 tcatttcgac ctccctggat cggaaccccg gcgccctgag ccggcgccgg cgggactcac
    22321 tggttcccgg tggtttgagc cgacgacttc ttctgctggt cgaggaagtc ctgaaggacc
    22381 tggatgctgc cgcgggcgct ctcggccacc gtggtcgtgg agtcgacggt cacgaccttc
    22441 ttccagtagt tgatggcctc gtgatagatc tgcgcgtccg cgaacgcgac ccccatgttg
    22501 tagagggcct cctggttgct ggcgtcgagc ttgagcgctt tcttgtacag ctcggtcgcc
    22561 tcggggatct ttcccattcc gtacttggcg tttccgagat tgcagatcgc gtggacgttc
    22621 gtggagtcga tcgagagcgc cttgttgtag gagaccacgg cgtcttcgat cttccccgac
    22681 tcggcctgga tcagacccaa attcacccag gcctcggcaa gcggcggctt gagctcggtc
    22741 gccttcttga acgacacgag cgcgtcgtca cgctttccct ggagcacgta gtagctgccc
    22801 atgttgaggt gcgccgaagg atcgttggga tcgagctgga tcgccttttt gaactcccgg
    22861 taggcgtcgt ccggacgctt caggtccatg tagaccgcgc cgaggctcac gtgcgctccc
    22921 ggctcgttgg gcacgagcgc ggacatcttc tggaaggccc cgagcgcttc ctggggcttc
    22981 cccgccttgc ggtacgcggt tcccagtgcg aaat
//
LOCUS       DASTKC010000186         7354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_387697, whole genome shotgun sequence.
ACCESSION   DASTKC010000186 DASTKC010000000
VERSION     DASTKC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387697"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..3112
                     /locus_tag="VFR25_07710"
     CDS             2..3112
                     /locus_tag="VFR25_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HET9940973.1"
                     /translation="MFIQRPVMTTLVMIGILIFGIIGYKQLPVSDLPKVDYPVISVSA
                     NLPGASPETMASAVATPLEKQFTTIAGIESMTSTSTQGATSITLQFALDRDIDAAAQD
                     VQAAIAKTLRSLPTGITPPSYQKVNPADSPILFLALTSEMLSLSQLDDYAENVIAQRL
                     STVDGVAQVSVFGAQKYAVRIQLDPRALASRGIPIGDVTDAIDAANVNLPTGILWGPS
                     RTATVMAGGQLQSAAEFGNIIVAVRDGAPVRLKDLGVVKDDVQNNRVASWYMGRRNIT
                     LAIQRQPGTNTVEVAAAVRRMLDTLRPQLPASVGVHDLFDRSEGIRHSVTDVQFTLLL
                     TLALVVLVIFLFLRNLPATVIPSLSLPLSLVGTFALMHLLGYSLDNLSLMALTLSVGF
                     VVDDAIVMLENIHRHIEMGKTPMQAAFEGSREIGFTILSMTLSLVAVFIPVLLLGGIL
                     GRLFHEFAVTISAAILVSGFVSLTLTPMLCSRFLKPEHATQHGRAYRTIEDLYDRMFR
                     AYDRSLGWVMDHRRATMAFSLAILVGTVVLFHIAPKGFIPSEDTGRIQASTESAQGTS
                     FTAMVEKQRQVAAIIGKDPNIAAYSSSVASGGGVSGTNQGRFIIRLKPRNERDKSADE
                     VIRGLQPKLSQVPGIRVYLQNPPVINVGGRFSKSQYQYTLQSGDLEDLYDASTALTTK
                     LQDMPGLQDVTSDLQISNPQVNVAIDRDRASQLGVSAQQIEEALYDAYGSRQVSTIFT
                     SSDQYWVILELLPEYQRDLTALQSLYVGSKNGTIVPLSSVARIQSGVGPLSVNHQGQI
                     PSVTISFNLREGVSLGTAVSDVDRIARETLPANVTGSFSGTAQVFQSSQQGLLVLLVL
                     AIFVIYLVLGILYESFIHPITILSGLPFAGFGALLTLVIFRTELSIYAFVGIILLIGL
                     VKKNAIMMIDFALDAERNEGKSPRDAIVEACKVRFRPIMMTTMAALMGTLPIAIGLGT
                     GSESRRPLGLAVVGGLAFSQLITLYVTPVIYTYLDAFTQRLRRRRGAEAPSPALDEGG
                     ALPVSS"
     gene            3238..3798
                     /locus_tag="VFR25_07715"
     CDS             3238..3798
                     /locus_tag="VFR25_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9940974.1"
                     /translation="MGEEVEAPEPALVARVRAGESSAYDELVRLHMGRVFAIILRVLG
                     QREDAEDVTQEVFLAVLEKLDTYDPRRPFAPWLYRIATNRALNARKARQLRRMDEIPE
                     SARAKGDEPDVATAKAEERERLARALQELPERQRLIVKLFDLEGFSSVEIGSMLGMAD
                     GTVRWHLLKARERLRRALAPMEEWHR"
     gene            3795..4193
                     /locus_tag="VFR25_07720"
     CDS             3795..4193
                     /locus_tag="VFR25_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940975.1"
                     /translation="MNMDPERDLELAAALRRLEGGSFLTEVEQTRIQNAIRIGAREAL
                     ARRRFAPRTPLEAIGGWWRAALPVAAAAALMLALVQPWRGRPAQEQASARSGLALVVA
                     GEAEEHDVARAIVGSPSEDLWVADTGGNGP"
     gene            4190..4618
                     /locus_tag="VFR25_07725"
     CDS             4190..4618
                     /locus_tag="VFR25_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9940976.1"
                     /translation="MNGTPGKPARVPPRVAAALLLIGMFAIGIVAGVALERVRDRRGR
                     DQHRVRDGVPVWALPERDQRRHWARVSDQLALTPEQRAQVDSILTRRAAQLEAARAAV
                     EPTMSAIMSSARSQIDSVLTKDQLARIQEMRRQRAKERGR"
     gene            4615..6003
                     /locus_tag="VFR25_07730"
     CDS             4615..6003
                     /locus_tag="VFR25_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9940977.1"
                     /translation="MRALPTAALAALLGLVLVTPVLAQMAAPDSVPAPDVVPRDQLLA
                     STQEPRPINLAEAVDLARRNSLLRVQARGRSRTAAAAVRSAYGAYLPTLSVTGGGTRQ
                     LPESGGRTRIENGQVIVLPATPWSYNTTISSSLQLFDGFRRLNELHQAHAEEDASETN
                     ENQQQFQAVLDAKQQYFNVLAWREAESAAIAQLEQAEQQLKAAVLRVRAGTATRSDSL
                     RSVIQVSDARLTLSEARNNVVVANASLTRAVGSDVPVTAASEDSTSLVGISVSEAELV
                     KLVDRSPSVQLAEAELAVASEGQKVARTGYIPTVNASYNYSGSGTERPFAFGTEDFDY
                     NGALRLSFNIPIFDQFVREESVVRANVAKDNAEASLKDAKLAAHQSLAQSLADYRNAE
                     ERVFTQTATLSTAEEDYRVQERRYQVGASTLLDLLTSQTTVNQARQTLIRARFDQRVA
                     KATLEALVGRSL"
     gene            6000..7250
                     /locus_tag="VFR25_07735"
     CDS             6000..7250
                     /locus_tag="VFR25_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9940978.1"
                     /translation="MSPENAGMVVERPGNGRKRRGLRRWIVIGLSVVVAAAAIYGVTH
                     RKKTTKTVIQTAAVERQDITVTVEANGSIEPINVVEVKSKASGQIMKMPVDVGSNVTP
                     NQLLVKIDERDVRNQFDQSQAALRAAQVSEQVALAQRKRSDQLAQDGVITATEHETAT
                     LAYESARSALVRARTDLDIARQKLEDATVRAPVAGTIIEKPVSVGTVISSATSSVSGG
                     TTLLKMADLKQIRLRSLVNETDIGNVKPGQPATVVVDAYPDRPFRGTVEKIEPVATVD
                     QSVTQFPVLVSISNEEGLLMPGMNGEVTILVEEQDDVLAVPIDAIRTTREAAEAGQAL
                     GLKPDDIRNELRNQAQTRMAAAQGGGAGAAADPAATGATAGRGTAMAQPGGAGAADTT
                     RGGWRRGRGDSTAAGGFGRGGGRR"
BASE COUNT         1220 a         2540 c         2477 g         1117 t
ORIGIN      
        1 catcttcatt caaagaccgg tcatgaccac gctcgtcatg atcggcatct tgatctttgg
       61 aatcatcggc tacaagcagc tgcctgtgag cgatctcccg aaagtcgatt acccggtgat
      121 ctcggtgagc gccaaccttc ccggcgcgag ccccgagacg atggcctcgg cggtcgcgac
      181 gccgctcgag aagcagttca ccaccatcgc cggcatcgag tcgatgacct cgacgagcac
      241 gcagggagcc acgtcgatca cgctccagtt cgcgctcgac cgggacatcg acgcggcggc
      301 gcaggacgtg caggcggcca tcgcgaagac gctccgatcg ctcccgaccg gaatcacgcc
      361 tccgtcctac cagaaggtga atcccgccga ttccccgatt ctcttcctcg cgctcacgtc
      421 ggagatgctc tccctctccc agctcgacga ctatgccgag aacgtgatcg cgcagcggct
      481 ctcgacggtg gacggcgtgg cgcaggtctc ggtcttcggc gcccagaagt acgcggtccg
      541 gatccagctc gatccgcggg cgctggcctc gcggggcatc ccgatcgggg acgtcaccga
      601 cgcgatcgac gccgcgaacg tgaaccttcc gacgggaatc ctctggggac cgagccgcac
      661 ggccaccgtg atggccggag gacagctcca gagcgccgcc gagttcggca acatcatcgt
      721 ggcggtgcgg gacggcgcgc cggtccggct caaggatctc ggcgtggtga aggacgacgt
      781 gcagaacaac cgcgtggcga gctggtacat ggggcggcgc aacatcacgc tcgccatcca
      841 gcgccagccg ggaaccaaca cggtggaggt ggccgcggcg gtccgccgga tgctcgacac
      901 cctccgtccc cagcttccgg cctcggtcgg cgtgcacgac ctgttcgacc ggtccgaggg
      961 gatccggcac tcggtcaccg acgtccagtt cacgctcctc ctcacgctcg cgctcgtcgt
     1021 gctcgtgatc ttcctcttcc tacggaatct tcccgcgacc gtcattccga gcctctccct
     1081 gccgctctcg ctcgtcggga cgttcgcgct catgcatctg ctcggctaca gcctcgacaa
     1141 cctctccttg atggcgctca cgctctcggt cgggttcgtc gtggacgacg ccatcgtgat
     1201 gctggagaac atccaccggc acatcgagat ggggaagacc ccgatgcagg cggccttcga
     1261 aggctcccgc gagatcggct tcaccattct ctcgatgacg ctctcgctcg tggccgtgtt
     1321 catcccggtg ctcctcctgg gcggaatcct cggacggctc ttccatgagt tcgcggtcac
     1381 gatcagcgcg gcgatcctgg tctccggctt cgtgtcgctc acgctcacgc cgatgctctg
     1441 tagccgcttc ctcaagcccg agcacgcgac gcagcatggc cgcgcctacc ggacgatcga
     1501 ggatctctac gaccggatgt tccgggccta cgaccgctcc ctcggctggg tcatggatca
     1561 caggcgcgcg acgatggcgt tctcgctcgc gatcctggtc ggcacggtcg tgctcttcca
     1621 catcgcgccg aagggcttca tcccgagcga ggacacgggg cggatccagg cgagcaccga
     1681 gtcggcgcag gggacgtcct tcaccgcgat ggtcgagaag cagcggcagg tggccgcgat
     1741 catcgggaag gatccgaaca tcgcggccta cagctcgtcc gtggcttcgg gcggaggcgt
     1801 ctcggggacg aaccaggggc gcttcatcat ccggctcaag ccgcggaacg agcgcgacaa
     1861 gtccgcggac gaagtcatcc gcgggctcca gcccaagctc tctcaggtgc cgggcattcg
     1921 cgtgtacctc cagaaccctc ccgtcatcaa cgtcggcgga cggttctcga agagccagta
     1981 ccagtacacc ctgcagagcg gcgacctgga ggacctctat gacgcctcga cggcgctcac
     2041 cacgaagctt caagatatgc ccggcctcca ggacgtgacg agcgacctcc agatctcgaa
     2101 cccgcaagtg aacgtggcga tcgaccggga ccgcgcgtcc cagctcggcg tctcggcgca
     2161 gcagatcgag gaggcgctct acgacgccta cggctcgcgc caagtctcca ccatcttcac
     2221 ctcgagcgac cagtactggg tcatcctcga gctcctcccc gagtatcaaa gagacttgac
     2281 cgcgctccaa agtctctatg tggggtcgaa gaacggcacg attgtcccgc tgagttcggt
     2341 ggcgcggatc cagtccgggg tcgggccgct gagtgtgaac caccaggggc agatcccctc
     2401 ggtcacgatc tcgttcaacc ttcgcgaggg cgtctcgctg ggaacggcgg tctcggacgt
     2461 ggaccgcatc gcgcgggaga cgctccccgc gaacgtcacc ggcagcttct ccggcacggc
     2521 ccaggtcttc cagtcgagcc agcaggggct cctcgtcctc ctcgtgctcg ccatcttcgt
     2581 gatctatctc gtgctcggaa tcctctacga gagcttcatc catccgatca cgatcctgtc
     2641 ggggcttccg ttcgcgggct tcggcgcgct cctcacgctc gtgatcttcc ggaccgagct
     2701 cagcatctac gcgttcgtgg ggatcatcct tctcattggg ctcgtcaaga aaaacgcgat
     2761 catgatgatc gacttcgcgc tcgacgcgga gcggaacgag gggaagtcgc cacgcgacgc
     2821 gatcgtcgaa gcctgcaagg tccgatttcg cccgatcatg atgaccacca tggccgccct
     2881 catgggcacg ctcccgatcg cgatcgggct cgggaccggg tccgaatccc ggcgcccgct
     2941 cggcctcgcc gtcgtggggg gactggcctt ctcccagctc atcaccctct acgtcacccc
     3001 cgtgatctac acctacctcg atgccttcac gcagcggctc cgccgccgcc ggggcgcgga
     3061 agcgccctcc ccggcgctcg atgaaggggg cgccctcccg gtatcctcgt aagcggggtc
     3121 ccgcgcgggt gcatgccgcg gatcgcgggg ccaacggccg acgggcgccg gatgtaatcc
     3181 cgtacgagcc ctgaccccgc ggccacgagg ctcgtgggct ttagcccgga ggaaggcgtg
     3241 ggggaggaag tcgaggctcc ggaaccggcg ctcgtcgcgc gcgtgcgcgc gggcgaatcg
     3301 tcggcgtacg acgagctggt ccggctccac atgggacgcg tcttcgcgat catcctccgg
     3361 gtgctcggcc agcgcgagga cgcggaggac gtgacgcagg aggtcttcct cgccgtcctc
     3421 gagaagctcg acacgtacga cccgaggcgt cccttcgcgc cgtggctcta ccggatcgcc
     3481 acgaatcgcg cgctcaacgc aaggaaggcg cgccagctca gacgcatgga cgagatcccc
     3541 gagtcggcgc gggccaaagg cgacgagccg gacgtcgcga cggcgaaggc cgaggagcgc
     3601 gagcggttgg cgcgagcatt gcaagagctg cccgagcgac agcggctcat cgtcaagctg
     3661 ttcgatctcg aaggattctc gagcgtggag atcggctcca tgctcggcat ggcggacgga
     3721 accgtccgct ggcatctgct gaaggcacgg gagcggctac gccgcgcgct cgcgccgatg
     3781 gaggagtggc accgatgaac atggaccccg agcgcgatct cgaactggcc gcggcgctgc
     3841 gccgcctcga agggggatca ttcctcaccg aggtggagca gacccgaatc cagaatgcga
     3901 tccggatcgg cgcgcgcgaa gcgctcgcac ggcgccgctt cgccccgcgc acgccgctcg
     3961 aagccatcgg aggatggtgg agggcggcgc tgcccgtcgc ggccgcggcc gcgctgatgc
     4021 tcgccctggt ccagccctgg cgcgggcgtc cggcgcaaga gcaggcgagc gcgcgctccg
     4081 gcctcgcgct cgtcgtggcc ggcgaggcgg aggagcatga cgtggctcgc gccatcgtgg
     4141 gcagcccgtc cgaggacctg tgggtcgccg acaccggagg aaatggaccg tgaacggaac
     4201 gccgggcaag ccggcgcgcg tcccgcctcg ggtcgccgcc gcccttcttc tcatcggaat
     4261 gttcgcgatc ggcatcgtgg ccggcgtcgc gctcgagcgc gtgcgcgacc gccgagggcg
     4321 ggatcagcat cgcgttcgcg acggcgtgcc tgtctgggcg ctccccgagc gggaccagag
     4381 gcggcactgg gctcgcgtct ccgaccagct cgctctcacg ccggagcagc gcgcgcaggt
     4441 cgattcgatc ctgacgcgcc gcgcggcgca gctcgaggcg gcgcgcgcgg cggtggagcc
     4501 gaccatgagc gcgatcatgt cgtccgcgcg cagccagatc gacagcgtcc tgacgaagga
     4561 tcagctcgca cggatccagg aaatgcggcg gcagcgcgcg aaggagcgtg gccgatgagg
     4621 gctctcccca cggcggcact ggccgcgctt ctgggtctcg tccttgtcac gcctgtgctc
     4681 gcgcagatgg ccgcgcccga ttccgtcccg gcgccggacg tcgttccgcg cgatcagctc
     4741 ctcgcgtcga cgcaggaacc caggccgatc aatctcgccg aagccgtcga tctcgcgcgg
     4801 cggaactcgc tcctccgggt gcaggcccgg ggacgttcgc gcacggcggc ggcggcggtc
     4861 cgctccgcgt acggtgcgta cctacccacg ctcagcgtca ccgggggcgg cacgcggcag
     4921 cttcccgagt cgggcggtcg cacccggatc gagaacgggc aggtcatcgt ccttccggcg
     4981 accccctggt cctacaacac gacgatctcc tccagtctgc agctcttcga cggattccgg
     5041 cgtctcaacg agctccatca ggcgcatgcg gaagaggacg cgtccgagac gaacgagaac
     5101 cagcagcagt tccaggcggt cctcgacgcg aagcagcagt atttcaacgt gctcgcgtgg
     5161 cgcgaggcgg aatcggcggc catcgcccag ctcgagcagg cggagcagca gctcaaggcc
     5221 gcggtcctcc gcgtgcgcgc gggcacagcg acccgctcgg actcgctacg ctcggtcatc
     5281 caggtctccg atgcacggct gacgctgagt gaggcccgga acaacgtcgt cgtcgcgaac
     5341 gcgtcgctca cgcgcgcggt cggctctgac gtgccggtga cggccgcgag cgaggactcg
     5401 acgagcctgg tggggatttc cgtcagcgag gcggagctgg tcaagctcgt cgaccggagt
     5461 ccgtccgtgc agctggcgga ggcagaactg gccgtcgcgt cggagggcca gaaggtggcg
     5521 cgcacgggct acatccccac ggtgaacgcc agctacaact actcgggaag cggcaccgag
     5581 cggccattcg ccttcggcac cgaggacttc gactacaacg gcgcgcttcg cctctcgttc
     5641 aacattccca tcttcgatca gttcgtccgc gaggagagcg tggtccgggc caatgtggcg
     5701 aaggacaacg ccgaggcctc gctcaaggac gccaagctcg ccgcgcatca atccctcgcg
     5761 cagagcctgg ccgactaccg gaatgccgag gagcgtgtgt tcacgcaaac cgccacgctc
     5821 agcaccgccg aggaggacta tcgcgtgcag gagcggcgct accaggtcgg agcgtcgacg
     5881 cttctcgatc tcttgacctc gcagacaacg gtcaatcaag cacgccagac cttgatccgc
     5941 gcgcggttcg accagcgcgt cgccaaggcg accctcgagg cactcgttgg gagatccctg
     6001 tgagcccgga gaatgccggc atggtggtgg agcggcccgg aaacggccgg aagcgccgcg
     6061 gcctccggcg gtggatcgtc atcgggcttt ccgtcgtcgt ggccgctgcc gccatctacg
     6121 gcgtcacgca tcggaagaag accaccaaga cggtcatcca gaccgcggcc gtcgagcgcc
     6181 aggacatcac ggtcaccgtc gaggcgaacg ggtccatcga gccgatcaac gtcgtcgagg
     6241 tgaagtcgaa ggcgtcgggg cagatcatga agatgccggt cgacgtcggc tcgaacgtga
     6301 cgccgaatca gctcctcgtg aagatcgacg agcgcgacgt gaggaaccag ttcgaccaga
     6361 gccaggccgc gctccgcgcc gcgcaggtct cggagcaggt cgccctcgcc cagcggaagc
     6421 gctccgacca gctcgcccag gacggtgtga tcacggcgac ggagcacgag accgcgacgc
     6481 tcgcctacga gagcgcgcgc tccgccctcg tgcgcgcgcg aaccgatctg gacatcgcgc
     6541 gccagaagct cgaggacgcg accgtccgcg cgccagtggc cgggacgatc atcgagaagc
     6601 cggtctcggt gggaacggtc atctcgtcgg cgacctcgtc ggtgagcggc gggacgacgc
     6661 ttctcaagat ggcggacctg aagcagatcc gcctccgctc gctcgtgaac gagaccgaca
     6721 tcggcaacgt gaagccggga cagccggcga cggtcgtggt ggacgcgtat cccgatcgtc
     6781 ccttccgcgg aacggtcgag aagatcgagc cggtcgccac cgtggatcag tcggtgacgc
     6841 agtttcccgt cctcgtctcg atctcgaacg aggaggggct cctcatgccg gggatgaacg
     6901 gggaagtcac gatcctggtc gaggagcagg acgacgtgct cgcggtgccc atcgacgcga
     6961 tccgtacgac gcgggaagcg gccgaagcgg gacaggcgct cggactgaag ccggacgaca
     7021 tccggaacga gctccgcaat caggcgcaga cgcgcatggc cgcggctcaa ggcggcggag
     7081 ctggtgcggc tgccgatccg gccgcgaccg gcgcgaccgc tggacgcggt acggcgatgg
     7141 cgcagccggg cggcgcgggc gcggcggaca ccacccgcgg cggatggcgt cgcgggcgcg
     7201 gggacagcac cgccgcgggc ggctttgggc gcggcggcgg acggcgttga ggcggcggag
     7261 gacgcagtcg ccagatgcgc gtcgcgttcg tgaagcaggg ggacaagtac gtgccgcgca
     7321 tggtccgcat cggggtctcg aactacgact acgc
//
LOCUS       DASTKC010000187         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_391596, whole genome shotgun sequence.
ACCESSION   DASTKC010000187 DASTKC010000000
VERSION     DASTKC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391596"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFR25_07740"
     CDS             <1..1342
                     /locus_tag="VFR25_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HET9940979.1"
                     /translation="GGGLSGAAVAHALLARARGPLLVTVIGRAPLGEGTAYSSSHPLH
                     LLNVRSRGMSLVDGDDAHFVRWLAGRDGADADPDGFVPRRVYADYVRDTLAAAEGSSK
                     ASLERILGNVVGFDLSAEGVRVTLENGDTQSGDRAVLALGHPPPNRPIAGDLPLYDSP
                     RYVSDPWSAEAIAGLDPDAALLVIGTNLTMFDLAMTLEARGHRGVIHAVSRRGLTSLA
                     HGPAPSMGAPWNGTAPPRSARAALRALRGEARVEPAAWRGIVDSIRAQTPALWSSWPD
                     DERRRFLRHARPYWDIHRHRAAPEIHERIDHMRAEGGLRVSAARLTSLELRGNGVVAT
                     IAPRGGSAPVKLAVERVINATGPSVDYRTWDDPLVRALFHRGWIRPGPLGFGLDATAE
                     GAILDRDGRASTNLTTLGPTLRGSLWETTAVPEIRAQAARLAMRILTDLESRPA"
     gene            1339..2607
                     /locus_tag="VFR25_07745"
     CDS             1339..2607
                     /locus_tag="VFR25_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET9940980.1"
                     /translation="MTWPLPPFLWGVATSAYQIEGGIIGNDWTDWEREPGKVRGGVCS
                     GEACGSWEHWQRDLDRIQALGLNAYRFSIEWSRVEPEEGRFDEAALAHYRTILSGCRE
                     RGITPLLTLHHFTNPRWFLARGGWEERSNLAAFERYARLAGERFGDLVDLWITINEPE
                     VYGFYAYDAGVFPPGIRDRVRALRVIANLLEAHGLASQALREADRVDADGDGRAALIG
                     AAKHWVLLEARNPWSPVDQIAAVVQHGVFNVAVTRALTGGLIDLWIPGVPAIKRHVDA
                     LQGASDFLGVNYYTRWQVGMGGDGPMSARPGVPKSDLGWEILPDGLETALRSCAGFRV
                     PLMVTENGIADAADRWRPDFIRASLAALDRARAAGVDVRGYFHWSLMDNYEWHEAYEG
                     KFGLYARDAAGATGERPSAKVYTEEVARRR"
     gene            2681..3085
                     /gene="mscL"
                     /locus_tag="VFR25_07750"
     CDS             2681..3085
                     /gene="mscL"
                     /locus_tag="VFR25_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET9940981.1"
                     /translation="MLAEFKKFLLQTNALALAIGVIIGGAVGKVVSSLVSDILMPVIG
                     LLVPAGGAWRELSIVLSTKPDGTPANAITYGAFLGNIVDFVIIALCVFMITKALLKPA
                     PAEPGPPTKECPECKEIIPAAARKCRACTSAV"
BASE COUNT          467 a         1083 c         1079 g          489 t
ORIGIN      
        1 cggcggaggg ttgagcggcg cggccgtggc gcacgcgctc ctcgcgcgcg cgcgggggcc
       61 cctactggtc accgttatcg ggagggcgcc tctgggcgag gggaccgcct acagctcgtc
      121 ccatcccctc catctcctga atgtccgctc gcgcgggatg agcctggtcg acggcgacga
      181 cgcccacttc gtccgatggc tcgcggggcg ggacggagcg gatgcggatc ccgacggctt
      241 cgttccccgc cgcgtctacg ccgactacgt gcgcgacacg ctggccgcgg ccgagggctc
      301 ctccaaggcc agcctcgagc gcattctcgg aaacgtcgta ggcttcgacc tctcggccga
      361 gggagtccga gtcacgctcg agaacggtga cacgcagagc ggagaccgcg cggtgctcgc
      421 actcggccat ccaccgccga atcgtccgat cgccggcgat ctcccgctct acgacagccc
      481 gcgttacgtg agcgacccgt ggtcggcgga agcgatcgcg ggactcgatc cggatgcggc
      541 gctcctcgtg atcggcacca acctcacgat gttcgacctt gcgatgacgc tggaggctcg
      601 cggacatcga ggcgtgatcc acgcggtctc gcgacgcgga cttacctccc tcgcgcacgg
      661 tcccgcgccg tccatgggcg ctccgtggaa cgggacggct ccgccgcgga gcgcccgcgc
      721 agcgctccgg gccctgcgcg gcgaagcccg cgtcgagccc gcggcgtggc ggggcatcgt
      781 ggattcgatc cgtgcgcaga cgccggccct ctggtcctcg tggccggacg acgagcgccg
      841 caggttcctc cggcacgcgc gcccctactg ggacatccat cgccatcgcg ccgcgcccga
      901 gatccacgag cgcatcgacc acatgcgcgc ggaggggggg ctccgtgtga gcgcagcgcg
      961 tttgacctcc ctcgagttgc gcgggaacgg cgtcgtggct acgatcgctc cgcgcggcgg
     1021 cagcgccccc gtgaagctcg cggtggagcg cgtgatcaac gcgaccggcc ccagtgtcga
     1081 ttaccggacg tgggacgatc cgctcgtgcg cgcgctcttc catcgaggat ggatccgtcc
     1141 cgggccgctc ggcttcgggc tcgacgcaac cgcggaggga gcgatcctgg accgggacgg
     1201 ccgcgcttcg accaacctca ccacgctcgg gcccaccttg cgcgggtcgc tctgggagac
     1261 gaccgcggtt ccggagattc gcgcccaggc cgcgcggctc gcgatgcgaa tcctcacgga
     1321 tctggagtca cgtcccgcgt gacctggccc ctccctccct tcctctgggg cgtcgccaca
     1381 tcggcgtatc aaatcgaagg aggcatcatc ggaaacgact ggaccgactg ggagcgtgag
     1441 cccggaaagg tccgcggcgg cgtctgctcc ggcgaggctt gcggaagctg ggagcactgg
     1501 cagcgcgacc tcgaccggat ccaagcgctc ggcctgaacg cctaccgctt ctcgatcgaa
     1561 tggagtcgcg tcgagccgga ggaagggcgc ttcgacgagg cagcgctcgc ccactatcgg
     1621 accatcctct ctggatgccg tgagcggggc atcacgccgt tgctgacgct ccatcacttc
     1681 acgaaccctc gatggttcct cgcgcgcgga ggatgggagg agcgttccaa cctggcggca
     1741 ttcgagcgct acgcccgtct ggcgggcgag cgcttcggcg atctcgtcga cctctggatc
     1801 acgatcaacg agcccgaggt gtacggcttc tacgcgtacg acgcgggcgt cttccccccc
     1861 ggcattcgcg accgcgtgcg ggcgctccgc gtgatcgcga acctcctcga ggcgcacggc
     1921 ctggcgtcgc aggctctgcg cgaggcggat cgcgtcgacg ccgacggcga tggccgcgcg
     1981 gccctcattg gcgccgcgaa gcattgggtg cttctcgagg cacggaatcc ctggtcgccg
     2041 gtggaccaga tcgccgccgt cgtgcagcac ggcgtcttca acgtcgccgt cacgcgcgcg
     2101 ctcacaggcg gactcatcga tctgtggatc ccgggcgtgc cggcgatcaa gcgacacgtc
     2161 gacgcgctcc agggcgcatc cgatttcctc ggggtcaact actacacccg atggcaggtc
     2221 ggtatgggcg gagacgggcc catgagcgcc cggccgggcg ttccaaagag cgatctcggg
     2281 tgggagatcc tgcccgatgg cctcgagacg gcgcttcgct cctgcgcggg cttccgcgtt
     2341 ccgctcatgg tgaccgagaa cggcatcgcc gacgccgcgg atcggtggcg gccggacttc
     2401 atccgggcat cgctggccgc actggaccgc gcgcgcgccg cgggagtcga cgttcgcggc
     2461 tacttccact ggtcgctcat ggacaactac gagtggcacg aagcgtatga agggaagttc
     2521 ggactctacg cgcgcgacgc tgcgggagcg acaggggagc ggccgagcgc gaaagtctac
     2581 acggaggaag tggcgcgaag gcgttgacgc gtccgctccc gcgggcgcat ggttggtgat
     2641 cccgttcccg gcttccaggc ccacgtcagg gaggcgatcc atgctcgcgg aattcaagaa
     2701 attcctgctc cagacgaatg cgctcgcgct cgcgatcggc gtcatcatcg gcggcgcggt
     2761 cggcaaggtc gtgagctcgc tggtgagcga catcctcatg ccagtgatcg ggcttctggt
     2821 tcccgcgggc ggcgcgtggc gcgagctcag catcgttctc tcgacgaaac cggatggaac
     2881 gccggccaac gcgatcacct atggcgcgtt cctggggaac atcgttgatt tcgtgatcat
     2941 cgcgctctgc gtcttcatga tcacgaaggc gctcctgaag ccggcacccg ccgagcccgg
     3001 tccgccgacg aaggaatgcc ccgagtgcaa ggagatcatc cccgccgcgg cgcgcaagtg
     3061 ccgcgcgtgc acgagcgcgg tataggtcaa cgcgattgga acgtagtgac ggtcctgg
//
LOCUS       DASTKC010000188        13831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_392946, whole genome shotgun sequence.
ACCESSION   DASTKC010000188 DASTKC010000000
VERSION     DASTKC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392946"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFR25_07755"
     CDS             <1..673
                     /locus_tag="VFR25_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9940982.1"
                     /translation="QDPHRSAIDYHLSALRTIGVTPVQTRMPRLYLSDVERAEGAARL
                     DQALPPGEPRVGLHVGNRWPAKRWPEERFAALLRALSKVGARGVVLAGPGEESLARRV
                     ADGARTGGRAPAPVISDLPLRSYFAVVASLDALVTNDGSPLHVGPAVQVPTVGILGPT
                     VPEIWFPYAREDGHQLLCKPIWCRPCHRHECARLDCLEWVEVGEALRATESALAHGGR
                     VRASA"
     gene            660..1748
                     /locus_tag="VFR25_07760"
     CDS             660..1748
                     /locus_tag="VFR25_07760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9940983.1"
                     /translation="MQAPEVDAATASSPLAGARRLLVIRHRAAGDLLLTTPAFRALRA
                     GLPACRIEVLVGRGQGELLEGNVDVDAAIELDRRSIGSQLKLYARLARGGWDAVIDLV
                     SNPRSAWMTALTRARVRAGYAIPGRDWAYTHSVPREPMGPSGPRLRYAPEAALDVVRA
                     LGIPDRGLDLTFHVSQRVRAEALDAMMKLGLRQVVLCHPGGSWPSKTWIAGRFTSAMD
                     VLAKDSNLRVVWMWGPGERDLAEQCQRGMNAESTLAPAMGWQEMGAWMRFASVWVGND
                     SGPKHLAVALGVPTVTVFGPTHPTTWHPPTGPHRAVMARGLACLHCNANVCPLPGERF
                     HRCMRDVTVDDVVRAVREVRNPPRREAW"
     gene            1745..2521
                     /locus_tag="VFR25_07765"
     CDS             1745..2521
                     /locus_tag="VFR25_07765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9940984.1"
                     /translation="MKEPLSVLVPTKNEEPNIRAVLESVRFAEEVVVVDSGSTDGTLP
                     IARSIADRVLEHEYVNSAAQKNWAIPQLTHRWILIVDADERVTPELRAEIERVLADPA
                     RLDGYWIHRDNHFLGKPIRTAGWQRDKVLRLFDRTKGAYEPLHVHAEVVIRGRVGTLQ
                     HKLVHHTYRSLDQYFEKFGRYTRWSAADLKARGVRASGMRLLVRPWLRFLRMYVLEGG
                     FREGRAGIVLCMLAAFSVFTKYARRWEDEIRGGPPTPPAP"
     gene            2569..3645
                     /locus_tag="VFR25_07770"
     CDS             2569..3645
                     /locus_tag="VFR25_07770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940985.1"
                     /translation="MLREALAAVGAQSAIVAVSPQAGGLVRTAGLLARWVARGGGAGA
                     DAILVPSFAHRDVPLARPLARLAGLPVVFDPLVSRWDTQVGDLGRVRAGSLTAARLRA
                     SDKAALHGADLVLCDTWEHGDFLSAEYGVARRKLARIPVGADHLAFGRGSRRAQSGHS
                     KAGDALDVVYIGGFLPLHGVDVVIDAATLLERRHGPGIARFTLIGSGMTAHAADREIA
                     ARGLRSVRHVPRMAYPDAIDALARADVALGIFGTTAKAARVVPHKAYQALAVGVPLVS
                     RRSRAMAEFVREEEEFLAVPAGDAAAVAAGIERLASDPALRARVGAAGRQAAVDRVSP
                     ERIGPLLLEAVATARAATSPRRRR"
     gene            3642..4760
                     /locus_tag="VFR25_07775"
     CDS             3642..4760
                     /locus_tag="VFR25_07775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9940986.1"
                     /translation="MTGTAPRALRILHVDSERTWRGGERQVLELMRRQRARGDEPHLA
                     APRGSALSSRASSEGFPGHAVPMRGAWDIGSALALARLHRQLRPDVVHWHAARAHALG
                     AIASLFAPGPARVLSRRVDFPVRGSAGSRLLYGLPRDATIAISEGVRDALVRSGLDPA
                     SIDVVQSGIDLTPFEAPFDRSAIRSRLGTQEGDFLVLQVAALAPHKSQKDLLDAARIV
                     LDERPDARIWVAGEGILRDELERQHGALDLGDRVRLLGFRDDVPDLLRAADLFCVSSY
                     LEGMGTATLDAMAVGLPVVATRVGGIPEIVEDGVTGLLVAPRDPQALAGAILALARDP
                     ARRRAMGDGARRRVRAFSADRTEEETRRVYLKAIGAVD"
     gene            4822..6207
                     /gene="rseP"
                     /locus_tag="VFR25_07780"
     CDS             4822..6207
                     /gene="rseP"
                     /locus_tag="VFR25_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET9940987.1"
                     /translation="MTEALPVLFYGALLLGVLVLVHELGHFLVAKWLGVRVISFSIGM
                     GPRLFGFTKDGTDYRISALPLGGFVRMAGDSPEADERTGAPDEFLSKPWWARALITAA
                     GPGANLVFALLVNMAIFLIGVEGRDYSTRVSRVKAGSVAESIGLQARDHIVAYGGHPA
                     RTVSELSDAIETAGVDKSKSSQALPLTIDRNGETKTLALRPKDIDAFADGLDWDMGTQ
                     VGQVLIGYPAYESGLREGDEILKIDGQRVLLWTDLSTILRSKPSAARTLLVKRGDQTF
                     EVHLKTSPEGTIGISPPETMTYKQTFPPMKAITLGVQQTFWVTGQIYSGLWGFVTDPV
                     RLHTTVAGPIAIAQVARQQAKGGLQQLVTFASFISLALMAMNLLPIPILDGGHILFAF
                     IEAARRRPLSLKTQLFFQRIGLFVLMGLVVFAFYNDLSRVGQRRRAEADIDRRLQTTS
                     PSDTAAATHEP"
     gene            6234..8237
                     /locus_tag="VFR25_07785"
     CDS             6234..8237
                     /locus_tag="VFR25_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9940988.1"
                     /translation="MRGRSAPLASIRLFLFLASVVLAVLCAAPRSARAAADDEAELSF
                     ERETGKVEIVGNESVEKGRLKGLLRTRSKSFWKPWTKHPLRQDYLRADAATIESFYRR
                     TGYLYARVDSVVARPTKDPKKSDVVFYVHEGPRAMIKEILMTGLGPMSDREVREALLY
                     QVGSPLELGTLAASRDSITYQYGDRGYALVSVSDSLRVDSTQVSVLYAIRPGPKVRMG
                     SIEVEGTNKTKPSYVTRELLFKPGDVLLRSKLIQSQQRIYDSGLYSDVQMELGKVDSV
                     TSASDIAVTVRERKMGWVDAGIGYGTIDQLRLTGQWGQRNIFRSSMRLVATGRLGVQI
                     DTNPLHTHAGDRRIDLALTHPWPLGVRVQTTVGVYAEDEPIIQLTDQYPVQAIGASLV
                     LSSPMFKYGRNAASYELRHVTQDSLLTQSTTNDSSYTTHRIALTAERDTRLNIFDPMN
                     GSDVYGRVEFVAGTIPGTAQFTTSTLQATKYLRRGRVVLATRIRGGLIEPWRPSGPDT
                     SKTEVPLELAQIPNEDRFRTGGASTVRGYYENELGTREVHTTTASGDSVYSVIRGGQA
                     QLLISAELRFPLLWIFSGAAFFDGGNVWERPEDIKPENIFSFGNGAGYNDMRYSVGLG
                     LRVGTPIGPIRLDYGWKLRTPRPDAPDVNPNPGLLHFSLGHAY"
     gene            8239..12243
                     /locus_tag="VFR25_07790"
     CDS             8239..12243
                     /locus_tag="VFR25_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9940989.1"
                     /translation="MSRSTLEPPVREARERRPFHASTVFRAVLAVVLGVLALAAFAIA
                     SLLFLFRTGNPELTSRIVAEVNRIATSDSTRFTSSQVRGTLFRGAVIENPKLLVQTPD
                     GEVAWAQARSLRVDFDLIAFLFHQRQDLRIELDGPRIDLVHDRRGELVMPRFARKKKG
                     QGKGLGGTRVVIAVKNGAFSMDRMKTRYGKIGGVATLISGPEHSTIILDRLSGVSVTP
                     GRPGRLELSGELDMEKATLKARPLEIGLGSTHITAEVDWDLEKARVREGTLDLHPLEL
                     REFFKSLEIATEDGTARGEVTFSGTPTDGKARVCLAGTFAGEPVDTLVLEARSRPGAV
                     DFSGLNLRVRSTNVTGGGTFDTRGVLNTDLAFRNVNPKVLPWWKSPENTPDGSLAGRG
                     RFTVRRAAPNIDVLASVDLMPSRLGKLQISRGHVRVATHPNGSAQLDSSWIEIPGSRI
                     VAAGTLGKDGALAATAHAAVHNLEGMNVLLAAPQIEAGHGRVDAAFSGTIEAPQYQAQ
                     MDLFDTRLKNGIGGDSLTVEVQGKLKPELDLIADAGIRGLKAGTRPLGNVVATVSGGK
                     TLRIERYRQALGDTLITLGGKVIFAEQGAEALVDSVALTAGEYHVRSRGPVKVGFFGS
                     RVRVSDLALDLDPGTMHLDLDYNPSGDAIDARGSIADVDLGRIPGLAHGAIRYAGVVH
                     GDFLATGPLKDPDVSLRLAVVEPTVGDIVGDSVRVDLDYAPGVLTVEQAAWTSQASRL
                     AASGTIRPHLALQEWLKAIAKGDRSWSKRADLALTASLDSFDLKLVAPADTSLRSLQG
                     IATGKARISGTTSDPVMGVEGRVSSMSYRGVQGEIAGVDLAYENRRLKINRFDVKQNE
                     SISQVRGELPLDLGIYAEDRVMDKEPMTLTVDIPNGDFSIARILLPDLIGYSTGHYTA
                     SAVVSGTPSDARVTGSFKLTGARIRPAGRDEVLTNVESEATFDQKQLTVTKLTAKQGE
                     KGKLEANGLWRWPTGAKAEKPAFGPPGTYEFHVIGTDFTATDRSTYAVRFTGKIDIVN
                     GKDPLGGQRPLITGHGTVTKGELTLDLSKPPSTPSEPLPFLYSVNVDLPGNFFYRNLD
                     AEVELETVDGPLSFKNEGDGDLALGTLEVRGGKYYVMTREFRNLQGTISFNNPDRIDP
                     DVNVVGETQIPDPSSTEGSRTVYLALTDRVSRLKVRVYDDAGTPPNDLWKALAFGQFV
                     PMTGVSTEAAATGGVDPSVAVPISNYLFQNVERWIGSSGFIDTIDLRGSGGAADGSKS
                     TSALSMVGVGKYVTPQFYFKYSKDFSAKAEQEISADYRVTRHLLLKGQQIRSTDPARP
                     PQEYNLDLKIRLEY"
     gene            12251..>13831
                     /locus_tag="VFR25_07795"
     CDS             12251..>13831
                     /locus_tag="VFR25_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9940990.1"
                     /translation="MELNPVQRQAVEHPGGPLLILAGAGSGKTRVLTGRVAHLVRERG
                     VHEGSILAFTFTNKAAREMRERVESLLETDELRTWVGTFHATCVRILRRHAELLGFPK
                     SFVIYDTDDQRTLLRELLRDEGGDDRTLTPAGASSRISRLKNEGVTPEAFESQIRSPI
                     DRKLAPVYAKYTKALRDRAAMDFDDLLLFTVRLFDADEDVHRSYAGRFQHVLVDEYQD
                     TNSIQFELIERLSRVHRNLTVVGDDDQSIYRWRGADVGNILSFEKQFPDATVLRLTQN
                     YRSTRTILRAANGVVKHNAGRKEKELWTENEAGNPLTLHVLPDEEAEGEKVVSLLLEA
                     RRRDDRSNGDFVVLYRTNAQSRAVENALRRSAIPYQITGGISFYERREVKDVLAYLRA
                     TVQPRDTVAWLRVINVPGRGIGKTTVDRLREFMDARGLTVPEALAHPELVQAVGPTSS
                     RKLLEVAHLFESLKPLLTLSPPACVTELVARVRYREYLQEEEPAEAAERIENVEELIA
                     GAEAYARRVEEPSVEGFLA"
BASE COUNT         2186 a         4751 c         4717 g         2177 t
ORIGIN      
        1 ccaggacccc caccgttccg cgatcgacta ccacctgtcg gccctgcgca cgatcggcgt
       61 caccccggtc cagacgagga tgccgcgcct ctacttgagc gacgtggagc gagcggaagg
      121 tgccgcgcgt ctcgaccagg cgttgcctcc cggggagccc cgtgtcggcc ttcacgtcgg
      181 gaatcgatgg ccggccaagc gctggccgga ggagcggttc gcggcgctcc ttcgtgcgct
      241 ttcgaaggtc ggggcgcgag gggtcgtgct cgcagggccg ggtgaggagt cgctcgcgcg
      301 acgggtggcc gatggcgccc gcacgggagg ccgggctccg gccccggtga tttccgacct
      361 tccacttcgg agttacttcg ccgtcgtcgc gtcgctcgac gctctcgtga cgaacgacgg
      421 cagcccgctc cacgtgggac ccgctgtcca ggtgcccacg gtcggtatcc tcggcccgac
      481 cgtgcccgag atctggttcc cgtacgcgcg agaggacgga catcagctcc tctgcaagcc
      541 gatctggtgc cgtccctgcc accgccacga gtgcgcgcgg ctcgactgcc tcgagtgggt
      601 cgaggtcggc gaggcgctcc gggcgaccga gagcgcgctc gcgcatggag ggcgagttcg
      661 tgcaagcgcc tgaagtggac gcggccaccg cttcaagccc ccttgcgggt gcgcgacggc
      721 tcctcgtcat ccgtcaccgt gctgcgggag atctcctgct cacgactccc gcctttcgcg
      781 ctctgcgcgc cgggctgccc gcgtgccgga tcgaagtcct ggtcgggcgc ggccagggtg
      841 agcttctcga agggaacgtg gacgtggatg ccgcgatcga gctcgaccgg cgctccatcg
      901 gctctcagct caagctctac gcgcgactgg ctcgtggcgg atgggatgcg gtcatcgatc
      961 tcgtgtcgaa tccgcggagc gcttggatga ccgcgctgac gcgtgctcgg gtgagggcag
     1021 ggtacgcgat cccgggacgc gattgggcgt atacgcattc cgttcctcgc gaacccatgg
     1081 gccccagcgg accaagactc cgatatgcgc cggaggccgc actcgacgtc gttcgcgcac
     1141 tcggaatacc ggatcgggga ctcgacctca cgtttcacgt ctcccagcgt gtccgggctg
     1201 aggcactgga cgcgatgatg aagctcgggc ttcgccaggt cgttttatgc cacccggggg
     1261 gatcgtggcc ttccaagaca tggattgccg ggcgcttcac gagcgccatg gacgttctgg
     1321 cgaaggactc gaacctcagg gtcgtctgga tgtgggggcc cggcgagcgg gacctggccg
     1381 agcaatgcca gcgcggcatg aacgcagagt ccactctcgc gccggccatg ggatggcagg
     1441 agatgggcgc atggatgcgg tttgcgtccg tctgggtcgg gaacgactcg gggccgaaac
     1501 acctcgctgt ggcgctcggc gtgccgaccg tgaccgtgtt cggacccacg caccccacga
     1561 cgtggcatcc tccgaccggc ccgcaccgcg ccgtcatggc ccgcggcctc gcctgcctcc
     1621 attgcaacgc caacgtctgc ccgcttcctg gcgagcgctt ccatcgctgc atgcgtgacg
     1681 tcaccgtgga cgacgtcgtg cgcgccgtcc gggaagtacg gaatccccca aggcgggagg
     1741 cctggtgaag gaaccgctca gcgttctcgt tcccacaaag aacgaggagc cgaacatccg
     1801 cgcggtcctc gaatcggtgc gcttcgcgga agaggtcgtc gtcgtggact cggggagcac
     1861 ggacgggacg ctcccgatcg cgcgctccat cgccgaccgc gtgctcgagc acgagtacgt
     1921 gaactcggcg gcgcagaaga actgggcgat cccgcagctc acgcaccgct ggatcctgat
     1981 cgtggacgcg gacgagcgcg tgacgcctga gctgcgcgcc gagatcgagc gcgtcctggc
     2041 cgacccggcc cgcctcgacg gatactggat ccaccgcgac aatcactttc tcggcaagcc
     2101 catccgcacc gcgggctggc agcgggacaa ggtgctccgt ctcttcgatc gcacgaaggg
     2161 ggcctacgag ccgctccatg tgcatgccga ggtcgtgatc cgggggcgcg tcggcacgct
     2221 gcagcacaag ctcgtccacc acacgtaccg gagcctggac cagtacttcg agaagttcgg
     2281 ccgctacacg cgatggtccg ccgccgatct caaggcgcgc ggcgtgcgcg cgtcggggat
     2341 gcgactcctg gtgcggccct ggcttcgctt tctccgcatg tacgtcctcg aggggggatt
     2401 ccgcgaaggg cgcgccggca tcgtcctctg catgctcgcg gcgttctcgg tcttcacgaa
     2461 gtacgcgcgg cgatgggagg acgagatccg aggcggtccg cccacgcccc ccgcgccgtg
     2521 acggtcgtct tcttcggagc gtcggacccc gcctatccga ggaatgtcgt gctccgcgag
     2581 gccctggcag cggtcggcgc tcagtcggcg atcgtcgcgg tttcgcccca agctggcggt
     2641 ctggtccgca cggccgggct cctcgcgcgc tgggtcgcgc gcggcggagg agccggggcg
     2701 gacgcgatcc tcgtgccctc gtttgctcat cgcgacgttc ccctcgcgcg accgctcgcg
     2761 cgcctcgccg gcctgccggt cgtcttcgat ccgctcgtgt cgcggtggga cacgcaggtc
     2821 ggagatctcg ggcgcgtccg cgccgggagc ttgacggcgg cgcgactccg cgcgagcgac
     2881 aaggcggcgc tccacggagc cgacctcgtt ctctgcgaca cgtgggagca cggcgatttc
     2941 ctgagcgcgg aatacggcgt ggcgcgccgc aagctcgcgc gcattcccgt gggtgcggat
     3001 cacctcgcgt tcggccgtgg ctcgaggcgc gcacagagcg ggcactcgaa ggcgggcgac
     3061 gcgctcgatg tcgtctacat cggcggcttc ctgccgctcc acggcgtgga tgtggtgatc
     3121 gacgcggcga cgctactcga acggcggcac ggtccgggca ttgcgcgctt cacgctgatc
     3181 ggcagcggga tgaccgccca tgcggccgat cgcgagatcg ctgcgcgagg gcttcgctcc
     3241 gtgcgacacg ttccgcgcat ggcttacccc gatgccatcg acgcgctcgc acgcgcggac
     3301 gtggcgctcg ggatcttcgg gacgacggcc aaggccgcgc gggtcgtgcc gcacaaggcc
     3361 taccaggcgc ttgcggtcgg cgtgccgctc gtctcgcgcc gctcgcgcgc catggcggag
     3421 ttcgtccgcg aggaggaaga attcctggcg gtaccggcgg gcgatgccgc ggcggtggct
     3481 gccgggatcg agcgacttgc gtccgatcct gccctgcggg cccgtgtcgg agcggccggg
     3541 cggcaggccg cggtcgatcg cgtgtcgccg gaacggatcg ggccgctcct tctcgaagcg
     3601 gtggcgacgg cgcgcgcggc gacgtcgccg aggagaaggc gatgaccggc acggccccgc
     3661 gcgcgctccg catcctccac gtggattcgg aacgcacatg gcgaggcggc gagcggcagg
     3721 tcctcgagct gatgcggcga cagcgagctc gcggcgatga gccgcatctc gccgcgccgc
     3781 gaggcagcgc gctctcgagc cgggcgtcga gcgagggatt tccgggtcac gccgttccga
     3841 tgcgcggtgc gtgggacatc ggctcggcgc tcgcgctcgc gcgtctccat cggcagctcc
     3901 gccccgacgt ggtccactgg cacgcggcgc gcgcgcacgc tctcggcgcc atcgcatccc
     3961 tctttgcacc ggggccggcg cgggtgctct cgcggcgcgt ggacttcccg gtgcgcggat
     4021 ccgcggggag ccggcttctc tacggccttc cgcgcgacgc gacgatcgcg atctcggagg
     4081 gagtccgtga cgcgctcgtc cggagcgggc tcgatccggc gtccatcgac gtggtccaga
     4141 gcgggatcga cctcaccccg ttcgaagcgc cgttcgatcg ctccgccatc cgctcccgcc
     4201 tcgggaccca ggaaggggac ttcctggttc tgcaggtagc cgcgctcgca ccgcacaagt
     4261 cgcaaaagga cctgctcgac gccgcgcgga tcgtgctcga cgagcggccg gacgcccgaa
     4321 tctgggtcgc gggagaggga atcctcaggg atgagctgga acggcagcat ggggcgctcg
     4381 atctgggcga ccgggtgcgg ctcctcggct ttcgcgacga cgttccagat ctcctccgag
     4441 cggccgatct attctgcgtt tcctcctacc tcgaggggat ggggacggcc acgctcgacg
     4501 cgatggcggt ggggctcccc gtcgtcgcga cgcgcgtggg gggcattccc gagatcgtcg
     4561 aggacggcgt gaccgggctc cttgtggcgc ctcgcgatcc ccaggcgctg gcgggggcga
     4621 tcctggcgct ggcccgggac ccggcccgca ggcgggcgat gggtgacggg gcgcgaaggc
     4681 gcgtccgggc gttctcggcc gacaggaccg aggaggagac gaggcgcgtc tatctgaagg
     4741 ccatcggggc ggtcgattga ccccgtaaat ccaagctgtt agattcgttc cgcgttcgac
     4801 ggcgacccaa ctgaggggat catgaccgaa gctctgcccg tcctgttcta cggcgccctg
     4861 ctcctgggcg ttctcgttct cgtccacgag ctgggccatt tcctcgtcgc gaaatggctc
     4921 ggtgtccgag tgatctcctt ctcgatcggc atgggtccga ggctcttcgg cttcacgaag
     4981 gacggcacgg actaccggat ctccgcactg cccctgggcg gattcgtgcg gatggccggg
     5041 gattcgcctg aagcggacga gcgcaccggc gcacccgacg agttcctctc gaagccctgg
     5101 tgggcgcgcg cgctgatcac ggccgccggc ccgggcgcca atctcgtctt cgcgctcctc
     5161 gtcaacatgg ccatcttcct gatcggcgtg gaggggcgcg actactcgac gcgcgtctcc
     5221 cgcgtgaagg cgggatcggt cgcggagagc atcgggctcc aggcgcgcga ccacatcgtc
     5281 gcgtacggcg gacatccggc gcgcacggtg tccgagctct cggacgccat cgaaaccgcc
     5341 ggcgtcgaca aatccaaatc gagccaagcg ctcccgctca cgatcgaccg gaacggcgag
     5401 acgaagacgc tcgcgctccg gcccaaggac atcgacgcgt tcgcggacgg gctcgactgg
     5461 gacatgggga cgcaggtggg gcaggtcctc atcgggtacc cggcctatga gtcgggtctc
     5521 cgcgaagggg atgagatctt gaagatcgac gggcagcgcg tcctcctctg gaccgatctc
     5581 tccacgatcc ttcgcagcaa gccgagcgcg gcgaggacgc tcctcgtgaa gcggggcgat
     5641 cagacgttcg aggtgcacct gaagaccagt cccgagggga cgatcgggat ctcgcccccg
     5701 gagacgatga cgtacaagca gacgttcccg cctatgaagg cgatcaccct cggcgtgcag
     5761 cagaccttct gggtgacggg gcagatctat tccgggctct ggggcttcgt caccgatccc
     5821 gtccgcctcc acacgacggt cgcgggtccg atcgcgatcg cgcaggtcgc gcgtcagcag
     5881 gccaagggtg gactccagca gctcgtgacc ttcgcgtcgt tcatcagcct cgcgctcatg
     5941 gccatgaacc tcctgcccat tccgatcctg gacggaggcc acatcctctt cgcgttcatc
     6001 gaggcggcgc ggcggcgccc gctctcgctc aaaacgcagc tcttcttcca gcggatcggc
     6061 ttgttcgtgc tgatgggctt ggtcgtcttc gccttctaca acgacctgag ccgcgtcgga
     6121 cagcgccggc gcgccgaagc ggacatcgac cggcggctcc agaccacatc tccgagcgac
     6181 acggccgccg cgacgcacga gccctgatcc ctctgcgggg ggccggccgc ggcatgcggg
     6241 gccgttccgc gcctctcgcg tccatccggc tctttctctt ccttgcgtcg gtcgttctgg
     6301 ccgttctctg tgccgctccg cggtcggctc gcgccgctgc cgacgacgag gccgagctct
     6361 cgttcgagcg tgagaccggc aaggtcgaga tcgtcgggaa cgagtccgtc gagaagggac
     6421 gcctgaaggg gctgctccgg acgcgctcga agtccttctg gaagccctgg acgaagcatc
     6481 cactccgcca ggactacctc cgggccgatg ccgccacgat cgagtccttc taccgccgca
     6541 ccggatatct ctacgcgcgg gtcgacagcg tcgtcgcgcg tccgacgaag gatcccaaga
     6601 aatcggacgt ggtcttctat gtgcacgaag ggccccgcgc catgatcaag gaaatcctca
     6661 tgacgggcct cggtcccatg agcgatcggg aggttcgcga ggcgctcctc taccaggtcg
     6721 ggtctccgct cgagctgggt acgctcgcgg ccagccgcga ctcgatcacc taccagtacg
     6781 gcgatcgcgg ctacgcgctc gtgagcgtct ccgactctct gcgcgtggac agcacccagg
     6841 tctcggtgct ctacgcgatc cgccccgggc cgaaggtgcg gatggggtcg atcgaggtcg
     6901 aggggacgaa caaaacgaaa ccgtcctacg tcacccggga gctcctcttc aagccagggg
     6961 acgttctcct ccgctccaag ctcatccaga gccagcagcg gatctacgac tcgggtctct
     7021 acagcgacgt ccagatggag ctggggaagg ttgacagcgt gacctcggcg agcgacatcg
     7081 cggtgacggt gcgggagcgg aagatgggat gggtcgacgc cggtatcggg tacgggacca
     7141 tcgaccagct ccgcctcacg gggcagtggg gacagcggaa catcttccgc tcgtcgatgc
     7201 gcctcgtggc gacgggccgg ctcggcgttc agatcgacac gaatccgctc cacacccacg
     7261 cgggggaccg gcggatcgac ctcgcgctca cgcatccctg gccgctcgga gtccgcgtgc
     7321 agaccacggt gggcgtctac gccgaggacg agccgatcat ccagctgacc gaccagtatc
     7381 ccgtccaggc cataggcgcc tcgctcgttc tctcgagccc catgttcaag tatggccgga
     7441 acgcggcctc gtacgagctc cggcacgtca cccaggacag cctgctcacg cagagcacga
     7501 ccaacgactc gagctacacc acgcaccgca ttgcgctcac cgcggagcgc gacacgaggc
     7561 tcaacatctt cgacccaatg aacggtagcg acgtgtacgg gcgcgtggag ttcgtcgcgg
     7621 ggacgattcc gggcaccgcc cagttcacga cgagcacgct gcaggcgacc aagtacctgc
     7681 gccggggccg cgtggtcctc gcgacgcgaa tccggggtgg cttgatcgag ccatggagac
     7741 cgtcgggccc ggacacgtcg aagaccgaag tccctctcga gctggcccag atccccaacg
     7801 aggaccgctt ccgtaccggc ggcgcgtcga ccgttcgggg ttactacgag aacgagctgg
     7861 gaacccggga agtccatacc acgacggcat cgggggactc ggtctacagc gtgatccgag
     7921 gcggccaggc gcagctcctc atttcggccg agctccgctt tcccctcctc tggatcttct
     7981 ccggcgcggc ttttttcgac ggggggaacg tctgggagcg acctgaggac atcaaaccag
     8041 aaaatatctt cagctttggg aacggggcgg ggtacaacga tatgcggtac agcgtggggc
     8101 ttggcctacg cgtcggaacc ccgatcggcc cgatccgcct ggactacggg tggaagctca
     8161 gaaccccgag gcccgacgcc cccgacgtga acccgaatcc cggcctgctc cacttcagcc
     8221 tggggcacgc ttactgacat gagccgttcc acactcgaac ctcccgtgcg cgaggcgcgc
     8281 gagcggcgcc cattccatgc gtccacggtc ttccgtgccg tgctggccgt ggttctcggc
     8341 gtgctcgcgc tggccgcgtt cgcgatcgcc tcgctcctct tcctgttccg caccggcaac
     8401 cccgagctca cgagccgcat cgttgcggag gtgaaccgga tcgccacctc ggattccacg
     8461 cgcttcacaa gctcccaggt gcgcgggacc ctcttccgcg gagcggtgat cgagaacccg
     8521 aagctcctcg tccagacccc ggacggcgaa gtggcctggg cccaagcccg gtccctgcgc
     8581 gtcgatttcg acctgatcgc gttcctgttc catcagcggc aggatctccg tatcgagctg
     8641 gacggacccc ggatcgatct cgtccacgat cgccgcgggg agctcgtgat gccccgattc
     8701 gcgcggaaga agaagggcca aggcaagggg ctcggtggaa ctcgcgtcgt gatcgcggtg
     8761 aagaacggcg ccttctcgat ggatcgcatg aagacccggt acgggaagat cggcggggtg
     8821 gcgacgctca tctccggccc cgagcactcg accatcatcc tcgaccggct ctcgggcgtg
     8881 agcgtcactc cgggacggcc cggccgtctc gagctgagcg gcgagctgga catggagaag
     8941 gcgacgctca aggcgcgtcc gctcgagatc gggctcggct cgacgcacat caccgccgag
     9001 gtggactggg atctggagaa ggcgcgtgtg cgcgagggca cgctcgacct ccacccgctc
     9061 gagctgcgcg agttcttcaa gtccctcgag atcgcgaccg aggacggcac ggcgcggggt
     9121 gaggtgacct tctcgggcac gccgacggat ggcaaggcgc gcgtctgtct cgcaggcacg
     9181 ttcgccggcg agccggtcga cacgctcgtg ctcgaggcgc gatcgcgtcc tggagcggtc
     9241 gacttctccg gattgaatct ccgggtgcgc tccacgaacg tgaccggcgg cggcacgttc
     9301 gacacgcgcg gcgtgctgaa cacggacctc gcattccgga acgtgaaccc gaaggtgctc
     9361 ccctggtgga agtcgccgga gaacacgccg gacggatcgc tggccggccg cggccgcttc
     9421 acggtgcgcc gcgcggcgcc caacatcgac gtcctcgcct cggtcgacct catgccgagc
     9481 cggctcggca agctccagat ctcgcgcgga cacgtgcgcg tcgcgaccca tccgaacggc
     9541 tccgcgcagc tcgactcgag ctggatcgag atccccggca gccggatcgt ggcggcggga
     9601 acgctgggca aggacggcgc gctggccgcg acggcgcacg ccgccgtgca caacctcgag
     9661 gggatgaacg tgctcctggc ggctccgcag atcgaggcgg ggcacggccg cgtggacgct
     9721 gccttcagcg gaacgatcga ggctccgcaa taccaggccc agatggatct cttcgacacg
     9781 cgcctcaaga acggaatcgg aggcgattcg ctcacggtcg aggtgcaggg caagctcaag
     9841 cccgagctcg atctcatcgc cgacgccggc attcgcgggc tcaaagccgg cacgcgcccg
     9901 ctcggcaacg tggtcgcgac cgtgagcggc gggaagacgc tccggatcga gcgataccgc
     9961 caggcgctcg gcgacacgct catcacgctc ggcgggaagg tgatctttgc ggagcaggga
    10021 gcggaagctc tggtcgactc ggtcgcgctc acggcgggcg agtaccacgt ccggagccgg
    10081 gggccggtca aggtcggctt cttcgggagc cgcgtacgcg tgtccgatct cgccctcgac
    10141 ctcgatccag gcacgatgca cctcgatctc gactacaacc cgtcgggcga cgcgatcgat
    10201 gcgcgcgggt ccatcgcgga cgtcgacctc ggccgcatcc ccgggctcgc gcatggcgcg
    10261 atccgctatg ccggagtcgt ccacggcgac tttctcgcaa ccgggccact caaagatccg
    10321 gacgtctcgc tccggctcgc ggtcgtggag ccgacggtcg gagacatcgt gggggacagc
    10381 gtgcgcgtcg atctcgacta cgcgcccggc gtgctcaccg tggagcaggc cgcctggacg
    10441 agccaggcga gccgcctcgc ggccagcgga acgatccggc cccaccttgc gcttcaagag
    10501 tggctcaagg cgatcgcgaa aggggaccgg agctggtcca agcgcgcgga tctcgcgctc
    10561 acggcgtccc tcgattcctt cgatctcaag ctcgtggcgc cggccgacac gtcgctccgc
    10621 tcgctccaag ggatcgcgac cggcaaggcg cgcatcagcg gcacgacctc ggacccggtg
    10681 atgggcgtcg aggggcgcgt ctcgtcgatg tcctatcgcg gcgtccaggg cgagatcgcc
    10741 ggcgtcgacc tcgcctacga gaaccgcagg ctcaagatca accggttcga cgtgaagcag
    10801 aacgagtcga tctcccaggt gcgcggggag cttccgctcg atctcggcat ctacgccgag
    10861 gaccgcgtga tggacaagga gcccatgacc ctcaccgtcg acatcccgaa cggtgacttt
    10921 tccatcgccc gcattctcct gcccgatctg atcgggtaca gcaccggcca ctacaccgcg
    10981 tccgcggtcg tatcggggac gccctcggac gcgcgcgtga cggggtcgtt caagctcacg
    11041 ggcgcgagga tccggcccgc gggccgggac gaggttctga ccaatgtcga aagcgaggcc
    11101 acgttcgacc agaagcagct cacggtcacg aagctgacgg cgaagcaggg agagaagggg
    11161 aagctcgagg ccaacgggct ctggcggtgg cccacgggcg ccaaggcgga gaagccggcc
    11221 ttcggaccgc cggggacgta cgagttccat gtgatcggga ccgacttcac cgcgacggac
    11281 cggtcgacct acgccgtgcg cttcaccggg aagatcgaca tcgtgaacgg aaaggatccc
    11341 ttgggcggtc agcgtccact catcacggga cacggcaccg tcacgaaggg ggagctgaca
    11401 ctcgacctct cgaagccccc ctccacgccg tcggagccgc ttccgttcct ctacagcgtg
    11461 aacgtcgacc tgcccgggaa tttcttctac cgcaacctcg acgccgaggt cgagctggag
    11521 acggtcgatg gtcccctctc tttcaagaac gaaggcgatg gcgatctcgc gctcgggacc
    11581 ctcgaagtgc gcgggggcaa gtactacgtc atgacccgcg agttccgaaa tctccagggc
    11641 acgatcagtt tcaacaaccc agatcgcatc gacccggacg tgaacgtcgt gggcgagacc
    11701 cagatccccg atcccagctc gacggagggc tcgcgcaccg tgtacctcgc gctgaccgac
    11761 cgggtgtcgc ggctcaaggt ccgcgtgtac gacgacgcgg gaactccgcc gaacgacctc
    11821 tggaaggcgc tcgcctttgg acagttcgtt cccatgacgg gcgtctcgac cgaagccgcc
    11881 gcgaccggtg gcgtggaccc atcggttgcc gtgcccatct cgaactatct cttccagaac
    11941 gtggagcgct ggatcgggag ctcgggcttc atcgacacga tcgatctgcg cgggagcggc
    12001 ggtgccgccg acggcagcaa gtcgacgagc gcgctcagta tggtgggcgt gggaaagtac
    12061 gtgaccccgc agttctactt caagtacagt aaggacttct cagccaaggc ggagcaggag
    12121 atcagcgcgg actaccgcgt gacccggcac ctcctgctga agggccagca gatccggagc
    12181 acggacccgg cccggccgcc ccaggaatac aatctcgacc tcaagatccg actggaatac
    12241 tgatcgacgc gtggaactga atccggtcca gcgccaggcc gtggagcatc ccggtgggcc
    12301 cctcctcatt ctcgcgggcg cggggagcgg gaagacgcgc gtcctgaccg ggcgggtggc
    12361 gcacctcgtg cgcgagcgcg gcgtccacga ggggtcgatc ctcgccttca cgttcacgaa
    12421 caaggcggcc cgcgagatgc gcgagcgggt ggaatcgctc ctcgaaacgg acgagctccg
    12481 gacctgggtc ggcaccttcc acgccacctg cgtgcgcatt ctccgccgcc acgcggagct
    12541 cctcggcttc ccgaagtcct tcgtcatcta cgataccgac gaccagcgca cgctcctccg
    12601 cgagctcctg cgcgacgagg gaggagatga ccgcacgctc acgcccgccg gcgcctcgtc
    12661 ccggatctcg cggctcaaga acgagggcgt gacgccggag gcgttcgaat cgcagatccg
    12721 ctcgcccatc gaccggaagc tcgcgcccgt gtacgcgaag tacacgaaag cgctccgcga
    12781 ccgggcggcg atggatttcg acgacctgct tcttttcacg gtccggctct tcgacgcgga
    12841 cgaggacgtc caccgttcgt acgcggggcg cttccagcac gtcctcgtgg acgagtacca
    12901 ggacacgaac tcgatccagt tcgagctgat cgagcggctc tcgcgcgtgc accggaacct
    12961 caccgtggtg ggggacgacg atcagtcgat ctaccgctgg cgcggggccg acgtggggaa
    13021 catcctctcc ttcgagaagc agttcccgga cgcaacggtg cttcgcctca cgcagaatta
    13081 ccgctccacc cggaccatcc tccgcgcggc gaacggcgtg gtgaagcaca acgcggggcg
    13141 gaaggagaag gagctctgga ccgagaacga ggcgggcaat ccgctcaccc tccacgtcct
    13201 tcccgacgag gaggccgagg gagagaaggt ggtctcgctc ctcctcgagg cgcgccgacg
    13261 cgatgaccgc tcgaacggag acttcgtcgt gctctatcgc accaacgcgc agtcgcgagc
    13321 ggtggagaac gcgctccgcc gctcggcgat cccgtaccag atcacggggg gcatctcgtt
    13381 ctacgagcgc cgcgaagtga aggacgtgct cgcgtatctg cgggcgacgg ttcagccgcg
    13441 ggacacggtc gcgtggcttc gcgtgatcaa cgtccccggg cgcggcatcg ggaagaccac
    13501 ggtggaccgc ctgcgcgagt tcatggacgc gcgcggcctc acggttcccg aggcgctcgc
    13561 gcacccggag ctcgtgcagg cggtcggtcc gacgtcgtcc cggaagcttc tcgaggtcgc
    13621 gcatctcttc gagtccttga agccgctcct cacgctctcg cctcccgcct gtgtgacgga
    13681 gctcgtcgcg cgggtgcggt accgcgagta cctgcaggaa gaggagcccg ccgaggcggc
    13741 ggagcggatc gagaacgtcg aggagctgat cgcgggcgcg gaggcctacg cgcgccgcgt
    13801 ggaagagccg agcgtcgagg gatttctcgc c
//
LOCUS       DASTKC010000189         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_393625, whole genome shotgun sequence.
ACCESSION   DASTKC010000189 DASTKC010000000
VERSION     DASTKC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393625"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VFR25_07800"
     CDS             complement(<1..1603)
                     /locus_tag="VFR25_07800"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9940991.1"
                     /translation="MTALSDPIRYLEGVGPEMARRLARLEIRTVKDLLFHIPVGYRDR
                     REVTPIAKLTPGAEVSIEASVASVRPLRRARGRRDLEATLKDSSGMLRAVWFHQPYLA
                     STLKAGERYIFSGTVHAFRGPELHNPEFEPAGGEGNHLHVARIAPRYALTEGITERWL
                     RARVRAALDRLKPPPEVLSGEWRKRLELPPLPVAFERIHFPEDVAEREPARKRLALEE
                     ILALQVALQFVRRRHRKAQRATPLAAGDAAMRRYLAALPFPLTSAQGRSLDAMVKDLD
                     NEVPMRRLLLGDVGSGKTVLALAGAVRAAGAGQQAALLAPTMLLAEQHASTASKQLEA
                     AGVTWRLLTAATPERERRQIQDGLASGEISIAIGTHALLEPKVAFRSLAFVAVDEQHR
                     FGVRQRIALARGTDPGRSAHLLVLTATPIPRSYAMTLYGDLDLSILDEKPPGRLPVTT
                     TSIDGTRREALLDLLRGEMERGGSAFVVYPIVEESEKVDLEDATGTAAALRKEPALAQ
                     AGIELVHGRLPAEERRRALERFRGGD"
     gene            1710..1934
                     /gene="rpmB"
                     /locus_tag="VFR25_07805"
     CDS             1710..1934
                     /gene="rpmB"
                     /locus_tag="VFR25_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET9940992.1"
                     /translation="MARTCDICGKGVQHGQNVSHAHNVTKRRWEVNLQTVRALVSGKP
                     KRIRVCTRCIRTGSVQKHMGAKAAQRARAI"
     gene            complement(2001..3248)
                     /gene="ftsZ"
                     /locus_tag="VFR25_07810"
     CDS             complement(2001..3248)
                     /gene="ftsZ"
                     /locus_tag="VFR25_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9940993.1"
                     /translation="MFELVEDGKVVLGASIKVIGVGGAGGNAVNRMMEAGLRGVDFIA
                     ANTDAQVLDQSRCPKRIQLGTGITKGLGSGANPNVGREAAEEDEAAIAEALGGADMVF
                     VTAGMGGGTGTGAAPVVARVARSMGALTVAVVTRPFEFEGRRRMQIAEEGLQALREKV
                     DTLIVIPNQRLLAIVEKHTPLKEAFRVADQVLHYATKGISDLITVPGLVNLDFADVKT
                     VMAERGNALMGAGHSSGPNRAYEAAQCAVSSPLLDDVSISGAEALLVNVTGGESMTLH
                     EINEAVTVVVDAAGRDANVIFGAVIDDSMGDALSITVIATGFGKAEMMKARAAEPARA
                     HAPRILEMEPRPVRPIVVRPTLEDMDAEETVLEASSSSDAQAPAGRPQFKMTPGTARR
                     PFGGRAISKENMDVPAFMRKQMD"
     gene            complement(3315..4547)
                     /gene="ftsA"
                     /locus_tag="VFR25_07815"
     CDS             complement(3315..4547)
                     /gene="ftsA"
                     /locus_tag="VFR25_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9940994.1"
                     /translation="MADGPIYAGLDIGTTKITAVVAEPDPSGEGIRILGIGSAPSDGL
                     KRGVVVNLERTVRSIQHAVQEAERIAGRSVKSVSAGIAGDHIRGINSRGVIAVSRKDS
                     EIRAVDVARVVEAAKAVAIPADREILHVLPQQYVVDDQDGIRDPTGMSGVRLEAEVHI
                     ITGAASACRNVLRAAERAGLQVDELVLEPLASATAVLRPDEKDLGVAILDIGGGTTDI
                     AVFYEGSVRHTAVIGLGGANVTNDLAIGLRTPVERAELLKLEAGCALTSMVRPDETVR
                     VPSVGGRPDRDISRHMLAMMIEPRVEEIFAHASKEIRKHHMGAELLGGGVVLTGGASL
                     LAGMTELAEQVFDLPVRRGAPMGFTGHEETVSDPRYATGVGLAMHAYQNDGGRRVADG
                     NWMGRIGIGIRRWIEELV"
     gene            complement(4558..5394)
                     /locus_tag="VFR25_07820"
     CDS             complement(4558..5394)
                     /locus_tag="VFR25_07820"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9940995.1"
                     /translation="MRAGYAGLPTYKRPARKRRNRRILLLGAVLLLVTLAAPFLTAPC
                     ARALGLIPLFRAERIEVTGLLYLSPDEVRSWIPVRERENLLLIHLDEVASALALHPRI
                     AGARVSRHPGRLRIDVTERRTFLLVNAGTLLEVDQSGTILSPLDRGLVADRPVLSGVP
                     FPTVKPGARVTTARLRDILHLVEVLESPEVGLVSDVSEVVSEDPHRAILRLSRDRIPV
                     TVDPERATVASMRALSAALRDLRERERHVLSVDARYRGQVVVRCAPEDSVSAALPPRG
                     TT"
     gene            complement(5391..>6029)
                     /locus_tag="VFR25_07825"
     CDS             complement(5391..>6029)
                     /locus_tag="VFR25_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9940996.1"
                     /translation="RGHDLEVGRRGSRFRVVIRGEEQGWIDLQVAGVHNARNALAAAA
                     AAWELSVPFAIIQAGLRDFAGVGRRLESRGQVKGAVWVDDYAHHPTEIEAALGALKTS
                     YGRPITAVFQPHRYSRTQALLDRFATCFQGVETLVLLPIYPAGETPIDGVSSERLAKA
                     VEAAHGPRVILAHSFPEAAEAAAQALRPEGLLATIGAGDVYRIGELIRGGGR"
BASE COUNT          900 a         2180 c         2042 g          907 t
ORIGIN      
        1 cgtccccgcc ccggaaccgc tcgagcgcgc ggcggcgctc ctccgcgggc agacggccgt
       61 ggaccagctc gattccggct tgggcgagcg cgggctcctt cctaagcgcg gccgcggtgc
      121 cggtcgcgtc ctccagatcc accttctcgc tctcctcgac gatgggatac accacgaacg
      181 cgcttccgcc ccgctccatc tctccgcgga ggagatcgag gagcgcctcc cggcgcgttc
      241 cgtcgatcga ggtcgtcgta accggcaggc ggccgggcgg cttctcgtcg aggatggaga
      301 ggtcgagatc gccatagagc gtcatggcgt agctccgcgg aatcggcgtc gcggtgagga
      361 cgaggagatg cgcgctccgt ccggggtccg tgccccgcgc gagcgcgatc ctctgccgca
      421 cgccgaagcg gtgctgctcg tcgacggcga cgaacgcgag cgagcggaag gcgaccttgg
      481 gctcgaggag cgcgtgcgtc ccgatcgcga tcgagatctc ccccgaggcg agaccgtcct
      541 ggatctgccg ccgctcccgc tccggagtcg ccgcggtgag aagcctccac gtgacgccgg
      601 cggcttcgag ctgcttcgat gcggtcgacg cgtgctgctc ggccaggagc atcgtggggg
      661 cgaggagcgc cgcctgttgc cccgcgcctg cggcgcgcac ggcccctgcg agcgcgagca
      721 cggtcttgcc ggagccgaca tcgccgagga ggaggcggcg catcggcacc tcgttgtcga
      781 gatccttcac catcgcgtcg agcgatcgcc cctgcgcgct cgtgagcgga aagggaagcg
      841 ccgcgaggta gcgccgcatg gcggcgtcac ccgcggcgag cggcgtggca cgctgcgcct
      901 tccggtgccg gcgccgcacg aactggagcg cgacctggag cgcgagaatc tcctcgagcg
      961 cgagccgctt ccgcgcgggc tcccgctcgg cgacatcctc cgggaagtga atccgctcga
     1021 acgcgaccgg caagggaggg agctcgagtc gcttcctcca ctcgcctgag agcacctcgg
     1081 ggggcggctt cagtcgatcc agggccgcgc ggacccgcgc gcgaagccac cgttccgtga
     1141 ttccctccgt gagcgcgtag cggggcgcga tccgggcgac gtggaggtgg ttcccttccc
     1201 ctccggccgg ctcgaactcc ggattgtgga gctcgggacc acggaaggcg tgcacggtcc
     1261 cggagaagat gtagcgctcc ccggccttga gggtggacgc gaggtagggc tggtggaacc
     1321 agaccgcgcg gagcattccg ctcgagtcct tgagcgtggc ctcgaggtcg cggcgaccgc
     1381 gcgcgcggcg gagcggacgc acggaagcga ccgaagcctc gatcgatacc tcggcgcctg
     1441 gggtcagctt cgcgatcggg gtcacctcgc ggcggtcccg atagccgacc ggaatgtgga
     1501 agaggaggtc cttgacggtt cggatctcga ggcgcgccag gcggcgcgcc atttccggcc
     1561 cgaccccctc gaggtagcga atcggatcgg acagggcggt cacgggggcg atgctagggg
     1621 ggtgaggcgg tcaagtcaaa ccaaggcggg ggggttgcac ccggcggcaa gggtcggtac
     1681 aatgcccggc tcacccacgg aggtgtttca tggccaggac ttgcgacatt tgcggcaagg
     1741 gcgtccagca cggtcagaac gtgagccacg cccacaacgt gacgaagcgc cgctgggagg
     1801 tcaatctcca gacggttcgc gcgctcgtca gcggcaagcc gaagcggatt cgcgtttgca
     1861 cccggtgcat ccggaccggc tcggtccaga agcacatggg ggccaaagcg gcgcagcgcg
     1921 cccgggcgat ctagacccgg acgcgctgcc gcaccgcttc atcgccccgg ctcccgccgg
     1981 ggctttttcg tttccctgcc tcagtccatc tgcttccgca tgaacgcggg aacgtccatg
     2041 ttctccttgc tgatcgcccg cccgccgaac ggccggcgcg ccgtgcccgg cgtcatcttg
     2101 aactggggac gaccggccgg agcctgagcg tcgctcgaag acgaagcctc gaggaccgtc
     2161 tcctccgcat ccatgtcctc cagcgtgggg cgcacgacga taggacgcac cggacgcggc
     2221 tccatctcga gaatccgcgg cgcgtgggcc cgagcgggct ccgcggcgcg ggccttcatc
     2281 atctcggcct tgccgaagcc ggtcgcgatg acggtgatgg agagcgcgtc tcccatcgaa
     2341 tcgtcgatca ccgcgccgaa gatcacgttc gcgtcccgtc ccgcggcgtc cacgaccacg
     2401 gtgaccgcct cgttgatctc gtgcagcgtc atcgactcgc cgccggtgac gttcacgagg
     2461 agcgcctcgg cccctgagat ggacacgtcg tcgagcagcg ggctcgagac cgcgcactgc
     2521 gccgcctcgt acgcccggtt cggaccgctc gagtgtccgg ctcccatgag cgcgttcccc
     2581 cgctccgcca tgaccgtctt cacgtcggcg aagtcgaggt tgacgagccc gggcaccgtg
     2641 atgagatcgg agattccctt cgtggcgtag tggagcacct ggtccgcgac gcggaaggcc
     2701 tccttcagcg gcgtgtgctt ctcgacgatc gcgaggagcc gctgattcgg gatcacgatc
     2761 agcgtgtcca ccttctcgcg aagggcctgg agcccttctt ccgcgatctg catgcggcgg
     2821 cggccttcga actcgaacgg acgcgtcacg accgcgaccg tgagcgctcc catcgagcgc
     2881 gcgacgcgcg ccacgaccgg cgccgcgccg gtcccggtgc cgcctcccat gccggcggtc
     2941 acgaacacca tgtcggcgcc accgagcgcc tcggcgatcg ccgcctcgtc ttcctcggcc
     3001 gcctcgcgtc cgacgttcgg attcgcgccg gagccgagcc ccttcgtgat cccggtgccg
     3061 agctggatcc gcttcggaca gcgggactga tccagaacct gtgcgtcggt gttcgcggcg
     3121 atgaagtcga cgccgcgaag acccgcctcc atcatccggt tgaccgcgtt ccccccggcg
     3181 ccgccgacgc cgatgacttt gatcgaggct cccaacacga ccttcccatc ctccaccagc
     3241 tcgaacatgt cactcctccc tgaacagcgg ttgcgccggt cttcccaaac cagctcttgc
     3301 gaaacctgac cctgctacac cagctcttcg atccagcggc gaatcccgat accgatcctc
     3361 cccatccagt tgccgtctgc gacacggcgg ccgccgtcgt tctggtaggc atgcatcgcc
     3421 agcccgacgc cggtcgcgta tcgaggatcc gaaaccgtct cctcgtggcc cgtgaatccc
     3481 atcggggcgc cgcgccttac cggcaggtcg aacacctgct cggccagctc cgtcatcccc
     3541 gcaaggagcg aggcgcctcc cgtcagcacc acaccgccgc ccaggagctc ggcgcccatg
     3601 tggtgctttc gaatctcctt gctcgcgtgc gcgaagatct cctccacgcg cggctcgatc
     3661 atcatcgcga gcatgtgccg cgagatgtcg cggtcgggac gcccgcccac gctcggcacg
     3721 cgcaccgtct cgtccggccg caccatcgac gtgagcgcgc atcccgcctc gagcttcaag
     3781 agctccgcgc gctcgaccgg agttctgagt ccgatcgcga gatcgttcgt cacgttggcg
     3841 ccgccgagtc cgatgaccgc agtgtgcctc acggagcctt catagaaaac agcaatatcg
     3901 gttgttccgc ctccaatatc caaaattgcg acgccgaggt ccttctcatc gggtcgaagc
     3961 accgcggtcg cggacgccag aggctcgagc acgagctcgt ccacctggag tcccgcgcgc
     4021 tcggcggcgc ggagcacgtt ccggcatgcc gaggcagccc cggtgatgat gtgcacctcg
     4081 gcctcgagac ggactccgga catgccggtg gggtcgcgga tcccgtcctg gtcgtcgacg
     4141 acgtactgct gcgggagcac gtggaggatc tcgcggtccg ccgggatcgc gaccgccttc
     4201 gcggcctcga ccacgcgcgc cacgtccacg gcgcggatct ccgaatcctt cctcgagacc
     4261 gcgatcacgc cgcggctgtt gatgccgcgg atgtggtccc ccgcgatgcc cgccgagacg
     4321 ctcttcacgc tccgccccgc gatccgctcg gcctcctgca cggcgtgctg gatggagcgc
     4381 accgtcctct ctagatttac gaccacgccc cgcttcaatc cgtcggacgg ggcgcttccg
     4441 atgcccagga tccggattcc ctccccggat gggtccggct ccgccacgac cgccgtgatc
     4501 ttggtggtcc cgatgtcgag tcccgcgtaa ataggtccgt ccgccatgtc cgccccccta
     4561 ggtggttccg cgcggcggca gggccgcgct cacgctgtct tcgggcgcgc aacgcaccac
     4621 gacctgtccc cgataccgcg cgtcgacgga gagcacgtgc cgctcccgct cgcggaggtc
     4681 ccggagcgcg gcggagagcg cccgcatgga cgcgaccgtc gcacgctccg ggtcgaccgt
     4741 gaccgggatc cggtcccgcg agagacgcag gatcgcccgg tgcggatctt ccgacacgac
     4801 ctcggacaca tcggacacga gccccacctc gggcgactcg agaacctcca ccagatggag
     4861 gatgtcccgg aggcgcgccg tcgtcacccg cgctcccggc ttcacggtcg ggaacggaac
     4921 gccggagagg acgggacggt cggcgaccag cccgcgatcg agcggcgaga ggatcgttcc
     4981 cgactggtcc acttcgagga gcgtgcccgc gttcacgaga aggaacgtgc gccgctccgt
     5041 gacgtcgatc cggagccgcc ccggatggcg cgagacgcgc gcgccggcga tccgcggatg
     5101 gagcgcgagc gcggacgcga cctcgtctaa gtggatgagg aggaggtttt cgcgctcccg
     5161 cacgggaatc caggagcgga cctcgtccgg cgacagatag aggagccccg tcacctcgat
     5221 ccgctccgcg cggaacagcg gaatcagccc gagcgcccgc gcgcacggag ccgtgaggaa
     5281 gggcgccgcg agcgtgacca ggagaagcac ggcgccgagg aggaggatgc ggcggttcct
     5341 ccgcttgcgc gccggacgct tgtaggtcgg aagtcccgca taccccgctc tcatcgccca
     5401 cctccccgga tcagctcgcc aattcgatac acgtcgccgg ccccgatcgt ggcgaggagt
     5461 ccctcggggc ggagcgcctg ggccgcggct tccgcggctt cggggaacga gtgcgcgagg
     5521 atcacgcgtg ggccgtgggc cgcttccacc gccttcgcga gacgctccga cgacacgccg
     5581 tcgatcgggg tctcccccgc cggatagatg ggcaggagga ccagcgtctc cacgccctgg
     5641 aagcacgtcg cgaaccggtc gaggagcgcc tgcgtgcgcg agtagcgatg cggctggaag
     5701 acggcggtga tcggccgtcc gtaggaggtc ttgagcgcac cgagcgcggc ctcgatctcc
     5761 gtcgggtggt gcgcgtagtc gtcgacccag acggcgccct tcacctggcc gcgggactcg
     5821 aggcggcgcc ccactcccgc gaagtcccgg aggccggcct ggatgatcgc gaacggcacc
     5881 gagagctccc acgccgcggc cgccgcggcg agcgcattcc gcgcgttgtg cacgcccgcg
     5941 acctggaggt cgatccaccc ctgctcctcg ccgcggatga cgacccgaaa gcgcgagccg
     6001 cggcgtccga cttcgaggtc gtggccgcg
//
LOCUS       DASTKC010000190         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1577262, whole genome shotgun sequence.
ACCESSION   DASTKC010000190 DASTKC010000000
VERSION     DASTKC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577262"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="VFR25_07830"
     CDS             complement(<1..1557)
                     /locus_tag="VFR25_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9940997.1"
                     /translation="MMQSLRDNMKLVIWITAIAFLVGFGILQLGGVLDNNPMQRHSGV
                     IARINGEPVRLEEYNRIVQQMTQQLTSQRPLQEGEDSYIREQAWQQIVSMKLMEQQAK
                     AHHVDVTPEEIKSAIRLTPPDFVMKAPIFLTDGQFDYRKYLAEMDNPNSQVPWAQVEA
                     IVASQLPAQKLQTDVIQGAKVSDGDVRDRFLLQNEKLNIHFVAFDPDSFPIDTSRVGG
                     ADVENYYKAHPEKFTGPKQVKIQVVLIPRVPNDSDFAAARERLQGILEQVRALPDSFP
                     MYARTYSEIGSASAGGDPKGEPYFEEMRPTFRNGLRNVIAGQISPIIKEERSLHIFRV
                     DRRYPDPVTGRERIKYHEIAVRVQPGADAIRATRTQIADIEKEAKGQGLATVATKRGL
                     RTFQSQYFAEGMSQNSIFDRFPEIEQWCFNARINSISHPIPTEAGWYLYQILDRREPG
                     LRSLQQVEVEAKEALIRSLKEARAQDVAKQALAAVQAGAKPEDVAKQYRGLADTAVDI
                     TRNGAMGRLGM"
     gene            1765..3009
                     /locus_tag="VFR25_07835"
     CDS             1765..3009
                     /locus_tag="VFR25_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9940998.1"
                     /translation="MPRVEAPDIERDLLGRVIDALDTGLLALDEDRHIAAINDTLAKG
                     WGVERAVVVGQPISAVFESETERWYLPVRGAEARATREVRGTVAEREVLMRYSATGLG
                     DSGDVLIRVEDLVDADTEEEVFRNTERLISLGELSARVAHEIRNPLTGVRTTVQFVAS
                     KLRAGDSRRDDLQDVLKELDRIEQIITDLLLFARPQAARPVPTDFREIVEKVLDILAR
                     RFMDGGIEVDVDLEADVPLVQVDPDMVQQVVLNLVINAIQAMPNGGVLRVTTGLRRTR
                     YKKAYVDLTIADTGTGIPEEVKEKIFDPFYTTRSMGTGLGLSISLQIAREHGGNVTAR
                     NLAQGGAAFRFALPAMLVPEEVTANGGPEVKAVEKVAERAEAKSVEPRERSEAKTEPR
                     EPKAAEPREAKAETREKVEEKA"
     gene            3006..>3567
                     /locus_tag="VFR25_07840"
     CDS             3006..>3567
                     /locus_tag="VFR25_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9940999.1"
                     /translation="MKLRIRIADDEELIRKSLTKLLRSEDYDVDAVGTAAEVLESVRR
                     DPPHVLVLDLRLPDGSGLDLLPRLKSMAPEMKVVVITAFGDLPTAVEAMRQGATDFLK
                     KPYEMHEMLLAVERLKAGLVRETQLDAFRRGELETFLKTRIVGESPAMRRIWDIVEKV
                     AGSEATTVLIEGESGTGKELVARAVHF"
BASE COUNT          572 a         1153 c         1260 g          582 t
ORIGIN      
        1 cataccaagc cggcccatgg cgccgttcct ggtgatgtcc acagcggtat cggcgaggcc
       61 gcggtactgc ttcgcgacgt cctccggctt ggcgccggcc tggaccgcgg cgagggcctg
      121 cttcgcgacg tcctgcgcgc gcgcctcctt gagcgaccgg atgagcgctt ccttggcctc
      181 aacctcgacc tgctggagcg agcggagccc gggctcgcgg cgatcgagga tctggtagag
      241 gtaccagccg gcctcggtcg ggatcgggtg cgagatcgag ttgatccggg cgttgaagca
      301 ccactgctcg atctcgggga agcggtcgaa aatgctgttc tggctcatgc cttccgcgaa
      361 gtactgcgac tggaaggtcc ggagcccgcg cttggtcgcg accgtggcca gcccctggcc
      421 cttcgcctct ttttcgatgt ctgcgatctg agtccgggtc gcgcggatcg cgtcggcgcc
      481 gggctgcacg cgcaccgcga tctcgtggta cttgatccgc tcccgcccgg tcaccggatc
      541 ggggtagcgg cgatcgacgc ggaagatgtg gagcgaccgc tcctccttga tgatcggcga
      601 gatctggccg gcgatcacgt tccggaggcc gttgcggaag gtcgggcgca tctcctcgaa
      661 gtagggctcg cccttcggat cgccgcccgc ggacgcgctt ccgatctcgg agtaggtgcg
      721 ggcatacatc gggaacgaat ccgggagggc gcgaacctgc tcgaggattc cctggagccg
      781 ctcgcgggcc gccgcgaagt ccgaatcgtt cggcacgcgg ggaatgagga ccacctggat
      841 cttgacctgc ttcgggccgg tgaacttctc cgggtgagcc ttgtagtagt tctccacgtc
      901 cgccccgccg acccgcgagg tgtcgatcgg gaagctgtcc gggtcgaagg cgacgaagtg
      961 gatgttgagc ttctcgttct ggaggaggaa gcgatcccgc acgtcgccgt ccgagacctt
     1021 ggcgccctgg atgacgtcgg tctggagctt ctgcgccggg agctgggagg cgacgatggc
     1081 ctcgacctgc gcccacggga cctgggagtt ggggttgtcc atctccgcga ggtatttccg
     1141 gtaatcgaac tggccgtccg tgaggaagat cggcgccttc atcacgaaat ccggcggcgt
     1201 gaggcggatc gcgctcttga tctcctcggg cgtgacgtcg acgtggtggg ctttggcctg
     1261 ctgctccatg agcttcatcg acacgatctg ctgccacgcc tgctcgcgga tgtagctgtc
     1321 ctcgccctcc tggagggggc gctgggacgt gagctgctgg gtcatctgct ggacgatccg
     1381 gttgtactcc tcgagccgga cgggctcgcc gttgatccgg gcgatgaccc ccgagtggcg
     1441 ctgcatcggg ttgttgtcga ggacgcctcc gagctgaagg atcccgaacc cgaccaggaa
     1501 ggcgatggcc gtgatccaga tgaccaattt catgttgtcg cgcaacgact gcatcatggc
     1561 cggaacacct cgatgggatg tggggtggag agggccgcgg agggagccga gagcctccaa
     1621 aaaatccgga taaggctacc agcggcgctc cctcgaagca aggacgggct ccggcgccgg
     1681 tttgtgggtc gttttgagtt gacggcccac ggcacgcgta gcagggtgtg ctcatgaggt
     1741 ccgttcagga tcggagcgtt cgtcatgccg cgtgttgaag caccggatat cgagcgagac
     1801 ctcctcgggc gcgtcatcga cgcgctcgat acggggctcc tcgccctcga cgaggaccgc
     1861 cacatcgccg ccatcaacga caccctcgcc aaggggtggg gcgtggagcg cgcggtggtc
     1921 gtgggccagc cgatctccgc cgtgttcgaa tccgagacgg agcgctggta cctcccggtg
     1981 cgcggcgccg aggcgcgggc cacccgcgag gtccggggca cggtggccga gcgcgaggtc
     2041 ctcatgcgct acagcgcgac cggcctcggc gactcgggcg acgtcctgat ccgggtcgag
     2101 gacctggtcg acgcggacac cgaagaagag gtattccgga acaccgagcg cctcatctcc
     2161 ctgggcgagc tctccgcccg cgtcgcccac gagatccgga atcccctgac cggcgtccgg
     2221 accacggtcc agttcgtcgc gtccaagctc cgcgccggcg actcgcgtcg ggacgacctc
     2281 caggacgtcc tcaaggagct cgaccgcatc gagcagatca tcaccgacct ccttctcttc
     2341 gctcgacccc aggcggcccg cccggttccg accgacttcc gcgagatcgt ggaaaaggtc
     2401 ctcgacatcc tcgcccgccg gttcatggac ggtgggatcg aggtggacgt ggatctagag
     2461 gcggacgtcc ccctggtgca ggtcgacccc gacatggtcc agcaggtggt gctgaatctc
     2521 gtgatcaacg cgatccaggc gatgccgaac gggggcgtcc tgcgggtgac gacgggcctg
     2581 cggcggacgc gctacaagaa ggcctacgtc gacctcacga tcgccgacac cggcaccggg
     2641 attccggagg aggtgaagga gaagatcttc gatcccttct acacgacccg gtcgatgggg
     2701 accggactcg ggctctcgat ctcgctccag atcgcccgcg agcacggcgg gaacgtcacc
     2761 gcgaggaacc tcgcgcaggg aggcgcggcg ttccgtttcg ccctcccggc gatgctggtg
     2821 ccggaggaag tgacggcgaa cggagggcct gaggtgaagg cggtggagaa ggtcgcggag
     2881 cgtgccgagg ctaagagcgt ggagccgcgc gagcgctcgg aggccaagac cgagccgcgc
     2941 gagcccaagg ccgccgagcc gcgcgaggcc aaggccgaga cccgcgagaa ggtggaggag
     3001 aaggcgtgaa gctccggatc cggatcgccg acgacgaaga gctgatccgg aagtccctca
     3061 ccaagctcct gcgcagcgag gactacgacg tggacgcggt cggcaccgcg gccgaggtgc
     3121 tcgagtcggt ccggcgcgac ccgccacacg tcctggtcct cgatctccgg ctccccgacg
     3181 gcagcggcct cgatctcctt ccgcgcctca agagcatggc ccccgagatg aaggtcgtgg
     3241 tcatcaccgc cttcggcgac ctgccgaccg ccgtcgaggc gatgcggcag ggcgcgaccg
     3301 acttcctcaa gaagccgtac gagatgcacg agatgctcct cgcggtggag cggctcaagg
     3361 cggggctcgt ccgcgagacc cagctcgacg ccttccgccg tggcgagctc gagaccttcc
     3421 tcaagaccag gatcgtcggc gagtcgccgg cgatgcggcg gatctgggac atcgtcgaga
     3481 aggtcgcggg gagcgaggcg accaccgtcc tcatcgaggg ggagagcggg accggcaagg
     3541 agctggtcgc gcgggcggtc catttcg
//
LOCUS       DASTKC010000191         8477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1104903, whole genome shotgun sequence.
ACCESSION   DASTKC010000191 DASTKC010000000
VERSION     DASTKC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104903"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFR25_07845"
     CDS             <1..840
                     /locus_tag="VFR25_07845"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941000.1"
                     /translation="DALFQIAKSSYQDKKYAEAADGFRRVVSQFPGYSAADDAQFLLA
                     DSYAKAGSAEQSRLAYEQFLAFFPSSELRPTAEFQLGLQYFQGQDFMHAAVSFTQVLG
                     ESATTDVRAASRYNLALCQRQLGQPAEAKTTLLAYRTDHPGDKRAADVAYQLGDLHEA
                     AGENVEALEEFKKALDSNPSGALSVELLYRVGHSLEQQNDVDGALKSYNRAVASPDRK
                     NPFRLSALARCAALYEAKKDYARAYTAYRDIAQNSKDGELAAAAAGRAQQLLTLPKAT
                     KKR"
     gene            886..1542
                     /locus_tag="VFR25_07850"
     CDS             886..1542
                     /locus_tag="VFR25_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9941001.1"
                     /translation="MFENFDWIEAMRTSPVMCVILVCSVLTLGFAIERIIYFWKRRSN
                     PDALLASALVALRSGNKEEALKVCSVDRHPFGPVAAEVIKAMDEQPEAFEERLHIAMS
                     EQRLQLERNLGFIGTMGNVAPLIGLLGTVWGIMRAFHDMARTGSAGPSVVAAGVAEAL
                     FTTAAGLLVAVPAVLLYNNFVRRMGVMLTVTENHARTLRLSGGLRQSKPSVQTTVRVA
                     "
     gene            1553..1960
                     /locus_tag="VFR25_07855"
     CDS             1553..1960
                     /locus_tag="VFR25_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9941002.1"
                     /translation="MRRTRMRHEEAIHEPNMTPLIDVCLVLVIILLVSTPMAFQSSIG
                     VQRASKAGQKAAFTAKSERVEITLVSPDVVVVNQNQIPRASLQGLLKPLLEASTTKLV
                     IVRCGEGISHGAFVSVLDDAKACGAAQIAVVGG"
     gene            1962..2390
                     /locus_tag="VFR25_07860"
     CDS             1962..2390
                     /locus_tag="VFR25_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9941003.1"
                     /translation="MRRMRVPGYHLPITKVNVVPIIDVSLVLVIILLVTAPLMSVADL
                     PVDLPQARSRDSEDERNVSITLSSTGALAVDDEVVTADGLPSVLQTHLAKAGNNDVLV
                     VVRADSGAPYTAVQKLLEEARGAGATHLAIATRQKTEATR"
     gene            2387..3334
                     /locus_tag="VFR25_07865"
     CDS             2387..3334
                     /locus_tag="VFR25_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9941004.1"
                     /translation="MTAAALRIDQELVRRRTQWTMLISFVVHVLLFLWFTSFKTTTQD
                     LPIVTEVVLLEPGDLNPAAAAAPAPAPQRQEETVKGAPKEGQTDAHFIRMERSGDVAL
                     DPQVPNTFQDKLNSRLAAIQQQNSPLPVGTATGSPTASLFGSSPATVGAPGGTGTAPV
                     KLNRGGGTGGPPLELSRGGTPGAPASLAPASIQAGKSEAAAPSKGGGDTTARSTIAGA
                     SLAGPIADRPVLFYTLPTYPDWAKKEAVEGSVTLYFVVRPDGTVKENILVQKTAGFGD
                     FDDNARAALRAWRFEPLKNGRTGEQWGTITFHFRLRSAG"
     gene            3350..4645
                     /locus_tag="VFR25_07870"
     CDS             3350..4645
                     /locus_tag="VFR25_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941005.1"
                     /translation="MQAFPKFETKVSRVIAWLTALAALALFAAPRAKAEDATSSRPPV
                     SEAALQASQSTTTTTAPTPAPAKASTKSSKKSSKRSKSATKPSAEEAAPLTMTKEVSL
                     TESATTDSTGMTLRGGQERTDFKTMTIEGEDRVHVDVERPELQIDLDASKAHGLESGT
                     AADVMNRVAPDLTTKYLALSATQPTPYLGRPWLRQFATGPVAKFQPNVKGVERWKLVV
                     ADSKGELVRTFEGKGDPPKEITWDGRTQKGALVTPGLTYSYYMEAFDKAGNKRNFVGE
                     GFKVSAYRADTPAGPVLVFSGMSVLAGQKSSYGMASANRAVAPVILETVSWLNQSTRV
                     QQPVTIAVSARSYEQANLIAKQVGMTMTDLALGDPSRIKAVAEVAPDAADGGVVRVSL
                     AGADPTTGTAVNLSAPMDGKSKDGKKESKKDSKKSQNKK"
     gene            4681..5394
                     /locus_tag="VFR25_07875"
     CDS             4681..5394
                     /locus_tag="VFR25_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9941006.1"
                     /translation="MEGAPSSSGGEPEGPKADAERQSTFITTRFERWALPRLAASLPR
                     WVVPDHLTGLGILAATVVAVAYILTTRNPHWLWLANAALVVHWFGDSLDGTLARVRKI
                     ERPRYGFYLDHLTDAYSTTAIGLGLGFSPYMLLSVGLAIVIGYLILSINVYLETHVYG
                     KFRYGYGILGPTEARVILMGLNLIALLIGPIPFHVKGIELTVFDVLGIAAALSMAGLL
                     ARRVFRNLADLARAEPPRG"
     gene            5476..7071
                     /locus_tag="VFR25_07880"
     CDS             5476..7071
                     /locus_tag="VFR25_07880"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9941007.1"
                     /translation="MPPDTERIPKRLLHALGRAAARESQRRDIERQTRPDQLEKSVRE
                     YLGRTKIVIASNREPYQHVRRNGSVQTIRSAGGLASALDSVARATGAVWVAQASGDAD
                     REMADARGRVSMPPGQDRYTLQRVWIDDEERAEGFNRFVNGCLWPLCHIVYVRPQFVP
                     AHWRAYQEVNRMFAEAIVDAAGDEPALVLLQDYHLALCAAEIRARRPDLTIVLFWHIP
                     WPNAEVFRIVPWKREILEGLLACDLLGFHIRHHGMNFMETVSQELEALIDQERGAVRR
                     RGRRTFVRDYPISPDVAEIAVVAADPSTQAVCRRIREQLGIGDAQVILGVDRLDYTKG
                     IPERLNAYELLLDRHPEWRDRVVMIQIAVPSRVDLPEYQALGRNVSERVDQLNQRFGS
                     ERRKSVYLITENLDFRDLIPYYALADVMAVTSLHDGMNLVAKEYVAARVHNDGVLVLS
                     PYTGASRELEHAIQVSPYDIDRLSGAFRQALTISPEDRERRMKSLREVVASQNIYDWA
                     RKLLRDVRRLHIVPGTSWSGGRH"
     gene            7068..7880
                     /gene="otsB"
                     /locus_tag="VFR25_07885"
     CDS             7068..7880
                     /gene="otsB"
                     /locus_tag="VFR25_07885"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET9941008.1"
                     /translation="MTRPLLARGVWDRVARAIADNGSRPGHGGRRPLIAFDLDGTLAP
                     IVPHPSRARIPASTKRLLLALAADRGLVVAVVSARRARDLRRLLPERGIRRIGQYGLE
                     GAFAPSATERARWRRSAREIRRVLEPIAEATPGSWVEDKGMTVSLHDRGVAPSNLPAL
                     RRALRRAAVKAEQFGFSTEHGIRVTDFVPRGYDKGHAIEALRRGERGAPVIYFGDSDA
                     DEPAFAALGPDDVPVRVGPGRTRARFRARGTADVARFLRRLVELRRASATRR"
     gene            7884..>8477
                     /locus_tag="VFR25_07890"
     CDS             7884..>8477
                     /locus_tag="VFR25_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941009.1"
                     /translation="MDWHQIIPPDLGYQLQQTLLAVTGGLTLILIAGAIALIGWIVGA
                     LLSRAAQGLLSLAGIDGPAKRLAAEGGIRVDFLPSRMVGFSIFWLVILGTAIIALRVI
                     GLDLAPSLVARLQDVLPRIVTSGLVLLLGIPLALAAGRLLNALLIQSGIRPNRIREQS
                     TGALLVAFTVLIALDQLGLAAHLVLAVVIAVVAAAGLA"
BASE COUNT         1365 a         2968 c         2837 g         1307 t
ORIGIN      
        1 gacgcgctct tccagatcgc gaagtcgtcc tatcaggaca agaagtacgc cgaggccgcg
       61 gacggcttcc gccgcgtcgt gagccagttc cccggctatt cggccgcgga cgacgcgcag
      121 ttcctgctcg ccgactccta cgccaaggcg gggtccgccg agcagtcgcg cctcgcgtac
      181 gagcagttcc tcgccttctt cccgtcgagc gagcttcgcc cgacggcgga gttccagctc
      241 gggctccagt acttccaggg gcaggacttc atgcacgcgg cggtctcgtt cacgcaggtc
      301 ctgggcgaga gcgcgaccac ggacgtgcgc gcagcgtcgc gctacaacct cgcgctctgc
      361 cagcggcagc tcggccagcc ggcggaagcg aagacgacgc tcctcgcgta ccgcacggac
      421 caccccggcg acaagcgcgc cgcggacgtg gcgtaccagc tcggcgatct ccacgaggcg
      481 gccggcgaga acgtggaagc gctcgaggaa ttcaagaagg cgctcgactc gaatccttcg
      541 ggagcgctca gcgtggagct cctgtaccgg gtcggccaca gcttggagca gcagaacgac
      601 gtcgacggcg cgctcaagtc ctacaacagg gccgtggcgt cgcccgaccg gaagaacccg
      661 ttccgcctgt ccgcgctcgc ccgctgcgcg gctctctacg aggcgaagaa ggattacgcg
      721 cgggcctata cggcatatcg cgacatcgcg cagaactcca aggacggcga attggccgca
      781 gccgcggccg gccgcgccca gcagcttctg actctgccca aggcgacgaa gaagcgctaa
      841 cgaacggccg ccgcaaacgg ccaggtaccg aaaggagcga ggacgatgtt cgagaatttc
      901 gactggatcg aagcgatgcg cacgagcccc gtcatgtgcg tcatcctggt ctgcagcgtg
      961 ctcacgctgg gcttcgccat cgagcgcatc atctacttct ggaagcgccg gagcaatccg
     1021 gacgcgctcc tcgcgtcggc tctcgtggcc ctgcgctcgg gcaacaagga agaggccctc
     1081 aaggtctgct ccgtcgaccg ccatccgttc ggccccgtcg cggctgaagt catcaaggcg
     1141 atggacgagc agccggaagc gttcgaggag aggctccaca tcgcgatgag cgagcagcgg
     1201 ctccagctcg agcggaacct gggcttcatc ggcaccatgg gcaacgtggc gccgctcatc
     1261 ggcctcctcg gcaccgtgtg gggcatcatg cgcgcgttcc acgacatggc gcgcacgggc
     1321 tcggccggtc cgtcggtggt cgccgcgggc gtcgccgaag cgctcttcac cacggccgcg
     1381 ggtcttctcg tggcggtgcc ggctgtcctt ctctacaaca acttcgtgcg ccggatgggc
     1441 gtcatgctca cggtgaccga gaaccacgcg cggacgctgc gactgagcgg cggcctgcgc
     1501 cagtccaagc cttccgtcca gaccaccgtc cgcgtcgcgt aaggaggctc cggtgcgccg
     1561 aacccgcatg cgtcatgagg aggcgatcca cgagccgaac atgacgcctc tgatcgatgt
     1621 ctgcctggtg ctggtcatca tcctgctcgt ctcgacgccc atggcgttcc agtcgagcat
     1681 cggggtccag cgcgcttcca aggcaggaca gaaggccgcc tttacggcga agtcggagcg
     1741 ggtggagatc accctcgttt ctccggatgt ggtggtcgtg aaccagaacc agattccccg
     1801 ggcgagcctt caggggctct tgaagccgct cctcgaggcg agcaccacca agctcgtcat
     1861 cgtgcgctgc ggcgagggga tctcccatgg agcgttcgtg agcgtcctcg acgacgccaa
     1921 ggcctgcggc gcggcacaga tcgccgttgt gggaggctga catgcgtcgc atgcgcgtac
     1981 ccggatatca cctgccgatc accaaggtga acgttgttcc gatcatcgac gtctcgctgg
     2041 tgctcgtgat catcctcctc gtgacggcgc cgctcatgtc ggtggccgac cttccggtcg
     2101 acctgccgca ggcgcggtcg cgtgactccg aggacgaacg gaacgtgagc atcacgctgt
     2161 cgagcacggg agcgctcgcc gtggacgacg aggtcgtcac ggccgacggc cttccgtccg
     2221 tgcttcagac ccacctcgcc aaggctggga acaacgatgt gctcgtggtg gtgcgcgcgg
     2281 actcgggcgc gccctacacc gcggtccaga agcttctcga ggaggcgcgc ggggccgggg
     2341 ccacacacct cgcgatcgcg acacgccaga agacggaggc cacccggtga ccgccgccgc
     2401 acttcgcatc gatcaggagc tggtccgccg caggacgcag tggaccatgc tgatcagctt
     2461 cgtcgtccac gtgctccttt tcttgtggtt cacctcgttc aagacgacga cccaggatct
     2521 tccgatcgtg accgaggtcg tgctgctcga gccgggagat ctcaatcccg cggccgcggc
     2581 ggccccggcg ccggcgcccc agcgccagga agaaacagtg aagggagccc ccaaggaagg
     2641 ccagacggac gcgcatttca tccgcatgga gcggagcggc gacgtcgcgc tcgatcctca
     2701 ggtgcccaac accttccagg acaagctgaa ctcgcggctc gccgcgatcc agcagcagaa
     2761 ctcgccgctt ccggtcggaa cggccaccgg gtcccccacg gcctcgctct tcggatcgag
     2821 cccggcgacg gtgggcgcgc ccggcggcac cggcacagcg ccggtgaagc tcaaccgcgg
     2881 cggcggaacg ggcggtcctc cgctcgagct ctcgcgcggc ggcacgcccg gcgccccggc
     2941 ttcgctagcg ccggcttcca tccaggcggg caaatcggag gcggccgcgc cgtccaaggg
     3001 cggtggcgac acgacagcgc ggagcaccat cgcgggcgcc tcgctcgcgg gaccgatcgc
     3061 ggaccggccg gttctcttct acacgctgcc gacgtatccg gactgggcga agaaggaggc
     3121 cgtcgaagga tcggtgacgc tctacttcgt cgtgcggccg gacgggaccg tgaaggagaa
     3181 catcctcgtc cagaagacgg cgggattcgg tgatttcgac gacaacgccc gcgcggcgct
     3241 tcgcgcgtgg cgtttcgagc ccctgaagaa cggacggacc ggagagcagt ggggcacgat
     3301 cacgttccac ttccggctcc gcagcgccgg ttaaggagga gggagaccga tgcaggcctt
     3361 tccgaagttc gagaccaagg tctcgcgcgt catcgcgtgg ctgaccgcac tcgccgcgct
     3421 cgccctcttc gccgcgccgc gcgcgaaggc cgaggacgcg acgtcgtcgc gcccgccggt
     3481 ctccgaggcg gccctccagg cctctcagtc caccacgacg acgacggctc cgacgcctgc
     3541 gccggcgaag gcctcgacga agtcctccaa gaagtcgtcg aagcggtcga agagcgcgac
     3601 caagccttcg gcggaagagg cggcgcccct gacgatgacg aaggaagtga gcctcacgga
     3661 gtccgcgacc acggatagca ccggcatgac gcttcgcggc ggccaggagc gcaccgactt
     3721 caagaccatg accatcgagg gcgaggaccg cgtgcacgtg gacgtggagc gccccgagct
     3781 tcaaatcgat ctggatgctt ccaaggcgca cgggctggaa tccggcaccg cggccgacgt
     3841 catgaaccgg gtcgctccgg atctcacgac gaagtacctc gccctctcgg cgacccagcc
     3901 cacgccgtat ctcgggcgtc cgtggctccg tcagttcgcc acgggaccgg tggcgaagtt
     3961 ccagccgaac gtgaagggtg tcgagcggtg gaagctcgtg gtggccgact cgaagggtga
     4021 gctcgtgcgc acgttcgagg gcaagggcga tccgcccaag gaaatcacct gggacggccg
     4081 cacgcagaag ggcgccctcg tcactccggg gctgacctac tcctactaca tggaggcctt
     4141 cgacaaggcc ggcaacaagc ggaacttcgt gggcgagggc ttcaaggttt ccgcctaccg
     4201 tgcggacacg ccggcgggcc cggtgctcgt cttctccgga atgagcgtcc tcgcgggaca
     4261 gaagtcgtcg tacggcatgg cctcggcgaa ccgcgccgtg gcgccggtga tcctcgagac
     4321 ggtgagctgg ctcaaccagt cgacgcgcgt gcagcagccg gtcacgatcg cggtgtcggc
     4381 gcggagctac gagcaggcga atctcatcgc gaagcaggtc ggcatgacga tgaccgacct
     4441 cgcgctcggc gatccctcgc ggatcaaggc cgtggctgag gttgctccgg acgcggcgga
     4501 cggcggcgtc gtgcgtgtgt cgctcgcggg cgcggatccg acgacgggca ccgcggtgaa
     4561 tctctccgcc ccgatggacg gcaagagcaa ggacgggaag aaggagtcca agaaggactc
     4621 gaagaagagc cagaacaaga agtagacccg cgcccctgat cgcgggcatg atggcgcgcc
     4681 atggagggcg cgccttcgtc gagcggcggt gagccagagg gtccgaaagc ggacgccgaa
     4741 cgccaatcga ccttcatcac gacccgattc gagcgctggg cgcttccgcg cctcgccgcc
     4801 tcgcttcccc gctgggtcgt ccccgaccat ctgaccggac tcgggatcct cgccgccacc
     4861 gtggtcgcgg tcgcgtacat cctcaccacg cggaacccgc actggctctg gctcgcgaac
     4921 gcggcgctcg tggtccactg gttcggggac agcctcgacg gaacgctcgc ccgcgtccgg
     4981 aagatcgagc gccctcgata cggcttctac ctcgaccatc tgaccgacgc gtactccacg
     5041 acggcgatcg gcctggggct aggcttctcg ccctacatgc tcctctcggt gggtctcgcc
     5101 atcgtgatcg gctatctcat cctgtcgatc aacgtgtacc tggagaccca cgtgtacggg
     5161 aagttccgct acgggtacgg catcctcggc cccacggagg cgcgggtcat cctcatggga
     5221 ctgaacctga tcgctctcct gatcgggccc attcccttcc acgtgaaggg catcgagctc
     5281 accgtgttcg acgtgctcgg gatcgccgcc gcgctgtcca tggcggggct cctggcgcgc
     5341 cgcgtgttcc ggaacctcgc ggatctcgcc cgggcggagc cgccccgagg ctgacgtcgt
     5401 aacccctgtt cgacacatcg cctagccgga cctcttccca ggtctagccc ctatctgttc
     5461 tagaatggaa ggacgatgcc accggacacc gagcgcattc cgaaacgcct cctccatgcc
     5521 ctgggccggg ccgccgcgcg cgagagccag cggcgggaca tcgagcgcca gacccgcccc
     5581 gaccagctcg agaagagcgt ccgcgagtac ctggggcgca ccaagatcgt catcgcgagc
     5641 aaccgcgagc cgtaccagca cgtgcggcgg aacggctcgg tccagacgat tcgaagcgcc
     5701 ggcgggctcg cgtccgcgct cgattcggtc gcccgggcga ccggggcggt ctgggtggcc
     5761 caggcatccg gcgacgccga ccgcgagatg gcggacgcgc gcgggcgcgt gtccatgccc
     5821 cccggtcagg accggtacac cctccagcgg gtctggatcg atgacgagga gcgggccgag
     5881 gggttcaacc ggttcgtgaa cggctgcctc tggccgctct gccacatcgt ctacgtgcgt
     5941 ccccagttcg tgcccgcgca ctggcgcgcg taccaggaag tgaatcgcat gttcgccgag
     6001 gccatcgtcg acgccgcggg agacgagccg gcgctcgttc ttttacagga ttatcacctc
     6061 gcactctgtg cggcggaaat tcgcgcgcgg cgccccgatc tcaccatcgt cctcttctgg
     6121 cacatcccct ggccgaacgc cgaagtgttc cggatcgtgc cgtggaagcg cgagatcctc
     6181 gaaggactcc tcgcgtgcga tctcctcggc ttccacatcc gccaccacgg gatgaacttc
     6241 atggagacgg tgtcgcagga gctcgaggcg ctgatcgacc aggagcgggg cgccgtgcgc
     6301 cgccgggggc ggcggacgtt cgtccgcgac tatccgatca gtccggacgt ggccgagatc
     6361 gccgtggtcg cggccgatcc ctcgacccag gcggtctgcc gccggatccg cgagcagctc
     6421 gggatcgggg acgcgcaggt gatcctgggc gtcgaccggc tcgactacac gaaagggatc
     6481 ccggagcggc tcaacgccta tgagctcctc ctcgatcgcc atcccgagtg gcgcgaccgc
     6541 gtggtgatga tccagatcgc ggtcccgagc cgcgtcgatc tccccgagta tcaagcgctc
     6601 ggccgcaacg tgtccgagcg cgtggaccag ctcaatcagc gcttcgggtc ggagcgccgg
     6661 aagtcggtct atctcatcac cgagaacctc gacttccgcg atctcatccc ctactacgcc
     6721 ctcgccgacg tgatggcggt cacgtcgctc cacgacggga tgaacctcgt ggcgaaggag
     6781 tacgtcgcgg cgcgcgtgca caacgacggc gtgctggtcc tctcccccta cacgggcgcc
     6841 tcgcgcgagc tggagcacgc gatccaggtg agcccgtacg acatcgaccg cctgagcggc
     6901 gccttccgcc aggcgctcac catcagcccc gaggatcgcg agcggcgcat gaagtcgctc
     6961 cgcgaggtcg tggcgtccca gaacatctac gactgggcaa gaaagctcct gcgcgacgtg
     7021 cgccgcctgc acatcgtgcc gggaacgagc tggtccggcg ggcggcattg acgcgcccgc
     7081 ttctggcgcg cggcgtctgg gatcgagtgg cacgcgcgat cgcggacaac gggtcccggc
     7141 ccggccacgg cgggcgaagg ccgctcatcg ccttcgacct cgatggcacc ctcgcgccca
     7201 tcgttcccca tccgtcgcga gcccggatcc cggcctcgac gaaacggctc ctcctggcgc
     7261 tcgcggccga tcgcgggctc gtggtggcgg tcgtgagcgc gcggcgcgcg cgggacctgc
     7321 gtcggctcct gcccgagcga ggcatccggc ggatcggtca gtacgggctc gaaggcgcgt
     7381 tcgcgccgtc ggcgacagag cgcgcacgct ggcgccggag cgcccgggag atccggcgtg
     7441 tgctcgagcc gatcgcggag gcgaccccgg gttcgtgggt cgaggacaag gggatgacgg
     7501 tttcgctgca cgatcggggc gtggcgccct cgaaccttcc cgcgctccgg cgagcactcc
     7561 ggcgtgcggc ggtcaaggca gaacaattcg ggttttccac ggagcacggc atccgggtga
     7621 ccgacttcgt gccgcgaggg tacgataaag gacatgcgat cgaggcgctc cgacggggcg
     7681 aacggggggc tcccgtgatc tacttcggag acagcgacgc cgacgagccc gcctttgccg
     7741 cgctcggccc ggacgatgta ccagtccgcg tcggccccgg cagaacgcgc gcacgcttcc
     7801 gcgcgcgcgg cacggccgac gtggcgcgat tcctccgcag gctcgtcgag ctgcggaggg
     7861 catccgcaac caggaggtaa tccatggatt ggcatcagat cattcctccc gatctcggat
     7921 atcagctcca gcagacgctc ctcgctgtga ccggagggct cacgctcatc ctgatcgctg
     7981 gggccatcgc gctcatcggc tggatcgtcg gcgcgctcct ctcgcgcgcg gcccagggtc
     8041 tcctctccct cgccgggatc gacgggcccg cgaagcgcct cgccgccgag ggcgggatcc
     8101 gcgtggactt cttgccgagc cgcatggtcg gcttctccat cttctggctg gtgatcctgg
     8161 gcaccgcgat catcgccctc cgcgtgatcg gcctcgacct cgcgccgtcg ctcgtggcgc
     8221 ggctccagga cgttctcccg cgcatcgtga cctcggggct cgtgctcctt ctcggcattc
     8281 ccctcgcgct cgccgcggga cggctcctga acgcgcttct cattcaatcg ggaatacgtc
     8341 ccaaccggat ccgcgagcaa agcaccggcg cgctcctcgt cgccttcacg gtgctgatcg
     8401 ccttggatca gctcggcctc gccgcgcacc ttgtgctggc ggtggtcatc gcggtcgtcg
     8461 ccgcggccgg actcgcg
//
LOCUS       DASTKC010000192         9437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_394481, whole genome shotgun sequence.
ACCESSION   DASTKC010000192 DASTKC010000000
VERSION     DASTKC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394481"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /gene="larB"
                     /locus_tag="VFR25_07895"
     CDS             <1..455
                     /gene="larB"
                     /locus_tag="VFR25_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET9941010.1"
                     /translation="SSKAKARRKRGTEPVLVISAGTSDVPVAEEAVATLEFMGVPVTR
                     VYDAGVAGIHRLVAHVKALRSAAAIVVVAGMEGALASVVAGLTDRPVIAVPTSVGYGA
                     SYGGLAALLAMLNSCVAGVTVVNIDNGFGAGYAAGVIARQSKNGRRPR"
     gene            452..1633
                     /gene="larC"
                     /locus_tag="VFR25_07900"
     CDS             452..1633
                     /gene="larC"
                     /locus_tag="VFR25_07900"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET9941011.1"
                     /translation="MTIVYFDCVAGASGDMILGSLVAAGAPLAEIDRNLRALPLEGFT
                     LAEERVERHGFEAFQLQVTTQETKSHRHFTEIKRLLEGAKLPKRVLERALGTFEILGR
                     AEAEAHKVPLEKVHFHEVGAIDAIVDIVGTAWALELLGVDRCHASVIPQGRGFVKAAH
                     GILPIPAPATLKILEGVPVRQTEIEAELTTPTGAALLKALCASIGDPVGIRPSRVGVA
                     TGTMDLKERPNVLRAIVGEPLAGDGAGSVEVLETTIDDMNPQLYGHLTEALFESGAAE
                     VFLTPVQMKKGRPGVLVTALADPRRSEAVAARLFAESSTIGIRVRREGRIELRRSISE
                     VETPLGKVHVKTVVLPSGEERRVPEYEDLRRIARATGRPLIEVMEEIRGHLIEGARSA
                     S"
     gene            1630..3312
                     /locus_tag="VFR25_07905"
     CDS             1630..3312
                     /locus_tag="VFR25_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9941012.1"
                     /translation="MIRLEGASASVPGPSRAESPRILLDPIDLDLRAGESHLVLGANG
                     SGKSTLVRMLAGLRPPASGRVLLDGAPLAPGRVWPSVAVLFEEPDPQFLADTVEGEIA
                     FGLESLALPPDEIRSRTAAAMERYQVAELARREPYSLSGGEKARVLLAAMMAAAPRAI
                     LLDQALAHLDPETRRALERDLVERALAGECLLVRTHQDWDPPLPGETLHVIERASLRK
                     VDRDSVAPRSADVPYPLALRAAALLRECGRWTGPLAMTVAELRAGLGASDHAPRASIY
                     NTPSVTGDPAISLSGASWSPPGERRTLAPIDLEVRAGEVVALVGRSGSGKSTILKLAA
                     GLMPPSTGSVRAARGAGLALEYPERQLFGRTVEEDVAALLWVAGVPERERHDRARVAL
                     ETVGLDFDRFGPRVPATLSEGEKRRAAIAALLADRPATLLLDEPTAGLDPGGRASLAR
                     AIAAVKEEGHAVLFASHDLDFVSGVADRVAVLARGDDGSTALATGPGAAIWRDAALLD
                     RAGLPAPEFVALADTFRGAGLLRDDDVRDGSSLLGALARSLDATHGAVGSSA"
     gene            3312..5171
                     /locus_tag="VFR25_07910"
     CDS             3312..5171
                     /locus_tag="VFR25_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941013.1"
                     /translation="MRATPPRPRRRARVHAPGRSGVSTSAVWGLVAIAFLALVLRLAF
                     VAGASRHPVETHLELDPAVHDANAWAIASGQEPEPGPYFHAPLYTRALALVYSVAGHN
                     PAAARTAQAVLGALTVLLIGWITARLWGPRTGWIAAALAAVYPPLIFFNGQLVSATLE
                     VALTALSLILTLRAGDRPRSPWIAAAAGFALSLSAITRPTVLPFELIAAAWLAARGVG
                     RKSVALYLAAALSLPVAVTARNAIVGKDAVFIASQGGINFYIGNHAGADGTTADVPGA
                     GSGITATQEVPARLASAAVGRPLKPSEVSSYWFQRGMEFWVKKPFAAMRLYVRKVLLM
                     WNRRELPNLVDQQFFLPMYSWLYRIPVWPGFAILAPIALAVAWRERGRAALLLLYLAT
                     STAVTAAFFVCDRFRLPLVLGVIPLAAAGIDSLVSAAEEARSRGASAPVHLGTVAMFL
                     AALALVWAPFPRLQRTQAGMSWYRVARAEAASGDLTAARNSYRNAEQAGLNRTEFWNE
                     YGVFRMQNGDPMGAESLFRRALMLNSHFGPAHANLAELYYRRETYEAAAGEYAIAATL
                     IPERAAELWVNAGTVYEGLGQKDRALDCYQKALAAKPGFESAMEGERRVGGAP"
     gene            complement(5190..5612)
                     /locus_tag="VFR25_07915"
     CDS             complement(5190..5612)
                     /locus_tag="VFR25_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941014.1"
                     /translation="MKKGAVWALVVVLAALAVWFALAWRGAERRAEKAQRVSVQPVYN
                     RVLLDEIELAELKERGLEDPIPALKADLSSRKDLIKFDSGVGGTMAFYDKDGMVFLPG
                     GYLYAPAEDGHYLMHVLLSYEVAPGGKITWKVVSAKRD"
     gene            5793..6362
                     /gene="pyrR"
                     /locus_tag="VFR25_07920"
     CDS             5793..6362
                     /gene="pyrR"
                     /locus_tag="VFR25_07920"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9941015.1"
                     /translation="MPTGESVEAVREKAEILDGEGLRRAITRIAHEIIEKNGGAEGLV
                     LAGIRRRGYPLAQRIAAKIREFEGVDLPVGALDITLYRDDLQTIDHQPVVGPTDIPVD
                     IDGKVVVLVDDVLYTGRTVRAALDELIDLGRPRAIRLAVIVDRGHREIPIRADFVGKN
                     VPTSKREVVMVKLEEIDRHDGVSIGEILT"
     gene            6359..7360
                     /locus_tag="VFR25_07925"
     CDS             6359..7360
                     /locus_tag="VFR25_07925"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9941016.1"
                     /translation="MIGGKKDLLGLEDLSAQEITGILDTARTFREVLDRPIPKVPPLR
                     GMTAANLFFEASTRTRLSFELAEKRLSADTVNFQTSGSSVSKGETLRDTARNIEAMGI
                     HLVVVRHQASGAPHYLARHLDAGVINAGDGTHEHPTQGLLDLYTMRAERGRIAGLKVA
                     IIGDVMHSRVARSNIWGLTKLGAEVTIAGPPTMMPAEVERFGVRVAKSVDEAIEGSDV
                     VNILRIQLERQRGALYPSLREYARVYGVTRERLRRARPDVTVMHPGPMNRGVEIAQDV
                     ADGEHSVILQQVTNGVAVRMAVLYLLSGRSEPPEAPSETPTVEATKSHDEPTLSPRR"
     gene            7329..8648
                     /locus_tag="VFR25_07930"
     CDS             7329..8648
                     /locus_tag="VFR25_07930"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9941017.1"
                     /translation="MTSPLCLRGGRILDPARGIDRAQDLWIDQGKVAGLGEDAPAALR
                     SRKDVQIVDVRGAVVCPGLVDIHVHLREPGQEEKETIETGVRAAVRGGFAAVACMPNT
                     SPPLDDRPRIEYVRRRAAELGLARVFPIAAVTVGQQGEQLVEIEDLVDAGAVAITDDG
                     KPVRNAEIMRRALELTRELGIPVIQHAEDPDLKGTGVMHEGWVSTRIGLKGIPDIAES
                     VIVARDALLAERTGGRVHVAHVSAARSVQIIRQAKARGIHMTGETAPHYLLLTDEAVA
                     GYSPSAKMNPPLRSERDRDALIEAIVDGTLDCLATDHAPHTDIEKDADFDSAPFGIVG
                     LETALGCYLKALVEPGHLSLMDLIERMTVGAYRVLGRPGGTLEAGAEADVTVFDPESR
                     FTVRASEFESKGRNTPFEGWDLPGRVLMTVVGGRIAYQTATSEATLR"
     gene            8684..>9437
                     /locus_tag="VFR25_07935"
     CDS             8684..>9437
                     /locus_tag="VFR25_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941018.1"
                     /translation="MLKRFGGSSRPWTLGLLLLLAGGGCAYYNTFYLAKKNYREGNKA
                     QERSLSEAPAQEAIGRYDVVIRQCQKVLTDYPKSKYVDDAAYMMGASYYGKGDYPNAI
                     QQLTSFASKYPKSPYRADARFTEGLARYRRKEFVESDSIFREVEGQYPDFSRRWELYF
                     YSGETQTQLKHYTSAAHWYRLADKRASGRREKSKTLRRLGDAYLSAEHADSAEVAYAE
                     CLKVEERGSQRVDAAIARGDALRELGRYPEALQ"
BASE COUNT         1404 a         3366 c         3257 g         1410 t
ORIGIN      
        1 cgagctccaa ggcgaaggca cgcaggaagc gcggtaccga accggtgctc gtgatcagcg
       61 ccgggacctc cgacgttccg gtcgccgagg aggccgtggc gacgctcgag ttcatgggcg
      121 tgccggtgac gcgggtgtac gatgctgggg tcgccgggat ccatcgcctg gtcgcccacg
      181 tgaaggcgct ccggagcgcc gcggcgatcg tcgtggtcgc gggcatggaa ggggcgctcg
      241 cgagcgtcgt ggcggggctc accgaccgtc cggtgatcgc ggtgccgacg agcgtcggct
      301 acggcgcctc ctacggaggg ctggccgcgc tcctcgcgat gctcaactcc tgcgtggccg
      361 gggtcacggt cgtgaacatc gacaacggat tcggcgccgg ctacgccgcc ggcgtgatcg
      421 ccaggcagtc caagaacggg agacgtccac gttgaccatc gtctacttcg actgcgtcgc
      481 cggcgcgtcc ggggacatga tcctgggatc gctcgtcgcg gcgggcgcgc cgctcgcgga
      541 aatcgatcgc aatctccgcg cgctcccgct cgagggcttc accctcgcgg aggagcgcgt
      601 cgagcggcac ggcttcgagg cgttccagct ccaggtcacg acgcaggaaa ccaagtctca
      661 ccgccacttc actgagatca aacgcctgct cgaaggcgcg aagcttccca agcgagtgct
      721 cgagcgcgcg ctcgggacgt tcgagatcct ggggcgcgcg gaggccgagg cacacaaggt
      781 gccgctggag aaggttcact tccacgaggt gggcgcgatc gacgcgatcg tcgacatcgt
      841 gggcactgca tgggcgctcg agctcctcgg cgtggatcgc tgccacgcga gcgtgatccc
      901 gcagggacgc ggcttcgtca aggcggccca cggcatcctt cccatccccg cgcccgcgac
      961 gctcaagatt ctcgagggcg tcccggtgcg ccagaccgag atcgaggccg agctgacgac
     1021 gccgaccggc gccgcgctct tgaaggcgct ctgcgcgtcg atcggcgatc cggtcgggat
     1081 tcgcccgtcg cgcgtcggcg tggcgacggg gacgatggac ctgaaggagc gccccaacgt
     1141 gctccgcgcg atcgtgggcg agccgctcgc cggcgacggc gcggggtccg tcgaggtgct
     1201 cgagaccacg atcgacgaca tgaacccgca gctctacggg cacctcaccg aggccctctt
     1261 cgaatcgggc gcggccgagg tcttcctcac gccggtgcag atgaagaagg gccgcccggg
     1321 cgtcctcgtg acggcgctcg cggatccgcg ccgctccgaa gccgtggccg cccgtctctt
     1381 cgccgaatcc tcgacgatcg gcatccgcgt ccgccgcgag ggacggatcg agctcagacg
     1441 ctcgatctcg gaagtggaga ctccgctcgg caaggtgcac gtgaagacgg tggtgctccc
     1501 ctcgggcgag gagcgccgcg ttcccgaata cgaagatctc cgccgcatcg cgcgcgccac
     1561 gggccgtcct ctgatcgagg tcatggagga gatccgcggc catctgatcg agggcgcccg
     1621 ctcggcctca tgatccggct cgagggcgca tccgcgtccg tgccgggccc gtcccgcgcc
     1681 gagagccctc gcattctcct cgatccgatc gatctcgacc ttcgcgcggg cgagagccac
     1741 ctcgtcctgg gcgcgaacgg atccggcaag tccacgctcg tccggatgct cgccggcctc
     1801 cgcccccccg cgtcgggacg cgttctcctc gacggcgctc cactggcgcc ggggcgcgtg
     1861 tggccttccg tggccgtcct cttcgaagag cccgatcccc aattcctcgc cgacaccgtg
     1921 gagggagaga tcgcgttcgg gctcgagtct ctcgcgcttc caccggatga gatccggtcc
     1981 cgaacggccg ccgcgatgga gcgataccag gtggccgagc tcgcgcggcg cgagccgtat
     2041 tccttgagcg gcggggagaa ggcgcgcgtg ctcctcgccg cgatgatggc ggccgcgccg
     2101 cgcgcgatcc ttctcgacca ggcgctcgcg catctggatc ccgagacccg gcgcgcgctc
     2161 gagcgggacc tcgtggagcg ggcgctggcg ggggagtgcc ttctcgtccg cacgcatcag
     2221 gattgggatc cgccgcttcc cggcgaaacc ctgcacgtca tcgagcgcgc gtcgctccgg
     2281 aaggtcgatc gtgacagcgt ggcgccccgg agcgcggacg ttccatatcc gctcgcgctc
     2341 cgggcggccg cgcttctgag ggagtgcgga cgctggaccg gtcccttggc catgacggtc
     2401 gcggaacttc gcgccggcct cggcgcttcg gaccatgcgc ctcgagcgtc catctacaac
     2461 acaccgtccg tcacgggtga tcccgcgatc tctctgagcg gagcctcgtg gtccccgccg
     2521 ggcgagcgcc ggacgctcgc gccgatcgat ctcgaggtgc gcgcgggcga ggtggtggcg
     2581 ctcgtcggac ggagcggctc ggggaagagc acaatcttga agttagcggc gggactcatg
     2641 ccgccctcga ccggctccgt ccgcgcggcg cgcggcgccg ggctcgcgct cgagtatccg
     2701 gagcgccagc tcttcggtcg cacggtggag gaggacgtgg ccgcgctcct ctgggtggcc
     2761 ggagttcccg aacgcgagcg ccacgatcgg gcccgagtcg cgctcgagac cgtgggcctc
     2821 gacttcgatc gcttcgggcc gcgtgtccct gcgaccttga gcgagggcga gaagcgccgg
     2881 gccgcgatcg ctgcgctcct cgcagaccgc cccgcgacgc tcctcctcga cgagccgacc
     2941 gcgggactcg atcccggcgg ccgagcctcg ctcgcgcgcg ccatcgcggc ggtgaaggag
     3001 gaggggcatg ccgtgctctt cgcgagccac gatctcgatt tcgtctcggg cgttgccgat
     3061 cgcgtggccg tgctcgcgcg cggggacgac gggtcgaccg cgctcgcgac cgggccagga
     3121 gcggcgatct ggcgcgacgc ggcgctcctc gaccgggcgg gacttcccgc accggagttc
     3181 gtcgctctgg ccgatacgtt tcgcggcgca gggctgctcc gggacgatga cgtccgcgac
     3241 gggtcgtcgc tcctcggagc gctcgcacgc tcgctcgacg cgacgcacgg cgcggtgggg
     3301 tcgagcgcgt gatgcgcgcc acacctccac ggccgcgccg ccgcgcccgg gtccacgctc
     3361 caggacgctc gggagtctcg acgtcggccg tgtggggcct cgtggcgatc gcgttcctcg
     3421 cgctcgttct aaggctcgcg ttcgtcgccg gcgcgtcccg ccacccggtc gagacccatc
     3481 tcgagctcga ccccgcggtc cacgacgcca acgcgtgggc gatcgcgagc gggcaggaac
     3541 ccgagccagg gccctacttc cacgcgcctc tctacacccg cgctctcgcg ctcgtctatt
     3601 cggtcgcggg gcacaatccg gccgccgcac gcaccgcgca ggcggtgctc ggcgctctga
     3661 cggttcttct catcggatgg atcacggcgc gactgtgggg cccgcgcacg ggatggatcg
     3721 ccgccgcgct cgccgcggtg tatccgccgc tgatcttctt caacgggcag ctcgtctccg
     3781 ccacgctcga ggtggcgctc accgcgctgt cgctcatcct cacactccgg gccggagacc
     3841 ggccgcgctc tccatggatc gcggccgctg ccggcttcgc gctctctctg tccgcgatta
     3901 cgcggccgac ggtgcttccg ttcgagctga tcgccgcggc ctggctcgcc gcccgcggag
     3961 tcggacgcaa gtccgtggcg ctctacctgg ccgcggccct ctcgctcccg gtcgccgtga
     4021 ccgcgaggaa cgcgatcgtg gggaaggacg cggtcttcat cgcgtcgcag ggcgggatca
     4081 acttctacat cggcaatcac gcaggagcgg acggcacgac ggccgacgtt ccgggcgcgg
     4141 gctccgggat cacggcgacg caggaggtgc ccgcgcgcct cgcatcggcc gcggtaggcc
     4201 gacccctgaa gccgtccgaa gtctcgtcgt actggttcca gcgcgggatg gagttctggg
     4261 tgaagaagcc gttcgccgcg atgcggctct acgtgcgcaa ggtcctcttg atgtggaatc
     4321 gccgcgagct gccgaatctc gtcgaccagc agttcttcct gccgatgtac tcctggctct
     4381 atcgcatccc cgtctggccc ggcttcgcga tcctggcgcc gatcgcgctc gcggtggcat
     4441 ggcgcgagcg cggccgcgcc gcgcttctcc ttctctatct cgcgacgtcc acggccgtca
     4501 ccgcggcctt cttcgtgtgt gaccggttcc ggcttccgct cgtgctcggt gtgatcccgc
     4561 tcgccgcggc cggcatcgac agcctcgtga gcgcagccga ggaggcgagg tcacgtgggg
     4621 cgagcgcgcc cgtgcatctc ggcacggtcg cgatgttcct cgcggcactc gcgctcgtgt
     4681 gggcgccgtt ccccaggctt cagcgcacgc aggcggggat gagctggtat cgcgtggcgc
     4741 gcgcggaggc cgcctcgggc gacctcacgg cggcgcggaa ctcgtacagg aacgccgagc
     4801 aggcgggcct gaaccggacc gaattctgga acgagtacgg cgtcttccgg atgcagaacg
     4861 gcgatcccat gggggcggag tcgctcttcc gccgcgcgct catgctgaac tcacacttcg
     4921 gccccgcaca cgcgaacctg gcagagctct actaccggcg cgagacctac gaggcggcgg
     4981 ccggggaata cgcgatcgcg gcgaccttga ttcccgagcg cgccgcggag ctctgggtca
     5041 acgcgggaac ggtctacgaa gggctggggc aaaaggaccg ggcgctcgat tgctaccaga
     5101 aggcgctggc cgcaaagccg ggcttcgaat ccgcgatgga aggagagcga cgggtcgggg
     5161 gcgcgcccta gctccgcggc ctcagggcct caatcccgct tcgcgctcac gaccttccac
     5221 gtgatcttcc cgccgggcgc gacctcgtag ctcaggagca cgtgcatgag gtagtggccg
     5281 tcctccgcgg gggcgtagag gtatccaccc ggcaggaaga ccatcccgtc cttgtcgtag
     5341 aaggccatcg tgccgccgac cccgctgtcg aacttgatga ggtccttgcg gctcgagagg
     5401 tccgccttca gcgcggggat cgggtcctcg aggccccgct ccttgagctc ggccagctcg
     5461 atctcgtcca ggagcacccg gttgtagacc ggctgcaccg acacgcgctg cgccttctcc
     5521 gcccggcgct ccgcgcctcg ccaggccagg gcgaaccaga ccgccaaggc agcgagcacc
     5581 accaccaacg cccacaccgc acccttcttc atcgcaccct ccccagtccc cctcaaccat
     5641 tcctccccca gcgcccgcat tctatgctat tctcccctcg ttctagcggc cgcccggccg
     5701 cgcaccggca cccttcaaac tagttccgag agactagaaa ggggctcgcc tccgagcacc
     5761 cccttgccat agtcccggaa cgggatggac gcatgcccac cggcgaatcc gtggaagccg
     5821 tacgcgagaa ggccgagatc ctcgatggcg aagggcttcg ccgcgccatc acgcggatcg
     5881 cgcacgagat catcgagaag aacggcggcg cggagggcct cgtcctcgcc ggcatccgcc
     5941 gccgcggcta tcccctcgcg cagcgcatcg ctgccaagat ccgtgaattc gagggggtcg
     6001 acctgcccgt cggcgcgctc gacatcacgc tctatcggga cgacctccag acgatcgacc
     6061 accagcccgt cgtgggaccg accgacatcc cggtcgacat cgacggcaag gtggtggtgc
     6121 tcgtcgacga cgtcctctac acggggcgca cggtgcgcgc cgcgctcgac gagctgatcg
     6181 acctcggccg tccgcgcgcg atccgcctcg cggtcatcgt ggaccgcggg caccgcgaga
     6241 tcccgatccg cgccgacttc gtcggcaaga acgtgcccac ctcgaagcgc gaggtggtga
     6301 tggtgaagct cgaggagatc gaccgccacg acggggtctc gatcggggag attctcacat
     6361 gatcggcggc aagaaagacc tgctcgggct cgaggatctc tccgcgcagg agatcaccgg
     6421 gatcctcgac accgcgcgga ccttccgcga ggtgctggac cggccgattc cgaaggtgcc
     6481 gccactccgc ggcatgacgg cggcgaatct cttcttcgag gcgagcacgc gcacgcgcct
     6541 ctcgttcgag ctggccgaga agcgcctgag cgcggacacc gtgaacttcc agacctcagg
     6601 ctcgagcgtc tcgaagggcg agaccctccg cgacaccgcg cggaacatcg aggcgatggg
     6661 gatccatctc gtggtcgtgc ggcaccaggc ttcgggggcg ccgcactacc tcgcgcgcca
     6721 tctggacgcg ggcgtgatca acgccggcga cgggacgcac gagcacccga cgcagggact
     6781 cctcgacctc tacaccatgc gcgccgagcg cgggcgcatt gcggggctca aggtcgcgat
     6841 catcggcgac gtgatgcaca gccgcgtcgc gcgctccaac atctgggggc tcaccaagct
     6901 cggcgcggag gtgacgatcg cggggccgcc cacgatgatg cccgcggagg tggagcgctt
     6961 cggcgtccgg gtggcgaaga gcgtggacga ggcgatcgag ggatccgacg tggtgaacat
     7021 cctccggatc cagctcgagc gccagcgcgg cgccctctat ccctcgctcc gcgagtacgc
     7081 ccgggtctac ggcgtcacgc gcgagcggct ccgccgcgcg cgccccgacg tcaccgtgat
     7141 gcaccccggc cccatgaacc gcggcgtcga gatcgcgcag gacgtcgccg acggcgagca
     7201 ctcggtgatc ctccagcagg tcacgaacgg cgtcgccgtg cgcatggcgg tgctctatct
     7261 cctctcgggg cgcagcgagc ccccggaggc tccatccgaa acccctaccg tggaggcgac
     7321 caagagccat gacgagccca ctctgtctcc gcggcggtag gatcctcgat cccgcccgcg
     7381 gcatcgaccg cgcgcaggat ctctggatcg atcaggggaa ggtcgccggg ctgggcgagg
     7441 acgcccccgc ggcgctccgc tcgcgcaagg acgtccagat cgtggacgtg cgcggcgcgg
     7501 tcgtgtgccc cggactcgtc gacatccacg tccacctgcg cgagcccggg caggaggaga
     7561 aggaaaccat cgagaccggc gtccgcgcgg cggtgcgcgg cggcttcgcc gcggtcgcct
     7621 gcatgcccaa cacgtcgccc cccctcgacg accggccccg catcgagtac gtgcggcggc
     7681 gcgcggcgga gctcggcctc gctcgcgtct tcccgatcgc ggcggtgacg gtggggcagc
     7741 agggggagca gctcgtcgag atcgaggacc tggtcgacgc gggcgcggtc gcgatcacgg
     7801 acgacggaaa gccggttcgg aacgcggaga tcatgcgccg cgcgctcgag ctgacgcgcg
     7861 agctcgggat cccggtcatc cagcacgccg aggatccgga tctcaagggc acgggcgtca
     7921 tgcacgaagg atgggtctcg acccgcatcg ggctgaaggg catccccgac atcgcggaat
     7981 cggtgatcgt ggcccgcgac gcgctcctcg ccgagcggac cggcggacgg gtgcatgtcg
     8041 cgcacgtgag cgcggcccgc tccgtccaga tcatccgcca ggcgaaggcg cgcgggatcc
     8101 acatgaccgg cgagaccgcc ccgcactacc tcctcctgac ggacgaggcg gtggcgggct
     8161 actcgcccag cgcgaagatg aacccgccgc tccgctcgga gcgcgaccgg gacgccctga
     8221 tcgaggcgat cgtggacggc acgctcgact gcctcgcgac cgaccacgcg ccccacacgg
     8281 acatcgagaa ggacgccgac ttcgattcgg ctccgttcgg aatcgtcggg ctcgagacgg
     8341 ctctcggctg ctacctgaag gccctcgtcg agccgggcca tctctcgctc atggatctga
     8401 tcgagcggat gacggtgggg gcgtaccggg tgctgggccg gcccggcggg acgctggaag
     8461 cgggagcgga agccgacgtt acggtgttcg accccgagag tcgcttcacc gtccgtgcct
     8521 cggaattcga gtcgaagggg cgaaacaccc ccttcgaagg ctgggacctt cccggccgcg
     8581 tgctcatgac cgtggtaggc ggccgaattg cctaccagac agcgacttcc gaggcgaccc
     8641 ttcgatagcc aaccacgctc gcgcgtggta cattcgggtg ctcatgctca aacgattcgg
     8701 gggatcgtcc cgtccctgga ccctcggcct cttgctcctc ctcgccgggg gcggatgtgc
     8761 ctactacaac acgttctatc tcgcgaagaa gaactaccgc gaggggaaca aagcccagga
     8821 gcggagcctg tcggaggcgc cggctcagga ggcgatcggc cggtacgatg tggtcatccg
     8881 ccagtgccag aaggtgctca cggactaccc caagagcaag tacgtggatg acgccgcgta
     8941 catgatgggc gcctcgtatt acggcaaggg tgattacccg aacgccatcc agcagctcac
     9001 gtcgttcgcg tccaagtacc cgaagagccc gtaccgcgcc gacgcgcggt tcaccgaggg
     9061 gctcgcccgg taccggcgga aggagttcgt ggaatcggac tcgatcttcc gcgaggtcga
     9121 gggccagtat cccgacttct cgagacgctg ggagctctac ttctactcgg gtgagaccca
     9181 gactcagctc aagcactaca cgtcggcggc gcactggtac cggctggcgg acaagcgcgc
     9241 ctccggccgc cgcgagaagt ccaagacgct gcgccggctt ggggacgcgt atctctccgc
     9301 ggagcacgcg gactcggccg aggtggccta tgccgagtgc ctcaaagtgg aggagcgcgg
     9361 gtcccagcgc gtggacgcgg cgatcgcacg cggcgacgcg ctccgggagc tcggccgcta
     9421 ccccgaggcg ctccagt
//
LOCUS       DASTKC010000193        15513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1577814, whole genome shotgun sequence.
ACCESSION   DASTKC010000193 DASTKC010000000
VERSION     DASTKC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15513
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577814"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFR25_07940"
     CDS             <1..218
                     /locus_tag="VFR25_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9941019.1"
                     /translation="YCDIHSNMEAFIVVLPSAWFAQPDAEGRFTLPPLPAGTYKVRVW
                     HPDLGDRTVEVTVSDDQTRDVELSFLQ"
     gene            215..2059
                     /locus_tag="VFR25_07945"
     CDS             215..2059
                     /locus_tag="VFR25_07945"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941020.1"
                     /translation="MKGVRLHWKILFATVLPLLALTAAVLWTVNRSITDQAERNVRED
                     LLRAASIFEDMLGTRAEELAIAGQVIVQDPKFFSILTLPGSASDPQVRATAEGVAADF
                     NGITQADLFVVYDANGRVVADASRRRLAAPAPQGAALASDDAHPRSAILVEGGRHFQV
                     AAIPVLAGPRRVGSLLLGAAIGQDLAERLRTLTRSEVTFVSNGAVTGSTLEASADRHA
                     LARELYSRGIHGHSDPDRGPTEASVFTFVGGTSRYITVAGAIRGSAPGEGQLFAIQRS
                     LAAETAFLSGMRTALLELGLLGAVLSLALGYVISRSITAPVRTLVRGAEEMERGNYDY
                     PLQVKSMDEIGYLAVRFRDMRQKQRVYVKSLEEVARLKGDFIDVASHELRTPLSVLKG
                     FLELLSHERLGPLTPNQKEAVAAMEGGVSALTRIAEDAWRVSQIQSRRLVLATGSHDI
                     GSILEEAIGRARVAARGRKVEIDMERPSSIPPLLVDGPRLTEAVAHLVSNGVRFTPDG
                     GSVTASAVVEDGDLLIHVADTGEGIPSERLEKLLERSFAFRDALNHHSSSTLEYKSEG
                     LGLGIPIARGVAEAHGGALTARSRPGAGSVFTLRIPAKSAFESEEEAA"
     gene            2056..3234
                     /locus_tag="VFR25_07950"
     CDS             2056..3234
                     /locus_tag="VFR25_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941021.1"
                     /translation="MTPRVPTVLTVLALAACALAPTPGRAQLPDVHVRGLLDLVAAQR
                     SDTHELNTLSRGDSPYDPYGLRLFVDGNVNEHVDFFGQVVLRDGATPYVDGAYVLFTP
                     FHAYDTHLMAGKIPWPIGTYGPRTYSNKNPLIATPLMYQYHTTLLWYALPPSADVLIA
                     NSGNGQYGVDYFGYPMSKGMAVVDDSWWDVGISAVGSTGPVECSMGITAGTPGWGSTV
                     KDENSGKTIMGRIGLAPTPGLRFGVSGALGPYLVEGLNGELGPGQTVDGFYQTLGMVD
                     AEFATGYFELRGEGAWNTWQTPTVGDLGVTTGYVEMKYHLPFGAYAAGRLDGMRFSKI
                     EDSGGVEHPWDSNIARWELGAGYRVDRNILMKLVYQHTTMEGRDIKPSLFAAQGSFTF
                     "
     gene            3278..4636
                     /locus_tag="VFR25_07955"
     CDS             3278..4636
                     /locus_tag="VFR25_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9941022.1"
                     /translation="MADLNAVDHWLETHLDDSLSELRRLVAQPSVVAQNRGIPECAEL
                     VASMMKARGFATEVLPTGGSPVVVAERGGRSDRTLVIYNHYDVQPPEPLELWTSPPFE
                     ATLRDGKLYGRGVSDDKGHLVARLHAIDALLAAGGELPCRIKFVVEGEEEVGSVHMPA
                     FLEREKGRLQGDACLWEFGYLDQGDRPLLHAGLRGICYVELRVRTAAHDAHSGIGGSI
                     FPNAAWRLTWALASLKGPDERIRIPGHYDKVRAPSARDRELMAKARDRALEFRELYGV
                     RGFLRGLEGEDLKIAEVFEPTCTICGITTGYQGPGSKTVLPAYASAKVDFRLVPDMMP
                     EDVFRSLRAHLDAQGFQDVETVLLGGTGPGRTDPDHPFLALVARTAEPVYGAPSQIVP
                     MIGGSGPTHSFIHILGLPIATAGLGYADTRAHAPDENLRVEHYLKHSKHVVRILEAFA
                     QS"
     gene            4789..5277
                     /locus_tag="VFR25_07960"
     CDS             4789..5277
                     /locus_tag="VFR25_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET9941023.1"
                     /translation="MVVCATAVLLLGCNAKDYQVATVAPTSGNTAKGEVRFYKVEGGV
                     RVIASIEGLTPGKHGFHVHEKGDCSAPDAASAGGHFNPTGSPHGAPTAPPTGRHAGDL
                     GNLEADSNGKANYDRVDSMLVYESLSGLSILVHAGEDDFVTQPSGNSGARVGCGVIHP
                     AQ"
     gene            complement(5291..5545)
                     /locus_tag="VFR25_07965"
     CDS             complement(5291..5545)
                     /locus_tag="VFR25_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941024.1"
                     /translation="MARCECCGNEYDKSFDVILDGKSHTFDSFECAIHILAPTCEHCD
                     CRIIGHGVESGGALYCCVSCARQTGVSGLRDRAEAGPMAH"
     gene            5787..7106
                     /locus_tag="VFR25_07970"
     CDS             5787..7106
                     /locus_tag="VFR25_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9941025.1"
                     /translation="MNLLATRRGRLAAFFLLYVTEGIPSGFTGVAVATRMRRLGLDPA
                     AIGMFTAALLLPWAVKWAFGPFVDVISSDRWGRRRSWILGTQTLMSLTILLAMGVDMR
                     ASLTLFTAVLVAHNLCSAVQDVAIDALAVQVLPGDERGLANGLMFGGQYLGSALGGTG
                     ALFLASVAGFPVTFIAVSAAIALVTAAVAWPMREPPGPPRPPLGLRSVGQGIVAFVRD
                     TARSFTGSRAALAAIVLALLPMGGYALSLSLQSNLAVELGLTDRQVGTLSLLSTLLSA
                     GFCVLGGWISDRWGRRRSLATFIIGTTLPTFAMAAAMQHFHWILPVSPLLPNRPVPAP
                     ELVSLFWSLTLAYAVFQGLMYGVGTAIFMDVTNPLVAATQFTGYMALCNLVYSYSAAW
                     QGHAAVAWGYPATLVIDGAFGLLSVLVLPLMGSLAGSRAPASAPEAS"
     gene            7182..7658
                     /locus_tag="VFR25_07975"
     CDS             7182..7658
                     /locus_tag="VFR25_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941026.1"
                     /translation="MHHSFRRALVHARCSHRGPARHSFSLHASPWGHADARDAAADAG
                     GGGFGVRRPLRFLAYKLGLAEPQVVQLARILDELKTERAQHAVDERRRLSALAEAVGS
                     ESFVEAKAEEAASLKSASTQRLEAAVVKAVRQLHELLDTAQRERLAYLIRTGTLVL"
     gene            7807..8409
                     /locus_tag="VFR25_07980"
     CDS             7807..8409
                     /locus_tag="VFR25_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9941027.1"
                     /translation="MAASRRAISDRHQLRALASPVRQELVDLLARTGPTSAAELGALI
                     GRPADGLYYHLRELQRVGLISPAGSRKRGRRREAIFRSAYREPSLVHDPSPKGNSPAV
                     TTIIGSMLRLGARDFRKAVGLPRVRTHGPNRELWGLRVTGWLTPDEVVEVNRRIVSLQ
                     NAVARPRSRGKLYAITILLTPLEHRARKKQRRPRVPRTNP"
     gene            8566..9528
                     /locus_tag="VFR25_07985"
     CDS             8566..9528
                     /locus_tag="VFR25_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9941028.1"
                     /translation="MLSTRGLVKVYPGPVTALNGVDLDVAPGMFGLLGPNGAGKSTFM
                     KILAGLLEPTSGSVTLDGADTLSHPETLWPRLGYLPQEFGFYPHLSGQAMLAHLLELK
                     GVTAPQGMKKLTAELLERVNLSHAAKRAVKGYSGGMRQRLGIAQAIAGNPRLIIVDEP
                     TAGLDPEERLRFYHLLSELAQDRIVILSTHIVEDVAVLCPRFAVIRSGRLLAVTTPSE
                     ARSAIAGCMYEGTVEVADLPRLRAERVVTQALLVEGKNKARLYEPSGSFPEGFAPVPV
                     TLEDAYLVLMRLGSLPSRDRTGTSIHRGDGVPGVITTAPAGGER"
     gene            9525..13133
                     /locus_tag="VFR25_07990"
     CDS             9525..13133
                     /locus_tag="VFR25_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9941029.1"
                     /translation="MSLRRFGATFGVELSHTFRRPLFIILALILLLSGWGLSTGHVQI
                     SSGDSSVGGTKAWITSEFAQTQMMTYIILLYYAFFIAIGAGLTLIRDRETKVDSLLYS
                     TPLTAGEYVWGRYLAILVGFGVLLAWQVAVNAFFNHVVPNASANEIRGPFSLASYALP
                     VLTIGLPFLVFYAGLAMYVGQRTQNGILVFFMSVALLLVCGFFLWTWSPSWLDWRINR
                     LLQVLDPSGYRWLNEALLKVDRGVAYYNKAHVAYDGLFWINRLAMLAIGLGSVLLTQR
                     SVARAKRGISAARVKQAKDRAEAAAPALNPWPEGSQGLAGLAMATKRPSFLGTIRAVA
                     GAEVRELSRQKGLYVFIPLILIQALGNALIAVGAFDTPILLTPGITAVQLASQVSGWI
                     CLLLMFYTVESLERERAADLAPIVYSTPIRTGALLAGKALANALVGAAVLMASLLACF
                     IAVLVQHTVPFSVVPFALVWGLLLIPTFLAWTAFVTAIYAWVGNRYATYGIALAAIIF
                     TLFKALFQQISWAYNWPLWGALRWSDLGFFETDRTALLWNRIGVLGLAVFFTVLAVRL
                     FARRGPDAVQTIHRLSPKSLVGSMVKLSPYAAVPAIAVCVLMVQVHDGLNGGMAKKVG
                     KDYWAKNLKTWMNAPLPDIGRADISLKLDPAKHFIASKGTLTLVNGSDSTLARFPLSG
                     AQHWEHLAWTMNGKEYKPETTEHLYVIEPPHPLAPGDSVVVGWSFDGRYPKGVTKNGE
                     NTDEFILPSGVVLTGFTPSFMPVLGFLESVGESKDNKTEPRRYPRDYWKGITRAGFGA
                     TAWFPARIAVTGPEAYTLNSVGVCTSNTVKDGWRTQVWETTDHPVKILNVVCGRWKEK
                     QGHGTTIYYSAVHPYNVNEMSTTLDAARRYYSEWFLPYPWKELKLSEFPALAGYAQGF
                     GTNITFSENIGFLTRNDDKTDATFLVTAHEAAHQWWGNILTPADGPNGDFLSEGAAHF
                     STMLLFNQVKGPAARMEFAKGIESRYNDRRRVDDERAMYDVDGKREADNTVLYDRGGW
                     VFWMLYDYMGHDRALAGYRNFIQTWSVSRDHPALQDFVAAMRPYAQDPLAYDAFVKQW
                     MEDKVVPEYRVSDAKRLSARSGYSVTATVRNIGTGIMPVEIAAAAGERWTKAKDASGP
                     ASTPNPAYHDARTTITLGAGESRTVTLHCDFKPERVVVDPDVRVLQLRRKLAVATL"
     gene            complement(13101..14066)
                     /locus_tag="VFR25_07995"
     CDS             complement(13101..14066)
                     /locus_tag="VFR25_07995"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET9941030.1"
                     /translation="MRTRRLVSLCLALALAIPACGKREPEPPETETVEKEPMITLPVV
                     ESMTYLGLEEGVAVTLDHGRWVSDTAVPGSAARAVVALVRNFILIGDVDGDSHEDGLV
                     FLEGHSGRNTTNVYWALVQHRDDEIQNTAIRRIGDRVQIRDARFEHDRILLDVVEAGP
                     DDELDFPGQLQTRIWEWNSGTWQERGNPNPPERLSLDTIEGTRWRLRALNDSTPAPET
                     PEVTLSYSNGRFSGRGACNTYSAAVHQKKNPGEIGVSLPVATRMACADSVMRLESRFL
                     SLLPKADRFGFAATDLLLEYPSGDSRERLYFERIPSEGGDGQLSP"
     gene            14208..14711
                     /locus_tag="VFR25_08000"
     CDS             14208..14711
                     /locus_tag="VFR25_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941031.1"
                     /translation="MTELDASDPSIRAFLRHTLATLAYRAGKTVRGTPEEFGRFRATP
                     QSTSVVEVLAHMGDLMDWALRTAEGKPSFTQATPLPWEEEIRRFFASLGALDAYLASG
                     APIHYEPARLFQGALADALTHTGQLAMLRRLSGFKMKGESYNRADIRIGRVGLDQTPP
                     DPKFEFD"
     gene            complement(14823..15413)
                     /locus_tag="VFR25_08005"
     CDS             complement(14823..15413)
                     /locus_tag="VFR25_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941032.1"
                     /translation="MNRRTVWSLALAALVTAFMAVDASARTIVPTPNLSGTWRLDQRH
                     SDKNFGRGFGNNAALALPRAIQITQGPNRLRVTNRRGDVVQVVLLDDHFGFGGRDRFG
                     NNGRYDNNEQYGNDGRFGNNDNDWNGYRNQSGRDVLVGQWHGPMLVAEQTGARGTQVT
                     QTFRLANGGRTLVVRTEVARGRFGTTMQIDQVFQRA"
BASE COUNT         2384 a         5368 c         5182 g         2579 t
ORIGIN      
        1 tctactgcga catccactcc aacatggaag cgttcatcgt cgtcctgccc agcgcgtggt
       61 tcgcgcaacc cgacgccgag ggccgcttca cgctgccgcc gctgccggcg gggacgtaca
      121 aggtccgcgt gtggcatccg gacctcggtg atcgcacggt cgaagtcacc gtctcggatg
      181 accagacgcg cgacgtcgag ctgagcttcc ttcaatgaag ggcgtgcggc tccactggaa
      241 gatcctcttc gcgaccgttc tgccgctcct cgctttgacc gcggccgtgc tctggaccgt
      301 gaaccgaagc atcacggatc aggcggaacg gaacgttcgc gaggacctgc tccgcgcggc
      361 ctcgatcttc gaggacatgc tcgggacgcg cgcggaggag ctcgccatcg cggggcaggt
      421 catcgtgcag gatccgaagt tcttctccat cctcaccctt cccgggtcgg cctccgaccc
      481 tcaggtcagg gccaccgccg agggcgtggc ggccgacttc aacggcatca cgcaggccga
      541 tctcttcgtc gtctacgacg cgaacggacg cgtcgtggcg gacgcgagcc gccgtcgtct
      601 cgccgcgccg gctccccagg gagcggccct ggcttccgat gacgcccacc cccgctcggc
      661 gatcctggtc gaaggaggac gccacttcca ggtggccgcc attccggtcc tggcgggtcc
      721 gaggcgagtg ggatcgctcc tgctcggcgc cgcgatcggt caggacctgg ccgagcgcct
      781 tcgcaccttg acccggagcg aggtgacgtt cgtctcgaac ggcgccgtca cgggatccac
      841 cctcgaggcg agcgccgacc ggcacgcgct cgcgagggag ctctactcgc gcggcatcca
      901 cggccactcc gatccggacc gaggtcccac ggaggcctca gtcttcacgt tcgtgggcgg
      961 gacctcccgc tacatcacgg tcgcgggagc cattcgcggc tcggcgccgg gcgaggggca
     1021 gctcttcgcc atccagcggt cgctcgccgc cgagacggcg ttcctctccg gcatgcgcac
     1081 ggccctcctc gagctcgggc tcctcggcgc cgtgctctcg ctcgcgctcg gctacgtgat
     1141 ctcgcggagc atcaccgccc cggtgcgcac gctcgtgcgc ggcgcggagg agatggagcg
     1201 gggcaactac gactatccgc tccaggtgaa gagcatggac gagatcggat acctcgccgt
     1261 gcgattccgc gacatgcgcc agaagcagcg ggtctacgtg aagagcctcg aggaagtcgc
     1321 caggttgaag ggggacttca tcgacgtcgc gtcgcacgag ctccgcaccc cgctgagcgt
     1381 gctcaagggc tttctcgagc tcctctccca cgagcggctc ggcccgctga cgccgaacca
     1441 gaaggaggcg gtcgcggcga tggagggagg cgtttccgcg ctcacaagga ttgcggagga
     1501 cgcatggcgc gtctcccaga tccagagccg ccgcctggtc ctcgcgacag ggtcgcacga
     1561 catcggctcg atcctggagg aagcgatcgg gcgcgcacgg gtcgcggcgc gcggacggaa
     1621 ggtcgagatc gacatggagc gcccgtcctc gatccccccg ctcctcgtcg acggaccgcg
     1681 cctgaccgag gccgtggcgc acctcgtttc gaacggcgtg cggttcacac ccgacggagg
     1741 gagcgtgacc gcctcggccg tggtggagga cggcgatctc ctgatccacg tcgccgatac
     1801 gggggagggc attccctcgg agcgtctgga gaagctgctc gagcggagct tcgcgtttcg
     1861 cgacgcgctg aaccaccact cgtcgtcgac gctcgaatac aaatccgagg ggctgggcct
     1921 cgggatcccg atcgcgcgcg gcgtcgcgga agcacacggc ggcgcgctca cggcgcggag
     1981 ccgccccggc gcgggctccg tattcacgct ccggatcccg gccaagtcgg cgttcgagag
     2041 cgaggaggag gcggcgtgac cccgcgcgtc ccgacggttc tcacggtcct ggcgctcgcg
     2101 gcgtgcgcgc tcgcgcccac tcctggccgg gcgcagctgc ccgacgtcca cgtgcgcggg
     2161 cttctggatc tggtcgcggc gcagcgcagc gacacacacg agctcaacac gctctctcgc
     2221 ggtgattcgc cctacgatcc ctacgggctg cggctcttcg tcgacggcaa cgtgaacgag
     2281 cacgtcgact tcttcggaca ggtcgtcctg cgcgatgggg cgaccccgta cgtggacgga
     2341 gcctatgtcc tcttcacgcc gtttcacgcg tacgacacgc acctcatggc cggcaagatt
     2401 ccgtggccca tcggcaccta tgggccgcgc acctactcga acaagaaccc cctcatcgcg
     2461 actccgctca tgtaccagta ccacacgacc ctcttgtggt atgcgctccc gcccagcgcc
     2521 gacgtactga tcgcgaattc aggaaacggt cagtacggcg tcgactactt cggctatccc
     2581 atgagcaagg gcatggccgt ggtggacgac tcctggtggg acgtcggcat cagtgcggtg
     2641 ggcagcacgg gcccggtcga gtgctcgatg gggatcaccg ccggtacgcc gggctggggg
     2701 agcaccgtga aggacgagaa ctcgggcaag acgatcatgg ggcggatcgg tctcgctccg
     2761 acgccaggct tgcgcttcgg cgtctcgggg gcgctcgggc cctatctcgt cgagggtttg
     2821 aacggcgagc ttggtcccgg ccagaccgtg gacgggttct atcagacgct cggcatggtg
     2881 gacgctgaat tcgcgacggg ctacttcgag ctgcgcggtg agggcgcgtg gaacacctgg
     2941 cagaccccga cggtggggga tctgggcgtc acgacgggat acgtggagat gaagtaccac
     3001 cttcccttcg gcgcctacgc cgcggggcgt ctcgacggca tgcgcttctc gaagatcgag
     3061 gactcgggcg gagtcgagca cccctgggac tcgaacatcg cgcgctggga gctcggcgcc
     3121 ggttaccgcg tcgaccgcaa catcctcatg aagctggtct accagcacac gaccatggaa
     3181 gggcgtgaca tcaagccgtc gctctttgcg gcgcagggtt ccttcacgtt ctagttcgcc
     3241 gcttgcggtt ccgcgcaccg ccccgtagat tcgcccgatg gccgacctca acgccgtgga
     3301 ccactggctc gagacccacc tcgatgacag cctgagcgag cttcgccggc tcgtcgcgca
     3361 gcccagcgtg gtcgcgcaaa accgcggaat cccggagtgc gcggagctgg tcgcctcgat
     3421 gatgaaggcg cgcggcttcg cgaccgaggt gctcccgacc ggcggatcgc cggtcgtggt
     3481 cgccgagcgc ggcggccgga gcgaccgcac gctcgtgatc tacaaccact acgacgtgca
     3541 gccccccgag cccctcgaac tctggacgag ccctcccttc gaagccacgc tccgggacgg
     3601 caagctctac gggcgcggcg tgagcgacga caagggacac ctcgtcgcac ggctccacgc
     3661 gatcgacgcg ctcctcgccg caggggggga gcttccgtgc cggatcaagt tcgtggtcga
     3721 aggggaagag gaggtcggaa gcgtccacat gccggcgttc ctcgagcgcg agaagggccg
     3781 gctccagggc gacgcatgcc tctgggagtt cggatatctg gatcaggggg accggccgct
     3841 gctccacgcc gggctgcgcg ggatctgcta cgtcgagctc cgcgtgcgca ccgccgcgca
     3901 cgacgcgcac tcgggcatcg gtggatcgat cttcccgaac gccgcgtgga ggctcacgtg
     3961 ggcgctcgcg tcgctcaagg gccctgacga gcgaatccgc attccggggc attacgacaa
     4021 ggtgcgcgct ccctcggcgc gcgaccgcga gctcatggcg aaggcgcggg atcgggcgct
     4081 ggaattccgt gagctctacg gcgtgcgggg cttcctccgc ggtctcgagg gagaggacct
     4141 caagatcgcc gaggtgttcg agcccacctg cacgatctgc gggatcacga cgggatatca
     4201 ggggcccggc tcgaagacgg tgctccccgc gtacgcgtcg gcaaaggtgg acttccggct
     4261 cgtgcccgac atgatgcccg aggacgtgtt ccgctccctg cgcgcgcatc tggacgcgca
     4321 aggcttccag gacgtcgaga cggtcctcct cggtggaacc ggtccggggc gcaccgatcc
     4381 ggaccatccg ttcctcgccc tcgtggcacg caccgcggag cccgtgtacg gcgcgccctc
     4441 gcagatcgtg ccgatgatcg gcggctcggg gccgacccat tccttcatcc acatcctcgg
     4501 cctgccgatc gcgaccgcgg ggcttggata cgcggacacg cgcgcgcacg cgccggacga
     4561 gaacctccgc gtcgagcact acctgaagca ttcgaagcac gtggttcgaa tcctcgaggc
     4621 cttcgcccag agctgacgcg gcgctcgccc ctctagggat tgccgcggtg ccgcgcagcc
     4681 ggtagggtgt aggcgaggga cggagagcgg aggacgccgg aacgcgcgtc gctcccacgg
     4741 gtccacaagg aggggatgat gggatcgagg ctccgaatcg tagaggccgt ggttgtctgc
     4801 gccacggccg tcttgcttct aggatgcaac gcgaaggact accaggtcgc cacggtcgct
     4861 ccgacctcgg gaaacacggc caagggcgag gtccgcttct acaaggtgga ggggggcgtg
     4921 cgcgtgatcg cgtcgatcga agggctcacg ccggggaagc acggattcca cgtgcacgag
     4981 aagggggact gctccgcgcc ggacgccgcg tcggccggtg gacatttcaa tccgacgggc
     5041 tcgccgcacg gcgcgccgac cgcgcccccg acggggaggc acgcgggaga tctcggcaat
     5101 ctggaagccg attccaacgg caaggcgaac tacgaccgcg tcgactcgat gctcgtctac
     5161 gagagcttga gcggcctctc gatcctggtt cacgcgggcg aggacgactt cgtgacgcag
     5221 ccctccggca actcgggagc gcgcgtcggc tgcggggtga tccacccggc ccagtgagcc
     5281 gggccgccgc tcagtgcgcc atcggccccg cctcggcgcg atcgcggagc ccggagactc
     5341 cggtctgacg ggcgcagctc acgcagcagt agagcgcacc gccggactcc acgccgtgac
     5401 cgatgatccg gcagtcgcag tgctcgcagg tcggggcgag gatgtggatc gcgcactcga
     5461 agctgtcgaa ggtgtggctc ttcccatcca ggatcacgtc gaaggacttg tcgtactcgt
     5521 tgccgcagca ctcgcaccgc gccatgacct ctcctctccc cgcgcttggc ggaggtcgaa
     5581 actgcatttc gttgtaagac ccacggccgg gaaagcgtcg cgccctgccg tggaccgcta
     5641 ccgcgtcact tcaccctggg gccgtatccc caacgaaaat aatcggatcg ggcagcgcct
     5701 ctctcgagac atcggggccc gggacaggag cgggtgaagg gagcggcatg cttgcatagg
     5761 tcgggcctca cgcgtagtcc ggctgcatga acctcctcgc gaccaggcgc gggcggcttg
     5821 ccgcgttctt cctcctctac gtgaccgagg gcatcccgtc gggattcacc ggcgtcgctg
     5881 tcgccacgcg catgcggcgc ctggggctcg acccggcggc catcggcatg ttcacggcag
     5941 ctctcctcct cccttgggcc gtgaaatggg cgttcggtcc ctttgtcgac gtgatttcga
     6001 gcgaccgatg gggtcgccgt cgtagctgga tcctcgggac acagacgctc atgtcgctga
     6061 ccatcctgct cgcgatggga gtcgacatgc gcgcgagctt gacgctcttc accgcggtcc
     6121 tcgtcgcgca caatctctgc agcgcggtcc aggacgtcgc catcgacgcg ctcgcggtgc
     6181 aggtgcttcc gggcgacgag cgcgggctgg ccaacggact catgttcgga gggcagtatc
     6241 tgggctcggc gctcggagga accggcgcct tgttcctggc ttcggtcgcc ggctttcccg
     6301 tcaccttcat cgcggtgtcg gccgccatcg cgctggtcac cgccgctgtc gcctggccga
     6361 tgcgcgagcc ccctggccct ccgcggccgc cgctcggtct gcgatcggta ggacagggca
     6421 tcgtcgcgtt cgtccgggac acggcccgct ccttcaccgg gagccgggcc gcgctcgccg
     6481 cgatcgtgct ggcgcttctc cccatgggcg gttacgcgtt gtcgctctcg ctccagtcga
     6541 acctcgcggt ggagctcggc ctgaccgacc gccaggttgg gacgctctcg ctcctgtcca
     6601 cgctcctctc ggcgggattc tgcgtgctcg gcggctggat ctcggaccga tggggacgac
     6661 gtcgctcgct tgcgacgttc atcatcggca ccacgctgcc gacattcgcg atggccgcgg
     6721 ccatgcagca cttccactgg atcctgccgg tgagcccgct cctcccgaac cgtcccgttc
     6781 cggcgcccga gctggtctcg ctcttctggt cgctcacgct cgcgtacgcc gtcttccagg
     6841 ggctcatgta tggcgtgggc accgccatct tcatggacgt gacgaatccg ctcgtggccg
     6901 cgacgcagtt cacgggctac atggcgctgt gcaacctggt ctactcctac agcgcggcct
     6961 ggcaagggca tgccgcggtc gcttggggtt atcccgcaac cctcgtgatc gatggcgcgt
     7021 tcggcctctt gagcgtcctc gtcctgccgc tcatgggctc tctcgccggg agccgagcac
     7081 ccgcatcggc gccggaggca tcctagctgg aagcgtgagg catggtgccc acgcgtcatg
     7141 aacccgccgg gagcgaacgc cccggcgcga ggaggtgccc gatgcaccat tcgttccgtc
     7201 gtgccctggt ccacgcccgc tgttcccacc gaggcccggc ccgccactcc ttttcgctcc
     7261 acgcatcacc ctggggtcac gccgacgcgc gcgacgccgc ggccgatgcc ggtggcggcg
     7321 gattcggagt ccgtcgtccc cttcgattcc tcgcctacaa gctcggcctc gcggagccgc
     7381 aggtcgtcca gctcgcccga atcctggacg agctcaagac cgagcgcgcg cagcacgcgg
     7441 tggacgagcg gcgtcgcctc tcggcgctcg ccgaagcggt ggggtcggag tcgttcgtcg
     7501 aggcgaaggc ggaggaggcc gcgtccttga agtctgcgag cacgcagcgc ctcgaggcgg
     7561 ccgtcgtgaa ggcggtccgc cagctccacg agctgctcga cacggcgcag cgcgagcggc
     7621 tcgcctatct catccgtacg ggtacgctcg tcctctaacg aggggtaccc aagccattcc
     7681 accgcggggc ggcacggcgc gagccggccg ccccgtttgg ctcgagccgg tggggccccg
     7741 aatcggcgcg tggctacaat tgaaaatact gattgatttt catcaatcgg ggcgtatcgt
     7801 ctggatgtgg ccgcatcgcg acgcgcgatc tccgatcgcc accagctccg cgccctggcc
     7861 agtcctgtcc gacaggagct ggtcgacctt ctcgcgcgta ccggtccgac ctctgccgca
     7921 gagttgggcg ccttgatcgg tcgccccgcc gacgggctct attaccacct gcgcgagctc
     7981 cagcgggtcg gtctcatctc gccggctgga tcgcgaaagc gggggcggcg ccgggaggcg
     8041 atctttcgct ccgcctaccg tgaaccgtcc ctcgtccatg atccgtcgcc caaaggcaat
     8101 tcaccggccg tgaccacgat catcggctcg atgctccgcc tcggggcgcg tgacttccgg
     8161 aaggctgtgg gcttaccccg ggttcgcacc cacggaccaa atcgagagct ttggggcctt
     8221 cgggtgacag gctggctcac gccggacgag gtcgtcgaag tgaaccggcg aatcgtctcg
     8281 ctccaaaacg ccgtcgcccg cccgcgctcc cggggaaagc tctacgcgat cacgatcttg
     8341 ctcacgccgc tcgagcatcg cgcgcgcaag aaacaacgcc gacccagggt gccccggacg
     8401 aatccatgat cgcgtgccca gaaccgctgc tgctctcgcg aaacactgaa ggccagcccg
     8461 gcgtagggga cggtgtcgag accgcgggag accgcgggac agacgggcac gtcttcctcg
     8521 gcacaccggg aggcgccgat ggcatcacaa accgaggagg atcccatgct tagcacgcgc
     8581 ggcctggtga aggtctaccc cggcccggtc accgccttga acggcgtcga tctcgacgtg
     8641 gcccccggaa tgttcggact cctcggtccg aacggtgcgg gaaagtcgac cttcatgaag
     8701 atcctggccg gcctgctcga gccgacctcc ggctccgtga ccctcgacgg tgccgacacc
     8761 ctgagccatc cagaaacact ctggccgagg ctcggctacc tgcctcagga gttcggcttc
     8821 tatccccact tgagcgggca ggcaatgctc gcgcacctcc tcgagctgaa gggcgtgacg
     8881 gcgccccagg gcatgaagaa gctcacggcc gagctgctcg agcgcgtcaa tctgagccac
     8941 gcggcgaagc gcgcggtgaa gggctactcg ggaggcatgc gccagcgcct cggcatcgcg
     9001 caggcgatcg cggggaatcc gcgcctcatc atcgtcgacg agcccacggc gggcctcgac
     9061 cccgaggagc gcctccgctt ctaccatctc ctctccgagc tcgcgcagga ccggatcgtg
     9121 atcctctcga cgcacatcgt cgaagacgtc gcggtcttgt gtccgcggtt cgccgtgatc
     9181 cgctcgggac ggttgctcgc ggtcacgacg ccgagcgagg cgcgctccgc gatcgcgggg
     9241 tgcatgtacg aggggacggt cgaggtggcg gacctgccga ggctccgcgc ggagcgcgtg
     9301 gtcacgcagg cgctcctcgt cgaagggaag aacaaggcgc gcctctacga gccgtcgggg
     9361 tcattcccgg agggtttcgc tccggtcccg gtgacgctcg aggacgccta cctcgtgctc
     9421 atgcgcctgg gctcgctgcc gtcgcgcgac cgaacgggca cgtccatcca ccggggcgac
     9481 ggcgtcccgg gggtgatcac gacggcgccg gcgggagggg agcgatgagc cttcgccgct
     9541 tcggcgcgac gttcggtgtc gagctcagtc acacgttccg ccgcccgctc ttcatcatcc
     9601 tggctctgat ccttctcctg agcggatggg ggctctccac gggacacgtc cagatctcct
     9661 cgggggacag ctcggtcggg gggacgaagg cgtggatcac gtccgagttc gcgcagacgc
     9721 agatgatgac ctacatcatc ctcctctatt acgccttctt catcgcgatc ggggccgggc
     9781 tcaccctgat ccgcgatcgc gagaccaagg tggactcgct cctctactcc acgccgctca
     9841 ccgccgggga gtacgtgtgg gggcggtatc tcgccatcct ggtcggattc ggcgtgctcc
     9901 tggcctggca ggtggcggtc aacgccttct tcaatcacgt cgtgcccaat gcgagcgcca
     9961 acgagatccg agggcctttc tcgctcgcga gctatgcgct ccccgtcctc acgatcgggc
    10021 tcccgttcct cgtcttctac gccggcctcg ccatgtacgt ggggcagcgg acgcagaacg
    10081 ggatcctcgt cttcttcatg tcggtcgcgc tgctgcttgt ctgcggcttc ttcctctgga
    10141 cctggtcccc ctcgtggctc gactggagga tcaaccggct cctgcaagtg ctcgaccctt
    10201 ccggataccg ctggctgaac gaggcgctcc tcaaggtcga ccggggcgtg gcctactaca
    10261 acaaggcgca cgtggcgtac gacggtctct tctggatcaa tcgcctggcg atgctcgcga
    10321 tcggcctcgg atcggtgctc ctgacccaac gctcggtggc ccgcgcgaag cgcgggatct
    10381 ccgcggcccg cgtgaagcag gcgaaggacc gggccgaggc ggccgcgccg gcgttgaacc
    10441 cgtggcctga aggctcccag ggtctcgccg ggctcgcgat ggcgaccaag cgaccttcct
    10501 tcctcggcac catccgcgcc gtggcgggcg ccgaggtgcg cgagctgtcg cggcagaagg
    10561 gactctacgt cttcataccg ctgatcttga tccaggcgct cggcaacgcg ctcattgcgg
    10621 tcggcgcgtt cgacacgccg atcctgctca caccggggat cacggcggtc cagctcgcga
    10681 gccaggtgtc gggatggatc tgcctcctcc tcatgttcta cacggtcgag tcgctcgagc
    10741 gggagcgcgc cgcagatctc gcgccgatcg tgtactccac gcccatccgc acgggtgcgc
    10801 tcctcgccgg caaggcgctg gcgaatgccc tcgtcggcgc cgcggtcctc atggcgtcgc
    10861 tcctcgcatg cttcatcgcc gtgctcgtgc agcacacggt gccgttctcg gtcgttccct
    10921 tcgcgctggt ctggggactc ctcctcatcc cgaccttcct cgcctggacc gcgttcgtca
    10981 cggcgatcta cgcgtgggtc ggaaaccgct acgccaccta cggcatcgca ctcgccgcga
    11041 tcatattcac gctcttcaag gctctgttcc agcagatcag ctgggcctac aactggccgc
    11101 tctggggcgc gctccgctgg agcgacctgg gcttcttcga aaccgaccgg acggcgctcc
    11161 tctggaaccg gattggcgtc ctcgggcttg cggtcttctt cacggtgctc gcggtgcgtc
    11221 tcttcgcccg ccgcggcccc gatgcggtgc agacgattca ccgcctctcg cccaagagcc
    11281 tcgtgggttc gatggtcaag ctctcgccct acgctgcggt ccccgcgatc gcggtgtgcg
    11341 tcctcatggt tcaggtccac gacggactca acggcggcat ggccaagaag gtcggcaagg
    11401 actactgggc gaagaacctc aagacctgga tgaacgcgcc gctgcccgac atcgggcgcg
    11461 cagacatctc cttgaagctg gatcccgcga agcacttcat cgcgagcaag ggcacgctca
    11521 ccctggtgaa cggcagcgat tccacgctcg ctcgcttccc gctctccggg gcgcagcact
    11581 gggagcatct ggcctggacg atgaacggta aggagtacaa gcccgagacc accgagcacc
    11641 tctatgtcat cgagccgccg catccgctcg cccccggaga cagcgtggtc gtggggtgga
    11701 gcttcgacgg gcgctacccg aagggcgtca cgaagaacgg cgagaacacg gacgagttca
    11761 tccttccgtc aggcgtcgtt ctcaccggct tcacgcccag cttcatgccg gttctcggat
    11821 tcctcgagag cgtcggagag tcgaaggaca acaagaccga gccgcgccgc tatccgcgcg
    11881 actactggaa ggggatcacg cgcgccggct tcggcgcgac ggcgtggttc ccggcgcgga
    11941 tcgccgtgac cggaccggag gcctacaccc tcaactcggt cggcgtctgc acgagcaaca
    12001 cggtgaagga cggctggcgc acgcaggtct gggagaccac cgatcacccc gtcaagatct
    12061 tgaacgtagt ctgcggacgg tggaaggaga agcaggggca cggcacgacg atctactaca
    12121 gcgccgtgca cccctacaac gtgaacgaga tgtcgaccac gctcgacgcc gcgcgccggt
    12181 actactcgga gtggttcctc ccgtatccct ggaaggagct caagctctcc gagttcccgg
    12241 ccctcgccgg ctacgcgcag ggcttcggca ccaacatcac ctttagcgag aacatcggat
    12301 tcctcacgcg gaacgacgac aagacggacg cgaccttcct cgtcacggcg cacgaagcgg
    12361 cgcaccagtg gtgggggaac atcctcactc cggctgacgg cccgaacggc gacttcctga
    12421 gcgagggggc ggcgcacttc tcgacgatgc tcctcttcaa ccaggtcaag gggccggccg
    12481 cgcggatgga gttcgcgaag gggatcgagt cgcgctacaa cgaccgccgg cgcgtggatg
    12541 acgagcgcgc catgtacgac gtggacggaa agcgggaagc ggacaatacg gtcctctacg
    12601 accggggcgg ctgggtcttc tggatgctct acgactacat gggccacgac cgcgcgctcg
    12661 cgggctaccg gaacttcatc cagacctgga gcgtgagccg cgaccatccg gcgctccagg
    12721 acttcgtcgc cgcgatgcgt ccctacgcgc aggatccgct cgcatacgac gcgttcgtga
    12781 agcagtggat ggaggacaag gtcgtgcccg agtaccgcgt cagcgacgcg aagcggctca
    12841 gtgccaggtc gggctactcg gtcaccgcga ccgtgcgcaa catcggcacg ggcatcatgc
    12901 cggtggagat cgccgccgcc gcgggcgagc gatggacgaa ggcgaaggat gcttcgggcc
    12961 cggcgtcgac gccgaatccc gcgtaccacg acgcccgcac cacgatcacg ctcggggcgg
    13021 gggagtcgcg caccgtcacg ctccactgcg acttcaagcc ggagcgcgtc gtggtcgatc
    13081 cggacgtacg cgtgcttcag ctacggcgaa agctggccgt cgccaccctc tgaggggatg
    13141 cgctcgaagt agagccgctc ccgcgagtcg cccgacgggt actcgaggag gagatccgtg
    13201 gcggcgaagc cgaagcggtc cgccttcggc aggagcgata ggaagcgaga ctccaggcgc
    13261 atgaccgagt cggcgcacgc catgcgcgtc gcgaccggaa gcgatacgcc gatctccccc
    13321 ggattctttt tctggtgcac ggcggccgag tacgtattgc aggcccctcg ccccgagaag
    13381 cgcccgttgg agtaggagag cgtcacctcg ggagtttccg gcgcgggtgt ggaatcgttc
    13441 agggcgcgaa gccgccaccg cgtcccctcg atcgtgtcga gagagagccg ttcgggcgga
    13501 ttcgggttcc cccgctcctg ccaggtgccc gagttccact cccagatgcg cgtctggagc
    13561 tggccgggaa agtcgagctc gtcgtcggga ccggcctcga cgacgtcgag caggattcgg
    13621 tcgtgctcga agcgagcgtc gcggatctgg acgcggtccc caatcctgcg tatcgcggtg
    13681 ttctggatct cgtcgtccct gtgctgcacc agggcccagt agacgttggt ggtgttcctg
    13741 cccgagtgcc cttcgaggaa gacgagcccg tcctcgtggc tgtctccgtc gacgtctccg
    13801 atcaggatga agttgcgcac gagcgccacg accgcccgcg cggcacttcc gggtacggcg
    13861 gtgtcgctga cccaccgccc gtgatcgagc gtgacggcca ccccttcctc gaggcccagg
    13921 tacgtcatgc tctcgacgac cgggagcgtg atcatcggct ccttctccac cgtttcggtc
    13981 tcgggtggtt cgggttcgcg cttcccgcat gcggggatcg cgagcgcgag cgcgaggcag
    14041 agggaaacga gccggcgggt gcgcacctgg ttttgcctcc tcgatcggga tgaacgcggg
    14101 ccggcggacg ccgagcccgg gggagcgtat ccagcgttgc cccgttcggc aagggcgcgt
    14161 aggctcggtc gacccgattc gccggttttg cctcttgggg gatggccatg accgagctcg
    14221 acgcttccga tcccagtatc cgcgccttcc tgagacacac gctcgccacg ctcgcgtacc
    14281 gcgccgggaa gaccgtgcgg gggactcccg aagagttcgg acgctttcga gcgactcccc
    14341 agtcgacctc ggtggtcgag gtcctcgcgc acatggggga cctgatggac tgggcgctcc
    14401 gcaccgcgga agggaagccg agcttcacgc aggcgacgcc gctcccttgg gaggaagaaa
    14461 tccggcgctt tttcgcctcg ctcggcgcgc tcgacgcgta cctggcatcg ggggcgccga
    14521 tccactacga gccggcgcgt ctgttccagg gcgccctcgc ggatgcgctg acccacacgg
    14581 ggcagctcgc catgctgcgg cgcctctcgg gattcaagat gaaaggggag agctacaacc
    14641 gcgcggacat ccggatcgga cgcgtgggcc tcgatcagac gccccccgat cccaagttcg
    14701 agttcgacta gagtcaggat tcgatacaaa agcgtgacgg cgccgtccgg gtggacggcg
    14761 ccgttcgcgt ctcaggccga accgcggccc cgggcggaag gcacgcccgt tcgttgtagt
    14821 cgttacgccc gctggaacac ctggtcgatc tgcatcgtcg tgccgaagcg gccccgggcg
    14881 acctcggtcc gcacgaccag cgtgcggccg ccattggcga gtcggaacgt ctgcgtcacc
    14941 tgcgtgccgc gcgctcccgt ctgctcggct acgagcatgg gcccatgcca ctggccgacg
    15001 aggacgtcgc gaccgctctg gttgcggtag ccgttccaat cgttgtcgtt gttgccgaag
    15061 cgaccgtcgt tgccgtactg ctcgttgttg tcgtaccgcc cgttgttgcc gaaccgatcg
    15121 cgcccgccga atccgaagtg gtcgtcgagc aggaccacct ggaccacgtc accccggcgg
    15181 ttcgtcacgc ggaggcggtt cgggccctga gtgatctgga tcgctcgggg gagcgccagg
    15241 gcggcgttgt tgccgaagcc cctgccgaag ttcttgtcgc tgtgacgctg atcgagacgc
    15301 cacgtgccgc tcaagttcgg cgtggggacg atggtgcggg ccgacgcgtc gaccgccatg
    15361 aaagcagtga cgagagccgc cagcgcgaga ctccaaaccg tgcgtcgatt catggtcgtt
    15421 tcctcgggtt ctcaccctgg ggggtcagtg gccaccgaag ccttgcgccc cggcctgccc
    15481 gacacgagcc ccctgcccgg tgtctgaccc taa
//
LOCUS       DASTKC010000194         5463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2051933, whole genome shotgun sequence.
ACCESSION   DASTKC010000194 DASTKC010000000
VERSION     DASTKC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051933"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1199
                     /locus_tag="VFR25_08010"
     CDS             156..1199
                     /locus_tag="VFR25_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941033.1"
                     /translation="MRSRTTPRLASAAACVVVLVFLGGCSGRGGEATRSAIAREGTSG
                     THHFRLVSALEPSRVTLGQPTRWTLRAELPKEARAESLLRDPGAPTLDVTDRKSSTAR
                     GERGLIWSGAFEVRGFDLGRIPLPGMALVVRFPDGKDTLAFPVDTLAVDSLTEASSGS
                     LEPNRGPIKPELRPLDIALVAALGLLIVALIVAIVLAIRKRRRRAKGLEDEAPPEAPE
                     APFLRAIETLRGEIETLPRDRFYDRLSLAVRAYVGAVTGIPALDRTTSELKRELERRP
                     EVRDEAAALVESALRRSDLAKFARHEDPFAEARDALDRAASLAGKVTLAQAATPAPPT
                     SAPDEPSAARGAG"
     gene            1201..2187
                     /locus_tag="VFR25_08015"
     CDS             1201..2187
                     /locus_tag="VFR25_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9941034.1"
                     /translation="MRFQEPWFLLLLIPYAIVWIVLRRRGRFRAPVLPVAALATLPRP
                     GGVRVLVARIAPVLLPLATLLFCLAAARPQAGREMRQVVSEGIDIVLAIDQSGSMRCE
                     DFRPQNRLYVAKQTAKQFLRGRTQDRIALVGFAGRSELISPLTLDYEGLASLIDGLDF
                     GQLPDGTAVGGAIAQGAYVLRSAKGKSRVMILLTDGINNAGPVDPVTAARLAAAVGVR
                     VYAVGAGTLGQAPYPIDDPVLGRHYVWVRSDVDEPTLKQVASLTGGRYFRATNAELLS
                     RVYRDIDAMEPSQVEMRSYTRWAEVGPGLMGAAGALLALDLLIGAVLLRRYP"
     gene            2197..3198
                     /locus_tag="VFR25_08020"
     CDS             2197..3198
                     /locus_tag="VFR25_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9941035.1"
                     /translation="MRWADPDRLQLLWLVPLLLLAWGWALRARRRTEAALGDRDALRK
                     LTGDPGRGHRVLRGILLIAAAALAAVGLARPQSGYRLVTTTSTGADVVIALDLSRSMM
                     ARDAHPDRLGAAVREIHTILASLEGSSVGLIGFAGTGRLLSPLSTDYDGLATIVETLG
                     PDDIDLPGSDIGAGLALAARYLRRPSDRPRAVILISDGENLSGDPRAGASAVRQAGAR
                     LFAIGIGGAAGATIPVVDSTGAVVGQRRGLDGAPVRTKLDEGLLRDLVRRGGGRYEHG
                     DGSGQAAVRTADGVRSGGNQEVRGQTIRAYDDRFPWFAALAGTLLLVERAIPRRRRA"
     gene            3195..3938
                     /locus_tag="VFR25_08025"
     CDS             3195..3938
                     /locus_tag="VFR25_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941036.1"
                     /translation="MRAPRSLGAGLVRAAGLAPWLLLALGASGAVYEWGGAAGRGMRQ
                     LKEKHPKEAAQSFREAERELPRSAAVRYDRALALGAAGQADSAREAYRETFTSRDLRG
                     NPARSAAAYNLGNESLQQGRYDEAAKLYRQSLTIDPNRADAKKNLEEAIRRGRNRPPQ
                     ASSGGQGQGGEGSSGSGTSGQSPPGSQPNTPPPTRPPGSAPQPPGQMSGTPSPSEAEH
                     WLNALEEERKSARRQERAGSPQETKDKDW"
     gene            3940..>5463
                     /locus_tag="VFR25_08030"
     CDS             3940..>5463
                     /locus_tag="VFR25_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HET9941037.1"
                     /translation="MVNGGQRSAVLAPTFLLALAAALALLPSSARAEQLTAEAELDRQ
                     QVLVGEPATLAITITAEGLDVPGIKLPLIAGLDVTATEQSQGFSWMNGVISRSVTSTF
                     VLRANAPGDYVIPSFLIHSGKKEAKTQELVLHVRATMPRSGGGLGGEGGPELFARLTL
                     DRTRVYWNEGVTARFTLYSRVRYEGAPAWDPPDAPGFWTEVLGPARTGRIRVGDVEYD
                     ATEMRVEYFPTRTGKLRIGPGRVHAQVVRQVPVPDPWSALGMPETQVQDIELTTEQLA
                     VDVVPLPKGAPSTFRGAVGSYDLDVTVDRAVVRAREPVNVATVLKGEGNLAAASDPDV
                     SSTLPSRRYAGAASTGLDRSGLRLKGERRRETSFVPERQGSFAILPVRFSWFDPEQGR
                     YRTQVSDTITVRVLPPGSGSDTLGPAAVLGPVAPRRAHPGAGGSLALVPPWPADVVAW
                     GSLLAYLGVLTGLGVRDRSLRDPRRKRERELETIARELARAGTAAPTPARTAVLLRRA
                     "
BASE COUNT          749 a         1939 c         2009 g          766 t
ORIGIN      
        1 gccaggagtg gcgcgcggcc caatcgcgcc gctcgcggcg actgggcgag gcgttcatcc
       61 gcgggcgggc ggggcgcatc gcgctctcga cggacgcgcc ggtcgcaccc gcgctgatcc
      121 gtcacttcga agcccgcacc cgtcggaggt agcccgtgcg ctcccgcacc accccacggc
      181 tcgcgtcggc cgcggcgtgt gtcgtcgtcc tcgtcttcct cggtggatgc tccggtaggg
      241 gaggagaggc gacccgctcc gcgatcgcgc gcgaggggac gagcggcacg caccacttcc
      301 gcctcgtcag cgcgctcgag ccgtcgcgcg tgacgctggg ccagccgacg cgatggacgc
      361 tccgcgcgga gcttcccaag gaagcccgcg cggagagtct tctgcgcgat ccgggagcgc
      421 cgaccctcga cgtcaccgat cggaagtcgt cgacggcacg cggagagagg ggcctgatct
      481 ggtcgggagc gttcgaagtg cgaggcttcg atctgggtcg catcccgctc ccggggatgg
      541 cactcgtcgt gcgcttcccg gacgggaagg acacgctcgc gttccccgtg gacacgctcg
      601 cggtggactc gctcaccgag gcttcatccg ggtcgctcga acccaaccgc ggtcccatca
      661 agccggagct tcgcccgctc gacatcgcgc tcgtggcggc gctcggactc ctgatcgtgg
      721 ctctcatcgt cgcgatcgtc ctcgcgatcc ggaagcggcg gcgcagggcg aagggactgg
      781 aggacgaggc tccgcccgaa gctccggagg cgccgttcct ccgcgcgatc gagacgctcc
      841 gcggtgagat cgaaaccctg ccccgcgatc gtttctacga ccggctctcg ctggcggttc
      901 gcgcctatgt gggggcggtg accgggatcc ccgcgctcga ccggacgacg agcgagctga
      961 agcgcgagct cgaacggcgg ccggaggtgc gcgacgaggc agcggcgctc gtcgagagcg
     1021 ctctccggcg gagcgatctg gcgaagtttg cgcggcacga ggatcccttc gccgaagccc
     1081 gggacgcgct ggatcgggcg gcctcgctcg cggggaaggt gaccctggcg caggccgcga
     1141 cgcccgcgcc accaacgtcc gcgcccgacg agccttccgc ggcgcgagga gcgggctgac
     1201 atgcgctttc aggagccctg gttcctcctt ctcctcatcc cgtacgcgat cgtgtggatc
     1261 gtgctccgcc gccgcggccg cttccgcgcg ccggtcctgc cggtcgccgc gctcgcgacg
     1321 ctgccgcgtc cggggggagt gcgcgtgctc gtggcgcgca tcgcgcccgt gctccttccg
     1381 ctcgccacgc tccttttctg cctcgccgcc gcacgccccc aggcgggacg cgagatgcgc
     1441 caggtggtga gcgaggggat cgacatcgtg ctcgccatcg atcaatcggg gagcatgcgg
     1501 tgcgaagatt tccgtccgca gaaccggctc tacgtggcga agcagacggc caagcaattc
     1561 ctgcgcggcc gcacgcagga tcggatcgcg ctcgtcggat tcgcgggacg gagcgagctc
     1621 atctcgcctt tgaccctcga ctacgagggg ctcgcgagtc tgatcgacgg gctcgacttc
     1681 ggacagctgc cggacggcac cgcggtgggc ggcgcgatcg cgcagggcgc gtacgttctc
     1741 cggtccgcga aggggaagag ccgcgtcatg atcctgctga ccgacgggat caacaacgcc
     1801 ggtcccgtcg acccggtgac ggcggcgcgt ctcgcggcgg cggtcggcgt gcgggtctac
     1861 gcggtcggcg cgggcaccct ggggcaggca ccgtatccga tcgacgatcc cgtgctgggg
     1921 cgccactacg tgtgggtgcg ctccgacgtc gacgagccga cgctcaagca ggtggcgtcg
     1981 ctgactggcg gccgctactt ccgggcgacc aatgccgagc tcctctcgcg cgtctaccgc
     2041 gacatcgacg ccatggaacc ctcccaggtc gagatgcgtt cgtacacgcg atgggccgag
     2101 gtgggccctg gcctcatggg agcggcaggg gcgctcctcg cactggacct tctcatcggc
     2161 gcggtcctcc tgcgccggta tccgtagagg gctcgcatgc gctgggcgga tcccgatcga
     2221 ctccagctcc tctggctcgt cccgctcctc cttctggcgt ggggctgggc gctccgcgcg
     2281 cgccggagaa ccgaagccgc gctcggcgac agggacgcgc tccgcaagct gacgggcgat
     2341 cccgggcgcg gtcatcgcgt gcttcgcggc atcctcctga tcgccgcggc cgcgctggcc
     2401 gcggtcggcc tcgcccggcc gcaatcgggc tatcgcctcg tcacgacgac gtcgaccggc
     2461 gcggacgtcg tgatcgcgct cgatctctcg cgcagcatga tggcgcgcga cgcccacccg
     2521 gaccggctcg gggccgcggt ccgcgagatc cacacgatcc tcgcgtcgct cgagggatcg
     2581 tcggtcggac tgatcggctt cgccgggacg gggcgtctcc tgagcccgct ttccacggac
     2641 tacgacggac tcgcgacgat cgtcgagacc ctgggccccg acgacatcga tcttcccgga
     2701 agcgacatcg gcgccggcct cgcgctcgcg gcgcgctatc tccgacgccc gagcgaccgg
     2761 ccgcgcgcgg tgatcctgat ctcagacggc gaaaatctga gcggcgaccc gcgcgcgggc
     2821 gcgtccgcgg tgcgccaggc gggcgcacgc ctcttcgcga tcgggatcgg cggtgcggcc
     2881 ggagctacga tcccggtggt cgactcgaca ggagcggttg tcggacagcg gcgcggcttg
     2941 gacggggcgc cggtgcggac gaagctcgac gaaggactcc ttcgcgatct ggtccggcgc
     3001 gggggaggac ggtacgagca tggggacggg agcggacagg cggcggtgcg cacggcggac
     3061 ggggtgcgct cgggcggaaa tcaggaagtg cgcgggcaga cgatccgggc gtacgacgat
     3121 cggttcccgt ggtttgccgc gctggcgggg acgctccttc tcgtcgagcg cgcgattccg
     3181 aggcggaggc gcgcgtgaga gcgccgcgct ccctaggcgc cggtttggtg cgagccgccg
     3241 ggctcgcccc gtggctcctc ctcgcgctcg gcgcctcggg cgccgtgtac gagtggggcg
     3301 gcgcggcggg acgcggcatg cggcagctca aggagaagca ccctaaggaa gcggcgcagt
     3361 ctttccgcga agccgagcga gagcttccgc gctcggcggc cgtgcggtac gaccgggcgc
     3421 tcgcgctcgg ggcggccggg caggcggact ccgcgcgcga ggcgtacagg gagaccttca
     3481 cgtcgcgcga tctccgcgga aaccctgcgc gctcggcggc cgcgtacaat ctggggaacg
     3541 agagcctcca gcaggggcgc tacgacgagg cggcgaagct ctaccgccag tcgctgacga
     3601 tcgatccgaa tcgcgcggac gcgaagaaga acctcgagga ggcgattcgc cgcggacgga
     3661 accggccccc gcaggcgtcg tcgggagggc agggacaggg aggagagggg tcgtccgggt
     3721 cgggcacgtc gggacaatct ccgcccggat ctcagccgaa cacgccgccc ccgaccaggc
     3781 cgccgggctc tgcaccgcag ccgcccgggc agatgagcgg cacgccgagt ccctccgaag
     3841 cggagcactg gctgaacgcg ctcgaggagg agcggaagtc cgcacgccgc caggagcgcg
     3901 cgggctcgcc gcaggagacc aaggacaagg attggtgaca tggtgaatgg tggtcagcgg
     3961 agtgcggttc ttgcgccgac gttcctcctc gcgctcgcgg cggcgctggc gctccttccg
     4021 tcctccgcgc gggccgagca gctcaccgcc gaggccgagc tcgatcggca gcaggtcctc
     4081 gtcggggagc ccgcgaccct cgccatcacg atcacggccg aaggactcga cgtccccggc
     4141 atcaagctgc ccctgatcgc cggtctcgac gtgaccgcga cggagcaatc gcaagggttc
     4201 tcctggatga acggggtgat ctcacgcagc gtgacgagca cgttcgtgct ccgcgcgaac
     4261 gcccccggcg actacgtgat cccctccttc ctgattcact cgggcaagaa ggaagcgaag
     4321 acacaagagc tcgtgctcca cgtgcgcgca accatgccgc gctcgggcgg tggcttgggc
     4381 ggcgaaggag ggccggagct cttcgcgcgg ctcacgctcg accgcacgcg cgtctactgg
     4441 aacgaggggg tgacggcgcg cttcacgctc tactcgcgcg tgcgctatga aggcgcgccc
     4501 gcgtgggatc cccccgacgc gcccgggttc tggaccgagg tgctgggacc ggcgcgcacc
     4561 ggccggatcc gcgtgggcga cgtcgagtac gacgcgaccg agatgcgggt cgagtacttc
     4621 ccgacgcgca ccggaaagct ccggatcgga ccaggacgcg tgcacgcgca ggtcgtgcgg
     4681 caggtcccgg tccccgatcc ctggagcgcg ctcggcatgc cggagaccca ggtgcaggac
     4741 atcgaactca cgaccgagca gctcgcggtg gacgtcgtgc ccctgccgaa gggcgcgccg
     4801 tcgacgttcc gcggcgccgt gggcagctac gatctcgacg tgacggttga ccgcgccgtc
     4861 gtccgcgccc gcgagccggt gaacgtcgcc accgtgctca agggggaagg gaacctcgcg
     4921 gccgcgagcg atcccgacgt ctcgagcacg cttccctcgc gccgctacgc gggggccgcg
     4981 tcgacgggcc tcgatcgctc ggggctccgc ctgaaagggg agcgccgccg ggagacgagc
     5041 ttcgttcccg agcgacaggg atcgttcgcg atcctccccg ttcgcttctc atggttcgac
     5101 ccggagcagg ggcgctaccg gacccaggtg tccgacacga tcacggtgcg ggtcctgccg
     5161 ccgggctcgg gcagcgacac gctcggccct gcggccgtgc tcggacccgt ggcaccgcga
     5221 cgcgcgcatc ccggcgcggg cggctcgctc gcgctcgtgc cgccctggcc cgcggatgtg
     5281 gtcgcatggg gatcgctcct cgcctacctc ggcgtgctga ccgggctcgg cgtgcgcgac
     5341 cgttcgctcc gcgacccgcg gcggaagcgg gagcgcgagc tcgagacgat cgcgcgggag
     5401 ctcgcgcgtg cgggcaccgc ggcgccgacg ccggctcgta cggccgtgct cctacgccga
     5461 gcg
//
LOCUS       DASTKC010000195        18611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1106803, whole genome shotgun sequence.
ACCESSION   DASTKC010000195 DASTKC010000000
VERSION     DASTKC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18611
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106803"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /gene="ribA"
                     /locus_tag="VFR25_08035"
     CDS             <1..977
                     /gene="ribA"
                     /locus_tag="VFR25_08035"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET9941038.1"
                     /translation="GSSTQDRALTIRALANPKTRPEELARPGHVFPLRAADQGVLSRP
                     GHTEAALDLVRLAGLKPAAVLCEILSADGTMARGPELRRLAKKHGFPLITIRDLIRYR
                     YGKERLVHRVAVSRLPTRFGNFRIVVYESRIDGYHHVALVKGRAPARGRSTPAPLVRV
                     HSQCLTGDVFGSMRCDCGEQMASALERIDAEGSGVFLYMRQEGRGIGLANKLRAYELQ
                     DLGLDTVEANLKLGFAPDLRDYGVAAQILEDVGFTRVRLLTNNPRKIQALGEYGIDVE
                     SREPLEVRPNALNRRYLRTKRERLGHLLGHPHLELVAGKHRNGSRGRA"
     gene            1079..1525
                     /gene="ribH"
                     /locus_tag="VFR25_08040"
     CDS             1079..1525
                     /gene="ribH"
                     /locus_tag="VFR25_08040"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET9941039.1"
                     /translation="MAIVAARFNRAICDRLLDGALQALARRGVSAERVTVVRVPGAFE
                     VPFAALRLASSGRHDAVVALGAVIRGETPHFDYVAGESARGIARAAEATGVPVLFGIL
                     TTETPEQALERSGGAHGHRGADAAEAAIEMARLKRLLDQDAERRAR"
     gene            1522..1938
                     /gene="nusB"
                     /locus_tag="VFR25_08045"
     CDS             1522..1938
                     /gene="nusB"
                     /locus_tag="VFR25_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9941040.1"
                     /translation="MNRRREGREIAFRAVYQADVTGEPTDRCLGEIVEDVDPSEDVRE
                     FAASLVRTLDDHKAEIDATVERIARNWSLSRMAATDRSVIRVAAAELLYHFETPARVA
                     LDEAIEIAKKYGMETSGSFVNGILDRIAHEARPSVG"
     gene            1935..2660
                     /locus_tag="VFR25_08050"
     CDS             1935..2660
                     /locus_tag="VFR25_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9941041.1"
                     /translation="MTTAIISDIHGNLEALDAVLAELDRRRPDRVVCLGDIVGYGASP
                     NECLDRVRKRCGTVLLGNHDAAASGGPEAARFNIYARVAAEWTMKTLTRDNREYLQRL
                     PLTAQENSFFLVHASPACPRDWEYLLDRFDAEPQFHYFTEMICFIGHTHQPAIYLADQ
                     GGTKSLPLSEGKLDPNRRYIVNVGSVGQPRDHDPRACFVLYHESSTTIEYVRVPYDIE
                     GAQSKIRAAQLPEVLAARLATGE"
     gene            2663..3463
                     /locus_tag="VFR25_08055"
     CDS             2663..3463
                     /locus_tag="VFR25_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9941042.1"
                     /translation="MRASTEAHWSRFWRERAEIDEVYPTAGRVVEQMTAEGPVQGKIV
                     LEVGAGSGRDSVGLAEQGAVSVMLDYSMASLQVARDVAERSGVTPLLVRADALRMPFR
                     DGTFDAVFHQGLLEHFRDPMPLLRENVRALKKEGILLVDVPQRWHLYTAVKHALIAAG
                     RWFAGWETEFSIGELTGLMSRAGVRVVRRYGAWMVPGLAYRSLRLGLLKLRLARLPLD
                     PPPVPVITPLFAGIRGLVARTPLAFHTYFVIGAIGRKEAPEPPAARAG"
     gene            3460..4581
                     /locus_tag="VFR25_08060"
     CDS             3460..4581
                     /locus_tag="VFR25_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9941043.1"
                     /translation="MNLIAFNWRDPRHPEAGGAELHLFEILRRAVRDGDRAVWLAERY
                     PGAAPEDRIEGIEIHRAGTWYNAHLALGALYRRRFRRERFDLVVEDINKVPFFAPLYA
                     TAPVLAVVPHLFGTTAFNEASAPIAALVSAHESLIPIVYRKSRFLAISESTRDDLARR
                     GIARARIDVVRCGLTQGDYAVLVPPEERVDPEIVFLGRLRRYKGAQHAIRALPPILQS
                     VPGAHLTIVGDGPYRPELERLARELGVTERVRFLGAVPQAEKVAALNRAQVAVNPSPK
                     EGWGLTVIEANACGVPVVASQSPGLIESVRDAETGLLVPHGDHQALAAGIVKLLTDRD
                     LRLLLARAAIAWAHTFDWETCYRDSRRVMELAAREGARA"
     gene            4578..5630
                     /locus_tag="VFR25_08065"
     CDS             4578..5630
                     /locus_tag="VFR25_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9941044.1"
                     /translation="MSVGGVKGFYERYHVPLIVAKVALSLALFAYVVAKVSPGDIWAT
                     IRPADPGLLLLAGALLFFSSLVGGWLWARLLQAQGVNIPFRKAASYYFVGLFFNNFLP
                     SNIGGDIARISDASKHADNVSRVFTATLMDRLIGVLAIAFLALLASFASIDRFHLYAI
                     YGMIAIVFLVMLGLFISIFHRRVLEAFEWPFRVIGARGIERAIARLMDDLHGYKGQGS
                     ALVTAVAASMLVQVGRIVAHYLVGLALGVRVAMGYYFLFVPVLAALISLPISMNGLGV
                     REGAGVVLFQMSGMTKEQAFTVPFLTYLLSVLISLIGGLIFVTRPSRRAFQAMLEKKR
                     EREAETGDTQRRSIGP"
     gene            5627..8218
                     /locus_tag="VFR25_08070"
     CDS             5627..8218
                     /locus_tag="VFR25_08070"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9941045.1"
                     /translation="MTWKRGHALFAALSFFVPLLAYIRTITPTVPFWDSGEFIATSYI
                     LGLPHPPGNPVYTMLGRMMTFLPFETIAWRVNFMSSLASAIACYFTYLFTARAIRRTF
                     GNGEAGGDAEGSAPTTDLAWLASGVGGLVAALFLAFGSSFWDSAIEAEVYSLSSAIIA
                     LLVWLSFVWWDRQGEPGNDNLLVLIVYILGVSTGVHLGTILIAPSLLVLFWMVSPRVF
                     NSPKFLVPFLALGAFVVLLYLNGTMEMGLPDSVILGILGLGFLYALVQNKFFFRKNLV
                     AWWVLVGVIGISVQVFLLIRSQQMPAVNEGAPTTLGTWLEYLSRKQYGPANPFERRAE
                     LWYQIQHMYLRYVGQQWHLIERLGNLDLGSFWVRLVNAVPYLFFFAGAFWNYRRDKTT
                     FWFFLATHIIMGPGLIFYLNFTDHEVRERDYFFTNSYHFIAVWVGMGAAAALHALAGA
                     LAPAMAESAQQEAEPAVAAAPLPSGGSNRAVLGLGALTLTGLALLPMRTNWFEHDRSG
                     FYIAHDYAYNMLEPLEPGAVVFTNGDNDTFPLWYIQEVEKVRKDVRVVNLSLLNTPWY
                     IQQLRDQEPKVPISFTNAQLAVMGPYQDPNSGKIVWVKDLAVKDIVKTNQWKKPLYLA
                     VTVPEQMGLEKQLTLEGLVFRIEPKETQKQVDLAKTMHNLYSTFKYGGLLDKNRAYDT
                     TVYKDENAYRLVQNYTAAHVQVAYQLQLAQRNEDAVRVLEDAVKISPDFPGLLEYLGK
                     LYEDTGRADKAEQIYLEGVRRFPTQPEYHYHLGILYYQTGRVDPGIAELRRAAELNQQ
                     YFDWFSALFTALWQTGRRAEAVDVLRTWVRAHPEDAQGAGYLKVYEESLRVLGAGPPS
                     HAAPGRG"
     gene            8215..9159
                     /locus_tag="VFR25_08075"
     CDS             8215..9159
                     /locus_tag="VFR25_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9941046.1"
                     /translation="MTGLVLVTGAAGFIGSTLVEKLVASGADVIGVDAFTDNYPASQK
                     RQNIARLSGNRRFRLVEADLRTANIDPLLDGVDGVVHLAALPGVRASWGEPFRAYAEH
                     NVVATQRLLEAVRRHPVRRLVAASSSSIYGLPTRFPTPEDEAPRPVSPYGVTKLAGEA
                     LFHAYWASYRLPLVGLRYFTVYGPRQRPDMGIHAFFEAAKSGTPVKVYGTGDQTRDFT
                     FVDDAVAATISALTQPAVPGLTYNVGGGHRVSLKDLLARIELVTGRPLERRLVAPPPG
                     DPPDTSADVSRARRDLGYEPKTELTEGLRRQWEWQRKS"
     gene            9135..10112
                     /locus_tag="VFR25_08080"
     CDS             9135..10112
                     /locus_tag="VFR25_08080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9941047.1"
                     /translation="MGVAAEVVTAATRGGPGPELDLTVLIPVLNEAPNMEPLIRKLVQ
                     DLAPLKLSYEILVLDDGSTDGTFDEVRRLRPSTPGLRGIRFRRNFGKSAALSVGFQEA
                     RGRAVVTMDGDLQDDSAEIPRLLAALDEGYDLVSGWKQKRRDPIMKTIPSKLFNAVTS
                     AVSGVSLHDMNCGLKAYRQEVVRSVKLRGELHRFIPVLAYNAGFKVGEIRTVHHPRLH
                     GTSKFGMSRFLNGFLDLMAVQFMATSSTRPLHLFGRIGVTFALVGGLVTIATTWPWFL
                     GRGLHVRPALLFGLVLVIMGIQFLSLGFLGELIAGTRAEEPRYEIREYF"
     gene            10155..11288
                     /locus_tag="VFR25_08085"
     CDS             10155..11288
                     /locus_tag="VFR25_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941048.1"
                     /translation="MKVALLGPTYPHRGGIAHYTTLLARAFARDHETHLISFSRLYPS
                     LLFPGTTQLDRSGEAIQPPVPPEPLLDSMNPLTWVATGNRLRAIAPDLLVVTWWHPFF
                     GPSLGTAARRARRGARPKRIFLCHNVEPHEATPLDHALASYGLGAADGFLVHARAEAA
                     RLTPRAHGRPVRVHPHPSYEIFHRGAPPRDEARARLEVSGRVLLFFGYVRPYKGLGDL
                     LEALRQARPDSWDRLFVVGEFYEPRDRYARWLDDPALRAKITVVDRYVPNEEVGTYMA
                     AADLVVLPYRSATGSGIAQVAFGAGVPVIATRTGGLEDVVEDGVSGLLVPPESPGDLA
                     RAIERFADEDLGPKLRAGVERARTRFSWESLVDALVSLSGACA"
     gene            11285..12250
                     /locus_tag="VFR25_08090"
     CDS             11285..12250
                     /locus_tag="VFR25_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9941049.1"
                     /translation="MSAIPRETSPVWAVVLAFNEWDATRECLTSLRALDPAPDRTLLV
                     DNGSTDGTRERARAEFPEVEVLALGENRLFAGGVNAGLARALEGGAASVLLLNNDVVL
                     ERGALDHLATALAADEKRGAVSPKLYFWDPPDRIWFAGGVVTKGFGLIRHRGVHQKDD
                     AFRDGPRAVDYVSGAAVLLSRHALETVGLLDPDYVIYVEDVDWSARARKAGFTLWYEP
                     KARGWHHVSRTSGGGRLTPLKAYYRQRSGALYLTRHAPAWERPLAWMAYGLWMSYLLG
                     RAMAVPDVASSRALVQGYADFTTILFGGRAPDRRPEEFAPRTRPG"
     gene            complement(12174..13328)
                     /locus_tag="VFR25_08095"
     CDS             complement(12174..13328)
                     /locus_tag="VFR25_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9941050.1"
                     /translation="MARDIRVAIDVRPLALGEVTGVGLVVATLVEELERRGVRFVGVT
                     DRPVPPGRLPESVPVVTTGTAGGRIRWESGPLERALRAIDPSPDLFHATWNHGVPGGL
                     PFPSVLTVHDVIPWILPHLVPWPRPALLHRWLYRRALRASTRAAQLVMTDSEASRKDL
                     ARILPETAVKSEVVPISLPRWFRPLPPEAASETRAEWNQGRPYWLYLGGFDPRKGIGT
                     LLEAMALAFPDRSHAPDLILAGGLNALGLALRAEAGRLGVRARFPGYVDDRDLPSLFA
                     GATLFLYPSRYEGFGLPLLMAMASGVPTLASDAGSVPEVLGDAGFSFPVGNAASLATS
                     LARAAALTPEERAAIGERGRARAARFSPDAFAERILRAYDRALGRRTESS"
     gene            13463..14608
                     /locus_tag="VFR25_08100"
     CDS             13463..14608
                     /locus_tag="VFR25_08100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941051.1"
                     /translation="MRPSWDVVWLSDIPWAGLWQRPQQLATRFPEDARILFVEPWTLG
                     HDAAIRPAPVTERIQRVSFPFLPLHARSLRLRRVAYRLGGSGVATASLAALQRAWIGT
                     LGSFFRPHAKRLTLVENFMAYPTLRSWRPDRVVYDMIDAPLHFAPVPPRLVPAWEALL
                     REANRVVVTSGPLESLARHGGSARVARVGNGVEARRFAEAEPASLPGNAGAPVLGYVG
                     SLHAWFDVPLVGALARRIPEARVVLVGPAHPDTRNAIEREAATAPNLHWIGPKPYREI
                     PSIVAAFRVGLIPFRRTPLTEAVNPVKLYEYAAAGVPCVTTRFSDEVDSWRRAAMVAD
                     TEDGFVLAVRGELEREPDRAQLRAFASEHDWDAIAARFTQVVLEDAA"
     gene            14605..17064
                     /locus_tag="VFR25_08105"
     CDS             14605..17064
                     /locus_tag="VFR25_08105"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET9941052.1"
                     /translation="MSPAKKKIGVRPVAARGAGRGGSIRPADSSDLKALWAYALLAGL
                     VLLFFYPITSGKVFVSPDSVAPAGFVKVADQALQTRHVYPLWNPYTFLGMPSFGSLAF
                     VPYAYPPDPIFGFLNVHLGFPDLTWLLAHYLLLGFSMYAFLRAFGTSRGPSILGALTL
                     ALTPNLVAVGAFGHGSQIMTAAYLPLLLLLYDRFARRGSWLALSGFAVAASFQLLRGH
                     VQIVFYSWLALGLYALFLAVDAFRSGRRGEGVRAIGGLAGGLALAFGLSAFLYLPVHE
                     YSKLSTRGGGEGGGAGLAYATSWSFHPREILTFILPGFFGFGGATYWGSMPFTDYPNY
                     MGIVALVLAIYGAASTRGRTRTYLIVLAVVALLVSFGKHFQPLYALLYYHLPFFNKFR
                     VPVMILVLVQFAVAALAALGLDRALTPDRQAQGKDPGSPWMRGALLSLAGGFGLLVAL
                     NALGAMYRTAAMSSRQGYDEARARAALDLASTDALKAGLLLAAALFVIGLARRGRLSR
                     VGASLLVILIAVVDLWPVDRKIIDPQVGTAVEYDANFKETPEIAFLRADTTQYRVFPL
                     QWNDSRLAAFGIASVLGYHPAKPYLYQAFVDTVGIQSFGTLEMLNVKYVLADGYFPPE
                     AQDKAALRHDGEVKVYEILHTMPRASVIHAVRPVRDDGVALATMRTGGFDPHHEILWS
                     LPGAPPDVAAPSTPDSVRTLRYDFNDIEYFVSTTAPGVFLTIEEYDPDWKAFVDGKPV
                     TIERVNYFMRGVPLEPGVHHVRYQYEPKALAEGIRVSIASAVVAALIALWGVKQLLDE
                     RRRRRTAAAPAPPATPEGAAG"
     gene            17061..17774
                     /locus_tag="VFR25_08110"
     CDS             17061..17774
                     /locus_tag="VFR25_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET9941053.1"
                     /translation="MKALIIVPTYNERENLEELLRRIFDQKLPTEILVVDDNSPDGTG
                     ALADEMAARDPRVHVMHRPGKMGLGSAYVAGFKWALARDYDAVFEMDADFSHNPDSLG
                     DFLRELGHADLVLGSRYLHGVTVVNWPLSRLILSYSANLYSRIITGMPVKDLTGGFKC
                     FRRQVLEAIDLDRVRSDGYAFQIEINFKAWRKGFRIQEIPILFVDRQAGVSKMSRRIV
                     REAAWMVWRLRLLDLLGAL"
     gene            17831..>18611
                     /locus_tag="VFR25_08115"
     CDS             17831..>18611
                     /locus_tag="VFR25_08115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9941054.1"
                     /translation="MERLFQSLREARPAPLREIVVVNNSGEPLDDLARASGYPTRVLT
                     PGSNLGYARGVNEGIRAASEENVLLLNPDVRVLPGSVEALERAAAEHPRAGIVAPKLL
                     ESDGSLQLSARRFYTWRTLLLRRMPLPGVAARSREVRDHLMEDWDHADTRAVDWVLGA
                     AMYVRRGAMRDVGLMDERYFLYFEDVDWCQRMWRHGYEVVYCADARMVHEYERASAQM
                     RPRSVRAHAAGLLRFTEKWSAILYAMSQHRRRMIQVITLAMD"
BASE COUNT         2729 a         6531 c         6297 g         3054 t
ORIGIN      
        1 cgggatcgtc gacgcaggat cgcgcgctca cgatccgggc cctggcgaat cccaagacgc
       61 gcccggagga gctcgcgcgg ccggggcacg tgttcccgct gcgagcggcc gaccaggggg
      121 tcctgagccg cccggggcac acggaggcgg cgctcgatct cgtgcgcctc gcgggcctga
      181 agccggccgc ggtcctctgc gagatcctct ccgcggacgg gaccatggcg cgcggccccg
      241 agcttcgacg gctcgcgaag aaacacggct ttccgctcat cacgatccgg gatctcatcc
      301 gctatcgcta cgggaaggag cgcctcgtcc atcgcgtcgc ggtctcgcgg cttccaaccc
      361 gcttcggcaa cttccgcatc gtggtctacg agagccggat cgacggctac caccacgtgg
      421 ccctggtgaa ggggcgcgcg cccgcgcgcg gccgctccac gccggcgccg ctcgtgcggg
      481 ttcattcgca gtgcctcacg ggggacgtgt tcgggtccat gcgctgcgac tgcggcgagc
      541 agatggcctc ggcgctcgaa aggatcgacg ccgagggatc gggcgtgttc ctctacatga
      601 ggcaggaagg ccggggcatc gggctcgcga acaagctccg cgcgtacgag ctgcaagact
      661 tggggctcga caccgtcgag gcgaatctga agcttggatt cgcgcccgat ctcagggact
      721 acggcgtggc ggcgcaaatc ttggaggatg tcgggttcac ccgcgtgcgg ctcctgacca
      781 acaatccgcg aaagatccag gcgctcggcg agtacgggat cgatgtcgag tcgcgcgagc
      841 cgctcgaggt gcgaccgaac gccctgaacc ggcgctacct ccgcaccaag cgcgagcggc
      901 tgggacacct tctcggtcat ccgcatctcg agctcgtggc cggcaagcac cggaacgggt
      961 cgcgggggcg cgcgtgacct cgggagcgaa ggacgcggga tacgcgccac cgctcgccgc
     1021 ggccggctcg caggggcacc gggtcctcga gggatcgctc gacggcgccg ggctctcgat
     1081 cgcgatcgtg gccgcgcgct tcaaccgggc gatctgcgac cggctcctcg atggggcgct
     1141 tcaagcgctc gcgcggcgcg gcgtgtccgc cgagcgcgtg accgtggtgc gcgtgccggg
     1201 agcgttcgag gtcccgttcg cggcgctcag gctcgcgtcc tcgggacgcc acgacgccgt
     1261 ggtcgcgctc ggcgcggtga ttcgcggcga gacgccccac ttcgactacg tggccggcga
     1321 atcggcgcgc gggattgcgc gggcggctga agcgacgggc gtgcccgtcc tgttcgggat
     1381 cctgacgacc gagactccgg agcaagcgct ggagcgctcc ggcggagcgc atggacatcg
     1441 cggcgccgac gcggccgaag cggcgatcga gatggccagg ctcaagcgcc tgctcgacca
     1501 ggatgcggag cgccgcgcgc gatgaatcgt cgccgggaag gacgcgagat cgcgtttcgc
     1561 gccgtctatc aggccgacgt cacgggtgag ccgacggacc gctgcctggg cgagatcgtg
     1621 gaagacgtcg acccgagcga ggacgtgcgc gaattcgccg cgagcctcgt ccgaaccctc
     1681 gacgatcaca aggccgagat cgacgcgacc gtcgagcgca tcgcccggaa ctggtcgctc
     1741 agccggatgg ccgcgaccga ccggtcggtg atccgcgtcg ccgcggcgga gctcctctac
     1801 cacttcgaaa cgccggcgcg ggtcgccctg gacgaggcga tcgagatcgc gaagaagtac
     1861 ggaatggaga cctcggggtc cttcgtgaac ggaatccttg accggatcgc ccacgaagcg
     1921 cgaccctccg tcggatgacc accgccatca tctcggacat ccacggtaat ctcgaagcgc
     1981 tcgacgcggt gctggccgag ctcgaccggc gccgtccgga tcgcgtcgtc tgcctcggcg
     2041 acatcgtcgg ttacggagcg agcccgaacg agtgtctcga ccgcgtgcgg aagcgctgcg
     2101 gcaccgtgct cctcggcaat cacgacgccg ccgcgagcgg cggtccggag gcggcgcggt
     2161 tcaacatcta cgcgcgggtc gcggccgagt ggacgatgaa gaccctgacg cgcgacaacc
     2221 gggaatacct ccagcgcctt ccgctcaccg cgcaggagaa ctccttcttc ctcgtgcacg
     2281 cgtcccccgc ctgcccgcgg gactgggagt acctgctcga ccggttcgac gcggagccgc
     2341 agttccacta cttcacggaa atgatctgct tcatcgggca tacccaccag ccggccatct
     2401 acctcgccga ccagggcggc accaagtcgc tgcctctctc ggaagggaag ctcgatccga
     2461 atcgccgcta catcgtgaac gtggggagcg tggggcagcc gcgcgaccac gatccccgcg
     2521 cctgcttcgt tctctatcac gagtcctcca ccaccatcga atacgtgcgc gtcccctacg
     2581 acatcgaggg ggcgcaatcg aagatccggg ccgcccagct tccggaagtc ctcgccgcgc
     2641 gcctcgccac cggggagtaa ccgtgcgggc gtcgaccgaa gcgcactgga gccgcttctg
     2701 gcgggagcgg gccgagatcg acgaggtcta tccgaccgcc ggtcgcgtcg tcgagcagat
     2761 gacggccgag ggtcccgtcc aggggaagat cgtcctcgag gtgggagccg gatcggggcg
     2821 ggacagcgtg ggactggccg agcagggggc ggtctccgtg atgctcgact actcgatggc
     2881 gtcgctccag gtggcgcgcg acgtggcgga gcggtcgggc gtgaccccgc tcctggtccg
     2941 tgccgacgcg cttaggatgc cgtttcggga cggcacgttc gacgccgtct ttcaccaagg
     3001 gctgctggag cactttcgcg atccgatgcc gctcctccgg gagaacgtgc gcgcgctcaa
     3061 gaaggaggga atcctcctgg tcgacgtgcc gcagcgctgg catctctaca ccgcggtcaa
     3121 gcacgcgctc atcgccgccg gacgatggtt cgcgggatgg gagaccgagt tctcgatcgg
     3181 cgagctgacc gggctcatgt cgcgcgcggg cgttcgcgtg gtccggcgct acggcgcctg
     3241 gatggtgccc ggcctcgcgt atcgctcgct caggctcggg ctcttgaagc tcaggctcgc
     3301 ccgcctgccg ctggatccgc cgccggttcc cgtgatcaca cccctcttcg ccggtatccg
     3361 cgggctcgtc gcgcggacgc cgctcgcctt ccatacctac ttcgtgatcg gcgcgatcgg
     3421 acgcaaggag gcgccggaac ctccggctgc ccgagccggt tgaacctcat cgcattcaac
     3481 tggcgcgatc cgcgccaccc ggaagccggc ggagccgagc tccatctctt cgagatcctc
     3541 cgccgcgccg tccgcgacgg ggaccgggcg gtctggctgg cggagcgcta tcccggtgcg
     3601 gcgccggaag atcggatcga gggcatcgag attcaccgtg cgggtacgtg gtacaatgcc
     3661 caccttgccc tcggggctct ctatcgtcgt cggttccgga gggagcggtt tgacctcgtc
     3721 gtggaagaca tcaacaaggt tccgttcttc gccccgctct acgcgacggc gccggtcctg
     3781 gccgtcgtcc ctcatctctt cggcaccacc gccttcaacg aagcgtccgc accgatcgcc
     3841 gcgctggtct ccgcacacga gagtttgatc ccgatcgtgt accggaagag ccgcttcctc
     3901 gcgatctccg agagcacgcg ggacgacctg gcgcgtcgcg gaatcgcgcg cgcgcggatc
     3961 gacgtggtgc gctgcggcct gacccagggg gactacgccg tcctggttcc gcccgaagag
     4021 cgagtcgacc ccgagatcgt attcctcggc cggctccggc ggtacaaggg ggcgcagcac
     4081 gcgatccgcg cgcttcctcc gattctccag tccgtgccgg gtgcgcatct cacgatcgtc
     4141 ggcgacgggc cgtaccgccc ggagctcgag cgcctcgcgc gcgagctcgg cgtgacggaa
     4201 cgggtccgct tcctcggagc cgtacctcaa gcggagaagg tggccgcgct gaatcgcgcg
     4261 caagtcgcgg tcaacccctc gcccaaggaa ggctgggggc tcaccgtgat cgaggcgaat
     4321 gcgtgcggcg tccccgtggt cgcgagccag agcccgggct tgatcgagtc ggtgcgggac
     4381 gcagagacgg ggctcctcgt cccgcacggc gaccaccagg cgctcgcggc gggcatcgtg
     4441 aagctcctca ccgatcgcga tctcaggctc ctcttggctc gcgccgcgat cgcgtgggcg
     4501 cacacgttcg attgggagac gtgctatcgg gacagccgcc gggtgatgga gctggcggct
     4561 cgggaaggcg cgcgggcgtg agcgtcggcg gcgtgaaggg gttctacgag cgctatcacg
     4621 taccgctgat cgtggcgaag gtagccctga gcctcgcgct cttcgcctac gtcgtcgcga
     4681 aggtctcgcc cggggacatc tgggccacga tccgtcccgc cgatcccggg cttctcctcc
     4741 tcgcgggcgc gctcctcttc ttctcgagcc tcgtgggcgg atggctctgg gcgcggctcc
     4801 tccaggcgca gggcgtgaac atcccgttcc ggaaagccgc gtcgtattac ttcgtcggcc
     4861 tcttcttcaa caacttcctc ccgtccaaca tcggcggcga catcgcgcgg atctccgacg
     4921 cctcgaagca cgccgacaac gtgagccggg tgttcacggc cacgctcatg gaccgcttga
     4981 tcggcgtcct cgcgatcgcg ttcctggcgc tcctcgcgtc gttcgcgtcc atcgaccggt
     5041 tccacctgta cgccatctac gggatgatcg cgatcgtgtt cctggtgatg ctcggcctct
     5101 tcatcagcat cttccaccgg cgggtgctgg aggcgttcga gtggcccttc cgcgtgatcg
     5161 gcgcgcgggg gatcgagcgc gcgatcgcgc gcctgatgga cgatctgcac ggctacaagg
     5221 ggcaggggag cgcgctcgtc acggccgtcg ccgcgtcgat gctggtccag gtcggacgga
     5281 tcgtggcgca ctacctcgtg ggcctcgccc tcggcgtgcg ggtggccatg ggctactact
     5341 ttctcttcgt gcccgtgctc gcggctctga tctcgctccc gatctcgatg aacggcctcg
     5401 gcgtccgcga aggagcgggg gttgtgctgt tccagatgtc gggcatgacc aaggagcagg
     5461 cgttcacggt accgtttctc acctacctcc tctccgtcct catcagcttg atcggagggc
     5521 tcatcttcgt gacccgaccc tcgcgtcgcg ccttccaggc gatgctcgag aagaagcgcg
     5581 agcgcgaggc ggagaccgga gacacccaaa ggagaagcat cggcccatga cctggaagcg
     5641 cggccacgcc ctgttcgcgg ccctctcctt cttcgtcccg ctcctggcgt acatccgcac
     5701 catcacgccg acggtgccgt tctgggactc cggggagttc atcgcgacgt cctacatcct
     5761 cggtctcccg cacccgccgg ggaacccggt ctacaccatg ctcgggcgga tgatgacgtt
     5821 cctgccgttc gagaccatcg cgtggcgggt gaacttcatg agctcgcttg cgtcggccat
     5881 cgcctgctac ttcacctacc tcttcaccgc gcgcgccatc cgccgcacgt tcgggaacgg
     5941 cgaggcgggg ggcgacgccg agggctccgc tccgacgacg gatctcgcct ggctcgcgag
     6001 cggggtgggc gggctcgtcg ccgcgctctt cctcgccttc ggctcgtcct tctgggacag
     6061 cgcgatcgag gcggaggtct actcgctctc gtcggcgatc atcgcgctcc tcgtctggct
     6121 cagcttcgtc tggtgggacc ggcaggggga gccggggaac gacaacctcc tcgtcctcat
     6181 cgtgtacatc ctcggcgtct cgaccggcgt ccatctcggc acgatcctga tcgccccgtc
     6241 cctgctcgtc ctcttctgga tggtgagccc gcgcgtcttc aacagtccga agttcctcgt
     6301 gccgttcctc gcgctcggcg ccttcgtcgt gctgctctac ttgaacggca ccatggagat
     6361 gggtctgccg gactcggtca tcctggggat cctcgggctg ggattcctct acgcgctcgt
     6421 gcagaacaag ttcttcttcc ggaagaacct cgtcgcttgg tgggtcctcg tcggggtgat
     6481 cgggatctcg gtgcaggtct tcctcctcat ccggagccag cagatgccgg ccgtgaacga
     6541 gggggcgccc accacgctcg ggacctggct cgagtatctc agccgcaagc agtacgggcc
     6601 ggccaatccc ttcgagcgcc gggcggagct ctggtaccag atccagcaca tgtacctgcg
     6661 ctacgtcggg cagcagtggc acctgatcga gcggctgggg aacctcgacc tggggtcctt
     6721 ctgggtccgg ctcgtgaacg cggtcccgta cctcttcttc ttcgcgggcg ccttctggaa
     6781 ctatcgccgc gacaagacga cgttctggtt cttcctcgcg acccacatca tcatggggcc
     6841 cgggctcatc ttctatctca acttcaccga ccacgaagtc cgcgagcgcg actacttctt
     6901 cacgaactcg tatcacttca tcgccgtgtg ggtcgggatg ggcgcggccg cggcgctcca
     6961 cgccctggcg ggtgcgctcg cgccggccat ggccgagtcg gcacagcagg aggccgagcc
     7021 ggcggttgcg gccgctccgc ttccgtccgg gggttccaac cgcgcggtcc tcgggctggg
     7081 cgcgctcacg ctcaccggcc tggcgctctt gcccatgcgg accaactggt tcgagcacga
     7141 ccgctccggc ttctacatcg cccacgacta cgcctacaac atgctggagc cgctcgagcc
     7201 cggcgcggtc gtgttcacga acggggacaa cgacacgttc ccactctggt acatccagga
     7261 ggtggagaaa gtccgtaagg acgttcgggt cgtcaacctg agccttctca acactccctg
     7321 gtacatccag cagctccgtg accaggagcc gaaggtcccg ataagcttca cgaacgcaca
     7381 gcttgccgtg atgggtccgt accaggaccc gaattcaggg aagatcgtct gggtgaagga
     7441 cctggcggtg aaggacatcg tgaagacgaa ccagtggaag aagccgctct atctggcggt
     7501 caccgtgccg gagcagatgg ggctcgagaa gcagctcacg ctcgaggggc tcgtcttccg
     7561 catcgagccg aaggaaacgc agaagcaggt cgatctcgcg aagacgatgc acaacctcta
     7621 ctcgacgttc aagtacggcg ggctgctcga caagaaccgc gcctacgaca cgaccgtgta
     7681 caaggacgag aacgcctacc gcctcgtcca gaactacacg gcggcccacg tccaggtggc
     7741 gtatcagctg cagctcgcgc agcggaacga ggatgcggtc agggtcctcg aggacgcggt
     7801 caagatctcg cccgacttcc cgggcctgct cgagtacctg ggcaagctgt acgaggacac
     7861 ggggcgcgct gacaaggcgg agcagatcta tctcgaggga gtccggcgct tcccgacgca
     7921 gcccgaatac cactatcacc tcgggatcct ctattaccag accggccgcg tggatccggg
     7981 catcgccgag ctcaggcgcg ccgcggagct gaaccagcag tacttcgact ggttcagcgc
     8041 gctcttcacc gcgctctggc agacgggccg ccgcgccgag gcggtcgacg tgctccgcac
     8101 atgggtgcgg gcgcaccccg aggacgccca gggagccggc tacttgaagg tctacgagga
     8161 gtcgctccgc gtgctcggtg ccggtccgcc gtctcacgcg gctccggggc gcggatgaca
     8221 gggctcgtgc tggtcacggg agccgccggg ttcatcggct ccacgctcgt cgagaagctg
     8281 gtcgcctcgg gagcggacgt gatcggcgtg gacgccttca ccgacaacta tcccgcgtcg
     8341 cagaagcgcc agaacatcgc ccgcctgagc gggaatcggc gcttccgcct ggtcgaggcc
     8401 gatctccgga ccgcgaacat cgacccgctg ctcgacggcg tggacggggt cgtccatctg
     8461 gcggcgctcc cgggcgtgcg cgcgagctgg ggggagccgt ttcgcgcgta tgccgagcac
     8521 aacgtcgtcg cgacgcagcg gctgcttgag gcggtccgcc gccatcccgt gcgccgcctg
     8581 gtcgcggcgt cgagctcgtc gatttacggg ctgccgacgc gctttccgac gccggaggac
     8641 gaggcgccgc gccccgtgtc gccgtacggc gtgacgaagc tcgcggggga agcgctcttc
     8701 cacgcgtact gggcgagcta ccggctgccg ctcgtggggc tccgctactt caccgtctac
     8761 ggcccgaggc agcgccccga catgggtatc catgctttct tcgaagcggc caagagcggc
     8821 acgcccgtga aggtctacgg caccggggat cagacccgcg acttcacctt tgtcgacgac
     8881 gcggtcgccg ctaccatctc cgcgctcacc cagcccgcgg tgccggggct gacgtacaat
     8941 gtcggcggcg gtcaccgcgt gtcgctcaag gacttgctcg cgcgcatcga gctcgtcacg
     9001 ggacgtccgc tcgagcggag actcgtggcg ccgccgcccg gcgatccgcc cgacacgtcg
     9061 gccgacgtgt cgcgggcgcg acgcgatctg ggctatgagc cgaaaacgga gctgacggag
     9121 ggactgaggc gccaatggga gtggcagcgg aagtcgtgac cgcggcaacg cggggcgggc
     9181 ccggaccgga gctcgatctc accgtactca tccccgttct gaacgaggcg ccgaacatgg
     9241 agcctctcat ccggaagctg gtccaggatc tcgcgccgct taagctctcc tacgagatcc
     9301 tggtcctcga cgacggatcg acggatggca cgttcgacga ggtgcgccgt ctgcggccgt
     9361 cgacgcctgg ccttcgaggc attcgctttc gtaggaattt cgggaagtcg gcggcgctct
     9421 cggtgggctt ccaggaagcc cgcggccgcg cggtcgtgac catggacggg gatctccagg
     9481 acgactcggc ggagatcccg cgcctcctgg ccgcgctcga cgaaggctac gatctcgtct
     9541 cggggtggaa gcagaagcgc cgcgacccca tcatgaagac gatcccctcg aagctcttca
     9601 acgcggtgac gtcggcggtg agcggcgttt cgctccacga catgaactgc ggactcaagg
     9661 cctaccggca agaagtggtg cgctcggtca agctccgggg cgagctccac cggttcatcc
     9721 cggtgctcgc gtacaacgcc ggcttcaagg tcggcgagat ccgcaccgtg caccacccgc
     9781 gcctccacgg cacgtccaag ttcgggatgt cgcgcttcct gaacggattc ctcgacctga
     9841 tggccgtcca gttcatggcg acgagcagca cgcgtccgct ccatctcttc ggacggatcg
     9901 gcgtcacctt cgcgctcgtc ggcggcctcg tgacgatcgc gacgacgtgg ccctggttcc
     9961 tcgggcgggg actccacgtg cgtcccgcac tcctcttcgg cctcgtgctc gtcatcatgg
    10021 gcatccagtt cctctcgctc ggcttcctcg gcgagctgat cgccgggacg agggcggaag
    10081 agccgcggta cgagatccgg gagtactttt agcgatgaag cgacacgtgc gaagcacgtg
    10141 tcggaacgca tgagatgaag gtcgcccttc tcggcccgac ctaccctcat cgcggcggca
    10201 tcgcgcacta cacaacactc ctcgcgcgcg cctttgcgcg cgaccacgaa acccacctca
    10261 tctccttcag ccgcctctat ccctcgctcc tcttcccagg cacgacgcaa ttggatcgaa
    10321 gcggcgaagc catccaacct cccgtgccgc ccgagccgct cctcgacagc atgaatccac
    10381 tgacctgggt cgcaacaggc aaccgcctgc gcgcgatcgc gccggatctc ctggtcgtca
    10441 cctggtggca tccgttcttc ggcccgtcgc tcgggaccgc ggcgcggcgc gcacggcgtg
    10501 gcgctcgccc caagcgaatc ttcctctgcc acaacgtgga gccgcacgag gcgacccctc
    10561 tggaccacgc gctcgcctcc tacgggctcg gcgcggccga cggcttcctc gtccacgcgc
    10621 gcgccgaggc ggcgcgcctc acgcctcgcg cgcacggacg tccggtgcgc gtccatccgc
    10681 atccgtcgta cgaaatcttc catcgcggcg ccccgccgcg cgacgaagcg cgcgcgcggc
    10741 tggaggtcag cggacgcgtg ctcctcttct tcggatacgt ccgcccgtac aaggggctcg
    10801 gcgatctcct cgaggcgctc cgccaggcgc gccccgactc gtgggaccgg ctcttcgtgg
    10861 tgggggagtt ctacgagccg cgcgaccgat acgcccggtg gctcgacgat cccgcgctgc
    10921 gcgcgaagat caccgtcgtg gatcgatatg tgccgaacga ggaagtcggg acgtacatgg
    10981 ccgcggccga cctcgtggtg ctcccgtatc gctccgccac cggctcgggc atcgcccaag
    11041 tcgcgttcgg cgccggcgtc cctgtgatcg cgaccaggac gggcgggctc gaggacgtgg
    11101 tcgaggacgg cgtgtccggt ctcctcgttc cccccgagtc gcccggtgat ctcgctcgcg
    11161 cgatcgagcg cttcgcggac gaggatctcg gaccgaagct ccgggcgggc gtcgagcgcg
    11221 cgcgcacccg gttcagctgg gagtcgctcg tggatgcgct cgtgagcttg agcggagcgt
    11281 gcgcgtgagc gcgatcccgc gcgagacatc gcccgtctgg gcggtggtcc tcgccttcaa
    11341 cgagtgggac gcgacgcgcg agtgcctgac ctcgctccgc gcgctcgacc ccgctcccga
    11401 ccggacgctc ctcgtcgaca acgggtcgac ggacgggacc agggagcggg cgcgcgccga
    11461 gttccccgaa gtcgaggtgc tggcgctcgg tgagaaccgg ctcttcgccg gcggggtgaa
    11521 cgctgggctc gcgcgggcgc tcgagggggg cgcggcttcg gtgctcctcc tcaacaacga
    11581 cgtggtgctc gagcgggggg ctctcgacca cctcgcgacc gcgctcgcgg ccgacgagaa
    11641 gcgcggcgcg gtcagcccga agctctactt ctgggatccg cccgaccgga tctggttcgc
    11701 ggggggcgtc gtgacgaagg gattcggtct gatccgccac cgcggcgtgc accagaagga
    11761 cgacgcgttc cgggacggcc cgcgagccgt cgactacgtc tcgggggccg cggtgctcct
    11821 ctcgcgccat gcgctcgaaa cggtggggct cctggatccc gactacgtga tctacgtcga
    11881 ggacgtcgac tggtcggcgc gcgcgcggaa ggcgggattc acgctctggt acgagcccaa
    11941 ggcgcggggc tggcaccacg tgtcgcggac gagcggcggc ggacgactca cgccgctcaa
    12001 ggcctactac cgccagcgga gcggagcgct ctacctcacg cgccacgcgc ccgcgtggga
    12061 gcggccgctg gcgtggatgg cctacggcct ctggatgtcc tatctcctcg gccgcgccat
    12121 ggccgtgccg gacgtcgcgt cctcgcgcgc gctcgtccag ggatacgccg acttcacgac
    12181 gattctgttc ggcggccgag cgcccgatcg taggcccgaa gaattcgctc cgcgaacgcg
    12241 tccgggctga acctggcggc gcgggcgcgg ccccgctcgc cgatcgccgc gcgctcctcc
    12301 ggcgtgagcg cggccgcacg ggcgagtgac gtcgcgagcg aggccgcgtt tcccacgggg
    12361 aacgagaatc ccgcgtctcc cagcacctcg ggcaccgatc ccgcgtcgct cgccagcgtc
    12421 ggaactcccg aggccatggc catgaggagc ggaagcccga agccctcgta gcgactggga
    12481 tagaggaaga gcgtggcccc ggcgaagagc gacggcagat cccgatcgtc cacgtagccc
    12541 gggaagcgtg cgcgcacgcc gagccgcccg gcctcggcgc ggagcgcgag acccagggca
    12601 ttgagtccac cggccaggat gagatccggc gcgtgcgacc gatcggggaa ggcgagcgcc
    12661 atggcctcga gcagggtccc gattcccttg cgcggatcga agccgcccag gtagagccag
    12721 taggggcgcc cttgattcca ctccgcgcga gtctcggacg ccgcctcggg cgggagcggt
    12781 cggaaccagc gtgggagcga gatcggcacg acctccgact tcaccgccgt ctcgggaaga
    12841 atgcgcgcca ggtccttccg gctcgcttca ctgtcggtca tcacgagttg ggcggcgcga
    12901 gtcgacgctc ggagcgcgcg gcgatagagc catcggtgaa gcagggcagg gcgcggccat
    12961 gggacgagat ggggaaggat ccaggggatc acgtcgtgca cggtgagaac cgacgggaag
    13021 ggaagtccgc ccggcacgcc atgattccag gtcgcgtgga agagatcggg ggaagggtcg
    13081 atcgcgcgga gcgcccgctc gagcggaccc gactcccacc gtatgcgtcc tccggcggtg
    13141 cctgtcgtga cgacgggaac ggattcgggg agccggcccg gcggaacggg acgatcggtg
    13201 acgccgacga agcggacgcc ccgcctctcg agctcctcca cgagcgtggc cacgacgagg
    13261 ccgaccccgg tcacttcgcc gagggccagg ggcctcacgt cgatcgccac acgaatgtcg
    13321 cgcgccacgg cggcacgcta cgggagcggg ctcgtccggg tcaatgcccc gcgaggccgg
    13381 gcaaggaagc gcgcatcctc cggcttgact tagccttgcg cccgcacgta ggctgttccg
    13441 gtgcgcgacc acggaggctc acgtgcgtcc ctcgtgggac gtcgtctggc tgagtgacat
    13501 tccgtgggcc ggcctctggc agcggcctca gcagctcgcc acgcgtttcc ccgaggacgc
    13561 gcgcatcctc ttcgtcgagc cctggacgct cggacatgat gccgcgatcc ggccggcgcc
    13621 ggtcacggag cgcatccagc gcgtctcgtt cccatttctt ccgctccacg ctcgctcgct
    13681 tcgactacgg cgggtcgcgt accgactggg cggctcgggc gtcgcgaccg cgtcgctcgc
    13741 cgcgctccag cgagcatgga tcggaacgct ggggagcttc ttcaggcccc atgcgaagcg
    13801 gctgaccctg gtggagaact tcatggccta tccgacgctc cgctcctggc gtcccgaccg
    13861 cgtcgtctac gacatgatcg atgcgccgct ccatttcgcc cccgtgcccc cgcgcctcgt
    13921 gccggcgtgg gaggcgctcc tccgggaggc gaatcgcgtc gtcgtgacgt cggggccgct
    13981 cgagtcgctc gcccgtcacg gcggctccgc gcgcgtcgca cgcgtcggaa acggcgtcga
    14041 agcgcgccgc ttcgcggaag ccgagccggc gtcgctgccg gggaacgcgg gcgctcccgt
    14101 cctgggttac gtcggctccc tccacgcgtg gttcgacgtt cccctggtcg gcgcgctcgc
    14161 gcgccggatt ccggaagccc gcgtcgtcct cgtcggtccc gcgcatccgg acacgaggaa
    14221 cgcgatcgag cgcgaggcgg cgacggcccc caatctccac tggatcggcc ccaagccgta
    14281 ccgtgagatc ccatccatcg tcgccgcgtt tcgcgtgggc ttgattccat tccgacgcac
    14341 accgctcacc gaggcggtga atccggtgaa gctctacgag tacgcggctg cgggcgtgcc
    14401 gtgcgtgacc acacggttca gcgacgaggt cgactcgtgg cgccgcgcgg cgatggtcgc
    14461 ggacacggag gacggcttcg tccttgctgt gcggggcgag ctcgagcgcg agcccgaccg
    14521 ggcgcagctt cgcgcgttcg cctcggagca cgactgggac gcgatcgcgg cgcggttcac
    14581 gcaggtcgtg ctcgaggacg cggcgtgagc ccggcgaaga agaagatcgg agtgcgcccc
    14641 gtcgcggcgc gaggcgccgg gcgcggcgga tcgatccgtc ccgccgacag ctccgacctc
    14701 aaggcgctct gggcgtacgc gctcctcgcg gggctcgtgc tcctcttctt ctatccgatc
    14761 acctccggca aggttttcgt ctcgcccgac tcggtcgctc ccgcgggctt cgtgaaagtc
    14821 gccgaccagg cgctccagac gcgccacgtc tacccgctct ggaacccgta cacgttcctc
    14881 ggcatgccga gcttcgggag cctcgccttc gtcccctacg cgtatccccc cgacccgatc
    14941 ttcggcttcc tgaacgtgca cctgggattt cccgatctca cgtggctgct cgcccactat
    15001 ctcctgctcg gcttctcgat gtacgccttc ctgcgagcgt tcgggacgtc acgcggaccg
    15061 tcgatccttg gagcgctgac gctcgccttg acccccaatc tcgtcgctgt cggggcgttc
    15121 gggcacggaa gccagatcat gacggcggca tacctgccgc tccttttgct cctctacgat
    15181 cggttcgcgc ggcgtggatc gtggctcgcg ctctccggat tcgcggtcgc ggcctccttc
    15241 cagctcctgc gcgggcacgt gcagatcgtc ttctactcgt ggctcgcgct cgggctctat
    15301 gcgctcttcc tcgcggtcga cgcgttccgc tccggacgcc gcggcgaggg ggtgcgcgcg
    15361 atcgggggac tcgcgggcgg gctcgcgctc gccttcgggc tgagcgcctt cctctacctg
    15421 ccggtgcacg agtactcgaa gctctcgaca cgcggcggcg gcgagggagg aggcgcggga
    15481 ctcgcgtatg cgacctcctg gtcgttccat ccgcgcgaga tcctgacgtt catcctgccc
    15541 ggcttcttcg gcttcggcgg cgcgacctac tgggggagca tgccgttcac cgactatccc
    15601 aactacatgg ggatcgtggc gctcgtgctc gccatctacg gcgcggcgag cacgcggggc
    15661 cggacgcgca cgtacctgat cgtgctcgcg gtggtcgcgc tcctcgtctc cttcggcaag
    15721 cacttccagc cgctctacgc gctcctctac taccatctcc cgttcttcaa caagttccgc
    15781 gtgccggtga tgatcctcgt cctcgttcag ttcgccgtgg ccgcgctcgc cgcgctcgga
    15841 ctcgaccggg ctctcacgcc cgacaggcag gcgcaaggga aggatccggg gagcccctgg
    15901 atgcgaggcg cactcctctc gctcgcggga ggattcggtc tcctcgtcgc gttgaacgcg
    15961 ctcggtgcca tgtaccggac ggcggccatg agctcgcgcc aggggtacga cgaagcccgc
    16021 gcccgcgccg cgctcgacct cgcctcgacc gacgcgctga aggcgggact cctgctcgcc
    16081 gccgcgctct tcgtcatcgg gctcgcccgc cgcggccgtc tctcgcgcgt gggggcatcg
    16141 ctcctcgtga tcctgatcgc ggtcgtggac ctctggccgg tcgaccggaa gatcatcgat
    16201 ccccaggtgg ggaccgcggt cgagtacgac gcgaacttca aggagacgcc ggagatcgcg
    16261 ttcctccgcg ccgatacgac gcagtaccgc gtcttcccgc tccagtggaa cgacagccgc
    16321 ctcgcggcgt tcggcatcgc gtcggtgctg ggatatcacc ccgcgaagcc ctacctctac
    16381 caagcgttcg tcgacacggt cggcatccaa tcatttggca cgctcgagat gctgaacgtg
    16441 aagtacgtgc tcgccgacgg ctacttcccg ccggaggccc aggacaaggc ggcgctccgg
    16501 cacgacgggg aggtcaaggt ctacgagatc ctccacacca tgccgcgcgc gtccgtgatc
    16561 cacgcggtgc ggccggtgcg ggacgacggc gtcgcgctcg cgacgatgcg gaccggcgga
    16621 ttcgatccgc accatgagat cctctggagc ctgccgggag cgccgccgga cgtggcggcc
    16681 ccgtcgacgc ccgactccgt gcgcacgctc cgctacgact tcaacgacat cgaatacttc
    16741 gtctcgacga cggcgcccgg cgtcttcctc accatcgagg agtacgaccc cgactggaag
    16801 gcgttcgtcg acggcaagcc cgtgacgatc gagcgcgtca attacttcat gcgcggcgtg
    16861 ccgctcgagc ccggagtcca tcacgtgcgc taccagtacg agccgaaggc gctcgcggag
    16921 gggatcaggg tctcgatcgc ttcggcggtg gtcgccgccc tgatcgcgct ctggggcgtg
    16981 aagcagctcc tggatgagcg gcgccggcgc cgcaccgcgg cagcgcccgc tcctcccgcc
    17041 acccccgagg gcgccgccgg atgaaggcgc tcatcatcgt tccgacctac aacgagcggg
    17101 agaacctgga ggagctgcta cgccggattt tcgatcagaa gctcccgacc gagatcctgg
    17161 tcgtggacga caactcgccc gacggcaccg gggccctggc ggatgagatg gcggcgcgcg
    17221 atccgcgggt ccacgtgatg caccggccgg gaaagatggg gctcgggtcc gcgtacgtgg
    17281 ccggcttcaa gtgggcgctc gcgcgcgact acgacgcggt cttcgagatg gacgccgatt
    17341 tcagccacaa ccccgacagc ctgggggact tcctcaggga gctcggccac gccgacctcg
    17401 tcctgggctc ccgctacctc cacggggtga cggtggtgaa ctggcccctc tcccggctca
    17461 tcctctccta ttcggccaac ctctacagcc ggatcatcac cgggatgccg gtgaaggacc
    17521 tcacgggggg gttcaagtgt ttccgtagac aggtgctcga ggccatcgac ctggaccggg
    17581 tccggtccga cggatacgcc ttccagatcg agatcaactt caaggcatgg aggaagggat
    17641 tccggatcca ggagattccc atcctgttcg tcgaccgcca agccggcgtg tcgaagatgt
    17701 cccgccggat cgtccgcgag gccgcctgga tggtctggcg cttgcgcctc ctggacctcc
    17761 tgggcgcgct ctagcgggag gggtcctggg cctctcgctc gtcgtcgtcc actacaagac
    17821 ccgcgatcac ttggagcgcc tgttccagtc tctgcgggag gcgcgccccg cgccgctccg
    17881 ggagatcgtg gtcgtgaaca attcggggga gccgctcgac gacctcgcgc gcgcgtccgg
    17941 gtacccgacc cgcgtcctca cgccgggatc gaatctcggc tacgcgcgcg gcgtgaacga
    18001 gggcatccgc gcggcgtccg aggagaacgt gctcctcttg aatccggacg tgcgcgtcct
    18061 gcctggctcg gtggaggcgc tcgagcgcgc ggcggcggag catccccgcg ctggaatcgt
    18121 cgcgccgaag ctcctcgaat cggacggttc gctccagctc tcggcgcggc ggttctacac
    18181 gtggcggacg ctcctccttc ggcgcatgcc gctccccggc gtcgccgcgc ggagccgcga
    18241 ggtacgggat cacctcatgg aggactggga tcacgccgac acccgcgccg tcgactgggt
    18301 gctcggggcg gccatgtacg tgaggcgggg cgcgatgcgc gacgtcggcc tcatggacga
    18361 gcggtacttc ctctacttcg aggacgtcga ctggtgccag cggatgtggc gccatggtta
    18421 cgaagtggtc tactgtgcgg atgcgcggat ggtgcacgag tacgagcggg ccagcgccca
    18481 gatgcgtccg cgctcggtcc gggcccacgc ggccggactc cttcgattca cggagaagtg
    18541 gagcgcgatc ctctacgcca tgagccagca tcgacgccgg atgatccagg tgatcacgct
    18601 cgcgatggac g
//
LOCUS       DASTKC010000196         5357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_160481, whole genome shotgun sequence.
ACCESSION   DASTKC010000196 DASTKC010000000
VERSION     DASTKC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160481"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFR25_08120"
     CDS             complement(<1..578)
                     /locus_tag="VFR25_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941055.1"
                     /translation="MSSGTYRWWVTYAGWEKHGLAEREKLFAMHACDRFRNQASMIAP
                     YGAYVYHLGVRLATLREWQNIPPVPTSAFKSHDLTWIPPGSELVVFETSGTTISRPGR
                     VRLSDTELYDASLLESFRRHLLPDGAVMPAVVFGPTRTEAPHSSLWYMCDQVVSKLAR
                     GGVWIVRDGNPDWDLADRTLEAAVSEGTPVLVL"
     gene            complement(575..1108)
                     /locus_tag="VFR25_08125"
     CDS             complement(575..1108)
                     /locus_tag="VFR25_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HET9941056.1"
                     /translation="MKRDAVAVFGSSQAPPSSPAYSAARELGRELARLGAEIRCGGYG
                     GVMAGVAAGGKEGGGRVVGCTIQWFSASRTPCPDLTEVQEASDLHVRVQCLLQGTRGA
                     IVLPGGIGTMNELFWVWTLRLHGRKDAPDRLVLLGEVWGELLDLLGSRFEFGEQARML
                     VRVARTPAEAAEIAWTR"
     gene            1173..2957
                     /locus_tag="VFR25_08130"
     CDS             1173..2957
                     /locus_tag="VFR25_08130"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET9941057.1"
                     /translation="MGRVNAKPAAGRSRSSRVVRIALAQVNTTVGDLPGNSKRVLEFL
                     DRARGFGAEIVAFPELVLTGYPPEDLLFRPEFIDQNMAALDEVVRATKGITAIIGFAE
                     RAGDLYNSAAIAHDGEIAGVIRKAHLPNYSVFDEFRYFRPGRNPFVLKRGPLTFGVNI
                     CEDIWLPSNPTQLAVYGDAELVINLSSSPFHMGKSVARDRMLATRAMDGVSLVAFVNT
                     VGGQDELVFDGNSRVFGPRGECLARAKAFEEDLVVIDLDLDQVFSARLKDPRSREMSQ
                     REDAGSPLPIVELSPVAEPERKKIDTRPARTPEGAEEIYEALVLGTRDYVRKNDFSHV
                     LLGLSGGIDSALTCSIAVDALGASSVTGVFMPSPFTATESGTDADALAKNLGVQILTL
                     PIATEMEAFKKALAPVFSGMPEDVTEENLQSRIRGNVLMALSNKFGWLLLTTGNKSEY
                     SVGYATLYGDMAGGFAVLKDVPKTLVFDLARHRNAAAGSNGWIPERTITREPTAELRP
                     GQKDTDSLPPYDVLDPILKAYVEEDWEVERIVAMGYDRALVQRVVRMVDRAEYKRRQA
                     PPGIRITPRALGKDRRLPITNRHQPWRD"
     gene            3019..4821
                     /locus_tag="VFR25_08135"
     CDS             3019..4821
                     /locus_tag="VFR25_08135"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET9941058.1"
                     /translation="MQVSAATNLTTNRHLLAWVTEIAQRCQPDAIEWCDGSETERRRL
                     TDRAVEQGVLIPLNQKKRPGCYLHRSDPGDVARVEHLTFICTPTKEEAGPTNHWMAPA
                     EAYAKVGSLLEGSMRGRTMYVVPYVMGPLGSPFSKIGIEVTDSVYVVLSMRVMTRMGK
                     QALDLLGDSDDFSRGVHSMLDINPERRMICHFPQDNAIWSVGSNYGGNVLLGKKCLAL
                     RIGSYLGRRENWMAEHMLILGVESPEGQTTYVAAAFPSACGKTNFAMLIPPRRFDGWK
                     VWTVGDDIAWMRVGADGRLWAINPEAGYFGVAPGTGPKSNPTALRLVERDTIFTNVAM
                     TEDGDVWWEGLTDTPPAGLTDWLGQKYDPKSGKPAAHPNSRFCSPATNNPFLSKRVND
                     PEGVPISALVFGGRRASTIPLVLQSFNWIHGVYMGATMGSETTAAATGKVGVVRRDPM
                     AMLPFCGYNMGDYFCHWLEMRARIPYPPKIFMVNWFRKGANGSFVWPGYGDNMRVLKW
                     IVDRVHGSVGGRETPVGWVPREGDLDLSGMSIASEDFAEATAVRPEEWKEEIRLQGEF
                     FRTLDPYMPRALELQRELLMSSVEMGSPDGRGRS"
     gene            complement(4829..>5357)
                     /locus_tag="VFR25_08140"
     CDS             complement(4829..>5357)
                     /locus_tag="VFR25_08140"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9941059.1"
                     /translation="RLATWSATGQALEVALHQTPMSAIGAAVFKPTTGPLATLLASDG
                     NRLLSQRPGNAAITAVEIDPISPPSEPFLTVSPNPAPGRVRINVRGGWGDDLAFSSSG
                     YGPAVGRFAIEVFDVAGRRVRRLEGDGGAATSISIEWDGRDASGRPVASGRYWLRPVA
                     RGDRMELAVPVILLR"
BASE COUNT          918 a         1838 c         1772 g          829 t
ORIGIN      
        1 agcacgagca ccggggtccc ttcggacacg gcggcctcga gcgtccggtc cgcgagatcc
       61 cagtcggggt tgccgtcgcg cacgatccac acgccgcctc gcgcgagctt cgaaaccacc
      121 tgatcgcaca tgtaccagag ggaggagtgc ggcgcttcgg tccgcgtagg cccgaacacg
      181 acggcgggca tcacggcacc gtccgggagg agatgccggc gaaaggactc gagcagggac
      241 gcgtcgtaga gttccgtatc gctcagccgc acgcgaccgg ggcgggagat ggtcgtgccg
      301 ctcgtttcga agaccacgag ctccgaaccc ggggggatcc aggtgagatc gtgcgatttg
      361 aacgccgatg tcgggaccgg cggaatgttc tgccattccc gcagggtcgc gagacggacc
      421 cctaggtgat agacgtacgc gccgtacggc gcgatcatgg acgcctggtt gcggaaacga
      481 tcgcacgcgt gcatcgcgaa aagcttctcc cgctctgcga ggccgtgctt ctcccatcca
      541 gcgtaggtga cccaccatcg gtatgtgcca gagctcatcg agtccatgcg atctccgccg
      601 cctcggcggg cgtgcgcgcg acgcgcacga gcatccgcgc ctgctcgccg aactcaaaac
      661 ggctgccgag gaggtcaagc agctcgcccc acacctcgcc caagagcacg agccggtccg
      721 gtgcgtcctt ccgtccatgg agacgaaggg tccacaccca gaagagctcg ttcatggttc
      781 cgatcccgcc tgggagaacg atcgcgccgc gggtcccttg aaggaggcac tgcacgcgga
      841 cgtgaaggtc tgatgcttcc tgtacctcgg tgagatccgg acacggagtg cgggaggccg
      901 agaaccactg gatcgtacag ccgacgacgc gcccgccgcc ctccttgccc ccggccgcga
      961 cccccgccat gacgccgccg tacccgccgc agcggatctc ggcgccgagc cgagccagct
     1021 cgcgaccgag ctcgcgggcg gcgctgtagg cgggcgacga gggcggcgcc tggctgctgc
     1081 cgaagaccgc gaccgcatcc cgtttcatgg cctgagtata aggtggaggc ggtcgccggc
     1141 gcgcccgcca gaaccgcgcc gatcgtgcta ccatggggcg cgtgaacgcc aagccggcgg
     1201 ccggccggag ccgctcctcc cgcgtcgttc gaatcgccct agcccaggtc aacacaaccg
     1261 tcggggactt gcccggaaac tccaagcggg tgctcgagtt cctcgaccgc gcccggggct
     1321 ttggggcgga gatcgtggcc ttccccgagc tcgtgctgac cggctatccg cccgaggatc
     1381 tcctcttccg gcccgagttc atcgaccaga acatggccgc gctcgacgag gtggtgcgcg
     1441 cgaccaaggg catcaccgcc atcatcggct tcgccgagcg cgccggggat ctctacaact
     1501 cggccgcgat cgcgcacgac ggcgagatcg cgggggtcat ccggaaagcg cacctcccga
     1561 actattccgt cttcgacgaa ttccgctact tccgcccggg acggaatccc ttcgtcttga
     1621 agcgcgggcc gctcaccttc ggcgtgaaca tctgcgagga catctggctc ccgtccaacc
     1681 cgacccagct cgccgtctac ggcgacgcgg agctcgtgat caacctctcc tcctccccgt
     1741 ttcacatggg caagagcgtc gcgcgcgacc gcatgctcgc gacgcgggcc atggacggcg
     1801 tgtcgctggt cgcgttcgtg aacacggtgg gcgggcagga cgagctcgtc ttcgacggga
     1861 acagccgcgt cttcggaccg cgcggcgagt gtctcgcccg cgcgaaggcg ttcgaggagg
     1921 acctggtcgt gatcgatctc gacctggacc aggtcttcag cgctcgactc aaagatcccc
     1981 gctcgcgcga gatgtcgcag cgcgaggatg ccgggtcgcc gcttcccatc gtggagctga
     2041 gccccgtggc cgagcccgag cggaagaaga tcgacacacg cccggcgcgc acgcccgagg
     2101 gggcggaaga gatctacgag gcgctcgtgc tcgggacgcg cgactacgtc cggaagaacg
     2161 acttcagcca cgtcctcctc ggcctgagcg gcggcatcga ttcggcgctc acctgctcca
     2221 tcgcggtcga cgcgctcggc gcatcgtcgg tcacgggggt gttcatgccc tcgccgttca
     2281 ccgcgacgga gagcggcacg gacgcggacg cgctcgcgaa gaatctcggc gtccagatcc
     2341 tcacgcttcc gatcgcgacg gagatggagg ccttcaagaa agcgctcgca cccgtcttct
     2401 ccggcatgcc cgaagacgtg accgaggaaa atctccagtc gcgcatccgc ggcaacgtct
     2461 tgatggcgct ctccaacaag ttcggctggc tcctcctcac gaccggcaac aagagcgagt
     2521 actcggtcgg ctacgccacg ctctacggcg acatggcggg agggttcgcg gttttgaagg
     2581 atgtgccgaa gacgctcgtg ttcgacctgg ctcgacatcg gaacgccgcc gcgggatcga
     2641 atggatggat ccccgagcgc acgatcaccc gcgagccgac tgcggaattg cgcccgggac
     2701 aaaaagatac cgactccctt ccgccgtacg acgtgctcga cccgatcttg aaggcgtacg
     2761 tcgaggagga ctgggaggtg gagcggatcg tcgcgatggg ctacgatcgt gccctcgtcc
     2821 agcgcgtcgt gcgtatggtc gaccgggcgg aatacaagcg ccgtcaggcg cctccaggca
     2881 tccggatcac cccgcgagcc ctcggcaagg atcgccggct gcccatcacc aaccgccacc
     2941 agccgtggcg ggactgacac cgtgacgatc acccgtggac cttcgcgtga aggggggcag
     3001 cgcaacgtga atcccgatgt gcaagtgtcg gccgccacga atctgacgac caaccgccac
     3061 ctgctcgcgt gggtgacgga aatcgcccag cgctgccagc ccgacgcgat cgagtggtgc
     3121 gacggctccg agacggagcg tcgccggctc accgatcgcg cggtcgagca gggcgtgctg
     3181 atcccgctca accagaagaa gcgccccggc tgctatctcc accgctccga tcccggcgac
     3241 gtcgcgcgcg tggagcacct caccttcatc tgcactccga cgaaggaaga ggcgggaccc
     3301 acgaaccact ggatggctcc cgccgaggcc tacgccaagg tcgggagtct cctcgaaggc
     3361 tccatgcgcg ggcggacgat gtacgtcgtg ccctacgtca tgggaccgct cggctcgccg
     3421 ttctcgaaga tcgggatcga ggtgacggac agcgtgtacg tcgtcctctc catgcgcgtg
     3481 atgactcgca tggggaagca ggcgctcgac ctcctcggcg attcggacga tttcagccgc
     3541 ggcgtccact cgatgctcga catcaacccc gagcgccgca tgatctgcca ctttccccag
     3601 gacaacgcga tctggtccgt cggcagcaac tacggcggga acgtcctcct cggaaagaag
     3661 tgcctcgccc tccggatcgg ctcctatctg ggccggcgcg agaactggat ggccgagcac
     3721 atgctgatcc tcggcgtcga gagtcccgag ggacagacga cgtacgtcgc ggccgcgttc
     3781 ccgagcgcat gcggcaagac caacttcgcc atgctgatcc cgccgcggcg cttcgacgga
     3841 tggaaggtct ggaccgtggg cgacgacatt gcgtggatgc gggtcggcgc cgacgggcgg
     3901 ctctgggcaa tcaatcccga ggcgggctac ttcggcgtgg cgccgggcac tggccccaag
     3961 tcgaacccga ccgccttgag gctcgtcgag cgcgacacca tcttcacgaa cgtcgcgatg
     4021 accgaggacg gagacgtctg gtgggaaggg ctcaccgaca cgccgcccgc ggggctcacg
     4081 gactggctgg gtcagaagta cgatcccaag tcgggcaagc cggcggcgca tcccaacagc
     4141 cgcttttgct ctcccgcgac caacaaccca ttcctctcga agcgcgtgaa cgatcccgag
     4201 ggcgtgccga tctccgcgct cgtcttcggc ggccggcgcg cgtccacgat cccgctcgtg
     4261 ctccagtcgt tcaactggat ccacggcgtc tacatgggcg ccacgatggg ctcggaaacg
     4321 accgcggccg cgaccggcaa ggtcggcgtg gtgcggcgcg atccgatggc gatgctcccc
     4381 ttctgcggct acaacatggg ggactacttc tgccactggc tcgagatgcg cgcgcggatt
     4441 ccctacccac cgaagatctt catggtgaac tggttccgga agggcgcgaa cggctccttc
     4501 gtctggccgg gctacggcga caacatgcga gtgttgaagt ggatcgtgga tcgcgtgcac
     4561 ggctcggtgg gcgggcgcga gacgccggtc ggctgggtgc cgcgcgaagg cgatctcgat
     4621 ctctccggca tgtcgatcgc gtccgaggat ttcgccgaag cgactgcggt gcgccccgag
     4681 gagtggaagg aagagatccg cctccagggc gagttcttcc ggacgctcga cccctatatg
     4741 ccgcgcgcgc tcgagctgca gcgcgaattg ctcatgagct cggtggagat ggggagtccg
     4801 gacgggcggg ggcgttctta gagaggcatc agcgaagcag gatcaccggc accgccaact
     4861 ccatcctgtc tccacgcgcc accggccgca gccagtagcg tcccgaagcc acggggcgcc
     4921 cgctcgcatc ccttccatcc cactcgatcg atatggatgt tgccgcgccg ccatcgccct
     4981 cgagtcgcct gacccttctt cccgccacgt cgaagacctc gatcgcgaac cgtccaaccg
     5041 ccggaccgta gccgctggaa gagaaggcga gatcatcgcc ccacccgccc cgtacgttga
     5101 tccgtacgcg tcccggggct ggattcgggg agacggtgag gaatggctcg gacggcggcg
     5161 atatgggatc gatctcgacc gccgtgatcg cggcgttgcc gggccgttgc gagagaagcc
     5221 ggttgccgtc gctcgcgagg agcgtggcca agggacccgt ggtcggtttg aacacggcgg
     5281 cgccgatcgc gctcatcgga gtctggtgca aggcaacctc gagcgcctgg cccgtcgcgg
     5341 accacgtcgc gagacgc
//
LOCUS       DASTKC010000197         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1107200, whole genome shotgun sequence.
ACCESSION   DASTKC010000197 DASTKC010000000
VERSION     DASTKC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107200"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VFR25_08145"
     CDS             <1..1503
                     /locus_tag="VFR25_08145"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET9941060.1"
                     /translation="DSVMAIGGSLLHRADIGILAVSLDKGDTLVALSESRRLIPGSNA
                     KLFTNGAFLVRFGTDYRRATTIDARGKTSLKDSGRRVRLDGDLVLRGSGMPDVTQILS
                     PGSRGLLDSLAYLLHEGGLERFEGTIWVDGSLFATEPYAPGWAMEDYVYAYGAPVNAI
                     LANGNAATVVATSTGKGNEVTLALDPPEAPLRVRGKVDVVASGSGRLDVSRAFGSRMV
                     DVWGTVPRGGVTKRQVAIPDPDSTAGLYLLGSLRRAGIEVKAQVRVLPPAGSPEWVRI
                     GALARKDGSMEAAFTPDPGRWKSVKKDATVPVVSLLSPPAAQMVSAVNELSLNGETEA
                     LLRNLVDGTGKERIPALSRLYEIVARSGVDTLDLSLVDGSGLSPMDLVTPRAVVTWLS
                     WLDREPSVGPAFREGLAGPGLLGTLRNRFGGAEGGVQIRGKTGTLTNVSALSGYVTTE
                     SGERIVFSSIANGNRGTVAPARDFEDRFVRLLSRYKREAIPVRRPGTIPR"
     gene            1500..2411
                     /locus_tag="VFR25_08150"
     CDS             1500..2411
                     /locus_tag="VFR25_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET9941061.1"
                     /translation="MIAPPRLRPGDTIGVVAPSGPVQRGAVLAGARALEQAGFRVRLA
                     RHLYDKRGHLAGSDAARAADLNRMLADPDVRCVLMARGGYGLLRLAPEVDWAAMRRDP
                     KIYAGFSDATFLHLGFALHANVRTLHGPNLQGLKGGEARRWLAWVTQPKPAESVRTLR
                     ARASLAAGAAVEGRVLGGNLTLVHYAIGIGTLPTFRNAILFLEEVNEPPYKVDGMLTH
                     LALAGALRGVRGVALGDFTKCVPRPHRRELPLKAVLTDHLRPLGGPILKGLAAGHGPK
                     NTPLPLGARAVLDPRRATLIYEEGLVS"
BASE COUNT          365 a          880 c          860 g          363 t
ORIGIN      
        1 gactcggtca tggcgatcgg cggatcgctc ctccatcggg ccgacatcgg gatcctcgcg
       61 gtgtcgctcg acaaggggga caccctcgtc gccctgagcg agagccgacg gctcatcccc
      121 gggagcaatg cgaagctctt cacgaacggc gccttcctgg tccggttcgg caccgactac
      181 cggcgcgcga ccacgatcga cgcccggggc aagacgtcgc tcaaggactc ggggcgccgc
      241 gtccgcctgg acggggacct cgtgctccgg ggaagcggaa tgccggacgt gacccagatc
      301 ctgagccccg ggtcgcgggg acttctcgac tcgctcgcgt atctcctcca cgagggcggg
      361 ctggagcgct tcgaagggac gatctgggtg gacggctcgc tcttcgccac ggagccctac
      421 gcgccgggct gggcgatgga ggactacgtc tacgcctacg gcgcgccggt gaatgccatc
      481 ctcgccaacg gaaatgccgc gacggtcgtc gccacgagca cggggaaggg aaacgaggtg
      541 accctcgcgc tcgatcctcc cgaggccccg ctccgcgtgc gggggaaggt cgacgtcgtc
      601 gcgtccggct cgggacgtct cgacgtgagc cgcgccttcg ggtcccgcat ggtggacgtc
      661 tgggggacgg tgccgcgcgg gggcgtgacg aaaaggcagg tcgcgattcc cgatcccgac
      721 tcgacggcgg ggctctatct cctcggctcg ctgcgccgcg cggggatcga ggtgaaggct
      781 caggtccgcg tgcttcctcc cgcgggatcg cccgaatggg tgcgcatcgg cgcgctcgca
      841 cggaaggacg ggagcatgga ggcggcgttc acgccagatc cggggcggtg gaagagcgtg
      901 aagaaggacg cgacggtgcc cgtcgtgtcc ctcctctcgc cccccgcggc acagatggtg
      961 agcgcggtga acgagctcag cctgaacgga gagacggaag cgctcctcag gaatctcgtc
     1021 gacggaacgg ggaaggagcg cattcccgca ctgtcgcggc tctacgagat cgtggcgcgc
     1081 tccggcgtgg acacgctcga tctctcgctc gtcgatgggt cgggcctctc gcccatggat
     1141 ctcgtgactc cgcgcgcggt cgtgacctgg ctctcgtggc tcgatcgcga gccctcggtg
     1201 gggcccgcgt ttcgcgaggg gctcgcggga ccgggcctcc tggggacgct ccggaaccgc
     1261 ttcggcggcg cggaaggggg cgtccagatc cggggtaaga ccggcacgct gaccaacgtg
     1321 agcgccctct ccgggtatgt caccaccgag tcgggcgagc ggatcgtctt ctcgtcgatc
     1381 gcgaacggca atcgggggac cgtcgcgccg gcgcgcgatt tcgaggatcg cttcgtccgc
     1441 ctcttgagcc gctacaagcg cgaggcgatc cccgtgcggc gtcccggaac cattccgcga
     1501 tgatcgcgcc ccccaggctc cgtcccggcg acaccatcgg ggtcgtggct ccatccggcc
     1561 ccgtgcagcg aggcgccgtc ctggccggcg cgcgcgccct ggagcaggcg ggctttcgcg
     1621 tgcgcctcgc gcgccacctc tacgacaagc gcgggcatct cgccggcagc gacgcagccc
     1681 gcgccgccga cctcaatcgc atgctggccg atcccgacgt gcgctgcgtg ctcatggcgc
     1741 gcggcggcta cggcctcctc cggctcgcgc ccgaggtgga ctgggccgcg atgcgccgcg
     1801 acccgaagat ctacgccggc ttctccgacg cgaccttcct gcacctcggc tttgcgcttc
     1861 acgccaacgt gcgcacgctc cacggcccca atctccaggg gttgaagggg ggcgaggccc
     1921 ggcgctggct cgcctgggtc acacagccga aacccgcgga atcggtgcgc acgctccgcg
     1981 cccgagccag cctcgccgct ggggccgccg tggagggtag ggtgctcggc ggcaatctga
     2041 cgctcgtcca ctacgcgatc gggatcggaa cgctcccgac gttccgcaac gccatcctct
     2101 tcctcgaaga agtgaacgag cccccgtaca aggtggacgg catgctcacc cacctggcgc
     2161 tggcaggagc cctgcgcgga gtccgcggcg tcgccctagg cgacttcacg aagtgcgtcc
     2221 cgcgccccca ccgccgcgag ctccccctga aggcggtcct aacggaccac ctcaggccgc
     2281 taggcggccc catcctcaag ggtctcgccg caggtcacgg tccgaaaaac acgcccctcc
     2341 cgttgggtgc ccgcgccgtc ctggacccaa gacgggccac gctgatctat gaagagggtc
     2401 tggtctccta aaacccgcga accgtggggg tagacgaagg gtccggtaca agcggaggac
     2461 gcctcaca
//
LOCUS       DASTKC010000198        41091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1107704, whole genome shotgun sequence.
ACCESSION   DASTKC010000198 DASTKC010000000
VERSION     DASTKC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 41091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41091
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107704"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VFR25_08155"
     CDS             <1..1113
                     /locus_tag="VFR25_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941062.1"
                     /translation="EALQFYQSWRVFAAQEGREGDMLLRIYDVTALMGRTQDALKGYN
                     DLVNKFPRTAVAYEAQFQIGYLYETAVQDLDAAGREYDKLKSQPTSEFQQQAMRRSQS
                     LAAIRTYRNSLASDSTGARAHAAFMMAELNYFQLGKPDSAIVQYQRVEMEFPHSVYAA
                     KAAYARLWIATQDKGDTLEAMRIADMMGQRYRGTPYAESGLYLWKQWSGRTDERTALL
                     DSLLANPDTSQVVVLEPELADSTFASPDSTLIRPQTYAMTPADSARMDSLRQHALEIR
                     EQYMREHGDKKRPKPQQQVRPQGPTPQVQTPTPSAPNAAGGAAAAGAAAAGAAAAGGS
                     APADSSTNPTPPPSTTAAPDSTARPDSTTHEDDTGP"
     gene            1110..1916
                     /locus_tag="VFR25_08160"
     CDS             1110..1916
                     /locus_tag="VFR25_08160"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET9941063.1"
                     /translation="MNLVSCSVVSNHELNPGNFLITLKVPRGFARPQPGQFVHLRVGD
                     GLEPLLRRPYSLEGFMEKGALRAVRIYYSVVGRGSGVLARQEKGTKLDLIGPLGIGFA
                     PRPRRLPILVAGGRGVAPLLFLSRRLRERKRPYVFLYGARSKTELYGVREVRGGRVHL
                     ATDDGSIGYKGSVMDLLAREWSESGHTPLTAEIFTCGPHGLLHAISDFARDRGIRCEA
                     SLEGPMACAVGACRGCPVPLLPGVDSGRYPAMCLEGPVMDATVVDWARLP"
     gene            1913..2893
                     /locus_tag="VFR25_08165"
     CDS             1913..2893
                     /locus_tag="VFR25_08165"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET9941064.1"
                     /translation="MKPSLEVRIGGLTLRNPVLVASGIIGYGREYERLTDLSRIGGIV
                     TKTVTLRPRAGNPPPRVCETPSGMLNSIGIENPGLSGYLEQKVPILRELPCAAIVSVE
                     GEDVGEFCELVRGVDGSGAAHAIEVNISCPNVGPHGMRYSTDAGMAREVMSAIRPLTK
                     LPLIAKLTPNVTRIAEIAQACEDCGADAVSLVNTFVGMAVDARTRKPILGTVLGGLSG
                     PAIKPLALAKLWETVQAVDIPVVGMGGIARPTDAVEFLLTGATAVEVGTAMFANPSLP
                     EECVDAIEEAVRLAAGTSVDDLIGALEVPEGRGVIRAGRGARREPGRHAS"
     gene            2890..3618
                     /gene="pyrF"
                     /locus_tag="VFR25_08170"
     CDS             2890..3618
                     /gene="pyrF"
                     /locus_tag="VFR25_08170"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9941065.1"
                     /translation="MSLRDRLIVALDMPDLEHAVAHVDRLGAGVLWYKVGLQLFYASG
                     RRAVETIAGRGKNVFLDLKFHDIPATVEKAIRALDGLPISLLTVHAAGGPAMLEAAAR
                     AARSIGSQPTVLGVTVLTSLDGNELPGVWDPGTDLEAKVLAFAKLCADAGVGGVVASP
                     RELAALRRAHPAPFRIVTPGIRGPEDRSHDQKRTLSIGEAFALGADHIVVGRPILEAA
                     DPKAVLAAYEAAAGLHSSTERMIG"
     gene            3615..4565
                     /locus_tag="VFR25_08175"
     CDS             3615..4565
                     /locus_tag="VFR25_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9941066.1"
                     /translation="MKVLVTGITGFAGSHLVDYLLTIPGVEISGILRWRSRTENIEHF
                     MDKVRLVECDLRDASSVRDAIDELRPDRIFHLAAQSFVPTSWTAPTESLVTNVIGQLN
                     LFEAVKKLGIKPRIQLACSSEQYGMVYEDELPIRETNPLRPLSPYAVSKVGQDALGYQ
                     YWMSFGIPIVRTRGFNHEGPRRGPVFVCSDFARQVADIEKGRKAPVIRVGNLDARRDF
                     TDVRDIVRGYWLSLEKAEMGEVYNLCSGKDWTIRAMLDLLLSMSNVKVKVEQDPARMR
                     PSDVPVLLGDASKFHKATGWKPEIPFEQTLRDLLEYWRAR"
     gene            4569..5543
                     /locus_tag="VFR25_08180"
     CDS             4569..5543
                     /locus_tag="VFR25_08180"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9941067.1"
                     /translation="MRLFITGVAGFAGGHLLEHLAEVGGFEVHGADHAPLEAHPNAAA
                     LRARLASYGALDITSADAVDSWLTPRKPDALIHLAAQASGADALEKPVATYDVNVMGT
                     LHVLEALRRTAPSARVLVTGSADVYGSGAAGGRIREDAPLRPANPYAGSKAAQDVIAE
                     IYAATFHLPVIRTRTFSHTGPGQRPRFALAGFADQLARIDAGFAPPEIRVGNLDVTRE
                     YGDVRDVVRAYVLLLQRGEPGAAYNVATGQGHKLRDLLDRLIEISGVAATVVVDPSRV
                     RSRDADHLVGDPAKLVSATGWKPLRSIEQTLSDLYRDARDRVLSQAKA"
     gene            5554..6870
                     /locus_tag="VFR25_08185"
     CDS             5554..6870
                     /locus_tag="VFR25_08185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET9941068.1"
                     /translation="MRITIVGTGYVGLVTGAGFADFGNQVLCVDVDRAKIETLERGQL
                     PFYEPGLDDLIARNVRERRLRFGLSLEEATRWGEAIFICVGTPQGEDGRADLKYVDAA
                     ARVIGRAMNGYRLIVQKSTVPVGTAERVRGIVKKNAKRGMKFDVASNPEFLREGTAVE
                     NWMRPDRVVIGADSTRARQLLREIYSPLYLIETPMVLTDVRTAELIKYAANAFLATKI
                     SFINEMANLSEALGADVKDVAKAMGLDRRIGPKFLHPGPGFGGSCLPKDTRAMTGFAK
                     KAGVKLRIVQAAVETNDAQQDIAVGKVMDALPGARKSRTAAILGLSYKPDTDDLREAP
                     ALHIIRALLKRGVRVRAYDPVVREGAAGVPAGITFAENAYDAAKGADALVIVTEWNEF
                     RRLDLARIKRAMRRRAIVDLRNIYDPASVRKLGFDYTSVGRPNEAD"
     gene            6857..7318
                     /locus_tag="VFR25_08190"
     CDS             6857..7318
                     /locus_tag="VFR25_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET9941069.1"
                     /translation="MKLIDGVVVKPLKVIADERGYLMEMMRKDDPFFQRFGQTYVSVA
                     YPGVVKGWHYHKIQTDHFVIVKGMMKVVMYDDREKSPTKGVVNEFFMGERNPILVTIP
                     PGVWHGMKGIGSEPAMLVNCPTEPYNYKEPDEYRCDPHKNDIPYDWAQKDG"
     gene            7311..8342
                     /gene="rfbB"
                     /locus_tag="VFR25_08195"
     CDS             7311..8342
                     /gene="rfbB"
                     /locus_tag="VFR25_08195"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET9941070.1"
                     /translation="MAERLDLNRSRLLVTGGAGFIGSNFVHHLRQSYPQAAITVLDKL
                     TYAGNLANLEVVKDDPGYRFVKGDICDRKVVAEAMEGCDFVVNFAAETHVDRSIDHAE
                     DFVLTDTYGVWVLLEEARKRKLTRFLQVSTDEVYGEILDGEATEESPLLARNPYAASK
                     IGGDRLAYSYFATFQTPTIVTRCSNNYGPYQYPEKLIPLFVTNALEDKTLPVYGSGRN
                     TRDWIHVLDHCRAITAILESPGVEGHTFNIAGNNERSVNQIADLILKRLGKPESLLKS
                     VTDRPGHDRRYAIDASKLERMTGFKPQVEFATGITETIDWYKTHRSWWEAIRSGEFRH
                     YYEKMYGAR"
     gene            8367..9848
                     /locus_tag="VFR25_08200"
     CDS             8367..9848
                     /locus_tag="VFR25_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET9941071.1"
                     /translation="MRRLVRSTAILGFGSIATIVAAILRAKILAAWLGPEGTGALAQL
                     AAFTAVLVPFATLGAGSGVITMIADARARGDLDRVRRITSTARSLTWAAGIALAVLSL
                     ALSPWITDAVYRDRAFVWAILIGAATIPLSGVASIQISMLQGYGAVRAMAILNGLVAA
                     TTIATIVPMAWFFGVKGAVTQLIVLAVAQVLLGNRLLKPYVPSAPAAAESTVDRSLLR
                     PIARFGGSALLVGLSSTLTVLILRSIIVDKLGLGQNGIYQVCVGVSGLTMPLILNSIT
                     ATVWPEIAAQPTDEGAGVPMRSGIRLCFLLTTGLAAALMVGAPVWVPLLYSTKFRPAL
                     ELLPYQFLGDYFRAAAWMFGIWLVPRNRLRPWVLFDVVYGLVILGAFLFLVDRIGVRA
                     TVIAYMLAHVSHAVLHYGLARRAIGFRLGPDNRVLLLASLALLLLLLVVRPEDTPGMV
                     IGAILTAAWAALVVRPREWRGLRDMALRQIRRFAPGPGRPGGE"
     gene            9905..11503
                     /locus_tag="VFR25_08205"
     CDS             9905..11503
                     /locus_tag="VFR25_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET9941072.1"
                     /translation="MTQIRSISHRIAPRFVLGLTACCAGLLVLTTAWAQTPGAGVSDD
                     AARAAQIDTPIEGRVDPTTYRIGPGDELALRYSDLLEPKILRVAPSGELLLPDVGTFH
                     VAGLTLAETQARLKEALKPYIKGKGFVLALSRPRRFRMPVLGDVERPGSVTLQAPVRA
                     SEAIAAAGGVTATGARRGIEVRRSSDTLRVDLVQYTRGGDLASNPLVFETDVIYVPPA
                     RKRIEVLGAVPHPGAYDFVPGDRLSTAIALGGGTLADAAPEAAELARVRADGTRSVEK
                     IAIAQALEAPGGDADPVLAEGDRVFIPSRSHYLKGAHVEVEGEVARPGPYPITEGVDR
                     IRSVLERAGGFTDQADRAAMRVERMVKDEEPDSVFLRIALQQEELLAGSDRSYVIAKS
                     RERQAISAHVGVLLEAGDERGDLPLRDLDRIVVPKRVPYVSVQGEVRAPGYVPYREGW
                     KVDDYVKAAGGTTSRAYKSRTRVTLSLTGRPVAAEDVGALRPGDQVLIPSKPDRNPWG
                     TIRDVIGVVAAASAIVIAVEAVNQ"
     gene            11506..12549
                     /locus_tag="VFR25_08210"
     CDS             11506..12549
                     /locus_tag="VFR25_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HET9941073.1"
                     /translation="MALARRDREDFMGVVRARRRFLALNVLATTLVALGISLILPKWY
                     TGRAVLLPPVEDDATSALSMLMPKSLGSVRLPGAPTMADVFVAVLKSRSVADRLVSRF
                     DLVRRYHLKDEERAVKELSSHVAFRLGDEGTITIAVEDKDPKTAAAMANAYVEELDRF
                     NVETRTSSAKRTRAFIDERLTLAKQDLANAEDRMRAYQQQHKLPALSPSAQGDTDVGA
                     NLMAQKIALEVKLQVLQQSLAESSEEVRRVRQELAAIDRQVGGLPQAGIEIMRLWRDV
                     KVQEQVFELLTAQLEEARIRETRDTPTVQVLDHAVVPLHKSRPKRALVVLAGFVIGLA
                     GSLTWVLLHDRPR"
     gene            12519..13946
                     /locus_tag="VFR25_08215"
     CDS             12519..13946
                     /locus_tag="VFR25_08215"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9941074.1"
                     /translation="MGASARPAALAAPPSRLSLGALAWIALAELLLLLLGWKLGSLAW
                     PLAGAFAVAFALVAYRSPETAWILVWIATPFSREIVVVGGAAVSVPTEPMMAVAVGVW
                     ILRRWPWREGSAGPRSITRPLFVLAAIALVSIAVSRFPFEGMKAWMMAALYVVFGYLY
                     FVSTPFDARRAKLWMALGVLLASLLSIYASYRVLSAGIGVRSAYGAARPFFPEHGTFS
                     AYLAFFLPVAIVQSLARERGRLLWTLGSAAILVGIVLSFTRAAWLSAVFVLPLLLVSW
                     MLGRGGASRLWLPLTIVALVSAWIGTSRVADPFLGHARTIVNSGNVSNLERVNRWMAA
                     AEMIRARPLTGVGYGVYPEAYHQYRRKSIVTDQALTTFGVHNELLRILSETGWPGLAA
                     CLWFVGSVAVVGFRTFLDRPGSAEGRLALGLILGLTTYAVHSLFNSYLGIDKITTPFW
                     VFLGMTAALGSARTSNAAPDEKDAA"
     gene            14056..15960
                     /locus_tag="VFR25_08220"
     CDS             14056..15960
                     /locus_tag="VFR25_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941075.1"
                     /translation="MVLGFALVLASLLVVSVEVVGALGLLIAPIALPGVASALALALL
                     ISTRVVPREPSPAGQPAASASTGTGPGPVHVVDRVASREARAIGLSAVVLLLTWLPLA
                     WSRLALPPVSWDALTYHLAFPAGWLERGDLATTVPASGDAANTFYPLVGQMLLAWNLV
                     STGTDRWTVIAQLPFLLAGALAVSGIARHLGATRGPALLAGLLFAATPVALRQSVEVM
                     LDVEQAALFAMAAYFALRSRSGSPGDAILGWLAVALLAGLKYSGAVLAVPLVLLLVVS
                     GLRSGRSGVASLGVGLIAALAVGGFAYVRNAVTCGNPFMPLEVTVGGHVLWPGSSHAA
                     AYFGPNAPRLGWRELLLSPRCVLEMGVVFLPALAILAIGALALRGSKERMWLAWIGVA
                     AFALSALVLPFREHRYFLPVVAAACALAPGLLRGPRSRTWFARAVPVVVLLQAPVSLA
                     YVAKDVMVLGPAGAAHAFGHGASTGYESSRYERWLSYWSTRHEWEDRAHARADLRDMA
                     AAWAWIAGHTDLAPAIVAYAGANTPYPFTGEGSRNRVLFVPRSGAADGTTYRWGKPPA
                     SVQEPDSTAWRSNVRALGAAYLCVVRLDARYGGSGAFPIEDEWARADTDGFSPLWTSE
                     NARIYGVRVR"
     gene            complement(15939..16934)
                     /locus_tag="VFR25_08225"
     CDS             complement(15939..16934)
                     /locus_tag="VFR25_08225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET9941076.1"
                     /translation="MPADPTALRFTVVVPTLGQDHRLNTLLDALASQTLDRSRWDLVV
                     SFDGAEPSTGIARRLDAMSATTVSNRERRGPGAARNAGAARAMGDYLAFTEDDCLPSS
                     DWLALAAQRLERDPGTDVLEGATVLPDGSPARRREPGELTFLPTNLIVLRSFFERIGG
                     YCEQFFDPERGVYFREDSDFGFTALEQGARAAFDPAIRVEHPREHPGWLDPIRWAQRY
                     EMDPLLARRHPAAFRERIEIARLGPFRMRRPFVRACTGFIIAAVIALTLALQGEPGLA
                     VWFAALAGALLLVVWSKWRFDPRRIPLVPAVPFVLVAALLRGRRRASLAQRTRTP"
     gene            complement(16947..17909)
                     /locus_tag="VFR25_08230"
     CDS             complement(16947..17909)
                     /locus_tag="VFR25_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HET9941077.1"
                     /translation="MNKDTRIVVTGGAGFLGRHVMAELEAQGYRNAVTFRSADYDLTH
                     ESSVERMLEDLKPQAILHLAASVGGIGANRRYPGTYYYRNLMMGSLLMEHARNAGVGR
                     FLSVGTICSYPKFTEVPFREENLWNGYPEETNAPYGLAKKMLLVQSQAYRQEFGFDAS
                     NVLVVNLYGPYDNFDPESSHVIPALVRKCVEAVETGADTIEVWGTGKATREFLYVEDA
                     ARGLVAALERLEGSDPVNLGAGFEISIRDLAETIARHAGFKGRLLWDPTKPDGQPRRC
                     LDTEKAAALLGWRATMPFDEGLARTVAFFRDHVRALERVQPAAR"
     gene            complement(17965..18522)
                     /locus_tag="VFR25_08235"
     CDS             complement(17965..18522)
                     /locus_tag="VFR25_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9941078.1"
                     /translation="MRVLNLGCGHAKRDFPEASQASEIVGVDHSPASQADVIHDLDRM
                     PYPLPSDSFDLIVMQDVIEHLEDIPAVMAEVHRVARHGALVRIRTPHFSSWYAYNDPT
                     HRHVLGAFAFDGFLAASPNVLYTSARFERVRRRILFPRLWRMTGAAALANRFPARWEQ
                     LFAFVVRAENLEFELRAVKEPAASP"
     gene            18601..19797
                     /locus_tag="VFR25_08240"
     CDS             18601..19797
                     /locus_tag="VFR25_08240"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9941079.1"
                     /translation="MSLALERGAALSFLLLAAALPWSIAPMSIAVALSGALTLACWWR
                     PGGARWVRTPVDPAAIVWLIALGIAALAGEDPAGSVNRVTKGFLLAIVPLAAYHGRDP
                     KLARRAIAVLLVSAAVATVFALVKFAMQGGAFPVRVRGAVGHPLTYGGQAMLLAVLAG
                     AILLRSRDRRWKGAAGALLLLLAPALLGSYTRSAWIGTLVGFAILIGFTRARWLPALL
                     GALLVLVLVLPGGYRERALSVFHPHGVWNRERIILWDAGWRMFRDHPVTGVGLEDLKP
                     WVARYRSPEAHEPPHGHMHNVYLQVLVTMGVIGFAAFVYLVTALWRTAARRLRVDARS
                     LDADPFGMALRIGTVAALAGFLVAGLFEWNFGDEELLDFLFTLIGLAWAASAWRHVRP
                     VAPPSS"
     gene            19887..20957
                     /locus_tag="VFR25_08245"
     CDS             19887..20957
                     /locus_tag="VFR25_08245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9941080.1"
                     /translation="MISFAQEAPGPTPEPWLGLGIDVKTVPHRPPSKPVALAQGLLGK
                     LPYTLARYRSAAFARVVDECVRTLRPAFALANNLHTASYVDRLDGVPLVLREQNLEHL
                     WMSRYAEESLGGLARVYAGIQARRLKAAERAFCRAAALTLAIQDLERDALRALAPGAW
                     IETLPVGIDLDRFGEPRTADPPIVLLAGAFGWPPTASGAIRFLKDGWPRVRAAVPGAR
                     LRVVGKSQPDDLVRAARALGADVVTVVPSMAEEMARASVMVVPLWSGAGARVKIMEAL
                     AARLPVVATPIGAEGLGLEQGRHHVEGESAGELGDRTGALLRDPERRASLAREGRAYA
                     EAHWSQAAVARLQNELVGRIAR"
     gene            20954..22087
                     /locus_tag="VFR25_08250"
     CDS             20954..22087
                     /locus_tag="VFR25_08250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9941081.1"
                     /translation="MNEYVLFFLSAVVSVVVNALGRLRGRSEPSRILVVKLDHLGDVI
                     LATPAIRALHMRYPKAVIDVLVHPGGTAAVAHHPGVHSVLTYRSPRFSRDGTADDSSR
                     LHEVARTRYDTVVELRGDGRTLLLPFRTGARRRVDRGTVRLRNKALRVLGRAPTLHEV
                     ETNLEIVRPLVGAPAARPRVEVDPGPEARASLTRTLEERAVPAGARLVVLHPGAAWRP
                     RAWRPERFGAVADWILGHYDAQVVLVGSEDERDVEDLVRRSMREDRALAMTGSLTLEE
                     LTALLGRSALFIGNDSGPAHLAAACGVPSIVLFGPQDPRRFGPWSERTQVLHHPVACF
                     PCRQTVCVMAEDPCVNRNEVADVIARARDLLGPPERVQAAARG"
     gene            22090..23487
                     /locus_tag="VFR25_08255"
     CDS             22090..23487
                     /locus_tag="VFR25_08255"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET9941082.1"
                     /translation="MFARHRQLLQIGVFFLDALLIFAAWCVAYWLRFHALHLAAPRGV
                     PPIERYLWIGAVLTPFGLLILRSFRLYRSARTARLSQELWTLVQGVAILTAVAALASY
                     SIRGEVARSVLLIFAVVAALLLTAAHAAIRLALRAARRSGHNLRHVLLVGTGDLAERV
                     GRKMIAEADYGLHVIGMVSADATEVGNHVGPFLVVGCVADLPGLVTESGAELVYVAFP
                     RAEHEAEQDALERLGDSTAAVRLVPDLNRRHTLNASVEDFDGTPVVLVTETPEQGWNS
                     VLKRAFDLAVSAASLVLLSPLLVAIALWVKLDSSGPALYAQERVGSNGRRFRMLKFRT
                     MRTDAEAAGPQWSQAEDPRRTRAGEILRRLSLDELPQLWNVLVGHMSLVGPRPEQPVF
                     VDRFRASIPRYMLRHHVKSGITGWAQVNGLRGDTPLERRIEYDLYYIENWSMSFDMKI
                     LALTVVRIFRDPSAN"
     gene            23499..24104
                     /locus_tag="VFR25_08260"
     CDS             23499..24104
                     /locus_tag="VFR25_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9941083.1"
                     /translation="MALRLHVGCGTVYLDGYVNLDVEVPGYSFLATERPDLADRNRTT
                     VDRYYKKEESQATLEHGPGEIQYCVVDRFADLRALPFAPDSADEIRTVQVLEHLDMRE
                     APRVVRHWYEVLKPGGMLHVDVPDFEETARQLLAQPDEPSKDWYYRLVYGSQKNAYAF
                     HKNGFSPARLERLLREAGFREVRPVPNRIHFYPVAIAEAIK"
     gene            24133..25290
                     /locus_tag="VFR25_08265"
     CDS             24133..25290
                     /locus_tag="VFR25_08265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941084.1"
                     /translation="MRIFLVLHRSPNPAITSDLWKDNLHDPLVAMGHDVVLWGEGIAP
                     LFDLDPDDPGTAEPRARFTERFLEAVEEAHRASPLDLVITYVMDSHLEPAAIDRVRDK
                     IAPVLNFFCNNVHQFHLVQRNARHYSFCLVPEAEAILRYKRAGAEPLFFPMAANPDRY
                     RPMDASYRYDATFSGQRYGDRTSSMLALREGGVDAHAFGQYWDPAPVPEDSSAGFGQR
                     GILARAVELAFGGRNVLQAMRDRMDWDRLRTRHGAALHGPVSDEEYVALFSESRISLG
                     FLILGDTHRTSRPLRQIRLREFEAPMAGAFYVTGWLDEIALHYEIGKEIVCYRSRDEL
                     VDLSRYYLANDTERERIRRAGHERARRDHTWRRRFEDLFAELRRRKVIESV"
     gene            complement(25297..26727)
                     /locus_tag="VFR25_08270"
     CDS             complement(25297..26727)
                     /locus_tag="VFR25_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9941085.1"
                     /translation="MPLKPEPNRAPSPFEAPLSAMPPGARLWIERGARLAATAAYPAV
                     VALASVGRNKWVATHLDVSGIGVLAQIVQGQTWLGIAAGLGMSLPVAQAVSESLGRGD
                     VEGARRGFWTAFGLVLAAVLVIATAALLLAPALSRALLDSPSHADLVRWSVLGLVGYA
                     IANLLLGLFAGRSDVTAPVIYAAFGGAASLTATFLLVPRAGLVGATIGVTVFWPAGAL
                     GVLLVRGRAYRDVPGPPTRPIVSGEVAKRLLGIGTSALFLALLDLGTMLALRAHYVRA
                     HGVDANGLLQAALALSQIVGAVFYAYLASYAFGKISAASAAGGVDAARTYTRRQWKPL
                     LLLAAAAIAFTMVAASPLLHLLYSTRFDAARPLMAWALLGEFGRIAALTWSVGALPVG
                     GRRLWVGIGTVWPIALAVAYAAFVRAGAGAMSLPFAYAAGGGVLLLVAIWRMRRAGIG
                     PRAGDLAAAGAAAALLTGLAALITRA"
     gene            26796..27767
                     /locus_tag="VFR25_08275"
     CDS             26796..27767
                     /locus_tag="VFR25_08275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941086.1"
                     /translation="MNADPAAPLVSVLLASRNGARFLPEALASIASQTYPAIEPVLVD
                     DGSADGTRAILDEFAASHPRARVLRTDGVGLASAMTLAAREASGDYLARHDDDDRSHP
                     ERIARQMKYMEEHPDVGVLGTAAQVIDGEGRRIGTHPVPLTPMDIGRTLERASPFVHG
                     SVVMRRRAYEAAGGYRGAFRASQDYDLWLRMAPHAGLANLAEPLYEWRLHDAGVFSRA
                     RRDQLFYAAVARAFADERRDTGSDSVSLLSNGADPDGLLSRYPRAGRLALYLGEVHVR
                     EGLGSEGRRYLGRALRESEFGALPWWLLAWMIPWTKRGRRASRRART"
     gene            27722..28771
                     /locus_tag="VFR25_08280"
     CDS             27722..28771
                     /locus_tag="VFR25_08280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9941087.1"
                     /translation="MDQARAPGLAPRSHVKILLAADVDLSKPGGLETHVLEVSERLRA
                     RGHDIDLWARGVPDAVRALEPSRYDIVHRHTMAWPRSVEPGPRVVTTLHFCVAGKMDA
                     YVRLGRWKTLARPGNWTARADEGRAARAPGALIAVSKRVRDEFARFHGIDPARAAVIP
                     NGASFAPPREGREAWRRKHAIDPGTLVLLTIGRHDFVKGYDLLERAWREVRSRHSGAL
                     WVVVGGSGPERSEGRLVTGSVPRDHVTEWIHASDLGALPSYYEGCSVALLEMLAGGLM
                     TLAHDVGGALEAIRPGENGELVPRDMNAWVHAIDRAFRQSPPRRSGGLDPAYRWDAIV
                     DSIEEVYRRVRARYP"
     gene            28755..30002
                     /locus_tag="VFR25_08285"
     CDS             28755..30002
                     /locus_tag="VFR25_08285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9941088.1"
                     /translation="MPDIRDTLPPMRVVFLTSRLPFPPEGGDRFRVYHQIRTASEAGH
                     EVHLVTFAREHPDPLSIAPLERLLASITVVPLPPLLSAFRAAKAIAGHAPFQAAYYRS
                     RRMEDEVRRTLRRVKPDVVVTHLFRMAPYALANQGTCQARWILDLTDVISVGIARSLP
                     FRKGPDRWLYREERRRIRRYESEISPQFDECWVISDAERNELTRIAPGADVRVVPNGL
                     VPGSRLDGNAPDKARLLFLGFHQVFHNRDALRFLVQEVLPRVRASVPDATLDVIGAGA
                     EALGPWARGPGVHVLGHVPRLEPYFVRATVFVAPHRFAAGVQNKVVHALANGVPVVST
                     SVVRRGLEPIPRGLILTGETADEIAARVVDVIHHPARAAWMAEQGREWALATFSWHAT
                     LEALEAHDTDSAADAEPLVSSAV"
     gene            29999..31012
                     /locus_tag="VFR25_08290"
     CDS             29999..31012
                     /locus_tag="VFR25_08290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET9941089.1"
                     /translation="MIGPPPNLVFAPLAFGCALALTPLLGRIAEGTGYLDHPDLRKHH
                     AQPTPLLGGVAIGLSIVSALLLANYVFERTVNVPAPGIMLGAAISLVLGLIDDWRPMG
                     PLGKLVGQVAAAASLVLWGPRMEWLHGNPIVVATAIVAVVALLNAVNFLDAMDGIVGT
                     VLPIIGAGFVALAVLYQAPLATALGWSIVGACAGFLVYNAPPARIFLGDAGSHLLGFG
                     LATLALQALRDEPTLPHVASVTILVAYPLFDVMFVMWDRSARRKPIYVGGVDHTTHRL
                     SRRWGAWPTLGLLALLNVIHVALALWVWGLSGPQAVIPLVLIFGLGYAIFGAYLRRLS
                     PTP"
     gene            31175..32797
                     /locus_tag="VFR25_08295"
     CDS             31175..32797
                     /locus_tag="VFR25_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9941090.1"
                     /translation="MDPLERLSRGLTQWERELLAQPRSPARPRASTDLERVNNVLQAS
                     RQVTGTLDWNELLIRVVDAVVQIAGADRGFLMRMREDGKLHFEIARSRDEATLPPDEF
                     QISWGIAEEAAHRRETVWVPDAVGSSLFQDRKSVRELSLRTVVALPILTAGRVLGVLY
                     VDSHSIAHEFTPEDIAILEGFAAQVAVALENARLHEELKDSKNRLEMENLNLRRALKE
                     ESRYGLLVGRSPKMLRVIELMEKVIPTQVSILIQGETGTGKELIARAIHLNGPRREKN
                     FMAVNCGALPESLLESELFGYRKGAFTGASEDRIGLFEAADGGTLFLDEVGEMPATLQ
                     VKLLRVLQDSQIRRVGDTVSRRVDFRLIAATNRDLKTEVEAGRFRQDLFYRLNVVPIS
                     LPPLRERGEDVLLLSQHFLELFSKQQEKPVRGLSAESRELLLRHPWPGNVRELENAMA
                     RAVALADEGGAIEPLLFGLGAPVTRRWDGQHTLRETLDAVEAETIREALRQCESNVSR
                     AARALGVSRQHLHNRMNAHGIRRSRTLSESAG"
     gene            32803..33837
                     /locus_tag="VFR25_08300"
     CDS             32803..33837
                     /locus_tag="VFR25_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9941091.1"
                     /translation="MSSTIAPRATSGKDAIASRRLSSLRAILMQSDAEIRAQFPEYFL
                     TYYTYLRIIRNPARIEHLFQKGYDMYRYLEAEGKDVLDLGAGFGIEALLVAIYGARRV
                     VATEIDHDMVAVGTYLAEKIDPPATHFESKYGDGIGMEFPSASFDGVMANCVISHVRD
                     LEGFLAEANRLLRPGGIFFLSDENNSLYLPARGRRRRGWHSMEHEPDGPYFAARRAMI
                     GERFPGLSSNQLLEAVRQTRGLWGEGVEAGVRELVETGRVERKPEFLYRDPRDGQYPE
                     RELNPFWMMRKFKDAGLEPELLPAFFSRAIEVHPRQWVKDFLRFFCVRWPALSVYVWP
                     IMRLRGRKLR"
     gene            33872..35062
                     /locus_tag="VFR25_08305"
     CDS             33872..35062
                     /locus_tag="VFR25_08305"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET9941092.1"
                     /translation="MKSVSVTLIGLFLAANAFAAPIDAPATPPPDLPIPQNLVAKDKP
                     GDSGGSVVLTWTDPVSLPAGADIEVLRAEPPAYDLQPIDHVAAGKGTYTDTSAKDGVA
                     YRYGLRTSLAATDSTGAVSVAAATGAPVVSEPVSSHDNWFKVERTNSFIASILFVIII
                     LGSIQVARSGKEIFIRRIAGLNAIDEAIGRATEIGKKVMYIPGIQSMDDIQTVASVAI
                     LGHVARSTARYGTDLEVPNKDPLTFASAREVVRAAYLEEGRPDLFREEMVNYVTYDQF
                     AFTAAVSAKMTREKPAAIFLVGYFFAESLILAETGQSTGAIQIAGQADPTQLPFFVAT
                     CDYTLIGEELYAASAYLSREPVLLGSIRGQDIAKGILILIAIAGIVLASVHLVDISPW
                     IRTK"
     gene            35059..35859
                     /locus_tag="VFR25_08310"
     CDS             35059..35859
                     /locus_tag="VFR25_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941093.1"
                     /translation="MSFEFGQVALVLCFAIVPIIGIVLGLNAPGLRSRGPILIGVVAG
                     LVPILAMYIPKGFVSGPEGMGPRLDNWLIILAGFALLLGIVNVLQNNFKKIARRESGW
                     FFSVVLLIGLFVTGGFGVYGALSRVGIGFNPDGSPTPFKYMTDWFFQPLQSTIFALLA
                     FFMASAAFRAFRARNVEATILLIAAGLVMLGRVPILEFLAVPFPFLHAAAASGSQGLG
                     KLTEWIMDVPNGAAQSGIIIGAALGAASMAIRVILGIERGYLGLGKGE"
     gene            35873..36733
                     /locus_tag="VFR25_08315"
     CDS             35873..36733
                     /locus_tag="VFR25_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941094.1"
                     /translation="MERLLRLDRRWIFLFVLLSVAIPIVIPLGLPVTTTESTRKAFEF
                     IEAMKPGDTVLISFDYGPSSAPENDPMAEAVMRQCAIKKLRFIVIALYPLGGLNLANN
                     VVAKVAAEYPDLKYGVDYVNLGYKDGASAVMRRMGDDIAGAFPTDEAGTPIAQIPMMK
                     GIGSLRQVQLVFTVATGIIGEWWITQVHPQVGTPVIIGPTAVSAPKYYAFLNSGQLVG
                     MIGGMKGAAEYEKLMRAAHPDMERFYKSTRDFTATKGMDGQTVMHTVVLLFILLGNLA
                     FFLTQKRAVR"
     gene            36730..37356
                     /locus_tag="VFR25_08320"
     CDS             36730..37356
                     /locus_tag="VFR25_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941095.1"
                     /translation="MSHLPPIGVWIAAFLTLSALSFLYKDNPFFRFAESLFAGVSLGY
                     YVGITLDQTLVPNLIEPMQQDFGKHWDLLVPGLLGVLLYARYLPRIGWVSRYALAIYV
                     TYYIGLDFTRRLHGEAMPQIARVIVALPGPNPLAFIFVVGVLSVLVYFFFSKEQGPVT
                     RRISRLGIWFLMISFGAAFGFTVMGRVALLIGRLNFLILDWIYPILGI"
     gene            complement(37310..38083)
                     /locus_tag="VFR25_08325"
     CDS             complement(37310..38083)
                     /locus_tag="VFR25_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9941096.1"
                     /translation="MAQHLLLVDDEAHNRKLLRMVLRQGDYVFFEADNGNQALEILGQ
                     ETIGLVLLDLMMPSPNGFDVLLAMKKDDRWKEIPVIVASASTSPEDVERSLTLGASDY
                     FMKPLTEWDVRFQLPIKVRNALAAHRATEDRLRAERMKAVSAMAVALNHEINNPLQVI
                     QGNAQLLYVQPTLTPEAREKVARIRAATETIAGLTQRIAALRDVVTVDYPAGNRTTVP
                     MVNFKASSDAPDPGPAVKPATDPEIKSRESGRSSPESGS"
     gene            complement(38058..38480)
                     /locus_tag="VFR25_08330"
     CDS             complement(38058..38480)
                     /locus_tag="VFR25_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9941097.1"
                     /translation="MRILIIEDDPMNAKLFGLILTRKARYEVEVTEDPAYVADRVRQG
                     LVDVVILDVSLSNSAWDGVPVDGLEICRRLKRDPETRRTPILLATAHAMKGSRERFLA
                     ESGADDYIAKPIVSADELIARIRTLAEKGAHGPAPASR"
     gene            complement(38468..40408)
                     /locus_tag="VFR25_08335"
     CDS             complement(38468..40408)
                     /locus_tag="VFR25_08335"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941098.1"
                     /translation="MPSTYNPSLAAPGVSSPAGIARAKRRSLVFAILGALLACLTVGW
                     IASSSSVPWAAWPAGLALILAAGWLHRTRVQDLDRAQAREAQLSLYRVGDTLARFDRE
                     DDLVHHALDAVAEGTGLRHWALYAHRGGRGEFTLVATRGLTPEANVALEPDAPGPEAR
                     AHASRAAWLGEIVVGDLAGSERDAFAATLPELGADTAVVSVPFLEHEGLPATLQCFLP
                     AGASLANEERALLRWMAAQLASGLRRLRLERRDQILASYLVGTSEIVLELDLDGVVSS
                     ANRAAELALSAQPGELLGRRLHDLAFAPGATGADGEALVPASGPAAALDVARASGEFS
                     GELGFRRGDGSTFPAEVRLSPAHDRAGALTAIVLVARDITERREGEQEDRARGAELGS
                     MNERLLDANHELELARRLQNDFLANTSHELRTPLNAVIGFATLLEQRIDADEGERLDF
                     ARSIRQAAEHLLSVINDLLDLAKAEAGRFELRLLVADIAPTIRGAVDAVQPMASAKGL
                     AVRVDVPKEPLSAAVDLARFRQVLLNLLGNAVKFTDRGEVRVRAGIDAGSIVVSVEDT
                     GVGIAPERRAALFSKYAQADPSYHRRQRGTGLGLAISRALVDLMGGTIQLTSEGVNRG
                     TVVRITLPVAQAAPTEAESCGS"
     gene            40554..>41091
                     /locus_tag="VFR25_08340"
     CDS             40554..>41091
                     /locus_tag="VFR25_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941099.1"
                     /translation="MRSRPILVSLLLAIWSLALSGCSNNSDRVTGNERLIRGAGGLGS
                     TLVETTIPDRDTYVTPGTANYGSVLLVGRDATFEARAFFKILRINLPSTDFVSVVPTG
                     NVLLDFPHVPLRNEPLSIDLELRETATALADSGTIAWPGPAPGVLLASSTYDFSGDLV
                     MDLGPGSYSRVVQWTTPDP"
BASE COUNT         6031 a        14368 c        14064 g         6628 t
ORIGIN      
        1 gaggcgctcc agttctacca gtcctggcgc gtgttcgcgg cgcaggaggg gcgcgaaggc
       61 gacatgctcc tccgcatcta cgacgtgacg gcgctcatgg ggagaaccca ggatgcgctc
      121 aagggctaca acgacctcgt gaacaagttt ccgcgcacgg ccgtcgcgta cgaggcccag
      181 ttccagatcg gctacctgta cgagaccgcg gtccaggatc tcgacgccgc gggccgcgag
      241 tacgacaagc tgaagagcca gccgacgtcg gagttccagc agcaggccat gcgccgctcg
      301 caaagcctgg ccgcgatccg cacgtaccgg aactcgctcg cgtccgactc gaccggagcg
      361 cgcgcccacg ccgccttcat gatggcggag ctcaactact tccagctggg gaagccggat
      421 tcggcaatcg tccagtacca gcgcgtcgag atggagtttc cgcacagcgt ttacgcggcc
      481 aaggcggcgt acgcccgcct ctggatcgcg acccaggaca aaggggacac gctggaagcg
      541 atgcgcatcg cggacatgat ggggcaacgc taccgcggaa cgccgtacgc cgaatcgggc
      601 ctctatctct ggaagcagtg gagcgggcgc accgacgagc ggaccgcgct cctcgattcg
      661 ctcctcgcga atccggacac gtcccaggtc gtcgtgctgg agccagagct cgccgattcg
      721 acgttcgcgt ctcccgattc cacgctcatc cgaccccaga cctacgccat gacgccggcg
      781 gacagcgcgc gcatggactc gctccgccag cacgccctcg agatccgcga gcagtacatg
      841 cgcgagcacg gcgacaagaa gcgccccaag cctcagcagc aggtgcggcc ccaggggccc
      901 acgccgcagg tccagacgcc gacgccctcc gcgccgaatg ccgccggcgg cgcggcagcg
      961 gctggcgcgg ccgcggcggg agccgccgcc gccggaggct cggcccccgc ggacagctcc
     1021 acgaacccaa cgccgccgcc gtccaccacc gcggctcccg attcgacagc acgccccgac
     1081 agcacgaccc atgaggacga cacgggtcca tgaacctggt ctcctgctcg gtcgtctcca
     1141 atcacgagct caaccccggc aacttcctca tcaccctcaa ggtgccgcgg ggcttcgcgc
     1201 gccctcagcc ggggcaattc gtgcacctgc gggtggggga cggcctggag ccgctccttc
     1261 gcaggccgta cagcctcgag ggcttcatgg agaagggcgc gcttcgcgcg gtccggatct
     1321 actactccgt cgtggggcgc ggatccggcg tcctggcccg gcaggagaag gggacgaagc
     1381 tcgacttgat cggcccgctc ggcatcggct tcgcgccgag accgcgccgg ctgccgatcc
     1441 tcgtggccgg cgggcgcggc gtggcgccgc tcctcttcct gagccggagg cttcgggagc
     1501 ggaagcgtcc gtacgtcttc ctgtacggcg cgcggtcgaa gacggagctc tacggcgtgc
     1561 gcgaggtccg cggcggccgg gtgcacttgg cgacggacga cggctcgatc ggatacaagg
     1621 ggagcgtgat ggatctcctc gcgcgcgagt ggagcgagag cgggcacacg ccgctcacgg
     1681 ccgagatctt cacgtgcggc ccgcacggac tccttcacgc gatctccgat ttcgcgcgcg
     1741 accggggcat ccggtgcgag gcctcgctcg agggaccgat ggcgtgcgcc gtgggcgcct
     1801 gccgcggatg cccggtgcct ctcctcccgg gggtggactc cggacgctat cccgccatgt
     1861 gcctcgaggg gcccgtcatg gatgccaccg tcgtcgactg ggcgaggctc ccatgaagcc
     1921 gtcgctcgaa gtccggatcg gcgggctcac gctccggaat cccgtgctcg tcgcgtccgg
     1981 catcatcggc tacgggcgcg agtacgagcg gctcaccgat ctctcgcgca tcggcggcat
     2041 cgtgacgaag acggtgacgc tccgtccgcg cgccggcaat ccgccgccgc gcgtgtgcga
     2101 gacgccgtcc ggcatgctca actccatcgg gatcgagaac cccgggctct cgggctacct
     2161 cgagcagaag gtcccgatcc tccgcgagct cccctgcgcc gccatcgtga gcgtcgaagg
     2221 ggaggacgtg ggcgagttct gcgagctcgt gcggggcgtc gacggaagcg gcgcggcgca
     2281 cgcgatcgag gtgaacatct cctgccccaa cgtgggcccg cacggaatgc gctactcgac
     2341 cgatgccggc atggcgcgcg aggtgatgag cgcgatccgt ccgctcacga agcttccgct
     2401 catcgccaag ctcaccccca acgtcacgcg catcgccgag atcgcgcagg cgtgcgagga
     2461 ctgcggcgcg gacgcggtgt cgctggtgaa cacgttcgtc gggatggccg tcgacgcgcg
     2521 cacgcgaaag ccgatcctcg gcacggtgct cggcggactc tccggacccg ccatcaagcc
     2581 gctcgccctg gccaagctct gggagacggt ccaggcggtc gacatcccgg tggtcgggat
     2641 gggcgggatc gcccgcccga ccgacgcggt ggaattcctg ctgacaggcg ccacggccgt
     2701 ggaggtaggc acggcgatgt tcgcgaaccc gagcctcccg gaggagtgcg tggatgcgat
     2761 cgaggaggcg gtgcgcctgg ccgcgggcac gagcgtcgac gatctgatcg gcgcgctcga
     2821 ggtcccggaa ggacgcgggg tgatccgggc cggacgcggg gcgcggcgcg agccggggag
     2881 gcacgcgtcg tgagcttgcg cgatcggctg atcgtcgcac tcgacatgcc cgatctggag
     2941 cacgcggtcg cgcacgtgga ccggctcggc gcgggcgttc tctggtacaa ggtcgggctc
     3001 cagctcttct acgcttccgg gcgccgcgcg gtcgagacga tcgccgggcg cggcaagaac
     3061 gtcttcctcg atctcaagtt ccacgacatc ccggccacgg tggagaaggc gatccgcgcg
     3121 ctcgatggcc tcccgatctc gctcctcacc gtgcacgccg cgggcggccc cgcgatgctc
     3181 gaggctgccg ctcgtgcggc gcgctcgatc ggctcccagc cgaccgtgct cggcgtgacc
     3241 gtgctcacga gcctggacgg gaacgagctt ccgggcgtct gggatccagg caccgatctc
     3301 gaggcgaagg tgctcgcgtt cgcgaagctc tgcgcggacg ccggcgtcgg aggcgtggtc
     3361 gcgtctccac gcgagctcgc agcgcttcga cgcgcgcatc cggctccgtt ccggatcgtg
     3421 acgcccggca tccgcggtcc cgaggatcga tcccacgatc agaagcggac tctttcgatc
     3481 ggcgaagcct tcgcgctcgg cgccgatcac atcgtcgtcg gccgcccgat cctcgaagcg
     3541 gccgatccca aggcggtcct cgcggcctac gaagcggcgg cgggcctcca ttcctccacc
     3601 gaaaggatga tcggatgaag gtcctggtga ccgggatcac cggtttcgca ggcagccacc
     3661 tggtcgacta cctgctcacg atccccggcg tcgagatctc cggcatcctc cgctggcgaa
     3721 gccgcaccga gaacatcgag cacttcatgg acaaggtgcg gctggtcgag tgcgaccttc
     3781 gcgacgcgtc ctcggtgcgc gacgcgatcg acgagctccg cccggaccgc atcttccatc
     3841 tggcggcgca gagcttcgtg cccacgtcgt ggacggcgcc gaccgaatcg ctcgtcacca
     3901 acgtcatcgg ccagctcaac ctcttcgagg cggtgaagaa gctcggcatc aagccgcgga
     3961 tccagctggc ctgctcgagc gagcagtacg ggatggtcta cgaagacgag cttcccatcc
     4021 gcgagaccaa tccgctccgg ccgctctcgc cctacgcggt gagcaaggtg gggcaggacg
     4081 ctctcggcta ccagtactgg atgagcttcg ggattccgat cgtgcgcacg cgcggcttca
     4141 accacgaagg cccgcgccgc ggaccggtct tcgtctgctc ggatttcgcg cggcaggtcg
     4201 ccgacatcga gaagggacgg aaggcgccgg tgatccgcgt ggggaatctc gacgcccgcc
     4261 gcgacttcac cgacgtgcgc gacatcgtgc gcggatactg gctctccctc gagaaggccg
     4321 agatgggcga ggtctacaat ctctgctccg gcaaggactg gacgatccgc gcgatgctcg
     4381 atctccttct ctcgatgtcc aacgtgaagg tgaaggtgga gcaggatccg gcgcgcatgc
     4441 gcccgagcga cgtgccggtg ctcctcggcg atgcctccaa gtttcacaag gcgaccggat
     4501 ggaagccgga gatccccttc gagcagacgc tccgggacct gctcgagtac tggcgggccc
     4561 ggtagccgtt gcgtctcttc atcacggggg tggccggttt cgcgggcggc catctgctcg
     4621 agcatctcgc ggaagtaggt ggcttcgaag tccacggagc ggatcacgct ccgctcgagg
     4681 cgcacccgaa cgcggccgcg ctccgcgcgc ggctcgcctc ctatggcgcg ctcgacatca
     4741 cgagcgccga tgcggtggat tcctggctca ccccaaggaa gcctgacgcc ctgatccatc
     4801 tcgcggccca ggcttcgggc gcggacgcgc tcgagaagcc ggtcgcgacc tacgacgtca
     4861 atgtcatggg cacgctccac gtcctggaag ccctccgccg cacggcgccc tccgcgcgcg
     4921 tgctcgtgac gggctcggcc gacgtctatg gctcgggcgc ggcgggcggg cgcatccgcg
     4981 aggacgcccc gcttcggccg gccaacccgt acgccgggag caaggccgcg caggacgtga
     5041 tcgccgagat ctacgctgcg accttccacc ttccggtcat ccggacgcgc acgttctcgc
     5101 acacggggcc cgggcagagg cctcgcttcg cgctcgcggg tttcgcggat cagctcgccc
     5161 ggatcgacgc cgggttcgca ccgcccgaga tccgtgtcgg caatctcgat gtcacgcggg
     5221 agtatgggga cgtccgggac gtggtccggg cctatgtcct cctcctccag cgcggcgagc
     5281 cgggcgcggc ctataatgtc gccacgggcc agggccacaa gctccgggac cttctcgacc
     5341 gcttgatcga gatctccgga gtcgcggcca cggtcgtcgt cgatccgtcg cgggtgcgat
     5401 cccgcgacgc ggaccatctc gtgggagatc cggccaagct cgtgagcgcg accggctgga
     5461 agcctcttcg ttccatcgaa cagacactgt ccgatctcta ccgggacgcg cgcgatcgcg
     5521 tgctctccca agcgaaggcc taggagccgc cccttgcgga tcacgatcgt gggaactggc
     5581 tacgtcgggc tcgtcaccgg agccgggttc gccgatttcg gcaaccaggt gctctgcgtc
     5641 gacgtggacc gcgcgaagat cgagacgctg gagcgcggcc agctcccgtt ctatgagccc
     5701 ggcctggacg acctgatcgc gcggaacgtg cgcgagcggc ggcttcgatt cgggctctcg
     5761 ctcgaggagg cgacccgctg gggcgaggcg attttcatct gcgtcgggac gccccagggg
     5821 gaagacgggc gcgccgacct caagtacgtc gacgcggccg cgcgcgtcat cgggcgggcc
     5881 atgaacggct accggctcat cgtccagaag agcacggtcc cggtcggcac cgcggagcgc
     5941 gtgcgcggga tcgtgaagaa gaacgcgaaa cgcggaatga agttcgacgt cgcctccaac
     6001 cccgagttcc tccgcgaggg gaccgccgtc gagaactgga tgcgccccga ccgcgtggtc
     6061 atcggagccg attccacccg ggcgcggcag ctcctccgcg agatctactc gcccctctat
     6121 ctcatcgaga cgccgatggt gctcaccgat gtgcgcaccg cggagctcat caagtacgcg
     6181 gccaacgcgt tcctcgcgac caagatctcg ttcatcaacg agatggcgaa cctgtcggag
     6241 gcgctcggtg ccgacgtgaa ggacgtggcc aaggcgatgg ggctggaccg ccgcatcggg
     6301 cccaagttcc tccatccggg gcctggattc ggaggctcgt gcctgccgaa ggacacgcgc
     6361 gccatgaccg gattcgcgaa gaaagcgggc gtgaagctcc ggatcgtcca ggccgcggtc
     6421 gagaccaacg acgcgcagca ggacatcgcc gtcgggaagg tgatggacgc gctcccggga
     6481 gcccggaagt cgcgcaccgc cgccatcctg ggcctctcgt acaagcccga cacggacgac
     6541 ctgcgcgagg cgccagcgct ccacatcatc cgcgcgctct tgaagcgcgg ggtgcgcgtg
     6601 cgcgcgtacg atccggtcgt gcgcgaaggg gccgcgggcg tcccggcggg gatcaccttc
     6661 gcggagaacg cgtacgacgc ggccaaggga gcggacgcgc tggtcatcgt cacggagtgg
     6721 aacgaattcc gaaggctcga cctcgcgagg atcaagaggg ccatgcggcg gcgggcgatc
     6781 gtcgatctgc gcaacatcta cgacccggct tcggtccgga agctcgggtt cgactacacc
     6841 tcggtgggga gaccaaatga agctgattga cggagtggtg gtgaagccgc tcaaggtgat
     6901 cgccgacgag cgcgggtact tgatggagat gatgcggaag gacgacccct tcttccagcg
     6961 gttcggccag acgtacgtct cggtcgcgta tccgggcgtt gtgaaggggt ggcactatca
     7021 caagatccag accgatcact tcgtgatcgt gaaggggatg atgaaggtcg tgatgtacga
     7081 cgaccgcgag aagtcgccga ccaagggggt cgtgaacgaa ttcttcatgg gcgagcgcaa
     7141 tccgatcctc gtgacgatcc cgcccggggt gtggcacggc atgaagggaa tcggcagcga
     7201 gcccgccatg ctcgtcaact gcccgaccga gccctacaac tacaaggagc cggacgagta
     7261 tcgctgcgac ccgcacaaga acgacatccc ctacgactgg gcgcagaagg atggctgagc
     7321 gcctggatct caaccggtcg cgcctcctcg tcacgggcgg cgctggcttc atcggcagca
     7381 acttcgtcca ccatctccgc cagtcgtacc cccaggcggc gatcacggtg ctggacaagc
     7441 tcacctacgc cgggaacctc gccaatctcg aggtggtgaa ggacgacccc ggctaccgct
     7501 tcgtgaaggg ggacatctgc gaccggaagg tcgtggccga ggcgatggag gggtgcgact
     7561 tcgtcgtgaa cttcgccgca gagacgcatg tcgaccgctc gatcgatcac gcggaggact
     7621 tcgtcctcac ggacacgtac ggcgtgtggg tgctcctcga ggaggcgcgc aagcgcaagc
     7681 tcacgcggtt cctccaggtc tcgaccgacg aggtctacgg cgagatcctg gacggcgagg
     7741 ccaccgagga atcgccgctc ctcgcgcgga atccctacgc cgcgtccaag atcggcggcg
     7801 accggctcgc gtattcctac ttcgcgacct tccagacgcc gacgatcgtg acgcgctgca
     7861 gcaacaacta cggcccctac cagtatcccg agaagctgat cccgctcttc gtgaccaacg
     7921 cgctcgagga caagacgctt ccggtctacg gaagcggaag gaacacgcgc gactggattc
     7981 acgtcctcga tcactgccgc gcgatcaccg ccatcctcga gtctccgggc gtggaagggc
     8041 acacgttcaa catcgccggg aacaacgagc gctcggtgaa ccagatcgcg gatctcatct
     8101 tgaagcggct cggaaagccg gagtcgctct tgaagagcgt gaccgaccgg ccaggacatg
     8161 atcggcgcta cgcgatcgac gcgtcgaagc tcgagcggat gaccggcttc aagccgcagg
     8221 tggagttcgc gaccggaatc accgagacga tcgactggta caagacccat cgctcctggt
     8281 gggaggcgat ccgctccggc gagttccgcc attactacga gaagatgtac ggcgcccgct
     8341 aagggaatgt agggagggaa gcccccctgc gccgcctcgt ccgctccacg gcgatcctgg
     8401 gcttcggctc gatcgccacg atcgtggccg ccatcctgcg cgcgaagatc ctcgccgcgt
     8461 ggctgggacc ggaaggcacg ggagcgctcg cgcagctcgc cgcgttcacc gcggtcctgg
     8521 tcccgttcgc caccctgggg gcgggaagcg gcgtgatcac gatgatcgcg gacgcccggg
     8581 cgcgcggcga cctggaccgc gtgcggcgca tcacgagcac cgcgcggtcg ctgacgtggg
     8641 ctgccgggat cgcgctcgcc gtgctgtcct tagcgctctc cccctggatc accgacgcgg
     8701 tctaccgcga ccgcgcgttc gtctgggcca tcctgatcgg cgcggccacc atcccgctct
     8761 ccggcgtcgc ctcgatccag atctcgatgc tccagggata cggcgccgtc cgcgcgatgg
     8821 cgattctgaa cggcctcgtc gcggcgacga cgatcgccac gatcgtgccg atggcctggt
     8881 tcttcggcgt gaagggagcg gtgacgcagc tcatcgtcct cgccgtggcc caggtcctgc
     8941 tcgggaaccg gctcctcaag ccgtacgtgc cttccgctcc cgccgcggca gagtcgaccg
     9001 tggatcgctc actcctgcgc cccatcgcgc gcttcggcgg gagtgcgctc ctcgtggggc
     9061 tctcctcgac gctcacggtc ctcatcctgc gctccatcat cgtggacaag ctcgggctcg
     9121 ggcagaacgg gatctatcag gtatgcgtcg gcgtgtcggg cctcacgatg cccctgatcc
     9181 tgaactcgat caccgcgacc gtctggcccg agatcgccgc acagccgacc gacgaggggg
     9241 cgggcgttcc gatgcggagc ggcatccgtc tctgcttcct cctcaccacg gggctcgccg
     9301 cggcgctcat ggtgggagcg ccggtctggg ttccgctcct ctactcgacc aagttccgtc
     9361 ccgcgctcga gctcctgccg tatcagttcc tcggcgatta cttccgcgcc gcggcctgga
     9421 tgttcggcat ctggctcgtg ccccggaatc ggctccgccc gtgggtgctc ttcgacgtcg
     9481 tgtacggcct cgtgatcctg ggcgccttcc tcttcctcgt ggatcggatc ggcgtgcgcg
     9541 cgaccgtgat cgcgtacatg ctcgcccacg tcagccacgc ggtgctccac tacggcctgg
     9601 cgcgtcgcgc gatcggattc cggctcggcc ccgacaaccg cgtgctgctc ctcgcgtcgc
     9661 tcgcgctcct gctcctcctc ctggtggtgc gccccgagga cacccccggg atggtgatcg
     9721 gagcgatcct gacggccgcc tgggccgcgc tcgtggtgcg cccgcgcgag tggcggggcc
     9781 tccgcgacat ggcgctgcgg cagatccggc gcttcgcgcc aggtccgggg cgcccgggag
     9841 gggaatgagg ccatccgtcc taggggctat ccgccgtccc ccaggcatgg catcatcgtc
     9901 gcccttgacc cagattcgct cgatcagcca tcggatcgcc ccccgcttcg tcctggggct
     9961 caccgcctgc tgcgccggcc tcctcgtcct gaccacagcc tgggcccaga cgcccggagc
    10021 gggcgtgtcc gatgatgccg cccgcgccgc gcagatcgac actcccatcg agggacgcgt
    10081 ggatcccacg acctaccgca tcggtcccgg ggacgagctg gccctccgct actcggacct
    10141 cctcgagccg aagatcctgc gcgtcgctcc ctcgggcgag ctcctcctgc ccgacgtggg
    10201 aacgttccac gtcgccggtc tcacgctggc ggagacccag gcgcggctca aggaggcgct
    10261 caagccgtac atcaagggga aggggttcgt gctcgcgctc agcaggccgc gccggttccg
    10321 catgccggtg ctcggcgacg tcgagcgccc cggctcggtg acgctccagg ccccggtgcg
    10381 cgcgtccgaa gcgatcgcgg cggccggagg agtcaccgcc accggagcga ggcgcggcat
    10441 cgaagtgcgc cgctcgagcg acacgctccg cgtggatctc gtccagtaca cacgcggcgg
    10501 agacctcgcg tcgaacccgc tcgtcttcga gacggacgtg atctacgtgc cccccgcgcg
    10561 gaagcgcatc gaggttctcg gcgcggtgcc gcacccgggg gcatatgatt tcgtccccgg
    10621 cgaccgcctc tcgaccgcga tcgcgctcgg cggcggcacc ctcgcggacg cggcgcccga
    10681 agccgccgag ctcgcgcgcg tgcgcgccga cggaacccgg agcgtcgaga agatcgcgat
    10741 cgcccaggcg ctcgaggcgc caggcgggga cgccgatccg gtgctcgcgg aaggggatcg
    10801 cgtcttcatt ccgtcacggt cgcactacct gaagggcgcg cacgtcgagg tcgaaggcga
    10861 agtggcgcgg cccggaccgt acccgatcac ggagggcgtg gaccggatcc gctccgtgct
    10921 cgagcgggcc ggcgggttca ccgaccaggc ggaccgtgcc gcgatgcgcg tcgagcgcat
    10981 ggtgaaggac gaggagcccg attcggtctt cctccggatc gcgctccagc aggaggagct
    11041 cctcgccggc tccgaccggt cgtacgtgat cgcgaaatcg cgcgagcggc aggccatctc
    11101 cgcgcacgtc ggcgtgctcc tcgaagccgg cgacgagcgg ggcgatctcc cgcttcgcga
    11161 cctggaccgg atcgtggtcc cgaagcgagt cccctacgtc tcggtccagg gcgaggtgag
    11221 ggcgccgggc tacgtgccgt atcgcgaggg atggaaggtg gacgactacg tgaaggcggc
    11281 gggcggcacc acgtcgcgcg cctacaagag ccggacgcgc gtgaccttgt cgctgaccgg
    11341 ccgccccgtg gccgcggagg acgtcggggc gctccgccct ggggaccagg tcctgatccc
    11401 gtccaagccc gaccgcaatc cgtggggcac gatccgcgac gtgatcggcg tggtcgccgc
    11461 cgcgtcggcg atcgtgatcg ccgtggaggc ggtgaaccag tgagcatggc gctcgcgcgg
    11521 cgggaccggg aagacttcat gggcgtcgtg cgcgcgcgtc gccgcttcct cgcgctcaac
    11581 gtcctcgcga ccacgctcgt cgcgctcggg atcagcctga tcctcccgaa gtggtacacg
    11641 ggtcgcgcgg tcctccttcc gccggtggag gacgacgcca cctccgcgct cagcatgctg
    11701 atgccgaagt cgctcggctc cgtgcgcctt cccggcgctc ccacgatggc ggacgtgttc
    11761 gtcgccgtgc tgaagagccg ctccgtggcc gaccgcctgg tgtcgcgatt cgatctcgtg
    11821 cggcgctatc acctgaagga cgaggagcgg gcggtgaagg agctcagctc gcacgtggcc
    11881 ttccgcctcg gcgacgaggg aaccatcacg atcgccgtgg aggacaagga ccccaagacc
    11941 gccgcggcca tggcgaacgc gtacgtcgag gagctggacc gcttcaacgt cgagacgcga
    12001 acctcgagcg ccaagcgcac gcgcgccttc atcgacgagc gcctgaccct ggccaagcag
    12061 gacctcgcga acgccgagga ccggatgcgc gcgtatcagc agcagcacaa gctcccggcg
    12121 ctatcgcctt ccgcccaggg cgacaccgac gtcggcgcga atctcatggc gcagaagatc
    12181 gcgctcgagg tgaagctcca ggtgctccag caatcgctcg ccgagtcgag cgaggaggtg
    12241 cgccgagtcc gccaggagct ggcggcgatc gacaggcagg tcggcgggct cccccaggcg
    12301 gggatcgaga tcatgcggct ctggcgcgac gtgaaggtcc aggagcaggt cttcgagctg
    12361 ctgacggcgc agctcgagga ggcgcgcatc cgggagaccc gcgacacgcc gacggtgcag
    12421 gtgctggatc acgccgtggt cccgctccac aagtcgcggc cgaagcgcgc cctggtcgtg
    12481 ctcgcggggt tcgtcatcgg tctggccggt agtctcacat gggtgcttct gcacgaccgg
    12541 ccgcgctagc ggcgcctccg tcccgcctct ccctcggggc tctggcctgg atcgcgctgg
    12601 ccgagctcct tctcctcctc ctcggttgga agctcggcag cctcgcctgg ccgctggcgg
    12661 gcgccttcgc cgtcgcgttc gcgctcgtgg cctatcgatc gcccgagacg gcctggatcc
    12721 tggtctggat cgccacgccg ttctcacgcg agatcgtcgt ggtgggcggc gcagcggtct
    12781 ccgttcccac cgagcccatg atggccgtgg ccgtcggcgt ctggatccta cggcgctggc
    12841 cctggcgcga gggctccgcc ggcccgcgat ccatcacccg tccgctcttc gtgctcgccg
    12901 cgatcgccct cgtttcgatc gccgtgagcc ggtttccctt cgagggaatg aaggcgtgga
    12961 tgatggcggc gctctatgtc gtgttcggct acctctactt cgtctcgacc ccgttcgatg
    13021 cccgccgggc gaagctctgg atggcgctcg gggtgctgct cgcctccctc ctctcgatct
    13081 acgcttcgta tcgcgtcctc tcggccggca tcggagtgcg ctccgcgtac ggcgcggcgc
    13141 gcccgttctt tccggagcac ggaacgttct ccgcctacct cgccttcttc ctccccgtcg
    13201 ccatcgtgca gagtctcgcg cgcgagcggg gacgacttct ctggaccctc ggctcggcag
    13261 cgatcctcgt cgggatcgtg ctctcgttca cgcgcgcggc gtggctctcc gccgtcttcg
    13321 tcctcccgct cctgctcgtg tcctggatgc tggggcgagg cggcgcttcg cgcctgtggc
    13381 ttccgctcac gatcgtcgcg ctcgtgtcgg cctggatcgg gacgtcgcgc gtggcggatc
    13441 cgtttctcgg gcacgcgcgc accatcgtga acagcggcaa cgtctccaat ctcgagcgcg
    13501 tcaatcgatg gatggcggcg gccgagatga tccgcgcgcg accgctgacc ggggtgggat
    13561 atggggtcta ccctgaggcc taccatcagt accgccggaa atcgatcgtg accgatcagg
    13621 ccctcaccac gttcggcgtg cacaacgagc tcctgcgaat cctgtccgag acaggctggc
    13681 ccggactcgc cgcctgcctc tggttcgtgg ggtccgtggc cgtcgtcggg ttcaggacct
    13741 tcctcgaccg gccgggatcg gccgagggcc ggctcgcgct cggattgatc ctcggtctca
    13801 ccacgtacgc ggtccacagc ctcttcaact cctacctggg catcgacaag atcaccacgc
    13861 ccttctgggt cttcctcggg atgaccgcgg cgctcggaag cgcgaggacc tcgaacgcgg
    13921 ccccggacga gaaggacgcg gcgtgagggc gaggatgcgc cgctgagcgt catcctcatc
    13981 gtcgcgaacg cgctcgccct ggccgccgcg tgggccctcg cggcgaggct ggtccccgag
    14041 ggtcgtggca ccgcggtggt gcttggattt gcgctggtcc tcgcgtcgct cctcgtggtg
    14101 agcgtcgagg tcgtgggcgc cttggggctc ctcatcgcgc ccatcgcgct cccgggtgtc
    14161 gcatcggcgc tcgcgctggc cctactcatc tcgacgcgcg tcgttccgcg cgagccatcg
    14221 ccggccggcc agcccgccgc ctccgcgagc accggcacgg gaccgggtcc ggtgcacgtc
    14281 gtcgatcggg tcgcaagtcg cgaggcccgc gcgatcgggt tgtccgcggt ggtgctcctc
    14341 ctgacctggc tgccgctcgc ctggagccgg ctcgcgcttc cgcccgtatc gtgggacgcg
    14401 ctcacgtatc acctcgcgtt tcccgccgga tggctcgagc gcggcgatct cgcgacgacc
    14461 gtccccgcgt ccggcgatgc ggcaaacacc ttctatccgc tcgtgggaca gatgctcctc
    14521 gcctggaacc tcgtctcgac cggcaccgac cggtggacgg tcatcgctca acttccattc
    14581 ctcctcgcgg gagctctcgc cgtttccggc atcgcgcggc atctgggcgc gacgcgaggt
    14641 ccggctctcc tcgccggcct cctcttcgcc gccacgccgg tcgcgctgcg ccagagcgtc
    14701 gaggtcatgc tcgacgtgga gcaggcggcg ctcttcgcga tggccgccta cttcgcgctc
    14761 cgctcccgaa gcgggagccc gggtgacgcc atcctcggat ggctcgcggt cgcactcctg
    14821 gccgggctca agtattcggg agccgttctc gcggtcccgc tcgtcctcct cctcgttgtc
    14881 tcgggtcttc gttcgggacg gagcggtgtc gcgagtctcg gcgtgggtct gatcgcggcg
    14941 ctcgcggtcg gaggcttcgc gtacgtgcga aacgccgtca cgtgcgggaa cccgttcatg
    15001 ccgctcgaag tgaccgtggg cgggcacgtt ctctggcccg gatcgtccca tgcggccgcc
    15061 tacttcggcc cgaacgcgcc gcgtctggga tggcgggagc tcctcctctc cccgcgctgc
    15121 gttctcgaga tgggggtcgt cttcctcccc gcgctcgcca tccttgcgat cggcgcgctc
    15181 gccttacgcg gctcgaagga acgcatgtgg ctcgcgtgga tcggagtcgc ggcgttcgcg
    15241 ctgtccgccc tcgtcctccc gtttcgggag caccggtact tcctgccggt cgtcgcggcg
    15301 gcctgcgctc tcgcgcccgg actccttcga ggcccgaggt cgaggacctg gttcgcgcgc
    15361 gcggtcccgg tcgtcgttct tctgcaggcg cccgtctcgc tcgcctacgt ggcgaaggac
    15421 gtcatggtcc tcggtccggc cggagcggcg catgcgttcg gccatggtgc ttcgaccgga
    15481 tacgagagct cccggtacga gcgatggctc tcctactgga gcacgcgcca tgagtgggag
    15541 gaccgcgccc acgcgcgcgc cgatctcagg gacatggccg ccgcgtgggc ctggatcgcc
    15601 gggcacacgg acctcgctcc cgcgatcgtg gcctatgccg gagcgaacac tccctatcca
    15661 ttcaccggtg aaggctcgcg caatcgcgtt ctcttcgtgc cgcgctcggg cgccgcggac
    15721 gggactacgt atcggtgggg aaagccgccc gcttcggtcc aagaacccga ttcgaccgcc
    15781 tggcgctcga acgttcgagc cctcggcgcc gcctatctct gcgtcgtgcg actcgacgcg
    15841 cgctacggcg ggagcggagc gtttccgatc gaagacgaat gggcgcgcgc ggacacggac
    15901 gggttctcgc cgctctggac gtcggagaac gcccggatct acggggtccg ggtccgctga
    15961 gcgagggacg ccctgcggcg tccgcgaagg agagcggcga cgagcacgaa ggggaccgcc
    16021 ggcacgagcg ggatccgccg gggatcgaag cgccacttgg accagaccac gagcaggagc
    16081 gcgcccgcca gcgcggcgaa ccagaccgcg aggcctggct ctccctggag cgccaacgtg
    16141 agcgcgataa ccgcggcgat gatgaaaccc gtgcaggcac gcacgaacgg gcggcgcatg
    16201 cgaaacggtc ccaggcgcgc gatttcgatg cgctcccgaa acgcggccgg gtgccgcctg
    16261 gcgaggaggg gatccatctc gtagcgctgg gcccagcgga tgggatccag ccatccgggg
    16321 tgctccctgg ggtgctcgac ccgtatcgcc ggatcgaacg ccgcgcgcgc cccctgctcg
    16381 agcgccgtga atccgaagtc ggagtcctcg cgaaagtaga cgccccgctc cggatcgaaa
    16441 aactgctcgc agtagccgcc gatccgctcg aagaacgatc gcaggacgat gagattggtg
    16501 gggaggaagg tgagctctcc cggctcgcgg cgccgcgccg gactcccgtc ggggaggacc
    16561 gtcgcgcctt cgagcacgtc ggtcccgggg tccctctcga gacgctgcgc ggcgagtgcg
    16621 agccagtcgc tcgacgggag gcagtcgtcc tcggtgaacg ccaggtagtc gcccatcgcg
    16681 cgcgccgcgc ccgcgttgcg ggccgcgccc ggcccacgcc gctcgcgatt cgagaccgtc
    16741 gtggcgctca tcgcgtcgag gcggcgcgcg attccggtcg aaggctccgc cccgtcgaag
    16801 ctgacgacga gatcccagcg ggatcgatcg agagtctgcg acgccagggc gtcgaggagc
    16861 gtgttcaggc gatggtcctg accgagagtg ggaacgacga ccgtgaagcg gagggcggtg
    16921 ggatcggcgg gcacgcggcc ccgggttcag cgagccgcgg gctggacgcg ctctagggcg
    16981 cgcacgtgat cccggaagaa ggccacggtt cgcgcgagcc cctcgtcgaa gggcatggtc
    17041 gcgcgccagc cgaggagcgc cgccgccttc tccgtgtcga ggcatcgccg cggttgcccg
    17101 tcgggcttcg tcggatccca gagaagccgc cccttgaagc ccgcgtggcg cgcgatcgtc
    17161 tccgcgagat cgcggatcga gatctcgaat cccgcgccca gattgaccgg gtcgctcccc
    17221 tcgaggcgct cgagggcggc cacgaggccc cgcgccgcgt cctcgacgta gaggaactcg
    17281 cgggtggcct tgccggtgcc ccagacctcg attgtgtccg cgcccgtctc gaccgcctcg
    17341 acgcacttgc ggacgagggc ggggatcacg tgcgagctct ccgggtcgaa gttgtcgtag
    17401 ggcccgtaga gattcacgac gagcacgttc gacgcgtcga agccgaattc ctgccggtac
    17461 gcctgcgact gcacgaggag catcttcttc gcgaggccgt acggcgcgtt cgtctcctcc
    17521 gggtagccgt tccagagatt ctcctcgcgg aacgggacct cggtgaactt ggggtaggag
    17581 cagatggtgc ccacgctcag gaaccgcccc acgcccgcgt ttcgagcgtg ctccatgagg
    17641 agcgatccca tcatcagatt tcggtagtag taggtgcccg ggtagcggcg gttggcgccg
    17701 atgccgccga ccgacgcggc gaggtgcagg atcgcctgag gcttcaggtc ctcgagcatc
    17761 cgctcgacgg acgactcgtg cgtgaggtcg tagtccgcgg agcggaaggt cacggcgttc
    17821 ctgtagccct gcgcctcgag ctcggccatg acgtgccgtc cgaggaagcc ggctccgccg
    17881 gtcacgacga tgcgggtgtc cttgttcatg agtctccttg gtggcgattc ggacccggag
    17941 tgtcccagag cccgccgcgg caagtcaagg cgacgcggca ggctccttga cggctcgaag
    18001 ctcgaactcg agattctcgg cgcgcacgac gaacgcgaag agctgctccc atcgcgcggg
    18061 gaatcgattc gcgagcgcgg ccgcgcccgt catccgccac aggcgcggga acaggatgcg
    18121 gcggcgcacg cgctcgaagc gggcgctcgt gtagagaacg ttcggactcg cggccaggaa
    18181 tccatcgaac gcgaatgcgc cgaggacgtg gcggtgcgtg ggatcgttgt aggcgtacca
    18241 gctgctgaag tgaggggttc gaatgcggac gagcgctccg tggcgcgcca cgcggtgcac
    18301 ctccgccatg acggccggga tgtcctcgag atgctcgatc acgtcctgca tgacgatcag
    18361 atcgaacgag tcggacggaa gagggtaggg catccgatcg agatcgtgga tcacgtccgc
    18421 ctgggaggcg gggctatggt ccacaccgac gatctcggac gcctgggacg cttcgggaaa
    18481 gtcccgcttg gcatgaccgc agcccaagtt gagcacgcgc atggagccgc agtctatcca
    18541 aggcgagccc cgcccggcca gggccttgac cccgggacgg cccggcccca ggatcacggg
    18601 gtgtcgctcg ccctcgagcg cggggccgcg ctctcgttcc tccttctggc cgccgcgctt
    18661 ccctggtcca tcgcgcccat gtcgatcgcg gtcgcgctgt cgggcgcgct cacgctcgca
    18721 tgctggtggc gccccggagg cgcgcgctgg gtgcgcacgc cggtcgatcc ggccgccatc
    18781 gtgtggctga tcgcgctcgg gatcgcggcg ctcgccgggg aggatccggc ggggagcgtg
    18841 aatcgcgtca cgaagggatt ccttctcgcc atcgttcctc ttgcggccta tcacggacgc
    18901 gatcccaagc tcgcgcggcg cgcgatcgcg gtgctcctcg tttcggcggc cgtcgcgacg
    18961 gtgttcgcgc tcgtgaagtt cgccatgcag gggggcgcgt ttccggtgcg cgtgcggggc
    19021 gcggtggggc atccactcac ctacggcggt caggcgatgc tcctcgcggt gctcgcgggg
    19081 gcgatcctcc tccgctcgcg cgaccggcgc tggaagggcg cggccggcgc gctgctcctt
    19141 ctgctcgccc cggcgctcct cgggagctac acccggagcg cgtggatcgg caccctcgtc
    19201 ggattcgcga tcctgatcgg cttcacgcgc gcgcgatggc tccccgcgct cctgggagcg
    19261 cttctcgtcc tcgtgctggt cctgcctggc ggctaccggg agcgcgcgct gagcgtgttc
    19321 catccgcacg gcgtctggaa ccgcgagcgg atcattctct gggacgcggg gtggcggatg
    19381 tttcgcgacc atccggtgac cggagtcggg ctcgaggact tgaagccctg ggtggcgcga
    19441 taccgctcgc ccgaggcgca cgagccgccg cacggccaca tgcacaacgt gtatctgcaa
    19501 gtgctcgtca cgatgggcgt gatcggattc gcggcgttcg tctatctggt cacggcgctc
    19561 tggcgcaccg ccgcgcgccg tctccgcgtc gatgcgcgct ccctagacgc ggatcccttc
    19621 ggcatggccc ttcggatcgg gaccgtggcc gcgctcgcgg gattcctcgt cgcgggactc
    19681 ttcgagtgga atttcggcga cgaggagctt ctcgatttcc tgttcacgct gatcggcctc
    19741 gcctgggcgg cgagcgcctg gaggcacgta cgccccgtcg cgccgccctc gtcctgacgc
    19801 cgcggcttcc gtggccggag gatgacgggg gacggatcgc cacggcccag tcgatctggg
    19861 cggcggcgca ggagtacgac gtcacggtga tctcgttcgc gcaggaagcg cccggcccca
    19921 cgcctgagcc gtggcttggt ctcggcatcg acgtgaaaac ggtgccgcat cgcccgccgt
    19981 ccaagcccgt cgccctagcc caggggctct tggggaagct cccctacacg ctcgcgcgct
    20041 accggagcgc ggcgttcgcc cgtgtcgtgg acgagtgcgt ccgcaccctt cgccccgcgt
    20101 tcgcgctcgc gaacaacctc cacacggcct cgtatgtcga tcgcctcgac ggcgtcccgc
    20161 tcgttctgcg cgagcaaaac ctcgagcacc tctggatgag ccgctacgcc gaggagtcgc
    20221 tcggcggcct ggcacgcgtg tacgcgggca tccaggcccg tcggctcaaa gccgccgagc
    20281 gagcgttttg ccgcgccgcg gcgctgacgc tcgcgatcca ggatctggag cgggacgcgc
    20341 tgcgagcgct cgcccccggc gcgtggatcg agaccttgcc ggtcgggatc gatctcgacc
    20401 gattcggtga gccgcggacc gccgatccgc cgatcgtgct cctagcgggg gcgttcggat
    20461 ggccgccgac cgcgtcgggc gcgatccggt tcctgaagga cggttggccg cgagtgcgcg
    20521 cggccgttcc gggcgctcgc ctgcgcgtgg tggggaagtc gcagccggac gacctcgtgc
    20581 gcgccgcacg cgctctcggt gcggacgtgg tgaccgtcgt tccgtcgatg gccgaggaga
    20641 tggctcgggc ctcggtgatg gtggtcccgc tctggagcgg cgccggggcg cgcgtcaaga
    20701 tcatggaagc cctcgcggcg cgcctcccgg tcgtcgcgac gccgatcggg gccgaagggt
    20761 tagggctcga acaagggcgt catcacgtcg aaggggagag cgcgggggag ttgggggacc
    20821 ggacgggcgc gctccttcgc gacccggagc ggcgcgcgtc gctcgcgcgg gaaggccggg
    20881 cgtacgcgga ggcccactgg tcgcaggccg ccgtggcgcg gctccagaac gagctcgtgg
    20941 gacgaatcgc tcgatgaacg agtacgttct cttcttcctg tcggccgtcg tgtccgtggt
    21001 ggtgaacgcg ctcggccgcc tgcgtgggcg ctccgaaccg agccgcatcc tcgtcgtgaa
    21061 gctggaccat ctcggggacg tgatcctcgc gactccggcc attcgcgcgc tccacatgcg
    21121 ctaccccaag gccgtgatcg acgtgctcgt ccatcccggg ggtacggccg cggtcgcgca
    21181 tcatcccggc gtccacagcg tcctcacgta tcgctcgccg cgattctcgc gcgatggaac
    21241 ggccgacgat tcgtcgcgcc tccacgaagt cgcccggacg cggtacgaca ccgtcgtgga
    21301 gctgcgcggc gacgggcgga cgcttctcct gccgtttcgg accggcgccc gtcggcgcgt
    21361 ggatcgagga acggtgcgac tccgcaataa ggccctgcgc gtcctcggac gcgcgcctac
    21421 gctccacgag gtggagacca atctcgagat cgtgcggccg ctcgtcgggg cgccggcggc
    21481 tcgtccccgg gtcgaagtgg atcctgggcc cgaggcgcgt gcctcgctga cccggacgct
    21541 cgaggagcgg gccgttccgg ccggtgcgcg cctcgtcgtg ctccacccgg gcgccgcgtg
    21601 gcgtccgcgc gcgtggcgcc cggagcggtt cggggcggtt gccgactgga ttctcggcca
    21661 ctatgatgcc caggtcgtgc tcgtcggctc cgaggacgag cgggatgtcg aggacctggt
    21721 gcggcgctcg atgcgggagg atcgggctct ggcgatgacc ggctcgctga cgctcgagga
    21781 attgacggcg ctgctcggtc gatcggccct cttcatcggg aacgacagcg gccccgccca
    21841 cctggcggcc gcctgcggcg taccgtccat cgtgctcttc ggaccccagg acccgcgccg
    21901 cttcggtccg tggtcggagc gcacccaggt gcttcaccat ccggtcgcgt gctttccgtg
    21961 ccgccagacc gtgtgcgtga tggccgagga tccctgcgtg aaccgaaacg aagtcgccga
    22021 cgtgatcgcg agagcgcgcg acctcctggg gccgcccgag cgcgtccaag ccgcggcgcg
    22081 gggctgagcg tgttcgcccg gcaccgccag ctcctccaga tcggcgtctt cttcctcgac
    22141 gcgctcctga tcttcgcggc gtggtgcgtc gcgtactggc tccgcttcca cgcgctccac
    22201 ctggcggcgc cgcgcggagt gcctccgatc gagcgctacc tctggatcgg ggccgtcctc
    22261 acgccgttcg ggctcttgat cctgcgctcc ttccgcctct accgctcggc gcgcaccgcg
    22321 cggctctcgc aggagctctg gacgctcgta cagggcgtgg cgatcctcac ggcggtggcc
    22381 gcgctcgcgt cctattcgat tcgcggagag gtcgcgcgct cggtgctttt gatcttcgcg
    22441 gtcgtggcgg cgctcctcct gacggccgcg cacgcggcga tccggctcgc gctccgcgcc
    22501 gcgcgacgga gcggccacaa cctgcgccac gtgctcctcg tgggcacggg cgatctcgcc
    22561 gagcgcgtcg ggcggaagat gatcgcggag gcggactacg gccttcacgt gatcgggatg
    22621 gtctccgcgg acgccacgga agtggggaat cacgtcgggc cgttcctcgt ggtgggctgc
    22681 gtcgcggatc tgccggggct cgtgaccgag tccggggcgg agctcgtgta cgtggcgttt
    22741 ccgcgcgcgg agcacgaggc cgagcaggac gcgctcgagc ggctcggcga ctcgacggcc
    22801 gccgtccgcc tcgtgcccga tctcaaccgc aggcacaccc tgaacgcgag cgtcgaggat
    22861 ttcgacggca cgccggtcgt gctcgtgacc gagacgccgg agcagggctg gaactcggtt
    22921 ctcaagcgcg cgttcgatct cgccgtctcg gcagcgtccc tggtcttgct gtcgccgctt
    22981 ctcgtcgcga tcgcgctctg ggtgaagctc gactcgtccg gccccgcgct ctacgcgcag
    23041 gagcgcgtgg gctcgaacgg acgccggttc cgcatgctca agttccgcac catgcgcacg
    23101 gacgccgaag ccgcggggcc gcagtggagc caggccgagg atccgcgccg gacgcgggcg
    23161 ggcgagatcc tccggcgcct ctccctcgac gagctgccgc agctttggaa cgtcctcgtg
    23221 ggccacatga gcctcgtcgg cccgcgcccc gagcagccgg tcttcgtcga ccggttccgc
    23281 gcgtcgatcc cgcgttacat gctccggcac cacgtgaagt cgggcatcac cggctgggcg
    23341 caggtgaacg gcctccgcgg cgacacgccg ctcgagcggc ggatcgagta cgacctctac
    23401 tacatcgaga actggtcgat gagcttcgac atgaagatcc tcgccctcac cgtcgttcgc
    23461 atcttccgcg acccgagcgc gaactaggag gcgttggcat ggcgttacgg ctccacgtgg
    23521 gctgcggcac cgtgtacctg gacggatacg tgaacctgga cgtcgaggtg ccgggctact
    23581 ccttcctcgc gaccgagcgg cccgatctcg ccgatcgaaa ccgcacgacg gtcgaccgct
    23641 actacaagaa ggaggagtcg caggcgacgc tcgagcacgg accgggcgag atccagtact
    23701 gcgtcgtgga tcgcttcgcc gatcttcgcg cgcttccgtt cgcccccgat tcggccgacg
    23761 agattcgaac cgtgcaggtg ctcgagcacc tcgacatgcg ggaagcgccg cgcgtggtgc
    23821 gacactggta cgaggtcttg aagcctggcg gaatgctcca cgtggacgtc cccgatttcg
    23881 aggagaccgc gcgccagctc ctcgcgcagc cggacgagcc gtcgaaggac tggtactacc
    23941 ggctcgtcta cggcagccag aagaacgcgt acgcgttcca caagaacggc ttcagcccgg
    24001 cgcgactcga gcggctcctg cgcgaggccg gcttccgcga ggtgcgcccg gtcccgaacc
    24061 ggatccactt ctacccggtg gcgatcgccg aggcgatcaa gtagcgcggc cgcgagcgac
    24121 cccgaaccca gcgtgcgcat cttcctcgtc ctccaccgga gccccaaccc ggcgatcacg
    24181 tcggacctct ggaaggacaa cctccacgat ccgctcgtcg cgatggggca cgacgtcgtg
    24241 ctctggggag agggaatcgc tccgctcttc gatctcgacc ccgacgatcc gggaacggcg
    24301 gagccccggg cgcgcttcac cgagcgcttc ctcgaggcgg tcgaggaggc ccatcgggcc
    24361 tcgccgctcg acctcgtcat cacgtacgtc atggacagcc atctcgagcc cgccgcgatc
    24421 gatcgcgttc gcgacaagat cgcgccggtc ctcaatttct tctgcaacaa cgtgcaccag
    24481 ttccatctcg tgcagaggaa cgcgcgccac tactccttct gcctcgtacc ggaggcggaa
    24541 gcgatccttc gctacaagcg ggcgggggcg gagccccttt tctttcccat ggcggcgaac
    24601 ccggaccgct accgcccgat ggacgcctcc taccggtacg acgccacgtt ctccggacag
    24661 cgctacggcg atcggacctc gtcgatgctc gcgcttcgtg agggaggcgt ggacgcacac
    24721 gcgttcggcc agtactggga tccggcgccc gttcccgagg actcatccgc cggcttcggg
    24781 caacggggga ttctggcgcg cgctgtggag ctcgcgttcg gcggccggaa cgtgctccag
    24841 gcgatgcgcg accggatgga ctgggaccgg ctccggacgc gccatggagc ggcgctccac
    24901 ggtcccgtga gcgacgagga gtacgtggcg ctcttcagcg agagccggat cagtctcggc
    24961 ttcctcattc ttggagacac gcatcgaacg tcccggccgc tccggcagat ccgcctccgc
    25021 gagttcgagg cgccgatggc gggggcgttc tacgtcacag gatggctgga cgagatcgcg
    25081 cttcactacg agatcgggaa ggagatcgtc tgctaccggt cgagggacga gctcgtggac
    25141 ctgagccgct actacctcgc gaacgacacg gagcgcgaac ggatccgccg cgcggggcac
    25201 gagcgggcgc ggcgcgacca cacgtggcgc cgccggttcg aggacctgtt cgctgagctt
    25261 cggcgccgca aggtgatcga gagcgtctga gcgggctcag gcccgggtga tcagcgcggc
    25321 gagtcccgtg aggagcgcgg ccgcggcgcc ggccgccgca aggtcgccgg cgcgcggccc
    25381 tattcccgcg cgccgcatgc gccagatcgc gaccaggagc agcaccccgc cacctgccgc
    25441 gtaggcgaag gggagcgaca tcgcgcccgc tcccgcgcgg acgaacgcgg catacgcgac
    25501 ggcgagcgcg atcggccaga cggtgccgat tcccacccag agcctgcgtc ctccgacggg
    25561 cagggctccc accgaccacg tgagcgcggc gatgcgtccg aactctccga gaagggccca
    25621 ggccatgagc gggcgcgccg catcgaagcg cgtcgagtag aggagatgga ggagcggcga
    25681 ggccgccacc atcgtgaacg cgatcgccgc cgccgcgaga aggaggagcg gcttccattg
    25741 acgccttgta tacgtccgcg cggcgtccac gcctccggcc gcggacgccg cgctgatctt
    25801 cccgaacgcg taactggcca ggtaggcgta gaagacggcg ccgacgatct gcgacagagc
    25861 gagagccgcc tgcaacagtc cgttcgcgtc cacgccatga gcccgaacgt aatgcgcgcg
    25921 gagcgcgagc atcgtgccga gatcgaggag cgccaagaag agcgccgatg tgccgatgcc
    25981 gaggagccgc ttcgccacct ccccgctcac gatcgggcgc gtcggtggtc ccggcacgtc
    26041 gcggtacgcc cggcctcgca cgaggagcac acccagcgct cccgcgggcc agaacacggt
    26101 caccccgatc gtggccccca cgagtcccgc gcggggcacg aggaggaagg tggccgtgag
    26161 cgaggcggcg ccgccgaacg ccgcgtagat cacgggggcc gtcacgtccg agcgtcccgc
    26221 gaagagtccg agcaggagat tcgcgatcgc atagccgacc aggccgagca ccgaccagcg
    26281 aacgagatcc gcgtgcgagg gcgagtccag gagcgctcga gagagcgcgg gggcgaggag
    26341 gagggctgcc gtcgcgatca cgaggacggc ggcgaggacg aggccgaacg cggtccagaa
    26401 gccgcgccgc gcgccctcga cgtcgccgcg tcccaaggac tccgacaccg cctgcgcgac
    26461 gggcaggctc atgccgagcc cggcggcgat gccgagccac gtctgaccct ggacgatctg
    26521 cgccagcacg ccgatgccgg acacgtcgag gtgcgtcgcg acccacttgt tgcggcccac
    26581 gctcgccagc gcgaccacgg ccggataggc ggcggtcgcg gcgagcctcg cgccgcgctc
    26641 gatccagagc cgcgcgccgg gcggcatggc ggagagcggc gcctcgaacg gagacggcgc
    26701 gcggttgggc tccggcttga gcggcatgcg cggcagtgtc gaggaccctc cgcggagtgt
    26761 caatcgcgcc agcgggcgcg tgctacattc ggccgatgaa tgcggacccg gccgctcccc
    26821 tcgtgagcgt cctcctcgcg agccgaaacg gagcgcggtt cctacccgaa gcgctcgcga
    26881 gcatcgcctc ccagacctat cccgcgatcg aaccggtgct ggtcgacgac ggctcggccg
    26941 acgggacgcg cgcgatctta gacgagttcg ccgcatcgca cccgcgcgcg cgcgtgctcc
    27001 gcacggatgg agtcgggctc gcgtcggcga tgacgctcgc cgcccgcgag gcgagcgggg
    27061 actacctcgc gcggcacgac gacgacgacc gctcgcatcc cgagcgcatc gcccgccaga
    27121 tgaaatacat ggaagagcat cccgacgtgg gggtgctggg caccgcggcc caggtgatcg
    27181 acggtgaagg acggcgcatc ggaacgcatc cggtgcctct cacgcccatg gacatcggac
    27241 gaaccctcga gcgcgcgtcg ccgttcgtgc acggctcggt cgtgatgcgg cgccgcgcgt
    27301 acgaggctgc cggcgggtac cgcggggcat ttcgcgcctc ccaggactat gacctctggc
    27361 tccgcatggc gccgcacgcc gggctcgcca atctcgcgga gccgctctac gagtggcggc
    27421 tccacgacgc gggcgttttc tcgcgcgcgc ggcgcgatca gctcttctat gcggccgtcg
    27481 cgcgcgcgtt cgccgacgag cggcgcgaca cggggtccga ttccgtatcg ctcctctcga
    27541 acggcgcgga tccggacgga ctcttgagcc gctatccgcg cgcgggacgg ctggcgctct
    27601 atctcggcga agtccacgtg cgggaggggc tcggctcgga agggcgccgc tatctcggcc
    27661 gcgcgctgcg cgaatcggaa ttcggagcgc ttccctggtg gctcctcgcc tggatgattc
    27721 catggaccaa gcgcgggcgc cgggcctcgc gccgcgctcg cacgtgaaga ttctcctcgc
    27781 cgcggacgtc gatctctcga agccgggcgg gctcgagact cacgtgctcg aggtctcgga
    27841 gcggctccgc gcgcggggcc acgatatcga cctctgggcg cgaggcgtcc cggatgcggt
    27901 gcgcgcgctc gagccctcgc gctacgacat cgtccaccgt cacacgatgg cgtggccacg
    27961 gagcgtggag ccgggtccgc gcgtggtcac gaccctccac ttctgcgtgg cgggcaagat
    28021 ggacgcgtac gtgcgtctgg gccgctggaa gacgctcgcg cggccgggca actggaccgc
    28081 gcgggcggac gaagggcgcg cggcgcgagc gccaggcgcg ctgatcgcgg tctcgaagcg
    28141 ggtccgggac gagttcgcgc gcttccacgg catcgacccc gcgcgcgccg ccgtgatccc
    28201 gaatggggcc tccttcgcgc cgccgcgcga ggggcgggag gcgtggcgcc ggaagcacgc
    28261 catcgacccc ggcaccctcg ttcttctcac gatcgggaga cacgacttcg tgaaggggta
    28321 tgaccttctg gagcgcgcgt ggcgcgaggt tcgatcgcgc cactcaggcg ccctttgggt
    28381 cgtcgtgggg ggaagcggcc ccgagcgatc ggagggccgg ctcgtgacgg gatcggtgcc
    28441 gcgcgaccac gtgaccgagt ggatccacgc atccgacctg ggcgcgcttc cgtcgtatta
    28501 cgaaggctgc agcgtcgcgc tcctcgagat gctggccgga gggctcatga cgctggcgca
    28561 cgatgtcggg ggggcgctcg aggcgatccg cccgggcgag aacggcgagc tcgtgccgcg
    28621 cgacatgaat gcgtgggtcc acgcgatcga ccgtgcattt cgccaatcgc cgccgcgtcg
    28681 ctccgggggt ctagacccgg cgtatcgatg ggacgcgatc gtggattcga tcgaagaggt
    28741 ctatcggagg gtgcgtgccc gatatccgtg atacactgcc gcccatgcga gtcgtctttc
    28801 tcacctcgcg cctgcccttc ccccccgaag ggggcgatcg cttccgcgtc taccaccaga
    28861 tccgaacggc atccgaagcc ggtcacgagg tccacctcgt gacgttcgcg cgcgagcatc
    28921 ccgatccctt gtcgatcgcg ccgctcgagc gcctcctcgc gtcgatcacc gtggtgccgc
    28981 tgcctcccct gctctcggcg ttccgcgccg ccaaggcgat cgcggggcac gcgccgttcc
    29041 aggcagccta ttaccgctcg cgccgcatgg aggatgaggt tcgccgcacg ctccgccgtg
    29101 tgaagccgga cgtggtcgtg acgcatctct tccggatggc tccctacgcg ctcgcgaatc
    29161 agggcacgtg ccaggcgcgc tggatcctcg atctcacgga cgtgatctcg gtcggcatcg
    29221 cccggtcgct tccgttccgg aaggggcccg atcgctggct ctaccgcgaa gagcgccgcc
    29281 gtatccggcg gtacgaatcg gagatctcgc cccaattcga cgagtgctgg gtgatcagcg
    29341 acgccgaacg gaacgagctc acgcggatcg ctcccggcgc cgacgttcgc gtcgtgccga
    29401 acggactcgt gcccggctcc cgcctcgacg gaaacgcgcc cgacaaggcg cggctcctct
    29461 tcctcggctt ccaccaggta ttccacaacc gcgacgcctt gcgcttcctc gtgcaggagg
    29521 tcctgccgcg cgtccgcgcg agcgtgccgg acgcgacgct cgacgtgatc ggagcgggcg
    29581 cggaggcgct cggaccatgg gcgcgagggc ccggggtgca cgtgctgggg cacgttccgc
    29641 ggctcgagcc ctacttcgtg cgggccaccg tcttcgtcgc gcctcaccgc ttcgcggcgg
    29701 gcgtgcagaa caaggtggtg cacgcgctcg cgaacggcgt tccggtcgtt tcgacctcgg
    29761 tcgtccggag gggcctcgag cccattccaa gaggcttgat cctgaccggg gagaccgccg
    29821 acgagatcgc ggcgcgcgtc gtcgatgtca tccaccaccc ggcccgcgcc gcgtggatgg
    29881 ccgagcaggg gcgcgagtgg gcgctcgcca cgttcagctg gcacgccacg ctcgaagcgc
    29941 tcgaggccca cgacaccgat tccgcggctg acgcggaacc gctcgtctcc tcggccgtgt
    30001 gatcggccct ccaccgaacc tcgtcttcgc cccgctcgcg ttcggctgcg ccctggcgct
    30061 gacgccgctc ctcggccgca tcgccgaggg gaccggctac ctcgatcatc ccgatctcag
    30121 aaaacaccac gcacagccga cgccgctttt gggcggcgtc gcgatcggcc tctcgatcgt
    30181 ctccgcgctc cttctcgcga actacgtgtt cgagaggacc gtgaacgtgc cggcgccggg
    30241 gatcatgctc ggcgcggcga tctcgctcgt tctcggattg atcgacgact ggcgtccgat
    30301 ggggccgctg ggaaagctcg tgggccaggt ggccgcggcg gcgagcctcg tcctctgggg
    30361 acctcgcatg gagtggctgc acggcaatcc gatcgtggtc gcgaccgcga tcgtcgcggt
    30421 ggtcgcgctc ttgaacgcgg tcaacttcct cgatgcgatg gatggaatcg tcggcaccgt
    30481 gctccccatc atcggagccg ggttcgtcgc gctggcggtg ctctatcaag cgcccctcgc
    30541 gaccgcgctc ggatggtcga tcgtcggcgc gtgcgcggga ttcctcgtct acaacgcgcc
    30601 cccggcgcgc atcttcctgg gcgacgccgg aagccatctc ctcggcttcg gtctcgcgac
    30661 gctcgcgctc caggcgctcc gggacgagcc cacgctgccg cacgtcgcgt ccgtcaccat
    30721 cctcgttgcg tatccacttt tcgacgtcat gttcgtcatg tgggacagaa gtgcccgtcg
    30781 gaagccgatc tatgtcggag gtgtggacca cacgacacac cgcttgagcc gacggtgggg
    30841 cgcttggccg acactcggcc tcctcgcgct gctcaacgta atccatgtag cactcgcgct
    30901 atgggtctgg ggcctctccg gaccgcaagc cgtgattccg cttgtcctca tttttgggct
    30961 tggatacgcc attttcgggg cctacctgag acgtttgagt cccaccccct agttcgacat
    31021 ttgacaaacg gcttttacag ttgtatagtc cgtcaagcaa atccacaggg gagccggtgc
    31081 catcccaccg gtgtggtttt cgcgagggcc aaacgacatg tcacagcgcc ctgatccaat
    31141 gcacgagaac gatccaacgc ctgaattgcc ggaaatggac ccgctcgaac gtctgagccg
    31201 ggggctcaca caatgggaac gtgagcttct ggcgcagccg cgttcgccgg cgcgtccgcg
    31261 cgcgtccacc gatctcgagc gcgtcaacaa cgtgctccag gcgagccgcc aggttaccgg
    31321 cacgctcgac tggaacgagc tcctgatacg cgtcgtcgac gccgtcgttc agatcgccgg
    31381 cgccgatcgc ggcttcctca tgcgcatgcg cgaggatgga aagctccact tcgagatcgc
    31441 gcggagccgc gacgaggcca cgctgccgcc ggacgagttc cagatctcct ggggcatcgc
    31501 cgaggaagcg gcgcaccgcc gcgagaccgt ctgggttccc gacgccgtcg gctcctccct
    31561 cttccaggat cgaaagagcg tccgcgagct ctcgcttcgc accgtcgtcg ctcttcccat
    31621 cctgaccgcg gggcgcgtgc tcggcgtgct ctacgtcgac tcgcattcga tcgcgcacga
    31681 gttcactccc gaagacatcg cgattctcga aggcttcgcg gcgcaggtcg cggtggcgct
    31741 cgagaacgcg cggctgcacg aagagctcaa ggattcgaag aaccgtctcg agatggagaa
    31801 cctgaatctc cgccgcgcgc tcaaggagga gtcgcggtac ggcctcctcg tgggccggag
    31861 cccgaagatg ctccgcgtca tcgagctcat ggagaaggtc atcccgacgc aggtgtcgat
    31921 cctgatccag ggcgagacgg gaaccgggaa ggagctgatc gcccgcgcga tccatctgaa
    31981 cggcccgcgc cgcgagaaga acttcatggc ggtgaactgc ggcgcgctgc cggaatcgct
    32041 cctcgagagc gagctcttcg gctaccggaa gggtgcattc accggcgcgt cggaagaccg
    32101 catcggcctc ttcgaagcgg cggacggcgg gacgttgttc ctggacgagg tgggggagat
    32161 gcccgccacg ctccaggtga agctcctccg cgtgcttcag gactcgcaaa tccgtcgcgt
    32221 cggggacacc gtgtcccgcc gcgtggattt ccgtctgatc gccgcgacca accgcgacct
    32281 caagaccgag gtcgaggctg ggcgattccg ccaggacctc ttctaccggc tcaacgtggt
    32341 gccgatctcg ctgccgccgc ttcgcgagcg gggcgaggac gtgctccttc tctcgcagca
    32401 cttcctcgag ctcttctcga agcagcagga gaaaccggtg cgcggcctga gcgcggaatc
    32461 gcgggagctc ctgctccgtc atccgtggcc ggggaacgtc cgcgagttgg agaacgccat
    32521 ggctcgcgcg gtcgcgctgg ccgacgaggg aggcgccatc gagccgctcc tcttcgggct
    32581 gggcgcgccg gtcacgcggc gctgggacgg ccagcacacg ctgcgggaga cgctcgacgc
    32641 cgtcgaggcg gagacgatcc gcgaagcgct gcgccagtgc gagtcgaacg tctcgcgcgc
    32701 ggcgcgtgcc ctcggcgtga gccgccagca tctccacaac cggatgaacg cgcacggcat
    32761 ccgccgctcc cggaccctga gcgagtccgc cggataggat cggtgtcgtc cacaatcgct
    32821 ccccgcgcga cgtccgggaa ggacgcaatc gcctcccgga ggcttagctc gctccgcgcc
    32881 atcctcatgc agagcgatgc cgagatccgc gcgcagttcc ccgagtactt cctcacgtat
    32941 tacacgtacc tccgcatcat tcggaacccc gcgcgcatcg agcacctctt ccagaagggg
    33001 tacgacatgt accgctacct cgaagccgag gggaaggacg tgctcgatct gggggcggga
    33061 ttcgggatcg aggcgctcct cgtcgcgatc tacggcgctc ggcgcgtggt ggcgaccgag
    33121 atcgaccacg acatggtcgc ggtcgggacc tatctcgcgg agaagatcga tccgccggcc
    33181 acccacttcg aatccaagta cggcgacggg atcgggatgg agttcccgtc cgcctccttc
    33241 gacggggtca tggccaactg cgtcatctcg cacgtgcggg atctcgaggg ctttctcgcg
    33301 gaggcgaatc gcctgctccg gccgggcggg atcttcttcc tgagcgacga gaacaactcg
    33361 ctctaccttc ccgcgcgcgg ccggcgccgg cgcggctggc acagcatgga gcacgagccg
    33421 gacggtcctt acttcgccgc ccggcgagcg atgatcgggg agcgctttcc cgggctcagc
    33481 tcgaaccagc ttctcgaggc ggtgcggcag acgcgcggcc tctggggaga gggcgtcgaa
    33541 gcgggggttc gggagctcgt ggagacgggc cgggttgagc ggaagcccga gttcctgtac
    33601 cgggatcctc gcgacgggca gtaccccgag cgggagctca atccgttctg gatgatgcga
    33661 aagttcaagg acgccggact cgagccggag ctcttgccgg cgttcttctc gcgcgccatc
    33721 gaggtgcatc cacggcagtg ggtgaaggat ttcctccggt tcttctgcgt gcggtggccg
    33781 gccttgtcgg tctacgtctg gcccatcatg cggctccgcg ggcgaaaact gcgttgacac
    33841 tgccttgtaa cgtcgcctag gctgcggcct agtgaagtcc gtgagcgtca ccttgatcgg
    33901 gcttttcctc gcggcgaatg ccttcgccgc ccctatcgac gcgcccgcta ccccgcctcc
    33961 cgaccttccc atcccgcaga acctggtcgc caaggacaag ccaggagact ccggtgggtc
    34021 cgtcgtcctg acgtggacgg acccggtttc gcttcccgcc ggggcggaca tcgaggtgct
    34081 ccgggcggag ccgccggcct acgacctgca gccgatcgat cacgtggcgg ccggcaaggg
    34141 gacgtatacc gacacttcgg ccaaggacgg cgtggcgtac cgctacggcc tccggaccag
    34201 ccttgcggcg accgactcca cgggggcggt cagcgtggcg gccgcgaccg gcgctccggt
    34261 cgtgagcgag cccgtctcct cgcacgacaa ctggttcaag gtcgagcgca ccaactcctt
    34321 catcgcctcg atcctgttcg tgatcatcat cctcggctcg atccaggtgg cgcgctccgg
    34381 caaggagatc ttcatccgcc ggatcgcagg cttgaacgcc atcgacgagg ccatcggccg
    34441 cgcgacggag atcggcaaga aggtgatgta catccccggc atccagtcga tggatgacat
    34501 ccagaccgtc gcctcggtgg cgatcctggg ccatgtggcg cgctcgaccg cgcgctacgg
    34561 caccgacctc gaggtgccga acaaggaccc gctcacgttc gcctccgcgc gcgaggtcgt
    34621 ccgcgcggcc tacctcgagg aagggaggcc ggatctcttc cgcgaggaga tggtgaacta
    34681 cgtcacgtac gaccagttcg ccttcaccgc ggcggtctcc gcgaagatga cgcgcgagaa
    34741 gccggccgcg atcttcctgg tcggctactt cttcgcggag tcgctcatcc tggccgagac
    34801 ggggcagtcg accggagcga tccagatcgc cgggcaggcc gatcccacgc agctcccgtt
    34861 cttcgtggcg acctgcgact acacgctgat cggcgaggag ctctacgcgg cgagcgcgta
    34921 cctctcgcgc gagccggtcc tgctcggctc catccgtggg caggacatcg ccaagggcat
    34981 cctcatcctc atcgcgatcg ctggcatcgt gctcgcgagc gtccacctgg tcgacatctc
    35041 cccctggatc cgaaccaaat gagcttcgaa ttcggacagg tcgctctcgt cctctgcttc
    35101 gcgatcgttc cgatcatcgg gatcgtgctg gggctgaacg cgcccggtct tcggagccgc
    35161 ggcccgatcc tgattggcgt cgtcgccggc ctcgtcccca tcttggcgat gtacatcccg
    35221 aagggcttcg tgtcgggacc cgagggaatg gggccccggc tcgacaactg gctcatcatc
    35281 ctggcggggt tcgccctgct cctcgggatc gtgaacgtgc tgcagaacaa ttttaagaaa
    35341 atcgcgcgcc gggagtccgg ctggttcttc tcggtcgttc tcctgatcgg gctcttcgtc
    35401 acgggagggt tcggagtcta cggggcgctc tcgagggtgg gcatcggctt caatccggat
    35461 ggctcgccga cgccgttcaa gtacatgacc gactggttct tccagccgct ccagtccacg
    35521 atcttcgcgc tcctcgcgtt cttcatggcg agcgcggcgt tccgcgcgtt ccgcgccagg
    35581 aacgtggagg cgacgatcct cctcatcgcc gccggcctcg tgatgctcgg ccgcgttccg
    35641 atcctcgagt tcctggcggt tccgttcccg ttcctccatg cggccgccgc gtccggcagc
    35701 caggggttgg ggaagctcac cgagtggatc atggacgtgc cgaacggagc ggcgcagtcg
    35761 ggcatcatca tcggcgcggc gctcggcgcc gcgtccatgg cgatccgcgt gattctcggc
    35821 atcgagcgcg gctacctcgg cctggggaag ggggagtagg gtgcgcgcgt tcttggaacg
    35881 gctcctccgc ctggatcgcc gctggatctt tctcttcgtc ctcctgagcg tcgcgattcc
    35941 gatcgtgatt ccccttgggc ttcccgtgac cacgaccgaa tccacgcgca aggccttcga
    36001 gttcatcgaa gccatgaagc ccggcgacac ggtgctgatc tcgttcgact acgggccgtc
    36061 ctcggccccg gagaacgatc cgatggccga agccgtcatg cggcagtgcg cgatcaagaa
    36121 gctccgcttc atcgtgatcg cgctctaccc gctcggaggg ctgaacctcg ccaacaacgt
    36181 cgtcgccaag gtcgcggccg agtaccccga tctcaagtac ggcgtcgatt acgtcaatct
    36241 cggctacaag gacggcgcgt cggcggtcat gcgccggatg ggggacgaca tcgcgggcgc
    36301 gttcccgacg gacgaggcgg gcactcccat cgcgcagatc ccgatgatga aggggatcgg
    36361 aagccttcgt caggtgcagc tcgtcttcac ggtggcgacc gggatcatcg gggagtggtg
    36421 gatcacgcag gtgcacccgc aggtgggcac accggtcatc atcggtccga ccgcggtgag
    36481 cgctcccaag tactatgcct tcctgaactc gggccagctc gtcggaatga tcggaggcat
    36541 gaagggcgct gccgagtacg agaagctcat gcgcgcggcc catcccgaca tggaacgctt
    36601 ctacaagtcg acccgcgact tcacggccac caaggggatg gatgggcaga ctgtgatgca
    36661 cacggtcgta ctcctcttta tcctgctcgg caatctggcg ttcttcctca ctcagaagag
    36721 ggcggtccgg tgagccatct gcccccgatc ggcgtctgga tcgcggcgtt cctgaccctc
    36781 tccgcgctct cgttcctgta caaggacaac ccgttcttcc gcttcgcgga gagcctcttc
    36841 gccggcgtgt cgctcggcta ctacgtgggg atcaccctcg accagaccct cgtcccgaac
    36901 ctgatcgagc cgatgcagca ggacttcggc aagcactggg atctgctcgt gccggggctt
    36961 ctcggggtgt tgctctacgc gcgctatctg ccgcggatcg gatgggtctc gcgctacgcg
    37021 ctggcgatct acgtgaccta ctacatcggg ctcgatttca cgcggcgtct gcatggcgaa
    37081 gccatgccgc agatcgcccg cgtcatcgtg gccctccccg ggccgaatcc gctcgcgttc
    37141 atcttcgtgg tgggcgtgct gagcgtgctg gtctacttct tcttctcgaa ggagcagggg
    37201 ccggtgacgc ggaggatttc gaggctgggg atctggttcc tgatgatctc gttcggcgcc
    37261 gccttcggct tcacggtcat gggccgcgtc gcccttctga tcggccgcct caacttcctg
    37321 attctggact ggatctaccc gattctcggg atttgatctc cgggtccgtc gcgggcttca
    37381 ccgccggccc ggggtcgggc gcgtccgacg acgccttgaa gttcaccatc ggcaccgtcg
    37441 tccggttccc cgccggatag tccaccgtca ccacgtcccg aagcgccgcg atccgctgcg
    37501 tgagacccgc gatcgtctcc gtcgccgccc ggatgcgagc caccttctcc cgggcctccg
    37561 gcgtcagcgt gggctgcacg tagaggagct gcgcgttccc ctggatcact tgcaggggat
    37621 tgttgatctc gtggttcagc gccaccgcca tggccgagac cgccttcatg cgctcggcgc
    37681 ggagccggtc ctcggtcgcg cgatgcgccg cgagcgcgtt ccgcaccttg atcgggagct
    37741 ggaaccgcac gtcccactcg gtgagcggct tcatgaagta gtccgacgcg ccgagcgtga
    37801 ggctccgctc cacgtcctcg ggggatgtcg acgcgctcgc cacgatcacc gggatctcct
    37861 tccagcggtc gtccttcttc atcgcgagga gcacgtcgaa gccgttcggc gacggcatca
    37921 tgaggtcgag cagcacgagc ccgatcgtct cctggcccag gatctcgagc gcctggttcc
    37981 cgttgtccgc ctcgaagaag acgtagtccc cctggcgcaa gaccatgcgc aggagcttcc
    38041 ggttgtgcgc ctcgtcgtca acgagaagca ggtgctgggc catgagcccc tttctccgcc
    38101 agagtgcgaa tgcgcgcgat cagctcgtcc gcgctcacga tcggtttcgc gatgtaatcg
    38161 tcggctcccg actcggcgag gaagcgctcg cgagagccct tcatcgcgtg cgccgtcgcc
    38221 agaaggatgg gagtgcgccg ggtctctgga tcccgcttca gccggcggca gatctcgaga
    38281 ccgtcgaccg gaacgccatc ccacgcgctg ttcgagagcg acacgtcgag gatcaccacg
    38341 tcgacgaggc cctgccgcac gcggtcggcc acgtacgccg gatcctccgt cacctcgacc
    38401 tcgtaccgcg ccttccgggt caggatgagg ccgaacagct tggcgttcat cgggtcgtcc
    38461 tcgatgatca ggatccgcac gactccgcct ccgttggagc cgcctgcgcc acgggcagcg
    38521 tgatccggac cacggtgccg cggttcacgc cttcgctcgt gagctgaatg gtgcccccca
    38581 tgagatcgac gagcgcgcgg ctgatcgcga gaccgagccc cgttccgcgc tggcgccggt
    38641 gataggacgg gtccgcctgc gcgtacttgc tgaagagcgc ggcccggcgc tccggcgcga
    38701 tgccgacgcc cgtgtcctcg accgacacga cgatcgagcc cgcgtcgatt ccggcgcgga
    38761 cccgcacctc tcctctatcg gtgaatttca ccgcgttccc gagcagattc aggagcacct
    38821 gccggaaccg ggcgagatcg accgccgcgg agagcggctc cttgggaacg tcgacccgga
    38881 ccgccaatcc cttggccgag gccatcggct ggaccgcgtc gaccgcgccc cggatcgtcg
    38941 gcgcgatgtc ggccacgagg agccggagct cgaaccgccc cgcctcagcc ttcgcgagat
    39001 cgagaaggtc gttgatgacg gagaggaggt gctccgcggc ctggcgaatg gaccgtgcga
    39061 agtcgaggcg ctcgccttcg tccgcgtcga tccgctgctc gagcaatgtg gcgaacccga
    39121 tcacggcgtt gagcggcgtg cggagctcgt gcgaggtgtt cgcgaggaag tcgttctgca
    39181 gccggcgcgc cagctcgagc tcgtggttcg cgtcgaggag ccgctcgttc atgcttccga
    39241 gctccgctcc gcgcgcgcgg tcctcctgct cgccttctcg gcgctcggtg atgtcgcgcg
    39301 ccacgaggac gatcgcggtc agcgctcccg cgcggtcgtg tgccggcgag agccgcacct
    39361 cggccgggaa cgtgctcccg tcgccgcggc gaaagccgag ctcgcccgag aactctcccg
    39421 atgcgcgcgc cacgtcgagc gcggccgcgg gacctgaagc gggaacgagc gcttcgccat
    39481 cggctccggt cgcgccgggg gcgaaggcca ggtcgtgaag ccggcgtccg aggagctcgc
    39541 cgggttgagc ggagagcgca agctcggccg cgcggttggc gctcgacacg actccgtcga
    39601 ggtcgagctc gagcacgatc tcgctcgtcc cgacgaggta ggaggcgagg atctgatcgc
    39661 gccgctcgag tcgaaggcga cggagcccgg atgcgagctg cgcggccatc cagcggagga
    39721 gcgcgcgctc ctcgttcgcg agcgaggcgc cggccgggag gaagcactgg agcgtggccg
    39781 ggagcccttc gtgctcgagg aagggaaccg agacgaccgc ggtatcggcg ccgagctccg
    39841 gcagcgtggc ggcaaacgca tcgcgctccg agcccgcgag gtcgcccacc acgatctcac
    39901 cgagccaggc cgcgcggctc gcgtgcgccc gcgcctccgg tcccggcgcg tccggctcga
    39961 gggcgacgtt cgcctcgggc gtgaggccgc gcgtcgccac gagcgtgaac tcgccgcggc
    40021 cgccccggtg cgcgtagagc gcccagtgtc gaagaccggt gccttcggcc accgcgtcga
    40081 gcgcgtgatg gaccaggtcg tcctcgcggt cgaacctcgc gagggtatcg ccgacccggt
    40141 agaggctgag ctgcgcttcc cgcgcctggg cgcgatccag gtcctggacg cgcgtccgat
    40201 ggagccaccc ggccgcgagg atcaaggcca gaccggcagg ccaagcagcc cagggcacac
    40261 tcgaggacga cgcgatccac ccgaccgtga ggcacgcgag aagggcgccc aagatggcga
    40321 agacgaggga gcggcgcttg gcgcgggcga tcccggcggg gctggatacc cctggcgctg
    40381 cgagcgatgg gttgtaggtg ctcggcacgg aggcctcggg taggagggac ggcgccaggg
    40441 tccgttcggg actatcggca cccgagacct ggaactggag ccgccgcgcc gggtttctgc
    40501 gggtggtgct tcctgctagc gtccaatgcc gcaaggggat atcatccgtc ctcgtgcgct
    40561 cccgcccaat cctcgtgtcc ctccttctcg ccatctggtc cctggccctg tccggttgtt
    40621 cgaacaacag cgaccgggtc acggggaacg agcgcctgat tcgtggcgcg ggggggctcg
    40681 ggtcgaccct ggtcgagacc accatcccgg accgggacac gtacgtgaca cccgggaccg
    40741 ccaactacgg ctccgtcctc ctcgtggggc gtgacgcgac gttcgaagcc cgcgcctttt
    40801 tcaagatcct gcgcatcaac ctgccgagca ccgatttcgt gagcgtcgta ccgaccggga
    40861 acgtgctgct cgacttccct cacgtcccgc tccgcaatga gcccctttcc attgatctcg
    40921 agctgcgtga gacggccacg gctctggccg atagcggaac gatcgcgtgg ccaggccccg
    40981 cgcctggggt tcttctcgcg tcgagcacct acgacttctc cggggatctg gtcatggatc
    41041 tcgggccggg gagctatagc cgcgtggtgc agtggacgac ccccgatccc g
//
LOCUS       DASTKC010000199         9644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_397657, whole genome shotgun sequence.
ACCESSION   DASTKC010000199 DASTKC010000000
VERSION     DASTKC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397657"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFR25_08345"
     CDS             <1..217
                     /locus_tag="VFR25_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941100.1"
                     /translation="SRAYEAYGRAYPDRDGAALALLKSAEIEQHALNNPGRARFLYDE
                     LLRRPLGPELEQMVRERCELADTALAG"
     gene            complement(227..763)
                     /locus_tag="VFR25_08350"
     CDS             complement(227..763)
                     /locus_tag="VFR25_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9941101.1"
                     /translation="MMRPFLRFLGSVSLFLLATGPVQGAPPSASPPGSSNESPIAGTK
                     RIEEYQSLGLDRRLTPFTGTVLDLNDHPLSGVQVKVFNEGQELVSGITDGNGVYDLRA
                     PYDPASDATVLLWFAAPDHSLMTKEIVIHESRRSVENGIISRCIPRAPLTPGRQFRVY
                     LFDPASRTKELSELDCLP"
     gene            983..2020
                     /locus_tag="VFR25_08355"
     CDS             983..2020
                     /locus_tag="VFR25_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9941102.1"
                     /translation="MFLDQVMGVFSNDVAIDLGTANTLVYLRGKGIVLNEPSVVAVDR
                     TTNKVIAVGKEAKSMLGRTPDEIHAVRPLKDGVIADFEKTEDLLREFIQKALRRRTWV
                     RPRIIICVPSGITEVEKRAVQDSAQHAGAREVLLVPEPIAAAIGVGLPVGKPSGNMII
                     DIGGGTTEIAVMALNSIVNQQSIRVGGDEMDEAIVQYVKKAYNLLIGEQTAEQIKIRI
                     GSAFRLEQEEEMEIKGRDLVAGIPKTMKISSVEVREALSEPLQQIVDALMQSLEKTPP
                     ELASDIVDRGIVMTGGGSLLRGIDMLLREATNLPITVAEDPLSCVVLGTGKILDDPTQ
                     YEKVLMSVVRD"
     gene            2041..2997
                     /gene="mreC"
                     /locus_tag="VFR25_08360"
     CDS             2041..2997
                     /gene="mreC"
                     /locus_tag="VFR25_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET9941103.1"
                     /translation="MLRYLFVRRTDWTVLVIACTLSLSMMLLEARMQARVAWFLQHTL
                     LAPFEYSIGWVDQSVGVFWENHQLRSRLTQLQIESDALQAERIENVRLRRLLHLEERH
                     PYNLVAASVVARSLDRLGGSLTIDKGTADGVEPGRAVLTPDGLVGRAERSTPHETRVL
                     TLLNRDCAVAARIERTRVDGVLEWEFGSQPVLNLRYVSSQEDVKVGDRVVTSGLGGIF
                     PVGIRIGTVARVALDPNGLMKEILVDPAVDFRSVEEILVYTPSDLRGTAPADLFLEEP
                     KPAAPGDSTAADSAATRPATPSSKAIATTQPSESAAPEKVVR"
     gene            2994..3488
                     /gene="mreD"
                     /locus_tag="VFR25_08365"
     CDS             2994..3488
                     /gene="mreD"
                     /locus_tag="VFR25_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET9941104.1"
                     /translation="MKWLRDGFVLLVALVLQSTVLHRIEIAGVRPDLLVAYVVYIAWM
                     RGPVVGTIVGFGVGLIQDLDASVPLGLNALAKSLVGFLVAKAGFRVHRANAGVRFAFF
                     FIAMLVHDVIYYAVATDGDLAMFAKQMVLSAVPSALYTGVVVVILLGIVEAFSRKLLL
                     ADEV"
     gene            3490..5355
                     /gene="mrdA"
                     /locus_tag="VFR25_08370"
     CDS             3490..5355
                     /gene="mrdA"
                     /locus_tag="VFR25_08370"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9941105.1"
                     /translation="MAYEIEPRHQARRREQIFTIGVASIFGLLILQLFSLQVLQGDKY
                     GELSEENRIRVEVLTAPRGEIRDRKGRLLADNVPSFTVTIDPFDKVYVHDPALLDSTI
                     ARVSRIVGLDPEVAFDKVKRERTQSFLSIRLKRNLDLASVAYIAEHRAELPGVEVEAE
                     PLRRYPLGPFASHLLGYVGEVSDKELEDPKFASTYMRGDLIGRMGIERQYEKILRGVD
                     GKRFVEVNALGRKADLLGDRQPILPKRGEDITLSIDLDLQRAAEEAFQPGARGAVVAM
                     DPRTGEVLALVSKPNYDPNEFSTGITQARWEELSQGGNYPLFNRAIQAAYPPGSTLKP
                     FVALAGLTLRAITPVTTFADPCFGYYQFGRRRFGCWDHDGHGWTSLRSAVAKSCDVYF
                     YQLGLRIGLDRLSTFMKQFGVADRTGIDLPQERRGLLPSAAWYDKHYGAGRWSKGLIL
                     NLAIGQGEWSLTPVKLAQMTSVLASGGTLWKPHILRSVSRDGGEDRSAIPRADTLSTH
                     LDYPARDLSSVRDAMVAVVADGTGYQAKMDSITVAGKTGTAQNPHGQDHALFICYAPA
                     SRPEIVVAVLVENAGHGSTAAAPLAQKVLQAYFHPAPPDTTTAPKAQTVALRSGT"
     gene            5352..6581
                     /gene="rodA"
                     /locus_tag="VFR25_08375"
     CDS             5352..6581
                     /gene="rodA"
                     /locus_tag="VFR25_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET9941106.1"
                     /translation="MIASRLTADIDRPLFLTTILLALIGIAFVFSATAMQTAQVEHGL
                     YLKQFLWLGLALAGAAATAAVPYRIYEGKTAWLLYAITLVLLALTLVIGHVGLGAQRW
                     LGWGPLKFQPSEVAKIAVVLVLASMLAAKRIDLTHLPTLFKTLLAAGVPFLLVLKQPD
                     LGTSLCFIMVVLTMLYWAGLPMLYLFLLLSPILNGVLSFYLPAWILFALVLAFVLYRS
                     RLRLWTVVLVVVVNLAVGIATPHVWNHLEPYQKQRISTFLDPSADPYGAGYQIIQSKI
                     AIGSGGVTGKGFMHGTQKGLEFLPEQHTDFIFSVVGEETGFLGAAFVTVLYMVFILRG
                     VRIARRARNRFGSLLAIGLTSVFLYHVLVNVCMTVGLAPVTGLPLPLLSYGGTSLLTS
                     FLQIGLIQNVAMRWREY"
     gene            6596..7447
                     /gene="lgt"
                     /locus_tag="VFR25_08380"
     CDS             6596..7447
                     /gene="lgt"
                     /locus_tag="VFR25_08380"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9941107.1"
                     /translation="MHPTLFHIGSFQIHTYGLLLAIGFLIAIQIFVARGVRRGVAEDQ
                     LHTISLVILILAIVGGRGLFVLTHWSDYAKDWLGVFRLWEGGLMLYGGYILAIAGGIW
                     YVRRAKLPVWRIGDAAAPAMALGIGIGRLGCYFNGCCFGLPTHLPWAVTFPPGSYASY
                     AFPGEAIHPSQIYLALAGVFLFGLLLAMDRKAHFDGWLFWTAVAIDAVFRFGIDFTRY
                     YDSTSYLGRLGPLSFNINQILSAILFLTALVMLRVLSRRPAPSAAATGTEPGDETAAS
                     ALGTQAG"
     gene            7467..8333
                     /locus_tag="VFR25_08385"
     CDS             7467..8333
                     /locus_tag="VFR25_08385"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET9941108.1"
                     /translation="MTGRADEAVSSGRYAVLGDPVRHSLSPPMQNAAFRAAGIDARYE
                     ALGVPLERLDAVLAELHAGGVLGLNLTLPLKERAFELARTRTPEAERARAVNTLRREP
                     TGWAGHATDGLGFAAWIQELGIQLEGARVLLLGAGGAAASVAPIVLDHGPAAVAIASR
                     TPARAWSLAERLSGMGSVAIVAGGLEDEADIAREGPYDVLVRALTAEDVSEAEARLWR
                     ALEPRAPVLDLNYAERAALVRDRCKKEGRPFEDGLGLLLHQGALSFEFWTGRKAPLQA
                     MRDALRAAARSG"
     gene            8347..9105
                     /locus_tag="VFR25_08390"
     CDS             8347..9105
                     /locus_tag="VFR25_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941109.1"
                     /translation="MWKAWGRDLADAILDRRCPGCADRAPRYREVCDACDARIERTGI
                     ALCLACLRGDDPGPESSARGCPRHGSTRLALAGPPYDPTLERILRAFKYEGATRLASW
                     IADLVPEPPAAAWREMILVPVPLHPAKRATRGFDQALLLAELVSKRWGIPVVRALERI
                     REHPAQATLDAARRRVNVRGAFRCVDPRAVEGRPILLLDDVVTTGSTMLEAAAPLEGA
                     HAAWILGLSAARGGDPEAPEPKAAGAVAGETALW"
     gene            9210..>9644
                     /locus_tag="VFR25_08395"
     CDS             9210..>9644
                     /locus_tag="VFR25_08395"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET9941110.1"
                     /translation="MDKLRLLDEAYLPLVTKPSRYTGSFLHAARKDPSEARVRVLLCF
                     PDLYEIGMSYLGLKILYHIVNGRKDGAAELCFTPWGDMERLMRHEGIPLYSLESRTPA
                     RQFDMIGFSLQYELSYTNVLTMIDLAQIPLRTRDRREDDPILI"
BASE COUNT         1480 a         3294 c         3219 g         1651 t
ORIGIN      
        1 gagccgcgcc tacgaggcct acggacgggc ctatcccgac cgggatgggg cggccctcgc
       61 gctcttgaag tcggcggaga tcgaacagca cgccctcaac aaccccgggc gcgcgcgatt
      121 cctctacgac gagctcctgc gccggcccct cgggccggaa ctcgagcaga tggtccgcga
      181 gcgctgcgag ctcgccgaca ccgccctcgc gggctgaagc ctcccgctag gggaggcagt
      241 ccagctcgga cagctccttg gtgcggctcg cgggatcgaa gaggtaaacc cggaactgcc
      301 gccccggcgt caggggcgcc ctcggaatgc agcgcgagat gatcccgttc tccacgcttc
      361 tccggctctc gtggatgacg atctccttcg tcatgaggga gtgatcgggg gccgcgaacc
      421 agaggagcac ggtcgcgtcg gaagccggat cgtacggcgc gcgcagatcg tagacgccgt
      481 tcccgtccgt gatgcccgat accagctcct gcccctcgtt gaagaccttc acctgcacgc
      541 ccgagagcgg atggtcgttc aggtcgagga ccgttccggt gaacggcgtc agccgccgat
      601 cgagcccgag cgactggtac tcctcgatgc gcttcgttcc ggcaatcggg gactcgttgc
      661 tgcttccagg gggggaggcg gacgggggtg ctccctgaac gggccctgtc gcgaggagga
      721 agagggaaac gcttccaagg aacctcaaga acggacgcat catggccgat acctcccatt
      781 gcgtcgccgc ggagggattc cgaggcgacg ctacacccgg tcattcggcc tgtcaacgcg
      841 gcttccccag gagaatcgcc ttgcccattg ggggcctgtc tgttacggtc cggtcgcttc
      901 gcactaatcc accctccggc ccgcccggga cggggttagg agtcagccca ccatggccac
      961 gcagggattt cgggaggcac ggatgtttct cgaccaggtg atgggcgtct tctccaacga
     1021 cgtggcgatc gatctcggga cggcgaacac gctggtctac ctccggggaa agggcatcgt
     1081 cctcaacgag ccctcggtcg tcgccgtcga tcgaaccacc aataaagtca tcgccgtcgg
     1141 gaaggaagcg aagagcatgc tcggccgcac gcccgacgag atccacgcgg tgcgtccgct
     1201 caaggacggc gtcatcgccg atttcgagaa gacggaagat ctgctgcgcg agttcatcca
     1261 gaaggcgctc cggcgccgca cgtgggtgcg cccgcgcatc atcatctgcg ttccgtcggg
     1321 gatcaccgag gtggagaagc gcgcggtcca ggactcggcc cagcacgcgg gcgcacgcga
     1381 ggtgctgctc gtgcccgagc ccatcgccgc ggcgatcggc gtcggtctcc cggtcgggaa
     1441 gccctccggg aacatgatca tcgacatcgg cggcggcacg acggagatcg cggtcatggc
     1501 gctgaactcg atcgtgaacc agcagtcgat tcgcgtcggc ggcgacgaga tggacgaggc
     1561 catcgtccag tacgtgaaga aggcctacaa cctcctgatc ggcgagcaga cggccgagca
     1621 gatcaagatc cggatcggct ccgcgttccg gctcgagcag gaggaggaga tggagatcaa
     1681 ggggcgcgac ctcgtcgcgg gcatccccaa gaccatgaag atctcctcgg tcgaggtccg
     1741 tgaggccctc agcgagccgc tccagcagat cgtggacgcg ctcatgcagt ctctggagaa
     1801 gacgcccccc gagctcgcgt cggacatcgt ggaccggggg atcgtgatga cgggcggcgg
     1861 atcgctcctg cgcgggatcg acatgctgct tcgtgaggct accaacctgc ccatcaccgt
     1921 ggccgaggat ccgctctcgt gcgtcgtgct cggcacgggg aagatcctgg acgatccgac
     1981 ccagtacgag aaggtcctca tgtcggtggt gcgcgactga cgcaaggggc gtaagccctc
     2041 atgctccgct acctgttcgt acggcgcacc gactggacgg tgctggtcat cgcctgcacc
     2101 ctctcgctct cgatgatgct gctcgaggcg aggatgcagg ctcgggtcgc ttggttcctt
     2161 cagcacaccc tcctcgcgcc gttcgagtac tcgatcggct gggtcgacca gtcggtcggc
     2221 gtcttctggg agaaccacca gctccggagc cgtctgaccc agctccagat cgagtcggac
     2281 gccctccaag ccgagcgcat cgagaacgtc cggctccgcc gcctgctcca cctcgaagaa
     2341 cgccatccct acaacctcgt cgccgcgagc gtcgtcgcgc ggagcctcga ccgtctcggc
     2401 gggagcctca cgatcgacaa ggggacagcg gacggcgtcg agccgggacg cgccgtgctc
     2461 acgccggacg gcctggtggg gcgcgccgag cgctcgacac cgcatgagac gcgcgtgctc
     2521 acgctcttga accgcgattg cgctgtcgcg gcccgcatcg agcggacgcg cgtcgacggc
     2581 gtgctcgagt gggagttcgg aagccagccc gtgctcaatc tccggtacgt ctcgtcgcag
     2641 gaggacgtga aggtggggga ccgcgtggtc acctccggtc tgggcgggat cttcccggtc
     2701 gggatccgga tcggaacggt cgcgagggtc gcgctggatc ccaacggact catgaaggag
     2761 atcctcgtcg atcccgccgt cgacttccgc tccgtggagg agatcctcgt ctacacgccg
     2821 tccgatctcc gcggcacggc gccggcggat ctcttcctcg aagagccgaa gcccgcggcg
     2881 ccgggggact cgaccgcggc cgacagcgcc gcgacgcgtc cggccacccc atcgtcgaag
     2941 gcgatcgcca caacccagcc atcggagagc gcggctccgg agaaggtcgt ccggtgaagt
     3001 ggctccgtga cgggttcgtc ctcctggtcg ccctcgtgct ccagtcgacg gtcctccacc
     3061 ggatcgagat cgcgggggtt cgtcccgatc tcctcgtggc ctacgtcgtc tacattgcct
     3121 ggatgcgggg cccggtcgtg ggcacgatcg tgggctttgg cgtcggcctg atccaggacc
     3181 tcgacgcctc cgtgccgctc ggactgaacg cgctcgcgaa gtcactcgtg ggttttctcg
     3241 tcgcgaaggc cgggttccgg gtccatcgcg cgaacgccgg cgtgcgcttc gcgttcttct
     3301 tcatcgcgat gctcgtgcac gatgtcatct attatgcggt cgcgaccgac ggcgacctcg
     3361 ccatgttcgc gaagcagatg gtgctgagcg cggtcccgtc cgccctctac accggggtcg
     3421 tggtggtgat cctcctcggg atcgtcgagg ccttctcgag gaagctcctc ctggcggacg
     3481 aggtctgacg tggcctacga gatcgagccg cgccatcagg cgcgccggcg cgagcagatc
     3541 ttcacgatcg gcgtcgcgtc catcttcggc ctcctgatcc tccagctctt ctcgctccag
     3601 gtcctccagg gcgacaagta cggcgagctc agcgaggaga atcgaatccg cgtcgaggtg
     3661 ctcacggcgc cgcgcgggga gattcgtgac cgtaagggcc gcctgctcgc ggacaacgtg
     3721 ccctcgttca cggtcacgat cgatcccttc gacaaggtct acgtccacga tcccgcgctc
     3781 ctcgactcca cgatcgcgcg cgtgagccgc atcgtcgggc tcgaccccga ggtcgcgttc
     3841 gacaaggtga agcgggagcg gacccagtcg ttcctctcga tccgtctcaa gcgaaacctc
     3901 gacctcgcgt cggtcgcgta catcgcggag caccgggcgg agcttccggg cgtggaagtg
     3961 gaagcggagc ccctccggcg ctatccgctc ggtcccttcg cgtcgcacct cctcggctac
     4021 gtgggggagg tctcggacaa agagctcgag gatcccaagt tcgcgtcgac gtacatgcgc
     4081 ggcgatctga tcgggcgcat ggggatcgag cggcagtacg agaagatcct ccgcggtgtc
     4141 gacgggaagc gcttcgtgga ggtgaacgct ctcggccgga aggcggacct cttaggggac
     4201 aggcagccga tccttcccaa gcggggcgag gacatcacgc tctcgatcga tctcgatctc
     4261 cagcgcgcgg cggaggaggc gttccagccc ggtgcgcgcg gggcggtcgt cgcgatggat
     4321 ccgcgcacgg gagaggtgct cgctctcgtg agcaagccca actacgatcc gaacgaattc
     4381 tcgaccggga tcacccaggc acgctgggag gagctgagcc agggaggaaa ctatccgctc
     4441 ttcaatcgcg cgatccaggc cgcttacccg cccgggtcca cgctcaagcc gttcgtcgcg
     4501 ctggccggac tcacgcttcg cgccatcacg ccggtgacca cgttcgccga cccctgcttt
     4561 ggctactacc agttcggccg gcggcgcttc ggttgctggg accacgacgg gcacgggtgg
     4621 acgtcgctcc gctccgcggt ggccaagtcc tgtgacgtgt acttctatca gctcgggctc
     4681 aggatcgggc tcgatcgact ctcgacgttc atgaagcagt tcggcgtcgc cgaccggacc
     4741 ggcatcgatc tcccgcagga gcgccgaggt ctccttccga gcgcggcctg gtacgacaag
     4801 cactacggag ccggacgctg gagcaagggg ctcattctga acctcgcgat cggccaggga
     4861 gaatggtcac tcacaccggt gaagctggct cagatgacct cggtcctggc gagcggcggc
     4921 acgctttgga agcctcacat cctcagaagc gtcagccgcg acggagggga ggatcgaagc
     4981 gcgatcccgc gggcggacac cctctcgacg cacctcgact acccggcgcg ggacctcagc
     5041 tccgtccgtg acgcgatggt ggctgtcgtg gccgatggga ccgggtacca agcgaagatg
     5101 gattcgatca ccgttgctgg aaagaccggc accgcgcaga acccgcacgg gcaggaccac
     5161 gcgctcttca tctgctacgc acccgcatcc aggcccgaga tcgtggtggc ggtcctggtc
     5221 gagaacgcgg gccatgggag caccgcagcg gctccgctcg ctcagaaggt gctgcaggcc
     5281 tacttccacc cggcgcctcc cgacaccacc acggcgccca aggcccagac cgtggccctc
     5341 cggagcggca catgatcgcg tcgcgactca ccgcggacat cgaccgtccg ctcttcctta
     5401 cgacgatcct tctcgccttg atcgggatcg ccttcgtgtt cagcgcgacc gccatgcaga
     5461 cggcgcaggt cgagcacggc ctctacctga agcagttctt gtggctggga ctcgcgctcg
     5521 ccggcgcggc ggcgacggcc gcggtgccgt accgcatcta cgaggggaag acggcctggc
     5581 tcctctatgc gatcacgctc gtgctcctcg cgctcacgct ggtcatcggg cacgtgggtc
     5641 tgggagcgca gcgctggctc ggatggggac cgctcaagtt ccagccctcg gaggtcgcca
     5701 agatcgcggt cgtcctcgtg ctcgccagca tgctcgccgc gaagcggatc gacctcacgc
     5761 acctgccgac cctcttcaag acgctcctgg ccgccggcgt tccgtttctg ctcgtgctga
     5821 agcagcccga tctcggaacc tcgctctgct tcatcatggt cgtgctgacg atgctctact
     5881 gggctggcct tccgatgctc tacctgttcc tcctgctctc tccgatcctg aacggggtgc
     5941 tctcgttcta tctgccggcc tggatcctgt tcgcgctcgt gctcgcgttc gtgctctaca
     6001 ggtcgcggct ccggctctgg acggtcgtgc tggtcgtggt cgtgaacctc gccgtcggga
     6061 tcgcgacgcc gcatgtctgg aaccacctcg agccctacca gaagcagcgc atctccacgt
     6121 tcctcgaccc gagcgccgat ccctatggcg cgggctacca gatcatccag tcgaagatcg
     6181 cgatcggatc ggggggtgtg acggggaagg gcttcatgca cggcacgcag aaggggctcg
     6241 agttcctgcc cgaacagcac accgacttca tcttctcggt ggtgggggag gagaccggct
     6301 tcctgggggc tgcgttcgtg acggtgctct acatggtgtt catcctgcgc ggcgtgcgca
     6361 tcgcccggcg cgcccggaat cgctttggga gcctcctggc cattggattg acctcggttt
     6421 tcctatacca tgtgctcgtc aacgtgtgca tgaccgtcgg gctcgccccc gtgaccggcc
     6481 tccccttacc gcttctgagc tacggcggga cgtcgctctt gacgagcttc ctccagatcg
     6541 gattgatcca gaacgtcgcc atgcgatggc gcgaatactg acgtaggaga atcgtttgca
     6601 ccccactctg ttccacatcg gctccttcca gatccacacg tacggcctcc tcctcgcgat
     6661 cggattcctc atcgcgatcc agatcttcgt cgcccgcggc gtccgccgcg gcgtcgccga
     6721 ggaccagctc catacgatct ccctcgtcat cctgatcctg gcgatcgtcg gcggccgcgg
     6781 cctcttcgtg ctgacccact ggagcgacta cgcgaaggat tggctcggcg tgttccggct
     6841 gtgggaaggc ggcctcatgc tgtacggcgg ctacatcctc gcgatcgccg gcgggatctg
     6901 gtacgtccgg cgggcgaagc tcccggtgtg gcgcatcggc gatgcggcgg cgccggccat
     6961 ggcgctcggg atcggcattg gccggctcgg ctgctacttc aacggctgct gcttcggact
     7021 gccgacgcac cttccgtggg cggtgacctt cccgccgggg tcctacgcgt cgtacgcgtt
     7081 ccccggcgag gccatccatc cgtctcagat ctatctcgcg ctcgcgggcg tgtttctctt
     7141 cgggctcctc ctcgcgatgg accggaaagc ccacttcgac ggctggctct tctggaccgc
     7201 cgtggccatc gacgcggtgt tccggttcgg catcgacttc acgcgctact acgactcgac
     7261 gagctatctc gggcggctcg gtccgctcag cttcaacatc aaccagatcc tgagcgcgat
     7321 cctcttcctc accgcgctcg tcatgctgcg cgttctctcc cggcggccgg ctccgagcgc
     7381 ggccgcgacc ggcaccgagc cgggcgacga aaccgcggcg tcggcgctcg gcacccaggc
     7441 ggggtgaccg ggtccgcgct tcccggatga ccggacgcgc ggacgaagca gtctcgtccg
     7501 gccgctatgc ggtgctcgga gatcctgtcc ggcactcgct ctctcccccg atgcagaacg
     7561 cggcattccg ggccgcggga atcgacgcac gctacgaggc tctgggggtt ccgctcgagc
     7621 gtctcgatgc cgtgctggcc gagctccatg cgggcggcgt cctgggtctc aatctcacgc
     7681 tcccgctcaa ggagcgcgcg ttcgagctcg cgagaacgcg cacgcccgaa gccgaacgcg
     7741 cccgcgccgt gaacaccctc cgccgcgaac cgacggggtg ggccgggcac gcgacggacg
     7801 ggctcggatt cgctgcatgg atccaagagc tcgggatcca gcttgagggg gcgagggtgc
     7861 tccttctcgg cgcggggggg gccgccgcga gcgtcgcgcc gatcgtgctc gaccacgggc
     7921 cggcggcggt cgcgatcgcg agccggacgc cggcgcgcgc gtggtcgctg gcggagcggc
     7981 tgtctgggat ggggagcgtc gcgatcgtcg ccggcggact cgaggacgaa gcggacatcg
     8041 cgcgtgaagg accgtacgac gtcctcgtgc gcgcactcac ggccgaggac gtgagcgagg
     8101 cggaggctcg gttgtggcgt gcgctcgagc cgcgcgctcc ggtgctcgat ctcaactacg
     8161 cggagcgggc cgcgctcgtg cgggaccggt gcaagaagga agggcgaccg ttcgaggacg
     8221 gtctgggact ccttctccat caaggggcgc tgtcgttcga gttctggacc ggacggaagg
     8281 ctccgctcca ggcgatgagg gacgcgcttc gagcggcagc ccggagcggc tgacgggggt
     8341 agggggatgt ggaaggcgtg gggtcgggat ctcgcggacg cgattctcga ccgtcggtgt
     8401 cctggatgcg cggatcgcgc gccgcgatat cgtgaggtct gcgacgcgtg cgacgcgcgc
     8461 atcgagcgga cggggatcgc gctctgtctc gcttgcctgc gcggcgacga tcccgggccg
     8521 gaatcgagcg cgcgcggatg tccgcgccac ggatcgacgc gcctcgcgct ggccggcccc
     8581 ccatacgatc ccaccctcga gcgcatcctt cgcgcgttca agtacgaggg agccacccgg
     8641 ctcgcgtcct ggatcgcgga cctcgtgccg gagccgccgg cggccgcgtg gcgcgagatg
     8701 atcctcgttc ccgttccgct ccatcccgcg aagcgcgcca cgcgcggctt cgatcaggcg
     8761 cttctcctcg cggagctcgt atcgaagcgg tggggtattc cggtcgtgcg ggctctcgag
     8821 cggattcgcg agcaccccgc gcaggccacg ctcgacgccg cacgccgtcg cgtcaacgtc
     8881 cgcggggcct tccgatgcgt ggacccgcgc gccgtggagg ggcgcccgat tctcctcctg
     8941 gacgacgtcg tgaccaccgg gagtacgatg ctcgaggcgg ccgcgcctct cgaaggcgcc
     9001 catgcggcct ggatcctggg cctttccgcg gcccgcggag gcgatcccga agcgcccgaa
     9061 cccaaggcgg cgggagcggt tgcgggcgaa acggcgctat ggtaaggtcg caggcttcgc
     9121 gaggagcgcc cgcaaggtcc ctcggaagct cgtaaccggt tctcaacatt cgagatttgc
     9181 cctggtggcc cgtccacgag aggagatcaa tggacaagct gcgtctgttg gatgaagcgt
     9241 acctgccgct cgtgaccaag ccgagccggt acacggggag cttcctccac gccgcgcgca
     9301 aggatccgtc cgaggcccga gtccgggttc tcctctgctt cccggatctc tacgagatcg
     9361 ggatgtcgta cctcggcctg aagatcctgt accacatcgt gaacggccgc aaagacggcg
     9421 cggccgagct ctgcttcacg ccgtggggcg acatggagcg gctcatgcga cacgagggaa
     9481 tccccctcta ttcgctcgag tctcgcaccc ctgcgcgtca gttcgacatg atcggcttct
     9541 cgctccagta cgagctctcg tacacgaacg tgctcacgat gatcgacctg gcgcagatcc
     9601 cgctccgcac gcgggaccgg cgcgaggacg atcccatcct gatc
//
LOCUS       DASTKC010000200         4325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1581708, whole genome shotgun sequence.
ACCESSION   DASTKC010000200 DASTKC010000000
VERSION     DASTKC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581708"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFR25_08400"
     CDS             <1..818
                     /locus_tag="VFR25_08400"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941111.1"
                     /translation="RLHPTLGMAATRLVSRVAAGVLAPDGELLDVPRGGEVAFLAPLA
                     VRLLPSARERAVAARLDLLNARAVGDIQASALEPLRAAFGAAAAAALWREARGLDAAP
                     RRALPLPVAAAEETLAVETNDLRVLGARMARLVVELAGGLRSRRAEAASLAVEVGYAD
                     GREGRASGSLEPGRATGEAAPGTVAHLERTALALLTRAAARRVRVRRIRIEARERPPV
                     SRQLDFWLEARDRPTRRDQLDEALLALRARLGTAAVIPAAWMAHGLVRPSPRP"
     gene            787..3786
                     /locus_tag="VFR25_08405"
     CDS             787..3786
                     /locus_tag="VFR25_08405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9941112.1"
                     /translation="MGSFAHLHVHSHYSMMRGVDTLETLALAARERGMDRFALTDTNA
                     LYGFVFYRQICDEAGLKAIAGAEIVESAGTGNGNGHAPNSAILLARGREGYKSLCRVI
                     TERHLDPTFTIARSVRAHAAGLTLLSEDRAGLAALRDALPVYAELVPGRGDRALRQWA
                     RATDIPCVATNAVHFVRLEGHRIHQVLRAIDENTTLDRLDPAALAEPARSLLAPHEME
                     QRLPHAPEALENAVRLAHECTIDWPMGRTVFPSYPLDRGDAFAILSARCEAGILHRYG
                     KNPPNEVRTRLERELAIIRDKGFADYFLIVEAITSRTPRICGRGSGAASIVAYLLAIT
                     HVDPVRHNLFFERFLSPARTDPPDIDVDFAWDERDQIQLDVLAENGAPVRAAMVANHV
                     GFRGRGAIHEIAKVYGLPEAEIMNVTKRLAAFWGYEDEIEQMGNHPRFQDVDFEPPWP
                     EILALATALEGHPRHLAVHSGGIVLVPEALADHVPVEIAPKGVPIVQWEKDQVEDYGL
                     VKMDLLGNRSLAVIRDALAAVARNTGVTIDERHWNPVDDPETQRLMAEGRTVGVFYVE
                     SPSMRQLQRKAKVGDFDHLVIHSSMIRPASNKYIREYVRRLHGAPYDPLHPALRHTLD
                     ETYGILCYQEDVTKVAMELGGLSLADAEALRKALSHKRPVRPLRSYQAEFYEGARQRG
                     ASPDVIEKVWTMILSFAGYSFCKPHSASYALVSFRSGWIRAHHPAEFIAAVISNQGGY
                     YQTFAYVSEAKRMGLALLPPDVNASEREYTGKGREVRMGLMQLKGLHAESLEALLEER
                     NRSGPFGSFAELRGRVRLPPSDLELLVKSGALDSVAAGRSRPELLWELYLDGQAHRSQ
                     TLDLFAPEPVAAPPTPAYDRATMLRHEVETLGFLLSAHPLEPYERVMRARGVIPARDI
                     DRHAGRRVTVLGWYVQGKLVETYAHEAMEFIAFEDTTALYDVTLFPEAYRRFCHLLTE
                     RRPFLLTGLVEEDFGVCSLTVERVERL"
     gene            complement(3788..4303)
                     /locus_tag="VFR25_08410"
     CDS             complement(3788..4303)
                     /locus_tag="VFR25_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET9941113.1"
                     /translation="MKPAKIVGHHIHPMLIVFPLGLLAVSVFFDIAYLATHEAAFATI
                     AYWDMLAGIIGGLAAAVFGFWDWLTIQSGTRAKRIGAWHGLNMVVVVGLFAASWLIRR
                     TDAFYVPSSSALTLSFIAVVLALIGGWLGGELVERLGIAVSPDAGPNAPSSLHKDHGP
                     MREPAQGRTAR"
BASE COUNT          647 a         1560 c         1486 g          632 t
ORIGIN      
        1 tccgcctcca tcccacgctc ggaatggcgg ccacgaggct cgtgagccgc gtcgccgcgg
       61 gcgtgctcgc gcccgacggc gagctcctcg acgtgccgcg gggcggagag gtcgcgttcc
      121 tcgcgcctct cgccgtgcgc ctgcttccct cggcgcgcga gcgtgcggtg gcggcaaggc
      181 tcgatctctt gaatgcacgc gccgtcgggg acatccaggc gagcgcgctc gagccgctcc
      241 gcgccgcgtt cggcgccgcg gccgcggccg cgctctggcg cgaggcgcgc gggctggacg
      301 ccgcgccccg ccgcgcgctt cccctgcctg tcgccgcggc ggaagagacg ctcgccgtag
      361 agacgaacga tctcagggtg ctcggcgcgc gtatggcgcg gctcgtggtg gagcttgcgg
      421 gcgggcttcg atcgaggcgc gcggaggcgg cgtctctcgc ggtcgaggtg ggctatgcgg
      481 acgggcgcga ggggagggcg tcagggtcgc tcgagcccgg ccgcgccacc ggcgaagccg
      541 caccgggaac cgtggcgcac ctcgagcgca cggcgctcgc gctcctcacg cgcgcggcgg
      601 cccggcgcgt gcgcgtgagg cggatccgca tcgaagcgcg cgagcggccg cccgtgagcc
      661 gccagctcga cttctggctc gaggcacgcg accgcccgac tcgccgcgat cagctcgacg
      721 aggcgctcct cgcgctccgc gcgcgcctcg gcaccgccgc cgtgatcccc gcggcgtgga
      781 tggcccatgg gctcgttcgc ccatctccac gtccatagcc actactcgat gatgcgcggg
      841 gtcgacacgc tcgagaccct cgcgctcgcg gcgcgcgagc gcggcatgga ccgcttcgcg
      901 ctcaccgaca cgaacgcgct ctacggattc gtcttctacc ggcagatctg cgacgaggcc
      961 gggctcaagg cgatcgcggg ggcggagatc gtggagtccg ccggcacggg gaacggcaac
     1021 ggccatgcgc ccaacagcgc catcctgctc gcccgtgggc gcgaggggta caagtcgctc
     1081 tgccgcgtca tcaccgagcg ccacctggat cccacattca ccatcgcgcg ttcggtgcgc
     1141 gcgcatgccg cggggctcac cctcctctcc gaggatcgcg ccgggctcgc cgcgctccgc
     1201 gacgcgctcc ccgtctatgc cgagctcgtt cccggccgcg gcgatcgcgc gctccgccag
     1261 tgggcgcgtg ccacggacat cccgtgcgtc gcgacgaacg ccgtccactt cgtccgctta
     1321 gagggccacc gcatccacca ggtgctccgc gccatcgacg agaacaccac gctggaccgc
     1381 ctcgaccccg ccgcgctcgc ggagccggca cgctcgctcc tcgcccccca cgagatggaa
     1441 cagcgcctgc cgcacgcgcc cgaggccctc gagaacgcgg tccggctcgc gcacgagtgc
     1501 acgatcgact ggccgatggg gcgcacggtc tttccgtcgt acccgctcga ccggggcgat
     1561 gccttcgcga tcctctccgc gcgctgcgag gcggggatcc tccatcggta cgggaagaac
     1621 ccgcctaacg aagtgcggac gcggctcgag cgcgagctcg cgatcatccg ggacaaggga
     1681 ttcgccgact acttcctcat cgtggaagcg atcacgtcgc ggacgccgcg catctgcggc
     1741 cgcgggtcgg gggcggcgag catcgtggcg tatctcctcg cgatcaccca cgtcgatccc
     1801 gtccggcaca acctcttctt cgagcgattc ctctcgccgg cgcgcacgga tccgcccgat
     1861 atcgacgtcg atttcgcctg ggacgagcgg gatcagatcc agctcgacgt gctcgcggag
     1921 aacggcgccc ccgtgcgggc ggcgatggtc gcgaatcacg tcggcttccg agggcgcggc
     1981 gcgatccacg agatcgcgaa ggtctatggg ctgcccgagg cggagatcat gaacgtcacg
     2041 aagcggctcg ccgcgttctg gggctacgag gacgagatcg agcagatggg gaatcacccg
     2101 cgcttccagg acgtcgattt cgagccgccg tggccggaga tcctcgcgct cgcgaccgcg
     2161 ctggagggac atccgcgcca cctcgccgtc cactccggcg gcatcgtgct cgtgcccgaa
     2221 gcgctcgccg atcacgtgcc ggtcgagatc gcgccgaagg gcgttccgat cgtgcagtgg
     2281 gagaaggacc aggtcgagga ctacggcctc gtgaagatgg acctcctcgg caaccgatcg
     2341 ctggccgtga tccgggatgc gctcgccgcc gtcgccagga acacgggcgt cacgatcgac
     2401 gagcgccact ggaaccccgt cgacgatccc gagacgcagc ggctgatggc ggaagggcgc
     2461 acggtcggcg tcttctacgt cgagtcgccg tcgatgcgcc agctccagcg gaaggccaag
     2521 gtgggcgact tcgaccacct cgtcatccac tcgtccatga tccgccccgc gtcgaacaag
     2581 tacatccgcg agtacgtccg ccggctgcac ggcgcgccgt acgatccgct ccatcctgcg
     2641 ctccgccaca cgctcgacga gacgtacggg atcctctgct accaggagga cgtgacgaag
     2701 gtcgcgatgg agctgggcgg gctctcgctc gcggacgcgg aggcgctgcg caaggcgctg
     2761 agccacaagc gcccggtgcg gccgctccgc tcgtaccagg cggagttcta cgagggcgca
     2821 cggcagcgcg gcgcgtcgcc ggacgtgatc gagaaggtgt ggacgatgat cctctccttc
     2881 gcgggctact ccttctgcaa gccgcacagc gcgtcctacg cgctcgtctc cttccggagc
     2941 ggatggattc gcgcgcacca tcccgccgag ttcatcgcgg ccgtgatctc gaaccagggg
     3001 ggctactacc agacgttcgc ctacgtctcc gaggcgaagc gcatggggct cgcgctgctt
     3061 ccgcccgacg tgaacgcgag cgagcgcgag tacacgggga agggccgcga agtccgcatg
     3121 gggctcatgc agctcaaggg gctccacgcg gagtcgctcg aagcgctgct cgaggaacga
     3181 aaccggagcg ggccgttcgg gtcgttcgcc gagctccgcg gccgcgtcag gctgccgccg
     3241 tccgacctcg agctcctcgt gaagtcgggt gcgctcgact ccgtggccgc cgggcgctcg
     3301 cggccggagc tcctatggga gctctatctc gacgggcagg cccatcgctc gcagaccctc
     3361 gacctcttcg cccctgagcc cgtcgccgcg ccgccgacgc ccgcctacga tcgcgcgacg
     3421 atgctccgcc acgaagtgga gacgctcggc ttccttctga gcgcgcaccc gctcgagccg
     3481 tacgagcgcg tgatgcgggc acgcggcgtg attcccgcgc gcgacatcga ccggcatgcg
     3541 gggcgccgcg tcaccgtgct cggctggtac gtgcagggaa agctggtcga gacctatgcg
     3601 cacgaagcga tggagttcat cgcattcgag gacacgacgg cgctctacga cgtcaccctg
     3661 ttcccggagg cgtaccgccg cttctgtcat ctgctgaccg agcggcggcc gtttctcctc
     3721 acgggtctcg tcgaagagga cttcggagtc tgctcgctca ccgtggagcg ggtcgagcgg
     3781 ctgtagccta ccgggccgtc cgtccctggg cgggctcccg catgggaccg tgatccttgt
     3841 gcagcgagct cggagcgttc ggccccgcgt ccggcgacac ggcgatgccc agccgctcca
     3901 ccagctcgcc tccaagccat ccgccgatca gcgcgagcac gaccgcgatg aacgagaggg
     3961 tgagcgcgga cgacgacgga acgtagaagg cgtccgtccg gcggatgagc cagctcgctg
     4021 cgaagaggcc gaccacgacg accatgttga ggccatgcca cgcgccgatg cgcttggcgc
     4081 gcgttcccga ctggatggtg agccagtccc agaaaccgaa caccgcggcc gcgaggccgc
     4141 caatgatgcc ggcgagcatg tcccagtacg cgatggtcgc gaacgcggct tcatgcgtcg
     4201 cgagatatgc gatgtcgaag aagacagaga cagcgagaag tcccaagggg aatacgatca
     4261 gcatggggtg aatgtggtgc cctacgatct tcgcgggttt catcgacaac ctccttccct
     4321 caacc
//
LOCUS       DASTKC010000201         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_399166, whole genome shotgun sequence.
ACCESSION   DASTKC010000201 DASTKC010000000
VERSION     DASTKC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399166"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VFR25_08415"
     CDS             complement(<1..1281)
                     /locus_tag="VFR25_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET9941114.1"
                     /translation="MRPSWPHFVWPAPVVLWFLIALGHLASTWATVPARIEEPALVAA
                     LACGVLSLGIGGRPHPYRRALWSWLEAALGAASLIAAGVTLGAEADSLAWPLPVHPAE
                     TPVVIEGRVLDTTATDAEFPSLVVEARRVRVGSREAACRAKVTVRWRDDAIPPRWVLP
                     GLWLRLRGDFRPPEDARNPGSSAPGRALERLGLAGTVTIDPLSIEAPADPPERGSDPG
                     AIIRDRLARAFAASLSHPVAALARGMLLGDRSGIAPAIQHSFRDGGTVHILSISGLHV
                     CILAGFIALFAGMLRLPPAPTAAIELAVLWGYVALVGAPASALRSALLWTSARCGRLL
                     GVVVRPFTAWGIAGLALHLADPRSVLDPGFQLSFLAVLGLGAAGPLGGLVKAGASGGG
                     HRGLARRTAAGGWSLFVQSGGATIGTAGVQSTMFG"
     gene            complement(1339..>3404)
                     /locus_tag="VFR25_08420"
     CDS             complement(1339..>3404)
                     /locus_tag="VFR25_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9941115.1"
                     /translation="DPDRGFALVARGAMGPADVIDPTIWVKEKLALALSTTLDAEETP
                     LKRVIGALRALHAELMARPEGDRPWVAVLALLLHGGDAVAVSAGDCPCFRHRSGLLSR
                     LGRAEPDSGHSAPRGALGSEPQVRIEVVPLKPEPGDVYVLSTRPLRDGEIALLARDLA
                     GAMNPVQVLRAASDGAADRGRVAVRVLQPQEPESIGPEAEQAAEMHDVVTAVAETEPV
                     EPLDVAGFATEAPPAFMGEHAAADVIEEAPGHGVLDPIAPAIADSAPSVASGAPGFVV
                     EAIDDAAREGTEASVETEESVASSDSDRPRTPTLAPIGEERAWYEPLALWGGGALAII
                     AVAVLIRALLPGILGDSSRRSSAPPMPAAVSGTADIFSDPPGAVVHVDGVALEGRTPL
                     TGVSLDAGLHKVELDWGPYGAWRDTVEISAATRLTLHPALYGTVAITSSEPKRVLDVY
                     LDGVYMGTTPITLDQVVVGRHLVRFSGPGIASSTQGIDVLKDKSAELVGSVGPTPLDG
                     SVEVKSTILGDAGFEPSKGDPFWVDGVPRGVTPGKITLGPGTHSVRVARRGFPPQVSI
                     LDVKSGGQAFVTAEFGEHSDEPLRFDAPDAVDRSNPLPLTITLPESEWDPSLALWLYA
                     APPGGTFQPKRMTRLEEGSRSYAALVPPEVLRSKVGQVRIYFMASGTSGHEIYSEIYT
                     LPVRN"
BASE COUNT          540 a         1233 c         1219 g          412 t
ORIGIN      
        1 gccgaacatc gtcgactgga cgcccgcggt tccgatcgtg gccccgccgc tctgcacgaa
       61 gagggaccag cctcccgctg cggtgcgccg cgcgaggccg cgatgccctc cgccggaggc
      121 acccgccttc accaggccgc cgagcggtcc ggccgcgccc agcccgagca ccgcgaggaa
      181 cgagagctga aatccgggat cgagcaccga tcgcggatcg gcgaggtgga gcgcgagccc
      241 cgcgatgccc cacgcggtga agggacgcac gacgaccccg agcaagcgcc cgcatcgcgc
      301 ggacgtccag aggagcgcgg agcggagcgc cgatgccggc gcgcccacga gcgcgacgta
      361 gccccagagc accgccagct cgatcgccgc ggtcggggcc ggcgggagcc ggagcatccc
      421 tgcgaagaga gcgatgaagc ccgcgagaat gcagacgtgg agtcccgaaa tcgagaggat
      481 gtggaccgtg ccgccgtcgc gaaacgagtg ctggatcgcg ggcgcgatgc cggagcggtc
      541 ccccaggagc atccctcgcg cgagcgccgc gacgggatgg gagagcgacg cggcgaacgc
      601 acgcgcgagg cggtcgcgga tgatcgcgcc cggatcggag ccgcgctccg gcggatccgc
      661 gggcgcctcg atcgagagcg gatcgatcgt gacggtgccg gcgaggccga gccgctcgag
      721 ggcgcgccca ggcgcgctcg agcccgggtt gcgcgcgtct tccgggggac gaaaatcgcc
      781 gcgcaaccgg agccacaacc ccggcaacac ccaccgcggc gggatcgcgt cgtcccgcca
      841 gcggacggtg accttcgcgc ggcacgcggc ctcgcggctc cccacgcgca cgcggcgggc
      901 ttccacgacg aggctcggaa actccgcgtc ggtcgccgtc gtgtcgagca cgcgcccctc
      961 gatcaccacg ggcgtttccg cgggatgcac ggggagcggc catgcgagcg aatcggcctc
     1021 cgccccgagc gtcacgccgg ccgcgatcaa ggaagccgcg ccgagagccg cttcgagcca
     1081 cgaccacagg gcgcgccggt acggatgagg gcgccccccg atcccgagcg acaacacgcc
     1141 gcacgcgagc gcggccacga gcgcgggctc ttcgatgcgc gccggcaccg tcgcccatgt
     1201 cgaagccaga tgtcccagcg cgatcaggaa ccacagaacg accggcgcag gccagacgaa
     1261 gtgaggccag gagggacgca tcgcgtcctc gctgcgggga ggtccatcga gcgcggcgag
     1321 gaggggccgc gcgtacagct agttccggac ggggagtgtg tagatctcgg agtagatctc
     1381 gtgtccgctc gtgccggacg ccatgaaata gatgcgcacc tgacccacct tggaccggag
     1441 cacctcgggg ggcacgagtg ccgcgtagga gcgcgagccc tcttcgaggc gcgtcattcg
     1501 cttcggctgg aacgtgccgc ccgggggcgc cgcgtagagc cagagcgcga gcgacggatc
     1561 ccattcggac tcgggaagcg tgatggtgag cgggagcgga ttcgaccggt cgaccgcgtc
     1621 gggcgcgtcg aaccggagcg gctcatcgga atgctcgccg aactccgccg tgacgaacgc
     1681 ctgcccgccc gacttcacgt cgaggatcga gacctgcggc gggaaaccgc gccgcgccac
     1741 gcgcacgctg tgcgtccccg gtccgagcgt gatcttgccc ggtgtcacgc cgcgcggaac
     1801 gccgtcgacc cagaagggat cgcccttgct cggctcgaac cccgcgtcgc ccaggatcgt
     1861 gctcttcact tccacggagc cgtccagcgg cgtggggccg acgctcccga cgagctccgc
     1921 gctcttgtct ttcagaacgt cgatcccctg cgtgctcgag gcgatgcccg gaccgctgaa
     1981 ccgcacgaga tgccgcccca cgaccacctg gtcgagcgtg atcggcgtcg ttcccatgta
     2041 gacgccgtcg agatagacgt cgagcacgcg cttcggttcc gaagaggtga tcgcgaccgt
     2101 gccgtagagc gcggggtgga gcgtcagacg cgtcgcggcg gaaatctcga ccgtgtcgcg
     2161 ccacgcgccg tacgggcccc agtcgagctc caccttgtga agtcccgcgt cgagcgagac
     2221 gccggtcagc ggcgtgcgtc cttcgagcgc cacgccgtcc acgtgcacga cggcgccggg
     2281 cggatcggag aagatgtcgg ccgtgccgct caccgccgcg ggcatgggag gcgccgagga
     2341 gcgcctcgac gaatcgccca ggattccggg aagaagcgca cggatcagga cggcgaccgc
     2401 gatgatcgcg agcgcgcctc cgccccacag cgcgagcggc tcgtaccacg cgcgctcctc
     2461 gccgatcgga gcgagcgtcg gcgtgcgagg acggtccgag tcgctcgatg cgacggactc
     2521 ctcggtttca acggatgcct cggttccctc cctcgctgcg tcgtcgatcg cctcgacgac
     2581 gaagccaggg gcgccgctcg ccacggaagg agcgctgtcg gcgatcgcgg gagcgatggg
     2641 atcgagcacg ccgtgacccg gggcctcctc gatcacgtcg gcagccgcgt gctcccccat
     2701 gaaagccggg ggcgcttccg tcgcgaaacc ggccacgtcg agtggctcga ccggttcggt
     2761 ctccgcgacc gcggtcacca cgtcgtgcat ctccgctgcc tgctcggcct cgggaccgat
     2821 cgattcgggc tcctgaggct ggaggacgcg cacggcgacc cggccgcggt cggccgcacc
     2881 gtccgacgcc gctcgcagga cctggaccgg gttcatcgcc cccgcgaggt ctcgcgccag
     2941 gagcgcgatc tccccgtctc ggagcgggcg ggtcgagagc acatagacgt cgcccggctc
     3001 gggcttcagg ggcacgacct cgatccggac ctgcggctcc gatcccagcg ccccgcgcgg
     3061 cgcgctgtgc cccgaatcgg gctctgcgcg ccccaagcgc gagaggaggc cgctccgatg
     3121 ccggaaacag ggacaatccc ctgcgctcac ggccaccgcg tccccgccgt ggagaagaag
     3181 agcgagaacg gccacccagg gccgatctcc ctcgggccgg gccatgagct cggcatggag
     3241 cgcgcggagc gcaccgatga ctcgcttcaa gggcgtctcc tcggcgtcca gcgtggtgct
     3301 gagggccagc gcgagcttct ccttgaccca gatggtcggg tcgatgacgt ccgcgggccc
     3361 catggcccca cgcgccacca aagcgaagcc ccgatcgggg tcga
//
LOCUS       DASTKC010000202         6155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1818266, whole genome shotgun sequence.
ACCESSION   DASTKC010000202 DASTKC010000000
VERSION     DASTKC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818266"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..1063
                     /locus_tag="VFR25_08425"
     CDS             302..1063
                     /locus_tag="VFR25_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522151.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET9941116.1"
                     /translation="MDDERSRPALVGPGLMVLALGMVLAGWLVGNGIQSVRTADRFVS
                     VKGVAEREVKADLAIWPIQFAVTDDNIPAAQARVTANVIKIASFLAENGIDTSEVELT
                     SLRVTDVLANPYNPPNRVGNRYIIEQTVLVRTTRPDLVQAASRRVGELVNAGIPLSSG
                     QYGATGPTYVFRKLNDLKPSMIAEATAEARKAAEQFAKDSKSRLGGIHRANQGVFVIL
                     PRDASDSGGGPGMGEQSQMFKLVRVVTTVEYLLRG"
     gene            complement(1073..1300)
                     /locus_tag="VFR25_08430"
     CDS             complement(1073..1300)
                     /locus_tag="VFR25_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941117.1"
                     /translation="MRPLSILGVALIVLGLVALLAGGFSYTKDKETADLGPVDITVKD
                     KEHVHVPTPVSAGVIVLGVVLLFAGRRPSTA"
     gene            1613..3220
                     /locus_tag="VFR25_08435"
     CDS             1613..3220
                     /locus_tag="VFR25_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HET9941118.1"
                     /translation="MKSLRKAIVLVAATSAAFLAAPKHASSEVGGVHLNVTPFVGYGD
                     WAKDINLDSRAYFGGRLGVGIGPYLGVEGYYGWMGTHTEYGTGDSLFFQEGLSTSPRN
                     TTDLQLYGADLVLNLIPKSKFNPYLLGGWHEEKITPDSPGPRSFQNGAEFGGGVKIGV
                     SRRAAVRLELRDKLWSFGDSPTIPNPPGNDNIHNLFYNAGLQVSFGGATGSDADNDGV
                     ADSKDACPNTPAGALVDERGCPLDADGDNVPDGIDQCPNTPSGATVDARGCPSDADND
                     GVPDGIDTCPGTPGGTQVDAKGCPADQDGDGVPDGVDQCANTPTGARVDARGCPVDSD
                     QDGVPDGIDQCPNTLPGAQVDARGCAADSDNDGVTDDKDLCPNTQADVKVDAQGCPIE
                     LTEMETQLLDKGVITTREIHFETAKWDILPESRPILDGIGKVLIQWPKLHIEVGGHAD
                     ARGSDAYNMDLTQKRAQSVLDYLITNFPQITRDQYTAKGYGERKPVATNRTVEGMARN
                     RRVEFKVLNTEELTKERERRRLLKRGE"
     gene            3295..3558
                     /locus_tag="VFR25_08440"
     CDS             3295..3558
                     /locus_tag="VFR25_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941119.1"
                     /translation="MLTKLWHRTIVSLATLPVLVTSALAQDGGTGTTEGGTSATTTTT
                     TTTTSEWITDWRLWAVVGAVLVVILILALTRGRSEGGSTTIVK"
     gene            3998..4732
                     /locus_tag="VFR25_08445"
     CDS             3998..4732
                     /locus_tag="VFR25_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941120.1"
                     /translation="MKRSLLIPAFVVMALAGWELAYAQAPDNSGAAPGTPGTTTSTTT
                     TTTTTPSTTPPTDATPSTTSTTTTTEATTAQAPPASAAYLAARERGKGVTAKETATVD
                     KQLTTVTKQVDDLAAKAGEATVAGRLSSDFGVSSDALMAEKAKYNTGWGDLMIAHTLA
                     SNTLTGVTLDEVMTMRTTDAMGWGSIASGLDLKLGNVVSGVKSEAKVAMGSSRADGKP
                     AKIEVAAVTPPAAAGTAGTSGDTGTK"
     gene            4831..5781
                     /locus_tag="VFR25_08450"
     CDS             4831..5781
                     /locus_tag="VFR25_08450"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9941121.1"
                     /translation="MNHPDPHAQPGRNGRPTALVTGASGFVGGRVVKRLLEQGIPVRV
                     IVRETAEPPLDERIEVVRGDFVDPDTARRGVDGVFDVIHCAATAGPEIAPVRRVNVDG
                     TRALVDAALGQQVRRFIQISTISVYDREGRSAIDEDSPLKTSGDPYGLTKAEADRVVL
                     DAMKRGLQAVILRPGAILGAGETSTWATRVPQMVQEGKVKLRKDGTDTIPFIHVEDLV
                     DAVMLALHSDRAVGRVYDVADEHHTWREYTDEVRRWFDLPELDRVPDAEIQPGTFWIG
                     AVSTQRIRRELGFAPTRSYRDGMAEAEHAWRERRATTRRD"
     gene            5759..>6155
                     /locus_tag="VFR25_08455"
     CDS             5759..>6155
                     /locus_tag="VFR25_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9941122.1"
                     /translation="MPRPDATEFNPYYGRYIEKVPDGPIVDVLTSGMKETLTLLRSIP
                     ESRADHRYAEGKWSIKEVVGHVIDGERVFTYRALRFARKDTTPLASFEQDDYIPAGNF
                     GARTLRDLTDEYEAVRRATIELFRHFDETQ"
BASE COUNT         1146 a         2051 c         2037 g          921 t
ORIGIN      
        1 caccgcccca tttccggaga cggcctaccc ggctttctca gacttctcgc ttctcgcggt
       61 agatctcaac cgtcccatcg gccacatcgc catgcttatt taccaggtgg gggcggtcag
      121 caacggcttc cgcaggacgg cctggtcggt ggctccccgg atgaggcccc gattacggtt
      181 agagggaggg acgacggtcc gggggaagag ggacccgccc acccaggatc gcccaagacc
      241 caggtcctgc gagcgagttc gattgaacgg gatggttgcg ccagagtagg atcgccgccg
      301 catggacgac gaacgatcgc gtcccgccct cgtcggtccg ggactcatgg tgctcgcctt
      361 ggggatggtc cttgccggct ggctggtcgg caatgggatc cagagcgtcc gcaccgccga
      421 ccggttcgtc agcgtgaagg gcgtggccga gcgtgaggtg aaggccgatc tcgcgatctg
      481 gccgatccag ttcgcggtca cggacgacaa cattcccgcc gctcaggcgc gggtgacggc
      541 gaacgtcatc aagatcgcgt ccttcctcgc ggagaacgga atcgacacga gcgaggtgga
      601 gctgacgagc cttcgcgtga ccgacgttct cgcgaatccc tacaaccctc cgaatcgcgt
      661 cggcaatcgc tacatcatcg agcagaccgt gctcgtgcgg acgacgcgcc ccgatctggt
      721 gcaggccgcg agccggcgcg tgggggagct cgtcaacgcc ggcatcccgc tctcgtccgg
      781 ccagtacggc gcgaccgggc cgacctatgt gttccgcaag ctcaacgatc tcaagccgag
      841 catgatcgcg gaggcgaccg cggaagcgcg caaggcggcg gagcagttcg cgaaggattc
      901 gaagagccgg ctcggtggaa tccatcgcgc caaccagggc gtcttcgtca tccttccgcg
      961 cgacgcgtcg gattccggcg gcggtccggg catgggcgag cagagccaga tgttcaagct
     1021 cgtccgcgtc gtgaccaccg tggaatacct cctccgcggc tgaccactcg cgctacgcgg
     1081 tactcggtct cctccccgca aacaggagta ccacgccgag cacgatgacg cccgcgctga
     1141 ccggcgtggg aacgtgcacg tgctccttgt ccttgaccgt gatgtccacc ggcccgaggt
     1201 ccgcagtttc cttgtccttc gtatagctga agcccccagc gaggagcgcg accaaaccga
     1261 gcacgatcag cgcgacgccg agtatggaaa gcggtctcat gcttcctcct gtcgtatgcg
     1321 gcgctcgatc gcgccgacag gtccccgcca cgcaagagcc aagccaaaga cgcgcccgct
     1381 acggggaggc agatcgttcc acctccatcc tttgaaattc ctacacggct attgaaagag
     1441 acgctccggc cggccggctt cggcgcagac accgtccaac cggggaagca ggctctcggc
     1501 atgtaagtag gtggcctcgc tcacgatcct ccttcgtggc acagaccgtg ctcaaggctg
     1561 gcgcggattg cgttcatccg agcacgcttc catcccaccc cccggaggaa tgatgaagtc
     1621 actgcggaag gcgatcgtcc tggtggccgc gacgtcggcg gctttcctcg ccgcgccaaa
     1681 gcacgcgtcc agcgaagtgg gcggagtaca cctcaacgtc acacccttcg tgggttacgg
     1741 cgactgggcg aaggacatca atctcgacag ccgcgcgtat ttcggcggcc ggctcggcgt
     1801 cggcatcggc ccctatctcg gggtcgaggg ctactacggc tggatgggca cgcacacgga
     1861 gtacggcacc ggggactcgc tcttcttcca ggaaggcttg agcacgtcgc cgcggaacac
     1921 gaccgacctg cagctgtacg gtgcggatct cgtcctgaat ctcattccca agtcgaagtt
     1981 caacccctac ctcctcggtg ggtggcacga agagaaaatc acccccgaca gtcccgggcc
     2041 taggagtttc cagaacggcg ctgagttcgg tggaggcgtc aagatcggcg tgagccgccg
     2101 cgccgctgta cggttggagc tccgcgacaa gctctggtcc ttcggcgaca gtccaaccat
     2161 tcccaatccg ccaggaaacg acaacatcca caacctcttc tacaacgcag ggcttcaggt
     2221 ctccttcggc ggtgcgacgg gaagcgacgc ggacaacgat ggcgtggccg atagcaagga
     2281 tgcgtgtccc aacacgccgg ccggcgctct ggtcgacgag cgcggctgcc cgctcgatgc
     2341 cgacggagac aacgttccgg acggcatcga ccagtgtccg aacacgccct cgggcgcgac
     2401 cgtggatgcg cgcggctgtc cgagcgacgc cgacaacgac ggcgttcccg acggaatcga
     2461 cacgtgcccc ggaacgccgg gaggcacgca ggtggacgcg aagggatgcc ccgcggatca
     2521 ggatggtgac ggcgttccgg acggcgtcga ccagtgcgcc aacaccccga cgggagcgcg
     2581 cgtggatgcg cgcggctgcc ccgtcgatag cgatcaggac ggggttccgg acggcatcga
     2641 ccagtgcccg aacacgctgc cgggggctca ggtggacgcg cgtggatgcg cggccgactc
     2701 ggacaacgac ggcgtgaccg acgacaagga tctgtgcccg aacacgcagg ccgacgtgaa
     2761 ggtggacgcg cagggctgcc cgatcgagct gaccgagatg gagacgcagc tgctcgacaa
     2821 gggcgtgatc acgacccgcg agatccactt cgagacggcc aagtgggaca tccttcccga
     2881 gtcgcggccc atcctggacg gcatcggcaa ggtgctgatc cagtggccga agctgcacat
     2941 cgaggttggc ggccatgccg acgcgcgtgg ctcggacgcc tacaacatgg acctcaccca
     3001 gaagcgggcg cagtccgtgc tggactatct catcacgaat ttcccgcaga tcacccgcga
     3061 ccagtacacg gccaaggggt acggggagcg gaagccggtt gcgacgaacc ggacggtcga
     3121 gggaatggcg cggaatcgcc gcgtcgagtt caaggtcctc aacaccgagg agctcaccaa
     3181 ggaacgggag cgccgtcggc tcttgaaacg gggggagtag agagcctgca tccgcttccg
     3241 tagtgcaggt ggggaaccca aggttcccca cctgccacat tccaaggaga cgatatgctg
     3301 accaaattat ggcatcgcac gatagtgagt ctcgccacgc tgcccgttct cgtcacatca
     3361 gcgctcgcac aggacggggg cacgggaacg acggagggcg ggacctccgc cacgaccacc
     3421 acgaccacga cgaccacgtc cgaatggatc acggactgga gactctgggc cgtcgtaggc
     3481 gcggtgctcg tggtcatcct catcctcgct ttgacgcgcg ggcgcagtga agggggcagc
     3541 acgaccatcg tgaagtagcg cacgcggtgg cccttgtagg tttgcatcgg gagagccggc
     3601 gcctcgggcg ccggctcttc gtcatgagtg ggcgccgttg ttacaaggat gcgcggagtc
     3661 tgttccgatg cgtacagggc tggcgggccg cggggacgcc tacgcggaac ggtcttcggc
     3721 gcaagtccag cgtgagcacc gtccagcctg gattcacgcg catttctcct cagtggaacg
     3781 ggtgttgctt gagtgtcccc tagtcttctc aacgcctagg gcggcgggaa cctggttcaa
     3841 ttcgagctcg ctcttgacaa tcacaggttg gcgctggcaa tagcgaatcg atcgaacaat
     3901 aggaacccgc cgcccgcttt ggctgtactc ctccgcacag ggggtcgtac ccgcacacgc
     3961 cccgccacgg ggcactcaag gaaggaagga acagctcatg aaacgatcgc tcctcattcc
     4021 ggcgttcgtc gtgatggcgc ttgccgggtg ggaactcgcg tatgcgcaag ccccggacaa
     4081 ttcgggcgcg gctcccggca cgcccgggac gacgacgagc accaccacga cgacgaccac
     4141 aacgccgagc acgactccgc ccacggatgc caccccgagc accacgagca caacgaccac
     4201 gacggaagca acgaccgcgc aggctccgcc agcgagcgcc gcgtatctcg cggcgcgcga
     4261 gcgcggcaag ggcgtgaccg cgaaggagac cgcgaccgta gacaagcagc tcacgaccgt
     4321 gaccaagcag gtggatgacc tggccgcgaa ggcgggtgag gccaccgtgg cgggacggct
     4381 ctcgtccgac ttcggcgtct cgtccgacgc actcatggcg gagaaggcca agtacaacac
     4441 cggctggggc gatctcatga tcgcgcacac gctcgcctcg aacacgctga ccggcgtcac
     4501 gctcgacgag gtcatgacca tgcgcacgac cgacgcgatg ggttgggggt cgatcgcgag
     4561 cggtctcgac ctcaagctcg ggaacgtcgt gagcggggtc aagagcgagg ccaaggtggc
     4621 gatgggctcc tcgcgcgccg acggcaagcc ggcgaagatc gaggttgcgg cagtgactcc
     4681 gccggcagcg gcagggacgg ccggcacgag cggcgacacg ggcaccaagt agtcgagcgc
     4741 gcctgggacg ggcgcttagg gacaaaagca tcatttgagg cgagaggccg ccggaagcac
     4801 ggcggcctct ccgtctgcta tcctccctgc gtgaaccatc ctgacccaca cgcacagccg
     4861 ggccggaacg gacgccccac ggcgctcgtg accggagcgt ccggcttcgt cggcgggcgc
     4921 gtcgtgaagc gcctcctcga gcagggcatt cccgtgcgcg tcatcgtgcg cgagaccgcg
     4981 gagccgcccc tggacgagcg gatcgaggtc gttcgcgggg atttcgtcga cccggacacg
     5041 gccaggcgag gagtggacgg cgtgttcgac gtgatccact gcgcggccac cgccggtccc
     5101 gagatcgcgc cggtgcgccg cgtgaacgtg gacggaactc gggcactcgt ggacgccgcg
     5161 ctcgggcagc aagtccggcg gttcatccag atctcgacga tctccgtcta cgatcgcgag
     5221 gggcggagcg ccatcgacga ggactcgccg ctcaagacct cgggcgatcc ctacggcctc
     5281 acgaaagccg aagccgaccg cgtggtcctc gacgccatga agcggggcct ccaagcggtg
     5341 atcctccgcc ccggcgcgat cctcggagcc ggcgagacat ccacgtgggc cacgcgcgtg
     5401 ccgcagatgg tgcaggaggg caaggtcaag ctccgcaagg atggcaccga cacgatcccg
     5461 ttcatccatg tcgaagatct cgtcgacgcg gtgatgcttg cgctccattc ggaccgcgcg
     5521 gtcggccggg tctacgacgt cgccgacgag caccacacgt ggcgcgagta cacggacgag
     5581 gtccgccgct ggttcgacct gccggagctc gaccgcgttc ctgacgccga gatccagccc
     5641 ggcacgttct ggatcggagc cgtctccacc caacgaatcc gtcgcgagct gggctttgct
     5701 cccacccgct cgtatcgcga cggcatggcc gaagcggagc acgcctggag ggagcgccgt
     5761 gccacgaccc gacgcgactg agttcaatcc gtactatggc cggtacatcg agaaggtgcc
     5821 ggacggcccg atcgtcgacg tgctcacctc cggcatgaag gaaacgctga ccctcctccg
     5881 ctcgatcccc gagtcccgcg ccgaccaccg ctacgccgag gggaaatgga gcatcaagga
     5941 ggtcgtgggg cacgtcatcg acggagagcg cgtcttcacc taccgggcgc ttcgcttcgc
     6001 acggaaggac acgacgccgc tcgcgagctt cgagcaggac gactacattc ccgcggggaa
     6061 cttcggggcg cgcacgctgc gcgatctcac ggacgaatac gaagcggtgc gccgcgccac
     6121 gatcgagctc ttccgccact tcgatgagac ccagt
//
LOCUS       DASTKC010000203         9690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2056283, whole genome shotgun sequence.
ACCESSION   DASTKC010000203 DASTKC010000000
VERSION     DASTKC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056283"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..342)
                     /locus_tag="VFR25_08460"
     CDS             complement(49..342)
                     /locus_tag="VFR25_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941123.1"
                     /translation="MDPSPSIPRRRFVKLLAASAASLAMTSDPARAAAKTTERHRAAA
                     SAPPKSTALDKELRTEKKSVADMLKAVRSYKLPAGSAPAMVFRPMKARRGRTS"
     gene            439..1695
                     /locus_tag="VFR25_08465"
     CDS             439..1695
                     /locus_tag="VFR25_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9941124.1"
                     /translation="MGARHVVVIGGGAVGLASADELLARGHRVTIVERGGPDHDCCSL
                     GNAGYISPSHFLPLAAPGLLGQAIRWMGNPESPFYVRPRPDPALAGWMWRFWLASSPA
                     RAHAAGPLMRDLNLASRSLFESLAADTHDAFSLVRRGCLTLFQTQRGLDREAEHAERS
                     RALGMDAEVLDAKAVAALEPGVSLDVTGGVFYPLDAHVTPSLFHATLTRRVAEKGGVF
                     LWNAESRGFSRDSRRIRAVATTQGDIQGDEFLVAAGAYSSTLVAPLGIGLPLQPGKGY
                     SLTMPKPPEMPTRPLLLQEVRVAITPMNGSLRFGGTLELGAWGPEINPPRIRGIVRSA
                     LKHLPAFREDHFQNLEAWSGLRPCSPDGLPYVGRFRRYENLSVAAGHAMMGLSMSLIT
                     GRVVAEILSDERPEYDLTLLDPDRYG"
     gene            1739..2935
                     /locus_tag="VFR25_08470"
     CDS             1739..2935
                     /locus_tag="VFR25_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9941125.1"
                     /translation="MNFGRAWRSEWLLDPDVTYLNHGTVGAPPRRVLEAQQKIRDEIE
                     RRPSEYLLRDLMPSDTGRSLLPEPLLRTAARKVARFVHAREADLAFVENATTGANAVL
                     RSLDLRAGDEIVATQFGYGGVTKAAHYVARRAGATVREATMPHPVEHPDAIVRAIEET
                     LTPRTRLVIVDHVTSETALVLPVREIADACRARGVPVLVDGAHGPGAVPVDVGALGAD
                     WYAANLHKWALTPRPCGFLWARDNRQVDLHPAVVTWGLDQGFTTEFDWGSTRDPSAFL
                     AAPEGIAFLEQWGAGVVLSHNHALAWEAGQRLEKAWDTDARAREDMIGCMITVPLPAA
                     AGSTGDEAHALRDALLFEDKIEIQLHEFRGRLWVRLSAQIYNDLDDVNRLARAVRARV
                     GAGTRS"
     gene            complement(2937..5012)
                     /locus_tag="VFR25_08475"
     CDS             complement(2937..5012)
                     /locus_tag="VFR25_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941126.1"
                     /translation="MDAGRHRHSAPHLTLIRSHAAPARDDGAWEDSLARESDAGNRVP
                     SSFGSDTTSGRHTMHVVFALLVTAVFAVFQLATWPLLEASPFLLFCAGAVIAASVAGF
                     EGGVITTLSSALVAQFFFITPHRLVPNDPKEALGLAGFLATGFLISLLVARLDRDRAR
                     LARVLDSVSDGFVSVDRDGRCRYANEAAAALEGRSREELVGEPFDLVPGVPLDRTGLR
                     DHEPARVEGYVANLERWIEVTSYPAEDGTTLLYRDVTARHEFDEAIQRAHDREREART
                     IAESASRAKDEFLAMISHELRAPLTSILGWLRLLQEGSMDEKETPRALDTIERNATAL
                     SHLVGDVLDVSRLVSGKLELDRRLLDPEPALRTAADAVRPLAQRKQIEIVVETYGARP
                     VFADAARLHQILWNLLANAVKFTPQGGRVHAALRTDVSDVVFTISDNGIGIRPEFLPH
                     VFDRFSQHDSSYSRRFTGLGLGLAIARNLAEMHGGSVEAKSAGEGQGATFVVRIPRAL
                     REESFPRMEPVPPRNGLEPSPFESGPGPDRDPESTPERLDRLPSLQGLRVLVVDDEPD
                     ARALVVEILTRRGANVRACPSAVEGRALFAQWRPDVLVSDIGLPEESGYSLIRSIRAL
                     GKEQGGDVPAIALTGYARLTNRLEALSAGYDLHIPKPVEPSELVLMVASVAGRLTEGP
                     LGHPRPYRT"
     gene            5541..6467
                     /locus_tag="VFR25_08480"
     CDS             5541..6467
                     /locus_tag="VFR25_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9941127.1"
                     /translation="MARSFWKGSLSFGLVEIPVRLQPATVSHDLAFTLLDRRNFAPVG
                     NRRYNKNTGREIEWNDVVRGYEYEPDQYVVLSDEELEAVNVKSTGTIEILEFVPSDSI
                     DSLYFETPYYIEPMKKASKSYALLRTALERTGRTGIARVVLRTRQRTAALVVRDSALV
                     LNLLRYAHELRSTSDLELPEGKAVKASEAEVRMAERLIGEMYAEWDPDQFQDEYHDEV
                     LALVEKKVRAGKTHEILEPEAAPKRPSARSDVVDLIPLLKRSLEERATAERKGTQGGG
                     SRKRAAHDSVSRRPAARKLGRAKTPVRKKRTA"
     gene            6471..8081
                     /gene="ligD"
                     /locus_tag="VFR25_08485"
     CDS             6471..8081
                     /gene="ligD"
                     /locus_tag="VFR25_08485"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9941128.1"
                     /translation="MPLTTYRKKRDFRKTPEPKGGGRAARRRTRLAYVIQKHAASHLH
                     YDFRLELDGVLKSWAVPKGPSLNPADKRLAVEVEDHPVEYGGFEGTIPKGEYGGGSVL
                     LWDRGTWEPVGDAHEGLRQGRLSFDLFGEKLKGTWHLIRMKGRDEDRKPTWLLFKEKD
                     AESRARGDVLRDRPESVKTGRAIEEVGKNGKVWHSDRPSARERPAPPPKPTRKGPPKR
                     DPGRKAPLRDPPPEEPKHPPVREPPDEAREATVAGVAITHPKRVLFPEAGITKRDLAS
                     YIEAVAERMLPHVKGRPLMLVRCPEGRTGGCFFQKHPGFSVPRSVRQITVRESKGNAT
                     YLAVSNVAGLVGLIQVGVIEIHIWGAREDRQDSPDRLVFDLDPDPSVPWSRTVHAALD
                     LRERLRALGLESFPKTTGGKGLHVVAPFRRGPSWDDVARVGSAVAASMIQDDPDGFTA
                     TLSKAKRRGKILIDTLRNRRGATWVAPYSPRAREGAPVSMPLAWNEVTPALRPSRFTV
                     RTAASRLRKKDPWGALPGVRQSLPRVRL"
     gene            8224..8631
                     /locus_tag="VFR25_08490"
     CDS             8224..8631
                     /locus_tag="VFR25_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941129.1"
                     /translation="MNRSPSRSWKDITTVAALAAVVLTLAVVQIPLLAETIQTELLGP
                     DPDDEGAAVHGAVMPALEAPIAPTTTEAGLPVEASVWLAPIAGLGTSTGLGWVGSTGA
                     MNASADGRQGFHVRLGGGLMAPLTPSVGVDIRG"
     gene            8980..9129
                     /locus_tag="VFR25_08495"
     CDS             8980..9129
                     /locus_tag="VFR25_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET9941130.1"
                     /translation="MLWTITILLFLLWLVGMVSNYTLGGFIHLLLLLAIVTMLIRIIQ
                     GRRPV"
     gene            complement(9235..>9690)
                     /locus_tag="VFR25_08500"
     CDS             complement(9235..>9690)
                     /locus_tag="VFR25_08500"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HET9941131.1"
                     /translation="QVERLEEIFQDMGKPASGKTCKGMSGIIDEGSEILHEDGDPSVI
                     DAGIIAAAQRVEHYEIASYGTLKTFARTKGDTKAARILEEILNEEKEADHRLTQIAET
                     SVNPEARDIEEDEWDEDEEAERPGGGTRPGRGMSASDSERRGKANGPWT"
BASE COUNT         1507 a         3299 c         3337 g         1547 t
ORIGIN      
        1 tgtcctcgga cagctcggtg acggtgcggt agaggacctc ctcgccgatc atgaggtcct
       61 cccgcgccgt gccttcatcg gacggaagac catcgcgggg gcactccccg cggggagctt
      121 gtagctccgc acggccttca gcatgtcggc cacgctcttc ttctcggtcc ggagctcctt
      181 gtcgagggcg gtgctcttgg gcggtgccga ggcggccgcg cgatggcgct ccgtggtctt
      241 tgcggcggcg cgcgccggat cggaggtcat ggcgagcgac gccgcgctcg cggcgaggag
      301 cttcacgaag cggcgtctcg gaatcgaggg agacggatcc atggattcct cctggttggg
      361 acgctaggga agcggtgacg ctaccaccgt gggcgtccgc gcgccagacg cgcggcgagg
      421 ctggtatagt tcgcctccat gggcgcgcgc cacgtcgtcg tgatcggggg aggagcggtg
      481 ggcctcgcgt ccgcggacga gctcctcgcg cgcggccatc gggtcacgat cgtcgagcgc
      541 gggggtcccg atcacgactg ctgctcgctc gggaacgcgg ggtacatctc cccgagccac
      601 ttcctgccgc tcgcggcccc aggcctcctc ggccaggcga tccgctggat gggaaatccc
      661 gagagcccat tctacgtgcg tccgcgtccc gatccggcgc tcgcgggatg gatgtggcgc
      721 ttctggctgg cgtcctcccc ggcgcgcgcg cacgccgcgg ggcccctgat gcgcgatctc
      781 aacctcgcga gccggtctct cttcgaatcg ctcgccgcgg acacccacga cgcgttctcg
      841 ctcgtgcgcc gcggctgtct cactctcttc cagacgcagc ggggcctcga ccgcgaagcc
      901 gagcacgccg agcggtcacg ggcgctcggc atggacgccg aggtactgga cgcgaaggcc
      961 gtcgccgcgc tggagccggg agtctccctc gacgtgacgg ggggcgtgtt ctatccgctc
     1021 gacgcgcacg tcacgccgag cctcttccac gcgacgctca cccgacgtgt ggcggagaag
     1081 gggggcgtgt tcctgtggaa cgcggagagc cggggcttca gccgcgactc gaggcgaatt
     1141 cgcgcggtcg cgaccactca gggggacatc caaggggacg agttcctcgt cgcggcagga
     1201 gcgtactcgt cgacgctcgt cgctccgctc ggcatcggcc ttccgctcca gccgggaaag
     1261 gggtacagcc tcacgatgcc gaagcctccc gagatgccga cgcggccgct cctcctccag
     1321 gaagtgcggg tcgcgatcac gccgatgaac ggatcgctcc gcttcggggg gacgctggag
     1381 ctgggcgcct ggggaccgga gatcaatccc cctcgcatcc gcggcatcgt gcggtcggcg
     1441 ctcaagcacc tcccggcatt tcgcgaggat cacttccaga atctcgaggc gtggtccggg
     1501 cttcgcccgt gctcgcccga cgggcttccc tacgtcgggc ggtttcgccg ctacgagaac
     1561 ctatccgtgg cggccggaca cgccatgatg gggctctcga tgtctttgat cacgggccgc
     1621 gtggtggccg agatcctctc ggacgagcgg ccggagtacg atctcacgct tctcgatccg
     1681 gatcgctacg gctgatggcc gagcgcgtgc cggcgccatt caccctcgga ggctctcgat
     1741 gaacttcggc cgcgcgtggc gcagcgagtg gctcctcgat cccgacgtca cctacttgaa
     1801 ccacggcacg gtgggcgcgc cgccgcgccg cgtgctcgag gcccagcaga agatccggga
     1861 cgagatcgag cggcgcccct cggagtacct tctacgagac ctcatgccgt ccgacacggg
     1921 gcggtcgctc ctcccggagc ctctgctccg caccgcggct cggaaggtgg cgcgcttcgt
     1981 ccacgcgcgc gaagccgacc tcgcgttcgt ggagaacgcg accaccggcg ccaacgccgt
     2041 gctccgctcg ctcgatctcc gggccgggga cgagatcgtg gccacgcagt tcggatacgg
     2101 cggtgtcacc aaggcggccc actacgttgc gcgccgcgcg ggcgcgaccg tgcgcgaggc
     2161 gaccatgccg catccggtcg agcatccgga cgccatcgtt cgcgccatcg aggagacgct
     2221 cacgccccgg acgcggctcg tcatcgtgga tcacgtcacg tccgagaccg cgctcgtcct
     2281 cccggtccgt gagatcgcgg acgcgtgccg cgcgcgcggc gtgccggtgc tggtggacgg
     2341 ggcgcatggc ccgggcgccg tacccgtgga cgtgggcgcc ttgggggccg actggtacgc
     2401 ggcgaatctg cacaagtggg cgctcacgcc gcggccgtgc ggctttctct gggcgcggga
     2461 caaccggcag gtggatcttc acccggccgt cgtgacctgg gggctcgacc agggattcac
     2521 gaccgagttc gactggggga gcacgcgcga tccatccgcg ttcctggccg cgcccgaggg
     2581 gatcgccttc ctggagcagt ggggggccgg cgtcgtgctc tcacacaacc acgcgctcgc
     2641 gtgggaagcg gggcagcgcc tagagaaggc gtgggacacg gacgcccgcg cgcgcgagga
     2701 catgatcgga tgcatgatca ccgtcccgct cccggccgcg gccggctcga cgggggacga
     2761 ggcgcacgcg ctccgcgacg cgctcctctt cgaggacaag atcgagatcc aactccatga
     2821 gttccggggt cggctctggg tgcggctctc cgcgcagatc tacaacgacc tcgacgatgt
     2881 gaatcggctg gcgcgcgccg tgcgcgcgcg ggtgggggct ggaacgcgct cctgaatcag
     2941 gtgcggtacg ggcgtggatg cccgagcggt ccttcggtga gccggccggc cacgctcgcc
     3001 accatgagca ccagctccga cggctcgacc ggcttgggga tgtggaggtc gtacccggcg
     3061 gagagcgctt cgaggcggtt cgtgaggcgg gcatagccgg tgagcgcgat cgcgggcacg
     3121 tctccgccct gttctttgcc gagcgcgcgg atgctccgga tcaagctgta gcctgactcc
     3181 tcgggcaggc cgatgtccga tacgaggacg tcgggcctcc actgcgcgaa cagggcgcgc
     3241 ccttcgaccg ccgatggaca cgcgcgcaca ttcgcccccc gcctcgtgag gatttcgacg
     3301 accagcgcgc gcgcgtccgg ctcgtcgtcc acgacgagca cgcgcaggcc ctggagcgac
     3361 ggaagacgat ccagccgctc gggagtggat tcggggtccc gatccgggcc ggggcccgat
     3421 tcgaacggcg acggctccaa gccgttccgc gggggcaccg gctccatacg ggggaacgac
     3481 tcctcccgca acgcgcgagg aatgcggacg acgaacgtcg ctccctgtcc ctcgccggcg
     3541 ctcttggctt ccacgctccc tccatgcatt tcggccaggt tgcgggcgat ggcaagcccc
     3601 agtcccagtc cggtgaaccg gcgtgaatac gagctgtcgt gctggctgaa tcgatcgaaa
     3661 acgtggggca ggaattcggg gcgaatcccg atgccgttgt ccgagatcgt gaagaccacg
     3721 tcggacacgt cggtgcggag ggccgcatgc acgcgtcccc cttggggcgt gaacttcacg
     3781 gcgttcgcga ggagattcca caagatctgg tggagacgcg ccgcatccgc gaagacgggg
     3841 cgcgctccgt acgtctccac cacgatctcg atctgcttcc tctgggcgag cgggcgcacc
     3901 gcgtccgccg ccgtccgcag agcgggctct ggatcgagga gccgccggtc gagctccagc
     3961 ttgccggaga cgagccgcga cacgtcgagc acatccccga cgagatggga gagcgccgtc
     4021 gcattccgct cgatcgtgtc gagcgcgcgc ggggtctcct tctcgtccat gcttccttcc
     4081 tggagcagac ggagccagcc caggatcgac gtgagcgggg cgcggagctc gtgggagatc
     4141 atggcgagga actcgtcctt cgcgcggctc gccgactcgg cgatggtgcg cgcctcgcgc
     4201 tcgcggtcgt gcgcgcgctg gatggcttcg tcgaactcgt gccgcgccgt cacgtcccgg
     4261 tagagaaggg tcgttccgtc ctcggcgggg tacgaggtca cctcgatcca ccgctccaga
     4321 ttggccacgt acccttccac ccgcgctggc tcgtggtccc tcagaccggt ccggtcgagc
     4381 ggcacgccgg ggacgagatc aaacggctct ccgaccagct cctcccggct ccgtccctcg
     4441 agcgcggcag ctgcctcgtt cgcgtatcgg caacgtccgt cccggtccac cgacacgaat
     4501 ccgtcgctca cgctgtcgag gacccgggcc aagcgcgcgc gatcgcgatc gaggcgcgcg
     4561 acgaggaggc tgatcaggaa cccggtcgcg agaaagcccg cgaggccgag cgcctctttg
     4621 ggatcgttgg gaacgagtcg atgcggcgtg atgaagaaga actgggcgac gagcgcgctc
     4681 gacagggtcg tgatcacccc gccctcgaat cccgccacgc tggccgcgat gacggcgccc
     4741 gcgcagaaaa gcaggaatgg gctcgcctcg agaagcggcc acgtggccag ctggaacacg
     4801 gcgaacaccg ccgtcacgag cagcgcgaac accacatgca tcgtgtggcg gccggaggtc
     4861 gtgtcggacc cgaaggagct cgggacccgg ttccccgcgt cgctctcgcg agcgagcgag
     4921 tcctcccacg cgccgtcgtc cctcgccgga gcggcgtggg agcggatgag cgtgaggtgc
     4981 ggagccgaat ggcggtggcg ccctgcgtcc atcactcctc cgtcagtcca tcaagtgctg
     5041 ggcccggccg tgccgttcca gctctacgag ggtcgagaaa cccgccccgc aacgcggaca
     5101 gttgtagggt gcgcgtccct cgggccgtcg ctcgacttcc tcggccgaac ggaacattcc
     5161 ggacgagcgc ctatcttcgg gcattccggt gtgctgcttc atgtgtcgat cgagctgctc
     5221 cgccgtggag aactcggcct ggcaacgggg gcaacggtac tgttccatga gtcctcccgg
     5281 catcctgtcg tcccggggcg gggatgcccg gagttccaag ctgccaccgg ttgtaagaag
     5341 ctccttcaaa gccgggcagg gctcaaccaa cccccggcct tcacccgact ggcgccccat
     5401 cgtccaagat ccatgattcc cttgtggttc cggccgtctg gaccggcccc acctgagccg
     5461 gaatcgtgga cgggtgcggt ggcgcgtgct ctgcatggag aggcaatgca agcggcgaac
     5521 cattggatag gaggaccgcg atggcccgat cgttctggaa ggggagtttg agttttgggc
     5581 tggtcgagat tccggtccgg ctacagcccg ccaccgtgag ccacgacctt gcgttcacgc
     5641 tcctcgaccg gcgcaacttc gcaccggttg ggaaccgccg atacaacaag aacaccgggc
     5701 gtgaaatcga gtggaacgac gtggtgcgcg ggtacgagta cgagccggat cagtacgtgg
     5761 tgctgagcga tgaggagctc gaggcggtga acgtgaagtc gaccggaacg atcgagatcc
     5821 tcgagttcgt tccgtccgac tcgatcgact ccctctactt cgagacgccc tactacatcg
     5881 agcccatgaa gaaggcgagc aagagctacg cgctcctgcg cacggcgctc gagcgcacgg
     5941 gacgcaccgg aatcgcgcgg gtcgtcctgc ggacgcggca gcgcaccgcg gcgctcgtgg
     6001 tgcgcgactc ggccctcgtg ctcaacctgc tccggtacgc ccacgagctt cggtccacaa
     6061 gcgacctcga gctccccgag gggaaggccg tgaaagcatc ggaggccgag gtccggatgg
     6121 cggagcggct gatcggggag atgtatgccg aatgggatcc ggatcagttc caggacgagt
     6181 accacgacga ggtcctcgcg ctcgtcgaga agaaggtgcg cgccggcaag acgcacgaga
     6241 ttctcgagcc cgaggccgcg ccgaaacgtc ccagcgcccg gtccgacgtc gtggatctga
     6301 tcccgctcct caagcgaagc ctcgaggagc gcgcgacggc ggagcgcaag gggacgcagg
     6361 gcggaggctc gcggaagcgt gcggcgcacg attccgtgtc gcgcaggccg gccgcgcgaa
     6421 agctcgggcg tgcgaagact cctgtccgga agaagcggac cgcctgagcc atgccgctca
     6481 ccacctatcg gaagaagcgc gatttccgca agacccccga gccgaaggga ggcggccgcg
     6541 cggctcgccg ccgcactagg ctcgcgtacg tgatccagaa gcacgccgcg tcgcacctcc
     6601 actacgactt ccgcctcgag ctggacgggg tgctgaagag ctgggccgtg ccgaagggac
     6661 cgagcttgaa tcccgcggat aagcggctcg cagtcgaagt cgaggaccat ccggtggagt
     6721 acgggggctt cgagggaacg atcccgaagg gggagtacgg agggggatcg gtgctcctct
     6781 gggatcgcgg tacctgggag ccggtgggag atgcgcatga ggggctgcgc cagggcaggc
     6841 tctcgttcga cctcttcggg gagaagctga agggaacgtg gcacctcatt cgcatgaagg
     6901 ggcgcgacga ggatcggaag ccgacgtggc ttctcttcaa ggagaaggac gcggaatcac
     6961 gcgcccgcgg cgacgtgctc cgggaccgtc ccgagagcgt gaagacggga cgcgccatcg
     7021 aggaggtggg caagaacggc aaggtgtggc actcggaccg gccctcggcg cgcgagcggc
     7081 ctgcgccgcc gccaaagccg acgcgcaagg gaccgcccaa gagggatccc ggcaggaagg
     7141 ctccgcttcg cgatccgccg cccgaggagc ccaagcatcc tccggttcgc gagcctccgg
     7201 atgaagcgcg cgaggcgacg gtggccggcg tggccatcac gcatccgaag cgcgtcctct
     7261 ttcccgaggc gggaatcacc aaacgggatc tcgcctccta catcgaagcc gtggccgagc
     7321 ggatgctccc gcacgtgaag ggacgtccgc tcatgctcgt ccggtgcccg gaagggcgca
     7381 caggcggctg cttcttccag aagcacccgg gcttcagcgt tccccgctcg gttcggcaga
     7441 tcacggtgcg cgagtcgaag gggaatgcga cctatctcgc cgtgtcgaac gtggcggggc
     7501 tcgtcggcct gatccaagtc ggcgtgatcg agatccacat ctggggcgcg cgcgaagacc
     7561 gccaggattc gcccgatcga ctcgtcttcg atctcgatcc cgatccctcg gtcccgtggt
     7621 cgagaaccgt gcacgccgcg ctcgaccttc gcgagcggct ccgagccctc ggcctcgagt
     7681 cctttccgaa gacgaccgga ggcaaggggc ttcacgtcgt cgcgcccttt cggcgcggtc
     7741 cgtcctggga cgacgtggct cgcgtcggct cggctgtggc cgcgtccatg atccaggacg
     7801 atcccgacgg attcaccgcg accctctcga aggccaagcg ccgggggaag atcttgatcg
     7861 acacgctgcg caaccggcgc ggcgcgacgt gggtcgcgcc gtactcgccg cgggcacggg
     7921 aaggcgcccc agtgtccatg ccgctcgcct ggaacgaagt cacgccggca ttgaggccga
     7981 gccgcttcac cgtccgaacc gcggcgtccc ggctccgaaa gaaggatccc tggggcgctc
     8041 tcccgggagt gaggcagagc cttccccggg tccgcttgta gaaacgattg cctcatcgca
     8101 cctagaggta cattcccgcc cacgcgcgtc cgcccgtaac cgctgttccc gcctcacaag
     8161 ctctctggca cggtcattga tagttgcctc tgcgatccta attctccatc gcacgaggtc
     8221 cccatgaacc ggtcaccgag ccgcagctgg aaagacatca cgaccgtcgc cgcactcgcc
     8281 gccgtcgtgc tcacgctcgc cgtcgtccag atcccgctcc tggcggagac gatccagact
     8341 gagctcctgg gtccggatcc ggacgatgaa ggggcagccg tgcacggcgc tgtcatgccg
     8401 gcgctcgaag cgccgatcgc tcccaccacg accgaggccg gtctgccggt cgaagcttcc
     8461 gtgtggctcg cgccgattgc gggcctgggc acgagcaccg gcctcggctg ggtggggagc
     8521 acgggcgcga tgaatgcgtc cgccgatgga cggcagggat tccacgtgcg gctcggcggg
     8581 ggcctgatgg cgccactcac tccgagcgtg ggggtcgaca ttcgaggata ggagcgctct
     8641 tcggagcgtc gaggaaagtc cagacgccga gcgatcgacg tcaggatgaa cgggaggagc
     8701 aaccaagagc cctcccggca gcccccgtgc cgcgcgcgcg ggggctttgt tttgccccct
     8761 gggctccgca cggcgcgcaa tcgtctttgt atccgctaca actccgccgc ggaaaagcgc
     8821 gcctgctcgc gcccgtgagc ggcgcaactc catggtttgt gggcttttcg tgctggcacg
     8881 gaacgtgccc tgatgcatga caggtgccgc cgcgctccgc ccgcgtccgg cgggcgattc
     8941 aggcggacct tcgaacttac cggacgcaag gaggccgaaa tgctttggac gatcacgatc
     9001 ctcttgttcc tgctgtggct cgtcgggatg gtctccaact acacgttggg cggattcatc
     9061 cacctgctcc tcctgctcgc catcgtgacg atgctgatcc gcatcatcca ggggcgccgg
     9121 ccggtctgag cgggagagga ccgcgaatcg acgcgtggtc cgatcgacga aaagggaagg
     9181 ctctcggatt cgagccttcc cttttgcatg tgcggccgaa gtcttgcccg cgaatcaagt
     9241 ccagggaccg ttggccttgc ctcgacgctc cgaatcgctc gcggacatgc cgcggcccgg
     9301 ccgcgtgccg ccaccagggc gctcggcctc ttcgtcctcg tcccactcgt cttcctcgat
     9361 gtcccgggcc tcgggattca cggaggtctc ggcgatctgg gtcaggcggt ggtcggcttc
     9421 cttctcctcg ttcaggatct cctcgagaat gcgcgcggcc ttcgtgtctc ccttggtccg
     9481 ggcaaaggtc ttgagcgttc cgtacgaggc gatctcgtaa tgctccacac gctgtgcggc
     9541 cgcgatgatc cccgcgtcga tcaccgaggg atccccgtcc tcgtggagga tctccgatcc
     9601 ttcatcgatg attccggaca tgcccttgca ggtctttcca ctcgcgggct tgcccatgtc
     9661 ctggaagatc tcctcgaggc gctccacctg
//
LOCUS       DASTKC010000204        18229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1818863, whole genome shotgun sequence.
ACCESSION   DASTKC010000204 DASTKC010000000
VERSION     DASTKC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18229
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818863"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VFR25_08505"
     CDS             <1..1711
                     /locus_tag="VFR25_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9941132.1"
                     /translation="NGRDQDRELLSGITREQVDQAARALQSKLTDAEIERAVATMPEP
                     WRAIDGPRLTADLKGRRDRLPEAAKRFYELLSDKVDVTLTSEPERVDAVRQANGDLDL
                     TVRPVTGARAADPPGDPSPGEPSFHRVFRRGETSEVRLYCLAGDDRVTVTGPDGGIKL
                     RVVGGPGDDVLAARGSGPAKLSDSDGRNQADGASYDDHVYVPPPAPATAPWVPARDWG
                     SKTNRTPLLSYGPDLGFFVGLALDHRTYAFRKHPYATRHVLSAGWAFGSLSGKAEYKG
                     EFRRENRRDFWSFRGYASGIDVLRFYGLGNGTANLEGDRSRVRAIQFLAYPSYNALFG
                     QHSTFQIGPVFKQNKVRQDKAEDDSSFVFFTQPYGYGNFATLGAHTEAVFDSRDNPAF
                     PRKGFVLAARGTVYPKALDAEETFGEVNGNVNTYVSTGSRFTIALRGGGKKLWGQYPF
                     YESAKIGGGSVGEGTFGEPDYTLRPFAVARFQGDASLWGNAEARLRLFSTTILLPSHV
                     GLLAFADAGRVWYGGEDSDVWHGGGGGGGWLSFLGDRGALSLGYATSGEDNRLYFKGG
                     FTY"
     gene            1716..2177
                     /locus_tag="VFR25_08510"
     CDS             1716..2177
                     /locus_tag="VFR25_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9941133.1"
                     /translation="MRIGVISDTHGLLRPEALDALRGSEQIIHAGDVGDAAILDALRA
                     VAPLIAVRGNVDGDLAALLPKTAILRAGELAIYVHHGNDRRNLWPPPGTDVVISGHTH
                     EPRVEEVDGVLHLNPGSAGPRRFHLPVTVAILTVEDGRPSARIVPLLPQHP"
     gene            2243..4516
                     /locus_tag="VFR25_08515"
     CDS             2243..4516
                     /locus_tag="VFR25_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941134.1"
                     /translation="MELEPGKKFSHYKILEMLGEGGQAFVYRALDLHLKRDVALKVPK
                     GGRWGDMATRERFRQEALALSQVNHPNVAVIHEFGESHGLQFIAMEFIPGITLGHRIS
                     AGPVREMEALNLGVQIAEGLAAAHHAGVIHRDLKPHNIRLLNESWAKIVDFGLAKRLP
                     RPTDTGNPSLTETSKVVGTVPYMSPEQIRGEADARSDVYALGAVLYQTATGKRPFPQE
                     DLPLLIYAIAYEPPRPVRSLNPAVSPTFERMILRALEKEPDLRYPSAKDAVAALKEIR
                     DRAHSGGPAHDDPATPPLIRSLAVLPLEDLSLESEGDYFADGITEAIIVDLGKLKGLS
                     VRGRVSVMRFKRTTKPIPEIARELDVDALLEGTIQRVGDRVRTSVRLMRAVPEESIWA
                     ETYDRRLMDVLTLQSDIARSVAREIELKMKPRESAFVRTPAADAEALRLYMIGRQQWN
                     RRDLDGLKAAIHCFEGVVARSPDFALGFAGLADCFTILGNWSVLAPRDVYPKAKQAAL
                     RALALDPNLGEAHVALAFAEYLYDWKWKQAEEGFRRAIELNPSYAQGHSWYATFLSAM
                     GRHDEAIAEARRGQDLDPLSPIISAIAAWIHYEARRYEEAIERCRQDLKMNPIPQSYL
                     FMGLALAQLGRYDEAIAAFDRGVALAGGLTEMYAGLGFAYGASGRKTEARGVLEKLDR
                     LSKDRYVPPYSRAIVHTGMGERAEALELLEQACEERNTWLILLGVEPLFDSIREESRF
                     QRILEVIGLPSRSTAAV"
     gene            complement(4513..4974)
                     /locus_tag="VFR25_08520"
     CDS             complement(4513..4974)
                     /locus_tag="VFR25_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9941135.1"
                     /translation="MRGFTLIELSIALLVVAIVGAISITGYFSIMRSTHAMQAVGDLY
                     AIRAAAYMNYGDTAKWPEDLSPGIPPTELVSRLPAGFEFVRRDYRLDWDNWIGQHYAP
                     GDPRSAVLVGVSIVSNDPKLLDTIQSLMTRTYIIRTSPTKATLRIIGPGGL"
     gene            complement(5094..6776)
                     /locus_tag="VFR25_08525"
     CDS             complement(5094..6776)
                     /locus_tag="VFR25_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET9941136.1"
                     /translation="MIDPTRLNAWLPKSVLCLRGYTSRDFLHDLIAGLTVGLVALPLA
                     MAFAISSGVPPQAGIYCAIVAGFLISALGGSKTQIGGPTGAFVVVVSGIILHHGIDGL
                     FMCTLMAGVMLIVLGATGLGTAVDYIPRPVVVGFTNGIAVVIASTQIRDVLGLQIAHS
                     SGAFLPRLGELVAALPTISAPAAILGGTALATMILFATLWRKVPGAIVVLLGSTLAVA
                     LFALPVETIGSRFGGIPSGLPRVAVPTFRPELLGSLLPSAITIALLGAIESLMSAMVS
                     DRMTGDRHNPNVELIAQGIANVASPLVGGLPATGAIARTATNIRSGARSPIAGMVHAI
                     TLLLVLLLAAPLARFVPLPALAAILLVVAYNMGDWGEIPELLRLGKSDISVWLITFAL
                     TVFADLTAAVEVGMILAALLFIRKVSVTTSVEEITEAQVEEERRHRILDRDIPDDVTV
                     LRIRGPFLFGTTEKLADLAAEADPTPIVILRLRDMTAIDATGLTAIEDLADSLRARGS
                     TLLLCGAREQPALLMRKAGFDRHVGARNLCSDIEGALARAEEIRRQSGQAVQ"
     gene            complement(6873..9476)
                     /locus_tag="VFR25_08530"
     CDS             complement(6873..9476)
                     /locus_tag="VFR25_08530"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET9941137.1"
                     /translation="MRHKRVSGLLGLAVTLLFAPAAQGLEGFDPAVPGGADTDSSDGR
                     RAAAPTITAHRLNGSGDVSLDGKLDEPAWKDVPAGRGFRQWDPDRGAEPSEQTVFKVV
                     YDEDAVYFGIACLEKDPSKIVAKLSRRDRDAGCDIVSVYIDPYHDHTTGYNFKVSATG
                     VQQDAYIFNDGDTDVDWDAVWQAETSRDERGWYAEIRIPFSAIKYRTEPVMTWGLQVY
                     RYMQGRGENTGWVIWDRKEHGTVSRWGNLTGLASVHSPRQIELLPYFVQSATDPSIQG
                     SGDDVHGFRNVGADLRYGITPDLSLNATVQPDFGQVEADPAVLNLSPFETFYDEKRPF
                     FIEGSRFFQHPDFNLFYSRRIGTGDENTRIRYAAKLTGKSFGGVSVAALAASTDITQE
                     GQAHNLLKNGDQLTRYFVGRFGKEFTGGRNRFNLMQTAVLRTGSRDLYGDAGSREAYT
                     SGFDFDLNSKNRQFNMQGSMVGSIIDPETSTSDPTITGAQTYGTGGALDIRKRGGKLQ
                     GGISGRWESDKLQINDMGFLESPDEINTSVYLYKPYNPEGKSKTWNRGEFNLNANRSW
                     IYAARTGYDVNTGLPAWSYGAAHPQYSHVETSTWLQFRNYKEFWMGLSYNGEGTHRYE
                     TRGGPLIREPHTYGGWMGVSSDSRKNFVLTLEGNYFVDTAKNIGNHYTGTVKWNQSSS
                     INHSISLNFDRRIDDTQWLANENSADYPNARGIGGVSYIFGDINQQTFDITLRSNVLF
                     SRNQSLEIYAQPFITTGDYTRVRELIHPDSYDFLPFVDPVNVQSGQPGYTVNDWDFSY
                     AAVNLNLVYRWEYRPGSTFYLVWTQSRSTYEDRYNMGSAHFSPDIGGGQAFHNEPENR
                     VLAKVTYWIAI"
     gene            complement(9595..10743)
                     /locus_tag="VFR25_08535"
     CDS             complement(9595..10743)
                     /locus_tag="VFR25_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9941138.1"
                     /translation="MALTCELDPRGGTLNPNPSTKVDTDIHRASTLPSRFYSDPPMLD
                     RIRERVFARSWQFVADLERVRVPGAVHPVTLLEGFLDEPLLLTRDSKDQLHCLSNVCT
                     HRGNLVAQGDAVTQVLRCRYHGRRFTLDGRFQSMPEFDGAEGFPSAADNLPKVPYAEW
                     ERFIFASLDPATTLDKLIQPMRERCGFLPIRESAFDATRAREYVVRANWALYCDNYLE
                     GFHIPFVHAGLAGALDYGSYRTEVFPLSNLQVGVAADGEDAFELPKGHPDHGQRIGVY
                     YWWLFPNTMFNFYPWGISINVVRPLAVDRTKVHFLPFVWDPSKLDRGAGAALDRVERE
                     DESVVESVQKGVRSRLYDRGRYSPKREQGVHHFHQLLMESLAEDGTSS"
     gene            10860..11531
                     /locus_tag="VFR25_08540"
     CDS             10860..11531
                     /locus_tag="VFR25_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941139.1"
                     /translation="MTLSYNATRLVLTAVLLLILFVDRTADAQYLYLDTTGDGLNFYH
                     EISIGHLDAPADCLFYPGERDVDVYLVTNTNADGTVPTCSTGQSYSVSSYELLFSGLA
                     GVVPTGWTDAMGFDTSTLDGDTDGFVASGMNAWVGRMGAGRVPGTYKLGTLHVVVTGK
                     SFLQIVAQSSSFPTGTTSFASACPGNQLDNTIRLGDDFTAAFPTCYPDPVLPTTWGKV
                     KQHYK"
     gene            11731..14007
                     /locus_tag="VFR25_08545"
     CDS             11731..14007
                     /locus_tag="VFR25_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9941140.1"
                     /translation="MLLLYIVIAVAIVFAVPPIRRALVTRWIMPILAGALPKMGDTER
                     IALEAGTVWWDAELFSGRPRWKKLLEFKPKPLSPKERAFLDGPVEELCRMVHDWDVLQ
                     KGDLSPEAWAHLKKHRFFGMIIPESYGGLGFSALAHSAVITKLSSRSVTAAVTAMVPN
                     SLGPAELLLHYGTEAQKNYYLPRLATAEEIPCFALTEPNAGSDATAQRSEGVVCKGTF
                     EGREVLGMRLNWSKRYITLAPAATVLGLAFRLRDPDKLLGGQEDLGITCALLPVRLPG
                     VKIGERHDPLGVPFLNGPTFGTDVFAPLDFIIGGPANAGKGWRMLMDCLAAGRGISLP
                     SLSVGAAELATRTTSAYAGVREQFGLPIGRFEGIEEPLARIAGLTYMLDAARKLTAGA
                     IDAGEKPAVISAIVKRYATEGMRAVVNDAMDIQAGAAISRGPRNILSSPYASVPVGIT
                     VEGANILTRSLIVYGQGAIRCHPYVQSEMRAVAEKDLVAFDRAFFGHVGHTIGTGFRA
                     FGMAIFGGGTGGSGVSGAAGPMFGKLSRYAAAFALISDAAMATLGGTLKRRERLSGRL
                     ADALAWMYLASAMLKRFVDDGQPKRDLPYARWAAAHAFHQIEVALTGVLENLPSRPTA
                     WMLRLAAFPLGAHVRPVPDREVAAVAHSVLNDAEAREHLTADIFVPPPGEPGLGALEA
                     AYRLSLAAAPAEKKLKDAVRARKLKPLDGCALFDAGVEAGLISDAERRQLCEALKARE
                     DVIQVDAFGAKEYAEQRR"
     gene            complement(14037..16583)
                     /locus_tag="VFR25_08550"
     CDS             complement(14037..16583)
                     /locus_tag="VFR25_08550"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9941141.1"
                     /translation="MAARPGSRRPLLYACFFASGAAGLILEIVWSKYLSLLLGNSIYG
                     VSTVVAAFLGGLGIGAAIGGRWAARTREPLLLYARLELVVAVLGMMSPLGFALAKPLF
                     AWLNGLFLGHGAVFLLLRFLVLFAALLIPTTAMGATLPLLVSDFSRRDREGSGAAVAR
                     LYAVNTAGAVLGVVAAGFLAIPALGLWKTAAVAAAIDLIVFATILFGRPAAPPGVAAD
                     GAGAAAVVRDDGAAAPPWHRWILPLFALSGFTAILYEVSWTRILAVPFGGMIYAFSAI
                     LAIYLAGIALGAATASRVLKLWRAPVTLFGILQALLAAAVGIAAHQFEHLPHWQTTVM
                     VHANGSTSSLLWGEAGITAWIIFPACFLLGALFPTAVAIRRTARSEAGASVGAVYAFN
                     TIGSIAGSILTAFILIPWLGTLHSILAAAALNAALGVLALVTGEGRALPRRAAALLVL
                     IAAGAYAWVGMPSWNAERMSLGLVRLLRSYWLYGKEDVTHRMIDQVGASKELERLLFY
                     KEGRVAAVTVVETGKRRALLINGKTDATTGVGEDMAQQVMVGQLPVLLANQAQSVCIV
                     GYGSGVTTGAVLTHPVRDVLTIELEGAVAEAAPYFDADAGTPLADPRHRLLIEDAGTY
                     LRSTRNKYDVIISEPSNLWIAGMADLFTRDFYETAASKLRPGGIFCQWVQCYQTSPAT
                     LRTIFRTLATRFPKGQLFFVDKSADLVILASPDREIGLDLDQLGAWFQNEKVARDLAR
                     VGVSSLPDLMRYYRGRLERVARETDGGPVNTDDNGWLEHKAPFDLLAGATSEGEMAWS
                     PDVAADLAASITSDRAKAAGILADAAARAKAVGNEGAVLGLAMAREQLLR"
     gene            16787..>18229
                     /locus_tag="VFR25_08555"
     CDS             16787..>18229
                     /locus_tag="VFR25_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941142.1"
                     /translation="MSAGIPLRLAVTGFLTLLWIAFEAARAFSAPSATNPVDQWPRRV
                     YRTEVPAPLKGDPSAPMSYRRVLLTPPPLEEVYVFNDSLDGHPLDDEGGWSHFDNSAG
                     PTAWHIDTFMACNNHAWWCGMVDSSWINDTNRAGYDNSWTQYLQHDAPTATVPAGSII
                     TLTAKYKINVEQGYDYGEVEYNDLDDLWLPLLHLTGKIPASGVGCDSFTVELPESSWV
                     KWQNHPTGAKPMPFRFVFHSDIERSSADGLYNGDGWIIDEVRVTKDGNQILFYDNMEN
                     GMGTWTRTTLPAVGDYYALENNVVTEDQCTDNRTNIWVDWDQVTRSLVPRIDDRLLTP
                     PVDIDRSGEVLIAFDVYRNLPLNACFYYSVNYRTRMIGGNWTAWMDPTDLLYYGSSKD
                     WIRQKIVLPLAAGKDSVQCMFIVKDYGQIYCGGSTGYSNLYPLFDNIAIGVRATTPPI
                     FIPRDLDLFQDTFKTTAFFGNDNFNTPLGDS"
BASE COUNT         3117 a         6040 c         6095 g         2977 t
ORIGIN      
        1 gaacggacgc gaccaggacc gcgagctcct gagcgggatc acgcgcgagc aggtggacca
       61 ggccgcgcgc gcgctccagt ccaagctcac ggacgcggag atcgagcgcg cggtcgcgac
      121 catgcccgag ccgtggcgcg cgatcgacgg accgaggctc accgccgatc tcaagggtcg
      181 ccgggaccgg cttccggagg cggcgaagcg gttctacgag ctcctctcgg acaaggtgga
      241 cgtgacgctc acgagcgagc cggagcgcgt ggacgcggtg cgccaggcga atggcgacct
      301 cgatctcact gtgcgccccg tgacgggggc gcgggccgcg gaccctcccg gggatccgtc
      361 gccgggcgag ccctcgttcc atcgcgtctt ccgtcgcggc gagacgagcg aggtcaggct
      421 ctattgcctt gccggcgacg atcgagtgac cgtgaccggg ccggacggcg ggatcaagct
      481 ccgcgtcgtc ggtggtcccg gcgacgacgt cctcgccgcg cgcggctcgg gcccggcgaa
      541 actctccgac tcggatggcc ggaaccaggc ggacggcgct tcgtatgacg accacgtcta
      601 cgtcccgccc cccgcgcccg cgacggcccc ttgggtgccc gcacgcgact ggggctcgaa
      661 gaccaaccgc acgccgctgc tctcgtacgg tcccgatctg ggcttcttcg tgggcctggc
      721 gctcgaccat cgcacctacg cgttccggaa gcacccgtac gccacccgcc acgtgctcag
      781 cgccggttgg gcgttcggga gcttgagcgg caaggcggag tacaagggcg aattccgccg
      841 cgagaaccgg cgcgacttct ggtccttccg cgggtacgca tcgggcatcg acgtgcttcg
      901 cttctacggg ctcggcaacg gcacggcgaa cctggaaggc gatcggagcc gcgtgcgcgc
      961 gatccagttc ctcgcctatc ccagctacaa cgcgctgttc gggcagcact ccacgttcca
     1021 gatcggtccc gtcttcaagc agaacaaggt gcgccaggac aaggcggagg acgattcatc
     1081 gttcgtcttc ttcacgcaac cctacggcta cggcaacttc gcgacgctcg gcgcgcacac
     1141 cgaggcggtg ttcgacagcc gcgacaatcc ggcgtttccg aggaagggat tcgtgctcgc
     1201 cgcgcgcggc acggtctacc ccaaggcgct cgacgccgag gagacgttcg gcgaggtgaa
     1261 cgggaacgtg aacacgtacg tctcgacggg cagccgcttc acgatcgcgc tccgcggcgg
     1321 cggaaagaag ctctggggcc agtatccctt ctacgagtcg gcgaagatcg gcggcgggtc
     1381 ggtgggcgag ggcaccttcg gcgagcccga ctacacgctc cgtcctttcg cggtcgcacg
     1441 cttccagggg gatgcgtcgc tctggggcaa tgccgaagcg cggctccggc tcttcagcac
     1501 cacgatcctc ctgccgtcgc atgtcggcct cctcgccttt gccgacgccg gccgcgtctg
     1561 gtacggcggc gaggactcgg acgtgtggca cggcggcggc ggcggtggcg gctggctctc
     1621 gttccttggc gatcggggcg cgctatccct cggctacgcc acgtccggtg aggacaaccg
     1681 cttgtacttc aagggcggct tcacgtacta gcccgatgcg gatcggcgtc atctcggaca
     1741 cgcacggact cctccgcccc gaggcgctcg acgcgctccg cggctccgag cagatcatcc
     1801 atgccgggga cgtcggcgac gcggcgatcc tggacgcgct ccgcgccgtg gcgccgctca
     1861 tcgcggtgcg cgggaacgtc gacggagacc tcgccgcgct ccttccaaag acggcgatcc
     1921 tccgtgcggg agagcttgcg atctacgtcc accacgggaa cgatcggcgg aacttgtggc
     1981 caccccccgg aacggacgtc gtgatcagcg ggcacacgca cgagccgcgg gtggaggaag
     2041 tggatggtgt gctccacttg aaccccggga gcgccggccc gaggcggttc cacctgccgg
     2101 tgacggtcgc gattctgacc gtcgaggacg ggcgcccgag cgcccggatc gtgccgctcc
     2161 tgcctcagca cccgtagacc tacggagctg gacgcggtac cctggtgcgc cttcccgcaa
     2221 cacccacccg ggggacgatc gcttggagct cgaaccgggc aagaaattct cacactacaa
     2281 gatcctcgaa atgctcgggg agggaggcca ggccttcgtc tatcgagcat tggacctgca
     2341 cttgaagcgc gatgtggccc tgaaggtgcc gaagggcggc cggtggggtg acatggccac
     2401 gcgcgagcgg tttcggcagg aggcgctcgc gctatcgcag gtgaaccatc cgaacgtggc
     2461 ggtgatccac gagttcggtg aaagccacgg ccttcagttc atcgcgatgg aattcatccc
     2521 ggggatcacg ctcggccacc ggatcagcgc cggtccggtc cgcgagatgg aggctctcaa
     2581 cctgggcgtc cagatcgccg agggactcgc cgcggcgcat catgccggcg tcatccatcg
     2641 agacttgaag ccgcacaaca tccggctcct gaacgagagc tgggccaaga tcgtggactt
     2701 cggcctcgcg aagcggctgc cacgccccac ggacaccggc aatccctcgc tcacggaaac
     2761 gagcaaggtg gtgggcacgg tcccgtacat gtcccccgaa cagatccggg gcgaggcgga
     2821 cgcgcgaagc gacgtgtacg cgctcggcgc cgtgctctac cagacggcga ccgggaagcg
     2881 cccattcccg caggaagatc tcccgctgct catctacgcg atcgcctacg agccgccgcg
     2941 tccggttcgt tcgctcaacc cggccgtctc gcccacgttc gaacggatga tcctgcgcgc
     3001 gctggagaag gagcccgacc tccggtatcc cagcgcgaag gatgccgtgg cggcgttgaa
     3061 ggagattcgg gatcgtgccc actccggcgg gccggctcac gacgatcccg cgacccctcc
     3121 gctcatccgc tcgctggccg tgcttccgct cgaggatctg tcgctggaat cggaaggcga
     3181 ctatttcgcg gatgggatca cggaagccat catcgtcgat ctgggaaagc tcaagggcct
     3241 gagcgttcgt ggccgggtgt cggtcatgcg cttcaagcgc acgaccaagc cgatccctga
     3301 gatcgcgcgc gagctggatg tggacgccct cctcgaaggg accatccagc gagtggggga
     3361 ccgggtccgc acgagcgttc ggctgatgcg agccgtcccc gaagagtcga tctgggccga
     3421 gacgtacgac cgccggctga tggatgtcct cacgctccag agcgacatcg cccgctccgt
     3481 cgcgcgcgag atcgagctca agatgaagcc aagggagtcg gccttcgtgc ggacgcctgc
     3541 cgccgacgcc gaggcgctac ggctctacat gatcggccga cagcagtgga accgtcgcga
     3601 tctcgacggg ctcaaggccg cgatccactg cttcgagggg gtcgtggcca ggtcacccga
     3661 cttcgcgctc ggattcgcgg gcctcgcaga ctgcttcacg attctcggca actggagcgt
     3721 gctcgcgccg cgcgatgtct acccgaaggc gaagcaggcc gcgctccggg cgctcgcgct
     3781 cgacccgaat ctgggcgagg cgcacgtcgc cctggcgttc gcggagtacc tgtacgactg
     3841 gaaatggaaa caggcggagg agggatttcg acgcgccatc gagctcaacc caagctacgc
     3901 gcaaggtcac tcgtggtacg ccacgttcct gtcggccatg gggcggcacg atgaggcgat
     3961 cgcggaagcc cggcggggac aggatctcga cccgctgagt ccgatcattt ccgcgatcgc
     4021 ggcgtggatc cattacgagg cgaggcgtta cgaagaggcc atcgagcgct gccggcaaga
     4081 tctcaagatg aatccgattc cacagtccta tctcttcatg ggactcgcgc tcgcgcagct
     4141 gggccggtac gacgaggcca ttgcggcgtt cgaccgcggc gtggcgctcg ccggcgggct
     4201 gaccgagatg tacgcggggc tcggcttcgc gtacggagcc tcgggcagga aaacggaggc
     4261 acgcggcgtg ctcgagaagc tggaccgtct ctccaaggat cgttacgttc cgccctactc
     4321 ccgcgcgatc gtgcacaccg gcatgggcga gcgtgcagag gcgctggagc tcctcgagca
     4381 ggcgtgcgag gagaggaaca cgtggctcat tcttctcggc gtcgaaccgc tcttcgattc
     4441 gatccgcgag gagtcccgct tccagagaat cctggaggtg atcggactcc cgagccgctc
     4501 gaccgccgcc gtctagaggc cgccggggcc gatgatccgg agtgtcgcct tggtcggact
     4561 cgtgcgaatg atgtaggtgc gggtcatgag cgactggatc gtgtcgagga gcttcgggtc
     4621 gttcgagacg atcgaaacgc cgaccaggac cgcggagcgc ggatcgccgg gcgcgtagtg
     4681 ctggccgatc cagttgtccc agtcgagccg gtagtcgcgt cgcacgaact cgaaccccgc
     4741 cggcagccgg ctcacgagct ccgtgggggg aattccgggg gacaggtcct cgggccactt
     4801 cgccgtgtcc ccgtagttca tgtatgccgc cgcacggatg gcgtagagat cgcccaccgc
     4861 ctgcatggcg tgcgtgctcc gcatgatgct gaagtacccc gtgatcgaga tggcgcccac
     4921 gatcgccacc acgaggagcg cgatcgacag ctcgatcagg gtgaaccctc tcattccccg
     4981 cttcatcgtc cctcgaccgt gggttttcga ccgcgcgcgg cgatgctcct ccgcgctcga
     5041 tctatcggca cgtggcctgc ggatgttgat ggagtggggg ggacggccgg tggctactga
     5101 accgcctggc ccgactggcg gcggatctcc tcggctcgcg ccaaagcgcc ttcgatgtcg
     5161 gagcacaggt tcctcgctcc gacatggcgg tcgaacccgg ccttccgcat gaggagcgcc
     5221 ggctgttctc gcgcgccgca gaggaggagc gtcgagccgc gagcacgcag ggagtccgcg
     5281 aggtcctcga tggcagtgag acctgtcgcg tcgatggcgg tcatatcgcg aagtcggagg
     5341 atcacgatcg gcgtgggatc ggcctcggcc gccaggtcgg ccagcttctc cgtcgttccg
     5401 aagaggaaag gtccccggat gcgaagcacc gtcacgtcat ccgggatgtc tcgatccagg
     5461 atccggtgcc gccgctcctc ctcgacctgt gcctcggtga tctcctccac gctggtcgtc
     5521 acggacacct ttcggatgaa gaggagcgcc gcgaggatca ttcccacttc caccgcggcg
     5581 gtgaggtctg cgaagaccgt gagggcgaac gtgatgagcc agacgctgat gtccgacttg
     5641 ccgagcctca agagctccgg gatctcgccc cagtcgccca tgttataggc gaccacgaga
     5701 agaatggccg cgagcgcggg caacgggacg aagcgggcca acggagccgc gaggaggagc
     5761 acgagcagca aggtgatcgc gtgaaccatg ccggcgatcg gggagcgcgc gccggaacga
     5821 atgttggtcg ctgtacgggc gatcgcaccg gtggcgggaa gtccgcccac gagcggcgat
     5881 gccacgttgg cgattccctg ggcgatcagc tcgacgttgg ggttgtggcg atctcccgtc
     5941 atccggtccg agaccatggc ggacatgagc gactcgatgg ccccaaggag cgcgatagtg
     6001 atggctgatg gcaagaggga gccgaggagc tcggggcgga acgtgggcac ggcgacgcga
     6061 gggagtccgc tcgggatgcc gccgaatcgt gagccgatcg tctcgactgg aagcgcgaag
     6121 agcgcgacgg ccagagtgga accgaggagc accacgatcg cgcccggcac cttcctccat
     6181 agagtggcga acaggatcat cgtggcgagg gcggtcccac cgaggatcgc ggccggagcc
     6241 gatatcgtcg ggagggcagc cacgagctcc ccaagccggg gaaggaacgc ccctgaggaa
     6301 tgcgcaatct gcagaccgag gacgtcgcgg atctgcgtgc tcgcgatgac cacggcgatg
     6361 ccgttcgtga aaccgaccac caccgggcgt ggaatgtagt cgacagccgt gcccaatccg
     6421 gtcgcgccga ggacgatcag catgaccccg gccatgagcg tgcacatgaa caatccgtcg
     6481 atgccgtggt gcaggatgat cccggacacg acgacgacga aggctccggt ggggccgccg
     6541 atctgggtct tcgatccgcc gagcgcggag atcaggaatc ccgccacgat ggcgcagtag
     6601 atgcccgctt gaggcggcac gcccgaggag atggcgaacg ccatggccag gggcagcgcc
     6661 acgagaccca cggtgaggcc ggcgatgagg tcgtggagga agtcgcggct cgtgtagccg
     6721 cgaaggcaga ggacggactt cggaagccag gcgttgagcc gggtcgggtc gatcagcgca
     6781 ccgctcccgg tcgaaccgcg ggctgattgt gtagctctat gccccgaagg gtttggatcg
     6841 acagacgccg catgagcgcg gcgaagtata tgtcagatcg cgatccagta ggtgaccttg
     6901 gcgaggaccc ggttctcagg ctcgttgtgg aaggcctggc cgccgccgat gtcggggctg
     6961 aagtgggcgc tgcccatgtt gtaccggtct tcgtaggtcg agcggctctg ggtccagacg
     7021 aggtagaacg tgctccccgg ccggtactcc cagcgataga ccaggttcaa gttgaccgcc
     7081 gcgtagctga agtcccagtc gttcacggtg tagccaggct gaccggactg cacgttcaca
     7141 ggatccacga acggcaggaa gtcgtagctg tccgggtgga tcagctcgcg aacccgcgtg
     7201 tagtcgcccg tcgtgatgaa gggctgcgcg tagatctcca gcgactggtt ccggctgaag
     7261 aggacgttcg accggagggt gatgtcgaac gtctgctgat tgatgtcgcc gaagatgtag
     7321 ctcacaccgc cgatcccacg cgcgttcgga tagtcggccg aattctcgtt cgcgagccac
     7381 tgggtgtcgt cgatacggcg gtcgaagttc agcgagatcg agtggttgat cgagctcgac
     7441 tggttccact tcaccgtgcc ggtgtagtgg ttcccgatgt tcttggccgt gtctacgaag
     7501 tagttcccct cgagggtgag gacgaaattc ttcctcgagt cgctcgagac gcccatccag
     7561 ccgccgtacg tgtggggctc ccggatcagc gggccgcctc gtgtttcgta ccggtgcgtt
     7621 ccctccccgt tgtacgagag ccccatccag aattccttgt agttgcggaa ctggagccac
     7681 gtgctcgtct cgacgtggga gtactgcggg tgcgcggcgc cgtacgacca cgccggcagc
     7741 cccgtgttga cgtcgtatcc ggttcgcgcc gcgtagatcc agctccgatt cgcgttcagg
     7801 ttgaactcgc cccgattcca ggtcttgctc ttcccctcgg ggttgtacgg cttgtagagg
     7861 tagacgctcg tattgatctc gtcgggggac tcgaggaagc ccatgtcgtt gatctggagc
     7921 ttgtccgact cccatcgtcc gctgatcccg ccctggagct tcccaccgcg cttccgaatg
     7981 tcgagcgcgc ctccggtgcc gtatgtctgg gcgccggtga tcgtgggatc ggacgtggac
     8041 gtctccggat cgatgatcga gcccaccatc gacccctgca tgttgaactg ccggttcttc
     8101 gagttgagat cgaagtcgaa tccgctcgtg taggcctcgc gcgatccggc gtcgccgtag
     8161 aggtcacggc tcccggtgcg gagcaccgcg gtctgcatca ggttgaaccg gttccgtccg
     8221 cccgtgaact ccttgccgaa gcgccccacg aagtagcgtg tgagctggtc cccgttcttc
     8281 aacaggttgt gcgcctgccc ctcctgcgtg atgtccgtgg aggcggcgag ggcggcgacc
     8341 gagacgccgc cgaagctctt gcccgtcagc ttggccgcgt agcggatgcg cgtgttctcg
     8401 tcgcccgtcc caatgcgtcg cgagtagaac aggttgaagt cggggtgctg gaagaagcgg
     8461 ctcccctcga tgaagaaggg ccgcttctcg tcgtagaacg tctcgaacgg cgagagattg
     8521 agcacggccg gatcggcttc gacctggccg aagtcgggct gcacggtcgc gttgagcgag
     8581 aggtcggggg tgatgccgta ccggaggtcc gcccccacgt tgcggaaccc atgcacgtca
     8641 tctcccgacc cctggatgct cggatccgtg gcgctctgga cgaagtaggg aaggagctcg
     8701 atctggcgcg gcgaatggac gctcgccagc ccggtcagat tgccccaccg gctcaccgtg
     8761 ccgtgctctt tccgatccca gatcacccat ccggtgttct cgccgcgccc ctgcatgtac
     8821 cgatagacct ggagtcccca cgtcatcacg ggctcggtgc ggtacttgat cgcggagaag
     8881 ggaatgcgga tctcggcgta ccagccgcgc tcgtcgcgcg aggtctccgc ctgccagacc
     8941 gcgtcccagt ccacgtcggt gtcaccgtcg ttgaagatgt aggcgtcctg ctgcacgccg
     9001 gtcgcgctga ccttgaagtt gtagcccgtc gtatggtcgt gatacgggtc gatgtacacg
     9061 ctcacgatgt cgcagcccgc gtcgcgatcg cggcgtgaga gcttggcgac gatcttcgac
     9121 ggatccttct cgaggcacgc gatcccgaag tagaccgcgt cctcgtcata gaccaccttg
     9181 aagacagtct gctccgacgg ctcggcgccg cggtccgggt cccactggcg gaagccgcgt
     9241 cccgcgggga cgtccttcca cgcgggctcg tcgagcttgc cgtcgaggct gacgtcgccg
     9301 ctgccgttca ggcggtgcgc cgtgatcgtc ggggccgctg cccgacgccc gtccgagctg
     9361 tccgtgtccg ccccgcccgg gaccgcgggg tcgaaaccct cgagcccctg agcggcgggg
     9421 gcgaagagga gcgtcaccgc gaggccgagg aggccggaaa ctcgtttgtg ccgcataggg
     9481 atcgactcct taacgggctc gaatgcggga acgcgccggg gggaccgctc acctcaagta
     9541 cgtggaccgg aatgtattcg tttcgacaga gggagggggg aaagcgttga aagactagct
     9601 cgacgttccg tcctcggcca gcgattccat gaggagctgg tggaagtggt ggaccccctg
     9661 ctcccgcttg ggggagtagc ggccccggtc atagagcctg gaccggacgc ccttctggac
     9721 gctctccacc acggattcgt cctcgcgctc cacgcggtcg agcgcggccc ccgctccacg
     9781 gtcgagcttg gacggatccc acacgaaggg caggaagtgg accttggtgc ggtccaccgc
     9841 gagtgggcgg accacgttga tcgagatccc ccacgggtag aagttgaaca tcgtgttcgg
     9901 gaagagccac cagtagtaga cgccgatccg ctggccgtga tccgggtggc ccttgggaag
     9961 ctcgaacgcg tcctcgccgt ccgccgccac gccgacttgc aggttcgaga gcgggaagac
    10021 ctccgtgcga tagctgccgt agtcgagggc tcccgcgagt cccgcgtgca cgaagggaat
    10081 gtggaagcct tcgaggtagt tgtcgcagta gagcgcccag ttcgcgcgaa cgacgtactc
    10141 gcgcgcgcgc gtggcgtcga aggccgattc gcggatcggg agaaagccgc agcgctcgcg
    10201 catcggttgg atgagcttat cgagcgttgt cgccggatcg agggaagcga atatgaatcg
    10261 ctcccattcc gcatacggca ctttgggaag gttgtccgcc gcagagggaa atccctcggc
    10321 gccgtcgaac tccggcatcg actggaaccg cccgtcgagc gtgaagcgtc gcccgtggta
    10381 ccggcagcga aggacctgcg tgaccgcatc gccttgcgcg accaggttgc cccggtgcgt
    10441 gcagacgttg gaaaggcaat ggagctggtc cttggaatcc cgggtcagga ggagcggctc
    10501 gtcgagaaat ccctcgagga gcgtgaccgg gtgcaccgcc cccggcactc gcacccgctc
    10561 caggtctgcc acgaactgcc acgagcgcgc gaaaacccgc tcccggatcc ggtcgagcat
    10621 gggagggtcc gagtagaagc gcgagggcaa ggtcgaggcc ctgtggatgt cggtatcgac
    10681 cttggtcgat ggattcgggt tcagggttcc tccgcgcggg tcgagctcac aagtgagggc
    10741 catactataa gttagttccg tgcggtctgc atcagatgtg aaccgtgacc gggccagggc
    10801 ggaacgggag taagatcatg ggtaaggggc cggtccagag ctcgattgga gggccaagca
    10861 tgaccctgag ctacaacgca acgcgtcttg tcctaacagc cgttctcctg ctgatcctgt
    10921 ttgtagaccg cacagccgac gcccagtacc tctacctcga tacgactggc gacggactga
    10981 acttctacca cgagatctcg atcggccatt tggatgctcc ggccgattgt ctcttctatc
    11041 ccggcgagag agacgtggac gtgtatctcg tcaccaacac gaacgccgac ggcacagtcc
    11101 caacgtgcag caccggacaa tcctattcgg tcagctccta cgagcttctg ttcagcggcc
    11161 tggcaggggt cgttccaacc ggatggacgg acgccatggg gttcgacacc tcgacactcg
    11221 acggtgacac ggatggattc gtcgcgtccg gcatgaacgc atgggtgggg cggatgggag
    11281 ccggccgggt cccaggcacg tacaagctcg gaacgctcca cgtcgtagtg acggggaaat
    11341 cgttcctgca gatcgtggct caaagctcca gtttcccgac cggcactacg agcttcgcgt
    11401 cagcatgtcc tgggaatcaa ctcgacaaca cgatccgctt aggcgacgac ttcaccgcag
    11461 cgtttccgac gtgctaccca gaccccgttc ttccgacgac ctggggcaag gtcaagcagc
    11521 actacaaatg actttccgac gcatcgcaaa ggggggcgcg acacgccgcg cccccctttc
    11581 aaggtcccgc tctcctgtaa ccccttcata agtaggcccc tctaggagac ctcaggctac
    11641 gttcatggtc cggtcccctc tgccgatacc ttgtatactc gatagcagtt agcgccgttt
    11701 tccagggacc ggaaccgagg tcaatcgagc atgctcctcc tctatatcgt gatcgctgtc
    11761 gcgatagtct tcgccgtgcc gccgatccgc cgcgccctcg tcacccgctg gatcatgccg
    11821 atcctcgccg gggccctccc gaagatgggc gataccgagc gcatcgcgct cgaggcgggg
    11881 acggtctggt gggacgccga gctcttctcg ggccggccgc gctggaagaa gctcctcgag
    11941 ttcaaaccga agcccctcag tcccaaggag cgggccttcc tcgacggtcc ggtcgaggag
    12001 ctgtgccgca tggtccacga ctgggacgtc ctccagaagg gggacctctc gcccgaggcc
    12061 tgggcgcacc tcaagaagca ccgcttcttc ggcatgatca tccccgagtc gtatggcggc
    12121 ctcggcttct ccgcgctcgc gcactcggcg gtgatcacga agctctccag ccgcagcgtc
    12181 accgccgctg tcacggcgat ggtgccgaac tcgctcgggc cggccgagct cctgctccat
    12241 tacgggacgg aagcgcagaa gaactactac ctgccgcggc tcgcgacggc cgaggagatc
    12301 ccgtgtttcg cgctcacgga gccgaatgcg gggagcgacg cgacggcgca gcggagcgag
    12361 ggggtcgtct gcaagggcac gttcgaggga cgcgaagtcc tcggcatgcg tctcaactgg
    12421 agcaagcgct acatcacgct ggctcccgcg gcgacggtgc tcgggctcgc cttccgcctt
    12481 cgcgaccccg acaagctgct cggcggccag gaagatctcg ggatcacgtg cgcgctcctg
    12541 ccggtccgcc tcccgggcgt gaagatcggc gagcggcatg atccgctcgg agtgccgttc
    12601 ctgaacgggc ccacgttcgg cacggatgtc ttcgccccgc tcgacttcat catcggcggc
    12661 cccgcgaacg cgggaaaggg ctggcggatg ctcatggact gcctcgccgc gggccgcggc
    12721 atctcgcttc cgtccctctc ggtgggcgcc gcggagctcg cgacgcgcac gacgagcgcg
    12781 tacgccggcg tgcgcgagca gttcggcctc cccatcggac gcttcgaagg catcgaggag
    12841 ccgctcgcgc gcatcgcggg cttgacgtac atgctggatg ccgcgcgcaa gctcaccgcc
    12901 ggtgcgatcg acgcgggcga gaagccggcc gtgatctcgg cgatcgtgaa gcggtacgcc
    12961 accgagggca tgcgcgcggt cgtgaacgac gcgatggata tccaggccgg cgcggcgatc
    13021 agccgcgggc cgcgcaacat cctctcatcg ccgtacgcgt cggtgccggt cgggatcacg
    13081 gtcgaggggg cgaacatcct cacgcggtcg ctcatcgtgt acggtcaggg cgcgatccgc
    13141 tgccatccgt acgtgcagtc ggagatgcgc gccgtggcgg agaaggatct cgtcgcgttc
    13201 gatcgcgcgt tcttcggaca cgtggggcac acgatcggaa ccggattccg cgcgttcggg
    13261 atggcgatct tcggcggcgg cacgggcggc tccggcgtga gcggcgccgc ggggccgatg
    13321 ttcggaaagc tctcgcgcta cgccgcggcc ttcgcgttga tttcggacgc ggcgatggcc
    13381 acgctcggcg gcacgctgaa gcggcgcgag cggctgagcg gccggctggc cgatgcgctc
    13441 gcatggatgt acctcgcgtc cgcgatgctc aagcgcttcg tcgatgacgg ccagccgaag
    13501 cgcgacctgc cgtatgcgcg ctgggcggcg gcgcacgcct tccaccagat cgaggtcgcg
    13561 ctgacgggtg tgctcgagaa ccttccgagc cggcccaccg cgtggatgct ccgccttgcc
    13621 gcgttcccgc tcggcgcgca cgtgcgcccc gtgcccgatc gtgaggtggc ggccgtcgcg
    13681 cacagcgtgc tgaacgacgc cgaagcgcgc gagcatctca ccgccgacat cttcgttccg
    13741 ccgccgggcg agccggggct tggggcgctc gaggctgcct atcgcctgtc tctggcggcc
    13801 gcccccgccg agaagaagct caaggatgcg gtccgcgcgc ggaagctcaa gccgctcgac
    13861 ggctgcgcgc tcttcgacgc cggagtcgag gcgggcctga tctcggacgc cgagcgccgg
    13921 cagctctgcg aggcgctcaa ggcccgcgag gacgtgatcc aggtggacgc cttcggggcg
    13981 aaggagtacg ccgagcagag gcggtaagtc cgaacagacg ccgtccggag aacgtcctac
    14041 cgaagcagct gctcccgcgc catcgccagc cctagcaccg ccccctcatt cccaaccgcc
    14101 ttcgcccgcg ccgcggcgtc cgccaagatc cccgccgcct tcgcgcgatc cgacgtgatc
    14161 gacgccgcga gatcagccgc cacgtccggc gaccaggcca tctcgccttc cgacgtcgcg
    14221 cccgccagga gatcgaacgg cgccttgtgc tccagccagc cgttgtcgtc ggtgttcacc
    14281 ggaccgccgt cggtctctcg cgccacgcgc tccaaccgtc cgcggtagta gcgcatgagg
    14341 tcgggcagcg acgacacgcc gacgcgcgca agatccctgg ccaccttctc gttctggaac
    14401 cacgccccga gctgatccag gtcgagtccg atctcgcgat cgggcgaggc gaggatcacg
    14461 agatcggcgg acttatccac gaagaagagc tgccctttgg gaaaccgcgt cgcgagcgtg
    14521 cggaagatcg tgcggagcgt ggccggggac gtctgatagc actgcaccca ctggcagaag
    14581 atgccgcccg gcctcagctt cgacgccgcg gtctcgtaga agtcgcgcgt gaagaggtcg
    14641 gccatgccgg cgatccagag attcgacggc tcggagatga tgacgtcgta cttgttgcgc
    14701 gtggaccgga ggtacgtgcc cgcgtcctcg atcagcagtc gatgcctcgg atccgcgaga
    14761 ggcgttccgg cgtccgcatc gaagtagggc gcggcttccg ccacggcgcc ttccagctcg
    14821 atggtgagca cgtcgcgcac gggatgcgtg agcaccgcgc cggtcgtgac gccgcttccg
    14881 taacccacga tgcagaccga ctgcgcctga ttcgcgagca gcaccgggag ctgtccgacc
    14941 atcacctgct gcgccatgtc ctcgccgaca ccggttgttg cgtcggtttt tccgttgatg
    15001 aggagcgcgc ggcgctttcc ggtctcgacc accgtcacgg ccgcgacgcg cccctccttg
    15061 tagaagagga ggcgctccag ctccttcgat gcgcccacct ggtcgatcat ccggtgcgtc
    15121 acgtcctcct tgccatacag ccagtacgac cgtaggagcc gcaccaggcc gagactcatc
    15181 cgctccgcgt tccacgacgg catcccgacc cacgcgtacg ctcccgccgc gatcagaacc
    15241 agcagcgcgg ccgcgcggcg gggcagcgcg cgcccctcgc ccgtgaccaa agccaggaca
    15301 ccgagcgctg cgttgagcgc cgctgccgcg aggatcgaat ggagcgtgcc gagccacggg
    15361 atgaggatga aggcggtgag gatcgatccc gcgatcgagc cgatggtgtt gaaggcgtac
    15421 accgcgccca ccgatgcgcc cgcttcgctc cgtgcggtgc ggcggatcgc gacggccgtc
    15481 gggaagagcg cgccgaggag aaagcatgcg gggaagatga tccacgccgt gatccccgcc
    15541 tccccccaga ggagcgagga cgtgcttccg ttcgcgtgca ccatgaccgt cgtctgccag
    15601 tgggggagat gctcgaattg atgcgccgcg atcccgaccg cggcagcgag caacgcctgc
    15661 aggattccaa agagcgtgac cggcgcgcgc cacagcttca aaacccgcga tgcggtcgcc
    15721 gcgccgagcg cgatgcccgc gagatagatc gcgaggatgg ccgagaacgc gtagatcatg
    15781 ccgccgaagg gcacggcgag gatgcgcgtc caggagactt cgtacaggat cgcggtgaat
    15841 ccggagagcg caaacaaggg aagaatccag cggtgccacg ggggagccgc cgcgccgtca
    15901 tcccggacca ccgcggccgc tcccgcacca tccgccgcca cgccaggcgg cgcggccgga
    15961 cgtccgaaaa ggatcgtcgc aaacacgatg agatcgatcg ccgcggcgac cgcggccgtc
    16021 ttccagagac cgagcgccgg gatcgcgagg aatccggccg cgacgacgcc aagcaccgcg
    16081 cccgctgtgt tcacggcata gaggcgcgcc accgcggctc ccgatccctc gcgatcgcgg
    16141 cgcgagaagt cggatacgag gagcggcagc gtcgctccca tcgcggtggt aggaatgagg
    16201 agcgcagcga agaggaccag gaatcgcaga agcaagaaaa cggcgccgtg gccgagaaag
    16261 agtccgttca gccacgcgaa cagcggcttc gcgagcgcga atcccagcgg actcatcatg
    16321 ccgagcaccg caaccaccag ctcgagtcgc gcgtacagca acaagggctc gcgcgttcgc
    16381 gcggcccagc gtcccccgat cgccgctccg atgccgagcc cgcccaggaa cgccgcgacc
    16441 acggtcgaga cgccgtagat cgaattcccg aggaggagcg agaggtactt ggaccagacg
    16501 atctcgagga tcagtccggc cgccccggag gcgaaaaaac aggcgtagag gagcggccgt
    16561 cgcgatcccg gacgcgccgc caagtcctga tgctttctcg cgctagacgc tgccgccgtt
    16621 cgccgcgagg cggcgcggtt tcccttggac tgggtcatgg aatgtggtat agtacctctt
    16681 tccacgcgcg ggtcatgggc ccacgcaccg aatcgtagaa agcattcccc agttttctcg
    16741 agacacagtg cggagacccg ctcccttagc tcgacaccac gcttccgtgt ccgcaggcat
    16801 tcctctccgt ctcgcggtca cgggctttct gaccctcctc tggatcgctt tcgaggcggc
    16861 ccgcgcattc tctgcgccgt cagccacgaa tcccgtcgat cagtggccgc gccgcgtcta
    16921 ccgcaccgag gttcccgctc ccctcaaagg cgatccgtcc gctccgatgt cgtaccggcg
    16981 cgtcctgctc accccgccgc cgctggaaga ggtgtatgtc ttcaacgaca gcctggacgg
    17041 gcacccgctc gacgacgagg ggggctggag ccacttcgac aattccgccg gaccgaccgc
    17101 ctggcacatc gacaccttca tggcgtgcaa caaccacgcg tggtggtgcg gcatggtcga
    17161 ttcgagctgg atcaacgaca cgaaccgtgc gggatacgac aacagctgga cccagtacct
    17221 ccagcacgac gcgccgacgg cgaccgtgcc cgccggctcg atcatcacgc tgaccgcgaa
    17281 gtacaagatc aacgtcgagc agggctatga ctacggcgag gtggagtaca acgacctgga
    17341 tgacctctgg ctcccgctcc tgcacctgac gggcaagatc ccggccagcg gcgtgggctg
    17401 cgattcgttc acggtggagc tgccggagtc gagctgggtg aagtggcaga accatccgac
    17461 cggcgccaag cccatgccct tccgcttcgt cttccactcc gacatcgagc gatcgtcggc
    17521 cgacggcctc tacaacggag acggctggat catcgacgag gtccgggtca cgaaggacgg
    17581 caaccagatc ctgttctacg acaacatgga gaacgggatg gggacctgga cccggacgac
    17641 gctcccggcc gtgggcgact actacgcgtt ggagaacaac gtcgtcactg aagatcaatg
    17701 caccgacaac cgcacgaaca tctgggtgga ctgggaccag gtcacccgaa gtctcgtgcc
    17761 ccggatcgac gaccgcctgc tgacaccgcc cgtcgacatc gatcggtcgg gtgaggtcct
    17821 gatcgccttc gacgtgtacc gaaatctccc gctcaatgcg tgcttctact attccgtgaa
    17881 ctatcgaacg cggatgatcg ggggcaactg gaccgcgtgg atggatccca ccgaccttct
    17941 ctactacggc tccagcaagg actggatccg tcagaagatc gtgctgccgc tggccgccgg
    18001 gaaggattcg gtccagtgca tgttcatcgt gaaggactat ggccagatct actgcggcgg
    18061 gtcgaccggc tactcgaacc tgtacccgct cttcgacaat atcgccattg gcgtgcgcgc
    18121 gaccacgcct cccatcttca ttccgcgcga tctcgacctc ttccaggaca cgttcaagac
    18181 cacggcgttc ttcggcaacg acaacttcaa taccccgctc ggcgacagc
//
LOCUS       DASTKC010000205         7380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_875309, whole genome shotgun sequence.
ACCESSION   DASTKC010000205 DASTKC010000000
VERSION     DASTKC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875309"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFR25_08560"
     CDS             complement(<1..712)
                     /locus_tag="VFR25_08560"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET9941143.1"
                     /translation="MSWTPPDHHPTSWILVRREQGGTFAPVATVVGTDSHYSDDTAQN
                     GTAYEYQLVASPADPSLDSEVSGPARASVNFFDWSKFNVLVVAGIFFALLFWYIRKSE
                     GGVKWVFRTIPGLAAIEEAVGRATEMGKGVLYVPGVQEIDDIQTIASMILLGRVARIA
                     AKYETDLNVPTNSPGVYTVAEEVVKSAYADVGRTDAYKSDQVRYVTSEQFAYVAAVNG
                     MMLRERPAANLLLGAFFAE"
     gene            complement(1025..2152)
                     /locus_tag="VFR25_08565"
     CDS             complement(1025..2152)
                     /locus_tag="VFR25_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941144.1"
                     /translation="MAHSYTPGLKVARFTVLRRERKLPLAGDVLVKVGQKVEAETVVA
                     RTELPGNVQTVNAANILGVLPEDVPDCLVKPVGSTLKKGEAFAESKSFFGLFKSKCMA
                     PIDSTLESISTVTGQAILREPPIPVEVDAYVDGTVVEVHESQGVTVETRGSFIQGIFG
                     VGGETVGALHVCVDGPGDVLTEAKIGDDVRGKILVGGSHVSTKVLRAAIQKGARAVVV
                     GGFDDHDLREFLGYDLGVAITGSEEKGITLVVTEGFGEIPMAGRTFELLKQCEGKKAS
                     VSGATQIRAGVLRPEIVVPQEISAGTGAGGDGADHHGLEVGSLIRVIREPFFGRLGKV
                     VTLPPELMALETEAKVRVLEVDFGNGSKSLLPRANVEMIES"
     gene            complement(2156..3976)
                     /locus_tag="VFR25_08570"
     CDS             complement(2156..3976)
                     /locus_tag="VFR25_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HET9941145.1"
                     /translation="MSIPKPDDIQSILATDCGSTTTKAILIERRGAEYRLIVRGEAPT
                     TVEAPFEDVTRGVLNAIMEVEELAGRKLLDGERILSPQKGNEGVDLYISTSSAGGGLQ
                     MMVSGVVKSMTGESAQRAALGAGAIVMDVIASNDGRLPHEKIKRIRQLRPDMILLSGG
                     IDGGTTSHVAEMAELLAAADPKARLGAGYELPVIYAGNKDARPIVEERLASKTALVVT
                     DNLRPVLERENLSPARDKIHDLFMEHVMAHAPGYKKLMTWSPVPIMPTPGAVGLIMER
                     IARDEKINVIGVDIGGATTDVFSVFQGIFNRTVSANLGMSYSVSNVLAEAGLPNILRW
                     VPFEIGEPDLRNRIKNKMIRPTTIPQTLEELVIEQAIAREALRLAFEQHKMLAVGLKG
                     VQQERTISDTFEQTGSGATLVRLDHLDLLVGSGGVLSHAPRRAQSALMMLDAFLPEHV
                     TELAVDSIFMMPQLGVLSTVHPEAAKQVFEKDCLIRLGASVAPVGPCKDGVTALKASL
                     EFQGGRKEEVSVPFGYLRLIPVPDGQTVKAMLRPDRGLDVGAGKGKEREVTLRGGVVG
                     VILDCRGRQPFVLPQDRTQRITKLAEWNEALDIYPVLARV"
     gene            complement(3973..4287)
                     /locus_tag="VFR25_08575"
     CDS             complement(3973..4287)
                     /locus_tag="VFR25_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941146.1"
                     /translation="MLDRVAERVVALRMTVPAILTLESVKPLSFVSGQALYFFEPMVR
                     AFFAVPDYERFAALVERRDTLEALLVKIEQRDEAVRDEERAAKARRRAERDQARNRKE
                     PS"
     gene            complement(4313..4765)
                     /locus_tag="VFR25_08580"
     CDS             complement(4313..4765)
                     /locus_tag="VFR25_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941147.1"
                     /translation="MPEAERERRPAGTPEGALTWTAWPARERPLAAAVLAASAIVLGV
                     LVAHGTQDRVLGVATPLFILVSLSSFLLPTTYRLTDDAVEVRSLGVARVRPWTEMRRM
                     TVDKTGVFLSPFEKRSWLEAYRGVRLLFGGNRDQVVAFVEARLKSRPA"
     gene            complement(4752..6221)
                     /gene="gcvPB"
                     /locus_tag="VFR25_08585"
     CDS             complement(4752..6221)
                     /gene="gcvPB"
                     /locus_tag="VFR25_08585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9941148.1"
                     /translation="MSELTLSELSVDGHRGPRLMPLDVPARGVDRLLPADRVRKDRAA
                     LPEISEPVAMRHFVNLSTLNHHIDKAIYPLGSCTMKYNPKLNDEMARIPGFAGIHPLQ
                     PDELAQGALAVVAELHDWLRVVMGMPGVTTQPVAGAQCELTAVLMARAYHRDHGDPRE
                     EVIVPDSAHGTNPSTVHQVGYRVVQIPSGPDGCIDLDALKRAISPKTAAMMLTNPNTL
                     GIFERQVGTIAKLIHDAGALLYLDGANLNAMMGLASPGAMGFDFAHINLHKTFSTPHG
                     GGGPGGGVLCVRKDLEPYLPMPVVEQDGDRYRWSYDRPKSIGRVHSYYGNFGILVRAL
                     CYMRSLGASGIAEASREAILNANYLKARLKDAYAPSHEGPCMHEFVLSASRQKARGVK
                     ALDIAKRLLDFGMHAPTIYFPLIVEEALMIEPTETETKASLDAFADAMLQIDRESTED
                     PNRLRQAPTQTPVRRLDEGRAARKLILSWQDLEAEGAGG"
     gene            complement(6209..>7380)
                     /gene="gcvPA"
                     /locus_tag="VFR25_08590"
     CDS             complement(6209..>7380)
                     /gene="gcvPA"
                     /locus_tag="VFR25_08590"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9941149.1"
                     /translation="GGGIYDHFIPAALRSIVSRSEYATAYTPYQPEVAQGTLQVIFEY
                     QSMIRELTGMEVANASLYDGATAAVEAAHFAAGATGKKRVLCSAGVHPHTREVLRTYA
                     SAGGFQLEEIALENGRTPVAPIAKAGSDVAAVIWAQPNFEGVVEDGAALTQAAHEIGA
                     YSLAGADPVALAVLRPPGHDGADVVFGEGQPLGVPMAYGGPLVGFFAIRKAEVRRLPG
                     RLAGVTVDLEGKRGYVLTLQTREQHIRRERATSNICTNQGLMALANTVYLALLGAEGL
                     KDVATQCLERAHYLADRVREIPGIALAHKDAPFFRELVLRLPAPAESFARAALDRRIL
                     AGIPLARFDRSRPNDLLVAVTEKRSREDLDRYVETLASWAKAHANSPREEAACQS"
BASE COUNT         1127 a         2564 c         2484 g         1205 t
ORIGIN      
        1 attccgcgaa gaaggctccg aggaggagat tcgccgcggg ccgctcgcgg agcatcatgc
       61 cgttgaccgc ggccacgtag gcgaactgct ccgaggtgac gtagcggacc tggtcgctct
      121 tgtaggcgtc cgtgcgcccc acgtcggcgt acgccgactt cacgacctcc tcggcgacgg
      181 tgtagacgcc cggggagttc gtggggacgt tcaggtcggt ctcgtacttg gcggcaatcc
      241 gcgccacgcg cccgaggagg atcatgctcg cgatggtctg gatgtcgtcg atttcctgca
      301 cgccgggcac gtagagaaca cccttcccca tctccgtcgc gcggccaacc gcctcctcga
      361 tcgcggcgag acccgggatc gtgcggaata cccacttcac cccgccttcc gacttccgga
      421 tgtaccagaa gaggagcgcg aagaagatcc ccgcgaccac gagcacgttg aacttgctcc
      481 aatcgaagaa attcaccgac gcgcgcgcag ggcccgagac ctcggaatcg agggagggat
      541 cggcgggcga ggccacgagc tggtactcgt aggcggtgcc gttctgcgcc gtgtcgtccg
      601 agtagtgcga gtcggttccg acaaccgtcg cgaccggcgc gaacgttccc ccctgctcgc
      661 gtcggacgag gatccaggag gtcggatggt gatccggtgg cgtccaggac acgtcgacgc
      721 tgccaccccc gtcgttggga gtgtcgccaa ccttgagcgg gaatgcggag gcgagggtcg
      781 ggagggccag gatcgtaaga gtggtgagga ggaacgcggc taccgcgaac cgagggagtc
      841 ggtcgccggg agctcgtagc aggatccgcg cgtcgcggag atttgtcagg aagtagctcc
      901 cgtatgggtg cgaagcgctc catccctgcc gcgatcgaga ctcagcacgt ggcgcgtttc
      961 ctcccaggta ccgaccggca aaaccgcaac aaccgtagac tccggcgccg cttctgtcaa
     1021 gaagctagga ctcgatcatc tccacgttgg cgcgcggaag gagcgacttc gatccgttcc
     1081 cgaaatccac ctcgagcacg cgcaccttgg cctctgtctc gagagccatc agctcgggcg
     1141 gcagcgtgac caccttcccg aggcgcccga agaagggctc acggatgacg cggatcaagc
     1201 ttcccacctc gaggccgtgg tggtcggctc cgtcgccgcc ggccccggtt cccgccgaga
     1261 tctcctgcgg cacgacgatc tccgggcgca gcacgccggc ccggatctgg gtggctccgc
     1321 tcaccgacgc cttcttcccc tcgcactgct tcaagagctc gaacgtgcgc cccgccatcg
     1381 ggatctcgcc gaagccctcg gtcacgacga gcgtgatgcc cttctcctcc gatcccgtga
     1441 tcgcgacgcc caggtcgtag cccaggaact cgcggagatc gtgatcgtcg aatcccccca
     1501 cgacgaccgc gcgagcgcct ttctggatgg ccgcgcggag caccttggtg ctcacgtgcg
     1561 agccgcccac gaggatcttc ccgcggacgt cgtcgccgat cttggcctcg gtgagaacat
     1621 cgcccggacc gtccacgcac acgtggagcg ccccgacggt ctcgccgccg acgccgaaga
     1681 tcccctggat gaagctccct cgggtctcca ccgtcacgcc ctggctctcg tgcacttcga
     1741 cgaccgtgcc gtcgacgtac gcgtccacct ccacggggat cggaggctcg cgcaggatgg
     1801 cctgcccggt cacggtcgag atgctctcga gcgtcgaatc gatcggcgcc atgcacttcg
     1861 acttgaagag tccgaagaac gacttgctct cggcgaaagc ctcccccttc ttcagggtcg
     1921 agccgaccgg cttgacgagg caatccggga cgtcctcggg aagcacgccg aggatgttcg
     1981 ccgcgttcac cgtctgcacg ttcccaggca gctccgtgcg cgcgacgacc gtctccgcct
     2041 ccaccttctg ccccaccttc acgagcacgt cccccgcgag cggaagcttc cgctcccggc
     2101 ggagcacggt gaagcgggcg accttcagac cgggggtgta ggagtgagcc atgggtcaga
     2161 ccctcgccag gaccgggtag atgtcgaggg cctcgttcca ctcggccagc ttggtgatcc
     2221 tctgggttcg atcctgcggg agcacgaagg gttggcggcc gcggcagtcc aggatcacgc
     2281 cgacgacccc gccccggagc gtcacctcgc gctccttccc cttgccggcg cccacgtcga
     2341 ggccgcggtc gggccggagc atcgccttca cggtctggcc gtcgggaacc gggatgagcc
     2401 tcaggtagcc gaacggcacc gagacctcct ccttccgccc gccctggaac tcgagcgacg
     2461 ccttgagcgc cgtcacgcca tccttgcacg ggcccaccgg agcgacgctc gcgccgagcc
     2521 ggatcaggca gtccttctcg aacacctgct tcgccgcctc gggatgaacc gtcgagagga
     2581 cgccgagctg cggcatcatg aagatcgagt cgaccgcgag ctccgtgacg tgctcgggga
     2641 ggaacgcgtc gagcatcatg agcgccgact gcgcgcgcct gggcgcgtgc gagaggacgc
     2701 cgccgcttcc cacgaggaga tcgaggtggt ccaggcgcac gagcgtcgcg ccgctcccgg
     2761 tctgctcgaa ggtgtcggag atcgtccgtt cctgctgcac gcccttcaga ccgacggcga
     2821 gcatcttgtg ctgttcgaac gcgagccgga gcgcctcgcg cgcgatcgcc tgctcgatca
     2881 cgagctcctc cagcgtctgc ggaatcgtgg tcgggcggat catcttgttc ttgatccggt
     2941 tccttagatc cggctcgccg atctcgaacg gcacccagcg gaggatgttc ggaaggccgg
     3001 cttcggcgag cacgttcgac acgctgtagg acatgccgag gttcgcgctc acggtgcggt
     3061 tgaagatccc ctggaagaca gagaagacgt cggtcgtggc cccgccgatg tcgacgccga
     3121 tgacgttgat cttctcgtcc cgcgcgattc gctccatgat gagcccgacc gcgccggggg
     3181 tcggcatgat cggcaccggc gaccaggtca tgagcttctt gtacccgggc gcgtgcgcca
     3241 tcacgtgctc catgaagaga tcgtggatct tgtcgcgggc gggcgagaga ttctcgcgct
     3301 cgagaaccgg acggaggttg tcggtcacga cgagcgcggt cttcgaggcg agccgctcct
     3361 cgacgatcgg gcgcgcgtcc ttgttccctg cgtagatcac cgggagctcg tagcccgcgc
     3421 cgagccgcgc cttgggatcg gccgcggcca ggagctcggc catctcggcc acgtgcgagg
     3481 tggtgccgcc gtcaatcccc cccgaaagga ggatcatgtc ggggcggagc tgtcggatgc
     3541 gcttgatctt ctcgtgcggg agccgcccgt cgttgctggc gatcacgtcc atgacgatcg
     3601 cgcctgcgcc gagcgccgcg cgctgcgcgc tctcgcccgt catgctcttc acgacgcccg
     3661 agaccatcat ctggagcccg ccgccggcgc tcgaggtcga gatgtagaga tcgacccctt
     3721 cgttgccctt ctgcggcgag aggatcctct cgccgtcgag gagttttcgg ccggccagct
     3781 cctcgacctc catgatggcg ttcagcacgc cgcgcgtgac gtcctcgaac ggcgcttcca
     3841 ccgtggtcgg cgcttcgccg cgcacgatga ggcgatactc cgcgccccgg cgctcgatca
     3901 ggatcgcctt ggtggtcgtg ctgccgcagt cggtcgcgag gatcgactgg atatcgtcgg
     3961 gcttgggaat gctcacgaag gttcctttcg gttccgcgcc tggtcgcgct ccgcgcgccg
     4021 gcgcgccttg gccgcccgct cctcgtcgcg aaccgcctcg tcgcgctgct cgatcttcac
     4081 gagaagcgct tccagggtgt cgcgccgctc cacgagggcc gcgaatcgct cgtagtccgg
     4141 aaccgcgaaa aaggcccgaa ccatcggttc gaaaaagtag agcgcctggc cgctcacgaa
     4201 cgagagcggc ttgacgctct cgagcgtgag aatggccggc accgtcatcc ggagcgcgac
     4261 cacccgctcc gccacccggt cgagcaacgc ctcgtcttcc ggcgaccgcg tctcaggcgg
     4321 ggcgggactt gaggcgcgct tccacgaacg caaccacttg atcgcggttc cctccaaaga
     4381 ggagccggac gccgcgatag gcttcgagcc agctccgctt ctcgaacggg ctcaggaaaa
     4441 cgcccgtctt gtccaccgtc atgcgccgca tctccgtcca cggccggact cgcgccacgc
     4501 ccagggaccg cacctcgacc gcgtcgtcgg tcagccggta cgtcgtcggc aagagaaacg
     4561 acgagagcga gacgaggatg aagagcggcg tcgccacgcc cagcacccga tcctgggttc
     4621 cgtgcgccac gagcacgccg aggacgatcg cggaggccgc gagcacggcc gccgcgagcg
     4681 gccgctcgcg cgcgggccac gcggtccagg tgagcgcgcc ttccggcgtt ccggccgggc
     4741 ggcgctcccg ctcagcctcc ggcaccctcg gcctcgagat cctgccagga gaggatcagc
     4801 ttcctcgcgg cgcgcccctc gtccaggcgg cgcaccggcg tctgcgtggg cgcctggcgc
     4861 aggcggttcg gatcctccgt gctctcccgg tcgatctgga gcatcgcgtc cgcgaaggcg
     4921 tcgaggctcg ctttggtctc cgtctcggtg ggctcgatca tgagcgcctc ctcgacgatg
     4981 agcgggaagt agatcgtggg cgcgtgcatg ccgaaatcga ggagccgctt cgcgatgtcg
     5041 agtgccttca ccccgcgcgc cttctggcgc gacgccgaga gcacgaactc gtgcatgcag
     5101 ggtccttcgt gagacggcgc gtacgcgtcc ttgagcctcg ccttgagata gttcgcgttc
     5161 aggatggcct cgcggctcgc ctcggcgatg cccgacgctc cgagcgagcg catgtagcag
     5221 agcgcccgca ccaggattcc aaagttcccg taatacgagt gcacgcgccc gatcgacttc
     5281 ggccggtcgt agctccagcg gtaccggtcg ccgtcctgct ccaccaccgg catcgggaga
     5341 tacggctcga ggtccttccg cacgcagaga acgccgccgc cggggcctcc cccgccgtgc
     5401 ggcgtcgaga acgtcttgtg aagattgatg tgcgcgaagt cgaagcccat cgcgcccggg
     5461 ctggcgagcc ccatcatcgc gttcaggttc gcgccgtcca gatagaggag cgcgccggcg
     5521 tcgtggatga gcttcgcgat cgtcccgacc tgccgctcga agattccgag cgtgttcggg
     5581 ttggtcagca tcatcgccgc cgtcttgggc gagatcgcgc gcttcaaggc gtcgaggtcg
     5641 atgcaaccgt caggacccga tgggatctgg accacccggt agcccacctg gtgcaccgtc
     5701 gacgggttcg tgccgtgcgc cgagtcgggc acgatcactt cctcgcgcgg gtcgccgtga
     5761 tcgcggtggt acgcccgtgc catgagcacc gcggtcaatt cgcactgcgc gccggcgacc
     5821 ggctgcgtgg tcacgcccgg cattcccatc acgacgcgga gccagtcgtg gagctccgcg
     5881 acgacggcga gcgctccctg cgccagctcg tccggctgga gcggatggat ccctgcgaag
     5941 cccgggattc gcgccatctc gtcgttcagc ttcgggttgt atttcatcgt gcacgacccg
     6001 agcggataga tggccttatc gatatggtgg ttcaaggtcg agagattcac gaagtggcgc
     6061 atcgcgaccg gctcggagat ctcggggagc gcggcgcggt ccttccggac gcggtcggcc
     6121 ggcagcagac gatcgacgcc gcgagcgggt acgtcgagcg gcatgagccg cggcccgcgg
     6181 tgtccgtcga cggagagctc gctcagggtc agctctgaca cgcggcctcc tccctcgggc
     6241 tgttggcgtg ggccttggcc cacgaggcga gcgtttcgac gtagcggtcg aggtcttccc
     6301 tgctccgctt ctcggtcacg gcgacgagga gatcgttcgg acgggagcgg tcgaagcggg
     6361 cgagcggtat gcccgcgagg atgcggcggt cgagcgccgc gcgggcgaac gactccgcgg
     6421 gcgcgggaag gcgaagcacg agctcccgga agaagggcgc gtccttgtgc gcgagcgcga
     6481 tgcctgggat ctcgcggacc cgatcggcca ggtagtgcgc gcgctccagg cactgggtcg
     6541 ccacgtcctt gagcccctcc gcgccgagga gtgccagata gaccgtgttc gcgagggcca
     6601 tcagcccctg gttcgtgcag atgttcgaag tcgcgcgctc ccgccggatg tgctgctcgc
     6661 gggtctggag cgtgagcacg taaccgcgct tcccctcgag atccacggtc acgccggcga
     6721 gacgccccgg gagccgcctc acctccgcct tccggatggc aaagaagccg acgagggggc
     6781 cgccgtaggc catcggaacg ccgagcggct gcccttcgcc gaagaccacg tccgctccgt
     6841 cgtgccccgg agggcgcagc acggcgagcg ccaccggatc cgctcccgcg agcgaatagg
     6901 cgccgatctc gtgcgcggcc tgggtgagcg cggcgccgtc ttccacgacg ccctcgaagt
     6961 tcggctgcgc ccagatcacg gccgccacgt cggacccggc cttggcgatc ggtgcgacgg
     7021 gcgtgcgccc gttctcgagc gcgatctcct cgagctggaa tccgcccgcc gacgcgtagg
     7081 tgcggagcac ctcgcgcgtg tgcgggtgga ctccggccga gcagagcacg cgcttcttgc
     7141 ccgtggcgcc ggccgcgaag tgggctgctt ccacggccgc cgtcgcgccg tcgtacaggg
     7201 acgcgttggc gacctccatg ccggtcagct cgcggatcat ggactggtac tcgaagatca
     7261 cctggagcgt gccctgcgcg acctcgggct ggtatggcgt gtaggccgtc gcatactcgg
     7321 agcgggacac gatcgagcgg agcgcggccg ggatgaaatg gtcgtagatg ccgccgccca
//
LOCUS       DASTKC010000206         5731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2059396, whole genome shotgun sequence.
ACCESSION   DASTKC010000206 DASTKC010000000
VERSION     DASTKC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059396"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFR25_08595"
     CDS             complement(<1..451)
                     /locus_tag="VFR25_08595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9941150.1"
                     /translation="MTDLSHPVLYASPEIYDVAFGWDLALELDFLESCFTAHVDGPVR
                     RILEPACGTGRLLEALAGRGYDVLGYDLSPEMVAFASRRLGAHGGRALRGDMATFRPP
                     GRFDVAINPVNSIGYLLEDEQLLQHFERIAEALRPGGIYIAQFNYWGE"
     gene            complement(578..778)
                     /locus_tag="VFR25_08600"
     CDS             complement(578..778)
                     /locus_tag="VFR25_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9941151.1"
                     /translation="MASGTVKWFNEAKGFGFIQQEGGEDVFVHYSAIQGDGFKTLAEG
                     ERLEFDIVRGPKGLQAANVRRA"
     gene            1039..2097
                     /locus_tag="VFR25_08605"
     CDS             1039..2097
                     /locus_tag="VFR25_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET9941152.1"
                     /translation="MFLPSGPEGPVEETAVASSLPAVEHVPEFIDQPLATLGLRIEGS
                     PVEPFLHRLAGELKAKGIRRWHPRYYLTDEWGCPSGHPIIGIPFYLADKRLARIERER
                     NELESPREVMMYLRHEAGHAFNYAYRLYQTAEWRATFGPFRRRYREDYRPVPFSRDFV
                     RHLPGWYAQKHPDEDFAEAFAVWLTPGYPWRERYAGWPALQKLEYVDRTVRACGDREP
                     VVKRARPDITVEEMEGTVREVFDEANARNRSAMELNPDDELVDIFLPRGSRRKPTRPA
                     WEIVEQHRNKLIDKIAHWTGVRRPVVREMVDSIVEACRARGYLGLRGKESDYLVALTA
                     YGTAVAMNYMTRGKFRNV"
     gene            2117..3175
                     /locus_tag="VFR25_08610"
     CDS             2117..3175
                     /locus_tag="VFR25_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET9941153.1"
                     /translation="MTENPLTICILYDAWHELEQEAPEPPPENPKRARKRREKSDREE
                     IAEALTKRGHTVIEHCLDGRRRSLKALAGIEADLVFNLTESYGGDDTKDLNIAAYLDL
                     LHLPYTGSGPSGLLLAQDKAVAKRLFTFHGLRTPDFATVYQGRLQWSDDIHFPVIVKP
                     KREDGSIGIEFSAVVGSIKELMERIDALHADLDSPVLIEEYIEGRELYVSVLGNEPPT
                     ALPVVELDLSDLPEGTPRIAGTEVKWERGTDAYKKTKLRVPEDLGEEVMAEIQETAVT
                     ACRALEIRDYARADLRLTPDGRIFLLEINPNPWLHSTAEFALSAKQAGRDYGSLIEEI
                     VAMAIARYRGRTLSSRPD"
     gene            3342..>5731
                     /locus_tag="VFR25_08615"
     CDS             3342..>5731
                     /locus_tag="VFR25_08615"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9941154.1"
                     /translation="MSHTQSKHRAHLRAPLALLALIALALAPAVASAGVKPPQVTLNY
                     EKYTLPNGLEVILYEDHKLPLVAVNTWYHVGPANELPGRTGFAHLFEHMMFQSSGHVG
                     EDQIWKYLEGAGASFINGTTDFDRTNYMEDMPSNQLETAIWLESDRMGYLLDTLTGPS
                     LANQQDVVRNERRQSIENAPYQIVEEEMWHMLFPKGHPYYAWIIGSHEDVQAAKLDDV
                     KNFFRSYYAPNNASLVIAGDIDKAKVKALVEKYYGSIPKGEPVPAITATTPPITAERR
                     ATVTDKITLPRVYMAWLTAPILKPGDAEAGVAADILGGGKASRLYKSLVYTKRICQDV
                     SVVQQSYALSSVFQISATVKPGHTPDEVEKAINEELATFIQNGPTDAEVTATQNALYS
                     NIVTSLENLGGFSGIADRMNSYNQAVKDPGYLSKDLARFEAVTPAAVKQFAAQSLRND
                     ARVVVYAVPGDKKLPPDPPAPPPPSKEEAKVESKEPWRNTPPAAGAENQAKLPTPKKI
                     ALPNGLTVYVVESHRFPLVSANLVLRSGSAADPADLPGLAGYTAAMLDEGTTKRDALA
                     LADQTHALGASLVTGSQPDGSSAGIRSLKQNAAAAIDILSDVTLHPSFPDQEIERIRN
                     DRLTTLMQQRDQPWPTGIRVMNACLFGPAHPYGHTQIGTQASIEKTKRDDLVKFYGST
                     YSPKDAALILVGDLTEAEAKKLATDAFGAWKGAATPAPVPPTGTMANSRIVIVDKPGS
                     VQTVVLAGQLGVKRSDPDYEKLDLMNTVLGGLFSSRINLNLREDKGYSYGAFSFVG"
BASE COUNT         1065 a         1967 c         1850 g          849 t
ORIGIN      
        1 gctcgcccca gtagttgaac tgcgcgatgt agatcccgcc cggacgcagc gcctccgcga
       61 tgcgttcgaa gtgctggagc agctgctcgt cctcgagcag gtagccgatc gagttcacgg
      121 gattgatggc gacgtcgaag cgccccggcg gacggaacgt ggccatgtcc ccgcgaagcg
      181 cgcgcccgcc gtgcgcgccc aggcggcgcg acgcgaaggc caccatttcg ggactcaaat
      241 cgtatccaag gacgtcgtag ccgcgccccg cgagcgcctc caagaggcgt cccgtgccgc
      301 acgccggctc gaggatgcgg cgcaccggtc cgtccacgtg cgcggtgaag caggattcga
      361 ggaagtcgag ctcgagcgca agatcccagc cgaaggcgac gtcgtagatc tcggggctcg
      421 cgtagagaac gggatggctc aggtcggtca tggctcctgc gtcatggcga gaacgaaggc
      481 cggcggcgca ccgggcgcgc ggcgccggag gaaaagcaaa cggggcggcg ctggatgcgc
      541 cgccccgtgc ttgacggaag tgccgtggtt cggctgccta cgcccggcgg acgttagcgg
      601 cctggaggcc cttcggccca cgaacgatat cgaactccag acgctcgccc tcggcgagcg
      661 tcttgaagcc atcgccctgg atcgcggaat agtggacgaa cacgtcctcg ccaccttcct
      721 gctggatgaa cccgaaaccc ttcgcctcgt tgaaccactt caccgttcca ctcgccactg
      781 atctgctcct tggactacgg ggtacgaccc caaacacgcc gcgttgcgcg gcaaacgccc
      841 agccaactag gccgggttcc gggcggatcg tgaagccccg accggataca agaaagccgc
      901 aggaatcgtc tctcctgcgg cgacagtcac accatgtgtg cttcaactac cgacaggcaa
      961 cgatcggagt atcggtaaga acccgctgtt tgtaaagcaa aatctttctt cggcggcgag
     1021 ggatgatagc ctgtaaccat gttcctgcca tccggtcccg aaggtcccgt cgaggagacc
     1081 gcggtcgcgt cgtctctacc cgcggtggag cacgtccccg agttcatcga tcagccgctc
     1141 gcgacgctgg ggctcaggat cgagggctct ccggtcgaac ctttcctgca tcggctcgcg
     1201 ggcgagctca aggcgaaggg aatccgccgc tggcatcccc gctactacct cacggacgag
     1261 tggggatgtc cgtcgggcca tccgatcatc ggcattccat tctatctcgc cgacaagaga
     1321 ctcgcgcgaa tcgagcggga gcggaacgag ctcgagagtc cgcgcgaggt gatgatgtac
     1381 ctgcgtcacg aggcgggcca cgcgttcaat tacgcgtatc gcctctacca gaccgccgag
     1441 tggcgtgcta cgttcgggcc gttccggcga cgctatcgtg aagattaccg gcccgttccg
     1501 ttcagccgcg atttcgtgcg ccatctgccc ggctggtacg cgcagaagca tcccgacgag
     1561 gacttcgccg aagcgttcgc ggtgtggctc acaccgggct atccgtggcg ggagcgctac
     1621 gccggatggc ccgcgctcca gaagctcgag tacgtcgatc gcaccgttcg cgcgtgcggg
     1681 gaccgcgagc ccgtcgtcaa gcgggcgcgc cccgacatca ccgtcgaaga gatggaagga
     1741 acggtccgcg aggtcttcga cgaggcgaac gcgcgcaatc gctcggccat ggagctgaac
     1801 ccggacgacg agctggtcga catcttcctt ccgaggggaa gccggcggaa gccgacccgg
     1861 cctgcctggg agatcgtcga gcagcaccga aacaagctca tcgacaagat cgcgcactgg
     1921 accggcgtcc gccggccggt ggtccgcgag atggtggatt cgatcgtcga ggcgtgccgg
     1981 gcccgcggct atctcgggct ccgcgggaag gagagcgact acctggtcgc gctcaccgcg
     2041 tacggcacgg cggtcgcgat gaactacatg acgcgcggca agttccggaa cgtgtgacgg
     2101 cgcacggcga gggcgaatga ccgaaaatcc gctcaccatc tgcatcctgt acgacgcgtg
     2161 gcacgagctc gagcaggaag cgcccgagcc gccgcccgaa aatcccaagc gcgcgcggaa
     2221 gcgacgggag aagagcgatc gcgaggagat cgcggaagcg ctcaccaagc ggggccacac
     2281 ggtgatcgag cattgcctcg acgggcgccg gcgcagcctg aaggcgctcg cgggcatcga
     2341 ggcggatctc gtcttcaact tgaccgaatc ctacggcggc gacgatacga aggacttgaa
     2401 catcgccgcc tatctcgacc tcctccacct cccctacacg gggtcggggc cgtccggact
     2461 cctcctcgcg caggacaagg ccgtggcgaa gcgcctgttc acgtttcacg gactccgcac
     2521 gcccgacttc gccacggtct accaggggcg cctccagtgg tcggacgaca tccacttccc
     2581 ggtcatcgtg aagcccaagc gcgaagacgg ctcgatcggg atcgagttca gcgcggtggt
     2641 cgggtcgatc aaggagctga tggagcggat cgacgcgctc cacgcagacc tggattcccc
     2701 ggtgctcatc gaggagtaca tcgaggggcg cgagctctat gtgtcggtgc tcgggaacga
     2761 gcctccgacc gcgcttccgg tcgtggagct ggatctctcc gacctgcccg aggggacgcc
     2821 ccggatcgcg gggaccgagg tgaagtggga gcgcgggacc gacgcctata agaagacaaa
     2881 gctccgggtc cccgaggacc tgggcgagga ggtgatggcc gagatccagg agacggcggt
     2941 caccgcctgc cgggcgctcg agatccgcga ctatgcgcgg gccgacctcc ggctgacccc
     3001 ggacggacgg atcttcctcc tcgagatcaa tcccaatccc tggctccact cgacggcgga
     3061 attcgccctc tcggccaagc aggccgggcg tgattacggc tccctcatcg aggagatcgt
     3121 ggccatggca atcgcccgct accggggccg caccctgtcg agccgccccg actaggcctc
     3181 cccgctccaa cccgattcct cccaaccctc ccggcgcacc gggcatccaa tggcttgaca
     3241 gggccggagg catgcccaaa actggacccc caattcgatc cgagcacgac ttttcgaccc
     3301 cgcgtccccc acgccgaccc gtctggcaaa ggagccaccg aatgagtcac acgcagtcga
     3361 agcatcgcgc acacctgcgc gcccccctgg ccctgctcgc tctcatcgcc ctcgctctcg
     3421 cgccggcggt ggcttcggcc ggggtcaagc ctccgcaggt caccctgaac tatgagaagt
     3481 acaccctccc gaacgggctc gaggtcatcc tctacgagga ccacaagctc ccgctcgtgg
     3541 cggtgaacac ctggtatcac gtcggccccg ccaacgagct gcccggccgg accggcttcg
     3601 cccacctgtt cgagcacatg atgttccagt cctccgggca tgtcggcgag gatcagatct
     3661 ggaagtatct cgagggcgcc ggcgcctcct tcatcaatgg gacgaccgac ttcgatcgca
     3721 ccaactacat ggaggacatg ccctccaacc agctcgagac cgcgatctgg ctcgagagcg
     3781 atcggatggg ctacctcctc gacacgctga ccgggccctc gcttgcgaac cagcaggacg
     3841 tcgtgcggaa cgagcgccgg cagagcatcg agaacgcccc ctaccagatc gtcgaggaag
     3901 agatgtggca catgctcttc ccgaaggggc atccctacta cgcctggatc atcggatccc
     3961 acgaggacgt ccaggcggcg aagctcgatg acgtgaagaa cttcttccgc agctactacg
     4021 cgcccaacaa cgcgagcctc gtgatcgcgg gcgacatcga caaggcgaag gtgaaggcgc
     4081 tggtcgagaa gtactacggc tcgatcccaa agggcgagcc tgtgccggcc atcaccgcca
     4141 cgacgccgcc gatcacggcc gagcgccgcg cgacggtcac cgacaagatc acgctgcccc
     4201 gtgtctacat ggcctggctc acggcgccga tcttgaagcc gggcgacgcc gaggcgggcg
     4261 tggccgcgga cattctgggc ggcggcaagg cgagccggct ctacaagagc ctcgtgtaca
     4321 cgaagcggat ctgccaggac gtctccgtcg tgcagcagtc gtacgcgctg agctccgtgt
     4381 tccagatctc ggccaccgtc aagccgggcc acacgccgga cgaggtggag aaagcgatca
     4441 acgaggagct cgccacgttc atccagaacg gcccgaccga cgccgaagtc acggccacgc
     4501 agaacgcgct ctactccaac atcgtgacga gcctggagaa cctgggcggc ttctccggca
     4561 ttgccgaccg gatgaactcg tacaaccagg ccgtcaagga tccgggttat ctcagcaagg
     4621 acctcgcgcg cttcgaggcg gtgacgcctg ccgcggtgaa gcaattcgcg gcgcagagcc
     4681 tccggaacga cgcgcgcgtc gtggtctacg cggtgccggg cgacaagaag ctcccgcccg
     4741 acccgccggc ccctccgcct ccgtcgaagg aggaggcgaa ggtcgagtcg aaggagccgt
     4801 ggcggaacac gccgccggcg gccggggccg agaaccaggc caagctcccg acgccgaaga
     4861 agatcgcgct tccgaacggg ctcacggtgt acgtggtcga gtcacaccgg ttcccgctcg
     4921 tgtcggcgaa cctggtgctt cgctcgggta gcgcggcgga tcccgccgac ctcccgggtc
     4981 tcgcgggcta cacggccgcc atgctcgacg aaggaacgac gaagcgtgac gccctcgcgc
     5041 tcgcggatca gacgcacgcg ctgggcgcct ccctcgtgac gggaagccag ccggacggct
     5101 cgagcgccgg catccggtcg ctcaagcaga acgccgcggc ggcgatcgac atcctctccg
     5161 acgtcacgct ccacccctcg ttccccgatc aggagatcga gcggatccgg aacgaccggc
     5221 tcacgacgct gatgcagcag cgcgatcagc cgtggccgac ggggatccgc gtcatgaacg
     5281 cctgcctgtt cggaccggcg catccctacg gccacacgca gatcggcacg caggcttcga
     5341 tcgagaagac gaagcgcgac gatctcgtca agttctacgg gtcgacgtac agccccaagg
     5401 acgccgccct gatcctggtc ggcgatctga ccgaggccga ggcgaagaag ctcgcgacgg
     5461 acgccttcgg cgcgtggaag ggtgcggcga cgccggctcc ggttcctccg acggggacga
     5521 tggccaactc gcgcatcgtg atcgtggaca agccgggctc ggtccagacg gtggtcctcg
     5581 ccgggcagct cggcgtcaag cgctcggacc ccgactacga gaagctcgac ctcatgaaca
     5641 ccgtcctcgg cgggctcttc tcgagccgca tcaacctgaa cctccgcgag gacaagggct
     5701 acagctacgg cgccttctcg ttcgtgggac a
//
LOCUS       DASTKC010000207         6244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1113541, whole genome shotgun sequence.
ACCESSION   DASTKC010000207 DASTKC010000000
VERSION     DASTKC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113541"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1778
                     /gene="pilM"
                     /locus_tag="VFR25_08620"
     CDS             171..1778
                     /gene="pilM"
                     /locus_tag="VFR25_08620"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET9941155.1"
                     /translation="MTGARAFTRRLGALLGETSRALIGVERGQGWARVFAPGGPPSAG
                     TSVRELVEALGLGGRSAAAMLEGSEVVVRRLTLPRMKGGDLRAALALECRNLIGYPIE
                     EAVIRHEVLGPSVQGAGVDVLVAVAPKRAVLESIRALESAGLVPRRLTIRAVALRSLL
                     RSARSLDRDEDVAYLDLGPRESHITIVRGDEIRFTRECGVGVASFIDALRSIVVPGQG
                     VRLLDAGEAETLLLSHGVLGGREEQPSSEIPASAVLVMLRPSLERLTRELWNSVDYVH
                     EQFHGQTVSKIALLGETARVPGLDSYLAGVLKMPVETVDSEQGGTLAWGGVAGLSHLD
                     PGAIDFLEPSDLGPLSRVAEAVQPRVAIAATALLLLSVSLPAEMSVQRERARITDLER
                     KLASVAPYADQVVRFREARSEEASSDALMASLRGPTPAWAEVLRDLSHRVGPDARLTS
                     VALVEPKPEDADAEAAATTPGAMTIRIEGLVKRERLRPEAALGGLMASLETSPYVDRV
                     ELVTCQAVGPAQSRFVLEARLSSGGTP"
     gene            1775..2347
                     /locus_tag="VFR25_08625"
     CDS             1775..2347
                     /locus_tag="VFR25_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941156.1"
                     /translation="MTRGVRHPVIRLALLILVLVVSTGAVRAVYVGPREREAAELKAR
                     YGSLAAKVGDLQRGIGDLAAWSNTHPAESVEAMRRRQAPPARELVPAFLKALEPIAAR
                     HGIVTETIDPVGPPAEETVPGLSGRPVELVRQSLRFRLRGAYRDIAEYVREVDGMSAF
                     VMARTVALRYDGASYPALQAEVRFDLYGAP"
     gene            2360..2614
                     /locus_tag="VFR25_08630"
     CDS             2360..2614
                     /locus_tag="VFR25_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941157.1"
                     /translation="MESVAIEVRKSVNPRTYVLLGVLALALAWAASEKGRPRHHAPSL
                     GATAAMTPTSAPLAEPTPVGWGRDPFDPRAVSQDVNGNGR"
     gene            2619..3020
                     /locus_tag="VFR25_08635"
     CDS             2619..3020
                     /locus_tag="VFR25_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9941158.1"
                     /translation="MTNQESPRGRVLVVDDEPDLVRVLEFGLKASGYTVEVASDGQEG
                     LKKAREIKPDVILLDLMLPKLDGYKVCRLLKFDDRFKHIPIIILSARTQEGDQTLALE
                     MGANRFVTKPYDFSEILGYIETLLKSPSVRP"
     gene            3140..4561
                     /locus_tag="VFR25_08640"
     CDS             3140..4561
                     /locus_tag="VFR25_08640"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HET9941159.1"
                     /translation="MKGQTERMPEQTGARTPAEPRIYDSILETVGRTPLVRLHAIAKD
                     CRAPIVAKLEAFNPGGSVKDRIAVAIMAEAEAKGLLKPGGTIVEATSGNTGTGLAMVA
                     AVKGYRSVLVMPDKVSKEKINLLKSFGAEVVITPTSVPPDSPESYYEVAKRIVRETPG
                     AYLANQYYNPTNPEAHYRTTGPEIWEQTAGKITHYVASLGTGGTISGTSRYLKQQNPK
                     IKTIGADPIGSILKAYFDTKKVIQPKPYKVEGIGEDFFPGTLDFSMIDEIFEVNDSQS
                     LNLARRLAREEGILSGGSAGTALYAALQVARRANPDDLVVVIIPDTGERYLSKVHSDE
                     WMRDNHLLDSNAVTVGEIIRAKGNHVPAIVTVSYDEPASRALALIREFNISQLPVLKD
                     GKVVGAVTEGALMQKVLDGTATPESRLEYLIEKSLPTVSLHERLPRVMKLLAERNAAL
                     AVDETGRPTGMLTRFDLIEYINA"
     gene            4561..5700
                     /locus_tag="VFR25_08645"
     CDS             4561..5700
                     /locus_tag="VFR25_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET9941160.1"
                     /translation="MGFSTDAIHAGQEPDPTTGAVTVPIYQTSTYVQQGLGKHKGFEY
                     ARTQNPTRIALEKNVAVLEKGAEGFAFGSGMAAITTITQLLKSGDHVIASNNMYGGTY
                     RLFERVVRPYGLDFTYVDTSCLNLVEAAFRPSTKMVFVETPTNPSMIVSDLRKLAELS
                     RSRGALLTVDNTFMTPYFQRPIELGAHLVVHSATKYLNGHSDMVGGVIVSNDAAASER
                     LRFLQNAAGAVPGPFDCWLVLRGIKTLAIRMDRHESNARAIAAWLSKNKKLQRVLYPG
                     LPDHPGRALHESQSSGYGGMIAFDVGSMEKGAAVLPRTKVFSLAESLGGVESLISHPA
                     TMTHASVPREEREKVGLTDGLIRISVGIEDLDDLIADLDHALAIL"
     gene            5935..6138
                     /locus_tag="VFR25_08650"
     CDS             5935..6138
                     /locus_tag="VFR25_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941161.1"
                     /translation="MKKFLTITLVFVFAIALVLAVGCAKKEQTSTEGTSTMDQQMEQP
                     APADTMATPMMTDTTAAGSTTTH"
BASE COUNT         1025 a         2091 c         2158 g          970 t
ORIGIN      
        1 gatcctggat tccagggaga cggaggcccg tccagccagg cgcgcctccg cgcgccggtc
       61 gggctcgcgg tcgccccgtc gggcgagtac tacatcggct gccgtctgaa cggccgcgtg
      121 cgcaaggtga ccggagtcct ctccgtcgtc gcgtggtcgg agtctcgtcc gtgaccggcg
      181 cccgggcctt cacgcgccgt ctcggcgcgc tcctcggcga gacgagccgg gctttgatcg
      241 gcgtcgagcg cggccagggc tgggcgcgcg tcttcgctcc cggcgggccc ccgagcgccg
      301 gcacctccgt gcgcgagctc gtggaggcgc tgggactcgg tggccgctcc gccgccgcga
      361 tgctcgaggg aagcgaagtc gtcgtgcggc gtctgaccct tccgcggatg aaggggggcg
      421 acctcagggc cgcgctcgcg ctcgagtgcc gcaacctgat cggctatccg atcgaggagg
      481 cggtgatccg ccacgaagtg ctgggccctt cggttcaggg ggcaggcgtg gacgtgctcg
      541 tcgccgtcgc gcccaagcgc gccgtcttgg agtcgatccg cgcgctcgag tcggcggggc
      601 tcgtcccccg gcggctcacc atccgcgcgg tggcgcttcg ctcgctcctc cgctcggcgc
      661 ggtcgctcga cagggacgag gacgtggcgt atctggacct cggtccgcgc gagagccaca
      721 tcacgatcgt ccgcggggac gagatccggt tcacccgcga atgcggcgtc ggcgtcgctt
      781 cgttcatcga cgccctccga tccatcgtgg tgcccggcca gggcgtgcgg cttctcgacg
      841 cgggtgaagc cgagaccctg ctcctctcgc atggggtcct gggcggacgc gaggagcagc
      901 cctcctccga gatccccgcc tccgccgtgc tggtcatgct ccgcccgagc ctcgagcgcc
      961 tcacgcggga gctctggaat tcggtcgact acgtccacga gcagttccac gggcagacgg
     1021 tctcgaagat cgcgctcctc ggcgagacgg cgcgcgtgcc cggcctggac tcctatctgg
     1081 cgggcgtcct caagatgccg gtcgaaacgg tcgattctga acagggaggg acgctcgcgt
     1141 gggggggcgt ggccggcttg agccacctcg acccgggcgc gatcgacttc ctcgagccgt
     1201 ccgacctggg gccgctctcg cgggtggcgg aggcggtgca gccgagggtc gcgatcgccg
     1261 ccaccgcgct gctcctcctc tcggtgtcgc tgccggccga gatgagcgtc cagcgggagc
     1321 gcgcgcggat cacggatctc gagcggaagc tcgcgtccgt ggcaccgtac gcggatcagg
     1381 tcgtccggtt ccgcgaggcg aggagcgagg aagcgtcctc ggacgctttg atggcgtcgc
     1441 tccgcgggcc gacgcccgcg tgggcggagg tgctccgcga tctgagccac cgcgtgggcc
     1501 cggacgcgcg gctcacttcc gtcgctctgg tcgagccgaa gcccgaggac gcggatgccg
     1561 aggccgccgc gacgacgccc ggcgcgatga cgatccggat cgaggggctc gtgaagcgcg
     1621 agcggctccg tccggaggca gcgctcgggg gtctcatggc gtcgctcgag acctcgccgt
     1681 acgtggaccg ggtcgagctc gtgacctgcc aggcggtggg accggcgcag agtcgcttcg
     1741 tgctggaagc caggctgtcg agcggaggga cgccatgacc aggggcgtgc ggcatccggt
     1801 gatccggctc gcgctcctga tcctcgtgct cgtcgtgtcg accggggcgg tgcgtgcggt
     1861 gtacgtcggg ccgcgcgagc gggaagcggc ggagctcaag gctcgctacg gatcgctcgc
     1921 ggcgaaggtg ggcgatctcc agagggggat cggcgatctc gctgcctggt cgaacacgca
     1981 cccggcggag agcgtcgaag cgatgcgccg acggcaggca ccgccggcgc gcgagctcgt
     2041 gcccgcgttc ttgaaggcgc tcgagccgat cgcggcgagg cacgggatcg tgacggagac
     2101 catcgatccc gtgggtccgc cggcggagga gacggtgccg ggtctctcgg gcaggccggt
     2161 tgagctcgtg cggcagagcc ttcggttccg gctccggggc gcgtatcgcg acatcgcgga
     2221 gtacgtgcgc gaagtggatg ggatgagcgc gttcgtgatg gcgagaacgg tcgcactccg
     2281 gtatgacggc gcgagctatc ccgcgctcca ggccgaggtc cggttcgacc tctacggggc
     2341 accatgagag gcgggttgaa tggaatcggt tgcgatcgag gtgcggaagt cggtgaatcc
     2401 gaggacgtac gtgttgctcg gcgtgctggc cttggcgctc gcctgggccg cctcggagaa
     2461 gggaagaccg cggcaccacg cgccgagtct cggagcgacg gccgcgatga cgccgacctc
     2521 ggccccgctc gcggagccga cgccagtcgg gtgggggagg gatccgttcg atccccgcgc
     2581 tgtgtcccag gacgtgaacg gaaacgggag gtgacgtcgt gacgaatcag gaatccccgc
     2641 gaggacgcgt cctcgtcgtc gacgacgagc cggatctcgt gcgcgtgctc gaattcgggc
     2701 tcaaggcttc gggctacacg gtcgaagtgg cgtcggacgg acaggaaggc ttgaagaaag
     2761 cgcgcgagat caagccggac gtgatcctgc tcgacctcat gctacccaag ctggacggtt
     2821 acaaggtgtg ccgtctgctc aagttcgacg accggttcaa gcacatcccg atcatcatcc
     2881 tgtcggcgcg cacgcaggag ggggaccaga ccctcgccct cgagatgggg gccaatcgct
     2941 tcgtcacgaa gccctacgac ttcagcgaga tcctcgggta catcgagacg cttctcaagt
     3001 ctccgtccgt gcgcccctga cccactccgc cgcgctcgtc cgggcggcgg ccgcgaaatc
     3061 cgcgcttgac cccgcgcggc cggaaccgta gcctttttgc gcggttccgt cgcgtttacc
     3121 ttccgccgaa gcgaacctca tgaaaggcca gaccgaacgc atgccggagc agacgggcgc
     3181 tcgcacgccc gccgagccac gcatttacga cagcattctc gagactgtcg gccggacgcc
     3241 cctcgtgcgg ctccacgcga tcgccaagga ctgccgcgct ccgatcgtgg ccaagctcga
     3301 agccttcaat ccgggcggct ccgtgaagga ccggatcgcg gtcgcgatca tggccgaggc
     3361 cgaggcgaag ggactcctga agccgggggg cacgatcgtc gaggccacgt ccgggaacac
     3421 cgggaccggt ctcgccatgg tggccgcggt gaagggctac cggtccgtgc tcgtcatgcc
     3481 ggacaaggtg agcaaggaga agatcaacct cctcaagtcc ttcggcgccg aggtcgtgat
     3541 caccccgacg tcggttccac ccgattcgcc cgagtcctac tatgaggtcg cgaagcggat
     3601 cgtgcgcgag accccgggcg cgtatctcgc gaaccagtac tacaacccga cgaatccgga
     3661 agcccactac cgcacgaccg ggccggagat ctgggagcag acggcgggga agatcacgca
     3721 ctacgtggcc agcctcggca cggggggcac gatcagcggc acctcgcgct acttgaagca
     3781 gcagaacccc aagatcaaga cgatcggcgc cgatcccatc ggctcgatcc tcaaggcata
     3841 cttcgatacg aagaaggtga tccagcccaa gccctacaag gtggagggaa tcggcgagga
     3901 cttcttcccc ggcacgctcg acttctcgat gatcgacgag atcttcgagg tcaacgacag
     3961 ccagtcgctc aacctggccc gccgcctcgc gcgcgaggag gggatcctct ccggtggatc
     4021 ggccggcacc gcgctctacg cggcgctcca ggtggcgcgc cgcgccaatc cggacgacct
     4081 cgtcgtcgtc atcatccccg acaccgggga gcggtatctc agcaaggtgc actcggacga
     4141 gtggatgcgg gacaaccacc tgctcgactc gaacgcggtc acggtgggcg agatcatccg
     4201 ggccaaggga aatcatgtgc cggcgatcgt gacggtgagc tacgacgagc ccgcctcgcg
     4261 cgcgctcgcc ctgatccgcg agttcaacat ctcgcagctc ccggtgctca aggacggcaa
     4321 ggtggtcggc gcggtgacgg agggggccct gatgcagaag gtcctggacg gcaccgcgac
     4381 tccggagtcc cgcctcgagt acctgatcga gaagtcgctc ccgaccgtgt cgctccatga
     4441 gcggctgccg cgcgtgatga agctcctcgc ggagcgaaac gccgcgctcg cggtggacga
     4501 aacgggccgg ccgaccggca tgctcacgcg cttcgacctg atcgaataca tcaacgcctg
     4561 atgggcttct cgaccgacgc catccacgcc gggcaggagc ccgatcccac gacaggggcc
     4621 gtcacggtcc ccatctacca gacctcgacc tacgtccagc aggggctcgg gaagcacaag
     4681 ggcttcgaat acgcccgcac ccagaatccg acgcggatcg cgctcgagaa gaacgtggcc
     4741 gtgctcgaga agggggcgga gggcttcgcg ttcgggtcgg ggatggcggc catcacgacg
     4801 atcacccagc tcttgaagtc gggggatcac gtgattgcct ccaacaacat gtacggcggc
     4861 acctaccggc tcttcgagcg ggtggtgcgt ccctacggcc tcgacttcac gtacgtcgac
     4921 acctcgtgcc tgaacctggt cgaggccgcg ttccggccct ccaccaagat ggtcttcgtg
     4981 gagacgccga cgaacccctc gatgatcgtc tccgatctcc ggaagctcgc ggagctatcg
     5041 cgctcccgcg gggcgctcct cacggtcgac aacacgttca tgacccccta cttccagagg
     5101 cccatcgagc tcggcgccca cctcgtcgtg cacagcgcga ccaagtacct caacggacac
     5161 agcgacatgg tgggcggggt catcgtctcg aacgacgccg ccgcgagcga gcggctccgg
     5221 ttcctccaga acgcggccgg cgcggtgcct ggcccgttcg actgctggct cgtgctgcgc
     5281 ggcatcaaga ccctcgcgat ccgcatggac cgccacgagt cgaacgcgcg cgcgatcgcc
     5341 gcgtggctgt ccaagaacaa gaagctccag cgcgtcctct accccggtct gcccgaccat
     5401 ccgggacgcg cgctccacga gagccagtcg agcggctacg gcgggatgat cgcgttcgac
     5461 gtcggatcga tggagaaggg ggccgcggtc cttcccagga ccaaggtctt ttccctggcc
     5521 gagagcctgg gcggggtcga gagcctgatc agccaccccg ccaccatgac ccacgcctcc
     5581 gtgccccgcg aggagaggga aaaggtcggg ctaacagacg gtttgataag aatttccgtc
     5641 ggcatcgagg acctcgacga cctgatcgcg gacctcgacc acgccctcgc gatcctctga
     5701 ccccgcaaag agggacttga caggatttcg ggggctccgt actgtccgcg ccgttcttgc
     5761 cgcggggggt cccggcggcg aaacgggtcg aaatcgtacc cgctgctgtt cgaaaattgc
     5821 acctgttcgc cccggcaccg ctgtacccag tggtgttttg ctttatgggg tggaaccggt
     5881 tgcaccaccg caatcgatag tcgatgtctt caatcgaagg gaggtgattg cggaatgaag
     5941 aagtttctga ccatcacgct ggtgttcgtg ttcgccatcg cgctggttct cgcggtcggc
     6001 tgtgcgaaga aggagcagac ctcgaccgag ggcacttcga ccatggatca gcagatggag
     6061 cagccggccc ctgccgacac gatggcgacg cccatgatga ccgacaccac ggcggcgggg
     6121 tccacgacga cccactaatc gggttgcgtc tcgatccagg ttttccgcta ccgacgacga
     6181 aacgagctta agaggcagca cgcataaagt agcgagtgat acgggaggga agcgggcgca
     6241 cggc
//
LOCUS       DASTKC010000208         9631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1823204, whole genome shotgun sequence.
ACCESSION   DASTKC010000208 DASTKC010000000
VERSION     DASTKC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823204"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFR25_08655"
     CDS             complement(<1..198)
                     /locus_tag="VFR25_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941162.1"
                     /translation="MATDTRTTTPSRTPLLVTESLPGGLALLGGLNHVARALVPFDPG
                     GTSLAQVVLGVKRILRSDGTAI"
     gene            complement(370..1107)
                     /locus_tag="VFR25_08660"
     CDS             complement(370..1107)
                     /locus_tag="VFR25_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9941163.1"
                     /translation="MFEAQPSQVSLIAAFLAGLVSFVSPCVLPLVPSYVTFITGLSFD
                     ELTGEQGPRVRRLTLIHSLAFIFGFSLVFIALGATATATGQFLREHQDTLRIVGGVLI
                     VFFGIYLTGLIKIPALSRERKVHLTGKPLGVLGSVLVGITFAAGWTPCIGPILASILL
                     YASTAQTVGTGILLLSVYSLGLGVPFLIASLGMNTFLSASKGIRKQLHTIEVVSGVIL
                     IVFGLALVTNVFGYFVAFLSRFLPAVG"
     gene            complement(1119..1544)
                     /locus_tag="VFR25_08665"
     CDS             complement(1119..1544)
                     /locus_tag="VFR25_08665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9941164.1"
                     /translation="MGTTAPAFALPDLEGREVKDTDLRGKVVILDFWATWCGPCRLEV
                     PHFVRLQSKYREQGLQIVGLSMDAGGARDVRPFVDEHDINYTMLIATDKTAQDFGGIL
                     GIPTTFVLDRKGTIVKKFIGYTDPAVFEEVIAPLLAQAS"
     gene            complement(1657..2988)
                     /locus_tag="VFR25_08670"
     CDS             complement(1657..2988)
                     /locus_tag="VFR25_08670"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET9941165.1"
                     /translation="MESLKTAKDVVKFCEERGVEMIHLWFVDILGQLKAVAITLRELG
                     TALEEGVGVDGSSVEGFARIYESDLVAKPDVSTFQMLPWKVNGENVGRLICDIHNPDG
                     SPYMGDTRYVMKRLVKKLAKDGYTAFLGPELEYFYFKGLKDRTLLDTAGYFDQVPDDI
                     GTELRSRTVEALQAMEISVEASHHEVAHSQHEIDLRYSEALKMADQTITYRYVVKEIA
                     RQGGYYATFMPKPVHGQNGSGMHVHQSLFKAGKNVFFDAKDKHHLSKAGRSYLAGALA
                     HMREITSVTNQWVNSYKRLVPGFEAPVYIAWGQRNRSALVRVPMYKPGKEKATRIELR
                     CPDPACNPYLAFSLCIAAGLKGMESALTLRASVEDDIYEMNAAERKEHDIESLPGSLS
                     EAVELTAKSRLVKDTLGEHVFTKFLENKRIEWDNYRTWVTDYELNEYYPIL"
     gene            complement(3044..3436)
                     /locus_tag="VFR25_08675"
     CDS             complement(3044..3436)
                     /locus_tag="VFR25_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9941166.1"
                     /translation="MDVTDDRNRVDVEQLLELYRTTWWAHDRSRDLVRRALDHSHPVL
                     TVWDGPTLIGFTRVISDLTFRATIWDVIVRPSHQGRGIGTQLMHAVLDHPDLKTVTSF
                     FLLTRDKHSFYERLGFRHEPDKSMILRR"
     gene            3602..5350
                     /locus_tag="VFR25_08680"
     CDS             3602..5350
                     /locus_tag="VFR25_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941167.1"
                     /translation="MLTRTSLGRFFRKKAPTPSPPELLEALDARRAAEESLRRSRENF
                     YVIVQGVRDYAILMLDPTGVIITWNEGAQRIKGYAPDEILGKHFSVFYPEEAVRSKWP
                     ETELENAAKTGRFEDEGWRVRKDGTRFWANVVITTLYHPDGTVRGFLKITRDLTDRMR
                     SEEGLRHARTELEMRVNERTTELGEANRALKTEIAERHLLEQELRKRVAGMAEEDRRK
                     NEFLATLAHELRNPLAPIQYAYELMNLAGNNEALRSEAQGILHRQVQHMVRLIDDLLD
                     LSRITRNKLELRRDRVDLATVLEDSVETVRPALEARGHDFRVRLAPGPVLVHADRTRL
                     AQVFSNLLSNAAKFTERGGRIRLTSERQDDHVVVHVRDDGIGIAPAMLPRIFDMFVQA
                     DRSLERTQSGLGIGLTLVKRVVEMHGGTVVAESEGPGRGSDFSVSLPLEPAAADRKRE
                     AGPSDEDMTSTTSVRVLVADDNEDAVRSLAMMIRAMGHEVHTARDGAEVLEVAATIVP
                     TLILMDIGMPRMNGYDAARRIREQPWGRTITLVALTGWGLEEDRRLASEAGFDRHVVK
                     PVEAATLREILASVIA"
     gene            5347..5769
                     /locus_tag="VFR25_08685"
     CDS             5347..5769
                     /locus_tag="VFR25_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9941168.1"
                     /translation="MKHLRKTLTFNIPARMDLVNITPQVEAALEESGIQEGLCLVNAM
                     HITASVFINDDESGLHADYKRWLEQLAPFDASPERYRHNRTGEDNADAHLKRQVMGRE
                     VVVAVTKGKLDFGPWEQIFYGEFDGRRAKRVLIKIIGE"
     gene            complement(5801..6478)
                     /locus_tag="VFR25_08690"
     CDS             complement(5801..6478)
                     /locus_tag="VFR25_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9941169.1"
                     /translation="MSQEKPHPEGPGKRGIGSGETPAAASTRLQAIRIALSHDLTRAG
                     RVLERRGMVVATEGNLSARLTPERFLISRRGRRKGELKSRDYVEVSVVDSPETPVPLN
                     ASSEYRVHRVAYRARRDAEAVLHAHPVSLTAYAVRGVLPDFARFDEGRIFVGPIVLVP
                     HFPSGSDALAEAVELALVEPTRPSLVLMQNHGALSVGQSVDEALSRLEVAEHLAATLI
                     AAGRVPD"
     gene            complement(6475..8139)
                     /locus_tag="VFR25_08695"
     CDS             complement(6475..8139)
                     /locus_tag="VFR25_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9941170.1"
                     /translation="MRLPPAMSRVVTADEMVAIDRAAIARGVPSLTLMERAGKEAARR
                     IVGWWRSADGGGRARSAAPAGRTRTASRAAQAAGPVLVLCGRGNNGGDGFVAARHLKA
                     AGFPVRTLVASSEDSLSADARVQYESCSRARVPVTFLPDPRAWGPGSEAADAASQAAF
                     LVDALLGTGSKGAPRGAVAAAIELAVASGKPIASIDIPSGVDATTGHVEIPAIRADVT
                     VTLALPKVGLRLEPGREHAGLVEVADIGIPSDLVDSGRPTVAVATAGWARSLLPRRAP
                     DAHKGSVGRVLVVGGSHGLMGAVAMAGESVLRSGAGYSVAAVPESGVAALESRVAEVV
                     KRPCPETASRSLSQDALDAILAEAMRADVVAIGPGLSRDLETSALVRELVLRIDAPLV
                     LDADGLNAFEGHGLKRTHGPLIVTPHYGEAARLAGVSISDVAKDPIGWARSFASVSGA
                     IVCLKCVPMVTVAPGEPAILNATGNPGMATAGAGDVLTGTIAGLMAQGMDPPEAAAVA
                     CFVHGLAGDIAARRQGRRGMIAGDIRDALASALVALESGALDEGAG"
     gene            complement(8141..8500)
                     /gene="acpS"
                     /locus_tag="VFR25_08700"
     CDS             complement(8141..8500)
                     /gene="acpS"
                     /locus_tag="VFR25_08700"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET9941171.1"
                     /translation="MVSVGVDIVEVRRIAEAILRWGDKFVERIYTPGEIDYCLRRARQ
                     AESFAVRFAAKEAFAKALKVGKASIWREVEVVRSEGPRPTFQLHGNARLLVGHRKVDL
                     SLSHAQTHAVAVVLIED"
     gene            complement(8508..9227)
                     /locus_tag="VFR25_08705"
     CDS             complement(8508..9227)
                     /locus_tag="VFR25_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9941172.1"
                     /translation="MIRAIIFDLDNTLTDFMKMKRAAIDAAVDGMRDAGLALPRDEVA
                     SRIYQVYDREGIEYQQVFDIFLKEALGRVDYKILSSGIVAYRRARDSYLVLYPHVNLT
                     LLELLKRGLKLAVVSDAPRLQAYMRLAHLQLHHLFDTVVAFEDTGERKPSPKPFQRAI
                     EMLGIQPQEAIMVGDWPERDVVGAAQVGLRTAFARYGDTFGTVNSGADYELNDVYELV
                     AIVDELNGTLLSAVDGLNGRD"
BASE COUNT         1615 a         3264 c         3290 g         1462 t
ORIGIN      
        1 gatcgccgta ccgtcggagc gaaggatccg cttcacgccg agcacgacct gcgcgagcga
       61 cgtgccgccc ggatcgaacg gcacgagggc gcgcgcgacg tgattgagtc cgcccaggag
      121 cgcgagccct cccggaagcg attcggtgac gagaagtgga gtccggcttg gagtggttgt
      181 ccgcgtgtcg gtggccatgc gctcctccct ggattacggt ccccccgtgt atcgggccgg
      241 ggtgcgccaa tgtttagggg tcgaacggcc gcaagacgct agaattccag ggggttgcaa
      301 gctgcccggc gcggggcgcg ggcgtgcgtc tcgcaatggc ggatcgagcc attgcggggt
      361 cgtgcggctc tatccgaccg cggggaggaa cctcgagagg aaggcgacga agtagccgaa
      421 cacgttcgtc acgagcgcca gaccgaacac gatcaggatc acgccgctca cgacttcgat
      481 cgtgtggagc tgcttgcgaa tgcccttcga cgcggacagg aaggtgttca tgccgagaga
      541 cgcgatgagg aacggcacgc ccagcccgag cgagtagacg ctcagcagaa ggatgcccgt
      601 ccccaccgtc tgggccgtgc tcgcgtagag gaggatcgag gcgaggatcg gaccgatgca
      661 cggcgtccat cccgcggcga acgtgatccc gacgagcacc gagccgagga cgcccagcgg
      721 ctttccggtg agatgcacct tccgctcgcg cgagagcgcg gggatcttga tgagcccggt
      781 gagatagatc ccgaagaaca cgatcaagac gcccccgacg atccggagcg tgtcctggtg
      841 ctcgcggagg aactgccccg tcgcggtggc cgtcgcgccg agcgcgatga agaccaggga
      901 gaagccgaag atgaaggcga gcgaatggat cagcgtgagc cgccggacgc gcggcccctg
      961 ctcccccgtc agctcgtcga agctgagccc ggtgatgaac gtgacgtagg acggcacgag
     1021 cgggagcacg cacggcgaga cgaacgacac gagtccggcc aggaacgccg cgatcagcga
     1081 gacctggctc ggctgcgcct cgaacattcc ggtgcccatc agctcgcctg ggcgaggagc
     1141 ggcgcgatca cttcctcgaa caccgcggga tcggtgtacc cgatgaactt cttcacgatc
     1201 gtgcccttgc ggtcgagcac gaacgtggtc gggatgccga ggatgccgcc gaagtcctgc
     1261 gcagtcttgt cggtggcgat gagcatcgtg tagttgatgt cgtgctcgtc gacgaacgga
     1321 cgcacgtccc gcgcgcctcc ggcgtccatc gagagcccca cgatctggag cccctgttcg
     1381 cggtacttgg attgcagccg gacgaagtgg ggcacttcca gccggcacgg gccgcaccac
     1441 gtggcccaga aatcgaggat cacgaccttg ccgcggaggt ccgtgtcctt cacctcgcgc
     1501 ccctcgagat ccgggagcgc gaaggcgggc gccgtggtgc ccacggcacc ccctcccttg
     1561 gccgtcgtcg tctcgggcgc cttgccgcaa ccgcccgaca aagcaagcgc cagcaccagc
     1621 gatagaacgc cggcgccgag cgccaatccg aagagcttag agaatcgggt agtactcatt
     1681 gagctcgtag tcggtgaccc acgtccggta gttgtcccac tcgatgcgct tgttctcgag
     1741 gaacttcgtg aagacgtgct cgcccagcgt gtccttcacc agccgcgact tcgcggtcag
     1801 ctcgacggcc tcgctcaagc tccccgggag gctctcgatg tcgtgctcct tccgctcggc
     1861 cgcgttcatc tcgtagatgt cgtcctcgac cgacgcgcgg agcgtgaggg ccgactccat
     1921 gcccttcaag cccgccgcaa tgcaaagcga gaacgcgaga taaggattgc acgccggatc
     1981 cgggcagcgg agctcgatac gggtcgcctt ctccttgccc ggcttgtaca tcggtacccg
     2041 cacgagcgcc gaccggttcc tttgacccca cgcgatgtag accggggcct cgaagccggg
     2101 cacgagccgc ttgtacgagt tgacccactg gttcgtgacc gaggtgatct cgcgcatgtg
     2161 ggcgagggcg cccgccaggt acgagcggcc ggccttggac aggtggtgct tgtccttggc
     2221 gtcgaagaag acgttcttcc cggccttgaa gagcgactgg tggacgtgca tgccgcttcc
     2281 gttctgaccg tggaccggct tcggcatgaa cgtggcgtag tagccgccct ggcgcgcgat
     2341 ctccttcacg acgtagcggt aggtgatcgt ctggtcggcc atcttgagcg cttcgctgta
     2401 gcgcaggtcg atctcgtgct ggctgtgcgc cacttcgtgg tgcgacgcct cgaccgagat
     2461 ctccatcgcc tggagcgctt cgaccgtgcg ggagcggagc tcggtgccga tgtcgtccgg
     2521 cacctggtcg aaatagccgg cggtatcgag gagcgtgcga tctttgagtc ctttgaagta
     2581 gaagtattcg agctccggtc cgaggaaggc cgtgtagccg tccttggcga gcttcttcac
     2641 gagccgcttc atgacgtagc gggtgtcccc catgtacggg gagccgtcgg gattgtggat
     2701 gtcgcagatg agccggccga cattctcgcc gttgaccttc caggggagca tctggaaggt
     2761 gctgacgtcc ggcttcgcga ccagatcgct ctcgtagatt cgcgcaaagc cctcgaccga
     2821 cgagccgtcc acgcccacgc cttcctcgag cgcggtgccg agctcgcgca gcgtgatggc
     2881 gacggccttg agctggccca ggatgtccac gaaccagagg tggatcatct ccacgccgcg
     2941 ctcttcgcag aacttcacga catccttggc cgtcttcaaa ctctccattc cgctcctctc
     3001 cttcttgatg gaggtgaaag gcggggtccc gccgggtgcc ggctcatcgc cgaaggatca
     3061 tcgacttgtc gggttcgtga cgaaaaccca gacgctcgta gaacgaatgc ttgtcccggg
     3121 tcaaaaggaa gaaggatgtg accgtcttga gatccggatg gtcgagcacc gcgtgcatga
     3181 gctgggtgcc gatgccccgg ccctgatgcg acggccgcac gatcacgtcc cagatggtgg
     3241 cacggaacgt gagatccgag atgacgcggg tgaagccgat cagcgtgggt ccgtcccaca
     3301 ccgtgagcac cggatgtgag tgatcgaggg cacgacggac cagatcgcgc gagcgatcgt
     3361 gagcccacca ggtggtccgg tagagctcca gcaactgctc cacatcgacg cggtttcggt
     3421 cgtccgtgac gtccacgatc ttgtctggtt gggagtgtgt ggtgcggcaa gcccggacaa
     3481 taccggggag agggcgttcc gtcaataccc ccctgatcgg acttagctcc atctcgcgca
     3541 acgggttgca gcttgcgggg gttgcgcccc gaaccgacag gtgcgtagac tcaaaggcag
     3601 catgttaacc cgcacttcac tcggacgctt ctttcgcaag aaggccccca cgccctcgcc
     3661 tcccgagctc ctcgaagccc tcgacgcccg tcgagccgcg gaggagtcgc tccggcgctc
     3721 gcgcgagaac ttctacgtca tcgtgcaagg cgtccgcgac tacgcgatcc tcatgctcga
     3781 ccccacgggc gtgatcatca cgtggaacga gggagcgcag cgaatcaagg gctacgcgcc
     3841 cgacgagatc ctcggaaagc acttttccgt cttctatccc gaggaggcgg ttcgctcgaa
     3901 gtggcccgag accgagctcg agaacgccgc gaagaccggg cgcttcgagg acgagggatg
     3961 gcgcgtgcgc aaggacggga cgcgcttctg ggccaacgtg gtgatcacca cgctctacca
     4021 ccccgacgga acggtgcgcg gattcctcaa gatcacgcgc gacttgaccg accggatgcg
     4081 ctccgaggaa ggactacgcc acgcgcggac ggagctcgag atgcgcgtga acgagcgcac
     4141 caccgagctg ggcgaggcga atcgcgcgct caagacggag atcgccgagc gccatctcct
     4201 cgagcaggag ctaaggaagc gggtcgccgg gatggccgag gaggaccgac gcaagaacga
     4261 gttcctcgcc accctcgcgc acgagctgcg gaatccgctc gctccgatcc agtatgccta
     4321 cgagctcatg aacctcgccg gcaacaacga ggccctgcgc agcgaagccc aggggatcct
     4381 ccaccgccag gtgcagcaca tggtgcggct gatcgacgac ctcctcgatc tctcgcgcat
     4441 cacccggaac aagctcgagc ttcggcgcga ccgggtcgat ctggcgacgg tcctcgagga
     4501 ctccgtcgag acggtgcggc cggccctcga agcgcgcggt cacgacttta gagtccggct
     4561 cgcgccgggc cctgttctcg tccacgccga tcgcacgcgg ctcgcgcagg tcttctcgaa
     4621 ccttctcagc aatgcggcca agttcacgga gcgcggcggc cggatccggc tcacgtccga
     4681 gcggcaggac gatcacgtcg tggtccacgt ccgggacgac gggatcggga tcgccccggc
     4741 gatgctgccc cgcatcttcg acatgttcgt gcaagccgac cggtcgctcg agcggaccca
     4801 gagcgggctc gggattggcc tcacgctggt gaagcgcgtg gtcgagatgc acggtgggac
     4861 ggttgtggcg gagagcgagg gcccgggacg cggaagcgac ttctccgtta gcctccccct
     4921 ggagccggcc gcagcggacc gcaagcgaga ggcaggaccg agtgacgagg acatgacttc
     4981 aaccacgagc gtccgggtgc tcgtcgccga cgacaacgag gacgcggtcc ggagccttgc
     5041 gatgatgatc cgcgcgatgg ggcacgaggt gcacaccgcg cgagacggcg ccgaagtcct
     5101 ggaagtggcg gccacgatcg tgccgactct catcctcatg gacatcggca tgccgcgcat
     5161 gaacggctac gacgccgcgc ggcgcatccg cgagcagccg tgggggcgca ccatcacgct
     5221 cgtcgcgctc acgggatggg ggctcgagga agacaggcgc ctcgcctccg aggcgggctt
     5281 cgaccgccac gtcgtgaagc cggtcgaggc ggccacgctc cgcgagatcc tggccagcgt
     5341 catcgcgtga agcacctccg caagacgctc accttcaaca ttccggcgcg gatggatctc
     5401 gtcaacatca cgccccaggt cgaggccgcg ctcgaggaga gcgggatcca ggaagggctc
     5461 tgcctggtga atgcgatgca catcacggcg agcgtcttca tcaacgacga cgagtccggt
     5521 ctccacgccg actacaagcg ctggctcgag cagctcgccc ccttcgacgc gagcccggag
     5581 cgctaccgcc acaaccggac cggcgaggac aacgccgacg cgcacctgaa gcgccaggtc
     5641 atggggcgcg aggtggtggt ggccgtgacg aaggggaagc tcgacttcgg cccgtgggag
     5701 cagatcttct acggggagtt cgacgggcgc cgcgcgaagc gcgtgctgat caagatcatc
     5761 ggggagtagc gcggccgctg cggcgcggcc cgacggccgt ctagtccggg acccgccccg
     5821 ccgcgatcaa ggtcgcggcc agatgctccg ccacctcgag ccgcgagagc gcctcgtcca
     5881 cgctctgccc caccgacaag gcgccgtggt tctgcatcag gacgaggctc ggccgcgtgg
     5941 gctcgaccag cgccagctcc accgcctccg ccagcgcgtc gctcccggac ggaaagtgcg
     6001 gcaccagcac gatcggcccg acgaagatcc gcccctcgtc gaagcgggcg aaatccggaa
     6061 gcacaccccg aaccgcgtac gcggtgagcg agaccgggtg cgcatggagc accgcctcgg
     6121 catcgcggcg cgcgcggtac gcgacccgat gcacccggta ctcgctcgac gcgttgagcg
     6181 gcaccggagt ctcgggcgag tccaccaccg agacctcgac gtagtcgcgg ctcttcagct
     6241 cgcccttgcg ccggccgcgg cgcgagatca ggaaccgctc gggcgtgagg cgcgcgctca
     6301 ggtttccctc ggtggccacg accatgcccc gccgctcgag cacgcggccc gcgcgcgtca
     6361 ggtcgtggct cagtgcgatg cggatcgcct ggagccgcgt cgacgcggcc gcgggcgtct
     6421 cgccggagcc gatgccccgc ttccccggtc cctcggggtg cggcttctcc tggctcaccc
     6481 ggccccctcg tcgagcgccc cgctctccag cgccacgagc gccgacgcga gcgcgtctcg
     6541 gatgtccccg gcgatcatgc cgcgccgtcc ctgccgccgc gccgcgatgt ccccggccag
     6601 gccgtgcacg aagcaggcga ccgcagccgc ctcgggcggg tccatgccct gcgccatgag
     6661 ccccgcgatg gtgcccgtga gcacatcccc ggcgcccgca gtcgccatcc cagggttccc
     6721 cgtcgcgttc aggatcgcgg gctcgccggg cgcgacggtc accatcggca cacacttcaa
     6781 gcagacgatg gcgccgctta cggacgcgaa cgaccgcgcc catccgatcg gatccttggc
     6841 gacgtcggag atggagacgc cggcgagccg ggccgcctcg ccgtagtgcg gcgtgacgat
     6901 gagcgggccg tgcgtgcgct tcagtccatg cccttcgaat gcgttcaagc cgtccgcgtc
     6961 gagaacaaga ggcgcgtcga tgcgcaggac gagctcccgc accagggccg aggtctccag
     7021 gtcgcgcgag agcccgggcc cgatcgcgac cacgtcggcc cgcatggcct cggcgaggat
     7081 cgcgtcgagc gcgtcctggc tcagggagcg cgacgccgtc tcggggcagg ggcgcttcac
     7141 gacctcggcc acgcgcgatt cgagcgcggc gaccccgctt tccggcaccg ccgcgacgga
     7201 atagccggct ccggagcgaa ggacggactc gcccgccatc gcgaccgcgc ccatcaagcc
     7261 gtggctcccg ccgacaacga gcacgcgccc cacggagccc ttgtgcgcgt cgggggcgcg
     7321 gcgcgggagg agcgaccgcg cccagcctgc cgtcgcgacc gcaaccgtgg gacggcccga
     7381 gtccacgaga tcggacggaa ttccaatgtc ggcgacctcg accagccccg cgtgctcgcg
     7441 cccaggctcg aggcggagcc cgaccttggg cagcgcgagg gtgaccgtga cgtcggcgcg
     7501 aatcgcgggg atctcgacgt gccccgtcgt ggcgtcgacg ccggacggaa tgtcgatcga
     7561 ggcgatgggc tttccggacg cgaccgcgag ctcgatcgcg gcggcgaccg caccgcgcgg
     7621 cgcccccttg ctgccggtgc cgaggagcgc gtccacgagg aacgcagcct ggctcgcggc
     7681 atccgcggcc tcgctccccg ggccccacgc gcgggggtcg gggaggaagg tcaccgggac
     7741 gcgggctcgc gagcagcttt cgtactgaac gcgcgcgtcc gccgagagcg agtcctcgct
     7801 cgaggcgacg agcgtgcgca ccgggaagcc cgccgctttc aagtggcgcg cggcgacaaa
     7861 gccatccccg ccgttgttcc cgcggccgca gagaacgagc acggggccgg ccgcttgggc
     7921 ggcgcgcgaa gcggtccggg tgcgtcccgc cggcgctgcg ctccgcgctc gtccgccgcc
     7981 gtcggcggac cgccaccagc cgacaatgcg ccgcgcggcc tccttccccg cccgctccat
     8041 gagcgtgagc gaggggacgc cgcgcgcgat cgcggcgcgg tcgatcgcga ccatctcgtc
     8101 ggccgtgacg acccgcgaca tggccggggg caggcgcacc ctagtcctcg atgagcacga
     8161 ccgcgaccgc gtgggtctgc gcgtgggaga gggagaggtc gaccttgcgg tggcctacga
     8221 gcaagcgggc gttgccgtgg agctggaacg tggggcgggg tccttcactc cggaccacct
     8281 cgacctcgcg ccagatggag gccttcccca ccttgagcgc cttggcgaac gcctccttgg
     8341 cggcgaagcg gacggcgaag gactcggcct ggcgggcgcg cctcaagcag tagtcgatct
     8401 cgccgggggt gtagatgcgc tcgacgaact tgtcccccca ccggaggatg gcttccgcga
     8461 tgcgcctcac ctcgacgatg tcgacgccga ccgaaaccac gcgggggtca gtccctgccg
     8521 ttcagcccgt cgacggccga caggagcgtt ccgttcagct catcgacgat cgcgaccagc
     8581 tcgtagacgt cgttcagctc gtaatcggcc ccggagttca ccgtcccgaa ggtgtcgccg
     8641 tagcgcgcaa aggccgtgcg aaggccgacc tgcgcggctc cgaccacgtc ccgttccggc
     8701 cagtcgccga ccatgatggc ctcctgcggc tggatgccga gcatctcgat cgcgcgctgg
     8761 aagggtttcg ggctcggctt gcgctccccc gtgtcctcga aggccaccac ggtgtcgaag
     8821 aggtgatgga gctggagatg ggcgagccgc atgtacgcct ggagcctggg agcatcggag
     8881 accacggcga gcttgagccc ccgcttcagg agctcgagca gggtcaggtt cacgtggggg
     8941 tagaggacga gataggagtc gcgggcccgc cggtaggcca cgatgcccga gctgaggatc
     9001 ttgtagtcga cccggcccag agcctccttc aggaagatgt cgaagacctg ctggtactcg
     9061 atcccctcgc ggtcgtagac ctgatagatg cgcgaggcga cctcgtcgcg cgggagggcg
     9121 aggccggcgt cgcgcatccc gtcgacggcg gcgtcgatcg cggcccgctt catcttcatg
     9181 aagtcggtca gcgtgttgtc gaggtcgaag atgatggctc ggatcacgat cgttccgatg
     9241 ggccaaggtc cgcccgcgga agcttgcgcg gggcgggtcg cgtcagaggc tgcgagcgaa
     9301 gctacttgct cgacggaccg atagcgcggc ccagactgat ctcaccgttg tcgatgcgga
     9361 cgttgtagcc gcaggtgggg ttggtgcaaa cccaagcttt gtaggtgatc ggggcgccgt
     9421 ccctgccgta gtccgaaagc ggtatcaaga ctcccgaaga acacttccgg cactcaggaa
     9481 acttgatttc catacggttg tccccctcca cacgaaatcc ccggcgaggc gcggctggtc
     9541 cacctcgccc ccaagagccg atcgtccact gggcccaagg gggtgtcaag cacaaatcac
     9601 gtcatttcaa tggaatggat gcgctatcct g
//
LOCUS       DASTKC010000209         6902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2062132, whole genome shotgun sequence.
ACCESSION   DASTKC010000209 DASTKC010000000
VERSION     DASTKC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062132"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..723)
                     /locus_tag="VFR25_08710"
     CDS             complement(112..723)
                     /locus_tag="VFR25_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET9941173.1"
                     /translation="MSKHPVKPLPYANDALKGIGSKTIEIHHDKLYAGYVNKRNEIEE
                     ALAKVDRSKANQIYSEMRGLKHEETFAANGQILHEIYFSIMGGDGNPAGEVVDKLKED
                     FGTFAAWEEDFKASAMCARGWVVLAYDPTDGRLHNFIGDSQNQGGVWGTFPILNCDTY
                     EHSYFIDYGSDRKAYVEAFMRNINWTEVNRRLAAAVQMQMARK"
     gene            complement(814..3483)
                     /locus_tag="VFR25_08715"
     CDS             complement(814..3483)
                     /locus_tag="VFR25_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET9941174.1"
                     /translation="MMDADFRKYLAAVKADEPPAMSTDPRWQETEALARRLIAESKVE
                     AALKSLEQEPLRRKRWDLLLLNALLREGLGEYAAALESFEVVADKLMASGDRDGVRAL
                     LDRFLEPEPTTPAVRILVFLARAPGLSESERLDLLREAIEIRPGDPDLNGEVAAALEH
                     AGDADGARQHRLRSIELHLEAGHAERVSDDFLRLIEEDLEHHPARVGRALLRFASLVS
                     FAESETLLDLAVPELKQRAPGLLSWDDIAPMAPRVPANATGRKILAALLRIAVAREPE
                     PDAILEGSGIADATKPLDQVAARLPKILALPPGAHVGHSTWGLGRVRASDGENLTLDF
                     PSRAGHRMSFAMASKSLDRLPGDGLRVLAAEDSARARALAAAGDPEILVRVLRDVGGT
                     ATAAQLKPKLEAVLPGLDVGAYWKQSKDAIKGDARLDSSEAYRQIYRLAPEGAAAGGA
                     VLPQLQPKAATQGLGLIRKFLREHPDEEPRFRETAGPLVKRWSGDDTLDAPTRAQALC
                     YALTWETVGETAAREHLEALIRKGLWPDDLALSANQDQLLDLASGSKLEEDFLWRAAE
                     SRLPRLRQRARARLKQLLGARFPKAVENKITRWADAPGLAARLIEHYAAHPDEAEAPP
                     LGMLTVATLRLLEKDIPEGAPERLLALLEDKGALQTRLRTEPPDEDTRSLIENVVLNW
                     SGSERRLVPVLEAMRAIGMGSLAEEYESRRKARAQSLLEGKTTDDVETVFIVMTRPTY
                     DRLEAELRRIALELKTTIPAAIEKARQLGDLRENAEYEAAKLKQANAAARLQELMTTL
                     ERTRLLETMEIDPSRIGVGTEATLKPADGNGGTITYWLLGDGDSGLAPGVLSYRAPLA
                     RPLLGKTVGSYVDLEYPTGTRRFLVESIVKRLP"
     gene            3663..4556
                     /locus_tag="VFR25_08720"
     CDS             3663..4556
                     /locus_tag="VFR25_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9941175.1"
                     /translation="MIAALGFNQIFSSLTHIIAKGAVAAIGPLAVALIRFTIASFTLL
                     VYQRARGGMSVAREDRIKLVMLGFFVVPINQGFFLFGLSRSTASHASLLYALTPLFVL
                     LLAGRVLGERSIGSKLFGTLVAFAGVAIILFERGLRNEMGVLVGDVMILIAVSAWAAY
                     TVLAKQLVERYDAMTVTTWAIVSGTVMCLPGFLVPGAIPPLKSIGPGAWGGILYLSIG
                     TSVIAYPLYMYALRHLEASKVAVTTNIQPILTAILSWVIFGERFTPGFVMGALLVLAG
                     VTWVETRKSPAPAPVPAEALR"
     gene            4556..5863
                     /locus_tag="VFR25_08725"
     CDS             4556..5863
                     /locus_tag="VFR25_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9941176.1"
                     /translation="MRVAALRGDELPEIRTAVPGPKSIAAAKRLRRAEGAAIWGVDSQ
                     PIVWARAKGAVVEDLDGNRFLDLTSGFGAASLGHATPEIKRAVSAQAGRLVQGLGDLM
                     PHRERERLVRALSRVGGRLDRVLLASTGSEAVELALKTAALATGRRRVVAFTGSYHGD
                     SLGALEVTHHPAPPGPIQALLGGWAVHVAYGDLDSLERALREGEPCAAVIAEPIQGRG
                     GTVIPPPGFLAGLCALARKHGAVVIYDEVMTGAGRTGPFWAWERSGPEAEPDLLCAGK
                     GIGGGVAIAALFGRPKLMDAWRRHVPASGEAPHASTFYAHPLACAGALATLKRLTAPG
                     LREEVERKGRWLDDALTKLARRYGAVVRSTGLMAGIELPSDGSPGSVEARLASLWRGL
                     LERGVLTVPSGTHGSVLQLLPPFTITDEQLAHGVAAIDSVLAG"
     gene            complement(5877..>6902)
                     /locus_tag="VFR25_08730"
     CDS             complement(5877..>6902)
                     /locus_tag="VFR25_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase domain-containing protein"
                     /protein_id="HET9941177.1"
                     /translation="EVYLYVCLIDFFERQNGKKSLDYEALYKDVREMIDEAHRDGSLK
                     SVITGNLDRYIRLDPEVRTVLEEFRRAGKKLFLLTNSEWSYTDALMRHILGRGKGAPE
                     HWSDLFNVVIVEAGKPQFFVTGDAPAPVDELQGRKVSAMVGRGGGATYLQRTLGSGGE
                     HVLYFGDHTYGDILQSKRVANWRTAMIVPELDREITVTAEHAKEFDRLARLSSERHQL
                     EIDKAAISRELRRLTLILSEQDGADPARRAEIAARIQEIPEQIAGFEHRGSELEHEIG
                     QLRLKIDRSYNPRWGSLFREGNESSRFGHQLKDFACVYTSRVSNFLHYPWNYYFQSPV
                     GYMPHDI"
BASE COUNT          957 a         2347 c         2393 g         1205 t
ORIGIN      
        1 agtcatgcgg gcatcgatgg cttcgcgagt gaagccatac agcttcgagc aaacgaaagc
       61 gggcgccgtg cgttttgcac gcgcgcccgc cggactgcaa cgtgccgtcg tctacttcct
      121 ggccatctgc atctggaccg cggccgcgag ccgccggttc acttcggtcc agttgatgtt
      181 gcgcatgaac gcctcgacgt aggccttccg gtcgctgccg tagtcgatga agtaggagtg
      241 ctcgtacgtg tcgcagttca ggatcgggaa ggttccccac acgccgccct ggttctgcga
      301 gtcgccgatg aagttgtgga gccggccgtc ggtgggatcg tacgcgagca cgacccagcc
      361 gcgcgcgcac atggcggacg ccttgaagtc ctcctcccac gccgcgaacg tgccgaagtc
      421 ttccttgagc ttgtcgacga cctcgccagc cggattaccg tcaccgccca tgatcgagaa
      481 atagatctcg tggaggatct ggccgttggc ggcgaaggtc tcctcgtgct tcaggccgcg
      541 catctcgctg tagatctggt tggccttgct ccggtcgacc ttcgcgagcg cttcctcgat
      601 ctcgtttcgc ttgttcacgt agcccgcgta gagcttgtcg tgatggatct cgatggtctt
      661 ggatccgatc cccttgagcg cgtcgttcgc gtaggggagc ggcttcacgg gatgcttgga
      721 catgaggcgc gtctcctttc gacgggtgcc ccggagctgc gtccgggcgc cacagggtga
      781 ttcgctggtt ggagccccat aatatcagaa tcgctagggg agccgcttga cgatggattc
      841 gacgaggaag cgccgcgttc ccgtgggata ttcgaggtcc acatacgatc ccacggtctt
      901 tccgaggagc gggcgcgcga gcggcgcgcg gtaggagagg acccccggcg cgaggcccga
      961 gtcgccgtcg ccgaggagcc agtaggtgat cgtcccgccg ttcccgtccg ccggcttgag
     1021 cgtggcctcg gtgccgacgc cgatgcgcga cgggtcgatc tccatggtct cgaggagtcg
     1081 cgtgcgctcg agcgtggtca tgagctcctg caagcgagcg gccgcgttcg cctgcttcag
     1141 cttcgcggct tcgtactcgg cgttctcgcg aagatcaccg agctgccgcg ccttctcgat
     1201 cgcggcgggg atcgtggtct tgagctcgag ggcgatgcgc cggagctccg cctcgagccg
     1261 gtcgtaggtc ggtcgcgtca tcacgatgaa cacggtctcg acgtcgtcgg tcgtctttcc
     1321 ctcgaggaga ctctgggcgc gggccttgcg ccgcgactcg tactcctcgg caagcgagcc
     1381 catgccgatg gcgcgcatgg cctcgagcac cgggacgagg cgccgctcgc tcccactcca
     1441 attgagcacc acgttctcga tgagtgagcg cgtgtcttcg tccggcggct ccgtgcggag
     1501 ccgcgtctgg agcgcgccct tgtcttcgag cagcgcaaga aggcgttcgg gcgctccctc
     1561 cgggatgtcc ttctcgagca ggcggagcgt cgcgaccgtg agcatcccga gcggaggcgc
     1621 ctcggcctcg tcgggatgcg ccgcgtagtg ctcgatcagc cgcgcggcga gccccggcgc
     1681 gtcggcccag cgggtgatct tgttctcgac cgccttgggg aagcgcgcgc cgaggagctg
     1741 cttcagccgg gcgcgcgcgc gctgcctgag tcgtgggagc cggctctccg ccgcgcgcca
     1801 gaggaagtcc tcctcgagct tcgaccccga ggcgagatcg aggagctgat cctgattcgc
     1861 gctcagcgcg aggtcgtcgg gccagagccc cttccggatc aacgcttcca agtgttcgcg
     1921 cgcggccgtc tcgccgaccg tctcccatgt gagcgcatag cagagcgcct gcgcgcgcgt
     1981 cggcgcatcg agcgtgtcgt cgcccgacca tcgcttgacg agcggcccgg cggtctcgcg
     2041 gaaacggggc tcctcgtcgg gatgctcgcg caggaatttc cggatcaagc cgagcccctg
     2101 ggtcgccgcc ttcggctgga gctgagggag cacggcgcca ccggccgccg cgccctcggg
     2161 cgcgaggcgg tagatctgcc ggtaggcctc gctcgagtcc agccgggcat cacccttgat
     2221 cgcgtccttc gactgcttcc agtacgcgcc cacgtcgagc ccgggaagca ccgcctcgag
     2281 cttcggcttc agctgggccg cggtggccgt gcctcccacg tcgcggagca cccgcacgag
     2341 gatctcggga tcgccggccg ccgcgagggc gcgcgctctg gcggaatcct cggcggccag
     2401 cacgcggagc ccgtcgcccg gaaggcggtc gagcgacttg gacgccatgg cgaaggacat
     2461 gcgatggccg gcgcgcgaag ggaagtcgag cgtcagattc tctccgtcgc tcgcgcgcac
     2521 tcgccccagc ccccatgtgg agtggccgac atgggcgccc ggaggaagcg cgagaatctt
     2581 cgggagccgc gcggccacct gatcgagcgg cttcgtcgcg tccgcgatgc cggagccctc
     2641 gaggatcgcg tcgggctctg gctcccgcgc gaccgcgatg cgaaggagcg ccgcgaggat
     2701 cttccgtccc gtcgcgttgg cgggaacgcg cggggccatc ggcgcgatgt cgtcccagga
     2761 gaggaggccc ggcgctcgct gcttgagctc gggcaccgcg aggtcgagga gcgtctccga
     2821 ctcggcgaag gacacgagcg acgcgaatcg caggagcgcg cgtcccaccc gcgcgggatg
     2881 gtgttccagg tcctcctcga tcagccggag gaaatcgtcc gacacgcgct cggcatggcc
     2941 tgcctcgaga tggagctcga tggagcgaag ccggtgctgc cgcgctccgt cggcgtcgcc
     3001 cgcgtgctcg agcgcggccg cgacctcccc gttcaggtcg ggatccccag gccggatctc
     3061 gatcgcctcg cgaaggagat cgagccgctc cgactcggaa aggccgggtg cccgagcgag
     3121 gaagacgagg atccgcaccg ccggcgtcgt gggctccggc tcgaggaagc ggtcgaggag
     3181 cgcgcgcacg ccgtcccggt cgcccgacgc catgagcttg tccgcgacca cctcgaacga
     3241 ctcgagcgcg gctgcgtact cgcccagccc ttccctgaga agggcgttga ggaggaggag
     3301 atcccagcgc ttccggcgga ggggttcctg ctcgagtgac ttgagcgcgg cttcgacctt
     3361 gctctcggcg atcagccggc gggcgagggc ttcggtctcc tgccagcgcg ggtcggtgga
     3421 catggccggc ggctcgtccg ctttgacggc ggcgaggtac ttcctgaaat cagcgtccat
     3481 catgcgttcg ctcctccaag cccctcagga taccgcaagc gcgctcatcc cggccagtct
     3541 cgtcccgcga gacggtccgc ggactcgttg cgcggcacgc cctcgtcgac tagaatccgc
     3601 ccttggaccg cacgacgagc ggtggatcgg gccccggggc gccgcgcgcg ccgaaatcgc
     3661 gggtgatcgc cgccctcggc ttcaaccaga tcttctccag cctcacccac atcatcgcga
     3721 agggcgcggt ggccgccatc ggcccgctcg ccgtcgccct catccgattc accatcgcct
     3781 cgttcacgct gctcgtgtac cagcgcgcgc gtggcgggat gtccgtcgcg cgcgaggaca
     3841 ggatcaagct cgtgatgctc ggcttcttcg tggtgccgat caaccagggc ttctttctct
     3901 tcgggctctc gcgatcgacg gcgagccacg cgtccctcct ctacgcgctc actccgctct
     3961 tcgtgctcct cctggctgga agggtgctcg gcgagcgctc gatcggctcg aagctcttcg
     4021 gaacgctcgt cgcattcgcg ggtgtcgcga tcatcctgtt cgagcgtggt ctccggaacg
     4081 agatgggggt tctcgtgggg gacgtcatga tcctgatcgc ggtgtccgcg tgggccgcct
     4141 acacggtgct cgcgaagcag ctcgtcgagc gctacgacgc catgacggtg acgacgtggg
     4201 ccatcgtcag cgggacggtg atgtgccttc ccgggttcct ggttccgggc gccattccac
     4261 cgctcaagtc gatcggcccc ggagcgtggg gcggcatcct ctatctctcc atcgggacct
     4321 cggtgattgc gtaccctctc tacatgtacg ccctccgcca tctcgaagcc tcgaaggtcg
     4381 cggtgacgac gaacatccag cccatcctca ccgccatcct ctcgtgggtc atcttcgggg
     4441 aacggttcac gcccgggttc gtgatgggcg cgctcctcgt gctcgcaggc gtgacgtggg
     4501 tcgagacgcg caagtccccg gctccggctc cggtgccggc ggaggctctc cggtgatgcg
     4561 cgtggccgcg cttcgagggg acgagcttcc cgagatccgc accgcggtgc cgggtcccaa
     4621 gtcgatcgcc gcggcgaagc gtctccggcg cgcggagggg gccgcgatct ggggcgtcga
     4681 ttcccagccg atcgtatggg cgcgcgccaa gggcgcggtc gtcgaggatc tcgatgggaa
     4741 tcgcttcctc gatctcacgt ccgggtttgg cgcggcgtcg ctcgggcatg cgaccccgga
     4801 gatcaagcgc gcggtctcgg cgcaggcggg ccgtctcgtg caagggctcg gagatctcat
     4861 gccgcatcgc gagcgggaac gcctcgtacg tgcgctgtcg cgcgtgggag gccgcctcga
     4921 tcgggtgctc ctggcgtcca ccggctccga agcggtggag ctcgccttga agacggccgc
     4981 tctcgccacc ggacgaaggc gtgtggtggc gttcacgggc tcgtatcacg gcgactccct
     5041 gggcgcgctc gaggtcacgc atcatccggc gcctcccggt ccgatccagg cactgctcgg
     5101 cggttgggcg gttcacgtcg cctacggcga tctggactcc ctcgagcgcg cactgcgcga
     5161 gggcgagccc tgcgccgccg tgatcgcgga gccgatccag ggacgtggcg gcacggtgat
     5221 tccgccgccc ggctttctcg ccgggctctg cgcgctcgcg cgcaagcacg gcgcggtcgt
     5281 gatctatgac gaggtgatga ccggcgccgg gcggaccggc cccttctggg cctgggagcg
     5341 ctccggtccc gaagccgagc ccgatctcct gtgcgccggc aaggggatcg gcggcggagt
     5401 cgcgatcgcg gcattgttcg gtcggccgaa gctcatggac gcgtggcgcc ggcacgtgcc
     5461 cgcgtccggc gaggcgccgc acgcgagcac gttctacgcg cacccgctcg cctgcgcggg
     5521 cgcattggcc acgctgaagc ggctcacggc gccagggttg cgcgaggagg tcgagcggaa
     5581 ggggcgctgg ctcgacgacg cgctcaccaa gctggctcgg cgctacggtg cagttgtccg
     5641 ctcgacggga ttgatggccg ggatcgagct tccgtccgat ggatcgccgg gctcagtaga
     5701 ggctcgtctt gcgtcgctat ggcgcggact tctggagcgt ggtgttctca cggttccgag
     5761 cggcacgcac ggaagcgtgc tccagctcct cccgcccttc acgatcacgg acgagcagct
     5821 cgcgcacggc gtcgccgcga tcgattcggt tctggctggc tgagtcgaag agccgactag
     5881 atgtcgtgcg gcatgtagcc gacgggggac tggaagtagt agttccaggg gtagtggagg
     5941 aagttcgaga cccggctcgt gtagacgcag gcgaagtcct tgagctggtg gccgaagcgg
     6001 ctcgactcgt ttccctcgcg gaacagggat ccccagcgcg gattgtacga gcggtcgatc
     6061 ttgaggcgga gctgcccgat ctcgtgctcc agctcggacc cgcggtgctc gaaccccgcg
     6121 atctgctcgg ggatctcctg gatgcgcgcc gcgatctcgg cccggcgcgc cggatccgcg
     6181 ccgtcctgct cgctcaggat caaggtcagc cgccttagct cgcggctgat cgcggccttg
     6241 tcgatctcga gctggtggcg ctcggacgac agccgcgcga gccgatcgaa ttccttcgcg
     6301 tgctcggcgg tgaccgtgat ctcgcggtcc agctccggca cgatcatggc cgtgcgccag
     6361 ttggcgacgc gcttggattg caagatgtcg ccatacgtgt gatccccgaa gtagagcacg
     6421 tgctcgccac cggagccgag cgtccgctgg aggtacgtgg cgcccccgcc gcgccccacc
     6481 atcgccgaga ccttccgccc ctggagctca tccaccggag ccggcgcgtc gccggtcacg
     6541 aagaactgcg gcttgcccgc ctccacgatc acgacgttga agagatcgct ccagtgctcg
     6601 ggcgccccct tgccgcgccc gaggatgtgg cgcatcaagg cgtcggtgta ggaccactcg
     6661 ctgttcgtga ggaggaagag cttcttcccg gcgcggcgaa actcctcgag caccgtccgc
     6721 acctcggggt cgaggcggat gtaacggtcc agattgcccg tgatgaccga cttcaagctc
     6781 ccgtcccggt gcgcctcgtc gatcatctcg cggacgtcct tgtagagcgc ctcgtagtcg
     6841 agcgacttct tcccgttctg gcgttcgaag aagtcgatca ggcagacgta gagatagacc
     6901 tc
//
LOCUS       DASTKC010000210         7489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_643048, whole genome shotgun sequence.
ACCESSION   DASTKC010000210 DASTKC010000000
VERSION     DASTKC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643048"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VFR25_08735"
     CDS             <1..1583
                     /locus_tag="VFR25_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9941178.1"
                     /translation="LNRNYSFLWGWDNIGSSPTPSSDVYRGTAAFSEPETAALRDFIN
                     AHDFTVSASFHSYGELFLYPWGYALLNTPDQYIFESFGDSVSVQNGYRAGNPENGAIY
                     LTNGSLDDWLYGDASQRPKVFGFTFEMNTEAQGGFDPSDALIQPTCEKNWGPVLTLLR
                     YADAPRRIIPPARAAAPTFVASPPGELELYWTYPVPDPVNPVISEEVLRIASLGQGVD
                     DAESGVARWDSTGFTWSTARHASGARSFYSGTGNMVQSSLTSWYAPDVAAGDSLVVTA
                     YWDTELDYDYWYADASYDGGATWLSLHGNRTTTFDPNGTNEGNGVTGSSGGAFLRASF
                     LLPSAGQPRVRFRYATDGVTFGEGVYLDDIGPTAFESGLTVNAMSGAPGHWHLNPAPS
                     APTWFEVRGIDPEGQRGPWGTRVRFDPVVSGVAAAPPPAVDRLGSNAPNPFNPRTEIP
                     FALGAGVAGRYRLAVYDLAGRRVAVLAEGWDDGTGAARRAVWDGSDGGGRSMGSGVYL
                     VRLESVRGASSRKITLLR"
     gene            1839..2759
                     /locus_tag="VFR25_08740"
     CDS             1839..2759
                     /locus_tag="VFR25_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9941179.1"
                     /translation="MRSRHRARGVRITGAALVAAALASCSSPPPPRSAHPGPLKLAGT
                     EYVAPLLRAEILAFRDRYPDADSIQLMANGSAEGMEQLVNGEVSMSLLLRDLTDPEAD
                     AAVKRDGLQAFSVAWDAVAVIVNPASPVEQISKTELGSIYAGEIHAWGPLGWKAGGDL
                     IALTSGPRLGIYGYIEQTLLNDRPYAPTIYAPPSEEEVVGVVATRRNAIACVSRPVAE
                     AAGAKVKLLRVSQALGLPYVALDRETLLLKRYPLLRAISIATPAQPAETAGNFINFVS
                     GIDGQQIVARHGYAPATVPIRIVRTAEEAE"
     gene            2756..4594
                     /locus_tag="VFR25_08745"
     CDS             2756..4594
                     /locus_tag="VFR25_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941180.1"
                     /translation="MKLVTRIAVPLAFLALAGIASLSFALTSGDMIKQGVSLLREGKT
                     AEALDLFSRAQRLDPQGFKPHYYIASALERMGEPDSAAVEYQTALRINPKYVEALTGY
                     GKLLRKQGKKDEGTAKLEAAVKQDPKDAPALYALGTSYLEDKKFDDAERVMRKGTLLK
                     QGRALFLAGTALALEGKGELKQAEELFIRARETDPNNLRVRLDFGGFYDRKKIPVLAA
                     PEYAQAIQLEPKNPETHYRYGMALVGMNEFNSGLKAFIDATQQDSTYAPAYLEAGRLY
                     YRGKRYKDAADQFRVYTGLKPDDYQGYLELGQSLASSRDANDHIEATVYLTKANDMKP
                     DVPAVLGSLCKLYAEQGEAGRDSAVSYCDRYANAADSLTAEEENRIGSLYAQMGDSAN
                     AVLHLNKAAAQDSTYRMNAYFNLGFLFFAKQDFASSVPYFEKALAADSVFLPALLNIG
                     LAKLQLQQRPEAIYYLRRALLVKPEARTMVWIGQTMLQMEPDSLPVALQTFRDAATLD
                     PKNGDALRGAGLALLLMDQCTDAMDYLKKATDVDDQHVQGHIWLAQANVKCSNLPAAK
                     VEYNKAIEIDPNNREASRGLELIRQYEERKAQAGKAPAKPPVTKKN"
     gene            4638..4970
                     /locus_tag="VFR25_08750"
     CDS             4638..4970
                     /locus_tag="VFR25_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9941181.1"
                     /translation="MDVRTSSDVTILTPKGMLLGGKETDELQGKIKELGEAGNRKLLV
                     NLGQTTFLNSVSLGVLIAGHSSYAKRDAQMKLCAVDKKIQNIFVVTKLSLVFDVYDTE
                     AQALASFK"
     gene            5111..5833
                     /locus_tag="VFR25_08755"
     CDS             5111..5833
                     /locus_tag="VFR25_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9941182.1"
                     /translation="MRQGLFITIVAVAALTIAIVVYMFLPEYIKSGGPLVAGLIMLSI
                     MSVTLVFERLFTISRARGKDSIPAFMRAVRKKIDANDIEGAADLCSKQRGSLANVLRA
                     GLDRYQVVRTAQIDRKSKVDEVQKAMEEANMLEVPLLERNLIALATIASIATMVGLLG
                     TVIGMIRSFAALGHTGSVDAVKLAVGISEALINTAGGLFVAIFSIVLYNVFVTMIDNF
                     NYMMDEATLEVVEVISLKEATK"
     gene            5835..6275
                     /locus_tag="VFR25_08760"
     CDS             5835..6275
                     /locus_tag="VFR25_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9941183.1"
                     /translation="MSAKKMKRIGVKIDMTPMVDVAFLLLIFFMSTSQFDPPQKVPIV
                     LPDSHSNLKVPESDVLILGISKDSRLFWQVGKNPQEETDLPNLETLVLEQRRKNPRLR
                     VAIKADKDSQYGLMEDVMAVMQKTNTITFSLVTELVASKKSAMR"
     gene            6355..6819
                     /locus_tag="VFR25_08765"
     CDS             6355..6819
                     /locus_tag="VFR25_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9941184.1"
                     /translation="MRKRAPKRIGVKIDMTPMVDVAFLLLIFFMCTTVFRKPQALEIN
                     LPPDPNAKVEIAESNVLTLRVIAPADTSLGVRTFWALGKTPYQESKFDKLESIFAAEG
                     KKNPKLVVLVKIDRLTKYKYMVNIIDQLQFAELNRFSLAPLTPEEKKEVETL"
     gene            6816..>7489
                     /locus_tag="VFR25_08770"
     CDS             6816..>7489
                     /locus_tag="VFR25_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9941185.1"
                     /translation="MSATATAFDPTSTLDRVPIGAADLKANSQRMLRKALIVSAAIHL
                     GLIGLYMASQLYHPPDEAAYTGRVIKMQTLPPPPPLSSAPPPPVIPVNTEIVKPTIGT
                     PVPVPDAMAPEQTIMTQQEIAASLAPVGMGSGSGKDSLVIASGDDLPSEGEFVYYEDE
                     PVPVTAPPPAYPDFARDAGITGKVTLHVLVGKDGRVKNVKVIKGVTGLDAAAVDAVKK
                     WVFKPAL"
BASE COUNT         1358 a         2465 c         2445 g         1221 t
ORIGIN      
        1 acctgaaccg gaactactcc ttcctgtggg gatgggacaa catcgggtcg agccccacgc
       61 ccagctccga tgtgtaccga ggcaccgccg ccttctcgga gcccgagact gcggcgctcc
      121 gcgatttcat caacgcgcac gacttcaccg tctcggcgtc gttccacagc tacggcgagc
      181 tcttcctcta tccatggggc tatgccttgt tgaacacgcc ggaccagtac atcttcgaga
      241 gcttcggcga ctccgtgtcg gtgcagaacg gctatcgcgc cggcaatccg gagaacgggg
      301 ccatctacct cacgaacggg tcgctcgacg attggctcta cggtgacgcc tcgcagcggc
      361 cgaaggtctt cgggttcacg ttcgagatga acacggaagc acagggcggt ttcgacccat
      421 cggacgccct catccaacca acgtgcgaga agaactgggg accggtgctc acgctcctcc
      481 gctacgcgga cgcaccccgc cggatcatcc ctcccgcgcg ggcggcggcg cccacgttcg
      541 tcgcatcgcc tccgggcgag ctggagctct actggaccta tcccgttccc gatccggtca
      601 acccagtgat ctcggaggag gttctcagga ttgcctcgct ggggcagggc gtggacgacg
      661 cggaatcggg cgtcgctcgg tgggactcga ccgggttcac atggagcacc gcgcgccacg
      721 cgagcggggc gcgcagcttc tactccggaa ccggcaacat ggtccagagc agcctgacct
      781 cgtggtacgc ccccgacgtc gcggcggggg acagcttggt ggtcacggcc tactgggaca
      841 ccgagctcga ctacgactac tggtacgcgg acgcgtccta tgacggcgga gccacgtggc
      901 tctccctcca tggcaaccgc accaccacgt tcgacccgaa tgggacgaac gaggggaacg
      961 gggtcaccgg atcaagcggt ggtgcgttcc ttcgtgcatc gttccttctc ccgagcgcgg
     1021 ggcagcctcg cgtgcgcttc cggtacgcca ccgacggcgt gaccttcggg gagggcgtct
     1081 acctggacga catcgggccg accgcattcg agtctgggct gacggtgaac gccatgtccg
     1141 gggcgcccgg ccactggcac ctcaaccccg ctccctcggc accgacctgg ttcgaagtgc
     1201 ggggtatcga ccctgaaggc cagcggggtc cgtggggcac acgcgtgcga ttcgatcctg
     1261 tcgtgagcgg tgtggccgcc gcaccaccgc cggccgtgga tcggctcgga tcgaacgcgc
     1321 cgaacccgtt caatccgagg accgagatcc cgttcgcact cggggccggg gtggcagggc
     1381 gctaccggct cgccgtgtac gacctcgcgg gacggcgcgt cgccgtgctg gccgagggct
     1441 gggacgacgg aaccggggcc gcgcggcgcg ccgtgtggga cggctccgac ggcgggggac
     1501 ggtccatggg aagcggcgtc tacctggtcc ggctggaatc ggtccggggg gcttcgtcga
     1561 ggaagatcac cctgctccgc tgaggtcccc aggcgtcggc gtggtttccc gttgacactt
     1621 agtccaccct cccttacgtt ccctcctcgc ttcgacgcta gcgtgcgcgg ccaccctgcc
     1681 cgggtggcgt ttttgcgctt ggcggacgct ggctcgccac ctttggttac ggggaattaa
     1741 ctgccgtcat ccggggtatg cagggtaggt tgaacgcgaa gcatggcggg gaagggccga
     1801 cgattgacag gcttggcagg ggccggtagg ctggccccgt gcgtagtcgt caccgggcgc
     1861 gcggcgtccg gattactgga gcggcgctcg tcgccgccgc cctggcctct tgctcgtcgc
     1921 cacccccccc tcgcagtgcc catccaggac cgctcaagct ggcgggaacc gagtacgtgg
     1981 ctccgctttt gcgggccgaa atcctggcct ttcgggaccg gtatccggac gccgactcca
     2041 ttcagctcat ggccaacggg tcggcggaag gaatggagca actggtgaac ggtgaggttt
     2101 cgatgtctct cttgctgcgg gatctgaccg atccggaggc tgacgccgcc gtgaagcggg
     2161 acggcctcca ggcgttctcc gtcgcgtggg acgccgtcgc cgtgatcgtg aatccggcgt
     2221 cgcccgtcga gcagatctcg aaaacggagc tgggctcgat ctacgcgggc gagatccacg
     2281 catggggacc gctcggctgg aaggcgggcg gcgatctcat cgcgctcacc tcgggtcccc
     2341 ggctcggcat ctacggctac atcgagcaga cccttctcaa cgaccgtccc tacgccccga
     2401 ccatctacgc cccaccgtcc gaagaagaag tggtcggcgt cgtggccacc cgccggaacg
     2461 cgatcgcctg cgtgtcgcgg ccggtcgcgg aggcggcggg cgccaaggtg aagctgctcc
     2521 gcgtctcaca ggcgctgggc cttccgtacg tcgcgctcga ccgggagacc ctgctcctga
     2581 aacgctatcc gctcctgcgc gcgatctcga tcgcgacgcc cgcccaaccg gccgagacgg
     2641 ccggcaactt catcaatttc gtctccggaa tcgacggcca gcagatcgtc gcgcgccatg
     2701 gctacgcgcc ggccaccgtc cccatccgga tcgttcgtac cgcagaggag gccgagtgaa
     2761 gctcgtgacc cgcatcgccg ttccgctcgc gttcctggcc ctggccggaa tcgcgtccct
     2821 ctcctttgcc ctcacgtccg gcgacatgat caagcagggc gtgtcgctcc tccgcgaagg
     2881 aaagacggcg gaggccctgg acctcttcag ccgcgcccag cggctggatc cgcagggctt
     2941 caagccgcac tactacatcg cgtccgccct cgagcgcatg ggcgagcccg acagcgccgc
     3001 ggtggaatac cagaccgcgc tccggatcaa tccgaagtac gtcgaagcgc tgaccggcta
     3061 tgggaagctc ttgcgcaagc agggaaagaa ggacgagggc acggcgaagc tcgaggcggc
     3121 agtgaagcag gatccgaagg acgccccggc gctctacgcg ctcggaacgt cgtatctcga
     3181 ggacaagaag ttcgacgacg cggagcgtgt catgcggaag gggacgctct tgaagcaggg
     3241 gcgcgccctc ttcctcgcgg gcaccgcgct cgcgctcgag ggcaaggggg agctgaagca
     3301 ggcggaggag ctcttcatcc gcgcgcgcga gacggatccg aacaatctgc gggttcgcct
     3361 cgacttcggc ggattctacg atcggaagaa gatccccgtc ctcgcggctc cggaatacgc
     3421 ccaggcgatc cagctggagc ccaagaatcc agagacccac taccgctacg gaatggcgct
     3481 ggtggggatg aacgagttca actccgggct caaggcgttc atcgacgcga cccagcagga
     3541 ctcgacgtac gcgcccgcct acctcgaggc ggggcgcctc tattaccgcg gcaagcgcta
     3601 caaggacgcg gcggaccaat tccgcgtgta cacgggtctc aagcctgacg actaccaggg
     3661 ctacctggag ttgggccagt ccctcgccag ctcgcgcgac gcgaacgacc acatcgaggc
     3721 cacggtctat ctgaccaagg cgaatgacat gaagccggac gtgcccgcgg ttctgggctc
     3781 tctttgcaag ctctacgcgg agcagggtga ggcgggccgc gacagcgcgg tcagctactg
     3841 cgaccgctac gcgaacgccg ccgattcgct cacggcggaa gaggagaacc ggatcggctc
     3901 cctgtacgcg cagatgggcg actccgcgaa cgccgtgctg cacctgaaca aggcggctgc
     3961 gcaggactcg acctaccgga tgaacgccta cttcaaccta ggcttcctct tcttcgcgaa
     4021 gcaggacttc gcgtcctcgg ttccgtactt cgagaaggcc ctcgcggcgg actcggtctt
     4081 ccttccggcg ctcctcaaca tcgggctcgc caagctccag ctccagcagc ggccggaggc
     4141 gatctactac ctgcgccgcg cactcctggt gaagccggag gcgaggacca tggtgtggat
     4201 cgggcaaacg atgctccaga tggagcccga ttcgctcccg gtcgcgctcc agaccttccg
     4261 cgacgcggcg acgctcgatc ccaagaacgg ggacgcgctt cgcggcgcgg gtctcgcgct
     4321 ccttctcatg gaccagtgca ccgacgcgat ggactacctg aagaaggcga ccgacgtcga
     4381 cgaccagcac gtgcaggggc acatctggct cgcgcaggcg aacgtgaagt gcagcaatct
     4441 cccggcagcg aaggtcgagt acaacaaggc catcgagatc gatccgaaca accgcgaggc
     4501 gtcgcgggga ctcgagctca tccggcagta cgaagagcgc aaggcgcagg cgggcaaggc
     4561 gcccgcgaaa ccgcccgtga cgaagaagaa ctagtcctac gcggcgtccg ccgcgcaatt
     4621 ggggaggcat gatgagtttg gatgtccgta cctcgagtga cgtgacgatt ctcacgccga
     4681 aggggatgct cctgggtggg aaggagacgg acgagctgca gggcaagatc aaggagctgg
     4741 gcgaggcggg gaaccggaag ctcctcgtca atctcggcca gaccacgttc ctgaactcgg
     4801 tgtccctggg cgttctgatc gcggggcact cgagctacgc caagcgggac gcgcagatga
     4861 agctctgcgc ggtggacaag aagatccaga acatcttcgt ggtcaccaag ctgtcgttgg
     4921 tgttcgacgt ctacgacacg gaggctcaag cgctggccag cttcaagtaa caggagttgc
     4981 cggtcccggg cccctggcgt atgctaggcg gccagccacg atccacgctc cggggccgga
     5041 ccggagaggg tcaccccatc gcatcgctcg aacgagcgga acgcacggtt cgaaccagga
     5101 ggaagtcgca gtgagacaag gtttgttcat caccattgtg gccgtcgcgg ccctcaccat
     5161 cgccatcgtc gtctacatgt tcctgcccga gtacatcaag tcgggcggtc cgctggtagc
     5221 cggcctcatc atgctctcga tcatgtcggt gacgctggtc ttcgagcgcc tcttcaccat
     5281 ctcccgtgct cgcggcaagg attcgatccc cgcgttcatg cgcgccgtgc ggaagaagat
     5341 cgatgcgaac gacatcgagg gcgccgcgga tctctgctcc aagcagcgcg gctcgctcgc
     5401 gaacgtgctc cgcgccggcc tcgaccgcta ccaggtcgtc cgcaccgcgc agatcgaccg
     5461 caagagcaag gtcgatgaag tgcaaaaggc gatggaagag gcgaacatgc tcgaggtgcc
     5521 gctgctcgag cggaacctga tcgcgctcgc gacgatcgcc tcgatcgcga cgatggtcgg
     5581 cctcctcggg acggtgatcg ggatgatccg gtcgttcgcg gcgctcggcc acaccggctc
     5641 ggtggacgcc gtgaagctcg ccgtcggtat ttccgaggcg ctcatcaaca ccgccggcgg
     5701 cctcttcgtg gccatcttct cgatcgtgct ctacaacgtg ttcgtgacga tgatcgacaa
     5761 cttcaactac atgatggacg aggcgaccct cgaggtcgtc gaggtcatct ctctgaagga
     5821 agcgacgaag taacatgtct gccaagaaga tgaagcggat cggcgtcaag atcgacatga
     5881 cgcccatggt cgacgtggcc ttcctgctcc tgatcttctt catgtccacg tcgcagttcg
     5941 acccgccgca gaaggttccg atcgtgctcc cggacagcca ctccaacctc aaggtcccgg
     6001 agtcggacgt cctcatcctc gggatctcga aggacagccg gctcttctgg caggtgggga
     6061 agaaccccca ggaggagacg gaccttccga atctcgagac gctcgtgctc gagcagcgac
     6121 gcaagaatcc ccggctgcgc gtcgccatca aggccgacaa ggactcccag tacggcttga
     6181 tggaagacgt catggcggtg atgcagaaga ccaacacgat cacgttcagc ctcgtcacgg
     6241 agctggtggc cagcaagaaa tccgcaatgc gctgatggcg catcctcaaa ggagtcgagt
     6301 gacatgggtg ccgtagacac cccagaagct ggcgtaaaaa agaaaaaagg agggatgcgg
     6361 aagcgcgccc ccaagcgcat cggcgtgaag atcgacatga cgccgatggt ggacgtcgcg
     6421 ttcctgctcc tcatcttctt catgtgtacg acggtgttcc ggaagccgca ggcgctcgag
     6481 atcaatctcc caccggatcc gaacgcgaag gtggagattg ccgagtcgaa cgtgctcacg
     6541 ctgcgggtca tcgcgccggc ggatacgagc ctcggcgtgc ggacgttctg ggcgctgggc
     6601 aagacgccgt atcaagagtc gaagttcgac aagctcgaat cgatcttcgc ggcggaaggg
     6661 aagaagaatc cgaagctggt cgtgctcgtg aagatcgacc ggctgacgaa gtacaagtac
     6721 atggtgaaca tcatcgatca gctgcagttc gccgagctga accggttctc gctcgcgccg
     6781 ctcacgccgg aggagaagaa ggaggtggag acgctgtgag cgccaccgcc accgctttcg
     6841 atccgacgag tacgctcgat cgcgttccga tcggagccgc cgatctcaag gcgaactccc
     6901 agcggatgct gcgcaaggcg ctcatcgtct cggcggccat ccatctggga ctcatcggcc
     6961 tctacatggc ctcgcagctc taccatccgc ccgacgaggc ggcgtacacg gggcgcgtca
     7021 tcaagatgca gacgctgccg ccgccgccgc ccctgtcgtc ggcgccgccg ccgcccgtga
     7081 tcccggtcaa caccgagatc gtgaagccga cgatcggtac gccggtcccg gttccggacg
     7141 ccatggcgcc cgagcagacc atcatgacgc agcaggagat cgccgcgagc ctggccccgg
     7201 tgggcatggg cagcgggtcc gggaaggact ccctcgtcat cgcgtcgggt gacgaccttc
     7261 cgagcgaggg ggagttcgtc tattacgaag acgagccggt gccggtcacg gcgccgcctc
     7321 cggcgtaccc ggacttcgcg cgcgacgccg gcatcaccgg caaggtcacg ctccacgttc
     7381 tcgtcggcaa ggacgggcgc gtgaagaacg tgaaggtcat caagggcgtc acgggtctcg
     7441 acgccgcggc ggtcgatgcg gtcaagaagt gggtgttcaa gcccgcgct
//
LOCUS       DASTKC010000211         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_171757, whole genome shotgun sequence.
ACCESSION   DASTKC010000211 DASTKC010000000
VERSION     DASTKC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171757"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2263)
                     /locus_tag="VFR25_08775"
     CDS             complement(<1..2263)
                     /locus_tag="VFR25_08775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9941186.1"
                     /translation="MPLDFFHPAVRQWFLERVGTPSAPQEQGWPLIAGGRNTLVAAPT
                     GTGKTLAAFLWALDGLLRQGPSLPDQTLVLYVSPLKALGNDVQKNLDAPLRELETRDP
                     SFARVRVLVRTGDTPSSQRASMTRKPPHILVTTPESLYILLTSQGGRAMLRTVRTVIV
                     DEIHAMAGNKRGAHLALSLERLEALVDGPLQRIGLSATQKPVDEVARVLAGVGRECAI
                     VDIGHRRDLDLAIEVPGSPLATVCSHEQWDKIVARAAELIQAHRTTLVFVNTRKLAER
                     IAAKLTAVLGEDKVTSHHGSLARERRLDAERRLKAGELRALVATASLELGIDIGDVDL
                     VIQIGETSSISVLLQRVGRSGHALGRTPKGRMFPLTQDQLVVATALLDSVRRGELDRL
                     HVPDRPLDILAQQIVAACVAETWDEAALHEAFRRAWPYRRLERAEFDDVVRLHTQGRA
                     ALLHRDGVNDRLRATKRASITALTSGGAIPDTGQYRVVLEPEGTLVGSLDEDFAVETN
                     GGDVFQLGNASWQILRVEPGVVRVADARGAPPTIPFWLGEGPARTDELAAAVGRVREQ
                     GRDTRWLTEEAGVLPAAATQLAEYLDEGARALGAVPTPECVVLERFFDESGGMQLVVH
                     APFGSRINRAWGLALRKRFCRGFGFELQAAANEEAIVISLGPQHSFPLEDVFQFLHVD
                     VARDLLVQALLDAPMFGTRWRWNLTRSLLVSRTQGGGKRVPTPLIRMRAEDELVRAFP
                     SALACPETMPPGDL"
     gene            2482..>2957
                     /locus_tag="VFR25_08780"
     CDS             2482..>2957
                     /locus_tag="VFR25_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941187.1"
                     /translation="MIRRLWTLLLTFLLVAASSEAATIRGTVRVPGTVSSSMAPSNPY
                     PGRASSMRASAVRRGAVTDAVVSIVGPGPGNWRGLPGDASASPLRGGRLAQKSQGFEP
                     RVLPVAVGGRVDFPNLDPIYHNVFSVSPPKRFDLGKYGQGKSKSLVFDKPGLVNVY"
BASE COUNT          427 a         1051 c         1036 g          443 t
ORIGIN      
        1 ggaggtcgcc cgggggcatc gtctcggggc acgcgagtgc gctcgggaac gctcgcacga
       61 gctcgtcctc ggcgcgcatc cggatgagcg gcgtcggaac gcgcttgccg cccccctgcg
      121 tccgggacac gaggagcgac cgcgtgagat tccagcgcca gcgggtgccg aacatcggcg
      181 catcgaggag cgcctgtacg agaaggtcgc gggccacgtc cacgtgcagg aactggaaca
      241 cgtcctcgag cgggaagctg tgctgtgggc cgagcgagat gacgatcgct tcctcgttcg
      301 ccgccgcctg gagctcgaac ccgaagccgc ggcagaaccg cttccgaagc gcgaggcccc
      361 acgcgcgatt gatgcggctc ccgaagggag cgtgcaccac cagctgcatg ccgcccgatt
      421 cgtcgaagaa tcgctccagc accacgcact cgggcgtcgg cacggcgccg agcgcgcgcg
      481 ctccctcgtc caggtactcc gcgagctgcg tcgcggccgc gggtaggacg cccgcctcct
      541 cggtgagcca gcgcgtatct cgaccctgct cgcgcacgcg acccacggcc gccgcgagct
      601 cgtcggtccg cgcgggccct tcgccaagcc agaagggaat ggtcggcggc gcgccccgcg
      661 catcggccac gcgaacgacc ccgggctcga cgcgcaggat ctgccacgag gcgttcccga
      721 gctggaacac gtcgcccccg ttcgtctcca ccgcgaaatc ctcgtcgagc gatcccacga
      781 gcgtcccttc cggctcgagc accacgcggt actgtccggt atcggggatc gcgccgcccg
      841 aggtgagcgc ggtgatcgac gcgcgcttgg tcgcgcgcag ccggtcgttc acgccgtccc
      901 gatggaggag cgccgcgcgc ccttgcgtgt ggagtcgcac gacgtcgtcg aactccgcgc
      961 gctcgagtct tcgatagggc caggcgcgac ggaaggcctc gtggagcgcg gcctcgtccc
     1021 acgtctccgc gacgcaggcg gccacgatct gctgcgccag gatgtcgagc ggccggtccg
     1081 gcacgtgcag gcgatcgagc tcgccccgcc gcacggaatc gaggagcgcc gtcgccacca
     1141 cgagctggtc ctgcgtgagc gggaacatgc gccccttcgg cgtgcggccg agcgcgtggc
     1201 cgctccgccc gacgcgctgg aggagcaccg agatcgacga tgtctcaccg atctggatca
     1261 cgaggtccac gtcgccgatg tcgatcccga gctcgagcga cgccgtcgcg acgagagccc
     1321 ggagctcgcc cgccttgaga cggcgctccg catcgagccg ccgctcgcgc gcgaggctcc
     1381 cgtggtggct cgtcaccttg tcctcgccga gcaccgccgt gagcttggcc gcgatccgct
     1441 cggcgagctt ccgcgtgttc acgaagacga gcgtggtccg gtgcgcctgg atcagctccg
     1501 cggcgcgcgc cacgatcttg tcccactgct cgtgggagca aaccgtggcg agcggggagc
     1561 ctggcacctc gatcgcgaga tcgagatccc gcctgtgtcc gatgtccacg atggcgcact
     1621 cgcggccgac gccggcgagg acgcgcgcga cctcgtcgac cggcttctgc gtggcggaga
     1681 ggccgatcct ctggagcggc ccgtccacga gcgcctcgag ccgctcgagc gagagtgcta
     1741 ggtgcgcgcc ccgcttgttg ccggccatcg cgtggatctc gtcgacgatg accgtgcgca
     1801 cggtccggag catcgcgcgc ccgccctggc tcgtgagaag gatgtagagc gattccggcg
     1861 tggtgacgag gatgtgcggc ggcttccgag tcatcgacgc gcgctggctc gacggcgtgt
     1921 cgccggtccg gacgagtacc cgtacccggg cgaaggaggg atcgcgcgtc tcgagctcgc
     1981 ggagcggcgc gtcgaggttc ttctgcacgt cgttcccgag cgccttgagc ggagagacgt
     2041 agaggacgag cgtctggtcg ggaaggctgg gcccctgccg gaggagcccg tccagggccc
     2101 agaggaaggc ggccagggtc ttcccggtcc ccgtcggggc ggccacgagg gtgttccgcc
     2161 cgccggcgat cagcggccag ccctgctcct ggggcgcgga gggtgtgccg acccgctcta
     2221 gaaaccattg gcgaacggca ggatggaaga aatcgagcgg cacggttgtc tcctcgatga
     2281 cgtcaggccg ggctcacatt ataccccgcg cgtcgcgggc ttggtcccag ggccggaacg
     2341 cgacgttcac ggccgggtag ccctcccgac ccggccaatg gcaaaagcct ttaagtccct
     2401 gtgcgagaac gccgatgggt gatttgcagg agcctccagg cgctccacga tccctccatc
     2461 gaagaggatg cgcgtcttgt catgatccgt cgcctttgga cgctgcttct cacgttcctt
     2521 ctcgtggctg cttcgagcga agccgcgacg atccgcggca ccgtgcgcgt gccggggacc
     2581 gtctcgtcgt ccatggcgcc gtcgaatccg tatccaggcc gtgcctcctc gatgcgcgcg
     2641 tccgccgtgc gccgcggggc cgtgaccgac gccgtcgtct cgatcgtcgg cccagggccg
     2701 ggcaactggc gcggcctgcc cggcgacgcg tccgcgagcc cgctccgcgg cggccggctc
     2761 gcgcagaaga gccagggctt cgagccgcga gtccttccgg tggcggtcgg gggcagggtc
     2821 gactttccca atctcgaccc gatctaccac aacgtgttca gcgtctcccc gcccaagcgc
     2881 ttcgatctgg gcaagtacgg ccaggggaaa tcgaaatcgc tcgtcttcga caagccaggc
     2941 ctggtgaacg tctactg
//
LOCUS       DASTKC010000212        11393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_409034, whole genome shotgun sequence.
ACCESSION   DASTKC010000212 DASTKC010000000
VERSION     DASTKC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409034"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1630
                     /gene="rho"
                     /locus_tag="VFR25_08785"
     CDS             320..1630
                     /gene="rho"
                     /locus_tag="VFR25_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9941188.1"
                     /translation="MEQQTESPPAVATGSPASPKGLDIIQLKGRTIAELMELAHSLGI
                     QGTSGLRKQELIFKILEGQTEKNGLIFAEGVLEILSEGYGFLRSPDYNYLPGPDDIYV
                     SPSQIKRFDLRTGDTVSGQVRPPKDGERYFALLRVEAVNFESPEVAKDKILFDNLTPL
                     YPQEKLRMEFKAEDMSTRIMDLLTPIGKGQRGLIVSPPRAGKTVLLQKIANAITENHP
                     EVMLIVLLIDERPEEVTDMERSVKGEVVSSTFDEPAERHVQVADMVIEKAKRLVEHNR
                     DVVILLDSITRLARAHNTVVPHSGKILSGGVDANALQKPKRFFGAARNIENGGSLTIM
                     ATALIETGSRMDDVIFEEFKGTGNMEVILDRKLADKRVFPAIDINRSGTRKEELLMTG
                     EELNKVWILRKFLNELNPMEAMEFLIGKMSQTKTNKKFLAMMNT"
     gene            1651..1887
                     /gene="rpmE"
                     /locus_tag="VFR25_08790"
     CDS             1651..1887
                     /gene="rpmE"
                     /locus_tag="VFR25_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET9941189.1"
                     /translation="MKKGIHPGYFNTKIICACGNVIETRATVKEALHVEICSACHPFF
                     TGKQKLVDTAGRIERFRRKYGMKEQTPPAEAPTA"
     gene            1972..2799
                     /locus_tag="VFR25_08795"
     CDS             1972..2799
                     /locus_tag="VFR25_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9941190.1"
                     /translation="MMRSPKRVATAVRLPDGTIQVKTREYVSFSRRFPFLGLPILRGA
                     VVLVESLRIGVEALAFSAEVAVEEDKDKPKSERRYGTLGLGMGFTVLFSFVMAFGIFF
                     YLPLKLTDLTGVRDPLAYNLIDGGIRLVFFMIYIVAIGYWREMQRVFQYHGAEHKTIH
                     NLEAGEDLTPEAADRMSRFHPRCGTSFLFLVVIVSFIVFAFLGRPDTIGARALRFALI
                     PVIAGISFEIIRFAGAHYDRPWVRWLSEPGLLLQRLTTREPSRDMLEVAIAALRAVL"
     gene            2815..3891
                     /gene="prfA"
                     /locus_tag="VFR25_08800"
     CDS             2815..3891
                     /gene="prfA"
                     /locus_tag="VFR25_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9941191.1"
                     /translation="MREALDKAKSRFDDLTNQLADPSVVGNPAELKRVSKERSSLEAL
                     VVAARRYDEVLKQIAENTALVRSEKDAELVAMAKAEVDELEQERAKLEQDLPRLLLPP
                     DPLDHKNVIVEIRAGTGGEEAALFAAEILRLYMKYAERHGWRPELLSTSEAGAGGLKE
                     AVVQINGDGAYSRLKYESGVHRVQRVPETEGSGRIHTSAITVAVLPEAEEIDVEIKPS
                     DLQIDVFRSSGPGGQSVNTADSAVRIRHIPTGIVVQCQDERSQLKNKAKALKVLRARL
                     LDIEIQEQERKLAATRKSMVSSGDRSAKIRTYNFPQGRVTDHRIGLTLYRLQDVLEGD
                     LDELVDGLFNAEQAERLAASGATG"
     gene            3888..4820
                     /gene="prmC"
                     /locus_tag="VFR25_08805"
     CDS             3888..4820
                     /gene="prmC"
                     /locus_tag="VFR25_08805"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9941192.1"
                     /translation="MSAARDAIESGAAPSAAPTTLTRSEALTRARERLIRVRVDAPER
                     EAEWLLLHALGIPPVAFWSEPHAPIAPDREAVLESLIQRREGREPLQLILGEVPFHGV
                     ALAVEPGVFLPRPETEELVEVVLERIAAMRVARGRLLDWGTGSGAIGVALAKALPGWT
                     VVAADWSPIARCLAARNAERNGVEFRVEVVAGDFDAPEAVTSRRTLFDVVVSNPPYVR
                     RGDIPGLMPEVRDHDPREALDGGPDGLDAFRSLARGMHAWLRTGGLLALEIGADQADN
                     VLGILSVPILDARVLPDRAGHPRYVIGTMRGGGA"
     gene            4877..6136
                     /gene="murA"
                     /locus_tag="VFR25_08810"
     CDS             4877..6136
                     /gene="murA"
                     /locus_tag="VFR25_08810"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9941193.1"
                     /translation="MDEIRVLGGYKLKGRVAAGGSKNATLPLLAATLLAPGTYRFTNV
                     PELKDVSTMLEMLSRFGITSRRSGDHELTVDSTNSHNAEAPYELVKTMRASIYVLGPL
                     LARYGEARVSLPGGCAWGPRPVDLHIRAMEQLGAKVEIDHGYIVATAPDGLRGGSISF
                     DVSSVGATGNLLLAAATAAGRTVVENAACEPEIQALCEFLVAMGAKIEGICRPQLIID
                     GVPELHPADAPMIPDRIEIGTLLVAAAITGGDVEVTGVDPTHLGATLSKLEATGASIE
                     VAPDSVRIRAPERPEPISVTTRPFPGFPTDMQAQFMALLSVGNGASTIVDTIYHDRFT
                     HVAELQRLGADIRLDGNTAHVHGVPSLSGAQVMATDLRASAALILAGLIASGETRVSR
                     VYHLDRGYERLEQKLQALGASIRRVKA"
     gene            6158..8047
                     /gene="selB"
                     /locus_tag="VFR25_08815"
     CDS             6158..8047
                     /gene="selB"
                     /locus_tag="VFR25_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET9941194.1"
                     /translation="MRPVVIGTAGHIDHGKTALVRRLSGIDTDRLKEEKERGISIDLG
                     FAHLTLPSGRKVALVDVPGHERFVKNMLAGATGIDLVLLVIAADEGVMPQTREHLAIV
                     DLLGVGDGVITLTKKDLVAPEMLRLVEEEARALVEPTALRSAPIIAVSSTTGDGVEAL
                     LAELDRAVTRVEGRDASGPARLPIDRVFSVEGIGTVVTGTLWSGTVHPGDTLVLLPGG
                     QAVRARQVEVHDQATQAATAGNRTAIALHGISREDVARGDWLAAPGRFHTTEILDARV
                     TLLESARVLANRSRVRVHLGASETLARVVLLDRDELLPGASALAQLRLEGPVVAVPGD
                     RLVLRAYSPSVTIAGARVIDANPPRRARLGDGAREALETLDAGGLEERVALLVRQASF
                     AGARVDVVALRLGAEPAAVEAAASSLLRLNDGRWLDRAAWTALLARITDAVRRYGEAH
                     KLRHGIPKGELKSLLAREIEGTVFDEALDRLLADRTLRADQDKIALPDVGPTFAPEQV
                     RALEAIERRLDAAGFQVPDMAQLLREVPASARPPELARYLVESGRAVRVTSDLYYPAA
                     RWAEVEARVRGHFSQKSALTMGEFKDLIQVSRKYAVPLLEHLDRLGITRREGDLRVPG
                     PRVRT"
     gene            8274..8585
                     /locus_tag="VFR25_08820"
     CDS             8274..8585
                     /locus_tag="VFR25_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941195.1"
                     /translation="MGHLSGTRGTKKLSSTLLALIALSALLATWAPVPSISVGAAPAW
                     ASGSPDETLNPHNGPKSAPAPTTTYVASTKSATTPITTSNITWTSYLLMAWKYLAVSR
                     F"
     gene            8629..11091
                     /locus_tag="VFR25_08825"
     CDS             8629..11091
                     /locus_tag="VFR25_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9941196.1"
                     /translation="MDKTTAELIREREEAVSTARLTQDPERHLQSLEDLAEIFLRADS
                     YLPALQNLEECIQAAERLGLDPHRIGTLELKAAQALIEKGDAPGALKHLARARARAVP
                     DTQPELIARLQLQSARALIELSQYEEALGSCERAHVYFKEKNLMAPLAHAYNCFGRIH
                     FRLGDLDKAKEFYEAALHLFRWDLNDEEGVIRAHNNLGVLYRHLSDWRQSTWHLLRSM
                     ELSTRLGNFAYIAVSCANLGIVFLKAGSWDEAVEHFDRAMNSYVQIGKEAGVARMRIG
                     LATVATLRHDFTAAEGHLAAAVQITSRLGCSREQVLCLFSRGELMVEMSRVEEGLGLY
                     DEALAMARQIAPEGDMVHEIQRRRAEAFALRGDIPAALKAAEDALQRSCKLKDQLEEG
                     GARRVLAAIHTLEGRMSEANTQGREAIRVLESIHERFELARAYLEQARRMPGGDVERT
                     RDAQNLAFKAMYLFEQLGIEECMRSCEHALRDLNAPVWLTAQRAPRVAVPSSSQAQDI
                     ARAYGVLSQNPRTLELVRKASELVTTPARVLIQGETGTGKNLLAYMFRSYEIERGRPF
                     VEVNCTSLPGDLVESELFGYVRGAFTGAAITKRGIFEEADGGTIFLNEIGELGERTQV
                     KLLQVLDDGTYRRLGEVRSRRLNVRIISATNKDLDSAVKAGWFRADLFYRLGQVVLSL
                     PPLRERREDVPLLIRNFFDELSAREGRHVVLAEDAMEYLVSLPWMGNIRELKHKIESI
                     YLCAGRQELVDRATLVRLLYPGGTPAAEAAPVRGLRGKVDLMKREEILAALSRNGGNR
                     SRAALELGITRRHLIRLLKQIY"
BASE COUNT         1920 a         3963 c         3749 g         1761 t
ORIGIN      
        1 tcattgattc cggggatctc ggacgcgctc cggcgcgtcc gagattgacc ttgacatagg
       61 gtcctgatgg ccgatagatt catcggcagg accaccccgg gggcacgata gcgccccgcc
      121 gcacccagta ccggatcgtc aacgcgatcc cgagcccggc cgccgccagg ctctcattcc
      181 ttcgaagaac cgatccccca agatcttgag ggccttcgtt ccttaggtca cgtttatgtc
      241 ccgcccacca acccaagtca ggcgccgcgc cgcggatgcg tccgccgcgc cgaccacaat
      301 cgaggagtca caaagagaaa tggagcagca aaccgaaagc ccgcctgccg tggcgacagg
      361 ctcccctgcc tcgcccaaag ggctcgacat catccagctc aaaggccgca cgattgcgga
      421 gctgatggag ctggcgcact cgctcggcat ccaaggtacg agcgggctca gaaaacagga
      481 gctgatcttc aagatcctgg aaggccagac cgagaagaac ggcctgatct tcgccgaggg
      541 ggtcctggag atcctttccg aggggtatgg cttcttgcgt tcccccgact acaactacct
      601 gccggggccg gacgacatct atgtctcgcc ctcgcagatc aagcggttcg acctgcggac
      661 cggggacacg gtctccggcc aggtgcgccc gccgaaggac ggggagcgat acttcgccct
      721 ccttcgggtc gaggcggtca atttcgagag cccggaggtc gcgaaggaca agatcctctt
      781 cgacaacctg acgcctctct acccgcagga aaagctgcgg atggagttca aggcggagga
      841 catgtcgacc cggatcatgg atctcctgac gcccatcggg aaggggcagc gcggcctcat
      901 cgtgtcgccg ccccgcgcgg gaaagacggt gctcctccag aagatcgcga atgcgatcac
      961 ggagaaccac cccgaggtga tgctcatcgt gctcctcatc gacgagcgcc ccgaggaggt
     1021 caccgacatg gagcggtccg tgaagggtga ggtcgtctcg tccacgttcg acgagccggc
     1081 ggagcgccac gtgcaggttg cggacatggt catcgagaag gcgaagcgtc tcgtggagca
     1141 caaccgggac gtcgtgatcc tcctcgactc gatcacgcgc ctcgcccgcg cccacaacac
     1201 ggtcgtgccc cactcgggca agatcctgtc gggcggcgtg gacgcgaacg cgctccagaa
     1261 gccgaagcga ttcttcggag ccgctcgcaa catcgaaaac ggcgggagcc tcacgatcat
     1321 ggccacggcc ctgatcgaga ccggcagccg catggacgac gtgatcttcg aggagttcaa
     1381 gggcacgggc aacatggagg tcatcctcga ccgcaagctc gccgacaagc gcgtgttccc
     1441 ggccatcgac atcaaccggt cgggcacccg caaggaagag ctgctcatga cgggcgagga
     1501 gctgaacaag gtctggatcc tccgcaagtt cttgaacgag ctgaacccga tggaggcgat
     1561 ggaattcctc atcggaaaaa tgagtcaaac gaagacgaac aagaagttcc tcgcgatgat
     1621 gaacacctga gaggagaacg gagcaatcca atgaagaagg gaatccaccc cggctacttc
     1681 aacaccaaga tcatctgcgc gtgcggcaac gtcatcgaga cgcgcgcgac ggtcaaggaa
     1741 gctctgcacg tcgaaatctg ctcggcttgc cacccgttct tcacgggcaa gcagaagctc
     1801 gtcgacacgg ccggccgcat cgagcggttc cgccggaagt acgggatgaa ggagcagacc
     1861 ccgccggccg aggctcccac ggcctagcgg ccgcacccgg atcccagcca acccatccgg
     1921 atgtcggacc gctacgcccc gatcgggggc caggccgtca tcgagggcgt catgatgcgg
     1981 tcgccgaagc gcgtggcgac cgccgtgcgc cttccggacg gcaccatcca ggtgaagacg
     2041 cgcgagtacg tctcgttctc gcgccgcttc ccctttctcg gtcttcccat cctgcgcggc
     2101 gccgtggtcc tcgtggagtc gctccggatc ggcgtcgagg ccctcgcctt ctcggcggag
     2161 gtcgcggtcg aggaggacaa ggacaagccg aagtccgagc gtcgctacgg caccctcggt
     2221 ctcggcatgg gattcacggt cctcttctcc ttcgtcatgg cgttcggcat cttcttctat
     2281 ctcccgctca agctgaccga cctgaccggc gtgcgcgatc cgctcgccta caacttgatc
     2341 gacggcggga tccggctcgt cttcttcatg atctacatcg tggcgatcgg ctactggagg
     2401 gagatgcagc gcgtcttcca gtatcacggc gccgagcaca agacgatcca caacctggaa
     2461 gcgggggagg atctgacccc cgaagccgcc gaccggatga gccgcttcca tccgcgctgc
     2521 ggcacgagct tcctcttcct cgtggtcatc gtgagcttca tcgtgttcgc cttcctgggc
     2581 cgtcccgata cgattggcgc gcgcgcgctg cgcttcgccc tcatccccgt gatcgcgggg
     2641 atttccttcg agatcatccg cttcgcgggc gctcactacg atcgcccgtg ggtgcgctgg
     2701 ctgagcgagc cgggactcct cctccagcgc ctcaccacgc gcgagccgag ccgcgacatg
     2761 ctcgaggtcg cgatcgccgc gctccgcgcg gtcctctaac caggcccgtc ccccatgcgt
     2821 gaagccctgg acaaggccaa gtcccgtttc gacgacctga cgaaccagct cgccgatccc
     2881 tcggtggtcg ggaacccggc cgagctgaag cgcgtgagca aggagcggag ctccctcgag
     2941 gcgctcgtcg tagcggcgcg gcgctatgac gaggtgctga agcagatcgc ggagaacacg
     3001 gcgctcgtcc ggagcgagaa ggacgccgag ctcgtcgcga tggcgaaggc cgaagtggac
     3061 gagctcgagc aggagcgtgc gaagctcgag caggacctgc cacggcttct cctgccgccc
     3121 gatccgctcg atcacaagaa cgtgatcgtg gaaatccggg ccggcaccgg cggggaagag
     3181 gccgcgctct tcgcggccga gatcctgagg ctctacatga agtacgccga gcgccacggg
     3241 tggcgccccg agctcctctc gacgagcgag gccggcgcgg ggggcttgaa ggaagccgtg
     3301 gtccagatca acggcgacgg cgcctacagc cggctcaaat acgagagcgg cgtccatcgc
     3361 gtgcagcgcg tccccgagac cgaagggagc gggcgcatcc acacctcggc catcacggtc
     3421 gcggtgctcc ccgaggcgga ggagatcgac gtggagatca agccgtccga tctccagatc
     3481 gacgtcttcc gctcgtcggg tcccggcggc cagagcgtca acaccgccga ctccgccgtc
     3541 cgcatccggc acatcccgac cggcatcgtg gtccagtgcc aggacgagcg ctcgcagctc
     3601 aagaacaagg cgaaggcgct caaggtgctc cgcgcgcgcc tcctcgacat cgagatccag
     3661 gagcaggagc ggaagctcgc cgcgacccgg aagtcgatgg tgtcgagcgg ggaccgcagc
     3721 gcgaagatcc gcacctacaa ttttccgcag gggcgcgtca ccgatcacag gatcgggctc
     3781 acgctctacc ggctgcagga cgtgctcgaa ggggacctgg acgagctcgt ggacgggctg
     3841 ttcaacgccg agcaggcgga gcggctggcc gcctcggggg ctacggggtg agcgcggcgc
     3901 gcgacgcgat cgagtccggc gcggctccga gtgcggctcc aacaaccctc acccgcagcg
     3961 aggcgctcac ccgcgcgcgc gagcggctga tccgggtccg agtcgacgct cccgagcgcg
     4021 aggcggagtg gctcctcctc cacgcgctcg gcattccccc ggtcgccttc tggtccgagc
     4081 ctcacgcccc gatcgccccg gaccgtgaag cggtcctcga atccctgatc cagagacgcg
     4141 aaggacgcga gccgctccag ctcatcctgg gcgaggtccc gttccacggc gtcgcgctcg
     4201 cggtcgagcc cggcgtgttc ctgccgcgcc cggagaccga ggagctggtg gaggtcgttc
     4261 tcgagcggat cgcagccatg agagtggccc gcgggcgtct tctcgattgg ggaaccggct
     4321 cgggcgccat cggggtcgcg ctcgcgaaag cccttcccgg ctggaccgtg gtggcggccg
     4381 actggtcccc catcgcccga tgtctcgccg cccggaacgc cgagcggaac ggcgtggaat
     4441 ttcgggtgga ggtggtggcc ggcgatttcg acgcccccga ggcggtcacg agccgccgca
     4501 ccctgttcga cgtggtcgta tccaaccccc cctacgtccg gcgcggggac atccccgggc
     4561 tcatgcccga ggttcgcgac cacgatcccc gcgaggcgct tgacggcgga ccggacggac
     4621 tcgatgcttt tcgctcactg gcccggggaa tgcatgcgtg gctccgtacc ggtgggctcc
     4681 tcgcgctgga gatcggcgcc gatcaggccg ataacgtcct ggggatcctt tcggttccca
     4741 tcctggacgc gcgggtcctg cccgatcggg cggggcatcc gcgatacgtg atcggcacca
     4801 tgcgaggcgg gggagcatga agatcgagca ggcacgcgcg cgcagcacga acgacaccga
     4861 ggtcaccact accggcatgg atgaaatccg agtcctgggc ggctacaagc tgaagggccg
     4921 agtcgccgcg gggggatcca agaacgcgac cttgccgctc ctggcggcga cgctcctcgc
     4981 tcccggcacc taccgcttca ccaacgtgcc cgagctcaag gacgtctcca ccatgctgga
     5041 gatgctctcc cggttcggca tcacgagccg ccgctcgggc gatcacgagc tcacggtcga
     5101 ctcgacgaac tcacacaacg ccgaggcgcc gtacgagctg gtgaagacca tgcgcgcctc
     5161 gatctacgtc ctgggtccgc tcctcgcgcg ctacggcgag gcgcgcgtgt cgctccccgg
     5221 cggctgcgcg tggggaccgc gtcccgtcga tctccacatc cgggcgatgg agcagctcgg
     5281 cgccaaggtc gagatcgatc acggctacat cgtcgcgacc gcgccggacg ggctccgcgg
     5341 cggcagcatc tcgttcgacg tctcgagcgt cggcgcgacg gggaacctcc tcctggccgc
     5401 ggccaccgcg gcgggacgca ccgtcgtcga gaacgccgcg tgcgagcccg agatccaggc
     5461 gctctgcgag ttcctcgtcg ccatgggcgc gaagatcgaa ggaatctgcc ggccgcagct
     5521 catcatcgac ggcgtgccgg agctccatcc cgcggacgcg ccgatgatcc cggaccggat
     5581 cgagatcggt acgctcctcg tggcggccgc gatcacgggc ggcgacgtcg aggtgaccgg
     5641 tgtggatccg acccatctgg gcgcgaccct ttccaagctc gaagccacgg gcgcgtccat
     5701 cgaagtcgct cccgattccg tacgcatccg tgcaccggag cgtccggaac cgatctccgt
     5761 cacgacccgt ccgttccccg ggttcccgac ggacatgcag gctcagttca tggcgctcct
     5821 ctcggtgggc aatggcgcca gcacgatcgt cgacaccatc taccacgacc gcttcacgca
     5881 tgtggccgag ctccagcgac tcggggcgga catccgcctc gacggcaaca cggcccacgt
     5941 gcacggcgtg ccctccttga gcggcgcgca ggtcatggcc accgatctcc gtgcgtccgc
     6001 ggctctgatc ctggccgggc tcatcgcgag cggggagacg agggtgtcgc gcgtgtacca
     6061 cctcgaccgt ggctacgagc ggctcgagca gaagctccag gcgctcgggg cgtcgatccg
     6121 ccgcgtcaag gcctgacggc cacccgctag cgaacgaatg cgccccgtcg tcatcggcac
     6181 cgcggggcac atcgaccacg gcaagaccgc gctcgtccgc cgcctctcgg gcatcgatac
     6241 cgatcgcctg aaggaggaga aggagcgcgg catctccatc gacctcggct tcgcgcacct
     6301 gacgctcccc tcggggcgga aggtcgcgct cgtcgacgtg ccggggcacg agcggttcgt
     6361 gaagaacatg ctcgccggcg cgaccggaat cgatctcgtg ctcctcgtga tcgcggcgga
     6421 cgagggcgtg atgccgcaga cgcgcgagca cttggcgatc gtggatctcc tcggcgtggg
     6481 cgacggcgtg atcacgctca cgaagaaaga tctcgtcgcg ccggaaatgc tgcggctggt
     6541 cgaggaggaa gcgcgggcgc tggtcgagcc gaccgcgctc cgctccgcac ccatcatcgc
     6601 ggtctcctcg accaccggcg acggcgtcga agcgctcctc gccgagctgg atcgcgcggt
     6661 cacgcgcgtg gagggacgcg atgcgtccgg ccctgcgcgg cttcccatcg atcgcgtctt
     6721 ctcggtcgaa ggcatcggca ccgtcgtgac cgggacgctc tggagcggca ccgtgcatcc
     6781 cggcgacacg ctcgtcctcc tgcccggcgg ccaggccgtt cgtgcgcgtc aggtcgaggt
     6841 gcacgaccag gccacgcaag ccgccaccgc cggaaaccgg accgcgatcg cgcttcacgg
     6901 catctcgcgc gaggacgtcg cgcgcggcga ctggctggcc gcgccggggc gcttccacac
     6961 gacggagatc ctggacgcgc gtgtgacgct cctcgagtcg gcgcgcgtgc tcgcgaaccg
     7021 atcgcgcgtg cgcgttcatc tcggggcgag cgagacgctc gcccgcgtcg tgctcctgga
     7081 ccgggacgag ctcctccccg gcgcgtcggc gctcgcgcag ctcaggctcg aagggcccgt
     7141 cgtggcagtg cccggagacc ggctcgtgct ccgcgcctac tcgccatcgg tcacgatcgc
     7201 gggagcccgt gtgatcgacg ccaatccgcc gcggcgcgcg cggcttggcg atggcgcgag
     7261 ggaggcgctc gagacgctcg atgcaggcgg tctcgaggag cgcgtggccc tcctcgttcg
     7321 gcaggcgagc ttcgccggtg cgcgcgtcga cgtcgtggcg ctccggctgg gcgcggagcc
     7381 cgcggccgtc gaagcggccg cgtcgtcgct ccttcgactg aacgacggtc gctggctcga
     7441 tcgggcggct tggacggcgc tcctcgcgcg gatcacggac gcggtgcgcc gctacggcga
     7501 ggcgcacaag ctcaggcatg ggatcccgaa aggggagctg aagagcctcc tcgcgcgcga
     7561 gatcgagggg actgtcttcg acgaggcgct ggaccgcctt ctcgccgacc gcaccctgcg
     7621 tgccgatcag gacaagatcg ctctccccga cgtcggcccg acctttgcgc ccgagcaggt
     7681 ccgggcgctc gaggccatcg agcgccgcct ggacgccgcg ggctttcagg tgcccgacat
     7741 ggcccagctc ctccgcgagg tgccggcctc cgccaggccc cccgagctgg cccgatacct
     7801 ggtcgagtcc ggacgtgccg tccgcgtcac atcggatctc tactacccgg ccgcccgctg
     7861 ggccgaggtc gaggcccggg tccggggcca cttcagccag aaatcggccc tgaccatggg
     7921 ggagttcaag gatctgattc aggtttcccg gaaatatgcc gttccattgc tggaacacct
     7981 tgaccgtctg gggattacaa ggcgggaagg ggatctcagg gtgccgggcc cccgcgttag
     8041 gacttagaag gggatggact cggccccagc cggagggccg ccccctgaat cggcgcctcc
     8101 aataccccga tttcgggtga gacttgggtg tcccaccgaa aagaattgct ggcgaattcg
     8161 gcgtaggggt atcatctttg ttaatagatc gcccggccgt ccctccggtc ggctacctgc
     8221 ggagatccct cagggggatt tccgggatca accacaaagt aggtgaagcc gccatgggcc
     8281 atctctccgg aacccgcggg accaagaagc tctcgtcgac cctgctcgcg ctcatcgcac
     8341 tgtccgctct gctagcgacg tgggctccgg tgccctcgat ttccgttgga gccgcgcccg
     8401 catgggcgag cggctcgccg gacgagacac tgaatccaca caacggcccg aagagcgcgc
     8461 cggcaccgac cacgacgtac gttgcgtcca cgaagagcgc gacgacgccg atcaccacgt
     8521 cgaatatcac ctggacgagc tacctgctga tggcgtggaa gtacctggcc gtttcgcgat
     8581 tctgaacaga ggggccgcct atggccccat ctcatcggaa gacggtgcgt ggacaagacc
     8641 acggcggaat tgatccggga gcgtgaggag gcggtatcca ccgcccgcct cacgcaagat
     8701 ccggaacgac atctccaatc cctcgaagat ctcgcggaga tcttcctcag agccgatagc
     8761 tatctccccg ccctccagaa tctcgaggag tgcatccagg cggcggagcg gctcggcctc
     8821 gatccgcatc gcatcggcac gctcgagctg aaggcggcgc aggccctgat cgagaagggg
     8881 gacgcgcccg gcgcgctcaa gcatctggcg cgtgcgcggg ctcgcgcggt tcccgacacg
     8941 cagcccgagc tgatcgcgcg gctccagctc cagagcgcgc gggccctgat cgagctgtcg
     9001 cagtacgaag aggcgctggg aagctgcgag cgcgcccacg tctacttcaa agagaagaac
     9061 ctcatggcgc cgctcgcgca cgcgtacaac tgcttcggcc ggatccattt ccgcctcggc
     9121 gacctggaca aggcgaagga gttctacgag gccgcgctcc acctcttccg ctgggatctg
     9181 aacgacgagg aaggggtcat ccgcgcccac aacaacctgg gcgtcctcta ccgtcacctc
     9241 tccgattggc gccagtcgac ctggcatctg ctccgctcga tggagctgtc gacccgcctc
     9301 ggtaatttcg cctacatcgc ggtctcctgc gccaacctcg gcatcgtctt cctcaaagcc
     9361 ggatcctggg acgaagcggt cgagcacttc gaccgcgcca tgaactccta cgtccagatc
     9421 ggcaaggaag cgggcgtcgc gcgcatgcgg atcgggctcg ccacggtcgc cacgctgcgg
     9481 cacgacttca ccgccgccga agggcacctg gcggccgcgg tgcagatcac ctcgcgcctc
     9541 ggctgctcgc gcgagcaggt gctctgcctg ttcagccgcg gcgagctgat ggtggagatg
     9601 agccgcgtcg aggagggtct cggactctac gacgaggcgc tcgccatggc gcgccagatc
     9661 gcgcccgaag gggacatggt tcacgagatc cagcgccgcc gcgccgaggc cttcgcgctc
     9721 cggggcgata ttcccgccgc gctcaaggcc gcggaagacg cgctccagcg ctcctgcaag
     9781 ctcaaggatc agctcgagga aggcggcgcg cgccgcgtgc tcgccgcgat ccacacgctc
     9841 gaagggcgca tgtccgaggc gaacacgcag ggtcgcgagg cgatccgagt cctcgaatcc
     9901 atccacgagc ggttcgagct cgcccgcgcc tatctcgagc aggcccgccg catgccgggg
     9961 ggcgacgtcg agcgcacgcg tgacgcgcag aatctcgcgt tcaaggcgat gtacctgttc
    10021 gagcagctcg ggatcgagga gtgcatgcgc tcctgcgagc acgcgctgcg ggatctcaac
    10081 gcccctgtct ggctgacggc ccagcgcgcg ccgcgcgtgg cggtgccgtc ctcctcgcag
    10141 gcccaggaca tcgcccgtgc ctacggtgtc ttgagccaga acccccgtac gctcgagctg
    10201 gtgcggaagg cctcggagct cgtcaccacg ccggcgcgcg tcctcatcca gggcgagacc
    10261 ggcacgggaa agaacctgct cgcctacatg ttccgctcct atgaaatcga acgcggtcgt
    10321 ccgttcgtcg aggtgaactg caccagcctc ccgggcgatc tcgtcgagag cgagctcttc
    10381 ggctacgtgc ggggcgcctt cacgggcgcc gcgatcacca agcgcggcat cttcgaggag
    10441 gcggacggcg ggacgatttt cctgaacgag atcggggagc tgggcgagcg cacgcaggtg
    10501 aagctcttac aggtcctgga tgacgggacc taccgccgcc ttggcgaggt gcgctcgcgc
    10561 cggctcaatg tccggatcat ctcggccacg aacaaggatc tcgactccgc cgtgaaggca
    10621 ggctggttcc gggcggatct cttctaccgt ctcgggcagg tcgttctctc gttgccgccg
    10681 cttcgcgagc ggcgcgagga cgtcccgctc ctgatccgga acttcttcga cgagctgtcg
    10741 gcccgagagg gacgccacgt cgtgctcgcc gaggacgcga tggagtatct cgtgtcgctc
    10801 ccgtggatgg ggaacatccg cgagctcaag cacaagatcg agtcgatcta cctctgcgcg
    10861 ggacgccagg agctggtgga tcgcgccacg ctcgtccggc tcctctatcc gggcggcacc
    10921 ccggcggccg aggccgcccc ggtgcgcgga ctgaggggca aggtcgacct catgaaacgc
    10981 gaggagatcc tcgcggcgct ctcgcggaac ggcggcaacc ggagccgcgc cgcgctcgag
    11041 ctcggcatca cccgccggca cctcatccgg ctcctcaagc agatctatta agcgcggcgc
    11101 cgcagcgccg cggaactcca gctagatctc cgcttcggaa ccctacccac cacggctggt
    11161 tatcatccat gagcccgatg gcccagaccg cgcgcggcct tcaggtctgt gacatccacg
    11221 tccctttccc ctgtcggggc gtgacatcgc tcccaagatc tccgcgggga agcgattaag
    11281 cgctctcagt gatgcgaggt ctgtgacatc gatgtcccag gacccccacc ttgacaggct
    11341 tccagcctgc tagcgccggt tcgtgtcgaa ccttcaagcc cttcccgcgc aac
//
LOCUS       DASTKC010000213        10346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1357535, whole genome shotgun sequence.
ACCESSION   DASTKC010000213 DASTKC010000000
VERSION     DASTKC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357535"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFR25_08830"
     CDS             <1..432
                     /locus_tag="VFR25_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941197.1"
                     /translation="TLAFAPDEARARFWSQWNEMTYLGERADSAAVARGFQSLLDGSR
                     DLAPDSLELLARYVLQTTEDQPGREIAHEERRTIHGAEWIVVDTWSAVSHMDRRRIAC
                     LDDGGYLLALRTKRGTVERAGLVFEGLLQSIVAVADTTRAL"
     gene            complement(429..1541)
                     /locus_tag="VFR25_08835"
     CDS             complement(429..1541)
                     /locus_tag="VFR25_08835"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HET9941198.1"
                     /translation="MSRVWGALALALLALSGCARQAPRQLWEATTIPTDAQFDGMWFT
                     DSLNGWITGGGYAIDGGIVGRTRDGGRTWSFQSGVLRGGGERFSMGGVQFLDSLRGVS
                     VGYGGIVLVTSDGGANWRPVRYGRTGGDGLSEVQFIDSRNGWAVGSASVVRTHDGGET
                     WSLLVTNNAENGYLSGNAIHFVDLWNGWMVGHSGLLYRSSDGGENWTRVPLPLREDEH
                     PTFWDVTFTDAAHGWVVGEQGTIFHTEDGGVAWVREDNGVPIVRVLPPGESPRHEVVP
                     ELETEPDRLSISSVQFADNANGFAVGYYADVAESVVLATSDGGATWRVERVVPGELLR
                     SLFLLDAHHAWAAGDRARMTPQVVLRRLTDGGSSSG"
     gene            complement(1538..2077)
                     /locus_tag="VFR25_08840"
     CDS             complement(1538..2077)
                     /locus_tag="VFR25_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941199.1"
                     /translation="MNATLLRTWRCGLAVAALAAMITTPGLVSGSDDAAKKANLGEVL
                     ERAESMPSDARRDTDKRIAITVERVNQEARDRGQATMTARLAAEFKVTGESLLEDKSE
                     YSLSWGELVIAHTLLANSGAGLGLVDLVDLRREGLGWGAIAFGLEFHMEDFEDMIKAE
                     GRVAMGLSKTGGNVPPNLK"
     gene            complement(2091..3296)
                     /locus_tag="VFR25_08845"
     CDS             complement(2091..3296)
                     /locus_tag="VFR25_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941200.1"
                     /translation="MIRRPASAILITLALLAPPARAGDTASKKAVPRSTATAVPITPS
                     SPVTWGREAAPYHGSLDNITWGGHGELRFGYDVTAGVSDPSWFSVGRVNGFMEAKLSS
                     KVRIRGQAAYDHVPDDFTLERAELMASLGRSHEAHAGIFLVPLGRTNLSHDAPAYEFW
                     ERSLVATQILGVPNAQLGLGVSGTGSWSRTTPLRYEIDLVTGFDDGVIMDASGGTRIP
                     AGRNNYSDQNGIPAAVARFAIQPGRGEVGVAIQSGPYNRTHVEGAEVDGTRWVHVLTA
                     DGIAHAMGLELATEAAAALIDIPPGLEGLYAERQWGASLEATRTLRAPLFHAWRGTSL
                     SGGLRAETVDFDAGLAGDSRTRVAASLNIRPQPTSILRFGWYYEWKRDRFDNLTPLAG
                     LTLTAASYF"
     gene            complement(3293..4519)
                     /locus_tag="VFR25_08850"
     CDS             complement(3293..4519)
                     /locus_tag="VFR25_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET9941201.1"
                     /translation="MSHSWRSQSRIAFCAAGLVLAALVIVGCSNLLTTEPPAGERFDQ
                     PLAGLSNGELGDFQDGHTQFRKAFTINEGLGPIFNNVSCAACHSGDGRGVTGNTLTRF
                     SRGTDLDYAEGGPQLQDKAVPGATPERLPSGVDVSYRLPPPVFGVGLIEAIPDSAILA
                     GEDPGDANADGISGRANWVTPASYLPSDDPGAGPSRVGRFGRKAANSSLIQQVTEAYH
                     QDMGITSPYRPTENVNPAAPAASGADPAPEPEVSEAKISDIAQYMRMLAPPSPGEMSP
                     ARERGQTLFAGAHCTGCHTPVMHTGWHEIEALRNRDAQLYSDLLLHDMGDALADNRPD
                     GSADGHEWRTAPLWGLRIAREFLGGDLLLLHDGRAHSVDEAVRLHGGEAAAARDAYIA
                     MPVDDKAALVDFVESR"
     gene            4679..5302
                     /locus_tag="VFR25_08855"
     CDS             4679..5302
                     /locus_tag="VFR25_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941202.1"
                     /translation="MKALSMIVAWIVAVVLIVPGSALAGTKAQADVDNTHHSSGPGFL
                     VSAGAVEFSPTVSFTHQNFKREGYGNVDHFTQLDVRPTVGFCVSDHYEVMAGFLARHT
                     SVNGSDDTALGATAGLTYNFSRHGSVIPFVGLGFGALFYDGFTMDNTAVLAPMLSGGV
                     RVPVGGMAAVNMSVGYQHESNADGEFNASANRIVGSVGVSLFPWRTH"
     gene            5390..5857
                     /locus_tag="VFR25_08860"
     CDS             5390..5857
                     /locus_tag="VFR25_08860"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HET9941203.1"
                     /translation="MNYRVRGLSPDPFEPLFQMSDDELAERGAVRRVADEPHAYPCRV
                     SLAEAEPGEELLLLPYRHQTSASPYQASGPIYVRRNARASFDALNRIPAMQERRLSSV
                     RGYDAAGFMQECDVVAGTDLDATIRRFFANPEVYTIHVHNARPGCFAFRVERA"
     gene            5854..6429
                     /locus_tag="VFR25_08865"
     CDS             5854..6429
                     /locus_tag="VFR25_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941204.1"
                     /translation="MRRAFLVVAVLALSAASSHAATSERSSAPDPGLTSFRQWLDANR
                     PGYGCDEGPAPFRNKTVEAAYPGQRFYYVLTYTRGIQPPFENSVSLVAAVDDSGRVAP
                     FLPGRPASYSRGLKRIQSSKDARLAGAAVLILGSCGERRWPIAPERVKAKKNSKGWTA
                     TYFYDSYNSSWVSFDKKGAVLAFGGSAPPVP"
     gene            6480..9047
                     /locus_tag="VFR25_08870"
     CDS             6480..9047
                     /locus_tag="VFR25_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941205.1"
                     /translation="MSISAGATLGPYEILAPLGSGGMGDVYRARDPRLGRDVAIKVLP
                     PAFAADPNRLARFEQEARAVAALNHPNIVAIYDVGKDAEGRPYLVTELIEGTTLRTRL
                     LDEPLPLRQAVDFGLQIAHGLAAAHDRGIVHRDLKPENLFVTKDRRVKILDFGLAKVV
                     GPRESRSPESTIAVGATESGTVLGTVGYMAPEQVRGDAADHRSDLFAFGCILYEMISG
                     RRAFHALTAAETMTAILRSDPQPMGAPEQVPSSIERVVRHCLEKAPSERYQSAHDLAF
                     QLQGALDPSDGVAPARPRGRWLAVTALLASIVGGLLAVAPRWLSPPAKPPEFHRITFR
                     PGFAWSGRFAPDGRTIVYSAAWGEAPLETYSTRPESPESRQLGLKNASILSISRSGEM
                     AVLLNASYAGGWAYRGTLARVPLEGGAPREVLDSVLWADWEPGGRDLAVVRESGGERR
                     LEYPIGKVLYKAAGTIGEPRFSPRGDLIAFLDHPIFGDDRGSVSVVDRAGKVRRLTSV
                     WKSIQGIAWAPGGKEIWFTAADEGPIRALYAVTPSGRLRTVMNPPGSLSLQDISPSGG
                     VLLAEWRIRYGITGRSGSDAGERDLSWLDWSAVRDFSHDGTQILFSEGGEGGGPSYGV
                     YLRPTDGSPAVRLGEGETLALSPDGQWVLSVPRGDKPHPVLLPTGAGQARSLPAGPLR
                     EYQNGTWLKDGRVLLEASEPGHGNRYYVQDVNGGMPQAVTPENVTGFGFGAHTAAPDG
                     RNVIARGPGGSIEMWPLGPGEPVPVSGLAAGDVPIRWADDGRTLYVAHAAAGRRELEL
                     ARVDLPTGRRTSWKDLVPRDAIGATRIGSPMVSPDGSAYAYTYGSHTADLYLVEGLR"
     gene            complement(9051..9713)
                     /locus_tag="VFR25_08875"
     CDS             complement(9051..9713)
                     /locus_tag="VFR25_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9941206.1"
                     /translation="MKATRLAAILAGILVLGAAPATKTAPTPTSALTELKAGNERFAS
                     HHATHPHQTAARRTELAASQHPHAVILACADSRVAPEIVFDKGLGDLFTVRVAGNIAG
                     DPEIASIEYAVEHLHVPLIVVMGHQSCGAVGAAIEGGEAPGHLPALIQAITPSVEKAR
                     SMQGDLSDNAIRLNVEAVVAQLAASHPILAEHIAEGKLKIVGGVYSLETGRVAWLAQN
                     TP"
     gene            complement(9710..10105)
                     /locus_tag="VFR25_08880"
     CDS             complement(9710..10105)
                     /locus_tag="VFR25_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941207.1"
                     /translation="MGEFLRQFWDELIGRADGPLTLRLFLQPTMAAIFAIRSGIKDGR
                     EGRPPYLMTLVRDPEHRGALLREGWKDVGKIFVIAMVLDAIYQIIAIHKIRPLQIVLV
                     PVFLALIPYMILRGPASRLAAARKGASKS"
     gene            complement(10120..>10346)
                     /locus_tag="VFR25_08885"
     CDS             complement(10120..>10346)
                     /locus_tag="VFR25_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941208.1"
                     /translation="PGDVLGIEVDLEKNQLTKLSVASYLKKTDDAVTLDATLGTLEGG
                     ITYPAQIVFDVKSTGIKTVIENSGYKKTGT"
BASE COUNT         1575 a         3558 c         3557 g         1656 t
ORIGIN      
        1 acgctcgcct tcgcgcccga cgaggcgaga gcgaggttct ggagccagtg gaacgagatg
       61 acctacttgg gggagcgcgc ggacagcgcg gccgtggccc gtgggttcca gtcgctcctc
      121 gatggatcgc gggatctcgc gcccgactcg ctcgagctct tggcgaggta cgtgctccag
      181 acgacggagg accagccagg gagggagatc gcgcacgagg agcgaaggac gatccacggc
      241 gcggaatgga tcgtcgtcga cacgtggagc gccgtctccc acatggatcg gagacggatc
      301 gcctgtctcg acgacggagg gtatctgctc gcgctccgca ccaagcgcgg aaccgtggag
      361 cgggcgggac tcgtcttcga aggactgctc cagtcgatcg tggcggtggc cgacacgacc
      421 cgggcgctct agcccgagct cgatcctccg tccgtgaggc gtctcaaaac gacctgcggc
      481 gtcatgcggg cgcgatcccc cgccgcccat gcgtggtgtg cgtcgagcag gaagagggag
      541 cgcaggagct cgcccggcac gacccgctcc acgcgccacg tcgcgccccc gtccgaggtc
      601 gcgagcacga ccgactccgc gacgtccgcg taatatccca ccgcaaagcc gttcgcgttg
      661 tcggcgaact gcaccgagga gatggagagg cggtcgggct cggtctcgag ctccggaacc
      721 acctcatgcc ggggcgactc gccgggcggg aggacgcgca cgatcggaac gccgttgtcc
      781 tcacggaccc acgcgacgcc gccgtcctcc gtgtggaaga tcgtgccctg ttcacccacg
      841 acccagccgt gcgccgcgtc ggtaaaggtg acgtcccaaa acgtggggtg ctcgtcctcc
      901 ctgagcggaa gcggaacccg cgtccagttc tctcccccgt cgctggatcg gtacaagagc
      961 ccgctgtgcc ccaccatcca tccgttccag aggtccacga agtggatcgc gttcccggag
     1021 aggtagccgt tctcggcgtt gttcgtgacc aggaggctcc aggtctctcc gccgtcgtga
     1081 gtgcgaacca cgctggccga gcccacggcc catccgttcc gtgagtcgat gaactggacc
     1141 tccgagaggc cgtccccgcc cgtgcgtccg tagcgaaccg ggcgccagtt cgcgccgccg
     1201 tccgacgtca cgagcacgat gcccccgtaa cccacggaga ctccgcgaag cgagtcgagg
     1261 aactggacgc cgcccatcga gaaccgctcg ccgccgcccc tcaggacgcc gctctggaag
     1321 gaccacgtcc gtccgccgtc ccgggtgcgg ccgacgatgc cgccgtcgat cgcgtagccc
     1381 ccgcccgtga tccagccgtt caaggagtcg gtgaaccaca tgccgtcgaa ctgggcgtcg
     1441 gtgggaatgg tcgtggcttc ccagagctgg cggggcgcct gccgcgcgca gccggacaga
     1501 gccaggaggg cgagggcgag cgccccccac acgcgactca cttgagattg ggcggaacgt
     1561 tgccgccggt cttggatagc cccatcgcga cccgtccttc ggccttgatc atgtcctcga
     1621 agtcttccat gtggaattcg agaccgaacg cgatcgcgcc ccagcccaaa ccttcgcgcc
     1681 ggagatccac gagatccacg agaccgagtc cggcgcccga gttggcgagg agcgtgtgcg
     1741 cgatcacgag ctcgccccag ctcaagctgt actccgactt gtcctcgagg agcgactccc
     1801 ccgtgacctt gaactcggcc gcgagccgtg cggtcatcgt cgcctgtccg cgatcccgag
     1861 cctcctgatt cacccgctcc acggtgatcg cgatccgctt gtcggtatcg cgccgggcgt
     1921 ccgagggcat ggactccgcc cgctcgagca cctcccccag gttggccttc ttggccgcgt
     1981 cgtccgagcc cgataccaag cccggggtcg tgatcatcgc cgcgagcgcg gccacggcaa
     2041 gtccacaccg ccacgttctc aacagggtcg cgttcatcgg ctctcccttt ctagaagtag
     2101 ctcgccgccg tgagcgtgag cccggcgagc ggcgtcaggt tgtcgaaacg gtcccgcttc
     2161 cactcgtaat accagccgaa gcggagaatg gatgtcggtt gggggcggat gttgaggctc
     2221 gcggcgacgc gcgtgcgcga gtctcccgcg agtccggcgt cgaagtcgac ggtctcggct
     2281 cggagcccgc cgctcaggct cgtgccgcgc cacgcgtgga acagcggagc gcggagcgtg
     2341 cgcgtggcct cgagcgacgc gccccactgg cgctcggcgt agagtccttc gagccccggc
     2401 ggaatgtcga tgagcgcggc cgcggcctcg gtcgcgagct cgaggcccat cgcgtgcgcg
     2461 atcccgtcgg ccgtgaggac gtgcacccac cgcgttccat cgacctcggc cccttcgacg
     2521 tgggtccggt tgtacggccc gctctggatc gccacgccga cttcgccccg gcccggctgg
     2581 atcgcgaagc gcgccacggc ggcgggaatg ccgttctggt cgctgtagtt gttccgtccc
     2641 gcgggaatgc gcgtgcctcc ggaggcgtcc atgatcacgc cgtcgtcgaa cccggtcacg
     2701 agatcgatct cgtaccggag cggcgtcgtg cggctccacg aacccgtgcc gctcacccca
     2761 aggccgagct gcgcgttcgg cacgccgagg atctgtgtcg cgaccagaga tcgttcccaa
     2821 aattcgtacg cgggagcgtc gtggctcagg ttggtgcggc cgagcggaac gaggaagatt
     2881 cccgcgtgcg cttcgtgcga gcggccgagg ctcgccatga gctccgcgcg ctccagagtg
     2941 aaatcgtcgg gcacgtggtc gtacgccgcc tgcccgcgaa tcctcacctt ggagctgagc
     3001 ttcgcttcca tgaatccgtt gacccggccc accgagaacc acgacggatc cgagacgccg
     3061 gcggtgacgt cgtacccaaa gcggagctca ccgtgtccac cccacgtgat gttgtcgaga
     3121 ctgccgtgat acggggcggc ctcgcgaccc caggtgaccg gagacgacgg ggtgatcggc
     3181 accgcggtcg cggtcgagcg cgggaccgct ttcttcgagg ccgtgtcacc ggcgcgcgcc
     3241 gggggcgcga gcaacgccag cgtgatcagg attgcactcg cggggcgccg gatcatcgcg
     3301 actccacgaa atcgaccagc gccgccttgt cgtccaccgg catggcgata taggcatcac
     3361 gcgccgccgc cgcttccccg ccgtggagcc gcaccgcctc gtcgacgctg tgcgcccgcc
     3421 cgtcatggag gaggaggaga tcgccgccca ggaactcgcg cgcgatccgc agcccccaga
     3481 gcggagccgt gcgccactcg tggccgtcgg cgcttccgtc cggacggttg tcggcgagcg
     3541 catcccccat gtcgtggagg aggaggtccg agtagagctg ggcgtcccga tttcggagcg
     3601 cctcgatctc gtgccagccg gtgtgcatga ccggagtgtg acaaccggtg cagtgcgcgc
     3661 cggcgaagag cgtctgcccc cgctcccgcg ccggggacat ctcgcccggc gacgggggcg
     3721 cgagcatgcg catgtactgc gcgatgtcgc tgatcttggc ctcactcact tcgggctccg
     3781 gagcgggatc ggcgccggac gcggcgggtg cggcgggatt cacgttctcg gtgggccggt
     3841 acggcgacgt gatgcccatg tcctggtgat aggcctccgt cacctgctgg atcaacgatg
     3901 agttcgccgc cttgcgtccg aagcgcccca cgcgcgacgg tcccgcgcca ggatcgtcgg
     3961 acgggagata ggacgcgggc gtcacccagt tggcgcgtcc ggagatgccg tccgcgttcg
     4021 cgtctcccgg gtcctcgccc gcgaggatcg ccgagtccgg gatggcctcg atgaggccga
     4081 cgccgaacac gggaggcggc aggcgatagg acacgtcgac ccccgacgga agccgttccg
     4141 gcgtcgcgcc gggcaccgcc ttgtcttgaa gctgcggccc gccctcggcg tagtcgagat
     4201 ccgtgccgcg gctgaagcgc gtgagcgtgt tccccgtgac gccgcgtccg tcaccggagt
     4261 ggcacgccgc gcaggacacg ttgttgaaga tggggccgag cccctcgttg atcgtgaacg
     4321 ccttccggaa ctgggtgtgt ccgtcctgga agtcgcccag ctcgccgttc gagagtccgg
     4381 cgagcggttg gtcgaagcgc tctccggccg ggggctcggt cgtgaggaga ttgctgcacc
     4441 ccacgatcac gagcgccgcc agcacgagac cggccgcgca gaatgcaatg cgagattgcg
     4501 atcgccacga atgcgacatg ggaacgccgt gcctccccca ggctttaacg gtggtgattg
     4561 gccgagtcgc aagagccgcg ccagcacttg cggggatgag agccatgagg gtccggaact
     4621 tgcgtccgtg aagggccgag cccggcgtgt gcccgcggtt cagggggagg tcctgacgat
     4681 gaaagcgctc tcgatgatcg ttgcttggat cgtagccgtc gtgttgatcg taccggggtc
     4741 ggctctcgcg ggaacgaagg cccaggcgga cgtggacaac acccaccatt cttccggtcc
     4801 cggattcctc gtgtccgcgg gcgcggtcga gttcagcccc accgtctcct tcacgcacca
     4861 gaacttcaag cgggaggggt acggcaacgt ggatcacttc acccagctcg acgtgaggcc
     4921 gacggtcggc ttctgcgtca gcgaccacta cgaagtcatg gccggattcc tggcgcgcca
     4981 cacgtcggtc aacggcagcg acgacaccgc gctcggcgcg acggccgggc tcacgtacaa
     5041 cttctcgcgc cacggctcgg tgattccgtt cgtgggcctc ggcttcggcg cgctcttcta
     5101 cgacggcttc acgatggaca acacggcggt gctcgcgccg atgctctcgg gcggcgtccg
     5161 cgtcccggtg ggcgggatgg cggccgtcaa catgagcgtg ggttatcagc acgagtcgaa
     5221 tgcggacggc gagttcaacg cgagtgccaa ccggatcgtc gggtccgtcg gcgtgtccct
     5281 cttcccctgg cggacccact gacgaaattc gagcgcaagg gccggatagc ggcggcgcca
     5341 cgttcgcggt catgatcgag cctcacccgg tcgaagggag gcaccgacca tgaactatcg
     5401 cgtccgcggg ctctcgcccg atccgttcga acccctgttc caaatgagcg acgacgagct
     5461 ggccgagcgc ggcgcggtcc ggcgcgtggc ggacgagccg cacgcctatc cgtgccgcgt
     5521 gagcctggcg gaggccgagc cgggcgagga gctgctcctc cttccctacc gccatcaaac
     5581 gagcgcgtct ccgtaccagg cgagcggacc gatctacgtg cgccggaacg cgcgcgcctc
     5641 gttcgatgcg ctgaatcgga tccccgcgat gcaggagcgg cgcctctcgt cggtgcgggg
     5701 atacgacgcc gcgggattca tgcaggagtg cgacgtcgtc gcgggcacgg atctcgacgc
     5761 gacgatccgg cggttcttcg cgaacccgga ggtctatacg atccacgtcc acaacgcgcg
     5821 tcctggctgc ttcgcgtttc gggtggagcg ggcgtgaggc gagcgttcct cgtcgtcgcg
     5881 gttctggcgc tgtccgcggc gagctcccat gccgcgacgt cagagcgcag ctcggcgccg
     5941 gatcccgggc tcacgtcgtt ccgccaatgg ctcgacgcca accgcccggg ttacggatgc
     6001 gacgagggac ccgcgccgtt ccgcaacaag acggtcgaag cggcctaccc ggggcagcgc
     6061 ttctactacg tgctgaccta cacgcgcggc atccagcctc cgttcgagaa ctcggtctcc
     6121 ctggtggccg ccgtcgacga cagcggtcgc gtggctccgt tcctccccgg ccggccggcc
     6181 tcgtacagcc gcggcctcaa gcgcatccag tcctcgaagg acgccaggct cgccggcgcc
     6241 gcggtgttga tcctgggctc gtgcggcgag cgccgctggc ccatcgctcc ggagcgggtc
     6301 aaggcgaaga agaactcgaa ggggtggacg gcgacctact tctacgactc ctacaattcc
     6361 tcgtgggtct ccttcgacaa gaagggagcg gtgctcgcct tcgggggcag cgctccgccc
     6421 gtcccctaga ccgaagggtc tagccgctct tctgccgccg cgggcgtata ctcccccgca
     6481 tgtcgatctc cgccggggcc acgctcggcc cgtacgagat cctcgccccg ctcggctccg
     6541 ggggcatggg ggatgtgtac cgcgcgcgcg acccgcgctt ggggcgcgac gtcgccatca
     6601 aggtgctccc ccccgcgttc gccgccgatc ccaaccgcct ggcgcgcttc gagcaggaag
     6661 cccgcgccgt cgccgcgctc aatcatccga acatcgtcgc gatctacgac gtcggcaagg
     6721 acgccgaggg gcgcccctat ctcgtcaccg agctgatcga ggggacgacg ctccgcacgc
     6781 gtctcctgga cgagccgctg ccgctccggc aagcggtcga cttcgggctc cagatcgcgc
     6841 acggcctcgc ggccgcacac gacagaggaa tcgtgcatcg cgacctgaag cccgagaacc
     6901 tgttcgtcac gaaggatcgc cgcgtgaaga tcctcgattt cgggctcgcg aaggtggtcg
     6961 gaccccgcga gagtcgctcg cccgagtcga cgatcgcggt gggcgcgacc gaatcgggca
     7021 ccgtgctcgg caccgtcggc tacatggcgc ccgagcaggt gcgcggcgat gcggcggacc
     7081 atcggtccga cctttttgcg ttcggctgca tcctctacga gatgatctcg gggcggagag
     7141 cgttccacgc tctcacggcg gccgagacca tgaccgcgat cctccgatcc gatcctcagc
     7201 ccatgggggc gccggagcag gttccctcct cgatcgagcg cgtcgtgcgc cactgcctcg
     7261 agaaggctcc cagcgagcgc taccaatcgg cgcacgatct cgcattccag ctccagggcg
     7321 cgctcgaccc aagcgacggc gtggctcctg cgaggccaag ggggcgatgg ctcgcggtca
     7381 cggcgctcct cgcgagcatc gtcggcggcc ttctcgctgt cgcgcctcgg tggctcagcc
     7441 ctcccgccaa gccgcccgag ttccatcgca tcacgttccg cccgggcttc gcgtggtcgg
     7501 gacgcttcgc gcccgacgga cgcacgatcg tctacagcgc ggcgtggggc gaggcgccgc
     7561 tcgagacgta ctcgacgagg ccggagagcc ctgagtcgcg ccagctcgga ttgaagaacg
     7621 cgagcatcct ctcgatctcg aggtcgggcg agatggccgt gctcttgaac gcaagctacg
     7681 ccggaggctg ggcctatcgc ggaaccctcg cgcgggtccc cctcgaagga ggcgctccgc
     7741 gggaggtcct cgacagcgtt ctctgggccg actgggagcc gggcggccgc gatctcgccg
     7801 tcgtgcgcga gtccgggggc gagcggcgtc tcgagtatcc gatcggcaag gtcctctaca
     7861 aggcggcggg cacgatcggc gagccgcggt tctcgcccag gggcgatctc atcgcgttcc
     7921 tcgatcatcc gatcttcggc gacgatcgcg gctcggtctc ggtggtggat cgcgcgggca
     7981 aggtgcggcg actcaccagc gtgtggaaga gcatccaggg aatcgcgtgg gctcccggag
     8041 gcaaggagat ctggttcacg gcagccgacg agggcccgat tcgcgcgctc tatgccgtga
     8101 ccccgagcgg gcgcctgcgc accgtcatga atccgccggg atccctctcg ctccaggaca
     8161 tctcgccgtc cggcggcgtg ctcctcgccg aatggcggat ccgctacggc atcaccggac
     8221 gctccgggtc cgacgccggc gagcgcgatc tgtcgtggct cgactggtcc gccgtgcgcg
     8281 acttctccca cgacggaacg cagatcctgt tcagcgaagg aggcgaggga ggtgggccga
     8341 gctacggcgt ttacttgcgc ccgacggacg gctcgcccgc ggtgcggctc ggcgagggtg
     8401 agacgctcgc cctctcaccc gatgggcagt gggttctctc ggtgccacga ggcgacaagc
     8461 cgcacccggt gctccttccg acgggcgccg gccaggcgcg atccctccca gcgggaccgc
     8521 tccgcgaata tcagaacggc acgtggctca aggacggccg cgtgctcctg gaagcaagcg
     8581 agccggggca cgggaatcga tactacgtgc aggacgtgaa cggaggaatg ccgcaggcgg
     8641 tgacgccgga gaacgtgacc gggttcggat tcggggcgca cacggcggcg cccgacggcc
     8701 ggaacgtgat cgcgcgagga cccggcggat cgatcgagat gtggccgctc ggtccgggtg
     8761 agcccgtgcc ggtgagcggt ctcgcggccg gcgacgtgcc gattcgctgg gcggatgacg
     8821 gtcgcactct gtacgtggcg catgccgcgg cggggcggcg ggagctcgag ctcgcgcgcg
     8881 tggacctgcc tacggggcgg cgaacgtcgt ggaaggacct ggttcctcgg gacgcgatcg
     8941 gcgcgacccg gatcggatcc ccgatggtct cgccggatgg aagcgcgtac gcctacacgt
     9001 acggctcgca caccgccgac ctctatctcg tagaagggct ccgctaaggg ttagggggtg
     9061 ttctgggcca gccaggccac gcgtcccgtt tcgagcgagt acacacctcc gacgatcttc
     9121 agttttccct cggcgatgtg ctccgccaag atcggatgcg atgcggcgag ctgcgcgacg
     9181 accgcctcga cgttcaagcg gatcgcattg tccgagagat cgccctgcat cgagcgcgcc
     9241 ttctccaccg agggggtgat ggcctggatg agcgccggaa ggtgcccagg agcctcccct
     9301 ccttcgatcg ccgcgcccac cgcgccgcac gattgatgtc ccatgaccac gatgagcggc
     9361 acgtggagat gctccacggc gtactcgatg ctcgcgatct cgggatctcc tgcgatgttt
     9421 ccggcgacgc gcacggtgaa gagatcgccg aggcccttgt cgaacacgat ctccggcgcc
     9481 acacgcgagt cggcgcaggc caggatgacc gcgtgcggat gctggctggc cgcgagctcg
     9541 gtccgtcgcg ccgccgtctg gtgcggatgc gtggcgtggt ggctcgcgaa ccgctcgttg
     9601 cccgccttga gctcggtgag agccgaggtg ggcgtggggg ccgtcttggt tgccggtgcg
     9661 gcgcccagga cgaggattcc cgccaggatc gcggcgagac gggtggcttt catgactttg
     9721 aagctccttt cctggctgcg gccaatctcg atgcgggacc gcggaggatc atgtagggaa
     9781 tgagggcgag gaacacgggg accagcacga tctggagcgg ccggatcttg tggatcgcga
     9841 tgatctgata gatcgcgtcg agcaccatcg cgatcacgaa gatcttgccc acgtccttcc
     9901 agccctctcg aagcagagcg cctcggtgct ccgggtctcg cacgagggtc atcagatacg
     9961 gcgggcgccc ctcacgcccg tccttgatcc cggagcggat ggcgaagatc gccgccatcg
    10021 tcggctggag aaagagccgg agcgtgagcg gcccgtcggc ccggccgatc agctcgtccc
    10081 agaattgacg caggaattcg cccatggctc ctccgcgggt caggttcccg tcttcttgta
    10141 cccggagttc tcgatgacgg tcttgatccc cgtcgacttc acgtcgaaga cgatctgggc
    10201 cggataggtg atcccgccct cgagcgtccc gagcgtggca tccagagtta ccgcgtcatc
    10261 ggtcttcttg agataggagg cgacgctgag cttggtgagt tggttcttct cgagatcgac
    10321 ctcgatcccg aggacgtcgc cgggct
//
LOCUS       DASTKC010000214        25914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_175873, whole genome shotgun sequence.
ACCESSION   DASTKC010000214 DASTKC010000000
VERSION     DASTKC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 25914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25914
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175873"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFR25_08890"
     CDS             complement(<1..506)
                     /locus_tag="VFR25_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9941209.1"
                     /translation="MSTLIGSITAEYRRYKSLAEAALEQVPEAALSQSGPSGGNSLAT
                     ICWHVSGNLESRFTEFLTSDGEKPWRQREEEFAARYVTRAELMEKWNRGWSALLGTLD
                     TLTDADLSKSITIRGVALSVHEALHRSLAHTCYHVGQIVYVAHAIRGREWRYLSIAPG
                     QSDAYNANP"
     gene            615..1370
                     /gene="lexA"
                     /locus_tag="VFR25_08895"
     CDS             615..1370
                     /gene="lexA"
                     /locus_tag="VFR25_08895"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9941210.1"
                     /translation="MRMKPDPPKKGAGRPRRPVPPAHLNPDAAPSREEEILAFIAGSL
                     RERGYPPTIREIGKAFGIASTNGVRYYLDRLEQSGKIRRDRWTSRGIELQAAEGDVRE
                     RPMREPTPARLAPVPRRSTNEPVEIPILGRVAAGAPILAEENIEDVLLVDGAFVRPGK
                     HFALKVRGDSMKNAGIFDGDIVIVRHEAPVKSGEIAVVLIGEEATVKRIFPRRDKVLL
                     LPENDDYEPIEIRPSDPETRVAGKVVGVFRRLG"
     gene            1370..1666
                     /locus_tag="VFR25_08900"
     CDS             1370..1666
                     /locus_tag="VFR25_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941211.1"
                     /translation="MVHAHTFPKQEDGYFMIESIHDPIEVITLFHAGRMKPIRFRWKN
                     KIVKVAKVTGDWVRHEGQNKIHFFAILADNADYYEIRYDSREMTWQLTRVWMEG"
     gene            1673..2884
                     /gene="dinB"
                     /locus_tag="VFR25_08905"
     CDS             1673..2884
                     /gene="dinB"
                     /locus_tag="VFR25_08905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET9941212.1"
                     /translation="MRVVAHVDMDAFFASVEERMNPHLAGRPIAVGGDPKQRRGVIAA
                     ANYPARKYGLHAGMPTGEALRLCPELTIVEGNPQKYVHTSLQILEILKDYTPIVEPFS
                     IDEAFLDLTASPRLAASRGEGFNPADPADVLDAAIPTARAIQRAILRKTGLGATVGIA
                     PNKYIAKMASGVQKPRGLTVLTQERYREHFWPLSVKELWGIGEKTRLALDKLGIATVG
                     QLARFPREFLTYHFGMNGDHMREAAWGRDDGPVVPYYEGIPLKSMGHEITLSENVTSR
                     EKALTHLLRLSDQVGRRMRQDGYVGRVVTLKIRDTRFKTITRQRAVPAAMNDEEQIYR
                     TAIALLDENWDGRPLRLLGVSVSGLASNGEAIQPSLFDGDEHRKKMTAAVDSLRDRFG
                     DSALMRAGALE"
     gene            2900..3823
                     /locus_tag="VFR25_08910"
     CDS             2900..3823
                     /locus_tag="VFR25_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9941213.1"
                     /translation="MIMIGTSGYSFPDWVGNFYPLHIERSRMLDFYAKQFPAVEVNAT
                     YYRIPPPSTMHAMERKTPPGFEFVVKTHHDMTHKQSLDPELHDSFRRAVAPLTDAGKL
                     SGFLAQFPQGFHRTVEHERFILDLKRTLPEEVPLFVEFRHASWIADDLFPWLREHGLR
                     FVSVDEPDLPGLLGVYARATGDLAYVRFHGRNKRTWHTQTPAGSGIARDTRPKKPPTG
                     APGVDPSLGPLFAGTPDTPPARKVAERDRPLLRYDYLYSESELQEWVGKIRELAATTK
                     KTFVFFNNCHAGQAATSAKLMRRLLEGEGLL"
     gene            3820..6189
                     /locus_tag="VFR25_08915"
     CDS             3820..6189
                     /locus_tag="VFR25_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9941214.1"
                     /translation="MNRPSLFEAPPRARVETLAEFEALLTSLRSQRGRDSELVHLERL
                     PERPAVYGDLATPLPAPLHAVLTAQGIERLYSHQARAVDLARAGSNVVIATGTASGKS
                     LAYHLPILETLLREPGSVALYLFPTKALAQDQLRGLTRLAEAAPQLRRAVIAGTYDGD
                     TPGHTRRRFREQGNAILTNPDMLHQGILPYHSRWSRVFSNLKYVVVDEVHTYRGIFGS
                     HVANVLRRLRRVARHYGSEPRFLLSSATIRNPGDLAERLVGAPVQVVNEDGAPRGPKL
                     FAFWNPRIESGDAASRGSTSVEAERILVSLMRRDVQSIVFTRSRVAAELIYRYARERL
                     DRDHKGLAEKLSPYRGGYLPEERRAIERRLFDGDLKGVISTNALELGIDVGSLDAAIL
                     VGFPNTIASTWQQAGRAGRSREPALAVVVAYDDPIDQYLMRHPEYFFSQSPESAVLDP
                     ENPYVLASQLACAAYELPLTAEDEAQFGARAPEIASLLEEDGALKSLDGSRYWSSTEY
                     PAGRVNLRTISDDTFTIVDAGRNNAVLATVDSISAPELVYPEAIYLHEGDTHFVRELD
                     QAQKVAYVERREVDYYTQPVLDTRLRLLDQRETKRWNESEVGLGTADVSWATVAFKKI
                     RFRSLDAIGYHPLDLPRQNLETVALWFRPEDGVRAQVRFAGLNPHEAMSGLRNLLITL
                     LPLHVMCDRPDLGGILDSSNLGDPAIFLYDRYPGGLGYAERGYALVEDLMRAALRMVE
                     ECPCEMGCPSCVGLPVLRPAQQQDYDLHGGWPIPNKAATELLLNRLLGR"
     gene            6201..7208
                     /locus_tag="VFR25_08920"
     CDS             6201..7208
                     /locus_tag="VFR25_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET9941215.1"
                     /translation="MLSRSLRDRISGLIRDGFGRGGAPSGNGAAGHSGNGHNGHHPAD
                     DRSIIPSGIEDLLPGGIIHGPHGAAFVHERLYTDLGERPELVLARYEALCALEPDREP
                     EPAVVAADTARPYDPALEPRVVYDPEWQPETAPLDLPTIVARMEWPERGLFRRFGFRK
                     MLFLDLETCGLTACPVFLAGVGLIGENDLVLRQIFARDYAEERALIHEMARLIAEADF
                     LVSFNGKAFDVPFLRDRAAHHRMPFPSSAPHLDLLWLARRRWKHTLPDCKLKTLEWRI
                     LRRRRSGDVGGSDIPGLYHHFVQGGEAHRLVPIFHHNMLDVVAMVELLPALFDPDAVV
                     Y"
     gene            complement(7205..7753)
                     /locus_tag="VFR25_08925"
     CDS             complement(7205..7753)
                     /locus_tag="VFR25_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9941216.1"
                     /translation="MIVPAIRTERLTLYPATIPLLEAERDPQGEFAALLQAEVARPWP
                     PPLNDEASRAWMIDLLRRVPEPGPWGMYYFLLRREGELPIAIGNGGFKGPPRDGSVEI
                     GYSIVESYQRRGYGAEAAGGLVRRAFEDPSVQRVLAETFPHLAGSIGVLRKLGFTRRG
                     VGTEEGAILFELPHQDWERRKA"
     gene            complement(8245..10308)
                     /locus_tag="VFR25_08930"
     CDS             complement(8245..10308)
                     /locus_tag="VFR25_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9941217.1"
                     /translation="MASPTQEEPAQPELPELPPDAPPPDNLEPRWLRAIRRAFEVVIG
                     PARRLTDPHLFHKISLAAFLAWVGLGADGLSSSAYGPEASFLALHGNDFLAIPLAILT
                     AITVVVIAASYMRIIERFPAGGGGYVVATKLLGPTAGLVSGSALLVDYVLTVAISLAA
                     CSESLFSLLPPGLHFASLPFTFGLLGLLTLLNLRGVRESVKIISPIFLVFVLAHAVAI
                     ISGIVLNIHQIPGEVRHLHEQSSAAIATTGWIPLLLTLFRAFTLGGGTYTGIEAVSNG
                     VPMLREPKVETAKTTMRYMAFSLAVIAAGILVNYLLYRVQPVPGKTLNAVLWSALAGH
                     VFAPGSMVGAVLVAITLISAGALLFVAAQTGFLGGPRVLVYMAFDRWVPSRFGNLSER
                     LVTSNGVLFMALSAAFVLMLTQGEIHVLVVLYSINVFLTFTLAQLGMVRDSFIARREG
                     HTWKRALAINGIGFFMTAAILVGTVSFKFMEGGWSTLVATGAICAVCVSVRKHYVNTA
                     ATLQRLDESLLTVPLPTGEPTESPLKKNAQTAVLTVVGFGGLGIHSLLNIFRIFPNQF
                     KQVVFISVGAIDSGRFKGLDEIEALKASTEAELAKYVAFARRLGLPAEGRCAIGTDIV
                     DELEQLCLQVSEDYPRSVTFGGQLVFQKERVVLRWLHNQTCPALQRRLAFHGKPMVIL
                     PVRVY"
     gene            complement(10404..10607)
                     /locus_tag="VFR25_08935"
     CDS             complement(10404..10607)
                     /locus_tag="VFR25_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET9941218.1"
                     /translation="MRKRGTVKWFNEAKGFGFIQQAGGEDVFVHYSAIQGDGFKTLAE
                     GQAVEFEIVEGPKGKQAANVTAV"
     gene            11025..12386
                     /locus_tag="VFR25_08940"
     CDS             11025..12386
                     /locus_tag="VFR25_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941219.1"
                     /translation="MTASIREVLLGMACGALLAAPAKAADSAGARVGTAVSRGAPEVV
                     IYNDDRALVRQPVDLPAGASRVEMGGLPARIDSTSIRLDGVRVLRQAYQYDVWDADLV
                     FRRCLGDSIFYRYGGRRYRGVLAGIDGNELFILRADSTDVLTIMNRQQLSEIELPRAR
                     RLRTTPTLEWELESGKARSANLSYVTAGLGWTAEYAAVLDKDERGLELSGWAIIANRS
                     GTAFRDAKVMLVAGEVNPGGMLPDRGLAAEGAPAATPSTAPELFAYRSYPLDRALSLD
                     DLETAQVPIVGPARVAARRTYRFDGARDGSKVRVELEFTNDKTSGLGAPLPEGRVRIY
                     AAGAGGGQALAGEDRIQHTAAGEKVRLLSGVAFDLVGERERVRHTRVSRNVTEDAYRI
                     VLRNHGAKAAKVTIEETLYGNWEITQKSSDFRRPDADTAEFDLDVPSGGQAEVTYTVR
                     YTF"
     gene            12397..14094
                     /locus_tag="VFR25_08945"
     CDS             12397..14094
                     /locus_tag="VFR25_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9941220.1"
                     /translation="MKLLGAALLLAVALPSAGRANEASPSPYWVFFRDRPAASGAAAI
                     LTPRSLARRAKAARARGEAPPAAVDDRDRDPDVAYVRAVGDAGAILRTKSRWLNAVSV
                     DADSAVLDRIRALPFVTGVDRVAVLTLPEASADVATSPEVATSPDKPTDADAVEYGDS
                     YHQLEMLGVPEAHALGDHGQGVLICVLDSGFERSHEVFDRLVVRDERDFVFDDRDTGY
                     DPGQDLLTQADHGTSVLSVLAGYAPGRLIGPAYESEFLLGKTERIGSELRSEEDHWCE
                     AVEWAEAEGADIVSSSLSYPLWYRLQERDGRTAENSRVANIALERGVLVVNSTGNYGP
                     GPMTLSPPSDAPGVLAVGSVDANGRLSPFSSIGPTWDQRIKPDLVAMGSAARMARART
                     RHDYMTANGTSFSTPLIAGCAALVLSAHPDWSPEAVWDALAMSADRTSRPDPRYGWGV
                     PNVLEAILYPQIEGRITDVSTPGVPTVGKPATVSWEPAPGAPAPSAADTLAAPSDSPR
                     RGSVRADSTGAYVIPNLPSGTYRLTIEAPGYFSVQSEPLQVPPALGDVDFALRYRGE"
     gene            14096..14464
                     /locus_tag="VFR25_08950"
     CDS             14096..14464
                     /locus_tag="VFR25_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9941221.1"
                     /translation="MPEYPYSTHLNILKGPLETIDVGQLADECEERWWNQTLCQVNAS
                     VVRLGVIQGEYHWHKHDHDDEFFYVVEGTLLLDLEGRTITLEPRQGFVVPKGVVHRTR
                     APERTVILMVENAGIVPTGD"
     gene            complement(14474..16120)
                     /locus_tag="VFR25_08955"
     CDS             complement(14474..16120)
                     /locus_tag="VFR25_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HET9941222.1"
                     /translation="MWRLTVLALALTLGPPCSYAKEATPPPRAESSVLDSLVVFHTND
                     THAQLMPYTAKGGPPAGGAAARAALLGRVRGGDRVRTLLLDAGDVFQGTPYFTFYRGR
                     PDYTLMTRMGYDAGILGNHDLDDGPAAWRRAASEARFPLLSANLFGAAESTWALGDSV
                     SAEDRKGARWVGRVKVPDGARLRYLTQRFTIATLPSGLRVALFGLVTADVTTIVAVRP
                     NAGVAVGDPIAVARRLVPELRRQADVVIAVTHLGVEVDRKLAERVPGIDLIVGGHSHT
                     ALRKPILVHNATPNGYDGTVIVQAGSRGVYLGRIALYLKGRTPVRYAGSLLPVNPANG
                     EDASVLAYLKPLADSMSVLVDRAVFETVDAVPSSGLRDGETPLGNFVADVTRDVGNAD
                     VGIVNSGGIRAPLPRGRVTAGDIYSVVPFDNEVAVVMMPGWQLRQLLDFGARRLGKGG
                     FPQVSGVSFTIRGDHATDIKVGGRVLDSERVYRVATIDFLIGGGDGYTIFAKAGEPER
                     TGVMLRDAAVDFLQRNPRYEFRKDGRIHWEGSMRGLGGMR"
     gene            16354..16689
                     /locus_tag="VFR25_08960"
     CDS             16354..16689
                     /locus_tag="VFR25_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HET9941223.1"
                     /translation="MPEVDKVLKCRDCGEEFTFTAGEQAFYKERGFQHEPTRCRRCRD
                     EKKRVATGAPSGMMASAGSNASSREFHDAVCTSCGTPTQVPFRPTPGKPVYCRDCFQS
                     VRSPKGVSM"
     gene            16755..16964
                     /locus_tag="VFR25_08965"
     CDS             16755..16964
                     /locus_tag="VFR25_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9941224.1"
                     /translation="MRHRGSVKWFSEAKGYGFIREDSGEEFFVHHSAILADGFRTLNE
                     GQVVEFDPTEGQRGKQAANVVKIDA"
     gene            17099..19849
                     /locus_tag="VFR25_08970"
     CDS             17099..19849
                     /locus_tag="VFR25_08970"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9941225.1"
                     /translation="MRPPFRLIPFSLLALVALAVPALAAPTLVRTLPNKSTLVLRENR
                     TRPIVSIQAWIKSGSRDETRSERGAAVVLQQVMLRATKKRVADVMEKDIELYGGSFGN
                     EVGYDYSMFEITLPARYFKAGADILSEIVTQPRLDTRDIQESIGIARDQAKYVLRQAE
                     LATNNPVRDALWPDTPLGSPSTVPELELAAVTMPLAVRYYKTHYVSENLMLVVVGDVD
                     PAEVPSVMEQAFASMPQGKAPSHPKVKAKGAPSTKISFADPPPDAGAAFAVGFRAPAW
                     GTADAVALDVLLAALIDSPHSRILTRPVEGSDAFTNLTAQRSFDVDGGTISLSGRIDP
                     MRAQDAEGALIALLAQARSAPISQSEVDDAVDAVVSRDGFYASDMNGLARATALAYLR
                     GKPGADEVYFDRLRTIRPEDLTGVAAQYLDLDHAAVVEMMPRSAADSLRAKSGFEGRI
                     KEKLGINQAAYGKGPKVTQSSPQDRQKRIDAPLAKIPSTAPDMGRARVEKSTLAGGLR
                     VLTSQDRSAPTVSVGVYLAGGTRYENETNNGVTSLLRESILTSADPKADHGAAYRHSL
                     PELGRFQPYQDKDMWGFSVTVPSDEWKEAVTRLGTMLSHPEIDSVTVDATRLYVLDAL
                     NKWSENDQAQRSWSIFPTKYQVSGYRLPALGTRYNLMTMPYGTIMDWYKTFVVRGNLV
                     VSVFGDVTPSEVGPAVDHAFADLSSKPFQPPPVAKEPPFEDFREKWDLGAGTASTVNL
                     AFNGPPARSRDIPTFYVINSLLSGPRGWFETYMNSNEALFKGSSSIVSQSIDESPLIA
                     SVVIAGPMVEEDGVKLLFRQFKKVAFYEMTAEEADTLRFAKIHAAGTYLSLLGTNTSR
                     AFQWGRAEVFGLGMEYPLTLPVKIDAVTAEDVQAVGLAYFEKDQFNRKPYAITETRPG
                     GW"
     gene            19851..20807
                     /locus_tag="VFR25_08975"
     CDS             19851..20807
                     /locus_tag="VFR25_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941226.1"
                     /translation="MASEAVAVIATVRNEAASIDRLLDSLLSGSRTPDEIVIADGGST
                     DGTLERLRARAGSEARIRVLVAPGNRSVGRNAAVRAARSEVIACTDAGVDVDREWLDR
                     ITRPFANPDVDVVAGFYQPAGETSFERAAGVVSAPRLEEIDLERFLPSTRSVAFRRAA
                     FERVGGFDEALWHNEDTPFALALKEAGCQFAFAPDAIVRWRPRGDLRAFMHQHRRFGT
                     GDGEARVQSWFYATIAVKYAMAVVLFVAGFWLRAAWWLLGLGVALFIATQARRGEGRL
                     GLWERLVGVPALKVAYDLAYLSGYVKGRLGPPASARPAPGRS"
     gene            20819..21022
                     /locus_tag="VFR25_08980"
     CDS             20819..21022
                     /locus_tag="VFR25_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET9941227.1"
                     /translation="MAEGTRVFRRSSNRMIAGVCGGIAEYFGWPPDRVRIAYVLLSIL
                     SIAFPGILVYLLLWLVMPGPAGT"
     gene            21022..21420
                     /locus_tag="VFR25_08985"
     CDS             21022..21420
                     /locus_tag="VFR25_08985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET9941228.1"
                     /translation="MGFCVFCEIVAGRIPSYKVWEDRDFIAFLDIKPIRPGHILLVPR
                     EHHEAVFDMPPDLYDRTFRAARRLAAPLGKHFNVPRVGIVVEGFGVAHAHVHLVPISE
                     AGQLNPALASEASGEDLKRTRDALAPALEH"
     gene            complement(21417..22217)
                     /gene="cdaA"
                     /locus_tag="VFR25_08990"
     CDS             complement(21417..22217)
                     /gene="cdaA"
                     /locus_tag="VFR25_08990"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET9941229.1"
                     /translation="MIEIRPQFLVDLIDVLAVAFLFYRLFAMIKGTRAAQMFVGLVFI
                     VIASIVAQWFRLSALNWIIGSLKTVWVILFVILFQPELRAVLTHIGQNRLLRALIRVG
                     EGGVLKEILSAVEEMSRERRGALIVVERDMGLRDYIETGTKLDASVTKELLETIFTPH
                     SPLHDGAVVIRGDQISAAGCILPLSATAGLSPLLGTRHRAALGLAEETDACVFVVSEE
                     TGAISIAHKGELKWNLDEGQLRSELAAIFNPRPEDTPDEPEEEIAAGA"
     gene            complement(22214..23152)
                     /gene="folP"
                     /locus_tag="VFR25_08995"
     CDS             complement(22214..23152)
                     /gene="folP"
                     /locus_tag="VFR25_08995"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET9941230.1"
                     /translation="MRQNDVVERAEVARAPLIFAHRFGRWDLTRTPRVVGILNVTPDS
                     FSDGGRFVEPREAARHAERMIEEGADAIDVGGQSTRPGAGEPVGAEEEWTRIGAVVKA
                     LRARAGSVPLSVDTYRAEVARRALAEGAAMINDVTALAYEPGLADVVAEAGAGLVLMH
                     SRGGPSGFHDAEMKDYDDVVDEVRWELYMAMRRALVRSVPEETIALDPGVGFSKRPVH
                     SVGVLRGLPRLTDLGRPLYIGVSRKSFLGALTDEPVDDRLAAGLGATVAAYALGARIF
                     RTHDVRATRDALTLAEALLSEGAVVETARALTEKRA"
     gene            complement(23157..25226)
                     /gene="ftsH"
                     /locus_tag="VFR25_09000"
     CDS             complement(23157..25226)
                     /gene="ftsH"
                     /locus_tag="VFR25_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9941231.1"
                     /translation="MNWDPRPYARSNKPSNGRPSRRPAPPPRPPQRKPTPFGPARPLR
                     TALLWVILVLAVLVFVQIYTEMRAKTHEIPYSEFLQQIDLANIKSSTIIEREVVGELK
                     QSQLTHVEGRPVSYVFFKVYLPAEDKDIGRAILAKDPNAMVNSKPQGMNWWSIALGYI
                     PFVALLVLWMVMIRQIQSGGASAMKFGKSRPKVALESSPKVTFKDVAGADEAKQELEE
                     IIEFLRDPKKFQRLGGRIPKGALLLGPPGTGKTLLAKAVAGEAGVPFFSLSGSDFVEM
                     FVGVGASRVRDLFDQGKRNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEM
                     DGFESNDGVILIAATNRPDVLDPALLRPGRFDRQIVVDVPDVRGREGILRVHTRNIPV
                     GDDVNLNTIARGTPGMVGADLANLVNEAALLAARRNHKRVNTLDFEDAKDKVMMGTER
                     RSLVISDAEKRSTSYHEAGHALVAWLQPGSDKVHKVTIIPRGRALGLTAYLPQDERHS
                     FSRDYWLQILTHMMGGRAAEQIVLNQLTTGASDDIRRATNIARRMVCEWGMSERLGPI
                     TLGSKEEFVFLGREISQHKDFSEKTAIAIDEEVRGLVEGASNRARQLLTDNLDKLHLL
                     ALALLEREVLDGEEVEKVLRGEKLEPIRRDGGGDNRPTPAAAPVEEPKREKAPPRVHP
                     TESPGLA"
     gene            complement(25310..>25914)
                     /locus_tag="VFR25_09005"
     CDS             complement(25310..>25914)
                     /locus_tag="VFR25_09005"
                     /inference="COORDINATES: protein motif:HMM:NF020741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941232.1"
                     /translation="GAGLRGLSGMPVRGKVRGTRIARPLLDVTREQIQEYLRRRGVNY
                     RVDSTNETSLPARNFIRRDVLPLVRSRLNPSAREALLRTASAARDADEILVAIARERL
                     AEAIVLSEPGQIALDATRLVQYPKPLRAYVFRLALQELTGSLREIAGAHIESLLTLAT
                     TTRPRSLDLPGQVRAYRDGGRIVLDRRMREAEAIREHPRA"
BASE COUNT         4195 a         8854 c         8745 g         4120 t
ORIGIN      
        1 gggttcgcgt tgtatgcgtc cgattgcccc ggagcgatgc tgagataccg ccactcgcgt
       61 ccacgaatcg cgtgcgccac gtagacgatc tgccccacgt gatagcacgt gtgcgcgagc
      121 gagcgatgga gcgcctcgtg cacggagagc gccacgccac ggatcgtgat cgacttcgag
      181 agatcggcgt cggtgagggt atcgagggtg ccgagaagag cgctccaccc ccgattccac
      241 ttctccatca gctccgcgcg cgtgacgtag cgagccgcga actcctcctc gcgttgccgc
      301 cacggcttct cgccatcaga agtgaggaac tcggtgaagc gcgactcgag gttgccggac
      361 acgtgccagc agatcgtggc gagcgagttg cccccagacg gcccggactg ggagagcgcg
      421 gcttccggca cctgttcgag cgcggcttcg gccagggact tgtagcggcg gtattcggcg
      481 gtgatggagc cgatgagagt gctcatgggg atcactcctt ggaccacaga cgcagaatcg
      541 tatgcgccag cagattacga caaaatcgcc cccacccctt gacacctgca caaatgtgca
      601 gtatcttctc cggcatgagg atgaagcccg atccgcccaa gaagggggcg gggcggccac
      661 gccgccctgt tccccccgcc catctcaatc cggacgccgc cccgagccgc gaggaggaga
      721 tcctcgcctt catcgcgggc tccctccgcg agcgcggcta tcccccgacc atccgcgaga
      781 tcggcaaggc gttcggcatc gcctccacga acggcgtccg ctactacctc gaccgcctgg
      841 agcaatccgg aaaaatccgc cgcgaccgat ggacgtcgcg cgggatcgag ctccaggcgg
      901 ccgaggggga cgtgcgggag cgaccgatgc gggagccaac ccctgcccgg ctcgctccgg
      961 tcccccgccg ttcgaccaac gaacccgtcg agatccccat tctcggacgt gtggccgccg
     1021 gcgcgccgat ccttgccgag gaaaacatcg aggacgtgct ccttgtggac ggcgcgttcg
     1081 tgcggcctgg caagcacttc gccctgaagg tccgggggga ttccatgaag aacgcgggga
     1141 tcttcgacgg cgacatcgtc atcgtgcgcc acgaagcccc cgtcaagagc ggcgagatcg
     1201 ccgtggtcct gatcggcgag gaagccaccg tgaagcggat ctttccgcgg cgcgacaagg
     1261 tgctcctcct ccccgagaac gacgattacg agccgatcga gatccgcccg tccgatcccg
     1321 agacgcgggt ggcgggcaaa gtggtcggcg tcttccggag gctcggctga tggtccacgc
     1381 gcacacgttc ccgaagcagg aagatgggta cttcatgatc gaatccatcc acgatcccat
     1441 cgaggtgatc acgctgttcc acgcggggcg catgaagccg atccgcttcc gctggaagaa
     1501 caagatcgtc aaggtcgcga aggtgacggg ggactgggtc cgccacgaag ggcagaacaa
     1561 gatccatttc ttcgccatcc tcgccgacaa cgccgactac tacgagatcc gctacgactc
     1621 gcgcgagatg acatggcagc tcacccgggt gtggatggag ggatgatcgt cggtgcgcgt
     1681 cgtcgcccac gtggatatgg acgcattctt tgcgtccgtg gaggagcgga tgaacccgca
     1741 tctcgcgggg cgcccgatcg ccgtgggtgg agacccgaag cagcgccgcg gcgtgatcgc
     1801 ggcggcgaac tacccggctc ggaagtacgg ccttcacgcg ggcatgccga cgggggaagc
     1861 gctccggctc tgccctgagc tcacgatcgt cgaagggaat ccgcagaagt acgtccacac
     1921 gtcgctccag atcctcgaga tcctcaagga ctacacgccc atcgtggagc cgttcagcat
     1981 cgacgaggcg tttctcgacc tcaccgcttc gccccggctc gcggcgtcgc ggggggaggg
     2041 cttcaatccg gcggatcccg ccgacgtgct cgacgccgcg atcccgaccg cgcgcgcgat
     2101 ccagcgggcc atcctccgca agaccgggct cggcgcgacc gtggggatcg cgccgaacaa
     2161 gtacatcgcg aagatggcga gcggcgtgca gaagccgcgc gggctgaccg tgctcacgca
     2221 ggagcgctac cgggagcact tctggccctt gagcgtgaag gagctgtggg gaatcgggga
     2281 gaagacgcgc ctcgcgctcg acaagctggg gatcgctacg gtcgggcagc tcgcccgttt
     2341 cccgagggaa tttctgacgt accatttcgg gatgaacggg gatcatatgc gggaggcggc
     2401 gtggggccgc gacgacggcc cggtcgtgcc ttactacgag ggcatccccc tcaagtccat
     2461 ggggcacgag atcacgctgt cggagaacgt gacctcgcga gagaaggctt tgacccatct
     2521 cctcaggctg agcgatcagg tgggacggag gatgcggcag gacggctacg tggggagggt
     2581 cgtcacgctc aagatccgcg atacccggtt caagaccatc acccggcagc gcgcggtgcc
     2641 ggctgcgatg aacgacgagg agcagatcta ccggaccgcg atcgcgctcc tggacgagaa
     2701 ctgggacggg agaccgctcc gcctcctcgg ggtttcggta tcgggtctcg cgtcgaacgg
     2761 tgaggcgatc cagccgagcc tcttcgatgg cgacgagcac cggaagaaga tgaccgccgc
     2821 ggtcgactcg cttcgcgacc gattcggcga ttcggctctc atgagggcgg gcgcgctcga
     2881 gtaggcgctc ggttcgccca tgatcatgat cggcacctcg ggctattcct tccccgattg
     2941 ggtggggaac ttctatcccc tccacatcga gcgcagccgc atgctcgatt tctacgccaa
     3001 gcagttcccg gcggtcgagg tgaacgcgac ctactaccgc attcccccgc cctccacgat
     3061 gcacgccatg gagcgaaaga cgccgccggg gttcgagttc gtggtgaaga cccaccacga
     3121 catgacccac aagcagagcc tggatccgga gctccacgac tcattccggc gcgcggtagc
     3181 gccgctcacg gacgcgggca agctgagcgg atttctcgcg cagttcccgc agggattcca
     3241 ccgcacggtg gagcacgagc gcttcatcct cgatctaaag cgcacgctcc ccgaggaagt
     3301 gcccctattc gtcgagttcc gccacgcgtc gtggatcgca gacgacctct tcccgtggct
     3361 tcgcgaacac gggcttcgct tcgtgagcgt ggacgaaccg gatctaccag ggctgctcgg
     3421 cgtctacgcg cgcgcgacgg gggaccttgc ctacgtgcgg ttccacgggc ggaacaagcg
     3481 gacgtggcac acgcagacgc cggctgggtc cggtatcgcc cgcgacaccc gcccgaagaa
     3541 gccaccgact ggcgctcctg gtgtcgatcc ctcgctgggc cctctcttcg cgggcactcc
     3601 cgacacgccg ccggcgcgca aggtcgccga gcgcgatcgc cccctcctcc gctacgacta
     3661 tctctacagc gagagcgagc tccaggaatg ggtcgggaag attcgcgagc tggcggccac
     3721 gaccaagaag acgttcgtct tcttcaataa ctgccatgcg gggcaggccg cgacgagcgc
     3781 caagctcatg cgccggctcc tggaagggga gggactcttg tgaaccggcc gagcctcttc
     3841 gaagcgcctc cgcgagcccg ggtcgagacg ctggccgaat tcgaggcgct cctcacgtcg
     3901 ctccgctcgc aacgtgggcg cgactccgag ctggtccacc tcgagcgcct tcccgagcgt
     3961 cccgccgtat acggcgacct cgcgacgccg ctccccgcgc cgctccatgc ggttctcacg
     4021 gcgcagggca tcgagcgcct ctacagccac caggctcgcg cggtcgacct cgcgcgggcg
     4081 ggctccaacg tcgtgatcgc aaccggcacc gcgtcgggga agtccctcgc gtaccacctc
     4141 ccgatcctcg agacgctgct tcgagagccc ggctcggtcg cgctctatct cttcccgacc
     4201 aaagcgctgg cccaggacca gctccgcggc ctcacgcgcc tggcggaagc ggcgccccag
     4261 ctccgccgcg cggtcatagc cggcacgtac gacggcgaca cgcccggcca cacgcggcgg
     4321 cgctttcgcg agcaggggaa cgccatcctc acgaacccgg acatgctcca ccagggcatc
     4381 ctgccgtatc actcgcgctg gagccgcgtc ttctcgaatc tcaagtacgt cgtcgtcgac
     4441 gaggtccaca cctaccgcgg catcttcggc tcgcacgtgg cgaacgtgct ccgccggctc
     4501 cggcgtgtcg cgcgccacta cggttccgaa cctcgcttcc tcctctcctc ggccaccatc
     4561 cggaacccgg gcgatctcgc ggagcgcctc gtgggagcgc cggtgcaggt ggtcaacgag
     4621 gacggcgcgc cgcgggggcc caagctcttc gcgttctgga acccacgcat cgagagcggc
     4681 gacgccgcgt cacgcgggag cacctcggtc gaagcggagc gcattctcgt ctcgctcatg
     4741 cggcgcgacg tgcagtcgat cgtctttacc cggtcgcgcg tcgccgccga gctcatctat
     4801 cgctacgcgc gcgagcggct cgatcgggac cacaagggcc tcgcggagaa gctgagcccc
     4861 tatcgcggcg gctatctccc cgaggagcgc cgcgccatcg agcgccggct cttcgacggg
     4921 gatctcaagg gcgtgatctc gaccaacgca ctcgagctgg ggatcgacgt cggctccctc
     4981 gacgcggcga tcctcgtcgg gtttccgaac acgatcgcga gcacctggca gcaggcggga
     5041 cgggcggggc gctcgcgcga gcccgcgctt gcggtcgtgg tcgcgtacga cgatccgatc
     5101 gaccagtacc tcatgcgcca cccggagtac ttcttcagcc agtcgccgga gagcgcggtg
     5161 ctcgatccgg agaatcccta cgtgctcgcg tcgcagctcg cctgcgccgc gtacgagctt
     5221 ccgctcacgg cggaggacga agcgcagttc ggcgcgcgcg ctcccgagat cgcctcgctc
     5281 ctcgaggagg atggggccct caagtcgctc gacggatcgc gctactggtc ctcgaccgag
     5341 tacccggcgg ggcgggtcaa tctccgtacg atctcggacg acacgttcac catcgtggac
     5401 gccgggcgca acaacgcggt gctcgcgacg gtggattcga tcagcgcgcc ggagctggtc
     5461 tacccggaag cgatctatct gcacgaaggc gacacgcact tcgtccgcga gctggatcag
     5521 gcgcagaagg tcgcgtacgt cgagcgtcgc gaggtggact actacacgca gccggttctc
     5581 gacacgaggc tccggcttct ggaccagcgc gagaccaagc gctggaacga atccgaagtg
     5641 ggcctcggca ccgcggacgt ctcctgggcc accgtcgcgt tcaagaagat ccgcttccgc
     5701 agcctcgacg ccatcggcta tcacccgctc gacctgccgc ggcagaatct cgagacggtg
     5761 gcgctctggt tccgccccga ggacggcgtg cgcgcccagg tgcgattcgc cggcttgaat
     5821 ccgcacgaag ccatgtcggg ccttcgcaat ctcctcatca cgctcctacc gctccacgtg
     5881 atgtgcgatc gccccgatct gggaggcatc ctcgacagct ccaatctcgg cgatcccgcg
     5941 atcttcctct acgaccgcta tccgggcggg ctcggctacg cggagcgcgg ctacgcgctc
     6001 gtggaggacc tcatgcgcgc ggcgctccgg atggtggagg agtgtccctg cgagatgggc
     6061 tgcccgtcgt gcgtcgggct cccggtgctg cgtccggcgc agcaacagga ctacgacctg
     6121 cacggcggct ggccgattcc gaacaaggcg gcgacggagc ttctcctcaa ccggctgctc
     6181 gggcgctagc ggggcggcgc gtgctctccc gctcgctgcg cgatcgcatc tcggggctga
     6241 tccgggacgg tttcggccgc ggtggagcgc cgtcgggaaa cggcgcagcg ggacactcgg
     6301 ggaatggcca caacgggcat cacccggccg acgaccggtc gatcatcccg agcggcatcg
     6361 aggaccttct gcctggcggg atcatccacg gaccgcacgg cgccgcgttc gttcacgagc
     6421 gcctctacac cgatctcggc gagcggccgg agctggtgct cgcgcgttac gaggcgctct
     6481 gcgcgctcga gccggatcga gagcccgagc ccgcggtggt cgcggcagac acggcacgcc
     6541 cctacgatcc agccctcgaa ccgcgcgtcg tctacgaccc cgagtggcag cctgagaccg
     6601 ctccgctcga tctccccacg atcgtcgcgc gcatggaatg gcccgagcga gggctcttcc
     6661 gccgcttcgg atttcgcaag atgctcttcc tcgatctcga gacctgcggg ctcacggcgt
     6721 gccccgtctt cctcgcgggg gtgggcctga tcggcgagaa cgacctcgtg ctgcggcaga
     6781 tcttcgcgcg ggactacgcc gaggagcgtg cgctcatcca cgagatggcc cggctcatcg
     6841 ccgaggccga cttcctcgtc tccttcaacg gaaaggcgtt cgacgtgccg ttcctgcgcg
     6901 atcgcgccgc gcaccaccgg atgccgtttc cctcgagcgc gccgcacctc gatctcctct
     6961 ggctcgcgcg gcgtcgctgg aagcacacgc tccccgactg caagctgaag accctcgaat
     7021 ggcgcatcct tcggcgccgg cgttcgggag acgtgggagg gagcgacatc ccggggctct
     7081 atcaccattt cgtgcagggt ggggaagcgc accggctcgt gccgatcttc caccacaaca
     7141 tgctcgacgt ggtcgcgatg gtcgagctct taccggccct cttcgatccg gacgcggtgg
     7201 tgtactaggc ttttcggcgc tcccaatctt ggtgcgggag ctcgaacagg atcgcgccct
     7261 cctcggtccc gacgcctcgc ctcgtgaatc cgagcttccg gagcacgccg atcgagccgg
     7321 cgagatgcgg gaacgtctcg gcgaggacgc gctggacgga tggatcttcg aacgcgcgtc
     7381 gcacgagccc tcccgccgcc tccgcgccat atccccggcg ctggtacgac tccacgatcg
     7441 agtagccgat ctccacggag ccgtcccggg gaggcccctt gaaccccccg tttccgatcg
     7501 cgatcggtag ctcgccttcg cgccgaagga ggaagtagta catcccccac ggtccgggct
     7561 cggggaccct gcggaggagg tcgatcatcc acgcgcgcga cgcttcgtcg ttcaagggcg
     7621 gcggccaggg cctcgcgacc tcggcctgaa gcaaagcggc aaactctccc tgcgggtccc
     7681 gctcggcctc gagcagcggg atggtggcgg ggtagagagt cagccgctcg gttcgaatcg
     7741 cggggacgat cacggtgtca ccatcgcgtg aaaatcgacg gccgcgccgc ggccggccgc
     7801 cgcgcggccc accgtcgaac gcgcgttgaa cggcgtcaag gttccgacgc ttcggagtcc
     7861 gacaccgcga ctcgcggcca acctacccgt actgagcttc aacgagttac atccctctcg
     7921 cgtcgagaac gacaggcacg atccctgcct tgctccccag cgtgtcgccg cgctcgcgac
     7981 cctgcatgcg cgcgccggtg gcggcacaat ccgcgaaggc gtcgcgggcg cgggccgtgt
     8041 ggacccaagt gaagcgctgc ggtgcggaga gacgccgcga tcgcttccag ggcaccgccg
     8101 agggacggtg tccaccaggg gagggggccc gaaacaacga cgcgcgtccg cgcgggcgat
     8161 ggacgcggag cggggttcgg tccccacctc cagtcgattt ctgattggac cccgcccgcg
     8221 tctttttgtc ccaagcgacc gccgctagta cacccgcacc ggcaggatca ccatcggctt
     8281 tccgtggaac gcgagacggc gctggagcgc cgggcaggtc tggttgtgga gccaccggag
     8341 gaccacgcgc tccttctgga acacgagctg gcccccgaac gtgaccgagc gcggatagtc
     8401 ctcactcacc tgcagacaga gctgctccag ctcgtcgacg atgtcggtcc cgatcgcaca
     8461 gcgtccctcg gcgggaagcc cgagccggcg cgcgaacgcg acgtacttgg ccagctccgc
     8521 ctctgtgctc gccttcaagg cttcgatctc gtcgagcccc ttgaaccgtc ccgaatcgat
     8581 cgcgcccacg gagatgaaca cgacctgttt gaactgattc gggaagatac ggaagatgtt
     8641 gaggagcgag tgaatgccga gcccgccgaa tccgacgacc gtgagcacgg cggtctgggc
     8701 gttcttcttc aacggcgatt ccgtcggctc gcccgtgggg agcggcacgg tgaggaggct
     8761 ctcgtccagc ctctggagcg tcgccgcggt gttcacgtag tgcttccgca ccgacacgca
     8821 gaccgcgcag atcgccccgg tcgcgacgag cgtggaccac ccgccttcca tgaacttgaa
     8881 gctgacggtg ccgacgagga tcgccgcggt catgaagaat ccgatcccgt tgatcgcgag
     8941 cgcgcgcttc cacgtgtgcc cctcgcgccg cgcgatgaac gagtcgcgca ccatgccgag
     9001 ctgggcgagg gtgaacgtga ggaacacgtt gatcgagtag aggaccacga gcacgtggat
     9061 ctcgccctgc gtcagcatga gcacgaaggc ggccgagagc gccatgaaga ggacgccgtt
     9121 cgaagtgacc aggcgctccg agaggtttcc gaagcgcgac ggcacccacc ggtcgaacgc
     9181 catgtagacc aggacgcgcg ggccgccgag gaagcccgtc tgcgcggcga cgaagaggag
     9241 cgcgcccgcc gagatcagcg tgatcgcgac cagcacggcc cccaccatgc tcccgggcgc
     9301 gaagacgtgg ccggcgagcg cggaccagag aaccgcgttc agcgtcttgc cgggcacggg
     9361 ctgcactcgg tacagcaggt agttcacgag aatccccgcc gcgatcaccg cgagcgagaa
     9421 ggccatgtac cgcatcgtgg tcttcgcggt ctccaccttc ggctcgcgga gcatcggaac
     9481 gccgttgctc acggcctcga tcccggtgta cgtaccgccg cccagcgtga aggcgcggaa
     9541 cagggtcagg aggagcggga tccatccggt cgtcgcgatc gcggccgacg actgctcgtg
     9601 gaggtgccgc acctcgcccg gaatctggtg aatgttcagg acgatccccg agatgatcgc
     9661 gaccgcgtgc gcgagcacga acacgaggaa gatcggcgag atgatcttga ccgactcgcg
     9721 aacgccgcga aggttgagga gcgtcaggag cccgaggagg ccgaaggtga acgggagcga
     9781 cgcgaaatgg agccccggtg ggagcaggct gaagagcgac tcggagcagg ccgcgagcga
     9841 gatcgccacc gtgagcacgt agtcgacgag gagcgcgctt ccggagacga gccctgcggt
     9901 gggcccgagg agcttcgtcg cgacgacgta gccgccgccg ccggccggga agcgctcgat
     9961 gatgcgcatg tagctcgcgg cgatcacgac gaccgtgatc gcggtcagga tggcgagcgg
    10021 gatcgcgagg aagtcgtttc cgtggagcgc gaggaacgac gcttcgggtc cgtacgcgct
    10081 cgacgagagc ccgtccgcgc cgaggccgac ccaggccagg aacgcggcga gcgagatctt
    10141 gtggaagagg tggggatcgg tcaggcgccg cgcgggcccg atgacgacct cgaatgcccg
    10201 ccggatggcg cgaagccacc gcggctcgag gttgtcgggc gggggcgcgt ccgggggaag
    10261 ctccggaagc tctggctgcg cgggctcttc ctgcgtgggc gaggccattc gaggggggga
    10321 ctatacgccc cccgattttc ctccgcaaat ggaccgcccc ggcggtcatc ccgccggggc
    10381 ggcatctcaa gcgtcaaacg accttagacg gccgtcacgt tggcggcttg cttgcccttc
    10441 gggccctcga cgatctcgaa ctcgaccgcc tgaccctcgg ccagggtctt gaatccatca
    10501 ccctggatag cggagtagtg gacgaacacg tcctctccac cggcctgctg gatgaagccg
    10561 aatcccttgg cttcgttgaa ccacttcacg gttccgcgct tacgcatggt tcgaatcacc
    10621 tcccctcgat agcgtgctgg tatcgcttcc cttccacgcc gttgttccgg ggaatgcggc
    10681 cagaacccac gccttcggca gggccgagga tggacggacg cacccggggt cttttcgtga
    10741 tcgaggagat gtagggaggt gcttcagcgt tctgacaccg agtcgtgctt tgaaccttgc
    10801 atcgtgccag cgccgagctt gtgactgcga ttgcgaggca acaccattgc aggtttccaa
    10861 agctttccat gcgtggccgg caaaccctcg ccgacctaaa catgcggtca cggtagccag
    10921 ggggtgcctg ggtgtcaaag ggaaagttgc cccgccttga ccgggggcag ccttggccgt
    10981 accgtcgcgg cggcgtttaa ccccttgcgc gcgacggagg tcggatgacc gcttcgattc
    11041 gtgaagtgct cctcgggatg gcgtgcggcg cgctcctcgc cgcgccggct aaggccgcgg
    11101 actcggccgg cgcgcgcgtc gggaccgccg tgagccgggg cgcgcccgag gtcgtgatct
    11161 acaacgacga ccgcgcgctg gtgcgccagc cggtcgacct tcccgccggc gcgtcgcggg
    11221 tcgagatggg cggacttccg gcccgcatcg actccacgtc gatccggctc gacggcgtgc
    11281 gcgtgctccg ccaggcatac cagtacgacg tgtgggacgc cgatctcgtc ttccggcggt
    11341 gtcttgggga ttcgatcttc taccgctacg gcggccggag ataccgcgga gtgctcgccg
    11401 gaatcgacgg aaacgagctc ttcatcctcc gcgcggattc gaccgacgtg ctcacgatca
    11461 tgaatcgcca gcagctctcg gagatcgagc tgccgcgcgc ccggcggctt cgcaccacgc
    11521 ccacgctgga atgggagctc gagtcgggca aggcccgttc ggcgaatctc agctacgtca
    11581 ccgccgggct cgggtggacg gccgagtacg ccgcggtgct cgacaaggac gagcgcgggc
    11641 tcgagctcag cgggtgggcg atcatcgcga atcggtcggg caccgcattc cgggacgcga
    11701 aggtgatgct ggtggcgggc gaggtgaatc cgggaggcat gcttccggat cgggggctcg
    11761 ccgccgaggg cgccccggcc gcaacgccgt ccaccgcgcc ggagctcttc gcctaccgct
    11821 cctatccgct cgaccgcgcg ctctcgctcg acgatctcga gaccgcgcag gtcccgatcg
    11881 tcggtccggc acgcgtcgcg gcacggcgca cgtaccggtt cgacggcgcg cgggacggct
    11941 ccaaggtgcg ggtcgagctc gagttcacca acgacaaaac gtccgggctg ggtgcgccgc
    12001 ttcctgaagg gcgggtccgg atctacgccg ccggcgcggg cggaggccag gccctcgcgg
    12061 gagaagatcg aatccagcac accgcggcgg gcgagaaggt gcggctcctg agcggggtcg
    12121 cgttcgacct cgtcggcgag cgcgagcgtg tccgccacac ccgcgtgtcg cgcaacgtga
    12181 ccgaggacgc ctaccggatc gtcctccgga atcacggcgc caaggcggcg aaggtgacca
    12241 tcgaggagac gctctacgga aactgggaga tcacgcagaa gtcctcggac ttccgccgcc
    12301 ccgacgcgga taccgccgag ttcgatctcg acgtcccgtc gggcggtcaa gccgaggtca
    12361 cgtataccgt ccgctacacg ttctgagggc tccgggatga agctcctcgg cgcggccctc
    12421 ctcctggcgg tcgcgctgcc cagcgccggc agggcgaacg aagcgtcgcc gtcaccctac
    12481 tgggtgttct tccgggatcg gccggcggcg agcggcgccg ccgcgatcct cacgccgcgc
    12541 tcgctcgctc gacgggcgaa ggcagctcgc gcgcggggtg aggcgccacc ggcagccgtg
    12601 gacgatcgcg atcgcgaccc cgatgtcgcc tacgttcgcg ccgtgggaga cgccggtgcg
    12661 atcctccgca cgaaatcgcg atggctcaac gcggtcagcg tggatgcgga ctccgcggtg
    12721 ctcgaccgaa ttcgagcgct tccgttcgtg acgggggtcg atcgcgtcgc cgtgctcacg
    12781 ctccccgagg caagcgccga cgtcgccacg agtcccgagg tcgccacgag ccctgacaag
    12841 cccaccgacg ccgacgccgt cgagtacggc gactcctacc accagctcga gatgctcggc
    12901 gtgccggaag cgcatgccct gggggatcac ggccagggtg tcctcatctg cgtcctcgat
    12961 tcgggcttcg agcgctcgca cgaggtcttc gaccggctcg tcgtgcgcga cgagcgggat
    13021 ttcgtcttcg acgatcggga cacgggctac gaccccggcc aggacctgct cacgcaggcc
    13081 gaccacggca cctcggtcct ctccgtgctt gcgggctacg caccgggccg gctcatcggg
    13141 cccgcgtacg agtccgagtt cctcctcggg aagaccgagc ggatcggatc ggagcttcga
    13201 agcgaggaag accactggtg cgaggcggtc gagtgggcgg aggccgaagg ggcggacatc
    13261 gtgtcgagct cgctctcgta tccgctctgg taccggctcc aagaacggga cggtcgcacc
    13321 gcggagaact cgcgcgtcgc caacatcgcg ctcgagcggg gagtcctcgt cgtgaactcg
    13381 accgggaact acggcccggg gccgatgacc ttgagtccgc cgtccgacgc gcccggcgtg
    13441 ctcgcggtgg gatcggtgga cgcgaacgga aggctgagcc cgttcagctc gatcggcccc
    13501 acgtgggacc agcggatcaa gcccgacttg gtcgcgatgg ggagcgccgc gcgaatggcg
    13561 agggcgcgca cacgccacga ctacatgacc gcgaacggga cctcgttctc gacgccgctc
    13621 attgcgggtt gcgccgcgct cgtgctgagc gcgcatcccg actggtcgcc cgaagcggtg
    13681 tgggacgcgc tcgcgatgag cgcggatcgc acctcgcgtc ccgacccgag gtatggatgg
    13741 ggcgttccca acgtcctcga agcgatcctc tatcctcaga tcgagggacg gatcaccgat
    13801 gtgtccacgc caggcgtgcc cacggtgggg aagccggcga ccgtctcctg ggagcccgca
    13861 ccgggcgctc ccgcgccgag cgccgcggac acgctcgcgg cgccgagtga ttcgccgcgg
    13921 cgaggctcgg tgcgggcgga ttccaccggc gcctacgtga tccccaatct tccgtccggg
    13981 acctatcggc tcaccatcga ggcgcccggc tatttctccg tccagtcgga gccgctccaa
    14041 gttccacccg cgctcggcga cgtcgacttc gcgctgcgct atcgaggaga gtgagatgcc
    14101 cgagtatccg tattccaccc acctgaacat tctcaaggga ccgctcgaga cgatcgacgt
    14161 ggggcagctc gccgacgagt gcgaggagcg ctggtggaac cagactctgt gccaggtgaa
    14221 cgcctcggtc gtgcggctgg gcgtgatcca gggggagtac cactggcaca agcacgacca
    14281 cgacgacgag ttcttctatg tcgtcgaggg gacgcttctc ctcgatctcg aggggcgcac
    14341 catcacgctc gagccgcggc agggattcgt ggtgccgaag ggtgtggtac accgaacccg
    14401 cgcgccggag cgcacggtga ttctcatggt ggagaacgcg gggatcgttc ccacggggga
    14461 ctgacccggg cgcctacctc atcccgccca acccgcgcat cgatccctcc cagtggatgc
    14521 ggccgtcctt ccggaattcg tagcgcgggt tccgctggag gaagtcgacc gccgcgtcgc
    14581 gcagcatgac gccggttcgc tccggctcgc ccgccttggc gaagatcgtg tagccgtcgc
    14641 cgccgccgat gaggaagtcg atggtggcga cgcggtacac acgctccgaa tcgagcaccc
    14701 gtcctcccac cttgatgtcg gtggcgtggt cgccgcgaat ggtgaaggac acgcccgata
    14761 cttgagggaa gccgcccttt ccgagccggc gcgccccgaa gtcgaggagc tggcggagct
    14821 gccagcccgg catcatcacg accgccacct cgttgtcgaa cggcaccacg gagtagatgt
    14881 cgcccgcggt gacgcgacca cgcggcaggg gagcgcggat gccccccgag ttcacgatcc
    14941 cgacgtccgc gttcccgacg tcccgcgtca catcggcgac gaaattcccg agcggcgtct
    15001 cgccgtcgcg aaggccgctg gacgggacgg cgtccacggt ctcgaacacg gcgcgatcca
    15061 cgagcaccga catcgagtcg gcgagcggct tcagatacgc caggaccgat gcgtcttcgc
    15121 cgttggcggg attcacggga aggagcgatc cggcgtaccg cacgggcgtc cggcccttca
    15181 ggtagagcgc gatccgcccg aggtagacgc cccgcgatcc cgcctgcacg atgacggtgc
    15241 cgtcgtagcc gttcggcgtg gcgttgtgga ccaggatcgg cttccggagc gccgtgtggc
    15301 tgtggcctcc gacgatgagg tcgatccccg gcacgcgctc ggccagcttc cggtccacct
    15361 cgaccccaag gtgcgtcacg gcgatgacga cgtcggcctg gcgtctgagc tcgggaacga
    15421 gcctccgcgc cacggcgatg ggatcaccca cggcgacccc ggcgttcggc ctgaccgcca
    15481 cgatcgtcgt cacgtcggcg gtcacgagac cgaagagcgc gacccgaagc cccgacggga
    15541 gcgtcgcgat cgtgaagcgc tgggtcaggt accggagtcg cgctccatcc gggaccttca
    15601 cccgtccaac ccagcgcgct cccttccggt cctccgccga gacggagtcc cccagggccc
    15661 aggtgctctc ggccgcgccg aagaggttgg cggagaggag ggggaacctg gcctccgagg
    15721 cggcacgccg ccaggccgcg ggcccgtcat cgaggtcgtg gttgcccagg atcccggcgt
    15781 cgtagcccat ccgggtcatc agggtgtagt cgggccggcc gcggtagaag gtgaagtagg
    15841 gggtgccctg gaagacgtcc cccgcgtcga ggaggagcgt ccgcactcga tcgcccccgc
    15901 ggacccgccc gagaagggcg gcccgcgccg ccgcgccacc cgccggtggg ccccccttgg
    15961 ccgtgtaggg catcagctgg gcgtgggtgt cgttggtgtg gaagacgacc agcgaatcga
    16021 gaacgcttga ttccgcccgg ggagggggcg tagcttcctt cgcgtaggaa cacggaggcc
    16081 ccagagtcag ggccagggcc aggaccgtca ggcgccacat gtcgaaagtt cttgacacaa
    16141 gtcctacaat actatacctt tacgcacctg agtccgaacc ccgttttcgg agatccccgg
    16201 tagcaacgag tggaattcgg tgacctcgtc tcttcctctc cccacttccg tagccttctc
    16261 gcggttccct cgattgctcc agagttgccc ccggcgaatc tcgcccgtcg ggggagttac
    16321 gcgttccggt gagtgggtca cggaggtttc ctgatgcccg aggtcgacaa ggttttgaaa
    16381 tgccgcgatt gcggcgagga gttcacgttc acggctggtg agcaggcttt ctacaaggaa
    16441 cgtggcttcc agcacgaacc cactcgctgt cgcaggtgta gggacgaaaa gaagcgcgtc
    16501 gcaacgggcg cgccgagcgg tatgatggca tcggcggggt cgaacgcctc gtcgcgtgag
    16561 ttccacgatg ctgtctgcac ctcgtgcggc acgcccacgc aggtgccgtt ccgtcccacg
    16621 ccgggcaagc ccgtgtactg ccgtgactgc ttccagtccg tgcggtctcc gaaaggagtg
    16681 agcatgtagc aaccccggcg cggcgtcgcg acggtctccc cgaccatttc ggaccgaaag
    16741 gagggtatcc gcagttgaga caccgcggat cggtcaagtg gttcagcgaa gccaagggat
    16801 atggattcat ccgtgaggat tcaggagagg agttcttcgt acatcactcc gcgatcctgg
    16861 cggacggatt ccgaacactg aatgaaggcc aagtggtcga gttcgatccc acggagggtc
    16921 aaaggggaaa gcaggccgcc aacgtcgtca agatcgacgc ctgacgagcc caatccaagt
    16981 acctgccaac cccggcgggg tctcccgccg gggttcgtgt ttgtcccgat ccatcgccgt
    17041 ttgaggtaga gttacgccgg ttttcggtcg ccgatcttcc aggaggaccc agctcgacat
    17101 gcgcccgccg ttccggttga tcccgttctc gctcctcgcc ctcgtggccc ttgccgttcc
    17161 cgccctcgcc gcaccgaccc tcgttcgcac gctccccaac aagagcacgc tcgtcctgcg
    17221 cgagaaccga acgcgcccca tcgtctcgat ccaggcctgg atcaagagcg ggagccggga
    17281 cgagacgcga agcgagcggg gcgcggcggt cgtcctccag caggtcatgc tgcgcgccac
    17341 aaagaagcgc gtcgcggacg tcatggagaa ggacatcgag ctctacggcg ggtcctttgg
    17401 gaacgaggtg ggctacgact actcgatgtt cgagatcacg cttcccgccc gctacttcaa
    17461 ggcgggggcc gacatcctga gcgagatcgt gacgcagccg aggctcgaca cgcgcgacat
    17521 ccaggagtcg atcggcatcg cgcgcgatca ggccaagtac gttctccggc aggccgagct
    17581 ggccaccaac aatcccgttc gcgacgcgct ctggccggat acgccgctgg gatcaccgtc
    17641 caccgtgccg gagctcgagc tcgccgcggt gaccatgccg ctcgcggtgc gctactacaa
    17701 gacgcactac gtgtcggaga acctgatgct cgtggtcgtg ggcgacgtgg acccggccga
    17761 agtgccttcg gtcatggagc aagcgttcgc gtccatgccg cagggcaagg cgccgtcgca
    17821 tcccaaggtg aaggccaagg gcgcaccctc caccaagatc tcgttcgccg atccgccgcc
    17881 cgacgccggc gcggcgttcg cggtcggatt ccgcgcaccc gcttggggca cggccgacgc
    17941 ggtcgcgctc gacgttctcc tcgcggccct catcgacagc ccgcactcgc gaatcctcac
    18001 ccggccggtc gaggggagcg acgcgttcac gaatctcacg gcgcagaggt ccttcgacgt
    18061 ggacggcggc acgatctccc tgtcggggcg catcgatccc atgcgcgccc aggacgcgga
    18121 gggggcgctg atcgcgctcc tcgcgcaggc gcgcagcgcg cccatctcgc agagcgaggt
    18181 ggatgacgcg gtcgacgcgg tcgtgagtcg agacggcttc tacgcatcgg acatgaacgg
    18241 gctcgcccgc gcgacggccc tcgcgtatct ccgcgggaag cccggagcgg acgaggtcta
    18301 cttcgaccgg cttcgcacga tccgtcccga ggatctgacc ggcgtggcgg cccagtacct
    18361 ggatctcgac cacgcggcgg ttgtggagat gatgcctcgg agcgcggccg acagcctccg
    18421 cgcgaagagc ggcttcgagg ggcgcatcaa ggagaagctg ggcatcaacc aggcggcgta
    18481 cggcaaaggg cccaaggtca cgcagagctc gccgcaagac cgccagaagc gcatcgacgc
    18541 gccgctcgcg aagatcccct cgacggcgcc cgacatggga cgtgcgcggg tcgagaagtc
    18601 cacgctcgcg ggcgggctcc gggtcctgac gagtcaggat cggagcgctc cgacggtgtc
    18661 cgtcggcgtc tatctcgccg gcggcacgcg ctacgagaac gagaccaaca acggcgtcac
    18721 gtccctcttg cgcgagtcca tcctcacgtc ggccgatccc aaggccgatc acggcgccgc
    18781 ctaccgtcac tcgcttcccg agctggggcg cttccagccg taccaggaca aggacatgtg
    18841 gggcttctcg gtcacggtgc cgtcggacga gtggaaggaa gcggtgaccc ggctcggcac
    18901 gatgctctcg catcccgaga tcgacagcgt gaccgtggac gcgacgcgtc tctatgtcct
    18961 cgacgcgctc aacaagtgga gcgagaacga tcaggcccag cgctcctgga gcatcttccc
    19021 caccaagtac caggtgagcg gataccggct cccggcgctc gggacgcggt acaacctcat
    19081 gacgatgccc tacggcacca tcatggattg gtacaagacc ttcgtcgtgc gggggaatct
    19141 cgtcgtctcc gtcttcgggg acgtcacgcc gtccgaggtc ggacccgccg tggatcacgc
    19201 cttcgccgac ctgtcgagca agccgttcca gccgccgccc gtggcgaagg agccgccctt
    19261 cgaggacttc cgagagaagt gggatctggg agccgggacg gccagcacgg tgaacctcgc
    19321 cttcaatggc cctccggccc ggagccgcga catccccacg ttctacgtca tcaactcgct
    19381 cctgagcggg cctcgcggct ggttcgagac gtacatgaat tcgaacgaag cgctgttcaa
    19441 gggctcatcc tcgatcgtgt cccagagcat cgacgagtcg ccgctcatcg cgtcggtcgt
    19501 catcgcgggc ccgatggtcg aggaggacgg ggtgaagctg ctcttccggc agttcaagaa
    19561 ggtggccttc tacgagatga ccgccgagga ggcggatacg ctccgcttcg cgaagatcca
    19621 cgcggcggga acgtatctga gccttctcgg gacgaacacg tcgcgcgcct tccagtgggg
    19681 acgtgcggaa gtgttcgggc tcggcatgga atatccgctc acgcttccgg tgaagatcga
    19741 cgcggtcacc gccgaggacg tgcaggccgt cgggctcgcc tacttcgaga aggaccagtt
    19801 caaccgcaag ccctacgcca tcacggaaac aagacccggc ggctggtagc gtggcgagcg
    19861 aggccgtcgc ggtcatcgcg accgtccgga acgaagcggc ctccatcgac cgcctcctcg
    19921 attcgctcct ctcgggatcc cgcacgcccg acgagatcgt gatcgccgac ggcgggtcca
    19981 ccgacggcac cctcgaacgc ctgcgcgccc gcgcgggctc cgaagcgcgg atccgcgtgc
    20041 tcgtcgctcc cgggaatcga agcgtggggc ggaacgccgc cgtgcgcgcg gcgcggtcgg
    20101 aggtgatcgc ctgcaccgac gccggcgtcg acgtcgaccg cgagtggctc gaccggatca
    20161 cgcggccctt cgccaaccca gacgtggacg tggtcgccgg cttctaccag cccgccggcg
    20221 agacttcctt cgagcgcgcg gccggcgttg tgtcggcgcc gcggctcgag gagatcgacc
    20281 tcgagcgatt tcttccctcg acgcgttccg tcgcgttccg tcgcgccgcg ttcgagcgcg
    20341 tcggcgggtt cgacgaggcg ctgtggcaca acgaggacac gccgttcgcg ctcgcgctga
    20401 aggaggcggg ctgccagttc gcattcgcgc ccgacgccat cgttcgctgg cggccgcgcg
    20461 gcgatctgcg cgcgttcatg caccagcatc ggaggttcgg gaccggcgac ggcgaagcgc
    20521 gcgtgcagag ctggttctac gccacgatcg ccgtgaagta cgcgatggcc gtggtgctgt
    20581 tcgtggcggg attctggttg cgggcggcgt ggtggctctt gggcctgggc gttgcgctgt
    20641 tcatcgccac acaggctcgt cgcggcgaag ggcggttggg gttgtgggag cggctggtcg
    20701 gcgtgcccgc gttgaaggtg gcctacgatc tggcctacct ctccgggtac gtgaagggta
    20761 ggcttggacc acccgcctcg gcgcgtcccg cgcccgggcg ctcgtagagg aggtcgcgat
    20821 ggctgaggga acgcgggtct tccgtcgctc gagcaacagg atgatcgccg gcgtgtgcgg
    20881 cggcatcgcc gagtacttcg gctggcctcc ggatcgcgta cggatcgcct atgtcctctt
    20941 gtcgatcctc agcatcgcgt tccccgggat cctggtttac ctgctcctgt ggctcgtcat
    21001 gccggggccg gcggggacgt agtggggttc tgcgtcttct gcgagatcgt cgcgggccgg
    21061 atccccagct acaaggtctg ggaggaccgc gacttcatcg cgttcctgga catcaagccg
    21121 atccgcccgg ggcacatcct gctggtgcct cgagagcacc acgaagcggt gttcgatatg
    21181 ccgccggacc tgtatgaccg cacgtttcga gctgctcgcc gcttggcggc tccgctcggg
    21241 aaacacttca acgtgcctcg ggtcgggatc gtggtggaag gattcggcgt cgcccacgcc
    21301 cacgtgcatc tcgtgccgat ctcggaggcg gggcagctca atccggcgct ggcgagtgag
    21361 gcgtccggag aagacttgaa gcgcacgcgg gacgccttgg ctcccgcgct cgagcactaa
    21421 gcccccgccg cgatttcctc ctccggctcg tccggggtgt cctccggccg cggattgaag
    21481 atcgcggcca gctcgctccg gagctggccc tcgtcgaggt tccacttgag ctcgcccttg
    21541 tgggcgatcg agatggcgcc ggtctcctcg ctcaccacga agacgcaggc gtccgtctcc
    21601 tcggccaggc cgagcgcggc gcggtgccgc gtgccgagca ggggcgacaa ccccgcggtc
    21661 gcgctcaggg gcaggatgca tccagccgcc gagatctgat ctccgcggat caccacggcg
    21721 ccgtcgtgga gcggcgagtg cggcgtgaag atcgtctcca gcaattcctt cgtcaccgag
    21781 gcgtcgagct tggtgccggt ctcgatgtag tcccggagcc ccatgtcccg ctcgaccacg
    21841 atcaaggcgc cccggcgctc gcggctcatc tcctccacgg cgctcaggat ctccttcagc
    21901 acgcctcctt cgccgacgcg gatgagggcg cgcaagagcc ggttctggcc gatgtgggtg
    21961 agcaccgcgc ggagctcggg ctggaagagg atcacgaaca ggatcaccca gacggtcttc
    22021 aggctgccga tgatccagtt gagcgcgctc agccggaacc actgcgccac gatcgaggcg
    22081 atcacgatga acacgagccc gacgaacatc tgggccgcgc gcgtcccttt gatcatggcg
    22141 aagagcctgt agaacaggaa cgccacggcc aggacgtcga tcaggtcgac gaggaactgg
    22201 ggacgaatct cgatcacgcg cgcttctccg taagggctct cgccgtctcc accacggcgc
    22261 cttcactaag caaagcttcg gccagcgtca acgcgtcgcg ggtcgcccgg acgtcgtggg
    22321 tgcggaagat ccgcgcgccg agggcgtagg ccgcgaccgt cgcgccgagt ccggcggcga
    22381 ggcggtcgtc gacgggctcg tcggtcagcg cgccgaggaa cgatttccgc gacactccga
    22441 tgtacaaggg gcggcccaga tcggtcaagc ggggaagccc gcgcagcacg cccacgctgt
    22501 ggactggccg cttcgagaag ccgacgccgg gatccaaagc gatcgtttcc tccggaacgc
    22561 tacgcacgag cgcccgccgc atggccatgt agagctccca gcgtacttcg tccacgacgt
    22621 cgtcgtagtc cttcatctcg gcgtcgtgga acccggaggg tccgccccgg gagtgcatca
    22681 gcaccaaacc ggcccctgct tcggccacga cgtcggccag accgggctcg tacgcgaggg
    22741 cggtcacgtc gttgatcatg gcggcgcctt ccgcgagggc gcggcgggcc acttcggcgc
    22801 ggtaggtgtc gacggacaga gggacgctcc cggcccgcgc tcggagcgcc ttcaccacgg
    22861 ccccgatccg cgtccactcc tcctcggctc cgaccggctc gcccgcgccc ggacgcgtgc
    22921 tctggcctcc gacgtcgatc gcgtcggcgc cttcctcgat catgcgctcg gcatggcggg
    22981 cggcctcgcg aggctcaacg aagcgacccc cgtccgagaa ggaatcgggg gtcacgttca
    23041 ggatgcccac gacgcggggc gtgcgggtga ggtcccaccg gccgaaccgg tgggcgaaga
    23101 tcagcggagc acgcgccacc tcggcgcgct ccacaacgtc gttctggcgc acgatgtcac
    23161 gcgagccccg ggctttcggt gggatgcacg cgcggcggag ccttctcgcg cttcggctcc
    23221 tcgaccgggg ccgccgcggg cgtcggcctg ttgtcgccgc cgccgtcgcg ccggatcggc
    23281 tcgagcttct cgccgcgaag caccttctcg acttcctcgc cgtccaggac ctcgcgctcg
    23341 aggagcgcca gcgcgaggag gtggagcttg tcgaggttgt cggtgaggag ctggcgtgcc
    23401 cggttggaag ccccttccac caagccgcgc acttcctcat cgattgcgat ggccgtcttc
    23461 tcgctgaagt ccttgtgctg cgagatctcg cgcccgagga agacgaactc ctccttgctc
    23521 ccgagcgtga tcgggccgag ccgctcgctc atgccccatt cgcagaccat gcgccgcgcg
    23581 atgttcgtgg cgcgccggat gtcgtcggag gcgcccgtcg tgagctgatt gagcacgatc
    23641 tgctccgccg cgcggccgcc catcatgtgc gtgaggatct ggagccagta gtcgcgcgag
    23701 aacgagtgcc gctcgtcctg cggcaggtag gccgtgagac cgagcgcccg tccgcgcgga
    23761 atgatcgtga ccttgtgcac cttgtcgctg ccgggctgga gccacgccac gagcgcgtgt
    23821 cccgcctcgt ggtacgacgt gctccgcttc tcggcgtccg agatgacgag gctccggcgc
    23881 tcggtgccca tcatcacctt gtccttcgcg tcctcgaagt cgagcgtgtt gacgcgcttg
    23941 tgattccggc gcgccgcgag gagcgccgcc tcgttcacga ggttcgcgag atcggcgccc
    24001 accatgcccg gcgtgccgcg cgcgatcgtg ttcaggttga cgtcgtcgcc caccggtatg
    24061 ttgcgggtgt gcacgcgaag gatgccctct cgaccgcgca cgtccggcac gtccacgacg
    24121 atctgccggt cgaagcggcc gggccgcagc aaagccggat ccagcacgtc gggacggttc
    24181 gtggcggcga tcaggatcac gccgtcgttc gactcgaagc catccatctc gacgaggagc
    24241 tggttcagcg tctgctcgcg ctcgtcgtgc ccgccgccga ggcccgcgcc gcgatggcgg
    24301 ccgaccgcgt cgatctcgtc gatgaagatg atgcaggggg cgtttcgctt cccctggtcg
    24361 aagagatcgc gcacacgcga cgcgcccacg ccgacgaaca tctcgacgaa gtcggagccc
    24421 gagagcgaaa agaacggcac gcctgcctcg cccgcgaccg ccttggcgag gagcgtcttg
    24481 cccgtgcccg gaggaccgag caggagcgcg cccttcggga tgcgtccgcc gaggcgctgg
    24541 aatttcttcg gatcgcgcag gaactcgatg atctcctcca gctcctgctt cgcctcgtcg
    24601 gcgcccgcca cgtccttgaa cgtgaccttg gggctcgact cgagcgcgac cttcggacgg
    24661 ctcttcccga atttcatcgc gctcgcgccg cccgattgga tctggcggat catcaccatc
    24721 cagagcacga ggagcgcgac gaacgggatg tacccgagcg cgatgctcca ccagttcatg
    24781 ccctggggct tggagttgac catggcgttc ggatccttgg cgaggatcgc gcggccgatg
    24841 tccttgtcct cagccggcag gtagaccttg aagaagacgt aggagaccgg gcgcccctcc
    24901 acgtgcgtga gctggctctg cttcagctcg ccgacgacct cacgctcgat gatggtcgag
    24961 gacttgatgt tcgccaggtc gatctgctgg aggaactccg agtagggaat ctcgtgcgtc
    25021 ttcgcgcgca tctcggtgta gatctggacg aacacgagca cggcgagcac gaggatcacc
    25081 cagaggagcg cggtgcgaag tggcctggcc ggcccgaacg gcgtgggctt gcgctggggc
    25141 gggcgaggcg gcggcgcggg gcgtcgcgag ggacggccgt tggacggctt gttggaacgc
    25201 gcgtaggggc gcggatccca gttcacaggg gtcaattcgg tacctcggct tgggttcggg
    25261 gtcacgtaca gaggttcctt tcggttcatc ggcagacgag ccggaggtct caagctcttg
    25321 gatgttcccg gatcgcttcg gcttcgcgca tccggcggtc gagaacgatg cggccaccgt
    25381 cccggtacgc ccgcacctgg ccgggcagat caagggaccg cggtcgcgtc gtcgtcgcca
    25441 gggtgagaag ggactcgatg tgcgcccccg cgatctcacg gaggcttccg gtcagctcct
    25501 gcagggcgag acgaaacacg taagctctca acggtttagg gtactggacg agccgggtcg
    25561 cgtccagcgc gatctgaccc ggctcgctca agacgatcgc ttcggcgagc cgctcccggg
    25621 cgatcgcgac gaggatctcg tcggcgtcgc gcgcggccga cgcggtccga aggagcgcct
    25681 cgcgggccga tgggttgagg cgcgaacgga ccagcgggag cacgtccctg cggatgaaat
    25741 tcctcgcggg aagggatgtc tcgttggtgg aatccacccg atagttcacc ccgcggcgtc
    25801 gcaaatactc ctgaatttgc tcccgcgtca catcgaggag cgggcgggca atccgcgtgc
    25861 cccgcacctt ccctcgaacc ggcatcccgg acagtcctcg caagcccgcc ccgc
//
LOCUS       DASTKC010000215         7675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1123830, whole genome shotgun sequence.
ACCESSION   DASTKC010000215 DASTKC010000000
VERSION     DASTKC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123830"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFR25_09010"
     CDS             <1..586
                     /locus_tag="VFR25_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941233.1"
                     /translation="ASIGIEQAFAAHGMKLPALGRGKAGSGPRARAAAGRRAGAGRER
                     VAPSRALRALDPDRPGGAAAMNAALGLPGHARLKRTTLPGRLGSGVREQFIHRDWIRV
                     RHPAFRRRGGVLVRVLCGCTLTRGPSGDVEEATIAPHPHPTPAEMTRRVRRWLALGAI
                     DMGRDGTQSSRAHFKARTPFRVGRGGVLERVYFE"
     gene            complement(689..1423)
                     /locus_tag="VFR25_09015"
     CDS             complement(689..1423)
                     /locus_tag="VFR25_09015"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9941234.1"
                     /translation="MEKHEGLSITWLGHATVLYQSPRGKSVLVDAWLDHNPATPDSDK
                     DLGAVDLMLITHGHYDHFDDCLTIARRYGPDIVCNFEIGEYLGLRKIDKVNGINKGGS
                     VELHGIRVTMLHAVHTSSIREGDSFFPGGEACGFLIQFEDGTRVYHTGDTAVFGDMKL
                     HGEIYKPDVAVLPIGDLFTMAPPEAALAAKMIGAKTVVPIHHSTFPALHGTPAALKEA
                     LKGSGIEVVDLKPGGRTAIRERAAAR"
     gene            complement(1453..2268)
                     /gene="nth"
                     /locus_tag="VFR25_09020"
     CDS             complement(1453..2268)
                     /gene="nth"
                     /locus_tag="VFR25_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9941235.1"
                     /translation="MDVPLLLTRLRTAYPDAKCALDHRNPFELLIATILSAQCTDARV
                     NMVTPTLFARFPDAEAMSRASQEELEELVRTTGFFRNKSKSIRGASERLVGEFGGVVP
                     RSMDELLRLPGVARKTASVVLGVSYGVAEGVVVDTHVARVSRRLGLTRNGKVEKIEHD
                     LMKAIPREDWIEYSHLIIFHGRRVCDARKPKCPVCPVQDLCPSAKYFLAGNVPPWERR
                     MAAERGERRAARAVPTRTSGKARKVAAKSKRKRSTSVARTAARGRKAPRLKRS"
     gene            complement(2270..2938)
                     /locus_tag="VFR25_09025"
     CDS             complement(2270..2938)
                     /locus_tag="VFR25_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9941236.1"
                     /translation="MSVLEARPVFPLPNVVFFPKTILALHVFEPRYRLMMEESLRGDQ
                     TVCVVLLKPGWEGNYYGSPEVHEIGCVGRIAEHKLLPDGRYNVALHGEYKASIQSFDR
                     NEPYRIARVQRIQEDSSWVGAPGSAELANELLELFQKAGAARAAGFDLTGVFGPHMGT
                     DAILNTIAMYLNVDPAEKQRLLEMESLELRYRAVHDFLRDTSAVQDSIDRLRHLFPGE
                     PRRN"
     gene            2941..4185
                     /gene="queG"
                     /locus_tag="VFR25_09030"
     CDS             2941..4185
                     /gene="queG"
                     /locus_tag="VFR25_09030"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9941237.1"
                     /translation="MNATTANAGRSPAGRGAATGSAEAIDLAAAIREEAHRLGFDAVG
                     FARAEAHPHGERLRAWLESGSHGTMDWMAREPERRADPRAALASAHTVISVAASYYRG
                     DWPRGSATSGRETKDGAAWTGRIARYAWGRDYHKRLKKRTLHLGRAIRRLKPGVRFAL
                     YVDTGPMLDRAWAERAGIGWIGKNTNVIRQGAGSWCFLGEIITDLDLPPSRPAGNHCG
                     TCERCIPACPTGAITAPYQLDARRCISYLTIEHEGPIPIELRPAIGARIFGCDDCQEV
                     CPWNRFATPTALPEFAERPDQQTPELIPLLALDDRAFRERFAGTALRRAGRDRFVRNT
                     AVALGNVGDERAVPALAQALHGDPDPTVRGHAAWALGRIGGSAARDGLLRALDRERDP
                     KARSEIEYALSTDTDPSTTKEP"
     gene            4187..4960
                     /locus_tag="VFR25_09035"
     CDS             4187..4960
                     /locus_tag="VFR25_09035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9941238.1"
                     /translation="MKRTLALLTLLALAVFLPSGATAGEMITFPKGSGTNGGYLAVPK
                     GNGTHPAVVVVHEWWGISDWIKGRTDALAALGYVALAVDLYGGKVAKDNDEAHEYMSG
                     LVDEDAVASLRAGADYLRSRKDVRAQAIGTIGWCMGGKYAIRLAAADPGISACVMYYG
                     APITDADKIKEIQASVLGNFGGDDKGPSPDQVKTFETALRGAGKRVDFKIYPGAGHAF
                     ANSTNPFGTYKPDYAKDAWARTSRFFDKELKKASVPKSH"
     gene            4998..5429
                     /gene="mutT"
                     /locus_tag="VFR25_09040"
     CDS             4998..5429
                     /gene="mutT"
                     /locus_tag="VFR25_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HET9941239.1"
                     /translation="MRSDEPPRTVRRHEVAAGIIWDGARVLIARRQDGDHQGGRWEFP
                     GGKRHDRETIEECLKREMREEIGIDVEVGALWRALTHVYPDRTVTLYFHLCRARAGTP
                     RPIECAEIRWVDPTDLHALPFVEGDIQVLPDLVRDLVARAV"
     gene            5404..6264
                     /locus_tag="VFR25_09045"
     CDS             5404..6264
                     /locus_tag="VFR25_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9941240.1"
                     /translation="MISSPEPFEIEGADGGPLRGDLYASATNEPRAVLLCHGFRGYKD
                     WGFIPLLASTIASQDTPVAAFNMSGSGVATPDGAFTERERFRKNTYAAELDDIDRVAR
                     WLRQRLDASGPGFGLGLIGHSRGGAMALLYAASHPEVRTVVSLAAPSRIGVWPEVAFE
                     AWRDHRPHVVRDFRTRAELPLGPEIYGDIERNGARYDLRRAAASLAVPYLVVQGDRDR
                     SVPLEEGQTLASWGPSGTTELAIIKGAGHSFQAGDKIRRTPPQLLEMIERVSAWVRRT
                     MKPDAREARP"
     gene            complement(6269..6889)
                     /locus_tag="VFR25_09050"
     CDS             complement(6269..6889)
                     /locus_tag="VFR25_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9941241.1"
                     /translation="MRKLISATFLALTLAFASAASAAPATWSIDPSHSQVGFSIRHFF
                     SKVPGTFSKFTGSVVYDPQKPENSSVKADIDPASIDTKNEKRDNHLRSEDFFDVVKFP
                     ALTFVSTKVTADGENKLKVDGNLTMHGVTKLVTLEVTFLGAGPSRQGEQRSGFEATTS
                     INRKDFGILWNKTLDQGGTMLGDDVDIKINVEGVIRPDQPAPPAKS"
     gene            7046..>7675
                     /locus_tag="VFR25_09055"
     CDS             7046..>7675
                     /locus_tag="VFR25_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9941242.1"
                     /translation="MAETRDLIILGSGAAGLTAAIYAARAELHPLVVEGIQVGGQLTT
                     TTEVENYPGFRDAIMGPALMAEMKAQAERFGTEFLQADAEKVNLRARPFEIHAGGQVL
                     RARALIIATGASAKLLGIPSEGKLLGHGVSACATCDGFFFKQKRLFVVGGGDTAMEEA
                     TFLTKFASKVTIVHRRDTLRASKIMQERARANPKIDFIWNSDVVEIHDLD"
BASE COUNT         1222 a         2642 c         2596 g         1215 t
ORIGIN      
        1 cgcgtcgatc gggatcgagc aggcctttgc ggcgcacggg atgaagctgc cggcattggg
       61 gcgcggcaag gccggttcgg gaccccgggc gcgcgcggcc gccgggcgtc gagcgggcgc
      121 cggccgggag cgcgtcgcac cgtcccgggc gctccgtgcg ctcgatcccg accgccccgg
      181 cggcgccgcc gccatgaacg cggcgctggg cctacccggg catgcccgcc tgaagcgcac
      241 gacgctcccc ggccggctcg gaagcggagt gcgggagcag ttcatccatc gggattggat
      301 ccgcgtgcgg catcctgcat ttcgcaggcg gggtggcgtg ctcgtgaggg tgctctgcgg
      361 ctgcacgctt acacgaggcc cgtcgggcga cgtcgaggag gccacgatcg cgccgcatcc
      421 acatcctacc ccggcagaga tgacccgccg cgtgcgccgc tggcttgctc tcggcgccat
      481 cgacatgggc cgtgatggaa cacagtcgag ccgcgctcac ttcaaggcga ggacaccgtt
      541 tcgcgtgggg cgtggcggcg tgctggagcg cgtttacttc gagtagaact ggcccaaggc
      601 cagccgatgc cggacgagcg tgcggaaacc agcaacgtgc gacgtcgcaa gttcggaacg
      661 agcaatcact cgtcctggga cgagtcccct acctcgccgc tgcgcgctcc cggatcgcgg
      721 ttcgtccgcc cggcttcaga tccacgacct cgatgcccga gcccttgagc gcttccttca
      781 acgccgcggg cgttccatgg agcgcgggga acgtggagtg gtggatcggc accaccgtct
      841 tcgcgccgat catcttcgcg gcaagcgcgg cttccggcgg cgccatggtg aagagatctc
      901 caatcggcag gacggcgacg tccggcttgt agatctcgcc gtggagcttc atgtccccga
      961 agacggccgt gtcccccgtg tggtagacgc gcgtcccgtc ctcgaactgg atcaggaagc
     1021 cgcaggcttc gcctcccggg aagaacgagt ccccttcccg gatgctgctc gtgtgcaccg
     1081 cgtggagcat cgtgacgcgg atgccatgca gctcgaccga gcctcccttg ttgattccgt
     1141 tcaccttgtc gatcttacgc aaccccaggt attccccgat ctcgaagttg cagacgatgt
     1201 ccgggccgta gcgccgcgcg atcgtgagac agtcgtcgaa gtggtcgtag tgaccgtgcg
     1261 tgatgagcat gaggtccacc gcgccgagat ccttgtcgga atcgggcgtc gccggattgt
     1321 ggtcgagcca ggcgtcgacc aggaccgact tcccccgcgg cgattgatag agcacggtcg
     1381 cgtgaccgag ccacgtgatc gagagtcctt cgtgcttctc catgacaacc tccccggccg
     1441 cggcgcggcc gctcagcttc gtttgaggcg gggcgccttc cggccgcgtg cggccgtgcg
     1501 cgccacgctc gtgcttcgct tccgtttgga tttggcagcc accttgcgag ccttgccgct
     1561 cgttcgcgtg ggcacggccc gcgccgcccg ccgctctccc cgctccgccg ccatcctgcg
     1621 ctcccacggc gggacgttcc ccgcgaggaa gtacttcgcg ctcgggcaga gatcctggac
     1681 ggggcacacc ggacacttgg gcttccgcgc atcgcacacg cgccgcccgt ggaagatgat
     1741 caagtgcgag tattcgatcc agtcctcgcg cgggatcgcc ttcatgaggt cgtgctcgat
     1801 cttctcgacc ttgccgttcc gggtcaagcc gagccgccgc gacacgcgcg cgacgtgcgt
     1861 gtcgaccacg accccttccg ccacgccgta ggagacgccg agcaccacgc tcgccgtctt
     1921 tcgcgccacg ccgggaagtc gcaggagctc gtccatcgaa cgcggaacga cgccgccgaa
     1981 ttccccgacc agccgctccg aggccccgcg gatcgatttc gacttgtttc ggaagaaccc
     2041 ggtggtccgg accagctcct cgagctcctc ctgcgacgcg cggctcatcg cctccgcgtc
     2101 cgggaaccgg gcgaacaagg tcggcgtgac catgttcacc cgcgcgtccg tgcattgcgc
     2161 ggagagaatg gtggcgatca ggagctcgaa cggattccgg tgatccagcg cgcacttcgc
     2221 gtcgggatag gcggtgcgga gccttgtgag aagaagcgga acgtccacgt cagttccgcc
     2281 gaggctcgcc ggggaagaga tggcggaggc ggtcgatcga gtcctgcacg gcggaggtgt
     2341 cgcgcaggaa gtcgtgcacg gcccggtagc ggagctccag gctctccatc tcgagaagcc
     2401 gctgcttctc ggcaggatcg acgttcaggt acatggcgat ggtgttgagg atcgcgtcgg
     2461 tgcccatgtg cggcccgaag acgccggtca ggtcgaaccc cgcggcgcgc gccgcgccgg
     2521 ccttctggaa gagctcgagg agctcgttcg ccagctcggc cgacccgggg gcgccgaccc
     2581 agctcgagtc ctcctggatc cgctgcaccc gtgcgatccg gtagggctcg ttccgatcga
     2641 aggactggat ggacgccttg tactcgccgt ggagcgcgac gttgtagcgc ccgtcgggga
     2701 ggagcttgtg ctccgcgatc cgccccacgc atccgatctc gtgcacctcg ggcgagccgt
     2761 agtaattccc ttcccagccc ggcttcaaga ggaccacgca gacggtctgg tctccgcgca
     2821 gggattcctc catcatcaga cggtagcggg gctcgaagac gtgcagggcg aggatggttt
     2881 tcgggaagaa caccacgttg ggaagcggga agacaggacg tgcctccaga acgctcatcg
     2941 atgaatgcta ccacagcgaa cgcgggccgg agcccggcgg gcagaggagc cgcaacaggc
     3001 tccgccgagg cgatcgacct cgccgccgcg atccgcgagg aggcccaccg cctcggcttc
     3061 gacgcggtcg gattcgcgcg cgccgaggcg cacccgcacg gcgaacggct ccgcgcctgg
     3121 ctcgagtcgg gcagccacgg cacgatggac tggatggcgc gcgagcccga gcggcgcgcc
     3181 gatccgcgcg cggcgctcgc ctccgcgcac acggtgatct cggtggcggc ctcgtactac
     3241 cgtggtgact ggccgcgcgg ctccgcgacc tccggtcggg aaaccaagga cggcgccgcc
     3301 tggaccggcc gcatcgcccg ttacgcctgg ggccgcgatt accacaagcg cctcaagaag
     3361 cggaccctcc acctcgggcg agcgatccgt cgcttgaagc ccggcgtccg cttcgcgctc
     3421 tacgtcgaca ccggaccgat gctcgaccgc gcgtgggcgg agcgcgcggg gatcggatgg
     3481 atcggcaaga acacgaacgt catccgccag ggggcgggat cctggtgctt cctgggggag
     3541 atcatcacgg acctggacct cccgccgtcg cggccggccg gcaatcactg cgggacgtgc
     3601 gagcgctgca tccccgcgtg ccccaccggc gcgatcaccg ctccgtacca gctcgatgcg
     3661 cgccgatgca tcagctacct cacgatcgag cacgaagggc cgatcccgat cgagctccgc
     3721 cccgcgatcg gcgcgcggat cttcgggtgc gacgattgcc aggaggtctg cccctggaat
     3781 cgattcgcga cgcccacggc gctgcccgag ttcgcggagc ggcccgacca gcagacgccc
     3841 gagctgatcc cgctcctcgc cctcgacgac cgcgcattcc gcgagcgatt cgccggcacg
     3901 gccctgcgcc gcgcgggccg cgaccgcttc gtccggaaca ccgccgtcgc gctgggcaac
     3961 gtgggcgacg agcgcgcggt tccggcgctc gctcaggcgc tccacggcga tcccgatccg
     4021 accgtccgcg gccatgccgc gtgggcgctc gggcgcatcg gcgggagcgc cgcgcgcgat
     4081 ggccttctcc gcgcgctcga tcgcgagcgc gatcccaagg cgcgctccga gatcgagtat
     4141 gctctttcca cggacaccga tccctcgacc acgaaggagc catgacatga aacgcacgct
     4201 ggccttgctc accctgctgg cactcgccgt cttcctcccc tccggcgcga cggcgggcga
     4261 gatgatcacg ttcccgaagg gttccggaac gaacggtggc tatctcgcgg tccccaaggg
     4321 caacggcacg catccggcgg tcgtcgtcgt ccacgaatgg tggggcatca gcgactggat
     4381 caagggccgg accgacgccc tggccgcgct gggatacgtc gcgctcgcgg tcgatctcta
     4441 tggcggcaag gtcgcgaagg acaacgacga ggcgcacgaa tacatgagcg ggctcgtcga
     4501 tgaggatgcg gtcgcctcgc tccgcgcggg cgccgactac ctccgctcgc ggaaggacgt
     4561 ccgcgcgcaa gccatcggaa cgatcggctg gtgcatgggc gggaagtacg cgatccgcct
     4621 ggccgcggcc gatccgggca tcagcgcgtg cgtgatgtac tacggcgctc ccatcaccga
     4681 tgcggacaag atcaaggaga tccaggcctc ggtgctcggc aatttcggcg gagacgacaa
     4741 gggaccgagc cccgaccagg tgaagacctt cgagaccgcg cttcgggggg ccggcaagag
     4801 ggtggacttc aagatctacc cgggcgcggg gcatgcattc gccaactcca cgaatccctt
     4861 cggcacctac aagcccgact atgcgaagga cgcctgggcc cgcacgagcc ggttcttcga
     4921 caaggagctg aagaaggcat cggtccccaa gagccactga tgctgccgcg tccatcgagc
     4981 cgccggaggt ccggccgatg aggtccgacg agccaccgcg gacggtgcgc cgtcacgagg
     5041 tggcggccgg catcatctgg gacggcgcgc gcgtcctgat cgcgcggcgg caggacgggg
     5101 atcaccaggg aggacggtgg gagttccccg ggggcaagcg ccacgatcgc gagacgatcg
     5161 aggaatgctt gaagcgcgag atgcgggagg agatcgggat cgacgtcgag gtgggggcgc
     5221 tctggcgcgc gctcacacac gtctatccgg atcggaccgt gaccctctac ttccatctct
     5281 gccgcgcgcg cgccggaacg ccccgtccga tcgagtgcgc cgagatccgg tgggtcgatc
     5341 ccacggatct ccacgccctt ccgttcgtcg agggagacat ccaggtcctg cccgacctcg
     5401 ttcgtgatct cgtcgcccga gccgtttgag atcgagggag cggacggcgg tccgctccgc
     5461 ggcgatctct acgcctccgc aacgaacgag ccacgcgccg ttctcctctg ccacggattt
     5521 cgcggctaca aggactgggg cttcatcccc ctcctcgcct ccacgatcgc ctcgcaggat
     5581 acgcccgtcg ccgctttcaa catgtccggc tccggcgtcg caaccccgga cggcgccttc
     5641 accgagcgcg agcggttccg aaagaacacg tacgcggcgg agctcgacga catcgaccgc
     5701 gtggcgcgct ggctgcggca aagactcgat gcaagcgggc caggcttcgg ccttggtctt
     5761 atcggtcaca gccggggcgg cgcgatggcg ctcctctacg ccgcctcgca tcccgaggtc
     5821 cgcacggtcg tgtcgctcgc ggcgccgtcc cggatcggcg tctggcccga ggtcgcgttc
     5881 gaagcctggc gcgaccacag gccccacgtc gttcgcgatt tccgcacgcg cgccgagctt
     5941 ccgctaggac cggagatcta cggcgacatc gagcgaaacg gcgcgcggta cgacctccgt
     6001 cgcgccgccg cgtcgcttgc ggttccctat ctcgtcgtcc agggagatcg cgaccgctcg
     6061 gtgccgctcg aggaaggaca gacgctcgcg tcgtggggac cgtccgggac gacggagctg
     6121 gcgatcatca aaggagcggg gcattcgttt caggcgggag acaagatccg ccgcaccccg
     6181 ccgcagctcc tcgagatgat cgagcgcgtg tcggcctggg tgcggcggac gatgaagccg
     6241 gacgcgcgcg aggcgcgccc gtagagcgtt acgacttcgc cggcggggcc ggctgatccg
     6301 gacggatcac gccttccacg ttgatcttga tgtcgacgtc gtcgccgagc atcgtgcccc
     6361 cctggtcgag cgtcttgttc cagagaattc cgaagtcctt ccggttgatc gatgtggtcg
     6421 cctcgaaacc ggagcgctgc tcgccctggc gggacggccc cgcgcccagg aacgtgacct
     6481 cgagcgtcac aagcttcgtc acgccatgca tcgtgagatt gccgtccacc ttcagcttgt
     6541 tctcgccgtc tgccgtcacc ttggtgctca cgaacgtgag cgccgggaac ttgacgacat
     6601 cgaagaaatc ctcggagcga aggtggttgt cccgcttctc gttcttcgtg tcgatgctgg
     6661 cgggatcgat gtccgccttc acgctcgagt tctcgggctt ctgcggatcg tagacgaccg
     6721 agccggtgaa tttgctgaac gtgcccggca ccttgctgaa gaagtgccgg atggaaaagc
     6781 ctacctgcga gtggctcggg tcgatggacc aggtagccgg ggcggccgaa gcggcggacg
     6841 cgaaggccag cgtgagggcc aggaatgttg ccgaaatgag cttcctcatg aacctgctcc
     6901 tccttggggg ggcgggcggc cttgaagcgg gaacgccgtt tgcctactat ccgatgaact
     6961 cacacttgga tgcggcgact cgggccccgg tttcgacctc ctcgacggcc catggcatct
     7021 aacccgtgga gaggaccgat gttccatggc tgagacccga gacctcatca ttctaggctc
     7081 cggcgccgcc gggctcacgg ccgcaatcta tgcggcccgg gcggagctgc atccacttgt
     7141 ggtggaagga attcaggtgg gcggccagct caccacgacc accgaggtcg agaactaccc
     7201 gggcttccgc gacgcgatca tggggcccgc cttgatggcc gagatgaagg cccaggccga
     7261 gcgcttcggg accgaattcc tccaggccga cgccgaaaag gtcaacctgc gcgcccggcc
     7321 gttcgagatc cacgccggcg gccaggtcct gagagcccgg gcgctcatca tcgcgaccgg
     7381 agcctccgcc aagcttctcg gaatcccgag cgaggggaag ctcctggggc acggcgtgtc
     7441 ggcgtgcgcc acgtgcgacg gcttcttctt caagcagaag cgcctgttcg tggtgggcgg
     7501 cggcgacacg gcgatggaag aggcgacgtt cctcaccaag ttcgcgagca aggtcacgat
     7561 cgtccatcgg cgcgacacgc tccgcgcgtc caagatcatg caggagcggg cgcgcgccaa
     7621 tcccaagatc gacttcatct ggaattccga cgtggtcgag atccacgacc tggac
//
LOCUS       DASTKC010000216         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1124298, whole genome shotgun sequence.
ACCESSION   DASTKC010000216 DASTKC010000000
VERSION     DASTKC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124298"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="VFR25_09060"
     CDS             complement(<1..1916)
                     /locus_tag="VFR25_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941243.1"
                     /translation="MRYPRRRWAVITIFAAGAAIAGLLTASDNPAEHVLSIVFIASAI
                     IAIFLLYDSLLRAKRRLEKLGRAHLMYEAAQRLSGTLVTDEIYDGLRELAARAMPIDG
                     MIVSSYDPSSCTARCVYGWAGGAPFDAAALPPITIDLAKSGGMQTEVIRTGEGRLYQD
                     VEKRVKNSGGRYYDVGKGGTVRDLNRPGAAPPGAKCAVMVPIKLDGSVVGVVQVMSDR
                     VNAYTKEHLDTLRSLVAPMAVALQNADLYARANREIVERVRVERALKRSEEMLREADR
                     RKDEFLATLAHELRNPLAPIRNAVALLPDKLPYCQEVIERQVGHMGRLLDDLLDVSRV
                     SAGTLAIRKERIRVAEVIRHAAESSHPMIVAGGHHLSMKIAGEPILLDADSTRIAQVI
                     SNLLNNAARYSEPGSKIKLTADRDGDDAVIRVKDNGVGIRAEMLPRVFEPFFQIDRSL
                     DRSQGGLGIGLTLVKQIVEIHGGAVTAASDGLGKGCEFTVRLPVARGAVGPASGRPDD
                     EVEAGMTTPRSRILVVDDLRDSADSLAMMLRAKGHDVRVAYDGYEAVEIALQYRPDVV
                     LLDIGMPRLNGYEAARTIRQQAGGDSPLLIAVTGWGHDENRLRTKEAGFDHHLVKPVE
                     PAVLTKLLAERWASR"
     gene            complement(2385..2789)
                     /locus_tag="VFR25_09065"
     CDS             complement(2385..2789)
                     /locus_tag="VFR25_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941244.1"
                     /translation="MKGLRSLVLLAVFALLTVSGADAAGKHVTATLSPVNGSGISGTV
                     ELTALAKGTQIVVRAEGLTPGTQYVSLYYDNGTCELEPYSQEDVIGEYTGNPAGRGVA
                     TGKADDDLDEINSVSVRLKSDFSLQACAAVTH"
     gene            complement(2965..3336)
                     /locus_tag="VFR25_09070"
     CDS             complement(2965..3336)
                     /locus_tag="VFR25_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941245.1"
                     /translation="MDDFALPRALVLKAFTPEPGPTVQLEAGDPVRVGEESSVYPGWI
                     RITAPNGSKTWAPIGYVRGEPGRAGRLLVDYDSTELAVKERDRVTVSLEYKGWGWVRT
                     EDQREGWVPLEILGPYSFQML"
BASE COUNT          522 a         1193 c         1205 g          604 t
ORIGIN      
        1 cgcgaggccc agcgctccgc gagcagcttc gtcaagaccg cgggctcgac cggctttacc
       61 agatgatgat cgaagccggc ctcctttgtc cgcaagcgat tctcgtcgtg cccccagccg
      121 gtcacggcga tcagcaaggg ggagtcgccg ccggcctgct gccggatcgt gcgagcagcc
      181 tcgtatccgt tgaggcgcgg catcccgatg tcgaggagga cgacgtcggg gcggtattgc
      241 agcgcgatct cgacggcctc gtatccgtcg tacgcgactc tcacatcgtg gcccttcgcg
      301 cgcaacatca tcgcgaggct gtccgcgctg tccctcagat cgtccaccac cagaatcctg
      361 cttcgcggcg tcgtcatccc tgcctccacc tcgtcatccg gccgccctga tgcgggaccc
      421 accgcgcccc gcgccacggg cagtcgcacc gtgaactcgc accccttccc caatccatcg
      481 ctcgcggccg tcaccgcgcc gccgtggatc tccacgatct gcttcaccag cgtgaggccg
      541 atcccgaggc cgccctgcga gcggtcgagg gaacggtcaa tctggaagaa cggctcgaag
      601 acgcgcggca gcatctccgc gcggatcccc accccgttgt ccttcacccg gatcaccgca
      661 tcgtcgccgt cgcgatccgc cgtgagcttg atcttgctcc cgggctcgct gtagcgcgcc
      721 gcgttgttca ggagattcga gatcacctgc gcgatgcggg tcgagtccgc gtcgaggagg
      781 atcggttccc cggcgatctt catcgagagg tgatgcccgc ccgcgacgat catgggatgg
      841 ctcgactccg cggcatggcg gatcacttcg gccacgcgga tgcgctcctt ccggatcgcc
      901 agcgtgcccg cgctcacgcg gctcacgtcg agcagatcgt cgaggaggcg ccccatgtgg
      961 cccacctgcc gctcgatcac ttcctggcag tacggaagct tgtccgggag gagcgcgacc
     1021 gcgttgcgaa tcggcgcgag tggattccgc agctcgtggg cgagcgtcgc gaggaactcg
     1081 tccttgcggc ggtcggcctc gcgcagcatc tcctcgctcc gcttgagcgc ccgctcgacg
     1141 cgcacgcgct ccacgatctc gcggttcgcg cgagcgtaga gatcggcgtt ctggagcgcc
     1201 acagccatgg gggcgacgag actccggagt gtgtcgagat gctccttcgt gtacgcgttg
     1261 accctgtcgc tcatcacctg caccacgccg acgacggagc catcgagctt gatcgggacc
     1321 atcacggcgc acttcgctcc gggaggagcg gcgccggggc ggttcagatc gcgcaccgtg
     1381 ccgcccttgc cgacgtcgta atagcgccct cccgaattct tcacgcgctt ctcgacgtcc
     1441 tggtagagcc gcccttcccc ggtgcggatg acttcggtct gcatgccgcc cgacttcgcg
     1501 agatcgatcg tgatcggcgg gagcgccgcg gcgtcgaacg gcgcgccgcc agcccagccg
     1561 tacacgcacc gcgcggtgca gctcgacggg tcgtacgacg acacgatcat cccgtcgatc
     1621 ggcatcgcgc gcgcggccag ctcgcggagt ccgtcgtaga tctcgtccgt gacgagcgtg
     1681 ccggagaggc gctgcgccgc ctcgtacatg aggtgcgccc gcccgagctt ctcgagcctg
     1741 cgcttcgcgc ggaggagcga gtcgtagagg aggaagatcg cgatgatggc cgaggcgatg
     1801 aacacgatgg agagcacgtg ctcggcggga ttgtcgctcg ctgtgagaag gccggcgatc
     1861 gcggctcccg cagcgaagat cgtgatcacg gcccagcggc gtcgtggata ccgcatagga
     1921 acgtcggtcg gcgcgagcgg gctttggccg ttgcggtcgg gcacaacgaa ctccgaattc
     1981 gtcgagagat cggtttttag cgagcaggtc tcggggtcgg cttgcccacg atcagtgtcc
     2041 cgagatgggg ggagtatacc cgcgtagaaa tggggtccaa gaatttttgt ggaaggtgtg
     2101 gataattttt gggtctaggc ccttaggggg ccgaacccgc gactgtggct ccgtttaggg
     2161 gcttggatgg ggtccaaaat accgtgggtt tggctccggt tcttcgtgcg gtcgggaggg
     2221 gtgctccgaa atgtcgtggc gggtgaaacc gccgcggagg catcggctct cacgatgccc
     2281 ctcgcgacgt ggatcgccgc tcgaggggag cggtttgcgg ctcggcggcc cggcgtggcg
     2341 gcgtggcggc tcggcgacgc ggggaccgcc ggccccgacc gcggctagtg ggtgaccgcc
     2401 gcgcaggcct ggagcgagaa gtcgctcttc agccgcacgg agaccgagtt gatctcgtcg
     2461 agatcgtcgt cggccttgcc ggtcgcgact cctcggcccg cggggttgcc cgtgtactcc
     2521 ccgatcacgt cctcctggct gtagggctcc agctcgcacg taccgttgtc gtagtagagc
     2581 gagacgtact gcgtgccggg cgtcaatccc tcggcccgga cgacgatctg cgtccctttc
     2641 gccagcgccg tgagctcgac cgttccgctg atccccgacc cgttgaccgg gctcagtgtg
     2701 gcggtgacgt gcttgccagc cgcgtcggcc cccgacaccg tgagtaacgc gaacaccgct
     2761 agaagaacga gggacctgag tcctttcatg gcgtctctcc ctgtggagtg tggactctgg
     2821 ccttccaagc cgggacgatc ccggccccca tgggtaagtc tgcgcctcga gggcggcgtt
     2881 acagggggaa tgacggcggc ggcgggcggg cggagggggg cggagggcgg agggcggccg
     2941 ggctgcggcg cggcccgtcg ggacctacaa catctggaac gaatacgggc cgaggatctc
     3001 gagcggcacc cagccctcgc gctggtcctc ggtgcgaacc catccccagc ccttgtattc
     3061 gagggagacc gtgacgcggt ctcgctcctt caccgcgagc tcggtcgagt cgtagtcgac
     3121 gagcagacgg ccggctcggc cgggctcgcc gcggacgtag ccgatgggag cccacgtctt
     3181 gctcccattc ggcgccgtga tgcggatcca cccgggatag accgagctct cttcccccac
     3241 ccgcacggga tcccccgctt cgagctggac ggtagggccc ggctccgggg tgaacgcctt
     3301 gaggaccagg gcgcgaggga gggcgaagtc gtccatccca tgatcctaac gcttcgcggt
     3361 gagattgcga tgccctgggt ccaactggcc ccggggccag ttcccagggt gagaacagcg
     3421 gcgcccaact ggccccgggg ccagttgcct acttcccgtg gccgagcatc ttcatcacct
     3481 ccgccaggcg gacgagcgcg cgcgcgactc gcatgcgtgc ggcg
//
LOCUS       DASTKC010000217         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1125512, whole genome shotgun sequence.
ACCESSION   DASTKC010000217 DASTKC010000000
VERSION     DASTKC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125512"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VFR25_09075"
     CDS             complement(<1..1208)
                     /locus_tag="VFR25_09075"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET9941246.1"
                     /translation="MSRVTSARTVTVGGTPLDWSELDLIASGAPARLAQEARTRMEPS
                     HSLIQRAAHGSAPVYGVNTGFGALKSVRIPEEDVERLQANLVRSHAAGMGPELEPKAV
                     RLMLALRAHSLALGASGVRVELVERLLAMLEKDCLPVVPSRGSLGASGDLIPLAHLAL
                     GIIGEGEMRRNGSVEPAAVALADAGLGPIALQAKEGLALINGTQAATALGVLALLEAA
                     RVMRAAHAACALSIEALCGSVKPFDPRVHALRPHRGQHDSAAAIRGLLEGSGILASHA
                     SCGRVQDPYSFRCAPQVLGASLDALRFAREAIRTEVNSVTDNPLCFTDPDEVVSAGNF
                     HGQPVAQALDFLAIGMAEVGSIAERRSFLLLDAAMSGLPPFLSRSAGLGSGLMIVQYL
                     QASLVSENRML"
     gene            complement(1196..>2199)
                     /locus_tag="VFR25_09080"
     CDS             complement(1196..>2199)
                     /locus_tag="VFR25_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET9941247.1"
                     /translation="GGAFRPEASEAIDEVRARHAAWLEVEQAIRAVTNLEEKGAAFYV
                     DKDERGAPRWNKRPLEVGVPLRSQLFETSACTLLTSATLTPGSDFEPFLASIGLALDE
                     VETAALPSPFPLERQVRTAVLDGPDPTRPGFVEQLSSLIVDLASTTRRNVLVLLTSYQ
                     MLDQVASRVRAPLAATGVPLFKQTPGEAAASLAREFRETEGAVLLGAASFWEGVDFPG
                     AALEVLVIARLPFPVPTDPLVMARSESIEARGGDAFRELMLPEAVLRFRQGVGRLIRT
                     AEDRGAVVVVDSRIARAGYRARFLSTLPHPPLIDQSAEGVAAHVRQWLVREEASCPA"
BASE COUNT          338 a          810 c          781 g          270 t
ORIGIN      
        1 agcatccgat tctcgctcac gagcgacgcc tggaggtact ggacgatcat gagccccgac
       61 cccagccccg cgctacggct caggaacggg ggcagtccgc tcatggcggc gtcgaggagc
      121 aggaacgagc gccgctcggc gatcgatccg acctcggcca tcccgatcgc gaggaagtcg
      181 agcgcctgcg ccacgggctg tccgtggaag ttccccgccg agaccacctc gtcgggatcg
      241 gtgaagcaga gcgggttgtc ggtcacgctg ttcacttcgg tccggattgc ttcccgcgcg
      301 aagcggagcg cgtccagcga ggcgccgagc acctgcggcg cgcagcggaa cgaataggga
      361 tcctgcaccc ggccgcagct cgcgtgcgat gcgaggatgc cgctcccctc gaggagcccg
      421 cggatcgccg ccgccgagtc gtgctggccc cgatgcggac ggagcgcgtg gacgcgtgga
      481 tcgaagggct tcaccgagcc gcagagcgcc tcgatcgaga gcgcgcaggc cgcgtgcgcc
      541 gcgcgcatca cccgcgcggc ctcgaggagc gccagcacgc cgagcgcggt cgccgcctgc
      601 gtgccgttga tcagcgcgag cccttccttc gcctggagcg cgatcggacc gagccccgcg
      661 tccgcgagag cgaccgcggc gggctcgacc gagccgttcc gccgcatctc cccttccccg
      721 atgattccca gcgccaggtg cgcgagcgga atgagatcgc cgctcgcgcc cagcgatccg
      781 cggctgggca cgaccggcaa gcagtccttc tcgagcatgg cgaggagccg ctcgaccagc
      841 tccacgcgga cgccgctcgc gccgagcgcg agcgagtggg cgcgaagcgc gagcatgagc
      901 cgcaccgcct tgggctcgag ctccggcccc atgccggccg cgtggctacg cacgagattc
      961 gcctgcagcc gctcgacgtc ctcctccggg atccggaccg acttgagcgc gccgaatccc
     1021 gtgttcacgc cgtagaccgg cgccgagcca tgcgccgcgc gttgaatcaa cgagtgcgag
     1081 ggctccatcc gcgtccgcgc ctcctgggcg agccgggcgg gcgcacccga ggcgatgagg
     1141 tccagctcgc tccagtcgag cggcgtgccg ccgaccgtca ccgtccgagc ggaggtcacg
     1201 cgggacacga cgcctcctcc cggaccagcc attgccgcac gtgcgccgcc acgccctcag
     1261 ccgactggtc gatcaagggc ggatgcggca gcgtggagag gaagcgcgcg cggtagccgg
     1321 cgcgcgcgat gcgcgagtcg acgacaacca cggcgccgcg atcctccgcc gtccggataa
     1381 gccttccgac accctggcgg aagcggagca ccgcctccgg gagcatgagc tcgcggaacg
     1441 cgtcgccgcc gcgcgcctcg atcgactccg accgcgccat cacgagggga tcggtcggaa
     1501 ccgggaacgg gagccgcgcg atgacgagca cctcgagcgc ggcgcctgga aagtccacgc
     1561 cctcccagaa ggacgcggcg cccaggagca cggccccttc ggtctcgcgg aactcgcgcg
     1621 ccagggacgc cgcggcctcg ccgggcgtct gcttgaagag cggcacaccg gtcgcggcga
     1681 gcggagcgcg cacgcgtgaa gccacctgat cgagcatctg gtacgacgtg aggagcacga
     1741 gcacgttccg ccgcgtcgtg gacgccaggt cgacgatgag ggacgagagc tgctcgacga
     1801 agccggggcg cgtcgggtcg ggaccgtcga ggaccgccgt gcgcacctgg cgctcgagcg
     1861 ggaacgggct cggaagcgcc gccgtctcca cctcgtccag ggcgagaccg atcgacgcga
     1921 ggaacggctc gaagtccgat cccggcgtga gcgtcgcgga ggtgaggagc gtgcacgcgc
     1981 tcgtctcgaa gagctgcgac cggagcggca cacccacctc gagcggcctc ttgttccagc
     2041 gcggagcgcc gcgctcgtcc ttgtccacgt agaacgcagc ccccttttcc tcgaggttcg
     2101 tgaccgcgcg gatcgcctgc tccacctcga gccacgcggc gtggcgcgcg cggacctcgt
     2161 cgatcgcctc ggacgcttcg gggcggaacg cgcctcccg
//
LOCUS       DASTKC010000218         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_891386, whole genome shotgun sequence.
ACCESSION   DASTKC010000218 DASTKC010000000
VERSION     DASTKC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891386"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /gene="ftcD"
                     /locus_tag="VFR25_09085"
     CDS             complement(<1..1454)
                     /gene="ftcD"
                     /locus_tag="VFR25_09085"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET9941248.1"
                     /translation="MNELVECVPNFSEGRNKSLVQALVAEISRDRSIRLLDQEMNADH
                     HRSVVTFVGEPEAVLEAALRGVRKATELIDLRQHRGEHPRMGATDVLPFVPVGSTTLE
                     RCVALARQAGERIGQELGIPVYLYEAAATRPARQNLADVRRGEFEGIAATIGTDPERA
                     PDFGPRAVHPSAGAIAVGARLPLLAFNVNLGTRDVEVAKKIAKAIRFQSGGLRYVKAL
                     GFELTERGIVQVSMNLVNTWGTPVHRVFALIRDEAERYGVPIVGSEVVGLVCQDALLD
                     VAEHALRLENFSRQQVLENRLATPIGAAELGLPEFLGSVSSSEPTPGGGSVSALAGSL
                     ASALGTMVARLTIGKKRYAEHEEEMRRVERSLEGLQGELFDLVSEDARAYDAFVLARK
                     MSQRTPEEVASRAKAMAEAARNAALVPMRTAETCVRVLTELVTVATIGNENAASDAGV
                     AAWLARTGVEGGALNVAINLPELGEADRADLEQR"
     gene            complement(1544..2821)
                     /gene="hutI"
                     /locus_tag="VFR25_09090"
     CDS             complement(1544..2821)
                     /gene="hutI"
                     /locus_tag="VFR25_09090"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET9941249.1"
                     /translation="MKADLLVHSAAQLITAPPGTKPLVGADLDRPILIEDAAIAVAGE
                     RIAAIGTTREILAAYPEREAARVLDARGRLIAPGFIDPHTHLPFAGMREMEFDARARG
                     ESYESIAAKGGGIRASVRHLRSIDEAALADRVTARLGTLLRHGITTVEAKSGYGLSPD
                     SETKQLRALRAAQSRSPVEIVPTFLGAHDIPEDYRDRRAAYVDLLTGTMIPEVAKAGL
                     ARFCDVWCDVGIYTIEESRAILLAGKAHGLLPKLHADELGDAGAAALAAEVGAVSADH
                     LLFASDAGLLAMAQAGVIAVLLPGTAFTLGLPYARARWMVEHGLAVALATDFNPGSTM
                     SSSMSLALTLAVTQMKLSPAEAWMAATANAASAIGEGARIGRIQPGFQADFCLFDASD
                     YRHIPYHYGHEHLHAVVKRGRVALEGVEAWTCT"
     gene            complement(2865..>3664)
                     /gene="hutU"
                     /locus_tag="VFR25_09095"
     CDS             complement(2865..>3664)
                     /gene="hutU"
                     /locus_tag="VFR25_09095"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017541723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET9941250.1"
                     /translation="PAAYIERSVASMGTHVRAMRDFQKQGSLTFDYGNNIRGQARKAG
                     VTDAFEIPGFVPEFIRPLFCEGKGPFRWVALSGNPKDIAVTDRAVTQLFPENDALQRW
                     IRLAGERVAFQGLPSRICWLGMGERERFGEVLHRLVERGEVEAPIVIGRDHLDTGSVA
                     SPYRETEAMKDGSDAIADWPILNALLNAVSGASWVSVHHGGGVGIGNSIHAGVVVVAD
                     GTPAGLARLRRVLTNDPAMGVWRHADAGYPEAVAVAKKNRLPIPTQE"
BASE COUNT          560 a         1339 c         1219 g          546 t
ORIGIN      
        1 atacgctgct cgagatccgc gcggtccgcc tccccgagct cggggagatt gatcgccacg
       61 ttgagcgcgc ccccctcgac gccggtgcgc gcgagccagg ccgcgacgcc ggcgtcgctc
      121 gccgcgttct cgttgccgat cgtcgcgacg gtcacgagct ccgtgagcac gcgcacgcac
      181 gtctccgccg tgcgcatggg aacgagcgcc gcgttccgcg ccgcctcggc catggccttg
      241 gcgcgcgacg cgacctcttc cggcgtccgc tgcgacatct tcctcgccag gacgaacgcg
      301 tcgtacgcgc gggcgtcctc cgacacgaga tcgaagagct ccccctggag cccctcgagc
      361 gagcgctcca cgcggcgcat ctcctcctcg tgctcggcgt accgcttctt cccgatcgtg
      421 agccgggcga ccatcgtccc gagcgccgag gccagagagc ccgcgagcgc cgagacggag
      481 ccgcctcccg gcgtcggctc cgacgaggac accgagccca ggaactcggg cagaccgagc
      541 tccgccgcgc cgatgggcgt ggcgagccgg ttctcgagca cctgctggcg cgagaagttc
      601 tccagccgga gcgcatgctc cgcgacgtcg agcagagcgt cctggcacac gagccccacc
      661 acctcgcttc ccacgatcgg aacgccgtag cgctccgcct cgtcgcgaat gagcgcgaac
      721 acgcggtgga ccggagtccc ccacgtgttc acgaggttca tggacacctg cacgatgccg
      781 cgctccgtca gctcgaatcc gagcgccttc acatatcgga ggccccccga ctggaaccgg
      841 atggccttcg cgatcttctt cgccacttcc acgtcgcgcg tcccgagatt cacgttgaac
      901 gcgagcagag gaagcctcgc gccgacggcg atcgcgccgg ccgacggatg cacggcgcgc
      961 ggcccgaagt cgggcgcccg ctcgggatcg gtcccgatcg tcgccgcgat gccttcgaac
     1021 tcgccccggc gcacgtcggc caggttctgc cgcgccggac gcgtcgcggc ggcctcgtac
     1081 agatagaccg gaatgccgag ctcctgtccg atccgctcac ccgcctggcg cgccagagcc
     1141 acgcagcgct cgagcgtggt cgagccgacc ggcacgaacg gaagcacgtc cgtcgctccc
     1201 atgcgcggat gctcgccccg gtgctgcctc aggtcgatca actcggtcgc cttccggaca
     1261 cctctcagtg cagcctccag aacggcctcc ggctcgccca cgaatgtgac cacggaacgg
     1321 tggtgatccg cgttcatctc ctgatcgagc aatcggatcg accggtcacg cgaaatttcg
     1381 gcaaccaggg cctgaactag agacttattg cggccctcgc tgaagttggg gacgcactcc
     1441 accagctcgt tcatgggctc tcctcatggg ttggcgcgaa gcgttcgggg gaggtcgcac
     1501 cccgacgaaa gcggctttga atcagggtct tacgaacgaa acctcatgtg caagtccaag
     1561 cttcgacccc ctccagcgcg acccgcccgc gcttgaccac ggcatggagg tgctcgtgcc
     1621 cgtagtggta ggggatgtgg cggtagtccg acgcgtcgaa gaggcagaag tcggcctgaa
     1681 accctggctg gatccggcca atccgcgctc cttcgccgat ggcggacgcc gcgtttgccg
     1741 tggccgccat ccaggcctcc gccgggctca gcttcatctg cgtgacggcg agcgtgaggg
     1801 cgagcgacat cgaggacgac atcgtgctcc ccggattgaa gtcggtcgcg agagcgaccg
     1861 cgagcccgtg ctccaccatc caccgtgccc gggcgtacgg cagcccgagc gtgaacgccg
     1921 tgccgggaag caggaccgcg atcacgccgg cctgggccat cgcgaggagc cccgcatcgg
     1981 acgcgaagag aagatggtcc gcggacacag cgcccacctc cgcggccagc gcggcggcgc
     2041 ccgcgtcccc aagctcgtcc gcgtggagct tgggcaggag cccgtgggct ttccccgcca
     2101 ggaggatcgc ccgcgactcc tcgatcgtgt aaatcccaac gtcgcaccat acgtcgcaga
     2161 agcgggccag tcccgccttc gcgacctcgg gaatcatcgt gccggtcaag agatcgacat
     2221 aggcggctcg ccgatcgcgg tagtcctccg gaatgtcgtg cgcaccgagg aacgtgggca
     2281 cgatctcgac cggcgatctc gactgcgcgg cgcgaagcgc ccggagctgc ttcgtctcgc
     2341 tgtcgggcga gaggccgtag ccgctcttcg cctcgaccgt cgtgatccca tggcgcagga
     2401 gggtgccgag gcgcgcggtc acgcgatcgg ccagcgcggc ctcgtcgatc gagcggaggt
     2461 ggcgcacgct cgcgcggatg ccgcccccct tggcggcgat cgattcgtac gactccccgc
     2521 gggcgcgcgc gtcgaactcc atctcgcgca tgccggcgaa gggaaggtgc gtgtgagggt
     2581 cgataaagcc gggcgcgatc aggcgtccgc gcgcatcgag gacccgcgct gcctcgcgct
     2641 cgggataggc ggcgaggatc tcccgcgtcg taccgatcgc cgcgatccgc tccccggcca
     2701 ccgcgatggc cgcgtcctcg atcaggatcg gacggtcgag gtcggcgccg acgagaggct
     2761 tcgtcccagg gggcgcggtg atgagctgcg ccgcggagtg gacgaggaga tcagccttca
     2821 cgcggcctcg gtccggcacc ggcccgccgc gctcctgggt cgcttcattc ctgggtcgga
     2881 atcgggagac ggttcttctt cgcgaccgcc accgcctccg gatatccggc gtccgcgtgg
     2941 cgccacacgc ccatcgcggg atcgttcgtg agcacccgcc ggagccgcgc gaggcctgcg
     3001 ggcgtcccgt ccgcgaccac gaccacgccg gcgtgaatcg agtttccgat tcccacgccg
     3061 ccgccgtggt gcaccgagac ccacgacgcg ccggacaccg cattcaggag cgcgttcagg
     3121 atcggccagt ccgcgatcgc gtcgcttccg tccttcatgg cctcggtctc gcgatagggc
     3181 gacgcgaccg agcccgtgtc gagatggtcg cgtccgatca cgatcggcgc ctcgacttca
     3241 ccgcgctcga cgaggcgatg gagcacctcg ccgaatcgct cgcgctctcc catgccgagc
     3301 cagcagatgc gcgacgggag gccctggaac gcgacccgct ctccggcgag ccggatccag
     3361 cgctggagcg cgtcgttctc cgggaagagc tgggtcacgg cgcgatcggt cacggcgatg
     3421 tccttgggat tgcccgagag cgccacccag cggaatgggc ccttcccctc gcagaagagg
     3481 ggccggatga actccggcac gaagcccggg atctcgaacg catccgtgac gccggccttc
     3541 cgcgcctggc cccggatgtt gttcccgtag tcgaacgtga gcgagccctg cttctggaag
     3601 tcgcgcatcg cgcgcacgtg cgtccccatc gacgcgaccg agcgctcgat gtacgcggcc
     3661 ggat
//
LOCUS       DASTKC010000219         6862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1604725, whole genome shotgun sequence.
ACCESSION   DASTKC010000219 DASTKC010000000
VERSION     DASTKC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604725"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFR25_09100"
     CDS             complement(<1..590)
                     /locus_tag="VFR25_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941251.1"
                     /translation="MSASQIKITYHPPSGGGCFVDVVRNFRTPDEEKLIRIAEVTYPL
                     REAFSPPDSLMLQPIPARKHLWWLGPEEDIRVPCAITMDALRYYLKMSQLYGRGRPPN
                     AYPGPRPVSSFTYAADVSHQASFKRDRMVFRDVSVVELRLDWGSGSMGFHLDRTVLLD
                     GSGKVVAVFGDRPASVLLTQAGPAPLGESEPRIGYEA"
     gene            complement(840..1274)
                     /locus_tag="VFR25_09105"
     CDS             complement(840..1274)
                     /locus_tag="VFR25_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9941252.1"
                     /translation="MYKRILVPLEHSSYDETILDHVRALARLCGSSLVLVHVADGWVA
                     RNIQNLNLRESEEMREDRAYLTEAREDLEGDGFKVEALLAAGDPAQEIAAAAEREHCD
                     LIAMATHGHKGLGDIIHGTTANALRHLTEIPILMVRGVKAAK"
     gene            complement(1274..2635)
                     /locus_tag="VFR25_09110"
     CDS             complement(1274..2635)
                     /locus_tag="VFR25_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9941253.1"
                     /translation="MHLSAKKSEEPRADAGWRQPRVTPSLAEVYRSVPVPTGTWWRRA
                     LAFAGPGYLVAVGYMDPGNWATDLAGGSAFGYTLLSVILISNLMAILLQGLSSKLGIV
                     TGRDLAQACRDHYSRPVSIFLWIICELAIAACDLAEVIGSAIALNLLFHIPLPWGIAI
                     TALDVLLILYLQNKGFRLVEALVIALIGTIAACFLFEILIAKPSLSGIAVGFIPSGSI
                     LADQHKLYIAIGILGATVMPHNLYLHSSIVQTRRYEETPEGKRMAVKYAFIDSTIALS
                     FALFINAAILIVAAATFHTTGHSGVAEIQDAYRLLTPLLGVAGASAVFAFALLASGQN
                     STLTGTLAGQIVMEGFLNIRIRPWLRRLITRLIAIVPAAITAIWFGASGTAQLLILSQ
                     VILSLQLSFAVFPLVNFTSDKIKMGEFVNPVWVKVLAYTVAVVIASLNVWLLVQTFRG
                     GAG"
     gene            complement(2653..2985)
                     /locus_tag="VFR25_09115"
     CDS             complement(2653..2985)
                     /locus_tag="VFR25_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941254.1"
                     /translation="MASKEELLARRRELIARSDAYREDIARSAEVWRAPLGTVDRVVG
                     IARDIGEKAPWLASVAGAIWMVARGRDKRGRGFKAPGIFGTAQLAWNVARGILGVTST
                     FRRRSGRT"
     gene            complement(2986..3378)
                     /locus_tag="VFR25_09120"
     CDS             complement(2986..3378)
                     /locus_tag="VFR25_09120"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9941255.1"
                     /translation="MDDIERDDRPGIISSLKRTISSTLGLIETRLELLGNDLALAHAD
                     ITKLVILVASVVLCLQLGLLLGVIFLVLVVPESARALTVGIAAFLVLACSIGGVLYLR
                     RWLGSRTGFFEATIGEFKKDRDKLRGRT"
     gene            complement(3387..3710)
                     /locus_tag="VFR25_09125"
     CDS             complement(3387..3710)
                     /locus_tag="VFR25_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HET9941256.1"
                     /translation="MTESSSITPSAARDQLVADLKAVVSDAEELLRATKDAAGEKVSA
                     ARARAEATIGKARAKLSNLEDATLDRAKEAAKTADDYVHEHPWNAVGIAAAAGIVIGI
                     LLARR"
     gene            complement(3757..4326)
                     /locus_tag="VFR25_09130"
     CDS             complement(3757..4326)
                     /locus_tag="VFR25_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET9941257.1"
                     /translation="MLSSLIERYWWIPLLRGLAAILFGIVALTMPGITLASLILVFGV
                     WLLIDGVFTIFHAFSGRRETPRWWMMLLEGLLAAALGIFALRSPGMAALTLLFYIAVW
                     AIISGIIRIVLSFALRREIQGEWWMILGGFASVLFGLIMIVRPGAGALALLTIIGIWS
                     ILAGVALVMLAFKARKFGKALASARPQPA"
     gene            4435..5064
                     /gene="can"
                     /locus_tag="VFR25_09135"
     CDS             4435..5064
                     /gene="can"
                     /locus_tag="VFR25_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HET9941258.1"
                     /translation="MSDLEECFEANRQWAARMREREPEFFERLRHQQQPELLWIGCSD
                     SRLPPDRIIGKQPGEVFVHRNVANVVVHTDMNCLSVLQYGIEVLGVRHIILCGHYGCG
                     GVLAALQTQPLGLIDNWLRHIRDVHAAHRDELDAIADEGARADRLCELNVAAQVANVC
                     RTTIVQDAWRAGRPLTVHGWIYSVSDGLLRDLGLSVSKLEDMPSRFHMS"
     gene            5086..5676
                     /locus_tag="VFR25_09140"
     CDS             5086..5676
                     /locus_tag="VFR25_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9941259.1"
                     /translation="MRPFLTAVFVLASILVLTQPAQATWGVGANMGLTIHNPEGPGDD
                     VTMFGIPTQANALSSIRPGLRVSYTGGAKMTHEGFLDLSYDRQSSDGDHLSTLRLAGN
                     YQYNFTMSGSPRPYVTFGVGMFHEGFDTGGGDVGATAMTFGGGFGVGFPVSKGAGRIR
                     TEVRMDKLDDADDDGFPIIGEATVIQIGAGFDLWFK"
     gene            complement(5677..6813)
                     /locus_tag="VFR25_09145"
     CDS             complement(5677..6813)
                     /locus_tag="VFR25_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9941260.1"
                     /translation="MMHFSLSRCLAVAERDLRRFRRNSKLMVPMALMPIIYLVILGKS
                     MGGDLHNLPVAVVVQDHGAASAEVLNRLVTMAQGRELFQLSSEPDPSSAVRELRQGRY
                     KGVVIVPPGFSDRYARGEPAPLGVVVDNTDNTAANVIVQELRGAFLGVNTELRAVTSA
                     PVFPGVQVERVDVYGHKEFMQYLVPGVIALALFFVAMLAGGIILVDDRARGIHEGYFV
                     TPLSSLDLVLGLTISAVSLSMIIGTIVLVASILIARLPIIGGLPTILMTEAAVFLLGL
                     GLILFMFTLMARVSNPMTPRALFGILNVVTFFPSGALYPTESYPHWLNAISMAFPMRY
                     AVHALRNLLLKGVGFSAVAPDFAVMAAFAGIMLVLASTMFKRTL"
BASE COUNT         1217 a         2216 c         2330 g         1099 t
ORIGIN      
        1 gcttcataac caatgcgggg ctcgctctct cccaggggag cgggccctgc ttgcgtcagg
       61 aggaccgaag ccggccggtc accgaagacc gccacgacct taccgctccc gtccaggaga
      121 acggtgcggt ccaggtgaaa acccatcgag ccgctccccc agtccagccg aagctcgacc
      181 acgctcacgt cgcgaaatac catccggtcc cgcttgaacg acgcctggtg ggatacgtcg
      241 gctgcgtacg tgaagctgct cacgggcctg ggtccgggat acgcgttcgg tggccgaccc
      301 cgcccgtaga gctgggacat cttcagataa taacgaagcg catccatggt aatggcacag
      361 ggcacgcgaa tgtcctcctc cggacccagc caccagaggt gcttgcgagc aggaatgggc
      421 tggagcatga gtgagtccgg gggcgagaag gcttcgcgaa gcggataggt gacttcggcg
      481 attcggatga gcttctcttc gtccggtgtt cggaagtttc ggaccacgtc gacgaagcac
      541 ccgcccccgc tcggagggtg gtacgtgatc ttgatctggc tcgcgctcaa gctgtggatg
      601 atgggtctcc ccgtgcccgg ggccgtggga tcgggtattg gcgcgccggg atcgaggaaa
      661 attgctccgg tcgtgtcgac ggcatcgcca gggaatggtt cgctgagctg agcccaatcg
      721 gtggcgtagg ggaggtgcgt cttcggggcg gcttcgcgtc gtggggagca gcctgcgagc
      781 gtgagactca gccctagggc gagtccgaca gccaccggtc cttgcggtct gcccacggac
      841 tacttagcgg ccttcacgcc tcgcaccatc aggattggaa tctccgtgag gtgccggagc
      901 gcgttcgccg tcgtcccatg gatgatgtcc ccgagccctt tgtgtccatg ggtcgccatc
      961 gcgatgagat cgcagtgctc gcgctcggcg gcggcggcga tctcctgggc ggggtcgccg
     1021 gcggcgagga gcgcctccac cttgaagccg tccccttcga gatcctcgcg ggcttccgtc
     1081 aggtaggcgc gatcctcgcg catttcctcg gactcgcgca ggttcaggtt ctggatgttg
     1141 cgcgccaccc agccgtcggc cacgtgcacg agaacgagcg acgacccgca gagcctcgca
     1201 agcgcgcgca cgtggtcgag aatggtctcg tcgtacgacg agtgctcgag cgggaccagg
     1261 atgcgtttgt acatcagccg gctccgcctc ggaacgtctg taccaggagc cacacgttga
     1321 gcgaggcgat cacgacggcg accgtgtagg cgagcacctt gacccagacc ggattcacga
     1381 actcgcccat cttgatcttg tccgacgtga aattgacgag cgggaagacg gcgaacgaga
     1441 gctgaaggct caagatcacc tggctcagga tgaggagctg cgctgttccg ctcgcaccga
     1501 accagatggc ggtgatcgcc gcggggacga tcgcgatgag ccgcgtgatg aggcgtcgga
     1561 gccaggggcg gatccggatg ttgagaaatc cctccatcac gatctgtccc gcgagcgttc
     1621 cggtgagggt cgagttctgg cccgaggcga ggagggcgaa ggcgaagacc gcgctcgctc
     1681 cggccacgcc gaggagcggc gtcaggagcc ggtaggcgtc ctggatctcg gccactccgg
     1741 agtgccctgt ggtgtggaac gtggcggccg cgacgatcag gatggccgcg ttgatgaaga
     1801 gcgcgaagct gagggcgatg gtggagtcga tgaacgcgta cttcaccgcc attcgcttgc
     1861 cctcgggcgt ctcctcgtac cgccgggtct gcacgatcga cgagtgcagg tacaggttgt
     1921 gaggcatcac ggtggcgccc aggatgccga tcgcgatgta gagtttgtgc tggtccgcca
     1981 ggatcgagcc gctcggaatg aatcccacgg cgatgccgga cagcgagggc ttcgcgatca
     2041 ggatctcgaa gaggaagcag gccgcgatcg tgccgatgag cgcgatgacg agcgcctcga
     2101 ccagcctgaa tcccttgttc tggaggtaga ggatcaggag cacgtcgagg gcggtgatcg
     2161 cgatgcccca gggaagagga atgtggaaga ggagattgag cgcgatcgcc gacccgatca
     2221 cctccgccag atcacaggcc gcgatggcca gctcgcagat gatccagagg aagatggaga
     2281 cggggcgcga gtagtggtcg cgacaggctt gcgcgaggtc gcgtccggtc acgatgccga
     2341 gcttgctcga gagtccctgc agcaagatcg ccatcaggtt cgagatcagg atcacgctca
     2401 gaagcgtgta gccgaacgcg gacccgcccg cgagatcggt ggcccagttg cccggatcca
     2461 tgtatcccac cgcgacgagg tagccggggc cggcgaacgc gagcgcgcgt cgccaccacg
     2521 ttccggtggg gaccggcacg gagcggtaga cctcggcgag gctcggcgtg acccgcggct
     2581 gtcgccagcc ggcatcggcg cgcggctcct cggacttctt ggccgacagg tgcacgattt
     2641 tctctctttc gttcaagtcc gccccgagcg gcggcggaag gtcgaagtga cgccgaggat
     2701 cccccgcgct acgttccatg ctaactgggc ggtaccgaag atgccgggcg ccttgaaccc
     2761 acgcccgcgc ttgtcgcgtc cgcgcgccac catccagatg gctccggcga cgctcgctag
     2821 ccacggcgcc ttttccccga tgtcccgtgc gattccgacc acgcggtcca ccgtgccgag
     2881 cggcgcgcgc cagacttcgg cactacgagc gatgtcctca cgatacgcgt ccgatcgcgc
     2941 gatcagctcg cgccggcggg cgaggagctc ttccttggac gccatttagg tgcgtccccg
     3001 cagcttgtcc cgatccttct tgaactcccc aatcgtggcc tcgaagaacc cggtgcgcga
     3061 gccgagccag cggcgcagat agagcacgcc cccgatcgag caggccagca cgaggaatgc
     3121 ggcgatgccc accgtcaacg cgcgggccga ctcgggcacg acgaggacga ggaagatcac
     3181 gccgaggagg aggccgagtt ggaggcaaag gaccacggac gccacgagga tcacgagctt
     3241 cgtgatgtcg gcgtgcgcga gtgcgagatc gtttccgagg agctcgaggc gcgtctcgat
     3301 cagcccgagc gtgctcgaaa tggtcctctt gagcgaggag atgatccccg gccggtcgtc
     3361 gcgttcgatg tcgtccatgg gctcggtcac cgtctcgcga gaaggattcc gatcacgatc
     3421 cccgcggcag ccgcgatccc caccgcattc cacgggtgct cgtgcacgta gtcgtcggcg
     3481 gtcttggcgg cttccttcgc gcgatcgagc gtggcgtcct cgagattgga gagcttcgcg
     3541 cgggcctttc cgatggtcgc ctcggcgcgg gcccgcgcgg cgctcacctt ttcgcctgcc
     3601 gcgtccttgg tcgcgcggag aagctcctcc gcgtcggaga cgacggcctt gagatccgcc
     3661 acgagctgat cgcgcgcggc gctgggagtg atgctgctcg attcggtcat ggttcttggc
     3721 tcctgacgcg gggcgcccgt gcgggcgccc caggttttag gccggctgcg gtctcgcgga
     3781 cgcgagcgcc ttgccgaact tgcgtgcctt gaacgcgagc atgacgagtg cgacgcccgc
     3841 gaggatggac cagatgccga tgatggtgag gagcgccagc gcgcccgccc ccggccgcac
     3901 gatcatgatc aggccgaaga ggaccgacgc gaagccgccc aggatcatcc accactcgcc
     3961 ctggatctcc cgccgaagcg cgaaggagag gacgatacgg atgatgccgc tgatgatcgc
     4021 ccacaccgcg atatagaaga gtagcgtgag cgcggccatt ccgggggagc ggagtgcgaa
     4081 gatgccgagc gcggccgcga gcaggccctc gaggagcatc atccaccacc tcggggtctc
     4141 cctccggccg ctaaaggcgt ggaagatcgt gaagacgccg tcgatcagga gccagacccc
     4201 gaaaaccagg atgagcgagg cgagggtgat gcccggcatg gtcagcgcga cgattccaaa
     4261 caggatcgcg gcgagtccgc ggagcagcgg gatccaccaa taccgctcga tcagggacga
     4321 cagcatgagg cccccctttc agcggtccgg gccgccgtgg ccggaccatc cgaggacggt
     4381 aaaccggcac cggggctatg gccaacgggg aaatgagtgg ctacactgcc gcccatgagc
     4441 gacctcgagg aatgcttcga agccaaccgg cagtgggcgg cgcgcatgcg cgagcgggag
     4501 cccgagtttt tcgagcggct gcggcatcag cagcagccgg agctcctctg gatcggctgc
     4561 tccgacagcc ggcttccacc ggaccgcatc atcggaaagc agccgggcga ggtgttcgtg
     4621 caccggaacg tcgcgaacgt cgtcgtgcac acggacatga actgcttgag cgtgctccag
     4681 tacgggattg aagtgctcgg tgtccgccac atcatcctct gcgggcacta cggctgcggc
     4741 ggcgtgctcg ccgccctcca gacgcagccc ttgggattga tcgacaactg gctccgccac
     4801 atccgggacg tccacgccgc gcatcgcgac gagctcgacg cgatcgcgga cgagggtgcg
     4861 cgcgcggacc ggctttgcga gctgaacgtc gcggcccagg tcgcgaacgt ctgccgcacc
     4921 accatcgtcc aggacgcctg gcgcgcgggg cgtcccctca cggttcacgg atggatctat
     4981 tccgtgagcg acggtttgct ccgggatttg gggctctccg tctccaagct ggaggatatg
     5041 cctagtcgct tccacatgag ctgaacccac accgaggagg ttcccatgcg tccattcctc
     5101 accgccgtct tcgtcctcgc ctcgatcctc gtcctgaccc agcctgccca agccacctgg
     5161 ggcgtcggcg ccaacatggg gctcacgatc cacaatcccg aaggtcccgg ggacgacgtg
     5221 accatgttcg ggattccgac ccaggcgaac gccctctcct cgatccggcc cggactccgc
     5281 gtgagctaca cgggcggggc gaagatgacc catgagggat tcctcgacct gagctacgac
     5341 cgccagtcgt ccgacgggga ccacttgagc accctccgtc tcgccggcaa ctaccagtac
     5401 aacttcacga tgtccggctc tccgcgtccc tacgtcacgt tcggggtcgg catgttccac
     5461 gagggcttcg atactggggg cggggatgtc ggcgccaccg ccatgacgtt cggcggcggg
     5521 ttcggcgtgg gcttcccggt cagcaagggg gcagggcgga tccgcaccga ggtgcgcatg
     5581 gacaagctcg acgacgcgga cgacgacgga ttcccgatca tcggcgaagc gacggtgatt
     5641 cagatcggcg cggggttcga cctctggttc aagtagctac agcgtcctct tgaacatcgt
     5701 cgatgccagc acgagcatga tcccggcgaa cgcggccatc accgcgaaat ccggcgccac
     5761 cgcgctgaag ccgacgcctt tgagcagcag attccggagc gcatgcaccg cgtaccgcat
     5821 ggggaacgcc atcgagatcg cgttgagcca gtggggatag ctctcggtgg ggtagagggc
     5881 gccgctcggg aagaacgtca ccacgttcag gattccgaag agggcgcgcg gggtcatcgg
     5941 gttcgacacg cgggccatca acgtgaacat gaacaggatc aacccgagcc cgaggaggaa
     6001 gaccgccgcc tccgtcatga ggatcgtggg caagccgccg atgatcggga gccgggcgat
     6061 caggatcgag gcgaccagca cgatcgttcc gatgatcatg ctgagcgaaa cggccgagat
     6121 cgtgagcccg aggaccaggt ccagcgagga tagcggcgtg acgaagtagc cttcgtggat
     6181 gcccctcgcg cggtcgtcca cgaggatgat gccccccgcg agcatggcca cgaagaagag
     6241 cgcgagcgcg atcacgccgg gcacgaggta ctgcatgaac tccttgtgcc cgtacacgtc
     6301 cacccgctcc acctgcactc cagggaacac gggcgccgac gtcacggcgc ggagctccgt
     6361 gttcacgccc aggaaggcgc cccgtagctc ctgcacgatc acgttcgccg cggtgttgtc
     6421 cgtgttgtcc acgaccaccc caagcggcgc gggctccccg cgcgcgtagc gatcgctgaa
     6481 gccggggggc acgatgacca cgcccttgta gcgcccctgc cggagctccc gcaccgcgga
     6541 gctcgggtcc ggctcgctcg agagctggaa gagctcgcgg ccctgggcca tggtcacgag
     6601 ccggttcagg acttccgccg acgccgcgcc gtgatcctgc accacgaccg cgaccggaag
     6661 gttgtggaga tccccgccca tcgacttccc gaggatgacg agatagatga tgggcatgag
     6721 cgccatcgga accatgagct tgctgttacg ccggaagcgg cggaggtcgc gctcggcgac
     6781 ggcgaggcag cggctcaggc tgaagtgcat catcagcgca tcatcgaggg gaccttgtaa
     6841 ccctggtact cctcgctcgc cg
//
LOCUS       DASTKC010000220        17227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2081777, whole genome shotgun sequence.
ACCESSION   DASTKC010000220 DASTKC010000000
VERSION     DASTKC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17227
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081777"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFR25_09150"
     CDS             <1..240
                     /locus_tag="VFR25_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941261.1"
                     /translation="GYSIGEVSCPTKYFPEASSISFARSVRYGLGVLWCCFQFRLQRW
                     GLRSYRIFDPSGRKIEIDPVPSHSAPVGAVSESPA"
     gene            237..1709
                     /locus_tag="VFR25_09155"
     CDS             237..1709
                     /locus_tag="VFR25_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941262.1"
                     /translation="MTERRGPALLLLAFLLGIGIFYTLTLRPGHDWGDDFAQYIHHAK
                     NIATGVSYSQTGYIWNPRFPMLGPPTYPPVFPLMLAPVVAASGLALTPMKGVVVASFL
                     LALLFVALVFRSILAPRYLAVLLVLLGLNPYFWNLKDNILSDLPFFLFVYIGLYLIQR
                     RPEPAEGSGRVALHGIALGVAFYLAYGTRSLGIVLVPCAIAAEWLSRRRVGRGTGIAV
                     LVFAALAGLQAALAHKDSGYAGIVSVDPRRIAINLVEYARYLSGLWNNAWWGAADTLV
                     LILVLVLAAMGFLARLRARPGTLEIFTVLYGLAILPWIATQGRYLIPLMPLYVGYALW
                     AVQVAAKHSPARGRLILAGLLVLALAGFCSVYLSTPLRRPSEGVGTPDAIALWGVVDR
                     ITRPGDVVIFQKPRALALFANRSASGVSNTANDEETWQYFESVRARYAILGPDDHVFL
                     NQDRIRRLVEARPERFERVYENPEFTMYRIRPDGEGAPGR"
     gene            1706..2449
                     /locus_tag="VFR25_09160"
     CDS             1706..2449
                     /locus_tag="VFR25_09160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9941263.1"
                     /translation="MTKDARRPTARERCSKLRERYYDDAGYPGPLDRYREAIARHAGG
                     AVAVLDAGCGATMPFLREIVPLTRHAVGVDLERLRRDGAGPDGCRADVGRLPFKDESF
                     DLIISMSVLEHLDDPPAVFRELGRVLRPGGKLVVQTPNRFDYVSLIAHATPFSFHRWI
                     VPRVMNREEEDVFPTRYRANTRGDLTRAMKAGGLEPRSIESFNQYPAYLMFSPLAFRL
                     GMLYERLTTRVAALAWLRGWILAVGEKPA"
     gene            complement(2446..3762)
                     /locus_tag="VFR25_09165"
     CDS             complement(2446..3762)
                     /locus_tag="VFR25_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941264.1"
                     /translation="MTRRSTPTLLALLVLTIAMVVEARPTPAAVNPALTNIPNNTWVQ
                     LNSKSYDANGTDITVAGGWPCNSYSALVYDPDHRALLMFGGGGHGGRRGNDVWMYDIG
                     SDAWRMQYFPDPETSYPYSVDDSGMTFAQYCNSTDPMTCNPSAAWLPRGTTTNKRPWT
                     SHSFDQLSYDETNHKLVLFGPNFIFGYGSEHYYGVPDAYAYDVASKTWSHYSSIPKLY
                     HQMSRSAYDPVHKLVVAMGRTWTAPGNSWLATAECWTLNVTTGVWTKKATPPIWGSDA
                     NLVWDSVNNVMLMYGADYPTTADLWAYDPGTDTYQQKTTLPDPTYGMPPGGAPNAAFD
                     SAHGILLILGRSDAPYIPTWAYDVRTTRWKKMNATNEPGTDKVIIGSQLAYDPENNVF
                     FLNALRGDFSSTGGFYGERGELYAYRYGPSAPDVTPPSAVRNLTSP"
     gene            complement(3801..5375)
                     /locus_tag="VFR25_09170"
     CDS             complement(3801..5375)
                     /locus_tag="VFR25_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941265.1"
                     /translation="MKRWTVVAAGWALLMLTVGAGASRAQTCSPTQTSQSQILPDVQP
                     APKPALFATFTDPLYGTCFKRISDDQMYPSSTHNPVPIYPQLQAWNADQTLLILATGD
                     LLRSPSYAYYKRLPLGGANFRWSPIQPNIGYFSQSNRFVKMDVNTDAITTLHTFNEYP
                     GGLERGNEWEDVSRDDRFVVLEGYRTAAQKDTVQVSGISMTTGSRTISSAGRFGQVLV
                     RMPVYGTGIPAGTTVQTVTNSSSLTLSQSCTITSGNATLAFGASEVFVYDFKNDRKGT
                     VRPGFSGIGLCGGIDDMLMSPSGKYALVHWGTGGNGQTCHMQAYDTSMAYVGQVLCGR
                     GHYDLTVDKDGSEWAVTFTTNAGCGETGAYLAKYRIPDGATRLAAGDSSGFSRLCVFS
                     DLIGGGHVSGRAYGAGFVVLSADHPTAGSPPAPFFKELVKVYLDSRPEAPHLERLVNH
                     RADEAQIYADGACPLSSYWAQSHATISRDGTKVLWGSNWNTAGANCAAEAYVMDLAPA
                     APDNIPPGAIRDLQGR"
     gene            complement(5398..6717)
                     /locus_tag="VFR25_09175"
     CDS             complement(5398..6717)
                     /locus_tag="VFR25_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9941266.1"
                     /translation="MILAGSSLAGVSIRADAAQKDLYTGSSFETAVESLAPGDTLTVH
                     AGTYDGTGRISITVPGTAARPVLVRGAPDEARPLITRPAGSTAQNTINIEGASYLTMS
                     NLEISGNGDGINMSLGPHHITIEDCEVHHVDVGINFRSSMNHVTVRRNHVHDTGMDGS
                     TGEGLYVGCNDGSCAVSESLIENNWIHDTMAATQGDGIEIKLGSWGNVVRDNVVYNTK
                     YPGILLYGTMGNARNVVERNALWNCGDSGIQAAADAVIRNNLILESPGNGFNSQDHNG
                     VTPNRIEFVNNTIVGGSPVLRLSNWANKQGMILANNAIYSSGGYSVGSLTGVVVTGNV
                     AYPATSALPATGVTTGYGTSVDFVNATARNVYPSVNSKLLDVANAVYAPSDDFNGTAR
                     TGVPEAGAYTWTQQANPGWVIGPGFKYPASLVADGIPPAAIRDLYGR"
     gene            7313..8281
                     /locus_tag="VFR25_09180"
     CDS             7313..8281
                     /locus_tag="VFR25_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941267.1"
                     /translation="MAALQLAVTPLCSVAQTGAANSKKLDWTAPGDDGSLGRASRYEL
                     HYSTSAISDAADSTALDAWWSSQSSPVPGMPLPSQSGAIDSVRVNNLTYGTTYYFIIR
                     TVDKAQNWSPYSNVAMVAVAPCDAPTSTPGTPQVQTDSMGVLLSWDPPQDPLATSVQV
                     YRGPSAGKLALFGSVPVGEASYHDTTVRSGTTYVYALAWSASCGNGPLGSTASVTIAN
                     PNPPAAPHVVASGGSLRVYPNPAPGPLTVRVRIDGAQPQAVRVRLYDMSGHWIADLAD
                     GTYSAGETLLTWNRQTRGGSPAAPGYYEALGTIGTTRVREQLVLLP"
     regulatory      8468..8575
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8635..9516
                     /locus_tag="VFR25_09185"
     CDS             8635..9516
                     /locus_tag="VFR25_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941268.1"
                     /translation="MRRPATHRRHSIRRTTNVLPSLRSGGLACLAFLALLVFAAPPAR
                     GQVNPDSSVVLQWTAPGDDGTSGRASVYDIRYRTSAISGTDTTTWWNGATQVSGEPFP
                     GNPGANDSMRVRGLAPVTTYYFLIKTGDEVPNWSGFSNLAQKSTSGDVVAPAAISTLA
                     VTGVGTNSLSLRWTSTGDDNTTGTAASYDVRYSTSTITAGNFASATAATGEPAPLVSG
                     TVQNFNLTGLSTGQIYYVAIKVTDERGNASLISNVVSGTPADTIAPAAVRDLSLGPQS
                     PMSFALADLEEHRAFDF"
     gene            9500..10213
                     /locus_tag="VFR25_09190"
     CDS             9500..10213
                     /locus_tag="VFR25_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941269.1"
                     /translation="MPSISSPRRYAIASALLGALLLVALAVPETARAADVVLHWTAPG
                     DDGNVGTASMYMIRYSLQPLAGSQPADSTDWWTNLSTPVGGSIPTPRVAGTREMFTMT
                     GLDSGTVYYYMIRTCDEIPNCSGYSNFAMKVGGVNPTTVESADMIGYPNPASDHVTFR
                     FQAGTKSGSPGPARLTIYDLNGRRVIELLNEVLPPGEQTVVWACQSDAGNHVAPGLYN
                     AILEAPTGREIRQIVVLPE"
     gene            10301..11161
                     /locus_tag="VFR25_09195"
     CDS             10301..11161
                     /locus_tag="VFR25_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HET9941270.1"
                     /translation="MSVDVEDYFQVQAFAHHAPRDGWDRWPSRVEGNTQRLLDLFDEA
                     GATATFFVLGWVARRHPSLVRQIASRGHEVASHGMEHRMLTEQSPESFAADARDSKVL
                     LEDVSGAPVLGFRAPSYSIGRSTLWAVDVLADAGYAYDSSVYPIRRQRYGYPEGPDRP
                     AMIHGPRRALPEFPLPTLGLGSWRVPVLAGAYLRLWPSWISRFAAGEHLRRRRPLVVN
                     VHPWELDPDQPTIGGARWRTWTHYTGLGRTQSVLRAVLSRARFRDIATRLRELGLLDG
                     RIARAGEAAS"
     gene            11158..12615
                     /locus_tag="VFR25_09200"
     CDS             11158..12615
                     /locus_tag="VFR25_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941271.1"
                     /translation="MNLDVLILVAGGVIAILSAALVLALSPARYPSLVAAGSLAALGV
                     LQFGWARAASPFMGGENRIWFELSLAFAVVVSLLWLLLSRTIGLGPRPSPIGPWRWYI
                     LAQSLCAAAAVARLALEPTYEEMQQIGGGFAYGLGPFTTLLIVAQLLNLILLTASFES
                     TYFALPASPRRAFLPGLIGILAWTGYFTYVWIQSLILGSVHVGDLSTGAFAVMDIAVL
                     LPISLIRGRLGEAHVRREPRPLARTMSVGFTLAFLLATTGLLWIAHATGWSLARSVWI
                     VLGLLGALGLAALMISNRVQRRVQRWIDPYLYGGVLDRTGIERRADEMLAVSRSAADV
                     HGVIPSTTEEIVGARPVTLFLASSDGTRFIVAASTIAPAPPVSVDVEAPLAIELRRAG
                     RAIRLRGRPDDLEYIPIYVENAGQLEACDAVCAAPLLVDEEMAGFLLCGERGSDPTSR
                     RQLLPLLDLLCARYSEALTRVSFRAASSEPRAVDV"
     gene            12856..13200
                     /locus_tag="VFR25_09205"
     CDS             12856..13200
                     /locus_tag="VFR25_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor N protein"
                     /protein_id="HET9941272.1"
                     /translation="MGEVQPVICRSRNIIFGLIAATLFAANTAHAIPALQLYSPDAIY
                     DVSSASWIINKSTFELYVIGDVEKVGTIYDVDLVASVYGSGGNITITPNLAIDSTPED
                     AAAYSNVINHAE"
     gene            13231..13446
                     /locus_tag="VFR25_09210"
                     /pseudo
     CDS             13231..13446
                     /locus_tag="VFR25_09210"
                     /inference="COORDINATES: protein motif:HMM:NF038141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor N protein"
     gene            13429..13557
                     /locus_tag="VFR25_09215"
     CDS             13429..13557
                     /locus_tag="VFR25_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET9941273.1"
                     /translation="MFAPFSHDATEGNPPGAPEPGSMLLVGLGLGVAALTGRRKKS"
     gene            13665..14792
                     /locus_tag="VFR25_09220"
     CDS             13665..14792
                     /locus_tag="VFR25_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941274.1"
                     /translation="MTAKRVRGPVVLALLLLIAAPAMAQSPPGSQPTTSPQNPPTNPD
                     AVKPPPPPILTLFTVPRDADVVLKGRTDLAGRTPLDFPVTVEGKYSVRLRGTGYPPVH
                     GVIYIPPRGQLAFVLSEPREGSLGLFMRGLNYPGVPQLGTGRTSRGVTLVTYASGGIF
                     MAAKAHSDYRERLDEVGEFPEDRARDERRYRNGWLIFTGATWAMSALDYWTRPRFDLA
                     ETTPSRLTLDVPTITRVGAVWRSLVVPGGGQDFANHRTRGTVWLATVIAAGAGYVVTD
                     YRVNRDETDVKWAKIAVNDAGPGTIDQKLLELEQQERDLSASQDARRGFAIAGIALYS
                     LNLLDSMIMPLGYNPPKRAKVASIEPIVDPRMAGMSVNVRF"
     gene            15220..16653
                     /locus_tag="VFR25_09225"
     CDS             15220..16653
                     /locus_tag="VFR25_09225"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET9941275.1"
                     /translation="MYADVRSSYAAVAVGGSTGDFLQFEVGGRASGMGGAQVSSASGV
                     MAQFWNPAGLASLERPQVGGMHATWLDNLSYEWLGYATPVNPRFGVGSVSLAYFHMPS
                     IQGADEFGNPTGDFRVYDMALTAGLAKPVGRHLSVGGNAKMIRQNLATVSATGFAVDL
                     GASYMTHGATLAAVVQNLGPELSYGTGGSYPLSRQIRFGLSREWLSGKLLLGADYNMP
                     SDYYDDARFGAEYRLHPNVSMRMGYRHQFGELDDPAEGISYGLGVHFGQLNVDYALTP
                     DNDFSDVHRLSFGYSFGSSAKEAEPKQPKHEEPKPEPPAPTGPKVIAQAPAPKPAPAP
                     KPTPAQTKPAPQADAPKTEIVALAPAAKPEAEAPKAEAVAPPPAPAVATQAPSKEPEA
                     KPAPAGEVVYLVVLPGYPSKESAQAEMKALELLGFRTKDAQIARDPKKGGYRIAFVKL
                     KNKGNAEEVASDLQRMSFRALVEVAQK"
     gene            16905..>17227
                     /locus_tag="VFR25_09230"
     CDS             16905..>17227
                     /locus_tag="VFR25_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9941276.1"
                     /translation="MLLPAELKNRDQDGLSTADVLYSWFKRSLDILLSSVGLVVMAPV
                     FAVIAAVIKVDSRGPVLYGQERIGINRRRSDGTRRSTDERRKRDTFGRPFKIWKFRTM
                     IVDAE"
BASE COUNT         2719 a         5909 c         5527 g         3072 t
ORIGIN      
        1 ggctactcga tcggcgaggt gagctgtccg acgaaatact tcccggaggc atccagcatc
       61 tcgttcgcac ggtccgtccg atacggactc ggcgtcctct ggtgctgctt ccagttccgg
      121 ctgcagaggt ggggccttcg ctcctaccgc atcttcgatc cgagcgggcg gaagatcgag
      181 atcgatcccg tcccgtccca ttccgccccc gtgggggccg tctccgagtc cccagcgtga
      241 cggaacgccg cggtccggcg ctcctcctcc tcgcgttcct gctgggcatc ggaatcttct
      301 acacattgac gctccggccg ggccacgact ggggcgacga tttcgcccag tacatccatc
      361 acgcgaagaa catcgcgacc ggcgtttcct acagccaaac cggatacatc tggaaccccc
      421 gcttcccgat gctcggtcca cccacctatc cgccggtctt cccgctcatg ctcgcgccgg
      481 ttgtcgcggc gagcgggctt gccttgacgc cgatgaaggg ggtggtcgtc gcctcgttcc
      541 tgctggcgct cctcttcgtc gcgctcgtct ttcgcagcat cctcgcgccg cgctacctgg
      601 cggtgctcct cgtgctgctc gggctgaatc cctacttctg gaatctcaag gacaacatcc
      661 tgtccgacct gccgttcttt ctctttgtct acatcgggct ctacctgatc cagcgacggc
      721 ccgagcccgc ggagggatcg gggcgtgtcg ctctccacgg gatcgcgctc ggtgtcgcgt
      781 tctatctggc ctatggaaca cgcagcctgg ggatcgtgct cgttccatgc gccatcgccg
      841 ccgagtggct gagtcgccgg cgcgtgggca gggggaccgg gatcgcggtt ctcgtctttg
      901 cggcgcttgc ggggcttcag gcagccctgg cgcacaagga cagcggctat gccggcatcg
      961 tgagcgtcga tccgaggcgg atcgcgatca acctcgtgga gtacgcccgc tatctgtccg
     1021 ggctctggaa caacgcctgg tggggcgcgg cggacacgct cgtcctgatc ctcgttttgg
     1081 tgctggcggc gatgggtttc ctggcgcggc tccgagccag gcccggaacg ctcgagatct
     1141 tcaccgttct ctacggcctc gcgatcctcc cctggatcgc gacccagggg cggtatctca
     1201 tcccgctgat gccgctctac gtgggttacg cgctctgggc ggtccaggtc gcggcgaagc
     1261 actcgcccgc gcgcggccgg ttgatcctcg cgggcctcct cgtcctggct ctcgcggggt
     1321 tctgtagcgt ctacctgtcg actccgctcc ggagaccgtc ggaaggggtc ggcacgcccg
     1381 acgcgatcgc gctctggggc gtcgtcgacc ggatcacgag gcccggcgat gtcgtgatct
     1441 tccagaaacc gcgcgccctc gccctgttcg cgaaccggag cgcctccggc gtcagcaaca
     1501 ccgcgaacga cgaagagacc tggcaatact tcgagagcgt gcgcgcgcgc tatgccattc
     1561 tgggtcccga cgaccacgtc ttcttgaatc aggaccgcat ccgtcggctc gtggaagcgc
     1621 ggccggaacg gttcgagcgc gtgtacgaga atccggagtt cacgatgtac cgaattcgtc
     1681 cggacgggga aggagcacca gggcggtgac gaaggacgcg cgacgcccga cggccaggga
     1741 gcgctgctcc aagcttcgcg agcgctacta cgacgacgcg gggtatccgg gccctctgga
     1801 ccgctaccgg gaagccatcg cccgccatgc cggaggggcc gtcgcggtgc tcgatgccgg
     1861 ttgcggagcc acgatgcctt tcctgcgcga gatcgtgccg ctcacgcggc acgcggtggg
     1921 agtcgacctg gagcggctac gtcgagacgg cgcggggccc gacggatgcc gcgcggatgt
     1981 cggacgtctc ccgttcaagg acgagagctt cgatctcatc atctcgatga gcgtactcga
     2041 gcacctggat gaccccccgg cggtcttccg agagctcggc cgcgtgctca ggccgggagg
     2101 gaagctcgtc gtccagactc ccaaccgctt cgactacgtg tcgctgatcg cgcacgccac
     2161 gccgttctcg ttccaccgct ggatcgttcc gcgcgtcatg aatcgggaag aggaggacgt
     2221 ctttcccacg cgctatcggg ccaacacccg gggagacctg acgagggcga tgaaggcggg
     2281 agggctcgag ccccggagca tcgagagctt caaccagtac ccggcctacc tgatgttctc
     2341 gccgctcgcg ttccggctcg gaatgctcta cgagcggttg accacccggg tcgccgcgct
     2401 ggcctggctt cggggatgga tcctcgccgt gggggagaag cccgcctagg gactcgtgag
     2461 gtttcggacg gcgctgggag gcgtgacatc cggcgcgctg ggaccgtacc ggtaggcgta
     2521 gagctccccg cgctcgccgt agaaccctcc cgtcgagctg aagtcacctc gcagcgcatt
     2581 caggaagaac acgttgttct cggggtcata ggcgagctgg gatccgataa tgaccttgtc
     2641 cgtcccgggc tcgttcgtcg cgttcatctt cttccatcgc gtcgtgcgga cgtcgtacgc
     2701 ccaggtgggg atgtacggcg cgtcgctcct cccgaggatg agcaggattc cgtgcgcgct
     2761 gtcgaacgcc gcgttgggag cccctcccgg aggcatcccg taggtcgggt cgggtagggt
     2821 cgtcttctgc tggtaggtgt cggtccccgg gtcgtaggcc cacagatccg ccgtcgtcgg
     2881 gtagtccgcg ccgtacatga gcatgacgtt gttcaccgag tcccagacga gattcgcgtc
     2941 gctgccccag atggggggcg tcgccttctt ggtccagacc ccggtcgtca cgttgagcgt
     3001 ccagcattcc gcggtggcga gccacgagtt gccgggcgcg gtccaggtgc gtcccatcgc
     3061 cacgaccagc ttgtgaacgg gatcgtaggc cgagcggctc atctgatgat agagcttcgg
     3121 gatcgagctg taatgcgacc acgtcttgct cgcgacgtca taggcgtatg cgtcgggaac
     3181 gccgtagtag tgctccgatc cgtagccgaa gatgaagttc ggaccgaaga gcaccagctt
     3241 gtgattcgtc tcgtcgtacg agagctggtc gaagctgtgg gaggtccagg gacgcttgtt
     3301 cgtcgtcgtt ccgcgcggga gccaggccgc gctcggattg caggtcatgg ggtcggtcga
     3361 attacagtac tgggcgaagg tcattccgct gtcatcgacc gaatagggat acgacgtctc
     3421 cggatcgggg aagtactgca tgcgccacgc gtccgagccg atgtcgtaca tccatacgtc
     3481 gttcccgcgg cggccgccgt gcccgccgcc tccgaacatg aggagggcgc ggtggtccgg
     3541 gtcgtagacg agcgccgaat aggaattgca cggccagccc ccggcgaccg tgatgtccgt
     3601 tccgttggcg tcgtacgact tggagttcag ctgcacccac gtgttgttgg gaatgttggt
     3661 cagcgccgga ttgacggcgg ccggcgtcgg gcgcgcctct acgaccatgg cgatcgtcag
     3721 cacgaggagc gcaagcagtg tgggcgtcga tcgacgggtc acgtagtgct cctttcgaga
     3781 gccacgaaac gacggggcgc tcatcgtccc tgcagatccc ggatcgctcc ggggggaatg
     3841 ttgtccggcg ccgccggcgc gagatccatg acatacgctt ccgccgcgca gttcgctccg
     3901 gccgtgttcc agttgctccc ccacaagact ttcgtcccgt cccggctgat ggtcgcgtgc
     3961 gactgggccc agtacgagga gagagggcag gctccatcgg cgtagatctg cgcctcatcc
     4021 gctcggtgat tgacgaggcg ttccaaatgc ggggcctcgg gacggctgtc caggtagacc
     4081 ttcaccagct ccttgaagaa cggagccgga ggtgagcccg cggtgggatg gtccgcgctc
     4141 aggacgacga agccggcccc gtaggcgcgg cccgagacat gaccgcctcc gatgaggtcg
     4201 ctgaacacgc agagtctcga aaaccccgag ctgtcgcccg ctgcgagccg ggtggcgccg
     4261 tccgggatcc ggtacttggc cagatacgcc ccggtctcgc cacagcccgc gttcgtcgtg
     4321 aacgtgacgg cccactcact cccatccttg tcgaccgtca ggtcgtagtg gccgcggccg
     4381 cacagcacct gcccgacgta ggccatcgac gtgtcgtacg cctgcatgtg gcacgtctgc
     4441 ccgtttccgc ccgttcccca gtggacgagc gcgtacttcc cggacgggga catgagcatg
     4501 tcgtcgatgc caccgcagag cccgatcccc gaaaagcccg gccgcacggt gcctttccgg
     4561 tcgttcttga agtcgtatac aaagacttcg ctcgcgccga aggcgagggt cgcattcccc
     4621 gaggtgatcg tgcacgactg ggagagggtc agtgacgagg agttcgtcac cgtctgcact
     4681 gtggtccccg cggggatgcc ggtgccgtac accggcatgc ggaccaggac ctggccgaac
     4741 ctgcccgcgg acgagatcgt ccgcgatccc gtggtcatcg agatcccaga cacctggacg
     4801 gtgtccttct gggccgccgt gcgataaccc tcgagcacga cgaatcggtc gtcgcgggac
     4861 acgtcctccc actcgttgcc gcgctcgagg ccgcccgggt actcgttgaa cgtgtggagc
     4921 gtcgtgatgg cgtcggtgtt cacgtccatc ttcacgaacc ggttcgattg cgagaagtag
     4981 ccgatgttcg gctggatggg ggaccaccgg aagttcgctc cgccgagggg aagtctcttg
     5041 tagtacgcgt agctcggact acggagcagg tcgcccgtcg cgagaatgag aagcgtctgg
     5101 tccgcgttcc aagcctggag ctggggatag atgggcacgg gattgtgggt cgaagacgga
     5161 tacatctggt cgtccgagat tcgcttgaag caggtgccgt agagcggatc cgtgaacgtc
     5221 gcgaagagcg ccggcttcgg cgccggctgg acatcgggca agatctggct ctggctggtc
     5281 tgggtcgggc tgcaggtctg ggcccgggat gcgccggccc ccacggtcag catgaggaga
     5341 gcccagccgg ccgccacgac ggtccatcgc ttcatcgaag cacccttcct cacgcgatca
     5401 ccgtccatac aggtcccgaa tcgcggccgg cggaatgccg tcggccacca agctcgccgg
     5461 atacttgaac cccggcccga tcacccatcc cgggttggcc tgctgcgtcc acgtgtaggc
     5521 gccggcttcg ggaacgccgg tccgcgcggt cccgttgaag tcgtcggacg gcgcatacac
     5581 tgcgttcgcg acgtcgagga gcttcgagtt cacgctcgga tagacgttcc gcgccgtggc
     5641 gttcacgaag tccaccgagg tgccgtagcc cgtggtgacg ccggtcgcgg gtagggcgga
     5701 cgtggcggga tacgcgacgt tgcccgtgac gaccacgccg gtcaggctgc ccacgctgta
     5761 gccgccgctc gagtagatcg cgttgttcgc gaggatcatg ccctgcttgt tcgcccagtt
     5821 ggaaaggcgg agcacaggcg agcctccgac gatggtgttg ttcacgaact cgatccggtt
     5881 cggggtcacg ccgttgtgat cctgcgagtt gaacccgttt ccgggggact cgaggatgag
     5941 gttgttccgg atgaccgcgt ctgcggccgc ctgaatgccc gaatcaccgc agttccatag
     6001 agcgtttcgc tcgacgacgt tccgcgcatt ccccatcgtt ccgtagagga ggatgccggg
     6061 atacttcgtg ttgtacacaa cgttgtcccg gacgacgttc ccccaggacc ccagcttgat
     6121 ctcgatgccg tccccctggg tcgcggccat cgtgtcgtga atccagttgt tctcgatcag
     6181 agactcgctc accgcgcagg agccgtcgtt gcacccgaca tagaggccct cgcccgtgct
     6241 gccgtccatg cccgtgtcgt ggacatggtt ccggcggacg gtcacgtggt tcatcgagct
     6301 ccggaagttg atgcccacgt ccacgtgatg gacctcgcag tcctcgatcg tgatgtggtg
     6361 cgggccgagg ctcatgttga tgccgtctcc gttgcccgag atctcgaggt tcgacatcgt
     6421 gagataggac gcgccctcga tgttgatcgt gttctgcgcg gtcgaaccgg ccggcctcgt
     6481 gatgagcggt ctggcttcgt cgggcgcgcc gcgaaccaga accggccgcg ccgccgtccc
     6541 cggaaccgtg atgctgatcc ggccggttcc gtcgtacgtg ccggcgtgca ccgtcagcgt
     6601 gtccccaggc gcgagcgatt ccaccgcggt ctcaaagctc gagccagtgt agaggtcctt
     6661 ctgggccgcg tcggcgcgaa tcgagacgcc ggccaggctg gagccggcca gtatcatccc
     6721 agaaatgagg cacccggcgc tcgcgcggcg gggtagcatc atgtcacgaa ccctttctgt
     6781 ggtaacggga agactagaga aatgtccgca agctccatgc caccatgcgg aacgccccgc
     6841 agcaagggta actcattcgg tcgccgtgcg gttcccaccg gggatcgatc cattccgtcc
     6901 acagtctgta cggcaatgca acgcagagcg gtgcgcctcg ggtggctcgc ggagagaggc
     6961 gcggcatctt tgatctcctg gcttcgtccg acctccatgt cgactcgtcg acaccgaagc
     7021 gatgtccaac atgcttctcg agtgtgttcc catggatccg gagctccgtc gccggatggt
     7081 cgcgggcggg aacgcatcga gcagcgccgc ccgggtggga cggatggtgc gcgactacga
     7141 cgccctgtta gcatatccag catcgcccgg gtcggacggg gggtacccgc ggatggatcg
     7201 acccttgcat agtgcacagc cagcgctctc cagactcgca aattcgatag atgccataca
     7261 cggaggtagg gtgactgcac cccacggtgc acgtgtgtcc gcggcggtac tcttggccgc
     7321 gcttcaactt gcggtaacgc ccctctgctc ggtcgcgcag acaggcgccg ccaattcgaa
     7381 gaagctcgac tggaccgccc ctggagacga cggtagcctg ggccgagctt cgcgctacga
     7441 gcttcactac tccacgtccg ccatcagcga cgccgcggac tcgaccgcgc tcgacgcgtg
     7501 gtggtcgagc cagtcgagcc cggtgcccgg aatgccgttg ccgtcccagt ccggcgcgat
     7561 cgattcagtc cgggtgaaca atctcaccta cggtacgacc tactacttca tcatccgcac
     7621 cgtggacaag gcgcagaact ggtcgccgta ctccaacgtc gcgatggtgg cggtggcgcc
     7681 gtgcgacgct cccacgtcca ctcctggaac gccccaggtc cagacggact ccatgggcgt
     7741 tctcctttcc tgggatccgc cgcaggatcc gctcgcgaca tcggtccagg tctaccgcgg
     7801 cccgtccgcc ggcaaactcg ccttgttcgg atccgttccg gttggggagg cgagctatca
     7861 tgacaccacc gtgcggagcg gcacgaccta cgtctatgcg ctcgcctggt ccgcgtcgtg
     7921 cggcaacgga ccgctgggaa gcaccgcctc ggtcacgatc gcgaatccga acccgcccgc
     7981 cgctccacac gtcgtcgctt ccggcggaag tctgcgcgtc tatcccaacc ccgcaccggg
     8041 tccgctcacc gtccgcgtcc ggatcgacgg cgcgcagccg caggcggtgc gcgtccgcct
     8101 ctacgacatg agtgggcact ggatcgcgga tctcgccgac ggcacgtact ccgcgggaga
     8161 gaccctgctc acctggaatc gccagacgcg tggaggcagt cccgccgctc cgggatatta
     8221 cgaagcgctc ggcaccatcg gcacgacccg cgtgagggaa cagctcgtcc tcttgccctg
     8281 atccccgtgc gatctcgatg aggcgcggat gcaattcacg tctgcgactc tcgtgcaatg
     8341 tgcaggcttc atccccgccg tgacctaact cgctcattac caccgtccga tcccgtgcgg
     8401 aacgcacgcc aggcgtggca ccgttcgtgc tgctacgcag ttccgggggt cgaaacttca
     8461 ccacgtccat gactcagagc aaactgtccg aaagggcagg acgcagagct gcggggcttc
     8521 cgatcgtgag cggcggagta cgaccgcacc cgatcctgcc ggccgggccg ccatggatcc
     8581 ttgaacgccc gcgcgcctcc tcgaggcgcg ttggacgcac cggggaggac atccatgagg
     8641 cgaccggcca cgcatcgcag acacagcatc cgccgcacga ccaacgttct cccatcgctc
     8701 cgctccggag gccttgcctg cctcgcgttc ctggccctcc tcgtcttcgc ggctccgccc
     8761 gcccgcggcc aggtgaatcc cgattcgtct gttgtgcttc agtggaccgc ccctggtgac
     8821 gatggtacga gcgggcgcgc gagcgtctac gacatccgct accggacgtc ggccatctcc
     8881 ggcaccgaca cgaccacgtg gtggaacggg gcgacgcagg tgagcgggga gccgtttccg
     8941 ggcaacccgg gggcaaacga ctccatgcgg gtgcgcggtc tcgcgccggt cacgacgtac
     9001 tacttcttga tcaagacggg agacgaagtg ccgaactggt ccggcttctc gaacctggcg
     9061 cagaagtcga cgtcgggcga cgtcgtggcg ccggccgcga tcagcacgct cgccgtcacg
     9121 ggcgtcggga ccaactccct ctcgctccgc tggacgagca ccggggacga caacaccacc
     9181 ggcaccgccg cgagctacga cgtccgctac tccacgtcga cgatcacggc tgggaacttc
     9241 gcgtccgcga ccgccgccac cggcgagccg gcacctctgg tatctggaac ggtgcagaac
     9301 ttcaacctga ccggcctgtc cacggggcag atctactacg tcgccatcaa ggtgaccgac
     9361 gagcggggga acgcctcctt gatctcgaac gtggtgagcg gcacgccggc cgacacgatc
     9421 gcgcccgcgg ccgtccggga tctctcgctg ggtccgcaga gtccgatgag ctttgctttg
     9481 gccgacctgg aggagcatcg tgccttcgat ttctagtcct cgtcgctacg ccatcgcgag
     9541 cgcgctcctc ggtgcgctcc ttctcgtcgc gctcgccgtc cctgagacgg cgcgcgcggc
     9601 cgacgtcgtg ctccattgga ccgctccggg agacgacggc aacgtcggaa ccgcgagcat
     9661 gtacatgatc cgctactcgc tccagccgct cgccggatcc cagccggcgg actcgaccga
     9721 ttggtggacg aacctctcca cgccggtggg gggctccatt cccacgccgc gcgtcgcggg
     9781 gacgcgcgag atgttcacga tgacaggcct cgattcgggg accgtgtact actacatgat
     9841 ccgcacctgc gacgagattc cgaactgctc gggctattcc aacttcgcca tgaaggtcgg
     9901 cggcgtgaat ccgacgacgg tcgaatccgc ggacatgatc ggctacccga atcccgcatc
     9961 ggaccacgtg acgttccgct tccaggccgg aacgaagagc ggatcgccgg gaccggcccg
    10021 gctcacgatc tacgacttga acggccgccg cgtgatcgag ctcctgaacg aggtcctccc
    10081 cccgggcgag cagaccgtgg tctgggcgtg ccagtcggac gcggggaacc atgtcgcgcc
    10141 ggggctctac aacgcgattc tcgaagcgcc gaccggacgc gagatccggc agatcgtcgt
    10201 cctcccggag taaccttctt tcggccttcc gttcgagacc cttccacgcc cgccgtccgc
    10261 ccactatcct cccttgatgg gacccgagcc gccgttcgca atgagcgtgg acgtggagga
    10321 ctacttccag gtgcaggcgt tcgcccacca cgccccgcga gacggatggg accgctggcc
    10381 ctcacgcgtg gaggggaaca cacagcgact cctcgatctc ttcgatgaag ccggcgcgac
    10441 cgccacgttc ttcgtgctcg gctgggtcgc gcgccggcat ccttcgctcg tgcggcagat
    10501 cgcgtcccgc ggccacgagg tggcgtccca cggcatggag caccggatgc tgaccgagca
    10561 gtcgcccgaa tccttcgccg ccgatgctcg cgattcgaag gtgctgctcg aagacgtatc
    10621 gggcgctccc gtcctgggct ttcgcgcgcc gagctacagc atcggccgat ccaccctgtg
    10681 ggccgtcgac gtgctcgcgg acgctggata cgcgtacgac tccagcgtct atccgatccg
    10741 acgccagagg tacggttacc cggaagggcc ggaccggccc gcgatgatcc acggtccaag
    10801 gcgggccctt ccggagttcc cgctccctac gctcggcctc ggttcctggc gcgtcccggt
    10861 gctggcgggc gcctacctgc gcctctggcc gtcctggatc tcgcgcttcg cggcggggga
    10921 gcatctgaga cggcggcgac cgctcgtcgt gaacgtccat ccctgggaac tggacccgga
    10981 ccagccgacg atcggcggcg cgcgctggcg cacctggacc cactacaccg gtcttggccg
    11041 cacccaatcc gtgctgcgcg ccgttctctc tcgcgcgcgc ttccgcgaca tcgccacccg
    11101 cctccgtgag cttgggctgc tcgacggtcg catcgcccgg gcgggggagg cggcctcgtg
    11161 aacctggacg tcctgatcct ggtcgcgggg ggcgtgatcg cgatcctctc cgccgcgctc
    11221 gtcctcgccc tcagccccgc ccgctatccg tcgctcgtcg ccgccggctc cttggctgcg
    11281 ctcggcgtgc tccaattcgg gtgggctcgc gcggcatcgc cgttcatggg aggcgagaac
    11341 cgcatctggt tcgagctctc cctcgccttt gcggtcgtcg tatccctcct ctggctcctg
    11401 ctttcgcgca ccatcggact gggcccgcgc cccagtccga tcggaccatg gcgctggtac
    11461 atcctggccc agtccctgtg cgccgcggcg gcggtcgctc ggctcgcgct cgagccgacc
    11521 tacgaggaaa tgcagcagat cggcggggga ttcgcctacg gtctcggccc attcacgacg
    11581 cttctcatcg tcgcgcagct cctcaacctg atcctgctca cggcgagctt cgagtcgacg
    11641 tacttcgcct tgcccgcctc accgcgccgc gcgttcctgc ccggcctcat cgggatcctc
    11701 gcgtggaccg gatatttcac ctacgtctgg atccagagcc tgatcctggg cagcgtgcac
    11761 gtgggagacc tgagcacggg cgcgttcgcg gtcatggaca tcgcggtgct cctgccgatc
    11821 tcgctcatcc ggggccgtct cggcgaggcg cacgtgcgcc gggaaccccg gccgctcgcg
    11881 cgcaccatgt cggtgggatt caccctcgcc ttcctgctgg cgacgaccgg actcctctgg
    11941 atcgcgcacg cgacgggatg gagtctggcg cggagcgtct ggatcgtgct cggcctcctg
    12001 ggcgcgctcg ggctcgccgc gctcatgatc tcgaaccgag tgcagcggcg cgtgcagcgc
    12061 tggatcgacc cctatctcta tggcggcgtg ctcgaccgga ccgggatcga gcgacgcgcc
    12121 gatgagatgc tggccgtctc acgctccgcg gcggacgtgc acggcgtcat tccgtccacg
    12181 accgaagaga tcgtcggcgc gcgcccggtc acgctcttcc tcgcgagcag cgacggaacg
    12241 cgcttcattg tcgctgcgag cacgatcgct cccgcgccgc cggtgtcggt ggatgtggaa
    12301 gcgccgctcg cgatcgagct ccgtcgcgca ggacgcgcca tccgtttgcg cggccgcccg
    12361 gacgacctcg agtacattcc gatttacgtc gagaacgcgg gccagctcga ggcgtgcgac
    12421 gcggtctgcg ccgcgccgct cctggtcgac gaggagatgg cgggattcct cttgtgcggg
    12481 gaacggggaa gcgatccgac ctcccggcgg cagctcttgc ccctcctgga cctgctttgc
    12541 gcccgctatt ccgaggctct cacccgggtg tcgttcaggg ccgccagttc cgagcccaga
    12601 gctgtcgatg tctaacacca ttacccacca gtaagttagc ctaaacacct cgcccaaacc
    12661 gtgcatactg caaatattct ttgacacttt cctgggcgat cgtgtatcat acttttcgtt
    12721 cggggattta cgggcgaaag ggctcaaaga aagtggttga ccgaaccccc gtccggcaag
    12781 tattattgac agcgttctcg gaagtgtggc cgggaactcc aacgcaatta tccatgtgaa
    12841 acggatgtcc tcatcgtggg agaggtgcag ccggtgatct gccgctcgcg caacatcatc
    12901 ttcggattga tcgcagcgac actgttcgct gcaaacacgg cgcacgccat tcccgcgctc
    12961 cagctgtaca gccccgacgc catctacgac gtttcgtccg cctcgtggat catcaacaag
    13021 tccacgttcg agctctacgt cattggcgac gtggagaagg tcgggacgat ctacgacgtc
    13081 gacctcgtcg cctccgtgta cggaagcggc ggcaacatca ccatcacgcc gaacctcgcg
    13141 atcgacagca cgcccgagga cgccgcggcg tacagcaatg tgatcaacca tgccgagtaa
    13201 aagaacgccg atgcgcacta cttctaccat ctcggcgact tcgatcagac gagcgacctc
    13261 atctacgact acacgggcag cagcggcggc acggaccacg gccagatcct cgctctgacc
    13321 gtgagcgtgt cgggctacga tgcggtccac ttcgacgcgt tcgatcacta catgacgggt
    13381 ggtaacggcg gcggctcgca ggcgtccttc ctgacgcgtc cgcagggcgt gttcgctccg
    13441 ttctcgcacg acgcgaccga ggggaacccg cccggcgcac ccgaacccgg ctcgatgctc
    13501 ctcgtcggcc tgggactggg agtggctgcg ctgaccggac ggcgcaagaa gagctgagcg
    13561 acttcaagat cgcacgaggc ctggacgatc caaggatcgt ccaggccttt tcgcttccta
    13621 gcggcttgac accctcccgc aagcttctct aaggtggtcc gcacatgact gcgaagcgcg
    13681 ttcgtggccc ggtggttcta gccctactgc tcttgatcgc tgcgccggct atggcgcagt
    13741 cgcctccagg ctcgcagccc acgacgtccc cacagaatcc gccgaccaat ccagacgcgg
    13801 tcaagcctcc gcctcctccg atcctcacgc tcttcacggt tccgcgcgac gccgacgtcg
    13861 tcctgaaggg acgcaccgac ctcgccgggc ggactccgct cgatttcccg gtgaccgtgg
    13921 aagggaagta ctccgtgcgg ctccggggca ccggctatcc cccggtgcac ggcgtcatct
    13981 acattccgcc gcgtgggcag ctcgcgttcg tcctctccga gcctcgcgaa ggatcgcttg
    14041 ggctcttcat gcggggcctc aactatccgg gcgtgcctca gctcgggacc ggacgcacgt
    14101 cgcgcggcgt cacgctggtg acctatgcga gcggcggaat cttcatggcg gccaaggcgc
    14161 actcggacta ccgcgagcgt ctggacgagg tcggcgagtt cccggaggac cgtgctcgcg
    14221 acgagcgacg gtaccggaat ggatggctca tcttcacggg cgcgacctgg gcgatgagcg
    14281 cgctcgacta ctggacgcgt ccccggttcg acctcgccga gaccaccccg tcgcggctca
    14341 cgctggacgt gcctacgatc acgcgagtcg gcgccgtctg gcgctcgctc gtcgtgcctg
    14401 ggggagggca ggatttcgcc aaccacagga cgcgtggaac cgtgtggctc gctacggtca
    14461 tcgccgcggg ggccggctat gtggtcacgg actaccgcgt gaaccgcgac gagacggatg
    14521 tgaaatgggc caagatcgca gtgaacgatg cgggacccgg caccatcgac cagaagctcc
    14581 tcgagctcga gcagcaggag cgcgatctct ccgcgtcgca ggacgcgcgc cgaggcttcg
    14641 ccatcgcggg catcgccctc tactcgctca acctgctcga ctcgatgatc atgccgctcg
    14701 gctacaaccc gcccaagcgc gccaaggtcg cctccatcga gccgatcgtc gatcccagaa
    14761 tggcggggat gtccgtcaac gtccgcttct agtcctcgcg tttagtctcc gcgtccagat
    14821 ccgcccctag cactcccggc ccaccgtcca atttcgttca ggcgcgttca gtcgcgtcca
    14881 cacccgcgtc tatcgtcttg cgcgccgcct gtcgcaattc gcgattcgag tgctccaacg
    14941 taggcgtgca aaacgccgtg aaactgcgtt cgttcctacc cgcttggagg gggcgcggat
    15001 ggagcctccc tggtcgtgca ttttgcgtga cccccctggc acgctccctg cagaaccagc
    15061 gaaagccctg gcactacgat ggatcgtcac ctcgccgggg cctcgtgtcg gaacaacatc
    15121 tagggggtaa ccaccatgca gaggcccctt cccgcgtggg gagcagcgta tagccccctc
    15181 cggatcgccc tcgtggctgc cgcggtcgcg cttggcctca tgtacgcaga cgtacgctcg
    15241 tcgtacgccg cagtcgctgt tggcggtagc acgggtgact tcctgcagtt cgaagtcgga
    15301 ggccgcgcgt ccggaatggg cggcgcgcag gtcagctccg cctccggcgt catggcccag
    15361 ttctggaatc ccgcgggcct ggcgtcgctc gagcggcccc aggtgggggg catgcacgcg
    15421 acctggctcg acaacctgag ctatgaatgg ctcgggtacg cgacgcccgt caatccgcgc
    15481 ttcggcgtcg gctccgtcag tctcgcctac ttccatatgc cgagcatcca gggagccgac
    15541 gagttcggca atccgaccgg cgacttccgt gtctacgaca tggccttgac cgccgggctg
    15601 gcgaagccgg tcgggcgtca tctctccgtc ggcgggaatg cgaagatgat ccggcagaac
    15661 ctggcgacgg tctccgcgac cgggttcgcg gtcgatctgg gcgcgtccta catgacgcac
    15721 ggtgccacgc tcgccgccgt cgtccagaat ctgggacccg agctctcgta cggcaccggc
    15781 ggttcgtatc cgctctctcg ccagatccgg tttggtttga gccgcgagtg gctctcgggg
    15841 aagctcctcc tcggcgccga ctacaacatg ccgagcgact actacgacga cgctcgattt
    15901 ggcgccgagt accgccttca tcccaacgtg tccatgcgga tgggataccg ccatcaattc
    15961 ggtgagctcg acgatccggc cgaggggatc tcctacgggc tcggcgtgca cttcggccag
    16021 ctcaacgtcg attacgcgct cacgccggac aacgacttct cggacgtgca ccggctctcg
    16081 ttcggctact ccttcggctc gagcgcgaaa gaggcggagc cgaagcagcc gaagcacgag
    16141 gagcccaagc ctgagccgcc ggccccgacc gggccgaagg tgatcgccca ggctccggcg
    16201 cccaagccgg caccagcacc caagccaacg ccggcgcaga ccaagcccgc gccgcaggca
    16261 gacgctccca agaccgagat cgtcgccctg gcgcccgccg cgaagccgga ggccgaggct
    16321 ccgaaggccg aggcggtcgc acccccgccg gcgcccgcgg tcgccaccca ggcgccctcg
    16381 aaggagcctg aagccaagcc cgctccggca ggggaagtcg tgtacctggt cgtcctgccg
    16441 gggtacccgt ccaaggagag cgcccaggcc gagatgaagg ccctggagct cctgggcttc
    16501 cgcaccaagg acgcccagat cgcgagggac ccgaagaagg gcggctaccg aatcgctttc
    16561 gtcaagctca agaacaaggg gaacgccgag gaggtggcct ccgacctcca gcggatgtcc
    16621 ttccgggcct tggtcgaggt agcacagaag tagtagtaga cacacgctct tgcgcccgcc
    16681 gtcagcctct gctatcatta catctgttgg tggggggcgc gtgatatccg cggctggttc
    16741 tgcatcgccc gcgtagtccc gcagcagtct ccatcgccaa ccccattcgg tgccggtgcc
    16801 cgatggtggt tgcgcctagt acccggtcac ctcgtgggga ggtgtctctc cctgcgtttt
    16861 gactgggccc caccggcgcg acatcactaa cgacgcggcg gacgatgctc ctaccagcgg
    16921 aactcaagaa tcgggatcag gacggcctgt ccacggccga cgtgctctat tcttggttca
    16981 agcgcagcct cgacatcctc ctctcctcgg tgggcctcgt ggtcatggcc cccgtgttcg
    17041 ccgtgatcgc cgccgtcatc aaggtcgaca gcaggggccc cgtgctctac ggacaggagc
    17101 gcatcgggat caaccgccgc cgctcggacg gcacgcgccg cagcacggac gagcgccgga
    17161 agcgggacac gttcggccgc ccgttcaaga tctggaaatt ccgcaccatg atcgtggacg
    17221 cggagaa
//
LOCUS       DASTKC010000221        17596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1607975, whole genome shotgun sequence.
ACCESSION   DASTKC010000221 DASTKC010000000
VERSION     DASTKC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17596
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607975"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFR25_09235"
     CDS             complement(<1..140)
                     /locus_tag="VFR25_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011477723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9941277.1"
                     /translation="MAKQKYERNKPHVNVGTIGHVDHGKTTLTAAITMVLAQNNPKIA
                     VR"
     gene            complement(236..311)
                     /locus_tag="VFR25_09240"
     tRNA            complement(236..311)
                     /locus_tag="VFR25_09240"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(276..278),aa:Thr,seq:ggt)
     gene            complement(712..787)
                     /locus_tag="VFR25_09245"
     tRNA            complement(712..787)
                     /locus_tag="VFR25_09245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(752..754),aa:Gly,seq:tcc)
     gene            complement(860..941)
                     /locus_tag="VFR25_09250"
     tRNA            complement(860..941)
                     /locus_tag="VFR25_09250"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(905..907),aa:Tyr,seq:gta)
     gene            complement(956..1030)
                     /locus_tag="VFR25_09255"
     tRNA            complement(956..1030)
                     /locus_tag="VFR25_09255"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(996..998),aa:Thr,seq:tgt)
     gene            complement(1104..3236)
                     /locus_tag="VFR25_09260"
     CDS             complement(1104..3236)
                     /locus_tag="VFR25_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941278.1"
                     /translation="MALGPGPRTLKAELQFRFLVVGSVFLVVATALLYTNGQGSLRRQ
                     VLTNAVTASETAASLIAVEDHRLIRTAADMNGQAFRTIVTNLGALRRANPSVYHLFTL
                     APGKQLGRWGVVVDLGSTGNESGAPDPALVRGRLPIGSPPPKDVPESLLRQGMTQTAG
                     VVLDLTRPDLARVVAVSPIRTTGGESVGLVVVQMSAAALASEARLLAYVCVGIFLLGL
                     VASAFASTYVSRWLTRPIEDLLGAVEAISKGDLATRVRVDGHKHELDALGSAFNHMAE
                     TLESSRGRNDAQQGRLRELNRIGTSAAATLDLPLMLQTVAEGMRTVCGGQEAFVGVAA
                     PRDRAIHHWAGSDPGTVDLMTTPVQLEDLTRVLGGEARLLSRAEMDAAGLSWLRQRPG
                     EYALAAPLKASDETIGALIALGDRAQFHDDAVSLASVFATQISAAVGNARLFEQIKAL
                     DRSKSEFLSIASHEVRTPLTVIKSSLDVLVANKAFTYTTDQRQLIAFCQQSVERLIQL
                     VKDILDVSKIEAGVLQIQFIPTSLNELVTKCLFWVPQLPGGQGIEVEARLPSEPAMVS
                     ADPGRIMQVLENLISNALKFSKPGGKVTIELVEHEAEYEIVVTDQGKGIAAEHLERIF
                     GKFYQVEESATREQGGTGLGLAICKGIIQAHRGRIWAESELGKGSRFHFTLARYQDGA
                     DAGRPDAQMSVADLLSSLRSETPSPTRS"
     gene            complement(3262..4677)
                     /gene="cysS"
                     /locus_tag="VFR25_09265"
     CDS             complement(3262..4677)
                     /gene="cysS"
                     /locus_tag="VFR25_09265"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9941279.1"
                     /translation="MSLRVYDARRRAKIPFESAKPGKVGMYVCGMTVQDRPHVGHMRY
                     AVAGDVIRRILESRGYEVTYATNFTDIDDRIIERGNKEGVPFEQVSERNIKAFLTYAA
                     LLNIKPATVYPRATEHIPEILALIQRLIDSGHAYPAGGDVYFDVRSYPAYGSLSGRKV
                     DDLRSGVRIEVGEAKRDPLDFTLWKGAKPGEPSWPSPWGPGRPGWHIECSAMAMKHLG
                     ETFDFHGGGQDLIFPHHENEIAQSEAATGKTFARFWVQNGLVNLDGEKMSKSTKHFFL
                     IEDVAKEVEPEVIRFYLQSTQYRSPIEWNDVRLREAGAAYDRLREALAKAEAAPAADG
                     TSTAGSTLLAELAELEKEFQESLDDDFNAAKAQGHLFEMAKAINRVADSAQSSVADRA
                     ALPEAGRTLRRLGELIGLFWGSGKVEIEAPEAVQALVRQRDEARIQKQWRRADELRDE
                     ILALGYVLEDSKGGTKVRPNH"
     gene            complement(4674..5588)
                     /locus_tag="VFR25_09270"
     CDS             complement(4674..5588)
                     /locus_tag="VFR25_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET9941280.1"
                     /translation="MNPVRHTASPEQAFTLVEILVRIGIIILATLVTVRLVYLLIGRI
                     ERAIRSEAKGTELAREKRARTIGEVLRGFSRGIIIVIGILMCVRELGLDITPALAAAG
                     GFGVAAGLGAQSVVKDWIAGFFIIHDNQFAVGDVVRTMGVSGTVETLTLRHTELRDGE
                     GFIHFISNGEIKVVTNLTKSWSTPIVRIPISLTEDPARVVQVLEAMLVRFAASPNAKP
                     LLLEPPRLLGIDDVGTGQFTIMLQAKTVPEERLAVSRMLRMAALESLRAAGVLVHVPV
                     AGDAPAAVQQAAPADAPAQAATSSGDAA"
     gene            complement(5619..6662)
                     /locus_tag="VFR25_09275"
     CDS             complement(5619..6662)
                     /locus_tag="VFR25_09275"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET9941281.1"
                     /translation="MKIVVLLGGNTSEREVSLRTGAGVARALQTLGHDVALLDTGTGQ
                     FLKDPDHAMALGASPAPLHAAGGLPARRGEQGLDVYVRSIPRTADLVFIALHGGAGEN
                     GTLQALLDLAGIPYTGSGVLSSALAMDKVMSKRIFKVEGIPTPEWVERWAPEDPAAAW
                     HPEITAEELARLGGYPLIVKPNEEGSSVGITVVKSASEWKPAMEEARRYGRLVLVEQF
                     IDGRELTVGVIGQRALPVVEIIPTAGWYDYEHKYTSGASRYECPASLPPEVAEELFAL
                     SLRACRALRTSGVARVDFRLGKDGVAHCLEVNTVPGLTELSLVPKAAQAAGLRYEDLV
                     KAIVESAPVRAAR"
     gene            complement(6676..7170)
                     /gene="ispF"
                     /locus_tag="VFR25_09280"
     CDS             complement(6676..7170)
                     /gene="ispF"
                     /locus_tag="VFR25_09280"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET9941282.1"
                     /translation="MTSGTTLRVGIGYDVHRLERGLPLTLGGVAIAHDKGLRAHSDGD
                     VLAHAIGDALLGAAGLGDLGAHFPDSDPRWRGASSLRLLETIAEQLRERGYTIVNVDA
                     SLVAEAPRIAPHVAAMREALGLALGIGEGRVSVKATTNEGLGALGREEGIAAWAVALV
                     EGRE"
     gene            complement(7167..7877)
                     /gene="ispD"
                     /locus_tag="VFR25_09285"
     CDS             complement(7167..7877)
                     /gene="ispD"
                     /locus_tag="VFR25_09285"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET9941283.1"
                     /translation="MSITSRRDLAVVLAGAGDGVRMGGLGPKLLLQIAGKTLLERVAS
                     IFLAHPSVGELIAVVPEALLEPARRALSALPASSDIRREAAPGGATRRESVGRGIRAL
                     TLGLPYVAVHDVARALVEERLITRVLETARLTGAAIPAVPLRDTVAEVDAGRVVRTLD
                     RARLFGVQTPQIFADAILRRAHASAEAEGRDATDDASLLEASGAKVAVVPGDPMNLKL
                     TEPSDLAMFEFLLRRGSP"
     gene            complement(7874..9280)
                     /gene="radA"
                     /locus_tag="VFR25_09290"
     CDS             complement(7874..9280)
                     /gene="radA"
                     /locus_tag="VFR25_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET9941284.1"
                     /translation="MTPRKATPAREAKTVFFCAECGNESPKWFGRCPHCDAWNSAREG
                     PGRREAAPARRSNYASPDVASAPLRLDEIDSGAEPRWVSGIGELDRVLGGGLVPGSLV
                     LIGGDPGIGKSTLALQLAAALGRADRSVLYVSGEESPRQVKLRAERLALRGGLDRLWM
                     QSEIDLERILEEVARKNPSLLVIDSIQTLYLGSLDAAPGSVSQVRECGLRLLRLAKER
                     NLPVLLIGHVTKDGSVAGPRTLEHMVDAVLYLEGERHHEYRILGAAKNRFGSTHEIGV
                     FTMQGDGLAEVENPSERLLKDRGESVPGSAVVATIEGSRPLLVEVQALVAPTHFANPQ
                     RVAQGIDARRLAVVIAVLEKRAGLSLAGADVFVNVAGGIRLEEPAADLGLALALASSF
                     RDRPLPPDLVAIGEVGLGGELRPVPQLERRLAEARRLGFRAAIVPKRSAPEGVTGALG
                     AASLHEAIGRVLDPDHAR"
     gene            complement(9313..10212)
                     /locus_tag="VFR25_09295"
     CDS             complement(9313..10212)
                     /locus_tag="VFR25_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9941285.1"
                     /translation="MNRTSRRAAIQVGVAGLLSILLLFIGIAWIKEYRVGKRKSYYTA
                     RFEEVGNLGEGDPVSVRGVRKGAVTNIVLEDQGVRVEFEVDKGVTLHPDAQLRVANIG
                     FMGEKFLALDPGQAPGRYDGRKPIPGRFQSGVPEVISGAGDLLIETTELSSRLNLMLD
                     AIDPATVERASKNFEKASEGLNQTLNQNQGDLRQAIVDFRAAAHDLRAIASANGGQVS
                     SSVKDFGEASRKLSHLSDQLSTTAVSLDRVVTRLDKGEGALGKAISDSTLYVDLKETL
                     RNTNQLVKDIQKNPKKYINLSVF"
     gene            complement(10223..10924)
                     /locus_tag="VFR25_09300"
     CDS             complement(10223..10924)
                     /locus_tag="VFR25_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9941286.1"
                     /translation="MLRGVDLSVSRGECLVVIGRSGTGKSVLLKHVVGLLQPDRGTVR
                     VNGIDVAKLDEGELLALREQMGMLFQGGALFDSMLVGENVGLALREHTPLPERQIEEV
                     VAEKLALVGLTGTEGQRPSSLSGGMKKRAALARALALNPKIMVYDEPTTGLDPITADV
                     INRLISRLQERLGMTSIAVTHDMRSAYHIADRIAMLHEGRIHAIGTPEEVQSTRDPIL
                     RQFIEGSSDGPLLPT"
     gene            complement(10974..11795)
                     /locus_tag="VFR25_09305"
     CDS             complement(10974..11795)
                     /locus_tag="VFR25_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9941287.1"
                     /translation="MIDPETAEQEDAALAAAPWPQRFLFARLQNAGEIFLLVGSALRE
                     MPAGVRKIGLVFEQMVSIGLGSMPLTVIVALFTGAVAAVQAAYQMRDYVPMIYLGTVI
                     GKSVVIELGPVLTALVVGGRVSAAIAAELGSMRVTEQIDAMETLGISPVRYLVVPRLV
                     ASVLMLPMLTIFADILAIFGGYLVAVLTLDVSGHTFTSGLKLYFKIQDVFSGLLKSVF
                     FGGIIATMGCYYGLRTEGGAEAVGDSTTRAVVASCLLILIVDYLLASFLFRVLFA"
     gene            complement(11792..11980)
                     /locus_tag="VFR25_09310"
     CDS             complement(11792..11980)
                     /locus_tag="VFR25_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HET9941288.1"
                     /translation="MVLFVYRPEVYETDPSKMDGKAEIIIAKQRNGPTGSVDLAFLRE
                     CTRFESLRDVPEPRPEPR"
     gene            complement(12058..13170)
                     /gene="dnaB"
                     /locus_tag="VFR25_09315"
                     /pseudo
     CDS             complement(12058..13170)
                     /gene="dnaB"
                     /locus_tag="VFR25_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
     gene            complement(13185..14051)
                     /locus_tag="VFR25_09320"
     CDS             complement(13185..14051)
                     /locus_tag="VFR25_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9941289.1"
                     /translation="MDDGRGELVAIAGEAVTLLRRELARGRRKIELPGIDGEAPMPAA
                     PRGAASASRSPEAPVASAPTAGATPLTLPGFEPPAPPLSAPWSAPMPAPVRLAPRSDL
                     PGTLAEVKALVDTCRLCPLCETRTRTVFSDGTDKARLMFVGEAPGRDEDLQGVPFVGR
                     AGQLLTKMIEAIQMKRADVYIANVLKCRPPENRTPIPSEVEKCLPYLEQQIALVRPDL
                     IVALGLSAAQGLLGTKASMGALRGRMFEYKGVPLVATYHPAALLRNPGLKKDAWVDLQ
                     RVRDLLREGAPA"
     gene            complement(14062..15360)
                     /gene="coaBC"
                     /locus_tag="VFR25_09325"
     CDS             complement(14062..15360)
                     /gene="coaBC"
                     /locus_tag="VFR25_09325"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET9941290.1"
                     /translation="MTETRSLAGARVLLGITGGVAAYKTPELVRALAKEGAHVSVILT
                     TRARRFVTKDALRSVTKGPVLHGLFETQALGSGPWFEGMPESALGMAHIGMAREADLI
                     LVAPATANAIGKLAHGLADDLLSTALLATRSPVLLAPAMNVAMWEHPAVQANLDVLRA
                     RGIRMVGPEEGSLADGEWGMGRMASLEAIVAACASLVGLAGAGRGAGGDGSGANGLPL
                     HGRTIVVTAGGTEEPIDPVRVITNRSSGKMGFAIAAMARDLGARVRLITARVTAEPPA
                     GVERIDAPTAASMADAVLRAVKDADALVMAAAVSDFAPTKPSGSKLPREGSGLKLDLA
                     PTTDILKEVRAAHPGKLLVGFALETEDEINRGRGKMQRKGLDLVAINNPGKPGSAFGS
                     DTNDVTLIDSDGRVEALGLRPKREVARAILLRVAKALNQD"
     gene            complement(15367..15603)
                     /gene="rpoZ"
                     /locus_tag="VFR25_09330"
     CDS             complement(15367..15603)
                     /gene="rpoZ"
                     /locus_tag="VFR25_09330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET9941291.1"
                     /translation="MDSKTPLRSSNVPNKYELIIVAAKEARRINELSRQTGLNLGSKV
                     TNVALEEALKGNVLYHYEETPAEPEEAPSADSEE"
     gene            complement(15616..16230)
                     /gene="gmk"
                     /locus_tag="VFR25_09335"
     CDS             complement(15616..16230)
                     /gene="gmk"
                     /locus_tag="VFR25_09335"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET9941292.1"
                     /translation="MIGSEYRRFVVVISGPSGAGKSSFVKVLLGRYPRDLVYSVSATT
                     RPRRAHEVEGRDYFYLGREEFQRRVHAGDFVEHAEVHGELYGTLKSQTEAVLRSGRNV
                     LLDIDVQGGRSVRRIYPDGVFIFVLPPSLADLEERLRARGTDSEERIKLRLENARREI
                     EIAREYDYAVVNDDLEEATRRVLAIIEAETCRASRRIDGGRSEL"
     gene            complement(16238..17131)
                     /locus_tag="VFR25_09340"
     CDS             complement(16238..17131)
                     /locus_tag="VFR25_09340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET9941293.1"
                     /translation="MIRSMTGYGRGEVVVDGLRLAAEVRSVNHRFCELSARLPRTLGI
                     YEHEARKIVQERSVRGKINLVVTWGEEGGDDGREPTGTLTLDERAADRYMELLQALQR
                     KYSLSGSVDVDTFASLPNLFVWSEMRRDPEFYLGILREVVTAATEDMIRMKETEGAAL
                     RTDLEARVEGIRDRVAQIRLRAPQKVKETAEKLQDRIRVLLDGNELPEERLAQEVAMM
                     ADRLDATEECVRLEAHCDHFRKLLNEEATPGRKLGFLLQEMNREINTIGSKSADVPII
                     EQVVDVKEELERIREQVQNIE"
     gene            17283..>17596
                     /locus_tag="VFR25_09345"
     CDS             17283..>17596
                     /locus_tag="VFR25_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941294.1"
                     /translation="MAAGGNRASGPAAGAARARAGAPRWRAWVLGAVVALAALLPHAR
                     SIGYDFVWDDRYLVGEHLDIHGPADLARLWSTPFDVYLQTQTSQHTYFRPATVYSLAL
                     DRA"
BASE COUNT         2724 a         6106 c         5930 g         2836 t
ORIGIN      
        1 tcccgaaccg cgatcttcgg attgttctgc gccaacacca tcgtgatcgc cgccgtcaac
       61 gtcgtcttcc catgatccac gtgcccaatc gtccccacgt tcacatgcgg cttgttccgc
      121 tcgtatttct gcttcgccat cagaccagat cctccgtaca ggacttcgcc ggaatcacgc
      181 gacgggcgag atcaagaccg tcggaacgcg ctcgacctct cgcgacagat ggaaatggag
      241 cccacgagcg gaatcgaacc gctgaccttt tgcttaccat gcaaatgctc taccgactga
      301 gctacgtggg cggaggtgag gcgcgcggaa cgacccgaag cgcgaccgaa tgccgacccg
      361 gttcacttcc gagccgacac cgctcggcgc caaacgctca atacaaggca aaaaacgccg
      421 accggccctc ctcactccat cgaagaaggt cgtcgtcggt gcgaatcgca aaaggtacgg
      481 acccgcgaac ccgcggtcaa caagaaattt gactcatctt gtggccgttt gattcaggtc
      541 cgatttgggc ccgctcggga cggaattgtg ccccgaggag ccgcgtacca ccacgcaatc
      601 cacaacgaaa cggttacggc cccggcgccg cggtgggtcg gccgggttta tcttctgcag
      661 cgcaatgggt tgaggaaagt ggatggaggt ggataactgc cgtagggaaa ctggagcggg
      721 aaacgggatt cgaacccgcg accctcagct tggaaggctg acgctctagc cactgagcta
      781 ttcccgcccc gtttcatcac ttggggctaa cggccccact ccaaggggcc gatccgctct
      841 cacttcgatg ccgaaagaat ggtgggggaa ggattcgaac cttcgaaggc taagccggca
      901 gatttacagt ctgctccctt tgaccgctcg ggaaccccac cgggatgacg tcacttggag
      961 ctggcgaagg gactcgaacc cccaaccgcc tcattacaaa tgagatgctc taccattgag
     1021 ctacgccagc tacgcggggc ggggggcccg ggccccggtc cgctcggcgg cagtgtacgg
     1081 gcggggcgag cccgccgtca acgctacgag cgtgtcggtg aaggagtttc ggagcggagg
     1141 gaggagagga ggtcggccac ggacatctgg gcgtccggac gtccggcgtc cgcaccatcc
     1201 tgatagcggg cgagcgtgaa gtggaagcgg cttcctttgc ccagctcgct ctcggcccag
     1261 atgcggccgc ggtgcgcctg gatgatgccc ttgcagatcg cgaggccgag tccggtcccg
     1321 ccctgctcgc gggtcgccga ctcctcgacc tggtagaact tcccgaagat ccgctcgaga
     1381 tgctcggccg cgatcccctt cccctggtcg gtgacgacga tctcgtactc ggcctcgtgc
     1441 tccacgagct cgatcgtgac cttgcctccc ggcttggaga atttgagcgc gttcgagatc
     1501 agattctcga gcacctgcat gatgcgcccc ggatccgcgg agaccatggc cggctcggac
     1561 ggaaggcgcg cctccacctc gatcccctgc ccgccgggta gctgcggcac ccagaagagg
     1621 cacttcgtca cgagctcgtt gagcgaggtc gggatgaact ggatctgaag aacgccggcc
     1681 tcgatcttcg acacgtcgag gatgtccttc acgagctgga tgagccgctc gaccgactgc
     1741 tggcaaaagg cgatcagctg gcgctggtcg gtcgtgtagg tgaaggcctt gttcgcgacg
     1801 agcacgtcga gcgagctctt gatgacggtg aggggcgtcc gcacctcgtg cgacgcgatc
     1861 gagaggaact cgctcttcga gcgatcgagc gctttgatct gctcgaagag ccgcgcgttc
     1921 cccacggcgg ccgagatctg ggtcgcgaac accgacgcga gggagacggc gtcgtcgtgg
     1981 aactgcgcgc gatccccgag cgcgatcaag gccccgatcg tctcgtcgct cgccttgagc
     2041 ggcgctgcga gcgcgtactc gccgggccgc tgccggagcc acgagagccc ggccgcgtcc
     2101 atctccgcgc gggacaggag ccgcgcctcg ccccccagga cgcgcgtcag gtcctcgagc
     2161 tggaccggcg tggtcatgag atccaccgtg cccggatccg atccggccca gtgatggatc
     2221 gcccgatccc gcggagcggc gacgccgacg aaggcttcct gtccgccgca cacggtgcgc
     2281 atcccctcgg ccaccgtttg gagcatgagc gggagatcga gcgtggcggc cgcgctcgtc
     2341 ccgatccggt tgagctcgcg aagccgcccc tgctgcgcat catttcgacc gcgcgacgac
     2401 tcgagcgtct cggccatgtg gttgaacgcc gacccgagcg cgtcgagctc gtgcttgtgc
     2461 ccgtccacgc ggactcgcgt ggcgagatcc cctttcgaga tcgcctcgac cgcgcccagg
     2521 agatcttcga tcggccgcgt gagccaccgt gagacatagg tgctcgcgaa cgcgcttgcg
     2581 acgagaccca ggaggaagat tcccacgcac acgtaggcga ggagccgcgc ctccgacgcg
     2641 agcgccgccg cgctcatctg cacgaccacg agaccgaccg actcgccccc agtcgtgcgg
     2701 atgggagaga cggccacgac ccgagccagg tcgggacgcg tgaggtcgag caccaccccg
     2761 gcggtctgcg tcatgccctg gcggagcagg gactccggca cgtccttcgg cggcggcgat
     2821 ccgatcggaa ggcgcccgcg tacgagcgcc gggtcggggg cccccgactc gttcccggtg
     2881 ctcccgagat cgaccacgac tccccacctg ccgagctgct tcccgggcgc gagcgtgaag
     2941 aggtgataga cggacggatt cgcgcggcgg agcgcgccca ggttggtgac gatggtgcgg
     3001 aaggcctgac cgttcatgtc cgccgcggtg cggatcaggc gatggtcctc gaccgcgatg
     3061 agagaggccg cggtctcgga cgcggtcacg gcgttggtga gaacctggcg gcggagggag
     3121 ccctgtccgt tcgtgtagag gagcgcggtc gcaacgacga ggaacacgct ccccacaacg
     3181 aggaatcgga attgaagctc ggctttgagg gtccgcggac ccggtcccag agccatcgac
     3241 ccgccgtgaa cttggtaaaa atcagtggtt gggccggact ttcgtgcccc ctttcgaatc
     3301 ctcgaggaca tagcccagcg ccaggatttc gtccctcagt tcatcggcac gccgccactg
     3361 cttctggata cgggcctcat ctcgttgccg cacaagcgct tggacggcct ccggagcctc
     3421 gatttcgacc ttcccggaac cccagaagag accgatcaat tcgccgagcc ggcggagcgt
     3481 gcgtccggct tccgggagtg ccgcacgatc cgcgaccgac gactgcgcgc tatccgcgac
     3541 gcggttgatc gctttcgcca tctcgaagag gtggccttgc gctttcgccg cgttgaagtc
     3601 gtcgtcgagc gactcctgga attccttctc gagctcggcc agctcggcga ggagcgtcga
     3661 gcctgccgtg gaggtgccgt cggcggcggg agcggcctcg gccttcgcga gcgcttcgcg
     3721 caggcggtcg tacgccgccc cggcctcgcg gaggcgcacg tcgttccact cgatcggact
     3781 ccggtactgc gtcgactgga ggtagaagcg gatcacctcc ggctcgacct ccttcgcgac
     3841 gtcctcgatc aggaagaagt gcttggtcga cttcgacatc ttctccccgt ccagattcac
     3901 gaggccgttc tggacccaga agcgcgcgaa ggtcttcccc gtcgcggcct ccgattgcgc
     3961 gatctcgttc tcgtggtgcg ggaagatgag atcctgcccg ccgccgtgga agtcgaacgt
     4021 ctcgcccagg tgcttcatgg ccatcgccga gcactcgatg tgccatccgg ggcgccccgg
     4081 cccccagggg ctcggccacg acggctcgcc cggcttcgct cccttccaga gggtgaagtc
     4141 gagcgggtcc cgcttcgctt cgcccacctc gatccggacg ccggagcgaa ggtcgtccac
     4201 cttgcgcccc gagagcgatc cgtacgcggg gtagctccgc acgtcgaagt agacgtcccc
     4261 acccgccggg taggcatgcc ccgaatcgat gaggcgctgg atgagcgcga ggatctccgg
     4321 gatgtgctcc gtcgcgcgcg gatagacggt cgccggcttg atgttgagga gcgccgcata
     4381 cgtgaggaag gctttgatgt tccgctccga gacctgctcg aatggaacgc cctccttgtt
     4441 cccgcgctcg atgatgcggt cgtcgatgtc ggtgaagttg gtggcgtacg tcacctcgta
     4501 gccgcgcgac tcgaggatgc ggcggatcac gtcgccggcc acggcgtagc gcatgtggcc
     4561 cacgtgcggc cggtcctgga cggtcatgcc gcacacgtac atgccgacct tgcccggttt
     4621 ggcggactcg aacgggatct tcgcgcgccg ccgcgcgtcg tacacgcgga ggctcacgcc
     4681 gcgtctccgg aggaagtggc cgcctgggcc ggggcgtccg ccggcgcggc ttgctgcacc
     4741 gcggccgggg cgtcgccggc caccggcacg tggacgagca cgcccgccgc gcggaggctc
     4801 tcgagcgcgg ccattcgaag catgcgcgaa acggcgagac gctcctcggg aacggtcttc
     4861 gcctggagca tgatcgtgaa ctggcccgtc cccacgtcgt cgatgccgag gagcctcggg
     4921 ggttcgagga ggagcggctt cgcattcggg ctggccgcga accggacgag catcgcctcg
     4981 agcacctgca ccacgcgcgc cggatcctcg gtgagactga tcgggatccg gacgatcggg
     5041 gtggaccagc tcttcgttag attggtgacc accttgatct cgccgttcga gatgaaatgg
     5101 atgaagccct cgccgtccct gagctccgta tgcctcaacg tgagcgtctc gaccgtcccc
     5161 gacacgccca tggtccgcac gacgtcgccc acggcgaatt gattgtcgtg gatgatgaag
     5221 aatcccgcga tccaatcctt caccacgctc tgcgcgccga gccccgccgc aacgccgaat
     5281 cctcccgcgg ccgcgagagc cggggtgatg tcgagcccca gctcgcgcac gcacatgagg
     5341 atcccgatca cgatgatgat gccccgcgag aagccccgaa gcacctcgcc gatcgtgcgg
     5401 gcccgcttct cgcgcgcgag ctcggtcccc ttcgcctccg accggatcgc gcgctcgatg
     5461 cggccgatga ggaggtacac gagccggacc gtgaccagcg tggccaggat gatgatgccg
     5521 atccgcacca ggatctcgac cagcgtgaag gcctgctccg gcgaggccgt gtggcggacg
     5581 ggattcatga agctctccgg ggtcgagtca tcgctgcgtc agcgcgccgc gcggaccggc
     5641 gcggactcga cgatcgcctt gaccaggtcc tcgtagcgga gtcccgcggc ctgcgccgcc
     5701 ttggggacga ggctcagctc ggtgagcccg ggcaccgtgt tcacttcgag gcagtgggcc
     5761 acgccgtcct tcccgagtcg gaagtcgacg cgcgcgacgc cgctcgtgcg gagcgcgcgg
     5821 cacgcgcgga ggctgagcgc gaagagctcc tcggccacct cgggcgggag cgaggccggg
     5881 cattcgtagc ggctcgcgcc cgacgtgtac ttgtgctcgt agtcgtacca gccggccgtg
     5941 ggaatgatct cgaccacggg gagcgcgcgc tggccgatca caccgaccgt gagctcgcgt
     6001 ccgtcgatga actgctcgac caggacgagg cgaccgtagc ggcgcgcctc ctccatcgcg
     6061 ggcttccact ccgacgcgct cttgacgacg gtgatcccga cgctcgagcc ctcctcgttc
     6121 ggcttcacga tgagcggata gcccccgagc cgcgcgagct cctcggccgt gatctccgga
     6181 tgccacgccg cggccggatc ttcgggggcc caacgctcga cccactcggg ggtcgggatg
     6241 ccctcgacct tgaagatccg cttcgacatc accttgtcca tcgcgagcgc cgacgagagg
     6301 acgccggagc ccgtgtaggg aatgcccgcg agatcgagga gggcctggag cgtgccgttt
     6361 tcgcccgcgc cgccgtggag cgcgatgaag acgaggtcgg ccgtgcgcgg gatggagcgc
     6421 acgtagacgt cgagcccctg ctcgccccgc cgcgcgggca atccgcccgc ggcgtggagc
     6481 ggcgccgggc tcgcgccgag cgccatcgcg tggtccggat ccttgaggaa ctgtcccgtg
     6541 ccggtgtcga ggagcgccac gtcgtggccg agcgtctgga gcgcgcgagc cacgcccgcg
     6601 ccggtgcgaa gggagacttc ccgttccgag gtgttgccgc ccaggaggac gacgatcttc
     6661 atgctccctc cacgatcact cgcgcccttc cacgagcgcg accgcccacg cggcgatccc
     6721 ttcctcacgt cccagtgctc cgagtccttc gttggtcgtg gccttgaccg acacgcgtcc
     6781 ttccccgatt ccgagcgcta ggccgagcgc ttcgcgcatc gccgcgacgt gcggggcgat
     6841 gcgcggagct tccgcgacca gggaggcgtc cacgttcacg atcgtgtatc cgcgctcgcg
     6901 cagctgctcc gcgatcgtct cgaggagacg gaggctcgag gcgccgcgcc acctaggatc
     6961 cgagtcggga aagtgcgctc ccaggtcgcc caggccggcc gcgcccagga gcgcgtcgcc
     7021 gatcgcgtgc gcgagcacgt cgccgtcgga gtgcgcccgc aggcccttgt cgtgcgcgat
     7081 cgccacgccg cccagcgtga gcgggagtcc gcgctcgagc cggtgcacgt cgtatccgat
     7141 gccgacccgg agcgtggtcc cggacgtcac ggcgatccgc gacggaggag gaactcgaac
     7201 atcgcgagat cggacggctc ggtcagcttg aggttcatcg ggtctccggg gacgaccgcg
     7261 accttggccc cggacgcttc gagcagcgag gcgtcgtcgg tcgcgtcccg cccctcggcc
     7321 tcggcggacg cgtgcgcgcg gcgcagtata gcatcggcga agatctgggg cgtctggacg
     7381 ccgaagagcc gcgcgcgatc gagcgtccgc accacgcgcc ccgcgtccac ctcggccacc
     7441 gtgtcccgca aaggcacggc ggggatcgcg gctcccgtga gccgcgcggt ctcgagcacg
     7501 cgtgtgatca agcgctcctc gacgagcgcg cgggcgacgt cgtgcaccgc gacgtaggga
     7561 agcccgagcg tgagcgcccg aatccctcgt cctaccgact cgcgccgggt cgcgccgccg
     7621 ggcgcggcct cgcggcgaat gtccgaagac gccgggagcg cgctcaacgc cctccgcgcc
     7681 ggctcgagga gcgcttcggg aacgacggcg atcagctccc ccacggaggg atgcgcgaga
     7741 aagatcgagg ccacgcgctc gaggagcgtc ttccccgcta tttgtaggag aagcttcggc
     7801 ccgagccctc ccatccgcac tccgtcgccc gcgcccgcaa gcaccacggc gaggtcgcgg
     7861 cgagacgtga tgctcaccgg gcgtgatccg ggtcgagcac ccgtccgatc gcttcgtgga
     7921 ggctcgcggc tccgagcgca cccgtgacgc cctccggcgc ggagcgcttc gggacgatcg
     7981 cggcgcggaa tccgagccgc ctcgcctcgg cgagccttcg ctcgagctga ggcacgggac
     8041 gaagctcgcc gccgagcccc acctcgccga tcgcgacgag atcgggaggg agcggccggt
     8101 cgcggaagct cgacgcgagc gcgagcgcga gcccgagatc ggcggccggc tcctcgagcc
     8161 ggatcccccc cgcgacattg acgaacacgt ccgcccccgc gagcgagagc cccgcccgct
     8221 tctcgagcac cgcgatcacc acggcgagcc ggcgcgcgtc gatgccctgc gccacgcgct
     8281 gcgggttcgc gaagtgggtg ggagcgacga gcgcctgcac ctcgacgaga agcggacgcg
     8341 atccctcgat ggtcgcgacc acggccgagc ccgggaccga ttcgccccgg tctttgagca
     8401 gacgctcgga gggattctcg acttcggcga gaccgtctcc ctgcatcgtg aagacgccga
     8461 tctcgtgggt ggagccgaag cggttcttcg cggcgcccag gatccggtac tcgtggtgac
     8521 gctccccttc gagatagagg accgcgtcca ccatgtgctc gagcgtgcgc ggccctgcga
     8581 ccgagccgtc cttcgtgacg tggccgatca agagaacggg aaggttgcgc tccttcgcga
     8641 gcctcaagag cctgagtccg cactcccgca cctgggacac gctgcccggg gccgcgtcga
     8701 gcgagcccag gtagagggtc tggatggagt cgatgacgag gagcgacggg ttcttgcgcg
     8761 cgacctcctc gaggatccgc tccaggtcga tctccgactg catccagagc cggtcgagac
     8821 cgccgcgcaa ggcgaggcgc tcggcgcgga gcttcacctg gcgcggcgac tcctcccccg
     8881 agacgtagag caccgaccga tcggcgcgtc cgagcgcagc cgcgagctgg agggcgaggg
     8941 tggacttccc gatgccgggg tcgccgccga tcaggacgag cgagccgggc acgagcccgc
     9001 cgccgagcac gcggtccagc tcgccgatgc cgctcaccca gcgcggctcg gcgcccgagt
     9061 cgatctcgtc gagcctgagc ggagccgatg cgacgtcggg cgacgcgtag ttcgagcggc
     9121 gcgcgggggc ggcctcacgc cgccccgggc cttcgcgcgc gctgttccac gcgtcgcagt
     9181 gcgggcagcg cccgaaccac ttcgggctct cgttgccgca ctcggcgcag aagaagacgg
     9241 tcttcgcctc gcgcgctggg gtagccttcc tgggcgtcat ggggggtgag tctaggacga
     9301 agtggggtgt tcttagaaga cgctcagatt gatgtatttc ttcgggttct tctggatgtc
     9361 cttcacgagc tggttcgtgt tccggagcgt ctccttcagg tcgacgtaga gcgtggagtc
     9421 ggagatcgcc ttgccgagcg ctccctcccc cttgtcgagg cgcgtcacca cgcggtccag
     9481 cgagaccgcg gtggtcgaga gctggtccga gaggtgggag agcttccgcg acgcctcccc
     9541 gaagtccttg accgaggacg acacctggcc gccattcgcc gaggcgatgg cgcggaggtc
     9601 gtgcgcggcc gcgcggaagt ccacgatcgc ctgccggagg tcgccctggt tctggttcag
     9661 ggtctggttc agcccctcgc tcgccttctc gaagttcttc gacgcgcgct cgaccgtggc
     9721 cgggtcgatc gcgtcgagca tgagattgag gcgcgacgag agctcggtcg tctcgatgag
     9781 gaggtcgccc gcaccgctga tgacctcggg cacgcccgac tggaagcggc cggggatcgg
     9841 cttccgcccg tcgtaccgtc cgggcgcctg gccgggatcg agcgcgagga acttctctcc
     9901 catgaacccg atgttggcca cgcggagctg cgcgtcggga tggagcgtca cgcccttgtc
     9961 gacctcgaac tccacgcgca cgccctggtc ctcgagcacg atgttggtca ccgccccctt
    10021 gcgcacgccg cgcacggaga cgggatcgcc ctcaccgagg ttccccacct cctcgaagcg
    10081 ggcggtgtag tagctcttcc gcttgccgac ccggtactcc ttgatccagg cgatgccgat
    10141 gaagagcagc aggatcgaaa ggagtcccgc gactccgacc tgaatcgcgg cccgacgtga
    10201 cgttcggttc atccgtgatc ctttacgtgg ggaggagggg accgtccgag ctcccctcga
    10261 tgaattgacg caggatcggg tcccgtgtgg actggacctc ctccggcgtc ccgatcgcat
    10321 ggatgcgtcc ctcgtggagc atcgcgatgc ggtcggcaat atggtacgcg cttctcatgt
    10381 cgtgggtgac cgcgatcgag gtcatcccga gtctttcttg gagccggctg atgagccggt
    10441 tgatgacgtc cgccgtgatc gggtcgagcc cggtcgtcgg ctcgtcgtag accatgatct
    10501 tcggattcag cgcgagcgcc cgcgcgagcg cggcgcgctt cttcatgcct ccggacaggg
    10561 aggacgggcg ctgcccctcc gtccccgtga gtccgaccag cgcgagcttc tcggccacga
    10621 cctcctcgat ctggcgctcc gggagcggcg tgtgctcgcg cagcgcgaga cccacgttct
    10681 cccccaccag catggaatcg aacagggcgc ctccctggaa cagcatcccc atctgctcgc
    10741 gcaacgcgag gagctcccct tcgtccagct tcgcgacgtc gatgccgttc acccgcaccg
    10801 tcccccgatc cggttggagg aggcccacca cgtgcttgag caggaccgac ttcccggtgc
    10861 cgctccgccc gatcacgacc aggcactctc ccctcgaaac ggatagatcg acaccccgga
    10921 gcacaacttt cgtcccgagt cgcttccaga ccccctggaa ctcgatcgcg ttatcaggca
    10981 aagagcaccc ggaagaggaa ggaggcgagg aggtagtcca cgatcaggat gaggaggcag
    11041 gaggccacca ccgcccgggt ggtcgaatcc ccgaccgcct cggctccgcc ctcggtgcgg
    11101 agtccgtagt aacaccccat cgtcgcgatg attccaccga agaagaccga cttgaggagt
    11161 cccgagaaga cgtcctggat cttgaagtag agcttgagac cgctcgtgaa cgtgtggccg
    11221 ctcacgtcca gcgtgagcac cgccacgaga tatccgccga agatcgcgag gatgtcggcg
    11281 aagatcgtga gcatcggaag catcaggacg gaggcgacga gccgcggcac cacgaggtag
    11341 cgaacggggc tgatgccgag cgtctccatg gcgtcgatct gctcggtcac gcgcatcgag
    11401 ccgagctcgg ccgcgatcgc cgcgctcacg cgtccgccga ccacgagcgc tgtgagcacc
    11461 gggccgagct cgatcaccac ggatttcccg atgaccgtgc cgaggtagat catcggcacg
    11521 tagtcccgca tctggtaggc ggcctgcacg gcggcaaccg ctcccgtgaa gagcgccacg
    11581 atgacggtga gcggcatgga gccgagcccg atcgacacca tctgctcgaa gacgaggccg
    11641 atcttgcgga cgcccgcggg catctcgcgc agcgcgctcc cgacgaggag gaagatctct
    11701 cccgcgttct gcagccgcgc gaacaggaag cgctgcggcc acggagccgc ggccagggcg
    11761 gcgtcttcct gctcggcggt ctcggggtcg atcaccgggg ctcgggtcgg ggctccggaa
    11821 cgtcgcggag ggactcgaac cgggtgcact cgcggaggaa cgcgagatcc acgcttccgg
    11881 tcgggccgtt ccgctgcttc gcgatgatga tctccgcttt cccgtccatc ttggacgggt
    11941 cggtctcgta gacctcgggg cgatagacga agagcaccac gtcggcatcc tgctcgatgc
    12001 gcccgactcg cgcagatccg agagcatggg gcgcttgttg ccgccccgcg tctcgacggc
    12061 gcgcgagagc tgggacaggg cgagcaccgg cacctggagc tccttcgcga gcgccttgag
    12121 cgagcgcgag atgctcgaga tctcctgctg gcggttctcg gcgttgttgg ggccgcggat
    12181 gagctggagg tagtccacga cgatcatccc gagcggctgc tcggacacga ggcggcgcgc
    12241 cttggcgcgc atctcgagga tcgagatcgc cgcggtgtcg tcgatgtaga tcggcgcctc
    12301 gtggagctgc gcggccgcgg cctggagcgc cttgtactcg gccgcgctcg cgtaaccccg
    12361 gcgcaccttg tggatgtcca ccttggcgag cgagcagagg agccgttgca cgagcgactc
    12421 cttcgacatt tcgaggctga agaccgcgac ggtctggcgc tccttcccgc gcgcgcgcac
    12481 ggccacgtag tcggcgatgt tcatcgcgaa cgacgtcttc cccatcgacg ggcgtccggc
    12541 gatcaccacg aaatccgcct tctggaatcc ggccgtcatc tcgtcgaggt cgtagaagcc
    12601 cgaggggacg ccggtgacgt gcaccttccg ctgacgatac tcctcgatcg cggaataccc
    12661 ctggagcacg agcgagcgca tcggcaggaa gccggtgcgg aggcgctcgt cggagatcgc
    12721 gaagatctgc tgctccgcgc gatccagaag gtgcgctgga tcctcgcggc cgtcgaaggc
    12781 ctctcccacg atctcggttc cgacctcgat cagccggcgg aggatcgcct tctcgtggac
    12841 caccttgcag tagtgctcca cgtgcgcggg cgtcgcgatg aactgatcga gcgtggtgag
    12901 ataggtcacg ccccccacgg actcgagctc gcgccgcttc ttcagctcct cgaccagcgt
    12961 gaccatgtcg accggcgtcg agccgtcgaa gagatcgatg atcacgcgcc agatcttccg
    13021 gtgcgcgtcc ttgtagaagg cttcttcttg aagattctgg atcgcggtgg cgatcgcgtc
    13081 gcgcgagagg agcatcgcgc cgagcacgga gcgctcggcg tcgagcgcct ggggcgggat
    13141 gcgcgccgcg tcgaggccgg ccggagccat gtcgtcgatg cctctcacgc gggcgctcct
    13201 tcgcgcagca agtcgcggac gcgctggaga tccacccagg cgtccttctt gaggcccggg
    13261 ttccggagga gcgccgccgg atggtaggtc gcgacgagcg gcacgccctt gtactcgaac
    13321 atgcgcccgc ggagcgcgcc catcgacgct ttggtgccga ggagcccctg tgcggcggag
    13381 agcccgaggg cgacgatcaa gtccggccgc accaaggcga tctgctgctc gaggtagggg
    13441 aggcacttct ccacctcgga agggatcggc gtgcggttct cggggggacg gcacttgagc
    13501 acgttcgcga tgtagacgtc cgcgcgcttc atctggatcg cctcgatcat cttcgtgagg
    13561 agctgccccg cgcggccgac gaagggaact ccctggagat cctcatcgcg tcccggcgcc
    13621 tcgcccacga acatgagccg cgccttgtcc gtgccgtcgg agaagaccgt cctcgtgcgc
    13681 gtctcgcaga gcgggcagag acggcaggtg tcgaccagcg ccttcacctc ggcgagcgtc
    13741 ccggggagat cggagcgggg cgcgaggcgc acgggagccg gcatgggcgc ggaccagggc
    13801 gcggaaagcg gcggtgccgg gggctcgaag ccaggaagtg tgagaggggt cgcgccggcc
    13861 gtgggcgccg aggcgacggg agcctccggc gatcgcgacg cgctggcggc gcctcgtggc
    13921 gcagccggca tcggcgcctc gccgtcgatc ccgggcagct cgatcttgcg ccgtccgcgc
    13981 gcgagctcgc gccggaggag ggtcaccgct tcccccgcaa tcgccacgag ctcaccccgc
    14041 ccatcgtcca ccggcttccc ctcagtcttg attcagtgcc ttggccaccc ggagcaggat
    14101 cgctcgcgcg acctcgcgct tggggcgaag tcccagcgcc tcgacgcgtc cgtcggaatc
    14161 gatcagcgtc acgtcgttcg tgtccgagcc gaaggccgag cccggcttgc ccggattgtt
    14221 gatcgcgacg aggtcgagtc ccttgcgctg catcttaccc cgaccgcggt tgatctcatc
    14281 ctcggtttcg agcgcgaagc cgacgaggag ctttccgggg tgggccgcac ggacctcctt
    14341 caagatgtcg gtggtcggcg ccaggtcgag cttgagcccg ctcccctcgc gcgggagctt
    14401 cgatccgctc ggcttcgtgg gagcgaaatc gctcacggcc gcggccatga cgagcgcgtc
    14461 ggcatccttc accgcccgga gcacggcgtc cgccatggag gccgcggtcg gcgcgtcgat
    14521 ccgctcgacg ccggccgggg gctccgcggt gacgcgtgcg gtgatgagcc ggacccgcgc
    14581 tcccaggtcg cgcgccatag ccgcgatggc aaagcccatc ttgccgctcg agcggttggt
    14641 gatcacgcgc accggatcga tcggctcctc ggtcccgccg gcggtcacga cgatcgtgcg
    14701 cccatgaagc ggcagtccgt tcgcgcctga gccatcgcca cccgcgcccc tacccgcgcc
    14761 ggcaagcccc acgagcgacg cgcacgcggc gacgatggcc tcgagcgacg ccatccgtcc
    14821 catgccccac tcgccgtcgg cgagcgatcc ctcctcggga ccgaccatgc ggatcccgcg
    14881 cgcgcggagc acgtcgagat tcgcttggac ggccggatgc tcccacatgg cgacgttcat
    14941 ggcgggggcg aggaggacgg gcgagcgcgt ggcgaggagc gcggtcgaga ggagatcgtc
    15001 ggcaaggccg tgcgcgagct tgccgatcgc gttcgcggtg gcgggggcaa cgaggatcag
    15061 gtcggcctcg cgggccatcc cgatgtgcgc catgccgagc gcgctctcgg gcatcccttc
    15121 gaaccaggga cccgatccga gagcctgggt ctcgaagagc ccgtggagca cgggcccctt
    15181 ggtgaccgag cggagcgcgt ccttcgtgac gaagcgccgc gctcgcgtgg tcaggatgac
    15241 ggagacgtgc gccccttcct tcgcgagcgc ccggaccagc tcgggcgtct tgtacgcggc
    15301 gacgcctccc gtgatgccga ggaggacgcg cgcgccggcg agggaccgcg tctccgtcac
    15361 gtcacgctac tcttccgagt cggcgctcgg agcttcttcg ggctccgcgg gcgtctcttc
    15421 gtagtgatag agcacgttgc ccttcagggc ctcttccagc gcgacgttcg tcaccttgct
    15481 gcccaggttg aggcccgtct gacgtgagag ctcgttgatg cgacgggctt ccttcgccgc
    15541 cacgatgatg agctcgtact tgttcgggac gttgctcgag cgaagcgggg tcttggaatc
    15601 cacagatcct cctgactaga gttcggaccg accgccgtcg atccgccgcg aagcccggca
    15661 cgtctccgcc tcgatgatgg cgaggaccct ccgggtcgct tcttccaggt cgtcgttgac
    15721 gaccgcataa tcgtattcgc gcgcgatctc gatctcgcgc cgcgcgttct ccagccggag
    15781 cttgatgcgc tcctcgctgt ccgtgcctct cgcccggagc cgctcctcga gatccgcgag
    15841 cgacggcggc agcacgaaga tgaacacccc gtccgggtag atcctccgga ccgagcgccc
    15901 tccctggacg tcgatgtcga ggagcacgtt ccgcccggag cggagcaccg cctcggtctg
    15961 gctcttcagc gtgccgtaga gctcgccgtg cacttccgcg tgctcgacga aatccccggc
    16021 gtggacccgg cgctggaact cctcgcggcc gaggtagaag tagtcgcgcc cctccacctc
    16081 gtgcgcccgc cgggggcgcg tcgtggcgga caccgagtag accagatcgc gcgggtagcg
    16141 tccgaggagc accttcacga aggagctctt accggcgccg gacggccccg agatcaccac
    16201 gacgaagcgg cgatactccg agccgatcac gcccgcgtca ttcgatgttc tggacttgct
    16261 cgcggatccg ctcaagttcc tctttgacgt cgacgacctg ctcgatgatc ggaacgtcgg
    16321 ccgacttcga accgatcgtg ttgatctccc gattcatctc ctggaggagg aagccgagct
    16381 tccgccccgg cgtggcctcc tcgttcagca gcttcctgaa gtggtcgcag tgcgcctcga
    16441 gacgcacgca ctcctcggtc gcgtcgaggc gatccgccat catggcgacc tcctgcgcga
    16501 gacgctcctc cgggagctcg ttaccgtcga ggagaacgcg gatgcgatct tgaagcttct
    16561 ccgcggtctc cttgaccttc tggggtgcgc gcaggcgaat ctgcgccacg cgatcccgga
    16621 ttccctcgac ccgcgcctcg agatccgtcc ggagggccgc cccctccgtc tccttcatgc
    16681 ggatcatgtc ctcggtggcc gcggtcacga cctcgcgcag gattccgaga tagaactcgg
    16741 gatcgcgccg catctcgctc cacacgaaga gattggggag cgaggcgaat gtgtccacgt
    16801 ccacgctgcc cgaaaggctg tacttcctct ggagcgcctg gaggagctcc atgtaccgat
    16861 cggccgcccg ctcgtcgagg gtcagcgtgc ccgtgggctc gcgcccgtcg tctccgccct
    16921 cctcgcccca ggtcacgacg aggttgatct tcccccgcac cgaccgctcc tgcacgatct
    16981 tgcgagcctc gtgctcgtag atgccgaggg tccgcgggag acgtgcggag agctcgcaga
    17041 atcggtggtt caccgaccgt acttcggccg cgaggcgaag gccatccaca acgacctcgc
    17101 cccggccata gcctgtcatg cttcggatca tgctgagtct tcgacgctcc ccggggtagt
    17161 tgggtactgt aacccgtggc gcggcagcgc gtcatcaggc agattcacgt tgcgcgacac
    17221 gccgcccgct ccctaacgtc cggcccatgg cccctcgccc gcaagctcaa cgatcccaag
    17281 ggatggcggc cggagggaat cgcgcgagcg gcccggcggc cggcgcggct cgcgcgaggg
    17341 ccggcgctcc acgctggcga gcgtgggttc tcggggcggt cgtcgccctc gccgcgctcc
    17401 tcccgcacgc ccggtcgatc ggctacgact tcgtctggga cgaccgctac ctggtcggtg
    17461 agcacctcga catccacgga ccggcggacc tcgcccgcct ctggagcacg ccgttcgacg
    17521 tctacctcca gacgcagacc tcgcaacaca cctacttccg cccggcgacc gtctactcgc
    17581 tcgcgctcga tcgcgc
//
LOCUS       DASTKC010000222         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1608383, whole genome shotgun sequence.
ACCESSION   DASTKC010000222 DASTKC010000000
VERSION     DASTKC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608383"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFR25_09350"
     CDS             complement(<1..513)
                     /locus_tag="VFR25_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET9941295.1"
                     /translation="MSLINYSSREINCKIVYYGPGLCGKTTNLQHIYSRVPQETRGKM
                     ISLATEMDRTLFFDFLPLELGQIRGFRTRFHLYTVPGQVYYNASRKLILKGVDGIVFV
                     ADSQIDRFDANVESLVNMHDNLSEHGIATDKIPVVMQYNKRDLPRVVSVGDLERELNP
                     QGVPFYEAVAL"
     gene            complement(529..1032)
                     /locus_tag="VFR25_09355"
     CDS             complement(529..1032)
                     /locus_tag="VFR25_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9941296.1"
                     /translation="MVKGNWALFEEDFWSINAILQTLMRSASARSVMLIDKTGQLINS
                     IGEPPGFDVTSFSSLAAADFAANAQLAEMVGEKDFATLVHQGQNDSLYLSMIANRVIL
                     VVLFDRKTSLGLVRLKAKRAGDELLEVLNKLFDKLQYRNEEAIATPLGAEFAAEAESE
                     IDSLFQD"
     gene            complement(1266..1865)
                     /gene="recR"
                     /locus_tag="VFR25_09360"
     CDS             complement(1266..1865)
                     /gene="recR"
                     /locus_tag="VFR25_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET9941297.1"
                     /translation="MQYSSALLETLIAELTRLPGLGRKSAQRIAFHLLRSPETDSQRL
                     ASAILELKANVHDCSICGNVTETDPCALCADPRRDPSVLCVVEQPMDVLALERTGEFH
                     GRYHVLKGALSPVDGIGPDQLRLKELIERARQGVAEVIVATNPTAQGEATALYIARLL
                     HNEPSVKVTRIARGVPMGSDLEFSDQITLARALTGRKEL"
     gene            complement(1882..2193)
                     /locus_tag="VFR25_09365"
     CDS             complement(1882..2193)
                     /locus_tag="VFR25_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET9941298.1"
                     /translation="MNIQKMLKEAQALQAKMSKAQEELATKEETGSSGGGKVKITMNG
                     SQEVTAVAIDPAVVNPDEADLLEDLVLTAFKDARSKVTKLIEEEMGKVTGGTGLPPGL
                     F"
     gene            complement(2190..>2603)
                     /locus_tag="VFR25_09370"
     CDS             complement(2190..>2603)
                     /locus_tag="VFR25_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941299.1"
                     /translation="DEPSLADSALAARWREIVDRVKERKLLLGTCLEEGLFLGVQGSS
                     VRVALTAEHSFHRAMLEMKENKDILNQEFERTYGPGATLLCVGSDQAVTGAGIRPVPR
                     EATQAAPAPSGLVQRIVELFDGEILGQGPEGSGAT"
BASE COUNT          421 a          842 c          871 g          469 t
ORIGIN      
        1 caaggccacc gcctcgtaga acggcacccc ctgcgggttc agctcccgct ccaggtcccc
       61 caccgagacc acgcgcggga ggtcacgctt gttgtactgc atcacgaccg ggatcttgtc
      121 ggtcgcgatc ccgtgctccg acaggttgtc gtgcatgttg acgaggctct cgacgttcgc
      181 gtcgaagcgg tcgatctgcg agtcggcgac gaacacgatg ccgtcgaccc ccttcaagat
      241 cagcttccgg ctcgcgttgt aatagacctg ccccggcacc gtgtagagat ggaagcgcgt
      301 gcggaagccc cggatctgac ccagctcgag cggaaggaaa tcgaagaaga gcgtgcggtc
      361 catctcggtc gccagggaga tcatcttccc ccgggtttcc tggggaacgc gcgagtagat
      421 atgttggaga tttgtggtct taccgcaaag ccccgggccg taatagacga tcttgcagtt
      481 gatctcccgg gacgagtagt tgatcagcga cacggagcgt gcatacccct agtcctggaa
      541 gagactgtcg atctcgctct ccgcctcggc cgcgaactcg gcacctagag gcgtcgcaat
      601 ggcctcctcg tttcggtact ggagcttgtc gaagagcttg ttcagaacct cgagcagctc
      661 gtccccggcg cgcttggcct tcaagcggac cagtccgagc gacgtcttcc ggtcgaagag
      721 cacgaccagg atgacccggt tcgcgatcat ggagaggtag aggctgtcgt tctgcccttg
      781 atggaccagg gtggcgaagt ccttctcccc gaccatctcg gcgagctggg cgttggccgc
      841 gaagtccgcg gcggcgaggg atgaaaacga ggtgacgtcg aagccggggg gttcgccgat
      901 cgaattgatg agctgccccg tcttgtcgat cagcatgacg ctacgcgcgc tggcgctgcg
      961 catcagcgtc tggaggatcg cattgatcga ccagaaatct tcttcgaaga gggcccaatt
     1021 ccctttgacc atgtcctggc tccctggaca cctggccttg cggtgcgctg gaaacagcga
     1081 cgcccgcggc tcaaggggtg tccttccctt gccccgcgca cgcgccggtg gcgagaccga
     1141 ttccccccac accatagctg ctctgcgcgg attccctccc gcgcatcgcc ccgtcaaagg
     1201 aattggcgga aacctaccga gcgggcgcgg agagggtcaa gcggaaagtg ccccaaaccg
     1261 gaggcttaga gttccttccg gccggtcagg gcccgggcca gggtgatttg atcggagaat
     1321 tcgaggtcgg accccatcgg cacgccgcgc gcgatgcggg tcaccttcac cgagggctcg
     1381 ttgtggagga gccgcgcgat gtagagcgcg gtcgcctccc cctgcgccgt gggattggtc
     1441 gccacgatca cctcggcgac gccttggcgc gcccgctcga tgagctcttt gagccggagc
     1501 tggtcggggc cgatcccgtc gaccggagag agcgccccct tgaggacgtg atagcggcca
     1561 tggaactcgc ccgtccgctc gagggcgagg acgtccatcg gctgctcgac cacgcagagg
     1621 accgacggat cgcggcgcgg atcggcgcag agggcgcacg ggtcggtctc ggtcacgttc
     1681 ccgcagatcg aacagtcgtg cacgttggcc ttgagctcga ggatggccga ggcgagccgc
     1741 tgcgagtccg tctcgggcga gcggaggagg tggaacgcga tccgctgggc ggattttcgg
     1801 ccgaggccgg gaaggcgggt cagctcggcg atgagcgttt cgaggagggc gctggagtac
     1861 tgcatgctgg gggagtgtgc cctagaagag accgggcggg aggcccgtgc cgcccgtcac
     1921 cttccccatc tcctcttcga tcagcttcgt caccttggag cgcgcgtcct tgaacgcggt
     1981 caggaccagg tcctcgagga ggtcggcctc gtccgggttc acgacggcgg gatcgatggc
     2041 gacggcggtg acctcctgcg agccgttcat cgtgatcttg accttaccgc cgccggagga
     2101 gcccgtctcc tccttggtcg ccagctcttc ctgcgccttg gacatcttgg cctggagggc
     2161 ctgcgcctcc ttcagcatct tctggatgtt catgtagcgc cgctcccttc gggcccttgg
     2221 cccaggatct cgccgtcgaa gagctccacg atgcgctgga cgagcccgct cggagcgggc
     2281 gccgcctggg tcgcttcacg cggcacggga cggatccccg cacccgtcac ggcctgatcg
     2341 ctgcccacgc agagaagcgt agcacccggc ccgtaggttc gctcgaactc ctggttcagg
     2401 atgtccttgt tctccttcat ctcgagcatc gcgcggtgga aggagtgctc cgcggtgagc
     2461 gcgacgcgga cgctcgagcc ctgcacgccg aggaacaatc cctcctcgag gcaggtgccg
     2521 aggaggagct tccgctcctt gacgcggtcg acgatctcgc gccagcgggc cgcgagcgcg
     2581 gagtcggcga gcgacggctc gtc
//
LOCUS       DASTKC010000223        10286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1371904, whole genome shotgun sequence.
ACCESSION   DASTKC010000223 DASTKC010000000
VERSION     DASTKC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371904"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFR25_09375"
     CDS             <1..955
                     /locus_tag="VFR25_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941300.1"
                     /translation="LRSPDVDDAEERSLAIYRGLQKSETEMRSVVYEVNRLLGTQYSL
                     ERRARDLDEHLLWEFLDPLDRFVDDLFEAVSRRKGEPTLLTVQTGGVGFEPEIGSTLI
                     PILSRLLETGDVPPATNGVPEVRVAAARESLEAMIAISGFTRLDPEALTMLERALEGL
                     AGFVEVSGEDIISLRIQFPMARALRSFLIVEAAGHKLALPWSAVDRVDANGEVPAEDP
                     DMPALALHTLFATATARRSAPGEPEEARPVAILRSGGRITRVTFDRIVWRESARWAPL
                     PAQLRTTDEVLGGIVAPDGAITLVLNPATIADRARSREVAA"
     gene            952..2577
                     /locus_tag="VFR25_09380"
     CDS             952..2577
                     /locus_tag="VFR25_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9941301.1"
                     /translation="MKALKIERAGAERFHFVLEGEVDPAGARSVDRLLFTCQARGARD
                     VRLDFSGVTSICTLGTAVLVRQGKVYQDTERRIQVIGLSPEIRRALMDVEAIVYETNA
                     GAAPDAMPAMPDAAAAPADAPAAAAPAAVPDVIPPREDASDEIALLQSKLKRKLVEFR
                     NLFEITRALNLAVDLDELLNLFSLSIMGQFGVERVAVFLVEPRRGGLFAPRQVRGFPS
                     DHFRDFTMPADVLEHRGADEEFLSLGDLGSAEAGIEALRASGLEWLVPLRVRRNLEGL
                     VFLGGRGAGRGFHEDERDLLTTLCNQGAVAIANAQQRRAQEDRNLGLVRGMMSLIESR
                     DEYARGTTERVVRYVTAVSKLLNVPKPALKSLVYGAALRDIGMIAVSHLILKNPAHLS
                     DDEWALIKQHPERGARILEEMDFPSEVIDVVRHHHERWGGEGYPDGLRGNEIPLGARI
                     VSLVDSYVAMTGERPYRRALPFDKARQVVAENWGTPFDPSVVEMFLQVLDNLERRSRQ
                     RPNASSSNASPPEGADAGLEPGGPGAIPLESHP"
     gene            2574..4208
                     /locus_tag="VFR25_09385"
     CDS             2574..4208
                     /locus_tag="VFR25_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9941302.1"
                     /translation="MSLESIRPAAEPGAAKTKRSILVIDDDRRVLELLQISLTQNGFK
                     VAVAATGEEGLDQMRKDAPDLVILDLRLPRKTGFEVCAAIKSSKDTQHIPIIMVSASA
                     EVDSRLQGLMHGADDYLTKPFSPKELLIKVRRIFERLDRAEHLATKNRELESEVARNR
                     EDLVARNKELRFQVYSLETLMGLTHQLNSSLDLDDLLNTLILSVVGQLRVNSACLFLT
                     DDRDEPKVLQVSTFKGLKGDQMREIHFPYSSPFIGALLPPDGEEGRPVRLSDLEDDPA
                     LAAGVGPLFAAGLAVVSPVVMKQRLTAVLAVGEKVSGQEFGTVDLEMLRALSESAGIA
                     IENARLFKDLQESYVSTVRLLVARIEEKDPYTHGHTERVAKYSVALARELGFPVEEVQ
                     RIQFGAYLHDIGKVHTQDEVLHKPGALTDEEWRMVKMHPVRGAEMVKGVKFLERVVDM
                     IRHHHERVDGRGYPDGLKGDEISTAAKIVNVADAFDAMTTDRPYRAGMTFDQAIAQMQ
                     EKAGTQFAAEIVEVLVRAVRGGRFEIGKSGATVATS"
     gene            complement(4260..5489)
                     /locus_tag="VFR25_09390"
     CDS             complement(4260..5489)
                     /locus_tag="VFR25_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941303.1"
                     /translation="MIHLTVQQLSSYLDGQLVGSASGNVQSHLAACRDCSTRFALLER
                     IETILSDSLSIDPGEEFFRELEAEVGSRIRERGQRDKDLSTPAVSSVRLLEHPARAAS
                     ARGDSRAANKGPDVPAANTRTDSRIEDTANRVTPARRVEPRAPRPHRPLRTKRSGSSG
                     AWLAFAALAVVAGSVGVVVSRTSFVSAWIAPHLGLGPEQRSLASRALSPPATPETTAT
                     ETPAVEPPGDPTPSDAGNAGHEWNPWEPPPSHSSTADDEMTPDSNREPDADDQAASPE
                     PSTMRGDEVIATPDSSRKSRATGSDPFRALGTESLGPVRAAQQLSDMANADPTAARFE
                     VAAEGWERALDTMGGVPEQVSVRQLLADARYRAWQAEPTRAHAEAAKSAIKTYLLFAP
                     SGWEREQAKTRLSKLGG"
     gene            complement(5486..6133)
                     /locus_tag="VFR25_09395"
     CDS             complement(5486..6133)
                     /locus_tag="VFR25_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9941304.1"
                     /translation="MHGVSLVYLEAGEEIERIAAAQSGDRHAYLALVRQYARPLYRIA
                     YAMCGDADAADLLARDALICGWGELREIPESLRFFPWILGIMRRIHETPSAVANSPKA
                     TETRPMVAIDAHGAELSPSDGGSQTTEASPAQAGPAPAGDGTPGDARLRARTLASFAE
                     LRPDQRMTLSLRILEQLPYEQMAALLDLSLGVATLRLSQARGHILSRATGLGGDA"
     gene            7321..7551
                     /locus_tag="VFR25_09400"
     CDS             7321..7551
                     /locus_tag="VFR25_09400"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET9941305.1"
                     /translation="MTDKEPNKVTAWRQVGLLTSIPFILALAPIVGYFLGKFLDQKFR
                     TQPWLSFILLVLGFVAGVRETIHIVKLSQSED"
     gene            7548..9029
                     /gene="atpB"
                     /locus_tag="VFR25_09405"
     CDS             7548..9029
                     /gene="atpB"
                     /locus_tag="VFR25_09405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9941306.1"
                     /translation="MTPLIRNENAATNPLARAIWMSWLAAFALAGLLLPRTPGIALAI
                     VAGQSLMAINFLFLDKLVKLFTHAMLPGTGNRQIPGRFFGYASLKILLVYGGAFALIR
                     SGAVPFLPLVGGLSLPLLVLFLQSLMCAAGYAASPFSRRAPKTAATFGNAAKPAAFFF
                     ILALLAVPALGHASDDGAKAEPVVVAQHDPAQPTTPANPDMATQSDAAATAHGGAAGH
                     GEAASGEHAEGGEAHLPNWVVILNDYFPNNPVVHWMHENEYLVFAWLAGLVFFVVVWI
                     AMRKPTLVPGTLQNGIEWIVESMEDVCGGMLGKHLPKYFPFIMAIFFYILTMNLIGII
                     PGFKSSTSTLDVTLALALITFLYVQINGIINLGPLGYLDHLAGSPRDIVGFAMLPVMI
                     PVHILGELVKPVSLSCRLFGNIFGEDTLIVVFVGVAALALKASLTLLAVPPMAGITAL
                     FMLLQTLTAIVQALIFSLLTTVYLYMMLPHEEHAHGEAGHEHA"
     gene            9054..9287
                     /gene="atpE"
                     /locus_tag="VFR25_09410"
     CDS             9054..9287
                     /gene="atpE"
                     /locus_tag="VFR25_09410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET9941307.1"
                     /translation="MNFAATLGLALPIGIGLAAIGSGIGLGFMGRGAMEAMGRQPEVL
                     PKIQVAMIIGFGFIEALTIYAFVTMFFLQGKIQ"
     gene            9337..9828
                     /gene="atpF"
                     /locus_tag="VFR25_09415"
     CDS             9337..9828
                     /gene="atpF"
                     /locus_tag="VFR25_09415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET9941308.1"
                     /translation="MSQFVHWNQLLAHALSFVVFYFLVRMAFAAIIYPPMKERRDKIK
                     AEFERIESEKAAAERLRQEYEGHIKRIEAESRERIQNAVSEGQKVAAEIREIARKEAQ
                     DTIQRAKEEIAREHDKAAAKLKGEVVDLAMEIAGKVVREELTADKHRRLVDGFLTELE
                     QTR"
     gene            9825..>10286
                     /gene="atpH"
                     /locus_tag="VFR25_09420"
     CDS             9825..>10286
                     /gene="atpH"
                     /locus_tag="VFR25_09420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET9941309.1"
                     /translation="MISVTLARRYARALLDLAQRQNEVPRTEAELKTVGELFRKDPRA
                     RRFFETPNISREEKTRFLEERLMPRVCRPLYGLFHVLLRRRRLDHLVVIAEEFEKLAE
                     KAQGITHATVRTAAPITDAQADRLTQVLVRRMGGTIQLTREVDPSLLGGAAV"
BASE COUNT         1669 a         3465 c         3493 g         1659 t
ORIGIN      
        1 gcttcgctcc cccgacgtgg acgacgcgga ggagcgctcg ctcgcgatct atcgcggact
       61 ccagaagtcg gaaacggaga tgcgctcggt cgtctatgag gtgaaccggc tcctcggcac
      121 gcagtactcg ctcgagcgcc gcgcgcgcga cctggacgag cacctcctct gggagttcct
      181 cgatccgctc gaccggttcg tggacgatct cttcgaagcg gtttcgcgaa ggaagggaga
      241 gccgaccctc ctcacggtgc agaccggcgg agtgggtttc gagcccgaga tcggctccac
      301 gctgatcccc atcctgtcgc gcctcctcga gacgggagac gtgccgcccg ccacgaacgg
      361 cgttccggag gttcgggtgg ccgccgcgcg ggagagtctc gaagcgatga tcgcgatctc
      421 gggcttcacc cgactcgatc ccgaagcgct cacgatgctg gagcgcgcac tcgagggcct
      481 cgcgggattc gtcgaggtca gcggcgagga catcatctcg cttcgcattc agttcccgat
      541 ggcgcgcgcg cttcgaagct tcctcatcgt ggaggccgcg gggcacaagc tcgcccttcc
      601 ctggagcgct gtggatcgcg tggacgcgaa cggcgaagtg cccgccgaag accctgacat
      661 gccagcgctc gcgcttcaca cgctctttgc caccgccacg gcacgccgga gcgcgccggg
      721 agagccggag gaggcgcgtc ccgtggccat ccttcgctcg ggggggcgca tcacgcgcgt
      781 cacgttcgac cgcatcgtct ggagggagag cgcgcgatgg gcgccgcttc ccgcgcagct
      841 gcggaccacc gatgaggtgc ttggcgggat cgtggcgccg gatggcgcca tcacgctggt
      901 gctgaaccct gccacgatcg ccgatcgggc gcggagccgg gaggtggcgg cgtgaaggcg
      961 ctcaagatcg aacgagccgg tgcggagcgc ttccacttcg tcctcgaggg agaggtcgat
     1021 cccgcaggag cgcgctcggt cgaccgcctt ctcttcacat gccaggcgcg gggggcgcgc
     1081 gacgtgcggc tcgacttcag cggcgtgacc tcgatctgca ccctcggaac ggcggtgctc
     1141 gtgcggcagg ggaaggtcta ccaggacacg gagcggcgca tccaagtgat cggtctctcc
     1201 ccagagatcc gccgcgcgct catggacgtg gaggcgatcg tctatgagac gaacgccggg
     1261 gctgcgccgg acgcgatgcc ggcgatgccg gatgctgccg cggcgccggc ggacgccccg
     1321 gccgccgccg ccccggctgc cgtgcccgac gtgatcccgc cccgcgagga cgcttcggac
     1381 gagatcgcgc tcctccaatc gaagctcaag cggaagctcg tcgagttccg gaacctcttc
     1441 gagatcacac gcgcgctcaa cctcgcggtc gatctcgacg agctgctcaa tctcttcagc
     1501 ctgagcatca tgggtcagtt cggcgtcgag cgggttgccg tcttcctcgt cgagccgcgc
     1561 cgcggcggac tcttcgcgcc acgtcaggtg cggggcttcc cgtcggatca cttccgcgac
     1621 ttcacgatgc cggcggacgt cctcgagcac cgcggcgccg acgaggagtt cctgagcctc
     1681 ggagacctcg gatccgccga ggccggcatc gaggcgcttc gcgcctccgg cctcgagtgg
     1741 ctcgtgccgc ttcgcgtgcg ccgaaacctc gaggggcttg tgttcctcgg aggccgcgga
     1801 gcaggccgtg ggttccacga ggacgagcgc gacctcctga cgacgctctg caaccagggg
     1861 gcggtcgcga tcgcgaacgc ccagcagcgc cgcgcccagg aggaccggaa cctcggtctc
     1921 gtgcgcggca tgatgtccct gatcgagagt cgcgacgagt acgcgcgcgg gacgaccgag
     1981 cgcgtggtgc gctacgtgac cgccgtgtcg aagctcctca atgtcccgaa gcccgcgctc
     2041 aaatcgctcg tttacggcgc cgcgctccgg gacattggca tgatcgctgt gagccatctc
     2101 atcttgaaga atcccgcgca tctctcggac gacgagtggg ccctgatcaa gcagcatccc
     2161 gagcgcgggg cgcggatact cgaggagatg gatttccctt cggaagtgat cgacgtcgtg
     2221 cggcaccacc acgaacgctg gggcggagaa ggctatcccg acggtctgcg cgggaacgag
     2281 atcccgctcg gggcgcgcat cgtctcgctc gtcgactcct atgtcgcgat gacgggcgag
     2341 cgcccgtacc ggcgcgcgct tccgttcgac aaggcgcgcc aggtggtggc cgagaactgg
     2401 ggcacgccct tcgatccgag cgtcgtggag atgttcctcc aagttctgga caatctcgag
     2461 cggcgctcgc gtcagcgacc gaacgcctcc tcgtcgaatg cttcgccccc tgaaggcgcg
     2521 gacgcgggct tggagcccgg cggtcccggc gccattcccc tggagagcca cccgtgagcc
     2581 tcgagagcat ccgtccggcc gccgagcctg gagctgcgaa gacgaagcga agcatcctcg
     2641 tgatcgacga cgatcgccgc gtgctcgagc tgcttcagat ctcgctcacg cagaacggct
     2701 tcaaagtcgc ggtcgcggcc acgggggaag aggggctcga ccagatgcgg aaggatgcac
     2761 ccgatctcgt gatcctcgat ctgcgcctgc cgcggaagac gggtttcgag gtctgcgccg
     2821 cgatcaagag cagcaaggat acgcagcaca tcccgatcat catggtctcg gcgagcgccg
     2881 aggtcgattc gaggctccag ggcctcatgc atggcgcgga cgactacctg accaagccct
     2941 tctctccgaa ggagctcctg atcaaggtgc ggcgcatctt cgagcgtctc gaccgggcgg
     3001 agcacctcgc gaccaagaat cgcgagctcg agagcgaggt cgctcgaaac cgcgaggacc
     3061 tcgtcgcgcg gaacaaggag ctgcggttcc aggtctacag tctcgagacc ttgatggggc
     3121 tcacgcacca gctcaactcc tcgctcgatc tggacgacct cttgaacacg ctgatcctga
     3181 gcgtcgtggg acagcttcgc gtgaactcgg cctgcctctt cctcacggac gaccgcgacg
     3241 agccgaaggt cctccaggtc tccacgttca agggactcaa gggggatcag atgcgggaga
     3301 tccacttccc ctactcgagc ccgttcattg gcgcgctcct gccgccggac ggagaagagg
     3361 gccgccccgt gcgcctctcg gatctcgaag acgatcccgc gctcgccgcg ggcgtcgggc
     3421 cgttgttcgc ggcggggctc gccgtggtgt cccccgtggt gatgaagcag cgcctcacgg
     3481 ccgtgctcgc ggtgggcgag aaggtgagcg gccaggagtt cgggactgtg gatctcgaga
     3541 tgctccgcgc gctctccgaa tcggccggca tcgcgatcga gaatgcccgc ctcttcaagg
     3601 accttcagga gtcctacgtc tcgacggtgc gcctgctcgt cgcgcgaatc gaagagaagg
     3661 atccctacac gcacgggcac acggagcgcg tggcgaagta ttcggtcgcg ctcgcgcgcg
     3721 agctcggctt cccggtcgag gaagtgcagc gcatccagtt cggcgcttat ctccacgaca
     3781 tcggcaaggt gcacacgcag gacgaggtgc tccacaagcc gggcgccctc accgacgagg
     3841 agtggcggat ggtgaagatg catcccgtcc gaggcgcgga gatggtcaaa ggggtgaagt
     3901 tcttggagcg cgtcgtggac atgatccgcc atcaccacga gcgcgtcgac ggtcgcggct
     3961 atccggacgg cctcaaggga gacgagatct cgaccgcggc gaagatcgtc aacgtggcgg
     4021 acgcgttcga cgcgatgacc acggaccgtc cgtaccgggc gggcatgacg ttcgaccagg
     4081 ccattgcgca gatgcaggag aaggccggga cgcagttcgc ggcggagatc gtcgaagtgc
     4141 tcgtgagggc agtgcgtggg ggccggttcg agatcggcaa gagcggagcc acggtcgcga
     4201 cctcgtagag aagtcgttgg cggcaggtag aatcgactcc cggcagcagc tccccggaat
     4261 cagcccccca gcttcgacag gcgagtcttc gcctgctccc gctcccagcc ggacggcgcg
     4321 aacagcaggt aggtcttgat cgcggacttc gctgcttcgg cgtgtgcccg cgtcggctcg
     4381 gcctgccacg cgcggtagcg cgcatccgcc aggagctggc gcaccgaaac ctgctcggga
     4441 actccgccca tcgtatcgag cgcccgctcc catccctcgg ccgcgacctc gaaccgcgcc
     4501 gcggtgggat ccgcgttcgc catgtcgctc agctgctgcg ccgcgcgcac cggtccgagg
     4561 ctctccgtgc cgagcgcgcg gaagggatcg gatcccgtcg cccgcgactt gcgggacgaa
     4621 tccggagtgg cgatgacttc gtctccgcgc atcgtcgagg gctccggcga tgcggcttgg
     4681 tcgtcggcgt ccggctccct gttggaatcc ggcgtcatct cgtcgtcggc cgtggaggag
     4741 tgcgagggcg gcggctccca tggattccat tcgtgccccg cattccccgc atccgacggc
     4801 gtcggatcgc cgggcggctc cacggccggg gtctcggtcg cggtcgtctc gggagtcgcg
     4861 ggcggcgaga gggcgcgcga cgcgaggctc cgctgctcgg gaccgaggcc cagatggggc
     4921 gcgatccagg cggagacgaa cgaggtgcgc gacaccacca cccccacgct cccggcgacg
     4981 acggcgagcg ccgcgaacgc gagccacgcc cccgacgatc cggagcgctt cgtccttaag
     5041 ggacggtgag gtctcggagc tcgaggctcg acgcggcgcg ccggcgtgac gcggttcgcg
     5101 gtgtcctcga tgcgcgagtc cgtgcgggtg ttcgccgcgg gaacgtccgg gcccttgttc
     5161 gcggcgcgag agtccccgcg cgcgctcgcg gcccgggccg gatgttccag gagccgtacg
     5221 gaggagaccg ccggtgtcga gagatccttg tcgcgctgcc cgcgctcgcg gatgcgcgag
     5281 cccacttccg cctccagctc gcggaagaat tcctctcctg gatcgatcga gagcgagtcg
     5341 gagaggatgg tttcgatccg ctcgagaagt gcgaagcgcg tcgagcagtc gcggcaggcc
     5401 gcgagatggc tctgaacgtt gcccgaggcg gatccgacga gctggccgtc gagataggac
     5461 gagagctgct ggacggtgag atggatcacg cgtcccctcc gagaccggtc gcacgcgaga
     5521 ggatatggcc gcgcgcctgg gacagccgga gcgtcgccac acccagggag agatcgagga
     5581 gcgccgccat ctgctcgtag gggagctgct cgaggatgcg cagcgaaagc gtcatgcgct
     5641 gatccggccg cagctccgcg aacgacgcga gcgtgcgggc gcgaagacga gcgtcacccg
     5701 gcgtcccatc accggccggc gcggggccgg cctgcgccgg cgaggcttca gtcgtctgcg
     5761 agccgccgtc cgacggcgag agctccgctc cgtgcgcgtc gatggcgacc atgggccgtg
     5821 tctccgtcgc cttcggtgag ttggcgacag ccgacggcgt ctcgtggatc cgccgcatga
     5881 ttccgagaat ccacggaaag aaacggagcg attccgggat ctcccggagc tcgccccatc
     5941 cgcagatcag ggcgtcgcgc gcgagcagat ccgcggcgtc cgcatcgccg cacatcgcgt
     6001 acgcgatgcg atagagcgga cgggcgtact ggcggacgag cgcgagatag gcatggcggt
     6061 cgcccgactg cgcggccgcg atccgctcga tctcttcgcc tgcttccaga tagacgagag
     6121 aaacaccatg catgcgtcga ccggcctcgc gtttgagagg gaaggaagga cgaccaattt
     6181 atcggcagtt cgggggtgtt ctggagggcg ggtgaaggtg ggtgcggccg gggcgggggg
     6241 aacggtgcgc tcccaacagg ctccggggcc agttggatgg acggcgcacc tgcgtttcta
     6301 ggtcgagact cggccggcgc cagtaggccg gtgccaccct tgtgggtgcc gtccggtctc
     6361 acgcagtcct tcgcaagctc aagtccacca tgacctacgg tcgctgagtg cctccggttg
     6421 actcgtccta cgactggtcg gtgggagcat tcgcgcttcg tcaactggcc ccggggccag
     6481 ttgcaatggt ggggacctca ccggaccgat gaccccaccg caagcggcca tcgagccccg
     6541 gggccggccg cccccccgcg ttgacggcgc gcaaccgtcc gttgatgtcg cgctaggtcg
     6601 ctcatcgcca gcagcttggg ccgcagccag agcgaaacca cggtccggag cgtcgaccag
     6661 cggttgcggc ccttcgatcg cacggcagag tgatgtggga cccaagtcgt tgcatggatg
     6721 aaagatacac ggagcgtcga tcatcggcac gtgcgttgcg atagcgcagt cacgtcggcg
     6781 gcccggcgct ggaaggaccc ctcaccggac ccggtaggcc cgcagctggg ccgcacgact
     6841 caatcgccct gtgagccggg ggcaccgaag cacgggagca ccgaagcacg gagcaccgaa
     6901 gcgaacgaag cacacccata tagatgtgaa ggcggggcgt cctctgtttg ggggagaaca
     6961 ctgcggagtc ggggccgcgg tgtggtgaag ccgaggaggc gaagaggcgc cccgccatga
     7021 gcttggggcc cagtccgcag ccgccgcagg cgccatcgag cgcccggagg gcagcgtcga
     7081 cgggcccctt cgttcgtcag ggcccgcacc ctgccaccgc cgcatccacg atcccaatga
     7141 ccccgcgatc ccacccgcac cccgcgatcc cacgcccata cccccgccga ccgccaaccc
     7201 tattgaccca cggaccttcg ttgggtactc tctcccgctc gaagtcggcg tgttggttga
     7261 ttctgcagat cggggcccgc tcgcgcgcgc ccccgcgcag cacgcgcgcg gaggccggaa
     7321 gtgaccgaca aggaacccaa caaggtcacg gcctggcggc aggtcggtct tctgacgtcg
     7381 attccgttca ttctcgcgct ggctcccatc gtcggatact tcctcgggaa attcctcgac
     7441 cagaagttcc gaacgcagcc ctggctgagc ttcatcctgc tggtgctggg gtttgtggcg
     7501 ggagtccgcg agacgatcca catcgtcaag ttgtcgcagt cggaggattg acccccctga
     7561 tccgtaacga aaacgccgca acgaatccgc tcgctcgcgc gatctggatg tcctggcttg
     7621 ccgccttcgc gctggccggg ctcctccttc cgcgcactcc ggggatcgcg ctcgcgatcg
     7681 tcgccggaca gagcctgatg gcgatcaact tcctctttct cgacaagctt gtgaaactct
     7741 tcacacacgc gatgctgccg ggcacaggca accgtcagat ccccgggcgc ttcttcgggt
     7801 acgcgtcgct caagatcctg ctcgtgtacg gcggcgcgtt cgctctgatt cgaagcggcg
     7861 ccgttccgtt cctgccgctc gtgggcggtc tctcgcttcc gcttctcgtt ctcttcctcc
     7921 agtcactcat gtgcgccgcg gggtacgcgg cctctccgtt ttcgcggcgg gcacccaaaa
     7981 cggcggctac cttcgggaat gccgctaagc ctgccgcgtt cttcttcatc ctcgctcttc
     8041 tcgcggttcc cgctctgggt cacgcttcgg acgacggcgc caaagcggaa ccggtcgttg
     8101 tcgcgcagca cgatcccgcg cagccgacca cgcccgccaa tccggacatg gcgacgcaga
     8161 gcgatgcggc cgcgacggcg cacggcgggg ccgccgggca cggcgaggcc gcgtcaggcg
     8221 agcacgccga ggggggcgag gcgcacctgc cgaactgggt cgtcatcctc aacgattact
     8281 tcccgaacaa tccggtcgtc cactggatgc acgagaacga gtacctcgtg ttcgcgtggc
     8341 tcgcggggct cgtgttcttc gtcgtggtct ggatcgcgat gcggaagccg accctcgtcc
     8401 cgggcacgct gcagaacggg atcgagtgga tcgtggagag catggaggac gtctgcggcg
     8461 ggatgctggg gaagcacctt cccaagtact tcccgttcat catggcgatc ttcttctaca
     8521 tcctgaccat gaacctgatc gggatcatac cgggcttcaa gtcctcgacc tccacgctgg
     8581 acgtgacgct ggcgctcgcg ctcatcacgt tcctctacgt ccagatcaac ggcatcatca
     8641 acctggggcc gctcggctat ctcgaccacc tcgccggctc gccgcgcgac atcgtgggct
     8701 tcgcgatgct ccccgtgatg atcccggtgc acatcctggg cgagctggtg aagccggtga
     8761 gcctctcctg ccggctcttc gggaacatct tcggcgagga cacgctgatc gtggtcttcg
     8821 tcggcgtcgc cgcgctcgcg ctcaaggcga gcctgaccct gctcgcggtg cctccgatgg
     8881 ccgggatcac ggcgctattc atgttgctcc agacgctcac ggccatcgtc caggccttga
     8941 tcttctcgct gctcaccacc gtctacctgt acatgatgct tccgcacgag gagcacgcgc
     9001 acggggaagc cgggcacgaa cacgcgtaac cccaacccac ggaggaagca cagatgaatt
     9061 tcgccgccac tctcggcctc gcgctcccga tcgggatcgg tctggccgcc atcggttcgg
     9121 gcatcggtct cggcttcatg ggccgcggcg cgatggaggc catgggccgt cagccggaag
     9181 tgctgccgaa gatccaggtc gccatgatca tcggcttcgg attcatcgag gcgctcacga
     9241 tctacgcgtt cgtcacgatg ttcttcctcc agggaaagat ccagtaaccg atcggagccg
     9301 ggcgccacgc gcgcccctcc ggtccgggaa ggacctatga gccaattcgt ccactggaat
     9361 cagctcctgg cgcacgccct gagcttcgtc gtcttctact tcctcgtgcg catggcgttc
     9421 gcggcgatca tctatccgcc gatgaaggag cgccgcgaca agatcaaggc ggagttcgag
     9481 cgcatcgagt cggagaaggc ggccgcggag cggcttcgcc aggagtacga gggacacatc
     9541 aagcggatcg aggccgagag ccgcgagcgc atccagaacg cggtctccga agggcagaag
     9601 gtcgccgcgg agattcgcga gatcgcgcgg aaggaagccc aggacaccat ccagcgcgcc
     9661 aaggaggaga tcgcgcggga gcacgacaag gcggcggcca agctcaaggg cgaggtcgtg
     9721 gacctggcca tggagatcgc gggcaaggtc gtgcgcgagg agctgacggc cgacaagcat
     9781 cgccggctcg tcgatggatt cctgaccgag ctggagcaga cgcggtgatc tcggtcacgc
     9841 tggcccgccg ttacgcgcgg gcgctcctcg atctggcaca aaggcagaac gaggtcccgc
     9901 gcaccgaagc ggagctcaag acggtcgggg agctcttccg caaggatccc cgcgcccggc
     9961 gcttcttcga gaccccgaac atctcccgtg aggagaagac ccggttcctc gaagagcgcc
    10021 tgatgccgcg ggtgtgccgg ccgctctatg gactcttcca cgttcttctg cgccgtcggc
    10081 gtctcgacca tctcgtcgtg atcgccgagg agttcgagaa gctggccgag aaggcgcagg
    10141 gcatcacgca cgcgacggtg cgcacggcgg ctccgatcac ggacgcccag gcggaccggc
    10201 tcacgcaggt cctcgtgcgg cgcatgggag gcacgattca gctcacgcgc gaggtggacc
    10261 cgtccctgct cgggggcgcg gccgtt
//
LOCUS       DASTKC010000224         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_663481, whole genome shotgun sequence.
ACCESSION   DASTKC010000224 DASTKC010000000
VERSION     DASTKC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663481"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1152)
                     /gene="hisC"
                     /locus_tag="VFR25_09425"
     CDS             complement(52..1152)
                     /gene="hisC"
                     /locus_tag="VFR25_09425"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9941310.1"
                     /translation="MAPPAVDSIRSEVRSLQPYVPGKPIEELERELGIAGAIKLASNE
                     NPFGPSPLALQAIEETARYANRYPDGSSYYLREALSTHWGVPSEWIAVGSGSNDLIDI
                     LCRIHLGPQDEAIMSNPSFVMFAIAVKVAGGALVRVPGRDLHHDLGAMLDAVTERTKI
                     VYIANPDNPTGTIVGRDALENYFSRVPGHVLTILDEAYFEYVTDPKYPDGLEHLRRGR
                     RVAVLRTFSKIHALAGLRVGYGFFPPDLASMVHRVRLPFNVTTVGEAAARASLRDPEQ
                     VSRSRERNAAGLDYYRAELPKLGLELTPSWANFVLAKFPRSVLEISRALEREGVIIRP
                     MASFGLAHEYARISVGTVAENERLVEVLRRIV"
     gene            complement(1184..1927)
                     /locus_tag="VFR25_09430"
     CDS             complement(1184..1927)
                     /locus_tag="VFR25_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9941311.1"
                     /translation="MRLNQFLARGAGGSRREADGWIRDGRVLVNGAPPNGMGVDVDPA
                     QDRVTLDGSPVHLPKAHRYVAYHKPAGLLVSRRSQGGKRTIYESLGARIHGLHTIGRL
                     DHESEGLLLLTDDGDLSEALLHPRTGLVRRYRLWVTPVPDPSALRLLQDGAEVEGVHV
                     APIHVEHEGVDRGRGVIVMDLGEGRKREARRLAQAAGLGVTRLVRIRFGPIQLGTLRS
                     GMLRALTEAEIDALRRIAFRPGGRDATLR"
     gene            complement(1917..2612)
                     /gene="scpB"
                     /locus_tag="VFR25_09435"
     CDS             complement(1917..2612)
                     /gene="scpB"
                     /locus_tag="VFR25_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET9941312.1"
                     /translation="MTPTPKQILEALLFASDAPVPLSALVEVLDGLGPSEVSAILTEL
                     GREYEESERGVALAEIAGGYQILSRRECAPWIEEMLRSRRKARLSRAGLETLAIVAYK
                     QPITKVEIDGIRGVDSGGSLHTLLERNLVLIRGRSKGVGRPLLYGTTPDFLSYMGVNE
                     LSDLPELKELGSVLEERERVHGELEDVSPATTVEGPDLFHPEAVAESAGDAEPSSDAE
                     AKVEEAMSPEGAA"
     gene            complement(2609..3532)
                     /locus_tag="VFR25_09440"
     CDS             complement(2609..3532)
                     /locus_tag="VFR25_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9941313.1"
                     /translation="MSDGPDERAEIATETAPAIEAIATPAAAASTNGSHPEGHSSIRV
                     NVPAFDGPLDLLLHLIQQNEIDIRDIPIAKITRQYLETLELMRELDLEVAGEFLVMAA
                     TLMRIKARMLLPPAVTEEEEEDPRETLVRQLLEYSRFKEAAHGLMKLETERRLHWERG
                     APAQLEDPESRELLPVSMFRLLDALKGVLSRQVPAIVHTVHAEPISLEEAIGLMRTQL
                     GAKPKLLFEELLEAFVTRIEKITAFLGLLELLKQGTIHATQESLFGPIWIEWQGDQPL
                     LEETRQLEEPGHEPRHDEITQTEFAGEEVDT"
     gene            complement(3529..>4131)
                     /gene="trpS"
                     /locus_tag="VFR25_09445"
     CDS             complement(3529..>4131)
                     /gene="trpS"
                     /locus_tag="VFR25_09445"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET9941314.1"
                     /translation="HLGYPVLQAADILLYKANLVPVGEDQVPHVELTREIARKFNTLY
                     GEVFPVPEAKLTRFARVPGLDGRRMSKSLGNTVLLSDSPEEIAAKIRTAYTDPKKIRA
                     TDPGNPDGCVVFAYHRAFNADQAPRIDQLCRAGQLGCVADKKDLAQVLADQLAPFRER
                     RKELEAHPKRVVDALKHGEEKARAIALRTMAEVREAMKLP"
BASE COUNT          625 a         1385 c         1420 g          701 t
ORIGIN      
        1 cgagagcgat gccctcgcga ccgagaccga tgatgagaat ccggagatcg atcacacgat
       61 ccggcgaagg acctcgacca gccgctcgtt ctcggccacc gtgccgacgc tgatgcgcgc
      121 gtactcgtgg gcgaggccga aggaggccat ggggcgaatg atcacgccct cgcgctcgag
      181 cgctctcgag atctcgagga cggagcgcgg aaatttcgcg agcacgaaat tggcccagct
      241 cggcgtgagc tcgaggccga gcttcggcag ctcggcccgg tagtagtcga ggcccgctgc
      301 gttccgctcg cggctccggc tcacttgctc gggatcgcgg aggctggcgc gcgccgcggc
      361 ctcccccacc gtggtcacgt tgaacgggag ccgcacgcgg tggaccatcg acgcgagatc
      421 cggcgggaag aagccgtatc ccaccctaag gccggcgagc gcgtggatct tcgagaacgt
      481 ccgcagcacg gcgacgcggc gtccgcggcg caggtgctcg agtccgtccg gatacttcgg
      541 atcggtcacg tactcgaagt acgcttcgtc caggatcgtg agcacgtggc cgggaacgcg
      601 gctgaagtag ttttcgagcg catcccgccc gacgatggtg ccggtgggat tgtccggatt
      661 cgcgatgtac acgatcttgg tgcgctccgt cacggcgtcg agcatggcgc cgagatcgtg
      721 atggagatcc cgccccggca cccggacgag cgcgccgccc gcgaccttca ccgcgatcgc
      781 gaacatgaca aacgacggat tcgacatgat ggcctcgtcc tgcggaccga ggtggatcct
      841 acagaggatg tcgatcaggt cgttcgatcc ggagccgacg gcgatccact cggacggaac
      901 gccccagtgg gtgctcagcg cctcgcggag gtagtaggag ctcccgtcgg gatagcgatt
      961 ggcgtatcga gccgtctcct cgatcgcctg gagcgcgagc ggcgacggac cgaatggatt
     1021 ctcgttggag gccagcttga tggcgcccgc gatgccgagc tctcgctcga gctcttcgat
     1081 gggcttgccg ggaacgtacg gctggaggga gcggacttcg cttcggatcg aatcgactgc
     1141 cggcggggcc atcgggatct ctccgttccg cgtcagagta gcgtcatcgc agtgtagcat
     1201 cgcgccctcc cggtcggaaa gcgatccggc ggagcgcgtc gatctcggcc tcggtgagcg
     1261 cccggagcat tcccgagcga agggtgccca gctggatcgg gccgaagcga atccgcacga
     1321 gccgcgtcac gccgagcccc gcggcctggg cgaggcgccg tgcctcgcgc ttcctccctt
     1381 cgcccaggtc catgacgatg actccgcggc cgcgatcgac tccctcgtgc tccacatgga
     1441 tcggcgcgac gtgcacgcct tcgacctccg ccccgtcctg caagaggcgg agcgcggacg
     1501 gatccggcac cggagtgacc cagaggcgat agcggcggac gaggccggtc cgcggatgaa
     1561 ggagcgcttc cgagagatcg ccgtcgtccg tgaggaggag gagcccttcc gattcgtggt
     1621 ccagccgccc gatggtgtgg agaccgtgaa tgcgggctcc gagcgactcg tagatggtcc
     1681 gctttccgcc ctggctcctc cggctcacga gcagtccggc cggcttgtgg tacgcgacgt
     1741 agcggtgggc tttgggaagg tggaccggcg acccgtcgag cgtcacgcgg tcctgggcgg
     1801 gatcgacatc gactcccatg ccgttgggcg gtgcgccgtt cacgagcacg cgcccgtcgc
     1861 ggatccagcc gtcggcttcg cggcgcgagc ctcccgcgcc ccgagcgagg aattggttaa
     1921 gccgcaccct cgggactcat cgcttcctcg actttggcct cggcatccga ggacggctcg
     1981 gcgtcgccgg cagactccgc cacggcttcg ggatggaaga gatccggacc ctcgacggtg
     2041 gttgccgggg acacgtcttc cagctcgccg tgaacgcgct cgcgctcctc gaggacgctc
     2101 cccagctcct tcagctcggg gagatccgac agctcgttga cccccatgta cgagaggaag
     2161 tcgggggtgg tgccgtagag gagcggacgg ccaacgccct tcgatcggcc gcggatgagg
     2221 acgagattcc gctcgaggag cgtgtggagg gagccgccgg agtcgacgcc gcggatgccg
     2281 tcgatctcga ccttcgtgat cggctgcttg taggcgacga tggcgagggt ctcgagcccg
     2341 gcgcgcgaga gccgcgcctt gcgccgcgag cgaagcattt cctcgatcca tggagcacat
     2401 tcgcggcgcg agaggatctg atagccccct gcgatctcgg ccagcgccac gccgcgctcg
     2461 ctctcctcgt actcccgtcc gagctcggtg aggatcgcgg atacctcgga cggccctagg
     2521 ccgtccagga cctcgaccag cgccgagagc ggcaccggcg cgtcgctcgc gaagagaagg
     2581 gcctcgagga tttgcttcgg tgtcggcgtc acgtgtccac ctcctccccc gcgaattcgg
     2641 tctgcgtgat ttcgtcgtgc cgcggctcgt ggccgggctc ctccaactgc cgcgtctcct
     2701 cgagcagcgg ctggtcgccc tgccattcga tccagatcgg gccgaacagc gattcctggg
     2761 tcgcgtggat cgtcccctgc ttcaggagct cgaggaggcc gaggaacgcg gtgatctttt
     2821 cgatgcgcgt cacgaacgcc tcgaggagct cctcgaacag gagcttgggt ttcgcgccga
     2881 gctgcgtgcg catgagcccg atcgcctctt cgagcgagat cggctccgcg tgcaccgtgt
     2941 gcacgatggc cggcacctgc cgcgagagca cgcccttcag cgcgtcgagc agccggaaca
     3001 tgctcaccgg gaggagctcg cgcgattcgg gatcctcgag ctgcgccgga gcgccccgct
     3061 cccagtggag ccgccgctcc gtctcgagct tcatcaggcc gtgggccgcc tccttgaagc
     3121 ggctgtactc gaggagctga cgcacgaggg tctcgcgcgg atcttcctct tcctcctccg
     3181 tgaccgccgg agggaggagc atcctcgcct tgatgcgcat caaggtcgcc gccatcacga
     3241 gaaactcccc cgcaacctcg aggtccagct cgcgcatcag ctcgagcgtc tcgaggtact
     3301 gcctcgtgat cttcgcgatc gggatgtcgc gaatgtcgat ctcgttctgc tggatgagat
     3361 ggaggagaag atcgagcggg ccgtcgaagg ccgggacgtt cacgcgaatc gacgagtggc
     3421 cctcggggtg ggagccgttc gtcgatgcgg cggcagcggg cgtggcgatg gcctcgatgg
     3481 ccggagccgt ctccgtggca atctccgctc tctcgtccgg tccgtcgctc atggcagctt
     3541 catcgcctcc cggacttccg ccatcgtgcg cagcgcgatc gcgcgcgcct tctcctcgcc
     3601 gtgcttcagg gcgtcgacca cccgcttggg gtgcgcctcg agctccttcc gccgctcgcg
     3661 gaagggggcg agctggtccg ccagcacctg cgcgagatct ttcttgtcgg ccacgcagcc
     3721 cagctgtccg gcccggcaga gctggtcgat ccggggcgcc tggtccgcgt tgaaggcgcg
     3781 atgatacgcg aacacgacgc atccgtccgg atttcctgga tcggtcgcgc ggattttttt
     3841 cggatccgtg taggcggtgc ggatcttggc cgcgatctcc tcgggcgagt ccgagaggag
     3901 caccgtgttg ccgagcgact tgctcatccg ccgcccatcc aggcccggca cgcgcgcgaa
     3961 gcgggtcagc ttcgcctcgg gcaccgggaa gacctcgcca tagagggtgt tgaacttgcg
     4021 cgcgatctcc cgcgtcagct cgacgtgcgg cacctggtcc tcgcccaccg ggacgaggtt
     4081 cgccttgtag aggaggatgt ccgccgcctg gagaacggga tacccgagat g
//
LOCUS       DASTKC010000225         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_664306, whole genome shotgun sequence.
ACCESSION   DASTKC010000225 DASTKC010000000
VERSION     DASTKC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664306"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFR25_09450"
     CDS             complement(<1..299)
                     /locus_tag="VFR25_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HET9941315.1"
                     /translation="MSPTRNPNLEKYRQKRDPGRTPEPFGESSSGGAPGVAPRFVVQK
                     HWARAMHYDLRLELDGVLKSWAVPKGPSTRAEEKRLAVHVEDHPLEYGSFEGMIPA"
     gene            427..1293
                     /locus_tag="VFR25_09455"
     CDS             427..1293
                     /locus_tag="VFR25_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9941316.1"
                     /translation="MPPHSIGSGTLSFGLVSIPVKMFTAASSANVSFNLLHEKCGGRI
                     KQQMVCPACNVTVERSNLVRGYEFSKDQYVRFTDEELKSVEGEASEIIDIAEFVPLEQ
                     VDPIYFEKSYYLGPDKGGAKAYRLLCDAMEKSQKVALAKFVMRGKESIVLIRPSQDGL
                     MLHTMYFSDEVRDFNEIDKGSDASIRAGELDLAVKLIGELSTDSFKPDAYADEYRGRV
                     LAMAQEKIEGKEITATGPKVERTQVIDLMDALKQSLERRVGQEGKAAPKKPAAKAKRT
                     ATPTKESKRASR"
     gene            1319..2233
                     /locus_tag="VFR25_09460"
     CDS             1319..2233
                     /locus_tag="VFR25_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941317.1"
                     /translation="MDAAQHFSARDAARILQTTEAQVRAWARAGGIVPEPGASGSPEF
                     TFQQLILLRTTRGLYEAGIPPGQVKRVWASLRRQLTDDLPLTSIRILADGRRAVAWDG
                     HAPWQPDSGQFVLDFNAGEIAEHAGVKEEAKVAMLSVVVPLTEEKTASGPLVADPEPE
                     PASLTAEQWFHIACELDGISPLEAQHAYLQALRMDPEYADAHVNLGRLLHEAGELGRA
                     EAYYRKAAEHAPEDSTIPYNLAVLLEDRGRPEEAILAYRRAIDLDAEAADAHYNLGLL
                     LDSLGRRSDAMRHLMTARQLYARSGGGR"
     gene            complement(2257..>2544)
                     /locus_tag="VFR25_09465"
     CDS             complement(2257..>2544)
                     /locus_tag="VFR25_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9941318.1"
                     /translation="KNSQRLNGVEEVRRLKESKGPALHIWGSSNLLQSLIAAELIDEH
                     RLWVFPIVLGEGKRLFEEGVPPRGLTLTATGRTPSGVLLNTYQPAGDLPKA"
BASE COUNT          449 a          813 c          854 g          428 t
ORIGIN      
        1 gcggggatca ttccctcgaa cgacccgtat tccagcggat ggtcctctac gtgaacggcg
       61 aggcgcttct cttccgcgcg tgtcgagggt cccttcggca ctgcccaact tttgagcacc
      121 ccgtcgagct cgagcctcag gtcgtagtgc atcgcgcggg cccagtgctt ctgaaccacg
      181 aaccgcgggg cgaccccagg cgcgccccca gacgacgatt ccccaaaggg ttccggcgtg
      241 cgcccgggat cgcgcttttg gcggtatttc tcgagattgg ggtttcgagt cgggctcacg
      301 ggcgggtctg gagaggtcac ccagcgctaa aggcgggctc catgtgatcc gataaacagt
      361 gcactcacag ttgagtatag caaagggttg gtgctagcct cccggaagtc gccggaggac
      421 tctcgtatgc ccccacatag catcggttcg ggaacactgt cgttcggact ggtctcgatc
      481 ccggtcaaga tgttcaccgc cgcgtcgtcc gcgaacgtct cgttcaacct gctccacgag
      541 aagtgcggcg ggcggatcaa gcagcagatg gtctgccctg cctgcaacgt gaccgtggaa
      601 cggtcgaacc tggttcgcgg ctacgagttc tcgaaggacc agtacgtccg gttcaccgac
      661 gaggagctga agtcggtgga gggcgaggct tccgagatca tcgacatcgc cgagttcgtc
      721 ccgctcgagc aggtcgaccc catctacttc gagaagagct actacctcgg ccccgacaag
      781 ggcggcgcca aggcgtaccg tctcctctgc gacgccatgg agaagtcgca gaaggtcgcg
      841 ctcgccaagt tcgtgatgcg cggcaaggag tcgatcgtcc tcatccggcc gagccaggac
      901 gggctgatgc tccacacgat gtacttctcc gatgaggtcc gcgacttcaa cgagatcgac
      961 aaggggtcgg acgcctccat tcgcgccggc gagctcgatc tcgcggtcaa gctgatcggc
     1021 gagctctcga ccgattcgtt caagccggat gcatacgccg acgaataccg cgggcgcgtg
     1081 ctcgcgatgg cgcaggagaa gatcgaaggc aaggagatca cggccacggg cccgaaggtc
     1141 gagcggacgc aggtcatcga tctgatggat gcgctcaagc agagcctcga gcgtcgcgtg
     1201 ggccaggaag gcaaggcggc gcccaagaag ccggccgcca aggcaaagcg aacggccacc
     1261 cctacgaagg aaagcaagcg ggcatcacgt taacggcagc gcggggcggt ggatgagcat
     1321 ggacgcggcg cagcatttct ccgcgcgaga cgcggcccgg attcttcaga cgaccgaggc
     1381 ccaggtacgg gcctgggcgc gcgcgggcgg gatcgtccct gagcccggcg cgagcggctc
     1441 ccccgagttc accttccagc agctcatctt gcttcgaacc acccgcggct tgtatgaggc
     1501 cgggatcccg cccggccagg tgaagcgcgt ttgggcctcc ctgagacgcc agctcaccga
     1561 cgaccttccc ctcacttcga tccgcatcct cgccgacggc cggcgtgcgg tggcctggga
     1621 tggccatgcg ccatggcagc cggattcggg acagttcgtt ctggatttca acgcgggtga
     1681 gatcgcggag catgccgggg tgaaggagga ggcgaaggtc gcgatgctgt ctgtggtcgt
     1741 gcctttgacg gaggaaaaga cggcgagcgg cccgcttgtt gcggacccgg agccggagcc
     1801 tgcctctctg accgccgagc agtggttcca cattgcctgc gagctggacg gcatttctcc
     1861 gctcgaagcg cagcacgcgt atctgcaggc gctgcgcatg gatccggaat acgcggatgc
     1921 gcacgtgaat ctgggacgcc tgttgcacga ggcgggcgag ctggggcggg cggaggcgta
     1981 ctaccggaag gccgcggagc acgcgccgga ggattcgacc atcccctaca accttgcggt
     2041 gctattggag gatcgcgggc ggcctgagga ggcgatcctc gcgtaccggc gcgccatcga
     2101 tctcgacgcc gaggcagcgg acgcgcacta caatctcggg ctcctgctgg attcgctggg
     2161 acgtcgctcc gacgcgatgc gccacctcat gaccgcccgc cagctctacg cccgctccgg
     2221 cggcgggaga tagcccaccc cgcacctgct ccgagctcag gcctttggaa gatcgcccgc
     2281 aggctggtag gtgttgagga gaactccgct cggcgtgcgc ccggtcgcgg tcagagtgag
     2341 accgcgcggc gggacgccct cttcgaagag ccgctttcct tcgccgagaa cgatcgggaa
     2401 gacccagagg cgatgttcgt cgatgagctc cgcggcgatc agggactgca ggaggttgct
     2461 gctgccccag atgtgcaacg ccggaccttt cgattccttc agccggcgga cctcctccac
     2521 gccgttcagg cgctgggagt tctt
//
LOCUS       DASTKC010000226         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1611237, whole genome shotgun sequence.
ACCESSION   DASTKC010000226 DASTKC010000000
VERSION     DASTKC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611237"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFR25_09470"
     CDS             <1..522
                     /locus_tag="VFR25_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350019.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET9941319.1"
                     /translation="RRGIFGGMEYPSEQRAHLTYELPLGEIVLDFYDKLKSLSRGYAS
                     LDYELAGYKTSELVKMDVLLNGDMVDALSVIVHREKAYDWGREIALKLKELIPRQMFP
                     IAVQAAIGGQIIARETISALRKNVLAKCYGGDITRKRKLLEKQREGKQRMKQIGTVRI
                     PQEAFLAVLRVER"
     gene            541..1209
                     /gene="lepB"
                     /locus_tag="VFR25_09475"
     CDS             541..1209
                     /gene="lepB"
                     /locus_tag="VFR25_09475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9941320.1"
                     /translation="MAKVATTSAERARARTKSSIREYVEALVLAIVLTIVIRGLVIQA
                     FRIPTGSMEDTLLVGDFLFVNKMVYGSEIDIGFSGNRLLYYRFPAIRQPKVGDIIVFR
                     YPDDPARDFIKRCVAVGGQTVEIRDKVLYVDGHPREESYVVHKDPRVLPREVNQRDNF
                     GPFVVPQGHLFMMGDNRDNSQDSRFWGALPENLVKGKAMVLYWSWDADRRLPRLNRIF
                     RPIL"
     gene            1221..2381
                     /gene="hemW"
                     /locus_tag="VFR25_09480"
     CDS             1221..2381
                     /gene="hemW"
                     /locus_tag="VFR25_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET9941321.1"
                     /translation="MEHGLYIPLPYCRSLCPYCAFAKAPLHHAEPKRLLDALRREAEM
                     ARADAAWDRPRTVFFGGGTPTALEPADLHATLDWVRRDWDLKRAREWTVEANPEGLSA
                     EKLAILREAGVDRLSLGVQSLEPSVLRSLGRIHKPERALEAVADARRAGFTNVSVDLM
                     VAVPGETEDGVLRAITAFVDLGVPHLSIYSLQVEPGTPLAGKVERGLVTPLDQDTAAD
                     RYEQVGERLAAAGYRHYEVSNWARPGFESRHNEGYWTRRPYLGLGPGAHSFDGSARWR
                     NEEDVTRYYERVEAGELPREDRQAVDLESAATEMIFLALRRARGLRRRHAERLAGPAL
                     AGWSEWARSAGALRLDPPGRIRPTERGLLLSHEISSDLLARVGRIDSPAPRC"
     gene            2463..3479
                     /locus_tag="VFR25_09485"
     CDS             2463..3479
                     /locus_tag="VFR25_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941322.1"
                     /translation="MTESQLDERHRRILTFVVERHVSRAGPVGSQTIRAAYHLSISPA
                     TIRSAMQQLEEMGYLDHPHTSAGRVPTEAGFRLYVDELMKPEPLGSAVRRAVNEALTA
                     SPPEAEEADPRVPGMLARMARQLAVVAVSGARVIEGADLVLLDDGLLLLAWRETGSVV
                     ATSSWMPAGAPGAADVRRAEAAIRGLLPIRSAVEARAHAVRARREPGAAMAIVVEALE
                     RAAARLESEGRTDVRIEGADHIASQPEFQSPDRLRPLVSLLAEREPLARALEAGAGPG
                     RAKVSIGSENGPGAMRHCSLVAVRVEGRGMHRFVGVMGPVRMPYRRIVSLVTYVGERL
                     SESE"
     gene            3476..4054
                     /locus_tag="VFR25_09490"
     CDS             3476..4054
                     /locus_tag="VFR25_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9941323.1"
                     /translation="MMKRKNEVEPTESVSPEGVEPAGDLGPETSVEAAEGSSLITELE
                     RLRGREDELLRALAEQQNVVRRRRQEMESSLQYAQESLVKDLLPVLDDFERALKAMDG
                     AANDSMRAGVALVYERLVRILTAQGLEPIRPLGEPFDPTLHDALAQDPAPGKAPGTVV
                     EVAQPGYRFKGRVLRHAKVVVAGSAEPSEGGI"
     gene            4054..>4230
                     /locus_tag="VFR25_09495"
     CDS             4054..>4230
                     /locus_tag="VFR25_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET9941324.1"
                     /translation="MARRDYYEVLGVEKGASEDEIKKAYRQLAMKYHPDRNPGSKEAE
                     EKFKEVAEAYEVLHD"
BASE COUNT          693 a         1433 c         1480 g          624 t
ORIGIN      
        1 cgccgcggca tcttcggcgg catggagtat cccagcgagc agcgggccca tctcacgtac
       61 gagctcccgc tcggggagat cgtcctcgac ttctacgaca agctgaagtc cttgagccgg
      121 ggctacgcgt cgctcgatta cgagctcgcg gggtacaaga cgtcggagct cgtgaagatg
      181 gacgtcctcc tgaacggaga catggtggac gcgctctcgg tcatcgtgca ccgggaaaag
      241 gcgtacgatt ggggccggga gatcgcgctg aagctcaagg agttgatccc aaggcaaatg
      301 ttcccgatcg ccgtccaggc cgcgatcgga ggccagatca tcgcccgcga gacgatctcc
      361 gctctccgga agaacgtgct cgccaagtgc tacggcggcg acatcacccg gaagcggaag
      421 ctcctcgaga agcagcgcga ggggaagcag cgcatgaagc agatcggaac cgtgcgcatc
      481 ccgcaggagg cgttcctcgc cgtcctgcgc gtggagcgct agaccggagg aacgaggacg
      541 atggccaaag tcgcgaccac ctccgccgag cgcgcgcgcg cgcgaaccaa gtccagcatc
      601 cgcgaatacg tcgaggcgct ggtcctcgcg atcgtgctca cgatcgtgat ccgtggtctc
      661 gtcatccagg cgttccggat cccgaccggc tcgatggagg acacgctcct cgtgggcgac
      721 ttcctcttcg tgaacaagat ggtctacgga tcggagatcg acatcggctt cagcggaaac
      781 cgcctcctct actatcggtt cccggcgatc cgccagccga aggtggggga catcatcgta
      841 ttccgctatc cggacgatcc cgcgcgcgac ttcatcaagc gctgcgtggc tgtcggcggg
      901 cagacggtcg agatccggga caaggtcctc tacgtcgatg ggcatccgcg cgaggagtcg
      961 tacgtggtgc acaaggaccc gcgcgtgctt ccgcgcgaag tgaaccagcg ggacaacttc
     1021 gggccgttcg tcgtcccgca ggggcacctc ttcatgatgg gagacaaccg ggacaacagc
     1081 caggacagcc gcttctgggg cgcgctcccc gagaatctcg tgaaggggaa ggcgatggtc
     1141 ctctactggt cctgggacgc ggaccggagg ctcccgaggc tcaaccggat cttccgaccg
     1201 atcctctgac ggggcgtcgc gtggagcacg gcctgtacat cccccttccg tactgccgga
     1261 gcctctgccc gtactgcgcc ttcgcgaagg cgccgctgca tcacgccgag cccaagcggc
     1321 tcctcgacgc gcttcgccgc gaggcggaga tggcgcgcgc cgatgcggcc tgggaccgtc
     1381 cgcgcacggt cttcttcggc ggaggcacgc cgaccgcgct cgagcccgcc gatcttcacg
     1441 ccacgctcga ctgggtgcgc cgggactggg acttgaagcg tgcgcgcgag tggaccgtcg
     1501 aggccaatcc cgaagggctg agcgccgaga agctcgcgat tctgcgggaa gccggcgtgg
     1561 accggctgag cctcggcgtc cagtcgctcg agccttcggt gctccgatcg ctcgggcgca
     1621 ttcacaagcc ggagcgcgct ctagaggccg tggccgacgc gcgccgcgcg ggcttcacga
     1681 acgtctccgt cgatctcatg gtcgcggtgc ccggtgaaac cgaggacggg gtgctccgcg
     1741 cgatcacggc gttcgtcgat ctcggagtgc cgcacctctc gatctactcg ctccaggtcg
     1801 agcccggcac gccgctcgcg gggaaggtgg agcgcggcct agtgacgccg ctcgaccagg
     1861 acacggccgc cgatcgctac gagcaagtcg gcgagcgtct cgcggcggcg ggctaccgtc
     1921 actacgaagt ctcgaactgg gcgcgacccg gattcgaatc caggcacaac gagggctact
     1981 ggacgcggcg tccgtatctc ggtctcggcc ccggcgcgca ttccttcgac ggcagcgcgc
     2041 gctggcggaa cgaggaggat gtgacgcgct actacgagcg cgtcgaggcg ggggagctgc
     2101 cgcgcgagga ccgccaggcg gtggacctgg agagcgccgc cacggagatg atcttcctcg
     2161 ctcttcgccg cgcacgcggg ctccgaaggc gccacgccga gcggctcgcc ggccccgcgc
     2221 tggccggctg gtccgagtgg gcgaggagcg ccggagccct ccgcctcgat ccccccggcc
     2281 ggatccgtcc gacggagcgg gggctcctcc tctcgcacga gatctcctcc gacctcctcg
     2341 cccgggtggg tcggattgac agcccagccc ctcgctgcta gcctcacccc gttggcactt
     2401 cgaagggtcg agtgccaaat gatccgaatc caagcgacct gaatccaaac accccgagtc
     2461 ccatgaccga atcccagctc gacgagcggc accgccggat cctcaccttc gtggtggagc
     2521 gccacgtctc ccgcgccggc cctgtcggct cgcagacgat tcgcgccgcc taccacctca
     2581 gcatctcgcc cgcgaccatc cggagcgcca tgcagcagct ggaggagatg ggatatctcg
     2641 accatcccca cacctcggcg gggcgcgttc cgaccgaggc cggattccgt ctgtacgtgg
     2701 acgagctgat gaagccggag ccgctcggct ccgcggtgcg ccgggccgtg aacgaggcgc
     2761 tgaccgcgtc gcctccggaa gcggaggagg ccgacccgag agtgcccggc atgctcgcgc
     2821 gcatggcccg ccagctcgcg gtggtcgcag tgagcggcgc ccgcgtgatc gagggggcgg
     2881 acctcgtgct cctggacgac gggttgctcc tcctcgcctg gcgcgagacc ggaagcgtcg
     2941 tggccacgtc gagctggatg cccgcgggag cacccggcgc ggccgacgtt cgtcgcgcgg
     3001 aggcggcgat ccgcggcctt ctgcccatcc ggagcgcggt ggaggcgagg gcgcatgcgg
     3061 tccgcgcgcg gcgcgagccg ggcgccgcca tggcgattgt ggtcgaggcg ctcgaacgcg
     3121 cggcggctcg actagaatcg gagggacgca ccgacgtgcg gatcgagggg gccgatcaca
     3181 tcgcgtcgca gcccgagttc cagtcccccg accgactccg tccgctcgtt tcgctcctcg
     3241 cggagcggga gccgctcgcc cgcgccctcg aggccggcgc ggggccggga cgcgcgaagg
     3301 tctcgatcgg atcggagaac gggccgggcg ccatgcgcca ctgctcgctc gtcgcggtga
     3361 gggtcgaggg gcggggcatg caccgcttcg tcggcgtgat ggggccggtg cgcatgccct
     3421 atcgacgaat cgtgtcgctc gtcacctacg tgggcgagcg gctttcggag tccgaatgat
     3481 gaaacgaaag aacgaagtcg aacccacgga atccgtgtcg ccggaggggg tagagccagc
     3541 gggcgatctc ggacccgaaa catcggtcga ggccgccgag gggagctcct tgatcaccga
     3601 gctcgaacgg ctccgcggac gcgaggacga gctcttgcgc gctctcgccg agcagcagaa
     3661 cgtcgtgcgc cgccggcgcc aggagatgga gtcgtcgctc cagtacgcgc aggagtcgct
     3721 ggtgaaggac ctcctcccgg tgctcgatga tttcgagcgc gcgctcaagg ccatggacgg
     3781 cgctgcgaac gactccatgc gcgcgggcgt cgcgctcgtg tacgagcgcc tggtccgcat
     3841 cctcacggcg caggggctcg agccgatccg tccgctcgga gagcctttcg atcccacgct
     3901 gcacgacgcg ctggcccagg atcccgcgcc gggcaaggcg cccggaaccg tggttgaggt
     3961 ggcgcagccc ggctatcgat tcaaggggcg cgtgctccgc cacgcgaagg tcgtggtcgc
     4021 gggaagcgct gagccctccg agggcgggat ctgatggcgc gacgcgatta ctatgaagtt
     4081 ctcggcgtcg agaagggcgc atcggaagac gaaatcaaga aggcctaccg ccagctcgcg
     4141 atgaagtacc acccggaccg caatccgggg agcaaggagg ccgaggagaa gttcaaggag
     4201 gtcgcggagg cttacgaggt cctccacgat
//
LOCUS       DASTKC010000227         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2085470, whole genome shotgun sequence.
ACCESSION   DASTKC010000227 DASTKC010000000
VERSION     DASTKC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085470"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFR25_09500"
     CDS             complement(<1..1147)
                     /locus_tag="VFR25_09500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941325.1"
                     /translation="MTVARDPSTAYALGGGPTQEVAAPRGGWLERYEQLVGARTLRAL
                     EALARKLKGHRIVMVNTTKTGGGVAEILHRVVVILNELGIQTTWEVMEGDEQFFGVTK
                     KMHNALHGHTQPLTDEDREVYHERTRMEADRLALDGDLILIHDPQPASLIEHRREPGQ
                     HWVWRCHIDLSRRDEAYWDFLKPAVSRYDTAIFSHIEFVPPLTVPAVLVPPSIDPFAD
                     KNRDMAEDEIESICERLGLTATSPWITQVSRFDRIKDPIGVLEAFKLVRARRPSHLVL
                     AGGSASDDPEGAEVLAEVRQKAKDVKDVTILELPPQSDIEINAIQRASTVVLQKSLRE
                     GFALTVSEALWKRRAVVASAVGGIPLQVLHERTGLLVRSIEGTAFQTI"
     gene            complement(1144..1914)
                     /locus_tag="VFR25_09505"
     CDS             complement(1144..1914)
                     /locus_tag="VFR25_09505"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HET9941326.1"
                     /translation="MDQAALASVSESFTFYTPSPLESPAGQIATDIQSLRDGIAACSD
                     ASLFQHVTRMPVRHPHARDVPENDFARWAGDAMQMPEVSERLAFVGSGSCTSFDELRG
                     SLLGVLDRVQPKEKRREAPEGAAFHFLEAHSVLAPLGTEARTPAEVIEVWPWIDLGSA
                     FYHLVEAPLRGTHEHALIPWLRAHGGSSMADSAEQVVRSSRPLNILLKEVGARWRRSM
                     IGRRLIERAEAPELERQREARAAMARLAGRLRGRGNKQ"
     gene            complement(1887..>2493)
                     /locus_tag="VFR25_09510"
     CDS             complement(1887..>2493)
                     /locus_tag="VFR25_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941327.1"
                     /translation="AGAIALIGWIVGALLSRAAQGLLSLAGIDGPAKRLAAEGGIRVD
                     FLPSRMVGFSIFWLVILGTAIIALRVIGLDLAPSLVARLQDVLPRIVTSGLVLLLGIP
                     LALAAGRLLNALLIQSGVRPNRIREQSTGALLVAFTVLIALDQLGLAAHLVLAVVIAV
                     VAAAGLAVALAFGLGCRDLARDLIVEYLRASDEWTRPHSRP"
BASE COUNT          405 a          856 c          861 g          371 t
ORIGIN      
        1 ggatcgtctg aaacgcggtg ccttcgatcg accgcacaag taatccggtc cgctcatgca
       61 acacctgcag cggaatgccg ccgaccgcgc tcgcgacaac ggcccgccgc ttccacagcg
      121 cctccgatac cgtcagcgcg aatccctccc gcagcgactt ctgcagcacc accgtcgacg
      181 cacgctggat cgcgttgatc tcgatgtccg actggggtgg caattccaag attgtcacgt
      241 ccttcacgtc cttggccttc tggcgcacct ccgccaggac ctcggctcct tccggatcgt
      301 cgcttgcgct cccgcccgcc agtaccaaat gcgacggcct ccgggcccgc acgagcttga
      361 acgcctcgag cacgccgatc ggatccttga tccggtcgaa ccgcgacact tgggtaatcc
      421 acggagatgt ggccgtcaag ccgaggcgct cgcaaatgct ctcgatctcg tcttcggcca
      481 tgtcccggtt cttgtccgcg aagggatcga tggatggcgg cacgagcacg gccggcacgg
      541 tcagcggcgg cacgaactcg atgtgcgaga agattgcggt gtcgtaacgc gacacggcgg
      601 gcttcaggaa gtcccagtac gcctcgtccc gccgcgagag atcgatgtgg cagcgccaca
      661 cccagtgctg gccgggctcg cgccggtgct cgatgagcga tgcgggctgg ggatcgtgga
      721 tcaggatgag atcgccgtcg agcgcgaggc ggtcggcctc catgcgcgtc cgctcgtggt
      781 agacctcccg gtcctcgtcg gtcaggggct gcgtgtggcc gtggagggcg ttgtgcatct
      841 tcttcgtcac gccgaagaac tgctcgtccc cctccatcac ctcccacgtc gtctggatgc
      901 cgagctcgtt caggatgacg accacgcgat ggaggatctc ggcgacgccg cctccggtct
      961 tggtggtatt gaccatcacg atgcggtggc ccttgagctt ccgcgcgagc gcctcgagag
     1021 cccggagcgt gcgggcgccg acgagctgct cgtagcgctc gagccagcct ccgcgcggag
     1081 cggccacctc ctgtgtgggc cctccgccca gggcgtaggc ggtgctggga tcgcgggcga
     1141 cggtcattgc ttgttccccc gtccgcgtaa gcgcccggcg agacgagcca tggccgcgcg
     1201 cgcctcacgc tgcctctcga gctccggcgc ctcggcgcgc tcgatcagcc ggcggccgat
     1261 catcgagcgg cgccatcgcg cgccgacctc cttcaacagg atgttgagcg gtcgggatga
     1321 gcggaccacc tgctccgccg agtcggccat gctcgatccg ccgtgcgcgc ggagccaggg
     1381 aatcagcgcg tgctcgtggg tgcctcgaag cggggcctcg acgaggtggt agaaggccga
     1441 gccgaggtcg atccagggcc agacctcgat gacttcggcg ggcgtccggg cttcggtgcc
     1501 gaggggcgcg agcaccgaat gcgcttcgag gaaatggaag gctgcgcctt ccggcgcctc
     1561 ccgccgcttc tccttcggct gaacgcgatc gagtaccccg agcagcgagc cgcgtagctc
     1621 gtcgaagctg gtgcacgaac ccgaccccac gaaggcgagc cgctcgctca cctccggcat
     1681 ctgcatcgcg tcgccggccc agcgcgcgaa gtcgttctcc ggaacgtcgc gcgcgtgcgg
     1741 gtggcggacc ggcatccggg tcacgtgctg gaacagagac gcatccgagc aggcggcgat
     1801 cccgtcgcgg agcgactgga tgtcggtcgc gatctgcccc gccggcgact cgagaggtga
     1861 cggcgtatag aaggtgaacg attcgctcac ggacgcgagt gcggcctggt ccattcgtcc
     1921 gaagcgcgca ggtactcgac gatcaagtcg cgcgcgagat ccctgcagcc gagcccgaac
     1981 gcgagcgcga ccgcgagtcc ggccgcggcg accaccgcga tgaccaccgc cagcacgagg
     2041 tgcgcggcga ggccgagctg atccaaagcg atcagcaccg tgaaggcgac gaggagcgcg
     2101 ccggtgctct gctcgcggat ccgattggga cgcacgcccg actggatgag gagagcgttc
     2161 agaagccgcc ccgcggcgag cgcgagcggg atgccgagga ggagcacgag tcccgaggtc
     2221 acgatgcgcg ggagcacgtc ctggagccgc gccacgagcg acggcgcgag atcgaggccg
     2281 atcacgcgga gcgcgatgat cgcggtgccc aggattacca gccagaagat ggagaagccg
     2341 accatgcggc tcggcaagaa gtccacccgg atcccgccct cggcggcgag gcgcttcgcg
     2401 ggcccatcga tcccggcgag ggagaggaga ccctgggccg cgcgcgagag gagcgcgccg
     2461 acgatccagc cgatgagcgc gatggcccca gcg
//
LOCUS       DASTKC010000228        10588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1847806, whole genome shotgun sequence.
ACCESSION   DASTKC010000228 DASTKC010000000
VERSION     DASTKC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847806"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /gene="dnaX"
                     /locus_tag="VFR25_09515"
     CDS             complement(<1..1807)
                     /gene="dnaX"
                     /locus_tag="VFR25_09515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9941328.1"
                     /translation="MPHLALARKYRPQRFRDLIGQEPIRLTLERAVATDRVAHAYLFA
                     GPRGSGKTTTARLLAKALNCERRKPGESEPCNECGSCREITAGTSLDVLEIDGASNRG
                     IEEIRNLRENVKYAASGGKSKVYIIDEAHQLTDFAWNALLKTLEEPPAHVRFVFATTE
                     ALEVPDTIASRCQVFEFRRLKSEELVQHLTSVAKAEQVKLDPDAAALIARASEGAVRD
                     ALSRLDQALAVSPDGITAAVVAQVLGLAGLDAYFDLGAAIGARDAKAALETLDRLHDA
                     GMDVEELADGLTHHLRQLLLLAVDASLDKMIDAAPADRERYAAQAKAFRAGDLSAMLG
                     RLIESRAQLRRAEAPRVLLEVCLVELCTMPVASDVGDLIQRLQDLESRLGGGTPAAPR
                     SSTPSARPSAPTRPPAPPRREGTSGFEASAPARPSSDPPRAAEPPRSAPPREELTQDA
                     PRRPMMVDPIAPPSADLAAADEPSLADSALAARWREIVDRVKERKLLLGTCLEEGLFL
                     GVQGSSVRVALTAEHSFHRAMLEMKENKDILNQEFERTYGPGATLLCVGSDQAVTGAG
                     IRPAPREATQAAPAPSGLVQRIVELFDGEILGQGPE"
     gene            complement(1867..1966)
                     /gene="ffs"
                     /locus_tag="VFR25_09520"
     ncRNA           complement(1867..1966)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR25_09520"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2036..2111)
                     /locus_tag="VFR25_09525"
     tRNA            complement(2036..2111)
                     /locus_tag="VFR25_09525"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2076..2078),aa:Ala,seq:cgc)
     gene            complement(2134..2223)
                     /locus_tag="VFR25_09530"
     tRNA            complement(2134..2223)
                     /locus_tag="VFR25_09530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2187..2189),aa:Ser,seq:gga)
     gene            complement(2272..2361)
                     /locus_tag="VFR25_09535"
     tRNA            complement(2272..2361)
                     /locus_tag="VFR25_09535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2325..2327),aa:Ser,seq:cga)
     gene            complement(2386..2871)
                     /gene="tadA"
                     /locus_tag="VFR25_09540"
     CDS             complement(2386..2871)
                     /gene="tadA"
                     /locus_tag="VFR25_09540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET9941329.1"
                     /translation="MIGQSPSDERFMQAALAEAETAESEGEVPVGAVVVLEGRIIGRG
                     HNRVEAAQDPTAHAEIIAIGAAAQTLNTWRLDGATLYVTLEPCHMCAGAIVLARIARL
                     VYGARDPKAGACGSLAMVPQDLRLNHRAEVVPGVLAEDASLLLEQFFRARRRATNRLE
                     T"
     gene            complement(2868..3539)
                     /locus_tag="VFR25_09545"
     CDS             complement(2868..3539)
                     /locus_tag="VFR25_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941330.1"
                     /translation="MRTHRLIRSIAAASALALLLATSLGAKKKDDPPPAKSIRVAIMP
                     VINGSAEVGAPKIMEDILHDRLKEIPAEQATFLQPTDTERLLEVHNASDKLFALNDKW
                     SNYGALDTTAVGGIDSLLNVDAILFVKVSEWENVRVNVIGRGESNTTVGFQFALFDLA
                     TKKRTWFKEPREQRFANEVDVSSGAVNYDETGYIQSRKVTDPPRYEDVASDLVRNAFK
                     KFPRQ"
     gene            complement(3598..3674)
                     /locus_tag="VFR25_09550"
     tRNA            complement(3598..3674)
                     /locus_tag="VFR25_09550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3638..3640),aa:Arg,seq:acg)
     gene            complement(3701..3792)
                     /locus_tag="VFR25_09555"
     tRNA            complement(3701..3792)
                     /locus_tag="VFR25_09555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3756..3758),aa:Ser,seq:gct)
     gene            complement(3848..4813)
                     /locus_tag="VFR25_09560"
     CDS             complement(3848..4813)
                     /locus_tag="VFR25_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9941331.1"
                     /translation="MAIRHRHEIHSERYDRGMRSSAIGLVLNFLLGVGKLAAGLLGHS
                     QALIADAIESLGDSVGSIIVWRGLQVASQPPDMEHPYGHGKAEPIAAALVAVLLIVAA
                     IGIAAESIHQIVIPHMSPAPFTLVVVIGVVVVKELLFRFTSRVSQETGSHTVRTDAWH
                     HRSDAITSGAAAIGIAVALIGGRGFEAADDWAALFASGIIVVTGWRLLRPAVNELMDQ
                     APPYDLVAETQRVAESCRGVYRVEKLLMRKMGIYYVADMHLEVDPDMSVRESHALAHG
                     VKEDIQRQLPQVVEVTIHVEPAWALSGGKPPDLGEAGRPGSGAPS"
     gene            complement(4818..5678)
                     /locus_tag="VFR25_09565"
     CDS             complement(4818..5678)
                     /locus_tag="VFR25_09565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9941332.1"
                     /translation="MRRGRLLIAAIVAVISLISYYSLRQENPFTGEVQHISMDPEQEI
                     ALGLQMAPQMAQEMGGEDDDATVRRVVEQVGQRVVAQGGASSTPYQFQFHALADPQTV
                     NAFALPGGQIFITHGLLARLENEAQLAGVLAHEVGHVVARHSAEQISKSELAQGLVGA
                     AGIAGSSDYDSGQHSAQVAALVAQMMQLRYGRQDETQADTLGVRMMAGAGYDPRAMIV
                     LMQILEQASGGGSQPEFMSSHPDPGNRRQVIEAAIHRNFPNGVPSDLSLGASFASGSS
                     EPEPQASTEP"
     gene            complement(5767..6666)
                     /locus_tag="VFR25_09570"
     CDS             complement(5767..6666)
                     /locus_tag="VFR25_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HET9941333.1"
                     /translation="MAHPIAVAFIHGIGRTEPGYSVPMQRSIQSAFEERTADAVAPAL
                     VFEEINWSAALQNREDLLYERLEEAGKLGWARLRHFMVDFAADAIAYQPAPSDRSAYD
                     AVHLEVACSLKRLAERAGPRAPLCIIAHSLGTVIASNYVYDLAKKRKSFACAEVRSAV
                     GRTPIERGETLALLYTLGSPIALWSLRYPKFGQPIAFPPRRLVRHHPDLAARWINVYD
                     PDDVIGYPLRELNSGYRRAVTEDRPVSAGSLWTGWTPLSHVGYWSNDRLAVSIATDLA
                     VSYLRASAVAPPHERPAPLLRQA"
     gene            6831..7094
                     /locus_tag="VFR25_09575"
     CDS             6831..7094
                     /locus_tag="VFR25_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941334.1"
                     /translation="MRRRAARVIAVLVDALQIVVFPAFFPGIVSPFDNALDVLTGIVM
                     TFLLGFHVAFLPTFIAELVPFVDLFPSWTLAVLFVTRKKPLKE"
     gene            7133..7573
                     /locus_tag="VFR25_09580"
     CDS             7133..7573
                     /locus_tag="VFR25_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9941335.1"
                     /translation="MAIARMANALNRALGVDVDSFTPERVREDAFGPMPLVSILVGEV
                     DGGAAGYAFYHTGYDSDVAARNLVVVDLYVEEEARGRGLGQALMAGLAREALRLGARS
                     MTGGVLDSNVKALAFYRRLGANQDGVRFVTFPEGALRALAEGTG"
     gene            complement(7596..8048)
                     /locus_tag="VFR25_09585"
     CDS             complement(7596..8048)
                     /locus_tag="VFR25_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9941336.1"
                     /translation="MTARRRETRTTARLALFDSRGRILLFLHADGLGRKFWATPGGGI
                     EEGESPEQAARREAAEELGASDVELTYLWTSFTEFEFADKDVSQTEAFFRVTGHPGTF
                     GPEVEAIHRRERILENRWWSAEEIEASEERILPLDLAKRVREFGGTPA"
     gene            complement(8045..8563)
                     /locus_tag="VFR25_09590"
     CDS             complement(8045..8563)
                     /locus_tag="VFR25_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941337.1"
                     /translation="MLRPPLPMTWLWCGLLLVVLGCGRSQPGAGTATRRPPESSSREA
                     PVDTLREPSNDEAAGVIRAYYDAINARHYSDAYVLWSGRGERSGQTVESFTVGFAQTD
                     SVRADIGAPSDMEAAAGSRYITVPVRLLAYEGKRTQSYRGTYTLRRAVVDGATAEQRQ
                     WRIAGAAIRRSR"
     gene            complement(8539..8886)
                     /locus_tag="VFR25_09595"
     CDS             complement(8539..8886)
                     /locus_tag="VFR25_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941338.1"
                     /translation="MEHEVVDGVPVPEGMGPEDRKNRRWSGFWFGMKLHLIVGIPVLL
                     VAAALRQLPPAAATFCLWQWLYLAPAIVFEFTHDRPKRARGILAAGLTTMALVTLLFF
                     LAIIILCFGPRFR"
     gene            complement(8886..10307)
                     /locus_tag="VFR25_09600"
     CDS             complement(8886..10307)
                     /locus_tag="VFR25_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9941339.1"
                     /translation="MADKVRPYLFYDIAISICSRCFRKVEGKVLFEDGKVYLKKRCPE
                     HGWECSLIADDIDYYKRCREIFLKPPEQPNRYNTPIRYGCPYDCGLCPDHEQHSCLAL
                     VEVTDHCNLTCPICYASSGPHRPGHRSLAEIERMLDAVVANEREPDVVQISGGEPTTH
                     PEFFAILDAARRRPIRHLMVNTNGIRIAREPGFAERLAAYMPGFELYLQFDSFERAAL
                     QDLRGADLRSIREGALSVLNDLDLSTTLVVTVKRGVNDGELGAILEFAAAQRCVRGVT
                     FQPVQAAGRLEGFDPEHNRLTLTEVRRRILEQTNLFRPEDLIPVPCHPDCLAMAYALK
                     SNQGLIPLTGLIDPQVLIEGGRNTIIYEQDRTLQAEVFRLFSTNHSPDSSARSLRDLL
                     CCLPRVAAPESITYRDVFRVIIMQFLDAHSFDVRSVKKSCVHIVHPDGRLIPFDNYNL
                     FYRDGLESTRLEPLRSLALAGVS"
     gene            complement(10297..>10588)
                     /locus_tag="VFR25_09605"
     CDS             complement(10297..>10588)
                     /locus_tag="VFR25_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941340.1"
                     /translation="VLIPIAIWAARRLRAGVMDGSIFRGFLIVYLAFRVALDFVKPEP
                     RPYLGLTATQIVGTIVIAYLVWVAQRSRAERVAGARATPDPIPPLAETAHGR"
BASE COUNT         1699 a         3496 c         3638 g         1755 t
ORIGIN      
        1 cttcgggccc ttggcccagg atctcgccgt cgaagagctc cacgatgcgc tggacgagcc
       61 cgctcggagc gggcgccgcc tgggtcgctt cacgcggcgc gggacggatc cccgcacccg
      121 tcacggcctg atcgctgccc acgcagagga gcgtagcacc cggcccatag gttcgctcga
      181 actcctggtt caggatgtcc ttgttctctt tcatctcgag catcgcgcgg tggaaggaat
      241 gctccgcggt gagcgcgaca cggacgctcg agccctgcac gccgaggaac aatccctcct
      301 cgaggcaggt gccgaggagg agcttccgct ccttgacgcg gtcgacgatc tcgcgccagc
      361 gggccgcgag cgcggagtcg gcgagcgacg gctcgtccgc ggccgcgaga tcggcggacg
      421 gaggcgcgat gggatcgacc atcatcgggc gccgcggcgc atcctgcgtc agctcctcgc
      481 gaggcggcgc ggaccgtggc ggctcggccg cgcgcggcgg atcggacgac ggccgcgcgg
      541 gggcgctcgc ctcgaagccg gacgtcccct cgcgtcgcgg aggcgcgggc ggccgcgtcg
      601 gagccgatgg ccgggccgag ggcgtcgagg agcggggtgc ggccggcgtg cctccgccga
      661 gcctcgattc caaatcttgc agccgctgga tgagatcccc cacgtcgctc gcgaccggca
      721 tcgtgcagag ctccacgagg cagacctcga ggagcacgcg cggcgcctcg gcgcggcgga
      781 gctgcgcgcg ggactcgatc agccggccga gcatcgcgct caggtccccc gcacggaacg
      841 ccttggcttg cgccgcgtat cgctcgcgat ccgccggcgc cgcgtcgatc atcttgtcga
      901 gcgaggcatc gaccgcgagg aggaggagct ggcgaaggtg gtgcgtgaga ccgtcggcca
      961 gctcctcgac gtccatcccc gcgtcatgga gccggtcgag cgtctcgagc gcggccttcg
     1021 cgtcgcgcgc cccgatcgcg gcgccgaggt cgaagtaggc gtcgagtccg gcgagtccca
     1081 gcacctgcgc cacgacggca gccgtgattc cgtccggcga gaccgcgagc gcctggtcga
     1141 gccggctcag cgcgtcgcgc acggctccct cggaggcccg cgcgatgagc gccgccgcgt
     1201 cgggatcgag cttcacctgt tcggccttcg ccacgctcgt gaggtgctgg accagctcct
     1261 cgctcttcag acggcggaac tcgaacacct ggcagcgcga agcgatggtg tcgggcactt
     1321 cgagcgcctc ggtggtggcg aagacgaacc gcacatgggc cggcggctct tcgagcgtct
     1381 tcaagagcgc gttccaggcg aaatcggtga gctggtgcgc ctcgtcgatg atgtagacct
     1441 tggacttccc gcccgaggcc gcgtacttca cgttctcgcg gaggttgcgg atctcctcga
     1501 tcccgcgatt ggacgcgccg tcgatctcga gcacgtcgag cgacgttccg gccgtgatct
     1561 cacggcagga gccgcactcg ttgcagggct cggactcgcc cggcttccgg cgctcgcaat
     1621 tcaacgcctt ggccaggagg cgcgcggtgg tcgtcttccc gctgccgcgg gggccggcga
     1681 agagataggc gtgggcgacg cggtcggtcg cgaccgcgcg ctcgagcgtg agacggatcg
     1741 gctcctggcc gatcagatcg cgaaaccgct gcggacgata cttgcgcgcg agcgcgagat
     1801 gcggcacggg ctacctcggg gtcaagacag gaccggagcc tgaagcaaag cccccccgcg
     1861 accggctcgg gtcagccgtg tccgcggcac atcgagtcga tcactgaccg ctgctacctt
     1921 ccggtcctga cggggttcgt gactctcgat tgcgcgggaa ctgaccgtca acgccaagcc
     1981 acagggaggc cagtgccgcg tcgaaaggcc ttcggctcag gtgcgtcacg atgcgtggtg
     2041 gagatgaccg ggatcgaacc ggtgacctcc tcgttgcgaa cgaggcgctc tcccaactga
     2101 gctacatccc cacgtgtcga ttcacttcgg aactggcgga gagagaggga ttcgaaccct
     2161 cgaagcactt tcatgcttac acggtttcca accgtgctcc ttcagccact cggacatctc
     2221 tccggggagc ctagggcgca cgcgcgctcc ctcgcgcgcc atatcacaag atggcggaga
     2281 gagagggatt cgaaccctcg atacgcttgc gcgtatagcg gttttcgaga ccgcccgatt
     2341 cagccactct cgcatctctc cgccgcgaga atacgcgaat cgggctcagg tttccaggcg
     2401 gttcgtggcg cgccgccgcg cccggaagaa ctgttccaga agcaagctag cgtcctctgc
     2461 gagcacgccc gggaccacct cggcgcggtg gttcaggcgg aggtcctggg gcaccatggc
     2521 gagcgatccg caggcgccgg ccttggggtc gcgcgcgccg tagacgagcc gggcgatccg
     2581 cgcgaggacg atcgccccgg cgcacatgtg gcagggctcg agggtgacgt agagggtggc
     2641 cccatcgagg cgccaggtgt tgagggtctg cgcggcggcg ccgatggcga tgatctcggc
     2701 gtgcgcggtg ggatcctggg ccgcctcgac ccggttgtgg ccgcgcccga tgatccgtcc
     2761 ttcgagaacg accaccgcgc cgaccggtac ttccccctcg gactccgccg tctcggcctc
     2821 ggcgagcgcc gcctgcatga agcgctcgtc cgagggggac tgcccgatca ttgcctcggg
     2881 aatttcttga acgcgttccg gacgaggtcg ctcgcgacgt cctcgtagcg gggcggatcg
     2941 gtcactttcc gcgactggat gtagcccgtc tcgtcgtagt tgaccgcgcc gctcgagacg
     3001 tcgacttcgt tggcgaagcg ctgctcgcgc ggttccttga accacgtccg cttcttcgtc
     3061 gcgaggtcga agagcgcgaa ctggaacccg accgtcgtgt tggactcgcc gcgtccgatc
     3121 acattcacgc gcacgttctc ccactcggat accttgacga agaggatcgc gtccacgttg
     3181 aggagcgaat cgatgccgcc caccgctgtc gtgtcgagcg cgccgtagtt cgaccacttg
     3241 tcgttgagcg cgaagagctt gtccgaggcg ttgtgcacct cgaggagacg ctccgtatcc
     3301 gtcggctgga ggaaggtggc ctgctcggcc ggaatctctt tcagtcggtc gtggaggatg
     3361 tcctccatga tcttgggggc gccgacctcg gccgagccgt tgatgaccgg catgatggcc
     3421 actcggatcg acttcgcggg cggcggatcg tccttcttct tggccccgag tgaggttgcg
     3481 aggaggaggg cgagcgcgga tgcggccgct atggagcgga tgagacgatg ggtcctcata
     3541 gtgctacctc gtgcggggaa ggtaccggcg tggggatcgg aggcgtcgat ctaaaaatgg
     3601 cgcgcccagg aggatttgaa cccccaacct tctgatccgt agtcagacac tctatccagt
     3661 tgagctatgg gcgcgcgcgc ctgggatgtt tcggtcgata tggcggagag gagaggattc
     3721 gaacctccgg taaggtatta gccctacaac ggtttagcaa accgccgcct tcagccactc
     3781 ggccacctct ccccgaaacg tcctagtcgg ttcagccgtc ggactatagc cgcggttgaa
     3841 ccgccggtca actgggagcg cccgaaccgg gccggccggc ttcccccaaa tccggcggct
     3901 tgcctccgga cagcgcccag gccggctcca catggatcgt gacctcgacc acctggggaa
     3961 gctggcgctg gatgtcctcc ttcactccgt gcgccagcgc atgactctcg cggaccgaca
     4021 tgtccggatc cacctccagg tgcatgtcgg ccacgtagta gatacccatc ttgcgcatca
     4081 ggagtttctc tactctgtaa accccccggc aggattcggc cacgcgctgg gtctcggcta
     4141 cgaggtcgta cggaggcgcc tggtccatga gctcgttgac ggccgggcgg aggaggcgcc
     4201 agccggtcac gacgatgatc ccgctcgcga agagggcggc ccagtcgtcg gcggcctcga
     4261 agccgcgccc cccgatcagg gccaccgcga tcccgatggc cgcggccccc gacgtgatgg
     4321 cgtcgctccg gtgatgccac gcatcggtgc gcacggtgtg gctcccggtc tcctggctga
     4381 cgcgtgaggt gaagcggaag aggagctcct tcacgacgac caccccgatg acgacgacga
     4441 gggtgaaggg ggccgggctc atgtgcggaa tcacgatctg gtggatcgat tccgcggcga
     4501 tcccgatggc ggcgacgatg aggagcacgg cgacgagggc cgctgcgatg ggctcggcct
     4561 tgccgtggcc gtaggggtgc tccatgtcgg gaggctggga cgctacctgg agcccgcgcc
     4621 agacgatgat cgagccgacc gagtccccca acgactcgat ggcgtccgcg atcagcgcct
     4681 ggctgtggcc gaggagtccc gccgcaagct tccccacgcc gaggaggaaa ttgaggacga
     4741 ggccgatcgc gctcgaccgc atgccgcggt cgtagcgctc ggagtggatc tcgtggcggt
     4801 ggcgaatggc catccggtca gggctcggtg gacgcctggg gttccggctc ggacgacccg
     4861 gaagcgaagg aggcgccgag gctgagatcg gacggcacgc cgttcgggaa attccgatgg
     4921 atcgccgcct cgatcacctg ccgtcgattc cccggatcgg gatgcgagct catgaactcc
     4981 ggctggctcc ctcctccgga cgcctgctcg aggatctgca tcagcacgat catcgcgcgc
     5041 ggatcgtagc ctgctcccgc catcatgcgc acccccagcg tgtccgcctg ggtctcgtcc
     5101 tgccggccgt agcggagctg catcatctgc gccaccagcg cggccacctg cgccgagtgc
     5161 tgcccggagt cgtagtccga cgagccggcg atccccgccg cgcccacgag cccctgcgcc
     5221 agctcgctct tcgagatctg ctccgcggag tgcctggcca ccacgtgccc gacctcgtgc
     5281 gcgagcacgc cggcgagctg ggcctcgttc tccagccgcg cgaggagccc gtgggtgatg
     5341 aagatttgtc ctcccggcag agcgaaggcg ttcacggttt gcggatccgc gagcgcgtgg
     5401 aactggaact ggtagggagt gctcgacgct cccccctgcg cgaccacgcg ctgccccact
     5461 tgctccacga cgcggcggac cgtggcgtca tcgtcctcgc cccccatctc ctgggccatt
     5521 tgcggcgcca tctggagccc gagcgcgatc tcctgctcgg gatccatgga gatgtgctgc
     5581 acttcacccg tgaacgggtt ctcctgccgc agggagtaat agctgatcag cgagatgacc
     5641 gccacgatcg cggcgatcag caatcgtcct cgcctcatgg ttccctccat cggcccggcc
     5701 ggctgcccgg gccagagtgt aggttggcga ggggtgtgag ggagcaagaa aagggccggg
     5761 gccccgtcag gcttggcgaa gaagtggggc gggtcgttcg tggggcggag cgacggcgct
     5821 ggcccttaga taggagacgg cgaggtccgt ggcgatcgag accgcgaggc gatcgttgct
     5881 ccagtacccg acgtgggaga gcggcgtcca gccggtccag agcgagcccg cactcaccgg
     5941 acggtcctcg gtgaccgcgc ggcggtagcc cgagttcagc tcgcgcaggg ggtatccgat
     6001 cacgtcgtcc ggatcgtaga cgttgatcca gcgcgcggcg agatcggggt gatggcgtac
     6061 gaggcgtctc ggcgggaacg cgatcggctg gccgaacttc ggatagcgca ggctccagag
     6121 cgcgatcggg cttccgagcg tgtagaggag cgcgagcgtc tcgccgcgct cgatgggcgt
     6181 gcgcccgacc gccgagcgaa cctcggcaca cgcgaacgat ttccgcttct tcgcgaggtc
     6241 gtagacgtag ttgctcgcga tcacggtgcc gaggctgtgg gcgatgatgc agagcggcgc
     6301 tcgggggccg gcccgctcgg ccaggcgctt gagggagcac gccacctcga ggtgcaccgc
     6361 atcgtaggcg gaccggtccg acggagcggg ctggtacgcg atggcgtccg ccgcgaaatc
     6421 gaccatgaag tggcgaagcc tcgcccaccc gagcttgcct gcctcctcga gccgctcgta
     6481 cagcaggtcc tcgcggttct ggagcgccgc gctccagttg atctcctcga agacgagcgc
     6541 gggagcgacc gcgtcagccg tgcgctcctc gaacgcgctt tggatcgagc gctgcatcgg
     6601 gaccgagtat ccaggctcgg tccggccgat accgtggatg aaggcgaccg cgatgggatg
     6661 ggccataccc ggtctatcgg acggagagcg ggatcgcttg agccgcccac gcgggaatcg
     6721 tggccgcgca gggccgtgga ttgtgaaaga attgggagat ggacgaggta acggaaagcc
     6781 ggccccgggc ggtggaagag ccccccaggg gctttctcga gagctcgaag atgcgccgtc
     6841 gcgcggcccg cgtgatcgcg gtcctggtgg atgctcttca gatcgtcgtg ttccccgcct
     6901 ttttccccgg catcgtctcg ccgttcgaca atgcgctcga tgtcctgacg gggatcgtca
     6961 tgacgttcct cctcggcttc cacgtcgcgt tcctgcccac gttcattgcc gagcttgtgc
     7021 ccttcgtcga tctgttcccc agctggacgc tcgccgtgct cttcgtcacg cggaagaagc
     7081 cgcttaaaga gtgaacaacg tcctggttcg cccagcccgg atcgaggacg cggtggccat
     7141 cgcgcgaatg gccaatgcgc tgaaccgcgc actcggagtc gacgtggact ccttcacccc
     7201 ggagcgcgtg cgcgaggatg cgttcggccc tatgcctctg gtctcgatcc tggtgggcga
     7261 ggtggacggc ggggccgcag gctacgcgtt ctaccatacc ggctacgact ccgacgtcgc
     7321 ggctcggaac ctcgtggtcg tggatctcta tgtcgaagag gaggcgcggg gccgcgggct
     7381 gggacaggcg ctcatggccg ggctcgcgcg ggaagcgctt cgattgggcg cgcgctcgat
     7441 gacgggaggt gttctcgatt cgaacgtgaa ggcgcttgcc ttctatcgaa ggctaggcgc
     7501 gaatcaggac ggcgtgcggt tcgtgacgtt ccctgagggt gcgctccgcg ccctggccga
     7561 ggggacgggc tagtcgcagg tcgtcgcgag accgatcacg cgggcgtccc gccgaactcg
     7621 cgcacgcgct tggcgagatc gaggggaagg atgcgctctt cggatgcttc gatctcctcc
     7681 gcggaccacc accgattctc gagaattcgc tcccgccggt gtatggcttc cacctcgggc
     7741 ccgaaggtgc ccgggtggcc ggtcacgcgg aagaacgcct cggtctgcga cacgtccttg
     7801 tccgcgaact cgaactccgt gaagctggtc cagagatagg tcagctcgac gtccgaggcg
     7861 cccagctcct ccgcggcctc gcgccgcgcc gcctgctctg gggattcacc ctcctcgatt
     7921 cccccacctg gcgtcgccca gaacttacgg cccaggccgt ccgcatgcag gaagaggaga
     7981 atacgtcccc gggagtcgaa aagggcgagc cgcgcggtcg tccgcgtctc gcggcgcctc
     8041 gccgtcaccg gctcctccgg atcgccgccc ccgcgatgcg ccactgtcgc tgctcggccg
     8101 tcgcgccatc cacgacggcg cggcggagcg tgtacgttcc cctatacgac tgggtgcgtt
     8161 tcccttcgta cgccaggagc cgcaccggga ctgtgatgta tcgcgagccg gccgcggcct
     8221 ccatgtcgga aggcgctccg atatcggcac gcaccgaatc ggtttgcgcg aagccgacgg
     8281 tgaaggactc cacggtctgc ccgctccgct cgccgcgccc cgaccagagg acatacgcat
     8341 cggagtaatg ccgcgcgttg atcgcgtcgt agtaggcgcg gatcactccc gccgcttcat
     8401 cgttggaagg ctcgcggagg gtgtcgaccg gcgcttcgcg tgagctcgac tccggtggcc
     8461 gtcgcgtggc cgtaccggcc ccgggctggg aacggccaca tcccaggacc acgaggagga
     8521 gtccgcacca cagccaggtc atcggaagcg ggggccgaag cataagatga tgatcgcgag
     8581 aaagaagaga agagtgacca gggccatcgt cgtcagcccg gccgccagga tcccccgcgc
     8641 gcgcttcggt cgatcgtgcg tgaattcgaa cacgatcgcg ggtgcgagat agagccattg
     8701 ccacaagcag aaggtcgcgg cggccggggg cagctgccgc aatgcggcgg ccaccaacag
     8761 aaccgggatc ccgacgatga ggtgaagctt catcccgaac cagaacccgg accagcgccg
     8821 attcttacgg tcttcgggcc ccataccttc gggcacaggc acgccgtcga ccacctcgtg
     8881 ttccatcagc tcactcccgc cagcgccagg gaccggagcg gctccaggcg agtcgactcc
     8941 aggccgtcgc gatagaacag gttgtagttg tcgaacggga tcagccgtcc gtccggatgg
     9001 acgatgtgca cgcagctctt cttgacgctt cgcacatcga acgaatgggc gtccaggaac
     9061 tgcatgatga tcacgcggaa cacgtcgcgg tacgtgatcg actccggtgc cgccacccgg
     9121 ggaagacagc agaggagatc gcgcaggctc cgcgccgagg aatcgggcga gtggttcgtg
     9181 gaaaagaggc ggaacacttc cgcctgaagg gtcctgtcct gctcgtagat gatcgtgttt
     9241 cgcccacctt cgatgagcac ctgcggatcg atgagccctg tcagcggaat gagcccttga
     9301 ttcgacttga gcgcgtacgc catggcgaga cagtccgggt ggcacggtac ggggatcaag
     9361 tcctcgggcc ggaacaggtt ggtctgctcg agaatccgac gcctcacttc ggtcagggtg
     9421 agccggttgt gctcggggtc gaacccctcg aggcgccccg ccgcttgcac aggctggaag
     9481 gtgacgccgc ggacgcatcg ctgcgccgcg gcgaattcga ggatggcgcc cagctctcca
     9541 tcattcacgc cacgcttcac ggtcacgacc agggtcgtcg agaggtcgag atcgttcagc
     9601 accgagagag cgccctcccg gatcgacctg agatccgcgc cccggagatc ctggagcgcc
     9661 gcccgctcga acgaatcgaa ctggaggtag agctcgaatc ccggcatgta ggccgcgaga
     9721 cgctccgcga atcccggctc ccgcgcgatc cggatcccgt tggtgttcac catgaggtgc
     9781 cggatcggcc gtcgtctcgc ggcatcgaga atggcgaaga attccgggtg ggtggtcggc
     9841 tccccacccg agatctggac cacgtcgggc tcccgctcgt tcgcgaccac ggcgtcgagc
     9901 atccgctcga tctcggccaa tgaacggtgg ccgggacggt gcggaccgct tgacgcatag
     9961 cagatcgggc aggtcaggtt gcagtggtcg gtcacctcga ccagcgccag acaggagtgc
    10021 tgctcgtgat cgggacagag gccgcagtca tagggacatc cgtaccggat gggtgtgttg
    10081 tagcggttcg gctgctccgg aggtttgagg aagatctccc ggcagcgttt gtaatagtcg
    10141 atgtcgtccg cgatgaggga gcactcccag ccgtgctcgg ggcagcgctt cttgaggtag
    10201 acctttccgt cctcgaagag gaccttccct tccactttgc gaaagcatcg cgagcaaatc
    10261 gagatcgcga tgtcgtagaa gagatacggg cggaccttat cggccatggg cggtctccgc
    10321 caggggtggg atcggatcgg gggtggcacg tgcgccggct acccgctccg ctcgggaccg
    10381 ctgggccacc cagaccaagt acgcgatgac gatcgtcccc acgatctggg tcgccgtgag
    10441 ccccagatac ggccgcggct ccggcttcac gaaatcgagc gctacacgga acgcgagata
    10501 gacgatcagg aatcctcgga agatcgaccc gtccatgacg cccgcgcgca gtcgccgtgc
    10561 cgcccagatc gcgatcggaa tcagcacg
//
LOCUS       DASTKC010000229         7818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_902732, whole genome shotgun sequence.
ACCESSION   DASTKC010000229 DASTKC010000000
VERSION     DASTKC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902732"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2489
                     /locus_tag="VFR25_09610"
     CDS             <1..2489
                     /locus_tag="VFR25_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941341.1"
                     /translation="GSAPNSRQVERLRTVYQLTDAVSRASAIGEVYEAALRGLERAVR
                     ADRASILLFDDDGVMRFKAWHGLSDAYRRAVEGHSPWSRDAVDAQPLGVPDVARDEAL
                     AAYRPLFESEGIRALAFIPLLSRGRLIGKFMVYFDHEHALDLEEFELARAIAGHVAFA
                     VERIRGEAKLALYREIFAHSSDAIAIIGADGRYLEQNVAHQELIGYADAEILGKTPEV
                     HLGGERFHEVRTFLEAQGVYRRDVISTTKSGVQLDIELSAFTVRDASGAPVCHVGIKR
                     DISQRKRMEEALQFLAAASAALDRSLDYQATVESLAGLTVPYLADWVVVETVGPDGSL
                     RPAASIHVDLRKAELLVQLVALRAGSAAPSGPVQDMAALKPKRVSGPADLLAELAEGD
                     IQAAELLGRIGAASAMVVPLRARGRILGRMTLVSTRRIYQDRDLALADDLSRRGATAL
                     DNARLYGEVQESDRRKEEFLAVLAHELRNPMTPLLTCLELMRSPHAEADSIARWREIM
                     ERQVRNLARLVDDLLDVSRITRGKIELQKQSIDLATVVARAVETTRPLIQSRGISLGV
                     AIPPGLKLFADPLRVEQVLANLLNNAAKYTPEGGRVDIEGEGDGVGVTVRVRDNGVGI
                     APDLLPRLFDLFQQGGNASGGLGIGLTLVRTLVELHGGGVRAMSAGPGKGSEFEVRLP
                     IGEAPPVPRTGPPALDSKGEPRRQRTLIVDDNVDAAMLLAEALRQRGYVVHLAHDGKT
                     AVEEALRSRPDVILLDLGLPDLDGYEVASRLRAQAGLEQSAIIAISGYGQAQYLTRSA
                     EAGFSHHIVKPVDMAHLEALLTALQTPNRP"
     gene            2521..4083
                     /locus_tag="VFR25_09615"
     CDS             2521..4083
                     /locus_tag="VFR25_09615"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9941342.1"
                     /translation="MLLLDVARTSERVASVSKRTEKIAALAECLRRLDPVEAAIGVAY
                     LSGRLPQGRIGVGGAAIRHTAQGPHAVEPSLTLAQVDAALTRIGGLAGPGSSAARQEA
                     LASLLGLATDLESSFLIRLLVGELRQGALGGVMEEAVSKASGIPADSIRRAAMVAGSL
                     DPVAVQALTRGPSGLASFKVGLFRPLSPMLAQPADDLDESLKAMGEAALEFKLDGARV
                     QAHKAGDEVRVYSRRLHDVTDSVPDLVEAVRGLHARELILDGEALALRPDGTPHSFQV
                     TMGRFATKLDVERSRRELPLTPFFFDLLRVDGDDLHDAPARERFRALREAAPSFAVSQ
                     RITSDPAEADAFFEEALQRGHEGVMMKDMEGRYEAGRRGAGWLKLKPAHTLDLVVLAA
                     EWGHGRRKGRLSNLHLGARDPQTGGFVMLGKTFKGMTDAMLEWQTKRFQSLALGTDGH
                     VVHVRPEQVVEIAFNDVQESPHYPGGLALRFARVKRYRDDKPADEADTIDTVRAIARA
                     EVRASRAKGSVR"
     gene            4080..5537
                     /locus_tag="VFR25_09620"
     CDS             4080..5537
                     /locus_tag="VFR25_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET9941343.1"
                     /translation="MSRGLFHGRTFDDFLFRPRMGRVGSRAGVRLGSKFSKGVALELP
                     VVSANMDSVTGSAMARTMALEGGIGIVHRALSIEDQAREVERVKRSHGWIVEDPIRLP
                     RTATLREARDTIRRHGVTGLLIEESRGSGILAGILSKRDMPWDPGREGDPVERHMTPF
                     DRLHVGRPGMSPDEAARILYEHRIEKLPVVDAERRIAGLITKSDILLSRERPDSTKDA
                     KGRLRVGAAVGARGDYLDRAEALLSAGADALVVDIAHGHSEVMAEALRALRERFPKAE
                     LVAGNVGTAEGALFLRDLGVAAVKVGIGPGRGCRTRLETAAGVPQLEAIYEVRLALGE
                     SVPIIADGGMRHDKDIVLALLCGASSVMLGSMLSGTDEAPGRVIEDPATHEKRKVYRG
                     MTSPQAVLATHYEAGEEEELERALETPAEGQELEVPYRGSVRDVLHRLRGHLRSAVSY
                     AGETSLGAARKVLLDDPEAFLVPLSAASIRESYER"
     gene            5602..6288
                     /locus_tag="VFR25_09625"
     CDS             5602..6288
                     /locus_tag="VFR25_09625"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9941344.1"
                     /translation="MDEAAAKSPGISLKQALGIPLNVLCFLFLMVLAWGSWAGFFAHP
                     ARVGVVVVHLLMMPVMTICTAGRSRGVRHEPDWKPFFPLLVFHSLFTAWVMPYMDARD
                     LFVLPGGDALRYAGLVILSLGALLRVWPMMTLGRRFASVVAIQEGHKLHTAGIYRWAR
                     HPSYVGILLMDVGFACVFRSSVAVLLLPLVFWMFHRRMDVEEALLVDAFGDEYKAYMG
                     RSARLLPGVY"
     gene            6288..6884
                     /locus_tag="VFR25_09630"
     CDS             6288..6884
                     /locus_tag="VFR25_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941345.1"
                     /translation="MRRSIVARAALVLALAGTGGCAAASQLAALRQVEFRFDHIGSPR
                     VADVPLERIPSYSDLSVADAARIGMAVASGVVPMDLTVHLTGRNPESNSVNARLVAMD
                     WAYLVDDKEAVSGRLTDPVSFAPGAAQDIPLRVRLDLMEVFDERGHDLVEVALALAGQ
                     GSASHRVTFRVAPTVDTPLGPIQYPAPLSFDISSNSGR"
     gene            7072..>7818
                     /locus_tag="VFR25_09635"
     CDS             7072..>7818
                     /locus_tag="VFR25_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9941346.1"
                     /translation="MALALTGCSSQKKTRSVRVPVRVAQAEEQSMPYALTSVGSVEAL
                     RTAAVGSQVGGVITRVAFREGDVVRKGQVLIQIDPRPFHAALEQAKATLQRDRAQAES
                     ARLDAERSLSLFQANVLSQAEWDQKRSASEALAATVRADSASVMNAALDLEYATIRSP
                     IAGRTGRLSVNVGDYVKTATSDALVTVIQPDPIRVRFTIPERDVPALQRYLHAQPKVE
                     ITSAAGGESTEGALIFVDNAVDQVSGTLLLK"
BASE COUNT         1159 a         2697 c         2746 g         1216 t
ORIGIN      
        1 tgggctccgc acccaacagc cggcaggtcg agcgcctccg caccgtctat cagctgacgg
       61 acgcggtgag ccgcgcgagc gcgatcggcg aggtctacga ggccgcgctc cgcgggctcg
      121 agcgggccgt ccgagccgat cgagcgtcga tcctcctctt cgatgacgac ggcgtcatgc
      181 gcttcaaggc gtggcacggt ctgtcggacg cctatcggcg ggccgtggag ggacactccc
      241 cctggtcgcg cgacgcggtc gacgcgcagc ccctcggcgt tccggacgtc gcgcgggacg
      301 aagcgctcgc cgcgtaccgt ccgctcttcg agtcggaagg gatccgcgcg ctcgcgttca
      361 tccctctcct ctcgcgcggc cggctgatcg ggaagttcat ggtctacttc gaccacgagc
      421 acgcgctcga cctcgaggag ttcgagctgg cgcgcgccat cgcggggcac gtcgcattcg
      481 cggtggagcg catccgcggc gaggcgaagc tcgcgctcta tcgcgagatc ttcgcccact
      541 cgagcgacgc gatcgccatc attggcgccg acggacggta cctggagcag aacgtggcgc
      601 atcaggagct gatcggctac gccgacgcgg aaatcctcgg caagaccccc gaggtccatc
      661 tgggggggga gcgcttccac gaggtgagga cgttcctgga agcgcagggc gtctaccgcc
      721 gggacgtcat cagcaccacc aaatcgggag tccagctcga catcgagctc tcggccttca
      781 ccgtgcgcga cgcctccggc gcgccggtct gccacgtcgg catcaagcgc gacatcagcc
      841 agcgaaagcg catggaggag gcgctccagt tcctcgccgc cgcgagcgcc gcgctcgacc
      901 gctcgctgga ctatcaggcg acggtcgaga gccttgccgg tctcaccgtc ccctacctcg
      961 ccgattgggt cgtggtcgag accgtgggtc cggacggctc gctccgtccc gcggccagca
     1021 tccacgtcga tctgagaaag gcggagctcc tggtccagct cgtcgcgctc cgcgcgggtt
     1081 cggcggcgcc gtccgggccg gttcaggaca tggctgcact caagccgaag cgcgtgagcg
     1141 gccccgccga tctcctggcg gagctggccg agggagacat ccaagccgca gagctcctcg
     1201 ggcggatcgg cgcagcctcc gcgatggtcg taccgctccg tgcgcgcggc cggattctgg
     1261 gccgcatgac gctcgtctcg acaaggcgca tctaccagga ccgcgatctc gcgctggcgg
     1321 acgacctctc acgccgcggg gccaccgcgc tcgacaacgc gcggctctac ggcgaggtgc
     1381 aggagtccga tcgacggaag gaggagttcc tcgcggttct cgcgcacgag ctccggaatc
     1441 ccatgacgcc gctcctgacc tgtctcgagc tgatgcgctc gccgcatgcg gaggccgatt
     1501 cgatcgcgcg gtggcgggag atcatggagc gtcaggtgcg gaatctcgcg cggctcgtgg
     1561 acgacctcct tgacgtgtcg cgcatcacgc gcgggaagat cgagctccag aagcagtcga
     1621 tcgacctcgc gaccgtggtg gcgcgcgccg tcgagaccac gcgccccttg atccagagcc
     1681 gcggcatctc gctcggcgtg gcgatcccgc cggggctcaa gctcttcgcc gacccgctcc
     1741 gggtcgagca ggtcctcgcc aacctcctca acaatgccgc caagtacacg cccgagggcg
     1801 gccgcgtgga catcgagggg gaaggggacg gtgtgggcgt cacggtccgc gtccgcgaca
     1861 acggcgtcgg catcgcgccc gatctcctgc cgcgcctctt cgatctgttc cagcagggag
     1921 ggaacgcgtc gggcgggctc ggcatcggac tcacgctcgt gcgcaccctg gtcgagctcc
     1981 acgggggcgg cgttcgcgcc atgagcgcgg ggcccgggaa gggaagcgag ttcgaggtcc
     2041 ggctcccgat cggggaagcg ccacccgtcc cgcgcacggg cccgcccgcg ctcgactcca
     2101 agggggagcc gaggcgccaa cgcacgctca tcgtcgatga caacgtggac gccgcgatgc
     2161 tcctcgccga ggcgttgcgc cagcggggat acgtggtcca tctcgcgcac gacgggaaga
     2221 cggccgtgga ggaggccctg cgcagcaggc ccgacgtgat cctcctcgac ctgggccttc
     2281 ccgatctcga cggctacgag gtcgcgagcc ggctgcgcgc gcaggccggg ttggagcaga
     2341 gcgcgatcat cgcgatctcg gggtacggtc aagcgcagta cctgacccgc tcggcggagg
     2401 ccggcttctc ccatcacatc gtgaagccgg tcgacatggc gcacctcgag gcgcttctca
     2461 ccgcgctcca gaccccgaat cgcccgtgag gctcgcgaga ccccagtccc gcatgcgttg
     2521 atgctgctcc tcgacgtggc acggacgtcc gaacgggtcg cctccgtatc gaagcgcacg
     2581 gagaagatcg cggcgctcgc ggagtgcctc cgccgcctcg atcccgttga ggccgcgatc
     2641 ggcgtggcgt atctctccgg gcgccttccg caggggcgga tcggagtcgg aggcgccgcc
     2701 atccgacaca cggcccaggg cccgcacgcg gtcgagccct cgctcacgct cgcgcaggtc
     2761 gatgccgcgc tcacgcggat cggcgggctt gccggtcccg gctcgtccgc ggcgcgccag
     2821 gaagcgctcg cctccctcct cgggcttgcg accgatctgg aatcgtcgtt cctgatccgg
     2881 ctcctcgtgg gcgagctcag gcaaggggcg ctcggtggcg tcatggagga ggcggtgtcg
     2941 aaggcttcgg ggatcccggc ggattcgatc cggcgcgcgg ccatggtggc ggggagtctc
     3001 gatcccgttg cggtccaggc gctcacgcga gggccgagtg gtctcgcctc gttcaaggtc
     3061 ggcctcttcc gtcctctctc gcccatgctc gcgcagcccg cggacgatct ggacgaatcc
     3121 ctgaaggcca tgggggaagc ggcgctcgag ttcaagctgg acggcgcgcg cgtccaggcg
     3181 cacaaggccg gggacgaggt gcgggtctac tcccgccggc tccacgacgt gaccgactcg
     3241 gtgcccgatc tggtcgaagc ggtccgaggg cttcacgcgc gcgagctcat cctggacggt
     3301 gaggcgctcg cgcttcgccc cgacgggacg ccgcactcgt tccaggtcac gatgggccgg
     3361 ttcgcgacca aactggacgt ggagcgctcg cggcgcgagc ttccgctcac gccgttcttc
     3421 ttcgatctcc tccgcgtcga cggggacgat ctccatgacg cgcccgcgcg ggagcgcttc
     3481 cgggcgctcc gcgaggccgc gccgtccttc gccgtgtccc agcggatcac gtccgatccc
     3541 gccgaagcgg acgccttctt cgaggaggcg ctccagcgcg gccacgaagg ggtcatgatg
     3601 aaggacatgg aggggcgcta cgaggcgggc cggcgagggg cgggctggct caagctgaag
     3661 cccgcgcaca cgctcgacct agtcgtgctc gcggcggagt gggggcacgg ccgccgcaag
     3721 gggcgcttga gcaacctcca cctgggagcc cgggatccac aaacgggggg attcgtgatg
     3781 ctcgggaaga ccttcaaggg aatgaccgac gcgatgctcg agtggcagac gaagcgcttc
     3841 cagtcgctcg cgctcggcac ggacggccac gtcgtccatg tccgtccgga gcaggtcgtc
     3901 gagatcgcgt tcaacgacgt gcaggagagc ccgcactacc ccggcggact cgcgctccgc
     3961 ttcgcgaggg tgaagcgcta tcgcgacgac aagccggccg acgaggccga cacgatcgac
     4021 accgtccgcg cgatcgcacg cgcggaggtt cgcgcgtccc gcgccaaagg aagcgtgcga
     4081 tgagccgcgg gctcttccac ggtcggacgt tcgacgactt cctcttccgc ccgcgcatgg
     4141 ggcgggtcgg atcacgcgcc ggcgtgcggc tgggctccaa gttctcgaag ggggtcgcgc
     4201 tcgagctccc ggtcgtctcg gccaacatgg acagcgtaac cggctccgcg atggcccgga
     4261 cgatggcgct cgaagggggg atcggcatcg tgcaccgcgc gctctcgatc gaggaccagg
     4321 cgcgcgaggt ggaacgcgtg aagcggagcc acggctggat cgtggaggat ccgatccggc
     4381 tcccgcgcac ggcgacgttg cgcgaagcgc gcgatacgat ccgccgccat ggcgtgaccg
     4441 gactcctcat cgaggaatcg cgcgggtcgg ggatcctggc cggcatcctc tcgaagcggg
     4501 acatgccctg ggatccgggg cgggaggggg atcccgtgga gcggcacatg accccgttcg
     4561 atcgtctcca cgtcggccgg ccgggcatga gccccgacga ggccgcgcgc attctctacg
     4621 agcacaggat cgagaagctg cccgtggtcg atgcggaacg gcgtatcgcc gggctcatca
     4681 cgaagagcga catcctcctc tcccgggagc gtcccgactc gacgaaggac gcgaaggggc
     4741 gcctccgggt cggagccgcc gtgggcgcgc ggggggacta tctggatcgc gcggaagccc
     4801 tcctctccgc gggggccgac gcgctcgtcg tcgacatcgc gcatgggcac tccgaggtga
     4861 tggcggaggc gctccgcgcg ctccgagagc ggttcccgaa ggccgagctc gtggcgggaa
     4921 acgtgggaac ggcggaagga gcgctcttcc tcagggacct cggcgtcgcc gccgtgaagg
     4981 tggggatcgg cccggggcgc ggatgccgga cgaggctcga gaccgcggcc ggcgtgcctc
     5041 aactcgaggc catctacgag gtccggctcg cgctcggcga gtccgttccg atcatcgccg
     5101 acggcgggat gcgccacgac aaggacatcg tgctcgcgct cctctgcggc gcgtcctcgg
     5161 tgatgctcgg cagcatgctc tccggaaccg acgaggctcc gggacgcgtg atcgaggatc
     5221 cggcgacgca cgagaagcga aaggtatatc gcggcatgac ctcgccgcag gcggtgctcg
     5281 cgacccacta cgaggcgggc gaggaggagg agctcgagcg tgcgctcgag actcccgcgg
     5341 aagggcaaga gctcgaggtg ccgtatcggg gaagcgtgcg cgacgtgctc cacagactcc
     5401 gcggccatct tcgctccgcc gtgagctacg cgggcgagac ctcgctcggc gccgcgcgga
     5461 aggtcctgct cgacgatccc gaggcgttcc tggtcccgct ctccgcggcg tcgattcggg
     5521 aatcctacga gcgatgacgg gcgtgcgcta gaatgcggcc acgcacccgg cgggtcgggg
     5581 cggaacggga agggaggtcg tgtggacgaa gccgcggcga agtcgccagg aatcagcctc
     5641 aagcaggccc tcggaattcc cctcaatgtt ctctgctttc tcttcctcat ggtgctcgcg
     5701 tggggaagct gggccgggtt cttcgcgcat ccggcccgcg tcggcgtggt cgtcgtgcat
     5761 ctcctcatga tgccggtcat gacgatctgc accgcgggac gaagccgtgg cgtgcgtcac
     5821 gagcccgact ggaagccctt cttcccgctc ctcgtcttcc actcgctctt caccgcgtgg
     5881 gtcatgccct acatggacgc gcgcgatctc ttcgtgctcc cgggcgggga cgcgctccgc
     5941 tatgcgggac tcgtgatcct ctcgctcggg gcgctgcttc gcgtctggcc gatgatgacg
     6001 ctcggacggc gattcgcgtc ggtcgtcgcg atccaggaag ggcacaagct ccacacggcg
     6061 ggcatctacc gctgggcgcg tcatccgtcc tacgtgggca tcctgctcat ggacgtcggc
     6121 ttcgcgtgcg tgttccggag cagcgtggcg gttctcctcc tgccgctcgt gttctggatg
     6181 ttccaccggc ggatggacgt ggaagaggcg ctcctggtcg acgcgttcgg cgacgagtac
     6241 aaggcctaca tgggccgctc ggcgcggctc ctgcccggcg tctattgatg cgccgatcga
     6301 tcgtcgcgcg cgccgccctc gtcctcgccc tggcggggac gggcggctgc gcggcggcga
     6361 gccagctggc ggcgctccgg caggtcgagt tccgctttga ccacatcgga tcgccgcgtg
     6421 tcgcggacgt tccgctcgag cggatcccct cctactcgga cctctcggtc gcggacgccg
     6481 cgcgcatcgg catggcggtg gcgtccggcg tcgtccctat ggatctgacg gtccacctca
     6541 cgggacggaa cccggaatcg aactccgtga acgcgcgcct cgtcgcgatg gactgggcct
     6601 acctggtcga tgacaaggaa gcggtctcgg gacgtctgac cgatccggtc tccttcgcgc
     6661 ccggggctgc gcaggacatt cccctgcgcg tacgactgga tctgatggaa gtcttcgacg
     6721 agcgagggca cgacctggtc gaggtcgcgc tcgccctagc cgggcaaggg agcgcgagcc
     6781 atagggtcac attccgggtc gcccccaccg tggatacgcc gctcggcccc atccagtatc
     6841 cggccccact ctccttcgat atctcctcga attcaggccg gtaatccatt ggaattgggc
     6901 tatttgaaag ctactcattt gccgcctttg cgttgcggcc aacgaaactc cctcgagcgc
     6961 tgtagaagat gtcgtatggc aacgagcgtc tgccggcgtc cggccggtcc tttttcccgc
     7021 gcaaggcccg gctcaagggc cgtccccccc gcactcacga tgctcctcac cgtggctctc
     7081 gccctgaccg gctgctcgtc ccagaagaag acccgatccg tccgcgtgcc cgtgagggtc
     7141 gcgcaggcgg aggagcagtc gatgccgtac gcgctgacct cggtcggatc ggtcgaagcg
     7201 cttcgcacag cggcggtcgg ctcgcaggtg ggcggcgtga tcacgcgcgt ggccttccgg
     7261 gaaggggacg tggtcaggaa ggggcaggtt ctgattcaga tcgatcctcg tccgtttcat
     7321 gccgcgctcg agcaggcgaa ggccacgctc cagcgtgatc gcgcgcaggc ggagagcgcg
     7381 cgtctggatg cggagcgcag cctgagcctc ttccaggcga acgtactctc gcaggcggaa
     7441 tgggaccaga agcgatccgc ctccgaggcg ctcgcggcga cggtgcgggc ggactccgcg
     7501 tcggtgatga acgccgccct cgacctcgaa tacgcgacca tccggtctcc catcgcggga
     7561 cgcacagggc ggctcagcgt gaacgtgggg gactacgtga agacggccac gagcgacgcg
     7621 ctcgtgaccg tgatccagcc cgatcccatc cgcgtgcgct tcacgatccc ggagcgcgac
     7681 gtgccggcgc tccagcgcta cctccacgcc caacccaagg tggagatcac gtccgccgcc
     7741 ggcggggagt cgactgaggg cgcgctcatc ttcgtcgaca acgcggtgga tcaggtgagc
     7801 gggacgctcc tcttgaag
//
LOCUS       DASTKC010000230         8485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_666082, whole genome shotgun sequence.
ACCESSION   DASTKC010000230 DASTKC010000000
VERSION     DASTKC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666082"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFR25_09640"
     CDS             <1..262
                     /locus_tag="VFR25_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HET9941347.1"
                     /translation="GCFSMALSGKLGAEGLTAESIRSTAAVTLDKVEGGFAVTAVHLD
                     VVARVPGADRAKFEKAAQDAKSGCPISKLLNARITMDARLED"
     gene            complement(288..2132)
                     /locus_tag="VFR25_09645"
     CDS             complement(288..2132)
                     /locus_tag="VFR25_09645"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET9941348.1"
                     /translation="MSAPERPVVVFDGDCGFCRQWIARWRQTLGDRAEFVPYQEAAPR
                     FPEIPEKAFREAVQLVEPDGRVSRGAEAVFRALSYAPRRRWPLRLYRRLPGFAPLTEA
                     CYRLVAGHRGLATRLTGWIWGRHVVPPGETLTTWIYLRLLALVYLVAFLSLWTQIIGL
                     AGTDGILPAQRYLDAAGAQYGVLGPWIAPTLTWISTSNAMLHALCAMGTLLALLLAVG
                     IAPILCLVGLFVLYLSATVPCQDFLWFQWDSLLLEAGFLAIFLAPWRWWSHPRRDPNP
                     SRAALWLSRWLLVRLSVSSALVKLLSGDRAWHDLSALRYHFETQPLPTWTAWYMHHLP
                     AGILSAMTVGVFVVEGATPFLAFGPRRVRFTAAAAIAFLQVLILLTGNYGFFNWLTLA
                     LCVLLLDDGVWPRRWRAAVEASTPRRGRWLSWMVTPAAVVLFALSWVPTLRGLGRPTA
                     WMGPVEILYEGVWPFRMVNHYGLFAVMTTRRPEIILEGSDDGTVWRAYEFRYKPGGLS
                     RRPEFVAPHQPRLDWQMWFAALGTPDSQPWFYPFCKRVLEGSPPVVGMFARDPFRGAP
                     PRFLRAVVYEYHFTTPAERKATGAWWTRTRLGLYGPVMMLSNGSLMLI"
     gene            complement(2146..4203)
                     /locus_tag="VFR25_09650"
     CDS             complement(2146..4203)
                     /locus_tag="VFR25_09650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9941349.1"
                     /translation="MTRLMTRTLRIAAPLALALSLGAVCPASSDAKTPKAKSSDPAFF
                     SGHPDAAAFKAMEDAELKAAQSALDKMLAVKGKRTIENTLVPYNEVQMHGENAAYGSS
                     LLEAVHPDSAFRADAEAQTQRATKFLDDLSLNRAVYDAIVQVDVSKADPTTRYFHEKT
                     LRDFRLSGVDKDEATRAQISTLLDELTKTGQDFDRNIRMDSRTIKVDGPAALAGLPDD
                     FIKSHPASEADGKITLSIEYPDLGPVMRYAKSEDTRRRLMHEAQNRAYPANMAVLDSL
                     IVKRDRLAHVLGYPTWADYITQDKMIENAANAQSFIDRLKDTTFKRAHEEYAVYLKRK
                     QEDDPKATQLNRWEASYYGRLIRMRDYDYDPGQARQYFPFDRVKQGVLDVTATMFGLT
                     YKRVTNAATWDPSVEVYEVYEGPKMLGRFFLDLHPRPGKYNHAAKFTIRQGVRGYQVP
                     EHALVCNFPGGKPGDPGLMEHSDVETFFHEFGHLIHAILGGQGQWEPVAGTATQRDFV
                     EAPSQMLEEWCWDAKVLQTFAKNEKGEPIPAELVQKMRKADAFGRALGISTQAFYSAV
                     SLSIYNRPPAQVNTDAIVTSLEPQFSPIPTMPDTHMQTAFGHLDGYSAYYYTYLWSLV
                     IAKDMFSRFNHDNLLDPTVAKRYRDMVLAPGGSKPAKALVHEFLERDYDFKAFDEWLA
                     GKG"
     gene            4577..6109
                     /locus_tag="VFR25_09655"
     CDS             4577..6109
                     /locus_tag="VFR25_09655"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9941350.1"
                     /translation="MDRRLANLGRLLWFDTLTGLNDDNTCAGCHSPTNGFGDTQSIAI
                     GIENNGVVGPDRTGPRNQRRAPSAMNTAFYPNLMWNSRFASLSGDPFDNRAGFVFPEP
                     EGTSLSYLPHLLDAQAFIPPTERNEVAGFTFVGGNHEMREEVLKRLNATPAYRDLFAA
                     SFADVKSGAPITFDHFGRALAEFEFTLTRANAPLDRYARGDLSSMTQGQKRGALLFFG
                     KAGCVRCHAVAGKSNEMFSDFQQHSIGMPQVCPDITNATFDGPKADEDFGLEQVTGRA
                     EDRYKFRTSPLRNLAVQAAYGHNGAFTTIEAVVRHHLDPHTSSREYSPSGLDADLRGA
                     TGPAEPVLNVVDPLLKSPAHLTDQEIGWLVEFLRDGLLDPRARYETLKHLIPRQVPSG
                     RATLTFEDPSPKPKRAVVAMASHDAPRVLDLSPAGLAAEGAVSFRFSLTERRSIEARV
                     YDATGRLVATLEQGSAYEPGSHELRWDGRTAEGSRAARGIYFLAVRAGEETARERIVK
                     LD"
     gene            complement(6119..6577)
                     /locus_tag="VFR25_09660"
     CDS             complement(6119..6577)
                     /locus_tag="VFR25_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9941351.1"
                     /translation="MKSNSIPVRPMTELDAEGVTALSTQLGYPSTAEQTLRRFRAISD
                     APDARVWVAEGPDGAIVGWIHLFGNRLLESDPDLEIGGLVVHEDARGRGVGRALVGAA
                     EVWARERGYSVVSVRSNVIRKDAHEFYKGLGYEPIKSQFKFRKKLSTEGG"
     gene            6623..7075
                     /locus_tag="VFR25_09665"
     CDS             6623..7075
                     /locus_tag="VFR25_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941352.1"
                     /translation="MLFGVRAVVCSRWLAASLLCGLALAGCSTDTSRLDPELLRRFQA
                     EGIVRREDNMVFRYTEGAGTRASSWEDRRGSIVVTHQTIFIHKNEKIGLQITPRTRKV
                     VEVERRGQRIRIRTGQGRAAEIWSFVPTDDPEGWARDLRAVLKASAPG"
     gene            7109..7723
                     /locus_tag="VFR25_09670"
     CDS             7109..7723
                     /locus_tag="VFR25_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HET9941353.1"
                     /translation="MIRLLPQETKFFDLFEAQAAKVHEAARALEDLARNYDQVQEKAR
                     HIKGLESQADQITHDIIDKLNRTFITPFDREDIHALASALDDVLDNIEGVSSRLALFE
                     ILQVTPEVIQLTSIIEQACASMVKAVGHMRDFTKINEYLIQINHLENEADVISRNATA
                     ALFRNAADFHALIKWKEIYGRLEATTDDCEDVANILEGIVVKNT"
     gene            7739..>8485
                     /locus_tag="VFR25_09675"
     CDS             7739..>8485
                     /locus_tag="VFR25_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET9941354.1"
                     /translation="MQDTFTAYIILIIVLALVFDFLNGFHDAANSIATVVSTRVLTPG
                     IAVIWAAFFNFVAVFVLGTAVAKMVGKGIVLPEAVNNHVLASALTGAIFWNLFTWWLG
                     LPTSSSHALIGGLVGAALAYKGPGSIIVPGLTKTAVFIVIAPVVGLLLGFFNMIAVSW
                     IFRRAHPGKVDHLFRKMQLVSAALYSLGHGGNDAQKTMGVIALLLFTTGQIDHFFIPQ
                     WVVIVCGIAIGLGTLTGGWRIVHTLGARVTK"
BASE COUNT         1418 a         2803 c         2875 g         1389 t
ORIGIN      
        1 gggctgcttc tccatggccc tctcgggcaa gctcggcgcc gaaggcctga ccgccgaatc
       61 catccgctcc acggccgcgg tcacgctcga caaggtagag ggcggcttcg ccgtcaccgc
      121 ggtccatctg gacgtggtcg cccgcgttcc gggcgccgat cgcgcgaagt tcgagaaagc
      181 cgcgcaggac gccaagtcgg gctgtcccat ctcgaagctc ctgaacgcgc ggatcacgat
      241 ggacgcgagg ctcgaggact gattcccgcc cgcgcgttcc gggcctctca gatcagcatc
      301 agactcccgt tcgagagcat catcaccggt ccatagaggc cgaggcgcgt ccgggtccac
      361 cacgctccgg tcgccttccg ttcggccggc gtcgtgaagt gatactcgta aacgaccgcc
      421 cggaggaacc ggggcggcgc tccgcggaaa ggatcgcgcg cgaacatccc cacgaccggc
      481 ggcgatccct cgagcacccg cttgcagaac gggtagaacc agggctgcga gtccggcgtg
      541 ccgagcgcgg cgaaccacat ctgccagtcg agccgcggct ggtgcggcgc cacgaactcg
      601 gggcgccggg acaggccgcc cggcttgtag cggaattcgt aggcccgcca caccgtcccg
      661 tcgtcgctcc cctccaggat gatctccggc cgccgcgtgg tcatgaccgc gaagaggccg
      721 tagtggttca ccatccggaa cggccacacc ccctcgtaga ggatctcgac cggtcccatc
      781 cacgccgtcg gccgccccag gccgcgaagc gtcggaaccc agctcagggc gaagaggacc
      841 accgcggcgg gcgtgaccat ccacgagagc caccgtcctc gacgaggcgt ggacgcctcc
      901 accgcggcgc gccatcgcct cggccagacg ccgtcgtcga ggagcagcac gcagagcgcg
      961 agcgtgagcc agttgaagaa tccatagttg cccgtgagaa ggatcaggac ctgtaagaat
     1021 gcaatggcgg cggctgccgt gaaccggacg cgccgcggcc cgaacgcgag gaaaggggtc
     1081 gcaccttcaa ccacgaacac gccgaccgtc atcgcgctca ggatccccgc cggcaggtgg
     1141 tgcatgtacc acgcggtcca cgtgggcaag ggctgggtct cgaagtgata gcggagcgcg
     1201 ctcaggtcgt gccacgcgcg gtcgccgctc aggagcttga ccagcgcgga cgagaccgag
     1261 agccggacca ggagccagcg gctcagccag agcgcggccc gcgacgggtt cggatcgcgg
     1321 cgcggatggg accaccagcg ccacggagcg aggaagatgg cgaggaagcc ggcctcgagg
     1381 agcaggctgt cccactggaa ccagaggaag tcctggcacg gcaccgtggc cgagagatag
     1441 aggacgaaga gcccgacgag gcagaggatc ggagcgatcc cgaccgcgag gagcagcgcg
     1501 agcagggttc ccatggcgca gagcgcgtgc agcatggcgt tgctggtgct gatccacgtg
     1561 agcgtgggtg cgatccacgg ccccagcacg ccatattgcg ctccagcggc gtcgagatag
     1621 cgctgcgcgg gaaggatgcc gtccgttccc gcgaggccga tgatctgggt ccagagcgac
     1681 aggaacgcga cgagatagac gagcgcgagg agccggaggt agatccaggt ggtcagggtc
     1741 tcgcccggcg ggacgacgtg gcgtccccag atccatccgg tcaaccgcgt ggccagtccg
     1801 cggtgccccg cgacgagccg gtagcacgcc tccgtgagag gagcgaatcc tgggaggcga
     1861 cggtagaggc ggagcggcca gcgccggcgc ggcgcgtagg acagcgcgcg gaagaccgct
     1921 tcggcgccgc gggacacgcg gccgtccggc tcgacgagtt ggacggcttc gcggaaggct
     1981 ttctcgggga tctcgggaaa gcggggcgcc gcttcctggt agggaacgaa ctccgcgcgg
     2041 tccccgagcg tctgccgcca gcgggcgatc cactggcggc agaatccgca gtcgccgtcg
     2101 aagacgacga ccgggcgctc gggcgcgctc acaacgatgc gatcatcatc cctttccggc
     2161 gagccactcg tcgaacgcct tgaagtcgta gtcgcgttcg aggaactcgt gcaccagcgc
     2221 cttcgcgggc ttcgacccgc cgggcgcgag caccatgtcc cggtaccgct tggccaccgt
     2281 gggatccaac aggttgtcgt ggttgaaccg gctgaacatg tccttcgcga tcacgagcga
     2341 ccataggtag gtgtagtagt aggccgagta gccgtcgaga tggccgaacg cggtctgcat
     2401 gtgggtgtcg ggcatcgtcg ggatcggtga gaactggggc tcgagcgagg tgacgatcgc
     2461 gtccgtgttc acctgcgccg gcggccggtt gtagatgctg agcgacacgg cggagtagaa
     2521 cgcctgggtc gagatcccga gcgcgcgccc gaacgcgtcg gccttccgca tcttctgcac
     2581 cagctccgcc gggatcggct ctcccttctc gttcttcgcg aaggtctgga gcaccttggc
     2641 gtcccagcac cactcctcga gcatctgcga cggggcctcg acgaagtcgc gctgtgtcgc
     2701 cgtcccggcg accggctccc actgtccttg gccgccgagg atggcgtgga tcaaatgccc
     2761 gaattcgtgg aagaaggtct ccacgtcgga gtgttccatg agcccgggat cgcccggctt
     2821 gccgccgggg aagttgcaga cgagtgcgtg ctccgggacc tggtagccgc gcacgccctg
     2881 gcgaatcgtg aactttgccg catggttgta tttccctggc ctcgggtgga ggtcgaggaa
     2941 gaaccgcccg agcatcttcg ggccctcata gacctcgtag acctcgacgc tcggatccca
     3001 cgtagccgcg ttggtcacgc gcttgtaggt gagcccgaac atggtcgcgg tgacgtcgag
     3061 aacgccctgc ttgacccgat cgaacgggaa gtactggcga gcctggcccg gatcgtagtc
     3121 gtaatcgcgc atccggatca gccggccgta gtaggaagcc tcccagcgat tgagctgcgt
     3181 cgccttggga tcgtcctcct gcttccgctt gaggtagacc gcgtactcct catgcgcgcg
     3241 cttgaaggtg gtgtccttca gacggtcgat gaagctctgc gcgttggccg cgttctcgat
     3301 catcttgtcc tgcgtgatgt agtccgccca ggtggggtat ccgaggacgt gcgccagacg
     3361 gtcgcgcttg acgatcagcg agtcgaggac ggccatgttc gcggggtagg cccggttctg
     3421 cgcctcgtgc atgagccgcc gcctcgtgtc ctcgctcttg gcgtaccgca tcacggggcc
     3481 gaggtcggga tactcgatgc tcagcgtgat cttgccgtcc gcctcggacg caggatgcga
     3541 cttgatgaag tcgtcgggga gtccggccag ggccgcgggg ccgtcgacct tgatggtgcg
     3601 cgaatccatg cggatgtttc ggtcgaagtc ctgaccggtc ttggtcagct cgtcgaggag
     3661 cgtcgagatc tgcgcccgcg tcgcctcgtc cttgtccacg ccggacaggc ggaagtcgcg
     3721 cagcgtcttc tcgtggaagt agcgcgtcgt cggatccgcc ttgctgacgt ccacctggac
     3781 gatggcgtcg tagaccgcgc ggttcaggct caggtcgtcg aggaacttcg tcgcccgctg
     3841 ggtctgggcc tcggcatcgg cccggaacgc cgagtcgggg tgcacggcct cgagaaggct
     3901 ggacccgtag gccgcgttct cgccgtgcat ctggacctcg ttgtagggga cgagcgtgtt
     3961 ctcgatcgtc cgcttgcctt tcacggcgag catcttgtcg agcgccgact gcgcggcctt
     4021 gagctcggcg tcctccatgg ctttgaacgc agccgcatcc gggtgcccgc tgaagaacgc
     4081 ggggtcggac gacttggcct tgggcgtctt ggcgtcggac gaagcgggac aaaccgcgcc
     4141 gagcgacagg gcgagtgcga gcggggctgc gatacggagc gtgcgggtca tgagtcgggt
     4201 catagcgcga tcaatgcctc ctcgaagggc ctgatggtga aatggcgttc gatgatgcca
     4261 tggggtcctg gaaccggcaa ggcgatcgaa aacgaagttg gcgggacgga gcgggttctc
     4321 taggcttcac gacgccgggc cgcaccatgg ggaggcgggt ggcccgggac catgccacca
     4381 cgagggggcg cctgtcttgg cccgtcgtcc cgaacgactc ttgctacctg ccctgtctgc
     4441 cctggctctg ggattcgggc tgggctttgc cccggccccg gccggggaga tcgatcgcga
     4501 cctcacgttg gttctccagc agcaaggatt tacggggaaa atcgaatcta ctttggagcg
     4561 ccgtctcggg aggccggtgg atcgccggct ggccaacctg ggacgccttc tctggttcga
     4621 taccctgacc ggcctgaacg atgacaacac ctgcgccggc tgccactcgc caaccaacgg
     4681 tttcggggac acccagtcga tcgcgatcgg gatcgagaac aacggggtcg tcgggccgga
     4741 ccgcacgggg ccgcgaaacc agaggcgggc cccgtcggct atgaacacgg ccttctatcc
     4801 gaacctcatg tggaactccc gcttcgcctc gctctccggc gatccgttcg acaaccgggc
     4861 ggggttcgta ttcccggagc cggaagggac gtccctctcg tacctgccgc acctcctcga
     4921 cgcccaggcc ttcattcctc cgaccgagcg gaacgaggtc gccgggttca cgttcgtggg
     4981 cggcaatcac gagatgcggg aggaagtgct gaagcggctc aacgccacgc cggcgtaccg
     5041 cgacctcttc gcggcgtcgt tcgcggacgt caagtcgggc gctccgatca cgttcgacca
     5101 cttcggaagg gcgctggccg aattcgagtt cacgctcacc cgggcgaacg cgccgctgga
     5161 tcgctacgcg cgtggcgatc tttcctccat gacgcaggga cagaagcgcg gcgcgctcct
     5221 cttcttcggg aaggccggat gcgtgaggtg ccacgcggtg gcggggaagt cgaacgagat
     5281 gttcagcgac ttccagcagc actcgatcgg catgccccaa gtctgtccgg acatcaccaa
     5341 cgcgacgttc gacggtccga aagccgacga ggacttcgga ctggagcagg tcacgggacg
     5401 agccgaggac cggtacaagt tccggacgtc gccgcttcgc aatctcgccg tgcaggcagc
     5461 ctacggacac aacggcgcct tcacgaccat cgaggcggtc gttcggcatc acctcgaccc
     5521 gcatacgtcc agccgggagt actcgccctc ggggctcgac gccgacctgc gcggcgcgac
     5581 ggggccggcc gagcctgtgc tgaacgtcgt ggatccactc ttgaagtcgc cggcgcatct
     5641 cacggaccag gagatcggct ggctggtcga gttcctgcga gacggcctcc tggacccgcg
     5701 ggcgcgctac gagaccctca agcacctcat tccgcggcaa gtgccgagcg gccgcgccac
     5761 gctcacgttc gaggatccct cgcccaagcc gaagcgggcc gtcgtcgcga tggcgtcgca
     5821 cgacgcaccc cgcgtgctcg atctgagccc tgccggcctc gcggcggaag gagcggtgtc
     5881 cttccgcttc tcgttgacgg agcgccgatc catcgaggcg cgcgtgtacg acgcgaccgg
     5941 acggctcgtg gcgacgctcg agcagggatc cgcctatgag cccggctcgc acgagctccg
     6001 ctgggacggc cggacggcag aggggagtcg cgccgcccgc gggatctact tcctcgcggt
     6061 gcgcgcagga gaagagacgg ctcgcgagcg cattgtgaag ctggattgag cgccgggctc
     6121 agccgccttc ggtggagagc ttcttccgga acttgaactg ggacttgatc ggctcgtagc
     6181 cgaggccctt gtagaactcg tgggcatcct tccggatcac gttggagcgg accgagacga
     6241 ccgaatagcc gcgttcgcgg gcccagacct ccgcggcgcc cacgagcgcc cgccccacgc
     6301 ccctgccgcg cgcgtcctcg tgcacgacga gcccgccgat ctcgagatcg gggtccgact
     6361 cgaggaggcg gtttccgaac aggtggatcc agcccacgat cgcgccgtcc ggtccctccg
     6421 ccacccagac gcgcgcatcg ggggcgtcgc tgatggcgcg gaagcgccgg agcgtctgct
     6481 ccgccgtcga gggatagccg agctgcgtgg agagcgccgt gacgccctcg gcgtccagct
     6541 ccgtcatcgg tcgcactgga attgaattcg atttcatggc gtaatgcgcg ccatggtggc
     6601 cgcccggggg gaaaaaggca aggtgctctt cggcgtccgt gcggtcgtgt gctcgaggtg
     6661 gctcgcggcg tctctcctat gcggcctcgc gctcgccggt tgctccacag acacgagccg
     6721 gctggatccc gagctcctgc gccgcttcca ggccgagggg atcgtccggc gcgaagacaa
     6781 catggtgttc cgctacacgg agggcgccgg cacgcgcgcc tcgagctggg aggatcgccg
     6841 cggctccatc gtggtcacgc accagacgat cttcatccac aagaacgaaa agatcggcct
     6901 ccagatcacg ccacgcaccc ggaaggtggt ggaggtggaa cgccggggcc agcggatccg
     6961 gattcggacg gggcagggcc gggcggctga aatctggtcg ttcgttccca ccgacgaccc
     7021 ggaagggtgg gcccgggacc tgcgagccgt tctcaaggcc tccgctcccg ggtagacacc
     7081 gcgccacccg gtctgtaaca ctgttctcat gattcgactg ctcccacagg agacgaagtt
     7141 cttcgatctc ttcgaagcgc aggccgcgaa ggtccacgag gccgcgcgcg cgctcgagga
     7201 cctggcccga aactacgacc aggtgcagga gaaggcccgg cacatcaagg ggctcgagag
     7261 ccaagccgac cagatcaccc acgacatcat cgacaagctg aaccgcacgt tcatcacgcc
     7321 gttcgaccgc gaggacatcc acgcgctcgc gagcgcgctc gacgacgtgc tcgacaacat
     7381 cgagggagtg tcgagccggc ttgcgctctt cgaaatcctt caagtgacgc ccgaggtcat
     7441 ccagctcacg tcgatcatcg agcaggcgtg cgcatcgatg gtgaaggcgg tcggccacat
     7501 gcgggatttc acgaagatca acgagtacct cattcagatc aatcatctgg agaacgaggc
     7561 cgacgtcatt tcgaggaacg ccacggcggc cctcttccgc aacgccgccg atttccacgc
     7621 gctgatcaag tggaaggaga tctacggccg cctcgaggcc acgaccgacg actgcgagga
     7681 tgtggccaac atcctggagg ggatcgtcgt caagaacacc tgacgacgcc ggcccgggtt
     7741 gcaggacacg ttcaccgcgt acatcatcct catcatcgtc ctcgcgctcg tcttcgattt
     7801 cctgaacggc ttccacgacg ccgccaactc catcgccacc gtcgtctcca cccgcgtcct
     7861 gaccccgggc atcgccgtga tctgggcggc cttcttcaat ttcgtcgccg tgttcgtcct
     7921 cgggactgcg gtggcgaaga tggtggggaa ggggatcgtt ctgcccgagg ccgtgaacaa
     7981 tcacgtgctc gcctcggcgc tgacaggcgc gatcttctgg aatctcttca cctggtggct
     8041 cggccttccg acgtcgtcct cgcacgcctt gatcggcggc ctggtcgggg cggcgctcgc
     8101 gtacaagggc ccgggctcga tcatcgtgcc ggggctcacg aagacggcgg tcttcatcgt
     8161 gatcgctccc gtcgtcgggc tcttgctcgg cttcttcaat atgatcgccg tctcctggat
     8221 cttccgacgc gcacatccgg gcaaggtgga tcacctcttc cggaagatgc agctcgtctc
     8281 ggccgcgctc tattcgctcg ggcacggcgg aaacgacgcg cagaagacca tgggggtgat
     8341 cgcgctcctc ctcttcacga cggggcagat cgatcacttc ttcatcccgc aatgggtggt
     8401 gatcgtctgc ggcatcgcga tcgggctcgg cacgctcaca ggcgggtggc ggatcgtgca
     8461 cacgctcggc gcgcgggtca cgaag
//
LOCUS       DASTKC010000231         9210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1612994, whole genome shotgun sequence.
ACCESSION   DASTKC010000231 DASTKC010000000
VERSION     DASTKC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612994"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VFR25_09680"
     CDS             <1..1299
                     /locus_tag="VFR25_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9941355.1"
                     /translation="HEHADRYNAFATVTPDLALEQAREAEREIGRGRYKGPLHGIPYG
                     AKDLLATAGIKTTWGAAPTKDQMFDEDATVIRKLREAGAVLLGKLSMVEFAGCLGYRE
                     AGASLQGPGRNPWDRERWTGGSSSGTGASVSAGFVAFGIGTETWGSILCPSAFCGVTG
                     LRPTYGRVSRAGGMVGSYTLDKIGPMARSAADCRLILRAIAGSDPRDLTSSDEPVEID
                     RRRPNFGRLRAALIPSDFAKTPGAEREVGARFDGAVEDLRRLGLKIETAALPEIPASE
                     LTTTILYAEAISAFEPFFEDGSVRKLHDPYAPYQREVTAALTGADYIKASRMRTVLQE
                     RMAEFFSTYDVIVATNFLSVAPSIHEDFTKTLPYPDPVGAVGNGCGLPAIALPMGPGK
                     DGLPTSFQIMGSPFEEGLLLDLGEAYQGATRHHLARPPVA"
     gene            complement(1611..2264)
                     /locus_tag="VFR25_09685"
     CDS             complement(1611..2264)
                     /locus_tag="VFR25_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9941356.1"
                     /translation="MGCTSSGADRGGTLGDGTALAGDDDGSEPKLRTTIFLLAQIRDG
                     NQAARTKLWNRYFKPLGRLVRGHYIPHRVRALHDVDDIVMRVIEQVDPKLLDFEYRRE
                     GALMDYLRTVTRNLIISLSRVREPEREEMPTNIATDEPTPHERAVAMDQREAFEAALK
                     ILDERQRQAVILRFEYGFKYEEIAEEVDLASGNAARMMIQRAMLKMVEHMRGHRDFD"
     gene            complement(2400..2744)
                     /locus_tag="VFR25_09690"
     CDS             complement(2400..2744)
                     /locus_tag="VFR25_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HET9941357.1"
                     /translation="MGERTRKERCMPAKKRASKPATKKKTAARTKRKPNAAFMKPLTP
                     TGPLSEVVGAKAIPRTEVTKKLWAYIKKNKLQDKTKRTMINADDKLRAVFGGKRQVSM
                     FEMTKLVSKHLK"
     gene            complement(2840..2916)
                     /locus_tag="VFR25_09695"
     tRNA            complement(2840..2916)
                     /locus_tag="VFR25_09695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2880..2882),aa:Gly,seq:gcc)
     gene            complement(2995..4584)
                     /locus_tag="VFR25_09700"
     CDS             complement(2995..4584)
                     /locus_tag="VFR25_09700"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET9941358.1"
                     /translation="MAQRFRAAILAPECTPFAKVGGLGDVAGALSRAVADLGCSVAVF
                     LPRYADLELPPGSDLELVAKIDVPVSGEDQPGLVYKLRNGPYPTHLHHFFLANETYFG
                     RHGIYGDPSTSEEFADNAARFTFLSRGSLEAMRATAYVPDVIHANDHQTALVTAYMKT
                     LYANDPFFQMTATVLSIHNLGYQGVYAPEAMAIAGFPERFFYPLSPFEFWGKMNVLKA
                     GIQFADRLTTVSERYAEEIQSSEEFGFGLQGVLNARHADLTGILNGIDVKEWNPRTDR
                     AIAARYDAESLDGKGVCKAALLEELGLEPGDGAPLVGIIARLVEQKGFDLVEQAAERL
                     FERPAAWAVLGRGSARFETLFREWSRRKPRQVAFRAEFNDPLAHRIEAGADFFLMPSR
                     YEPCGLNQMMSMRYGTVPIVRETGGLADTVTPFGPVDLAQGTGILFRPYTPQALLDAV
                     DRALALYQDPVSMARARRNGMSQDFSWERSARKYVQLYRAANGAKRMGSGFGRWLEAV
                     ERGEGAEAEAPGQGPGWEPPA"
     gene            complement(4584..5588)
                     /gene="galT"
                     /locus_tag="VFR25_09705"
     CDS             complement(4584..5588)
                     /gene="galT"
                     /locus_tag="VFR25_09705"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9941359.1"
                     /translation="MRELRKDPVVGRWIILSSERRLRPSDFQETDPPPSNGGRESCPF
                     CPGHEGHTPPEILAFRDHGAPNEPGWTTRVFPNKFPALRVEETLERRGEGLYDRMSGV
                     GAHEVIVESPEHEKDLSELPVEQIERVLEAYKERILDLKKDTRLQYVLVFKNAGRKAG
                     ASLEHGHSQLIATPIVPRVVMEELEGSRAHWEAKERCVFCDMIDHERKDGRRVVLETE
                     RFLAIEPYAPRFPFETWILPRRHRAGYEDADPQDLQAFASILKETLGRIRRALDHPHY
                     NFVLHTAPWTTAERDSYHWHLEIMPKRTPAAGFEWGSGFHINPTAPEEAADYLRGLRA
                     "
     gene            complement(5604..6065)
                     /locus_tag="VFR25_09710"
     CDS             complement(5604..6065)
                     /locus_tag="VFR25_09710"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET9941360.1"
                     /translation="MITPDELASIPDPVPGAGAAPIEERPLNPDAKPLPVAPARTSAA
                     PSPTTSLQAEPPPSTSSVPPSGTAPEASAPSTAVWRVQVFASPDRAQAERVAREAAQK
                     LGAPYVLAKDGDLVKVRLGSFVREEDAQALKERAVQSGYSGAFRVLDKGGS"
     gene            complement(6232..7296)
                     /locus_tag="VFR25_09715"
     CDS             complement(6232..7296)
                     /locus_tag="VFR25_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HET9941361.1"
                     /translation="MRLYPVILAGGRGERFWPLSRRARPKQLLPLLSERSMLADTLAR
                     LDPLAPASSAWILTARDLAPAVRAAVPDVPELQVVGEPTGKNTAPAIALASWWLHGSG
                     ADAVAVVLPSDHRIEPADAFRNDLEEAAQTALERSAIVVIGIPASRPETGYGYIEMGE
                     PVAKGSRARRVAAFREKPDRETAARYVASGHLWNAGMFVFTPEVMLKEIRAHAPEIAS
                     ALEGMPRPGEPGAERALERYYAAVPSISIDYAVMERTRNALVLPARFGWDDLGSWAAL
                     GGDARDAAGNAVRGRALLEDVTGSVVFAEDGIVAALGVRDLVIVRTGDVTLVCPRERA
                     QDVRALVERLRREDDGSALL"
     gene            complement(7312..7920)
                     /locus_tag="VFR25_09720"
     CDS             complement(7312..7920)
                     /locus_tag="VFR25_09720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9941362.1"
                     /translation="MSLYRRGGKRVLDVALTLLLAPLALPVILVAAVAIKLDSPGPVF
                     HRAIRVGRGGRKFTFLKLRSMQIGAEELRGLLLHLNQAEGPAFKLHNDPRVTRVGKII
                     RKASLDELPQLWHVLVGDMSLVGPRPPFPEEVERYEPWMLRRLSVRPGLTCLWQIRGR
                     SDLSFDEWMRLDIEYVERHSLALDLKILVLTIPAVLSARGAY"
     gene            complement(7994..8200)
                     /locus_tag="VFR25_09725"
     CDS             complement(7994..8200)
                     /locus_tag="VFR25_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9941363.1"
                     /translation="MDSRICSGFDVRGRLVRTLKDESDAVAGYHDVRIDGIDASGGRL
                     SSGVYYVRIRAGADEERKAITILK"
     gene            complement(8572..>9210)
                     /locus_tag="VFR25_09730"
     CDS             complement(8572..>9210)
                     /locus_tag="VFR25_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9941364.1"
                     /translation="FVAGGNKQINLQSGKPAWCAQIEAQDYDVTDIDLSSIVLRSAGT
                     GSVQEIRALSDKTSVGGDRDGDGLSEITACFRKDDLRQLFSNVTGTQQIQVTLTGNLL
                     SGGPICAATLTVSVKGGGGAIAASITPNPLNPSAVLTFTTQERGPVNVQLFDVHGRLL
                     RTLQDESDAAAGYHDVRIDGTDASGARLSSGIYYLRIRAGTAEERKAITILK"
BASE COUNT         1441 a         3104 c         3177 g         1488 t
ORIGIN      
        1 cacgagcacg ccgaccggta caacgcgttc gcgacggtca cgcccgacct cgccctcgag
       61 caggcgcgcg aggcggagcg cgaaatcggc cggggccgat acaagggtcc gctccacggc
      121 attccctacg gcgcgaagga cctcctcgcg accgcgggga tcaagacgac gtggggcgcc
      181 gcgcccacga aggatcagat gttcgacgag gacgccaccg tgatccggaa attgcgcgag
      241 gccggcgccg tcctcctcgg aaagctctcg atggtggagt tcgcggggtg cctcggctac
      301 cgggaggcgg gcgcgtcgct ccagggcccg ggccgaaacc cgtgggatcg ggagcggtgg
      361 accggcggat cctcgtcggg cacgggcgcc tcggtctcgg cgggcttcgt ggcgttcggg
      421 atcggcaccg agacgtgggg atcgatcctc tgcccgtccg ccttctgcgg ggtcacgggg
      481 ctccgtccca cctacgggcg cgtgagccgc gccggaggca tggtgggctc gtacacgctc
      541 gacaagatcg gacccatggc ccggagcgcg gccgattgcc ggctcattct ccgcgcgatc
      601 gccggttccg atcccaggga tctcacgtcg tccgacgagc ccgtcgagat cgatcgccgg
      661 cgtcccaact tcggccggct ccgcgcggcg ctcatcccga gcgacttcgc gaagactccc
      721 ggggcggagc gggaggtggg cgcgcgcttc gacggtgcgg tggaggacct gcgtcgtctg
      781 gggctcaaga tcgaaaccgc ggcgctcccc gagatcccgg cgagcgagct gaccacgacg
      841 attctctacg cggaggcgat ctcggcgttc gagccgttct tcgaggatgg ctcggtccgg
      901 aagctccacg atccgtacgc cccctaccag cgcgaggtga cggcggccct caccggagcg
      961 gactacatca aggcgagccg catgcgcacg gtgctgcaag agcgcatggc cgaattcttc
     1021 agcacgtacg acgtgatcgt cgcgacgaac ttcctgtctg tcgcgccctc gatccacgag
     1081 gacttcacga aaacgctccc ctacccggat ccggtcggcg cggtgggcaa cggctgcgga
     1141 ctccccgcca tcgcccttcc gatggggccg ggcaaggacg gcctgccgac gagcttccag
     1201 atcatgggat ctccgttcga ggaggggctg ctgctcgatt tgggtgaggc gtaccaggga
     1261 gcgacgcgcc atcacctggc ccgtccgccg gtcgcctaga gttgcgtgct atgctcggtg
     1321 gatgtcgcgc gccgcgcgac ggcccggcac gcgggctcgg tcccggcgcg ccggaggtcg
     1381 aacatggcac gaagccaccg tcaatccatg ctcatgaagc gccgcaagcg agcgaagtcg
     1441 ctcgagcggc tgcggaaggc gtatgggaat acgcgacgcg aggagcgggc tcgcgtcgtt
     1501 gagaagctcg ggaagatcgc tccctggttg aaagaagact atctgcaggc aagggcggcg
     1561 caggcgccgc cccgcacacc acacccgtag tttcgcgcgc ggggccgccg tcaatcgaag
     1621 tcgcggtggc cccgcatgtg ctcgaccatc ttgagcatgg cgcgctggat catcatccgc
     1681 gccgcgttcc cagacgccag atcgacttcc tccgcgatct cctcgtactt gaatccgtac
     1741 tcgaaccgca agatgaccgc ctgccgctga cgctcgtcga ggatcttgag cgcggcctcg
     1801 aacgcttccc tttggtccat ggccaccgcc cgctcgtggg gcgtcggctc gtccgtcgca
     1861 atgttcgtcg gcatttcctc ccgctccggc tcgcgcacgc gcgagagcga gatgatgaga
     1921 ttccgcgtca cggtgcgcag gtaatccatc agggccccct cgcgcctgta ctcgaaatcc
     1981 aggagcttcg gatcgacctg ctcgatcacg cgcatgacga tgtcgtccac gtcgtggaga
     2041 gcgcgcacgc ggtgcggaat gtagtgaccg cgcacgagac ggccgagggg cttgaaatag
     2101 cggttccaga gcttcgtccg cgcggcttgg ttgccgtcac ggatctgagc gagcaggaag
     2161 atcgtggtcc ggagcttggg ttcggacccg tcgtcgtcgc cagctagggc ggtaccgtct
     2221 cccagggtgc ctcccctatc ggctcccgaa gacgtgcagc cgatggagct cggccgcgga
     2281 gggctggcgt gaggatagcg gtgtgcgcgc ggctgtcaag gcaccgctcg gtgccaacag
     2341 aacgccccgc cgcgaggagc ggcggggcgc agaacgtacc gatggagtgg agcggccgcc
     2401 tacttgagat gcttcgagac gagcttcgtc atctcgaaca tgctgacctg gcgcttgccg
     2461 ccgaacacgg cgcgaagctt gtcgtccgca ttgatcatcg tgcgcttggt cttgtcctga
     2521 agcttgttct tcttgatgta cgcccaaagc ttcttcgtga cctccgtacg cggaatcgcc
     2581 ttcgcgccga caacctcgga gagggggccg gtcggcgtca gcggcttcat gaacgctgcg
     2641 ttcggcttgc gcttggtgcg agcggcagtc ttcttcttcg tcgcgggctt gctggcgcgc
     2701 ttcttagctg gcatgcacct ctcctttcgt gtgcgttcgc cgatccgaaa ccttgtcggc
     2761 gccgggtgga taactaccac tctcccgggg agagagcaag aggtacaagg gaaatcggag
     2821 gttccggaga ggaaaaatat ggagcggaag acgggatttg aacccgcgac aaccaccttg
     2881 gcaaggtggg gctctaccgg gctgagctac ttccgctcga agacgttctt atgttcggcg
     2941 aagcggaggg gattctaaac cgcccttgga cggggcgtca agcgaccccc gcccctaggc
     3001 gggaggctcc caaccgggac cttggccagg cgcttccgcc tcggcgccct cgccccgttc
     3061 caccgcttcc agccagcgtc cgaatccgct ccccatgcgc ttcgcgccat tcgccgcccg
     3121 atagagctgg acgtacttcc gggccgagcg ctcccaggag aagtcctggg acatcccgtt
     3181 gcgccgcgcc cgcgccatgg agaccggatc ctggtagagc gcgagtgcgc ggtcgacggc
     3241 gtcgaggagc gcctgcggcg tataggggcg aaagaggatc cccgtcccct gggcgagatc
     3301 caccgggccg aacggcgtga ccgtatcggc aaggcccccc gtctcgcgca cgatcgggac
     3361 cgtgccgtag cgcatgctca tcatctggtt gagcccgcac ggctcgtacc gtgagggcat
     3421 gaggaagaag tcggcgcccg cttcgatgcg atgcgcgagc ggatcgttga actccgcgcg
     3481 gaacgcgacc tgccgcggct tccggcgaga ccactcgcgg aacagagtct cgaagcgcgc
     3541 gcttcccctt ccgagaaccg cccacgccgc ggggcgctcg aagagccgct cggccgcttg
     3601 ctccacgaga tcgaacccct tctgctccac gagccgcgcg atgatgccga cgagcggcgc
     3661 accgtcaccg ggctcgagac cgagctcctc gaggagggca gccttgcaga cgcccttccc
     3721 atccagcgac tcggcgtcgt agcgcgccgc gatggcgcgg tcggttctcg gattccactc
     3781 cttcacgtcg atgccgttca ggatcccggt caggtcggcg tgccgcgcgt tcagcacgcc
     3841 ctggagcccg aagccgaatt cctccgacga ctggatctcc tcggcatacc gctcgctcac
     3901 cgtcgtgagc cggtccgcga actggatccc cgccttgagc acgttcatct ttccccagaa
     3961 ctcgaacggc gagagcggat agaagaagcg ctccggaaaa cccgcgatcg ccatggcttc
     4021 gggggcgtac acgccctggt agccgaggtt gtggatggag aggaccgtcg cggtcatctg
     4081 gaagaacgga tcgttcgcgt agagcgtctt catgtaggcg gtcacgagcg ccgtctggtg
     4141 gtcgttcgcg tggatcacgt ccggtacgta cgccgtcgcg cgcatcgcct cgagcgagcc
     4201 gcgggagagg aacgtgaacc gggccgcgtt gtccgcgaac tcttcgctgg tcgagggatc
     4261 gccgtagatc ccgtggcgcc cgaagtaggt ctcgttcgcg aggaagaagt ggtggagatg
     4321 cgtggggtag ggcccgttcc ggagcttgta gacgagcccc ggctgatcct cgcccgagac
     4381 cggtacgtcg atcttcgcca cgagctcgag atcgcttccc ggagggagct cgagatccgc
     4441 gtagcgtggt aggaagaccg cgaccgagca gccgagatcg gcgacggccc ggctcaaggc
     4501 gcccgcgacg tccccgagcc cgcccacctt cgcgaacggc gtgcactcgg gggcgaggat
     4561 cgccgcgcgg aatcgctggg ccatcaagcg cgaagtcctc gcaggtagtc cgccgcctct
     4621 tcaggagccg tggggttgat gtggaacccg ctcccccact cgaagccggc cgccggcgtc
     4681 cgcttcggca tgatctcgag atgccagtgg taggagtcgc gctccgccgt ggtccagggc
     4741 gcggtatgga gcacgaagtt gtaatggggg tgatcgagcg cgcgccggat gcgccccagg
     4801 gtctccttca ggatggacgc gaacgcctgg agatcctggg ggtccgcgtc ctcgtagccc
     4861 gcccggtgcc gtcgcgggag gatccacgtc tcgaacggga agcgcggcgc gtagggctcg
     4921 atggcgagaa agcgctccgt ctcgagcacg acgcgccgcc cgtccttccg ctcgtggtcg
     4981 atcatgtcgc agaacacgca gcgctccttg gcctcccagt gcgcgcggct cccctcgagc
     5041 tcttccatga cgacgcgcgg cacgatcggg gtcgcgatga gctgcgagtg tccgtgctcg
     5101 agcgacgcgc ccgctttccg gcccgcgttc ttgaagacga ggacgtactg cagccgggtg
     5161 tccttcttga gatcgaggat ccgctccttg taggcctcga ggacgcgctc gatctgctcg
     5221 accgggagct cgctcaggtc cttctcgtgc tcgggtgatt ccacgatcac ctcgtgcgcg
     5281 ccgacgccgc tcatccggtc gtagagccct tccccgcgcc gctcgagcgt ctcttcgaca
     5341 cggagcgccg ggaacttgtt cgggaagacg cgcgtcgtcc atccgggctc gttcggcgca
     5401 ccgtggtcgc ggaaggccag gatctcggga ggcgtgtgcc cctcgtggcc gggacagaag
     5461 gggcagctct cgcggccacc gttcgatggg ggcggatcgg tctcctggaa gtcggacggg
     5521 cggaggcggc gttcagagga gaggatgatc cagcggccga cgacggggtc cttgcgaagc
     5581 tcgcgcatga gcggtgcagt ctatcagctt ccacccttgt cgagcacgcg gaacgcgccc
     5641 gaatagccgc tctgaacggc gcgctccttg agcgcctgcg cgtcctcctc gcgcacgaag
     5701 cttccaagcc ggaccttcac gagatcgcca tccttcgcga ggacgtatgg cgcgccgagt
     5761 ttttgcgccg cctcccgcgc gacgcgctcg gcctgcgcgc gatcggggct cgcgaagact
     5821 tgcacgcgcc agacggcggt cgatggggct gaggcctctg gcgctgtgcc cgagggcggg
     5881 acgctcgagg tgctcggcgg cggctcggcc tgcagtgacg tggtggggga aggcgccgcg
     5941 ctcgtccgcg cgggggcgac tgggaggggc ttggcgtccg gattgagcgg gcgctcttcg
     6001 atcggcgccg cacccgcccc cggcacaggg tccgggatcg aggccagctc gtcgggcgtg
     6061 atcacctcgc tcgcctccag gcgcggggcg ggcgccggag cctccgcctg gggttgcgcc
     6121 tcgggcgccg gggcgggggc gctggcctgc ggaggggctg gcgaaggcgc cggctgcggc
     6181 gccggagcat gggagcaccc caggagcgcc atcagagcga atgcggcggc cctagagaag
     6241 cgcggagcca tcgtcctccc tccggagccg ctccacgagc gcacgcacgt cctgcgcccg
     6301 ctctctcggg cagaccaggg tgacgtctcc cgtccggacg atgacgagat cccgcacgcc
     6361 cagggcggcg acgatcccat cttcggcgaa caccacgctc ccggtcacgt cctcgaggag
     6421 ggcacggcct cgcacggcat tcccggcggc gtcccgggca tcgccgccca gggcggccca
     6481 ggagccgagg tcgtcccacc cgaaacgcgc cggcaggacg agcgcgttcc gggtgcgctc
     6541 catgacggcg tagtcgatcg agatcgaggg gacggcggcg tagtagcgct cgagggcccg
     6601 ctccgcgccg ggctcgcccg gccgaggcat gccctcgagg gcggaggcga tctcgggcgc
     6661 gtgcgcgcgg atctccttca gcatgacctc cggcgtgaag acgaacatcc ccgcgttcca
     6721 gaggtgcccg ctcgcgacgt agcgcgcggc cgtctcccga tcgggcttct cgcggaaggc
     6781 cgccacgcgc cgcgcgcgcg atcccttggc gacgggctcc cccatctcga tgtagccgta
     6841 accggtctcc ggacgggacg ccggaatgcc gatgacgacg atcgcgctcc gctcgagcgc
     6901 ggtctgcgcc gcctcctcga gatcgttccg gaacgcgtcc gccggctcga tccggtggtc
     6961 cgaggggagc accacggcca cggcatcggc ccccgaccca tgcagccacc acgatgcgag
     7021 cgcgatcgcc ggagccgtgt tcttccccgt gggctcgccc acgacctgga gctccggcac
     7081 atccggcacc gcggcgcgca ccgcgggcgc gaggtcgcga gcggtcagga tccacgccga
     7141 ggacgcgggc gcgagcgggt cgaggcgcgc gagcgtgtcc gcgagcatcg atcgctcgga
     7201 gaggagcggc aggagctgct tcggccgcgc gcgccgcgag aggggccaga accgctcgcc
     7261 gcggccgccg gccaggatca cgggatagag ccgcatcgac gtcggttcct cctagtacgc
     7321 gccgcgcgcc gagagcaccg cggggatcgt cagcacgagg atcttgagat cgagcgcaag
     7381 cgagtgtcgc tccacgtact cgatgtcgag ccgcatccac tcgtcgaacg agagatcgct
     7441 ccgcccccgg atctgccaca ggcacgtgag cccgggcctc acggaaaggc gccggagcat
     7501 ccacggctcg taccgctcca cctcttcggg gaacggcggc cgcgggccca ccaggctcat
     7561 gtcccccacg agcacgtgcc agagctgcgg cagctcgtcg aggctcgcct tgcggatgat
     7621 cttccccacg cgcgtgacgc gcggatcgtt atggagcttg aacgcgggtc cctccgcctg
     7681 attcaggtgg aggagaagac cgcggagctc ctccgctccg atctgcatgg agcggagctt
     7741 gagaaacgtg aacttccgcc ctccgcgccc cacgcggatg gcgcgatgga acacgggacc
     7801 gggcgagtcg agcttgatcg cgaccgcggc gacgaggatg accggcagag cgagcggagc
     7861 gagaagcagg gtcagcgcga cgtcgagtac ccgctttccg ccgcgacggt acaggctcac
     7921 ggggcgcact gtagcatagg aacggggccc cggcagcgct cgccggggcc ccgtggtctc
     7981 gtggcgggtg gagctacttg agaatggtga tcgccttgcg ctcctcgtcg gcaccggccc
     8041 ggatgcgaac gtagtagacg ccggagctga gcctcccacc gctcgcgtcg atgccgtcaa
     8101 ttcgcacgtc gtggtatccc gccacggcgt cactctcgtc cttgagcgtc cgaacgagcc
     8161 ttccccgcac atcgaatccc gaacatattc gtgaatccac tgtaggaaac ggtccctccg
     8221 cctgccgcga gattgatcac gtacgagatg agcgtcatcg gcgcgctggg gtcctgcgga
     8281 cacgtgacca gagagccgtc acggttatgg tcggtcacca cccacaggtc caccatcgtc
     8341 ggagtgccgt tcggcgccag ccgatcgccg gaatcgtgta cgccgttgcc gttggaatcg
     8401 aggtacatgt actgcgctct tgcgggtgac gcctgaaccg cgagtgctgt gatgaaaagc
     8461 aaagctgtga ctgcccggtt caagaacatg gcgtccccct gagaacgacc atccatgggt
     8521 gagtgacccg gggcatgccc gagttctcat ccgtttcgag acctgcgtgg actacttgag
     8581 tatcgtgatc gccttgcgct cctccgcggt gccggcccgg atgcgaaggt agtagatgcc
     8641 tgagctgagc ctagctccgc tggcgtctgt gccgtcgatc ctcacgtcgt gatatcccgc
     8701 cgcggcatcg ctctcatcct gcagcgttcg cagaagccgc ccatgcacgt cgaagagctg
     8761 gacgttcacg ggaccacgct cctgcgtcgt gaacgtgaga acggcggaag ggttgagggg
     8821 gttgggggtg atgctggccg cgattgcccc acctccgccc ttgacgctga ccgtgagcgt
     8881 ggccgcacag atgggccccc cagagagaag attcccggtc agtgtcacct ggatctgctg
     8941 cgtgccggtg acgttcgaga agagctggcg aaggtcgtcc ttccggaagc aggcggtgat
     9001 ctccgaaagg ccatcgccgt cgcggtcgcc cccgacggag gtcttgtccg agagcgcgcg
     9061 gatttcctgg acggatcccg tgcctgccga tcggaggacg atgctcgaga gatcgatgtc
     9121 ggtgacgtcg tagtcctgag cctcgatttg ggcgcaccag gccggctttc ccgactggag
     9181 gttgatctgc ttgttgcctc cggccacgaa
//
LOCUS       DASTKC010000232        12136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1378219, whole genome shotgun sequence.
ACCESSION   DASTKC010000232 DASTKC010000000
VERSION     DASTKC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378219"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFR25_09735"
     CDS             <1..721
                     /locus_tag="VFR25_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9941365.1"
                     /translation="IISLAWLADSQRAGRPAVLASASFGLTSLGLVLSGARGAWLGLC
                     LGAAAMVLVQRDPSAMRRAVRLGAALLALAAITLLSGVGGFLYERIAGSASHPASWAQ
                     RYDALLAVASWGRRIPLIGLGFGGATELTARMGMLLPNLENEYLRFLLAAGVAGPLVL
                     LVTGVRRIRSAIRLPSGPVRTAGIGCLTALFVNMGTYNLFSWSIAPSLFMALAVVTLR
                     SVREPAPDGFAVAHRMAVRPA"
     gene            718..1824
                     /locus_tag="VFR25_09740"
     CDS             718..1824
                     /locus_tag="VFR25_09740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9941366.1"
                     /translation="MKPLRVCLVLWSGGIGGAETWMLALARSLRRRGVDAGVLFIGSP
                     EPLASQLDTVPWSTLGLERGAQVILKPRTFASLVDRIGPDAALLPATGYLARMLRIGG
                     YRGRIAAIEHGSALQLGHQIWWRRWVRSIDMLSGRGVSDVLIAPSDFMLHRLRRLSRA
                     TRYARIYPGIRLDRFTPGSGAAGTSRDITIGCAARLIDGKGVDVLLKAVARLGVATPV
                     ALQIAGDGPMKRELIQLARDLGLERVTRFLGSVPDMPAFWRSCDVAVVPSSTWVESFG
                     MVAAEAMACGMPVLASRQGGLPELVLDGSTGYLFEPGDHEQLADLLARYAKDPALRGA
                     HGHAAMEHSRARFDISRSARELEELLRPEIIHAN"
     gene            1814..2995
                     /locus_tag="VFR25_09745"
     CDS             1814..2995
                     /locus_tag="VFR25_09745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941367.1"
                     /translation="MRTESTPKSGRIVLVAPTDYRRFAFGGTLSYIQEFLAGLEKDVA
                     KQVSLIGVEPADSTGHDPVREIRGRSFPFLSVCRVGDERIPLRLRFAAGLLAHRKMIH
                     SLDARLFYAHSAESALALSFVSRKTPIVLHCHGTTNTIERSRFWYGRLAPIRHAYEDW
                     ILRPAMSRSSKVVVTADSVEYERFVNRHEDRLEGRASRVGGVVDIGLFSPDNGASRDR
                     SFLALIAVGRVERPKGYDLLLDAVHRLRLEGVGVRLTIVGEGSDQPRLMKKVEDLDLG
                     GHVEFTGYMNRLQLADAMRRSDLYVSGSEQEGFSLALLEALASGLPVVATDVGGVAEV
                     VQDGTTGYRCTSRDPVEFARLVKLAVSRMESMRAACCSAAAAYGSDSIAARIYAELQH
                     A"
     gene            3195..5177
                     /locus_tag="VFR25_09750"
     CDS             3195..5177
                     /locus_tag="VFR25_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HET9941368.1"
                     /translation="MAKVLDSVRTEGLVATYKKVTERLATPQPLGYSLAGTIIDVGED
                     WDGLHVGMAVACGGASASHAEVVSVPKNLVVPIPHGVPMHDACYATLASIPLHAIRLG
                     EVAIGDRVLVIGLGLMGQLAARLCAGAGARVFGVDPDRSRADLALHWCAEQVDTRLDR
                     STALQILDWSRGRGADVVLITAGGADSRPLGLAGEAARDRAKVVVVGAVGLDVPRESF
                     YEKELRLVVSRSYGPGRYDPEFEEKGYAYPPGYVPWTERRNMEEILDLLQCGRLPLDG
                     LRGATIPFERAPEAYDLLRSDSPPISIVLDYGSAKPHAVPGTSSHASQAHAPHTEPST
                     HLPRSLRVSFIGMGNFASSYLLPAAKQTAESLEHVVTQTPLKAEHARKRGGFRSASTD
                     GLAAIEDPNTDVVFVATRHDTHARWVEAALRAHKAVFVEKPLALTARDYERIATLQRA
                     TQGRLMVGFNRRFAPATMWALDALGSNRTGLRITYRINAGALPPHHWLLDPDTGGGRL
                     IGEGCHFVDYACFLADASPVSIDARALDAAGESFHIEIAFSNGASCGIDYLSRGDASL
                     AKERIEIHRSGLSIVIDDFRTASLHRGGKRAAMKRWKGRDKGHQAEVRAFLEAVVSGA
                     PTPIPEEESLRSTALTLAAAMSLREGRAIHRDEWPR"
     gene            5174..6403
                     /locus_tag="VFR25_09755"
     CDS             5174..6403
                     /locus_tag="VFR25_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941369.1"
                     /translation="MTRAKASTIIPLDIAHALLARTRARGWAGADPYDGLEGPIGALV
                     RPLGPLPRFALSQSVLRSPLARAAAMPRATVNPKALGLFLGAVARLPAGSAGSRIGSE
                     LEEAIASRATPHRDALGWGYPFGWQSRHFWAPPHLPNAVVTSTVAWHLFEYSDRHESN
                     RAQSMALAGARFLSSALARSKAEGGEAIAYTQKDRTQVINVSALAARVLARAAAVEES
                     PRFRENASRLVGFVLAQQREDGSWPYATDPRGQWEDSFHTGYILESLIAVQAYGIDVP
                     HAALRAGFEAYGRFFGSDGEARLLPRADSVLDAHAAAQGILTYAALAGWNEGPRSLRD
                     TAAGRSLLIALWSVEQLWLSERGHFAYRVRRGRRDEREFTRWVTAWMCLAMTVAAELT
                     AHASATPPLTTQASEVA"
     gene            6316..7488
                     /locus_tag="VFR25_09760"
     CDS             6316..7488
                     /locus_tag="VFR25_09760"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HET9941370.1"
                     /translation="MPRHDGRGGADGARKRDAPFDNTSFGGRLRVWIDVDNAPHVQFF
                     RPVIRRLHELGADVQVTARDRTYVPQLLHSFGIRHTVIGHGRPESPIAKARALAARTA
                     LLMRYAAGKKFDVAVGHGSRALAPAARLSGVKNLTMFDYEHVSTWIFRTFCDRILVPR
                     ATLSSMTRLEPAGRWVPFDGFKEEVYLAEYVVDPSIRVRLGVKPEDILVALRPPATTA
                     HYHDPKSEGMLRAITARLGSSTNVRTVWLRRDLADPIPNLEWVPSFIVPRRPLDGPSL
                     LAEADVVISGGGTMTREAALLGTPAYSIFTGATGAIDEELSRRGSLVLVRSVEDAEKI
                     PFVKKSGSSAAPGCTPALREFVLDQIQDLARSKGRARAGVADEASGKGGQVGSSIR"
     gene            7469..8584
                     /locus_tag="VFR25_09765"
     CDS             7469..8584
                     /locus_tag="VFR25_09765"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9941371.1"
                     /translation="MGVPFVDLREHHRAIRAELDLAVRDVIESATFVLGPAVQDFESD
                     FAQYLGVDHAVGTSNGTTALQLALLALGVGQGDEVIVPAQTFIATAEAVAHTGARPVF
                     VDVLESTACMDPAALDAAITSRTRAIVPVHLFGQAAELEPILDVARTRGIPVIEDACQ
                     AHGATYHGRRVGSFGVASCFSFYPGKNLGAFGEGGAVVTNDDHLAARVRRLRDHGQSQ
                     RYYHAEVGYNARMEGIQGAVLGVKLRYLDRWNESRRAHAAYYREWLAASGVRLPVEAS
                     RREHVYHLFVVRSEDRNTLREHLTHQGIQTGLHYPVPLHLQPAFSGMGFRVGAFPTAE
                     AWAREGLSLPMFAELTRAQLDEVIEGVKSHNLVANIH"
     gene            8753..10168
                     /locus_tag="VFR25_09770"
     CDS             8753..10168
                     /locus_tag="VFR25_09770"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9941372.1"
                     /translation="MTRSRHVAVVSLAILLDLLAVSAGFLLPALLRFTPVALLEAALA
                     PSGATTLMLGALLWVAMLSARDAYGPRVMVSKVDQALRVLEAGVAAWVLTHLLAFVTK
                     TPVPFESRLAVGLSLPLVLVALTTVRLALVRPLARRAYERLVRGPRLVVGDSERAERL
                     AVDLEERDPEGRPTLIVPLAPMSVDDASRLVEAYGFGEVLIEPTGRSLEEVLDIAFAF
                     LDARVDVRVVSSRFEIVVGRSAIGALDGVPVIRFRRFDLAGPEVAVKRLVDVVGAALG
                     LVLLSPVLAAIAIAIRTTSSGPVLFRQERVGQGGRAFTMLKFRTMEDGNDPKVHQDYL
                     RQYMHGGEAAAAEVAADGTRIYKLTRDPRVTPLGAWLRALSLDELPQLWNVLRGEMSL
                     VGPRPCMPFEWTMYRPWQKRRLDVKPGCTGLWQVTARSRVGFEEMVILDLHYAHHGSI
                     GGDLGLIARTVPAMLRGRGGY"
     gene            10254..11381
                     /locus_tag="VFR25_09775"
     CDS             10254..11381
                     /locus_tag="VFR25_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9941373.1"
                     /translation="MRVGVIGCGYWGPNLIRNLLENRKCTGVTVCDPDSASLARARQR
                     FPTVECVGSVDELLRDRAVEAVLIATPVTTHFALARACLRSGRHTFVEKPLTATTAEA
                     EELIRIADRAGVTLMVGHTFEYSPPVVKIREIIESGSMGEIFYISAIRVNLGIHQRDV
                     SVVWDLAPHDLSMLFYWLGEVPTAVSMTDGAFVQPLISDVAFINLQFESGKIANIQAS
                     WLSPSKLRQTTIVGSHKMLIYDDTNAMERVKVFDRGVEFKDPQTFNEFTLTYRTGDIV
                     SPHVSAAEPLGIEMDHFLECARTGATPRTDGESGLRVVRVLEAIERSGRNGNRLERID
                     WERTGVAPGVLDGVGNGNGRRAAGVPALVSGAMAASRGTES"
     gene            11378..12115
                     /locus_tag="VFR25_09780"
     CDS             11378..12115
                     /locus_tag="VFR25_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET9941374.1"
                     /translation="MSPGVVEDAVHVIEAIRRAAAEGARGAEEPIPGIQIYPGVEIGD
                     GAVLEPPLVLGKPPRGCEPGELRLVIGHGAVIRPFTTIYAGTTIGDRFQSGQGASIRE
                     DNVLGDDVSVGTNAVLEFGNRVGHHVRIHSGCFLEMATVEDFVFLGPHVVFTDDPHPM
                     GCPHYKECLGGPTVRAMAKVGANSTLLPGIVIGRGALIGAGSVVTKNIPDEVVAAGNP
                     AVVIKRIDELKCHPGFFARPYEWEPYR"
BASE COUNT         1826 a         4045 c         4209 g         2056 t
ORIGIN      
        1 catcatcagc ctggcctggc tggccgactc gcagagagcg gggaggcccg ccgttctagc
       61 ctccgcatcg ttcggcttga cctcgctggg gctcgtgctg tcgggcgctc ggggagcgtg
      121 gttgggactg tgcctcggcg ccgccgcgat ggtcctcgtt caaagagatc cgtcggccat
      181 gcggcgtgcg gttcgcctcg gtgccgcgct cttggccttg gccgcgatca cactgctctc
      241 gggtgttggg ggcttcctgt acgagcgaat cgcgggctct gcttcacatc cggcgtcctg
      301 ggcgcagcga tacgacgcct tgctggccgt ggcgagctgg gggcgtcgga ttcctctgat
      361 cggactgggc ttcgggggtg cgacggagct gaccgcgaga atgggaatgc tgctccccaa
      421 tctcgagaac gagtacctcc gattcctctt ggcggcaggc gtggccggtc ccttggtcct
      481 tctcgtgacg ggtgtccgac ggatccggtc ggcgatccgc cttcccagcg ggccagtgcg
      541 cacggcaggc attggatgcc tgacggccct cttcgtgaac atgggcacgt acaacctgtt
      601 ctcctggtcg atcgccccgt cgctcttcat ggcgctcgcc gtggtcaccc tgaggagcgt
      661 tcgcgagcca gctcccgatg gctttgcggt ggcccatcga atggcggttc ggccggcatg
      721 aagccgctcc gagtgtgcct cgtcctttgg tcgggcggga ttggaggggc cgagacctgg
      781 atgcttgcgc tcgctcgaag cctgaggcgg cgcggtgtcg atgccggtgt tctcttcatc
      841 ggctcaccgg aaccgctcgc gagtcagctc gacacggttc cgtggagcac gctgggactg
      901 gagcgcggag cacaggtaat cctgaagccg cggactttcg cgtcgctcgt cgaccgaatc
      961 gggcccgatg cagctctctt gccggccacc ggctacctcg cccgcatgct ccgcatcgga
     1021 ggctatcgcg gccggatcgc cgccatcgag cacggaagcg ccctgcagct cgggcaccag
     1081 atatggtggc gccgatgggt acgaagcatc gacatgctga gcgggcgagg cgtctcggac
     1141 gtgctcatcg cgccgagcga cttcatgctt catcgattgc gccgcctgtc gcgcgccacc
     1201 aggtacgcgc ggatctatcc cggtattagg ctcgatcgct tcactccggg gagcggagcg
     1261 gccggcacgt cgcgggacat cacgatcggc tgcgccgccc ggttgatcga cggcaagggt
     1321 gtggacgtcc tcctgaaggc ggtggctcgg ttgggcgttg ctaccccggt ggctctgcag
     1381 atcgcagggg atgggccgat gaagcgcgag ctgatccagc ttgcccgtga tctgggattg
     1441 gagcgagtca cgcggttctt gggaagcgtc ccggacatgc ccgctttctg gaggtcctgc
     1501 gatgtcgcgg tcgtgccctc cagcacgtgg gtcgagtcgt tcggaatggt tgccgccgaa
     1561 gcgatggcat gcgggatgcc cgtgctcgcc tcacggcaag ggggacttcc cgagctcgtc
     1621 ctggacggat cgacgggcta cctgttcgaa cctggggatc acgagcagct ggcggatctc
     1681 ctggcacggt acgccaaaga tccggcgcta cgcggcgctc atggccacgc ggcaatggag
     1741 cattcgcggg cacgattcga catctcgaga agcgcgcgtg agctcgagga gctcctccgg
     1801 cccgagatca tccatgcgaa ctgaatccac gcccaagtcg ggccgtatcg tcctcgtcgc
     1861 tccgaccgac taccgacgat tcgcctttgg ggggacgctg tcctacatcc aggaattcct
     1921 ggcgggcctc gagaaggacg tcgcgaagca ggtatcgctg atcggagtgg agcccgcgga
     1981 ctctaccggc catgaccctg tccgggagat ccgggggcgc agctttccgt tcctgtccgt
     2041 gtgccgggtg ggcgacgaga ggattccgct tcggttgagg ttcgcggcag gactactggc
     2101 gcatcggaag atgattcatt ccctcgacgc gaggcttttc tacgcccaca gcgcggagag
     2161 tgcgctggca ttgagcttcg tgtctcggaa gacgcccatc gtcctgcact gtcacggcac
     2221 gacgaatacg atcgagcgat cgagattctg gtacggacgc ctggcgccca ttcggcatgc
     2281 gtacgaggac tggatcttga ggcccgcgat gagccggtcc tcgaaggtcg tcgtcacggc
     2341 cgactccgtc gagtacgaac ggttcgtgaa tcgacacgag gatcgcctag aggggcgggc
     2401 cagccgggtt ggaggagtcg tcgacatcgg cttgttctct ccggacaacg gcgcatccag
     2461 ggaccgaagc tttctagcgc tgatcgcggt ggggagggtg gagcgtccca aagggtacga
     2521 cctcctcctc gacgcggttc atcgcctgag actggagggc gtgggtgtca ggctgaccat
     2581 cgtcggcgaa ggctccgacc aaccgagatt gatgaagaaa gtcgaggatc tagacctggg
     2641 aggccatgtg gagttcaccg gctacatgaa ccgacttcaa ctggcggacg ccatgaggag
     2701 atcggatctc tatgtgagcg gaagcgagca ggaagggttt tcgctcgccc tgctcgaagc
     2761 cctcgccagc gggctcccgg tggtggcgac ggatgtgggc ggtgtcgccg aggtcgttca
     2821 ggacggaacg accggatatc gctgcacgtc gcgcgatccc gtggaattcg ctcggctcgt
     2881 gaaactcgcg gtttcccgga tggagagcat gcgagcggca tgctgttccg cggccgctgc
     2941 ctacggttcc gactcgatcg cggcccgaat ctacgcggag ctccagcatg cgtgagccgg
     3001 tgcgagtccg aaccattggc ctggatcagt ccggcggccg cgtcctggtc gagtcggttc
     3061 cggagccacg cgccggacga ggaacgatct tggtccgggt ctcccgatcc gtgatctcgt
     3121 ccggaaccga gcggtccaag ctcgaacttg ccggagcttc gactctcgag aaagcacgac
     3181 gtcgccccga ccaggtggcg aaggtccttg actccgttcg caccgaaggc cttgtcgcaa
     3241 cctacaagaa agtcaccgag cgtctcgcga cgccgcagcc cctcgggtac tcgcttgccg
     3301 gaacgatcat cgacgtcggt gaagactggg acggcctcca cgtgggtatg gccgtcgcct
     3361 gcggcggtgc gagcgcgagc cacgccgagg tcgtgtcggt gccgaaaaac ctcgtcgtac
     3421 ccatccccca tggcgttccc atgcacgacg cttgctacgc cacgctcgca tcgatcccgc
     3481 tccacgcgat ccgcctgggg gaggttgcga tcggggatcg ggtcctcgtg atcgggctcg
     3541 gcctgatggg acagctcgcg gcgcgcctct gcgccggggc gggggcgcgc gtgttcgggg
     3601 tcgatcccga tcgcagccgc gccgatctgg cgcttcattg gtgcgcggag caggtcgaca
     3661 cgaggctgga tcgctcgacc gcgctccaga tcctcgactg gagccggggc cgtggagccg
     3721 acgtggtcct catcaccgcc ggaggagcgg acagccggcc cctcggtctt gcaggcgaag
     3781 cggcgcgcga tcgcgcgaag gtcgtggtcg tgggcgcggt cggtctcgat gttccaaggg
     3841 agagcttcta cgagaaagag ctgcggctcg tggtctcgcg gtcctacggg ccgggtcgat
     3901 acgaccccga gttcgaggag aagggatacg cctatccccc cggatacgtc ccctggacgg
     3961 aacgacggaa catggaggag atcctcgatc tcctccagtg cgggcggctc ccactcgatg
     4021 gacttcgggg cgccacgatt ccgttcgagc gcgctcccga ggcgtacgac ctcctgcgct
     4081 ctgactcgcc tccgatctcg atcgtgctcg actacgggtc ggcgaagccg cacgcggtgc
     4141 ctggtacgtc gagccacgcg tcgcaggcgc acgcaccaca cacggaaccg tccacgcatc
     4201 tgccacgatc cctccgggtc tcgttcatcg gcatggggaa cttcgcgtcc tcctatctgc
     4261 tccccgcggc gaagcagacc gcggaatcgc tcgagcatgt cgtcactcag actccgctca
     4321 aggccgagca cgcgcggaag cgcgggggct ttcgctccgc ctccaccgac ggtctcgccg
     4381 cgatcgagga cccgaacacc gacgtcgtat tcgtcgcgac ccgtcacgac acgcacgcgc
     4441 gctgggtcga ggcggcgctc cgcgcccaca aggccgtctt cgtcgagaag ccgctcgcgc
     4501 tcacggcccg cgactacgag cggatcgcga cgctccagag ggcgacccag gggcggctca
     4561 tggtgggctt caaccggcgt ttcgcgcccg cgacgatgtg ggcgctcgac gcgctgggat
     4621 cgaaccgcac ggggctccgc atcacctacc ggatcaatgc gggagccctt ccgccgcacc
     4681 actggctcct cgatcccgac acgggcggcg gtcgtctcat cggcgaaggc tgccacttcg
     4741 tcgactacgc ctgcttcctc gcggacgcat ccccggtctc gatcgacgcg cgagcgctgg
     4801 acgccgccgg cgagagcttc cacatcgaga tcgcgttttc gaacggggcg tcctgcggga
     4861 tcgactatct ctcccgcggc gatgcgtcgc tcgcgaagga gcggatcgag atccaccgct
     4921 ccggcctctc gatcgtgatc gacgacttcc gcactgcgtc tctgcatcgg ggagggaagc
     4981 gcgctgccat gaagcggtgg aaggggcgcg acaaaggcca ccaggcggaa gtgcgcgcct
     5041 tcctcgaggc ggtggtatcg ggcgctccga cccccattcc cgaggaggag tccctccgat
     5101 ccacggcgct caccctggct gccgcgatgt cgctccgaga gggcagggcc atccatcgcg
     5161 acgagtggcc tcgatgactc gagctaaggc gagcacgatc atcccgctcg acatcgccca
     5221 cgccctcctg gcgcgcacgc gggcacgagg ctgggccggc gcggatccgt acgacggtct
     5281 cgaggggccg atcggagcgc tcgtgaggcc gctcggaccg cttccccggt tcgcgctcag
     5341 tcaatccgtc ctccgctcac ccctcgcccg cgccgcggcg atgccgcgcg cgacggtgaa
     5401 tcccaaggcc ctcggcttgt tcctcggagc cgtggcacgc cttccggcgg gcagcgccgg
     5461 ctcgaggatc gggtccgagc tcgaggaggc gatcgcgtcg cgcgcgaccc cgcaccggga
     5521 cgccctcggg tgggggtatc cgttcggctg gcaatcgcgg cacttctggg ccccgccgca
     5581 ccttccgaac gcggtggtca cgagcacggt cgcatggcat ctcttcgagt actccgaccg
     5641 ccacgaatcg aaccgagcgc agtcgatggc tttggccggc gcgcgcttcc tttcatcggc
     5701 gctcgccagg agcaaggccg aggggggcga agcgatcgcc tacacacaga aggaccggac
     5761 tcaggtgatc aatgtgtccg cgctcgccgc gcgcgttctg gctcgcgcgg cggccgtgga
     5821 ggaatcgccg cgattccgcg agaacgcctc acgcctcgtg ggcttcgtgc tcgcccagca
     5881 gcgcgaggac ggctcctggc cgtacgccac ggatccccgc ggccagtggg aggattcctt
     5941 ccacacgggc tacatcctcg aatcgctcat cgcggtccag gcgtacggca tcgacgtgcc
     6001 gcacgcggcg ctccgtgctg gcttcgaagc ctatgggcgc ttcttcggat cggacgggga
     6061 agcccggctc cttccgcgcg ccgactccgt gctcgacgcg cacgccgcgg cccaggggat
     6121 ccttacctat gccgcactcg ccggctggaa cgaaggaccg cgatcgctca gggatacggc
     6181 ggccggacgc tcgctcctca tcgccctgtg gtcggtcgag cagctttggc tgtcggagcg
     6241 gggccacttc gcctatcgtg tgcggcgggg gcgcagggac gagcgcgaat tcacgcggtg
     6301 ggtcaccgcc tggatgtgcc tcgccatgac ggtcgcggcg gagctgacgg cgcacgcaag
     6361 cgcgacgccc cctttgacaa cacaagcttc ggaggtcgcc tgagagtctg gatcgacgtc
     6421 gacaacgccc cgcacgtcca gttcttcagg ccggtcatcc gccgactcca cgagctcggc
     6481 gcggacgtgc aggtgacggc ccgcgaccgc acctacgttc cgcagctcct ccattccttc
     6541 ggcatccggc acaccgtcat cggtcacggc cgcccggaaa gcccgatcgc gaaggcgcgg
     6601 gccctcgccg cgcgcacggc gctgctcatg cgctacgcgg ccgggaagaa attcgacgtc
     6661 gccgtggggc acggctcgcg cgcgctcgcg ccggccgcga ggctctccgg cgtgaagaac
     6721 ctcacgatgt tcgactacga gcacgtctcc acctggatct tccgcacgtt ctgcgaccgc
     6781 attctggttc cgcgcgcgac cctgagcagc atgacccggc tcgagcccgc aggccggtgg
     6841 gttccattcg acggtttcaa ggaggaggtc tacctcgcgg agtacgtggt ggacccgagc
     6901 atccgcgtcc gtctgggggt gaagcctgag gacatcctcg tggcgctccg tcctcccgcg
     6961 acgaccgccc actaccacga cccgaagagc gaggggatgc tccgcgcgat caccgcgcgg
     7021 ctcggatcgt ccaccaatgt tcgcaccgtc tggctcaggc gggatctggc ggatccgatc
     7081 ccgaatctgg agtgggtccc gagcttcatc gtgccgcgac ggccgctcga cgggccctcg
     7141 ctcctcgccg aggcggacgt cgtgatctcg ggtggaggca cgatgacgcg cgaggcggcc
     7201 cttctgggaa cgcctgccta cagcatcttc accggggcca ccggagccat cgacgaggag
     7261 ctgagccgcc gtggctccct cgtgctcgtg cggagcgtgg aagacgcgga gaagattcca
     7321 ttcgtgaaaa agtccggaag cagcgccgcg cctggatgca ctccggcgct tcgcgagttc
     7381 gttctcgatc agatccagga tctcgcgcgg tcgaagggcc gcgcacgggc gggggtcgcc
     7441 gatgaggcga gtgggaaggg agggcaggtt gggagttcca ttcgttgatt tgagagagca
     7501 ccacagggcc atccgagcgg agctggacct ggccgtccgg gatgtcatcg agagcgcgac
     7561 gttcgtcctc ggccctgccg ttcaggattt cgaaagcgac ttcgcccagt acctgggcgt
     7621 cgaccatgct gtcggcacga gcaacggaac gaccgcgctc cagctcgcgc tcctggcgct
     7681 gggcgtcggc cagggggacg aggtcatcgt ccccgcgcag accttcattg ccaccgccga
     7741 agccgtcgcc catacgggcg cacgtcccgt cttcgtcgac gtgctcgaat ccacggcgtg
     7801 catggacccg gccgccctcg acgccgcgat cacctcgcgg acgcgcgcga tcgtacccgt
     7861 gcacctcttc ggacaggcgg ccgagctcga gccgatcctc gatgtcgcac ggacgcgcgg
     7921 catcccggtg atcgaggacg cctgccaggc acacggcgcg acctatcacg gccggcgggt
     7981 cgggagcttc ggcgtcgcct cctgcttttc cttctacccg ggaaagaacc tcggcgcctt
     8041 cggcgaaggg ggcgcggtcg tgaccaacga cgaccacctg gcggcgcgcg tgaggaggct
     8101 gcgcgaccac gggcagtcgc agcgctatta ccacgccgag gtcggctaca acgcgcgcat
     8161 ggagggaatc cagggggcgg tgctcggagt gaagctgcgc tacctcgacc ggtggaacga
     8221 gtcgaggcgc gcgcacgccg cctactatcg cgagtggctc gcggcctccg gagtccggct
     8281 tccggtcgaa gcgtcgcggc gcgagcacgt ctatcacctc ttcgtcgtgc gaagcgagga
     8341 tcgaaacacc ctgcgcgagc acctgacgca ccagggaatc cagaccgggc tccactaccc
     8401 ggtgccgctt catctccagc cggcgttctc gggcatgggc tttcgcgtgg gagcattccc
     8461 gaccgcggag gcctgggcga gggaggggct gtccctcccc atgttcgccg agctgacgcg
     8521 cgcgcagctc gacgaggtca tcgaaggggt gaaatcccac aatctggtgg caaatattca
     8581 ttgacgccgc gcggatgcgg cgattcactg cgaggcgtgg gggcctgagg cctctgcgtc
     8641 acaacatcat cggcggcccg gatgccgccg ggaggaaggg ctgaaccagg aaggtaccgc
     8701 ccgtctcgag gctgagcccg ccttggcgct ggctcgcccg gcacccgcct ccgtgacgcg
     8761 ttcgcgtcac gtcgccgtcg tttccctcgc catccttctc gaccttctcg ccgtgtcggc
     8821 cgggttcctc cttccggcac tccttcgatt cacgcctgtc gcgctcctcg aggccgcgct
     8881 cgcgccgagc ggcgcgacga cgctcatgct cggagctctc ctctgggtgg cgatgctgag
     8941 cgcgagggat gcctacggac cgcgcgtgat ggtctcgaag gtggatcagg cgctccgggt
     9001 gctcgaggcc ggcgtggccg cctgggtgct gacgcacctc ctcgccttcg tgacgaagac
     9061 gccggtgccc ttcgagagcc gcctcgccgt ggggctctcg ctgccgctgg tgctcgtcgc
     9121 tctcacgacc gtgcgcttgg ctctggtgcg gccgctcgcg cgacgggcgt acgagcgcct
     9181 cgttcgcggg ccgcggctcg tcgtgggcga ttccgagcgg gcggagcggc tggccgtgga
     9241 tctcgaggag cgcgatcccg aaggccgccc cacactcatc gtcccgctcg cccccatgtc
     9301 cgtggacgac gcgtcgcggc tcgtcgaggc gtacggcttc ggcgaggtgc tgatcgagcc
     9361 gacggggcgg agcctcgagg aggtgctcga catcgccttc gcgttcctcg acgcgcgggt
     9421 cgacgtgcgc gtggtctcga gccggttcga gatcgtcgtc ggccgctcgg cgatcggcgc
     9481 cctcgacggg gttccggtga ttcgcttccg ccgattcgac ctggccggcc ccgaggtcgc
     9541 cgtgaagcgg ctcgtggacg tcgtgggcgc ggccctgggc ctcgtgctcc tctcgcccgt
     9601 cttggccgcg atcgcgatcg cgatccgcac gacatcgtcc ggtcccgtgc tcttccgtca
     9661 ggagcgcgtg ggacagggcg ggcgcgcgtt caccatgctc aagttccgca cgatggagga
     9721 cggaaacgat ccgaaggtgc accaggacta tctgcgccag tacatgcacg gcggagaggc
     9781 cgcggccgcc gaggtcgcgg cggacggcac gcggatctac aagctcacgc gcgatccgcg
     9841 cgtgactccg ctcggggcgt ggctgcgcgc gctctcgctg gacgagctgc cgcagctctg
     9901 gaacgtgctc cggggcgaga tgagcctcgt cgggccgcgg ccctgcatgc cgttcgaatg
     9961 gacgatgtat cggccgtggc agaagcggcg gctcgacgtg aagccgggct gcaccggact
    10021 ctggcaggtc acggctcgga gccgtgtcgg attcgaggag atggtgatcc tggatcttca
    10081 ctacgcgcac catggctcga tcggcggcga tctcggcttg atcgcccgta cggtgccggc
    10141 gatgctgcgc gggcgaggcg ggtactaggg ggctgtgagt gggctgctcg ggcgccgcgt
    10201 ggggacgcgg ccgcgagcgg atgcgcttgg gttgggccag cggggagggg acgatgcgcg
    10261 tcggggtgat cgggtgcggg tactggggtc cgaatctcat tcgtaatctg ctcgagaacc
    10321 ggaagtgcac gggggtcacc gtgtgcgatc cggattccgc gtccctcgcg cgggcgcggc
    10381 aacgattccc gaccgtggag tgcgtcgggt ccgtggacga gctcctgcgc gaccgcgcgg
    10441 tggaagccgt cctcatcgcg acgccggtca ccacccactt cgccctcgca cgggcgtgtc
    10501 tgaggtccgg ccgccatacg ttcgtcgaga agcccctcac ggccacgacc gccgaggccg
    10561 aggagctgat ccgaatcgcc gatcgcgcgg gagtcacgct catggtgggg cacacgttcg
    10621 agtactcgcc gccggtcgtg aagatccgcg agatcatcga atcgggcagc atgggcgaga
    10681 tcttctacat ctcggccatc cgcgtgaacc tgggcatcca ccagcgggac gtgagcgtgg
    10741 tgtgggacct cgccccgcac gatctgtcca tgctcttcta ctggctcggt gaagtgccga
    10801 cggccgtgag catgaccgac ggagcgttcg ttcagccttt gatctcggac gtggccttca
    10861 tcaatctcca gttcgagagc gggaagatcg cgaacatcca ggcgtcctgg ctctccccgt
    10921 ccaagctgag gcagacgacg atcgtcggct cgcacaagat gctgatctac gacgacacga
    10981 acgcgatgga gcgcgtgaag gtgttcgacc ggggcgtcga gttcaaggat ccgcagacct
    11041 tcaacgagtt cacgctgacc taccggaccg gtgacatcgt ctcgccgcac gtgagcgcgg
    11101 cggagccttt ggggatcgag atggatcact tcctcgaatg tgcgagaacc ggagcgacgc
    11161 cgagaacgga tggcgagtcg ggtctgcgcg tggttcgcgt gctcgaggcg atcgagcgga
    11221 gcggacggaa cgggaaccgg ctcgagcgga tcgactggga gcgcaccggt gtcgcgccgg
    11281 gcgtgctcga cggcgttggc aacggaaacg ggcggcgggc cgcgggagtc ccggctctcg
    11341 tgagtggcgc gatggccgcg tctcggggaa ccgagtcgtg agcccgggcg tcgtggagga
    11401 cgcggtccac gtgatcgagg cgatccgtcg tgccgccgcc gaaggggcgc gaggggcgga
    11461 agagccgatt ccgggcatcc agatctaccc cggcgtggag atcggggacg gcgcggttct
    11521 cgagccgccg ctcgttttgg ggaagccgcc tcgcgggtgt gagccgggag agctgcgcct
    11581 cgtgatcggc cacggcgcgg tgatccggcc gttcacgacg atctacgcgg gcacgaccat
    11641 cggcgatcgc ttccagtcgg gccagggcgc ctcgatccgc gaggacaacg tgctagggga
    11701 cgacgtctcc gttggcacga acgcggtcct cgagttcggg aatcgcgtgg ggcaccacgt
    11761 ccggatccac tccggatgct tcctcgagat ggccacggtc gaggacttcg tgttcctcgg
    11821 tccgcacgtc gtcttcaccg acgacccgca tccgatgggc tgcccgcact acaaggagtg
    11881 cctcggcgga cccacggtgc gcgcgatggc gaaggtcggc gcgaactcga cgctcctgcc
    11941 cggcatcgtg atcggacgcg gcgcgctgat cggtgcgggc tcggtggtga ccaagaacat
    12001 ccctgatgaa gtcgtcgccg cgggcaatcc cgccgtcgtg atcaagcgga tcgacgagct
    12061 caaatgtcac ccgggattct tcgcgcggcc ctacgagtgg gagccgtacc ggtaggttcc
    12121 taccaggcca ccttcg
//
LOCUS       DASTKC010000233         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1857839, whole genome shotgun sequence.
ACCESSION   DASTKC010000233 DASTKC010000000
VERSION     DASTKC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857839"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1039)
                     /gene="pstA"
                     /locus_tag="VFR25_09785"
     CDS             complement(143..1039)
                     /gene="pstA"
                     /locus_tag="VFR25_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9941375.1"
                     /translation="MTPAAEAGAVTPVWVDRTPRSVHLLRAVTNRVVIGACVLAACVV
                     VLPLVLIMGCLLANGVAGLNLAFFTHMPKPVGEAGGGMANAIVGTLLVVGVAALIALP
                     LGIAAGVYLSEYGRGRFATLVRYTTDVLSGVPSIVVGVVAYGLVVIPMGRFSGFAGSV
                     ALAILMLPPIIRATEEMVRLVPQSYRQAALALGAPRWKTTQQIVLPAARAGIVTASML
                     AVARAAGETAPLLFTALGNRFWSLRMDQPIATLPVFIYDYAKAPYTDWNRQAWTGALV
                     LVLLVTLISLMLRLTVRRVRDR"
     gene            complement(1036..1986)
                     /gene="pstC"
                     /locus_tag="VFR25_09790"
     CDS             complement(1036..1986)
                     /gene="pstC"
                     /locus_tag="VFR25_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9941376.1"
                     /translation="MRLPLPPRRRGLTMDGMYRGTLAAAAAAVFLIVALIVIEITHGS
                     LLSIHTLGWGFLVQTQWDPVADHYGALPYIYGTLVSSILALVLAVPLGLGSAVFLAEL
                     AKGRAAAIVSFVMELLAAIPSIVYGIWGFFVLAPFLRTLLEPWLIANFGYLPLFRGTP
                     FGIGMLNAGIVLAIMIVPTIVSISREVLLAIPRSLREASLALGATRAEAIGITLDAAK
                     PGILGSIVLALGRALGETMAVTMVIGNTNRISASIIDPAATMASVIANEFTEATGALY
                     LSALIQIALVLFAVTIVVNALARGLVWLTTGGRRDISAAT"
     gene            complement(1983..3050)
                     /gene="pstS"
                     /locus_tag="VFR25_09795"
     CDS             complement(1983..3050)
                     /gene="pstS"
                     /locus_tag="VFR25_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET9941377.1"
                     /translation="MRINRILAAALVASAIAGCSSGGGPAKKPLSNITLTGAGATFPY
                     PIYSKWFDMYHESTGVQINYQSIGSGGGIQQLKAGTVDFGASDAPLNDEKLGEMPHPV
                     VHIPTVAGAVVLAYNIPDLAQPLQLTPKALAGIFMGTVKTWNDPAIKAANPGVTLPAT
                     AVLAVHRSDGSGTSNIFTTYLSAVSPDWKSKVGANTSVAWPAGVGAKGNEGVTGQVKQ
                     TPGSIGYVELAYAKQNNLTVARLQNSSGAYIDPSLASTTAAIEASAQALAADVRASTV
                     NAAGPDAYPIAALTFILVYQDQADEAKAKALADFLTWAIHQGQDQVEALQYARLPAAI
                     VQVDEQEIARLTAAGKPLASP"
     gene            complement(3069..>3900)
                     /gene="pstS"
                     /locus_tag="VFR25_09800"
     CDS             complement(3069..>3900)
                     /gene="pstS"
                     /locus_tag="VFR25_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET9941378.1"
                     /translation="VDFGASDAALSNQRLKEMPRPVLHFPTAAGAVVLAYNVDGVSQP
                     LNLTSDIIAGIYLGKITQWNDKAIAAANPGVALPPSPILAVHRSDGSGTTNIFTTYLG
                     VVSRSWKELVGANTSVSWPVGIGGKGNEGVTGLVRQTPGSIGYVELAYAKQNHLAYAN
                     VRNASGKFIEPTLASTTAAAQGAAAALAKDVRFPIANSSNPDAYPIAGMTFLLVYKDQ
                     RDADKGKALADFIRWANSKGQEINETLEYARLPETVVKVNEATLMQLRVAGRPIASAQ
                     "
BASE COUNT          683 a         1306 c         1302 g          609 t
ORIGIN      
        1 gtgccccagt cgctcgagct cgtgctcgcg agtcgaaatc tggagcgcgt agcggacctg
       61 gccaccaacg tggccgaaga gacgatcttc gttgccgagg cgcgggtcat caagcaccac
      121 gccgacgaag aggcggataa gtctagcggt cgcgaacccg ccggacggta agccgaagca
      181 taagcgagat caaagtcacg aggagcacca ggaccagcgc cccggtccaa gcctgccgat
      241 tccagtccgt gtagggcgcc ttcgcgtagt catagatgaa gaccggcagg gtcgcgatcg
      301 gctgatccat gcggagcgac cagaaccggt tcccgagcgc ggtgaagagg agcggggctg
      361 tctcgcccgc cgcgcgcgcc acggccagca tgctcgccgt cacgatcccc gcccgcgcgg
      421 ccgggagcac gatctgttgc gtggtcttcc accgcggagc tcccaaggcg agcgcggcct
      481 ggcggtacga ctgaggcacg agccgcacca tctcctcggt ggcgcggatg atcggcggca
      541 gcatgagaat cgctagcgcc acgctccccg cgaagcccga gaaccgcccc atcggaatca
      601 ccacgagccc gtacgcgacc accccgacca cgatcgacgg aacgccgctc agcacgtcgg
      661 tcgtgtagcg gaccagcgtg gcgaaacgtc cgcgcccgta ctccgacagg tagacccccg
      721 ctgcgatccc gagcggcagc gcgatgaggg ccgcgacgcc taccacgaga agcgtcccga
      781 cgatcgcgtt cgccatgcct ccgcccgctt cgccgacggg cttcggcatg tgcgtgaaaa
      841 aagccagatt gaggcccgcc accccgttcg cgagcaagca gcccatgatg aggacgagcg
      901 gcagcacgac gacgcacgcc gcgagtacgc acgcgccgat cacgacccgg ttggtcacgg
      961 cacgcaagag gtgcaccgag cgcggagtgc gatccaccca gaccggcgtc accgctcccg
     1021 cttcggcggc cggcgtcatg ttgcggccga gatgtcgcgc cgcccgcccg tggtgagcca
     1081 gacgagtcca cgcgcgagcg cattcacgac gatggtcacg gcaaagagga cgagcgcgat
     1141 ctggatgagg gccgagaggt agagcgcccc cgtcgcctcg gtgaactcat tggcgatgac
     1201 gctcgccatg gtggcggcgg gatcgatgat ggaggccgag atccggttcg tgttcccgat
     1261 caccatcgtg accgccatcg tctcccccag cgcccgtccg agcgcgagca cgatcgagcc
     1321 caggatcccc ggcttcgccg cgtcgagtgt gattccaatc gcctccgccc gcgtggcacc
     1381 gagcgccagc gacgcctcgc ggagcgagcg ggggattgcg agcagcacct cgcgcgagat
     1441 cgagacgatc gtgggaacga tcatgattgc gagcacgatc ccagcgttca gcatgccgat
     1501 cccgaacggc gtgccccgga agagagggag atatccaaag ttcgcgatca gccacggctc
     1561 gagcaaggtt cgtagaaatg gagccagcac gaagaagccc cagattccgt acacgatgct
     1621 cggaatcgcc gcgagcagct ccatcacgaa cgatacgatg gcggccgccc gccccttcgc
     1681 caattcggcg aggaacaccg cgctcccaag cccgagcggc accgccaaca cgagcgccag
     1741 gatcgacgag acgagcgtgc cgtagatata cgggagcgct ccgtagtgat ctgcgacggg
     1801 atcccactgg gtctgaacga ggaagcccca gccgagcgtg tggatggaca ggagcgagcc
     1861 gtgcgtaatc tcgatgacga tgagagcgac gatgaggaac accgccgcgg cggctgcggc
     1921 cagcgtgccg cggtacatgc cgtccatcgt gaggccgcgc cgccgcggcg ggaggggaag
     1981 tctcacggcg aagccagggg cttcccggcc gccgtgaggc gagcgatctc ctgctcgtcc
     2041 acttgtacga tcgcggcggg cagtcgcgcg tactggagcg cttcgacttg atcctgaccc
     2101 tggtggatcg cccaggtgag gaagtcggcg agcgccttgg ccttcgcctc atccgcttga
     2161 tcctggtaga ccaggatgaa cgtgagcgcc gcgatcggat aggcgtcggg ccccgccgcg
     2221 ttgaccgtcg acgcacgcac gtctgccgcg agcgcttgcg cggacgcttc gatggccgcc
     2281 gtcgtcgagg cgagggacgg gtcgatgtac gcacccgacg aattctggag ccgtgcgacc
     2341 gtgaggttgt tctgtttcgc gtacgcgagc tcgacgtacc cgatcgatcc gggcgtctgc
     2401 ttcacctgcc ccgtgacgcc ctcgttccct ttggcaccga ctccggcggg ccaagccacc
     2461 gacgtgttcg ctcccacctt gctcttccag tcggggctga ccgccgagag atacgtggtg
     2521 aagatgttcg atgtgccgct tccgtcggac ctgtgcaccg cgagcaccgc ggtcgcggga
     2581 agggtcacgc cgggattggc cgccttgatc gcgggatcgt tccaggtctt gacggtgccc
     2641 atgaagatgc ccgccagagc cttgggagtg agctggagcg gctgcgcgag atcgggaatg
     2701 ttgtacgcga gcaccaccgc gcccgcgacg gtcgggatgt ggacgaccgg atgtggcatc
     2761 tcccccagct tctcgtcgtt gagcggcgcg tcgctcgcgc cgaagtcgac cgtgcccgcc
     2821 ttgagctgct ggatgccacc ccccgagccg atcgactggt agttgatctg gacccctgtc
     2881 gactcgtggt acatgtcgaa ccacttcgaa tagatcgggt acggaaaggt cgctcccgca
     2941 cccgtcagcg tgatgttgga gaggggcttc ttggccggcc cgcctccgct cgaacagcct
     3001 gcaatggccg aggcgacgag cgccgcggcc aggatccggt tgattctcat ggtttctctc
     3061 ccacagagct attgggccga ggcgatcgga cgccctgcca cgcgcagctg catcagggtc
     3121 gcttcgttca ccttcactac agtttcaggc aaacgagcgt actccaacgt ctcgttgatc
     3181 tcctgcccct tcgagttcgc ccagcggatg aaatccgcga gcgccttgcc cttgtccgcg
     3241 tcgcgctgat ccttgtagac gagcaggaac gtcatgcccg cgatcggata ggcatccgga
     3301 ttagaggagt tcgcgatcgg gaagcggacg tccttcgcga gcgcggccgc ggcgccctgg
     3361 gccgccgccg tcgtcgaggc gagcgtcggc tcgatgaact tgcccgacgc gttccgcaca
     3421 ttcgcgtaag cgaggtggtt ctgcttcgcg taggcgagct cgacgtagcc gatcgagccc
     3481 ggggtctgac ggaccagccc ggtcacgccc tcgttcccct tgccgccgat gccgacaggc
     3541 caggacacgg aggtgttcgc gccgaccagc tccttccacg agcggctcac caccccgagg
     3601 tacgtcgtga agatgttcgt cgtgccgcta ccgtccgagc ggtgcaccgc gaggatcggc
     3661 gaaggcggaa gggccacgcc gggattggcc gctgcgatcg ccttgtcgtt ccactgcgtg
     3721 atcttgccga ggtaaatccc ggcaatgatg tcggacgtga ggttgagcgg ctgggacacc
     3781 ccgtcgacgt tgtacgcgag cacgacggcg cccgccgcgg tcgggaagtg gagcaccggg
     3841 cgcggcatct ccttcaagcg ctggttcgag agcgcggcgt cgctcgcgcc gaagtcgacc
//
LOCUS       DASTKC010000234        17233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1622318, whole genome shotgun sequence.
ACCESSION   DASTKC010000234 DASTKC010000000
VERSION     DASTKC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17233
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622318"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..381)
                     /locus_tag="VFR25_09805"
     CDS             complement(28..381)
                     /locus_tag="VFR25_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941379.1"
                     /translation="MRIGALLLAALMAPASCGRVEPRRTVLLHMNGYAFNDSNPTLAF
                     TTRERVLFIVRNDETTMVRHNFRIAALGVRCDYELGPGEERAVAVTLPDTPGVFPYDC
                     CTHQGMGGTIVVKRR"
     gene            447..875
                     /locus_tag="VFR25_09810"
     CDS             447..875
                     /locus_tag="VFR25_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941380.1"
                     /translation="MILAQTARPPVTIAIESPRRRVARGDQLDLMLTLAVDPGFRIES
                     VDAPAGRARATSIQLGLADGLTPGPMTAPEDSVDPLGGGRVPAYVGRIAIRVPLTVEA
                     SCLPGPAPLAARVLFQATGERGLLPPDEVEVHAELDVTAG"
     gene            complement(907..1482)
                     /locus_tag="VFR25_09815"
     CDS             complement(907..1482)
                     /locus_tag="VFR25_09815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9941381.1"
                     /translation="MTAPTHDLTLITFYAYLAAFFLVERFLTRSTFGSTTRGDRDPLT
                     PLFFVVPAIGIFTWMFLQHGPLPPHPSWWSYGAGLLIGISGMAVRILGKRTLGRMFTV
                     RVQIQEDHKIVDTGMYARVRHPLYAGFILEWMAPPLILGSPIGFLLLTLPLAIVVLLR
                     IPREEALLIETFGDRYRAYMARTKRLIPGIW"
     gene            complement(1479..2288)
                     /locus_tag="VFR25_09820"
     CDS             complement(1479..2288)
                     /locus_tag="VFR25_09820"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HET9941382.1"
                     /translation="MCTLLIGIDVLGPGTAVLGANRDESPERATAGPGVLVASPRVVG
                     GRDLVARGTWLAIRDARFVTALMNRRPRPGDPTDGLRSRGLLCLEAASTARLDRPARI
                     DPETGEARPDAWDSALRSLGSGEYGRCTLVGIDPDLGSWAVVGGGGDEPHGAPIPVGW
                     HAITHAELDDPDEPRTRHVLSKLEGMKPRSADEALALFASILRSHGEDGGPAVCIHRE
                     HFPTVSSTLLALGAVDRPRYLHADGPPCVTEYQDHSTLLGADAPHEARERA"
     gene            complement(2305..3477)
                     /locus_tag="VFR25_09825"
     CDS             complement(2305..3477)
                     /locus_tag="VFR25_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9941383.1"
                     /translation="MSERTAPPPATAPAVPYVPGLDGVPAAKSSICFIDGDQGILEYR
                     GYPIETLAEHSTFEETSYLLLWGKLPARADLDKFTRDLASNRDVRFRLIDLLKCLPDT
                     GHPMEALQASIAALGMFYPDRDVDKPEDRYNSSIRIIAKATTLVAAYYRLSTGRDYIP
                     PKEDLSHASNFLYMLTGEDPEPDVARVMDVCFILHAEHSMNASTFAGRVTASTLADPY
                     TVVSSAIGALTGPLHGGANEQVIHMLQGIGEAEKARPYIEDLITRKQKIMGIGHRIYR
                     VKDPRALVLQKLAAQMIAKRGGDPLLLIAQEVEKVVAEHLGAKRIYPNVDFYSGVVYT
                     AMGLPPALFTPMFAVARISGWLAHWIEQLQGNRIYRPDQIYTGTHNEPYVPVEERV"
     gene            complement(4016..4999)
                     /locus_tag="VFR25_09830"
     CDS             complement(4016..4999)
                     /locus_tag="VFR25_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET9941384.1"
                     /translation="MSARMGALALLALLALPTPAGATFSIVARDPDTGEIGVAVQSHY
                     FAVGPIVPWAEPGIGAVATQSLVEVSYGPKGLELMRNGKSAEDALKELLDKDEHADVR
                     QVAMIDVHGNVANHTGQRCIADAGDKKGENYSCQANLMANDKVWGAMAQAFETSKGDL
                     AERLLKALEAGQAAGGDIRGQQSAAIVIVKGKRSNQPWADRVLDLRVDDNPKPIAELR
                     RLVTLWRAYRNVDQGDAYVADNKVEDAMKSYAEASRLAPGNNEILFWQAATLWKLDRE
                     KEATPIFKKVFARDRRWVALVPRLVPAGLLDDDKNDVKRIQALAPAAKRHR"
     gene            5272..6996
                     /locus_tag="VFR25_09835"
     CDS             5272..6996
                     /locus_tag="VFR25_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9941385.1"
                     /translation="MSHALRMKLLSNGAVIAMLCVFCVLAPTADASWAVNGNQASLTI
                     KNQVNAQMISDGAGGTFVVWQDFRTDTLADVFAQRFDMSGNRLWGSSGVAVCTVSDAQ
                     ESPVLCSNGSGGIIVAWQDRRSTTNYDIYAQALNGNGTLLWTPSTGVPICTFASNQIL
                     EVIATDNAGGAIIGWRDQRNGTLNADVFAQRVNGSGVTQWTLDGVAVCTAAGTQTDVR
                     ILPDGIGGAFLVWRDQRGGPFSDDIYAQSIDAIGTTRWTTNGVAVAVAAQTQVEPSIS
                     TDGKFGLLVAWQDTRNGTDADIFVQRIKPDGTPRWAANGIQVCDQDSTQQHALVATDG
                     LAGAIVAWIDQRVSSRNADIYAQRVDSTGAVAWAANGVAVCTADSAQSLSTIVPDKSG
                     GVILGWDDGRGAGNNFDIYAQAVRQNGTVRWATNGVPIASAANTRRLTASAPDGFGGA
                     LFAWEDNRLGSYDVFAYRISPVGTGVEPSTPAARAGLLAPRPNPFNPHTTIEFALDQP
                     SRFRLAIHDVQGRLVRVLAEGEAPAGNRSVAWDGSRADGTPCASGAYFAVLTLPAETR
                     TSSLRLIR"
     gene            7146..8354
                     /locus_tag="VFR25_09840"
     CDS             7146..8354
                     /locus_tag="VFR25_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HET9941386.1"
                     /translation="MDIFEKCRNWKEHRLAKAAGVYPYFLPISENHGTEVTIGGKRLI
                     MAGSNNYLGLTKHPKVMAAAEDALLRFGTSNNGSRFLNGTLELHEQLEERLAKFMKKE
                     AALVYSTGYLTNLGSISALADKGDVVILDKDAHACIVDGARLSYGDVKRFRHNDIRDL
                     ERVLSSAPPKSGKLVVVDGLYSMEGDLAPLPDIVRLARDYNARLLVDDAHGIGVLGAR
                     GAGACEAMNVEDQIDLVMGTFSKSFASLGGFIAGPRAVMDYLRHHARAMIFSASMTPA
                     SVAAALAALEIIQSEEGAGLRDRLRQISIKMRSGFRAQGLEVGSGVETPIVPIFIGDR
                     LKTMQVWKKILDRGVFLNAILVPAVAPGRDLLRTSYMATHQDDQLDTILKVVGEVHQE
                     VGLELVSKES"
     gene            8460..9482
                     /locus_tag="VFR25_09845"
     CDS             8460..9482
                     /locus_tag="VFR25_09845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9941387.1"
                     /translation="MPPLRMDERVRLTPGKNPFFEHGQAAYFLATEGGRVVGRVAAST
                     DQNHDSIHGERQVAFGFFEADTAEAAAALLERAAAWGKEHGAEVLRGPLSFTTNDECG
                     LLIEGFEYRPAIMMPHNPPEYAAWIEAAGLVKAKDLYTFRAPIPSESPPVFRRIASAA
                     RRMPGLVTRPVEMKRFDEELTRVKSVYNEAWEKNWGFVPMTDGEIDHMAKQLKPAIVP
                     ELVRFVEVDGAPIGFGLIIPDINIALRHLGGRLFPFGIVRLLLTLPRIREGRLMALGV
                     RSDWRRKGIGPLLVDDLILALRAKGYGSVEIGWTLETNDDVNSLIAGVGGVRTGIYRV
                     YERRLG"
     gene            9479..10468
                     /locus_tag="VFR25_09850"
     CDS             9479..10468
                     /locus_tag="VFR25_09850"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9941388.1"
                     /translation="MSEPLAVVTGASGFVGSHIVDELLARGARVRCLLRKSSSTRWLD
                     ESKVEIARVPLDHVEPMAEALQGATWIVHAAGLTRARSPKEFHEANVGATERMLRAAM
                     QAGSSLERFLLVSSQSVAGPAGPDGRPVTEGERPDPASVYGVSKLRAEELTLLLKDRL
                     PVTVIRPPAVYGPRDTAIFRVFAAVKRHVLPVLKKGARFTLVHAEDLARAVHLALVEP
                     KAKGQVYFVGEPEITDYVEVGTAVSRALGSRAMIVSPPGFLLGAGAIAAEVFGALTGT
                     APLLTREKLDEIRAGDWICSSAKIRHELGWEPQLLLNEGIKRTADWYRQAGWV"
     gene            10513..12999
                     /locus_tag="VFR25_09855"
     CDS             10513..12999
                     /locus_tag="VFR25_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HET9941389.1"
                     /translation="MITRRAAVLLAALASLALPASVCAYAVSKNVTLLGHLDNYAAYS
                     ACCSYVHSDGREYAVLGTTTGTSIVNITNPSLPYEVAFIPGLGSQWREMKQFRNYIYI
                     TTEAAGGGVQIVRMLDPEHPVLVKTYTGTFNQEHTVSVDSTRSLLILNGTRLNGLAAG
                     MRVLSLADPENPVEIGAYQTDYVHDSWVRNDTLFASGITTGTMHVVNFSDPTNPYDIT
                     DFTYPGARTHSSETSPNGRYLYVCDEMNYGTLKVFDIADVFFHPMVREMTVNPISIVH
                     NVHVRGETAYIAWYTEGVHLFDLHDPAMPVEWGWYDTYGSFSGGFHGVWEVSANFPSG
                     TFIASDIENGLFIFRATKNYGWVKLVVKNAAQSLLSGVDVTALGEADSTQTQGLGTAG
                     LALSPGTHMLRVKKYGYTTVNVPVTTSMGSHDSIAVTLPLAPTGTIAGTVRRDSDQTG
                     LPGATVEAEDTPKAATTAAAGTYSLTGMVPETMRLIADRPGYVPEERTVTLQPGASMT
                     QDFRLLRAAWYDSCDTDKGWSLSAAGDNATDGLWTRAIPVGSSVPGSGAAPRPRGPAA
                     LAAQHPEPGEGGYNAEGPVQPGDDYSPGTGYCFVTANGLAGGDPANGDVDAGRTTLTT
                     PPLNMSGMTEPTIVFRRWYYMNTPGEPDSFQCDISRDGVTWVNMLNTRLSHPEWHLEE
                     FRVRDYILPGSAVRVRFIAQDQGVGTIVEAAVDDFELFDAALHTTGVVEGPPPTPAAV
                     LGVPHPNPASHASSLSLTLKDAGQVEVAVYDVQGRRVAMLFHGAAPSGALELTWKGDD
                     AKGRPVASGVYWLRAAAGGETFTRRIVRAR"
     gene            complement(13018..13944)
                     /locus_tag="VFR25_09860"
     CDS             complement(13018..13944)
                     /locus_tag="VFR25_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9941390.1"
                     /translation="MKRTGHVPFMQLRIGIFVVIAVMLLLWATFQSGAFKLGKEEKIT
                     IGFNRIGGLEEGAIVRLNGVPVGVVRDIRLQPQSNHVAVDLGVKTGTRSRLHQGAGAS
                     ITTVGFLAELYVELIDPNPEAPPIHSDSEIRTDILADPAMIMNKANMIVDTLQTLVGN
                     LNQSGRAFAQGQGTLGRLSRDERLYEQMVALTKEATGLTERINANQARVTDRLVSLTG
                     SLDSLTWQLQHGNGTAAQLLRKGDLYDQLASSTARLDSVLATVQSGKGTFGQMMADTV
                     LYQDTKALMGSMKRLMAAIEKDPKKYFKFSLF"
     gene            complement(13941..14750)
                     /locus_tag="VFR25_09865"
     CDS             complement(13941..14750)
                     /locus_tag="VFR25_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9941391.1"
                     /translation="MIEFRDVDYSIDGRPILKGISLAVRAGETRVILGPSGSGKSTTL
                     RLLVGLVKPDKGSIFIDGEDIARVSEERLRQIRHGIGMVFQQGALFDSMTVGENVGYA
                     LLEQNEDEADVEARVREVLRRVGLDDELIDRMPDEMSGGMQRRVAIGRALAARDPKIL
                     LYDEPTTGLDPQSAELITDLIVQLRDTLGKTSILVTHDIADAYKVGNTITVLSEGRVV
                     FDGNAVDLDRSNDSFIHQFLAPFRTAVAAAAQRLTDAPRPASAPPLSGGTP"
     gene            complement(14755..15528)
                     /locus_tag="VFR25_09870"
     CDS             complement(14755..15528)
                     /locus_tag="VFR25_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9941392.1"
                     /translation="MIQALIERLRRTLEVVQDFSLFTAALLKTAFIPPFYGREMMHQL
                     YFAGIGSLLIVVVSGTVAGQALGLQLARELAQSGAKSQLGHFMVISVVRALGPVLVGM
                     VVASRMAAGITAEIGAMRSSDQIDALVAFGTDPMKRLVVPRMVALVIALPCLVIIGDA
                     LAVTGGGLVGLQYHITWESYWAGVLHYLTPRNILVGMIKPVLFAVMIVAVACWKGFTS
                     EGGAKGVGASTTESVVISSVGLLVLDGVLTRLIFRLLHW"
     gene            complement(15559..16212)
                     /locus_tag="VFR25_09875"
     CDS             complement(15559..16212)
                     /locus_tag="VFR25_09875"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9941393.1"
                     /translation="MKKSNYGNLPINHSPEPIRLFKSDFLEFFTHIHPGVVLLLFVPV
                     VVVFLVRAYGDFGGALLPVAIGYVGGLLLWSLAEYLLHRYVFHYEPEPEWLQRVWYLI
                     HGVHHEQPQCKTRLVMPPILSIPLALLHYGLFVLVVGVLFHAPHWVAPLFAGFITGYL
                     TYDMVHYATHHLAMKWGFLKFLKRYHLLHHYKTPDDRYGISSPIWDVVFGTRPPKDS"
     gene            16333..17010
                     /locus_tag="VFR25_09880"
     CDS             16333..17010
                     /locus_tag="VFR25_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET9941394.1"
                     /translation="MPPFRINEIFLSVQGEGIHAGERTVFVRFYGCPLRCVWCDQPEA
                     LAHTGVGRFTALSADEVRDQVLAFSSVRRVCLTGGEPMIAHREPLVRLLDELRGAGYW
                     ISVETSGSRVVEEALDRIDFWTIAPKGRSARTFAGDPVLAQLPTLKRLLELPEERRQL
                     KFVIQGEEDIEDSVRLLDSLGYRGSIVLQPEHGAGSANAIFAAWPWARYPDARLIPQT
                     HKSMGLH"
     gene            17007..>17233
                     /locus_tag="VFR25_09885"
     CDS             17007..>17233
                     /locus_tag="VFR25_09885"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET9941395.1"
                     /translation="MNVRLTHAFTFEAAHRLPHAPEGHKCRRLHGHSFRVEVTVEGPV
                     DPATGWFLDYGEIKEAAEPLRARLDHHYLNE"
BASE COUNT         2803 a         5823 c         5849 g         2758 t
ORIGIN      
        1 gccgcccgtc gcttccgagc cgccgcgcta gcggcgcttc accacgatgg tcccgcccat
       61 gccctggtgg gtacagcagt cgtaggggaa cacgccgggg gtgtccggca gcgtgaccgc
      121 gaccgcccgc tcctcccctg gcccgagctc gtagtcgcag cggacgccga gcgcggcgat
      181 ccggaagttg tgccggacca tcgtcgtctc gtcgttcctg acgatgaaga gaacgcgctc
      241 gcgggtcgta aacgcgagcg tgggattgga gtcgttgaag gcgtagccgt tcatgtggag
      301 gaggaccgtg cgcctaggct cgacgcggcc gcacgaagcc ggggccatga gcgccgcgag
      361 aagcaaggcg ccgatccgca ttcgtcgttt catcgcggag tagtgtacgc tcgcgtcatg
      421 aacctcgcgt cccgcgcgga gcgcgcgtga tcctcgccca gaccgcgcgc cctcccgtca
      481 ccatcgcgat cgaatccccg cgccgtcgcg tcgcgcgcgg agaccagctc gatctcatgc
      541 tgaccctcgc cgtcgatccc gggtttcgaa tcgagtccgt ggacgcgccg gcaggccggg
      601 cgcgggccac gtcgatccag ctcggactcg cggatggact cacgccggga ccgatgaccg
      661 cgcccgagga ctccgtcgat ccactcggcg gcggccgcgt gccggcgtac gtcggacgga
      721 tcgcgatccg ggtcccgctc acggtcgagg cgtcgtgcct tccaggcccc gcgcctctcg
      781 cggcgcgcgt cctcttccag gccacgggcg agcgcggcct ccttcctccc gatgaagtcg
      841 aggtgcacgc cgagctggac gtgaccgcgg gctgagtcag gcgccgctcg acctgcttcc
      901 aaccgcctac cagatcccgg gaatcagccg cttcgtgcgc gccatgtagg cgcggtaccg
      961 atctccgaac gtctcgatca ggagcgcctc ctcgcgcggg atgcggagca ggaccacgat
     1021 cgcgagcggg agggtgagga ggaggaatcc gatcgggctc ccgaggatga ggggcggcgc
     1081 catccattcg aggatgaagc ccgcatagag gggatgccgc acgcgggcgt acatccccgt
     1141 gtcgacgatc ttgtgatcct cctggatctg gacccgcacc gtgaacatgc gtcccagcgt
     1201 ccgcttgccc aggatgcgca ccgccatgcc ggagatcccg atgagaagtc ccgcgccgta
     1261 ggaccaccac gacgggtgcg gggggagcgg tccgtgctgc aggaacatcc aggtgaagat
     1321 cccgatcgcc ggaaccacga agaagagggg cgtgagcgga tcgcgatcgc cgcgcgtcgt
     1381 gctcccgaac gtcgatcgcg tgaggaagcg ctccacgagg aagaaggccg ccaggtaggc
     1441 atagaacgtg atcagcgtga gatcgtgggt cggagcggtc atgcgcgttc cctcgcttcg
     1501 tggggcgcgt ccgcgccgag gagcgtggag tgatcttgat attcggtgac gcacggaggg
     1561 ccgtccgcgt gaagatatcg cggccggtcc acggcgccga gcgcgaggag cgtgctcgat
     1621 acggtgggga agtgctcccg gtggatgcag acggccggcc cgccgtcctc gccgtggctc
     1681 cggagaatcg aggcgaagag cgcgagcgcc tcgtccgcgc tccgcggctt catcccttcg
     1741 agcttcgaga ggacgtggcg ggtgcgtggc tcgtccggat cgtccagctc ggcgtgcgtg
     1801 atcgcgtgcc atcccaccgg gatcggagcg ccgtggggct cgtcaccgcc gccacccacc
     1861 accgcccacg acccgagatc gggatcgatg ccgacgagcg tgcagcgccc gtattcgccg
     1921 cttccgaggg accgcagggc cgagtcccac gcgtcggggc gcgcttcgcc ggtctcggga
     1981 tcgatgcgcg ccggccgatc gagccgcgcc gtcgaagccg cttcgagaca gaggaggccg
     2041 cgcgatcgga gcccgtccgt gggatcgcct gggcggggcc gccggttcat gagcgcggtc
     2101 acgaagcgtg cgtcgcgaat cgcgagccac gtccctcggg cgacgagatc gcggccgccg
     2161 accacgcgcg gcgaagcgac gagcacgccc ggtccggcgg tggcgcgctc gggagactcg
     2221 tcccgattcg cgcccaggac cgcggtcccc ggtccgagga cgtcgattcc gatgaggagc
     2281 gtgcacacgg cgcggggcgc cgggctagac ccgctcctcg accgggacgt agggctcgtt
     2341 gtgggtgccg gtgtagatct ggtcggggcg gtagatgcga ttcccctgga gctgctcgat
     2401 ccagtgcgcg agccagccgg agatgcgcgc gaccgcgaac atcggggtga agagcgcggg
     2461 cggaaggccc atcgccgtgt acaccacgcc cgagtagaag tcgacgttgg gatagattcg
     2521 cttcgcgccg aggtgctccg ccaccacctt ctcgacctcc tgcgcgatca ggagaagcgg
     2581 atcgccgccg cgcttcgcga tcatctgggc cgcgagcttc tggaggacga gcgcgcgcgg
     2641 atccttcacg cggtagatgc ggtgtccgat ccccatgatc ttctgcttcc gggtgatcag
     2701 gtcctcgatg tacggccgtg ccttctcggc ctccccgatc ccctggagca tgtggatcac
     2761 ctgctcgttc gcgccgccgt ggagcggccc cgtgagcgcg ccgatcgcgg aggagaccac
     2821 cgtgtacggg tcggcgagcg tggaggcggt gacgcgcccc gcgaacgtcg aggcgttcat
     2881 cgagtgctcc gcgtggagga tgaagcagac gtccatcacg cgcgcgacgt ccggctccgg
     2941 gtcctcgccg gtcagcatgt agaggaagtt cgacgcgtgc gagaggtctt ccttgggcgg
     3001 gatgtagtcg cgtcccgtgg agaggcggta gtacgcggcg acgagcgtcg tcgctttcgc
     3061 gatgatgcgg atcgacgagt tgtaccggtc ctccggcttg tccacgtcgc ggtcgggata
     3121 gaacatcccg agcgccgcga tcgaggcctg gagcgcctcc atcggatggc cggtgtcggg
     3181 caggcacttg aggagatcga tgagccggaa gcgcacatcc cggttggacg ccaggtcgcg
     3241 cgtgaacttg tcgaggtcgg cgcgcgcggg gagcttgccc cagaggagaa ggtacgacgt
     3301 ctcctcgaag gtgctgtgct ccgccagggt ctcgatgggg tagccccggt actcgaggat
     3361 gccctggtct ccgtcgatga agcagatgct cgacttcgcg gccgggaccc cgtccaggcc
     3421 cggaacgtag ggcacggccg gtgcggtggc ggggggcggc gcggtgcgct ccgacatggg
     3481 tttcctccgg gtgctcggct acgacgcggc gaacgaacgc atcattctgt cggtttatcc
     3541 attttgatgc aacgcctgcg cgggtggttt ggccggcgtg gggagggtgt gagggtccgg
     3601 aacaagcacg tcccggcgct cgctacctca ccgctgcggt gacttggctg gcgccgggcg
     3661 cgaaagttcc ggaccctcac accctccccg cgcctcccaa atcgacagag cacggggcac
     3721 accggaatgg cagcgcgcga agcgaccggc gctcgcccaa acgggtggcg cctatcgagg
     3781 ggccaccaca cgccggagtg aggattgccg ctcgagagcc cccgacatcg agacgctcac
     3841 gccggcgaag cacttcgcgt tcttcggcgt catgggaggc gaggcgagtg tgtggggggc
     3901 cggaactttc gcgcccggcg ccaaccaagt caccccaggg gtgcgatagc gagcgccggg
     3961 acgtgcttgt tcaggccccc cacacactca cctcgcccgg ccgagtcgct aagagctacc
     4021 ggtgtcgctt cgccgcaggc gcaagcgcct ggatgcgctt cacatcgttc ttgtcgtcgt
     4081 cgagaagccc cgcgggaacg agccgtggaa cgagcgcgac ccaccggcgg tcccgggcga
     4141 acaccttctt gaagatcggc gtcgcctcct tttcccggtc gagcttccag agggtggccg
     4201 cctgccagaa caggatctcg ttgtttccgg gggcgagccg ggacgcttcc gcgtaggact
     4261 tcatggcgtc ctcgaccttg ttgtcggcca cgtaggcgtc gccctggtcc acgttccggt
     4321 acgcgcgcca caaggtcacg aggcgcctga gctccgcgat cggcttcgga ttgtcgtcca
     4381 cgcgaaggtc gaggacgcgg tcggcccagg gctggttcga tcgcttcccc ttcacgatca
     4441 cgatggccgc cgactgctgg ccgcgaatat cgcctccggc ggcctgaccc gcttcgagcg
     4501 ccttcaagag acgctccgcg aggtccccct tcgaggtctc gaaggcctgg gccatcgcgc
     4561 cccagacctt gtcgttcgcc atgagattgg cctggcagga atagttctcc cccttcttgt
     4621 ccccggcgtc ggcgatgcag cgttggcccg tgtggttcgc cacgttcccg tggacatcga
     4681 tcatcgcgac ctggcgcacg tcggcgtgct cgtccttgtc gaggagctcc ttgagcgcgt
     4741 cctcggcgga cttcccattg cgcatgagct cgagcccctt cgggccatag ctcacttcga
     4801 cgagggattg cgtggcgacg gcgccaatcc ccggctcggc ccagggcacg atcggtccga
     4861 ccgcgaagta gtgcgattgg acggccactc cgatctcacc cgtgtccggg tcgcgcgcga
     4921 cgatcgagaa ggtggctccc gcgggcgtgg gaagcgcgag aagcgcgagc agtgcgaggg
     4981 cgcccatccg tgcgctcatg aagatcctcc ctggacgtgt gtgcggacgc gggcggccgg
     5041 taacctccgt ccgtgtggca atttccccaa aacgattcca gcccttccgc caaaccaccc
     5101 agccccgcgg ctggacccag ccacgggccc gtatggggaa taagccccat ggctgtaacc
     5161 gattgccctg gcgctagttg tggcgagctt tccacatcgt ttacggtgag gcgcggctcc
     5221 tgcatactag tcgccgcaac cgtgacccgc aagcgctcga cctccaacct catgagccac
     5281 gccctgcgca tgaaactcct ttcgaatggc gccgtcatcg ccatgctctg tgtcttctgc
     5341 gtgctcgctc cgaccgccga tgcgtcctgg gccgtcaacg ggaaccaggc gtccctcacc
     5401 atcaagaacc aggtgaacgc gcagatgatc tcggacggcg ccggcggaac gttcgtcgtc
     5461 tggcaggact tccgcaccga cacgctggcc gacgtcttcg cgcagcggtt cgacatgagc
     5521 ggcaaccggc tgtggggaag cagcggggtc gccgtctgca ccgtgtccga cgcgcaggaa
     5581 tcgccggtcc tctgcagcaa tggatcgggg gggatcatcg tcgcgtggca ggaccggcgc
     5641 agcacgacca attacgacat ctacgcgcaa gcgttgaacg gaaacgggac gcttctctgg
     5701 acgccttcga cgggcgtgcc catctgcacc tttgcatcca accagattct cgaagtcatc
     5761 gcgacggaca acgcgggtgg cgcgatcatc ggctggaggg accaaagaaa cggaacgctg
     5821 aacgccgacg tcttcgcgca gcgcgtgaac ggctccggcg tgacccagtg gaccctcgat
     5881 ggcgtcgcgg tctgcacggc ggccggaacc cagaccgacg tccgcatcct gcccgacggc
     5941 atcggcggtg cgttcctcgt ctggcgggac cagaggggcg gacccttctc cgatgacatc
     6001 tacgcccaat ccatcgatgc catcgggacc acgcgctgga cgacgaacgg cgtggcggtg
     6061 gcggtcgctg cccagaccca agtggagccg tccatctcca cggacggcaa gttcggactc
     6121 ctcgtcgcgt ggcaggacac ccggaacggc accgacgcgg acatcttcgt ccagcgcatc
     6181 aagccggacg gcacgccgcg ctgggccgcg aacgggatcc aggtgtgcga tcaggacagc
     6241 acccagcagc acgcgctcgt cgcgacggac ggcctcgccg gcgccatcgt ggcctggatc
     6301 gatcagcgcg tcagctcgag gaacgccgat atctacgctc agagggtcga ctcgacgggc
     6361 gccgtcgcgt gggccgcgaa cggagtcgcc gtctgcaccg cggacagcgc acagtctctg
     6421 tccacgatcg tgcccgacaa gtcgggcggc gtgatcctgg gttgggatga cgggcgtggc
     6481 gccgggaaca atttcgacat ctacgcgcag gcggtgaggc agaacggtac ggtccgctgg
     6541 gccacgaacg gtgtcccgat cgcttccgcg gccaacacgc gccgcctcac ggcctcggct
     6601 ccggacggct tcggcggcgc tctcttcgcg tgggaagaca accggctggg ctcctatgac
     6661 gtcttcgcct accgcatctc gccggtggga acgggcgtgg agccttccac gccggccgcc
     6721 agggcgggcc ttctcgcgcc gcggccgaat cccttcaatc cgcacacgac catcgagttc
     6781 gcgctcgacc agccttcccg cttccggctc gccatccacg atgtccaggg ccggctcgtc
     6841 cgggtcctgg ccgagggcga agcccccgct gggaaccgct cggtggcgtg ggacggctcc
     6901 cgcgcggacg gcacgccctg cgcttcgggc gcctacttcg cggtcctcac ccttccggcg
     6961 gagacccgta cctcctcgct ccggctgatc cggtagctcc gggcgcaagc ccagtcacac
     7021 caacgagctg actccgcgga cgcccctccg gattgactcc gaggggcgaa attgagtatc
     7081 gtccgccgaa ctcctctgac ggcgccgcca agcggccgga ccccatccca aacgacagga
     7141 agcgcgtgga catcttcgag aagtgtcgca actggaagga gcaccgactg gccaaggcgg
     7201 ccggggtcta cccctacttc ctgccgatca gcgagaacca cggcacggag gtcacgatcg
     7261 gggggaagcg cctcatcatg gccgggtcca acaactatct cggtctgacc aagcacccca
     7321 aggtgatggc ggcggccgag gatgctcttc tccgcttcgg cacctcgaac aacggctccc
     7381 gtttcctcaa cggcacgctc gagctccacg agcagctcga ggagcgcctc gccaagttca
     7441 tgaagaagga agcggccctc gtctactcca cgggctacct gaccaacttg ggcagcatct
     7501 cggcgctggc ggacaagggc gacgtggtga tcctcgacaa ggatgcgcac gcctgcatcg
     7561 tggacggcgc gcggctctcc tacggcgatg tgaagcgatt ccgtcacaat gatatccgtg
     7621 acctcgagcg ggtcctctcc agcgccccgc ccaagtccgg gaagctcgtc gtggtcgacg
     7681 gtctctacag catggaaggc gacctcgccc cgctccccga catcgtccgg ctcgcccgcg
     7741 actacaacgc gcggctcctg gtggacgacg cgcacggcat cggcgtgctc ggcgcccgcg
     7801 gcgcaggcgc gtgcgaggcg atgaacgtcg aggaccagat cgatctcgtc atggggacct
     7861 tcagcaagtc gttcgcctcg ctgggcggct tcattgccgg tcccagggcg gtgatggact
     7921 atctgcgcca ccacgcgcgc gccatgatct tcagcgccag catgaccccc gcgagtgtcg
     7981 cggccgcgct ggccgcgctc gagatcatcc agtccgagga aggggccggg cttcgcgacc
     8041 gcctgcgcca gatctcgatc aagatgcgct ccggcttccg cgcccaaggc ctcgaggtcg
     8101 ggtcgggcgt cgagactccg atcgtgccga tcttcatcgg cgaccggctc aagacgatgc
     8161 aggtctggaa gaagatcctc gatcgcggcg tgttcctgaa cgcgatcctg gttccggccg
     8221 tggcgccggg gcgggatctc ctccgcacgt cctacatggc gacccaccag gacgaccagc
     8281 tcgacacgat cctcaaggtc gtgggcgagg tgcatcaaga ggtcggcctc gagctcgtct
     8341 ccaaggaaag ctagaaccgc cggaggcatg cgctggacct gatttcggtt cagggcgccg
     8401 cggacctcgc ccgcttcatc gaggttccct acaagcgcta ccggagcgac ccctactggg
     8461 tgccgccgct ccggatggac gagcgcgtcc ggctcacgcc gggcaagaat cccttcttcg
     8521 agcacggcca ggccgcctac ttcctcgcga cggaaggcgg tcgtgtggtc ggccgcgtcg
     8581 cggcttccac cgatcagaat cacgactcca tccatggtga gcggcaggtc gcgttcggat
     8641 tcttcgaagc ggacaccgcc gaggcggcgg cggcgctcct cgagcgcgcg gcggcgtggg
     8701 gcaaggagca cggggccgaa gtgcttcgcg gtcccttgag cttcacgacc aacgacgagt
     8761 gcggcctcct catcgagggc ttcgagtacc gccctgcgat catgatgccg cacaatcccc
     8821 ccgagtacgc agcctggatc gaagccgcgg gcctcgtgaa agccaaggac ctgtatacct
     8881 tccgcgcccc aatccccagc gagagcccgc cggtgttccg ccggatcgcc tcggccgccc
     8941 gccggatgcc cggactcgtg acgcgtccgg tcgagatgaa gcgcttcgac gaggagctca
     9001 cgcgggtcaa gagcgtctac aacgaggcgt gggagaagaa ctggggcttc gtccccatga
     9061 cggacgggga gatcgatcac atggcgaagc agctcaagcc cgcgatcgtc cccgagctgg
     9121 tccgcttcgt cgaggtggac ggcgcgccca tcggtttcgg gctcatcatc cccgacatca
     9181 acatcgcact tcggcatctc gggggccgac tcttcccgtt cgggatcgtg cggctcctcc
     9241 tgactctccc tcggatccgg gagggaaggc tcatggcgct cggcgtccga tcggactgga
     9301 gacggaaggg aatcgggccg ctcctcgtgg acgatctgat cctcgcgctt cgtgccaagg
     9361 gctacggaag cgtcgagatc ggctggactc tcgagaccaa cgacgacgtg aacagcctga
     9421 tcgccggagt aggcggcgtg cgcacgggga tctaccgggt ctacgagcgc cgcctcgggt
     9481 gagcgagcca ctcgcggtcg tcaccggagc gtccggcttc gtcgggagcc acatcgtcga
     9541 cgagctcctc gcgcgcggcg cgcgcgtgcg ctgcctgctc aggaagagct cctcgacccg
     9601 gtggctcgac gaatccaagg tggagatcgc acgcgtgccg ctcgaccacg tcgaacccat
     9661 ggccgaggcg cttcagggcg cgacgtggat cgtgcacgcg gccggcctca cccgcgcgcg
     9721 gagccccaag gaattccacg aggccaatgt cggcgcgacc gagcggatgc tccgtgcggc
     9781 gatgcaggcc gggagctcgc tcgagcgctt cctcctcgtg tcgagccagt cggtggccgg
     9841 ccccgccggt cctgacggcc gccccgtcac ggaaggggaa cgccccgatc ccgcgtccgt
     9901 ctatggcgtg tcgaagctcc gcgccgagga gctgacgctc ctcctcaagg atcgcctccc
     9961 cgtaacggtg atccgtcctc ccgcggtcta cggtccacgc gacaccgcca tctttcgcgt
    10021 cttcgccgcg gtcaagcggc acgtgctccc ggtgctcaaa aagggagccc gcttcactct
    10081 cgtccacgcc gaggatctcg cgcgcgcggt gcatctcgcg ctcgtcgagc cgaaggcgaa
    10141 gggacaggtc tacttcgtcg gcgagcccga gatcaccgac tacgtcgagg tcggcaccgc
    10201 ggtcagccgc gcgctcggat cgcgggccat gatcgtctcg cctccgggct tccttctcgg
    10261 agcgggcgcg atcgcggcgg aggtattcgg agccctcacc gggacggcgc cgctcctcac
    10321 acgggagaag ctggacgaga tccgcgccgg cgattggatc tgctcttccg ccaagatccg
    10381 ccatgagctc ggctgggagc cgcagctcct cctgaacgag ggaatcaagc gcacggccga
    10441 ctggtaccgt caagcgggtt gggtgtagac tccgatgtct ccgggccgcc gggcccgcgc
    10501 cgaggttcgc ccatgattac gcgccgcgcc gcagttctcc tcgccgcact cgcatcgctc
    10561 gcgcttcccg cgtccgtctg cgcctacgcc gtgtcgaaga acgtcacgct cctcgggcac
    10621 ctggacaact acgcggccta ctccgcctgc tgcagctacg tgcattcgga cgggagggag
    10681 tacgcggtcc tcggcaccac gacggggacc tcgatcgtga acatcacgaa tccgtccctt
    10741 ccttacgagg tcgcgttcat tcccgggctc ggttcgcaat ggcgcgagat gaagcagttt
    10801 cggaactaca tctacatcac gaccgaggcg gccggcggcg gcgtccagat cgtccggatg
    10861 ctcgatccgg agcatcccgt gctggtcaag acctacacgg ggacgttcaa tcaggagcac
    10921 acggtttcgg tggactcgac ccgctcgctg ctcatcctga acgggacgcg gttgaacgga
    10981 ctcgcggcgg gaatgcgcgt cctgtcgctc gcggatcccg agaacccggt cgagatcggg
    11041 gcgtaccaga cggactatgt gcacgatagc tgggtccgga acgacaccct cttcgcgagc
    11101 gggatcacca ccgggaccat gcacgtggtc aacttctccg accccacgaa cccctatgac
    11161 atcacggact tcacgtatcc cggggcgcgg acgcacagct ccgagacgtc gccgaacggg
    11221 cgctacctct acgtctgcga cgagatgaac tacgggacgc tcaaggtctt cgacatcgcg
    11281 gacgtcttct tccatccgat ggtccgggag atgaccgtga atccgatctc catcgtgcac
    11341 aacgtgcacg tgagggggga gaccgcctac atcgcctggt acacggaggg cgtgcacctc
    11401 ttcgaccttc acgatccggc catgccggtc gagtggggct ggtacgacac gtacgggagc
    11461 ttctccggcg gattccacgg cgtgtgggag gtgtcggcga acttcccgtc cgggacgttc
    11521 atcgcgagcg acatcgagaa cggccttttc atcttccgtg cgaccaagaa ctacggctgg
    11581 gtgaagctgg tcgtgaagaa tgcggcgcag tcgctcctct cgggcgtgga cgtcacggcg
    11641 ctgggagagg cggacagcac gcaaacacag ggcctgggca ccgccgggct cgcgctcagc
    11701 ccgggaaccc acatgctgcg cgtgaagaag tacggctaca cgaccgtcaa cgtgccggtg
    11761 accacgagca tggggagcca cgattcgatc gcggtgacgc tccccctggc tccgaccggc
    11821 acgatcgccg gaaccgtgcg ccgggactcg gatcagacgg gacttcccgg cgcgacggtg
    11881 gaggccgagg acacgccgaa ggccgcgacg accgcggccg ccggaacgta ttcgctcacc
    11941 gggatggtgc cggagaccat gcggctcatc gccgaccggc ccggctatgt gccggaagag
    12001 cggaccgtca ccctgcagcc gggcgcatcg atgacgcagg acttccgtct cctgcgcgcg
    12061 gcctggtacg actcgtgtga caccgacaag ggctggtccc tgagcgcggc aggggacaac
    12121 gcgaccgacg ggctctggac gcgcgccatt ccggtcggct cgagcgtgcc gggctcgggc
    12181 gcggcgccga ggccgcgcgg gcccgccgcg ctcgcggcgc agcaccccga gccgggcgaa
    12241 gggggctaca acgccgaggg cccggtgcag ccgggcgacg actactcgcc aggcacgggc
    12301 tattgcttcg tgaccgcgaa cggcctcgcg ggcggagatc ccgcgaacgg cgacgtggac
    12361 gccgggcgca ccacgctcac gaccccgccc ctcaacatgt ccggcatgac cgaaccgacg
    12421 atcgtgttcc gccgctggta ctacatgaac actccgggcg agcccgactc cttccagtgc
    12481 gacatctcgc gggacggcgt cacgtgggtg aacatgctca acacgcgtct cagccatccc
    12541 gagtggcatc tggaagaatt ccgggtgcgg gactacatcc tgcccgggag cgcggtgcgc
    12601 gtccggttca tcgcgcagga tcagggggtg ggcacgatcg tggaggccgc ggtcgacgac
    12661 ttcgagctct tcgacgcggc gctccatacg accggtgtcg tcgaggggcc gcccccgacg
    12721 ccggccgccg tgctcggcgt gccgcatccg aacccggcgt cgcatgcttc ctcgctctcg
    12781 ctcacgctca aggacgcagg gcaggtcgag gtcgcggtct acgacgtgca gggtcggcgg
    12841 gtcgcgatgc tcttccacgg cgccgctcca tccggagctc tcgagctcac gtggaagggg
    12901 gacgacgcca aggggcgtcc cgtggcgagc ggcgtctact ggctccgcgc cgcggctgga
    12961 ggcgagacct tcacgcggcg gatcgtgcgg gcgcgctagc gcgcccgggg cggcgggcta
    13021 gaacaagctg aacttgaagt acttcttggg atccttctcg atcgccgcca tgagccgctt
    13081 catcgatccc atcagcgcct tcgtgtcctg gtagagcacc gtgtcggcca tcatctggcc
    13141 gaacgtcccc ttgcccgact ggaccgtggc gaggacggaa tcgagccgcg cggtgctcga
    13201 ggcgagctgg tcgtagagat cacccttccg gagcaattgc gcggcggttc cgttcccgtg
    13261 ctggagctgc caggtgaggc tgtcgagcga tcccgtgagc gagaccagcc ggtcggtgac
    13321 gcgcgcctgg ttggcgttga tcctctccgt cagcccggtc gcctccttcg tgagcgccac
    13381 catctgctcg tagagccgct cgtcgcggct caagcgtccg agcgttccct gtccctgggc
    13441 gaatgcgcgc cccgactggt tcaagttccc gaccagggtc tggagcgtgt cgacgatcat
    13501 gttcgccttg ttcatgatca tggccgggtc ggccaggatg tcggtccgaa tttcggaatc
    13561 ggagtggatc gggggtgcct ccgggttcgg atcgatcagc tcgacgtaga gctcggccag
    13621 gaagcccacg gtcgtgatgc tggcgccagc gccctggtgg agcctcgagc gcgtccccgt
    13681 cttgaccccg aggtccaccg ccacgtggtt cgactggggc tggagcctga tgtcccgcac
    13741 cacgccgacc ggcacgccgt tcagtcgaac gatcgctccc tcctcgagac cgccgatccg
    13801 attgaagcca atcgtgatct tctcctcctt gccgagcttg aaggcgcccg attggaaggt
    13861 cgcccaaagg aggagcatga cggcgatcac gacgaagatg ccgatccgaa gctgcatgaa
    13921 ggggacgtgt ccggttcgct tcacggcgtt cctccactaa gtggcggcgc cgaggcaggc
    13981 cggggcgcgt cggtgaggcg ctgcgcggcc gccgcgaccg cggtgcggaa gggcgccaga
    14041 aactggtgga tgaaggaatc gttcgaccgg tcgagatcga cggcgttccc gtcgaagacg
    14101 acgcgtccct cgctcagaac cgtgatcgtg ttcccgacct tgtacgcgtc ggcgatgtcg
    14161 tgcgtgacca ggatcgacgt cttgccgagg gtgtcccgga gctggacgat gagatccgtg
    14221 atgagctcgg cgctctgcgg atcgaggccg gtcgtcggct cgtcgtagag gaggatcttc
    14281 ggatcccgcg cggccagggc gcgcccgatc gccacccgcc gctgcatccc gcccgacatc
    14341 tcgtccggca tgcggtcgat gagctcgtcg tcgaggccga cccggcggag gacctcgcgc
    14401 acgcgcgcct ccacgtcggc ctcgtcctcg ttctgctcga gaagcgcata gcccacgttc
    14461 tctcccaccg tcatcgaatc gaagagcgcc ccctgctgga acaccatgcc gatcccgtgg
    14521 cgaatctgcc ggagccgctc ctcggatacc ctcgcgatgt cctcgccatc gatgaagatg
    14581 cttcccttgt ccggcttgac gagcccgacc aggagccgga gcgtcgtgct cttgcccgac
    14641 cccgaagggc ccaggatgac gcgcgtttcc ccggcccgca cggccaggga gatgcccttc
    14701 aagatgggcc gtccgtcgat cgagtagtcc acgtctcgaa attcgatcat ccgatcacca
    14761 gtggagaagc cggaagatga gccgggtcag gactccgtcc aggaccagga gcccgaccga
    14821 ggagatgacc accgactccg tggtggacgc accgaccccc ttcgcccctc cctccgaggt
    14881 gaaacccttc cagcaggcca cggccacgat catgaccgcg aacaggacgg gcttgatcat
    14941 gccgacgagg atgttgcgcg gcgtgaggta gtggagcacc cccgcccagt acgattccca
    15001 ggtgatatgg tactgcaatc cgacgaggcc gcctccggtg acggcgaggg cgtcgccgat
    15061 gatgacgagg caggggagcg cgatcacgag cgccaccatg cggggcacga cgaggcgctt
    15121 catgggatcg gtgccgaagg cgaccagggc gtcgatctgg tcgctcgacc gcatcgctcc
    15181 gatctcggcc gtgatccccg ctgccatgcg ggacgccacc accatcccca cgagcaccgg
    15241 cccgagcgcg cgcaccaccg agatcaccat gaagtggccg agctggctct tcgcgcccga
    15301 ctgggccagc tcgcgcgcga gctgcagtcc gagcgcctgc cccgcgaccg tgcccgagac
    15361 caccacgatc aagagcgatc cgatcccggc gaagtagagc tggtgcatca tctcgcgccc
    15421 gtagaacggg gggatgaatg ccgtcttgag gagcgctgcc gtgaagagcg agaagtcctg
    15481 gacgacttcg agcgttctcc ggaggcgctc gatcagcgct tggatcatgc gaggggggcc
    15541 gggaggtccg gcgtgacctc aggagtcctt gggcggacgt gttccgaaca ccacgtccca
    15601 gatgggagag ctgattccgt agcggtcgtc gggggtcttg tagtggtgga gcaggtgata
    15661 gcgcttgagg aacttgagga agccccactt catggcgagg tggtgggtcg cgtagtggac
    15721 catgtcgtac gtgagatagc cggtgatgaa cccggcgaag agcggcgcca cccagtgcgg
    15781 cgcgtggaag agcacgccga ccacgaggac gaagagcccg tagtggagga gcgcgagcgg
    15841 gatgctgagg atcgggggca tcacgagccg cgtcttgcat tgcggctgct cgtggtgcac
    15901 gccgtggatc aggtaccaga cgcgctgaag ccactcgggt tccggctcgt agtggaacac
    15961 gtagcgatgg agcaggtact cggcgaggct ccagagaagg agacccccca cgtatccgat
    16021 cgccaccgga aggagcgccc ctccgaagtc cccgtaagcg cgcacgagga agaccaccac
    16081 gacgggcacg aagaggagga gcaccacgcc gggatggatg tgcgtgaaga actcgaggaa
    16141 gtcggatttg aagagccgga tcggctcggg gctgtgattg atcggcaggt tgccgtaatt
    16201 cgacttcttc atgaagtccc ttggtcgatc gggttggcgg gcgccacggc tccctcggga
    16261 cgccggaagc ccatgataag ggcacctttg agggaggtca accgacccgg ctcggtgcgg
    16321 tacactaccc ggatgccccc gtttcgaatc aacgagatct ttttgtcggt gcagggcgag
    16381 ggaatccacg ccggggagcg gaccgtgttc gtccgcttct acggatgccc gctccggtgc
    16441 gtctggtgcg accagccgga ggcgctcgcg cataccggcg tggggcgctt cacggccctt
    16501 tcggcggacg aggtgcggga ccaagtcctg gccttctcaa gtgttcgccg ggtctgcctg
    16561 accggggggg agcccatgat cgcccaccgg gagccgctcg tgcggctcct cgacgagctc
    16621 cgaggcgcgg gctactggat ctcggtcgag acgagcggct cgagggtcgt ggaggaagcg
    16681 ctcgatcgca tcgacttctg gaccatcgcg ccgaaggggc ggagcgcgcg gacgttcgcc
    16741 ggcgatcccg tcctggcgca gctccccacg ctgaagcgtt tgctcgagct cccggaggag
    16801 cggcgccagc tcaagttcgt gatccagggc gaggaggaca tcgaggattc ggtgcggctg
    16861 ctcgactcgc tcggctatcg cggttcgatc gtgctccagc cggaacacgg cgccggaagc
    16921 gccaacgcaa tcttcgcggc gtggccgtgg gcgcgctatc cggacgcgcg cctcatcccg
    16981 cagacgcaca agtccatggg gctccattga acgtccggct cacgcacgca ttcacgttcg
    17041 aggcggcgca tcgcctgccc cacgctcccg aagggcacaa gtgccgccgt ctccacgggc
    17101 attcgttccg ggtcgaggtg acggtggaag ggccggtcga cccggcgacg ggatggttcc
    17161 tcgattacgg ggagatcaag gaagcggccg agcccttgcg cgcgcggctc gaccatcact
    17221 acctgaacga gat
//
LOCUS       DASTKC010000235         7424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_204770, whole genome shotgun sequence.
ACCESSION   DASTKC010000235 DASTKC010000000
VERSION     DASTKC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204770"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VFR25_09890"
     CDS             <1..965
                     /locus_tag="VFR25_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET9941396.1"
                     /translation="PQDGRFRINVGGRDLDVRVSTFPTIHGENVVLRLLDRSAALIGM
                     AELGLGPTPLELLARMIDRPNGMVLVTGPTGSGKTTTLYACLHRIQGVERNIVTLEDP
                     VEIHLPSVRQTQVDPDVGLTFARGLRALLRQDPDVIMVGEIRDAETAEISVRSALTGH
                     LLLATLHTNDAAGAIPRLLDMDTEPFLLSSALAGVVAQRLVRRVCDRCKMTADVPRNL
                     REELGLPADARFLRGKGCVACGHGGYKGRVGVFEVLEMTDAIRERIHTRASGEEIARA
                     ARDAGMATLKDDALRKAALGLTTLDEALRVTAGDSARARAGVAR"
     gene            972..2186
                     /locus_tag="VFR25_09895"
     CDS             972..2186
                     /locus_tag="VFR25_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9941397.1"
                     /translation="MPSFRYRAAAAAGGTRVGEVEAVDLDQAIGRVRDLGLTPIQLDP
                     AGARAAGPRWALPKRRVSASELILFTRQLETMLDAGLPLLAGLETLESQAVDASLRDA
                     VRGVRAAVERGSTLTEAMRAYPHCFPTLYTNLVHAGEEGGLLPAMLDRLGGVLEHQEE
                     TRQRIQSAVFYPLIVLSELLVAFIVLVKLVLPKFATLFRNLNAELPLPTRVLIAVSDF
                     FDAYGWQALVLAVLLGVGAVLWLKTERGRRRFDALLLGIPLFGPIMQKVAMARFARVL
                     AALVESGVPIVRAVAISRGVLGNRSLEVEIDRMRDGLIAGEGLAAPLQGSRTVPPLLV
                     RMLAVGEETGSLGKMLVRTARFYDRDVDYTVKNLATALEPALLVVLGISVLFTALAVF
                     LPLWNLMNAFRH"
     gene            2307..2687
                     /locus_tag="VFR25_09900"
     CDS             2307..2687
                     /locus_tag="VFR25_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9941398.1"
                     /translation="MHRTKSESGFTLIELVIVIVILGILAAVAIPKYEDMREQARVAT
                     IKGQLGAIRSAVAIQYARNALNGHGGFPPLDGTIFAEGRVPKEPINQVSNVKTTPGVD
                     GSGGWVYLQNTGIVKVNLNAYSSY"
     gene            2779..4785
                     /locus_tag="VFR25_09905"
     CDS             2779..4785
                     /locus_tag="VFR25_09905"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET9941399.1"
                     /translation="MTQKLWIRPALVSAAVAAGALALYSATLAPGVGAGDSGELLLAA
                     QTLGVPHPPGYPLWTLLARVAAMLPWGDVAGRVNALSAIFAALGAGLFHTLARRAGLR
                     PFAALVATALYAASITIWSAAVETEVYALAAPCFLLLVLLAWRARSRRTASMRSDALA
                     FFAIGLAPVVHQTLAFPATFLGIWMFERDARLARVLRGLVWSSIGSTLFLVVALRSGA
                     PGSFTFAPETGVLGAVDGILRQGYGALRQHPMSTRLALSELGAMAGNVAASIGAAAAL
                     LALLGAWLARRERATLRLVAVAAATIPVALVLLVGFLPDAEHVAQIAPFLAPVVAAVC
                     LMAGAGAQAAVRRVPHAFRWPVLAAAATCALATVIAHQGLSDRHDFDLADRYARDLLA
                     PLPQGATLILDGDNETFLTAYATRLRGLRPDVTLIHRRGYIFGDVYGLAGMPRSRWTE
                     IAHRADLERRRGSKVPVYYTTPPEDLIDAGVVFRQEGLVSRALPPLPDWKVPPRRVEP
                     KPWLPPADWPKSASLLGGKPKRYDFVTRKLAVSYSDAAARALWSQGLVVESIPWFEDA
                     ARVGYDIAGAHLNLATALATAGVPERALDELLTARAVAPLDPEPTARVAVFLAAAGRH
                     EDAARWFERAYRIDPLPTFATDAARAWARAGNVERSRYWRERTG"
     gene            4782..5303
                     /locus_tag="VFR25_09910"
     CDS             4782..5303
                     /locus_tag="VFR25_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9941400.1"
                     /translation="MIRAHASKRAARGFTLVELVLIISILGLLVVIAVPDRSAVDEVS
                     LRSAARRLASDLRYAQGQSIARRIRHGVRFEPAEKRYEVVATGRGEPIEDPGARGHAL
                     VVSFKDPSVAHGVTLVSASFDGAPEVSFDYFGVPSNIAGREIRRPGLVVLACGGMTSS
                     VEIAPGTGKVTVR"
     gene            5300..5686
                     /locus_tag="VFR25_09915"
     CDS             5300..5686
                     /locus_tag="VFR25_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9941401.1"
                     /translation="MKQAGFTLIEVVLILVIVGVAILPLSLLFANASMRSGEARDATT
                     AAQLAQAKMEEIAADKSSPARGFDYLDTANYPPENPVVAFPRFRRSVLVSADSTFDGV
                     RFRAVNVTVSTSAIPPVTLTTWFAGR"
     gene            5686..6195
                     /locus_tag="VFR25_09920"
     CDS             5686..6195
                     /locus_tag="VFR25_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9941402.1"
                     /translation="MALRPLSQEGFTLIELLIVIVVVSIAGLVLSGVLREAARTYAFI
                     DVEKELVQESRYAKERMTREVMGAARGGIRAAHPRSITVVDADSALVTFAWDGVRGGD
                     LVCVRDGSRAPLASNVDSLAFAYFDGSGRLLRSADLASLRRVSIYLRLARDGHEVSGV
                     GSAFLRSAT"
     gene            6192..>7424
                     /locus_tag="VFR25_09925"
     CDS             6192..>7424
                     /locus_tag="VFR25_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941403.1"
                     /translation="MSARLAAPDAGFSIVLVVSTILVLIVLGLGVVTLVTEDSDLSGT
                     QVASDQAFYVAQAGLEYAAEKAASSPTWNGLPSPGKAVGLGSFWIQPPDTLDPGKRPL
                     PHGRKRIVAVGKVGAATRVLEIQVAPGTISTVAGGDSSACDPEGLAVAPGGDLVVADP
                     LAHLVRRVDLVTGVVSIVAGTGVAGSTGDGGRSENARLKSPRGVCFGPGGDLFIADMG
                     AHVVRKVSAASGVITTVAGSGDPGSSGDGGPATSARLSAPCGIAVTQGGDLLIAERGG
                     NRVRLVSAEDGVISTVAGSGAAGYGGDSGPAASARLRAPEDVALGPNGDLYVADTGNN
                     AIRRVARATGIITTVAGTGAPGFGGDGGAAALASLAGPRALETSPGGDLYIADTGNER
                     IRRIEIATASIATVAGTGD"
BASE COUNT          937 a         2726 c         2625 g         1136 t
ORIGIN      
        1 tcccccagga cggccgcttc cgcatcaacg tgggagggcg cgacctcgac gtccgcgtgt
       61 cgaccttccc gacgattcac ggggagaacg tcgtgctccg cctcctcgac cgctcggccg
      121 cgctgatcgg gatggccgag ctcgggctcg ggcctacgcc gctcgagctg ctcgcgcgca
      181 tgatcgaccg tccgaacggg atggtgctcg tgaccggccc cacggggagc ggcaagacga
      241 ccacgctcta cgcctgcctg catcggatcc agggggtgga gcggaacatc gtgaccctcg
      301 aggatcccgt cgagatccac ctcccgtccg tgcggcagac gcaggtcgat cccgacgtcg
      361 ggctcacgtt cgcgcgcggg ctgcgggcgc tcctccgcca ggacccggac gtgatcatgg
      421 tgggcgagat ccgggacgcc gagacggccg agatctcggt gcgctcggcg ctcacggggc
      481 atctcctcct cgcgacgctc cacacgaacg acgcggccgg cgcgattccg cggctcctcg
      541 acatggacac cgaaccgttc ctcctgtcgt ccgcgctcgc cggcgtggtg gcgcagcgcc
      601 tcgtgcggcg cgtctgcgac cgctgcaaga tgacggcgga cgtgccgcgc aacctgcgcg
      661 aggagctggg cctccccgcc gacgcgcgct tcctccgcgg caaggggtgc gtcgcgtgcg
      721 ggcacggcgg ctacaagggg cgcgtgggcg tgttcgaagt gctcgagatg accgacgcga
      781 tccgcgagcg gatccacacg cgcgcctcgg gcgaggagat cgcgcgcgcg gcgcgggacg
      841 cggggatggc cacgctcaag gacgatgcgc tccgcaaggc ggcgctcggc ctgaccacgc
      901 tcgacgaggc gctccgcgtc acggcggggg acagcgctcg ggcgcgggcc ggcgtggccc
      961 ggtagcgcgc catgccatcc ttccggtaca gggcggcggc cgccgcaggg ggaacccgcg
     1021 tcggcgaagt cgaggcggtg gatctcgacc aggcgatcgg ccgcgtccgc gatctcgggc
     1081 tcacgccgat ccagctcgat ccggccggcg cgcgcgccgc ggggccgcgg tgggcgctcc
     1141 cgaagcgacg cgtctccgcc tccgagctca tcctcttcac gcgccagctc gagaccatgc
     1201 tcgatgcagg gctgccgctc ctggccgggc tcgagacgct cgagtcccag gcggtggacg
     1261 cctcgctgcg cgacgcggtg cgcggcgtgc gcgcggcggt ggagcggggg agcacgctca
     1321 ccgaggcgat gcgcgcctac ccgcactgct tcccgacgct ctacacgaat ctcgtccacg
     1381 cgggggagga ggggggcctc ctgcccgcca tgctcgaccg tctcggcggg gtgctcgagc
     1441 accaggaaga gacgcgccag cggatccagt cggccgtctt ctatccgctg atcgtcctct
     1501 cggagctcct ggtggcgttc atcgtcctgg tcaagctcgt gcttcccaag ttcgcgaccc
     1561 tgttccggaa cctgaacgcg gagctcccgc ttccgacccg cgtcctgatc gcggtcagcg
     1621 acttcttcga cgcgtacggc tggcaggcgc tcgtcttggc ggtgctcctc ggcgtgggcg
     1681 cggtgctctg gctcaagacc gagcgcggcc gccgtcgctt cgacgcgctc ctcctcggca
     1741 tcccgctctt cgggccgatc atgcaaaagg tggcgatggc ccgcttcgcg cgcgtcctcg
     1801 cggcgctcgt cgagagcggc gtcccgatcg tgcgcgcggt cgcgatctcg cgcggggtcc
     1861 tgggcaaccg gagtctcgaa gtggagatcg accggatgcg ggacggcctc atcgccggag
     1921 aggggctggc agccccgctc caagggagcc gaaccgtgcc cccgctcctc gtccggatgc
     1981 tcgcggtggg ggaagagacc ggatccctgg gaaaaatgct ggttcggacc gcccggttct
     2041 acgaccggga cgtcgactac acggtcaaga acctggccac cgcgctggag cctgcgctcc
     2101 tggtggttct cggtatctcc gtgctgttca cggcgttagc cgtattcctc cccctctgga
     2161 acctgatgaa cgccttccgc cactagggct tgccgaacgc gttcaaggtg ggggggcgtc
     2221 gtgccgaaga ttcaagggtc aagggcccca ggcagccatt ccgccccagg ggcggggcgt
     2281 ctcgcaatcc aaggaggttg gccgttatgc accggacgaa gtcggagtcg ggcttcacgc
     2341 tgatcgagct ggtcatcgtg atcgtgatcc tgggcatcct cgcggccgtc gccattccga
     2401 agtacgagga catgcgcgag caggctcgcg tcgcaacgat caaggggcag ctgggcgcga
     2461 tccgctcggc ggtcgcgatc cagtacgcgc gcaatgcgct gaacggacac ggaggattcc
     2521 caccgctcga cggcacgatc ttcgccgaag ggcgcgtccc gaaggagccg atcaaccagg
     2581 tgagcaacgt gaagacgacg cccggcgtgg acggttccgg cggatgggtt tatctgcaga
     2641 acaccggcat cgtgaaggtc aacctgaacg cctactcgtc gtactgatcg cccccgcgac
     2701 gacgcgggag cacgaatcct ggcggggcgg gcccgactcc cgctccgcct cgaacgtttc
     2761 ggagaggccc tccctgccgt gacgcagaag ctctggattc gaccggctct cgtgagcgcc
     2821 gcggtcgccg cgggcgcgct cgcgctctat tccgccacgc tggctcccgg cgtgggggcg
     2881 ggggactcgg gcgagctcct gctcgcggcg cagaccctcg gcgtgccgca ccctcccggc
     2941 tacccgctct ggaccctcct cgcgcgcgtc gccgcgatgc tcccgtgggg cgacgtcgcc
     3001 ggacgggtga acgcgctctc ggcgatcttc gccgcgctgg gcgcgggcct cttccacacg
     3061 ctcgcgcgcc gcgcggggct ccgccccttc gcagcgctcg tcgcgaccgc gctctacgcc
     3121 gcgtcgatca cgatctggag cgccgcggtg gagaccgagg tctacgccct cgccgctcca
     3181 tgcttcctcc tgctcgtgct cctcgcctgg cgcgcccgct cccggcgcac ggcttcgatg
     3241 cggagcgacg cgctcgcctt cttcgcgatc ggcctggcgc cggtcgtcca ccagacgctc
     3301 gcattcccgg ccacgttcct cggcatctgg atgttcgagc gggacgcgcg gctcgcccgc
     3361 gtcctgcgcg gcctcgtctg gtcgtcgatc ggatccacgc tcttcctcgt cgtcgcgctc
     3421 cggagcggcg cgccgggaag cttcacgttc gcccccgaaa ccggcgtcct gggcgccgtg
     3481 gacgggatcc tgcgtcaggg ctatggcgcg ctccgccagc accccatgtc cacccgcctc
     3541 gcgctctcgg agctgggggc gatggcggga aacgtcgccg cgtcgatcgg cgcggcggct
     3601 gcgctcctgg ccctcctcgg cgcctggctc gcgcgccgcg agcgcgcgac gctgcgcctc
     3661 gtcgccgtgg ccgccgcgac tattccggtg gcgctcgtcc ttctcgtcgg attcctgccc
     3721 gacgccgagc acgtcgcgca gatcgcgccg ttcttagcgc cggtcgtggc cgccgtctgc
     3781 ctcatggccg gagccggcgc gcaggccgcc gtgcgccgcg tcccgcacgc gttccgctgg
     3841 ccggtcctgg ccgccgccgc gacgtgcgcg ctcgcgaccg tgatcgcgca ccaggggctc
     3901 tcggatcgtc atgacttcga cctcgccgac cgctacgcgc gcgatctcct cgctcccttg
     3961 ccgcagggcg cgacgctcat cctcgacggg gacaacgaga ccttcctgac cgcgtacgcg
     4021 acccggctgc gcgggctccg tcccgacgtc accctgatcc accgtcgcgg gtacatcttc
     4081 ggcgacgtct acggtctcgc gggcatgccg cggtcgcgct ggaccgagat cgcgcatcgc
     4141 gcggatctcg agcgccggcg cgggagcaag gtccccgtct actacacgac gccccccgag
     4201 gatctgatcg acgcgggcgt cgtgttccgc caggaagggc tcgtcagccg ggccctcccg
     4261 ccgctcccgg actggaaggt gccgccccgg cgcgtcgagc cgaagccgtg gctcccgccc
     4321 gccgactggc cgaagagcgc gtcgctcctc ggcggaaagc cgaagcgcta cgacttcgtg
     4381 acgcggaagc tcgcggtctc gtactcggac gccgcggcac gcgccctctg gagccagggg
     4441 ctcgtcgtgg aatcgatccc gtggttcgag gacgccgcgc gcgtgggcta cgacatcgcg
     4501 ggagcgcacc tcaatctggc caccgcgctc gcgacggccg gtgtgcccga gcgcgcgctc
     4561 gacgagctcc tcaccgcgcg cgccgtcgcg ccgctcgacc cggagccgac cgcgcgcgtc
     4621 gccgtcttcc tcgcggccgc cggccgccac gaagacgcgg cccgctggtt cgagcgcgcc
     4681 tatcgcatcg atccgcttcc caccttcgcc accgacgcgg cgcgagcctg ggcgcgcgcg
     4741 gggaacgtcg agcggtcgcg gtactggagg gagcgcaccg gatgatccgg gcccacgcat
     4801 cgaagcgcgc cgcgcgcgga ttcacgctgg tcgagctcgt gctcatcatc tcgatcctcg
     4861 gactcctcgt cgtcatcgcc gttcccgacc ggagcgcggt ggacgaggtg tcgctccggt
     4921 cggcggcgcg gaggctcgcg tccgatctcc gctacgcgca ggggcagtcg atcgcgcggc
     4981 gcatccgcca cggcgtccgg ttcgagccgg cggagaagcg ctacgaggtc gtggcgacgg
     5041 gacgcggcga gccgatcgag gaccccggag cgcgcggcca cgcgctcgtc gtctccttca
     5101 aggatccgtc cgtcgcgcac ggcgtcacgc tcgtgtcggc ctcgttcgat ggcgcgcccg
     5161 aggtgtcgtt cgactatttc ggcgtgccga gcaacatcgc cgggcgggag atccggcgcc
     5221 ccggcctcgt cgtgctcgcg tgcggcggga tgacgtcctc ggtcgagatc gcgccgggca
     5281 ccggcaaggt gaccgtccga tgaagcaggc cgggttcacc ctcatcgagg tcgtcctgat
     5341 cctcgtgatc gtgggggtcg cgatcctgcc gctcagcctt ctcttcgcga acgcgtcgat
     5401 gcgctccgga gaggcgcggg acgcgaccac ggcggcgcag ctcgcgcagg cgaagatgga
     5461 ggagatcgcc gcggacaaga gctcgcccgc gcgcggattc gactacctcg acacggccaa
     5521 ctatccgccg gagaacccgg tggtcgcgtt ccctcggttc cggcggtcgg ttctcgtgtc
     5581 ggccgactcg accttcgacg gcgtgcggtt ccgcgcggtc aacgtcacgg tgtccacgtc
     5641 cgcgattccg ccggtgacgc tgaccacctg gttcgcgggg cgctgatggc cctgcgtccg
     5701 ctgtcccagg agggcttcac gctgatcgag ctcctcatcg tgatcgtcgt cgtgtcgatc
     5761 gccgggctcg tcctctcggg cgtgctccgc gaagccgcgc gcacgtacgc cttcatcgac
     5821 gtggagaagg agctggtcca ggaatcgcgc tacgccaagg agcgcatgac ccgcgaggtc
     5881 atgggggccg cccgcggcgg gatccgcgcg gcccatcccc gatcgatcac cgtggtcgac
     5941 gcggactccg ccctcgtgac gttcgcgtgg gacggcgtcc gcggcggcga tctggtctgc
     6001 gtgcgggacg gatcgcgcgc cccgctcgcg tcgaacgtcg actcgctcgc cttcgcctac
     6061 ttcgacggct ccggccgcct gcttcgatcg gcggacttgg cgagccttcg ccgcgtctcg
     6121 atctatctcc gcctcgcgcg ggacggtcac gaggtctcgg gtgtggggag cgcttttctg
     6181 agatccgcga catgagcgcc cgcttggccg cgcccgacgc cgggttctcg atcgtgctcg
     6241 tcgtgtcgac gatcctcgtc ctcatcgtcc tcggcctggg cgtcgtcacg ctcgtcaccg
     6301 aggactccga tctctccggc acgcaggtcg cctccgatca ggccttctac gtggcgcagg
     6361 ccggcctcga gtacgcggcc gagaaggcgg cctcgtcgcc gacgtggaac gggctcccgt
     6421 cccccggcaa ggcggtgggc ctaggctcgt tctggatcca gccgcccgac accctcgacc
     6481 ccggcaagcg gccgcttccg catggacgca agcgcatcgt cgcggtgggc aaggtgggcg
     6541 cggcgacgcg cgtgctcgag atccaggtcg cgcccggaac gatcagcacg gtggccggcg
     6601 gggactcgag cgcgtgcgat cccgaaggac tcgcggtggc ccccgggggc gatctcgtcg
     6661 tggccgaccc cctggcccat ctcgtgcggc gcgtcgacct ggtgaccggc gtcgtctcga
     6721 tcgtggccgg aaccggagtg gcgggctcga ccggcgacgg cggccgctcc gagaacgcgc
     6781 ggctcaagtc gcctcgcggg gtctgcttcg gtccaggggg cgatctcttc atcgccgaca
     6841 tgggggcgca cgtggtgcgc aaggtatccg ccgcgtcggg cgtgatcacg accgtcgcgg
     6901 gaagcggcga cccgggatcg tcgggggacg gcggtcccgc gacctccgca cggctctccg
     6961 ctccgtgcgg catcgcggtc acgcagggag gcgatctcct gatcgcggag cggggaggga
     7021 atcgcgtgcg gctcgtctcc gcggaggacg gggtcatctc caccgtcgcg gggagcggcg
     7081 ccgccggcta cggcggcgac tccggccccg ccgcttcggc acggctgcgc gcgcccgagg
     7141 acgtcgcgct cggccccaac ggagacctct acgtggccga cacggggaac aacgccattc
     7201 gccgcgtcgc gcgcgcgacc gggatcatca cgaccgtggc gggcaccgga gcgccgggct
     7261 tcggcgggga cggcggcgcc gcggcgctgg catcgctcgc gggaccgcgc gcgctcgaaa
     7321 ccagccccgg cggggacctc tacatcgcgg acaccgggaa cgagcggatt cgccggatcg
     7381 agatcgcgac ggcttccatc gcgaccgtcg cgggaacagg cgat
//
LOCUS       DASTKC010000236         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_205629, whole genome shotgun sequence.
ACCESSION   DASTKC010000236 DASTKC010000000
VERSION     DASTKC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205629"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VFR25_09930"
     CDS             <1..1449
                     /locus_tag="VFR25_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9941404.1"
                     /translation="FPMMQFGRAVFAPEYGSIIYWGGGGHGHSRVGNDVWLYDTARNE
                     WRQVTPGDPIESYPNPGLPTPNLGDYCNNDSSFAYFWSACQPTANLPIGSTASGAPWS
                     SHNYSQRAWDSYNRRLVIYGPNYLIGLMPDSNAWFAARASYTFDPYFRSWRFLANNPH
                     LYHQGGAMAFDPVNLRMVAVNHDWWHHAGYNSSADRFNFRWWLDVTTDTWTEKPPVLA
                     PNMFDSDLVYDPDTRKMYRFGGDFPTDSDLWCYDQEEDTWTLRSPIPDPVHGVPPAAA
                     PSAAMGRNGTMLLWGFGGDGECLPTWAYNTRLNVWKKMDAANDPITDAPANDRTHAWA
                     SLVYDPTNNVFFLLKIDPQETPNDVGGWPNSLVGQIWVYKHDGGPSATTGVSEAPSPR
                     PHLAQNAPNPFASRTGIAFTLDEPAQVTLTIFDVRGRVVARLADRAMTPGPAQLVWDG
                     RDSQGHAAAAGVYWYQLRAGSFRAARKMVLLR"
     gene            complement(1593..1979)
                     /locus_tag="VFR25_09935"
     CDS             complement(1593..1979)
                     /locus_tag="VFR25_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941405.1"
                     /translation="MASPGEKKFWLLRRLARAFNVAAWIVLGLVVLVTPVAVARFLLQ
                     RNQEEVVFWLSYAASGLLIFINLIWVAQSIQVILAIEENTRHATFVLEKLTTLAQQIR
                     DRLPDGEAGTTGSDAGVGGLGPPGVR"
     gene            complement(1979..2344)
                     /locus_tag="VFR25_09940"
     CDS             complement(1979..2344)
                     /locus_tag="VFR25_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941406.1"
                     /translation="MPQGAVPDDEIERGRILASVAYLPGLCFIGLLGAPDNRYVGFHA
                     RQGFLLFLVEVVIAIALAIYDGSIGMIPVVGFLVGALLKFILWMSILALTIYGVVKGA
                     SGEMARIPVLGEAVEKVPF"
     gene            complement(2410..>4125)
                     /locus_tag="VFR25_09945"
     CDS             complement(2410..>4125)
                     /locus_tag="VFR25_09945"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HET9941407.1"
                     /translation="LAWVDPTFREAEPLRALMEKGRGFTEEEKGMLLDWGVTCARDAA
                     LDYRKAAAAGQVELTTSAYNHPILPLLIDSMAPREASPGAVLPDPPFRAPEDAAAQIA
                     RARESHGRRFGAPPKGTWPPEGAVNDAALAALARGGFAWAASDETVLQGALATRDGSV
                     RDWPAALYRPYRLETAGGPIAMVFRDRALSDRIGFTYASWNPEDAARDFVERVRAAGD
                     SALGDGPGLVPVILDGENCWESYLDDGRPFLEALYGLLERDGQIEAVTVSEAIERVPP
                     AETLERVPVGGWVRADLGIWIGQEDKNRAWVELTRARAAVEEARRSRAADADLAMEEI
                     YAAEASDWFWWYGDDHPSAHRAMFDRLFRSRLARAYERIGAAVPDSLRSSLRRDADQA
                     EADLAGVSGHAPSAFPFVKPTIDGRSSDFYEWMGAIEHGASGESGAMHRASAIVRSVR
                     FGGDGLNLYVWIDADPRAAAGSVIAIDTALGRVARVPLQAGRGAPSWNGGLAGGDAGE
                     YAAAEVVELRLPLAALGARAGEPTAFRVSLERDGAREEIVPAAGWLTLPAAGAHPELD
                     LWSAT"
BASE COUNT          728 a         1488 c         1287 g          622 t
ORIGIN      
        1 tttcccatga tgcagttcgg gcgcgccgta ttcgcacctg agtatggatc catcatctac
       61 tgggggggtg ggggacacgg ccattcgcgc gtggggaacg acgtttggct gtacgacacc
      121 gctcggaacg agtggcgcca ggtcactccg ggtgatccga tcgagtctta tcccaatccg
      181 gggctgccca ctccgaacct cggcgactac tgcaacaacg acagctcgtt cgcgtacttc
      241 tggtccgcat gtcagcccac cgcaaatctc cccatcggat cgacggcttc cggtgcgccg
      301 tggagctccc acaactactc gcagcgcgcg tgggactcct acaaccgccg cttggtcatc
      361 tacggtccca actaccttat cggcctcatg cccgactcga acgcgtggtt cgcagcccgg
      421 gcctcctaca ccttcgatcc gtacttccga tcgtggaggt tcctcgcgaa caacccacat
      481 ctctatcacc agggcggcgc catggcgttc gacccggtga acctccggat ggtggcggtg
      541 aaccacgact ggtggcacca cgccggctac aactcgagcg cggaccgctt caactttcgc
      601 tggtggctcg acgtgacgac ggacacgtgg accgagaaac cgcccgtcct cgcaccgaac
      661 atgttcgaca gcgacctcgt gtacgacccc gacacccgaa agatgtatcg cttcggtggg
      721 gattttccga cagacagcga tctctggtgc tacgaccagg aagaggacac atggacgctc
      781 cggtcaccga tccctgaccc ggtccatggc gtccctccgg cggctgcgcc gtcggcggcg
      841 atgggacgaa acggaacgat gctcttgtgg ggtttcggag gagacggcga gtgccttcca
      901 acatgggcct acaacacgag gctcaacgtc tggaagaaga tggacgccgc caacgacccg
      961 atcacggacg ctcctgcgaa tgaccggacc catgcctggg cgtccttggt gtacgacccc
     1021 acgaacaacg tctttttcct tctaaagatt gatccgcagg agaccccgaa cgacgtcggc
     1081 ggatggccca acagcctcgt agggcaaatc tgggtctaca agcacgatgg tgggccctcg
     1141 gcaacgaccg gcgtctccga ggccccatct ccgcgcccac acctcgctca aaacgccccc
     1201 aacccctttg catccagaac cgggatcgcg ttcaccctgg acgagcccgc gcaggtgacc
     1261 ctgacgatct tcgacgtacg ggggcgggtt gtcgcaaggt tggccgaccg agccatgacc
     1321 ccggggccgg ctcagctagt ctgggacggc cgcgacagcc aaggccacgc cgcggccgca
     1381 ggggtctact ggtaccaact gagagcgggc agtttccgag ccgcccgcaa gatggtcctt
     1441 ctgaggtagg gggccccgac ccagcccggg ctggcgcccg gactatttga tgtcaacttc
     1501 cacccaagcc agcgctggag aggctgcgac gcccgtggtg gaagttgaca taatagttct
     1561 tatccgacaa accttggtac gcccagaggc gcctaccgaa cccccggcgg tcccaacccg
     1621 ccaacccccg catccgaccc cgtcgtcccc gcctcgccgt ccggcagccg gtcccggatc
     1681 tgctgcgcca gggtcgtcag cttctccagc acgaacgtgg cgtgccgggt gttctcctcg
     1741 atcgccagga tgacctggat cgactgggcc acccagatca gattgatgaa gatcaggagc
     1801 ccggaagccg cgtagctcag ccagaagacg acttcctcct ggttccgctg cagcaggaac
     1861 cgggcaaccg cgaccggcgt caccaggacc accagcccga gaacgatcca ggcggccacg
     1921 ttgaaggccc tcgccaagcg ccgcaggagc cagaacttct tctcgccggg gctggccatc
     1981 agaacggaac cttctcgacc gcttcgccca gaaccggaat tcgggccatc tcgccggatg
     2041 ctcctttcac aacgccgtaa atggttaacg cgaggatgct catccaaagg atgaacttga
     2101 ggagcgcccc caccagaaat cctacgaccg ggatcattcc gatggaaccg tcgtagatcg
     2161 caagcgcgat cgcgatcacg acctcgacca ggaagaggag gaagccctgg cgcgcgtgga
     2221 acccgacata gcgattgtcg ggcgcgccga gcaggccgat gaagcaaagc cctggcagat
     2281 aggcgaccga cgcgaggatc cgtccccgct cgatctcgtc atccgggaca gcgccttgcg
     2341 gcatgtcgac gttcacggcg cacgaatagc acgcgacacc ggaggcgtca agcaaaccgg
     2401 gacgcgcggt caggtcgcgg accagaggtc cagctccgga tgggcgccgg ccgcgggcag
     2461 ggtcagccat ccggccgcgg gcacgatctc ctcgcgcgcg ccgtcccgct cgagcgacac
     2521 ccggaacgcc gtgggctccc cggcccgcgc gccgagcgcg gccaggggaa gccgaagctc
     2581 cacgacctcg gccgcggcgt actcccccgc gtcgcctccg gccaaaccgc cgttccatga
     2641 aggcgccccg cgccctgctt ggaggggaac gcgcgcgacg cgtcccaggg cggtgtcgat
     2701 cgcgatcacc gatcccgccg ccgcgcgcgg atcggcgtcg atccagacgt agaggttgag
     2761 tccatcaccc ccgaagcgca cggagcgcac gatcgcggac gcccggtgca tggctccgct
     2821 ctcgccggac gcgccgtgct cgatcgcgcc catccattcg tagaagtcgg agctccgtcc
     2881 gtcgatcgtc ggcttcacga acgggaacgc ggacggagcg tgtcccgaga cgccagccag
     2941 atccgcctcc gcctggtccg cgtcgcgacg aagactcgac ctcagtgaat cgggaacggc
     3001 cgcgccgatg cgctcgtacg cgcgcgcgag gcgcgatcgg aagagccggt cgaacatcgc
     3061 ccgatgcgcg ctcggatgat cgtcaccgta ccaccagaac cagtcgctag cctcggcggc
     3121 atagatctcc tccatggcga ggtccgcatc ggccgcgcgc gatcgccgcg cctcctccac
     3181 cgcggcgcgc gctcgcgtga gctccaccca cgcgcggttc ttgtcctcct gcccgatcca
     3241 gattccgaga tccgcgcgga cccatcctcc caccggcacg cgctccagcg tctcggccgg
     3301 cggcacgcgc tcgatcgctt ccgacaccgt caccgcttcg atctggccgt cacgctcgag
     3361 caggccgtag agcgcttcca ggaacggccg tccgtcgtcg agataggact cccagcagtt
     3421 ctccccgtcc aggatcacgg ggacgagccc aggaccgtcg cccaaggcgg agtcccccgc
     3481 cgcgcgcact cgctcgacga agtctcgcgc ggcgtcctct ggattccagc tcgcgtacgt
     3541 gaatccgatc cggtccgaga gcgcgcggtc gcggaacacc atggcgatcg ggcctccggc
     3601 ggtctccaag cgatacggac ggtagagcgc ggcgggccag tcgcgcacgg agccgtcccg
     3661 ggtggcgagg gctccctgga gcacggtctc gtcggatgcc gcccacgcga acccgccgcg
     3721 tgcgagcgcg gcgagcgcgg catcgttcac cgcaccctcc ggcggccagg tgcccttggg
     3781 gggcgcgccg aagcgcctcc cgtgcgactc gcgcgctcga gcaatctgcg cggccgcgtc
     3841 ctccggcgcg cgaaacggcg ggtccggcag cacggcgccg ggcgacgcct cccgaggcgc
     3901 catcgagtcg atgaggagcg ggaggatcgg atggttgtag gccgacgttg tgagctccac
     3961 ctgcccggcc gcggctgcct tccgataatc gagagccgca tcgcgcgcgc aggtcacgcc
     4021 ccagtcgagg agcatcccct tctcctcctc ggtgaatccc ctccccttct ccatcagcgc
     4081 gcggagcggc tcggcctcgc gaaacgtggg atcgacccag gcgag
//
LOCUS       DASTKC010000237         6023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_915446, whole genome shotgun sequence.
ACCESSION   DASTKC010000237 DASTKC010000000
VERSION     DASTKC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915446"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VFR25_09950"
     CDS             complement(<1..153)
                     /locus_tag="VFR25_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9941408.1"
                     /translation="MSRPFEGRVAVVTGGAKGIGRAIAVHLASGGAKLVLAGRSEGPL
                     EELKAEI"
     gene            complement(150..1097)
                     /gene="fabD"
                     /locus_tag="VFR25_09955"
     CDS             complement(150..1097)
                     /gene="fabD"
                     /locus_tag="VFR25_09955"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9941409.1"
                     /translation="MVGPALLFPGQGSQSVGMGKALAERYPKARAVFEDADRILGFAL
                     SKLCFEGPIEELTRTENTQPALLVTSLAAYRVLEDRGVRPAAAAGHSAGEYAAHVAAG
                     SLPFDEGLRLIRLRGEAMAQAGSDRPGTMAAVLGLSAEEIDEVLRTVNAPHDLAAANY
                     NAPGQVVLSGTPEAVARGTEEAKKAGAKKIVPLQVSAAFHSPLMEGAARRLGEGIDKA
                     PVGRAAFPVYANVTAGPVTEPDAIRRTLREQLLQPVRWEQTMRAMVEQGIRRYIEVGN
                     GRVLRGLLRGVDKEAQVFGTDDPDALEAAVQGLAEAAAR"
     gene            complement(1091..2023)
                     /locus_tag="VFR25_09960"
     CDS             complement(1091..2023)
                     /locus_tag="VFR25_09960"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9941410.1"
                     /translation="MLTNQDLEQIVDTSDEWITSRTGIKERRISDPATPSSVLATKAA
                     ERALEIAGLKGADLDQIVVGTVTGDRTFPSTACIVQDRLGATRAHAFDISAACAGFLY
                     GLSVGRSAIESGAADTVLVIGVETLSKIVNWTDRNTCVLFGDAAGAVVLRSTGKPGGI
                     LATRLHSDGSLVHLLEMPAGGSLMPPSHETVAQRLHTIHMSGNDVFKHAVRAMESVAL
                     EALEAAGRKPEDLSLLVPHQANYRIIDATARRLGLPMEKVFVNLDRYGNTSAASIPLA
                     LDEANRGGRIRKGDLVEMVTFGGGFTWAAAVVEW"
     gene            complement(2080..3111)
                     /gene="plsX"
                     /locus_tag="VFR25_09965"
     CDS             complement(2080..3111)
                     /gene="plsX"
                     /locus_tag="VFR25_09965"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET9941411.1"
                     /translation="MGRQGTGPVIGVDAMGGDFAPRVVIQGALEALQESGAMFDVALV
                     GDESAIVEEADRLGVRSRLPRIIHASEQVEMAESAAASVRKKRDSSISVSARLQKDHE
                     TDALVSAGNTGAVVAAALFDLGRIESIQRPAIATVLPTPQGNVVVLDVGATSDPKPVH
                     LYQFALMGSIYARLVLHVERPRVGLLNIGEEAEKGSELYYEAHQLLKRSPVHFVGNVE
                     GRDIILGTADVVVCDGFVGNVLLKFAESVIPSISGMIKDEIQRHPLSMMAGLLLKPAF
                     RRLRRRLDYSEVGGAPLLGVDGTCIIAHGRSNVRAIKNAIRVAARCAEAKVADSIRAE
                     LQRIAPEAA"
     gene            complement(3132..3323)
                     /gene="rpmF"
                     /locus_tag="VFR25_09970"
     CDS             complement(3132..3323)
                     /gene="rpmF"
                     /locus_tag="VFR25_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9941412.1"
                     /translation="MAVPKRRHSSTRGKKRRTNWKLSVSARSLCSHCSQPKLPHRVCG
                     HCGYYAGEEIVTREASKTT"
     gene            complement(3386..3868)
                     /locus_tag="VFR25_09975"
     CDS             complement(3386..3868)
                     /locus_tag="VFR25_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET9941413.1"
                     /translation="MQIDLKQLPQGVSTLDYELPPASVGITPQDVTVEGPLKVRLSLD
                     RRGDEIWVRGKLQLVAVQECSRCLTDFKEPLDLAFEVFCAKVHSPNVVSASVVDEDDE
                     GVHVHDGRILSVDHEIREAVILGLSMKPLCKETCAGLCPTCGDDRNLGPCRCARAAAG
                     "
     gene            complement(3906..4301)
                     /gene="ndk"
                     /locus_tag="VFR25_09980"
     CDS             complement(3906..4301)
                     /gene="ndk"
                     /locus_tag="VFR25_09980"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002724748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET9941414.1"
                     /translation="MEETLFMIKPDAVAGKKVGLILAEIEKAGFEILAMRLVQLTPEN
                     ARRFYAVHEGKPFLDDLVRFMSSGPAVPCRLRRDNAILFLRERIGATDPKEAKPGTIR
                     ALYAESKQNNAVHASDSPESARTEIPFFF"
     gene            complement(4607..5458)
                     /gene="sucD"
                     /locus_tag="VFR25_09985"
                     /pseudo
     CDS             complement(4607..5458)
                     /gene="sucD"
                     /locus_tag="VFR25_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            complement(5455..>6023)
                     /gene="sucC"
                     /locus_tag="VFR25_09990"
     CDS             complement(5455..>6023)
                     /gene="sucC"
                     /locus_tag="VFR25_09990"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HET9941415.1"
                     /translation="PLIVTPDGEVRALDSKFTVDDSALFRHPDIAEMRDEDAADPLEA
                     LARAKHVTYVKLDGEVGVLGNGAGLTMSTVDVVTFAGGRPANFCDLGGGGDAQGVVDA
                     LEVITADPQVKAIFFNIFGGITRCDDVARGLLTALDRICTKTPIVIRLTGTNEKEARD
                     LLGARGMTALSDMDEAVRAVIARAAEAA"
BASE COUNT          895 a         2141 c         1996 g          991 t
ORIGIN      
        1 gatctcggcc ttgagctcct cgagcggtcc ctcggagcgc cccgcgagga cgagcttcgc
       61 gccccccgac gcgaggtgga ccgcgatggc ccgcccgatc cccttcgcgc cgcccgtgac
      121 cacggccacg cgtccctcga acggccggct caccgcgccg ccgcctccgc gagcccttgg
      181 accgcagctt cgagcgcgtc cgggtcgtcc gtgccgaaca cctgcgcttc cttgtcgacg
      241 ccgcgaagca gtccgcgcag cacgcgtccg tttccaacct cgatgtagcg ccggatgccc
      301 tgctccacca tcgcgcgcat cgtctgctcc cagcgcaccg gctggaggag ctgctccctc
      361 agcgtgcgcc ggatcgcgtc cggttccgtc accggccccg cggtcacgtt ggcgtagacc
      421 gggaacgccg cgcgcccaac cggcgccttg tcgattcctt cgccgagacg ccgggcggct
      481 ccttccatga gcggcgagtg gaacgccgca cttacttgta aagggacgat cttcttcgcc
      541 cccgccttct tcgcttcctc ggtgccccgc gcgaccgcct cgggcgtgcc cgagaggacc
      601 acctgccccg gggcgttgta gttcgccgcg gcgagatcgt gcggcgcgtt gaccgtgcgg
      661 agcacctcgt cgatctcctc agcggagagc ccgaggacgg ccgccatcgt tcccggacga
      721 tccgagcccg cttgggccat cgcttcgccg cggagccgga tgagccggag cccctcgtcg
      781 aacgggagcg atcccgccgc cacgtgcgcg gcgtactcgc ccgcgctgtg ccccgcggcc
      841 gccgccggcc ggacgccccg gtcctcgagc acgcgatagg cggccaggct cgtcacgagc
      901 agcgcgggct gcgtgttctc ggtgcgcgtc agctcttcga tcggcccctc gaagcacagc
      961 ttcgagagcg caaaaccgag gatccggtcg gcatcctcga agacggcgcg tgccttggga
     1021 tagcgctccg cgagcgcctt gcccataccg accgactgcg atccctggcc gggaaagagg
     1081 agcgctggac ctaccactcg accaccgccg cggcccatgt gaaaccgccg ccgaaggtca
     1141 ccatctccac gagatccccc ttccggatgc gtcccccgcg attggcttca tcgagcgcga
     1201 gcgggatcga cgccgccgag gtattgccat agcggtcgag gttcacgaag accttctcca
     1261 tcggaagacc caggcggcgc gcggtggcgt cgatgatccg gtagttcgcc tggtgcggca
     1321 cgaggagcga gaggtcctcg ggcttccgtc ccgcggcctc gagcgcctcg agcgcgaccg
     1381 actccatggc gcggaccgcg tgcttgaaca cgtcgttccc gctcatgtgg atcgtgtgga
     1441 gccgctgcgc gaccgtctcg tgcgagggcg gcatgagcga gcccccggcc ggcatctcga
     1501 ggagatgcac gagcgagccg tcgctgtgga gccgcgtcgc gaggattccc cccggcttgc
     1561 cggtcgagcg gagtaccacg gctcccgccg cgtcgccgaa gagcacgcaa gtgttccggt
     1621 cggtccagtt gacgatcttc gagagcgtct ccactccgat gacgagcacc gtgtcggcgg
     1681 cgcccgactc gatcgcgctc cgtcccacgg agagcccgta gaggaagcca gcgcatgcgg
     1741 cggagatgtc gaaggcgtgc gcgcgcgtcg cgccgagccg gtcctgcacg atgcacgccg
     1801 tcgacggaaa ggtgcggtcc cccgtcacgg tgcccaccac gatctgatcg agatccgctc
     1861 ccttgaggcc cgcgatctcg agcgcgcgct ccgccgcctt ggtcgcgagg accgaggagg
     1921 gcgtcgccgg atcggagatc cgccgctcct tgatcccggt gcggctcgtg atccactcgt
     1981 cggatgtgtc gacgatctgc tccaggtcct gattggtcag cactttgtcg ggaacgtggg
     2041 agccggtccc gatgatgtgg acgtcacggc gaggggcgat caggctgcct ccggagcgat
     2101 ccgttgcagc tcggcgcgga tggagtcggc caccttggcc tcggcgcatc gcgccgccac
     2161 ccggatcgcg ttcttgatgg cgcggacgtt cgagcggccg tgggcgatga tgcaggttcc
     2221 gtcgacgccg agcagcggcg cgccccccac ctcggagtag tcgaggcggc ggcgcaggcg
     2281 gcggaacgcg ggcttcaaga ggaggcccgc catcatcgag agcggatggc gctggatctc
     2341 gtccttgatc atgcccgaga tcgacgggat cacgctctcg gcgaacttga ggagcacgtt
     2401 gcccacgaag ccgtcacaga caacgacgtc cgcggtgccg aggatgatgt cccgtccttc
     2461 cacgttgccg acgaagtgga ccgggctccg cttcaagagc tgatgcgcct cgtagtagag
     2521 ctcgcttccc ttctccgcct cctcgccgat gttgaggagc cccacgcgcg ggcgttcgac
     2581 gtgaagcacg agccgcgcgt agatgcttcc catcaacgcg aactggtaga ggtggaccgg
     2641 cttgggatcg gaggtcgcgc ccacgtcgag cacgacgaca ttcccctgcg gcgtcgggag
     2701 cacggtggcg atggcgggac gctggatcga ttcgatccgg cccaggtcga agagcgcggc
     2761 ggcgacgacc gctccggtgt tgcccgcgga gacgagcgcg tccgtctcgt ggtctttctg
     2821 gagacgggcg gagacgctga tcgaggagtc gcgcttcttc cgcaccgacg cggccgcgga
     2881 ctcggccatc tcgacctgct cggaggcgtg gatgatgcgc ggaaggcggg agcggacgcc
     2941 gaggcgatcc gcctcttcga cgatcgccga ctcgtccccg acgagagcga cgtcgaacat
     3001 cgctcccgac tcctgcaggg cctcgagagc cccctggatg acgacgcgag gggcgaagtc
     3061 gccccccatg gcgtcgacac cgatgaccgg tcctgttcct tggcggccca tgggtcagtc
     3121 ctctggacgc ctcaggtggt cttcgacgcc tcccgggtga cgatctcctc cccggcgtag
     3181 tagccgcagt gaccgcagac gcggtggggg agcttgggct ggctgcagtg ggaacagagc
     3241 gatcgcgccg acacgctcag cttccagttg gtgcggcgct tcttgccacg cgtgctcgag
     3301 tgccttcgct tgggaacggc cattcgtcgt gaaccctcgt gtcgagatgg aggcgggccc
     3361 ggcccggcga tccacggtca gcggatcacc cggcggccgc acgtgcgcag cggcaaggtc
     3421 ccaggtttcg atcgtcgccg catgtggggc aaagccccgc gcacgtttcc ttgcaaagcg
     3481 gcttcatgga gaggccgagg atcacggcct cccggatctc atgatcgacg gagagaatcc
     3541 ggccatcgtg gacatgcacc ccctcatcgt cctcatccac cacggacgcg ctcaccacgt
     3601 tggggctgtg caccttagca cagaagacct cgaacgccaa gtcgagcggc tccttgaagt
     3661 ccgtcaggca gcggctacat tcctgaaccg caacgagttg gagcttccct cgaacccaga
     3721 tctcgtcccc ccgccggtcg agcgagaggc gaaccttgag cggcccctcg acggtgacgt
     3781 cctggggcgt gattcccacc gatgcgggag gcagctcgta gtcgagcgtg ctgacaccct
     3841 gcggcagctg tttcagatcg atctgcatgg gccttgaacc gatgcgcggc gcccccggcg
     3901 ccccgctaga agaagaacgg aatctcggtc cgcgcggact cgggggagtc cgaggcgtgg
     3961 accgcgttgt tctgtttgga ttccgcgtag agcgcgcgga tcgtgcccgg cttggcctcc
     4021 ttgggatccg tcgcgccgat ccgctcacgc aggaacagga tcgcattgtc gcggcggagc
     4081 cggcagggaa ccgcgggacc ggaggacatg aaccgaacga ggtcgtcgag gaagggcttc
     4141 ccctcgtgca ccgcgtagaa acgccgcgca ttctcgggcg tgagctggac gagcctcatc
     4201 gcgaggatct cgaaacccgc cttctcgatc tccgcgagga tcagtccaac cttcttgccc
     4261 gccaccgcat ccggcttgat catgaacagt gtctcttcca ccgccattac acttcctcct
     4321 gggtcgctgt catgaaatgg gctcccccgc tcggggaagc ccattaagcg cgcgatcgcg
     4381 aagcgatcgc gcggaacgtc gatcaccgag cgcgcttcgc gcgcgctttg gtggccggct
     4441 ttgccttcgc cttggaccgg accttcgact tcgcggtctt ggccttggtc gttttcaccg
     4501 cgctcgcctt gcgcgatttc acggcggtct tcttcttcga ggccgccgcc ttcgctcgcg
     4561 acacgagctt gagccgcgag gggcgcttcg cccggagcgc ggccgcgatc aagcccggga
     4621 tctcggacgg caccttggcc accgggacgc ccgcgcgctc gaagcgcgcc atcttctcct
     4681 gcgccgtgcc cgcgcctccg gagacgatcg ctccggcgtg ccccatccgc ttgccgggcg
     4741 gggcggtctg tcccgcgatg aacgcgacca ccggcttcga gacatgcttc tcgatgagcg
     4801 ccgccgcgtc ctcctcgtcg gtgccgccga tctcgccgat caggacgatc gcgtcggtcg
     4861 aatcgtccgc ctcgaagagc gcgagcgtat ccacgaaccg cgtgcccacg atggggtcgc
     4921 cgccgatgcc gatgcacgtc gactgtccca tgcccgcgcg cgtgagctgc cagaccactt
     4981 cgtacgtgag cgtgccgctc cggctgacga ggccgatccg ccccggcgta tggatgaatc
     5041 cgggcatgat gccgaccttg cactgccccg gcgagatgag ccccgggcaa ttcggaccga
     5101 tcagccgcgt cgagcgcccc tcgagatagg aggcgacctc gatcatgtcg cgcgcgggga
     5161 gcccttccgt gatgcagacg acgagcggaa cctcggcgtc gaccgcttcg tagatggcgt
     5221 cctgcgcgag agccgccggc acgtagatca ccgacacgtc ggcgcgcgtg cgcgccaccg
     5281 cttccttgac cgagtcgaag accgggatcc cgccgacgtc ggtgcctccc ttgcccggag
     5341 tcacgcccgc gacgaccttc gtcccgtatt ccttcatctg cttcgcgtgg aaggaaccgt
     5401 ccctcccggt gatcccctgc accagaacct tcgacccccg cccgaccagg atgctcatgc
     5461 cgcctccgcc gcgcgggcga tcaccgcgcg caccgcttcg tccatgtcgc tcaaggcggt
     5521 catgccgcgc gcgcccagga gatcgcgcgc ctccttctcg ttcgtgccgg tgagccggat
     5581 cacgatcggc gtcttcgtgc agatccggtc gagggcggtc aggagcccgc gcgcgacgtc
     5641 gtcgcaacgc gtgatgccgc cgaagatgtt gaagaagatc gctttcacct gagggtcggc
     5701 ggtgatcacc tcgagcgcgt cgacgacccc ctgcgcgtcg ccgccgccgc cgaggtcgca
     5761 gaagttcgca ggacggccgc cggcgaacgt cacgacgtcg acggtcgaca tcgtgagacc
     5821 cgctccgttc ccgaggacgc cgacctcgcc gtcgagcttg acgtacgtga cgtgctttgc
     5881 ccgggcaagg gcctcgagtg ggtcggcggc atcctcgtcg cgcatctcgg cgatgtccgg
     5941 atgccggaag agcgcgctgt cgtccaccgt gaacttcgag tcgagcgcgc gcacctcgcc
     6001 gtccggcgtg acgatcagcg gat
//
LOCUS       DASTKC010000238         6282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_915772, whole genome shotgun sequence.
ACCESSION   DASTKC010000238 DASTKC010000000
VERSION     DASTKC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915772"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="rplB"
                     /locus_tag="VFR25_09995"
     CDS             <1..526
                     /gene="rplB"
                     /locus_tag="VFR25_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9941416.1"
                     /translation="IIAPQLLKVGDKILSGPDAEVKPGNSMPIGAVPLGTFLYNVELV
                     PGKGGQLSRSAGTYCQLMAKEGDYAQLRLPSGEMRKVPVVCSCSIGQVGNEEHQNIII
                     GKAGKSRWLGRRPSVRGVAMNPVDHPMGGGEGKSSGGRHPCSPWGLLAKGKKTRKRKL
                     SDALIVRRRKSKGA"
     gene            557..850
                     /gene="rpsS"
                     /locus_tag="VFR25_10000"
     CDS             557..850
                     /gene="rpsS"
                     /locus_tag="VFR25_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET9941417.1"
                     /translation="MSRSVKKGPFVDAKLLKRLEAMNRVNERRVLKTWARRSTISPEF
                     VGHTLAVHNGNKFIPVYITENMVGHKLGEFAATRIFRGHGSHVEKAAGPPAAK"
     gene            874..1431
                     /gene="rplV"
                     /locus_tag="VFR25_10005"
     CDS             874..1431
                     /gene="rplV"
                     /locus_tag="VFR25_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET9941418.1"
                     /translation="MEAKAIARYVRVTPRKTDQVLQLIRGKRVDQATEILDFASKHVA
                     KVIGKVLKSAVANAVAIEGKINIEELRVKQATAGAGPTMKRFLPRAQGRATPLLKRTC
                     HITIVVEGHVDEAAHAPHRARRTGKVAAPVAPAKAAVQAPAVETKAAKAPARKKAAKP
                     AAKKSSPAKASAKPASKAKPKKGAK"
     gene            1431..2111
                     /gene="rpsC"
                     /locus_tag="VFR25_10010"
     CDS             1431..2111
                     /gene="rpsC"
                     /locus_tag="VFR25_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET9941419.1"
                     /translation="MGQKTNPIGMRLGIIRTWDSRWFARKDYPKLLEEDIFIRKYLKK
                     RLSQAGISRIVIQRAVSKVTINISTARPGLVIGRRGQQVDQLRDELQHITKKEIFLNI
                     DEVKKPDIDATLVGEHVARQLEQRVSFRRAMKKAIASTMRAGAGGVRIVCSGRLGGAE
                     MARTESYRDGRVPLHTLRADIDFARSTARTIYGTVGVKVWIFKGEVLDKAVAAEAAAA
                     PSGPARSA"
     gene            2125..2547
                     /gene="rplP"
                     /locus_tag="VFR25_10015"
     CDS             2125..2547
                     /gene="rplP"
                     /locus_tag="VFR25_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9941420.1"
                     /translation="MLVPKRVKHRKQMRGRRKGKAYTGSTVAFGEYGLQALEAAWITN
                     RQIEAARVAITRFIKRGGKMWIRIFPDKPVTVKPAETRMGKGKGAPEFWVAVVKPGRV
                     LFELEGISDDLAKKAMRLGASKLPIKTKFIERHDLAGS"
     gene            2549..2782
                     /gene="rpmC"
                     /locus_tag="VFR25_10020"
     CDS             2549..2782
                     /gene="rpmC"
                     /locus_tag="VFR25_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9941421.1"
                     /translation="MRDLKAEKIRDLNEEEILAKINELTEEIFNLRFRNSMRQLQNPL
                     SIREKRRDIARLRTILTEHRKGIRKLAGHGEAK"
     gene            2815..3048
                     /gene="rpsQ"
                     /locus_tag="VFR25_10025"
     CDS             2815..3048
                     /gene="rpsQ"
                     /locus_tag="VFR25_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9941422.1"
                     /translation="MTGTVVSDKMQKTIVVSIERLVKHATYGKYVRRRNKFKVHDEKN
                     EAKIGDVVRFMETRPQSKDKRWRLLDFVQRVER"
     gene            3067..3435
                     /gene="rplN"
                     /locus_tag="VFR25_10030"
     CDS             3067..3435
                     /gene="rplN"
                     /locus_tag="VFR25_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9941423.1"
                     /translation="MVQPRSIIAISDNSGARVARVVKVLGGTRKRYGRLGDVIVVSIQ
                     DALPGGAVKKGDVHKAVLIRTVKEVRRRDGSYIRFDQNAAVLIDDQKEPKGTRIFGPV
                     ARELREREFMKIISLAPEVI"
     gene            3443..3766
                     /gene="rplX"
                     /locus_tag="VFR25_10035"
     CDS             3443..3766
                     /gene="rplX"
                     /locus_tag="VFR25_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9941424.1"
                     /translation="MKHFRRNDQVIVISGDDRGKSGKILKVMKDVDRVIVEKVNFVKR
                     HTKPRGTGRTGGILEKEAAIHVSNVMHLCPKCQTGVRITVRAGSDGARERYCSRCGET
                     IPRVI"
     gene            3913..4482
                     /gene="rplE"
                     /locus_tag="VFR25_10040"
     CDS             3913..4482
                     /gene="rplE"
                     /locus_tag="VFR25_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9941425.1"
                     /translation="MGEAEAPARVPRLREHYDQNVRAELMKRFNYTNPMQAPRLKKIV
                     VNMGVGDALQNIKMLDTAAAELTTITGQKASIRKAKKSIANFKLRQGQPIGCMVTLRG
                     ARMYEFYDRLVNVALPRIRDFRGVPMRSFDGRGNYTLGLTEQIIFPEINYDRVEKIRG
                     MDVTLVTSARTDEEGQELLRLMNMPFRQR"
     gene            4499..4684
                     /locus_tag="VFR25_10045"
     CDS             4499..4684
                     /locus_tag="VFR25_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9941426.1"
                     /translation="MAKKSIVEKWKRTPKFKVRRYNRCHRCGRPRAFLRDFGICRICF
                     RELALLGQIPGVVKASW"
     gene            4718..5116
                     /gene="rpsH"
                     /locus_tag="VFR25_10050"
     CDS             4718..5116
                     /gene="rpsH"
                     /locus_tag="VFR25_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9941427.1"
                     /translation="MSVSDPVADLLTCIRNACKAKHKKVDVPSSQLKSEIVRVLLREK
                     YINNFKSIDDKKRGILRIYLKYDQKERPVIQGIERMSKPGRRLYIRRHEVPRIQGGLG
                     TALVSTSSGIMTDQEARDEGLGGEYLCRVW"
     gene            5125..5676
                     /gene="rplF"
                     /locus_tag="VFR25_10055"
     CDS             5125..5676
                     /gene="rplF"
                     /locus_tag="VFR25_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9941428.1"
                     /translation="MPSRVGKRPIDIPSGVQVTRSGSAVKVKGPKGEMSFTIAHGVDV
                     AVEPKEILVSQVGGGKQALALHGTTRAVIANMVTGVTQGFSKTLEITGTGYRAQLAGK
                     KLTLQLGYSHPIEFDPPAGITIAVETPTKLTVNGFDKGLVGQVAANIRGFRPPEPYKG
                     KGIRYSGEYIRRKAGKAAGGKSA"
     gene            5707..6081
                     /gene="rplR"
                     /locus_tag="VFR25_10060"
     CDS             5707..6081
                     /gene="rplR"
                     /locus_tag="VFR25_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9941429.1"
                     /translation="MKSKATPRYEGRHRRHLRIRKKVQGSAERPRLTVYRSLNHIYAQ
                     LVDDLAGHTLLSVSSRDEALVGDVKGTKGKVAAGKLVGKALAARAKEKGIVAVCFDRG
                     GYLYHGRVKAVAEGAREGGLTF"
     gene            6090..>6282
                     /locus_tag="VFR25_10065"
     CDS             6090..>6282
                     /locus_tag="VFR25_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011775581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET9941430.1"
                     /translation="MAHPGMRGGRGQQQQDSEFIERVIHVNRVAKVVKGGRRFSFNAI
                     VAVGDGKGKVGVGLGKANEV"
BASE COUNT         1277 a         1938 c         2206 g          861 t
ORIGIN      
        1 catcatcgcg ccgcagctgc tcaaggtagg cgacaagatc ctctcgggcc ccgacgcgga
       61 agtgaagccc gggaactcga tgcccatcgg cgcggtgccg ctggggacgt tcctctacaa
      121 cgtcgagctg gtgcccggaa agggcggcca gctgagccgg agcgcgggca cctactgcca
      181 gctcatggcg aaggaaggcg actacgcgca gcttcggctt ccgtccgggg aaatgcgcaa
      241 ggttccggtc gtctgctcgt gctcgatcgg acaggtcggg aacgaggagc accagaacat
      301 catcatcggc aaggcgggca agtcccgctg gcttggccgg cggcccagcg tccgcggcgt
      361 ggcgatgaac ccggtcgacc acccgatggg cggcggcgag ggcaagtctt cgggcgggcg
      421 gcatccgtgc tcgccgtggg ggcttttggc caagggcaag aagacgcgca agcggaagct
      481 gtcggacgcg ctgatcgtgc gccggcggaa gtcgaagggc gcgtaggcag ctaacgagac
      541 agagggcaac gacgcgatga gccgttccgt caaaaagggt cccttcgtgg acgccaagct
      601 tctgaagcgc ctcgaggcga tgaaccgggt gaacgagcgc cgtgtgctca agacctgggc
      661 acgccgctcg acgatctctc cggagttcgt ggggcacacg ctcgcggtgc acaacggcaa
      721 caagttcatt ccggtctaca tcacggaaaa catggtcgga cacaagctgg gcgagttcgc
      781 ggccacgcgg atcttccgcg gccacggatc gcacgtggag aaagcggccg gacctccggc
      841 ggcgaagtag ccgcccagga cggaacgaca cttatggaag ccaaagcgat cgcgcgatac
      901 gtgcgggtga ccccccgcaa gacggaccag gtgctgcagc tcatccgcgg caagcgggtg
      961 gatcaggcga ccgagatcct cgatttcgcc tcgaagcacg tggccaaggt catcggcaag
     1021 gtgctgaagt cggccgtggc gaacgccgtg gccatcgagg ggaagatcaa catcgaggag
     1081 ctccgggtga agcaggccac ggcgggcgcg ggaccgacga tgaagcggtt ccttccgcgc
     1141 gcgcagggcc gcgccacgcc gctcctcaag cggacgtgcc acatcacgat cgtggtggag
     1201 gggcacgtcg atgaggccgc ccacgcgccg caccgcgcgc gccgcacggg caaggtcgcg
     1261 gcgcccgtcg cgccggccaa ggctgccgtc caggccccgg ccgtcgagac gaaggcggcg
     1321 aaggcccccg cgcggaagaa ggccgcgaag cctgcggcga agaagtcgtc cccggcgaag
     1381 gcgtccgcga agcccgcatc caaggccaag ccgaagaagg gagcgaagta gtggggcaga
     1441 aaaccaatcc gatcggaatg cgcctcggga tcatccggac gtgggactcg cgctggttcg
     1501 cgcgcaagga ctacccgaag cttctcgaag aggacatctt catccgcaag tacctgaaga
     1561 agcgcctgtc gcaggccggg atctcgcgga tcgtgatcca gcgcgccgtc tcgaaggtga
     1621 ccatcaacat ctcgaccgcg cgtccgggcc tggtgatcgg ccggcgcggc cagcaggtgg
     1681 accagcttcg cgacgagctc cagcacatca cgaagaagga gatcttcctc aacatcgacg
     1741 aggtgaagaa gccggacatc gacgcgacgc tcgtgggtga gcacgtggcc cgccagctcg
     1801 agcagcgcgt ctccttccgc cgcgcgatga agaaggcgat cgcctccacc atgcgcgcgg
     1861 gcgccggcgg cgtccgcatc gtctgctcgg gccgcttggg cggcgccgaa atggcgcgca
     1921 ccgagagcta ccgcgacggc cgcgttccgc tccacacgct ccgcgccgac atcgacttcg
     1981 cgcggtccac ggcgcggacg atctacggca ccgtgggcgt gaaggtctgg atcttcaagg
     2041 gcgaggtcct ggacaaggcc gtggcggcgg aagccgcggc ggctccgtcc ggtccggccc
     2101 ggtcggcgta gggagagacc ggaaatgctc gtaccgaagc gggtcaaaca tcggaagcag
     2161 atgcgcggtc gccggaaggg caaggcctac accggctcca ccgtcgcgtt cggcgaatac
     2221 ggtctgcagg cgctcgaggc ggcgtggatc acgaaccgcc agatcgaagc ggcccgcgtc
     2281 gccatcacgc gcttcatcaa gcgcggcgga aagatgtgga tccggatctt cccggacaag
     2341 ccggtgacgg tgaagccggc cgagacgcgt atgggcaagg gcaagggcgc cccggagttc
     2401 tgggtcgccg tggtcaagcc gggacgcgtg ctcttcgagc tggaagggat cagcgacgac
     2461 ctcgccaaga aggccatgcg cctcggggcg agcaagctgc cgatcaagac gaagttcatc
     2521 gagcggcacg atctggcggg gagctgacat gcgcgatctc aaggccgaga agatccggga
     2581 tctgaacgag gaagagatcc tcgccaagat caacgagctg acggaggaga tcttcaatct
     2641 ccggttccgg aactccatgc gccagctcca gaacccgctg tccatccgcg agaagcggcg
     2701 tgacatcgcg cggcttcgga cgattttgac cgagcaccgg aagggcattc gcaagctcgc
     2761 cggacacgga gaggcgaagt gacggagaca acggaacgcg gtaagcggaa ggtcatgacc
     2821 ggcacggtcg tgagcgacaa gatgcagaag acgatcgtgg tttcgatcga gcgtctggtc
     2881 aagcacgcga cctacggcaa gtacgtccgc cgccggaaca agttcaaggt tcacgacgag
     2941 aagaacgagg ccaagatcgg ggacgtcgtc cggttcatgg agacgaggcc ccagagcaag
     3001 gacaaacgct ggcggcttct cgacttcgtc cagcgcgtcg agcgctaggg ggaggaggac
     3061 gacgccatgg ttcagccgcg tagcatcatc gcgatctcgg acaattccgg cgcgcgcgtc
     3121 gcgcgcgtcg tcaaggtgct cggcggcacc cgcaagcggt acggacggct cggcgacgtc
     3181 atcgtcgtct ccatccagga cgcgcttccg ggaggcgccg tgaagaaggg ggacgtccac
     3241 aaggcggtgc tcatccggac cgtcaaggaa gtccgccgcc gggacggaag ctacatccgg
     3301 ttcgaccaga acgcggccgt cctcatcgac gaccaaaagg agcccaaggg aacccgcatc
     3361 ttcggacccg tggcgcgcga gctgcgcgaa cgggagttca tgaagatcat ctccctcgct
     3421 ccggaagtga tctaggggag ccatgaagca ctttcgaagg aacgaccagg tgatcgtgat
     3481 ctccggcgac gaccggggca agtccggcaa gatcctcaag gtcatgaagg atgtcgaccg
     3541 ggtcatcgtc gagaaggtca atttcgtgaa gcggcacacg aagccgcgcg gcacgggccg
     3601 gacgggcggg atcctcgaga aggaggccgc gattcacgtc tcgaatgtga tgcacctctg
     3661 tcccaagtgc cagaccggcg tccgcatcac ggtccgggcg ggctccgatg gagcgcgcga
     3721 acgctactgc tctcgctgtg gcgagacgat tccgagagtg atctgatggc cgacgagaaa
     3781 gagccgaaag acaagaagga agccaagccg aagggcgagg gcaagccgaa gggcgacgcc
     3841 aaggccgccg aggggaaggg cggcaagaag agcaagggca agggcaagga agagaagtcg
     3901 tccgcttcgg cgatcggcga agccgaagcc ccggcccgcg tcccgcggct ccgcgagcac
     3961 tacgaccaga acgtgcgcgc cgagctcatg aagcgcttca attacacgaa ccccatgcag
     4021 gcgccgcgac tgaagaagat cgtggtgaac atgggggtcg gcgacgcgct ccagaacatc
     4081 aagatgctcg acaccgccgc ggccgagctg acgacgatca cgggccagaa ggcctcgatc
     4141 cgcaaggcaa agaagtcgat cgcgaacttc aagctccgtc agggacagcc gatcggctgc
     4201 atggtgacct tgcgcggcgc gcggatgtac gagttctacg atcggctcgt gaacgtcgcg
     4261 cttccgcgaa tccgcgactt ccgcggcgtg ccgatgcgct ccttcgatgg gcgcggcaac
     4321 tacacgctcg ggctcacgga gcagatcatc tttccggaga tcaattacga ccgggtggag
     4381 aagattcgag ggatggacgt cacgctcgtc acctcggccc ggacggacga ggaggggcag
     4441 gagctcttgc ggctcatgaa catgcccttc cggcagcgat agaaagtcgg aggaacgcgt
     4501 ggccaagaag tcgatcgtcg agaagtggaa gcggacgccc aagttcaagg tgcgccggta
     4561 caaccgctgc caccggtgcg gccgtccccg cgcattcctg cgcgatttcg ggatctgccg
     4621 catctgcttc cgggagctcg ccctgctggg gcagattccg ggcgtggtga aggcgagctg
     4681 gtagcacggg caagagcccg gagcgtgaag ggagattatg tcggttagcg atccggttgc
     4741 ggacctgttg acgtgcatcc gcaacgcctg caaggcaaag cacaagaagg tggacgttcc
     4801 gtcctcgcaa ctgaagagcg agatcgtgcg cgtcctcctg cgtgagaagt acatcaacaa
     4861 cttcaagtcg atcgacgaca agaagcgggg catcctccgg atctacctca agtacgacca
     4921 gaaggagcgc cccgtcatcc agggcatcga gcggatgagc aagccgggac gccgcctcta
     4981 catccggcgt cacgaggtgc ccaggattca gggagggctc ggcacggcgc tcgtctcgac
     5041 gtcctcgggg atcatgaccg accaggaggc gcgggacgag ggactcggtg gcgagtacct
     5101 ctgccgcgtc tggtaggagc cgccatgccg tctcgtgtag gtaagaggcc gatcgacatc
     5161 ccgtccggcg tgcaggtcac gcggagcggg tcggcggtca aggtgaaggg ccccaagggc
     5221 gagatgtcgt tcacgatcgc ccacggcgtg gacgtcgcgg tcgagccgaa ggagatcctg
     5281 gtctcccagg tggggggcgg caagcaggcg ctcgcgctcc acggaacgac gcgcgcggtg
     5341 atcgcgaaca tggtcacggg cgtgacccag ggattctcga agaccctcga gatcacgggc
     5401 acgggctacc gggcgcagct cgccgggaag aagctgacgc tccagctcgg ctactcgcac
     5461 ccgatcgagt tcgatcctcc cgcgggcatc acgatcgcgg tggagacccc gacgaagctg
     5521 acggtgaatg gattcgacaa ggggctcgtg gggcaggtcg cggcgaacat ccgcggcttc
     5581 cgtccgcccg agccgtacaa gggcaagggc atcaggtact ccggcgagta catccggcgc
     5641 aaggcgggca aggccgccgg cgggaagtcg gcgtaagccg gcgtccccaa ccgaacggaa
     5701 ggaacgatga aatccaaagc gacgccccgg tacgaaggac ggcatcggcg ccacctgcgc
     5761 attcgcaaga aggtgcaggg ctcggccgag cggccgcgcc tcaccgtgta tcgcagcttg
     5821 aaccacatct acgcgcagct ggtcgacgac ctggccggtc acacgctcct ctcggtctct
     5881 tcgcgcgacg aggctctcgt cggcgacgtg aaggggacca aggggaaggt cgcggccggg
     5941 aagctcgtcg gcaaagcgct cgctgcccgc gccaaggaga aggggatcgt cgcggtctgc
     6001 ttcgaccgcg gcggctacct ctaccatggc cgcgtcaagg cggtcgctga gggcgcccgc
     6061 gagggcggcc tgaccttcta ggagaataca tggctcatcc aggaatgcgc ggcggccgcg
     6121 gccagcagca gcaggattcg gagttcatcg agcgcgtgat ccacgtgaac cgcgtcgcca
     6181 aggtggtcaa gggcggccgg cgcttcagct tcaacgccat cgtcgccgtg ggcgacggca
     6241 aggggaaggt cggcgtgggc ctcggtaagg cgaacgaggt gg
//
LOCUS       DASTKC010000239         5061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_207174, whole genome shotgun sequence.
ACCESSION   DASTKC010000239 DASTKC010000000
VERSION     DASTKC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207174"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VFR25_10070"
     CDS             complement(<1..146)
                     /locus_tag="VFR25_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9941431.1"
                     /translation="MLISERMAAAINTQIGNEFGAFLQYVSIGAHFGSENLPGLSAHF
                     YKQA"
     gene            533..763
                     /locus_tag="VFR25_10075"
     CDS             533..763
                     /locus_tag="VFR25_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941432.1"
                     /translation="MTTATHESDLIAPGATAHPVAPAVADPTDSARGAAPASESERSS
                     DFEVQIAIQAIASRILGAALLERAQEADPIAT"
     gene            783..1322
                     /locus_tag="VFR25_10080"
     CDS             783..1322
                     /locus_tag="VFR25_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9941433.1"
                     /translation="MNDTESIDHAIVQVERLYRSLTGREAPPIREHPYATIPPEKVPE
                     EHVQEQVDRLVETLTEFSGGSSALDGWKPPISMWEGRSEVWISVDLPGVTRDQVRLSV
                     IRGMLELHGTRPPRAATEGSMDLRYTEQTYGKFRRMIPLPSGLALDQLQAQMRSGILE
                     IRVPRATGSAQDVTSIHVG"
     gene            1427..2086
                     /locus_tag="VFR25_10085"
     CDS             1427..2086
                     /locus_tag="VFR25_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941434.1"
                     /translation="MEAKVDVRKLQVLNDRINQTIDALNQVRLSVHGLGHTGVMPQMN
                     PLAYLTQGFGTQGFGAQGLGGQLGGQGFGQPGFGAQGFGGGFPQTTQGIGGLGHTSPF
                     AQADPLSQGNPFQQGNPFQQGNPFQQSSPFQQGNPFQQSNPLSQVNPLSYLTQGLQHS
                     PYSQVNPYGSVPWQSPYGVQGIQGMGRQGYVQDIVDQKLAEIRASDPYRITQTFPFAT
                     M"
     gene            complement(2148..4124)
                     /locus_tag="VFR25_10090"
     CDS             complement(2148..4124)
                     /locus_tag="VFR25_10090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET9941435.1"
                     /translation="MATRDTTTQPTPDDLSPNDERIRYAEVLVEIGDLTLAEMEVAQV
                     LDESPEHLAAMSLFGKLKHMRGQLSLAVACTAQLQSKHLGPGERARMHLESMLHLAQD
                     PTQGAGEFLAMGQFQLVQKPTAYLALEEAFRQYVARRPNEAGTICRTVATRYRDRDSD
                     VYKLAVLAEAWIYELIGDFEVSIQILERLGLERGYETDIDRLLALASLYERTGSRERL
                     ESAVNIGKHLEKNHEDPQVLGRLALLYRRLGDREAATEYERRHLNAYRRKMHRADFRE
                     VVEVASQRFLPIERLRAIHFSDSVLPPGAPEREAALGAAIRGDLATAKRTFSRGEDLL
                     DLKYWANLEALSGTHTGRERAIELYARALKQDPTNLYVIGWLLDREAEAPSPKVADLL
                     KMKSIVPAVLEALEAAVHVSPNDFRIWRRLATLLALQHGGETAQQRRFEERAESLERS
                     SRERARAIGRVLSAATYRFAGTIHGLVHEVWATRELASPGQGGSLRRDDILGNVTEEM
                     RTNVRNTFLATREYAQAKFPHATRDILDYNYGYKVTKEDEPSGGTSAGLPTALAFLSV
                     FLQRPVPQDVASTGVMVTDAHDVLTVRLVGDLDYKVDGAYHRNLRMILVPSGNRPMLE
                     RSTIVPHAISDEIVRYVPDLDQAAGLVFGDLEFL"
     gene            4231..4686
                     /locus_tag="VFR25_10095"
     CDS             4231..4686
                     /locus_tag="VFR25_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941436.1"
                     /translation="MISALVVAAVAYGVVRTMGSKPKKQELDRSPTLEQLDRSIDDVA
                     ATLNGALRARDRVRAGQASAPQARAELDSLRQDLERVAAEVERTQTEPSLVQAYERVS
                     RFAANAARQVGALASDAPADSLERTEARLRAMLTAAGRLRATVAPRPGV"
     gene            4763..>5061
                     /locus_tag="VFR25_10100"
     CDS             4763..>5061
                     /locus_tag="VFR25_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941437.1"
                     /translation="MGHWKKTSLVISGYMAAVLAAVVAGWLYDVRVSALPYDTSGGMY
                     AGGQMLTALAAFFTVALLPTAFLLWSLRRNERFWNTVGILSLWFAIVGLVAVLLPA"
BASE COUNT          865 a         1694 c         1642 g          860 t
ORIGIN      
        1 atcgcctgct tgtagaagtg cgccgacagt ccgggcaggt tctcgctgcc gaagtgggct
       61 ccaatggata cgtactgcag aaacgctccg aattcgttcc caatctgggt gttgatggcc
      121 gcagccatgc gctcgctgat cagcattcgc tctatctccg gtgtcggtcc tccgcgacgg
      181 catccggtac cggatccccc gcgactcgct ctccatgatg gcggacaagt tctgacccag
      241 acgttcgccg aagtcaagta ccgcccctca caccgttgca acagtcacaa tgtacaagcc
      301 cgatcggtac accatgtaac tcttcccacg cgattcatcg catgcagcga ctcaagacat
      361 ttaacagacg catgtgagcg cgtgatggaa tcacgacctg cgggtgaggt acgcgagttg
      421 cgttcgcatc acccttcttg gcctgtcgct tgctgcccct attccacgtc gccgatgagg
      481 cgactcgaac cgacacccgc cactttggac cgagactaaa aggagcgtca tcgtgacgac
      541 ggccacccac gaatccgatc tcatcgcgcc cggcgcgacg gcccatccgg tcgcgccggc
      601 ggtcgcggac cccacggact ctgcgcgcgg agcagcgccg gcatcggagt cggagcgctc
      661 cagtgatttc gaagtccaga tcgcgatcca ggcgatcgct tccaggatcc tcggggccgc
      721 gctcctcgag cgcgcgcagg aggccgatcc cattgccaca tagccgccag gagggacgag
      781 ggatgaacga caccgaaagt atcgaccacg ccatcgtaca ggtggagcgg ctctatcgat
      841 ccttgaccgg tcgtgaggcg ccacccattc gcgagcaccc ttacgccacc atcccgcccg
      901 agaaggtccc ggaagagcac gtgcaggagc aggtcgaccg gctggtcgag accctcacgg
      961 agttctcggg aggctccagc gctctcgacg gttggaagcc gccgatctcg atgtgggaag
     1021 gaaggagcga ggtctggatc agcgtggacc tccccggcgt gacgcgcgat caggtccggc
     1081 tctccgtgat ccggggcatg ctcgagcttc acggcacgcg cccgcctcgc gcggccacgg
     1141 aaggatcgat ggacctccgc tacaccgagc agacctatgg gaagttccgc cgcatgatcc
     1201 cgctcccctc cggcctggcg ctcgaccagc tccaggcgca gatgcgaagc ggaatcctcg
     1261 agatccgggt gccccgcgcg acgggcagcg cccaggatgt gacatccatt cacgtcggat
     1321 aaagaaccag ttgctcgaac cgcctcgacg gcccggtccg cacaggcgct cagcgggggt
     1381 tcggtcgcga ccggggggtc ctaccacgat gaacaaggag gagttcatgg aagccaaggt
     1441 cgacgttcgc aagcttcaag ttctcaacga ccgcatcaat cagaccatcg acgcgctcaa
     1501 tcaggtccgg ttatccgtgc acggacttgg ccacacgggg gtgatgccgc agatgaatcc
     1561 gctcgcctat ctcacgcagg gatttgggac acagggattc ggagcgcagg gccttggcgg
     1621 ccagctcggt ggccagggat tcggccagcc gggctttgga gcgcagggct tcggtggcgg
     1681 attcccccag accacgcaag gcatcggcgg ccttggccac acgagcccct tcgcgcaggc
     1741 cgatccgctc tcgcagggca atccgttcca gcagggcaat ccgttccagc agggcaatcc
     1801 attccagcag agcagcccgt tccagcaggg caatccattc cagcagagca atccgctctc
     1861 gcaggtcaat cccctgtcgt atctgacaca gggtctccag cactcgccat attcgcaggt
     1921 gaatccatac gggagtgtgc cgtggcagag cccgtatggc gtccagggta ttcagggcat
     1981 gggccgacag ggctacgtcc aggacatcgt ggaccagaag ctggccgaga tccgcgcatc
     2041 cgatccgtac cggatcacgc agaccttccc atttgccacg atgtagtctg cagtaagccg
     2101 gtgagcacga gccgaggcgg gcttcgaccc gtctcggctt cgtgtttcta gaggaactcc
     2161 aggtccccga agacgagccc cgccgcctgg tccaggtcgg gcacgtagcg cacgatctcg
     2221 tcgctgatgg cgtgcggcac gatcgtgctc cgctcgagca tcggacggtt gccgctcggg
     2281 accaggatca tcctgagatt tcggtgatag gcgccgtcca ccttgtagtc caggtccccc
     2341 accagccgga cggtgagcac gtcgtgggcg tccgtgacca tgacacccgt ggaagcgacg
     2401 tcctgcggca ccggccgctg caggaagacc gacagaaagg ccagtgccgt cggcaaaccc
     2461 gccgaggtcc ctcccgacgg ctcgtcctcc ttggtcacct tgtaaccata gttgtagtcg
     2521 aggatgtcgc gggtcgcgtg ggggaacttc gcctgcgcgt actcccgcgt cgcaagaaac
     2581 gtgttgcgca cgttcgtccg catctcctcg gtcacgttcc cgaggatgtc gtcgcggcgg
     2641 agagagcccc cctgccccgg cgacgcaagc tcgcgcgtgg cccacacctc gtggaccagt
     2701 ccatgaatcg tgcccgcgaa ccggtacgtg gccgcggaca ggacgcggcc gatcgcgcgg
     2761 gcgcgctccc gcgacgaccg ctccaggctc tcggcccgct cctcgaatcg ccgctgctgc
     2821 gccgtctccc caccgtgctg gagcgcgagg agtgtcgcga gccgccgcca gatccggaag
     2881 tcgttcggcg acacgtgaac cgcggcctcg agcgcttcga gcacggcggg gacgatcgac
     2941 ttcatcttga gcagatcggc caccttgggt gaaggtgcct cggcctcccg gtcgaggagc
     3001 cacccgatca cgtagaggtt ggtcgggtcc tgcttgaggg cgcgcgcgta gagctcgatc
     3061 gcgcgctcgc gccccgtgtg cgtccccgag agcgcctcga gattggccca gtacttgagg
     3121 tcgaggaggt cctcgccgcg ggagaaggtg cgcttcgcgg tggcgagatc gcctcggatc
     3181 gcggccccaa gcgcggcctc gcgctccggc gcgcccggtg ggagcaccga gtccgagaaa
     3241 tggatcgcgc ggaggcgctc gatcggcagg aaccgttgag acgccacctc gaccacctcg
     3301 cggaaatccg cgcgatgcat ctttcgccgg taggcgttca gatggcggcg ctcgtactcg
     3361 gtcgccgcct cccgatcacc cagccgccga tagaggagcg cgagccgccc gagcacctgc
     3421 ggatcttcat gattcttctc gagatgcttc ccgatgttca cagcggactc gagccgctcc
     3481 cggctccccg tccgctcgta gagcgaggcg agcgcaagga ggcggtcgat gtcggtttcg
     3541 tatccgcgct ccaggcccag ccgctccaga atttgaatgg agacttcgaa gtccccgatc
     3601 agctcgtaga tccatgcctc ggcgagcacg gccagcttgt agacgtccga atcgcggtcg
     3661 cggtaccgcg tcgccacggt gcggcagatg gtgccggcct cgttgggacg gcgggccacg
     3721 tactgccgaa atgcttcttc caaagccaga tacgccgtcg gcttctgaac gagctggaac
     3781 tgccccatgg cgaggaattc ccccgcgccc tgagtgggat cctgagcgag gtggagcatc
     3841 gattcgaggt gcatccgcgc gcgctcgccc gggcccaagt gcttcgactg aagctgcgcc
     3901 gtgcacgcga ccgcgagcga gagctgcccc cgcatgtgct tgagctttcc gaagaggctc
     3961 atggcggcga gatgctcagg gctctcgtcg aggacctggg ccacctccat ctcggcgagc
     4021 gtcaggtccc cgatctcgac aaggacctcc gcgtagcgga tgcgctcgtc gttcggcgag
     4081 aggtcgtcag gagtgggctg ggttgtcgtg tctcgcgtgg ccacgattca aggatacccc
     4141 agggcccggg cgttcccctc ccgggtcggc acggggtaca ttggatcgat gcgccagcct
     4201 aggaagagct cgaacggatg gctatgggtc atgatctcgg cgctcgtcgt ggccgcggtt
     4261 gcgtacggag tcgttcgtac catggggtcg aagcccaaga agcaggaact ggaccgctcg
     4321 cccaccctgg agcagctgga tcgatcgatc gacgatgtcg cggcgacctt gaacggggcg
     4381 ctccgggctc gcgaccgggt gcgggcgggc caggcaagcg cgccccaggc gcgggcggag
     4441 ctcgatagct tgaggcagga tctcgagcgg gtggccgcgg aggtagagcg gacacagacc
     4501 gagcccagcc tcgttcaagc ctacgaacgc gtctcgcgct tcgcggccaa cgcggccagg
     4561 caggtcggcg ctctcgcatc ggatgcgccg gcggattccc tcgagcggac tgaggccaga
     4621 ctccgcgcca tgctcaccgc ggccggcagg ctgcgtgcaa ccgtcgctcc cagacccgga
     4681 gtttgaggag gcgctccggc tcaatctccg ctccgtgctt gccgataatg gatgtcgtcg
     4741 aggcgcgctc ggagggggga gcatgggtca ctggaagaag acgagcctgg tgatctcggg
     4801 ctacatggcg gctgtgctgg ccgccgtcgt ggccggatgg ctctatgacg tgcgggtctc
     4861 ggctcttccc tacgacacct cgggcggcat gtacgccggg ggccagatgc tcaccgcgct
     4921 cgcggcgttc ttcacagtcg cgctcctgcc caccgcattc ctgctctggt cgctccgacg
     4981 caacgagcgc ttctggaaca cggtcggaat cctatccctg tggttcgcca tcgtcgggct
     5041 ggtcgccgtg ctcctccctg c
//
LOCUS       DASTKC010000240         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_443184, whole genome shotgun sequence.
ACCESSION   DASTKC010000240 DASTKC010000000
VERSION     DASTKC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443184"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1216
                     /locus_tag="VFR25_10105"
     CDS             95..1216
                     /locus_tag="VFR25_10105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9941438.1"
                     /translation="MKLPLKRPYPPMEAKSVAGIPVGAEWQYEPKWDGFRCVAFRDGD
                     EIALQSKAGEPLERYFPEVVEHLRALSAKRFVLDGELVIPRGKTLSFDDLLMRIHPAE
                     SRVRKLAGETPALYVVFDLLVDDKGVALVEKTLEERRPRLDAFAKAFLTRHEGVALSP
                     VTDDPEVAIEWFQTTRGALDGVIAKRRDLPYQSGERSGMQKIKQRRTADCVVGGFRYA
                     SAGKVIGSLLLGLYDQEGLLHHVGFTSGISTDDRRALVPKLEKLVKPPGFTGHAPGGP
                     SRWSTERSTEWEPLQPKLVAEVEYDHVSGHRFRHGTRFVRWRPDKAPRQCTFEQIAQP
                     EGSLLLLLEGKPSRKAAVPKGKAKVAPRQAAKKKATRTQ"
     gene            1213..>2429
                     /locus_tag="VFR25_10110"
     CDS             1213..>2429
                     /locus_tag="VFR25_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941439.1"
                     /translation="MIRRVLILALASLLSTAMVARAQEAMPDSMAMDHDMSSMHHAHL
                     MAGFYGPYLLNREASGTAWQPENAEHAGIHSMRGDWMLMLHGMADIAYDHQGGPRGDE
                     KFFSSNMLMGMAQRPLGPGTFGAKAMMSIEPATIGKEGYPLLLQTGETADGVDPLIDR
                     QHPHDLFMELALSYSVASGHRSLFVYGGPVGEPALGPPAFMHRFSGSELPLAPITHHW
                     LDSSHITYGVLTGGAVWGGVKAEASAFRGREPDQDRYGIEAPKLDSHSFRLSFNPAPA
                     WAFQASYGRLHSPEQLEPEVDVDRTTVSAMAAGGETDRRWAATLAWGRNRNRPGPILD
                     GVLLEGAASRARHTVFARGEVAEKNELFDHDDPRHGFVYDVGVLEAGYRYDFWSAGPV
                     RTGLGVLGTISFVP"
BASE COUNT          401 a          821 c          828 g          379 t
ORIGIN      
        1 tcgacacggt ccccgcgcgc gtcgcgaagc tgggggacct ctggaagccg cttctcgcgg
       61 cacgcggccg cgtctcgctc gagcgctacc tggaatgaag ctcccgctga agcgccccta
      121 cccgcccatg gaggcgaagt cggtggccgg catcccggtc ggcgccgaat ggcagtacga
      181 gcccaagtgg gacgggtttc gatgcgtcgc gtttcgcgac ggggacgaga tcgcgctcca
      241 gtcgaaggcc ggtgagccgc tcgagcgcta cttcccggag gtcgtcgagc acctgcgcgc
      301 cctcagcgcg aagcgcttcg tcctcgatgg ggagctcgtg attccgcgcg gcaagacgct
      361 ctccttcgac gacctcctca tgcggattca cccggcggag agccgggtga ggaagctcgc
      421 gggagagacg ccggccctct atgtcgtgtt cgatctcctc gtggacgaca agggcgttgc
      481 cctcgtcgag aagacgctcg aggagcgcag gcccaggctc gatgcgttcg cgaaggcctt
      541 cctgacgcgg cacgagggcg tcgcactctc gcccgtcacc gatgatccag aggtcgccat
      601 cgaatggttt caaaccaccc gcggtgcgct ggacggcgtg atcgcgaagc gccgcgacct
      661 gccgtaccag tcgggcgagc gctcgggaat gcagaagatc aagcagcggc gcacggccga
      721 ctgcgttgtc ggcggattcc gctatgcgtc agcgggaaag gtcatcgggt ccctcttgct
      781 cggcctctac gaccaggagg gtctcctgca ccacgtcgga ttcacgtcgg ggatttcgac
      841 ggatgatcgc cgcgcgctcg ttccaaagct cgagaagctc gtcaagccgc ccggattcac
      901 cggccacgcg cctggcggtc ccagccgctg gagcacggag cggtccacag aatgggagcc
      961 gctccagccc aagctggtgg ccgaggtgga atacgaccat gtgagcgggc atcgctttcg
     1021 tcacggcacc cgcttcgtcc gctggaggcc cgacaaggcg ccgcgccagt gcacgttcga
     1081 gcagatcgcg cagcccgagg gctcgctcct cctgctcctc gaagggaagc cgtcccgaaa
     1141 agccgcagtg cctaagggga aggccaaggt cgcgccgcgc caagcggcga agaagaaagc
     1201 gacgcgcacc cagtgatccg ccgggtcctg atcttggcgc tcgcctcgct cctctcgacc
     1261 gcgatggtcg cgcgagcgca ggaagccatg cccgactcca tggccatgga ccacgacatg
     1321 tcttccatgc accacgccca cctcatggcc ggtttctatg gtccctacct cttgaaccgg
     1381 gaagcctccg gcacggcgtg gcagcccgag aacgccgaac acgccgggat ccattccatg
     1441 cggggggact ggatgctcat gctccacggc atggcggaca tcgcgtacga ccatcagggc
     1501 ggcccgcgag gcgacgagaa gttcttcagc tcgaacatgc tcatgggcat ggcgcaacgc
     1561 ccgctcggcc cgggtacgtt cggcgcgaag gccatgatgt cgatcgagcc ggcgaccatc
     1621 gggaaggagg gctacccgct ccttctccag accggggaga ccgcggacgg cgtagatccg
     1681 ctgatcgatc ggcagcaccc gcacgacctc ttcatggagc tggcgctcag ctacagcgta
     1741 gcgagcggcc atcgctcgct cttcgtctac ggcgggccgg tgggggagcc tgcgctcgga
     1801 cctccggcgt tcatgcaccg gttctcggga tccgagcttc cgctcgcgcc catcacgcac
     1861 cactggctcg actcctcaca catcacctac ggcgtgctca cgggaggcgc cgtgtggggc
     1921 ggcgtgaagg ccgaagcctc agccttccgg gggcgggagc ctgatcagga tcgctacggc
     1981 atcgaagcgc cgaagctcga ctcgcactcg ttccgactct cgttcaatcc cgcgcccgcg
     2041 tgggcgtttc aggcaagcta cggtcggttg cacagcccgg agcagctgga gcccgaggtg
     2101 gacgtggatc gcaccacggt ctccgcgatg gcagcggggg gcgaaacgga ccgccggtgg
     2161 gccgcgacgc tcgcgtgggg tcggaaccgg aaccgcccgg gtcccatcct cgacggggtg
     2221 ctgctcgaag gcgccgcatc acgcgcgagg cacacggtct tcgcgcgcgg cgaggtcgcg
     2281 gagaagaacg agctcttcga ccatgacgac ccgcggcacg ggttcgtgta tgacgtgggg
     2341 gtgctcgagg cgggctaccg gtacgacttc tggagcgccg gtccggtgag gacggggctc
     2401 ggagtgctgg ggacgatctc cttcgttcc
//
LOCUS       DASTKC010000241         9239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1625704, whole genome shotgun sequence.
ACCESSION   DASTKC010000241 DASTKC010000000
VERSION     DASTKC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625704"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VFR25_10115"
     CDS             <1..1281
                     /locus_tag="VFR25_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941440.1"
                     /translation="DDVFQAAFTPDGKNLAAIRAVGDLFQLEAPLGTVIYRTKAWMSH
                     PRYSPDGTQIAFLNHPWIGSNGGHVCVVRPGEEPRRLTENIPTMWKLAWRPDGREIWF
                     TGTGDKRHEGILGVTLDGRLRVVYAAPGYLHIEDIAGNGDALLDMMRSQMRIETSTRA
                     DGPAAATDLSWLDWSLLRDLSDDGSLVLFDEAGQVAEFGVFLRTVDGAPAIRLADGVC
                     ASTLSPDGRFVLSRKPGGSDRVHVIPIGVGQARTIATGDCSVIDGDWLPDGRGLIVSG
                     RSPEGRHLYYLDLSSGSLRRLHDTTLKTSMLFVSPDGLRVLARGADSLVTVFPMDGSD
                     PRSYSELTMDWKQCGWQGDSRHFFAYRAGALPAPVVRVDADTGSQEPWMDIMPMVRSG
                     VDSINSVQFDRSAERYACCYISNESHLFHAQGIR"
     gene            1307..1789
                     /locus_tag="VFR25_10120"
     CDS             1307..1789
                     /locus_tag="VFR25_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET9941441.1"
                     /translation="MSMLLRELAGGIPDIDHLVRAIVRIVVSSVLGAVIGIQREHTGK
                     PAGLRTHMLVSMGATLFVLVPVEMGVPIGEVGRVIQGVATGIGFIGGGAILKLSAERE
                     VRGLTTAAGIWMTAAAGVAVGLGYIAVAALGIGAAWLILSVVGRAERRVEAKPKSYER
                     "
     gene            complement(1790..3079)
                     /locus_tag="VFR25_10125"
     CDS             complement(1790..3079)
                     /locus_tag="VFR25_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9941442.1"
                     /translation="MPRFSRRSEQIPFSPIRTMFKLADEMERAGGGPVFRLHVGDPDF
                     TPPPRVVEASCDALKKGKTHYAPSAGIEDLRAALAEKARTRNGIAATTEQVIVTPGST
                     QALFASMELMLGAGDQMLLPAIYWPNYVQQVLLIGALPVFYPLGRGYQPDLDALRKHA
                     SKKLRAIMINSPSNPTGAVFPEATLRALYDFARERDLWVLSDEAYEDYIFGGAHVSPA
                     SFERDLPEDERRVFTLFTFSKSYAMTGLRVACIVAPSTRAGTMLRKVQEPLVASSGTP
                     MQWGALAALDLGERAGIERMRETYRRRRDLAMSILGPAGLADYEPEGAFYIMADVSTT
                     GLTGDEFALRLLSEERVAVAPGSGFALVPEYAPDGTLMDEPTEAGAPEFATHPGARYR
                     VRIAFCVSDEELREGLERMVRMVGRLRRGSPTPAAAR"
     gene            3284..3784
                     /locus_tag="VFR25_10130"
     CDS             3284..3784
                     /locus_tag="VFR25_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941443.1"
                     /translation="MRRSRTALLVVAALTVAVVTAAVAQDKAAGTATKTTTTAKTATT
                     AKASSSSSTKGSPSSGKLGVKTIAGDIIDPSCWVMNGAKGEAHKECMVACAKAGQTLA
                     ILEKKTNKVYILAAERPGEDPNKSVLDYAGMSVLVKGKVFTRGGIMAIQVASVEPYSA
                     AKAAAK"
     gene            3812..4348
                     /locus_tag="VFR25_10135"
     CDS             3812..4348
                     /locus_tag="VFR25_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941444.1"
                     /translation="MTSRTTSLGLAVALVAAVAVLGAGEAQAPKSTTKPATPAPAAAH
                     TGHEGHDMSAMKPAKPKPQGERKTITGEVMDPACFLEAGPKSIGTGHFQCAVDCVRSG
                     QTLAIYDRDADRIYFVAGELPGRNPNEPLLPYIHKKVDVTGVVYHRSDAYGIVISKVT
                     PHEDKGGASPAPAAGTTR"
     gene            4380..6239
                     /locus_tag="VFR25_10140"
     CDS             4380..6239
                     /locus_tag="VFR25_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HET9941445.1"
                     /translation="MNSLVLPLLAIVVFSFAYRYYSAFMAYRVARLDPLRAVPSVTQA
                     DGRDFVKTNKFVLFGHHFAAISASGPLIGPVLAAQFGYLPGALWILFGAALGGAVHDF
                     IILFASVRHGASSLSEIVRREIGSVAHGAATAAILFITILLLAGLGIVVVNALAGSPW
                     GTFTVAMTIPAALIFGWYMYRLRPGRVVEGSLIGVALILAAVIAGPWVVKSSIAHWFT
                     LSPHSLHLILPIYAFIAATLPVWLLLCPRDYLSTYMKIGTILLLVLGVAIVHPKLLMP
                     AITPYIDGSGPVLPGMKVFPFCFIVIACGAISGFHALIGSGTTPRMVPDEPSIRFIGY
                     GAMLTEGLVAIMALVAATALAPADYFLINGVPKAVAALGIQPHQLQELTRLVGEETLQ
                     GRTGGAVSLAVGMANILRQIPGMGNLMAYWYHFAIMFEAVFILTALDTGTRVARYLFQ
                     DVAGKVWAPLGSTTNVVSVAFVGAVVCFCWGYMMATGSVETIWPLFGVSNQLLAVIAL
                     AAGTTFILRQRPPAYALTTFLPLCFLTVTTMTAGVLNTLRYLSPSYIADKGPFVGYLD
                     GILSIVLLLLVATVLLDSVRRWTVILAARRLGAAEIPMAHVAELGAPAEPPLA"
     gene            6241..8736
                     /locus_tag="VFR25_10145"
     CDS             6241..8736
                     /locus_tag="VFR25_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941446.1"
                     /translation="MTIAPGTRLGPYEIVGRVGEGGMGVVYRARDDRLGRDVAVKVIP
                     PELASDKERLRRFQIEARAAGQLNHPNIVIIHDLGTSDGAPYIVSEMLEGETLRPSLS
                     RGPLPARRAVSLAVQIAHGLASAHAAGIVHRDLKPENLFLNRSGVLKILDFGIAKLFQ
                     SDGAAGEETASVLPGLTRTGTILGTASYMAPEQIREQAIDQRTDLFALGAILYEMLTG
                     EKAFPGATPADRMSAILNRDTPPLPREIEAEVPGIGRIVSRCLEKLPEHRFQTASDLG
                     FALELLEEFQPSGATPSQPVVPAGPAIQFPRYERVTYREGNVRTARFAPDGQSVIYGA
                     ALEGTPLRLYWSYPGSPEARAIGVPGSDLMSVCASTSELAVSLRTVFLGGFVSAGTLA
                     RMPLGGGAARELVDEVVEADWAPDGRNLSIIREKHGMIRLEYPVGRVLYQTPGWASHA
                     RIAPDGKSIAFIDHPFRGDDAGRVAVVDMEGNVRQLVGDFASTRGLAWTPKGDEIWFS
                     GAKVGADRVVHAVRTDGTTRQVFSAPGASDVQDISRSGTLLIAQGDERLRMEYRNRAG
                     DVMRDLTWLDWSLVRDLSSDARSVLFDETGAGGGSGQSVYMRDVDGSPAVRLGDGSAV
                     QFSPDGRWALALQIQNTRAVLLPTGVGEPRPIETEGLLVRWGAWHPDGKSLLVTGNEP
                     GQGSGLYRVDVETARWTRIGDRELSGFQEVRVSPDGSRVVAQGADGEFTIFPMDGSEP
                     RSLPGIPSRARIIGWTRDGGALLYMIRGEVPAPTFRIDVETGEKTHWVDLAPKSLAGV
                     VTLTRIVMTPDAENFVYSYPRFLTNIYKVEGLL"
     gene            8778..>9239
                     /locus_tag="VFR25_10150"
     CDS             8778..>9239
                     /locus_tag="VFR25_10150"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HET9941447.1"
                     /translation="MYEASKLLHIFAVVLFLGNITGGIFWKLFGDKTRDPRIIAHTMA
                     GIIASDRLFTNPGAILILIGGITAALLGHYPILRTHWISSSIGLFALSGAAFGIRLVP
                     LQRQLLAVARAGVEGTMDWDRYDKLSGQWKLWGMVALVAPLLAMVLMVLKPG"
BASE COUNT         1435 a         3139 c         3133 g         1532 t
ORIGIN      
        1 gacgacgtgt tccaggcagc cttcactccg gatggcaaga atctcgcggc gatccgtgcg
       61 gtcggcgatt tgtttcagct cgaggcgccg ctcggcaccg tgatctatag gacgaaggcc
      121 tggatgagcc acccgcgcta ctcacccgat gggacgcaga tcgcgttcct gaatcatccc
      181 tggatcggga gcaacggggg acacgtctgc gtcgttcgac ccggtgagga gccgcgccgg
      241 ctcaccgaga acatcccgac gatgtggaag ctcgcgtggc ggccggatgg acgggagatc
      301 tggttcaccg gcacggggga caagcgccat gaggggattc tcggtgtcac cctcgacgga
      361 cgcctccggg tcgtctacgc ggcgcctgga tacctgcaca tcgaggacat cgcgggaaac
      421 ggggacgcgc tcctcgacat gatgcgctcg caaatgcgca tcgagacatc cacgcgggcg
      481 gacggcccgg ccgccgcgac ggatctgtcc tggttggatt ggtcgctgct tcgcgatctg
      541 agtgacgacg gatcgctcgt gctgttcgat gaggccggcc aagtcgccga gttcggcgtt
      601 ttcctccgaa cggtcgacgg agcgccggcg atccgtcttg ccgacggcgt ctgcgcatcc
      661 accctctctc cagacgggcg cttcgttctg agccgaaaac caggcggctc ggatcgggtc
      721 catgtgattc cgatcggagt gggacaggca cggacgattg ccaccggcga ttgctcggtc
      781 atcgacggcg actggctccc cgacgggcgc gggctgatcg tgagcgggag gtcgcccgag
      841 gggcggcatc tctattacct cgacctgagc tcggggtcgc ttcgtcgcct ccacgacacg
      901 acactcaaga cctcgatgct cttcgtgtct cccgacggat tgcgcgtcct cgcgcgtggc
      961 gcggactcgt tggtcacggt cttcccgatg gacgggtccg atccccgatc ctattccgag
     1021 ctcacgatgg actggaagca gtgcggatgg cagggcgatt cacgccactt cttcgcctac
     1081 cgcgcgggcg cgctgcccgc gccggtcgtg cgcgtcgacg cagacacggg atcgcaggag
     1141 ccatggatgg acatcatgcc catggtgcga agcggagtcg acagcatcaa ctcggttcaa
     1201 ttcgaccgca gcgccgagcg ttacgcctgc tgctacatct cgaacgaatc gcacctcttc
     1261 catgctcagg ggataaggtg acggaggcac gaaaggagca cacggcatga gcatgctgct
     1321 tcgggagctg gccggcggca tccccgatat cgatcacctg gtgcgcgcga tcgtccgcat
     1381 cgtggtctcg tccgtcctcg gcgccgtcat cggaatccag cgggagcaca ccgggaagcc
     1441 ggcgggcctt cgcacgcaca tgctcgtatc gatgggggcg acgctcttcg tgctcgtccc
     1501 ggtcgagatg ggggtcccca tcggcgaggt gggccgcgtg atccagggcg tcgcgactgg
     1561 gatcggcttc atcggcggcg gcgccatcct caagctctcc gcggaacgcg aagtgcgagg
     1621 cctaacgacc gcggccggga tctggatgac ggcggcggcg ggagtggcgg tcggcctggg
     1681 ctacatcgcg gtcgccgcgc tcgggatcgg cgctgcgtgg ctcatcctct cggtggtggg
     1741 acgcgcagag aggcgcgtcg aggcgaagcc gaagagctac gagcgctagc tagcgggcgg
     1801 cggccggagt gggcgatcca cggcgcagcc gtcccaccat tcgcaccatc cgctccaggc
     1861 cttcgcgcaa ctcttcatcc gacacgcaga acgcgatgcg gacgcggtag cgggcgcctg
     1921 ggtgcgtcgc gaactcaggc gctcctgcct ccgtgggttc gtccatcagg gttccatcgg
     1981 gcgcatattc cggaaccagc gcgaagccgg aacccggggc gaccgccacg cgctcctcac
     2041 ttagaagacg cagcgcgaac tcgtcccccg tgagtcccgt cgtcgagacg tccgccatga
     2101 tgtagaaagc cccttccggc tcgtaatccg cgagccccgc cgggccgagg atcgacatgg
     2161 cgagatctcg ccggcgccgg tacgtctcgc gcatccgctc gatcccggca cgctcgccaa
     2221 ggtcgagagc ggcgagcgct ccccactgca tcggcgtgcc cgagctcgcg acgagcggct
     2281 cctggacctt ccggagcatc gtcccggcgc gcgtcgacgg cgcgacgatg cacgccacac
     2341 ggaggcccgt catcgcgtac gacttggaga acgtgaagag cgtgaagacg cgccgctcgt
     2401 cctcggggag atcccgctcg aaggacgccg gcgacacgtg cgcaccgccg aagatgtagt
     2461 cctcataggc ttcgtcgctc aggacccaca ggtcgcgctc gcgcgcgaag tcgtagaggg
     2521 cgcggagcgt ggcctcgggg aacacggcgc ccgtcgggtt gctcgggctg ttgatcatga
     2581 tcgcgcggag cttcttgctg gcgtgcttcc ggagcgcgtc gaggtcgggc tggtagccgc
     2641 gcccgagcgg gtagaagacc ggaagggctc cgatcaggag cacttgctgc acgtagttgg
     2701 gccagtagat cgcggggagc agcatctggt cccccgcgcc gagcatgagc tccatggaag
     2761 cgaagagggc ctgcgtggac ccgggagtga cgatgacctg ctcggtggtt gccgcgatcc
     2821 cgttccgcgt gcgggccttc tcggcgaggg ccgcgcggag atcctcgatt cctgcgctcg
     2881 gggcgtagtg ggtctttccc ttcttgagcg catcgcacga ggcctcgacc acgcgcggag
     2941 ggggcgtgaa gtcggggtcg cccacgtgga gccggaatac cggtccgccg cccgcccgct
     3001 ccatctcgtc cgccagtttg aacatggtcc ggatggggga gaaggggatc tgctcgctcc
     3061 ggcgggaaaa gcgcggcatg ggcacctcgg tcgtggacgt ggactccgag ctgggaaacg
     3121 cccggatgat accagagcgc cggatgcccg ccagggcgga ccctcctcgc ccgctccggt
     3181 gtagccatag gcacgctcgc gggttgcccg gacgggcgcc gctctggtag ccttgcggca
     3241 accgggcccc ggttcatcca ggtaaattca agggaggcaa ggaatgcgac ggtccagaac
     3301 cgcgcttttg gtcgtggcgg cgctcacggt ggcggtggtg acggcggcgg tggcgcagga
     3361 caaggcggcc ggtacggcga ccaagacgac gaccacggcc aagacggcga ccacggccaa
     3421 ggcatcctcg tcctcttcca cgaagggctc gccctcatcg ggcaagctcg gcgtgaagac
     3481 gattgccggc gacatcatcg atccctcctg ctgggtgatg aacggcgcca agggcgaagc
     3541 gcacaaggag tgcatggtgg cctgcgccaa ggcggggcag acgctcgcca tcctcgagaa
     3601 gaagacgaac aaggtctaca tcctggccgc tgagcggccg ggcgaagatc ccaacaagag
     3661 cgtgctcgac tacgcgggca tgtcggtact ggtcaagggc aaggtcttca cgcgaggcgg
     3721 catcatggcc atccaggtcg cctcggtcga gccgtattcg gccgccaaag cggcggcgaa
     3781 gtagacgcat cggggcgcga aggagcaagg catgacttcc agaacgacat cactcggact
     3841 cgcggtggca ctcgtcgcgg cggtcgccgt gctcggcgcc ggcgaagcgc aggccccgaa
     3901 gtccacgacg aagcccgcca cccccgcgcc ggccgcggcg cataccggac atgaagggca
     3961 cgacatgtcc gcgatgaagc cggccaagcc caagccccaa ggcgagcgga agacgatcac
     4021 gggcgaagtc atggatcccg cctgcttcct cgaggcaggg ccgaagtcga tcggaaccgg
     4081 gcatttccag tgcgccgtcg actgcgtgcg ctccgggcag accctcgcca tctacgatcg
     4141 cgacgccgac cggatctatt tcgtggccgg agagctcccg ggacggaacc ccaacgaacc
     4201 gctcctgccg tacatccaca agaaggtgga cgtcacgggc gttgtctatc accgctccga
     4261 cgcgtacggc atcgtgatct cgaaggtgac gccgcacgag gacaagggcg gcgcgtctcc
     4321 ggctccggcg gcggggacca cccgctagcg acccgacatc cggggaggga cgcacccgcg
     4381 tgaattcgct cgtcctgccg ctcctcgcga tcgtcgtctt ttcgttcgcc taccgctact
     4441 acagcgcgtt catggcgtac cgtgtggcgc gcctcgatcc gctccgcgcc gttccgtccg
     4501 tcacgcaggc cgacggacgc gacttcgtga agacgaacaa gttcgtcctc ttcggacatc
     4561 acttcgcggc gatctcggcg tccggacctc tgatcggccc cgtgctcgcc gctcagttcg
     4621 gctacctccc cggcgccctc tggatcctct tcggcgccgc gctcggcggg gccgtccacg
     4681 atttcatcat cctgttcgcg tccgtgcgcc acggcgcgtc gtcgctctcg gagatcgtgc
     4741 gccgggagat cggctccgtc gcccacggcg ccgcgaccgc ggccatcctc ttcatcacga
     4801 tcctcctcct cgcggggctc ggcatcgtcg tcgtgaacgc gctcgcgggc tcgccctggg
     4861 gcaccttcac cgtggcgatg accatcccgg cggcgctcat tttcggctgg tacatgtacc
     4921 gcctccgccc cggacgcgtg gtggagggaa gcctcatcgg ggtggcgctc atcctggctg
     4981 cggtgatcgc gggcccgtgg gtcgtgaagt cctcgatcgc ccactggttc acgctctcac
     5041 cccactcgct ccacctgatc ctgccgatct acgcattcat cgcggcgacg ctcccggtct
     5101 ggctgctcct ctgtccgcgc gactatctct cgacctacat gaagatcggc accatcctgc
     5161 tcctcgtcct cggtgtcgcg atcgtccatc ccaagctcct gatgccggcg atcacgccct
     5221 acatcgacgg gtcaggaccc gtcctcccgg ggatgaaggt ctttccgttc tgcttcatcg
     5281 tgatcgcgtg cggcgcgatc agcggcttcc acgcgctgat cggatcgggg acgacgccgc
     5341 ggatggtccc ggacgagccg agcatccgct tcatcggcta cggcgccatg ctcacggagg
     5401 ggctcgtcgc gatcatggcg ctggtggcgg ccaccgcgct cgcccccgcc gactacttcc
     5461 tcatcaatgg cgtgccgaag gcggtggccg cgctcggcat ccagccgcac cagctccagg
     5521 agctcacgcg gctcgtcggc gaggagacgc tccaggggcg caccggcggc gcggtgtcgc
     5581 tcgccgtggg catggccaac atccttcggc agattcccgg catgggcaat ctcatggcgt
     5641 actggtacca cttcgcgatc atgttcgagg cggtcttcat cctgaccgcg ctcgataccg
     5701 gcacccgcgt cgcgcgttat ctcttccagg acgtcgccgg caaggtctgg gcgccgctcg
     5761 gctccaccac caacgtcgtg agcgtggcgt tcgtgggcgc ggtcgtctgc ttctgctggg
     5821 ggtacatgat ggcgaccggg agcgtggaga cgatctggcc cctattcggc gtctcgaacc
     5881 agcttctggc ggtgatcgcc ctggccgccg ggacgacgtt catcctgcgt caacgcccgc
     5941 cggcgtacgc gctcaccacg ttcctcccgc tctgtttcct caccgtgacc acgatgaccg
     6001 ccggagtgct caacacgctc cgctatctct cgccgtccta catcgcggac aaagggccgt
     6061 tcgtcggata cctcgacgga atcctgagca tcgtgctcct cctcctcgtg gccacggtcc
     6121 tcctggactc ggtccgccgg tggaccgtga ttctcgcggc ccgccgtctg ggtgcagccg
     6181 agatcccgat ggcccacgtc gctgagctcg gcgctcccgc cgagccgccg ctcgcctgag
     6241 atgaccatcg ccccggggac gcggctcgga ccctacgaga tcgtggggcg cgtgggcgag
     6301 gggggcatgg gcgtcgtcta ccgagcccgc gacgaccggc tggggcgcga cgtcgcggtg
     6361 aaggtgatcc cgcccgagct cgcctccgac aaggagcgcc tccgccgctt ccagatcgaa
     6421 gcccgcgcgg ccgggcagct caaccatccc aacatcgtca tcatccacga tctcggcacg
     6481 tcggacggcg cgccctacat cgtgtcggag atgctcgaag gggagacgct gcgtccgtcg
     6541 ctctcgcgcg gcccgctgcc ggcacggcgc gcggtctcgc tcgcggtgca gatcgctcat
     6601 ggcctggcgt ccgcgcacgc ggcggggatc gttcatcgcg atctcaagcc cgagaacctg
     6661 ttcctgaacc ggagtggcgt tctcaagatc ctcgacttcg gcatcgcgaa gctcttccag
     6721 tcggacggcg ccgcgggcga ggagaccgcg agcgtgctgc ccgggctcac gcgcaccggg
     6781 acgattctcg gcacggcgag ctacatggcg ccggagcaga ttcgcgagca ggcgatcgat
     6841 cagcgcacgg acctctttgc cttgggcgcg atcctctacg agatgctgac gggcgagaag
     6901 gcgttccccg gcgcgacgcc ggcggatcgc atgagcgcga tcttgaatcg cgacacgccg
     6961 cccctcccgc gggagatcga ggcggaggtt cccggcatcg gccgcatcgt gtcgcggtgc
     7021 ctggagaagc tgcctgagca tcgattccag accgcgagcg atctcggatt cgcgctcgag
     7081 ctgctcgagg agttccagcc ctccggcgcg accccgtcgc agcccgtcgt gcccgccgga
     7141 ccggccattc aattcccgcg ctacgagcgg gtgacctacc gcgagggaaa cgtgcgcacc
     7201 gcgcgcttcg ctccggacgg acagtcggtg atctacggcg cggcgctcga gggcaccccg
     7261 ctccgcctct attggtctta tccgggaagt ccggaagcgc gcgccatcgg cgttcccggc
     7321 agcgatctca tgtccgtgtg cgcctcgacc agcgagctgg ccgtcagcct gcgcacggtg
     7381 ttcctcggag gcttcgtgtc cgcggggacc ctggcgcgca tgccgctcgg cggcggcgcc
     7441 gcccgcgagc tggtcgacga agtggtcgag gcggactggg cgccggacgg gcggaacctc
     7501 tcgatcatcc gcgagaagca cgggatgatc cggctcgagt atccggtcgg acgggttctc
     7561 taccagacgc cgggatgggc cagccacgcc cggatcgctc ccgacggcaa gtcgatcgcg
     7621 ttcatcgatc atccatttcg aggcgacgat gcgggccgtg tcgccgtcgt cgacatggaa
     7681 ggcaacgtgc ggcagctcgt gggggacttc gcctccacac gcggcctcgc gtggacgccg
     7741 aagggcgatg agatctggtt ctccggagcc aaggtgggcg cggatcgcgt cgtccatgcg
     7801 gtccggacgg acgggacgac acggcaggtc ttcagcgcgc caggcgcgag cgacgttcag
     7861 gacatctctc gaagcggcac cctgctcatc gcgcaagggg acgaacggct tcggatggag
     7921 taccggaatc gcgctgggga cgtgatgcgg gacctcacct ggctggactg gtcgctggtg
     7981 cgggatctca gttcggacgc ccgcagcgtg ctcttcgacg agaccggcgc cggaggaggc
     8041 tccgggcagt cggtctacat gcgcgatgtg gacggatcgc ccgccgtgcg cctcggcgac
     8101 ggctcggccg tgcagttctc tcccgacggg cgctgggcgc tggcgcttca gattcaaaac
     8161 acgcgggccg tcctccttcc cacgggagtg ggggagccgc ggccgatcga gacggaaggg
     8221 ctactcgtgc gctggggagc ctggcacccg gacgggaaga gcctgctcgt gacggggaac
     8281 gagcccgggc aaggatcggg actctatcgc gtagacgtcg agaccgcccg gtggacccgg
     8341 atcggggacc gggagctgag cggattccag gaagtgcgcg tctctcccga tggaagccgc
     8401 gtggtcgcgc agggtgcgga cggtgaattc acgatctttc cgatggatgg aagcgagccc
     8461 cgttcgcttc cgggcatacc gagccgcgcg cggatcatcg ggtggacccg ggacggcgga
     8521 gccctcctct acatgatccg cggcgaggtt ccggctccca cgttccgcat cgacgtcgag
     8581 accggcgaga agacgcactg ggtcgatctc gcgcccaaat cgctggcggg ggtcgtgacg
     8641 ctcacgcgca tcgtgatgac gcccgacgcg gagaatttcg tctacagcta ccctcgcttc
     8701 ctcaccaaca tctacaaggt ggaagggctc ctctagccgg cgcgagccgg ccttcgctcc
     8761 gaacccacgg agggggcatg tacgaagcct cgaagctgct gcacatcttc gcggtcgttc
     8821 tcttcctcgg caacatcacg ggcggcatct tctggaagct gttcggagac aagacgcggg
     8881 atccgcgcat catcgcgcac acgatggcgg gcatcatcgc gagcgaccgc ctcttcacga
     8941 atcccggcgc gatcctgatc ctgatcggcg ggatcacggc cgcgctcctg ggacactatc
     9001 caatcctacg gacccactgg atctcctcgt cgatcggtct ctttgccctc tccggcgcgg
     9061 cgttcggcat ccggctggtt ccgctccaga ggcagctgct cgcggtcgcg cgggccggcg
     9121 tcgaggggac catggactgg gatcgctatg acaagctctc cgggcagtgg aagctctggg
     9181 ggatggtcgc gctcgtggcg ccgcttctcg cgatggtgct catggtgctg aagccgggc
//
LOCUS       DASTKC010000242         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1864343, whole genome shotgun sequence.
ACCESSION   DASTKC010000242 DASTKC010000000
VERSION     DASTKC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864343"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFR25_10155"
     CDS             complement(<1..624)
                     /locus_tag="VFR25_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9941448.1"
                     /translation="MPQALIVDDEPTSVSAMAELVEKEGFTTTTAGTLADAKARLSQE
                     RPDVILVDLMLPDGNGLELLEEMDPSRRAEVILISGHATVDSAISALRVGVLDYLTKP
                     VDVRRLKAVLANVSRTLAFKEEVGSLREELRQLGHFGRLIGNSTPMQRVYDLITRVAP
                     TDATVLLVGESGTGKEEVAATVHELGRRKKQPFLPLNCGAVPPNLIES"
     gene            complement(633..1322)
                     /locus_tag="VFR25_10160"
     CDS             complement(633..1322)
                     /locus_tag="VFR25_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9941449.1"
                     /translation="MPADPESKLAEHVRRLSRLFRAAGHDLRGPLHSMALQLELLRQS
                     TEEVEGAELRARQERYATAAAKEISRLARMLESLWGGAEDGDQRVPFDLRETTEEIRA
                     MAEPHFRRERIALAFETPDTAVEAEGSRAAVRHAGLDLLLHAAERVPAGKVTLTVETR
                     DHAAVLVVAATARDGADVRESRDETTEEAPFAADRIARRFGGTVRATRPGQASWRWEL
                     ELPRPRTPESE"
     gene            1763..2071
                     /locus_tag="VFR25_10165"
     CDS             1763..2071
                     /locus_tag="VFR25_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9941450.1"
                     /translation="MHELQDTYGTPRTSKSINPQFMVGALLGAGVALLLAPANGKDTR
                     KKVGVAAQKLGANAKNVVGRTRHFVEGIKQDAQSAMKSGRDEFVRNQRSRDTGAAWTA
                     "
     gene            2240..>2396
                     /locus_tag="VFR25_10170"
     CDS             2240..>2396
                     /locus_tag="VFR25_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HET9941451.1"
                     /translation="MDSFDKLYLDQLKDAYNAEKQLVKAIPKMVKAATSPELRTALED
                     HLEVTRRQ"
BASE COUNT          403 a          793 c          808 g          392 t
ORIGIN      
        1 gctctcgatg agattgggcg gaacggctcc gcagttgagc ggcaggaacg gctgtttctt
       61 ccgccgcccc agctcgtgga ccgtggccgc gacctcctcc ttgccggtgc cgctctcacc
      121 cacgagaagg accgtggcgt cggtgggagc gacgcgtgtg atgaggtcgt acacgcgctg
      181 catgggcgtg gagtttccaa tcaggcggcc gaagtgcccg agctggcgca gctcctcgcg
      241 cagcgatccg acctcctcct tgaaggccag cgtccgcgag acgttggcga gcacggcctt
      301 gaggcggcgc acgtccaccg gcttcgtgag gtagtcgaga acgcctacgc gaagcgcgga
      361 gatggcggaa tcgacggtgg cgtgtcccga gatcaggatc acctcggcgc gacgcgacgg
      421 atccatctcc tcgagaagct cgagaccgtt gccgtcggga agcatgagat cgaccaggat
      481 cacgtccggg cgctcctggc tgaggcgagc cttcgcgtcc gccagggtcc ccgcggtcgt
      541 ggtcgtgaag ccttccttct ccaccagctc ggccatggcg ctgaccgagg tgggctcgtc
      601 gtcgacgatg agtgcctgag gcatgactct ccttactccg attcgggggt tcggggacga
      661 ggaagctcga gctcccatcg ccaggacgcc tggccgggtc tcgtggcgcg aacggtcccg
      721 ccgaaacggc gggcaatacg gtccgcggcg aatggtgctt cctcggtggt ctcgtcgcga
      781 ctctcgcgca catccgcgcc gtcccgcgcc gtggcggcga ctacgagcac ggccgcgtga
      841 tcccgggtct ccacggtcag ggtgaccttg ccggccggaa cgcgctccgc cgcgtgcagg
      901 aggagatcga gtcccgcgtg gcgtacggcc gcgcggctcc cctccgcttc cacggccgtg
      961 tccggggttt cgaacgcgag cgcgatccgc tcgcgccgga agtgtggctc ggccatcgcg
     1021 cggatctcct ccgtggtttc acggaggtcg aagggcacgc gctggtctcc atcctcggcg
     1081 cccccccaga gcgactcgag catgcgcgcc aggcgcgaga tctccttggc cgccgcggtc
     1141 gcgtagcgct cctgacgcgc acgaagctcg gcgccctcca cctcctccgt gctctgacgc
     1201 aggagctcga gctggagcgc catcgaatgg agcggccctc tcaggtcgtg ccccgcggcc
     1261 cggaagagcc gggagagccg gcgcacgtgc tcggccagct tcgattccgg atccgctggc
     1321 accagagaac cctccgtcat ggcgaggaaa ggagcttcga acgggatggc tgattcggtc
     1381 atctctcgag cgtcctggtt gcggtccatg gaacgatgat cggccccggc gatcggttgc
     1441 accaaggcac ctatcgagca tggcacgctc gcgaagccgg ggacaagggg ccctttgcaa
     1501 tttttttcat ggcgctcgcg aagcggccaa caactcggcg ccgcttcagg ttcccgatct
     1561 tgtctcgatc gtctcgcgcg tggtggcccc gtacttgctc gacggaatgt acgagctggg
     1621 gggctcgacg gggtcactgc aagcgaccct gacgtaacgc gcgctcgaac ttgggacgtc
     1681 cccatttctc ccattgggac gcccgttgta acaacgtcgg gcacgccatc gagcatcgta
     1741 tgacgaacca aaggagacga tcatgcacga gctccaggac acctacggaa caccacgcac
     1801 aagcaagtcc attaatcctc agttcatggt cggcgctctc ctgggcgccg gcgttgcgct
     1861 tctcctggcc ccggccaacg ggaaggacac gcgcaagaag gtgggagtgg cggcccagaa
     1921 gctgggagcg aacgccaaga acgtcgtcgg gcgcacgcgc cacttcgtcg aggggatcaa
     1981 gcaggacgcg caatcggcca tgaagtccgg gcgcgacgag ttcgtgcgga atcagcggag
     2041 ccgcgacacg ggcgccgcct ggaccgcgta gcacgactcc gcgggatccg gctcggagga
     2101 ggtccccctt caaaccggcg ccgacaccga tcccgcgcga gttgatttcg aggaggccgg
     2161 gcagggctcg tcgaggagcc gcccggacct ccgctctttt gtgcatgtga agcgtgagcc
     2221 aaggagggat gccatgcata tggattcgtt cgacaagctc tacctggacc agctgaagga
     2281 cgcttacaac gcggagaagc agctggtgaa agccattccg aagatggtca aggccgcgac
     2341 atcgcccgag cttcgcaccg cgctggagga tcacctcgag gtgacccgcc gccagg
//
LOCUS       DASTKC010000243        31587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_919510, whole genome shotgun sequence.
ACCESSION   DASTKC010000243 DASTKC010000000
VERSION     DASTKC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 31587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31587
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919510"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFR25_10175"
     CDS             <1..1363
                     /locus_tag="VFR25_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET9941452.1"
                     /translation="LATADFSASSASVLLGNGDGTFQPKVDYGTWTNPTAIAIGDVSA
                     DGKPDLVTTGSSLGFNMVSVLLNNGDGTFQTKVDYEVGTLPWAVAIGDVSGDSKSDIV
                     TANYFGGTLSVLLGNGDGTFQASVDYGTADSPISVAIRDVSGDGKPDLIAAANGGVSV
                     HLGNGDGTFQPKTDYPGVSAYSVAIGDVSLDGELDIVTANGTNTVSAFLGNGDGTFRP
                     RLEYGTGSEPRALAVGDVSGDGKPDVVTANHSENTVSILKNIAQPPTTAIHAITLTAE
                     ATASRVRIEWYAPGDDILEASVYRRTDDSDWALQGHPEPDMQRRILYEDDTVVSGVRY
                     GYRLVLLDVTGQESAVETWVSVPENAPAVIRLEAPRPNPFVNRVELTYGLPHDGRVRL
                     ALYDVRGRRVALIVDRVEAAGWRSIGWNGRNGSGRELPSGAYFLKLEYGDQTQVRKVL
                     IAR"
     gene            1643..3871
                     /locus_tag="VFR25_10180"
     CDS             1643..3871
                     /locus_tag="VFR25_10180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9941453.1"
                     /translation="MGDSHHFSENLPSATTSGRPQRPVRVMHVVDTLLLAGMEYGVIK
                     ISNGMPAEIEPSICCLRFQTDVTKAVLDPRVRVVALNRPTRHNYSLIGTLANTFRDEG
                     IDIVHSHNWQTYLYAVLGARLAGVPVVIHGEHGHDDLAPSARRLWIKRRLAPLVTRFI
                     AVARGLERELEEVWGIAPERIQFIPNGVDLTQFDPKSDGTPVRREFGLGPEHEVVTTI
                     GGLRLVKDHATLLRAFARVHEARPNARLLVVGGASRPELYADLVTLADSLGVTDQVVW
                     TGVRKDVARLLAATDVYVNSSTFEGMSNTILEAMAMGVAVVATNVTGNADLVRDGGTG
                     VLTPPANPEAMAAAIRTLFEDPERRHELGDTARRFVETDHRMDAMIRTYANLYLETHD
                     RSWERLRFSPRQNAKALLSRSLTALGLPIFRRRVTPPGLGILTYHRVLPLHESRMYAH
                     QGMVMPRDEFESQIGHLDRHYHVLPFPEAVERLKAHTLPPKALTITFDDGYRDNYEHA
                     WPLLRKYGLPATFFLVTDSLDRKERLWWDEVVEAVLRMGSNGISEDWLEPRVRAALGT
                     DYKIDPRRAAGRIVRLLNEAPRAERVRAIQMLRECTGCTPEDQGRLMMSWEEAREMLG
                     SGMTFGSHTRTHAFLDELSDDEVASEVRWSTDRIRAMLDPTSRWLAYPRRPAMSRGAA
                     ILAQAGIEAAVSTDPGLNPPGSDLYALRRIDAGYFSVRAGFDPAILDAELSGLFQRLR
                     GA"
     gene            3889..4431
                     /locus_tag="VFR25_10185"
     CDS             3889..4431
                     /locus_tag="VFR25_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9941454.1"
                     /translation="MNSETRRIVVTGAPGTGKTAILSAITGIDIVPEPARIVLAEQRA
                     LDETGTPERDPRRFVELLLGRSIENHRNAKAGKPVLFDRGVPDCIAYALLLGADPEPC
                     IQATSRYRYEATVLVARPWAEIYTTDDERKMTFDATLEFQRLIEEAYLGAGYMLVEIP
                     RGKLEERAAFVRHFLEQARV"
     gene            4470..4546
                     /locus_tag="VFR25_10190"
     tRNA            4470..4546
                     /locus_tag="VFR25_10190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4504..4506,aa:Arg,seq:ccg)
     gene            4641..4889
                     /locus_tag="VFR25_10195"
     CDS             4641..4889
                     /locus_tag="VFR25_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941455.1"
                     /translation="MTDPAPAKPPFRWTERRTARFVSMVISGAVVLADIYLLTVVLSV
                     DSTAMGGWKPAIMAGIGAIFLFAMYRFRRLLRLFLHEE"
     gene            5009..6100
                     /locus_tag="VFR25_10200"
     CDS             5009..6100
                     /locus_tag="VFR25_10200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET9941456.1"
                     /translation="MDDLTGNELFFATLQEITSLINGGRAQETIFESVLSCALQMLHA
                     QAVFLITVDGGRIRKFARRVSADGQLHDVERYELPSHAGISRWVLLEGQPVHVPDVGA
                     DPRYHPSVDSLPGLRTSAVLAAPLKVRDAVLGVLVAVNRVQENVPFDLRHLRILSLLA
                     NQTAIAIENGKLFRRAEQLAVTDDLTQVYNYRFLKMALRREVKRAARFSQRFSVLMID
                     VDNLKRYNDRNGHLRGSEVLRMIAQVLVREARSIDLVAKYGGDEFVIILPQTEREGAR
                     VLADRIKMSVETTAFPLVPPGIITVSLGLATYPDNGLTAGELLESADIALYAAKESGK
                     NRVSTAGVESSGSLRRRRDPHGHGPAESL"
     gene            6132..6797
                     /locus_tag="VFR25_10205"
     CDS             6132..6797
                     /locus_tag="VFR25_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941457.1"
                     /translation="MPATLHDPALVLGSLAAVLVLVYLTRFVARALHTSPEGQRKALH
                     ALIGAWTLFVTPAFHHLAWALVPPVVFGVVNASAWGKTLMPDLARSAHESRGLWTFPL
                     AVVLLYIVFWDAPPRSAILAGLGVLALADPVASLVGARWGQRKLVWGGGRTLEGTLAF
                     LVVASIVVGTIAAVTGAQGAFPWRMGVGCGAVGAIVEAWTPAGWDNLTIPLAVAGTYH
                     LLA"
     gene            6805..7551
                     /locus_tag="VFR25_10210"
     CDS             6805..7551
                     /locus_tag="VFR25_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HET9941458.1"
                     /translation="MIAPTPTTIALGLACAGAGLVATTGQGTWRGALAGLAIAFVAIL
                     GLGPGALLPLGIFVLGSGALTRIGRARKEAAGAAEAHRGRRGIANVAAKLGLPAFLGA
                     VGLLVDPASGAMELVRLMYAAALAAAFADTAATEVGPIARGPVWALHGLRPKRVEHGT
                     PGGVSQAGALTSVAAAALVGWAASRSDLILPSQWPLTAAAGVVATWVESALAGTALGR
                     SLGHHGRNACLSAVAVSLGGVAWIAMGGHR"
     gene            7524..8678
                     /gene="wecB"
                     /locus_tag="VFR25_10215"
     CDS             7524..8678
                     /gene="wecB"
                     /locus_tag="VFR25_10215"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET9941459.1"
                     /translation="MDRNGGASVIEIVCVVGARPNFMKMAPILDALRAYPDIKPFLVH
                     TGQHYDEEMSRVFFQELGLPRPDRDLEVGSDSHGRQTGKIMMAFDSLLDGRSTRLVVV
                     VGDVNSTVACGLVAVKRGIALAHVEAGLRSRDRTMPEEINRLVTDAISDLLFATSPDA
                     IENLRAEGVPDSKIHLVGNPMIDSIHRHREAARARNVPARFGLAQRRYGLVTLHRPSN
                     VDDPGTLAGILESLQALAKRAPILFPVHPRTRERIRSLGFQPGPGLALNEPLGYLDFL
                     GLMDQATFVLTDSGGIQEETAALGVPCLTLRANTERPITLTEGTNRLLGTDPRAIVPA
                     ATALLDGDAPAPKCPALWDGHAGQRIAKVLADHVNQGASAENRKGVQVDT"
     gene            8769..9734
                     /locus_tag="VFR25_10220"
     CDS             8769..9734
                     /locus_tag="VFR25_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9941460.1"
                     /translation="MASALLAVLFLVSLPAVVLAQRSDTPPPGDRNYKIGIGDLLKIE
                     VVGRPDLSGQMEVGIDGRVTLPVVGAVKAADRTTAELSNDLSRRISLVQRVAPEVIVS
                     VLEYRSRKVFVLGSVLIAGTYSIGATAMSVWDAIAEAGGPTEDANLSAVEVIPGDISG
                     GSQVRTVDVAAAIRDGKLDDLEPLRPGDTVRVPRGGGSAAGIGNIVYIFGAVGAQGPH
                     PFDPGMGLVQALIRSTPSADANFSKIEIVRNAGPRIMRMEIDLNEYLGHASLTGNPEL
                     QAGDTIFVPRSGRGFAGVGWLGLLGVLGTVLGLVVAVDAIANNNH"
     gene            9745..11421
                     /locus_tag="VFR25_10225"
     CDS             9745..11421
                     /locus_tag="VFR25_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941461.1"
                     /translation="MDPHMPHPYPAPYPQAPPPEKQPVFDIRDVLWRVTRYRWLLVVP
                     IVISMSGAAIYYTLAPEIFESHVLVAIDDQAQVSGALQGLVQPDRDRDTPRERVTLVD
                     SKVRSRRFLQELSLRSGFNRDPQVVNDAERANRRWRGVSSEEFAIRFASARLSKRIRV
                     TPVEGTYVRISAMGPSPGEARRIASLIADILIEESKRTNLERVQARGEFSADQITVYE
                     ERLRKSEDAYQRYQESLIRNVLASNPVNDRNIEDARGLINMAESEMAQVKQRLESEQR
                     DFAGASNGASVPSLQGGKAIELSNRLVELESSASAAALSDKEKSDRNIAGIQMSIAST
                     RQMLLAELELEASQLQGNLSDEAQSAATGIALDRAIIRSLTLKRDRLRGLISDYARTV
                     QSTPREDMELARLKGEVDTNRDLLNALRREQTSSRISEALETSGLGFRLSVLEPPQLP
                     YRASEPDPFKIFAAAALLGPLGAAGIVLLGEKVRLVVRSVEQAEGELGARVIATFPRI
                     EGWSRPGTYFQNNWPVMVIFAVVLVTGTFFALNATLFAPHPVVKKTQTKK"
     gene            11433..12224
                     /locus_tag="VFR25_10230"
     CDS             11433..12224
                     /locus_tag="VFR25_10230"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET9941462.1"
                     /translation="MKRPQRTLFDSFNLDAPHVTEARRLLQTLARRERDESNTPKVYM
                     VTSAGRGEGKSTICGLVSMVAARIFRRKTLVIDADLRRPSMHSLLGLNQRPGLAELME
                     GSASVEEATRSTLIGTLSAIPSGRPTKSVGDTYDDFAFRELLDQFRPQYDLIFIDCPP
                     VVPVMEPLLIAEHVDALLLVAMAGATQVTMIRRMRQILAPLEPKIAGAILNNATDGLP
                     YYYDYRYYGYESPKPKRVREIRTPAKPSSQDDAAARQVARERGGA"
     gene            12224..13582
                     /locus_tag="VFR25_10235"
     CDS             12224..13582
                     /locus_tag="VFR25_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9941463.1"
                     /translation="MAGRNGSRSAARTKRSGGSPLPGFEAKVQNGTARIGIIGLGYVG
                     LPLAVEFAKKGFRVTGFEINKKRVGVLNSGRSYIQDVPTAEVRELVKSGKLHATLDFN
                     ELKAMDVIDIAVPTPLRKTKDPDVSFIVAAVSEIAPRLKRGQLVVLESTTYPGTTDEL
                     VRPMLEEGGLRAGRDFFLAFSPERVDPGNPTYQTKNIPKVVGGTSADCTRAAALLYGT
                     VIDRIVPVQSTQVAETVKLLENTFRSVNIGLVNEVALMCDKMKIDVWEVIDAAKTKPF
                     GFMPFYPGPGLGGHCIPIDPFYLSWKARQSGFEARFIDLAGQVNANMPHHVARRVGEA
                     LNTRRKSVRGARVLVLGIAYKADIDDVRESPSLDIIETLANDGAKVEYSDPFCPSLEH
                     NGRRFTSRPLTPATLRAADCVVIATAHKMFDYGLIAKHAQTVVDCRNVLKGRRTRGVF
                     RI"
     gene            13590..14540
                     /locus_tag="VFR25_10240"
     CDS             13590..14540
                     /locus_tag="VFR25_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9941464.1"
                     /translation="MAHYLVTGGAGFIGSHLAERLLKDGHRVRVLDNLSTGRVSNLEA
                     VRAAGPADRLEWIEGDIRSLETCRRATAGVDYVLHQAALASVPRSIENPIDSTAVNVG
                     GALNVFHASRDNGVKRVVAASSSSVYGDAATLPKHEEQPTAPLSPYAASKLAGEIYAR
                     VYAVTMGLSTVSLRYFNVFGPRQDPNSQYAAVVPRFVTALRNGQRPVVYGDGLQSRDF
                     TFIDNVVAANLAACTEGPGRGEAMNIACGDRYTLLQLLDVIGRLLGVTPDPEFQPARP
                     GDVKHSQASIELAQRLIGYRPAVGFEEGLARTVESFQPAG"
     gene            14540..15592
                     /locus_tag="VFR25_10245"
     CDS             14540..15592
                     /locus_tag="VFR25_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HET9941465.1"
                     /translation="MTAPTTAVASVAIAEAPLDGTGWADYAAAHPAATFFHQPGWLEV
                     VRRTFGYRHASILARRDGRVVGFLPLFLVPTLPWGRSLISTPQAVYGGPIADDDEARS
                     ALVDHAARLAEARGASFLELRNETPIPGLAGKDLYVTFRRPIHPDAEQNMAAIPRNQR
                     RSIRVAQKHGLTARVGHGDLLDGFYRVYSESVRNLGTPVFPRALFANVLERFGEDAFI
                     LGIYKGDQMLSAVLTFRYRDQILPYYGGATREGIQYATNDFMYWSLLLNGMERGLGVF
                     DFGRSKVDSGSYHFKRHWGFEPTPLAYQYHLAPGKALPDLSPRNPKFSMAIRVWRRLP
                     LKLTQWVGPHLVRYFP"
     gene            15589..16833
                     /locus_tag="VFR25_10250"
     CDS             15589..16833
                     /locus_tag="VFR25_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET9941466.1"
                     /translation="MNILYLCHRIPFPPDKGDKIRSYHQIESLGARHRVHVATFVDSP
                     EDERHVPELKERCASLVVDRRTTATNLTRAAGALLTGDPFSVACFRSSRIARGVDRAL
                     HEEGIDLALVFSSGMAQYVERAQGIARVIDFVDVDSEKWRAYAARERGPLRLIYAREA
                     DLLLRHDARIAHAFDRSLFVSETEAALFRERTGAGNVAAIPMGVDLDYFAPAPEPATE
                     PVLVFVGMMSYMPNVDAMRFFVGDVLPLVRREVPNVRLHIVGREPAAGVRDLADPPRV
                     VVTGAVPDVRPYLREAAVAIAPFRISRGMQSKVLEAMASGLPVVGTRLAFQGIPAGSE
                     DGVESAEGADALAAALIRLLRNPTTRRDRGGRARRYVERHHRWADHGEALDHLLEDVV
                     ARRRSERTPLGAGARTAGGRSV"
     gene            16917..18800
                     /gene="asnB"
                     /locus_tag="VFR25_10255"
     CDS             16917..18800
                     /gene="asnB"
                     /locus_tag="VFR25_10255"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9941467.1"
                     /translation="MCGIAGAVETADPSRLTDSVLQRMASLIAHRGPDNQGAWLAPPA
                     GLCHRRLSIIDLSEAGNQPMSLEGTGLTMVFNGEIYNFPELRSELEGKGVVFRSRSDT
                     EVLLRAYEAWDVGCLDHINGMFAFAIWDAPRRRLFAARDRLGKKPFFYHARPGRFVFG
                     SEMKSILGYGGIDRDIDPHALDEFLSTGCIGAPRTILRSIRKLPPAHYLLLEGDRITI
                     RPYWDVTFRVTEPERTEGERIERLEHLFREAVRRRMISDVPLGAFLSGGIDSSAVVGM
                     MAQLSDRPVKTYTIGFEEKGYSEIEDARVIARHFGTEHHEFHVRPEAIGILPDLVWHF
                     DEPFGDSSAIPTYYVSKIAAEHVKVALAGDGGDELFAGYTRYQDSLRVPPYHLIPASV
                     RRRVLAPLADRMPLAAPGRNRLYAAAHHAPLTPGYGLGLYPYIKEQLLSPEWRTQTAL
                     REPNGHTAVSEPRLRSMDLLSRLQYIDTKVYLPEDILTKVDRMSMAHSLEVRAPLLDY
                     TLIEYVASIPPALKLRDSVSKYLFRKMASRYLPASVFEKPKQGFGVPTGAWFQQELRV
                     SARERLLDRRTLDRGYFRRRTVERVLDLHEAGQRDYGTWIWCLLVLEEWHRMFLDADT
                     RRV"
     gene            18817..20703
                     /locus_tag="VFR25_10260"
     CDS             18817..20703
                     /locus_tag="VFR25_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HET9941468.1"
                     /translation="MCGICGIVDFRSPPSLELLGRMTNALRHRGPDGNGCYHDGPAAL
                     GHARLAIIDLVTGDQPMWTEDRSLAVVLNGEIYNFRSLRKALEARGHTFHTQSDTEVI
                     LKAYEEYGEDCARHLRGMFTIALWDRRRQCMFLARDRVGIKPLYYAWDGHRFLFGSEI
                     KALLESPDLDRTVDPLALDDYLTYLYVPAPKTIFTHVRKLPPAHTMRVDRDGLHVREY
                     WDISFEPRRDWTEQGAAEAIAEKLRDSVECHLISDVPLGAFLSGGVDSSAVVATMAGL
                     IDHPVQTASIGFKEAAWDELPYARQVAQRFGTQSHERIVDPHAARILDDLAWHYDEPF
                     ADSSMIPTYYVSAVARERVTVALSGDGGDENFAGYRRYKFDAFENRIRAVLPGAIRRP
                     VFGTLGRIYPKADWAPRIFRGKTLLSNLARDAERAYGHSVSCFTPAMRQSLYRPEAAA
                     MVRGYDSHSVLQTWFDRARDWDPLSRVQYVDIHTYLVDDILTKVDRASMAHSLEVRVP
                     LLDHEFMELAASIPAGMKLRNGEGKYIFKKALGTTLPDDVMNRPKMGFSIPIHEWFRG
                     ELKPVFESAVFAPDAFVAERLRTEEIRRLWTLHQRGARDFSPYLWGILMLEHWGRRFL
                     RRSG"
     gene            20700..21905
                     /locus_tag="VFR25_10265"
     CDS             20700..21905
                     /locus_tag="VFR25_10265"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9941469.1"
                     /translation="MKVLVFTSLFPNNVWPNQAVFVRERILHLARIPGVELKVVAPVP
                     YYPPLPGWRAAFRRVASREERAGIEILHPRYTMLPKVGSRWHGAFLHRALRTRMERLR
                     REFRFDVIDAHYLYPDAYAAVRLGRELGVPVVSSARGSDVNLIGQMPDLAPLVREVLV
                     GSKRLIAVSAALAHAMREMGAPEVSVIPNGVDATRFHPVDRGSARRDLELGEGPVILS
                     VGNLVPNKGMDRLLRALALASVNPGSTASRATLVIAGRGPEEERLRRLAEWHGLHHRV
                     RFTGVLAHESLGPWYAAADLFVLATEREGWPNAILESLACGTPAVATRVGGVPELLRS
                     ERVGIMVEPSDEAFARAIEEGLDRAWDRAEIARFAAGHTWDATARSAHAVLESALDRA
                     PAREARVAS"
     gene            21895..23223
                     /locus_tag="VFR25_10270"
     CDS             21895..23223
                     /locus_tag="VFR25_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET9941470.1"
                     /translation="MHPEAARSAEAIGNALPDGLKVLHVLDHSLPLHAGYSFRTASIL
                     HAQRKQGLEPVAVTSPKHEESWKKETPPVETIEDVRYYRTGLVPPSPIPLAYEMKLMG
                     ALERRIREVARIERPDLIHAHSPILCAVPALRVGRALGLPVVYEVRAFWEDAAVDHGT
                     DRAGSIRYRTVRGVETWACKSADHVVVICGGMKDELLGRGIPSDKIDISWNAIDPGSF
                     QPGEPDRAFAREHGLEGKRVLGFLGSFYGYEGLELLVSAMGALRGRREDLKLLLVGGG
                     PREQALRDQIRREGLEQVVVMPGRIAKERIPGLYGLVDILVYPRYSTRLTELVTPLKP
                     LEAMAMGKPLVASDIGGHRELIQDRRTGLLFSPGSVPSLVSAVETILDDDALRADLVR
                     GGREWVETNRAWSITTSVYSKVYTQARLRAERRGRDSWKRNSQGEAAEAR"
     gene            23244..23936
                     /locus_tag="VFR25_10275"
     CDS             23244..23936
                     /locus_tag="VFR25_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941471.1"
                     /translation="MHRAPRTIALVLRASLVAFLVLIGLGSGMASAQETQGEQGDAWL
                     NRNYFGRHTDGFTQELLANAERNHLAQENFWQKYRSGELDRALGDLKYVLLVFPNHPR
                     ALHLMTLICRTMKDSTTPIVYFEKAVRTFPNEPYTFAQYGAYLISTGDTEQGIARLKD
                     ALRLDPNLTYALGLLSEAQKKPKAGTTPGAGTTGSTAAPGSKAPAAATPNANSPTTSA
                     APSTSTPGTTVR"
     gene            23956..25854
                     /locus_tag="VFR25_10280"
     CDS             23956..25854
                     /locus_tag="VFR25_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HET9941472.1"
                     /translation="MSGLWGFVLADPRGRADISDLAVMGAGRHPGATHPLHAVAAGPA
                     ALAAQEFPNRAAAVARRQVGDSILLLALHGSILELDGSGSTHDGNAPKSANLLADRLL
                     EGYLGAGQDLWQRIRGDFVIAIWDGRDATFRVACDRFRTHQFFYSVDMRGLTFGSRIA
                     PILARPGTGSPGIDSDSVLEVLLASVIPTPKTIYEGVAKLPPGTELIYRSGTMNLRPY
                     WDMAFGTSERSSERELARDLRESLEDAMRVRLAMDRDASYGAFLSGGIDSTTVLGLAT
                     KIAGRPVKSFSIGFGEPRFNEMEYARIAARAFGSEHHEYFVQPEDVVRAIPTLLDAYD
                     EPFGNASAIPTYYCATVAREHGIDVLYAGDGGDELFAGNERYSVQRLFDYYRKVPSWL
                     REPVLRPLVETLAATKVPLFVKGAKYVRRARTPYVDRISSYDLDHVLPLDEIVAPAQL
                     ERITRQHSHKALLSHYYRSAAAADDLDRHLYLDLKLTISDNDLIKVTRATEAAGVAVR
                     FPFLDSRVAEVAGAVPARIRMRGTELRSFFKRAYSDFLPPETIAKTKHGFGLPIPVWL
                     RTDPTLNGMMRDLVLGPSNRVAGYFRRSALETLVERHSTDPTTYYGVFLWNLMVLELW
                     LRRHASTT"
     gene            25851..27020
                     /locus_tag="VFR25_10285"
     CDS             25851..27020
                     /locus_tag="VFR25_10285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9941473.1"
                     /translation="MRPGPGAGADTQPRSGVRVEVLRTAEELDRLVPEWEALLARSAS
                     DTIFLSPDWLRSWWAAYGSGRELIALRVVSDGSLVGLALLYRGLGQLVAGMRHRTLVL
                     LGDGSADSDYLDWISARGQEGTVVAGILDGLRTSVPGWDLLLLNEIPETSPHLARLQA
                     EAATRGWLWHDWRVPCARVSLPASWDAYVKSLKPRMRTKVRSVLRVMDEEFHARYERL
                     TDLSELEARLQSLYDLHQRRWEADGKSGVFQTATKRDFYALLSRRLLESDRLRFYSLR
                     IGTDYVAHQYCFEYGNRMYLLQEGLDPAWFEHGAGNALRAHVFQDCIARGIAVYDFLG
                     GVTAHKLSWGAETVWSARVTTGPPSWKNRILFGVRGAKAALKKLLRPERVTEDAG"
     gene            27017..28078
                     /locus_tag="VFR25_10290"
     CDS             27017..28078
                     /locus_tag="VFR25_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9941474.1"
                     /translation="MTRTVVRRWAARVAFGLGLTQTFTRAGDRWRVLRGNGGVSVGRR
                     RRGAFLVLLYHRVNRASAHFAIERTETEAFRAQMEHLARHFHVMSLDDLVKRVTAGES
                     LPSRAVAITFDDGYEDNYTDAYPILRKLGLPATVFLATASIGTGKSLWFDRVLDGFRT
                     ARAHSIKLPGGESAEGLVDPMIRETMAHRALYALMRLSNAERETAIDTLLRNLGPEGP
                     PNLPAMMTWDQVRLMAANGIDFGAHTMTHPILSRLPLDEAEAEIVGSKRRIEEEIGTP
                     VRLFAYPVGRRSDYSPEVIDLVARSGFHAALTTTPTANLAGDDPFLLKRIKPLGDDLP
                     SFALGLSAQYVTEWRQAVP"
     gene            28075..29544
                     /locus_tag="VFR25_10295"
     CDS             28075..29544
                     /locus_tag="VFR25_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET9941475.1"
                     /translation="MSQAAPAPESIRTLAIRSSFVNLAASFGLKGLTLAQAVIYARVF
                     VPGELGQVATVLLVISFMTVITRMGFEESIVRENDHPERVMQTAFFLALVLGAILSAG
                     LYVAAPLVASAFHRAELTQYLRFMAFMVFGSALGLPNALWAQRFRFGLAKVASFADLI
                     VGTVVTLVLHFHAGLGIWSLLWGRIAGFAANYLTVWIIAAYRPWPVFDRAQARVLLSF
                     GWPIVVGSLANYLMHQGDAVIVRYFHGDATLAYYQLAYAFPYYLMEGTDVLLGALLPT
                     FSRLRDSRERLVSAFVQSNKYITLAIVPCGIALAALAGPLVRVVFGAKWLPAVGPMTV
                     LAIGFTVQVMWGYGWGALVLASGRTRYLMYVKLWIVVYLLTAGTLLIRAFGAMGGALY
                     VLTQAILTVGVVRGWILQHELGSRSFIKDSWKPVVASLVPGALVWWLASPHIGSVVGL
                     IFTGIAYGAVYIGLMSVFDRELVPELRHMARLVFGPRAA"
     gene            29555..30865
                     /locus_tag="VFR25_10300"
     CDS             29555..30865
                     /locus_tag="VFR25_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HET9941476.1"
                     /translation="MRDYLIMAFVFSILPYAFMRPYVGALMWVWISIMNPHRLTWGPA
                     YGFPFAMLTAVTTMAGVFISNDRGRFPAARETTIMILMAAWFTLTTTVALMPEAAWAK
                     WDRVIKILIMTIVPLFLLQDKNRLRLLLLVMVASIAFFSVKGGLFSLLTGGRYRVYGP
                     SQSFIADNNALAVAELMVLPLMLYLAKTEPRKWVKRGLYLSVPLTLVSVVFSYSRGAL
                     LGLLAMVAVLIVTGRRWGLAAAFIVLAGVGFVFIPNQWFERMSTIGTYKQDESAMARF
                     QSWGFAWNVATDRPLTGGGFRVFSTDQFLRYGPDPFEAYDSHSIYFEVLGEHGFVGLI
                     LFLSLLVSSFLSLNWVERQIRGRPSIAWAKDYAVMLRLSLVSYAVGGAFLGLAYFDLY
                     FDIVAAVVLLKILVQRELAQEAAVSRAYRLSAPAFAPPRATSAT"
     gene            30990..>31587
                     /locus_tag="VFR25_10305"
     CDS             30990..>31587
                     /locus_tag="VFR25_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9941477.1"
                     /translation="MIHGKRLVVVLPAYNAARTLQKTYEELPHEIIDDVILVDDASGD
                     ETAEMARRLGIRTFIHPKNRGYGGNQKTCYREALQCGADIVIMVHPDYQYTPALVTAM
                     SSLIGMGVYDSVLGSRMLGTSALKGGMPRYKYVANRILTFFQNVMVGRKLSEYHTGYR
                     AFRREVLQTLPLEENSDDFVFDNEMLTQIIAFGYSIGEV"
BASE COUNT         5036 a        10785 c        10405 g         5361 t
ORIGIN      
        1 tcttgcaacc gcggacttca gtgcgagcag cgcgtctgtg ctcttgggca atggagacgg
       61 cacgttccag ccgaaggtgg actacgggac gtggaccaac cccaccgcca ttgccatcgg
      121 ggacgtgagc gcggatggga agccggatct cgtgacgacg gggtccagtt tgggcttcaa
      181 catggtgtcg gtgctcctca acaacgggga cggcacgttt cagacgaagg tggattacga
      241 agtggggact ttgccctggg cggtcgcgat cggagatgtg agcggggaca gcaagtccga
      301 catcgtgact gcgaattact tcggcggcac cctgtcggtg ctcctgggca acggcgacgg
      361 aacgttccag gcgagcgtgg actacgggac ggcggacagc ccgatctccg tcgcgatccg
      421 ggacgtgagc ggggacggga agccggatct catagcggcg gccaacggcg gcgtttcggt
      481 gcacttgggc aacggggacg gcacgttcca gccgaagaca gactatcccg gggtttccgc
      541 ctattcagtg gcgatcgggg acgtgagtct ggacggggag ctggatattg tgactgcgaa
      601 tggaacgaac accgtatcgg ccttcctcgg caatggggat ggcaccttcc ggcctagact
      661 ggaatacgga acgggctcgg agccgcgggc cctggcggtc ggggacgtaa gcggggacgg
      721 aaagcccgat gtcgtgacgg cgaatcacag cgagaacacg gtatccatcc tcaagaacat
      781 cgcgcaaccg ccgacgaccg ccatccacgc gataacgctg accgctgagg ccaccgcaag
      841 ccgagtccgc atcgagtggt acgctccggg tgacgacatc ctcgaagcga gtgtctatcg
      901 acgcaccgac gacagcgatt gggcgctcca ggggcatcct gagcccgaca tgcaacgaag
      961 gatcctctac gaggacgaca ccgtcgtgtc cggcgttcgc tacggctatc gactcgtcct
     1021 cctggacgtg accggacaag agagtgccgt cgagacgtgg gtgtcggtgc cggagaacgc
     1081 tcccgccgtg attcgactgg aggcgccgcg acccaacccg tttgtgaacc gagtcgaact
     1141 cacgtacgga ctcccgcacg acggacgcgt tcggctggcc ctgtacgacg tgagggggcg
     1201 gcgcgtggcg ctgatcgtgg atcgggtcga ggcggcagga tggaggtcga tcggctggaa
     1261 cggacggaat ggaagcggtc gggagctccc ttcgggggcg tacttcctga agctcgagta
     1321 cggggaccag acgcaggtgc gcaaggtttt gatcgcgcga tagcctgagg tgggctcgat
     1381 agctagtccc tacgaagcga actacaccgc gagggtggcc gtaacacgat catcccgcta
     1441 gaatgcgcgc cgaggggggg aagggccccg cccgcgggcc cagggtctgc gcatccagaa
     1501 ttccgacttc gcgagttcat ggacgtttcg gtttggcaag gattgccccc cagcctcaac
     1561 acaccgcgga ccgccccggc gcttccactt cccatgggtg atgcccgaac taggtatttc
     1621 tacctagcgg ggaggcgcag cgatgggtga ctcacaccac ttctccgaaa acctgccgtc
     1681 cgccacaacg tcggggaggc cccagcgtcc ggtccgggtc atgcacgtcg tggacacgct
     1741 cctgctcgcc ggaatggagt acggcgtgat caagatctcg aacggtatgc cggcggagat
     1801 cgaaccgagc atctgctgct tgcgattcca gacggacgtc acgaaagcgg tgctcgatcc
     1861 gcgggtccgg gtcgtcgccc tcaaccgccc gacgcggcac aactacagtc tcatcggcac
     1921 actcgccaat accttccggg acgaaggaat cgacatcgtc cattcgcaca actggcagac
     1981 ctatctctac gccgtcctcg gtgcgcgcct cgcaggggtg cccgtggtga tccacggcga
     2041 acacggccat gacgatctcg cgccgtccgc gcgccggctc tggatcaagc gccgcctggc
     2101 tccgctcgtc acgcggttca tcgcggtggc acggggcctc gagcgggagc tcgaagaggt
     2161 atgggggatc gctccggagc gaatccagtt catcccaaac ggcgtcgatc tgacccaatt
     2221 cgaccccaag agcgatggga cgcctgtgcg ccgtgagttt gggctcggcc cggagcacga
     2281 ggtggtgacg acgatcggcg gcctccggct cgtcaaggac cacgcaaccc tgcttcgcgc
     2341 attcgcgcgc gttcacgaag cccgcccgaa cgcgaggctc ctggtcgtgg ggggcgcgtc
     2401 ccggccggag ctttacgccg atctcgtgac gctcgcggac tccctcggcg tcacggacca
     2461 ggtggtctgg accggagttc ggaaggacgt cgcgcgactc ctcgcggcca cggacgtcta
     2521 cgtgaacagc tccacgttcg aaggcatgtc gaatacgatc ctcgaggcca tggcgatggg
     2581 cgtggcggtg gtcgcgacga acgtcaccgg aaacgccgac ctcgtgcgcg acggagggac
     2641 tggcgtgctc actccgccgg cgaaccccga ggcgatggcc gcggcgatcc gcacgttgtt
     2701 cgaagatccg gaacggcgcc acgagctggg ggacacggcc cggcgctttg tcgagacgga
     2761 ccatcggatg gacgcgatga tccgtaccta cgcgaatctc tacctcgaga cccatgaccg
     2821 cagctgggag cgcctccgat tttcgccccg gcagaacgcc aaggcgctcc tctcccgctc
     2881 gctcacggcg ctcgggctgc ccatattccg gcgccgcgtg acgccgccgg gcctcggcat
     2941 cctgacctac caccgcgtgc tcccgctcca cgagtcgcgc atgtacgcgc accaggggat
     3001 ggtcatgccg cgggacgagt tcgagtcgca gatcggacat ctggatcgcc actaccatgt
     3061 gctgccgttc cccgaggcgg tggagcgact caaggcgcat acccttccac ccaaggcgct
     3121 cacgatcacc ttcgacgacg ggtatcgcga caactacgaa cacgcctggc ctcttctcag
     3181 gaagtatggc cttcccgcga cgttcttcct cgtgacggat tccttggacc gtaaggagcg
     3241 gctctggtgg gacgaggtgg tcgaggcggt gctccggatg gggagcaacg ggatctccga
     3301 ggactggctc gagccccgag tgagggcggc cctcggcact gactacaaga ttgatccgcg
     3361 acgcgccgcc gggcgcatcg tccggctctt gaacgaggcc cctcgcgccg agcgtgtgcg
     3421 cgccatccag atgcttcgtg agtgcacggg ctgcacgccc gaggaccagg ggcgcctcat
     3481 gatgtcctgg gaggaagcgc gcgagatgct gggatccgga atgaccttcg gatcgcacac
     3541 ccgcacccac gccttcctcg acgagctctc ggacgacgag gtcgcgagcg aggtgcgctg
     3601 gagcacggat cgcatccggg caatgctcga tcccacgtcc cgctggctcg cgtatccgcg
     3661 acgaccagcc atgtcgcgcg gcgccgccat cctcgcacag gccgggatcg aggcggccgt
     3721 gagcacggat cccggactca accctccggg gtcggacctg tacgcgttgc ggcggatcga
     3781 cgccggctac ttcagtgtgc gggccgggtt cgatccggcg atcctggacg ccgagctgag
     3841 cggtctcttc cagcggctcc gcggcgcctg atcaccggcc atggggtcat gaattcggaa
     3901 acccggcgga tcgtcgtcac cggcgcgccc ggtacgggga agacggcgat cctgagcgcg
     3961 atcaccggca tcgacatcgt gcccgagccc gcgcgcatcg ttctggccga gcaacgggct
     4021 ctcgacgaaa ccggcacgcc cgagcgcgat ccgcgacgtt tcgtggagct cctcctcggg
     4081 cgctcgatcg agaaccatcg gaacgcgaag gcggggaagc ccgtcctctt cgaccgcggc
     4141 gtccccgact gcatcgcgta cgcgttgttg ctcggcgccg atcccgagcc gtgcattcag
     4201 gccacctccc ggtaccggta tgaagccacc gtcctggtcg cccgtccctg ggcggagatc
     4261 tacacgacgg acgacgagcg gaagatgacg ttcgatgcga cgctcgaatt ccagcgtctg
     4321 atcgaggagg cgtacctcgg ggccggctac atgctcgtcg agatcccacg cggcaagctc
     4381 gaggagcgtg ccgcgttcgt ccggcacttc ctcgagcagg cgagggtcta ggcaggggtg
     4441 cggcgaaccg ctagaatgcc aacccacttg cgcccgtagc tcagctggat agagcgtctg
     4501 cctccggagc agaaggccag gggttcgaat cccttcgggc gcaccaccca actactggct
     4561 gaagacagat cgccccacgg agcctggctc cgtgggggtt tcatttcctc gcacaaccgc
     4621 gcttcgcgta ccatcccggc atgacggacc ccgccccggc caagccgccg ttccgctgga
     4681 ccgagcgccg cacggcgcgc ttcgtctcca tggtcatcag cggagcggtc gtgctcgcgg
     4741 acatctatct gctgacggtg gtcctctccg tcgactccac ggccatgggc gggtggaaac
     4801 ccgccatcat ggccggaatc ggagcgatct tcctgttcgc catgtaccgc ttccgacgcc
     4861 tcctccggct attcctccac gaggagtaac cgcagcccgc ctcgcgtctt aagacctcac
     4921 cacccagcgc gcctcgagtc cggggcacgg cgattgcttt gatccagcca tgatggactc
     4981 tcaaactcct aaaaccttgc agcctgcaat ggatgacctg acggggaacg aactgttttt
     5041 tgccaccctc caggaaatta ccagcctgat caacggcggg cgggcgcagg aaaccatctt
     5101 cgagtcggtc ttgagctgcg cactccagat gctgcacgcg caggcggtct tcctcattac
     5161 cgtcgacggc ggacgcatcc ggaagttcgc gcggcgcgtg tcggccgacg gtcagctcca
     5221 cgacgtcgaa cgctacgagc tgccgagcca cgcggggatc tcccgctggg tgctcctcga
     5281 agggcagccg gtgcacgtgc ccgacgtcgg cgccgatccg cgctaccacc cgagcgtgga
     5341 ctcgctcccc ggccttcgca cgtccgccgt tctcgcggcg ccgctcaagg tgagggacgc
     5401 ggtgctcggc gtgctcgtgg ccgtgaaccg ggtccaggag aacgtgccgt tcgatctccg
     5461 gcacctgcgc atcctgagtc tcctcgccaa ccagaccgcg atcgcgatcg agaacgggaa
     5521 gctcttccgc cgcgccgagc agctcgcggt gaccgacgac ctgacgcagg tctacaacta
     5581 ccgcttcctc aagatggccc tccgccgcga ggtgaagcgc gcggcgcggt tcagccagcg
     5641 gttctcggtg ctcatgatcg acgtcgacaa tctgaagcga tacaacgacc ggaacggcca
     5701 tctgcgcggg agcgaggtgc tccgcatgat cgcgcaggtg ctcgtgcgcg aggcccgctc
     5761 catcgacctc gtggccaagt atggcggaga cgagttcgtg atcatcctgc cgcagaccga
     5821 gcgcgagggc gcgcgcgtgc tcgcggaccg gatcaagatg tcggtggaga ccacggcgtt
     5881 tcctctcgtt ccgcccggta tcatcaccgt gagcctcggg ctcgcgacct accccgacaa
     5941 cgggctcacc gcgggggagc tcctcgagtc ggccgacatc gcgctctacg cggccaagga
     6001 atcggggaag aaccgcgtct cgacggcggg agtcgagtcg agcggctccc tgcgccgccg
     6061 ccgcgatccg cacgggcacg ggccggcgga atcgctttga ctcccctccg cacccggact
     6121 aaggtctgcc cgtgcccgcg acgctccacg accctgcgtt agtcctcggg tcgctcgccg
     6181 ccgtcctcgt tctcgtctat ctcacccggt tcgtcgcgcg cgcgctccac acgtccccgg
     6241 agggtcagcg aaaggcgctc cacgcgctca tcggagcctg gacgctcttc gtcacgcccg
     6301 cgttccatca tctcgcgtgg gcgctggtcc cgccggtcgt cttcggagtt gtgaacgctt
     6361 cggcctgggg gaagaccctc atgcccgatc tcgcgcgaag cgcccacgag tcgcggggac
     6421 tctggacctt cccgctcgcc gtcgtcctgc tctacatcgt cttctgggac gcgccgccgc
     6481 ggtcggccat actcgccggg ctcggcgtgc tcgcgctcgc ggatccggtc gcgtcgctcg
     6541 tcggcgcgcg atggggccag cggaagctcg tctggggcgg tggacgcacg ctcgagggaa
     6601 cgctcgcgtt cctggtcgtg gcgtcgatcg tggtcggaac gattgcggcc gtgacgggcg
     6661 cgcagggcgc gttcccgtgg cggatggggg tcggatgcgg ggcggtcggc gcgatcgtgg
     6721 aagcgtggac gcccgcggga tgggacaatc tcacgatccc gctcgcggtc gcgggaacat
     6781 accacctgct ggcgtgagct gaccgtgatc gccccgacgc cgaccacgat cgcgctcggt
     6841 ctcgcgtgcg ccggagcggg cctcgtcgcc acgacggggc agggcacgtg gcgcggcgct
     6901 ctggccggcc tcgccatcgc gttcgtggct attctgggac tcggcccggg cgcgctcctc
     6961 ccgctcggga tcttcgtgct cggctcgggg gcgctgacgc gcatcggccg cgcgcgcaag
     7021 gaggccgcgg gcgccgcgga agcgcatcgc ggcaggcgcg gcatcgcgaa cgtcgccgcg
     7081 aagctcggac tgcccgcatt cctcggagcg gtcggactcc tcgtcgaccc ggcgtccggg
     7141 gccatggagc tcgtccgcct gatgtacgcg gcggcgctcg ccgcggcgtt cgcggacacg
     7201 gcggcgaccg aagtcggccc gatcgcgcgg ggaccggtct gggcgctcca cgggctccgg
     7261 ccgaagcgcg tggagcacgg aacaccggga ggtgtgagtc aggcaggagc gttgacgtcg
     7321 gtcgctgccg cggcgctcgt gggatgggct gcgagccggt cggacctgat tctgccgtcg
     7381 cagtggccgc tcaccgccgc ggctggggtc gtcgcaactt gggtggagag tgcgctcgcg
     7441 gggacggcgc tgggacgctc gctcggacat cacgggcgaa acgcgtgtct ttccgccgtg
     7501 gccgtgtcgc tcggcggggt agcatggatc gcaatggggg ggcatcggtg atcgagatcg
     7561 tttgcgtggt gggcgcgcgt cccaatttca tgaagatggc gcccattctg gacgcgctcc
     7621 gggcctatcc ggacatcaag cccttcctcg tccacacggg ccagcactac gacgaggaga
     7681 tgtcgcgcgt cttcttccag gagctcgggc tcccgcgtcc cgaccgcgac ctcgaggtgg
     7741 ggagcgactc gcacggccgc cagaccggga agatcatgat ggccttcgac agcctgctcg
     7801 acgggcggtc gacgcgtctc gtggtcgtgg tgggggacgt gaactcgacc gtcgcgtgcg
     7861 ggctcgttgc ggtgaagcgc ggcatcgcgc tcgcccacgt cgaagcagga cttcgaagcc
     7921 gcgatcgcac gatgccggag gagatcaacc gccttgtcac cgacgcgatc tcggatctcc
     7981 tcttcgccac ctcgcccgac gccatcgaaa acctgcgcgc cgagggtgtg cccgactcca
     8041 agatccacct cgttgggaat ccgatgatcg actccatcca ccgccatcgc gaggccgccc
     8101 gcgcgcgaaa cgttcccgcg cggttcggtc tcgcgcagag gcgctacgga ctcgtcacgc
     8161 tccatcgccc gagcaacgtc gacgatcccg gcacgctcgc cggcatcctc gagagccttc
     8221 aggccctggc caagcgcgcc ccgatcctct tcccggtgca cccgcgaacg cgggagcgca
     8281 tccggtccct cggattccag ccaggccccg gcctcgcctt gaacgaaccc ctcgggtatc
     8341 tggacttcct cgggctgatg gatcaggcta cgtttgtcct gacggacagt gggggaatcc
     8401 aggaagagac ggccgcgctc ggagttccat gcctcacgct tcgagccaat acggagcggc
     8461 cgatcacctt gaccgagggg acgaaccgtc tcctcggcac cgaccctcgc gccatcgtgc
     8521 ccgccgccac ggcgcttttg gacggggatg cacccgcccc gaagtgcccg gcgctctggg
     8581 atggccatgc cgggcaacgg atcgccaagg tgcttgccga tcatgtgaac cagggcgcct
     8641 ccgcggagaa tcgaaagggc gtccaagttg acacctaacc gtagctcttg ctacgattaa
     8701 ggactttcat gaacgtaccg acgcagcttc gaggtcgccc agaacgggca cggaagggcc
     8761 cgctcccctt ggcgtccgcg ctgttggccg tcctgttcct ggtttctctc cccgctgtcg
     8821 tcctggcgca gcgcagcgac acgccccctc cgggtgatcg gaactacaag atcggcatcg
     8881 gggatcttct caagatcgag gttgtcggac ggccggacct ctccgggcag atggaggtcg
     8941 ggatcgatgg gcgtgtcacg ctgcccgtcg tgggagccgt caaggcggcg gatcgcacca
     9001 ccgccgagct gtcgaatgat ctatcgcgtc gcatctcgtt ggttcagcgc gtcgcgcccg
     9061 aggtcatcgt ctccgtgctc gagtaccgga gccggaaggt gttcgtgctc ggctccgtcc
     9121 tgatcgcggg aacctattcg atcggggcca cggcgatgag cgtgtgggat gcgattgcgg
     9181 aggctggggg gcctacggaa gacgccaacc tgagcgccgt cgaggtcatt ccgggggaca
     9241 tatccggcgg ttcgcaggtc cggaccgtgg acgtggccgc cgcgattcgc gacggcaagc
     9301 tcgacgacct cgagccgctc cggccgggcg atacggtccg cgtgccgagg ggcggaggat
     9361 ccgcggcggg catcggcaac atcgtctaca tcttcggcgc cgtgggtgcg cagggcccgc
     9421 acccgttcga tccgggcatg ggcctcgtgc aggcgctcat ccggagcact ccgtccgcgg
     9481 acgcgaattt ctccaagatc gagatcgttc ggaatgcggg tccgcgaatc atgcgtatgg
     9541 agatcgacct gaacgagtac ctaggacacg cgtccctgac cggcaatccc gagctccagg
     9601 cgggggacac gatcttcgtg ccgcgatccg ggcggggctt tgcgggcgtc ggctggctgg
     9661 ggctcctggg agtcctgggc acggtgctcg gcctcgtcgt tgccgtggac gccatcgcga
     9721 acaacaacca ctgaccggat ggccatggat ccgcacatgc cgcacccgta tcccgctccg
     9781 tatccgcagg cgccgccgcc cgagaagcag cccgtcttcg atatccgcga cgtcctttgg
     9841 cgtgtcaccc ggtaccggtg gctgctggtc gtgcccatcg tgatctcgat gagcggcgcg
     9901 gccatctatt acacactggc ccccgagatc ttcgagtctc acgtgctcgt ggcgatcgac
     9961 gaccaggcgc aggtgtcggg tgccctccag gggctggtcc agcccgaccg ggaccgggac
    10021 acgccgcggg agcgcgtgac cctcgtcgac agcaaggtcc ggagccgccg tttcctccag
    10081 gagctgtccc ttcgctcggg attcaatcga gacccccagg tcgtcaatga cgccgaaaga
    10141 gccaaccggc gttggcgtgg agtgtcgagc gaggagttcg cgatccgctt cgcgagcgcc
    10201 cgcttgagca agcggatccg cgtgacgccc gtcgagggaa cctacgtccg catttcggcc
    10261 atgggccctt cccccgggga ggcgcgccgg attgcgagcc tgatcgcgga catcctcatc
    10321 gaggagagca agcggacgaa cctcgagcgc gtccaggcgc gcggcgagtt cagcgcggac
    10381 cagatcaccg tctacgaaga gcgcctcaga aagtccgagg acgcctacca gcgctatcaa
    10441 gagtcgctga tccgaaacgt gctggcgagc aatccggtca acgaccggaa catcgaggac
    10501 gcacgcggcc tcatcaacat ggccgagagt gagatggccc aggtgaagca gcgcctcgaa
    10561 agcgagcagc gggacttcgc gggcgcgtcc aacggcgcct cggtgcccag ccttcagggg
    10621 ggcaaggcga tcgagctgtc gaaccgcctc gtggagctcg aatcctcggc gagcgcagcg
    10681 gcgctcagcg acaaggagaa gtcggaccgg aacatcgcgg gcatccagat gagcatcgcc
    10741 tccacgcgcc agatgcttct ggccgagctc gagctcgaag cgagccagct tcagggcaat
    10801 ctcagcgacg aggcgcagtc cgcggccaca ggcatcgcgc tggaccgcgc catcatccgc
    10861 tcgctcacct tgaagcggga ccgcctccgc ggtctcatct ccgattatgc ccggaccgtg
    10921 cagagcacgc cgcgcgagga catggagctt gcgaggctca agggcgaggt ggatacgaat
    10981 cgcgatctct tgaacgcact ccgtcgcgag cagacctcgt ctcgcatctc cgaagcgctc
    11041 gagacgagcg gcctcggatt ccggctctcg gtgctcgagc cgccgcagct tccgtaccga
    11101 gcgtcggagc ccgacccgtt caagatcttc gcagcggccg cgctcctcgg gccgctcggc
    11161 gccgcaggca tcgtccttct gggtgagaag gtccggctcg tcgtccgctc ggtcgagcag
    11221 gcggaagggg agctgggcgc ccgcgtgatc gcgaccttcc cgcggatcga gggctggtcg
    11281 cgccccggta cctatttcca gaacaactgg ccggtgatgg tcatcttcgc cgtcgtcctc
    11341 gtgacgggga cgttctttgc gctgaacgcg acgctcttcg cgccccatcc agtcgtcaag
    11401 aagacccaga ccaagaagta gcgaggatca tcgtgaagcg tccgcagcga accctgttcg
    11461 acagcttcaa tctggatgcg cctcatgtga ccgaggcgcg gcgcctcttg cagacgctcg
    11521 cgcggaggga gcgggacgaa tcgaacacgc cgaaggtcta catggtgacg agcgccggcc
    11581 gcggggaagg gaagagcacg atttgcgggc tcgtctccat ggtcgcggcg cgcatcttcc
    11641 ggcggaagac gctggtgatc gacgccgacc tcaggcgccc gtcgatgcac tcgttgctcg
    11701 gtttgaacca gaggcccggc ctggccgagc tgatggaagg atccgcgtcg gtcgaggaag
    11761 ccacccggtc gacgctcata ggcaccctgt ccgcgattcc gagcggaagg ccgaccaaat
    11821 ccgtggggga cacgtatgac gatttcgcgt ttcgcgagct cctggaccag ttccgtccgc
    11881 agtacgacct catcttcatc gactgcccgc cggtcgtgcc cgtgatggaa ccgctcctga
    11941 tcgcggagca cgtcgacgcg ctcctcctcg tggccatggc cggcgcgacg caggtgacga
    12001 tgatccgccg catgcgacag atcctggcgc cgctcgaacc gaagatcgcg ggcgccatcc
    12061 tgaacaacgc cacggacggc ctcccctact actacgacta ccgctattac gggtacgagt
    12121 cgcccaagcc gaaacgagtg cgcgagattc gcacccctgc gaagccttcg tcgcaggacg
    12181 atgccgcggc gcggcaggtg gcccgcgagc ggggaggtgc ctgatggccg gacggaacgg
    12241 ctcgcgctcg gcggcacgaa cgaagcggag cggagggagc ccgctgcccg gcttcgaagc
    12301 gaaggtgcag aacggcacgg cccggatcgg aatcatcggg ctcggctacg ttggacttcc
    12361 gctcgcggtc gagttcgcga agaagggctt ccgcgtcacc gggttcgaga tcaacaagaa
    12421 gcgcgtcggc gtcctcaact cgggtcgctc ctacatccag gacgtaccca cggccgaggt
    12481 gcgggagctg gtcaagagcg gaaagctcca cgcgacgctc gatttcaacg agctcaaggc
    12541 gatggacgtg atcgacatcg cggtccccac gccgctccgg aagacgaagg atccggacgt
    12601 ctccttcatc gtggccgcgg tcagcgagat cgcgcccagg ctgaagcggg gacagctcgt
    12661 cgtgctggag agcaccacgt atcccggcac gacggacgag ctcgtccgtc ccatgctgga
    12721 agagggtggt ctcagggcgg gacgtgattt cttcctcgcc ttctcgcccg agcgcgtgga
    12781 cccgggcaat ccgacgtatc agaccaagaa catcccgaag gtggtgggcg gcacgtcggc
    12841 cgactgcacg cgcgcggccg cgctcctcta tggcacggtc atcgaccgga tcgtcccggt
    12901 ccagtcgacg caggtcgccg agacggtcaa gctgctcgag aacaccttcc gcagcgtgaa
    12961 catcggcctc gtgaacgagg tcgcgctcat gtgcgacaag atgaagatcg acgtctggga
    13021 agtgatcgat gccgccaaga cgaagccgtt cgggttcatg ccgttctatc ctggccccgg
    13081 actcggcggg cactgcatcc cgatcgatcc gttctatctc tcctggaagg cgcggcaaag
    13141 cggcttcgag gcccgattca tcgacctggc ggggcaggtc aacgccaaca tgccgcacca
    13201 tgtggcgcgc cgggtcggcg aggctctcaa cacgcgccgg aagtccgtgc gcggggcgcg
    13261 ggtcctcgtt ctcgggatcg cctacaaggc ggacatcgac gacgtgaggg agagcccgtc
    13321 gctcgacatc atcgagaccc tcgcgaacga cggagccaag gtcgagtatt cggacccgtt
    13381 ctgtccctcg ctcgagcaca acggacgtcg gttcacgtcg cgcccgctca cgcccgcgac
    13441 cctacgggct gcggattgcg tggtgatcgc gacggcacac aagatgttcg actacggcct
    13501 gatcgccaag cacgcgcaaa ccgtcgtcga ctgccgcaac gtcctcaaag gacgccgcac
    13561 ccgcggcgtc ttcaggattt aggcgccccg tggcccacta tctggtcacc gggggagcgg
    13621 gattcatcgg ctcccatctc gcggagcgcc tgctcaagga cgggcaccgg gtccgcgtgc
    13681 tcgacaacct ctcgaccggc cgggtctcca atctcgaggc ggtgcgcgcc gcgggacccg
    13741 ccgaccgcct cgagtggatc gaaggcgaca tccgctcgct cgagacctgc cgccgcgcaa
    13801 ccgccggcgt ggactacgtg ctccaccagg cggcgctcgc gtccgtgccg cgctccatcg
    13861 agaatccgat cgactcgaca gccgtgaacg tcgggggcgc gctcaacgtc ttccatgcgt
    13921 ctcgcgacaa cggcgtgaag cgcgtggtgg ccgcgtcgtc ctcgtcggtc tacggcgacg
    13981 ccgcgaccct tccgaagcac gaggagcagc cgaccgcacc cctctcgccg tacgcagcga
    14041 gcaagctcgc gggggagatc tacgcgcgcg tgtacgcggt cacgatgggt ctctccacgg
    14101 tgtcgctccg ctacttcaac gtgttcggtc cgagacagga cccgaactcc cagtacgccg
    14161 cggtggtgcc gcggttcgtg accgcgctcc ggaatggcca gcgccccgtc gtctacggcg
    14221 acggcctcca gagccgcgac ttcaccttca tcgacaacgt cgtcgccgcg aacctggccg
    14281 cctgcaccga gggcccgggc cgtggggagg cgatgaacat cgcctgcggc gaccgctaca
    14341 cgctgctcca gctcctcgac gtgatcggac ggctccttgg cgtcacgccc gatcccgagt
    14401 tccagcccgc gcgcccggga gacgtgaagc actcccaggc gtcgatcgag ctcgcccagc
    14461 ggctgatcgg ttatcgcccg gccgtgggct tcgaagaggg cctcgcccgg accgtcgagt
    14521 ccttccagcc ggccggctga tgaccgcgcc gaccacggcg gtcgcgtccg tggcgatcgc
    14581 ggaggcgccg ctcgacggca ccggttgggc cgactacgcc gccgcgcatc ccgccgcgac
    14641 cttcttccat caacccggtt ggctcgaggt cgtgcggagg acgttcgggt accggcacgc
    14701 ctccatcctg gcccgccgcg acggtcgcgt tgtcggtttc ctcccactct tcctggtgcc
    14761 cacgcttcca tggggccgct cgctcatctc gacgccccag gcggtctacg gcggcccgat
    14821 cgcggacgac gacgaggcgc gctccgctct ggtcgatcat gcggcgcggc tcgcggaagc
    14881 ccgcggcgcc tcgttcctcg agctccggaa cgagaccccg atccctgggc tcgcggggaa
    14941 ggacctctat gtcaccttcc gccgccccat ccatcccgac gccgagcaga acatggcggc
    15001 cattccgcgc aatcaaagac gcagcatccg cgtcgcacag aagcacggtc tcaccgcgcg
    15061 cgtgggccat ggcgacctcc tggacggctt ctaccgcgtc tactccgaga gcgtgcgcaa
    15121 tctgggaacg cccgtcttcc cacgcgcgct tttcgcgaac gtgctggagc gcttcggcga
    15181 ggacgcgttc attttgggta tctacaaggg agatcaaatg ctgtcggcgg tgctcacgtt
    15241 ccgctaccgc gaccagatcc ttccgtacta cggcggcgcg acccgcgagg gaatccagta
    15301 tgcgacgaac gatttcatgt actggagcct cctcctgaac gggatggagc gcggcctcgg
    15361 cgtcttcgac ttcgggcgga gcaaggtcga cagcggctcg tatcacttca agcgccactg
    15421 gggcttcgag cccaccccgc tcgcctacca gtaccacctg gcgccgggga aggcgctgcc
    15481 ggacttgagc ccgcgcaatc caaagttctc gatggcgatc cgcgtgtggc ggaggctgcc
    15541 gctcaagctc acacagtggg tcgggccgca cctggtgcgc tacttcccgt gaacatcctc
    15601 tatctctgcc accgcattcc gttccccccg gacaaggggg acaagatccg ctcctaccat
    15661 cagatcgaat cgctcggggc gcgccatcgc gtgcatgtcg cgaccttcgt cgattcgccc
    15721 gaagacgagc ggcacgtacc cgagctcaag gagcggtgcg cgagcctcgt cgtggaccgg
    15781 cgcaccaccg cgaccaacct cacgcgtgcc gcgggagcgc tcctcacggg cgatcccttc
    15841 tccgtggcct gctttcggtc ctcgcgcatc gctcgcggcg tggaccgggc gctccacgaa
    15901 gagggaatcg acctcgcgct cgtcttctcg tcgggcatgg cgcagtacgt ggagcgggcg
    15961 cagggcatcg cgcgggtcat cgacttcgtg gacgtggatt ccgagaagtg gcgggcgtat
    16021 gccgcgcgcg aacgaggacc gctccggctg atctacgcac gcgaagcgga tctcctcctt
    16081 cgccacgacg cccggatcgc gcatgcgttc gaccgatcgc tcttcgtctc cgagaccgag
    16141 gcagccctgt ttcgcgagcg caccggcgcg ggcaacgtgg cggcgattcc gatgggggtc
    16201 gatctcgact acttcgcgcc ggcgcccgag cccgcgacgg agccggtcct cgtgttcgtc
    16261 ggcatgatga gctatatgcc caacgtcgac gccatgcggt tcttcgtggg ggacgtgctg
    16321 ccgctcgtcc ggcgcgaggt cccgaacgtg aggcttcata tcgtggggcg cgaaccggcg
    16381 gcgggagtgc gggatctcgc cgatccgccg cgcgtcgtcg tgactggcgc ggttcccgac
    16441 gtgagaccgt atctgcgcga ggcggccgtc gccatcgccc cgttccgcat ctcccgcgga
    16501 atgcagagca aggtgctcga agcgatggcc tcgggactcc cggtggtggg cacgcggctc
    16561 gcttttcagg gaatcccggc ggggagcgag gacggcgtgg agtcggcaga gggcgcggat
    16621 gccctcgccg ctgctctcat ccggctcctc cgcaatccta cgacccggcg cgatcgcggc
    16681 ggccgcgcgc gccgctacgt cgagcggcac caccgctggg cggaccacgg tgaagcgctc
    16741 gaccatctac tcgaagacgt cgtcgcccga cgccgatcgg agcgaacgcc gctcggcgcc
    16801 ggggctcgaa ccgcgggagg ccgttctgta taattcgtac tcgactcggc ccgattcgcg
    16861 gctcgccgcc gcatggcggc cgtgctaccc actccccaac catcacgatc ggaaccatgt
    16921 gcggcatcgc cggcgcggtt gagaccgccg atccatctcg gcttacggat tccgtcctcc
    16981 agcggatggc gtcgctgatc gcccaccgcg gcccggacaa tcagggcgcg tggctcgcgc
    17041 cgcccgccgg gctctgccat cggcgtctct cgatcatcga tctgagcgag gcgggcaacc
    17101 agcccatgtc gctcgagggg accggcctca cgatggtctt caacggcgag atctacaact
    17161 tcccggagct tcgttcggag ctggaaggga agggggtcgt cttccggtcg cgctccgaca
    17221 cggaggtgct gctccgcgcg tacgaggcct gggacgtcgg ctgcctcgac cacatcaacg
    17281 gcatgttcgc cttcgccatc tgggacgcac cgcgacggcg gctcttcgcc gcacgcgacc
    17341 ggctggggaa gaagcccttc ttctaccacg ctcgccccgg ccgcttcgtc ttcggatccg
    17401 agatgaagtc gatcctcggc tacggcggca tcgaccgcga catcgacccg cacgcgctgg
    17461 acgagttcct ctcgaccggc tgcatcggcg cgccgcgcac gatcctccgt tcgatccgga
    17521 agcttccgcc cgcgcactac ctcctgctcg aaggggatag gatcacgatc cgcccctact
    17581 gggacgtcac gttccgcgtc accgagcccg agcggaccga aggggagcgg atcgagcggc
    17641 tcgagcatct cttccgcgag gcggtgcggc ggcggatgat cagcgacgtc ccgctcggag
    17701 cctttctctc gggaggcatc gattcgagcg cggtggtcgg gatgatggcg cagctctcgg
    17761 accgtccggt gaagacgtac acgatcggct tcgaggagaa gggctactcg gagatcgagg
    17821 acgcccgcgt gatcgcgcgg cacttcggca ccgagcatca cgagttccac gtgcggccgg
    17881 aggcgatcgg gatcctgccc gacctcgtct ggcacttcga cgagcccttc ggcgattcgt
    17941 ccgccatccc cacgtactac gtctccaaga ttgccgccga gcacgtgaag gtcgcgctcg
    18001 cgggggacgg cggggacgag ctcttcgcgg ggtacacgcg gtaccaggac tcgctccgcg
    18061 tcccgcccta ccacctcatt ccggcctcgg tgcgccgccg cgtgctcgcg cccttggccg
    18121 accggatgcc gctggccgcg cccgggcgga accggctcta cgccgcggcc caccacgcgc
    18181 cgctcacgcc cggttatggg ctcgggctct acccctacat caaggaacag ctcctgagcc
    18241 cggagtggcg cacgcagacg gcgctccgcg agccgaacgg gcacaccgcg gtgagtgagc
    18301 cgcgtcttcg ctcgatggat ctcctgtcgc ggctccagta catcgacacg aaggtctacc
    18361 tgccggagga catcctcacc aaggtggacc ggatgagcat ggcccactca ctcgaggtgc
    18421 gagcgccgct cctcgactac acgctgatcg agtacgtggc ctcgattccg cccgcgctca
    18481 agctgcgcga ctcggtgtcg aagtacctgt tccgcaagat ggcgtcccgc tacctgccgg
    18541 cttcggtctt cgagaagccc aagcaaggct ttggcgtccc gaccggcgcc tggttccagc
    18601 aagagcttcg ggtcagcgcg cgcgagcggc tgctcgaccg gcggacgctc gaccgcggct
    18661 acttcaggcg acgcacggtc gagcgcgttc tggatctcca cgaggcgggg cagcgggatt
    18721 acggaacctg gatctggtgc ctcctcgtcc tcgaggagtg gcaccgtatg ttcctcgacg
    18781 cagacacgcg tcgcgtgtag gcgggagccc gacaccatgt gtggaatctg cggcatcgtc
    18841 gactttcgaa gccccccgtc cctggagctc ctggggcgga tgacgaacgc gctccgccat
    18901 cggggcccgg acgggaacgg ctgctatcac gacgggccgg ccgccctcgg ccacgcgcgg
    18961 ctcgccatca tcgacctcgt caccggcgac cagcccatgt ggaccgagga tcggtctctc
    19021 gccgtcgtgc tgaatggcga gatctacaac ttccgctcgc tccggaaggc cctcgaggct
    19081 cgcgggcaca cgttccacac gcagagcgac accgaagtca tcctgaaggc gtatgaggag
    19141 tacggcgagg actgcgcgcg ccatttgcgc ggcatgttca cgatcgcgct ctgggaccgg
    19201 cggcgccagt gcatgttcct cgcgcgcgac cgcgtcggga tcaagccgct ctactacgcc
    19261 tgggatggcc accggttcct cttcggatcc gagatcaagg ccctcctcga gtcgcccgac
    19321 ctggaccgca ccgtcgaccc gctcgcgctc gacgactacc tgacctatct ctacgttccg
    19381 gcgccgaaga cgatcttcac gcacgtgcgg aagctgccgc cggcgcacac gatgcgggtg
    19441 gaccgggatg gactccacgt ccgggagtac tgggacatct cgttcgagcc gcggcgcgac
    19501 tggacggagc agggcgcggc cgaggcgatc gcggagaagc tgcgagattc cgtggagtgc
    19561 cacctgatca gcgacgtgcc gctcggagcg ttcctctcgg gtggggtcga ttcgagcgcg
    19621 gtggtcgcga cgatggcggg gctcatcgac catcccgttc agaccgcgtc gatcggcttc
    19681 aaggaagcgg cctgggacga gctcccgtac gcacgccagg tggctcagcg gttcgggacg
    19741 cagagtcacg agaggatcgt ggacccgcat gcggccagga ttctcgacga tctcgcatgg
    19801 cactacgacg agccgttcgc ggactcctcg atgatcccga cctattacgt ctccgccgtc
    19861 gcgcgggagc gggtcaccgt cgcgctctcc ggcgacgggg gggacgagaa cttcgcggga
    19921 taccggcggt acaagttcga cgcgttcgag aaccggatcc gcgccgtttt gccgggcgcg
    19981 atccgccgcc cggtcttcgg cacgctgggc cgcatctatc ccaaggccga ctgggctccg
    20041 agaatcttcc gcgggaagac gctcctctcg aacctggcac gggacgcaga gcgcgcctac
    20101 gggcattcgg tgagctgttt cacccccgcg atgaggcaga gcctctatcg ccccgaggcg
    20161 gccgctatgg tgcgcggcta cgactcgcac tcggtgctcc agacgtggtt cgaccgggcg
    20221 agggactggg acccgctctc gcgcgtccag tacgtcgaca tccataccta cctcgtcgac
    20281 gacatcctca ccaaggtcga ccgcgccagc atggcccact cgctcgaggt acgcgtgccg
    20341 ctcctcgatc acgagttcat ggagctggcg gcgtcgatcc cggcggggat gaagctccgg
    20401 aatggtgaag ggaagtacat cttcaagaag gcgctcggga cgacgctccc ggatgacgtg
    20461 atgaaccgtc cgaagatggg attctcgatc ccgatccacg agtggttccg cggagagctc
    20521 aagcccgtgt tcgagtccgc ggtgttcgcg ccggacgcgt tcgtggcgga gaggcttcgg
    20581 accgaggaga tccgccggct ctggaccctg caccagcggg gcgcgcgcga cttctcgccg
    20641 tacctgtggg gcatcctgat gctcgagcac tggggacggc gctttctcag gagatcgggg
    20701 tgaaggtcct cgtcttcacc tcgctcttcc cgaacaacgt gtggcccaac caggccgtgt
    20761 tcgtgcggga gcggatcctc cacctggcgc ggatcccggg cgtcgagctc aaggtcgtgg
    20821 cgcccgtgcc ctactatccg ccgcttcccg gttggcgcgc ggcctttcgg cgcgtcgcgt
    20881 cgcgcgagga gcgcgccgga atcgagatcc ttcatccgcg ctacacgatg ctcccgaagg
    20941 tcggatcgcg gtggcacggc gcgttcctgc accgtgcgct tcgtacgcgc atggaacgcc
    21001 tccgccgcga gtttcgcttc gacgtcatcg atgctcacta tctctatccc gacgcctacg
    21061 ccgccgttcg cctcggcagg gagctcggcg tccccgtcgt ctcctccgcg cggggaagcg
    21121 acgtgaatct gatcggccag atgcccgacc tggctccgct cgtgcgtgag gtgctcgtcg
    21181 ggtcgaagcg gctcatcgcg gtctccgcgg cgctcgccca cgcgatgcgc gagatggggg
    21241 cgccggaggt ctcggtgatc ccgaacggcg tggacgcgac gcggttccat ccggtcgatc
    21301 gcgggtcggc tcgccgggat ctcgagctcg gcgaaggacc cgtgatcctc tccgtcggca
    21361 atctcgtccc gaacaagggc atggaccggc tcctccgcgc gctcgcgctc gcgtccgtga
    21421 acccgggatc caccgcgtcg cgagcgacgc tcgtgatcgc gggacgcggg cccgaggagg
    21481 agcggctccg aagactcgcg gagtggcacg gcctccatca tcgcgtccgt ttcacgggcg
    21541 tcctcgcgca cgagtccctg ggtccctggt acgcggccgc cgatctcttc gtgctcgcca
    21601 cggagcggga gggatggccc aacgcgatcc tcgagtcgct cgcctgcggc actcccgccg
    21661 tcgcgacccg cgtcggaggc gtgcccgaac ttctccgaag cgagcgcgtg gggatcatgg
    21721 tcgaaccgag cgacgaagcc ttcgcgcgtg cgatcgagga gggtctcgac cgcgcatggg
    21781 atcgcgcgga gatcgcgcgc ttcgccgccg ggcatacctg ggacgccacg gcgcgttccg
    21841 cccacgcggt gctcgagagc gcgctcgacc gggcgccggc gcgggaggcc cgcgttgcat
    21901 cctgaagctg cgcggtcggc agaggcgatc gggaacgcac tccccgacgg gctcaaggtg
    21961 ctccacgtgc tcgaccacag tctgccgctc cacgcgggct attccttccg cacggcgtcc
    22021 atcctgcacg cccagcgaaa gcagggactc gagcccgtgg ccgtgacgtc gcccaagcac
    22081 gaggagagct ggaagaagga gactcctccg gtcgagacga tcgaggacgt gcgctactac
    22141 cggacaggcc tcgtgccgcc gagccctatt ccgctcgcct acgagatgaa gctgatgggc
    22201 gccctcgagc gccggattcg ggaggtggcg aggatcgagc ggcccgacct gatccacgca
    22261 cattcgccga ttctttgcgc cgtgccggcg ctccgggttg gacgcgccct cgggcttcct
    22321 gtcgtctatg aagtccgcgc cttctgggag gacgccgcgg tcgaccacgg cactgatcgc
    22381 gcgggctcga tccggtaccg cacggtgcgc ggcgtggaga cctgggcttg caagagcgcc
    22441 gaccacgtcg tggtcatctg cggcggaatg aaagatgagc ttctcggacg ggggattcca
    22501 tccgacaaga tcgacatctc ctggaacgcg atcgatcctg gctcgttcca gccaggcgag
    22561 ccggaccgcg ccttcgctcg agagcacggg ctcgaaggga agcgcgtgct cggctttctg
    22621 ggctcgttct acggttatga aggactcgag ctcctcgttt ccgcgatggg cgcgctgcgg
    22681 ggccgccgag aggacctgaa attgctcctg gtggggggcg ggccgcggga acaagcgctc
    22741 cgcgatcaga tccgtcgcga aggattggag caggtggtcg tgatgccggg tagaatagcc
    22801 aaggagcgga tcccggggct ctacgggttg gttgacattc tggtctatcc ccgttattct
    22861 actaggctta cggagctagt gacgcccctg aagccgctcg aggccatggc gatgggcaag
    22921 cctttggtgg ccagcgacat cggggggcat cgcgagctga tccaagatcg ccgcaccggc
    22981 ctcctgttct cgcccggcag cgtgccctcc ctggtttcgg ccgtcgaaac gatcctggat
    23041 gacgacgcgc tccgtgccga tctcgtccgt gggggacgag aatgggtgga gacgaaccgc
    23101 gcctggagca tcacaacctc cgtgtactcg aaggtgtaca cgcaggcgag actccgcgcc
    23161 gaacgcagag gaagagactc gtggaaacga aactcgcagg gcgaagccgc ggaggcgcgg
    23221 tgagccccgc aacatccagc ttcatgcata gggcccctcg cacgatcgcg ctcgtgctcc
    23281 gagcgtcgct cgtcgccttc ctggtcctga tcggtcttgg ctccggcatg gcgtcggccc
    23341 aggagaccca gggggaacag ggtgacgcgt ggctcaaccg caactacttc ggccgccaca
    23401 cggacggatt cacacaggag ctcctggcga acgccgagcg aaatcacctc gcccaggaaa
    23461 atttctggca gaaatacagg tcgggcgagc tcgaccgtgc gctcggagat ttgaaatacg
    23521 tcctcctcgt gttcccgaac catccgaggg cgctccatct gatgacgctc atttgccgca
    23581 cgatgaagga ctccacaacg cccatcgtct acttcgagaa ggcggtgcgc acgttcccga
    23641 acgagccgta cacgttcgct caatacggcg cctatctcat ctcgacaggg gacacggagc
    23701 aggggattgc gcgcctcaag gacgcccttc ggctcgatcc caacttgacg tacgcgctgg
    23761 gcctcttgtc ggaggctcag aagaagccga aggccggaac gacgccgggc gcgggcacga
    23821 caggatccac cgccgcccca ggctcgaagg ccccggccgc cgcgactccg aacgcaaatt
    23881 cgcccaccac ttccgcagca ccttcgacct ccacgcccgg aaccacggtc cgctgaggat
    23941 cgggatcgtc cctcgatgag cggtctctgg ggattcgtcc tcgcggatcc gcgggggcgg
    24001 gctgacatct ccgatctggc cgtgatgggc gcggggcgcc accccggagc cacccatccc
    24061 ctccacgcgg tggctgcagg accggcggcg ctcgccgccc aggagttccc gaatcgagcg
    24121 gcagcggtcg cgcgccgaca ggtcggggac tccattctcc tgctcgccct ccacggatcc
    24181 atcctcgagc tcgacggctc cggctcgacg cacgatggca acgcgcccaa gagcgccaac
    24241 cttctcgcgg accggcttct cgaaggctac ctcggcgcgg gacaggacct ctggcagcgg
    24301 atccgaggag acttcgtcat cgccatctgg gacggccgcg atgccacctt ccgtgtcgcc
    24361 tgcgaccgct ttcggactca ccagtttttc tattccgtgg acatgcgcgg cctcacgttc
    24421 ggctcccgca tcgctccgat cctggcccgt cccggcacgg gaagccccgg catcgactcg
    24481 gattccgtgc tggaggtgct cctcgcgtcg gtcattccga ctccgaaaac gatctacgag
    24541 ggcgtcgcga agctcccgcc gggaacggag cttatctacc ggtccggcac catgaacctc
    24601 cggccgtact gggatatggc gttcggcacc tccgagcgtt cgagcgagcg tgagttggcc
    24661 cgggaccttc gcgaatcact cgaagatgcc atgcgggtcc ggctggccat ggaccgcgac
    24721 gcatcctacg gcgcgttcct gagcggcggg atcgacagca cgacggttct cggcctcgcg
    24781 accaagatcg cgggccggcc ggtgaagagc ttttcgatcg gcttcggcga gcctcgcttc
    24841 aacgaaatgg agtatgcgcg gatcgctgcg cgagccttcg gctcggagca ccacgagtac
    24901 ttcgtccaac ccgaggacgt ggtgcgcgcc atcccgacct tgctcgacgc ctacgacgag
    24961 ccgttcggca acgcgtcggc catcccgacc tactactgcg cgaccgtcgc gcgcgagcac
    25021 ggcatcgacg tcctgtacgc gggcgacggc ggggacgagc tcttcgcggg gaacgagcga
    25081 tactcggtcc agcgtctatt cgactactac cggaaggttc cgagctggct ccgggagccg
    25141 gtcctccggc cgctcgtcga gaccctcgcg gcgacgaagg ttccgctgtt cgtgaaggga
    25201 gcgaagtacg tgcgccgcgc cagaactccc tatgtggacc gaatttcctc gtacgacctc
    25261 gatcacgtcc ttccgctcga cgagatcgtc gcccccgccc agctcgaacg catcacgcga
    25321 cagcacagcc acaaggctct cttgtcgcac tactatcgga gtgcggcggc cgcggacgat
    25381 ctggaccgcc atctctatct cgatctgaag ctcaccatct cggacaatga cctgatcaag
    25441 gtgacgcgcg cgaccgaagc cgccggcgtg gcggtgcgct ttccgttcct ggattcgcga
    25501 gtggccgagg tggcgggcgc cgtgccggcc cgcatccgga tgcgggggac cgagctccgt
    25561 agtttcttca agcgcgccta ctcggatttc ctgccgcccg aaacgattgc caagaccaag
    25621 cacggtttcg gcctcccgat cccggtctgg ctccggaccg atccgacgct gaacggcatg
    25681 atgcgcgatc tcgtgctcgg tccctcgaat cgcgtcgcgg gatattttcg ccggagcgcc
    25741 ctcgagacgc tcgtcgagcg ccacagcacc gaccccacga cgtactacgg cgtcttcctc
    25801 tggaatctca tggtgctcga gctctggctt aggcgccatg cctccaccac gtgaggcccg
    25861 gcccgggagc cggcgccgac acccagccac ggtcgggggt ccgcgtcgaa gtgctccgga
    25921 cggccgagga gctcgatcgt ctcgtgccgg agtgggaggc gctcctcgct cggagcgcga
    25981 gcgacacgat cttcctgtcc ccggactggc tccgctcttg gtgggcggcc tatggatcgg
    26041 ggcgcgagct gattgcgctt cgggtcgtgt cggacggaag cctcgtcggc ctggcgctcc
    26101 tctatcgcgg cctcggacag ctcgtggcgg gcatgcggca tcgcacgctc gtcctgctcg
    26161 gcgacggctc cgcggactcc gactatctcg attggatctc ggcccggggg caggagggga
    26221 cggtcgtcgc aggaatcctc gacggcctcc ggacatcggt gccggggtgg gatctgctcc
    26281 tcctcaacga gatcccggaa acatcgcccc atctcgcccg gctccaggcc gaagctgcaa
    26341 cgcgcggatg gctgtggcat gactggcgcg taccctgcgc gcgcgtgagc ctgccggcga
    26401 gctgggatgc ctacgtcaag tccctcaagc cgcgcatgcg aacgaaggtt cgctcggtgc
    26461 tgcgggtcat ggacgaggag ttccacgcgc ggtacgagcg attgaccgat ctctccgagc
    26521 tcgaggcacg gctccagtcc ctctacgacc tgcatcagcg gcgatgggag gccgacggga
    26581 agtcgggcgt cttccagaca gcgaccaagc gtgacttcta cgcgctcctg tcccgtcggc
    26641 tcctcgagag cgaccgtctt cgcttctatt cgctccggat cgggaccgat tacgtcgccc
    26701 accagtactg cttcgagtat ggtaaccgca tgtacctgtt gcaggagggg ctcgaccccg
    26761 cgtggttcga gcacggtgcg ggcaacgcgc ttcgcgcgca cgtgttccag gactgcatcg
    26821 cgcggggtat cgcggtctac gacttcctgg gcggcgtgac ggcgcacaag ctctcctggg
    26881 gagcggaaac cgtctggagc gcacgcgtga ccaccgggcc gccctcgtgg aagaaccgaa
    26941 tcctgttcgg cgtgcgtgga gcgaaggcgg ccctcaagaa gctcctccgg cccgaacggg
    27001 tcacggagga cgcgggttga cccggaccgt cgtgcgccgc tgggccgcgc gcgtcgcgtt
    27061 tggcttgggc ctgacccaga cgttcacgcg cgctggagat cgctggcgag tgcttcgtgg
    27121 gaacgggggc gtttccgtcg gacggcgccg gcgcggcgcc ttcctcgtgc tcctctacca
    27181 ccgtgtcaac cgggcctccg cgcatttcgc gatcgagcgc acggagacgg aggcgttccg
    27241 cgcccagatg gagcacctcg cacgccattt ccacgtgatg tcgctcgatg atctcgtgaa
    27301 acgcgtcacg gccggcgagt cccttccatc gagagcggtc gcgattacgt tcgatgacgg
    27361 atacgaggac aactacaccg acgcttaccc aatactcagg aaactcggtc ttccggccac
    27421 ggtgtttttg gcgaccgcga gcatcggaac agggaagagc ctctggttcg accgggttct
    27481 cgatggtttc cggaccgcac gggcccactc gatcaagcta ccgggcggcg aatccgccga
    27541 gggattggtc gatccgatga ttcgcgagac gatggcgcat cgtgcgctct acgctctgat
    27601 gcgcctgtcg aacgccgagc gcgagacggc gatcgacaca ctcctgcgaa acctcggccc
    27661 ggagggcccg cccaaccttc ccgcgatgat gacctgggat caggtccggc tcatggcggc
    27721 caatggaatc gacttcggcg ctcacaccat gacccatccg atcctctcgc ggcttcctct
    27781 cgacgaggcc gaagcggaga tcgtcggctc gaagcgcagg atcgaggagg agatcgggac
    27841 accggtccgt ctcttcgcgt atccggtggg acgccgttcc gattattctc cagaggtgat
    27901 cgacctcgta gctcgctccg gattccatgc cgcgctcacg acgactccca cggcgaacct
    27961 ggcgggggac gatccattcc tgctcaaacg catcaagcct ctgggagacg atctgcccag
    28021 ctttgccctc ggcctgagcg cccagtacgt caccgagtgg agacaggccg taccgtgagc
    28081 caggccgcgc cggcgccgga atccatccgc accctcgcga tccgttcctc gttcgtcaac
    28141 ctcgccgcca gtttcgggct caagggtctc accctggccc aggccgtgat ctacgctcgc
    28201 gttttcgtgc cgggagagct cgggcaggtg gcgacggtgc tcctcgtgat ctcgttcatg
    28261 accgtcataa cgcggatggg attcgaggag tcgatcgtcc gggagaacga ccatcccgag
    28321 cgcgtgatgc agaccgcctt cttcctggcg ctcgtgctcg gggccattct ctccgcgggc
    28381 ctctacgtcg cggcccccct cgtcgcgtcg gccttccacc gggcggagct gacccagtac
    28441 ctgcgtttca tggccttcat ggtattcggg agcgcccttg gactcccgaa cgcgctctgg
    28501 gcgcagcgct tccgcttcgg gctggccaag gtcgcctcgt tcgcggatct catcgtggga
    28561 acggtcgtga cgctggtcct ccacttccac gccgggctcg gcatctggag cctcctctgg
    28621 ggacgcatcg cgggcttcgc cgcgaactac ctcacggtct ggatcatcgc ggcgtatcgc
    28681 ccgtggcccg tgttcgatcg ggcccaggcc cgagtccttc tcagcttcgg gtggccgatc
    28741 gttgtcggat ccctggccaa ctacctcatg catcaggggg acgcggtgat cgtccggtac
    28801 tttcacggcg acgcgactct ggcgtactac cagctcgcct atgcgttccc gtattacctc
    28861 atggaaggga cggatgttct tttgggggcg ctacttccaa ccttctccag acttcgggac
    28921 tcccgggaac gcctcgtctc ggccttcgtc cagtccaaca agtacatcac gctggccatc
    28981 gtgccctgtg gcatcgcgct cgcggccctg gccgggccgc tcgttcgcgt cgtgttcggc
    29041 gccaagtggc ttcccgccgt cgggccgatg accgtcctcg cgatcggatt cacggtgcag
    29101 gtcatgtggg gctacggctg gggggccctc gtcctggcgt cgggacggac gcgctacctg
    29161 atgtatgtga agctctggat cgtcgtctac ctcctcaccg cgggaacgct cctgatccgg
    29221 gcgttcgggg cgatgggcgg ggcactctat gtcctgaccc aggccatcct caccgttgga
    29281 gtcgtacggg gctggatcct tcagcacgag ctcggctccc ggagcttcat caaggacagc
    29341 tggaagcccg tggtggcctc cctcgtgccc ggggccctcg tctggtggct cgcgtcgccg
    29401 cacatcgggt cggtggtcgg cctgatcttc accggcatcg cgtatggcgc cgtctacatc
    29461 gggctcatgt ccgtcttcga ccgcgagctc gtccccgagc ttcgccacat ggcgcggctg
    29521 gtcttcgggc cgcgagccgc gtagagtcca accgatgcgc gactacctca tcatggcgtt
    29581 cgtcttctcc atcctgccgt atgcgttcat gcgcccgtac gtcggcgcgc tcatgtgggt
    29641 ctggatcagc atcatgaatc cacaccggct gacctggggc cccgcgtatg gatttccgtt
    29701 cgcgatgctc accgcggtca cgacgatggc gggggtcttc atctcgaacg accggggccg
    29761 ttttccggcg gctcgggaaa cgacgatcat gatcctgatg gcggcctggt tcacgctcac
    29821 gaccacggtg gcgctcatgc ccgaagccgc gtgggcgaag tgggaccggg tcatcaagat
    29881 cctcatcatg acgatcgttc cgctcttcct ccttcaggac aagaatcgcc ttcgcctgct
    29941 ccttctcgtg atggtggcct cgatcgcgtt cttctcggtc aaggggggct tgttctcgct
    30001 cctcaccggg gggcggtacc gggtgtacgg cccgtcccag tcgttcatcg ccgacaacaa
    30061 cgcgctcgcc gtcgcggagc tcatggtgct gccgctgatg ctctatctgg cgaagacgga
    30121 gccgcgcaag tgggtgaagc ggggcctcta cctgtcggtc ccgctcaccc tggtgtccgt
    30181 ggtcttctcc tactcgcggg gcgcgctcct cgggctcctg gcgatggtgg ccgttctcat
    30241 cgtcacgggc cgacgctggg gcctggctgc cgccttcatc gtgctcgcgg gggtgggctt
    30301 cgtgttcatc ccgaaccagt ggttcgagcg catgagcacc atcgggacct acaagcagga
    30361 cgagtccgcc atggcacggt tccagtcctg gggatttgca tggaacgtcg ccaccgaccg
    30421 gcccctcacg ggtgggggat tccgtgtctt ctcgacggac caattcctcc ggtatggtcc
    30481 ggatcccttc gaggcctacg actcccacag catctacttc gaggtcttgg gagagcacgg
    30541 cttcgtgggg ctcatcctgt tcctgagcct gctcgtgtcc tcgttcttga gtctcaactg
    30601 ggtggagcgc cagattcgcg ggcgaccgtc gattgcctgg gcgaaggact acgcggtcat
    30661 gctccggctg agcctcgtgt cgtacgccgt cggcggcgcc ttcctgggtc tcgcctattt
    30721 cgacctctac ttcgacatcg tcgcggccgt cgtcctgctg aagatcctcg tgcagcgcga
    30781 gctggcgcag gaggcggcgg tgagccgggc ctatcgcctg agcgcgccag cgttcgctcc
    30841 tccacgagcc acgtccgcga catgacccgg tgcggacggc tgggcgtggt ccacttgggc
    30901 gctgacgtcc gccacggcgg cgtggtatca tcgttcgccg gcgttgatgt gggagcgccg
    30961 ccccccggtt ccacatctgg agtgacccca tgatccacgg caagcgcctc gtcgtcgtat
    31021 tgcccgcgta caatgcggcc cgtacgctcc agaagaccta cgaagagctc cctcacgaaa
    31081 tcatcgacga cgtgatcctc gtggacgacg cgagcgggga cgagaccgcc gagatggcgc
    31141 gccgcctggg aatccgcacc ttcatccatc ccaagaaccg cggctacggg ggaaatcaga
    31201 agacctgcta tcgcgaggcg ctccagtgcg gggctgacat cgtgatcatg gtccacccgg
    31261 actaccagta caccccggcc ctggtcaccg cgatgtcgtc gctcatcgga atgggcgtgt
    31321 acgactccgt gctgggctcc cgcatgctgg ggacgagtgc gctcaagggt ggcatgccgc
    31381 gctacaagta cgtggccaac cgcatcctga ccttcttcca gaacgtgatg gtcggccgga
    31441 agctctccga gtaccacacg gggtatcgcg ccttccggcg cgaggtgctc cagacgctgc
    31501 cgctcgaaga gaattcggac gatttcgtct tcgacaacga gatgctcacc cagatcatcg
    31561 ccttcggcta ctcgatcggc gaggtga
//
LOCUS       DASTKC010000244         6414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_212531, whole genome shotgun sequence.
ACCESSION   DASTKC010000244 DASTKC010000000
VERSION     DASTKC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212531"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFR25_10310"
     CDS             <1..642
                     /locus_tag="VFR25_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9941478.1"
                     /translation="KRELDRLKAETDRERISLNLIRTERRNLLKKIRSDSKTNEQAAQ
                     ELERASKRIQTLIGELEKRRLAGGRGGPGQEPLIFGDFGQNRGRLPWPVNGRVARGFG
                     SQVNPRFGTKTFNSGVDIAASFGTPIAAVAKGRVEYVNWLEGYGKCAIINHGGGFYTL
                     YANASEILVTVGKDVAAGETIGKVGDTGSTMGTALHFEIRKGREPLNPLDWFR"
     gene            656..1831
                     /locus_tag="VFR25_10315"
     CDS             656..1831
                     /locus_tag="VFR25_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941479.1"
                     /translation="MSHTLRGQDEALSLLRAAVRSGHVGHAYLFHGPAGVGKTRAALL
                     LAQALLCERPATSGEPCGTCSSCKAVLALRHPDLELLVPLPTFRTEGRTERQAEEARS
                     EARAEVFRRMATEPFFTPVFSRPVVHSVEDLARAKQFLSLTAQREGGAKVLIVKGAEA
                     ITGPAAHAFLKILEEPQPRRVLILGTRQPKALLPTIVSRCLLVRFRPLPTAIIGEALA
                     ERGVSKPVARLIAATSQGSLGRALALLEPPLDTTVKEGPGEAEFAKLRAAAVELFVRP
                     KTAAVLGPLRRLRIERDRGRFLTAVSLASYYYRDVLRRKLLGSGAELANADLERDIDA
                     DARALAVPAITERIRILDEIGTAVQSNVTAAYAVAAAQVRMGLGPDEARVRVRPAHA"
     gene            1824..2972
                     /gene="ricT"
                     /locus_tag="VFR25_10320"
     CDS             1824..2972
                     /gene="ricT"
                     /locus_tag="VFR25_10320"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HET9941480.1"
                     /translation="MHEHESDPVIEDVATTPAPPAPGKSGNGAPDAIAADVVELVFKG
                     RRRAYYANPREIPLREGDYVIVQAERGEDLGRVHHTAEWVRRPELYGSLRHVLRPARP
                     EDQETLERNRGKEERAFQICKDRVVQRDIEMKVVDCEYQLDGNRITFYFTAEKRVDFR
                     DLVKDLAAAFRTRIELRQIGVRDEAGRIGGVGTCGRELCCSTWLRQFEPITLKMAKDQ
                     GLSPSPSKISGACGRLKCCLRYELDFYKDVAREFPKVGSKLTLMDGEWEVGRVDIFRR
                     EIHLVTDDGKESMIALAAIPVGTKIIGPSRDRRKGCDKEGCASRGAKPGDGESGPGDH
                     GGARGGDRRPQRPGGDRRGRPGGDRQGGGDRGRGGDRPPRGESPEGRG"
     gene            2969..4492
                     /gene="metG"
                     /locus_tag="VFR25_10325"
     CDS             2969..4492
                     /gene="metG"
                     /locus_tag="VFR25_10325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9941481.1"
                     /translation="MSKFYITTAIDYVNNVPHLGTAYEKIAADAIARYKRLLGFDTRF
                     LMGNDEHSTNVEKAAKEQGLDPKAYCDKMAEIFQGVWKRLDISYDDFIRTTEPRHVRG
                     VQALFEKIASTGDIYKGKYEGFYCVSCERFYPEKDLIDGKCPDHKTPAVFLTEDNYFF
                     KLSKYGPPLLEHIRKNPEFIVPEIRRNEIVNVIEGGLEDISVSRASGTWGIPLPIDAS
                     QTVYVWFDALINYITGVGYPETNGNFQRYWPADLHVIGKDITRFHCIIWPAMLMSAGV
                     PLPKKVLGHGWVHFQGEKLSKSLGNIVNPLDIADKFGADSLRYYVLKEVPLWRDGDFT
                     WDLFIQRYNSDLANDWGNLFTRTVSMIHRYRGGKLASGEAGDGVGDVIREALREARAA
                     VDRYEIEGAIEAAWTIIRRANRLVEERAPWNLAKDPARADDLDRLLGTLAVALQHTAL
                     LLFPIMPSKAVQVWDTLRLTPALADDARFPTDGVLLPPPPIGVEMGESKPLFPRIER"
     gene            4489..5250
                     /locus_tag="VFR25_10330"
     CDS             4489..5250
                     /locus_tag="VFR25_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9941482.1"
                     /translation="MIDSHAHVGRPEFDSDRDQVLARAREAGVQLVIEAGTDARSSRA
                     AIELARRVPGVRAAVGFHPCDVAGDRIGEMAEIEALADEPEVVAIGETGLDAHWPDNA
                     PAEVQEEFLKRHIALSKRVGKPLVLHHRKAGERVAEVLEEVGPPDAGGTFHCFAGDAA
                     LARRVIAMGFRIGIGGSATFKKSPLPELVREVGVEHVILETDSPYLAPTPHRGKRNEP
                     AYLVLVRDQVAGSLGIAPEALEKATDASARALFRI"
     gene            5219..6145
                     /gene="rsmA"
                     /locus_tag="VFR25_10335"
     CDS             5219..6145
                     /gene="rsmA"
                     /locus_tag="VFR25_10335"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9941483.1"
                     /translation="MPRLALSSGSRGTELKERAPRDAGAARAAGAATSGVPAALRAIG
                     VRPSRRLGQNFLIDPRVAERIVALVEDPREAVIEIGPGLGALTTLLAATGRELTAVEL
                     DLRLAEALETRLAVWPAARVVRGDILEQRAEDLASGPATVVANLPYSITTPALEWIVT
                     QGGRVKRAVLMVQREYAQRLSAKPGGKEHGSITVFLQLHAEVRVLFRVSPGAFHPRPE
                     VDSVVLEVVPRPYPGTTAEERASVERLARAGMGTRRKTLANALGRALGMEGAVVRAWL
                     GAAGVDPERRGETLTVDEWIALARVAPRRMTT"
BASE COUNT         1056 a         2128 c         2246 g          984 t
ORIGIN      
        1 aagcgcgagc tggaccggct caaggccgag accgaccgcg agcggatctc gctcaacctg
       61 atccggaccg agcggaggaa cctcctcaag aagatccggt ccgattccaa gacgaacgag
      121 caggccgccc aggagctgga gcgcgcgagc aagcgcatcc agaccctgat cggggagctg
      181 gagaagcggc ggctggccgg cgggcgaggg gggcccggtc aggagcccct catcttcggg
      241 gacttcgggc agaaccgggg acgcctcccc tggccggtta acggacgtgt ggccaggggc
      301 ttcgggagcc aggtgaaccc gcgcttcggg accaagacct tcaacagcgg cgtggacatc
      361 gcggccagct tcggaacccc gatcgcggcc gtcgccaagg gccgggtgga gtacgtcaac
      421 tggctcgagg ggtacggcaa gtgtgctatt atcaaccacg gtggaggctt ctatacgctg
      481 tacgcgaacg cctccgaaat cctggttacg gtcggtaaag atgtagccgc cggtgaaacg
      541 atcggcaagg tcggggacac cggctcgacc atgggtacag ccctccattt cgagatccgg
      601 aaggggcggg agccattgaa ccccctcgac tggttccgtt gagtcttcga ataccatgag
      661 ccacacgctg cgcggccagg acgaggcgct gagcctcctc cgagccgccg ttcgttccgg
      721 tcacgtcggg cacgcctacc tcttccacgg ccccgccggg gtggggaaga cgagggccgc
      781 cctccttttg gctcaggcgc tcctctgcga gcgtcccgct acgagtggcg agccctgcgg
      841 gacgtgctcg tcctgcaaag cggtcctcgc gctccgccat cccgatctcg agctcctcgt
      901 tccgcttccc accttccgga ccgaaggccg caccgagcgc caagccgagg aggcacggtc
      961 ggaagcgcgc gcggaggtgt tccgtcgcat ggctacggag cccttcttca cgccggtctt
     1021 ctcgcgcccg gtggtccatt cggtcgagga cctcgcgcgc gcgaagcagt tcctgtcgct
     1081 caccgcgcag cgggaaggtg gcgcgaaggt cttgatcgtg aagggggccg aggcgatcac
     1141 gggcccggcg gcgcacgcat tcctgaaaat tctcgaggag ccgcaaccgc gccgcgtgct
     1201 catcctgggc actcgccagc ccaaggcgct ccttccgacc atcgtgtcgc gatgcctcct
     1261 cgtgcggttc cggccgctcc cgaccgcaat catcggcgag gcgctcgcgg agcgcggcgt
     1321 gtcgaagccg gtcgcgcggc tcatcgcggc cacgagccag gggagtctgg ggcgcgcgct
     1381 cgcgctgctc gagcctccgc tcgacaccac ggtcaaggaa gggcccggcg aggccgagtt
     1441 cgccaagctc cgcgccgccg cggtcgagct cttcgtgaga ccgaagacgg ccgccgtgct
     1501 cggtcctttg aggcgcttgc gaatcgagcg cgatcgcggc cggttcctga ccgcggtgtc
     1561 gctcgcgtcc tactactacc gcgatgtgct gcgccggaag ctcctcggct ccggcgccga
     1621 gctcgcgaac gccgacctcg agcgggacat cgacgccgac gcccgcgcgc tcgccgtgcc
     1681 ggccatcacc gagcgaatcc ggatcctcga cgagatcggg accgccgtgc agtcgaacgt
     1741 caccgcggcc tacgcggtcg cggcggcgca agtgcggatg ggactcgggc cggacgaggc
     1801 ccgcgtgcga gtgaggcccg cccatgcatg agcacgagtc cgatccggtg atcgaggacg
     1861 tggctacaac tcccgcgccc cccgcgcctg ggaagtccgg caacggcgct cccgatgcga
     1921 tcgcggccga cgtcgtcgag ctcgtcttca agggccggcg ccgggcgtac tacgcgaacc
     1981 cgcgcgagat cccgctgcgc gaaggcgatt acgtgatcgt ccaggccgag cgcggcgagg
     2041 acctgggacg cgtgcaccac accgcggagt gggtccggcg tcccgagctg tacggaagtc
     2101 tccgtcacgt gctccgccct gcgcgccccg aagatcagga gacgctcgag cggaaccgag
     2161 gcaaggagga gcgcgcgttc cagatctgca aggaccgcgt ggtccagcgc gacatcgaga
     2221 tgaaggtcgt cgactgcgag taccagctcg acggcaaccg gatcacgttc tacttcaccg
     2281 ccgagaagcg ggtggacttc cgcgatctcg tgaaggacct cgccgccgcg tttcgcaccc
     2341 ggatcgagct ccggcagatc ggcgtgcgcg acgaggcggg gcggatcggc ggcgtcggga
     2401 cctgcggccg cgagctctgc tgctcgacgt ggctccgtca gttcgagccg atcacgctca
     2461 agatggcgaa ggaccagggg ctctcgccga gcccgtccaa gatctcgggc gcgtgcgggc
     2521 ggctcaagtg ctgcctccgg tacgagctcg acttctacaa ggacgtggcg cgggagttcc
     2581 cgaaggtcgg gagcaagctc accctcatgg acggcgagtg ggaagtcggc cgcgtggaca
     2641 tcttccgccg cgagatccat ctcgtcacgg acgacggcaa ggaatcgatg atcgcgctcg
     2701 ccgcgattcc ggtggggacc aagatcattg gaccctcgcg cgatcgcagg aagggatgcg
     2761 acaaggaagg ctgcgcctcg cgcggcgcga agcccgggga cggcgagagc ggacccggcg
     2821 atcacggtgg cgcgcgcggc ggcgatcggc ggccgcagcg tccaggcggt gatcgtcgcg
     2881 gacgtccggg gggcgaccgc cagggcggcg gtgaccgcgg ccgtggcggt gatcggccgc
     2941 cgcgagggga gtcaccggag gggcgcgggt gagcaagttc tacatcacga cggcgatcga
     3001 ctacgtgaac aacgtcccgc acctcgggac cgcgtacgag aagatcgccg ccgacgccat
     3061 tgcccggtac aagcgcctcc tcggattcga cacgcggttc ctcatgggga acgacgagca
     3121 ctcgaccaac gtcgagaagg cagcaaagga acaggggctc gatcccaagg cctactgcga
     3181 caagatggcc gagatcttcc agggcgtctg gaagcgcctc gacatctcct acgacgactt
     3241 catccggacg accgagccgc gccacgtgcg tggcgtccag gcgctgttcg agaagatcgc
     3301 gtcgaccgga gacatctaca agggcaagta cgagggcttc tactgcgtca gctgcgagcg
     3361 gttctatccc gagaaggact tgatcgacgg gaagtgcccc gaccacaaga cgccggcggt
     3421 ctttctgacc gaggacaact acttcttcaa gctctcgaag tacggcccgc cgctcctcga
     3481 gcacatccgg aagaatccgg agttcatcgt gccggagatc cgccggaacg agatcgtgaa
     3541 cgtgatcgaa ggcggactcg aggacatctc ggtctcccgc gcgtcgggaa cgtggggcat
     3601 cccgcttccg atcgacgcga gccagaccgt gtacgtctgg ttcgacgccc tcatcaacta
     3661 catcaccggc gtcggctacc cggagacgaa cggcaatttc cagcgctact ggccggccga
     3721 cctccacgtg atcggcaagg acatcacgcg cttccattgc atcatctggc ccgcgatgct
     3781 catgagcgcc ggcgtgccgc tgccgaagaa ggtgctgggg cacggctggg tgcacttcca
     3841 gggggagaag ctctcgaagt cgctcgggaa catcgtgaat ccgctcgaca tcgcggacaa
     3901 gttcggcgcg gactcgctcc ggtactacgt cctcaaggaa gtgcccctct ggcgggacgg
     3961 cgacttcacg tgggacctct tcatccagcg ctacaactcg gacctcgcga acgactgggg
     4021 caatctcttc acgcgcacgg tctcgatgat ccaccgctac cgcggaggga agctcgcgag
     4081 cggcgaggcg ggtgacggcg tgggcgacgt gattcgcgag gcgctgcgcg aggcccgcgc
     4141 ggccgtggat cgctacgaga tcgaaggggc gatcgaggcg gcgtggacca tcatccgccg
     4201 cgcgaaccgt ctcgtcgagg agcgcgctcc ctggaatctc gccaaggatc ccgcgcgcgc
     4261 cgacgacctc gaccggctcc tcggcacgct tgcggtcgcg ctccagcaca cggccctgct
     4321 cctctttccg atcatgccgt cgaaggcggt ccaggtctgg gacacgctcc ggctcacgcc
     4381 ggccctggcc gacgatgccc ggttcccgac ggatggcgtg ctcctgcccc cgcccccgat
     4441 cggcgtcgag atgggggaat ccaagccgct cttcccgcgc atcgagcgat gatcgactcc
     4501 cacgcgcacg tggggaggcc ggaattcgac tcagaccgcg accaggtcct ggcgagggcg
     4561 cgcgaagccg gtgttcaact cgtcatcgaa gccggaacgg acgcccgatc cagccgcgcg
     4621 gccattgaat tggcccggcg ggtgccgggc gtccgcgccg ccgtcggctt tcacccatgc
     4681 gacgtggccg gcgaccggat cggggaaatg gccgagatcg aggcacttgc ggacgagccc
     4741 gaggtcgtcg ccatcggcga gaccgggctc gatgcccatt ggccggacaa cgcccccgcg
     4801 gaagttcagg aggagttcct gaagcgccac atcgccctgt ccaagcgggt cggaaagccc
     4861 ctcgtcctcc atcaccggaa ggccggggag cgcgtggccg aggtcctgga ggaggtgggt
     4921 ccgcccgacg ccggcggcac cttccactgt tttgcgggcg atgccgccct ggcccggcgc
     4981 gtgatcgcga tgggattccg gatcgggatc ggcggctccg ccacgttcaa gaagagcccg
     5041 ctccccgagc tcgtgcgcga agtcggcgtg gagcacgtca tcctcgaaac ggattcgccc
     5101 tacctcgccc ccacgcccca tcggggaaag cgaaacgagc ccgcgtacct cgtgctcgtg
     5161 cgcgatcagg tggcgggatc tctgggaatc gcgccggaag cgctggagaa ggcgaccgat
     5221 gcctcggctc gcgctctctt ccggatctag gggcacggag ctgaaggagc gcgcgccgcg
     5281 ggacgctggt gccgcgcgcg cggccggtgc agccacctcc ggcgtgcccg cggcgcttcg
     5341 tgcgatcggc gtgcggccga gtcgcaggct cgggcagaat ttcctcatcg atcctcgcgt
     5401 tgcggagcgg atcgttgcgc tcgtcgagga tcctcgcgag gcggtgatcg agatcggccc
     5461 cgggctcggc gcgctcacga cgttgcttgc cgccaccggg cgtgagctga ccgcggtcga
     5521 gttggacctg aggttggccg aagcattgga gacgcggctt gccgtgtggc ccgcggcgcg
     5581 ggttgtgcgc ggggacattc tcgagcagcg tgccgaggat ctcgcgagtg gccccgcgac
     5641 ggtcgtcgcg aatcttccct actcgatcac gacgccggcg ctcgagtgga tcgtgacgca
     5701 aggcggacgg gtgaagcgcg cggtgttgat ggtgcagcgt gagtacgcgc aacggctgag
     5761 cgcgaagccg ggtggcaagg agcacgggtc gatcaccgtg ttcctgcagc tccacgcgga
     5821 ggtgagggtg ctgttccgtg tgtcgccggg agcgttccat ccgaggccgg aggtggattc
     5881 cgtcgtgctc gaagttgtcc cgcggcccta cccggggacg acggcggagg agcgcgcctc
     5941 ggtcgagcgt ctggcgcgtg cgggcatggg cacgcggcgg aagacgttgg cgaatgcgct
     6001 ggggcgggcg cttgggatgg agggagcggt ggtgcgggct tggcttgggg cggcgggggt
     6061 ggatccagag cggcgcgggg agacgctgac tgtggatgaa tggatcgcgc tcgcgcgggt
     6121 ggcgcctcgc cgaatgacaa cctgatgggg cgccatactg gggctgggtg tccgagagcc
     6181 ttcccatggg aatgcacgct ggcagtcgtg ctcacttcac tagcgttgtt catgaccttg
     6241 gcctcggccg ttctcgctca ggatacgggc gcgattgttc cggacacaac gttcgtgcct
     6301 cgtactcacc agccgatcag ctactacact tcgtacgacc gaaacatttc acgcgctgcc
     6361 tggatgcaaa ccctttccta tgcccacaac gctagccgcg tagcctttag tctg
//
LOCUS       DASTKC010000245        10900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_448735, whole genome shotgun sequence.
ACCESSION   DASTKC010000245 DASTKC010000000
VERSION     DASTKC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448735"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFR25_10340"
     CDS             <1..912
                     /locus_tag="VFR25_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9941484.1"
                     /translation="KALLEVAGKPILAHILDQVAETDPERVVLVIGPGTQGEQIRQWA
                     ERRPGLRFSFVVQERPLGLGHAVLQAKSEVGTAPVLIVLGDTITVAALRDLVSDGSKV
                     GVREVEDPRRFGVANVEGGRITALVEKPEHPASHLALVGVYYLTNAPLLFRSLEAVER
                     EGKRTNGEIQLTDGLQRMIEEGEVLRPFSVEAWYDCGKTETLLETNRALLDRSAVPVT
                     RPGVVVLPPVAIDESADVAHSVVGPHASIGPGARVHRAVVRNSIISQGATVENVLLDL
                     SVVGENAVVRGTYQRLNVGDSSEVEST"
     gene            909..2105
                     /gene="metK"
                     /locus_tag="VFR25_10345"
     CDS             909..2105
                     /gene="metK"
                     /locus_tag="VFR25_10345"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET9941485.1"
                     /translation="MTLEATQSKPDSRTKPATRHSFLFTSESVTEGHPDKVADQISDA
                     ILDAILKDDPTGRVAAETLVTTGLALVSGEITTKTYVDIPRVVRGTIRDIGYDDPHFG
                     FDWENCAVLTSIDEQSKDIAMGVDRRGAGDQGLMFGYAVRETPELMPAPIQIAHRLTK
                     ALADARRSGKFPEIRPDGKSQVTVEYEAGVPKRIDTVVLSTQHVDMSDADLKRFRERL
                     IQDVIMPVLPKALLHGDPIFHINPTGRFVVGGPRADTGLTGRKIIVDSYGGSAPHGGG
                     AFSGKDPSKVDRSACYAARHVAKNIVGAGLAERCTVQVAYAIGVEEPVSIMVDSHGTG
                     HVPDSRLSELVRKHFDLRPDAIIQSLDLRKPIYKKTAAYGHFGRENEGFRWELLDRVE
                     DLKRDA"
     gene            2116..3378
                     /gene="ahcY"
                     /locus_tag="VFR25_10350"
     CDS             2116..3378
                     /gene="ahcY"
                     /locus_tag="VFR25_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9941486.1"
                     /translation="MNALGHVADPKLAESGVNRIEWAEREMPVLRQIKARFAKEKPLK
                     GRRIAACLHVTTETANLMLTLQAGGAEVTLCASNPLSTQDDAAAALAHRFKIPTYAIK
                     GEDGDTYYKHIQATLDARPEFTMDDGADLVTVLHTKRAEDAARVIAGTEETTTGVIRL
                     RAMAHDGALKYPILAVNDAKTKHFFDNRYGTGQSTLDGILRATNRLIAGSKFVVLGYG
                     WCGRGVATRARGLGAHVLVTEIDPLPALEAVMDGFEVVPAEKAAKVGDFFVTVTGNIN
                     VLRMEHFRAMKDGAIVANSGHFNVELDLDTLKKQAKGVRTVRPFVEEVTLDGGKVVYV
                     LGEGRLINLAAAEGHPASVMDMSFANQALGIEYLLSHSKELKPTVYPIPKALDEEIAK
                     LKLEALGVSIDTLTPEQVAYLASWEQGT"
     gene            3393..5735
                     /locus_tag="VFR25_10355"
     CDS             3393..5735
                     /locus_tag="VFR25_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9941487.1"
                     /translation="MPLLGRVSVRRKYQILGLCLLSIALFLILALTSRDPRDTTADVI
                     GSGSVRNQAGVLGALAASGLSTAFGSLGAWGVPLLLLAWGWNRLRLHAAGELWIKTGL
                     GTAAFLCGSGLIFLLGGENQTWVGAVGGWIGHLAARLLGTIGGELFLGTGLVIVGVVA
                     LELGAASPLRQLVGSWLARLAPQKGRKKKGAAAPAVESAEAEPLATSPRLKRGRPAPA
                     EEEAELERAFGPQPPAPPKESRPRIIGRREAGEPLAIPFPPPAASAAPPAPLERPGTR
                     DDKKRAVEPSPESALGLGPAAAPEAALPSIDLLDQHESRQVAIEESELLDLSRVLERT
                     LADFGVAGKVSEVHPGPVITLFEYEPAPGIKVNQILNRQDDLALALRAQRIRIVAPIP
                     GKAAVGIEIPNRVKALVSIREIIASSAFQKTGDALPFALGKDVAGVPFTASLEKMPHL
                     LIAGATGSGKSVCINTLIMSLLLRRTPSELRFIMIDPKMLELTPYNGIPHLRMPVVTD
                     PKKAAQALRYSVKEMERRYQVLAKHGARNIEAYNRLGLDPSAEDAKLPYLVVVVDELA
                     DLMALLPAEIEEPIGRLAQMARAVGIHLILATQRPSVDVITGMIKANFPSRIAFQVAS
                     RTDSRVILDMNGAESLLGHGDSLYLPAGKPEPYRIHGSYVSSEEIERVVSFLKSQGSA
                     PAIIDDTALEQATSVADSDADDDLYEEAMKLVVLHQQASTSMLQRRLKVGYSRAARLL
                     DLLEERGIVGPSEGAKGREVLLSEHDFEERRSRISEVTEA"
     gene            5732..7129
                     /gene="rimO"
                     /locus_tag="VFR25_10360"
     CDS             5732..7129
                     /gene="rimO"
                     /locus_tag="VFR25_10360"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9941488.1"
                     /translation="MIRPIPLEPARPRDDGTAPAADDARIALVTLGCAKNLVDSEIMA
                     GLLARGGFRTAATPDDADVAVVNTCAFIGPSQKESIDRILELAALKREGRLGGIVVAG
                     CMAQRYEADLIREIPEIDAVVGTGQVDAIARVAHRVRKGGSGIVEVGPAGTAVDLAEH
                     RALSTPRHLAYLRIADGCDFRCTFCIIPTLRGDLRSRPFESVVEEAERLARSGVRELL
                     LIAQDSTSYGEDRYGSSRLPELLRALAQVPGIEWVRIHYTHPKTWSEALIQVFEEVPQ
                     VCRYVDIPLQHVTDRMLRRMRREVDGAGQAGLLRTLRKRLPGIAIRTHFIVGFPGETD
                     EDFEELLNRVKEEEWDHVGCFAYSREDGTPAGRMKEQVPERVKVERRRRLLEAQQAVA
                     ARVWGRWVGREVDVRIDAAGPRPGQYVGRLEQQGYEVDGVTRVRAAAELPMGAKARVR
                     ITRARRYDLEAEVMA"
     gene            7126..7866
                     /locus_tag="VFR25_10365"
     CDS             7126..7866
                     /locus_tag="VFR25_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941489.1"
                     /translation="MMGRRWVGGFLLLGIVASSGTAAAKSPMPPTHSMALDAVADTVA
                     ADLLKGATFPGDRPVVMVTPSPGDTLGLLTQSLMERMKALGVSVRLARGEALAPAMES
                     AVPANGIPLGAAPRDSAAAAASAMSHPADPAAPLRLQVLVEGSGVSYVRRLGSFPFGT
                     KGYERLSGMRASATLFDPQNGDVVWTRSAARSASDVVSKGDVAYAASGSGYLNPPVPR
                     GGGTRWLEPMIVIGVVTGLVVLFYSNRN"
     gene            7901..8629
                     /locus_tag="VFR25_10370"
     CDS             7901..8629
                     /locus_tag="VFR25_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941490.1"
                     /translation="MGVAARQHHVLARDYFERGLYEEAAREIREALAIHGEFPDLHHL
                     LGLALSLSGDREAAVASFRQALHLNPRYVEARLNLAIVLNDLGRYDEALREFQMERPR
                     DPDHENLTPEVRTHLAEAHTQLGDTYRDLGLLVDASQEYRRALRYAPQFLDIKNRLGS
                     VYAEMGLYHDAETVLEEALAQNPRYVDARVTLGLVHWKAGRRARAREAWERCLLDNRD
                     EVRARSYLDFLEREEDRAEEPRAR"
     gene            8626..9390
                     /gene="bamD"
                     /locus_tag="VFR25_10375"
     CDS             8626..9390
                     /gene="bamD"
                     /locus_tag="VFR25_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET9941491.1"
                     /translation="MTFRMSRRVFAVLLLAALVAGCGGTLKEAEVGGVASYERGKGEF
                     DREDWVDAIADLKAYVEQFPGTEKTDDALYYLGESYFNTKDYVLASGQFDRLIRDFPT
                     TPYHPDALYMLARCDDLQSRPAPLDQTETVRAISRYRDFLSLYSDHPKAQDARKRLTM
                     LTDRQAEKAFRNGRLYLRLKQWDAAGHYFEQVIHEYPESRWSLDARVLLADAYVKTGR
                     EAEAADTLRSVLNGTASSGTKREASKRLKKIERSTD"
     gene            9387..9983
                     /gene="nadD"
                     /locus_tag="VFR25_10380"
     CDS             9387..9983
                     /gene="nadD"
                     /locus_tag="VFR25_10380"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET9941492.1"
                     /translation="MRRAERLGVFGGTFDPVHVGHLIMAGEALAKLRLDRMLFVPAAR
                     PAHKRSRTIASAAHRVQMLRLAVRGVARFEVSTLEVERGGVSFTVDTLEHLTKSARDL
                     YFVMGQDSLEDFPTWRDPERIARLARLAVVPRGDGRFPRIRPELRGRVSLLDPPRIGI
                     SSSEIRSRLKRGLSVRWWMPDPALRYAIENGLYGIRRR"
     gene            9964..>10900
                     /locus_tag="VFR25_10385"
     CDS             9964..>10900
                     /locus_tag="VFR25_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9941493.1"
                     /translation="MESGAGKRLLLIDGSALVYRCYYAFIRKPLRNKKGEPTSIPYGF
                     LQTLLPLLASRRPDKVAMVFDTKAKTFRHRVYPEYKAHRPPVPADLTAQLPRSREVIG
                     LLGIPIVEQDGVEADDLIGSLAKEAERAGATTLILTGDKDFFQLVSERTWVLAPQGKT
                     GELQPMDPTAVRERYGVPPEEMVDLLALMGDASDNVPGVAGIGEKTAAQLIQKYHSID
                     ELYRSLDSVERPAIREKLRANEANARLSHTLVTIRADLPLDRHWDELEREPLQARELA
                     SLMTDLEFHTLRRRFAAELDEGGPAEEATPSGDAPT"
BASE COUNT         1762 a         3686 c         3801 g         1651 t
ORIGIN      
        1 aaggctctgc tcgaggtggc ggggaagccg atcctggccc acatcctcga ccaggtcgcg
       61 gaaacggatc cggagcgcgt ggtgctcgtg atcggtccgg gaacgcaggg ggagcagatc
      121 cgccaatggg cggagcggcg ccctggcctt cggttctcgt tcgtcgtcca ggaacggccg
      181 ctcggactgg ggcacgcggt gctccaggcc aagagcgaag tcggcacggc gcccgtcttg
      241 atcgtgctcg gggacacgat cacggtggca gcgctccgcg atctcgtctc cgacggatcg
      301 aaggtgggtg ttcgcgaggt cgaagacccg cgccggttcg gcgtggccaa cgtggaggga
      361 ggtcgcatca cggcgcttgt ggaaaagccg gagcacccag cgtcgcacct cgcgttggtc
      421 ggcgtgtact acctgacgaa cgcgccgctc ctcttccgct cgctcgaggc ggtcgaacgg
      481 gaagggaagc gcacgaatgg ggagattcag ctcacggacg ggctgcaacg aatgatcgag
      541 gagggagagg tgctccgtcc gttttcggtc gaagcctggt acgactgcgg gaagacggag
      601 acgctgctcg agacgaaccg ggcgctcctc gaccgttccg ccgtacccgt gacgaggccc
      661 ggcgtcgtgg tcctgccgcc ggtcgcgatc gacgagagcg cggatgtggc ccactcggtg
      721 gtcgggcccc atgcctcgat cggaccgggg gcgcgcgtgc accgcgcagt cgtccgcaat
      781 tccatcatca gccaaggcgc aaccgtcgag aacgttctgc tcgacctctc ggtcgtgggg
      841 gagaacgcgg tggttcgcgg gacttaccag aggctcaacg tcggcgattc atcggaggtc
      901 gagagcacat gacgctcgaa gcaactcagt ccaagcccga ctcgcgcacc aagcccgcga
      961 cgcggcattc gttcctcttc acctcggagt cggtgaccga gggccatccg gacaaggtcg
     1021 cggaccagat ctcggacgcg atcctggacg ccattctgaa agacgacccg acggggcgcg
     1081 tcgccgccga gaccctggtc acgaccgggc tcgcgctcgt ctcgggggag atcacgacca
     1141 agacctacgt cgacattccg cgcgtggtgc gggggacgat ccgcgacatc gggtacgacg
     1201 atccgcactt cgggttcgac tgggagaact gcgcggtcct cacgtcgatc gacgaacagt
     1261 cgaaggacat cgcgatgggc gtggaccggc ggggcgcggg ggatcagggg ctgatgttcg
     1321 gctatgcggt ccgggagacg ccggagctca tgccggcgcc gatccagatc gcgcaccggc
     1381 tcaccaaggc gctcgccgac gcccggcgct ccgggaagtt cccggagatc cgtcccgacg
     1441 gcaagtcgca ggtcaccgtg gagtacgagg cgggcgtccc gaagcgcatc gacacggtgg
     1501 tgctctcgac gcagcacgtg gacatgagcg acgcggacct gaagcgcttc cgcgagaggc
     1561 tcatccagga cgtcatcatg ccggtcctgc cgaaggcgct cctgcacggc gatccgatct
     1621 tccacatcaa tccgacggga cggttcgtgg tcggaggccc gcgcgccgac accgggctca
     1681 cagggcggaa gatcatcgtc gactcctacg gcggctcggc gccgcacggg ggcggcgcgt
     1741 tcagcggcaa ggatccgtcg aaggtggacc gctccgcctg ctacgcggcg cggcacgtcg
     1801 cgaagaacat cgtgggcgcg ggcctcgcgg agcgctgcac ggtccaggtc gcctacgcga
     1861 tcggcgtcga agagccggtc tccatcatgg tcgactcgca tggcacgggg catgtgcccg
     1921 acagccggct ctccgagctc gtgcgcaagc atttcgatct tcgtccggac gccatcatcc
     1981 agtcgctcga tttgaggaag cccatctaca agaagaccgc cgcctatgga catttcggtc
     2041 gcgagaacga gggcttccgc tgggagctcc tcgaccgcgt ggaggatttg aagcgtgacg
     2101 cctgagaccg ctgtgatgaa cgcgctggga catgtggccg atccgaagct cgccgagtcg
     2161 ggcgtgaatc ggatcgagtg ggccgagcgc gagatgcccg tgctgcgcca gatcaaggcg
     2221 cgcttcgcca aggagaagcc gctcaagggg cgccgcatcg cggcctgtct ccacgtcacg
     2281 acggagacgg cgaacctcat gctcacgctc caggcgggcg gcgccgaggt caccctctgc
     2341 gcgtcgaacc cgctctcgac gcaggacgac gcggcggcgg cgctcgcgca ccgcttcaag
     2401 atcccgacct acgccatcaa gggcgaggac ggggacacgt actacaagca catccaggcc
     2461 accctcgacg cccgtcccga gttcacgatg gacgacggcg ccgatctcgt gaccgttctc
     2521 cacacgaagc gcgcggaaga cgcggcgcgc gtcatcgcgg gcaccgagga gaccacgacg
     2581 ggcgtgatcc ggctccgcgc catggcgcat gacggcgcgc tcaagtaccc gatcctcgcg
     2641 gtgaacgacg cgaagacgaa gcacttcttc gacaatcgct acggcaccgg gcagagcacg
     2701 ctcgacggca tcctgcgcgc gaccaaccgg ctcatcgcgg ggtcgaagtt cgtcgtcctc
     2761 ggctacggct ggtgcgggcg cggcgtcgcg acgcgtgccc gcgggctcgg cgcgcacgtg
     2821 ctcgtcaccg agatcgatcc gctccccgcg ctcgaggcgg tgatggacgg attcgaggtc
     2881 gttccggcgg agaaggcggc gaaggtaggc gacttcttcg tcaccgtgac cggcaacatc
     2941 aacgtgctcc gcatggagca cttccgcgcc atgaaggacg gcgcgatcgt cgcgaactcc
     3001 ggccacttca acgtggagct ggacctcgac acgctcaaga agcaggcgaa gggagtccgc
     3061 accgtgcgtc cgttcgtcga ggaagtcacg ctcgacggcg gaaaggtcgt ctacgtgctg
     3121 ggcgaaggac ggctcatcaa cctggccgcg gccgagggtc atcccgcctc ggtgatggac
     3181 atgagcttcg cgaaccaggc gctcggcatc gagtacctgc tctcgcactc gaaggagctc
     3241 aagccgaccg tctatccgat tccgaaggcg ctggacgagg agatcgcgaa gctcaagctg
     3301 gaggcgctcg gggtgtccat cgacacgctc acgcccgagc aggtcgcgta tctcgcgtcc
     3361 tgggagcagg ggacctaggc gggaggacgc tgatgccgct cctgggccgg gtctcggtcc
     3421 ggcggaagta ccagatcctg gggctctgcc ttctctccat cgcgctgttt ctcatcctgg
     3481 ccctgacgag ccgcgatccg cgggacacga ccgcggacgt gatcgggagc gggtccgtcc
     3541 gcaatcaggc gggcgtgctc ggagctctcg ccgcgtcggg gttgagcacc gcgttcgggt
     3601 cgctcggcgc gtggggcgtc ccgctcctgc tcctcgcgtg gggatggaac cggctcaggc
     3661 tccatgccgc gggcgagctc tggatcaaga ccgggctcgg gaccgcggcg ttcctctgcg
     3721 gctcggggct gatcttcctc ctgggtggtg agaaccagac gtgggtcggt gcagtcggag
     3781 gctggatcgg ccatctcgcc gcacggctcc tcggcacgat cggcggggag ctcttcctcg
     3841 ggacagggct cgtgatcgtg ggcgtggtcg cgctcgagct gggagcggcg tcgccgcttc
     3901 gtcagctcgt cggatcgtgg ctcgcgcgcc tcgcaccgca gaagggtcgg aagaagaaag
     3961 gcgccgccgc tccggccgtg gaatcggcgg aagccgagcc tctcgccaca tcgccgcgac
     4021 tgaaacgagg gaggccggcg cccgccgaag aagaggcgga gctcgagcgc gcgttcggtc
     4081 cccagcctcc ggcaccgccc aaggaatcgc gcccgcgcat catcggacgc agagaagcgg
     4141 gcgagccgct cgcgatccca ttcccgccgc ccgcggcgag cgcggcgccg cctgcgcccc
     4201 tggagcgccc ggggacgcgc gacgacaaga agcgcgcggt ggagccgtcg cccgagagcg
     4261 cgcttggact cggtccggcg gccgctcccg aagcggcgct cccgtcgatc gacctcctcg
     4321 accagcacga gagccgccag gtcgccatcg aggaatcgga gctcctcgat ctctcccgcg
     4381 tgctcgagcg caccctcgcc gatttcggcg tggcggggaa ggtctcggag gtccacccgg
     4441 ggcccgtcat cacgcttttc gagtacgagc cggctcccgg catcaaggtg aaccagatcc
     4501 tgaaccggca ggacgatctc gcgctcgcgc tccgtgcgca gcgaatccgc atcgtcgctc
     4561 cgatcccggg gaaggccgcg gtcgggatcg agatccccaa ccgcgtgaag gcgctcgtct
     4621 ccatccggga gatcatcgct tcatccgcgt tccagaagac gggggacgcg ctcccgttcg
     4681 cgctcggtaa ggacgtcgcg ggcgtgccgt tcacggcgtc cctcgagaag atgccccacc
     4741 tcctgatcgc gggagcgacg ggttccggga agagcgtctg catcaacacg ctcatcatga
     4801 gcctcctgct ccggcgcacg ccgtccgagc tccggttcat catgatcgat ccgaagatgc
     4861 tcgagctcac gccgtacaac gggatcccgc accttcgcat gccggtcgtg accgatccga
     4921 agaaggcggc gcaggcgctc cgctactcgg tgaaggagat ggagcggcgg tatcaggtgc
     4981 tggccaagca cggcgcgcgg aacatcgaag cctacaaccg cctcgggctc gatccttccg
     5041 ccgaggacgc gaagctcccg tatctcgtcg tggtggtgga cgagctcgcc gatctcatgg
     5101 cgctcctgcc ggccgagatc gaggagccga tcgggcgcct cgcgcagatg gcccgcgcgg
     5161 tcggcatcca tctcatcctc gcgacgcagc gtccctcggt cgacgtcatc acggggatga
     5221 tcaaggcgaa cttcccgtca cggatcgcct tccaggtcgc gagccgcacc gactcccgcg
     5281 tgatcctcga catgaacggg gcggagagcc tcctcggcca tggcgacagc ctctatctgc
     5341 cggccggaaa gccggagccc taccgcatcc acggctccta cgtctcgagc gaggagatcg
     5401 agcgcgtggt ctcgttcctg aagagccagg gatcggctcc ggcgatcatc gacgacaccg
     5461 cgctcgagca ggcgacgtcg gtcgccgatt ccgacgcgga cgatgatctg tacgaggagg
     5521 cgatgaagct ggtggtgctg caccagcagg cgtcgacctc gatgctccag cgccggctca
     5581 aggtcggcta ctcccgcgcg gcgcgactgc tcgatctgct cgaagagcgc ggcatcgtcg
     5641 gcccgtccga aggggcgaag gggcgagaag tcctcctgag cgagcacgat ttcgaggaac
     5701 gccggtcccg catctccgag gtgaccgagg cgtgatccgt cccattccgc tcgagcccgc
     5761 tcgtccccgc gacgacggca ccgcaccggc tgccgatgac gcgcgcatcg cgctcgtcac
     5821 gctcggctgc gcgaagaacc tcgtggattc cgagatcatg gcgggactcc tcgcgcgggg
     5881 cggctttcgc accgccgcca cgccggacga cgccgatgtg gcggtggtca acacgtgtgc
     5941 gttcatcggt ccgtcgcaga aggagtcgat cgaccgcatc ctcgagctgg ccgcgctcaa
     6001 gcgcgagggt cgcctcggcg gcatcgtcgt cgcggggtgc atggcgcagc gctacgaggc
     6061 cgatctcatc cgcgagatcc ccgagatcga cgccgtcgtg ggcacgggcc aggtggacgc
     6121 catcgcgcgc gtcgcgcatc gcgtgcgcaa gggcgggagc gggatcgtcg aggtcgggcc
     6181 cgcgggcacc gcggtcgatc tcgccgagca ccgcgcgctc tcgaccccgc gccatctcgc
     6241 gtatctccgg atcgcggacg ggtgcgactt ccgctgcacg ttctgcatca tcccgacgct
     6301 gcggggcgat ctcaggagcc gtccgttcga gtcggtcgtg gaggaggcgg agcgactggc
     6361 tcgatccggc gtgcgggagc tcctcctcat cgcccaggac tcgacgtcct acggcgagga
     6421 tcgctacggc tcgtcgcggc tcccggagct cctccgagcg ctggcccagg ttcccggaat
     6481 cgagtgggtg cggatccact acacccaccc gaagacctgg agcgaagcgc tgatccaggt
     6541 gttcgaagag gtgccccagg tgtgccgcta tgtggatatc ccccttcagc acgtcaccga
     6601 ccgcatgctc cggcggatgc gccgggaggt ggacggggcg ggccaggcgg gactcctccg
     6661 gacgctccgg aagcggctcc cgggcatcgc catccggacc catttcatcg tcgggttccc
     6721 gggagagacg gacgaggact tcgaggagct cttgaaccgg gttaaggaag aggagtggga
     6781 ccacgtcggc tgtttcgcgt attcgcgcga ggacggcacg ccggcgggac gcatgaagga
     6841 gcaggtgccc gagcgcgtga aggtggagcg ccgccggaga ctcctcgaag cgcagcaggc
     6901 cgtggccgcg cgcgtctggg ggcgctgggt cgggcgcgaa gtcgacgtgc gcatcgacgc
     6961 cgcgggtcct cgccccggcc agtacgtcgg ccgcctcgag cagcagggct acgaggtgga
     7021 cggcgtcacg cgcgtgcgcg cggcggcgga gcttcccatg ggagcgaagg cgcgcgtccg
     7081 gatcacgcgc gcgcggcggt acgacctcga ggcggaggta atggcgtgat ggggagacgc
     7141 tgggtgggtg ggttccttct cctgggcatc gtcgcgtctt cgggaacggc ggcggcgaaa
     7201 tcaccgatgc cgcccacgca cagcatggcg ctcgacgcgg tggccgacac ggtggcggcg
     7261 gacctcctca agggggccac gtttcctggg gatcggccgg tggtgatggt cacgccctcg
     7321 ccgggggaca ccctcggact cttgacacag tcgctcatgg aacgcatgaa ggcgctcgga
     7381 gtctcggtgc gtctcgcgcg aggcgaagcc ttggcgccgg cgatggagag cgcggtgccc
     7441 gccaatggaa ttccgctggg agccgcgcct cgggactcag ctgccgccgc cgcttcggcg
     7501 atgagccacc cagccgaccc ggccgcaccg ctccggcttc aagtcctggt cgagggctcc
     7561 ggtgtaagtt atgtgcgccg gctgggttcg ttcccgttcg ggaccaaggg gtacgagcgg
     7621 ctctccggca tgcgggcgag cgcgaccctc ttcgatccac agaatgggga cgtggtctgg
     7681 acgcgctccg cggcccggag cgccagtgac gtggtctcga agggggacgt cgcgtacgcc
     7741 gcgagcgggt cgggatatct caatccgccc gtgccgcggg gaggaggcac ccggtggctc
     7801 gagccgatga tcgtgatcgg tgtggtcacg ggcctcgtgg tgctcttcta ttccaaccgc
     7861 aactgagccg gagggggacg tgattccagg gaggagcgcg atgggagtcg cggcgcgcca
     7921 gcaccatgtg cttgcgcgcg attacttcga gcgcggtctg tacgaggaag ccgcgcgcga
     7981 gatccgggag gccctcgcca tccatgggga gtttccggat ctccaccacc tgctcggtct
     8041 cgccctgagc ttgagcggcg atcgtgaggc cgccgtcgcg tccttccgcc aggcgctgca
     8101 cttgaatccc cgctacgtcg aagcgcgcct gaacctggcc atcgtgctga acgatctcgg
     8161 ccgctacgac gaggcgctcc gcgagttcca gatggagcgg cctcgcgatc cggatcacga
     8221 gaacctgact cccgaagtgc ggacgcatct cgcggaggcg cacactcagc tcggcgacac
     8281 ctaccgggat ctcggactcc tcgtcgacgc ctcgcaggaa taccgccgcg cgctccgcta
     8341 tgcgccgcag ttcctcgaca tcaagaaccg tctcggctcg gtgtacgcgg agatgggtct
     8401 gtaccacgac gcggagacgg tgctcgagga agcgctggcg cagaacccgc gctacgtcga
     8461 cgcgcgcgtc acgctcggcc tggtccactg gaaggccgga cgccgcgccc gtgcgcgcga
     8521 agcatgggag cgctgtctgc tcgacaaccg ggacgaggtc cgggcgcgct cgtacctcga
     8581 cttcctggag cgggaggagg accgggcgga ggagccgcgc gcccgatgac cttccgcatg
     8641 tcccgccgcg tattcgcggt gctcctcctg gccgcgctcg tcgcgggctg cgggggcacg
     8701 ctcaaggaag ctgaggtcgg cggcgtcgcg agctacgagc gcggcaaggg cgagttcgac
     8761 cgagaggact gggtcgacgc gatcgccgat ctcaaggcct acgtcgagca attcccgggc
     8821 accgagaaga cggacgacgc gctctactac ctcggcgagt cgtacttcaa caccaaggac
     8881 tacgtgctcg cgtccggcca gttcgatcgg ctgatccggg acttcccgac gacgccctac
     8941 cacccggacg cactctacat gctcgcacgc tgcgacgatc tgcagtcccg tccggcgccg
     9001 ctcgatcaga ccgaaacggt gcgcgccatc tcgcgctacc gggatttcct ctcgctctat
     9061 tcggaccacc ccaaggccca ggacgcgcgc aagcgcctca ccatgctcac cgatcgccag
     9121 gcggagaagg cattccggaa cgggcggctc tatctccggc tcaagcagtg ggacgccgcg
     9181 gggcactact tcgagcaggt gatccacgaa tacccggaat cgcgctggtc cctcgacgcc
     9241 cgcgtgctcc tcgcggacgc ctacgtgaag acggggcgcg aggcggaggc ggcggacacg
     9301 ctccgctccg tgctgaacgg aaccgcctcg tcggggacga aacgggaggc gtcgaagcgt
     9361 ctcaagaaga tcgagcggag cacggattga ggcgcgccga gcgcctcggc gtgttcggcg
     9421 gcacgttcga tcccgtccac gtcggtcatc tcatcatggc cggggaagcg ctcgccaagc
     9481 tgcgtctcga tcgcatgctc ttcgtcccgg ccgcgcgccc cgcgcacaag cgctcgcgca
     9541 cgatcgcgtc ggccgcgcac cgggtccaga tgctccgcct cgcggtgcgc ggagtggcgc
     9601 gcttcgaggt gtcgacgctc gaagtggagc ggggcggcgt ctccttcacc gtggacacgc
     9661 tcgaacatct caccaagagc gctcgggacc tctacttcgt gatgggacag gacagcctcg
     9721 aagactttcc gacgtggcgc gacccggagc ggatcgcgcg gctggcgcgc ctggccgtgg
     9781 tccctcgcgg ggacggacgg tttccgcgga ttcgaccgga gcttcgagga cgcgtgtcgc
     9841 tcctcgatcc gccccggatc ggaatctcgt cgagcgagat tcgcagccgc ctgaagcgcg
     9901 ggctgtccgt gcgctggtgg atgcccgacc ccgcacttcg ctacgcgatc gagaacggcc
     9961 tctatggaat ccggcgccgg taagcgcctc cttctcatcg acgggtctgc gctcgtctat
    10021 cgctgttact acgccttcat ccggaagccg ctgcggaaca agaaggggga gccgacgagc
    10081 atcccgtacg ggttcctcca gacgctcctg ccgctcctcg cctcgcgccg tcccgacaag
    10141 gtggcgatgg tgttcgacac gaaggcgaag acgttccggc atcgcgtcta tcccgagtac
    10201 aaagcgcacc gtccgccggt gccggccgat ctcacggcgc agctcccgcg ctcgcgcgag
    10261 gtcatcgggc tgctcggcat ccccatcgtc gagcaggacg gcgtcgaagc ggacgatctg
    10321 atcggaagcc tcgcgaagga ggcggagcgc gcgggggcga ccacgttgat cctgaccggc
    10381 gacaaggact tcttccagct ggtctccgag cgcacctggg tcctcgcgcc gcaggggaag
    10441 acgggtgagc tccagccgat ggatcccacg gcggtgcggg agcgctacgg cgtgccgccg
    10501 gaggagatgg tggatctcct cgcgctcatg ggggacgcgt cggacaacgt gcccggcgtg
    10561 gccggcatcg gcgagaagac ggcggcgcag ctcatccaga aataccattc gatcgacgag
    10621 ctctaccgct cgctcgacag cgtggagcgc cccgcgatcc gggagaagct ccgcgcgaac
    10681 gaggccaacg cgcgcttgag ccacacgctc gtcacgatcc gcgccgatct cccgctcgac
    10741 cgccactggg acgagctcga gcgggagcct ctccaggcgc gcgagctcgc ctcgctcatg
    10801 accgatctcg agttccacac gctaaggcgc cgcttcgccg ccgagctgga cgagggaggg
    10861 ccggcggaag aggcgacgcc ttctggcgac gccccgaccg
//
LOCUS       DASTKC010000246        11227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1867683, whole genome shotgun sequence.
ACCESSION   DASTKC010000246 DASTKC010000000
VERSION     DASTKC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867683"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFR25_10390"
     CDS             <1..335
                     /locus_tag="VFR25_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET9941494.1"
                     /translation="PVIMDDTTCMVRAAWITARFFAHESCGQCTPCREGCGWMQRILW
                     KIETGKGEDRDLDILLSVTDQIEGNTICALGDAAAWPTRGFAKKFRDEFLQHIREKRC
                     PLGGGSLH"
     gene            345..1973
                     /locus_tag="VFR25_10395"
     CDS             345..1973
                     /locus_tag="VFR25_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9941495.1"
                     /translation="MATVKLQVDGREVVVEKGTNLIEAGKKAGVFVPSFCYHPGLPVV
                     GVCRICLCEIEGRPKLVAGCATPVEEGMKVITQSAKVREARKAVMEFLLIHHPLDCPM
                     CDKGGECTLQDYTMLMGPSSTRFTFEKQTWPEEDVGGKLILNKNRCILCLRCVNLCKS
                     VAGKDEIAVLSRGEETYIGTVDGRMIENEMAGNIADICPVGALTSKDFRFRARPWEMK
                     PVKSVCTLCSKGCNTQVWYHPRRNEVVRITARENMDVNQWWICDRGRGGFRGVHHPDR
                     LLRPTRRQGDSIGPTTWDHALPAIADSLRSIVERHGASAVGVMASGELTNEECFLARS
                     IFRDGLGIPNVDVAERPQPPVVYPKFTIEGDKNPNTKGARVIGLAPGPKGLGVKDMIR
                     AAAEGSIRAMVFLRGGPLEQFGDAQAVARALGRLECLVVIDILPSPVSERAHWVLPGV
                     SFAEREGTYTNSQGRVQRAPKVFSLRGDTREDWRILADLGRALGVWTHVDPGPQQIFQ
                     RLALSNPAFAGLDYKLIGDLGAPLAASSADVAVG"
     gene            1930..3048
                     /gene="add"
                     /locus_tag="VFR25_10400"
     CDS             1930..3048
                     /gene="add"
                     /locus_tag="VFR25_10400"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9941496.1"
                     /translation="MRRSPPPRRMSPWGDRRLRRDLLLALPKTDLHVHLDGSLRPGTL
                     LELAKEQGVRLAVKTPAELQGFLDELTKGVSLTRYLEAFDLTLRVLQEKEALERAAFE
                     LAEDAHREQVRYMEVRYCPALHTRRKLTMEGTVEAVVRGLERARKRYGIRTGTIICGI
                     RHLSPRLSYRLAELAVAYYGKGVVAFDLAGAEKDYPAKAHRRAFDHVVKHNVNVTVHA
                     GEAFGPASIAQALHYCGAHRIGHGTRLKEDAEILRYVNNHRIPLEMCPTSNVQTGASS
                     SLRAHPFRAYLKLGLRVTLNTDNRLVSSTTMTQELDRVVTALRLEPSDVRHVLLNGFK
                     SAFLPFRERGELIMAAVREIDDVFRKARAGAEPEWDLL"
     gene            3216..4190
                     /locus_tag="VFR25_10405"
     CDS             3216..4190
                     /locus_tag="VFR25_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9941497.1"
                     /translation="MPILQVTTGFWASQALMTAVELRIFTVLHGGPKTAADVAAEVGA
                     EPAALEALLDANCALGFLHRQGDRYRNDDVSNQFLVEGSPGSYVDLVRCMREPLYHLW
                     ENLSETVRTGKASPSAEGKTELATARAYHNGYYATMMRVAEILDIEYSAYRRILDLGS
                     HTGAGALCLARRYPHFQATIQDRALFKPLAEEFIHALKLDDRVSFAPGDPDHAQPDGN
                     YDLVLLPHFLSQRGRDAIPGILANVSASLRKGGTVLVTEFVLEESRAEPKEAALFRLN
                     VLAAHGSAAAGALTRAELVRFLQDAGFTDVDMVGLPMFGITAITACKS"
     gene            4193..4834
                     /locus_tag="VFR25_10410"
     CDS             4193..4834
                     /locus_tag="VFR25_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9941498.1"
                     /translation="MRVERFNVGPFDNNLYLLTAAGSKDAIVVDPSMESGHVLEAIKS
                     RGLNVRRILLTHAHVDHVVMVRAFHEATGAPVWLHVDDRLLYERAGDQAAAMGLAWPG
                     SVPVSHWMTDGEDAGIPGIDARAIHTPGHSPGSITFATPEGLLVGDVLFNGGVGRTDL
                     PGGSWDVLLRTIRERLFPYPPETPVYPGHGALTTIGREMASNPFVGAHALQNP"
     gene            4815..6467
                     /gene="bshC"
                     /locus_tag="VFR25_10415"
     CDS             4815..6467
                     /gene="bshC"
                     /locus_tag="VFR25_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HET9941499.1"
                     /translation="MPSRTHSPVATGLEHLLYPSPLYYDFLTSPERVREFYPVDYRDG
                     AAAARSAASRSYPAERRAAIASVMKRQASELGAASITAPAIAKFEKKDTLAVVAGQQP
                     GLFGGPLYTVYKALTCVHLARRVEEASGRPTVPIFWVASDDHDFDEVRKAWVTDGLGS
                     DPVLLEYEAGGPHASFSRIRIGAAIEPALAKLEESLGAAPEAGLVMARLKDAYAPGRT
                     WAEAFARFMAGVVAPLGMIVFDPADAEAKRLALPVFEREVELGGRSAAVARDRGEALV
                     RRGYHAQIARAGNELNLFWHGGAEREAVRVDEDGAFRAAGGQRWTRQELLDAVRARPE
                     DLSPGVLLRPLVEDYLLPTAAYVGGPSEVAYWAQVFALYPLFEMAPPAIAPRAGATLL
                     EPKVARTLNKFHLSWESLSGDVEVVIREALRAILPEQFRDVFEEERKKWSDGFRKIEE
                     QVLAFDPSLKPAVITAHGRVEREIETLEKKLLHVWKRRQEESVQQIRRARSHLFPHGE
                     LQERVACYAGFASRHGAWLTERLMDALQDPGAHELVSLGGSS"
     gene            6464..7636
                     /gene="bshA"
                     /locus_tag="VFR25_10420"
     CDS             6464..7636
                     /gene="bshA"
                     /locus_tag="VFR25_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HET9941500.1"
                     /translation="MKIGISCYPTHGGSGVVATELGIELARRGHEVHFISYSIPFRLK
                     GYQENVFFHEVQVISYPLFQYPPYTLALSAKMAEIADEHGLDVLHAHYAIPHAVCAYL
                     ARQVAASTKLRIATTLHGTDITLVGSDQSFRTLTRFGIDQSDGVTAVSDYLKRKTVEV
                     FHPQRAIQVIPNFIDTTRYRPRNGDDPCPRERFARKDQRVLVHISNFRPSKRVEDVVR
                     VFAAVRREVPSVLLMVGDGAERTKSRDLAVELGVERHVRYLGQLDAVEDVLRNADLFL
                     LTSENESFGLAALEAMSSGVPVIGTTAEGIPELIREGKTGYMLPVGDVAGMARRAIEL
                     LTDEPLRCSMGKEARRISVENYEASKVIPMYEEFYARITGQTIPARGYLAPPEGLA"
     gene            7787..8794
                     /locus_tag="VFR25_10425"
     CDS             7787..8794
                     /locus_tag="VFR25_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941501.1"
                     /translation="MFWIDSARADTIDGKPRTVSAQIWYPADATTGSAVAPYAAGVDT
                     AGDYGYLLSRVRGHALRSPRFARKVKRAPVLLFSTGRSLSAYDYTSMAEDLASHGYVV
                     VGVNSPGLSRYWRPDGVQVPALRPPSIRVLQKFDSADVVFEPMVRDVSADLRFALNRL
                     EQADRSDPILKDHLDLKRIAMAGHSNGAIAASRACAGDERCRAYFGIEGTQAREVRKG
                     GVTKPFALLISDQSLGFDAENVYRELGTHSASRYTVIIVKGAGHNTFTDLLLVRPYLF
                     QYSIDPKRGAEITRVAIRSFLDTNLLGKDPKLLKSAMTPYSEVEVQTTFTAGSGQPQA
                     H"
     gene            complement(8836..10011)
                     /locus_tag="VFR25_10430"
     CDS             complement(8836..10011)
                     /locus_tag="VFR25_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941502.1"
                     /translation="MKLMSLPLSMFAFACLLVVSCAHKPESTSQLAHLEGQLLDREGN
                     PVGRASLLLSKYDPLFAWPFDEKFAEAKTDSLGRFSLDYKPGEYTLEIMPFSTKGLPS
                     TMIRPFKLSSGPSHKTLRLGGSYLTGRVVLPDGKERRSVSVAAYGESSVVVANYTGHP
                     PYRLLLPTGSYRLVIHDEAYRFPHLDTTLTVPDHDDSLVVILSGRAIGFHLSTKEDQP
                     LSSASILIRTSAGGGYKSTETDSLGNAWALVRPGRYTFEVNPRWGNTRMFQIPWTFAA
                     AIERDTLIAYKLEPVRWSGVARSVKTKDPVPMAEVQFREPGRSGIGHARSDSAGRIEA
                     LMRPGIKYEAASWCLTCYLERFSLALDADTTFDLYLEPGVRPPPKPFTPPHADKPKR"
     gene            10123..11118
                     /locus_tag="VFR25_10435"
     CDS             10123..11118
                     /locus_tag="VFR25_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET9941503.1"
                     /translation="MNHRERIRNTLLGQPTDRPPVSFWRHFYHREGSAEALAEAMLEF
                     QRTYEWDLLKVNPRASYHVEDWGTKTKRPGTGPLDKPKVVESPVKEPGDWAKIRALDP
                     TKGVLGEHLDAEERIAAAVQGDTDWVMTVFNPISIAADLINDDARFVDHLRHHGERVH
                     EALRAITETFAGFVRETMARGASGIFFATTDWANADHIDKELMAEFGRPYDLKVLAEA
                     LGGLLNVIHVCGANAFVDEHLDYPVGVVSWDAHGAGNPKIAALRQRTQKTLLTGIDHE
                     GTFVNGPAAAVQEAAREALQESGGTRFILGPGCAVPAVAPMAHFQAVREVTLACA"
     gene            11109..>11227
                     /locus_tag="VFR25_10440"
     CDS             11109..>11227
                     /locus_tag="VFR25_10440"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET9941504.1"
                     /translation="MRLTVFKSKLHRVRVTEADLNYEGSVTIDADLMDAAQILP"
BASE COUNT         1873 a         3767 c         3752 g         1835 t
ORIGIN      
        1 ccccggtgat catggacgac accacctgca tggtgcgtgc ggcctggatc acggcgcgct
       61 tcttcgcgca cgagtcgtgc ggccagtgca ccccctgccg cgaggggtgc ggctggatgc
      121 agcgcatcct ctggaagatc gagaccggca agggcgagga tcgggatctc gacatcctcc
      181 tctccgtgac ggaccagatc gagggaaaca cgatctgcgc gcttggagac gcggcggcct
      241 ggccgacgcg cggcttcgcg aagaagttcc gggacgagtt cctgcagcac atccgcgaga
      301 agcgctgccc gctcggcggc ggctcactcc actagagggt tcgcatggcg acggtgaagc
      361 tccaggtcga cggccgcgag gtcgtcgtcg agaaggggac caacctcatc gaggccggca
      421 agaaggccgg cgtcttcgtc cccagcttct gctatcaccc cgggctgccg gtcgtcggcg
      481 tctgccgcat ctgcctgtgc gagatcgagg gacgccccaa gctcgtcgcc ggctgcgcga
      541 cgccggtcga agaggggatg aaggtcatca cccagtccgc caaggtgcgc gaggcgcgca
      601 aggcggtcat ggagttcctg ctcatccacc acccgctcga ctgtccgatg tgcgacaagg
      661 gcggggagtg cacacttcaa gattacacca tgctgatggg cccctcctcc acgcgcttca
      721 cgttcgagaa gcagacctgg cccgaggagg acgtgggcgg gaagctcatc ctgaacaaga
      781 accgctgcat cctctgcctc cgctgcgtga acctgtgcaa gtcggtcgcg ggaaaggatg
      841 agatcgcggt cctctcgcgg ggcgaggaga cctacatcgg cacggtcgac ggtcgcatga
      901 tcgagaacga gatggccggg aacatcgcgg acatctgccc ggtcggcgcg ctcacgagca
      961 aggacttccg cttccgtgcg cgcccctggg agatgaagcc ggtgaagtcg gtctgcaccc
     1021 tctgctccaa gggatgcaac acgcaggtct ggtaccaccc gcggcgcaac gaggtcgtcc
     1081 gcatcaccgc gcgcgagaac atggacgtga accagtggtg gatctgcgac cgcggccgcg
     1141 gcgggttccg gggcgtccat catcccgacc gcctgctccg ccccacgcgc cggcaagggg
     1201 actcgatcgg cccgacgacg tgggatcatg cgctcccggc catcgccgac tcgctccgct
     1261 cgatcgtcga gcgccacggc gcgtccgccg tcggcgtcat ggcctcgggc gagctcacga
     1321 acgaggagtg cttcctcgcg cgctcgatct tccgggacgg actcggcatc ccgaacgtgg
     1381 acgtggcgga gcggccgcag ccgcccgtcg tctaccccaa gttcacgatc gaaggggaca
     1441 agaatccgaa cacgaagggg gcccgcgtca tcgggctcgc gcccggcccc aagggcctcg
     1501 gcgtgaagga catgatccgg gccgcggccg aaggctcgat ccgggcgatg gtcttcctgc
     1561 gcggcgggcc gctcgagcag ttcggtgacg cacaggccgt cgcccgcgcg ctcggccgtc
     1621 tcgagtgcct cgtcgtcatc gacatcctgc cctcgcccgt gtcggagcgc gcccactggg
     1681 tgctccccgg cgtcagcttc gccgagcggg aggggaccta caccaactcg cagggacgcg
     1741 tgcagcgagc ccccaaggtc ttcagcctgc gcggcgacac gcgcgaggac tggcgcattc
     1801 tcgccgacct cggccgcgcg ctcggcgtct ggacgcacgt cgatccgggt ccgcagcaga
     1861 tcttccagcg tctcgcgctc tcgaaccccg cgttcgcggg gctcgactac aagctcatcg
     1921 gagatctggg tgcgccgctc gccgcctcct cggcggatgt cgccgtgggg tgatcgccgg
     1981 ctccgccgcg atctcctcct cgcgcttcca aagaccgacc tccacgtcca tctcgacgga
     2041 tccctccgtc ccggcacgct gctcgagctg gcgaaggagc agggagtccg cctcgcggtc
     2101 aagactccgg ccgagctcca gggcttcctc gacgagctga cgaagggcgt cagcctcacc
     2161 cgctacctcg aggccttcga cctcacgctg cgcgtgctcc aggagaagga ggcgctcgag
     2221 cgggccgcgt tcgagctggc cgaggacgcg cacagggagc aggtgcggta catggaagtg
     2281 cgatactgtc cggcgctcca cacgcggcgg aagctcacga tggagggcac cgtcgaggcc
     2341 gtggtccgcg ggctcgagcg ggcgcggaag cgctacggca tccggaccgg gaccatcatc
     2401 tgcggcatcc gccacctgag cccgcggctc tcgtaccggc tcgcggagct ggcggtcgcg
     2461 tactacggaa aaggggtcgt cgcgttcgat ctcgcggggg ccgagaagga ctatccggcg
     2521 aaggcgcatc ggcgcgcgtt cgaccacgtc gtgaagcaca acgtgaacgt caccgtgcac
     2581 gccggggagg cgttcggacc cgcgtcgatc gcgcaggcgc tccactactg cggcgcgcac
     2641 cggatcgggc acggcacgag gctcaaggag gacgcggaga ttctccgcta cgtgaacaac
     2701 caccggatcc cgctcgagat gtgccccacg tccaacgtgc agacgggagc gtcgtcgtcc
     2761 ctccgggcgc acccgttccg ggcgtatctc aagctggggc tccgggtcac gctcaacacg
     2821 gacaaccggc tcgtctcgtc gactacaatg acccaggagc tggaccgggt cgtgactgcc
     2881 ctccgtctcg agccgtccga cgtccggcac gtcctcctga acgggttcaa gtcggcgttc
     2941 ctcccgttcc gcgagagggg ggagctgatc atggccgccg tccgcgagat cgacgacgtg
     3001 ttccggaaag cccgcgccgg cgccgagccg gagtgggatc tgctgtgaaa acggcgcttg
     3061 gcgctgtgaa tcaatctgtt attgcggcat ccaaataggg cggtagcttg agccctctgc
     3121 cggggtggac gtaaactggg gggaatgggg actttacgat gaaccagaca gggaaggccg
     3181 tggggcctcg gccggtgtcg gggcggacga atcccttgcc tattcttcag gtaacgaccg
     3241 ggttctgggc cagccaggct ctcatgaccg cggtcgagct ccgcattttt accgttctcc
     3301 acggggggcc gaagaccgcc gcggatgtgg cggccgaggt cggggcggag ccggcggcgc
     3361 tcgaggcgct cctggacgcg aactgcgccc tgggcttcct ccatcgccag ggcgatcgct
     3421 accggaacga cgacgtctcg aaccagttcc tggtcgaggg atctccgggc tcgtacgtgg
     3481 atctcgttcg ctgcatgcgg gagccgctct accacctctg ggagaacctg agcgaaacgg
     3541 ttcgcaccgg gaaggcttcg ccttcggccg aaggcaagac cgagctcgcc accgcgcgcg
     3601 cctaccacaa cgggtactac gccacgatga tgcgcgtggc ggagattctc gacatcgaat
     3661 acagcgccta ccggaggatc ctcgacctgg ggagccacac cggggcgggc gcgctctgcc
     3721 tggcccggcg ctatccgcac ttccaggcga cgatccagga tcgcgcgctg ttcaagccct
     3781 tggcggagga attcattcac gccctgaagc tcgacgaccg ggtcagcttc gccccgggcg
     3841 atcccgatca cgcgcagccg gacgggaact acgatctcgt cctcctgccg cacttcctct
     3901 cacagcgcgg ccgcgacgcg atcccgggca tcctggcgaa tgtctccgct tccctccgca
     3961 aggggggcac ggtgctcgtc accgagttcg tgctcgagga gtcgcgcgcg gagcccaagg
     4021 aggcggcgct cttccggctg aacgtcctcg cggcccatgg atcggcggcg gcgggtgcgc
     4081 tcacgcgcgc cgagctcgtc cggttcctcc aggacgcggg cttcacggac gtggacatgg
     4141 tcggactgcc gatgttcggc atcaccgcca tcaccgcgtg taagagctga ccatgcgcgt
     4201 cgagcggttc aacgtcgggc cgttcgacaa caatctctac ctgctcacgg cggccggatc
     4261 caaggacgcg atcgtggtcg atccgtcgat ggagagcggg cacgttctcg aagcgatcaa
     4321 gtcccgtggc ctgaacgtcc gccgcatcct gctcacgcac gcgcacgtcg accacgtggt
     4381 catggtgcgc gcctttcacg aagcgaccgg agcgccggtc tggctccacg tcgacgaccg
     4441 gctcctctac gagcgcgcgg gcgatcaggc cgcggcgatg ggtcttgcat ggcccggcag
     4501 cgtccccgtg tcccactgga tgacggacgg cgaggatgcc ggcatcccgg gcatcgacgc
     4561 gcgcgccatc cacacgccgg ggcactctcc gggcagcatc acgttcgcca cgccggaagg
     4621 gctcctggtc ggtgacgttc tcttcaacgg aggcgtcggc cggaccgacc ttccgggtgg
     4681 aagctgggac gtgctcctgc gaacgatccg cgagcggctc tttccttatc cccccgagac
     4741 cccggtctac ccggggcacg gggcgctcac gacgatcggc cgcgagatgg cgtcgaatcc
     4801 cttcgtgggc gcccatgccc tccagaaccc atagtcccgt cgcgacgggg ctcgagcacc
     4861 tcctctaccc gagcccgctc tactacgact ttctgacctc gcccgagcgg gtgcgcgagt
     4921 tctaccccgt cgactaccgg gacggagccg ccgccgcgag gagcgccgcg tcgcgttcct
     4981 atcccgccga gcgtcgcgcg gccatcgcgt cggtgatgaa gcggcaggcg tccgagctcg
     5041 gggcggcctc catcacggcc cccgccatcg ccaagttcga gaagaaggac acgctcgccg
     5101 tggttgcggg gcaacagccc ggtttgttcg ggggcccgct ctacaccgtc tacaaagcgc
     5161 tcacgtgcgt gcacctcgcg cgccgggtgg aagaggcgtc gggacggccc accgtgccga
     5221 tcttctgggt ggcctccgac gatcacgact tcgacgaggt gcgaaaagca tgggtgacgg
     5281 acggactcgg ctccgacccc gtgctgctcg aatacgaggc gggcgggccg cacgcgtcct
     5341 tctcgaggat tcgcatcggc gcggcgatcg agcctgcgct cgcgaagctc gaggagtcgc
     5401 ttggcgcggc tccggaggca ggcctggtca tggcccggct caaggacgcg tatgcgcccg
     5461 gccgcacgtg ggcggaggcg ttcgcccggt tcatggccgg ggtcgtcgct ccgctcggca
     5521 tgatcgtctt cgatccggcg gacgccgagg ccaagcggct cgcgctcccg gtgttcgagc
     5581 gcgaggtgga gctgggcgga cgctccgccg ccgtcgcgcg cgaccggggc gaggcgctcg
     5641 tgcgccgcgg ctatcacgcg cagatcgccc gcgcgggaaa cgagctcaac ctcttctggc
     5701 atggcggcgc cgagcgcgaa gcggtgcgcg tcgacgagga cggcgcgttc cgcgccgcgg
     5761 gcgggcagcg ctggacgcgc caggagctcc tcgacgcggt gcgcgcgagg cccgaggact
     5821 tgagccccgg cgtgctgctc cggccgctgg tcgaggacta cctgcttccg accgccgcct
     5881 acgtcggcgg gccctccgag gtggcctact gggcacaggt gttcgcgctc tatccgctct
     5941 tcgagatggc gccgcccgcg atcgcgccgc gcgccggagc gacgctcctc gagcccaagg
     6001 tggcgcgcac gctgaacaag ttccacctct cgtgggagtc cctgtcggga gacgtcgagg
     6061 tggtgatccg cgaagccctt cgcgccatcc tccccgagca gttccgggac gtgttcgagg
     6121 aggagcggaa gaagtggtcc gacggcttcc gcaagatcga ggagcaggtg ctcgcgttcg
     6181 atccgtcgct caagccggcg gtgatcaccg cgcacggacg cgtggagcgc gagatcgaga
     6241 ccctcgagaa gaagctcctc cacgtctgga agcgccggca ggaggagtcg gtccagcaaa
     6301 tccgccgcgc gcggtcacat ctgttcccgc acggtgagct ccaagaacga gtcgcctgct
     6361 acgcgggctt tgcgtcgcgg cacggcgcgt ggctgacgga gcggctcatg gacgcgctcc
     6421 aggaccccgg cgcgcacgag ctcgtgtccc tgggaggttc ctcgtgaaga tcggcatcag
     6481 ctgctatccc acccacggcg gatcgggcgt ggtggcgacc gagctcggga tcgagctggc
     6541 gcgccgcgga cacgaggtcc acttcatttc gtactcgatc ccgttccgtt tgaagggtta
     6601 ccaggagaac gtgttcttcc acgaggtgca ggtcatctcg tatccgctct tccagtaccc
     6661 gccgtacacg ctggcgctgt cggcgaagat ggcggagatc gcggacgagc acggactgga
     6721 cgtgctccac gcgcactacg cgattccgca cgccgtctgc gcctatctcg cgcggcaggt
     6781 ggcggcgtcc acgaagctcc ggatcgcgac cacgctccat ggaaccgaca tcacgctcgt
     6841 cggctcggac cagtcgttcc gcacgctgac ccggttcggc atcgaccaga gcgacggcgt
     6901 gacggcggtc tcggattatc tgaagcgaaa aacggtcgag gtcttccacc cgcagcgcgc
     6961 catccaggtg atcccgaact tcatcgacac cacgcgctac cgtccgcgca acggcgacga
     7021 tccgtgtccc agggagcggt tcgcccgcaa ggaccagcgc gtgctcgtcc acatctcgaa
     7081 cttccgcccg agcaagcgcg tggaggacgt ggtgcgcgtc ttcgccgccg tgcgccgcga
     7141 ggtgccgagc gtgctcttga tggtggggga tggcgcggag cgcacgaaga gccgcgacct
     7201 cgcggtggaa ttgggtgtgg agcgtcacgt ccgctatctc ggccagctcg acgcggtcga
     7261 ggacgtgctc cggaacgcgg atctcttcct cctgacgagc gagaacgaga gcttcggcct
     7321 cgcggcgctc gaggccatgt cctcgggcgt gccggtcatc ggtacgaccg ccgagggcat
     7381 tcccgagctg atccgcgaag ggaagaccgg ctacatgctc ccggtcggcg acgtggccgg
     7441 catggcgcgc cgggcgatcg agctcctcac cgacgagccg ctccgctgct cgatggggaa
     7501 ggaagcgcgc cggatctcgg tcgagaacta cgaggcgagc aaggtcatcc cgatgtacga
     7561 ggagttctac gcccgcatca ccggccagac gatcccggct cgggggtacc tggcgccgcc
     7621 ggaaggattg gcctagggcg tgctcgtggc ttgctggagc accctgctgc ttgctgtcgt
     7681 gttggtcgtg gcatcgccct gtcccgccca gatcccccat tcggccgcgg attcgctcat
     7741 ccagctgccg gcacccacag ggacgcaagc cgtcggtacc accaccatgt tctggatcga
     7801 ctcggcgagg gccgacacga tcgatggcaa gccgcgcaca gtctccgctc agatctggta
     7861 tccggccgat gcgaccactg gatcggcggt cgcgccgtac gccgctggcg tcgacaccgc
     7921 aggcgactac ggttacctcc tgtccagggt tcgcggacac gccctgcgat ctccgcgctt
     7981 cgcacggaag gtcaagcgcg cccccgttct cctgttctcg accgggcgct ccctgtccgc
     8041 gtacgactac acgtcgatgg ccgaggacct ggcgagccac ggatacgtgg tcgtcggggt
     8101 caacagcccc ggcctgtcac gctactggag gcctgacgga gtgcaggttc cggctctgcg
     8161 acccccatcg atccgggttc tgcagaagtt cgactccgcg gacgtcgtct tcgagccgat
     8221 ggttcgcgac gtgtccgcgg acctccggtt cgcgctcaac aggctggagc aggccgatcg
     8281 ctcggacccg attctcaagg accacctcga tctgaagcgg atcgccatgg cgggccactc
     8341 gaacggggcc atcgctgcgt cgcgggcgtg cgccggcgac gagcggtgcc gcgcatactt
     8401 cggcatcgag gggacccagg cgcgggaagt ccgaaagggc ggagtcacga agcccttcgc
     8461 gctgctcatc agcgaccagt cactgggatt cgacgccgag aacgtctacc gggagctcgg
     8521 cacccactcc gcctcgcgct acacggtgat catcgtgaag ggggcgggac acaacacgtt
     8581 cacggaccta ttgctggtgc gcccgtacct gttccagtac tccatcgacc cgaagcgcgg
     8641 cgcggagatc acgcgtgtcg cgattcggtc cttcctggac acgaatctgc tgggcaagga
     8701 tccgaaactt ctgaaatccg ccatgacgcc atattcagag gtggaggtgc agacgacctt
     8761 cacggccggg tccggccagc cccaggcaca ctgacttcct ggggtatttt gctggcctac
     8821 accgttagtg gacgatcagc gcttgggctt gtctgcgtgt ggcggagtga aaggcttagg
     8881 gggagggcgt acgccgggtt ccaagtacaa atcaaatgtc gtatcggcgt cgagcgcgag
     8941 cgaaaaccgc tctagatagc aagtgaggca ccagcttgct gcctcgtact tgatccccgg
     9001 acgcatcaat gcctcgatcc ttcccgcgct atccgaacgc gcgtggccga tccccgacct
     9061 gcccggctct cgaaactgga cctcagccat cggaacagga tcctttgttt tgacggatcg
     9121 ggccaccccg gaccatcgca ctggctcgag cttgtaggct atgagcgtat cgcgctcaat
     9181 tgccgccgca aaggtccacg ggatctggaa catccgtgtg ttaccccatc ggggatttac
     9241 ctcaaacgtg tagcggccgg ggcgtaccag agcccatgca ttcccaagag aatctgtctc
     9301 cgtggacttg tatccgcctc cggcggacgt acggatgagg atgctagcgc ttgataatgg
     9361 ctgatcctcc tttgtgctca gatgaaaacc aatggcgcgg ccactcagga tcacaaccag
     9421 cgagtcgtcg tggtccggaa cggtcaatgt cgtatcgagg tgcgggaatc gataagcctc
     9481 gtcatggatg accaagcgat acgaccccgt gggaagtagg aggcggtacg gtgggtggcc
     9541 agtgtagttt gcgacgacca cggagctctc gccataggct gcgaccgaga ccgaacgccg
     9601 ttccttcccg tcaggtaaga cgacacgccc ggtcaaatag ctgcctccga gccggagggt
     9661 tttgtgtgaa ggacccgaag agagcttgaa gggtctgatc attgtcgaag gtaggccctt
     9721 ggtgctgaag ggcatgatct ccagcgtgta ctccccgggc ttgtaatcga gactgaatct
     9781 cccaagagag tcggttttcg cttccgcaaa cttttcatca aagggccaag caaacaaggg
     9841 gtcgtatttg gacaagagaa ggctcgccct tccgaccgga ttaccctccc gatcgagcag
     9901 ttgcccttcg agatgggcca gctgggacgt gctttcgggt ttgtgggcgc aggagacgac
     9961 caacaggcac gcgaacgcga acattgagag gggcaggctc atcagcttca ctacgagggt
    10021 cccccggctg cggggcttgt ggcggagagg ggaaaacggt acgattccgg aagcctacat
    10081 cctttcgagt tctcccaacc acaacggatt gcggtatttc ccatgaacca tcgtgaacgc
    10141 atacggaaca cccttctcgg ccagccgacc gaccggccgc cggtcagctt ttggcggcat
    10201 ttctatcacc gggaggggtc ggcggaggcc ctggcggagg ccatgctcga gttccagcgg
    10261 acctacgagt gggacctcct caaggtgaac ccacgtgcgt cctaccacgt cgaagactgg
    10321 ggaacaaaga ccaagcgccc cgggaccggg ccgctcgaca agccgaaggt ggtcgagagt
    10381 cccgtgaagg agccgggcga ctgggcgaag atccgcgcgc tcgatccgac caagggcgtc
    10441 cttggcgagc atctcgatgc cgaggagcgc atcgccgcgg ccgttcaggg ggacaccgac
    10501 tgggtgatga ccgtgttcaa tccgatctcg atcgcggccg atctcatcaa cgatgacgcg
    10561 cgcttcgtgg accacctccg ccatcacggc gagcgggtgc acgaggcgct gcgcgcgatc
    10621 accgagacct ttgccggatt cgtgcgcgag accatggcgc gcggcgcgtc ggggatcttt
    10681 ttcgccacga ccgattgggc caacgcggac cacatcgaca aggagctcat ggccgagttc
    10741 ggccggccgt acgatctcaa ggtgctcgcg gaggctttgg gcggcctctt gaacgtgatc
    10801 catgtctgcg gcgccaacgc cttcgtcgac gagcacctcg actatccggt cggggtcgtg
    10861 tcgtgggacg cgcacggggc agggaatccg aagatcgccg cgctgcgtca gcggacgcag
    10921 aagacgctcc tcaccgggat cgatcacgaa gggacgttcg tgaacgggcc cgcggccgcc
    10981 gtccaggagg cggccaggga ggcgctccag gaatcgggcg gaacccgctt catcctcggc
    11041 ccgggctgcg cggttcccgc cgtcgcgccc atggcccatt tccaggccgt ccgggaggta
    11101 acgctcgcgt gcgcttgacc gtcttcaaat cgaagctcca tcgcgtccgt gtcaccgagg
    11161 ccgatctcaa ctacgaggga tcggtcacga tcgacgccga tctcatggac gccgcccaga
    11221 tcctgcc
//
LOCUS       DASTKC010000247        14783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1868076, whole genome shotgun sequence.
ACCESSION   DASTKC010000247 DASTKC010000000
VERSION     DASTKC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14783
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868076"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2692)
                     /locus_tag="VFR25_10445"
     CDS             complement(<1..2692)
                     /locus_tag="VFR25_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941505.1"
                     /translation="MLASCCIPVGAWAAPGSGTASIVPTAPVLAGASGAWTIRYVAAE
                     DFAFPQGGWLYVQVPAGWTAPQIAQPAQPGYVQITDMSLVNQILVAGNTIELRLGGAS
                     TSRFETGDTLTLVYGAGTPQAQVQPTGPDVAVFWVSSDPAGSSPAPIGASPVLEVDGP
                     LDHVRVEDAAGAEVAALALDADQDTTTLYLRGYDVADHPLGLVAGTWSVTGGIGSVPA
                     GPASSAVLTLTTVGSGRVVSANGAITDSTGVITVTHGAPSSIDATFPPGGSAGTGLLA
                     TVHARDADGNTVTTGPISGASVSVHAFTGASGPAPADPDFITSQVALTAGAWSGMLTP
                     RRAGAYWVTAFDAGSGFESAPRAPLSVSAGSADHIVLVPGTLALVAGASDTVSVLAYD
                     AFGNRASLATAETLTLWTDRPGGHFAAVGGGAIFEIAVPAGADSARFTFTDVTSGGGT
                     GHIRAIDANGSGASLGTAEASVTTIPGVPFGNVALSALPATLVANGVDSTSITSGAVR
                     DAYGNVVAAGEKVTVSGSGLTPLGDQDAGTPGVQWTTGVSGTLQGWARAGTAAGAGSL
                     SLVSVQGSATGSIPVPLASGVPAGAIALLASPDSVAADSVALRGVTASGLRDAFGNTV
                     VDGERYTVATTLGSIVATDRDSTTAGVQVEASGGSIAFSLLGGVTLGTANVTATSVRG
                     SASGSIPIRIVPGPVSAARSSVAAVSPATVGPAGSTVTVTLRDARDHTLPLVPASSIV
                     LSWSGPPGVSFSALGAGTGASGSIDFRAFTTLTMVGAVHATVSGVPLLMAPTISFMAG
                     SPDTLVVTGPAGPLLAGSSQALDVRARDAYGNDTPAAAGFFVRPTVLTGAAQVPDSAA
                     VSGGAGTVPFTPVAAAPLSIRIADDFGHATTYGPVTVTSGP"
     gene            complement(3049..4023)
                     /locus_tag="VFR25_10450"
     CDS             complement(3049..4023)
                     /locus_tag="VFR25_10450"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9941506.1"
                     /translation="MFAEDLLQDLRKEGFTPTSVARYLRRILARIVGTLAERADLVRS
                     VAGTSFVLFAIQFGAALLLSWAFGRRVGVTYIIASSVTLLIASFWILAHIGLAGTPEV
                     RSLRRIPLPVALTLLRLAAVPAIVLLAREGRWSVVVWLFAASAFTDVLDGVVARALHM
                     ESIIGAVLDPAIDVFFNATVFIALTVFGALPWWVTALMLARYGILILGTCYLYLFHGP
                     VKIQPTAFGKLTGLVTSVLVGLLLLGLAYWSDAMRSRLKEVFDVGLGVLAGATIIQVL
                     FIGLANKRALDQDRLLEQILPPAPPQGLEPGSRAGSGKVVGDVRWPRR"
     gene            complement(4026..5222)
                     /locus_tag="VFR25_10455"
     CDS             complement(4026..5222)
                     /locus_tag="VFR25_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9941507.1"
                     /translation="MRTSPPNPRILPVALLLALAFGAVGYTPPPLFRRDVNLQPAETC
                     KTCHLSITESWQNSAHSKAERSQNLLFGRMYFQSLKTTRGGTFLNCGPCHEATTYVNQ
                     DFNFVRDVSHEGVTCVFCHMVSAPGPSTGNPPFELDLNHYYGSIRNPVPTNAHGSEVS
                     TYLRTSGFCGGCHAYKNQNGVTISDTYAEWKASKYAKQGVSCQTCHMPGQPGRVSYLG
                     PSRPKVPDHSFSAEVAEKAQPGPAATLVLRGTKTTSGDSLSLSAVVTNTGWGHSLPTG
                     NDQNMAIVRIRVLTADGAIVWENDPFTEWANSVFGVILQDELGNWPVETWVATKVLQN
                     RRIKAGASATARYRVPVGDHKGPYKIEAQLLFRRARPNTIEAYALDETTFGTERVLAE
                     ASLRVP"
     gene            complement(5236..5985)
                     /locus_tag="VFR25_10460"
     CDS             complement(5236..5985)
                     /locus_tag="VFR25_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020597206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9941508.1"
                     /translation="MVRVESPSPPVFTVDCVIPALNEASSIGRVLEELPRPLVRRAVV
                     SDNGSSDGTADVARAHGALVVSEPERGYGAACLRGIAALASDPPDIVLFLDGDLSDDP
                     SEADLLVEPIRSGRADLVIGSRTRGVREPGALTPQARFGNWLATRLIRSLYGVRYTDL
                     GPFRAIRWDSLRRLGMQDRAFGWTVEMQVKAARRGVRAEETPVRYRRRIGRSKISGTV
                     SGSLRAGVAILGTIAAEAFQGGRLEPPGSTL"
     gene            complement(5991..8867)
                     /locus_tag="VFR25_10465"
     CDS             complement(5991..8867)
                     /locus_tag="VFR25_10465"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9941509.1"
                     /translation="MSAQETATKAAAKASPRPADQPKHVYSFGAGSAEGNARMRDLLG
                     GKGAGLAEMTNAGIPVPPGYTITTAACRQFFEAGGRLLESTVQEEKDALRKLERYLGR
                     RLGDPENPLLVSVRSGAPVSMPGMMDTVLNLGLNDQTVPGLAQRSGHPRFAHDSYRRF
                     IQMYSDVVLGVPRALLERELTREKARAKVELDVDLTAERLEALSRRFLDIVKRETGRD
                     FPQDPRVQLAGARDAVFRSWNTERAVHYRRIHGIPEGLGTAVTVQAMVFGNLGLSSAT
                     GVGFTRDPGTGERRFYGEYLRNAQGEDVVAGIRTPRPIEEMERELPEAYGQLREITSR
                     LERHYRDVQDFEFTVEEGRLFMLQTRRAARTGIAALKIAVEMVEEGLLTKREGIERVE
                     PAHLDQVLHPVLDPAARKKHDKVATGLPASPGAAAGAIVFTSDEAVRRGASERVILVR
                     AETSPDDIAGMHAAQGILTATGGMTSHAAVVARGMGKCCVVGCSALQIHEEERVLVIG
                     GKKLKEGAFLSLDGSTGEVLVGDVPTTDSEVVRVLTGELPAAKAPLYQCFERLMAWCD
                     EERRLRVRANADVPRDAKIARHLGAEGIGLCRTEHMFFAPDRIPHVVAMILNAQRARD
                     AKVRIARIEAELAEAPRSERARLNAELLRERRESKPTIQAYEGALAKLLPLQRADFKG
                     LFLAMEGCPVTIRTLDPPLHEFLPKRAELEAQLREMGKKGTPARRKEIERTLARVEEL
                     HEFNPMLGFRGCRLGILHPEITRMQARAVFEAAVQVLKAGKRVIPEIMIPLVGDPEEL
                     RRQRAEVEAVAEEVMKKAGVTIPYTIGTMIEVPRAAVLADEIAQHADFFSFGTNDLTQ
                     MTFGYSRDDSGRFLPEYASSGILPGDPFVSIDVKGVGTLVQRAAFAGRRAKPGLKIGI
                     CGEHGGDAASIDFFENVGLDYVSCSPFRIPLARLAAARAALKRK"
     gene            complement(8896..9963)
                     /locus_tag="VFR25_10470"
     CDS             complement(8896..9963)
                     /locus_tag="VFR25_10470"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET9941510.1"
                     /translation="MAVTQRQARGVDLAAADARATPLVHVLRDERVESIHRGHVAVVD
                     ARGRLLAFTGEPKALVFPRSAFKPFQALPLVESGAFAKSGLASDALALIAGSHSGTDR
                     HAALARTILRAAGVDESMLQCGVHPPYDEATAERLRRAHEEPTPVRHNCSGKHAGMML
                     FARYLGAPVETYADPSHPVQQHIFDRFASLLGEPWTDPEPAIDGCSAPTPRMPLDTLA
                     RAFALLASGQDSGGAPLPALAAIRDAMQEHPELVAGPGRLDALLMRATPSAVAKAGAE
                     AVHAIGLVDIGVGIAVKVEDGSRRALGPAVVSVLEALGVLGDAERRALAQHAGEPLRN
                     AAGLEVGSIRGVARLEGGGLR"
     gene            complement(9995..11542)
                     /locus_tag="VFR25_10475"
     CDS             complement(9995..11542)
                     /locus_tag="VFR25_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9941511.1"
                     /translation="MNPQTASPSAEPAERTRTADPAARSYLNLIGGAWVPARTGRTFE
                     NRNPARPSDLIGTFPESGKEDVEAAVQAAQRAFKSWRLVPAPKRAEIIYRAAEILREK
                     KEAFARAMTREMGKVLAETRGDVQEAIDMAYYIAAEGRRLFGVTAPSELPNKFAMSIR
                     QPMGVCGLITPWNFPMAIPSWKIFPALVTGNTVVIKPATDTPASAHHLVEVLVEAGVP
                     EGVLNLVHGGGSAVGVPLSESPGVAMVSFTGSSEVGKTIAERTASSLKRLSLELGGKN
                     AQIVLADADLDLAVEGVLWGAFGTTGQRCTATSRLIVEKPVHDAMLARLIERAQKLKL
                     GDGLEPGTDVGPVVNRGQKERVHSYVEIGKAEGAKLVAGGSPAEDGALAQGHFYKPTI
                     FDQVTPSMRIAVEEIFGPVLSVITVSSFEEAIQVLNNTPYGLSSSIYTADVNRAMRAI
                     RDIEAGITYVNGPTIGAEVQLPFGGVKETGNGHREGGPTVIDAFTEWKSVYIDYSGKL
                     QKAQIDS"
     gene            complement(11583..12542)
                     /locus_tag="VFR25_10480"
     CDS             complement(11583..12542)
                     /locus_tag="VFR25_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941512.1"
                     /translation="MFRNPLFRLGFVMSLCTVLLAHAAPAPGQTLTSPTGVKGYYLQT
                     HTANQVNNQTGTFVWILWSSSVEYDSVCTPQCVPVRNGYRVRRTIEGISPSRLELVGQ
                     WMARDRVGPVCLAQQAPCDVQNFVFTGTGIFFRGFYNNKLPDGSYILDYPPGNPADVD
                     TTARIFVDLAPLAGFRHEYAVTSIDTTTSVNADFAESPIDSSQLVYLTPATPPADNME
                     HIAVVPNPYKGSAEWDLGPNQREIHFINVPAGATIRIYTSNGELLRVLTQNPNSSPGG
                     VTGEVPWDLKNDDGRTVVSGIYLYTVHPTDGRTPKKGHFVIIK"
     gene            12964..>14783
                     /locus_tag="VFR25_10485"
     CDS             12964..>14783
                     /locus_tag="VFR25_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941513.1"
                     /translation="MKKLLGVIGILALAAAMLPAQGHADLRGSFAPITIASGFSQGCE
                     TASHRPDDGTGTMLFPVDGGRLEPEWITFTARSLVVGHENVRSIYLHLNAQQSGYFAV
                     IKTDPLVGSFGHDDISYFDPAWSYDGRFLAYIKSVGNTASGLFVQEYFVSDDFEAVYG
                     PGMGADSPVGAEWLVTNAGDPRHPAWHPSSYVLAYDTSVGTSRDIYTQTIDVGPGHID
                     GPPVRVTFDNAKAEQDPAWSPNGTDIAYTTNKFGPLQIEIVNTSLPPADPNYTHLAEP
                     NFAFVTHNNPSYDPTGAQLLYDAPGGENVNNVVSIWRLDLATKAKCEMQLDNRADADP
                     SWSPFVQHTRAPESLPYSYFVMTSQGGNFGVNIWRANLINSCLTPLPMAVTITPNVIN
                     WKSENKGNNDGVPFVTKMKYPAETKALGYVCRSANTGGEGTRLRSTIFNSPTLLNLSC
                     PTNADLHPQGLTGVPNTECFDTADTLSVSPLTLERTITCWFDRRTILDQITALGLVDK
                     QVPMEMTAYSNLTGRKFQGFAYIKVNKVSGASSNVAMISNSPNPFNPVTQIKFAVSKA
                     GTYSLRIYNVQGALVKSLASQRYDIGTHEATWDGRTNTGG"
BASE COUNT         2374 a         5009 c         5129 g         2271 t
ORIGIN      
        1 cagggccgga cgtcacggtg acggggccat aggtcgtggc gtggccgaaa tcgtccgcga
       61 tgcggatcga gagcggagcc gccgcgaccg gcgtgaacgg aaccgtcccc gcgccccccg
      121 agaccgccgc cgaatcggga acctgggcgg cgcccgtgag gacggtggga cgcacgaaga
      181 agccggccgc ggcgggagta tcgttcccgt aggcgtcacg cgcgcgcacg tcgagcgcct
      241 gggacgatcc cgccaggagc ggaccggctg ggccagtcac cacgagcgtg tcgggtgagc
      301 cggccatgaa cgagatcgtg ggagccatga gaagcggcac gccggagacg gtcgcgtgca
      361 cggctcccac catcgtgaga gtcgtgaagg cgcggaaatc gatcgagccc gaggcgccgg
      421 tgcccgcgcc aagcgcggag aacgagaccc ccggcgggcc gctccacgag agcacgatcg
      481 acgatgcagg cacgagcgga agggtgtgat cgcgcgcgtc gcgcagcgtc accgtgaccg
      541 tgctgccagc cgggccgacc gtcgcgggcg acaccgccgc gaccgaagag cgcgccgcgc
      601 tcacgggccc ggggacgatc cggatcggga tcgagcccga cgcgctcccg cgcacggacg
      661 tcgcggtgac gttcgccgtg ccgagcgtga cgccccccag gagcgagaag gcgatcgatc
      721 cccccgacgc ttccacctgg acgcccgcgg tcgtcgagtc gcgatccgtg gcgacgatcg
      781 atccgagcgt cgtggccacg gtgtagcgct cgccatcgac caccgtgttt ccgaatgcgt
      841 ccctgagccc cgatgcggtg acgccgcgga gggccacgct gtccgccgcc accgaatccg
      901 gcgacgccag gagcgcgatc gccccggcgg ggacgcccga tgcgagcgga accgggatcg
      961 agccggtcgc agagccctgg accgagacga gggacagcga tcccgcgccg gcggccgtgc
     1021 ccgcgcgcgc ccagccctgg agcgttccgg agactccggt cgtccactgc acgccgggcg
     1081 tgcccgcgtc ctgatcgccg agcggcgtca atcccgagcc gctcacggtg accttctcgc
     1141 ccgctgcgac gacgtttcca tacgcgtcgc gaacggcgcc ggacgtgatc gaagtggaat
     1201 ccactccgtt cgcgacgagc gtcgcgggga gcgccgacag cgcgacgttt ccgaacggca
     1261 cgccgggaat cgtcgtgacc gaagcctcgg ccgtcccgag cgatgctccc gatccgttcg
     1321 cgtcgatcgc ccggatgtgc cccgttccgc cgccggacgt gacatccgtg aacgtgaacc
     1381 gcgccgagtc cgcgcctgcg ggcacggcga tctcgaagat cgcgccgcct ccgaccgcgg
     1441 cgaaatggcc gcccggacgg tcggtccaga gcgtgagcgt ctcggccgtc gcgagggacg
     1501 cgcgatttcc gaacgcgtcg tacgcgagga ccgacaccgt gtccgacgcg cccgcgacga
     1561 gcgcgagcgt gcccggcacg aggacgatgt gatcggcgga gcccgcggag accgagagag
     1621 gcgccctcgg agccgattcg aagccgctcc ccgcatcgaa cgccgtgacc cagtacgcgc
     1681 ccgcgcggcg cggagtgagc atcccgctcc acgcgcctgc ggtgagcgcg acctggctcg
     1741 tgatgaagtc gggatcggcc ggggcggggc ccgaggcgcc cgtgaaggcg tggacggaga
     1801 cgctcgctcc cgagatcggc cctgtcgtca ccgtgttgcc gtccgcgtcg cgcgcgtgca
     1861 ccgtagcgag gagtccggtt ccggccgagc ccccaggagg aaaagtcgcg tcgatcgagg
     1921 acggcgcgcc gtgggtcacc gtgatcacgc cggtcgagtc agtgatcgcg ccgttcgcac
     1981 tcaccacgcg tcccgagccg accgtcgtaa gcgtgagaac ggcactcgat gcgggtccgg
     2041 cgggaaccga tccgatacca cccgtcacgc tccacgtccc cgccacgaga ccgagtggat
     2101 gatccgcgac gtcgtatccg cgcaggtaca gagtcgtcgt gtcctggtcc gcgtcgagcg
     2161 caagcgccgc cacttcggct cccgcggcgt cctccacgcg cacgtgatcg agcggtccat
     2221 ccacctcgag cacgggcgac gcgccgatcg gagccgggct cgatcccgcc ggatcgctcg
     2281 agacccagaa caccgccacg tccgggccgg tcggctgcac ctgcgcctgc ggggtgcccg
     2341 ctccatagac cagggtgagc gtgtcccccg tctcgaagcg gctcgtcgag gcgccgccca
     2401 gacggagctc gatcgtgttc ccggcaacca ggatctggtt caccaacgac atgtcggtga
     2461 tctgcacgta gcccggctgc gccggctgtg cgatctgcgg cgcggtccat cccgccggga
     2521 cctgcacgta cagccagcct ccctggggaa acgcgaaatc ttcagcggcg acgtagcgga
     2581 tggtccatgc gccggaagcc cccgcgagaa cgggagcggt gggaacgatc gacgccgtgc
     2641 cgctccccgg tgcggcccac gcgccgaccg gaatgcagca cgatgcgagc aacgtaaagg
     2701 cgagcgtccg cacgacgcgg gcgcgcgcgt tcggcgtagg cagggcagga ctccgttgtt
     2761 tggtgcgggt gtgttgcgtc ccagggactt agcggagggc cggagcgcaa gagacatgcc
     2821 acggggcgga cgcgctcggc gccgcgcgac gagggtgagg tcggcggaag ttcctgtgcc
     2881 gcaaggcttg aatgtggtgg cagttcgaga cgaacgcagg ctcgccgcgg atgccgttgc
     2941 agaatgcacg acgctccggt cgggagcggt cgagatgcgc ggcgcggttg atttcgaacc
     3001 ggcggcccgg attgagccgc ctcggcagcc ctgcctaggc cgtcggcgct accggcgcgg
     3061 ccacctcacg tcgccgacca ccttccccga cccggcgcgc gatccaggtt cgagtccctg
     3121 cggaggcgcc ggcggaagaa tctgctcgag gaggcggtcc tgatccagcg cgcgcttgtt
     3181 cgcgagtccg atgaagagca cctggatgat cgtggcgccc gcgagcacgc cgagtcccac
     3241 gtcgaagacc tctttgagcc gggagcgcat cgcgtcgctc cagtacgcaa gcccgaggag
     3301 aaggagtccc accagcacgc tcgtcacgag tccggtcagc ttgccgaagg ccgtgggctg
     3361 gatcttcacc ggaccgtgga agaggtagag gtagcacgtc ccgaggatca ggatcccgta
     3421 gcgcgcgagc atcagcgccg tgacccacca cggaagcgcg ccgaagaccg tcagcgcgat
     3481 gaagaccgtg gcgttgaaga agacgtcgat cgccgggtcg agcacggcgc cgatgatcga
     3541 ttccatgtgg agcgcgcgcg ccacgactcc gtcgagcacg tcggtgaacg cgctcgccgc
     3601 gaagagccag accacgaccg accagcgccc ctcgcgtgcg aggagcacga tcgcgggcac
     3661 cgccgcgaga cggagcaatg tgagagccac gggcagcggg atgcgcctta gggagcgcac
     3721 ctcgggcgtg cccgcgaggc cgatgtgggc gaggatccag aacgacgcga tgaggagggt
     3781 cacgctcgac gcgatgatgt aggtcactcc cacgcgcctc ccgaacgccc aggagaggag
     3841 gagcgccgcg ccgaactgga tggcgaagag gacgaacgac gtccctgcga cggagcggac
     3901 cagatcggcg cgctccgcga gcgtgccgac gatgcgtgcg aggatgcggc ggaggtaccg
     3961 ggcgaccgag gtgggcgtga aaccctcctt gcggaggtcc tggaggagat cctcggcgaa
     4021 cacggctagg gcacgcggag tgacgcctcg gcgagcaccc gctcggttcc gaacgtggtc
     4081 tcgtcgagcg cgtaggcctc gatcgtgttg gggcgggcgc gtcggaagag aagctgcgct
     4141 tcgatcttgt acggcccctt gtggtcgccg accggcacgc ggtagcgcgc cgtggcgctc
     4201 gcgccggcct tgatgcggcg attctggagc actttcgtgg ccacccaggt ctccacaggc
     4261 caattgccca gctcgtcctg gaggatgacg ccgaacaccg aattggccca ttcggtgaag
     4321 ggatcgttct cccagacgat cgcgccgtcc gcggtgagga cgcgaatccg gacgatcgcc
     4381 atgttctggt cgttgccggt ggggagcgag tggccccagc cggtgttcgt caccaccgcg
     4441 gacagcgaga gcgaatcacc cgacgtggtc ttcgttccgc gcagcacgag cgtcgcggcc
     4501 ggaccgggct gcgccttctc ggcgacctcg gcgctgaagg aatggtcggg caccttcgga
     4561 cgcgacggac cgaggtacga gacacgtccc ggctggccgg gcatgtggca tgtctggcag
     4621 ctcactccct gcttcgcgta cttcgacgcc ttccactccg cgtacgtgtc gctgatcgta
     4681 acgccgttct ggttcttgta cgcgtggcat ccgccgcaga accccgacgt gcgcagatag
     4741 gtggagactt ccgagccgtg cgcgttggtc gggaccggat tccggatgga gccgtagtag
     4801 tggttcagat ccagctcgaa cggcggattg ccggtcgagg ggccgggcgc cgacaccatg
     4861 tggcagaaga cgcaggtcac gccctcgtgc gacacgtcgc gcacgaaatt gaagtcctgg
     4921 ttcacgtagg tggtcgcctc gtggcagggt ccgcagttca ggaacgtgcc gccacgcgtc
     4981 gtcttgagcg actggaaata catccggccg aagagaagat tctgcgaccg ctcggccttc
     5041 gagtgggccg aattctgcca cgattcggtg atcgagagat ggcaggtctt gcaggtctcc
     5101 gccggctgga ggttgacgtc gcgccggaag agcggaggcg gcgtgtagcc gaccgcgccg
     5161 aaggcgagcg cgagcaacag ggcgacgggg aggattcgcg ggttcggggg gctcgttcgc
     5221 aagatggcgg gaatactaca gggtcgaccc cgggggttca aggcgaccgc cctggaatgc
     5281 ttcggcggca atcgtgccca ggatcgcgac ccccgcccgg agcgagcccg acacggtgcc
     5341 cgagatcttc gaccggccga tgcgccggcg gtagcgcacg ggggtttcct cggcccggac
     5401 gccccgccgc gccgccttga cctgcatctc gacggtccac ccgaaggcgc gatcctgcat
     5461 gccgagccgg cgcagggaat cccaccggat cgcccggaac ggcccgagat cggtgtagcg
     5521 cacgccgtag agggaccgaa tgagccgcgt cgcgagccag ttgccgaagc gagcctgcgg
     5581 cgtgagcgct cccggctcgc gcacgccgcg cgtccggctg ccgatgacca gatccgcgcg
     5641 tcccgagcgg atcggctcca cgagcaggtc cgcttcggat ggatcgtcgc tcaggtcccc
     5701 gtccaggaag agaacgatgt cgggcggatc ggacgcgagg gcggcgatgc cgcggaggca
     5761 ggcggcgccg tagccgcgct cgggctccga cacgacgagc gcgccgtgtg cgcgcgccac
     5821 gtccgcagtg ccgtcggagg agccgttgtc ggatacgacg gcgcggcgca cgagcggccg
     5881 cgggagctct tcgagcacgc ggccgatcga cgaggcctcg ttgagcgccg gaatgacgca
     5941 gtccacggtg aaaaccgggg gcgaggggct ctcgacgcgc accacggcgg tcacttccgc
     6001 ttgagcgcgg cgcgtgccgc ggcgagccgg gcgagcggga tgcggaaggg gctgcaggag
     6061 acgtagtcga gccccacgtt ctcgaagaag tcgatcgatg cggcgtcacc gccgtgctcg
     6121 ccgcagatcc cgatcttgag gccgggcttg gcccgccgcc ccgcgaacgc cgcccgctgg
     6181 acgagcgtgc ccactccctt cacgtcgatc gagacgaacg gatcgcccgg gaggattccc
     6241 gagctcgcgt attccggaag gaagcgcccc gaatcgtcgc gcgagtagcc gaaggtcatc
     6301 tgggtgagat cgttcgttcc gaacgagaag aagtcggcgt gctgggcgat ctcgtcggcg
     6361 agcaccgcgg cgcgcggcac ctcgatcatc gtgccgatcg tgtaggggat cgtcaccccc
     6421 gccttcttca tcacctcctc ggcgaccgcc tccacctccg cgcgctgccg acgaagctcc
     6481 tccgggtcgc cgacgagcgg gatcatgatc tctggaatga cgcgcttccc cgccttgagc
     6541 acctggactg cggcctcgaa gaccgcgcgc gcctgcatgc gcgtgatctc cggatggagg
     6601 atgccgagcc tgcatccgcg gaacccgagc atcggattga actcgtggag ctcctcgacg
     6661 cgcgcgaggg tgcgctcgat ctctttccgg cgcgcgggcg tccccttctt ccccatctcg
     6721 cgaagctgcg cttcgagctc ggcgcgcttg ggcaggaact cgtggagcgg cgggtcgagg
     6781 gtgcgaatcg tgaccgggca tccctccatc gcgaggaaga ggcccttgaa gtccgcgcgc
     6841 tggagcggga ggagcttggc gagcgccccc tcgtaggctt ggatcgtggg cttggactcg
     6901 cgccgctcgc ggaggagctc cgcgttcaac cgcgcgcgct cggagcgcgg tgcctccgcg
     6961 agctccgctt cgatccgcgc gatcctcacc ttcgcgtccc gcgctcgctg cgcgttcagg
     7021 atcatcgcga ccacgtgcgg gatccggtcg ggcgcgaaga acatgtgctc cgtgcggcag
     7081 agcccgattc cctcggctcc gaggtggcgc gcgatcttcg cgtcgcgcgg cacgtcggcg
     7141 ttggcccgaa cgcggagccg acgctcctcg tcgcaccagg ccatcagccg ctcgaagcat
     7201 tgatagagcg gcgccttggc ggctggaagc tcccccgtga gcacgcggac gacttcggag
     7261 tcggtcgtgg ggacgtcgcc cacgaggacc tcgccggtcg agccatcgag cgagaggaat
     7321 gcgccctcct tgagcttctt gccgccgatc acgagcacgc gctcctcctc gtggatctgg
     7381 agcgcggagc agccgaccac gcagcacttc cccatcccgc gcgcgaccac ggcggcgtgc
     7441 gaggtcatgc ctccggtcgc ggtgaggatc ccctgcgcgg cgtgcatgcc ggcgatgtcg
     7501 tccggcgagg tctcggcgcg caccaggatg acgcgctccg acgccccgcg gcgcaccgct
     7561 tcgtcggacg tgaagacgat cgcccccgcg gcggcgccgg gcgatgccgg gagtcccgtc
     7621 gcgaccttgt cgtgcttctt ccgtgcggcg gggtcgagca cggggtggag cacctgatcg
     7681 aggtgcgcgg gctcgacgcg ctcgatcccc tcgcgcttcg tgaggagtcc ttcctccacc
     7741 atctcgaccg cgatcttgag cgcggcgatc ccggtgcgcg cggcgcgccg ggtctggagc
     7801 atgaagaggc gcccctcctc gaccgtgaac tcgaagtcct gcacgtcgcg gtagtgccgt
     7861 tcgaggcgcg aggtgatctc gcggagctgg ccgtacgcct cggggagctc gcgctccatc
     7921 tcctcgatgg gccgcggcgt ccggatcccg gccaccacgt cctcgccctg cgcgttgcgg
     7981 aggtattcgc cgtagaagcg ccgctccccc gtgccgggat cccgcgtgaa gccgacgccc
     8041 gtcgcggagc tcaggccgag attgccgaag accatcgcct gcacggtgac cgcggtgccg
     8101 agcccttcgg ggattccgtg gatgcgccga tagtggaccg cgcgctccgt gttccacgag
     8161 cggaagaccg cgtcgcgcgc gccggcgagc tggacgcgcg ggtcttgcgg aaagtcgcgg
     8221 cccgtctcgc gcttcacgat gtcgaggaag cggcgggaca gcgcctcgag gcgctccgcg
     8281 gtgagatcca cgtcgagctc gaccttggcg cgagccttct cgcgcgtgag ctcgcgctcg
     8341 aggagcgcgc gcggcacgcc gagcacgacg tcggagtaca tctggatgaa ccggcggtag
     8401 ctgtcgtggg cgaagcgcgg atgaccgctg cgctgcgcga gccccggcac ggtttgatcg
     8461 ttcaacccga ggttgagcac ggtgtccatc atcccgggca tcgagaccgg agcgccggaa
     8521 cgaaccgaga cgaggagcgg attctccgga tcgccgagcc tccgcccgag gtagcgctcg
     8581 agcttccgga gcgcgtcctt ctcctcctgc acggtggact cgaggagacg ccccccggcc
     8641 tcgaagaact gccggcacgc ggcggtggtg atcgtgtaac cgggagggac cggaatgccg
     8701 gcgttggtca tctcggcgag gccggcgccc ttgcctccga ggaggtcgcg catgcgcgca
     8761 ttcccttccg cgctgccggc gccgaaggaa tagacgtgct tcggttgatc cgccggacgg
     8821 ggcgatgcct tcgcggccgc tttcgtcgcg gtctcctgcg cgctcatggt gcagccctcc
     8881 gcgcgcgccg gtcggtcatc ggagcccgcc tccctcgagt cgcgcgaccc cgcggatcga
     8941 tccgacctcg aggccggccg catttcgaag gggctccccg gcgtgctgcg cgagcgcgcg
     9001 ccgttccgca tcgccgagca cgcccagcgc ctcgagcacg gagaccaccg ccggcccgag
     9061 ggcgcgccgc gatccgtcct ccaccttcac cgcgatcccg acgccgatat ccacgaggcc
     9121 gatcgcgtgc accgcctccg ctcccgcctt cgcgaccgcg gacggagtcg cccgcatgag
     9181 gagcgcgtcg agacgcccgg ggcccgcgac cagctccgga tgttcctgca tcgcatcccg
     9241 gatcgcggcg agcgccggaa gcggagcacc gcccgaatcc tgcccgctcg cgaggagcgc
     9301 gaacgcccga gcgagcgtat cgagcggcat gcggggcgtc ggtgcgctgc acccgtcgat
     9361 cgcgggctcg ggatcggtcc agggctcgcc caagagcgag gcgaagcgat cgaagatgtg
     9421 ctgctgcacc ggatggctcg gatcggcgta ggtctcgacg ggcgcgccca ggtagcgcgc
     9481 gaagagcatc atgcccgcgt gcttgccgga gcagttgtgg cggaccggcg tcggctcctc
     9541 gtgcgcgcgg cggagccgct ccgcggtcgc ttcgtcgtac ggaggatgga cgccgcattg
     9601 gagcatcgat tcgtcgacgc ccgcggctct caggatcgtg cgcgcgagcg cggcgtgccg
     9661 gtcggtgccg ctgtgggagc ctgcgatcaa ggcgagggca tcgctcgcga gcccggactt
     9721 ggcgaacgcg cccgactcga cgagaggaag ggcctggaac ggcttgaacg ccgagcgggg
     9781 aaagacgagc gccttgggct cacccgtgaa ggcgaggaga cgaccccggg cgtcgacgac
     9841 cgcgacgtgg ccgcgatgga tgctctcgac acgctcgtcg cggagcacgt gcacgagcgg
     9901 cgtggcgcgc gcgtccgccg ccgccaggtc cactccgcgt gcctgcctct gcgtcaccgc
     9961 caccgttcga tccccacgaa cgcccgaggg cgagctaact gtcgatctgg gccttctgga
    10021 gcttgccgct gtagtcgatg tagaccgact tccactcggt gaacgcgtcg atcacggtcg
    10081 gcccgccttc acggtggccg ttgccggtct ccttcacgcc gccgaaggga agctgcacct
    10141 cggcgccgat cgtcgggccg ttcacgtacg tgatgcccgc ctcgatgtcg cgaatcgcgc
    10201 gcatggcgcg gttcacgtcg gccgtgtaga tcgaggagct caatccgtag ggcgtgttgt
    10261 tgagcacctg gatcgcctcc tcgaacgagg acacggtgat caccgagagc accggaccga
    10321 agatctcttc gacggcgatg cgcatcgacg gcgtcacctg atcgaagatc gtcggcttgt
    10381 agaagtgccc ctgcgcgagc gctccgtctt cggcgggaga gccaccggcc acgagcttcg
    10441 ccccctcggc ctttccgatc tccacgtagc tgtgcacgcg ttccttctgg ccgcgattca
    10501 cgaccggacc cacgtcggtt ccgggctcga gtccgtctcc gagcttgagc ttctgcgcgc
    10561 gctcgatcag ccgcgcgagc atcgcgtcgt gcacaggctt ctcgacgatg aggcggctcg
    10621 tcgcggtgca gcgctggccg gtcgtgccga acgcgcccca gagcacgccc tcgaccgcga
    10681 ggtcgagatc cgcatctgcg agcacgatct gcgcgttctt cccaccgagc tcgagcgaga
    10741 gccgcttgag cgagctcgcc gtgcgctcgg cgatggtctt gccgacctcg gacgagccgg
    10801 tgaaggaaac catcgcgacg ccgggactct ccgagagcgg gacgccgacc gcggagccgc
    10861 cgccatgcac caggttgagc acgccctcgg gcacgccagc ctccacgagg acttcgacca
    10921 ggtggtgcgc ggacgccggg gtgtcggtgg cgggcttgat gaccaccgtg tttccggtga
    10981 cgagcgccgg gaagatcttc caggacggga tggccatcgg gaaattccac ggcgtgatga
    11041 ggccgcagac ccccatcggc tgtcggatcg acatcgcgaa cttgttcggg agctcggacg
    11101 gcgcggtgac tccgaagagg cgccggccct cggccgcgat gtagtaggcc atgtcgatgg
    11161 cctcctggac gtcgccgcgg gtctcggcca gcaccttccc catctcccgc gtcatggcgc
    11221 gggcgaaggc ttccttcttc tcgcggagga tttcagcggc gcggtagatg atctcggccc
    11281 gcttgggcgc cgggacgagg cgccaggact tgaaggccct ctgcgctgcc tggaccgccg
    11341 cctcgacgtc ttccttcccc gactcgggga aggtcccgat gaggtcggag gggcgggcgg
    11401 ggttccggtt ctcgaaggta cggccggtgc gcgccgggac ccaggcgccg ccgatgaggt
    11461 tgaggtagct ccgggcggcc gggtcggccg tccgggtgcg ctcggccggc tcggccgagg
    11521 gagaggcggt ctgggggttc atgcgaagcc tccgggccgc cttgggttta ccggcgggcg
    11581 gcctacttga tgatgacgaa atgccccttc ttgggcgtac ggccgtcggt ggggtgaacc
    11641 gtatagagat agatccctga gaccaccgtc cggccgtcgt cgttcttgag atcccagggc
    11701 acctcgcccg tgactccgcc cggactcgag ttcgggttct gggtgagcac ccgcaggagc
    11761 tcgccgttgg aggtgtagat ccggatcgtc gcaccggccg ggacgttgat gaagtggatc
    11821 tcccgctggt tcggccccag atcccactcg gccgagccct tgtatggatt cgggacgacc
    11881 gcgatgtgct ccatgttgtc ggcgggcggg gtcgccgggg tcaggtagac gagctgggag
    11941 ctgtcgatgg ggctctcggc gaagtcggcg ttcacgctcg tggtggtgtc aatggatgtt
    12001 accgcgtatt cgtggcggaa ccccgcgagc ggggccagat ccacgaagat ccgggccgtg
    12061 gtgtccacgt ccgccgggtt ccccggcggg tagtcgagaa tgtagcttcc atccggtaac
    12121 ttattgttat aaaatcctct aaagaagatt ccagtgcccg tgaagacgaa attctgaacg
    12181 tcgcaggggg cctgctgggc gaggcagact gggcccacgc ggtccctggc catccattgc
    12241 ccaaccaact ccaagcggct cggggagata ccctcgatcg tccggcggac ccgatagccg
    12301 ttccggaccg gcacgcactg gggggtgcag acggagtcgt actcgacgct cgagctccac
    12361 aggatccaga cgaacgtccc ggtctggttg ttgacctgat tcgccgtatg tgtctggaga
    12421 taatagcctt taacccctgt cgggctggtc agagtctggc cgggggcggg ggctgcgtgg
    12481 gcgagaagga cggtgcaaag agacatgacg aagccgagcc tgaacagcgg gttcctgaac
    12541 atcaacctcc ctcacacgtc ggcggccccg gcggcgcacc ggtccagcaa tagatatccg
    12601 attggggggg tgccgtgcaa ttggggcttg cggaagtagc ccaccggccg gcccgagcga
    12661 aacgggctcg ctggcccgac ccgagcggca gactagggct ccaagtgacg gtgtgtcaag
    12721 gcatttttga acagccgcta ccccgataag ttccaaaaat cccttgacag taggggcgta
    12781 tcgcctgtat actcaatcga gtaaaaaaac ggggcaaccc tttcccattg tgagaaccaa
    12841 catcgtctta gctcgattcg aatcacgatc ccgtaaggag gtgattaggc tcgtagtgtc
    12901 gtctacctta tcctcttgag gtgtcttctc gcttttacct gcgttagaga ggagatgaat
    12961 gggatgaaga aattactagg cgtcatcgga atcctggcgc tggcggccgc gatgcttccg
    13021 gctcaggggc acgcggatct gcgcggttcg ttcgcgccga ttacgatcgc tagcgggttt
    13081 tcccagggat gcgaaaccgc gtctcaccgg cccgacgacg gcaccggcac catgctgttc
    13141 ccggtggacg gcggccgtct cgagcccgag tggatcacgt tcacggcgcg gagcctcgtg
    13201 gtcggccatg agaacgtccg ctcgatttat ctgcacctga atgcccagca gtcggggtac
    13261 ttcgccgtca tcaagacgga cccactggtg ggctcctttg ggcatgacga catctcctat
    13321 ttcgatccgg cctggagcta cgacggcagg ttcctcgcct acatcaagtc tgtgggcaac
    13381 accgccagcg gcctcttcgt gcaggagtat ttcgtttcgg atgacttcga ggccgtgtac
    13441 ggccccggca tgggcgcgga tagcccggtg ggcgcggagt ggctcgtcac gaacgccggc
    13501 gacccccgtc acccggcttg gcacccgtcg tcctacgtgc tcgcgtacga cacttcggtt
    13561 ggcacctcgc gcgacatcta cacgcagacc atcgacgtgg gtcccggaca tatcgacggc
    13621 cctccggttc gcgtgacctt cgacaacgcc aaggccgagc aggatccggc ctggtcgccg
    13681 aatggcaccg acatcgccta caccacgaac aagttcggcc cgctccagat cgagatcgtg
    13741 aacacctcgc tcccgccggc cgatccgaac tacacgcacc tcgccgagcc gaacttcgcg
    13801 ttcgtcacgc acaacaatcc ttcgtacgac ccaacgggcg cccagctcct ctatgacgcc
    13861 ccgggtggcg agaacgtgaa caacgtggtt tcgatctgga ggctcgacct ggcgacgaag
    13921 gccaagtgcg agatgcagct cgacaaccgc gccgatgcgg atccctcgtg gtccccgttc
    13981 gtgcagcaca cgcgggctcc cgagagcctc ccgtactcgt acttcgtcat gacgtcccag
    14041 ggcggcaact tcggcgtgaa catctggcgt gcgaacctga tcaacagctg cctcacgccg
    14101 ctgccgatgg ccgtcacgat caccccgaac gtgatcaact ggaagtcgga gaacaagggc
    14161 aacaacgacg gcgtgccgtt cgtcaccaag atgaagtacc cggctgagac gaaggcgctc
    14221 ggttacgtct gccgctcggc caacacgggc ggcgaaggca ctcgtctccg ttcgaccatc
    14281 ttcaacagcc cgacgctcct caacctctcg tgcccgacca acgcggatct ccacccgcag
    14341 ggtctcacgg gcgtcccgaa caccgagtgc ttcgacacgg cggacacgct cagcgtgagc
    14401 ccgctcacgc tcgagcgcac gatcacctgc tggttcgacc gtcgcacgat cctcgaccag
    14461 atcaccgcgt tgggcctagt cgacaagcag gttccgatgg agatgacggc ctactcgaac
    14521 ctgacgggcc gcaagttcca gggcttcgcc tacatcaagg tcaacaaggt gtccggcgct
    14581 tcttccaacg ttgcgatgat cagcaactcg ccgaacccct tcaacccggt cacgcagatc
    14641 aagtttgcgg tctcgaaggc cggcacgtac tcgctccgca tctacaacgt gcagggcgcg
    14701 ctggtgaagt cgctggccag ccagcggtat gacatcggca cccacgaagc cacgtgggac
    14761 ggccgcacca acaccggtgg caa
//
LOCUS       DASTKC010000248         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1160800, whole genome shotgun sequence.
ACCESSION   DASTKC010000248 DASTKC010000000
VERSION     DASTKC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160800"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR25_10490"
     CDS             <1..258
                     /locus_tag="VFR25_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET9941514.1"
                     /translation="ASGTGVVATTEQVAEAHVWVGEELTRLGRSATPVLYGGSVDPKT
                     ALPLAQLKEVDGFLVGGASLDAPSFLAIAKALAAAVEPARD"
     gene            323..925
                     /gene="secG"
                     /locus_tag="VFR25_10495"
     CDS             323..925
                     /gene="secG"
                     /locus_tag="VFR25_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET9941515.1"
                     /translation="MSGILMGLITTLHILGCVSLMVAILLQSGKGGGLAGTFGAGSSQ
                     TLFGGRGAATFLSRAAGTLAVVFFLTSLTLSLTSTRQSVAGGRSLITEEAKKRAGQGQ
                     RPAESNAPPPLGGATTPGQGLTPPAATPQTTPPATAPAPTATKPSTVPVKRAPAAQPP
                     AASTTQPPAQQAPATNAPSTNTTAPTPPATEGNTTTQGGQ"
     gene            938..1024
                     /locus_tag="VFR25_10500"
     tRNA            938..1024
                     /locus_tag="VFR25_10500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:973..975,aa:Leu,seq:gag)
     gene            complement(1111..1335)
                     /locus_tag="VFR25_10505"
     CDS             complement(1111..1335)
                     /locus_tag="VFR25_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941516.1"
                     /translation="MPEQAELVSNPAVECLRCSKLFGPTQILHLREDPRRSYVRCQHC
                     GGRNEVRAEDRFTIRLVGIIPEPPASRAGA"
     gene            1443..2270
                     /locus_tag="VFR25_10510"
     CDS             1443..2270
                     /locus_tag="VFR25_10510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9941517.1"
                     /translation="MSATAGAYDARRAASYWSGERHHLGDELASVLSLGEPPAVNQAY
                     DAWETGLVLRTLGEGAGRLGLDVGAGIGRVAIRLAPFLGRVACVDLAPGMLERLRANA
                     ASAGVANADPLRSRSDSLPFASASFDAAVCLGLLEHLPRPVQSATIAEIARVLRPGGL
                     FWLVLNNPESTLLTDTADNPYRDGLQRESGYYCSVVSEPALLEEAAAFFRIENLGSNL
                     VYSLQRHAARMLPDASRRDPRLAPFFARAAEWDLALRPVGRMARAAADHHLYGLVRR"
     gene            2267..3241
                     /locus_tag="VFR25_10515"
     CDS             2267..3241
                     /locus_tag="VFR25_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9941518.1"
                     /translation="MSDDKPILVTGGAGYIGSVLVPMLLEKGHRVRVLDRFLHGGHGL
                     ESLNGEGNLEVLIRDLRDPRVHDEAIEGVDTVIHLAAIVGDKACAQNEEVAVQTNWTS
                     TVAFARRAAASGVRRFVFASTCSVYGEGRDEILTEESPVRPLSLYAETRHHAEEGLLE
                     LVGMQGFEPVLLRFGTVYGLSPRMRFDLVVNLLTLHAVKRGKVSIFGGKQWRPFVHVR
                     DIARALEMGMTEAPPPNRPILNVGDNLENYQLLDLREELEARVPGVRIDVVPEASDPR
                     TYRVSFDRIEKLWGFRASRRVGDGIEEIAHALKGGAIPDPESKRYVNA"
     gene            3351..>3890
                     /locus_tag="VFR25_10520"
     CDS             3351..>3890
                     /locus_tag="VFR25_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HET9941519.1"
                     /translation="MDELRERALFVRRDIVTLLARSQSGHSGGPLSTADFGTALFFHE
                     MDVNPEKLDDPSRDLWHFSIGHVTPVIYALMAERGYFPLADLLGFRQFKGHLQGHPSK
                     HDTPGIEVSSGSLGQGLSVCCGMAYGSRFDKNPRRVYCVMGDGEQQEGQIWEASMFAA
                     HYKLDDLCAMIDYNRKQIDG"
BASE COUNT          593 a         1329 c         1343 g          625 t
ORIGIN      
        1 gccagcggca ccggggtcgt cgcgacgacc gagcaggtgg ccgaggcgca cgtctgggtc
       61 ggggaggagc tgacacgcct cggccggagc gcgacgcccg tgctctacgg tggcagcgtg
      121 gatccgaaga cggcgttgcc cctggcccag ctcaaggaag tggacggctt cctggtgggc
      181 ggcgcgtccc tggacgcccc gtccttcctc gcgattgcca aggcgttagc cgcggcggtc
      241 gagcccgccc gcgattgacc cccgccaggg tgcgtggtag tctggtcggc cacacgcgtc
      301 cacgaacatc tcggaggagt tcatgtcggg cattctgatg ggcctcatca cgacgctcca
      361 catcctgggc tgcgtctcct tgatggtggc catcctgctc cagtcgggga agggaggcgg
      421 tctcgccggg acgttcggcg cgggatccag ccagaccctc ttcggcggac gcggggcggc
      481 gaccttcctg agccgcgccg ccgggaccct ggcggtcgtg ttcttcctca cttcgttgac
      541 cctgagcctc acctcgacgc gtcagagcgt cgccggcgga cggagcctca tcaccgagga
      601 ggcgaagaag cgagcgggcc aagggcagcg tcccgcggag tcgaacgcgc cgccgccttt
      661 gggcggagcg accacgccgg gtcagggcct gacgcctccg gctgcgacgc ctcagaccac
      721 gcctccggcg acggctcccg cgcccacggc tacgaagccg tccacggtgc cggtgaagag
      781 ggcgcccgca gcgcagcctc cggccgcgtc gaccacgcag ccgccggcgc agcaagcgcc
      841 cgcaacgaac gcgccgtcga cgaacacgac cgcaccgact ccgcctgcga ccgaagggaa
      901 cacgacgacg cagggcggtc agtaatcgta tgcatcagcc cgggtggtgg aatctggtag
      961 acacgtacgt ttgaggggcg tatgggtaac ccccgtgcga gttcaaatct cgccccgggc
     1021 accacaatgg gtcccctttc cttcgtgaaa ggggacccgt ttttttatgc ggtagagcgg
     1081 ccgccgtgcg gtcgcgaggg tgtggagtgc ttacgcgccg gcgcgcgagg cgggaggctc
     1141 ggggatgatg ccgacgaggc ggatggtgaa gcgatcctcg gcgcggacct cgttcctccc
     1201 gccgcagtgc tggcagcgaa cgtaggagcg gcgaggatct tcgcgcagat gaaggatctg
     1261 ggtcggtcca aagagcttcg agcaccggag gcactcgacg gcggggttgc tgaccagctc
     1321 ggcctgctcg ggcattgcgg ttcctccagg attcggatgg gcgtggcgcg gcaccgcgcg
     1381 tcctcgtacc ggggcattgt aggcgcgccg ggcggcgcgg tccagcccgc gggggtgccc
     1441 gcgtgagcgc cacggccggt gcctacgacg cccgccgcgc cgcctcgtac tggagcggcg
     1501 agcgccacca tctcggggac gaactcgcgt cggtgctgag cctcggcgag ccgcccgccg
     1561 tcaaccaggc ctacgacgcc tgggagaccg gcctcgtgct ccgcacgctc ggcgaaggcg
     1621 ccggacgcct cgggctcgac gtcggagccg ggatcggccg cgtcgcgatc cgcctcgcgc
     1681 cgttcctcgg gcgcgtcgcc tgcgtcgatc tcgccccggg catgctcgag cgtctccgcg
     1741 cgaacgccgc gagcgccggt gtcgcgaacg ccgacccgct ccgctcgcga tcggactcgc
     1801 tcccgttcgc gagcgcgtcc ttcgacgcgg cggtctgcct cggtcttctc gagcatctgc
     1861 cgcgtccggt ccagagcgcg acgatcgccg aaatcgcgcg cgtgctccgt ccgggcggcc
     1921 tcttctggct cgtgctcaat aatccggaga gcacccttct cacggacacc gccgacaatc
     1981 cgtatcgcga cgggctccag cgggagagcg gctactactg ctcggtcgtg tccgagccag
     2041 ccctcctcga ggaagccgcg gcattctttc ggatcgagaa cttgggctcg aacctcgtct
     2101 actcgctcca gcgtcacgcc gcgcgcatgc ttccagacgc ctcacggcgg gatccgcgcc
     2161 tggccccgtt cttcgcacgc gcggccgagt gggacttagc acttcgcccg gtcgggagga
     2221 tggcccgcgc cgccgcggat caccacctct acggactcgt gcgccgatga gcgacgacaa
     2281 gccgatcctc gtcacaggcg gcgccggcta catcggctcg gtgctcgtcc cgatgctcct
     2341 cgagaagggc catcgggtgc gcgtgctcga ccggttcctt cacggcggac acgggctcga
     2401 atccctgaac ggcgagggca atctcgaggt cttgatccgc gacctccgcg atccgcgtgt
     2461 gcacgacgag gcgatcgaag gcgtcgatac ggtgatccac ctcgcggcaa tcgtcggcga
     2521 caaggcgtgc gcccagaacg aggaagtcgc ggtccagacc aactggacct ccacggtcgc
     2581 gttcgcgcgc cgcgccgccg cgagtggcgt ccgccggttc gtgttcgcct ccacgtgcag
     2641 tgtttacgga gagggccggg acgagatcct gaccgaggag tcgccggttc gcccgctgtc
     2701 cctgtacgcc gaaacgcgac atcacgcgga ggaggggctg ctcgagctcg tagggatgca
     2761 agggttcgaa cccgtgctgt tgcgattcgg gaccgtgtac gggctttctc ctcggatgcg
     2821 attcgacctc gtcgtgaacc ttcttacgct ccacgcggtc aagcggggga aggtctcgat
     2881 cttcggcggc aagcaatggc gccccttcgt gcacgtgcgc gacatcgcgc gggcgctcga
     2941 gatgggaatg accgaagcgc cgccgccgaa tcggccgatc ctgaacgtgg gggacaacct
     3001 cgagaactat cagctcctcg atctccggga agagctcgag gcgcgggtcc cgggcgtgcg
     3061 aatcgatgtc gtgcccgagg cgagcgatcc gcgaacctac cgtgtctcct tcgatcggat
     3121 cgagaagctg tggggattcc gcgccagccg gcgcgtcggg gacgggatcg aggagattgc
     3181 ccacgcgctc aagggtggcg cgatcccgga tccggagagc aagcgctacg tgaacgcctg
     3241 agcgttgcgc cggtagggtc ttgcgccctg tgccagcgcg ggttagggtc aatcgatacg
     3301 agcaggtcgg ctccgcggga ggtactcctt tgccacgcct acagttgagt ttggacgaac
     3361 tacgcgagcg cgcgcttttc gtccgacggg acatcgtcac cctcctggcc cgctcgcaga
     3421 gcggccatag cggcggtccc ttgtcgacgg ccgacttcgg gacggcgctc ttcttccacg
     3481 agatggacgt gaaccccgag aagctggacg atccgtcccg cgacctctgg cacttctcga
     3541 tcggccacgt cacaccggtc atctacgcgc tcatggcgga gcgtggctac ttcccgctcg
     3601 cggatctgct cggctttcgc cagttcaagg ggcacctcca ggggcatccg agcaagcacg
     3661 acactccggg aatcgaggtc tcctcgggct cgctggggca ggggctctcg gtctgctgcg
     3721 gcatggcgta cggatcgcgc ttcgacaaaa atccgcgtcg cgtctactgc gtgatggggg
     3781 acggcgagca gcaggaaggg cagatctggg aggcgtccat gttcgccgcc cactacaagc
     3841 tcgacgacct ttgcgccatg atcgactaca accggaagca gatcgacggc
//
LOCUS       DASTKC010000249        12798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1399738, whole genome shotgun sequence.
ACCESSION   DASTKC010000249 DASTKC010000000
VERSION     DASTKC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12798
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399738"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /gene="atpH"
                     /locus_tag="VFR25_10525"
     CDS             <1..385
                     /gene="atpH"
                     /locus_tag="VFR25_10525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET9941520.1"
                     /translation="TETFLEAQRSRLGRPVSGLLHVLLKRRRLDHLVAIAEEFEKLAE
                     KAQGITHATVRTASPITDAQADRLTQVLMRRMGGTIQLTREVDPSLLGGAAVSLDHKV
                     IDGTLASELRRIRRALLQARIPGRG"
     gene            389..1912
                     /gene="atpA"
                     /locus_tag="VFR25_10530"
     CDS             389..1912
                     /gene="atpA"
                     /locus_tag="VFR25_10530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9941521.1"
                     /translation="MAFKPEEVSSVLEQELEKYESRLTQESVGTVLQVGDGIARVWGL
                     DDAMAGELVLFPGDVTGVVLNLEEDNVGCALFGTTQSIKQGDTVRRTSRLASVPVGDA
                     MFGRVINAIGEPIDGKGPIVTTDSRPLEGRAPGVVDRQPVNQPVQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTAVAIDTILNQKGTGIRCIYVAVGQKDFTVAKVVKILQDGGAM
                     EYTTVVAAGASESAPLQYIAPFTGCAIGEDVRDRGGHALVIYDDLSKHAAAYRQLSLL
                     LRRPPGREAFPGDVFYLHSRLLERAAKLNDPLGAGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYSGVRPAINVGISVSRVGGKAQTKAMKKVAGRLRLDLAQYREL
                     AAFAQFGSDLDKTTQAQLTRGERMVELLKQGQYQPLSVERQVAIIYAGTNGFLDDLPV
                     TSVRPFEQGFYEYLDKEYADLMHELRAKYELTDAGEGKLKKAIEAYKAVFVKSPKKA"
     gene            1917..2771
                     /gene="atpG"
                     /locus_tag="VFR25_10535"
     CDS             1917..2771
                     /gene="atpG"
                     /locus_tag="VFR25_10535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET9941522.1"
                     /translation="MATLRDIRRRIRSVRSTAQITKTMEMVSAAKLRRAQTALESARP
                     YDQELKKVLANLASASGDSLTPVFVRREVKRRALIVITSDRGLSGAFNVNLIKAAEGR
                     LREGGAPIALVTLGRKGADYFRRRNAAVLHSNTEIGGVASWKMAEELSRDLTARFLAG
                     EIDEVDLVFTHFKSALFRSIVIEPFLPLGRTETAETVQTLDYIFEPSPEAILEKIVPY
                     YLAMRLYMALAESAASEHGARMVAMGSATKNANEMIQNLTLHMNRTRQATITRELVEI
                     VAGAEALK"
     gene            2782..4224
                     /gene="atpD"
                     /locus_tag="VFR25_10540"
     CDS             2782..4224
                     /gene="atpD"
                     /locus_tag="VFR25_10540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9941523.1"
                     /translation="MATATTNGSGKNVGQIIQVIGPTVDVRFEPKRLPAINSALLIED
                     AEKGIHLTVEVALHIGDEVVRCVAMASTDGLVRGMAAVDTGGPITVPVGRQTLGRVFN
                     LLGDPIDQGAPVQEPGKRLPIHRSPPAFDEQETVARIFETGIKVVDFLAPYAKGGKIG
                     LFGGAGLGKTVILQELIRNIATEHGGFSVFSGVGERTREGNDLWLEMKESGVIDKTVM
                     VFGQMNEPPGARLRVALTGVTMAEYFRDEENQDVLFFVDNIFRFTQAGSEVSALLGRM
                     PSAVGYQPTLATEMGALQERITSTKKGSITSVQAIYVPADDLTDPAPATTFTHLDATT
                     VLSRRIAELGIYPAVDPLDSTSRILDPNIVGEEHYNLARKVQTILQRYKDLQDIIAIL
                     GMDELSEDDKLIVTRARKIQRFLSQPFFVAEPFTGTPGRYVKLEDNIRSFRDIVNGKC
                     DHIPEQAFYMVGTIDEVYQRAEQMGVGAAA"
     gene            4231..4665
                     /gene="atpC"
                     /locus_tag="VFR25_10545"
     CDS             4231..4665
                     /gene="atpC"
                     /locus_tag="VFR25_10545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET9941524.1"
                     /translation="MADTPFTFRLVTPQRLLLEAEVTSLQVPGSEGYLGVLAHHAPLI
                     TALRPGRLDVRDTKGAMQSYAVSGGFLEVSGNRATVLADTAERKGEIDVARAEACLKR
                     AEERLRSESGSGHGGGVATAIDKERAQRALERAKNRLHVARG"
     gene            4674..5690
                     /gene="tsaD"
                     /locus_tag="VFR25_10550"
     CDS             4674..5690
                     /gene="tsaD"
                     /locus_tag="VFR25_10550"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9941525.1"
                     /translation="MRVLGLETSCDDTGAAILDDGRLIAHVVESQDVHVTYGGVVPEY
                     ASRAHITLLPRVLQATLEKAGASLDSIDAVAATVGPGLVGSLLVGLEFAKGLAMARGI
                     PFVGVNHVEAHLVSPGLENELPYPFLGMVASGGHTEIYHVERHGSVRRLGSTRDDAAG
                     EAFDKVGKLLGLPYPGGPHVDRLARLGDASAIEFPVAKLERGTLDVSMSGLKTAVKLF
                     VEREPKPIPDKRVRDIAASAERAIVAALIERLEEALDHYPSRALAISGGCACNKLLRE
                     ESARIAKSRGIPSLVPSPPLCRDNGAMIGYAGWLALRRGEATPLTVNAVPNLDSFPSP
                     TPIP"
     gene            5867..6871
                     /locus_tag="VFR25_10555"
     CDS             5867..6871
                     /locus_tag="VFR25_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9941526.1"
                     /translation="MSQPAVPDSARILIIEDEPRLRTILQMQLETAKDARYEVTTAPD
                     GLQGLEAVKHDPPDLILLDVMMPGMDGYDVCKRIKADPLTSRIPIIFLTAKTTLEDRL
                     QGLENFADDYVTKPWDRGELLFRVRNQLKTRRAQLQSNPLTGLPGNVLIETELNRRIQ
                     GKEKFAFLHLDLDFFKAFNDYYGYARGDVLIRFTASLLHDVIERLGGEGDFVGHIGGD
                     DFVVITVPDRSLSIAEAVRTDFVRRVPLQYDEADRERGSISVHSSRQGGVQSFDIMSI
                     TILIVTNVSRDIQHSAQVSDVAKELKKIGKAERGSSIVPDRRSDGSLVVPVRKEGEEA
                     "
     gene            6868..9273
                     /locus_tag="VFR25_10560"
     CDS             6868..9273
                     /locus_tag="VFR25_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9941527.1"
                     /translation="MSKASGGPGARRPGQGLLSSLGSLAFGLRAAYDSAGLVAAVADH
                     VLDITRADTFALLLLDYETGELEGDRFERGRTGPTGRSRFVATPSGFLGRVLRRETLV
                     IESADAPGAPEIEWSGPRPKTLVGLPVVVGTSLLGVAVLGYRKEVALSDRRRRALLFL
                     SDQIGLAVDRLRTRSELDTTRKQLEESALTLRRVDEAKSDLISIVSHELRTPLTAIKA
                     YTETLIDNVGSPTFTMQEKFLQIVNEECDRLSRIVNDVLDLSRMESGRRRLKADSIDL
                     QRLLGEVEPTVLPELTHKSARIHYDIPADLPRVEADPDLLKQVLVNLIVNAAKFTRPD
                     TDVTVRATRHGERMMIAVEDHGRGIPGDKLGRVFDRFYRIEDEGMERVHGTGLGLAIV
                     KSVVELHGGTVRVESVVGEGSRFLVDLPLRQQRFRQLMRSISPFFERTDLKQLLQGCV
                     AMVAEVMEARIVSFMFFDEDGRELVIRSSHGLDPETVANTRVRAGDSVAGWVAQTSED
                     LCVDDIEADRRFRRMNHPQYETKSLLCVPLRVGGETVGVVNVNNKTAGAPFDTDDLSL
                     LVAITRRVGVVIDRVRTAGPAADVSTVLATLRAIVRARRMSVMPSSRSTFKVAIDLGR
                     RLKLDESELEVLGYVARVHDVGMLALGENLLGNSRRWTDSEYRQVESHPQASVQILKP
                     IEFASKVNQLILSHHEHVDGTGYPRGLRGEEIPLGARILAVLDAYEAMTSGRPYRDPF
                     TPAEALDELRRCAGKQFDPRVVEEFGRLLVESGDAPDAGRSDPSARGTTARDRARDRD
                     AVR"
     gene            9260..11842
                     /locus_tag="VFR25_10565"
     CDS             9260..11842
                     /locus_tag="VFR25_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9941528.1"
                     /translation="MPSAELLALGAITAALVVEEVRLRRIRRRQGEKLQNAYVVVRDR
                     NEQLTQAADEMGRLYRNQLLTSRKQSARLKKVLETATSIGSDLALDKVLHQIVHAVRD
                     AVGFKIVLLRVLDERTELFEARAFAGLDRQAIDKLERRGVPRSEFESWLKDEFRVSRS
                     YFISHKANFWPGPGSEYEGFTPDLGECKEGEWHQDDVLFVPLVHRDGSVLGYLSLDDP
                     ADRRIPSREVIETAEILAAHAVVALQNAALYEQIGESMRQLEEATQRGEELNDLKSSF
                     VQIVSHELLTPLTVIRSHVEALQEHVGGMNPKTQHEFLQAVDENSLKLHRLIQSILEL
                     TQLESGRFRMQREPMNVPELVDETIGLLRDAADEKGIVLGSEPVRRDLIVEADRNLVR
                     RVLLNLGNNAIKFTPKGGRVTFRAQLNERSVKLTVEDNGIGIPAEELTKVFEKFHQVD
                     DSLSRRYPGVGLGLFVAKSIVEWHGGEITAESEPGRGSRFVVALPATPGDASVITHSA
                     APGRSVSEHLTRLTVEMVAEIMNARIASLMLVDEEADELYIKAARGLREEVVCGTRVK
                     MGNSIAGWVAQHGTPLLVTNVEEDARFGTRENSHQYETKSLLSVPVKVDGRVVGVINI
                     NNKISMTPFTEDDKALLSSLSERVGRAWRHASEHDETADRVEQASRALAAIIDNARRS
                     RLKLTSGSMATRATAVARRVGLGEDEIQAIAFVASIHDVGMAHIGPPVLHEPGKLDPE
                     TWAQVAQHPARTVEILRPIEFEERVTECIMAHHERMDGRGYPRGLAGDSIPLGARIIA
                     VVDAYESMTSGRPYRQAMPQAEAIRELKRCSGSQFDPKIVEAFCQVLAAEETTKLVRA
                     PEAA"
     gene            11893..12294
                     /locus_tag="VFR25_10570"
     CDS             11893..12294
                     /locus_tag="VFR25_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9941529.1"
                     /translation="MSKGKILVVDDEIYIVHILDFSLGMEGYEVITALDGEQALERLK
                     ADKPDLVVLDIMMPKLDGYEVCKAIKSNAATKGTPVILLSAKGRNVDQKMGFDVGADD
                     YITKPFSPRKLVERINQLLGQAISERPASSA"
     gene            12399..>12798
                     /locus_tag="VFR25_10575"
     CDS             12399..>12798
                     /locus_tag="VFR25_10575"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941530.1"
                     /translation="MRFIRVAVPVPIDDTFVYEFPENEEPEPGLRVLVPFGRRRLWGT
                     IMSEETERPARAIRPIAGIPEPRLTLTPELIDLCRWVAEYYAAPLGEVLHAAAPSPAA
                     LKRRTVAESPADEPGWHASPPPARSELNEDQ"
BASE COUNT         2162 a         4410 c         4309 g         1917 t
ORIGIN      
        1 gacggagacg ttcctcgagg cgcagcgctc gaggctcggg cgtcccgtgt ccgggctcct
       61 gcacgtgctc ctgaagcggc ggcggctcga ccatctcgtg gcgatcgcgg aggagttcga
      121 gaagctggca gagaaggccc agggcatcac gcacgcgacg gtgcgcacgg cgtctccgat
      181 cacggacgcc caggcggacc ggctcacaca ggtcctcatg cggcgcatgg gaggcacgat
      241 tcagctcacg cgcgaggtgg acccgtccct gctcgggggc gcggccgttt cgctggatca
      301 caaggtcatc gacggcaccc tcgcctcgga gcttcggcgc atccgccggg cgctcttgca
      361 ggccaggatt ccaggtaggg gataacgcat ggcattcaag cccgaggaag tcagttcggt
      421 tctcgagcag gagctcgaga agtacgagtc ccggctcacg caggagagcg tcgggacggt
      481 gctccaggtc ggcgacggga tcgcccgcgt ctggggtctc gacgacgcga tggccgggga
      541 gctcgtgctc ttccccggag acgtgacggg cgtggtcctc aatctcgaag aggacaacgt
      601 agggtgcgcg ctcttcggca cgacccagtc gatcaagcag ggggacacgg tccgccgcac
      661 gagccgcctc gcgtcggtgc ctgttgggga cgcgatgttc gggcgcgtga tcaacgcgat
      721 cggcgagccg atcgacggca agggtccgat cgtcacgacc gactcccgac cgctcgaagg
      781 gcgcgcgccg ggcgtggtgg accgccagcc cgtgaaccag cccgtgcaga cgggcctcaa
      841 ggcaatcgac tccatgatcc cgatcggccg cggacagcgc gagctcatca tcggcgaccg
      901 ccagaccggc aagaccgccg tcgccatcga cacgatcctg aaccagaagg gcaccggcat
      961 ccgctgcatc tacgtggcgg tggggcagaa ggacttcacg gtcgcgaagg tcgtgaagat
     1021 tctccaggac gggggcgcga tggagtacac gaccgtcgtc gcggcaggcg cctccgaatc
     1081 cgcgccgctc cagtacatcg cccccttcac ggggtgcgcc atcggcgagg acgtccgcga
     1141 tcgcggcggc cacgcgctcg tgatctacga cgatctctcg aagcacgcgg cggcgtatcg
     1201 gcagctctcg ctcctcctcc gccggccgcc ggggcgcgag gcgttcccgg gagacgtctt
     1261 ctatctccac tcgcgcctcc tcgagcgcgc cgccaagctg aacgatccgc tgggcgccgg
     1321 ctcgctcaca gcgcttccga tcatcgagac ccaggccggc gacgtctcgg cctacatccc
     1381 gacgaacgtc atctcgatca cggacggaca gatcttcctc gagagcgatc tcttctactc
     1441 gggcgtccgt cccgcgatca acgtcggcat ctcggtgtcg cgcgtcggcg gcaaggcgca
     1501 gaccaaggcc atgaagaaag tggccggccg gctccgtctc gacctcgcac agtaccgcga
     1561 gctcgcggcg ttcgcgcagt tcggctccga cctcgacaag acgacgcagg cgcagctcac
     1621 gcgcggcgag cgcatggtcg agctcctgaa gcagggccag taccagccgc tctcggtgga
     1681 gcgccaggtg gcgatcatct acgcgggcac gaacggcttt ctcgacgacc tgccggtgac
     1741 gtccgtccgt cccttcgagc agggcttcta cgagtacctc gacaaggagt acgccgacct
     1801 catgcacgag ctccgcgcca agtacgagct gacggacgcg ggcgagggca agctcaagaa
     1861 ggcgatcgag gcgtacaagg ccgtgttcgt gaaaagtccg aagaaggcct agtcgattgg
     1921 cgacactccg cgacatccgc cgccgcatcc ggtccgtccg gtcgacggcg cagatcacga
     1981 agaccatgga gatggtctcg gcggcgaagc tacgccgggc gcagaccgcg ctcgagtcgg
     2041 cgcgccccta cgatcaggag ctcaagaagg tgctcgcgaa tctcgcgagc gcgtccgggg
     2101 actcgctcac tccggtgttc gtgcgtcgcg aggtgaagcg ccgtgccttg atcgtcatca
     2161 cctcagaccg cggcctgagc ggcgccttca acgtgaacct gatcaaggcg gccgaaggcc
     2221 ggctccgcga gggcggggcg ccgatcgcgc tcgtcacgct cggacggaag ggggccgact
     2281 atttccgccg ccggaacgcg gccgtgctcc actccaacac ggagatcggc ggagtcgctt
     2341 cgtggaagat ggccgaggag ctgagccgcg acctcacggc ccgcttcctc gcaggcgaga
     2401 tcgacgaggt cgatctcgtg ttcacgcact tcaagagcgc gctcttccgg tccatcgtga
     2461 tcgagccgtt cctcccgctc ggccggaccg agaccgccga aaccgtgcag acgctcgact
     2521 acatcttcga gccgtcgccc gaggcgatcc tcgagaagat cgtgccgtac tacctcgcga
     2581 tgcggctcta catggcgctc gccgagtccg ccgcgtcgga gcatggcgcg cggatggtgg
     2641 cgatgggctc ggccacgaag aacgcgaacg agatgatcca gaatctgacg ctccacatga
     2701 accgcacccg gcaggccacg atcacccgcg agctggtgga gatcgtggcg ggcgccgaag
     2761 cgctcaagta ggaaaggaat tatggcgacc gcgacgacca acggcagcgg caagaacgtc
     2821 ggccagatca tccaggtgat cggcccgacg gtggacgtgc gcttcgagcc gaagcgcctc
     2881 ccggcgatca actcggcgct cctcatcgag gacgcggaga agggcatcca cctcacggtg
     2941 gaggtggcgc tccacatcgg cgacgaggtg gtgcggtgcg tggccatggc ctcgaccgac
     3001 ggcctcgtgc gcggtatggc cgcggtcgac acgggcggac cgatcacggt cccggtcggc
     3061 cgccagacgc tcgggcgcgt gttcaacctg ctcggcgacc cgatcgacca gggcgctccc
     3121 gtccaggagc ccggcaagcg gcttccgatc caccgatcgc cgccggcgtt cgatgagcag
     3181 gagacggtcg cccggatctt cgagaccggc atcaaggtcg tcgacttcct cgccccgtac
     3241 gccaagggag gcaagatcgg cctcttcggc ggcgcgggcc tcggcaagac ggtcattctc
     3301 caagagctga tccggaacat cgccaccgag cacggcggct tctcggtctt ctccggcgtg
     3361 ggtgagcgga cgcgcgaggg gaacgacctc tggctggaga tgaaggagtc gggcgtcatc
     3421 gacaagacgg tgatggtgtt cggccagatg aacgagccgc ccggagcgcg tctccgcgtg
     3481 gcgctcacgg gcgtgacgat ggccgagtac ttccgcgacg aggagaacca ggacgtgctc
     3541 ttcttcgtgg acaacatctt ccggttcacc caggcgggct cggaggtgtc ggcgctcctc
     3601 ggccgcatgc cgagcgcggt gggctaccag ccgacgctgg cgaccgagat gggcgcgctt
     3661 caagaacgca tcacctcgac gaagaagggc tcgatcacgt cggtgcaggc gatctacgtg
     3721 cccgccgacg acctcaccga tccggctccg gccaccacgt tcacgcatct cgacgcgacc
     3781 acggtgctct cgcgccgcat cgccgagctg ggcatctacc cggccgtcga tccgctcgac
     3841 tccacgtcgc gaatcctcga cccgaacatc gtgggcgagg agcactacaa cctggcccgc
     3901 aaggtccaga caatcttgca acgctacaag gatctccagg acatcatcgc gattctcggc
     3961 atggacgagc tctccgagga cgacaagctc atcgtcaccc gcgcgcggaa gatccagcgc
     4021 ttcctctcgc agcccttctt cgtcgcggag ccgttcaccg ggacgccggg acgctacgtg
     4081 aagctcgagg acaacatccg ctcgttccgg gacatcgtga acggcaagtg cgatcacatc
     4141 cccgagcagg cgttctacat ggtgggcacg atcgacgagg tctaccagcg cgccgagcag
     4201 atgggcgtcg gggccgcggc ctagcacgcg atggccgaca ctccgttcac gttccgcctc
     4261 gtcacgccgc agcgcctcct gcttgaggcc gaggtcacgt cgctccaggt ccccggatcc
     4321 gagggatacc tgggcgtgct cgcgcatcac gctccgctca tcacggcgct ccgcccgggg
     4381 cgcctcgacg tgcgggacac gaagggcgcc atgcagagct acgccgtgag cggcggcttc
     4441 ctcgaggtct cgggcaatcg cgcgacggtg ctcgccgaca ccgcggagcg gaagggcgag
     4501 atcgacgtcg cgcgcgccga agcgtgcctg aagcgcgccg aggagcgcct ccggtcggag
     4561 tcgggctcag gccatggcgg cggcgtcgcg accgcgatcg acaaggagcg cgcccagcgc
     4621 gcgctcgagc gcgcgaagaa ccggctccac gtcgcgcgcg ggtagaggac gccgtgcgcg
     4681 tcctcggcct cgagacttcc tgcgacgaca cgggcgccgc gatcctcgat gacgggcggc
     4741 tgatcgcgca cgtcgtcgaa tcgcaggacg tccatgtcac ctacggcggc gtcgttcccg
     4801 agtacgcgtc ccgcgcccac atcacgctcc tcccccgcgt cctccaagcc accctcgaaa
     4861 aggcgggcgc ctcgctcgat tcgatcgacg ccgtggccgc caccgtgggc ccggggctcg
     4921 tcggatccct cctcgtcggg ctcgagttcg cgaagggcct cgccatggcg cgcggcatcc
     4981 cgttcgtcgg cgtgaaccac gtggaggcgc acttggtctc gcccggcctc gagaacgagc
     5041 tcccgtatcc cttcctcgga atggtcgcgt ccggcgggca cacggagatc tatcacgtag
     5101 agcgccacgg ctcggtccgg cgtctcgggt cgacgcggga cgacgccgcc ggcgaggcgt
     5161 tcgacaaagt agggaagctc ctcgggcttc cgtatccggg cggcccgcac gtggaccgcc
     5221 tcgcgcggct cggcgatgcg agcgcgatcg agttcccggt ggcgaagctc gaacggggga
     5281 cgctcgacgt gagcatgagc gggctcaaga ccgcggtgaa gctcttcgtc gaacgcgagc
     5341 ccaagccgat tcctgacaag cgcgtgcggg acatcgccgc gtcggcggag cgcgcgatcg
     5401 tcgcggccct gatcgagcgg ctcgaggagg cactcgacca ctatccctcg cgcgcgctcg
     5461 cgatctcggg cggatgtgcg tgcaacaagc tgcttcgcga ggagtccgct cgcatcgcca
     5521 agtcgcgcgg cattccgtcg ctggtcccgt cgccgccact ctgccgcgac aacggtgcga
     5581 tgatcggcta cgcgggatgg ctcgcgctcc ggcgcggcga ggcgacgccg ctcaccgtga
     5641 acgccgtccc gaatctcgac tccttcccga gcccgacccc gattccgtag ctctacgaag
     5701 agccgtggcc gctaaccgca cctagcggca ccgcttaggc gacccacagg ccgccgaccc
     5761 ccgcctgcac cggtttccgt tcccgcttaa gaatcgatcc cgccgggtcg attaatccca
     5821 agagtcgagc caacttccaa cgagtcaggc catcgggagc ggaaacgtga gccagcctgc
     5881 agtgccggat tctgcgcgaa tcctcatcat cgaggacgag ccgcggcttc gcaccatctt
     5941 gcaaatgcag ctcgagaccg cgaaggacgc gcgctacgag gtgacgacgg cgccggacgg
     6001 ccttcagggg ctggaggcgg tgaagcacga cccgcccgat ctcatcctcc tcgacgtgat
     6061 gatgccggga atggacggct acgacgtgtg caagcggatc aaggccgatc cgctcacgag
     6121 ccggatcccg atcatcttcc tcacagccaa gacgacgctc gaggaccggc tgcagggcct
     6181 cgagaatttc gccgacgact atgtcacgaa gccatgggac cggggcgagc tcctcttccg
     6241 cgtgcggaac cagctcaaga cgcgccgcgc gcagctccag tccaacccgc tcaccgggct
     6301 cccggggaac gtgctcatcg agaccgagct gaaccgccgc atccagggga aggagaagtt
     6361 cgcgttcctg cacctcgacc tcgacttctt caaggccttc aacgactact acggctacgc
     6421 ccgcggcgac gtgctcatcc gcttcacagc gtccttgctt cacgacgtca tcgagcggct
     6481 gggaggcgaa ggagatttcg tgggccatat cggcggggac gacttcgtgg tcatcacggt
     6541 ccccgaccgc tccctgtcga tcgcggaggc cgtgcgcacc gacttcgtga ggcgcgtgcc
     6601 gctccagtac gacgaagcgg accgcgagcg cggctcgatc tccgtccact cgagccgcca
     6661 gggcggcgtg cagagcttcg acatcatgtc gatcaccatc ctcatcgtca cgaatgtctc
     6721 ccgcgacatc cagcacagcg cccaggtgtc cgatgtcgcg aaggagctga agaagatcgg
     6781 gaaggcggag cggggcagct cgatcgttcc cgaccgccgg tcggatggat ccctcgtcgt
     6841 gccggtgcga aaggaaggcg aggaagcatg agcaaggcga gcggcggtcc gggcgcccgg
     6901 cggccggggc aggggcttct gtccagcctg ggcagtctcg cgttcggtct gcgcgccgcg
     6961 tacgactcgg cgggcctcgt ggccgccgtc gccgatcacg tcctcgacat cacccgcgcg
     7021 gacaccttcg cgctcctcct cctcgactac gagacgggcg agctggaagg agatcgcttc
     7081 gagcgcggtc ggacgggccc gaccgggcga tcgcgcttcg tcgccacgcc gtccggattc
     7141 ctgggccgtg tgctccgccg cgagacgctc gtcatcgaga gcgcggacgc gccgggcgct
     7201 ccggaaatcg agtggtcggg gccgcggccc aagacgctcg tggggctccc cgtggtcgtc
     7261 ggcacgtcgc tcctcggcgt ggcggtcctc ggctaccgca aggaggtggc cctctccgac
     7321 cgccgccgcc gcgccctcct cttcctgtcc gaccagatcg gactcgcggt cgaccgcctc
     7381 cgcacccgct cggagctcga cacgacgcgg aagcagctcg aggagtcggc cctcacgctc
     7441 cgccgcgtgg acgaggccaa gtcggatctc atctccatcg tctcgcacga gctccgcacg
     7501 cccctcaccg cgatcaaggc ctacacggag accctcatcg acaacgtcgg gagtccgacg
     7561 ttcacgatgc aggagaagtt cctccagatc gtgaatgagg agtgcgaccg gctctcgcgc
     7621 atcgtgaacg acgtcctcga cctgagccgc atggagtcgg ggcgaaggcg cctcaaggcc
     7681 gactcgatcg atctccagcg cctcctcggg gaagtggagc cgacggtcct gcccgagctc
     7741 acgcacaaga gcgcccgcat ccattacgac attccggcgg atctgccgcg cgtcgaggcc
     7801 gacccggatc tcttgaagca ggtgctcgtc aacttgatcg tgaacgccgc caagttcacg
     7861 cgccccgaca ccgacgtgac cgtccgcgcg acccgccacg gcgagcggat gatgattgcc
     7921 gtggaggacc atgggcgcgg cattcccgga gacaagctgg ggcgcgtgtt cgatcgcttc
     7981 taccggatcg aggacgaggg catggaacgc gtccacggca cgggactcgg actcgcgatc
     8041 gtgaagagcg tcgtcgagct gcacggcgga acggtgcggg tcgagagcgt ggtcggcgag
     8101 gggagccggt tcctggtgga cctgccgctc cgccagcagc gcttccggca gctcatgcgc
     8161 tcgatcagtc ccttcttcga gcgcaccgac ctcaagcagc tcctccaggg ctgcgtggcg
     8221 atggtggccg aggtcatgga ggcccggatc gtctcgttca tgttcttcga cgaggacggc
     8281 cgggagctcg tgattcgctc gtcgcacggg ctcgacccgg agaccgtggc caacacgcgc
     8341 gtccgcgccg gggactcggt cgccggctgg gtggcccaga cgtcggagga tctctgtgtc
     8401 gacgacatcg aggccgaccg gcggttccgg cgcatgaacc atccccagta cgagacgaag
     8461 tcgctgctct gcgtgccgct ccgcgtgggg ggcgagaccg tcggcgtcgt gaacgtgaac
     8521 aacaagacag ccggcgctcc gttcgacacg gacgatctga gccttctcgt ggcgatcacg
     8581 cggcgggtcg gcgtcgtgat cgaccgcgtg cggacggccg gcccggccgc cgacgtctcc
     8641 accgttctcg cgacgctccg agccatcgtg cgggcgcgcc ggatgagcgt catgccgagc
     8701 tcgcgcagca ccttcaaggt cgcgatcgac ctgggtcgcc gcctcaagct ggacgagtcg
     8761 gagctcgagg tgctgggcta cgtggcgcgc gtccacgacg tgggcatgct cgcgctcggc
     8821 gagaacctgc tcgggaattc gcgccggtgg acggactccg agtatcgcca ggtcgagtcc
     8881 catccccaag cgagcgtcca gatcctgaag ccgatcgagt tcgcctccaa ggtgaaccag
     8941 ctgatcctga gccaccatga gcacgtcgac gggaccggct atccgcgcgg cctgcgcggc
     9001 gaggagatcc cgctgggagc gcggatcctc gcggtgctcg atgcctacga ggccatgacc
     9061 tcggggcgcc cttaccggga cccgttcact ccggccgagg cgctggacga gctccgacgc
     9121 tgcgcgggga agcagttcga cccgcgcgtc gtcgaagagt tcggccgtct cctggtcgag
     9181 tccggcgatg ctccggacgc ggggcgctcg gacccctccg cccgcgggac gaccgcgcgc
     9241 gaccgcgcga gggatcgcga tgccgtccgc tgagctcctc gcgctcggcg ccatcaccgc
     9301 ggcgctcgtc gtggaggagg ttcgcctccg ccgcatccgg cggcgccaag gcgaaaagct
     9361 ccagaacgcg tacgtggtgg tgcgcgaccg gaacgagcag ctcacgcagg cggcggacga
     9421 gatggggagg ctctaccgga atcagctcct gacgagccgc aagcagtccg cgcgtctcaa
     9481 gaaggtgctc gagaccgcga cctcgatcgg atccgacctc gcgctcgaca aggtcctcca
     9541 tcagatcgtc cacgcggtgc gggacgcggt cggcttcaag atcgtcctcc tccgcgtgct
     9601 cgacgagcgc accgagctct tcgaggcccg ggcgttcgcc ggcctcgacc gccaggcgat
     9661 cgacaagctg gagcggcgcg gcgtgccccg ctccgagttc gagagctggc tcaaggacga
     9721 gttccgcgtg agccgctcct atttcatcag ccacaaggcg aacttctggc ccgggccggg
     9781 gagcgagtac gaggggttca cgcccgactt gggcgagtgc aaggaagggg agtggcacca
     9841 ggacgacgtc ctcttcgtgc ccctcgttca tcgggacggc tcggtcctcg gctatctctc
     9901 cctcgacgat ccggccgatc gcaggattcc gtcgcgcgag gtgatcgaga cggcggagat
     9961 cctggccgcg cacgccgtcg tggcgctcca gaacgcagcc ctctacgagc agatcggcga
    10021 gagcatgcgg cagctcgagg aagcgacgca gcggggcgag gagctgaacg acctcaagtc
    10081 gtcgttcgtg cagatcgtct cccacgagct cctcacgccg ctcacggtca tccggagcca
    10141 cgtggaagca cttcaagagc atgtgggcgg gatgaacccg aagacgcagc acgagtttct
    10201 ccaggcggtc gacgagaaca gcctcaagct gcaccggctc atccagtcga tcttggagct
    10261 gacccagctc gagtccggcc ggttccgcat gcagcgggag ccgatgaacg ttccggagct
    10321 ggtcgacgag accatcggac tcctgcgcga cgccgcggac gagaagggga tcgtgctcgg
    10381 ctccgagccg gtgcgccgcg atctgatcgt cgaggccgac cggaacctgg tccgccgggt
    10441 gctcctgaat ctgggaaaca acgcgatcaa gttcacgccc aagggcggcc gcgtcacgtt
    10501 ccgcgcgcag ctcaacgagc gctcggtcaa gctcaccgtg gaggacaacg gaatcgggat
    10561 tccggccgag gagctgacca aggtcttcga gaagttccat caggtcgacg attccctctc
    10621 gcgcaggtac ccgggcgtgg ggcttggcct cttcgtcgcg aagagcatcg tcgagtggca
    10681 cggcggggag atcacggccg agagcgagcc gggacgcggg tcccgcttcg tggtcgcgct
    10741 cccggccacg ccgggcgacg cctcggtcat cacgcactcc gccgccccgg gccgctcggt
    10801 gtccgagcac ctgacgaggc tcacggtcga gatggtggcc gagatcatga acgcgcggat
    10861 cgcgtcgctc atgctcgtcg acgaggaggc ggacgagctc tacatcaagg ccgcgcgcgg
    10921 gctccgcgag gaggtcgtct gcgggacgcg ggtcaagatg gggaactcga tcgccggctg
    10981 ggtcgcgcag cacgggacgc cgctcctcgt gacgaacgtc gaagaggatg cgcgcttcgg
    11041 cacgcgcgag aacagccatc agtacgagac gaagtcgctt ctgagcgtgc cggtcaaggt
    11101 ggacgggcgc gtcgtcggcg tcatcaacat caacaacaag atctccatga cgccgttcac
    11161 cgaggacgac aaggcgctcc tgtcctcgct ctccgagcgc gtgggccgcg cctggcgcca
    11221 tgcctcggaa cacgatgaga cggcggaccg cgtggagcag gcgtcgcgcg cgctcgcggc
    11281 catcatcgac aacgcgcggc ggagccggct caagctgacc tcgggctcca tggccacgcg
    11341 cgcgaccgcg gtcgcgcggc gggtcggcct gggggaggac gagatccagg cgatcgcgtt
    11401 cgtggccagc atccacgacg tgggcatggc gcacatcggg cctcccgtgc tccacgagcc
    11461 aggcaagctc gatcccgaga cgtgggcgca ggtggcgcag caccccgcgc gcacggtcga
    11521 aatcctgcga ccgatcgagt tcgaggagcg cgtcaccgaa tgcatcatgg cgcaccacga
    11581 gcggatggac ggccgcgggt atccgcgcgg cctcgcgggg gactcgattc cgctcggcgc
    11641 gcgcatcatc gcggtcgtgg acgcgtacga atcgatgacg tcgggccggc cctaccgcca
    11701 ggccatgcct caggcggagg cgatccgcga gctcaagcgg tgctcgggat cacaattcga
    11761 tcccaagatc gtggaggcct tctgccaagt ccttgcagca gaagaaacta cgaagctcgt
    11821 tcgggctcca gaagcagctt gagcggccga tacaactctt gatggtttga tggtcacaag
    11881 gaggtgagtc ggatgtcgaa gggcaagatc ctagtggtcg atgatgagat ctacatcgtc
    11941 cacatcctgg acttcagtct cgggatggag ggctatgagg tcatcacggc cctcgatggc
    12001 gagcaggcgc tcgagcgtct caaggccgac aagccggatc tcgtcgtcct cgacatcatg
    12061 atgcccaagc tcgacggcta cgaggtctgc aaggcgatca agtcgaacgc cgcgaccaag
    12121 ggcacgcccg tcatccttct ctccgccaag ggccggaacg tcgaccagaa gatgggcttc
    12181 gacgtgggcg ccgacgacta catcacgaag ccgttcagcc cgcggaagct cgtggagcgc
    12241 atcaaccagc tcctcggcca ggccatttcc gagcgccccg cttcctcggc ctagattcgc
    12301 cgtagcgcgc tccgcgcgca tcccgccgct tctgcgcccc acgaagtccg aggcgcgcct
    12361 caccgctcgt tccaccccgg tgctaccatg cgcccgcgat gcgcttcatc cgcgtggccg
    12421 tcccggtccc catcgacgac acgttcgtct atgaattccc cgagaacgag gagcccgagc
    12481 ccgggctgcg cgtgctcgtt cccttcggcc ggcgccggct ctggggcacg atcatgtccg
    12541 aggagacgga gcgccccgcg cgggcgatcc gccccatcgc cggcattccc gagccgaggc
    12601 tcacgctcac gcccgagctg atcgacctct gccgctgggt ggccgagtac tacgccgcgc
    12661 cgctcgggga agtgctccac gccgcggcgc cgtctccggc cgcgctcaag cggcgcaccg
    12721 tggcggagtc gcccgcggac gagcccgggt ggcacgcgtc gccgccgccg gcgaggtcgg
    12781 agctgaatga agaccagg
//
LOCUS       DASTKC010000250         8980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1165834, whole genome shotgun sequence.
ACCESSION   DASTKC010000250 DASTKC010000000
VERSION     DASTKC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165834"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFR25_10580"
     CDS             complement(<1..251)
                     /locus_tag="VFR25_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941531.1"
                     /translation="MSHTSHRMRALSALALVLVLSGCTKGGPKGGFTMPPMPAETSVV
                     SRERVFDRFEAVGSIEAGEAITVVSEIDAAVMSLPFHEG"
     gene            complement(248..1804)
                     /locus_tag="VFR25_10585"
     CDS             complement(248..1804)
                     /locus_tag="VFR25_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9941532.1"
                     /translation="MQTQRAEAQAFGINTANPDSVLSDALDKIPGEPLTLADAVHSAI
                     DGGSTLAQRAAAEVVAARGAHMRESGHFDPELFFDGTTGEVKAVRKSPFETNDVVRAR
                     TTSGTGGIRALLPLGTELEASMDGLRTETNSSFATVNPSYDAIGRLSLRQPLLRGFGP
                     GTSSEATATGREEKAALARYDDVRIEVETTVEQVYWDLHAAERDLAVQQLIRDQAQAL
                     ETQAELRARSGLVGPNDVATARVFLAEQEQSLLDREEDLDLTSDRLASLIGRRPKDLP
                     RFRAVDQPPTSFPIEPEETAVTRALDRNLAIRARQEDLAAAQAREKGAAWNRYPKLDV
                     LGSIGGTGLGGTPTDVSFIPGAKPVPVSGDNFDDAFTDVIHREFPTWSAGVSLSIPIF
                     LREGRGEHERLKGETDQARQDLEDARRLLTDDVRTAHRALVRAARRFEVAGQGVEASR
                     EQVRIGILQYNSGRTTAFELVRLGADLATAQQRYSQALVRTAKAAAALRFLTSGAYPA
                     STSTGGNTRP"
     gene            complement(1958..2968)
                     /locus_tag="VFR25_10590"
     CDS             complement(1958..2968)
                     /locus_tag="VFR25_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9941533.1"
                     /translation="MIPHGTPVPAPEPEPSSDELLDAYSRAVVHAVEAVGASVVSIEV
                     RHPEPDSRRSAIPPQPRGGSGSGFVFTPDGFILTNSHVVHGASSILVRFADGSSYAAD
                     LIGDDPETDLAVVRISAPHLIPAILGDSNAIRVGQLVVAMGNPYGFQATVTSGVVSAL
                     GRSLRSRTGRLIDNVIQTDAALNPGSSGGPLVTTQGEVIGVNSAMIMPAQGICFAIAI
                     NTAKLVAAQLIRQGRVRRSAIGLAGQSVALARKIARFHRLPVASGVRVESVEPESPAE
                     RAGLEPGDVIVAFGDTPVHGIDDLQARLTEREVGVSSPLTVVRGVEVVTLDVVPRESK
                     PS"
     gene            3040..3957
                     /locus_tag="VFR25_10595"
     CDS             3040..3957
                     /locus_tag="VFR25_10595"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9941534.1"
                     /translation="MDRESSSAGLSAYEIPLTGRGAISCWRLGGTGERRGVGILHGLG
                     DHAGRYAELGSALLERGFAVEAIDLPGHGKSYGKRGHVGSWLDYRAAVTAWMDRAHAA
                     APDRTWTLLGQSMGAFIALDWALAHPARVERLVLCAPPFQLGFRPSLLKVKAAQALVR
                     FWPGFSQGNMILPSMLSHDQSIVRAHMEDPLVHYRITARLFLEFQFMRASLMKRVGEL
                     KTPTLVIQGGEDHVADPAGSEKWVKMAPEGLVELRLYPGLYHEVLAEVGRTEIIASLI
                     DWLEKPMASRGGPGAPAVGASTPPRASRS"
     gene            complement(3851..5404)
                     /gene="glpK"
                     /locus_tag="VFR25_10600"
     CDS             complement(3851..5404)
                     /gene="glpK"
                     /locus_tag="VFR25_10600"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET9941535.1"
                     /translation="MSAHDGERVLAIDQGTTGTTVLLLDRTGRVTGRGYAELPQHFPR
                     PGWVEHNGDEIWGTCVRAIAAALESDRAARIAAIGVTNQRETTLVWDRATSKPIAPAI
                     VWQDRRTAERCDALRKAGKERAIARRTGLRLDPYFSGTKLEWILAHVKGARARARRGD
                     LAFGTIDAWVLWKLTGGAVHATDPTNASRTLLYDIHRHRWDDELLALFGVAPALLPDV
                     GPSSSEFGRTRGVPGLPDGVPIAGIAGDQQAALFGQGCVSAGGLKNTDGTGCFLMLHT
                     GSRAAPPKGGLLTTVACGPRGEAAYALEGSVFIAGAAVQWLRDGLGIIATAAETEHLA
                     RSVPDAAGTYLVPAFAGLGAPWWRPDARGIWCGLTRGTTRAHLARAALEAIAFQTCDV
                     VDAMGAVAPSKIGRMRVDGGATANDFLMQLQADLLGVAVERPQVVETTALGAAHLAGI
                     AIGWWDRGGAIGDAREAMTTFRPGLARRERERLRSGWREAVSMLLRPARPARRARPSA
                     SRASRSRKR"
     gene            complement(5401..6870)
                     /locus_tag="VFR25_10605"
     CDS             complement(5401..6870)
                     /locus_tag="VFR25_10605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET9941536.1"
                     /translation="MSREGLAIDDILVIGGGIHGVAVARDATLRGLRVVLVEANDLAS
                     GTSSRTSKLVHGGIRYLETGQFGLVREALRERSILLRTASAFVGPVSFLIPHYKGSGR
                     PRAWVDFGIALYSLLARDAPPELRAHGAASSSEALSLEPGLDPSRLTGASRFADAQME
                     DARLCVALARDAASEGLEVLAHTAVVGLAEDGGGWRALLRRGLDGADREVRARFVVNA
                     AGPWSEDVDRLGGPSGVLVRRTRGTHVVVPAVTHGHALLLTSRRDGRVFFVLPWGQHS
                     LIGTTDVDDDRPPEEVSPPAEDIRYLLEEAARALPGLRGVRPVRAFAGVRSLVRSGAM
                     KPWANAREHRIVQGRARITLIGGKYTTHRSLAERVVDRIVEALGARTRPCTTTTRIVP
                     LEQVAHTHRFILPGGLSIAEPTVKYAVSHSFALRLDDVLLRRTRLWLDGRALRAAIEP
                     TARWMAPLLGWSTERTSDEINRMTAMLDQESNAIEEAMR"
     gene            6971..7747
                     /locus_tag="VFR25_10610"
     CDS             6971..7747
                     /locus_tag="VFR25_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9941537.1"
                     /translation="MSALQGSLSLVTGATSGFGEAIARRLAAEGSSLAITGRRADRLE
                     KVAADIRRDHGVEVRTLAFDVRDRAGTERALGGAGTLLAIADILVNNAGLALALESVQ
                     AGDPDDWDQMIDTNVKGLLYVTRAVLPGMVARRRGHVVNIGSVAGHLVYAGGAVYSAT
                     KFAVRAISEALRYDVLGTGVRVTNVEPGLAETEFSLVRFKGDRERAGAVYTGIEPLTA
                     EDVADAVLWAVTRPPHVNVQSILLMPTDQAGPHALHRRPS"
     gene            7799..8641
                     /locus_tag="VFR25_10615"
     CDS             7799..8641
                     /locus_tag="VFR25_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9941538.1"
                     /translation="MISPRRASMALALILGALLSFAPIASPDPPGLAFSSSGQGEPTL
                     VLIHGAGQDRHMWDRVTPLLETRHRVVRVDLPGHGESPQVSPFSLKEVASALDRTLDR
                     EKVKKAVLVGQSYGAYVALEEAAAHPSRASAIVPIDLPTYILADSERIGNLEMLLRDR
                     YPLFVAGVFQQMTKDSSQIDSVVSKAEAVPRDVMSAYFRETWRMDLRPRVKKLKQPIM
                     VVLTDETWSAAESWSSARKRLGYETAGPAIGKRFVGSGHLVPLDQPDSLAAAILGFTA
                     TLPR"
     gene            complement(8662..>8980)
                     /locus_tag="VFR25_10620"
     CDS             complement(8662..>8980)
                     /locus_tag="VFR25_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941539.1"
                     /translation="LATFGPWGNRRNDLSTTLTWTVVREDVEAKRSHQHARITARRGK
                     EKLTLEEDHVLRLWTQEDVDGLLERSPLRLAAVYHDRFDPFPIDVPRTGEFGNLYHVL
                     AHR"
BASE COUNT         1352 a         3160 c         3071 g         1397 t
ORIGIN      
        1 tgaccctcgt ggaacggaag gctcatcacg gccgcgtcga tctcggagac caccgtgatg
       61 gcctcgcccg cctcgatgga gccgaccgct tcgaagcgat cgaacacgcg ctcgcgcgag
      121 acgacggagg tctccgccgg catgggaggc atggtgaagc cccctttggg gccccccttc
      181 gtgcagcccg agagaaccag gacgagagcg agcgccgaaa gcgcgcgcat ccggtgcgat
      241 gtgtggctca tggccgggtg ttcccccctg tggacgtgga cgcgggatag gcacccgacg
      301 tcaggaatcg aagcgcggcg gcggccttgg ccgtccgcac gagcgcctgc gaatagcgct
      361 gctgagccgt cgcgagatcg gctcccagcc gcaccagctc gaacgccgtg gtgcggccgc
      421 tgttgtactg gagaatcccg atcctcacct gctcgcgcga ggcttcaacg ccctgccccg
      481 cgacctcgaa cctccgcgcc gcgcgcacga gcgcgcgatg cgcggtgcgg acgtcgtcgg
      541 taagcaagcg gcgcgcatcc tcgagatcct gcctggcctg gtcggtctcg cccttcaaac
      601 gctcgtgctc gccgcgtccc tcgcggagga agatggggat cgaaaggctc acgcccgcgc
      661 tccaggtcgg gaactcgcga tggatcacat ccgtgaacgc gtcgtcgaaa ttgtcgccgc
      721 tcaccggtac cggcttcgca cccgggatga aggacacgtc cgtcggcgtg ccgccgagtc
      781 ccgtccctcc gatcgatcca agcacgtcga gcttcggata gcggttccag gccgccccct
      841 tctcgcgcgc ctgcgcggcc gcgagatcct cttgccgagc cctgatcgcg aggtttcggt
      901 cgagcgcgcg cgtgaccgcg gtctcctcgg gctcgatggg gaacgaagtg ggcggctggt
      961 cgacggcgcg gaaccgtggc aggtccttgg gtcgccggcc gatcagcgag gccaggcggt
     1021 ccgaggtcag gtcgagatct tcctcccgat cgagtagcga ctgctcctgc tccgcgagga
     1081 acaccctcgc cgtggcgacg tcgttggggc cgaccaggcc cgaccgggcg cggagctcgg
     1141 cctgggtctc gagcgcctgc gcctgatcgc ggatgagctg ctgcaccgcg aggtctcgct
     1201 ccgcggcgtg gagatcccag tagacctgct cgaccgtggt ctccacctcg atccgcacgt
     1261 cgtcgtaacg cgccagcgcc gccttctctt cgcgccccgt ggccgtcgcc tcgctcgacg
     1321 ttcccgggcc gaatccgcgg aggagcggct gacgcaggga caggcgcccg attgcgtcat
     1381 aggacgggtt cacagtcgcg aaggatgaat tggtttcggt cctgaggccg tccatcgacg
     1441 cctcgagctc ggttcccagc ggaaggagcg cccgaatgcc gcctgtgcct gaggtggtcc
     1501 gggcccggac cacgtcattg gtctcgaaag gagacttgcg cacggctttc acctcgcccg
     1561 tggtcccgtc gaagaagagc tcggggtcga agtggccgct ctcccgcatg tgggctccgc
     1621 gggccgccac gacctcggcc gcggctcgct gggcgagcgt cgagccaccg tcgatcgcgg
     1681 agtgcaccgc gtccgcgagc gtgagcggct cgcccgggat cttgtcgagc gcgtcgctca
     1741 gaacgctgtc ggggttggcg gtgttgattc cgaatgcctg agcctccgcc ctttgggtct
     1801 gcaccccaag catccatccc aggatcaaaa tcgcgagtga gactgcccgc gcgcggacca
     1861 gcgcggacga gctacggccg ggcgacgacg aggttcctgc cacgatcttc cctgcccccc
     1921 atgggcgcat gagcgccgga acggtaacgg tcttggatta gctcggcttg gattcgcgag
     1981 gaaccacgtc cagcgtcacc acctcgacgc cgcggaccac cgtgagcggc gatgagacgc
     2041 ccacctcacg ctcggtcagc ctcgcctgca ggtcgtcgat gccgtgcacc ggcgtgtccc
     2101 cgaacgccac gatcacgtcc ccaggctcca ggcccgcccg ctccgccggg ctctcgggct
     2161 cgacggattc gacgcgcacg ccgctcgcga ctgggagccg atggaaccgg gcgatctttc
     2221 gcgcgagggc gacgctctgc cctgcgagac cgatcgcgct ccggcgcacg cgtccctgcc
     2281 gtatcaactg cgctgccacc agcttggctg tattgattgc gatggcgaag cagatgccct
     2341 gggcgggcat gatcatcgct gagtttaccc cgatcacctc gccctgtgtt gttacaagtg
     2401 gccctcccga gctgccggga ttgagcgccg cgtcggtctg gatcacgttg tcgatgagcc
     2461 gcccggtccg ggaccgaagc gaccgcccga gcgcgctcac gaccccggag gtgacggtgg
     2521 cctggaagcc gtagggattc cccatggcca ccacgagctg tcccacgcga atggcgttgg
     2581 aatcgcccag gatcgcgggg atcaggtgtg gcgcgctgat acggacgacg gcgaggtcgg
     2641 tctcgggatc gtctccgatc agatcggcgg cgtacgaaga cccgtccgcg aaccggacga
     2701 ggatgctgga cgcgccgtgg acgacgtggc tgttggtgag gatgaagccg tcgggagtga
     2761 agacgaagcc cgagccggac ccgccgcgcg gctggggcgg tatggcggat cgacgcgaat
     2821 cgggctccgg gtgacgcact tcgatcgaca ccacggacgc acccaccgct tccacggcgt
     2881 ggacgaccgc cctggaatag gcgtcgagca gctcgtctga ggatggctcc ggctcgggag
     2941 ccggaaccgg cgttccgtgc ggtatcatgc ctgcttcaga tgccgcggcc ccccgtttgg
     3001 ttgcgccccg gccctcgaaa ccattgtaag gagccgaact tggacaggga atcgtcgagc
     3061 gcaggactgt cggcgtacga aattcccctc accggccgcg gcgcgatctc gtgctggcgc
     3121 ctgggcggga ccggggagcg ccgcggggtc ggaatcctcc acggcctggg cgatcatgcc
     3181 ggacgctatg ccgagctggg ctcagcactc ctcgagcgcg gcttcgcggt cgaggcgatc
     3241 gatctcccgg gacatgggaa gtcgtacggc aagagagggc acgtggggtc gtggctcgat
     3301 taccgggccg cggtcacggc ctggatggac cgcgcgcacg cggccgcgcc ggaccggacg
     3361 tggacgcttc tcggccagag catgggcgct ttcatcgcgc tggactgggc cctcgcgcat
     3421 ccggcgcggg tcgagcgcct ggttctctgc gctccgccgt tccagctagg gttccggccg
     3481 tcgctcctca aggtgaaggc ggcgcaggcg ctcgtccgct tctggcccgg cttctcacag
     3541 ggcaacatga tccttccttc catgctctcg cacgaccaga gcatcgtgcg cgcccacatg
     3601 gaagatcccc tcgtccatta ccggattacg gcccggctct tcctcgagtt ccagttcatg
     3661 cgcgcgtcct tgatgaagcg cgtgggcgag ctcaagactc cgacgctcgt cattcaagga
     3721 ggcgaggacc acgtggccga tccggccggg agcgagaagt gggtcaagat ggctccggaa
     3781 ggactcgtcg agctacgcct ctatcccggc ctctaccatg aggtgctcgc cgaggtgggg
     3841 cggacggaga tcatcgcttc cttgatcgac tggctcgaga agccgatggc ctcgcgcggc
     3901 gggccgggcg cgccggccgt aggagcatcg acaccgcctc gcgccagccg ctcctgagcc
     3961 gctcgcgctc gcggcgcgcg aggcccgggc ggaacgtcgt catggcctcg cgcgcgtctc
     4021 cgatcgcgcc gcctcgatcc caccatccga tcgcgattcc cgcgagatgc gcggcgccga
     4081 gcgcggtcgt ctccacgacc tgcggacgct cgaccgcgac gccgagcaga tctgcctgga
     4141 gctgcatgag gaaatcgttc gccgtcgcgc cgccgtccac gcgcatacgc ccgatcttcg
     4201 agggcgcgac cgcgcccatc gcgtccacga cgtcgcatgt ctggaacgcg atcgcttcga
     4261 gcgccgcgcg cgccaggtgc gcgcgcgtcg tcccccgggt caggccacac cagatcccgc
     4321 gcgcgtcggg acgccaccac ggagccccca gtccggcaaa ggcgggcacg aggtaggttc
     4381 cggccgcgtc gggcaccgac cgcgcgagat gctcggtctc cgccgcagtc gcgatgatcc
     4441 caagcccgtc cctgagccac tgcaccgccg cgcccgcgat gaacacgctc ccctcgagcg
     4501 catacgcggc ctcgccgcgc ggaccgcacg cgaccgtcgt gaggagtcct cccttcggag
     4561 gcgcggcgcg gcttccggtg tggagcatga ggaagcatcc ggtgccatcc gtgttcttga
     4621 gtcccccggc cgacacgcag ccctgcccga agagcgcggc ctgctggtct cccgcgatgc
     4681 ccgcgatcgg aactccgtcg ggaaggcccg gcacaccgcg cgtgcgtccg aattccgagg
     4741 aggacggccc cacgtcggga aggagcgcag gcgccacgcc gaagagggcg aggagctcgt
     4801 cgtcccaacg gtggcgatgg atgtcgtaga ggagcgtccg gctcgcgttg gtgggatcgg
     4861 tggcgtggac cgcgcctccg gtcagcttcc agagcaccca cgcgtcgatc gtcccgaacg
     4921 cgagatcgcc tcggcgcgcg cgggcgcggg ctcccttcac gtgcgccagg atccactcga
     4981 gcttggtccc cgagaagtag ggatcgagcc tgagtcccgt gcgtcgcgcg attgcgcgct
     5041 cctttcccgc cttcctgagc gcgtcgcagc gctcggcggt gcggcggtcc tgccagacga
     5101 tggccggcgc gattggcttc gaggtcgcgc ggtcccagac cagcgtggtc tcgcgctgat
     5161 tcgtgactcc gatcgccgcg atgcgcgccg cgcgatccga ttcgagcgcg gcggcgatcg
     5221 cgcgcacgca ggtgccccag atctcgtcgc cgttgtgctc cacccagccg gggcgcggga
     5281 agtgctgcgg cagctccgca tacccccgtc ccgtgacacg gcccgttcga tcgagcagca
     5341 ggaccgtcgt cccggtggtc ccctggtcga tcgcgagcac ccgctcgccg tcgtgcgcgc
     5401 tcatcgcatc gcctcctcga tcgcgttcga ttcctggtcg agcatcgccg tcattcggtt
     5461 gatctcatcg ctcgttcgct ccgtggacca gccgagtagc ggcgccatcc atcgcgccgt
     5521 cggctcgatc gccgcgcgga gcgcgcgccc gtcgagccac agacgggtcc ggcgcaggag
     5581 aacatcgtcc agcctgagcg cgaaggaatg gctcaccgcg tacttcaccg tgggctccgc
     5641 gatcgagagc cctccaggaa ggatgaaacg atgggtatgt gccacctgct ccagcggcac
     5701 gatccgcgtc gtggtcgtgc agggccgcgt ccgtgctccg agcgcctcga cgattcgatc
     5761 caccacgcgc tcggcgaggc tccggtgagt cgtgtactta cctccgatca gcgtgatacg
     5821 cgcgcgaccc tgcacgatgc ggtgctcccg cgcgttggcc catggtttca tggcgccgct
     5881 tcgaaccagc gagcgcacgc cggcgaacgc gcgcacgggc cggacgccac gaagcccggg
     5941 aagcgcgcgg gccgcctcct cgagcaggta gcggatgtcc tccgcgggcg ggctcacttc
     6001 ttcagggggc ctgtcgtcgt cgacatcggt tgtcccgatg agcgagtgct ggccccacgg
     6061 aagcacgaag aagacgcggc cgtcgcgccg tgatgtgagc aggagcgcgt gtccatgggt
     6121 cacggcggga accaccacgt gtgtgccccg ggtgcgcctg acaagcacgc ccgaggggcc
     6181 gcccaggcgg tcgacatcct ccgaccatgg gccggccgcg ttgacgacga aacgcgcgcg
     6241 cacctcccga tccgccccgt cgagtcccct ccgaagcaag gcccgccatc cgccgccgtc
     6301 ttccgcgaga ccgaccaccg cggtgtgcgc gaggacctcg agtccctcgc tcgccgcatc
     6361 cctcgcgagc gcgacgcaga gtctcgcgtc ctccatctgc gcgtcggcga agcgggacgc
     6421 gccggtgagc cgcgacgggt cgagccctgg ctcgagcgag agggcttccg aggaggaagc
     6481 ggcgccgtgt gcacgaagct caggcggtgc atcgcgtgcc aggagcgagt agagagcgat
     6541 cccaaagtca acccaggcgc gcggacggcc ggagcccttg tagtgtggaa tgaggaagga
     6601 gaccggcccg acgaatgcgg acgcggtccg gagcaggatc gatcgctcgc gcagcgcctc
     6661 ccgcacgaga ccgaactggc cggtttcgag atagcggatg cccccgtgca cgagcttcga
     6721 cgtgcggctg ctcgtgccgg aggcgagatc gttggcctcg accaggacga cgcgaagtcc
     6781 gcggagcgtg gcgtcccgcg ccaccgccac gccgtggata ccgcccccga tcacgaggat
     6841 gtcgtcgatg gcgagcccct ccctggacac gaccaaccgc aatcgccgca gtctagggga
     6901 cgatttcgga acccgccacc gcgatcggtg ctatcctcgc ccgctctatg gacctgccgg
     6961 agacgatacg atgagcgcgt tgcagggctc cctgtcgctc gtcacgggcg cgacgagcgg
     7021 atttggagag gccatcgccc gccgcctcgc agccgagggc tcgtcgctcg cgatcacggg
     7081 acgcagggcc gatcggctcg agaaggtcgc ggccgacatc cgccgcgacc acggtgtcga
     7141 ggtgcgcacc ctcgcgttcg acgtccggga tcgcgcgggc acggagcgcg cgctgggcgg
     7201 ggccggaacg ctcctcgcga tcgccgacat cctcgtcaac aacgccgggc tcgcgctcgc
     7261 gctcgagtcc gtgcaagccg gcgatcccga cgactgggat cagatgatcg acacgaacgt
     7321 gaaggggctt ctctacgtga cgcgcgcggt gctcccgggc atggtcgcac ggcggagggg
     7381 acacgtcgtg aacatcgggt cggtcgcggg gcatctcgtg tacgcgggag gcgccgtcta
     7441 ttccgcgacg aagttcgcgg tgcgcgcgat ctcggaagca ctccgctacg acgtgctcgg
     7501 cacaggggtg cgcgtgacga acgtcgagcc ggggctcgcg gagaccgagt tcagcctcgt
     7561 ccgtttcaag ggggacaggg agcgggccgg ggcggtctac acaggcatcg agccgctcac
     7621 cgccgaagac gtcgcggacg ccgtgctctg ggccgtgacg cggccgccgc acgtgaacgt
     7681 gcagtcgatc ctcctcatgc cgaccgacca ggccggcccc cacgcgctcc atcgacgccc
     7741 ctcgtagcga cgcaccgcgt tcacgtcggg cccgcactct ggtaaaatag aggcctcgat
     7801 gatctcccct cgccgcgctt cgatggctct cgccctgatc ctgggcgccc ttctgtcgtt
     7861 cgctcccatc gcctcgcccg atccccccgg cctcgcgttc tcttcgagcg gccagggaga
     7921 gccgacgctc gtcctgatcc acggcgcggg ccaggaccgc cacatgtggg atcgcgtgac
     7981 gccgctcctc gagacgcggc accgcgtcgt ccgggtggac ctgccggggc acggcgaatc
     8041 cccgcaggtg agcccgttct ccctcaaaga ggtggcgtcc gcgctggatc ggacgctcga
     8101 tcgagagaag gtcaagaagg cggtgctcgt cggccagtcc tatggcgcct atgtggcgtt
     8161 ggaggaggcg gcggcccacc cgtcgcgcgc gtcggccatc gtgccgatcg acctacccac
     8221 gtacatcctg gccgactccg agcggatcgg aaatctcgag atgctccttc gcgaccgcta
     8281 tccgctgttc gtggccggcg tatttcagca gatgacgaag gactcgagcc agatcgactc
     8341 ggtcgtctcg aaagcggagg cggttccgcg cgacgtgatg agcgcgtact tccgcgagac
     8401 gtggaggatg gatctgcggc cgcgggtcaa gaagctgaag cagccgatca tggtcgtcct
     8461 gaccgacgag acctggtccg ccgccgagtc ctggtcgagc gcgcgaaagc gactgggcta
     8521 cgagacggcg gggcccgcga tcggcaaacg cttcgtcgga tccgggcacc tcgtgcccct
     8581 ggatcagccc gattccctcg ccgccgccat cctcggcttc accgcgacac ttccccgcta
     8641 gaacgacgct cgcgctccgc gctaccgatg cgcgagcacg tgatagaggt tcccaaactc
     8701 ccccgttctc ggaacgtcga tcggaaacgg atcgaagcgg tcgtggtaca ccgccgcgag
     8761 tctgagcggg cttcgctcga ggagaccgtc cacgtcttcc tgcgtccaga ggcgaagcac
     8821 gtggtcttcc tccagggtga gcttctcctt gccgcggcgc gcggtgatcc gggcgtgctg
     8881 gtgcgagcgc ttcgcctcca cgtcctcgcg caccaccgtc caggtgagcg tcgtcgagag
     8941 gtcgtttcgg cgattgcccc agggaccgaa ggtggcgagc
//
LOCUS       DASTKC010000251         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1402840, whole genome shotgun sequence.
ACCESSION   DASTKC010000251 DASTKC010000000
VERSION     DASTKC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402840"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFR25_10625"
     CDS             <1..412
                     /locus_tag="VFR25_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9941540.1"
                     /translation="AAVTRLTALLARQFGSHMAAEDEVLYPALMNALPETRSSIEPLS
                     ADHGALRMMLADLEETLGLPGEARDEQMTVQIRDLVDLLRIHIRKEEALVIGVAERVL
                     QPAEVEALSVRMNRGTAASCELGLRPGPSKGVTS"
     gene            409..885
                     /locus_tag="VFR25_10630"
     CDS             409..885
                     /locus_tag="VFR25_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9941541.1"
                     /translation="MKGWVMWTIGMAAALSLAGCGGAKDGEGPAASATSPHDTGPRAA
                     EAPVDDELAEKGEALFKSKTCSTCHAFGARITGPDLAGVTHRRSAEWMENQMLHPDVM
                     TKEDPIARELLGKYAVQMPNLMLKPEEVKALIEYAKKKDKELEAAGKLTAANTQGR"
     gene            890..2779
                     /gene="nosZ"
                     /locus_tag="VFR25_10635"
     CDS             890..2779
                     /gene="nosZ"
                     /locus_tag="VFR25_10635"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HET9941542.1"
                     /translation="MKRSLGSLVLGLALIAAVLATGCAGRGGGTAAQKVYVKPGEYDT
                     HYAFLSGGHSGQVFVYGLPSCRHITTIPVFTPEPARGYGFDEESKAMLGGFTWGDAHH
                     PGLSETNGDFDGRWLFINDMPHARIARIDLKDFTTHEIYGPIPNISAAHACPYPTPNT
                     EYVFAASRFSAPVPTSKVAKLADYKKDFNGVIAGIKVDPNGHMSMGFEILMPPFDWDL
                     SDAGKGPSMGWQFFTCYNSEEAYDSLEVKASQNEMDYVAAVNWKEAQKAVDAGQGHMI
                     AGAPVLDPKEVPGIVYLIPTPKSPHGCDVNPTGEYICASGKLQAEVSVFSFKKLQASI
                     DAKKFDGEAGGIPILRYEDVLEAKVPVGLGPLHTEFDGKGNAYTSLFLDSQVAKWKVG
                     TWKVTDKIDVYYSIGHLVAAEGCTMHPTGEYLLALDKLSKDRYMSVGPSHPEGAQLID
                     LRGPKMELLYDFPTYPEPHYGQIIAASKVKTEKIYPLAENADPAAVKSAEQARVERKG
                     KRVDAYMLAIRTHFTPDVLHVAQGDTIYFHLTNVEQDDDITHGFGILNSNVDMQVEPG
                     ETKTARWVATKAGITPFYCSNFCSALHQEMQGYIEVAPAGTSIAAVQRPSRKQVEEVA
                     AAIGR"
     gene            2835..>4525
                     /gene="nosD"
                     /locus_tag="VFR25_10640"
     CDS             2835..>4525
                     /gene="nosD"
                     /locus_tag="VFR25_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HET9941543.1"
                     /translation="MAAYWWGSFRSVERRLIVIAALVLLPVFFLPVLPIWTMKLWAPQ
                     YPEGLTLTIYTNAIRGDLQKINTLNHYVGMHPITASDFREFTYLPQLLTLFGFMALVA
                     ALVNRRVIALLGWLAFTGFAVYMFRDYAHWLWHYGHDLDPRAAIKLDAFTPPLIGYSK
                     MANFKVWSLPGPGTWLLGLAWLLGPIAIGLEWRERKVAAAAVATALLALSVVAWPGRS
                     SASEITVAAHPGALADAIARAHEGDTVTLLPGVHAGPVRVDRALVVRGWPGAILEGSG
                     TGSVLEITAPGTVVEDLTVRRSGSRVITADAGVRILQSADVTLRRLTLDDVLYGVYAE
                     RADRLRVEGCRLEGRVRPMDESGSGNGLHLWYVHDAALAQNTVEHFADAIYLSFADRT
                     DVAGNRLQWNGRYGLHTMYCQGTRLTGNLFTRNVAGCAIMFSNNLEVVGNDLISNRGP
                     RTYGLLLRDCSGGTFRKNRLVDNTIAMFLDNSNRNRLLDNLFQDNGWGMLLYSSCAGN
                     VTAGNTFLNNDYPVALDMRHSDNRFDDGRSGNYWGENEAYDLNGDGLSDVPYSPV"
BASE COUNT          818 a         1512 c         1491 g          704 t
ORIGIN      
        1 cgccgccgtg acgcgcctga cggcgctcct cgcgcggcaa ttcgggagcc acatggcggc
       61 cgaggacgaa gtgctctatc cggcgctcat gaacgcgctg cccgagacgc gatcgagcat
      121 cgagcccttg agcgccgatc atggcgcgct ccggatgatg ctcgcggatc tcgaggaaac
      181 gctcggcctg ccgggcgagg cgcgcgacga gcagatgacg gtccagattc gggacctggt
      241 ggacctcctc cggatccaca tccggaaaga ggaagccttg gtgatcggcg tggcggagcg
      301 cgtgctccag ccggccgagg tggaagccct atcggtccgg atgaatcgcg gcacggctgc
      361 ctcctgcgag ctgggcctgc gcccaggacc ttcgaaagga gtgacgtcat gaagggatgg
      421 gtgatgtgga cgatcggcat ggcggccgcg ctctcgctcg caggatgcgg cggtgcgaag
      481 gacggggagg gaccggcagc gagcgcgacc tcgcctcacg acacggggcc gcgcgcagcg
      541 gaggcgccgg tggacgatga gctcgccgag aaaggggaag cgctgttcaa gtccaagacc
      601 tgctcgacgt gccacgcgtt cggggcgcgc atcacaggac cggacctggc cggcgtgacg
      661 catcgccgct cggccgaatg gatggagaac cagatgctgc accccgacgt gatgacgaaa
      721 gaggatccca tcgcgcgcga gctgctcggc aagtatgcgg tgcagatgcc caacctgatg
      781 ctgaagccgg aagaggtcaa ggctttgatc gagtacgcga agaagaagga caaggagctc
      841 gaagcggccg gcaagctgac ggcggccaac acgcaaggga ggtagtgcca tgaaacgctc
      901 gctcggttct ctggttctag gcctcgctct gatcgcggcg gtccttgcga ccggctgcgc
      961 ggggagagga ggcggtacgg cggcgcagaa ggtgtacgtc aagccgggcg agtacgacac
     1021 gcactacgcg ttcctctccg gcggccactc cggccaggtc ttcgtctacg gtcttccgtc
     1081 gtgccgccac atcacgacga ttccggtctt cacgcccgag cccgcgcgcg gctacggatt
     1141 cgacgaggag tcgaaggcca tgctgggcgg cttcacctgg ggcgacgcgc accatcccgg
     1201 cctctccgag acgaacggcg actttgacgg ccggtggctc ttcatcaacg acatgccgca
     1261 cgcgaggatc gcgcggatcg acttgaaaga cttcacaacg cacgagatct acggtccgat
     1321 cccgaacatc tcggccgcgc acgcgtgccc gtatcccacg ccgaacaccg agtacgtctt
     1381 cgcggcgtcc cgcttctcgg cgcccgtgcc caccagcaag gtggcgaagc tcgccgacta
     1441 caagaaagac ttcaacggcg tcatcgcggg catcaaggtg gacccgaatg ggcacatgtc
     1501 gatgggcttc gagatcctca tgcctccctt cgactgggat ctctccgacg ccggcaaggg
     1561 cccgagcatg gggtggcagt tcttcacctg ctacaacagc gaggaggcgt acgactccct
     1621 cgaggtgaag gcgtcgcaga acgagatgga ctacgtcgcc gcggtgaact ggaaggaagc
     1681 gcagaaggcc gtcgacgcgg gccaagggca catgatcgcc ggcgcccccg tgctcgatcc
     1741 caaggaagta cccgggatcg tctacctgat cccgacgccg aagagcccgc atggctgcga
     1801 cgtgaatccg accggcgagt acatctgcgc gtcgggcaag ctccaggccg aggtctcggt
     1861 cttcagcttc aagaagctgc aggcgtcgat cgacgcgaag aagttcgacg gcgaggcagg
     1921 cggaatcccg atcctccgct acgaggacgt gctcgaggcg aaggtcccgg tggggctcgg
     1981 gccgctccac accgagttcg acggcaaggg gaacgcgtac acctcgctct tcctcgattc
     2041 ccaggtcgcc aagtggaagg tcggcacctg gaaggtgacg gacaagatcg acgtctacta
     2101 ctcgatcggc cacctggtcg ccgccgaggg atgcacgatg catccgaccg gcgagtacct
     2161 gctggcgctc gacaagctct ccaaggatcg ctacatgtcg gtcggcccgt cgcatccgga
     2221 gggagcgcag ctgatcgatc ttcgcggacc gaagatggag ctcctgtacg acttcccgac
     2281 ctatcccgag ccgcactacg gccagatcat cgcggcgtcc aaggtgaaga ccgagaagat
     2341 ctacccgctc gccgagaacg ccgatcctgc ggcggtgaaa tcggcggagc aggcgcgcgt
     2401 ggagcggaag gggaagcggg tcgacgcgta catgctcgcg atccggaccc acttcacgcc
     2461 cgacgtgctc cacgtggccc aaggagacac catctacttc cacctgacga acgtggagca
     2521 ggacgacgac atcacgcacg ggttcggaat cctgaactcg aacgtcgaca tgcaggtcga
     2581 gcccggcgag acgaagaccg cgcgctgggt cgcgacgaag gcgggaatca cccccttcta
     2641 ctgctccaac ttctgcagcg cgctccatca ggagatgcag ggctacatcg aggtcgctcc
     2701 ggcgggcacg tccatcgccg ccgtccagcg tcccagccgg aagcaggtcg aagaggtcgc
     2761 ggcggccatc ggccgctgaa tcacgggaga cgcggggccg cacgcgcggc cccgcctccg
     2821 ccgcggagga agccatggct gcgtactggt ggggatcgtt tcggagcgtg gagcgccggc
     2881 tgatcgtgat cgccgctctg gtcctcctgc cggtcttctt cctgccggtg cttccgatct
     2941 ggaccatgaa gctctgggcg ccgcagtacc cggagggtct gaccctgacc atttacacca
     3001 acgccatccg cggcgatctc cagaagatca acacgctgaa ccactacgtg gggatgcacc
     3061 cgatcaccgc atccgatttc cgcgagttca cgtatctgcc ccagctcctc acgctcttcg
     3121 gcttcatggc cctggtcgcg gcgctcgtga accggcgcgt catcgcgctc ttgggctggc
     3181 tcgcgttcac gggattcgcg gtctacatgt tccgggacta cgcgcactgg ctctggcact
     3241 acggccacga cctggatccg cgcgccgcca tcaagctcga cgcgttcacg ccgcccctga
     3301 tcggctactc gaagatggcc aacttcaagg tctggagcct gcctggcccg ggcacgtggc
     3361 ttctcgggct cgcctggctg ctcggaccca tcgcgatcgg gctcgagtgg cgggagcgca
     3421 aggtcgccgc ggccgcggtc gcgaccgcgc tcctcgcgct gagcgtcgtt gcctggccgg
     3481 gacgctcgtc cgcctctgag atcaccgtcg cggcccaccc tggcgcgctc gccgacgcga
     3541 tcgctcgcgc ccatgaagga gacaccgtga ctctccttcc cggggtccac gcggggcccg
     3601 tgcgtgtcga ccgcgcgctg gtcgttcgag gatggcctgg cgccatcctc gaagggtcgg
     3661 gcacgggaag cgtgctcgaa atcaccgccc cgggcaccgt ggtcgaagac ctcaccgtgc
     3721 gaaggagcgg gagccgcgtc atcacggccg acgccggcgt ccggatcttg caatccgccg
     3781 acgtcacgct gcggcgcctc acgctcgacg acgtgctcta cggcgtgtac gccgagcgcg
     3841 cggaccgcct gcgcgtcgag ggatgccgcc tcgagggccg cgttcgcccc atggacgaga
     3901 gcggctccgg caacggtctc catctgtggt atgtccacga cgcggcgctc gcgcagaaca
     3961 cggtggaaca cttcgccgac gcgatctacc tctcgttcgc ggatcggacc gatgtcgccg
     4021 ggaatcgcct ccagtggaac gggcgctacg gcctccacac catgtactgc cagggaaccc
     4081 ggctcacggg aaacctcttc acgcgcaacg tggccggctg cgcgatcatg ttctcgaaca
     4141 atctcgaggt cgtggggaac gacctgatat cgaaccgagg tccgcgcacg tacggcctcc
     4201 tgctccgcga ttgctcgggg ggcacgttcc ggaagaaccg gctcgtcgac aacacgatcg
     4261 ccatgttcct cgacaactcg aaccggaacc ggctcctcga caatctcttc caggacaacg
     4321 gatgggggat gctcctctac tcgtcgtgcg ccgggaacgt caccgcagga aacacgttcc
     4381 tgaacaacga ctatccggtc gcgctcgaca tgcggcactc ggacaaccgc ttcgacgacg
     4441 gccgttccgg gaactactgg ggcgagaacg aggcgtacga cctgaacggc gacggcctga
     4501 gcgacgtgcc gtactctccg gtgag
//
LOCUS       DASTKC010000252         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1167767, whole genome shotgun sequence.
ACCESSION   DASTKC010000252 DASTKC010000000
VERSION     DASTKC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167767"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VFR25_10645"
     CDS             <1..1455
                     /locus_tag="VFR25_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HET9941544.1"
                     /translation="GLVLKEYLLRGLVQRALILVPASLVEQWDQELRRKFDLEFAILK
                     HGTAWWEEPLLLASMDTAKLLRHRDKVASAAFDLVVVDEAHRLKNQKTLAWKFLDRLA
                     PRYLLLLTATPVQNDLHELYNLVQLLRPGLLGTYRTFGREFMRRGDRRMPQNTNRLAT
                     LLGETMIRTTRASTSIRFPKREVTNVHFDLSLPEREIYDDVTRLVRRLIQGQKETKRG
                     AIKLTLLTLQKEIGSSSFAAVPTLGKLLARESPGPARAELEDLLVRARAVDANAKFEG
                     VTRLLGASREKVVIFTQFRATLEFLARSIRALGIRTVVFHGALTLREKEAAIAQFRDR
                     ARVLVSTEAGGEGRNLQFCRLLINYDLPWNPMRIEQRIGRLHRLGQQHDVHVYNFTAR
                     QTVESYVLEIVHEKINMFRSVIGDLDMVLGPYAEAGSFEDAVFSIWAGARGDRDLDRE
                     FSKLGTALLVGRKRYDAVEELDRRLLDGVDHDPS"
     gene            1442..2287
                     /locus_tag="VFR25_10650"
     CDS             1442..2287
                     /locus_tag="VFR25_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941545.1"
                     /translation="MTRRKRGEGRSELPEILAAILEARGATIAKRGTVWEASLPSELA
                     GVLGTESVRLVAAPAGRAARGAEMDAGLTERILLLGRSRGEVVRLVAHTPRPARAVAG
                     VRTWVRLHWRIRYGTDELPEELLVQRLPVGPSGGVLAPPDTAFATPSDEDLPSPDPEL
                     LAGAWMRGLRLLESRIRRKLQPHEDRMRRELNREMRTLSIHYRSLIAEERTGRRKRGE
                     GREAGRILQLKEDWERKLSAAVRQRVLDTEARLVAVALLSVVSDGKAPRAEPSARPAR
                     ARRRV"
     gene            2343..3038
                     /locus_tag="VFR25_10655"
     CDS             2343..3038
                     /locus_tag="VFR25_10655"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941546.1"
                     /translation="MSTHLVKHQKITKRQIKEDPLVTAAFKATAHWERHGSRILVGLG
                     VVALLALLGFFMMQARSKAEEKASGDLFRANLAAMQGDYVSAAPILKEIVDNEPGTAA
                     ARDAMLLLGDSFASQGKPGDAATWYQKYIDKAGSKDRQRIGYLALASALEDAGQFGPA
                     ADAFASSAERSDTDNLRGRAMLGQARCLARAGQTPKAIEIYKKVSLLPQAEVTITNAA
                     DVRLGELAAPTAP"
     gene            3051..>3479
                     /locus_tag="VFR25_10660"
     CDS             3051..>3479
                     /locus_tag="VFR25_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9941547.1"
                     /translation="MPRPILLALVWHLHQPSYHDAETGADLLPWTRLHATKDYRDMVR
                     ALARTPRVRVTFNLTPVLLDQLDAIGAGRSDPYLDVARASAASLTAAQASFLLARFFD
                     VNHERMLNPFPRYRELFERKEAGGAFTADDLRDLQTWFHLA"
BASE COUNT          546 a         1233 c         1178 g          522 t
ORIGIN      
        1 gggctcgtgc tcaaggagta cctcctccgc gggctcgtcc agcgcgcgct catcctcgtg
       61 cccgcgtcgc tcgtggagca gtgggaccag gagctccgcc gcaagttcga tctcgagttc
      121 gcaatcttga aacatggcac cgcgtggtgg gaggagccgc tcctcctcgc gtcgatggac
      181 accgcgaagc tcctccgaca ccgggacaag gtggcgtccg ccgcgttcga tctcgtcgtc
      241 gtcgacgagg cgcaccggtt gaagaaccag aagaccctcg cctggaaatt cctcgacagg
      301 ctcgcgccgc gctatctcct gctcctcacc gcgacgcccg tacagaacga cctccacgag
      361 ctctacaacc tggtccagct cttgaggccc ggtctcctcg gcacctaccg cacgttcggg
      421 cgcgagttca tgcgccgggg cgaccggcgg atgccgcaga acacgaaccg gctcgccacg
      481 ctgctcggcg agacgatgat ccggaccacg cgcgcgagca cctcgatccg gttcccgaag
      541 cgcgaggtga cgaacgtcca cttcgatctg agtcttccgg agcgcgagat ctacgacgac
      601 gtgacgcggc tcgtccgacg gctcatccag gggcagaagg aaaccaagcg gggcgccatc
      661 aagctgacgc ttctcaccct gcagaaggag atcgggtcga gctcctttgc ggccgtcccg
      721 acgctgggga agctcctcgc gcgcgagtcg ccggggccgg cgcgggccga gctcgaggac
      781 ctcctcgtcc gcgcgcgcgc cgtggacgcc aacgccaagt tcgaaggcgt cacgcgcctc
      841 ctcggcgcct cgcgggagaa agtcgtcatc ttcacccagt tccgcgccac gctcgagttc
      901 ctggcgcgct cgatccgcgc gctcggcatc cggaccgtgg tcttccacgg cgcgctcacg
      961 ctgcgcgaga aggaggcggc gatcgcgcag ttccgggatc gcgcgcgcgt cctggtgagc
     1021 acggaggcgg ggggcgaggg acggaacctc cagttctgcc gtctcctcat caactacgac
     1081 ctgccctgga atccgatgcg gatcgagcag cgaatcgggc gactccaccg gctggggcag
     1141 cagcacgacg tgcacgtcta caacttcacg gcgcgtcaga cggtcgagtc ctacgtgctc
     1201 gagatcgtgc acgagaagat caacatgttc cggtcggtga tcggggatct cgacatggtg
     1261 ctcggtccgt acgcggaggc cggctccttc gaggacgccg tcttctccat ctgggccggc
     1321 gcgcgcggcg atcgcgacct ggatcgcgag ttcagcaagc tcggcaccgc gctcctggtc
     1381 ggccgcaagc gctacgacgc ggtcgaggag ctggaccgcc gcctcctcga cggcgtggat
     1441 catgacccgt cgtaaacgcg gcgaaggaag gagcgagctc ccggagatcc tcgccgcgat
     1501 cctcgaggcg cgcggcgcca cgatcgcgaa gcggggcacg gtctgggaag cgtcgctgcc
     1561 ctcggagctc gcgggcgtgc tcgggaccga gagcgtgcgc ctcgtcgccg ctcccgccgg
     1621 ccgcgccgcc cgcggcgcgg agatggatgc ggggctcacg gagcgcatcc tcctcctcgg
     1681 ccgctcgcgc ggcgaggtgg tccggctcgt cgcccacacg ccccgtcccg ctcgagcggt
     1741 cgcgggtgtg aggacgtggg tgcggctcca ctggaggatc cggtacggaa cggacgagct
     1801 tcccgaggag ctcctcgtgc agcgccttcc ggtgggcccg agcgggggcg tcctggcgcc
     1861 gccggacacg gccttcgcga cgccttcgga cgaagatctg ccgtctccgg accccgagct
     1921 cctcgccggg gcctggatgc ggggactcag gcttctcgag tcgcgcatcc ggcggaagct
     1981 ccagccccac gaggaccgca tgcgccggga gctgaatcgc gagatgcgga cgctcagcat
     2041 ccattaccgc tccttgatcg ccgaggagcg gaccgggcgg cgaaagcgtg gggaagggcg
     2101 cgaagccggg cgcatcttgc agctcaagga ggactgggag cggaagctct cggcggcggt
     2161 gcggcagcgg gtgctcgata cggaggccag gctcgtcgcc gtcgccctcc tctcggtcgt
     2221 ctcggacgga aaggccccgc gcgccgagcc gtcagcgagg cccgcgcggg cccgccggcg
     2281 cgtttgacgc ggcccgccgg aacgcgtaaa atcctcggct cacccgttca ggagggaatc
     2341 gtttgtcgac ccatctcgtg aagcatcaaa agatcacgaa gcggcagatc aaggaagacc
     2401 cgctcgtgac cgccgccttc aaggccaccg cccactggga gcgtcatggc agccgaatcc
     2461 tcgtggggct cggcgtggtc gccctcctcg cgctccttgg cttcttcatg atgcaggccc
     2521 ggtccaaggc cgaagagaag gcctccggag acctgttccg cgcgaatctg gcggcgatgc
     2581 agggggatta cgtgagcgcc gctccgatcc tcaaggagat cgtggacaac gagcccggaa
     2641 cggccgccgc gcgcgacgcc atgctcctcc tgggggacag cttcgcgtcg caggggaagc
     2701 cgggcgacgc cgcgacgtgg taccagaagt acatcgacaa ggccggcagc aaggaccgcc
     2761 agcggatcgg ctatctcgcg ctcgcgtccg cgctcgagga tgcggggcag ttcggcccgg
     2821 ccgccgatgc gttcgcgtcg tccgcggagc gctccgacac ggacaatctc cgtggccgcg
     2881 cgatgctggg ccaggcgcgc tgcctcgcgc gcgcgggtca gacgccgaag gcgatcgaga
     2941 tctacaagaa ggtctcgctc ctgccgcagg cggaagtgac gatcacgaac gccgccgacg
     3001 tccgcctggg cgagctcgcc gctccgaccg caccctagct tcgcggtttc gtgccgcgtc
     3061 cgatcttgct cgcgctggtg tggcatctcc accagccgag ctatcacgac gcggagaccg
     3121 gcgccgacct ccttccctgg acgcggctcc acgcgaccaa ggactaccgt gacatggtgc
     3181 gcgcgctcgc gcggacgccg cgcgtgcgcg tcacgttcaa cctgaccccg gtcctgctcg
     3241 accagctcga cgcgatcggg gccgggcgct ccgacccgta cctcgacgta gcgcgagcat
     3301 ccgccgcctc gctcaccgca gcgcaagcct ccttcctcct ggcccgcttc ttcgacgtga
     3361 atcacgagcg catgctgaac cccttcccgc ggtatcgaga gcttttcgag cggaaggaag
     3421 ccgggggtgc gttcaccgcg gacgatctcc gggaccttca aacatggttc catctcgcc
//
LOCUS       DASTKC010000253         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_693415, whole genome shotgun sequence.
ACCESSION   DASTKC010000253 DASTKC010000000
VERSION     DASTKC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693415"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFR25_10665"
     CDS             complement(<1..559)
                     /locus_tag="VFR25_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941548.1"
                     /translation="MSRLAQAIAGRRVSGVIALSPRSLAVALGEKPRSYLWIHLERKR
                     ARLAFADRLPTAPDPRGSPFGAIEEGLRGLLVRRATLDWDGLTLELGSEGASTHRLSV
                     TAERTRVNLIVSGGTEDGVLWAFHRDAEEGTGEAPPGFRAPQVEVADDPAAEQTLADD
                     IAAAFTEDFRRYIEKELHAAARVLAR"
     gene            complement(556..>2025)
                     /locus_tag="VFR25_10670"
     CDS             complement(556..>2025)
                     /locus_tag="VFR25_10670"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9941549.1"
                     /translation="RALWKDDPRGYHAQNLVWYVLACLALWLLAWDTSGRPLAATAGA
                     VLFALHPTHPESAAFISGRTDLIAGTLLFAALWTASRFGPGIRSPWMKLAPAALVLLP
                     GLFAKEVALFGAPVLLAALWVKDRELTVSDLARAAVPLALVACMYWIARVAVLGPAHL
                     PAVPPVQGTVPQILTSVAIVAIYVGLLFVPLGLSARHDIREVPGADFIFIAGLVVLGA
                     LTAGIARAARMRSPWLLPLSLFAATLLPICWVRILAGALVAERFLFVPGGALALGVAL
                     LPGLWGRSRDAGGEFLAACAAVAIWFLVLLLPRVAIWKDEPTLFTSILHDSPESAHVH
                     GLLGNYYYSHRDLPRAAEHFRRAHAIMPASGEMLLNLGAAEDEMGERDSALVHVRELN
                     ALQPDYAPGWYALGNLHVRVDRPDSAVAAYRMAIRLDPKFAQAENNLGVVLERMGRYD
                     EALEHYRRAGEVEPGNPEATRNQARLTALVDSLATKARR"
BASE COUNT          330 a          703 c          740 g          252 t
ORIGIN      
        1 gccgcgcaag gactcgcgcg gcggcgtgga gctccttttc aatgtatcgg cgaaagtcct
       61 cggtgaacgc cgcggcgatg tcgtccgcga gcgtctgttc cgcggccggg tcgtccgcga
      121 cctcgacctg cggcgcgcgg aatccgggcg gcgcttcacc cgtaccttcc tcggcgtcgc
      181 gatggaacgc ccagagcact ccgtcttctg ttccgcccga gacgatgaga ttcacccgcg
      241 tccgctccgc ggtcaccgag agccggtgcg tgctcgcgcc ctcggagccc agctcgagcg
      301 tgaggccgtc ccagtcgagc gtcgcgcgtc gcacgaggag gccgcggagc ccttcttcga
      361 tcgccccgaa cggggacccg cgcggatccg gcgccgtcgg gagccgatcg gcgaacgcga
      421 gccgcgcgcg ctttcgctcg aggtggatcc agaggtagga gcgcggcttc tctccgagcg
      481 cgaccgcgag cgagcgcggg gagagcgcga tcaccccgga gaccctgcgg ccggcgatgg
      541 cttgcgcgag acggctcatc gccgcgcctt cgttgccagc gaatcgacga gggcggtgag
      601 acgagcctgg ttgcgcgtcg cttccggatt gccgggctcg acctcgcccg cccggcgata
      661 gtgctcgagc gcctcgtcgt agcgccccat tcgctcgagc acgacgccga gattgttctc
      721 cgcttgcgcg aacttgggat cgaggcggat cgccatccga tacgcggcca ccgcggaatc
      781 gggccggtcc acgcgaacgt gcagattccc gagggcgtac cagcccggcg cgtagtcggg
      841 ctgcaacgcg ttgagctcgc gcacgtggac gagggcggaa tcgcgctctc ccatctcgtc
      901 ctcggccgcg ccgagattga ggagcatctc gcccgacgcg ggcatgatcg cgtgcgcgcg
      961 gcggaagtgc tcggcggcgc gcgggagatc gcggtggctg tagtagtagt tcccgaggag
     1021 cccgtgcacg tgcgccgact cgggtgagtc atggaggatc gacgtgaaga gcgtcggctc
     1081 gtccttccag atcgcgaccc gcggcaggag gaggacgagg aaccagatcg cgacggcggc
     1141 gcatgcggcg aggaactcac ccccggcgtc gcgcgaccgt ccccacaagc cgggcaggag
     1201 cgcgactccg agggcgagcg ctccccctgg gacgaacagg aaccgctccg ccacgagcgc
     1261 ccccgcgagg atccgcaccc agcagatcgg gaggagcgtc gcggcgaaga gcgacaacgg
     1321 gaggagccac ggcgatcgca tcctcgccgc gcgcgcgatc ccggcggtga gcgcgcccag
     1381 caccaccagc cccgcgatga agatgaagtc ggcccccggc acttcgcgga tatcgtgccg
     1441 cgccgagagg ccgaggggga cgaacaagag ccccacgtag atcgccacga tcgcgacgct
     1501 cgtgagaatc tgaggcaccg tgccctggac cggtggaacc gcggggagat gcgccggccc
     1561 gagcacggcg acgcgcgcga tccagtacat gcatgcgacg agcgcgaggg gaacggccgc
     1621 gcgcgcgaga tccgacacgg tcagctcgcg atccttgacc cagagggcag ccaggagcac
     1681 cggcgccccg aagagcgcca cttccttcgc gaagagcccg ggcaggagaa cgagcgccgc
     1741 gggcgcgagc ttcatccacg gcgatcggat cccgggcccg aatcgcgacg cggtccagag
     1801 cgcggcgaac aagagcgtgc ccgcgatcag atccgtgcgc cccgagatga aggcggcgct
     1861 ctcggggtgc gtcggatgga gcgcgaagag cacggccccc gcggtggccg cgagcggccg
     1921 gcccgaggtg tcccaggcaa ggagccagag cgcgaggcac gcgaggacgt accagaccag
     1981 gttctgcgcg tggtatccgc gcgggtcgtc cttccagagc gcgcg
//
LOCUS       DASTKC010000254         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_932218, whole genome shotgun sequence.
ACCESSION   DASTKC010000254 DASTKC010000000
VERSION     DASTKC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932218"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFR25_10675"
     CDS             complement(<1..563)
                     /locus_tag="VFR25_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9941550.1"
                     /translation="MRGCVDRHRVLVDRMSYAVRVHGSAGSPDTVVFLHGFAGSAEDW
                     RDAAEALAGSGYRTLAVDLPGHGSTDAPSDPSCYQSEATARDLATILTSLGVGAAHWV
                     GYSMGARVALRAALDAPSRVLSLTLESASTGIPDPAAREDRRRRDDELANDIEERGIA
                     WFVPHWESQPIFATQESLHQSRREAQRT"
     gene            complement(560..2344)
                     /gene="menD"
                     /locus_tag="VFR25_10680"
     CDS             complement(560..2344)
                     /gene="menD"
                     /locus_tag="VFR25_10680"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET9941551.1"
                     /translation="MAEIRSVGAAKQSATALLNLEWAERLVASLAHAGVRDAIVCAGS
                     RSAPLALAVDRSPLRAHVALDERAGAYYALGLAKASGRPVVVVTTSGTAAANLHPAVV
                     EAHHARVPLILLTADRPPELRDCGAAQTIDQIRLFGPAVRWFHEVGAPDGDPALAPYV
                     EGLARRAVLSAIGPPAGPVHLNLAFREPLVSDPESLPPGRTQGLEGEAALPDPKAPPP
                     GAIQRVARLLRTKRRGLILCGPDAAAGLADSVARLAAVTGYPILADPASQVRYGPHDR
                     TRVLGAYDGFLRAPAFAGRHAPEVMLQFGAPLTSKAFHLYAARHTDAIRVIVDDGGVP
                     RDPAHRATEIVAAGAAPAADALAEALAKGADPLPSWSEPFLRAEAAARDAVARHIART
                     PGISEGSAVASLAEAAPAETLLYMGNSMPIRDLDLFVPVSPKSLRVLANRGANGIDGV
                     LSSALGASAAGPVPVLAVVGDLSFHHDLNGLVAARTRGVAATIVVVNNDGGGIFSFLP
                     IARHGDAFERLVATPHGLRFEHAAALYGLPYEAPASLDELASSARDALERRETRILEI
                     ASDRARNRADHEALWAEVIAAVDGRAAP"
     gene            complement(2337..>2583)
                     /locus_tag="VFR25_10685"
     CDS             complement(2337..>2583)
                     /locus_tag="VFR25_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HET9941552.1"
                     /translation="DAALELIEAWEPRDRGWYAGPIGWIGANGDGEFTLGLRSALLDG
                     RRALLYAGAGIVLGSDPDREWEECEAKMRFVAEVLRG"
BASE COUNT          384 a          916 c          958 g          325 t
ORIGIN      
        1 gtgcgctgcg cctcacgacg gctctggtga agagattcct gcgtggcgaa gattggctga
       61 ctctcccagt ggggcacgaa ccatgcgatg ccgcgctcct cgatgtcgtt cgcgagctcg
      121 tcgtccctcc ggcgccggtc ctcgcgcgcg gcgggatcgg gaattccggt cgacgcgctc
      181 tcgagcgtga gcgagagcac tcgcgacggc gcatcgaggg cggcgcgaag cgccacccga
      241 gcccccatcg aatagccgac ccaatgcgcg gcgcccaccc cgagcgacgt caatatcgtc
      301 gcgaggtcgc gggccgtggc ctcggactgg tagcacgagg gatcggatgg agcgtccgtg
      361 cttccatgtc ccggcagatc cacggcgagc gtgcggtacc cggatcccgc gagcgcctcc
      421 gccgcatcgc gccagtcctc ggcggagccc gcaaagccat ggaggaagac gacggtgtcc
      481 gggctcccgg cgctgccatg cacgcgcacc gcgtagctca tgcgatccac gaggacgcgg
      541 tgccggtcga cgcagcccct catggcgccg ctctcccgtc gaccgcggcg atcacctcgg
      601 cccagagcgc ttcgtggtcg gcgcgattcc gggcgcgatc ggatgcgatc tcgaggatgc
      661 gcgtctcgcg ccgctcgagt gcgtcccgcg cggaggacgc gagctcgtcc aaggacgccg
      721 gcgcctcgta cggaagtccg tagagcgcgg ccgcgtgctc gaaccggagc ccgtgcgggg
      781 tcgcgaccag ccgctcgaac gcgtcgccgt ggcgcgcgat cggaaggaag gagaagatcc
      841 cgccgccgtc gttgttcacg acgacgatcg tcgcggcgac gccgcgcgtc cgcgcggcga
      901 cgagtccgtt gagatcgtga tgaaaggaga gatcgcccac caccgcgagc acggggactg
      961 gtccggccgc gctcgctccg agcgcgctcg agagcacgcc gtcgatgccg ttcgcgccgc
     1021 ggttcgcgag cacgcggaga ctcttgggcg agacggggac gaagagatcg aggtcccgga
     1081 tcggcatgct gttccccata tagaggaggg tctccgcggg agcggcctcg gcgagcgagg
     1141 cgaccgcgct gccttccgag attccgggcg tccgtgcgat gtgccgcgcc acggcgtcgc
     1201 gcgccgcggc ttcggcgcgg aggaagggct cgctccacga gggaagggga tcggctcctt
     1261 tggcgagcgc ttccgcgagc gcgtcggccg cgggcgcggc gccggccgcc acgatctccg
     1321 tcgcgcgatg cgcgggatcg cgcggcacgc cgccgtcgtc gacgatcacg cgaatcgcgt
     1381 ccgtgtggcg cgccgcgtag agatggaacg ccttcgacgt gagcggcgcg ccgaactgca
     1441 gcatcacttc gggcgcgtga cgacccgcga acgcgggagc ccgcaggaag ccgtcgtagg
     1501 cgccgagcac gcgcgtgcga tcgtgcgggc cgtaccgcac ctgggacgcc ggatcggcga
     1561 ggatgggata gcccgtcacc gcggcgagcc gcgcgacgga gtcggcgagg ccggccgccg
     1621 cgtcgggtcc gcacagaatg agcccgcggc gcttcgtgcg gaggagcctc gccacgcgct
     1681 ggatcgcgcc gggcggggga gccttagggt cgggcagcgc cgcctcgccc tcgaggcctt
     1741 gggtcctgcc gggcggcagt gactccgggt cggagaccaa gggctcgcga aacgcgagat
     1801 tgagatgcac cggcccggct ggcggtccga tggcggaaag gaccgcgcgc cgcgcgagcc
     1861 cctccacgta cggcgcgagc gccggatcgc cgtccggcgc gccgacttcg tggaaccagc
     1921 gcacggccgg gccgaagaga cggatctgat cgatggtctg agccgcgccg cagtcccgaa
     1981 gctcgggcgg gcgatccgcg gtgaggagga tcaagggaac gcgcgcgtga tgcgcctcga
     2041 cgaccgcagg atggagattc gccgcggcgg tgccggacgt cgtcacgacg acgacggggc
     2101 gccccgacgc cttggccagg cccagcgcgt agtaggcgcc ggctcgctcg tccagcgcga
     2161 cgtgcgcgcg gagcggcgag cgatccacgg cgagcgcgag cggagcggag cgggagcccg
     2221 cgcagacgat cgcgtcgcgc acgcccgcgt gcgcgagcga cgcaaccagt cgctccgccc
     2281 actcgagatt gaggagcgcc gtcgcgctct gcttcgcggc cccgaccgat cggatctcag
     2341 ccacgcagaa cctccgcgac gaagcgcatc ttcgcctcgc actcctccca ctcgcggtcc
     2401 ggatccgagc caaggacgat gccggcgccg gcgtatagga gcgcgcgccg gccgtcgaga
     2461 agcgccgagc gaagtccgag cgtgaactcc ccgtcgccat tcgcaccgat ccatccgatg
     2521 ggcccggcgt accagccgcg atcgcgcggc tcccacgctt cgatcaactc gagcgccgcg
     2581 tcg
//
LOCUS       DASTKC010000255         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2116442, whole genome shotgun sequence.
ACCESSION   DASTKC010000255 DASTKC010000000
VERSION     DASTKC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116442"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..695
                     /locus_tag="VFR25_10690"
     CDS             138..695
                     /locus_tag="VFR25_10690"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET9941553.1"
                     /translation="MKIKPVHWIVIGAVVAVVLSVWAWYAIGPGRSTKIAPERLTPLA
                     SELEGIRGVYLYYGVPGTDSLAAEYRDVVVKDRPADQVRAIYRELIAGPTGRYESPFP
                     QGAELLNAYYTSRGALYLDWNRTLAQAFRGGSGRERQLLGSIVLTAAANLPDVKQVAI
                     LIEGNPVETVGGHYDVMTPLQVAEW"
     gene            689..1591
                     /gene="murI"
                     /locus_tag="VFR25_10695"
     CDS             689..1591
                     /gene="murI"
                     /locus_tag="VFR25_10695"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9941554.1"
                     /translation="MVTKRRSKPIARRRPLGVFDSGIGGLTVVRHLLRLLPERRIVYF
                     GDTARVPYGAKSDATVRRFGEEAVKFLSHYDVDAIVVACNTVSAVALRPLQREFRSLP
                     ITGVIEPGAEAAVRETKSGRIGVIGTRATVASGAYVQAIHAAARRAARRVRVDAVACP
                     LLVPLAEEGMENSRAAREVLRDYLAPLQRRGIDTLILGCTHYPPFRRALQSVLGTRVK
                     LVDSGEATARQMARQAREAARAAGRTVKGTGRRGTLECFVSDIPLQFERIGRRFLGRS
                     MGKVGLIPQDDLPWFERNPIQGGR"
     gene            1602..>2048
                     /gene="rph"
                     /locus_tag="VFR25_10700"
     CDS             1602..>2048
                     /gene="rph"
                     /locus_tag="VFR25_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET9941555.1"
                     /translation="MKRSTSKHSAAAPAVKRVDGRKPADLRPLTIHRGYLKNAEGSAL
                     IEMGGTRVLCAATIEERVPPFLRNSGRGWVTAEYSMLPRSSHERIAREVNRGHVGGRT
                     HEIQRLIGRSLRAITRMEALGERQILIDCDVIEADGGTRTASITGSF"
BASE COUNT          315 a          682 c          723 g          328 t
ORIGIN      
        1 gcatgcccgg cgctcttcct cgaatctgcg ccggcggcgc gctcggggcc ggaggccatg
       61 agccgcggct acaccatcta cgattacacc cgcgtcgtgt cggacgcgat cgagcgggtc
      121 gtgcggatga agcgcggatg aagatcaagc ccgtccactg gatcgtcatt ggcgcggtgg
      181 tcgcggtcgt gctcagcgtc tgggcgtggt acgccatcgg cccgggccgg agcacgaaga
      241 tcgctcccga gcgcctgacg ccgctcgcgt cggagctcga ggggatccgg ggcgtgtatc
      301 tttattatgg tgttccgggg accgacagcc tcgcggccga gtatcgcgat gtcgtcgtga
      361 aggatcgccc cgccgatcag gtgcgcgcga tctaccgcga gctgatcgcg ggcccgaccg
      421 gtcgttacga atcgccgttc ccgcaagggg cggagctcct gaatgcgtac tacacgtcgc
      481 gcggcgcgct ctatctcgac tggaatcgca cgctcgcgca ggcctttcgg ggtggaagcg
      541 gccgcgagcg acagctcctc ggatcgatcg tgctcacggc ggcggcgaat cttccggacg
      601 tgaagcaggt agcgatcctg atcgagggca acccggtgga gaccgtggga ggacactacg
      661 acgtgatgac cccgctccag gtggcggaat ggtgacgaag cggcggtcca agcccattgc
      721 ccgtcgacgc ccgctcggtg tcttcgattc gggcatcggc ggactcacgg tggtgcgcca
      781 tctgcttcgc ctccttcccg agcgacggat cgtgtacttc ggcgatacgg ctcgcgttcc
      841 ctatggcgcg aagtccgatg cgacggtgcg ccggttcgga gaggaagcgg tgaagttcct
      901 atcgcactac gacgtcgatg cgatcgtcgt ggcgtgcaac accgtctcag cggtggcgct
      961 tcgtccgctc cagcgcgagt tccgctctct tcccatcacc ggcgtcatcg agccgggggc
     1021 cgaagcagct gtgcgggaga cgaagagcgg gcgcatcggc gtgatcggaa cgcgcgcgac
     1081 ggtcgcgagc ggcgcgtacg tgcaggcgat ccatgcggct gcccggcgcg cggccagaag
     1141 ggtgcgggtc gacgcggtcg cctgcccgct cctcgttccg ctcgcggagg aagggatgga
     1201 gaactcgcgc gcagcgcgcg aggtgctccg tgactacctg gcgccgcttc agaggcgcgg
     1261 catcgacacg ctcatcctcg gttgcacgca ctatccgccg ttccgccgcg cgcttcagag
     1321 cgtgctcggg acgcgcgtga agctcgtcga ctccggcgag gccacggcgc gccagatggc
     1381 gcgccaggcg agggaggcgg ctcgggccgc ggggcgcacc gtcaaaggca cgggacgccg
     1441 gggcacgctc gaatgcttcg tctcggacat cccgctccag ttcgagcgca tcggccggcg
     1501 gttcctgggc cgctcgatgg gcaaggtcgg gctcatcccg caggacgatc tcccctggtt
     1561 cgagcgcaat cccatccaag gagggcgatg acgtgacgcc tgtgaagcgc tcgacgtcca
     1621 agcacagcgc tgcggctccg gccgtcaagc gcgtcgacgg cagaaagccc gcggatcttc
     1681 gtccgctcac gatccatcgc ggttatctca agaacgccga gggctccgcg ctcatcgaga
     1741 tgggcggcac gcgcgtcctc tgcgcggcca cgatcgagga gcgcgttcca ccgttcctcc
     1801 gcaactcggg gcgcggctgg gtcaccgccg agtacagcat gctaccgcgc tcctcgcacg
     1861 agcggattgc ccgcgaggtg aaccgcggcc acgtgggcgg gcgcacgcac gagatccagc
     1921 ggctcatcgg ccgctcgctc cgtgcgatca cgcggatgga agcgctcggc gagcgtcaga
     1981 tcctgatcga ttgcgacgtg atcgaagccg acggcggaac gcgcacggcc tcgatcacgg
     2041 gatcgttc
//
LOCUS       DASTKC010000256         5947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_695179, whole genome shotgun sequence.
ACCESSION   DASTKC010000256 DASTKC010000000
VERSION     DASTKC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695179"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2485
                     /locus_tag="VFR25_10705"
     CDS             <1..2485
                     /locus_tag="VFR25_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941556.1"
                     /translation="ETLDIALQIASGMEAAHEQGVIHRDLKPGNVMITHGDQVKIVDF
                     GLAKGRLDAAQGEHSPTAHPAGTISSPTMTSPTIASPVDAGTIPGVILGTAPYLSPEQ
                     ARGKPVDRRTDIWAFGCIVYECLTGERAFQGETVSDTIARILEREPDWTRLPKSTPPR
                     LKDLLHRALEKDPRRRLRDMGEARLAIDEIRSGRAAPAASTPAKTERKLRFDRHTLML
                     AFGIGLTLGAALALNMTGFLGGKHVAHLNGPAWASFGVPPQVRVLGYEMTTDGRYLVI
                     LGAPKPTAGQEPPPQRLYARRMDSADFKPIDGSEGAEGFVLTYDGHYSFIAPASARSS
                     LRKIFYGPIDGSAPPVPIRDADPSRIQATFLESGRVLASVAGKQWIIYAMKREDVTAP
                     RPFDPRDSTKTYIVISSLPRDRGALLRVLSYDKGAFRQDIGVLDLKSGTTKIVIRDGG
                     SPHYASTGHLLFTRGGTLLAVPFDLGKLEVRGEPVAIMGGLRSNSNWTHAAFDMAGNG
                     TLLFARGGAGIQGRHAIILNDDGSTAEWSGERQPFERKLTVSPDGKRFASVITNSEAI
                     YEIWVSTRGNPTSQRLIAVPGADCDQPIWSPDGRHIAYTQISDSPNDGVYDYPLAGGQ
                     PRFLCGREAPDQGIGLASWQPDGSQILCVLTSGTTSSLAVVDVPPPGARPRTMRHLNL
                     AGGSVRAGAFSDDGRKLAYVQITESGKSDLFVCNYQKDGTLGAPYQVSRGGARVFVWT
                     PDGRNLLYANDQGQGMLAFAPGPDGTVRPPIVTFPDLQKYRFVQDLGDKLPDGRVLLV
                     QRGDDEDDVTNFDVVFNFFDVLKEKMKRN"
     gene            2608..4602
                     /locus_tag="VFR25_10710"
     CDS             2608..4602
                     /locus_tag="VFR25_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941557.1"
                     /translation="MTRHALLPAGALLCLLALSTAPLVASALADGSVEIQEVDENGNP
                     IGKDAAPQANQGSVQIQELDDQGRAVAPPRVTVPMEASHDPIRMTDETGQAANRQPSA
                     YLAPSATVAPASATIKTVIQPQPSHVVRFDDTIRDNATEMLDEGRQSFRYDTFGSERF
                     WGGDLRLHEAIEGDKHGGVGPGLTLKGALAMGLKIDSEALDPEMRDAIASGRVDLDSP
                     DIMLDLLRSKAIVGLSGNADGKGGLVSVGMQCALCHSTVDNSVADGVGRRLDGWANRD
                     LDMGALMAMAPTLMPLAEILLTDETTLRTALKTWGPGKFDPALVLDGKLERPDKKPAA
                     VLIPPTFGLAGLTRTASSGLGSVGHWASFASPRAGGSIRDPRLDDATQFPVAAQARMG
                     HLAGDADGAIAAKLPALEFYQLSIPAPSPPAGSFDEAAARRGAEVFRTEGRCVSCHAE
                     GLTSEPGYNFHKGDEIGIDNFQAERTPERLYRTKPLRGLWSHAKGGFYHDGRFATLTD
                     VVNHYDTVMALGLNDPQKSDLAAYLQSLGGEAEPAVASADALNGATNLSSGGRVETAA
                     WRITVGPSPALRGIPIRVSLQGIAAGGAPTDLETQVLDSGGRPVTTLGPWRFEPARAE
                     AMLVWNGKDAKGRPVTAGDYTVRVTAPSAGYKLDRVVVLR"
     gene            4696..>5947
                     /locus_tag="VFR25_10715"
     CDS             4696..>5947
                     /locus_tag="VFR25_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9941558.1"
                     /translation="MAWDASSRIDPTSYRQVTRASRIFGLLVALQGAVALAGWFTGIE
                     LLKGAFALGINIKTNTAIGLLLLGLALVLLGPPRRRGWRTWIGRLLAVVAIVLGLLTL
                     YEHVTGIDLGIDQALFTEAPGVPATSSPNRMGPPASVILPLIGLSLLLSDRRSPAGVR
                     LSQFAAMLALLVAAVPLLGYVYGVRELFGLARFTGIALTTAICFILIALGVLFARADR
                     GAMRLMVADDAGGMLLRRMIPAAIIIPVALGWARVALQDMGLYDANFGRTLLTVSFMV
                     VFSALIWWNAALISRVARGRDRAEDAERELTARLRIALDGERAAREAAEHANRMKDEF
                     LATLSHELRTPLNAILGWATLLAEESLSEEDVKRGLSTIQRNARLQARLIEDLLDMSR
                     IVSGKMRLEFSTVDLCLLVDATISA"
BASE COUNT          935 a         2106 c         1976 g          930 t
ORIGIN      
        1 cgagaccctc gacattgcgc tccagatcgc ctccggcatg gaggctgcgc acgagcaggg
       61 cgtcatccat cgcgacctga agccggggaa cgtgatgatc acgcacgggg atcaggtgaa
      121 gatcgtcgac ttcggcctgg ccaagggacg gctcgacgcg gcccaggggg agcattcgcc
      181 cacggcccac ccggccggca ccatctcctc cccgaccatg acgtcgccca cgatcgcttc
      241 cccggtggac gcgggaacga tcccgggcgt catcctcggc accgccccgt atctctcgcc
      301 cgagcaggcg cgcggcaagc cggtcgaccg ccgcaccgac atctgggcgt tcggctgcat
      361 cgtgtacgag tgcctcacgg gagagcgggc gttccaaggc gagaccgtct ccgacacgat
      421 cgcccgcatc ctcgagcgcg agcccgactg gacgcgactt cccaagtcga ctccaccgcg
      481 gctcaaggac ctcctccatc gcgcgctcga gaaggatccg aggcggcggc ttcgcgacat
      541 gggggaggcg aggctcgcca tcgacgagat ccggtcgggg cgcgcggcgc cggccgcatc
      601 gacccccgcg aagaccgagc gcaagctccg cttcgaccgg cacacgctga tgctcgcgtt
      661 cggcatcggc ctcacgctcg gcgcggcgct cgcgctcaac atgaccggct tcctcggcgg
      721 gaagcacgtg gcgcatttga acggcccggc gtgggcatcc ttcggcgtgc cgccgcaggt
      781 gcgggtcctc ggttacgaga tgacgaccga cgggaggtac ctcgtcatcc tcggcgcccc
      841 gaagccgacg gcgggccagg agccgccacc gcaacggctt tacgcgcgcc gcatggatag
      901 cgcggacttc aaaccgatcg acggcagcga gggggccgaa ggcttcgtcc tgacctacga
      961 cggccactat tcgttcatcg ctcccgcttc ggcgcgctcg tccctgcgga agatcttcta
     1021 cggaccgatt gacggcagcg cgccccccgt cccgatcagg gacgccgatc cctcgcgtat
     1081 ccaggcgacg ttcctcgagt cggggcgcgt cctggcgtcg gtcgcgggga agcagtggat
     1141 catctatgcg atgaagcggg aggacgtcac ggcgccgcgt cccttcgacc cgcgcgattc
     1201 gaccaagacg tatatcgtga tttcgtccct tcctcgggat cgcggggcac tcctccgcgt
     1261 gctctcgtac gacaagggag ccttccggca ggacatcggc gtgctcgatc tgaagtccgg
     1321 aacgacgaag atcgtcataa gagacggggg gagcccccac tacgcgtcga cgggacacct
     1381 cctcttcacg cgagggggca ccctgctcgc cgttccgttc gacctcggca agctcgaagt
     1441 tcgcggcgag ccggtcgcga tcatgggagg actgcgctcg aattcgaact ggacccacgc
     1501 cgccttcgat atggccggga acggaacgct tctcttcgcg cgcgggggcg cggggatcca
     1561 gggccgccac gcgatcatat tgaacgacga cggttccacc gccgaatggt ccggcgagcg
     1621 tcagcccttc gagcgcaagc tgacggtgtc ccccgacggg aagcgcttcg catcggtcat
     1681 caccaactcg gaagcgatct acgagatctg ggtctcgacg cgcgggaacc ccacgtcgca
     1741 gcggttgatc gcggttcctg gcgccgactg cgaccagccc atttggtcgc cggacggccg
     1801 tcacatcgcc tacacccaga tctcggattc gcccaatgac ggtgtctacg actacccgct
     1861 cgctggggga caaccccggt tcctctgcgg ccgtgaagcg cccgaccaag gaatcggtct
     1921 cgcgtcgtgg caaccagacg gatcccagat cctgtgcgtg ctcaccagcg gcacgacgtc
     1981 gagcctggcc gtggtcgacg tgccccctcc gggcgccagg ccgaggacga tgaggcacct
     2041 caatttggca ggcgggagcg tgcgcgcagg agcgttctcg gatgacgggc ggaagctcgc
     2101 ctacgtccag atcacggagt cggggaagag cgatctcttc gtgtgcaact accagaagga
     2161 cgggaccctg ggcgccccgt accaagtctc ccgagggggc gcgagggtct tcgtgtggac
     2221 gccggacggg cggaacctgc tctacgccaa tgaccagggg cagggcatgc tcgccttcgc
     2281 gcccggtccc gacggcacgg tcaggccccc gatcgtgacg ttcccggacc tccagaagta
     2341 ccggttcgtc caggatctcg gggacaagct ccccgacggc cgcgtcctcc tcgtccagcg
     2401 tggcgacgac gaggacgacg tcacgaactt cgacgtggtc ttcaacttct tcgacgtgct
     2461 gaaagagaag atgaaacgca actagatgtt gtttcccggc accccgccgg gcgtatcctt
     2521 gaaggcgcag aagggctccg gcgccacccc ccacgcggaa ccctggtccc gaaccacatc
     2581 ttgccccggc aacgaggaga ccaggtcatg actcggcacg ccttgcttcc cgcgggagcg
     2641 ctgctttgct tgctcgcgct ttccaccgcc ccgctcgtcg cctccgcgct cgcggacggc
     2701 tccgtcgaga tccaggaagt cgacgagaac ggcaatccga tcggcaagga cgccgcacct
     2761 caggccaacc agggctccgt ccagatccag gagctcgacg accagggacg cgccgtcgca
     2821 ccgccgcgcg tcaccgttcc gatggaggcg tcgcacgatc cgatccgtat gacggacgag
     2881 acggggcagg cggcgaaccg tcagccaagc gcgtacctcg caccttcggc caccgtcgcg
     2941 ccggcctccg cgaccatcaa gaccgtcatc cagccccagc cgtcgcacgt cgtccgcttc
     3001 gacgacacga ttcgcgacaa cgcgaccgag atgctggacg agggacgtca gtcgttccgc
     3061 tacgacacct tcggcagcga gcgcttctgg ggcggcgacc ttcgcctgca cgaggcgatc
     3121 gagggggaca agcacggcgg cgtcggtccg ggtctcacct tgaagggcgc gctcgcgatg
     3181 gggctcaaga tcgattccga ggcgctcgat cccgagatgc gcgacgcgat cgcgtccgga
     3241 cgcgtggacc tcgactctcc ggacatcatg ctcgatctgc tccgctccaa ggcgatcgtg
     3301 gggctctcgg ggaacgccga cggcaagggc ggtctcgtct cggtcggaat gcagtgcgct
     3361 ctctgccact cgaccgtcga caactccgtc gcggacggcg tggggcggcg tctcgacgga
     3421 tgggcgaatc gcgatctcga catgggcgcg ctgatggcga tggcgccgac gctcatgccg
     3481 ctcgcggaga tcctcctcac cgacgaaacc acgcttcgca ccgcgttgaa gacgtggggg
     3541 cctgggaagt tcgatcctgc gctcgtgctg gacggcaagc tcgaacgtcc cgacaagaag
     3601 ccggccgcgg tcttgatccc gccgaccttc gggctcgcgg gactcacgcg caccgcgtcg
     3661 agcgggctcg gctcggtggg gcactgggcc tcattcgcgt cgccccgcgc ggggggctcg
     3721 atccgtgacc ccaggctcga cgacgcgacg cagttcccgg tcgcggcgca agcgaggatg
     3781 ggtcatctgg ccggcgatgc ggatggcgcg atcgcggcca agctcccggc gctcgagttc
     3841 taccagctct ccattccggc gccctctcca ccggcgggca gcttcgacga agcggcagcg
     3901 cgtcggggcg ccgaggtctt ccgcacggag gggcgctgcg tctcctgcca cgcggagggg
     3961 ctcacgtccg agcccggata caacttccac aaaggggacg agatcgggat cgacaacttc
     4021 caggcggagc gcacgccgga gcgtctctat cgcaccaagc cgctccgcgg cctctggtcg
     4081 cacgcgaagg ggggcttcta tcacgatggc cgcttcgcaa cgctcaccga cgtggtcaac
     4141 cactacgaca cggtgatggc gctcggcctg aacgacccgc agaagtcgga cctcgccgcc
     4201 tacctccagt cgctcggagg cgaggctgag cccgccgtcg cgagcgccga cgccttgaac
     4261 ggcgccacga atctctcgag cggcggccgt gtcgagaccg cggcgtggcg gatcacggtc
     4321 ggtccgtccc cggcgcttcg cggaatcccc atccgcgtct cactccaggg gatcgccgcg
     4381 ggaggcgcgc cgaccgacct cgagacgcag gtgctcgact cgggcggccg gccggtcacg
     4441 acgctcggtc cgtggcgctt cgagcccgcg cgggcggagg cgatgctggt ctggaacggc
     4501 aaggacgcca aagggcgtcc ggtcacggcg ggggactaca cggtgcgggt cacggcgccg
     4561 tcggccggat acaagctcga ccgggtagtc gtgctccgct aaccccactg ggtcggacag
     4621 gcctcccggc gtactgggca ccgggggcgg gcgcgtccgg gtgagtacgc gcccgccccg
     4681 atgctatcct caagtatggc ttgggacgcg tcgtcgcgca tcgatcccac ctcgtaccgg
     4741 caggtcaccc gcgcgagccg gatcttcggc ctcctcgtcg cgctccaggg tgccgtcgcc
     4801 ctcgcgggat ggttcacggg gatcgagctt ctcaaggggg cgttcgccct cggcatcaac
     4861 atcaagacca acacggcgat cgggctcctc ctgctcggtc tggcgctcgt tctcctcggc
     4921 ccgccccgcc gccgcggctg gcgcacctgg atcgggcgcc tgctggctgt cgtcgcgatc
     4981 gtgctcggcc tcctgaccct gtacgagcac gtcaccggga tcgatctcgg gatcgatcaa
     5041 gctctcttca ccgaagcgcc gggcgttccc gcgacctcga gccccaaccg gatgggtccc
     5101 ccggcatcgg tcatcctccc cttgatcgga ctctcccttc tcctgtccga ccgccgttcc
     5161 cctgccggag tccggctgag ccaattcgcg gcgatgctcg cgctcctggt cgcggccgtg
     5221 ccgctcctcg gctacgtcta cggcgttcgc gagctcttcg gcctggcccg cttcaccgga
     5281 atcgcgctga ccacggccat ctgcttcatc ctcatcgcgc tgggcgtgct cttcgcacgc
     5341 gcggaccgcg gagcgatgcg gctcatggtc gccgacgacg cgggcgggat gctccttcgc
     5401 cgcatgatcc cggccgcgat catcattccg gtcgcgctgg gctgggcgcg cgtggcgttg
     5461 caagacatgg ggctctacga cgcgaatttc ggccggacgc tcctcacggt gagcttcatg
     5521 gtcgtcttct cggcgctgat ctggtggaac gcggcgctga tctcacgcgt ggcgagggga
     5581 cgcgaccggg ccgaggatgc ggagcgcgag ctcacggcgc ggcttcggat cgcgctcgac
     5641 ggggagcgtg ctgcgcgcga ggccgccgag cacgcgaacc gcatgaagga cgagttcctc
     5701 gccacgttga gccacgagct ccgaacgccg ctcaacgcga tcctgggctg ggccacgttg
     5761 ctcgccgagg aatccctctc ggaggaggac gtgaagcgcg gcctcagcac catccagagg
     5821 aacgcgcggc tccaggcgag gctgatcgag gatctcctcg acatgtcgcg gatcgtctcg
     5881 gggaagatgc gcctcgagtt cagcaccgtc gacctgtgcc ttctcgtgga cgcgacgata
     5941 tcggcgt
//
LOCUS       DASTKC010000257        14709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_932876, whole genome shotgun sequence.
ACCESSION   DASTKC010000257 DASTKC010000000
VERSION     DASTKC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14709
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932876"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..3520)
                     /locus_tag="VFR25_10720"
     CDS             complement(383..3520)
                     /locus_tag="VFR25_10720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9941559.1"
                     /translation="MDGLVPKHRSDVLHRTNGSLPRPADASAHGEHKAQELGDLYFAA
                     DNFVVALEYYRRALDAESNGNGAGPKNTEALLRIGTQIVECLRHRGDLNEAVDALHDL
                     HKRLRPHVTREQAGRLSASLALLLFERGRYRAAQRSASLAYRLLRETTLNLDIGRTEM
                     IFGVIALRTGDWNAAREYFESAIATYRRAGYDAGMASAYNSLGLLHKNHCRFKEAARF
                     LEQALRIGERSGLFHDSATYVHNLGIVHEKMGEWDLAEDHYRRALQMHTEVGNAGGKA
                     RALFCMGNLRRKRRDFSGAENLLRQALAIALDRSFPRETVLAREGLGDLYADRDQLNE
                     ARAELETALALADQFAPDSDLTVEVVRRLGDVQLRMGEGEAAQRNGDRALAIARRLGD
                     RIEEACSLRLLGLVAVEHGDLPTGQDRLGEASRILLAIGKRFELARLNMAAGLSWRRR
                     ARKGKSRATLDDSVGYLRRAVAAYESLGLPAHAARALLELSRSELMRGLVDEAVLHLD
                     HASSVLSPEEDPVLAREIEEFRAEIEQGLIEGTSAQSNEFAAFEEIRSVLRGRDSEAA
                     VSEVLGITARRVSAARGCVAAPAPHGGIEVVATIGMSFRVAQDLLTELERRIGAARLA
                     SAPVVTSRAGADARFRDLPGTTHLSPRTSVAMMPLSLPSGLPGYLYLDRPDDGPLGAF
                     KQREINLIAVLANHVAVAILELQRQRLARENTALRGRLLGSANDHGIVTRSPELLEIL
                     SLLERVGPSDATILIEGETGSGKGLLTRAVHASSARATRPLIQVNCAALPEQLLESEL
                     FGHVQGAFTGAVREKVGLFVEANGGTLFLDEVDKTTITIQGKLLQVLDNREVRPVGGN
                     KSTKVDVRVVCATNVNLKQRIARGEFLEDLYYRLNDICFRVPPLRERPEDVPLLVEHY
                     VRRFSAEMGKEIRGVDHDLVATMAGLKWRGNVRELEKVVKRMVVLANEGEMLSLRLLP
                     RELMQAEEENADAPATLRAEVAKVERRLIASALERHNWNKVQAAKELSLSYPTLLQKI
                     KLFRLDRRLQARQRA"
     gene            complement(3607..3906)
                     /locus_tag="VFR25_10725"
     CDS             complement(3607..3906)
                     /locus_tag="VFR25_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941560.1"
                     /translation="MRVKVSRRLFGMCLLLTVLCLTPATLPTAYAKPIGWENLPDPNN
                     PQGPKGDGDGTVVKAKSMLPTSSVSSAKSTASGSSVWSLLRTYVQLVRMGYGSRL"
     gene            complement(4130..5083)
                     /locus_tag="VFR25_10730"
     CDS             complement(4130..5083)
                     /locus_tag="VFR25_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET9941561.1"
                     /translation="MRIGAVDIGTNSVRLLVADVDERERLRTAHRMGEISRLGEGIDR
                     TGLIDEAAAARTLECLERFVHEAEYSGASRIRVAGTNAFRVARNGPEIAARFADRIGY
                     PVEILTGEEEAQLVFLAVLSGQGSSPGKAVVVDIGGGSTEIITGEGERGTQVISLELG
                     CVRLTERLIHTDPPTQAELDSVKSHVLEVFADKLGQWGAAGLDRAIGVGGTVTAFGAL
                     DLGLVKYDPSRIENHHLTRDRIDAIAKHLCTIPLVQRRDLAGVSRGRADIIPAGAIIL
                     CEFVNRFAVNGVYVSTRGLRYGLVLSEARRAAGAGLKAAQA"
     gene            5246..6304
                     /gene="rlmN"
                     /locus_tag="VFR25_10735"
     CDS             5246..6304
                     /gene="rlmN"
                     /locus_tag="VFR25_10735"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9941562.1"
                     /translation="MKPMDEQEPLVGMSAPELERAMLSLGEKPFRGRQIADWMYLHGA
                     SSFSEMTNLPLPLRARLEEGFTLGTLTERARRETPDRKTRKVAYALPDGGLIESVYMS
                     TPRRYTFCLSSQHGCGFACRFCATGRMGRGRNLTTGEIVEQALRLRRELPPDQREFNI
                     VFMGMGEPLENYDAVMRALDIITDPRGLHVASKRITISTVGLVPQILRLASEGRRYRL
                     AISLHAATDELRSKLMPVNRTFPLEPLMRAVSTHIERLGERVTFEYILIEEINDSLAD
                     AAKLVRLVGQLPCRVNLIPYNPIGPGRFKRPSPERIQRFVDHVGPRVQAVTVRYSQGV
                     EIGAACGQLAGEKEIALR"
     gene            6320..7870
                     /locus_tag="VFR25_10740"
     CDS             6320..7870
                     /locus_tag="VFR25_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941563.1"
                     /translation="MLGTLRGRLLALFFLVALIPSLGLTLFVTQYLARSLATLRNPET
                     ERALSQSLEVIRGGLERLASDARQHAEVMALDPETIRLARLGRLQDLERFLRDEARNR
                     GIDYVCYYDMRDGTKRLFGARLGPRISLPDPEPSSLAQAVEHGGLITGDDAPRQVSGA
                     ASVDSVHVILAGYQLDPEISSEIVALQRNLSMYRRLGVYTWLSQRSLWIFAALWSLVL
                     GGVSFALAWAVSRSIARPIVALQSAMERVSQGDLGGGHVTPSGTREVRYLAGAFNRMI
                     GEIRASRRALLRAERLAAWREVARAVAHEIRNPLTPIQFALQRLREEAQRGEPPRADV
                     VAENADAILREVRSLQEFATAFSAVAQLPEPKFGPTDVVQLVEDAARLYRGSSPVEFR
                     VEVERPVPLAWADAGQIQRVLVNLIKNAVEVLGDKGQVILRVRRDLEANGVLLEVEDN
                     GPGMDPATVERAAHPGFTTKSSGSGLGLTLVTRIVEQHGGRFGLDSNPGAGTRAWVSL
                     AEAEGETA"
     gene            7867..9234
                     /locus_tag="VFR25_10745"
     CDS             7867..9234
                     /locus_tag="VFR25_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9941564.1"
                     /translation="MSASAAAGKTRVLIVDDEPSVLLEIAASLKRHYDPLTASNVDEA
                     EKILARERVGLLITDLKLPGRDGLALLESAKASQPDLPVVVMSGHGTIEEAVKAIQLG
                     ALDFVEKPFGPDRLQLTVDRALELRALRRENEELRALTGVSNEMVGKSAVLDQVRAEI
                     AKVARTDAKVLVTGESGSGKELVARAIHKASARARGPFEKLNCAALPKDLVESELFGY
                     EKGAFTGAVQMKRGRLEAADQGTLFLDEVGDMSLDTQAKFLRAIETGEIERLGSTRTI
                     AVDTRIVAATNKDLQAEIAAGRFREDLFYRLNVVPIHLPPLRQRREDIPLLIEHFAAK
                     LSVEHGRPQRRFLPDALERLSEYSWPGNVRELKNLVERILIMTEGDTVSLAEVEEVLP
                     QGQDDEPPSEIKAARDKAERDTILAMLKQCQWNVTEASRRLGMDRGYLHRKIRRYGLV
                     RESTA"
     gene            9218..10438
                     /locus_tag="VFR25_10750"
     CDS             9218..10438
                     /locus_tag="VFR25_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9941565.1"
                     /translation="MNRQPERLAAAFVLAAGAWMAWPLSSRAAVPDSLAAIIPVLSDS
                     TANHPSAPKAGFQQADWETLATVPDEARPALVYGFRYNRVDGPAPTLGGAVRSERFPN
                     PLVYAKFTYAFSRERFLYDVGGEAPLGDHARFRVGGAAYRRTETEDEWIVGETENTLY
                     ALFARTDYRDYYETSGYEGHVAWDPGTDFGARVEGRMEDQRSLENEANASLFGAHDRF
                     RPNPPIEDGQDGVLALGMRIGPAAIPPEGGTHGDVVYERAGDALHGDFDYGRVRAVVR
                     TRVRLSPKQDARARLVGGSTLDGTLPPQKLWYLGGISTLRGEDFKERQGDQFLLANAE
                     YYLLVRKNVWGFGFLDWGSAWFGRDNIDRQRFLLDGGLGVRIGQGPLALTAARNLQEA
                     HAAIHVLVRFSETF"
     gene            10440..11117
                     /locus_tag="VFR25_10755"
     CDS             10440..11117
                     /locus_tag="VFR25_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HET9941566.1"
                     /translation="MLAPGRVWVAAWVLALALVAITPRAIVADDLGLEVDPVEDVAGA
                     ICVSYRVAEPFTPRLEETLQRGMPATVSFEVGLWKRRALWFDRLVLAIRSDHKVVYDD
                     WRQAFRIRSGSNPPRDRSAPSFEALREVLFTQSRLPLAATSSLEPGATYYASVRVTIK
                     PVAPEDLGEIEDWLSGDKDSEQSTGLPDYLLGLAVSLSGLGERTEMEKGPKFVPARLS
                     AAPATTE"
     gene            complement(11140..12582)
                     /locus_tag="VFR25_10760"
     CDS             complement(11140..12582)
                     /locus_tag="VFR25_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941567.1"
                     /translation="MRAYRSSARTRVAPHSLSLLPWLLLLTGLVLAAGRAGGATPTPP
                     KPATPPDTTGGFRVRVSEEGDASSLKIRRQEERIRREVERAKREAARIKADLPKPPDV
                     PDAPDIPDVNIDLDSNDNNLVRFGEDIVIPADKVIDGDVVAIGGDVTVYGRVKGDCVS
                     VGGTVNVKDKGVVEGDAVSMGGGVATSDSASVGGSNVSLGSPWTHSHAFWPMVGLFGA
                     VGTGIWLVHTLVRLALTVFLAWLALLLARERMVYAVDTMYARFGRSFLWGLAGFAGSL
                     VALPTAIVLLILVGAIAIAILAITIIGIPVAIVLLLGLILGIIGLVLGVVVAVFLGFL
                     NGAMFLGQRVLGRTTERGKNPLLAIAVGVLLITTLKVAGKLIGVLGLIVFHPIAIALA
                     IATGALAAILTIAGFGAMMQTRFARGPHGEGLPTGMWWPRRRPSPAAAPAGAPGVVSE
                     APITGAASVATPPPPPASPPIVEGGTSDAP"
     gene            complement(12563..13201)
                     /locus_tag="VFR25_10765"
     CDS             complement(12563..13201)
                     /locus_tag="VFR25_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9941568.1"
                     /translation="MKTQLTCKACEPELSAYVDGALHPAVARVLEAHVTACPSCRVKL
                     DAYRAIAAGLAELPELEAPAWLEARIVARMTRPARLLRALRGGFATAGALSFALTIGL
                     VAFLPQLARQWGLPEPATWPVHVARGTLEAVVKFVRHLSLEVAFWEPIGRPIWSAIHA
                     LGTLPRVAWVALQTSEAQAALAVAITVGIALFFVLRPSRTREGGVGHACLSL"
     gene            complement(13198..13764)
                     /locus_tag="VFR25_10770"
     CDS             complement(13198..13764)
                     /locus_tag="VFR25_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9941569.1"
                     /translation="MGSDERDLVERCLARDERAYRELVRRYQGAVMNLAWKITGSADD
                     AAEVAQETFIRVLRSLGTYDPSRPFRTWLFKITSNLALDTIRRRKRRPIALEDLGDED
                     GPTIEPADTGPGPDEGYRIERSDARFESLVRELPEHYQAILFLRYKQELSYEDIAESM
                     NMPLGTVKVRIHRAHEMLRRKLTARGEG"
     gene            13972..>14709
                     /gene="eno"
                     /locus_tag="VFR25_10775"
     CDS             13972..>14709
                     /gene="eno"
                     /locus_tag="VFR25_10775"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420698.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9941570.1"
                     /translation="MTEIESVFAREILDSRGFPTLEAEVALVGGAIGRAAVPSGASTG
                     SREALELRDGDAKRYLGKGVAQAVRNVNEAIAPALVGENAADQAAIDKLLIELDGTPS
                     KKKLGANALLGVSIACAKAAADAHALPLYRYLGGANARTLPVPLMNVINGGAHADNNL
                     DIQEFMIVPVGFDSFADALRAGSEVFHTLKKLLHEKGLVTAVGDEGGFAPNLGANSEA
                     LDFLLRAIEKAGYRPGEQVALALDVAAS"
BASE COUNT         2328 a         5030 c         5093 g         2258 t
ORIGIN      
        1 atacttccga aaaaaattga gccccggcct tgccagctct cttcgggtta catcgcttcc
       61 tcagaggccg gtgctctgat tggcgactaa cccatgggct aacgctggta aggcccgcag
      121 gctctttgga ggtccccccc tccgatggac atgctattgc acccgccatg ccacctcgaa
      181 tagcgtcccc aaaaagtccg cgattcgccc gcaactcaat ggatcccagt ggattacaaa
      241 acggtgaacg accgtggtcc ggcgcgactt cgaggtccga atcgatggat ttatgcggtc
      301 ggtttaatag tcgggacgaa agcgatcgct aatcgatgta gggacaatcg attaggatta
      361 ttaaattgtt ttataaggaa atttaagccc tttgccgcgc ctggagcctg cggtccaggc
      421 ggaacagctt gatcttctgg aggagggtcg gataggagag cgacagctcc ttcgcggcct
      481 ggaccttgtt ccagttgtgc cgctcgagag cggaggcgat gagccggcgc tccaccttgg
      541 ccacctcggc ccggagggtg gccggggcgt cggcgttctc ctcttccgcc tgcatgagct
      601 cgcgcgggag gagccggagc gacagcatct caccttcgtt ggccagcacg accatccgct
      661 tcacgacctt ctccagctcg cgcacgttgc cgcgccactt gaggccggcc atcgtcgcga
      721 cgaggtcgtg gtccacgccc cggatctcct tgcccatctc ggcggagaag cgccgcacgt
      781 agtgctcgac caggagcgga acgtcctcgg gccgctcgcg cagcggcggc acgcggaagc
      841 agatgtcgtt gagccggtag tagaggtctt cgaggaactc gccacgcgcg atccgctgct
      901 tgagattgac gttggtcgcg cagaccacgc gcacatccac cttcgtcgac ttgttgccgc
      961 cgaccgggcg gacctcgcga ttatcgagca cttgaaggag cttgccctgg atcgtgatgg
     1021 tcgtcttgtc gacctcgtcc aggaagagcg tgccgccgtt cgcctcgacg aagaggccga
     1081 ccttctcgcg cacagcgccg gtgaacgcgc cctggacgtg tccgaagagc tccgattcga
     1141 ggagctgctc gggcagcgcc gcgcagttca cctggatgag cggcctcgtg gcgcgcgcgc
     1201 tcgacgcgtg caccgcgcga gtgaggaggc ccttgccgga gcctgtctct ccctcgatga
     1261 ggatcgtcgc gtcgctcgga cccacgcgct cgaggagcga gaggatctcg aggagctcgg
     1321 ggctccgcgt cacgatgccg tgatcgttgg cggatccgag gaggcggccg cggagcgccg
     1381 tgttctcgcg cgcgagacgc tggcgctgga gctcgaggat ggccacggcg acgtggttcg
     1441 cgagcaccgc gatgagattg atctcgcgct gcttgaacgc tccgagcggt ccgtcgtcgg
     1501 ggcgatcgag atagaggtag ccggggagtc ccgacgggag cgagagcggc atcatcgcga
     1561 cgctcgtccg cgggctcagg tgcgtcgtgc cgggcagatc gcggaagcgc gcgtccgctc
     1621 cggcgcggct cgtgaccacg ggggcggaag cgagccgggc cgcgccgatc cggcgctcca
     1681 gctcggtcaa gaggtcctgc gcgactcgga acgacatgcc gatcgtcgcg acgacctcga
     1741 tcccgccgtg cggcgcggga gccgcgacgc agccgcgcgc ggcggagacg cgccgcgcgg
     1801 tgatcccgag cacttcggag accgccgcct cggaatcgcg gcctcggagc acgctccgga
     1861 tctcctcgaa ggcggcgaat tcgttgctct gcgcggacgt cccttcgatc agtccctgct
     1921 cgatctcggc gcggaactcc tcgatctcgc gcgcgaggac cggatcttcc tcgggcgaga
     1981 gcacggagct cgcatgatcg agatgaagca ccgcctcgtc gacgagcccg cgcatgagct
     2041 cgctccgcga cagctcgagg agcgcgcgcg ccgcgtgggc cggaaggccg agactctcgt
     2101 aggcggcgac ggcgcggcgg aggtacccga ccgagtcgtc gagggtcgcg cgcgactttc
     2161 ccttgcgcgc gcggcggcgc caggagagcc cggccgccat gttgaggcgc gccagctcga
     2221 agcgcttgcc gatcgcaagg aggatgcggc tcgcctcgcc gaggcggtcc tggccggtgg
     2281 gcaggtcgcc gtgctcgacc gcgacgagac ccagaagcct gagcgagcag gcctcctcga
     2341 tccggtcgcc gagacggcgc gcgatggcga gcgcgcggtc accgttccgc tgcgcggcct
     2401 caccctcgcc catgcggagc tggacgtcgc ccaggcgtcg aacgacctcg acggtcagat
     2461 ccgaatcggg cgcgaattga tcggcgagcg cgagcgccgt ctcgagctct gcgcgagctt
     2521 cgttcagctg gtcgcggtcg gcgtagaggt caccgaggcc ttcgcgagcg agaaccgtct
     2581 cacgtgggaa gctccggtcg agggcgatgg cgagcgcctg gcggagcagg ttctcggcgc
     2641 cggagaagtc gcggcgcttc cgtcgcagat tgcccatgca gaagagggcg cgcgctttgc
     2701 cgcccgcgtt cccgacctcg gtgtgcatct gaagtgcgcg gcggtagtgg tcctcggcca
     2761 gatcccactc gcccatcttc tcgtggacga tgccgaggtt gtgcacgtac gtcgccgagt
     2821 cgtggaagag ccccgaccgc tcgccgattc gaagcgcctg ctcgaggaac cgcgccgcct
     2881 ccttgaagcg gcagtgattc ttgtggagta gcccgaggct gttgtacgcg gacgccatgc
     2941 ccgcgtcgta gccggcgcgg cggtacgtcg cgatggcgct ctcgaagtat tcgcgggccg
     3001 cattccagtc gcccgtccgg agcgcgatga ccccgaagat catctcggtc cgtccgatgt
     3061 ccagattgag cgtcgtctcg cgaagaagcc ggtaggcgag cgacgcggag cgctgtgcgg
     3121 cgcgatagcg accccgctcg aacaggagca gcgcgaggct cgcggacagg cgacccgcct
     3181 gctcccgcgt gacgtggggg cggagccgct tgtggagatc gtggagcgcg tcgaccgctt
     3241 cgttcaggtc gccgcgatgg cggaggcact cgacgatctg agtgccgatt cgcaggagag
     3301 cttcggtgtt cttggggccg gcgccgttcc cgttggactc cgcgtcgagt gcgcggcggt
     3361 agtactccag cgcaacgacg aagttgtcag cggcgaagta gagatcgccc agttcctggg
     3421 ccttgtgctc gccatgcgca ctcgcatcgg cggggcgcgg cagcgagccg ttagtccggt
     3481 gcaggacgtc ggatctatgc ttcgggacga gcccgtccat tccgaccctc gatgtaaggc
     3541 agaactacgg ggcgcggccc gaacggaggt cgggccgcgc acacccggtg cgcctagtcg
     3601 tgcccgttac aggcgcgagc cgtagcccat ccgcaccaac tgcacgtacg tcctcaacag
     3661 gctccacact gacgagccac tggccgtcga tttggccgaa gacacgctgc ttgtcggcag
     3721 catggacttc gccttcacga ctgtaccgtc gccatcaccc ttcggacctt gaggattgtt
     3781 cgggtcggga aggttttccc atccgatggg tttggcgtac gcggtgggca gagtggccgg
     3841 cgtcaggcag agcacggtca agaggaggca catgccgaag aggcggcgtg agactttgac
     3901 cctcattccc ttgctcctcg tcgaagcgac acccggcgca tcagccgctt cgcgttgtga
     3961 acccggcagt tcgccgaggg acgtctagcc gcggtggctg gaccgctggc cgagagggat
     4021 gtcctgggag gacaggcgtc ctttgacgat gactgccgct gttaacggag aggatacgaa
     4081 cccgccccgt aaacgtcaag cccaactttg ttttatagcg agattatttt caggcctggg
     4141 cggccttgag acccgctccg gccgcccggc gcgcctcgct cagaacgagg ccgtaccgca
     4201 atcctcgcgt ggatacgtag accccgttca cggcgaagcg gttcacgaat tcgcacaaga
     4261 tgatggcccc cgccgggatg atgtcggccc tgccgcggct gaccccggcc aggtcccgcc
     4321 gctgaacgag ggggatggtg cagaggtgct tcgcgatcgc gtcgatccgg tcgcgggtca
     4381 gatggtggtt ctcgatgcgg gaggggtcgt atttcacgag ccctaagtcc aaggcaccaa
     4441 aggcggtaac cgtcccgccc acgccgatgg cgcggtccag gccggcggcg ccccactgcc
     4501 ccagcttgtc cgcgaagacc tcgaggacat gggatttcac cgagtcgagc tcggcctggg
     4561 tcggggggtc ggtgtggatg aggcgctcgg tgaggcggac gcatcccaat tcgaggctga
     4621 tgacctgggt gccgcgctcc ccttcgccgg tgatgatctc ggtcgagccc ccgccgatgt
     4681 ccacgacgac ggccttgcca ggcgaagaac cctggccgct caaaacggcg aggaagacga
     4741 gctgggcctc ttcctcccca gtcaggatct caaccgggta gccgattcgg tcggcgaatc
     4801 gagccgcgat ctcgggaccg ttccgggcca cccggaaggc gttggttccg gcgacgcgga
     4861 tccggctggc gccggagtac tcggcttcgt ggacgaagcg ttcgaggcat tcgagggtgc
     4921 gggcggcggc ggcctcgtcg atgaggcccg tgcggtcgat gccctcgccc aggcgggaaa
     4981 tctcgcccat gcgatgcgcc gtgcgcaggc gctcgcgctc gtcgacgtcc gcgaccaaga
     5041 ggcggaccga attggtgccg atgtcgacgg cgccaattct caaagtggag tcgattggac
     5101 cggaaacggt cgttccccct gataggacgg ggccgcggcc cgcgcaggcc ccgcattggt
     5161 tgactcccaa cctaggggaa cgtagcatac gcaggcggtg tttgtaaaga cggaagccat
     5221 tatcagaacg gaaaatggag cgatcatgaa gcctatggac gagcaggagc cgctggtggg
     5281 gatgagcgcc ccggagctgg agcgggcgat gctctccctg ggagagaagc cgttccgcgg
     5341 tcgtcagatc gcggactgga tgtatctcca tggcgcttcg tcattttccg agatgaccaa
     5401 cctgccgttg ccgctccgcg cccgcctcga ggagggcttc acgctcggga ccctgaccga
     5461 gcgggcccgc cgcgagacgc cggaccgaaa gacccgcaag gtggcctatg ccctcccgga
     5521 tggaggtctg atcgaatcgg tctacatgtc caccccgcgc cgctacacct tctgcctctc
     5581 gtcgcagcac ggctgcgggt tcgcctgccg cttctgcgcc accgggcgca tggggcgggg
     5641 acgaaatctc acgacgggcg agatcgtgga gcaagccctt cggctgaggc gcgagctccc
     5701 gcccgaccag cgggaattca acatcgtgtt catggggatg ggcgagcccc tcgagaacta
     5761 cgacgcggtc atgcgcgcgc tcgacatcat caccgacccg cgcggcctcc atgtcgcatc
     5821 gaagcggatc accatctcca ccgtggggct cgtgccgcag atcctgcgtc tggcctccga
     5881 ggggaggcgc taccggctgg ccatctcgct ccacgcggcc acggacgagc tccgctccaa
     5941 gctcatgccg gtcaaccgaa cgttcccgct cgaacccttg atgcgcgcgg tgagcacgca
     6001 catcgagcgc ttgggcgagc gcgtcacgtt cgagtacatc ctgatcgagg agatcaacga
     6061 ctcgctcgcc gacgcggcca agctcgtgcg cctcgtcgga cagcttccct gccgcgtgaa
     6121 cctgatcccc tacaacccga tcggcccggg ccgcttcaag cgcccctcgc ccgagcggat
     6181 ccagcgcttc gtcgaccatg tcggcccgcg tgtccaggcc gtgaccgtgc gctacagcca
     6241 gggagtcgag atcggcgccg cctgcggcca gctcgccggc gagaaagaga tcgcgctccg
     6301 ctagcgcgga gcgagggcga tgctcggcac gctgcggggg cgcctcctcg ctctgttctt
     6361 cctcgtcgcg ctcattccct ccctcggcct cacgctgttc gtgacccagt acctcgcgcg
     6421 cagtctcgcg acgctccgga atcccgagac cgagcgcgcg ctctcgcagt cgctcgaggt
     6481 catccggggc ggactcgagc gcctcgcgag cgacgcgcgc cagcacgccg aggtgatggc
     6541 cctcgatccc gagacgattc gcctggcgcg cctgggccgg ctgcaggacc tggagcgctt
     6601 cctccgcgac gaggcccgga accgcggcat cgattacgtc tgctactacg acatgcggga
     6661 cggaacgaag cgtctcttcg gcgcgcgtct cgggccccgc atcagcctcc ccgatcccga
     6721 gccgtcgtcg ctggcgcagg ccgtcgagca cggcggcctc atcaccgggg acgacgcgcc
     6781 gcgccaggtg agcggggcgg cgtcggtcga ctcggtccac gtgatcctcg ccggatatca
     6841 gctcgacccc gagatctcct cggagatcgt cgcgcttcag cggaatctct ccatgtaccg
     6901 gagactcggg gtctacacct ggctctcgca gcggagcctc tggatcttcg ccgcgctctg
     6961 gagcctcgtc ctgggcgggg tgagcttcgc gctcgcgtgg gcggtgtcgc gctccatcgc
     7021 gcgtcccatc gtggcgctcc agtcggcgat ggagcgcgtc tcgcagggcg atctgggggg
     7081 cggacacgtg acaccgtccg gaacgcgcga ggtgcgctat ctggcgggcg cgttcaaccg
     7141 catgatcgga gagatccggg cgtcgcggcg cgcgctcttg cgcgccgagc ggctcgcggc
     7201 ctggcgcgag gtggcgcgcg ccgtcgcgca cgagatccgg aatccgctca cccccatcca
     7261 gttcgcactg cagcggctcc gagaggaagc gcagcgcggc gagccgccgc gcgcggacgt
     7321 ggtcgccgag aacgcggacg ccatcctccg cgaggtgcga tcgctccagg agttcgccac
     7381 cgcgttctcg gcggtggcac agctccccga gcccaagttc gggccgaccg acgtcgtcca
     7441 gctcgtggag gatgcggccc ggctctatcg aggatcgtct cccgtggaat tccgcgttga
     7501 ggtggagcga cccgttccct tagcctgggc cgacgcggga cagatccagc gcgttctggt
     7561 gaatctcatc aagaacgcgg tggaggtcct gggcgacaag gggcaggtga tcctccgggt
     7621 gcgtcgggac ctcgaagcga acggagtcct gctcgaagtc gaggacaacg gcccgggcat
     7681 ggatccggcc acggtggagc gcgcggccca ccccggcttc acgaccaagt ccagcgggag
     7741 cggccttgga ttgacgctcg tcacgcgcat cgtcgagcag cacggtggac ggttcggact
     7801 cgattcgaat cccggcgcgg gaacgcgcgc gtgggtgagc ttggcggagg cggagggcga
     7861 gacggcgtga gcgcgagcgc ggcagcgggc aagacgagag tgctcatcgt ggacgacgag
     7921 ccgtccgtgc tcctcgagat cgcggccagc ctgaagcgcc actacgatcc cctgaccgcc
     7981 tcgaacgtgg acgaggccga gaagatcctc gcgcgcgagc gggtcggcct cctcatcacc
     8041 gatctcaagc tacccggacg cgacgggctc gcgctcttgg agagcgccaa ggcgagccag
     8101 cccgatcttc ccgtcgtcgt gatgtcgggg cacggcacga tcgaggaagc ggtgaaggcg
     8161 atccagctcg gcgccctcga cttcgtcgag aagccgtttg ggccggaccg gctccagctc
     8221 acggtggacc gcgcgctcga gctgcgcgcg ctccgccgcg agaacgaaga gctccgcgcg
     8281 ctgaccggcg tgagcaacga gatggtcggc aagagcgcgg tgctcgatca ggtgcgcgcg
     8341 gagatcgcga aggtggcgcg caccgacgcg aaggtgctcg tgaccggcga gagcgggagc
     8401 ggcaaggagc tcgtcgcgcg cgccatccac aaggcgagcg cgcgtgcgcg cggccccttc
     8461 gagaagctca actgcgcggc gctccccaag gatctcgtgg agagcgagct cttcggctac
     8521 gagaagggtg cgttcacggg ggcggtccag atgaagcgcg ggcgcctcga ggcggcggac
     8581 caggggacgc tcttcctgga cgaggtcggg gacatgagcc tcgacacgca ggcgaagttc
     8641 ctccgcgcga tcgagacggg cgagatcgag cgcctgggct cgacccgcac gatcgcggtc
     8701 gacacgcgga tcgtcgccgc gacgaacaag gatctccagg cggagatcgc ggcgggccgg
     8761 ttccgcgagg atctcttcta ccggttgaac gtcgtgccga tccatctgcc gccgctgcgc
     8821 cagcggcggg aggacatccc gctcttgatc gagcacttcg cggccaagct ctccgtcgag
     8881 cacggccggc cacagcggcg cttcctgccc gatgcgctgg agcggctcag cgagtactcc
     8941 tggcccggca acgtgcgcga gctgaagaat ctcgtggagc ggatcctgat catgaccgag
     9001 ggcgataccg tgtccctggc ggaagtcgag gaggtgctgc ctcaggggca ggacgacgag
     9061 ccgccgagcg agatcaaggc cgcccgcgac aaggccgagc gcgacacgat cctcgcgatg
     9121 ctgaagcagt gccagtggaa cgtgacggag gcgtcgcgcc ggctcggcat ggaccgcggc
     9181 tacctccatc gaaagatccg acgctacggc ctggttcgtg aatcgacagc ctgagcgact
     9241 ggccgcggcc ttcgtgctcg cggcgggcgc gtggatggcg tggccgcttt cctcgcgcgc
     9301 ggcggtgccc gacagcctgg ccgcgatcat cccggtgctg tccgactcga ccgcgaacca
     9361 cccgagcgcg cccaaagccg ggttccagca ggcggactgg gagacgctcg ccacggttcc
     9421 cgacgaggcg aggcccgccc tcgtctacgg gttccgctac aaccgtgtcg acggccccgc
     9481 gccgacccta ggaggcgcgg tccggagcga gcgcttcccg aatcctctcg tctacgcgaa
     9541 gttcacctac gccttctccc gcgagcgctt cctctacgac gtcggcggcg aagctccgct
     9601 cggggatcac gcgcgattcc gggtcggcgg cgccgcctac cggcggaccg agaccgagga
     9661 cgagtggatc gtgggcgaga cggagaacac gctctacgcg ctcttcgcgc gcacggacta
     9721 tcgcgactac tacgagacca gcggctacga gggacacgtc gcgtgggatc cggggaccga
     9781 cttcggcgcg cgcgtggagg ggcggatgga ggatcagagg tcgctcgaga acgaggccaa
     9841 cgcgtccctt ttcggcgcgc acgaccgctt ccgccccaat ccgccgatcg aggacgggca
     9901 ggacggggtg ctcgcgctcg ggatgcggat cggccccgcg gcgattcctc cggagggagg
     9961 cacgcacggc gacgtcgtct acgagcgcgc cggcgacgcg ctccacggcg acttcgacta
    10021 cggccgcgtg cgcgcggtcg tgcgcacgcg cgtgcggctg tcaccgaagc aggacgctcg
    10081 cgcgcgcctg gtcggcgggt ccacgctcga cggaacgctc ccgccgcaga agctctggta
    10141 tctgggcggg atcagcacgc tccgcggcga ggatttcaag gagcggcaag gggaccagtt
    10201 ccttctcgcg aacgccgagt actacctcct cgtccggaag aacgtctggg gcttcggctt
    10261 cctcgactgg ggctccgcct ggttcggccg cgacaacatc gaccgccagc gattcctgct
    10321 cgacggcggc ctcggcgtcc ggatcggcca gggaccgctc gcgctgaccg cggcgcgcaa
    10381 cctccaggaa gcgcacgccg cgatccacgt cctcgtccgc ttcagcgaga ccttctgaga
    10441 tgctcgcccc ggggcgcgtc tgggtcgcgg cgtgggtcct cgcgctcgcc ctcgtcgcca
    10501 tcacgcctcg agccatcgtc gcggacgacc tcggcctcga agtcgatccg gtcgaggacg
    10561 tcgcaggcgc gatctgcgtg agctaccggg tcgcggagcc cttcacgccg cggctcgagg
    10621 agaccttgca gcgcggcatg ccggcgaccg tctcgttcga ggtcggactc tggaagcgcc
    10681 gcgcgctctg gttcgaccgg ctcgtgctcg cgatccggag cgatcacaag gtcgtctacg
    10741 acgactggcg ccaggccttc cgcatccgga gcggctcgaa tccaccgcgc gaccggagcg
    10801 ccccgtcgtt cgaagcgttg cgcgaggtgc tcttcacgca gtcgcgtctt ccactcgcgg
    10861 cgacatcgtc gctcgagccc ggcgcgacct actacgcgtc ggtgcgggtc acgatcaagc
    10921 cggtcgcccc cgaggatctg ggcgagatcg aggactggct ctccggagac aaggactccg
    10981 agcagtcgac cggcctgccg gattacctgc tcggcctcgc ggtcagtctg tcgggattgg
    11041 gcgagcggac ggagatggag aagggaccga agttcgtgcc ggcgcggctc tcggccgcgc
    11101 cggccacgac ggagtgacgg cggttcggcg gggacgcgcc tagggcgcgt ccgacgtgcc
    11161 cccttcgacg atcggcggcg acgcgggtgg aggaggaggc gtcgcgacgg acgccgcacc
    11221 cgtgatcggc gcttcgctca ccacgcccgg cgcgcccgcc ggagcggccg cgggcgaggg
    11281 acggcgacgc ggccaccaca tcccggtcgg caacccttcg ccgtgcggac cacgcgcgaa
    11341 gcgcgtctgc atcatggctc cgaatcccgc gatcgtgagg atcgccgcga gcgcgcccgt
    11401 cgcgatcgcg agcgcgatcg cgatcggatg gaagacgatc agcccgagga ccccgatcag
    11461 cttcccggcc accttgagcg tcgtgatcag gagcacgccc acggcgatcg cgagaagcgg
    11521 attcttcccc cgttccgtcg tccggccgag cacgcgctgg ccgaggaaca tggccccgtt
    11581 caggaagccc aggaacaccg cgaccaccac cccgagcacg agcccgatga tgccgagaat
    11641 caatccgagc agaagcacga tcgccacggg aatcccgatg atcgtgatcg cgaggatggc
    11701 gatcgcgatc gcgccgacca ggatcaagag cacgatcgcg gtcggaagcg ccacgagcga
    11761 gcccgcgaag ccggcgagcc cccagaggaa cgaccggccg aagcgcgcgt acatcgtgtc
    11821 gaccgcgtag accatccgct cccgcgcgag gagcagggcg agccacgcca ggaagaccgt
    11881 gagcgccagg cgcaccagcg tgtgcacgag ccagattccg gttcccacgg ccccgaagag
    11941 gccgaccatg ggccagaacg catggctatg ggtccagggc gagccgagcg agacgttgct
    12001 ccctccgacc gacgccgagt cgctcgtcgc gacgccgccg cccatcgaga ccgcgtcccc
    12061 ttccacaacg cccttgtcct tcacgttcac cgtgccgccg acggagacgc agtccccctt
    12121 cacgcggccg tagaccgtca cgtcgccgcc aatcgcgacc acgtcgccgt cgatcacctt
    12181 gtccgccggg atcacgatgt cctcgccgaa gcggaccagg ttgttgtcgt tcgagtcgag
    12241 atcgatattc acgtccggaa tgtccggcgc gtcggggacg tccggcggct tcggcaggtc
    12301 cgccttgatg cgggcggcct cgcgcttcgc gcgctccacc tcgcgccgga tgcgctcctc
    12361 ctggcgacgg atcttgagcg agctcgcgtc accctcctcg gacacgcgca cgcggaatcc
    12421 tccggtcgtg tcgggaggcg tggccggctt cggcggggtc ggcgtcgcgc cgcccgcgcg
    12481 tccggccgcg agaacgaggc cggtgaggag caggagccag gggagaagac ttagagaatg
    12541 cggagcgacc cgcgtccgcg cactagagcg ataggcacgc atggccgaca cctccttcac
    12601 gggtgcgaga cggacgaagc acgaagaaga gcgcgattcc cacggtgatc gcgacggcga
    12661 gcgcggcctg agcctcgctc gtctggagcg ccacccaggc tactcggggc agcgtcccga
    12721 gcgcgtggat cgcgctccag atcggccgtc cgatcggctc ccagaatgcg acctcgagcg
    12781 agagatgccg cacgaacttg accaccgcct cgagcgtgcc gcgcgccacg tgcaccggcc
    12841 aggtcgccgg ctcgggcaga ccccactggc gtgcgagctg cgggaggaac gcgacgagcc
    12901 cgatcgtgag cgcgaacgag agcgcgccgg cggtcgcgaa tccgccgcga agcgcgcgca
    12961 ggaggcgagc cggccgcgtc atccgcgcga cgatccgcgc ctcgagccac gcgggcgcct
    13021 cgagctcggg aagctccgcc aatccagcgg cgatcgcgcg atacgcgtcg agcttcaccc
    13081 ggcacgaggg gcacgcggtc acgtgcgcct cgaggacgcg cgcgacggcg ggatggagcg
    13141 cgccgtccac gtacgcggag agctcgggct cgcacgcctt gcaggtgagc tgggtcttca
    13201 tccttctcct cgcgccgtca atttccgccg cagcatctcg tgggcccggt ggatccggac
    13261 cttcaccgtt ccgagcggca tgttcatcga ttcggcgatg tcctcgtacg acagctcctg
    13321 cttgtagcgc aggaagagaa tcgcctggta gtgctccggc agctcccgga ccaggctctc
    13381 gaaccgcgcg tccgaccgct cgatccggta gccctcgtcg ggcccgggtc cggtatcggc
    13441 cggctcgatg gtcggtccat cctcgtcgcc cagatcctcg agcgcgatcg ggcggcgctt
    13501 gcgccggcgg atcgtgtcga gggccagatt cgacgtgatc ttgaagagcc acgtccggaa
    13561 cggccgggac gggtcgtagg tcccgagcga tcgcagcacg cggatgaagg tctcctgggc
    13621 cacctccgcg gcgtcgtccg cgctgccggt gatcttccag gccaggttca tgaccgctcc
    13681 ctgataacgc cggaccagct cccggtacgc gcgttcgtcg cgcgccaggc accgctccac
    13741 caggtctcgc tcatcgcttc ccacgtctac gggcggctct ccacgaaacc ctcgtgcact
    13801 ctacgtagct gccaccggcg aggtttcgcc ggatcggcta ccccgggctc cgtggtccgc
    13861 gggagggcgg accggaggcg ctgggcgggc acgctagggg ggccgttccc tgggcgtcaa
    13921 ggcttttccc ttgacggcca actcggccaa gccctagcgt gccttgctac tatgaccgaa
    13981 atcgagtcgg tattcgcccg ggagatcctg gattcgcggg gcttccccac gctggaggcc
    14041 gaggtggccc tggtcggggg cgccatcggg cgcgccgccg ttccctccgg ggcgtccacg
    14101 ggctcgcggg aggcgctcga gctccgggac ggggatgcca agcgctacct gggcaaaggg
    14161 gtcgcccagg ccgtccggaa cgtgaacgag gcgatcgccc ccgcgctggt gggcgagaac
    14221 gccgccgatc aggccgcgat cgacaagctc ctcatcgagc tcgacggcac gccgagcaag
    14281 aagaagctcg gcgcgaacgc gctcctcggc gtctcgatcg cctgcgcgaa ggcggccgcc
    14341 gacgcgcacg cgctcccgct ctatcgctac ctgggcggcg cgaacgcgcg cacgctcccc
    14401 gtgccgctca tgaacgtcat caacggcggc gcgcatgcgg acaacaatct cgacatccag
    14461 gaattcatga tcgtgcccgt cggcttcgac tccttcgcgg acgcgctccg cgccggatcc
    14521 gaggtcttcc acacgctcaa gaagctcctc cacgagaagg gcctcgtcac cgcggtcggg
    14581 gacgaggggg gcttcgcgcc gaatctgggc gcaaactccg aagcgctcga cttcctcctt
    14641 cgcgcgatcg agaaggcggg ctaccgcccc ggggagcagg tggcgctcgc gctcgacgtc
    14701 gccgcgtcc
//
LOCUS       DASTKC010000258        13454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1171287, whole genome shotgun sequence.
ACCESSION   DASTKC010000258 DASTKC010000000
VERSION     DASTKC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171287"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1472)
                     /locus_tag="VFR25_10780"
     CDS             complement(66..1472)
                     /locus_tag="VFR25_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9941571.1"
                     /translation="MSTRLYRFGLTVAALLLLSGTALAQPAAGPAFWVVPGGGAYWPP
                     AEWGVKDPVPSFGGILGLRISDPVAFELRGHYSKADSSKPYAAGPDLKLLHGEGNITW
                     FLSPGVPVSPYLTFGAGVVNGDTGGDSQSKFAWNAGAGVRIKVSDQMAVRLDGRDISY
                     KLAASTNPDDEGSRHSVEVFGGFSFGFGGGPSDEDQDGVSNKVDKCPGTPPGARVDAT
                     GCPLDGDGDGVFDGIDKCDGTPAGAIVDATGCPKDSDGDGVFDGIDKCENTPAGARVD
                     ATGCESDSDGDSVPDGIDQCEATPRGCTVNPNGCPTDADQDGVCDGLDQCPDTQSNVR
                     VDAKGCPMQVSVHETEMMETGMIRLQDVNFDTGKATIKPESFKVLDDVGQILGHWPQL
                     RIEIGGHTDSRGSDARNQQLSEARAKAVRDYLTNKFPELDAEQFTAVGYGERQPIATN
                     TTALGMAKTRRVEFKVLN"
     gene            1629..3158
                     /locus_tag="VFR25_10785"
     CDS             1629..3158
                     /locus_tag="VFR25_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET9941572.1"
                     /translation="MASDFAAAMESGYAFEGETLLLGAPLIEGKVAATARVRVPLAMM
                     NRHGLIAGATGTGKTKTLQGLAEELSAAGVPVFLADLKGDLSGLAAPGEPDPRVLERV
                     KAIGTPFTPAASPVEFLSLTGTRGIPVRATVSSFGPLLLARTLGLNDTQESALAMVFK
                     YCDDSKLLLLDLPDLRSVLQHLIGEGSAALEQYGALSKATVGVLLRKITELEQQGAGA
                     FFGEPEFEIEDLLRLTKDGRGVVSVLELDDLQDHPALFSTFMMWMLASLYRELPEIGD
                     APKPKLVFFFDEAHLLFEDAGKALRDQIEQVVRLIRSKGVGVFFVTHTPKDVPEEILA
                     QLGHRVQHALRAFSPDDEKALRATANTFPRSEFYDIPKVLTSLGIGEALVTVLSPRGT
                     PTPVAATLLRPPSSRMGPIAPDELKKIVEASPLRSEYAEAVDRESAREVLAARAGAAA
                     AAPAAAAKGKETAKETSLADTVGKALSSPAGKTLQRELVRGLLGVLGVRAATRRRRSS
                     W"
     gene            3203..3673
                     /locus_tag="VFR25_10790"
     CDS             3203..3673
                     /locus_tag="VFR25_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9941573.1"
                     /translation="MTSIASDIRIRPLSELDISRIVSIDERLTGVYRPEVWERRVGYY
                     LRRDPESCPVAEFEGKVVGFMFSDVRGGEFGLEETTGWIERFGVDPDYQGRSIGRRLF
                     DAVKSHMVQNEVVTIRTLVDKNDSDLAAFLRAVGFDNASLQALELRVAGAGATA"
     gene            3685..4998
                     /locus_tag="VFR25_10795"
     CDS             3685..4998
                     /locus_tag="VFR25_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET9941574.1"
                     /translation="MNVEQTTLPKKFHDAVVHWQRHNFPDYGLLPENWQTYFPNDPEF
                     CLCAKMEIGMGDTIEVGDKKGERRMIKPSEMGEEPARHLLAIIRAQASTEFGSIQQHE
                     GTLARAQDDEDRFWVLRVMAEELRHGYQMFHLLVSQDWSGVSGQKAEEMVEEILSMQT
                     GSHVLDAFNLEYDSFVDNVVFAAVIDRVGKYQLSMQKVCAYKPFAQSMPPMLREEAFH
                     LAAGVIPLRRWVQRAAEGNELISTQAIQRSFNKWLPRGLEMFGHEKGGDSNIRFGFKN
                     MKNAEAQSLYYEEVRKMIADLNQRYLRARLPKLSPEEAERLADRLMKDGGRHEGIDHE
                     DLIRIPHPSFFRRKGEPAFTMVGTHGESYTDVDSYLRHLKQNLPEPYLHSRDLKSYLE
                     TLRDVVAGRTTVDQAIRKMPKLKRVGGMCPCSRSVRWVVEDGTAA"
     gene            5018..5560
                     /locus_tag="VFR25_10800"
     CDS             5018..5560
                     /locus_tag="VFR25_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941575.1"
                     /translation="MIRTACLAIALFALTSPLQATAATAPAAAAPAPKRTCDAPEAHQ
                     FDFWIGDWDVKTPDGKQAGTNRVDAVLGGCAIQEHWAGVRGMTGTSLNMYIPSTGQWH
                     QTWIDDRGSLLLLDGAYKDGAMVLMGDGKPKAGSSTPSRERITWTRLDHGDVRQLWEQ
                     SLDGGTTWTVVFDGRYHKRA"
     gene            5950..8478
                     /locus_tag="VFR25_10805"
     CDS             5950..8478
                     /locus_tag="VFR25_10805"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET9941576.1"
                     /translation="MDGHLDDEAWKTATPVDQFTQRQPDEGKPCSERTEVRALIADDA
                     LFIGARLYDKDAKKIRSRLVRRDDDLESDYLAILIDAYHDRSTAVLFRVSPSGSINDA
                     TIAANGNQDFSWDPVWHAHTSIDSAGWTAEVEIPLSQLHYNTAADAVWGIQIRRWINR
                     RQELSEFSFTPLKDNASVARYGLLTGLGSVQAGKHLEVLPYATVRSEHFQSDPNDPFR
                     DGSDQFPAAGVDLKYGLTSSLTLNATVNPDFGEVEVDPAVVNLSAFETFYPEKRPFFI
                     EGANVFRFGDMQSYNNFNTTIAFHGRRIGREPQRSLDSQVYRFLDAPVHTTIAGAAKL
                     TGKTRNGWTIGVLDAVTPEERADYVDSASAEQKTPVEPLTNYFVSRVRRDYRQGNTVV
                     GAILTGVRRDLGDPDLNALLRKDAWAGGMDLNHYWSSRRWSLDAMFMGSHVKGSPDAM
                     ASTQTSSARYYQRPDADYLEYDPTRTSLNGYAGQLSINKIAGKHGQGSLTYQDWSPGF
                     EINDLGFQNAADSRGLSTLALYKDNTPHKVMRSWDAFAFSNWSWNYGGDRTYQEYAGE
                     AEGTLSNYWSGYVRGEWYPGSIDDRLTRGGPLSRVPPGGAFKASINTDSRRSTTLGLN
                     GALIWDDAGGHLRTLNASLSTRPTTALRVLFEPGVRKLLDHAQYVATEDDPTAVETYG
                     ARYVFASLHQTQISLDTRVEWTFSPKLTFQLYVQPLIVSGDYTRFKELRAPRQFEFDV
                     YGKQQGTVGSDSTGAVVIDPDAGGPAPSFSFDNPDFNFRSFRGNAVLRWEYRPGSALF
                     LVWQQGREATAPIGDFEFSRDWRALFDIDPENVIAVKATYWLAL"
     gene            8590..9114
                     /locus_tag="VFR25_10810"
     CDS             8590..9114
                     /locus_tag="VFR25_10810"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HET9941577.1"
                     /translation="MMLERFRSCCVLRAAAWIGALAVLGLAGCVPQTHEDAADVRGAV
                     DASNRQFMDAFARGDAARISTLYADDAQLFPPGNLPVQGRADIEALWRGVLALPVKEM
                     HLTTVEFTVHGEDASEIGRYTLVGNDGRELDAGKYIVLWKKGAGGWKLHRDMWNSNAP
                     VVTSPPSAPPDSTP"
     gene            9158..11125
                     /locus_tag="VFR25_10815"
     CDS             9158..11125
                     /locus_tag="VFR25_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941578.1"
                     /translation="MESPPTSLPASVAERARAALVTRYGATHADAMGRGVAQVAERWW
                     PEDGNEDEFVAFCSESWIESDAERDAAFARLEQAMEQVDGHLHEVRRELQRPIHLDLG
                     PVRPVDLSLQNLDLGSHVEEDLFRTKTAFFALLNFPVDTLAERLDQGRGWDRRRWARS
                     RFMDRFAERIPSSLIQKMGQVMREADLYIAEYNIHMDRLRTKDGRRPFPEGLRLISHW
                     GLRDELKGRYVEGKDGQEKQRMIQTVMERIVRQEIPASVRDNPDVDWCPETNEVVPNG
                     DRSSREPDTRYAYLLEVFHALKLADPFTPTAPTFVRRRFDLDRQIPEAEVRALLEAVL
                     GSNEVRELARRVESRLGRPLEPFDVWYSGFSPREATSEEELDRVVRDRYPSLEAFQRD
                     LPRVLQALGFAADRAAWLAERIVVDPARGPGHAMGAARRGDQSHLRTRAHNGMSYQTF
                     NVAMHELGHCVEQVFSLDAIDHWSLSGVPNTAFTEAFAFTFQERDLEILGYGEKGDES
                     RAREVLQSLWSTYEICGVSLIDMDVWNWLYAHPDATPGELREAVVAIARNVWNRWFAP
                     VLGRRDSDLLAIYSHIIDSALYTPDYAIGKIVAFQVAPALRGAAFAEGVERMTRQGRL
                     TPDAWMRGATGKGLSSEPLLAAAREALARTS"
     gene            complement(11136..11870)
                     /locus_tag="VFR25_10820"
     CDS             complement(11136..11870)
                     /locus_tag="VFR25_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET9941579.1"
                     /translation="MAEAEFHVFRGDAKGGTLQPYKIQVEEGMVVLDAIHHIQAHDAP
                     DLAVRWNCKAAKCGSCSAEVNGKPSLMCKTRLDSLPLEQPVVVKPIRSFPVIKDLVTD
                     VSWNYEVNKKIPPFTPKKDAPWRWMQEDIDRVQEFRKCIECFLCQNVCHVIREHGVKT
                     SFGGPRFFVRVAGLEMHPMDDADRTELLKDELGIGYCNITKCCTEVCPEDIHITDNAI
                     IPLKERVVDEHYDPLRKVFKLLTGKK"
     gene            complement(11880..>13454)
                     /locus_tag="VFR25_10825"
     CDS             complement(11880..>13454)
                     /locus_tag="VFR25_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9941580.1"
                     /translation="VAAALGHVDAKDSWKVHFRDTMLGGKQLNHWRMAQLHAQEAPDR
                     VRELEHWGAVFDRTQDGRILQRNFGGHSAPRLAHVGDRTGLEMIRTLQDHGVHQGFEV
                     FMEVTVSRLLVEGGRVVGAFAYRRENGRFIVFRAKSVVLACGGVGKSFRITSNSWEYT
                     GDGHALAYEAGAELIDMEFVQFHPTGMVWPPGVMGILVTEAVRGEGGILRNSKGERFM
                     ERYDPKRMELSTRDMVARAIYTEVKEGRGSPHGGAFLDISHKPADWVKKKLPSMYHQF
                     MELADVDITKGPMEVGPTTHYIMGGVRVEPDTGASTVPGLYAAGETSGGMHGANRLGG
                     NSLSDLLVFGRRAGLGAAEHAKSRSAQAPIDPAQIEAAERAMLEPFERADGPSPYDIH
                     AKLQDMMQSLVGIFRVEEDLAAAIREIARLKQETARVRVLGSREFNPGWHLAIDLKAM
                     LTCSEAIARSAQLRKESRGGHARLDFPKTEPEFGTKNTVVKKDGETMAVGQSPLPEMP
                     AELRAIVDAELEAVKV"
BASE COUNT         2185 a         4639 c         4426 g         2204 t
ORIGIN      
        1 cgacccggcg gaacctactc cttgggaacg aactgctgct tcgtgttctc cttccggagc
       61 gcttcctagt tgagcacctt gaactcgacc ctgcgggtct tcgccatgcc cagcgccgtc
      121 gtgttggtgg cgatgggctg cctctctccg tagccgaccg ccgtgaactg ctcggcgtcc
      181 agctcgggga acttgttggt gagatagtcg cgcacggcct tggcgcgcgc ctcgctgagc
      241 tgctggtttc gcgcgtccga gccgcggctg tccgtgtgac cgccgatctc gatccggagc
      301 tgcggccagt gcccgaggat ctgtcccacg tcgtcgagga ccttgaacga ctcgggcttg
      361 atcgtcgcct tgccggtgtc gaagttgacg tcctggagcc ggatcatccc cgtctccatc
      421 atctcggtct cgtgcacgct gacctgcatc gggcagccct tcgcgtccac gcggacattc
      481 gactgcgtgt cggggcactg atcgagcccg tcacacacgc cgtcctgatc cgcgtcggtc
      541 gggcagccgt tcggattgac cgtgcagccg cgcggggtgg cctcgcactg atcgatcccg
      601 tcgggcacgc tgtccccatc ggaatcgctc tcgcatccgg tcgcgtccac gcgcgctccg
      661 gcgggcgtgt tctcgcactt gtcgatgccg tcgaacacgc cgtcgccgtc cgagtccttc
      721 gggcatccgg tcgcgtcgac gatcgcgccc gccggcgtgc cgtcgcactt gtcgatgccg
      781 tcgaacacac cgtccccgtc gccgtcgagc ggacatccgg tggcgtccac gcgcgcgccc
      841 ggcggcgtgc ccgggcactt gtccactttg ttgctcacgc cgtcctggtc ctcgtcggac
      901 ggccctccgc cgaatccgaa cgagaagccg ccgaagacct cgaccgagtg gcgactgccc
      961 tcatcgtcgg gattcgtcga ggcagcgagc ttgtagctga tgtcccgccc gtcgagccgg
     1021 accgccatct gatcggagac cttgatgcgg actccggcgc cggcgttcca cgcgaacttg
     1081 gactggctgt cgccccccgt gtccccgttc acgacaccgg cgccgaacgt gagatagggg
     1141 ctcaccggaa cgccggggct gaggaaccag gtgatgttcc cttcgccgtg gaggagcttg
     1201 agatcgggtc ccgcggcgta gggcttggag ctgtcggcct tcgagtagtg gccgcggagc
     1261 tcgaaggcga ccgggtcgct gatccgaagc ccgagaattc cgccgaacga cggaactgga
     1321 tccttcacac cccattccgc ggggggccag taggcgccgc cgccggggac gacccagaag
     1381 gcgggtccag cggcaggctg agcgagagcc gtgccagata ggagaaggag tgccgccaca
     1441 gtaagtccaa atcggtacag cctcgtgctc acgttgccct ccaggagctt cgaaacgcgt
     1501 ggccgtgctc gacggagaaa tggagtgggg ctcgacggcg cgcaatgtat cggaaacaag
     1561 ggcgcgcctt caacgggaat caacgccgcg ttgacggggc ggcccgctgc tcctagattc
     1621 gaagcacgat ggcgagcgat ttcgcggctg cgatggagtc tggatacgcg ttcgaggggg
     1681 agacgctcct tctcggtgct ccgctcatcg agggcaaggt cgcggcgacc gcgcgtgtcc
     1741 gagtgccgct cgcgatgatg aaccgccacg gtctcatcgc gggcgcgacc ggcaccggaa
     1801 agacaaagac gctccagggg ctcgccgaag agctgagcgc cgccggcgtt cctgtcttcc
     1861 tcgctgatct gaagggcgac ctgagcggac tcgcggctcc gggtgagccc gatccgcgcg
     1921 tgctcgagcg cgtcaaggcg atcgggacgc cgttcacgcc cgcggcgtcg ccggtcgagt
     1981 tcctgagctt gaccggaacg cgaggcatcc cggtgcgcgc caccgtgtcc tcgttcggac
     2041 cgctcctcct cgcgcgcacc ctggggctga acgacaccca ggagagcgcg ctcgcgatgg
     2101 tcttcaagta ctgcgatgac tcgaagcttc tcctcctcga cctacccgac ctccgctcgg
     2161 tgctccagca cctgatcggg gaaggatccg ccgcgctaga gcagtacggc gcgctctcga
     2221 aggcgacggt cggggttctt ctgcgtaaga tcaccgagct ggaacagcag ggcgccggag
     2281 ccttcttcgg agagccggaa ttcgagatcg aggacctgct ccgcctgacc aaggacgggc
     2341 gcggcgtcgt gagcgtgctg gagctggacg atcttcaaga tcatccagcg ctcttctcca
     2401 cgttcatgat gtggatgctc gcgtccttgt atcgcgagct tccggagatc ggcgacgcgc
     2461 cgaagccgaa gctcgtcttc ttcttcgacg aggcgcatct cctcttcgag gacgccggga
     2521 aagcgctccg cgatcagatc gagcaggtcg tgcgcttgat ccgatcgaag ggcgtcggcg
     2581 tcttcttcgt cacgcacacc ccgaaggacg tccccgagga gatcctggcg cagctcgggc
     2641 atcgcgtgca gcacgcgctg cgcgcgttct cgcccgacga cgagaaggcg cttcgcgcga
     2701 ccgcgaacac gttcccgcga tcggagttct acgacatccc caaggtcctg acctcgctcg
     2761 ggatcggaga agcgctcgtc acggtcctct ccccgcgcgg caccccgacg ccggtcgcgg
     2821 ccacgctgct tcgtccgccc tcgagccgga tgggtcccat cgccccggac gagctcaaga
     2881 agatcgtcga ggcgtcgccg ctccgctcgg agtacgccga ggcggtggac cgcgagagtg
     2941 cccgcgaagt cctggccgca agggccggag cggctgccgc cgcgccggcc gcggcggcga
     3001 agggcaagga aaccgccaag gaaacctccc ttgcggacac cgtggggaag gcgctctcct
     3061 cgcccgcggg caagacactt caaagggagc tcgtccgggg cctcctgggg gtactcggcg
     3121 tgcgcgcggc cacgcgccgg cgccgctcca gctggtagcg gaaccgctcg cgcgggtccc
     3181 gcccggatgg tattctgagc gcgtgacttc catcgcttcg gacattcgga tccgccccct
     3241 ttccgagctc gacatctcgc gcatcgtctc gatcgacgag cggctcacgg gggtctatcg
     3301 accggaggtc tgggagcgcc gcgtgggcta ctacctgcgc cgggatcccg agagctgtcc
     3361 cgtggccgag ttcgagggca aggtggtcgg cttcatgttc tcggacgtcc gtggcggtga
     3421 gttcggtctc gaggagacca ccggctggat cgagcggttc ggcgtcgatc cggactacca
     3481 ggggcgctcc atcggccggc ggctcttcga cgccgtgaaa tcccacatgg tccaaaacga
     3541 ggtcgtcacc atccgcactc tcgtcgacaa gaacgattcc gatctcgccg cgttcctgcg
     3601 cgcggtcggg ttcgacaatg catcgctcca ggcgctcgag cttcgcgtgg cgggcgcggg
     3661 cgcgacggct tagcgaaagg ctccatgaac gtcgagcaga ccacgctgcc gaagaagttc
     3721 cacgacgccg tcgtccactg gcagcggcac aacttccccg attacggact cctgcccgag
     3781 aactggcaga cctatttccc caacgatccc gagttctgcc tctgcgccaa gatggagatc
     3841 gggatggggg acacgatcga ggtcggggac aagaagggcg agcgccggat gataaagccc
     3901 tcggagatgg gggaggagcc ggcgcggcac cttctcgcca tcatccgcgc gcaggcgtcg
     3961 acggaattcg gctccatcca acagcacgag ggaacgctgg cccgcgcgca ggacgacgag
     4021 gatcgcttct gggtgctccg cgtgatggcc gaggagctcc ggcacggcta ccagatgttc
     4081 catctcctcg tgtcgcagga ctggagcggg gtctcggggc agaaggcgga agagatggtc
     4141 gaggagatcc tctcgatgca gacggggtcg cacgtcctcg acgccttcaa cctcgagtac
     4201 gactcgttcg tggacaacgt ggtcttcgct gcggtcatcg atcgggtcgg caagtaccag
     4261 ctctcgatgc agaaggtctg cgcgtacaag ccgttcgcgc aatcgatgcc gcccatgctc
     4321 cgggaggaag cgttccatct cgccgccggc gtgattccgc ttcgccgctg ggtccagcgc
     4381 gccgcggaag ggaacgagct catctccact caggcgatcc agcgctcctt caacaagtgg
     4441 ctcccccgcg ggctcgagat gttcggccac gagaaggggg gcgactccaa catccggttc
     4501 ggcttcaaga acatgaagaa cgcggaggcg caatcgctct actacgagga ggtccgcaag
     4561 atgatcgcgg acctgaacca gcgctacctc cgcgcgaggc tccccaagct ctcgcccgag
     4621 gaggcggagc ggctggccga tcgtttgatg aaggacggcg ggcgccacga ggggatcgat
     4681 cacgaggatc tgatccggat tccgcacccg tccttcttcc gcagaaaggg cgagcccgcg
     4741 ttcaccatgg tgggcacgca tggcgagtcc tacacggacg tcgactcgta tctacgccac
     4801 ctgaagcaga atctccccga gccctacctc cacagccgcg atctgaagag ctatctggaa
     4861 acgcttcgag acgttgtagc cggccggacc accgtggatc aggcgatcag gaagatgccg
     4921 aagctgaaac gcgtcggcgg gatgtgcccc tgctcgcgct cggtccgctg ggtggtggag
     4981 gacggtacgg ccgcctgagc cgctccgata cccgcacatg atccgaaccg cttgccttgc
     5041 gatcgctcta ttcgctctga cctcgccgct tcaagccaca gcagccaccg cgcccgcggc
     5101 cgccgcgccc gctcccaagc gcacgtgcga cgctcccgaa gcgcaccaat tcgacttctg
     5161 gatcggcgac tgggacgtca agacgcctga cggaaagcag gcgggaacga accgggtgga
     5221 cgcggtgctt ggagggtgcg cgatccagga acactgggcc ggcgtgcgcg gcatgaccgg
     5281 gaccagcctc aacatgtaca tcccgtccac cggacagtgg catcagacct ggatcgacga
     5341 tcgcggcagc ctcctgctcc tggatggcgc ctacaaggat ggagcgatgg tgttgatggg
     5401 cgacgggaag cccaaggccg gatcctccac gccgtcgcgc gaacgaatca cctggacccg
     5461 cctcgatcac ggcgacgtcc gccagctctg ggagcagtcg ctggacggcg gtacgacctg
     5521 gacggtcgtg ttcgacgggc ggtaccacaa aagagcctga ccccgcgcgg caggatcccc
     5581 ggagggaccc tcctgccttc caaccaaatc ccggccagga ccgtctcatt cctcgtcggc
     5641 gacgccgagg aaacccggcg cgccagccag ccgtagatgg aggaggatcg ggaaggagcg
     5701 tttcgaggga tcgtcatgcg cgcacggaat cgctggctgg ccgcggctct gccgcttctt
     5761 acgacatgga tcctgctcct ctcaaccact cccgcatcag ctgcccaatc actgcccggt
     5821 agcggcacgc ccgccgattc gaccgccccg gggcccaacg tcttcccccc gcccgccgga
     5881 ccgggagcct cggcctctcc tgccgacctc ccccagcttc atgcagcacc gctcacgggc
     5941 acgattcgcg tggacgggca tctcgacgac gaggcatgga agaccgcgac gccggtcgat
     6001 caattcacgc agcggcagcc ggatgaaggg aagccgtgct ccgagcgcac cgaggtgcgc
     6061 gccctgatcg cagacgacgc gctcttcatc ggcgcgcgcc tctacgacaa ggacgcgaag
     6121 aagatccgct cccggctggt gcgccgcgac gacgacctcg agtccgatta cctcgcgatc
     6181 ctgatcgacg cgtatcacga ccgctcgacc gcggtgctct tccgcgtgag cccgtccggt
     6241 tcgatcaacg acgccaccat cgcggcgaac ggcaaccagg acttctcctg ggatccggtc
     6301 tggcacgctc acacttccat cgacagcgcg ggctggaccg ccgaagtgga gattcccttg
     6361 tcgcagctcc actacaacac ggcggcggac gcggtctggg gcatccagat ccgccggtgg
     6421 atcaaccggc ggcaggagct gtcggagttc tcgttcacgc ccctcaagga caacgcgagc
     6481 gtcgcgcggt acgggctcct caccgggctg ggatcggtcc aggctggaaa gcacctcgag
     6541 gtccttccgt acgcgaccgt ccgatcggag cacttccaga gcgatccgaa cgatccgttc
     6601 cgcgacggct cggatcagtt cccggccgcg ggcgtggatc tcaagtacgg cctgacctcg
     6661 agcctcacct tgaacgcgac ggtgaatccg gacttcggcg aggtggaggt ggacccggcg
     6721 gtggtgaatc tctcggcgtt cgagacgttc tatcccgaga agcggccctt cttcattgag
     6781 ggggcgaacg tgttccgctt cggcgacatg cagagctaca acaacttcaa cacgacgatc
     6841 gccttccacg gccgccgcat cggccgcgag ccgcagcgct cgctcgactc gcaggtgtat
     6901 cgcttcctcg acgcgccggt gcacacgacg atcgcagggg cggccaagct cacggggaag
     6961 acgcggaacg gctggacgat cggcgtgctg gacgccgtga cgcccgagga gcgcgccgat
     7021 tatgtggact ccgcgagcgc ggagcagaag acgcccgtcg agccgctcac caactacttc
     7081 gtcagccgcg tgcggcgcga ctaccggcag gggaacacgg tcgtcggcgc catcttgacc
     7141 ggagtccgcc gcgatctcgg cgatcccgac ctcaacgcac tcctccgcaa ggacgcttgg
     7201 gccggcggga tggatctcaa tcactactgg tcgtcccgcc ggtggtccct ggacgcgatg
     7261 ttcatgggaa gccatgtgaa ggggagcccg gacgcgatgg catcgaccca gacctcgagc
     7321 gcgcggtact accagcggcc ggacgccgac tatctcgagt acgaccccac gcgcacgagc
     7381 ttgaacggct acgccggaca gctctccatc aacaagatcg cgggcaagca cggccagggc
     7441 tcgctcacct accaggactg gagccccggg ttcgagatca acgacctggg cttccagaac
     7501 gcggcggaca gccgcgggct ctccacgctc gcgctctaca aggacaacac gccccacaag
     7561 gtgatgcgga gctgggacgc attcgccttc tcgaactggt cgtggaatta cggcggcgac
     7621 cgcacgtacc aggagtacgc cggggaagcc gaagggacgc tctccaacta ttggtccggc
     7681 tacgtgcgcg gcgagtggta cccggggagc atcgacgacc ggctgacgcg cgggggaccg
     7741 ctgtcgcgcg tgccgccggg aggcgcgttc aaggcctcga tcaacacgga ctcccgacgg
     7801 tcgaccaccc tcggcttgaa cggcgcgctc atctgggacg acgcgggcgg acacttgcgc
     7861 acgctcaacg cttcgctctc gacgcgtccg accaccgcgc tccgcgtcct cttcgagccc
     7921 ggcgtccgga agctcctcga tcacgcgcaa tacgtggcga cggaggacga tccgacggcg
     7981 gtcgagacct atggcgcgcg gtatgtcttc gcttcgctcc atcaaaccca gatctcgctc
     8041 gacacgcgcg tcgagtggac gttctcgccc aagctgacct tccagctcta cgtccagccc
     8101 ttgatcgtga gcggtgatta cacgcgcttc aaggagctcc gcgctccgcg ccagttcgag
     8161 ttcgacgtct acggcaagca gcagggaacg gtgggatcgg attccaccgg ggccgtcgtc
     8221 atcgatcccg acgcaggcgg cccggctccg tccttcagct tcgacaaccc cgacttcaac
     8281 ttccgatcgt tccgcgggaa cgcggtcctc cgatgggagt acaggcccgg ctccgccctc
     8341 ttcctcgtgt ggcagcaggg gcgcgaagcc accgcgccga tcggggattt cgagttctcg
     8401 cgcgactggc gcgccctctt cgacatcgac cccgagaacg tcatcgccgt caaggcgacc
     8461 tactggctcg ccctctaacc ccagtccagg aacccagctc cgtccatttg cccccggtct
     8521 gtggtagggt aaacgcctgc cccgccgcgt ccgcgcgaca cctgtaccgg gaaccgagga
     8581 ggaaacagga tgatgctgga acggtttcgt tcgtgctgcg tgctacgggc tgccgcctgg
     8641 atcggggcac tcgccgtcct cggcctcgcg ggctgcgtgc cccagacgca cgaggacgcg
     8701 gccgacgttc gcggagccgt cgatgcgagc aatcgccagt tcatggacgc attcgcgcgc
     8761 ggcgacgcgg cccgcatctc cacgctctac gccgatgacg cgcagctctt cccccccggc
     8821 aacctgccgg tccaggggcg cgcggacatc gaagcgctct ggcgcggcgt gctggccctc
     8881 ccggtcaagg agatgcacct gaccaccgtc gagttcaccg tgcatggcga ggacgcgtcc
     8941 gagatcgggc gctacacgct cgtcggcaac gacggacggg agctggatgc cggcaagtac
     9001 atcgtgctgt ggaagaaggg cgccggagga tggaagctgc atcgcgacat gtggaactcg
     9061 aacgcgccgg tcgtcacgtc gcccccgtcg gcgccgcccg attccacccc atgacgccga
     9121 gcaccaagag cgccccttga acccgaccac gcacgccatg gaatcacccc ccacatcgct
     9181 tcccgcgagc gtggccgagc gcgcccgcgc cgctctggtc acccggtacg gcgcgacgca
     9241 cgcggacgcc atggggcgcg gtgtcgcgca ggtggccgag cggtggtggc cggaggacgg
     9301 gaacgaagac gaattcgtcg ccttctgctc cgagagctgg atcgagtccg acgccgagcg
     9361 ggacgccgcg ttcgcccgcc tcgagcaggc gatggagcag gtcgacggac acctccacga
     9421 ggtgcgccgc gagctccagc gcccgatcca tctcgatctc ggaccggtcc gccccgtcga
     9481 tctctccctc cagaacctgg atctaggctc gcacgtggag gaggatctct tccgcaccaa
     9541 gaccgccttc ttcgcgctgc tcaacttccc ggtggacacg ctggccgagc gcctcgatca
     9601 gggacgcggc tgggatcgcc gacgctgggc acgctcccga ttcatggatc gcttcgcgga
     9661 gcgcatcccg tcctcgctca tccagaagat gggccaggtc atgcgcgagg cggacctcta
     9721 catcgcggag tacaacatcc acatggatcg cctccgcacg aaggacggcc gccgcccgtt
     9781 tcccgaagga ctccggctga tctcgcactg gggtctgcgc gacgagctca agggacgcta
     9841 cgtggagggg aaggacgggc aggagaagca gcgcatgatc cagaccgtga tggagcgcat
     9901 cgtgcgccag gagatcccgg cgtcggtgcg cgacaacccc gacgtggact ggtgtcccga
     9961 gaccaacgag gtggtgccga atggcgaccg ctcgtcgcgc gagccggaca cgcgctacgc
    10021 gtacctgctc gaggtcttcc acgcgctcaa gctcgccgat ccctttacac cgacggcgcc
    10081 cacgttcgtg cggcgccgct tcgacctgga ccggcagatc ccggaggcgg aggtgcgcgc
    10141 gcttctcgag gcggtcctcg gctcgaacga ggtccgcgag ctggcccgcc gcgtcgagtc
    10201 gcgcctcggc cgcccgctcg agccgttcga tgtctggtat tcgggattct cgccccgcga
    10261 ggccacgagc gaggaggagc tggaccgggt ggtgcgcgat cgctatccgt ccctcgaggc
    10321 attccagagg gacctcccgc gcgtgctcca ggcgctcggg ttcgccgccg atcgagccgc
    10381 gtggctcgcg gagcgcatcg tggtcgatcc ggcgcgcgga ccggggcacg ccatgggcgc
    10441 cgcgcggcgc ggggaccagt cgcacctgcg cacccgggcg cacaacggca tgagctacca
    10501 gaccttcaat gtcgcgatgc acgagctggg gcactgcgtg gaacaggtct tctcgctgga
    10561 cgccatcgac cactggtcct tgagcggcgt tcccaacacg gccttcaccg aggcgttcgc
    10621 cttcacgttc caggagcggg acctcgagat cctgggctat ggcgagaagg gggacgagtc
    10681 gagggcgcgc gaggtgctcc agtcgctctg gagcacgtac gagatctgcg gcgtgagctt
    10741 gatcgacatg gacgtttgga actggctcta cgcccatccc gacgcgacgc cgggggagct
    10801 gcgcgaggcc gtcgtcgcga tcgcgcgtaa cgtctggaac cgctggttcg ctccggtgct
    10861 cggccggcgc gacagcgatc tcctcgcgat ctactcccac atcatcgact ccgcgctcta
    10921 cacccccgac tacgccattg gaaagatcgt ggccttccag gtcgcgccgg cgctccgagg
    10981 cgccgcgttc gccgagggcg tcgagcgcat gacgcggcag gggcgcctca ctccggacgc
    11041 gtggatgcgc ggcgccacgg gcaaggggct ctcgtcggag ccgctcctcg ccgcggcgcg
    11101 cgaggcgctc gcccgcacgt cctgatccgg cggggttact tcttcccggt caggagcttg
    11161 aagaccttcc ggagcggatc gtagtgctcg tcgacgacgc gctccttgag cgggatgatc
    11221 gcgttgtcgg tgatgtggat gtcctcgggg cagacctccg tgcagcactt cgtgatgttg
    11281 caatagccga tcccaagctc gtccttcagc agctccgtcc ggtccgcgtc gtccatcggg
    11341 tgcatctcga gccccgctac gcgcacgaag aagcgcgggc cgccgaatga agtcttcacc
    11401 ccgtgctccc ggatcacgtg gcagacgttc tggcagagga agcactcgat gcacttccgg
    11461 aactcctgca cgcgatcgat gtcctcctgc atccaccgcc agggggcgtc cttcttcggc
    11521 gtgaagggcg ggatcttctt gttcacctcg tagttccagg acacgtcggt gacgaggtcc
    11581 ttgatcacgg ggaaggaccg gatcggcttc acgaccaccg gctgttcgag cgggagcgag
    11641 tcgagccgcg tcttgcacat gaggctcggc tttccgttca cctcggcgct gcaggagccg
    11701 cacttggccg ccttgcagtt ccagcggacc gccaggtcgg gggcgtcgtg cgcctggatg
    11761 tggtggatgg cgtcgaggac gaccatccct tcctcgacct ggatcttgta gggctggagc
    11821 gtcccgccct tggcatctcc gcggaagacg tggaattcgg cttcggccat cgcgcccccc
    11881 tagaccttca ccgcttcgag ctcggcgtcg acgatcgcgc ggagctccgc gggcatctcc
    11941 ggaaggggcg actgccccac cgccatggtc tcgccgtcct tcttcaccac ggtgttcttg
    12001 gtcccgaact cgggctccgt cttcgggaag tcgaggcgcg catgcccgcc ccggctctcc
    12061 ttccgcagct gcgcgctccg cgcgatcgcc tcactgcatg tgagcatcgc cttgagatcg
    12121 atcgcgagat gccatccggg attgaactcg cgcgagccca ggacccggac gcgcgcggtt
    12181 tcctgcttga gccgcgcgat ctcacggatg gccgcggcga gatcctcctc gacgcggaag
    12241 atgccgacga gggactgcat catgtcctgg agcttggcgt ggatgtcgta gggggatgga
    12301 ccgtcggcgc gctcgaacgg ctcgagcatc gcgcgctcgg ccgcctcgat ctgcgcggga
    12361 tcgatcgggg cctgggcgga gcgcgacttc gcgtgctccg ccgctccgag cccggcgcgg
    12421 cgcccgaaga cgaggaggtc ggagagggaa ttcccgccga gccggttggc tccgtgcatc
    12481 cctcccgagg tctcgcccgc ggcgtagagc cccggcaccg tcgatgcccc cgtgtccggt
    12541 tcgacccgca cgccacccat gatgtagtgc gtcgtcgggc ccacttccat cgggcccttc
    12601 gtgatgtcga cgtcggccag ctccatgaac tggtggtaca tggacggaag cttcttcttc
    12661 acccagtccg ccggcttgtg cgagatgtcg aggaacgcgc cgccgtgggg cgatccgcgc
    12721 ccttccttga cctcggtgta gatcgcgcgc gccaccatgt cgcgggtcga gagctccatc
    12781 cgcttcgggt cgtacctctc catgaagcgc tcgcccttcg aattgcggag gatgccgcct
    12841 tcgccgcgca ccgcctcggt gacgaggatg cccatgacgc cggggggcca gaccattccg
    12901 gtcggatgga actgcacgaa ctccatgtcg atcagctcgg cgcccgcttc gtacgcgagg
    12961 gcgtggccgt cgcccgtgta ctcccaggag ttggacgtga tccggaacga cttgcccacg
    13021 ccgccgcagg cgagcacgac cgacttggcg cggaacacga tgaagcgccc gttctcgcgc
    13081 cggtacgcga acgcaccgac gacgcgaccg ccctcgacga ggaggcggct gacggtgacc
    13141 tccatgaaga cttcgaagcc ctgatggacg ccgtggtctt ggagcgtgcg gatcatctcg
    13201 aggcccgtgc ggtcgccgac gtgcgccagg cgcggcgccg agtggccgcc gaagttccgc
    13261 tggaggatgc ggccgtcctg ggtgcggtcg aagacggcgc cccagtgctc cagctcgcgc
    13321 acgcggtcgg gagcttcctg cgcgtggagc tgcgccatcc gccagtgatt gagctgcttc
    13381 ccgccgagca tcgtgtcgcg gaagtggacc ttccaggagt ccttggcatc cacgtgcccg
    13441 agcgccgcgg ccac
//
LOCUS       DASTKC010000259        23172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_696535, whole genome shotgun sequence.
ACCESSION   DASTKC010000259 DASTKC010000000
VERSION     DASTKC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 23172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23172
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696535"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..684)
                     /locus_tag="VFR25_10830"
     CDS             complement(268..684)
                     /locus_tag="VFR25_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941581.1"
                     /translation="MTRDIKESVSLEQRLERVERENRRFKVLGSVVLLGMAGVLCMGL
                     TSNPSKKLEAEVLVLKNAQGKSQMILGAGDDGPALTILDKNGKLRVNLGVGQDGPALD
                     LLDAAENPRAQLMITEDQGPLLNFYDAKGSQVSLKP"
     gene            864..1760
                     /locus_tag="VFR25_10835"
     CDS             864..1760
                     /locus_tag="VFR25_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941582.1"
                     /translation="MRKGRYVQLVSYRVAVVVLAILTGALAMHERGTGASVWLLSAIL
                     GVAAASALVWPLVVPARDRTMSVYSIGPAFFLAGMFLLPPPALVGIITFAVLLSGLIR
                     GDRAYRTAFRLASMVLVFAGFAIWFRLSPQGSEVLYKPAARATLEMAIGGAALTALLI
                     LKSLEIRLEQGPEHTPHWGAFQKPAIMESMLCLAFSTSTVVLARIHTGFLTVVGFQMV
                     MVWWFLHRYAAYTTGLARVTDRRKRPRAILWDAATRDALRVGPFAAPLPSLRRFPKEA
                     TAAEGREDDDEDWKSGRAGGAR"
     gene            complement(1757..3010)
                     /gene="ispG"
                     /locus_tag="VFR25_10840"
                     /gene_synonym="gcpE"
     CDS             complement(1757..3010)
                     /gene="ispG"
                     /locus_tag="VFR25_10840"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET9941583.1"
                     /translation="MPTITIPLDSQGSTSVPRRRSVLVRIGSVTIGGNRPVAVQSMTN
                     TDTADAAGTVAQVIALAEAGSELVRVTVNTPEAARSVPEIVARTRDAGIAAPIVGDFH
                     FSGHLLLSQFPDCARALDKYRINPGNVGSGERHDEHFSQIIRIAVANGKPVRIGVNWG
                     SLDRELLTALMDQNARSAKPRDDREVTLDAMFESAVRSAALAEEFGQPHDAIVLSAKV
                     SGVRDLIAIYRRLAAETDYPLHLGLTEAGMGAKGVVASTAGLSTLLLEGIGDTIRVSL
                     TPQPGGDRREEVRICQQILQSLELRHFEPQVTSCPGCGRTTSIFFQELAERVNLRLRE
                     KMPVWRTAYPGVEGLRVAVMGCVVNGPGESRHADIGISLPGTAEDPKAPVFVDGALKT
                     TLQGPRIAEEFLALVEEYVARRFGS"
     gene            3062..3814
                     /gene="ubiE"
                     /locus_tag="VFR25_10845"
     CDS             3062..3814
                     /gene="ubiE"
                     /locus_tag="VFR25_10845"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET9941584.1"
                     /translation="MTTPPIAATTGAAGAEPQAPAAPRVRTMFASIAGSYDRANQILS
                     LGLHHRWRERTVRLSGARPGMRVLDCATGTGDLALAFRRAVGQDGDVVGTDFCAEMLA
                     LAPGKAARAGLPVRFEIADVLHLPYPDRSFDIASIAFGIRNVEDPARGLLELARVTRA
                     GGRVVVLEFGQPGGAWFGPLYRFYSHSVLPRLGGVISGQRSAYEYLDRTSSRFPAGER
                     FLAMMRDTGAYSDARAHALTGGVAYVYVGEVA"
     gene            3811..4251
                     /locus_tag="VFR25_10850"
     CDS             3811..4251
                     /locus_tag="VFR25_10850"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9941585.1"
                     /translation="MSFQATIPVRFQDVDAAGVLFFGRIFDYCHQAYEELIGSFGVDR
                     AHYFAGAEYLVPIAHARADYRSPIRLGERVHVTIEILRVGRASVRLRYRVTGPKADDL
                     RAEVETVHAFVDRATMRPISIPDPLRGFFLEHLVPDAAAEGVTK"
     gene            complement(4258..5547)
                     /locus_tag="VFR25_10855"
     CDS             complement(4258..5547)
                     /locus_tag="VFR25_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9941586.1"
                     /translation="MPSRPDIDAAEALRLAARREHELLAISELSRELTVGLDLYALAD
                     LVLLNLMGQLGASRAALWLLPDGSERRPVLLRSHGMSRRMADALGATVAPDALQALGE
                     ERRILGAGEFQARLHPATVALARREEIELLAPLTSRGETLGLVALGPGAGGAPYGPME
                     MDALQASLGMIGVALQNATLYNRLLENNRQLRMANEELTDLDRLKSEFLRNLNHELRT
                     PLTVIIAYLSSVEDQGGLPDTVREFTGVCLDESKKLKAMLENLLDFGAATRGSLPLDI
                     RRGDLTLVLRAYYEEWLPGITEGLRELVFEAPEEPLTTAFDRHRVLQIVEALVDNATK
                     FTPRGTRITLRLAVDGETAVVEVEDDGPGIPPERLTQIFGSFRQGDGSPTREVGGLGL
                     GLAFALELAHRMNGRLTASSQLSRGSIFRLSLPAVAR"
     gene            complement(5531..5872)
                     /locus_tag="VFR25_10860"
     CDS             complement(5531..5872)
                     /locus_tag="VFR25_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9941587.1"
                     /translation="MAEPLEVVSGVHGVIVRVVGRLDAKSVPALLAQCADVRASGRNL
                     IVNLSRVEFIASSGVGTLLSVAEEFRKAGRTVRFASPSTPVLTVIQLLNLEPFLPIDS
                     SEEEAERALPA"
     gene            complement(5856..7889)
                     /locus_tag="VFR25_10865"
     CDS             complement(5856..7889)
                     /locus_tag="VFR25_10865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9941588.1"
                     /translation="MTERKTKVVWSIRWIVAVAAVLLTAGAVASVGTVAERNTRRALT
                     AEIETRLLLDARTLALASTGAMLGDYPELTLHPLAKELLARQPELTVIVVADNQGVVQ
                     GHPDVRSLGQSYSLPSRLLPIEDARRLARGETLGEDGGTLLAAVPVTHPDGRRLGLAV
                     VGMPRSYLDRVITDARRQQIAVLALFLAAAVAVPLILVSQLLRPIAALRHGIERIGRG
                     ELDRPVVLRDRTELGLLAAAINQMALELKRAQADLVERERLAHEMELAQRIQTSLLPK
                     GTTRAGAFVIQGYHRPAAEVGGDYYDVFDRPDGTIGVVVADVAGKGLAGCLVMSMVFS
                     LLRAYRDGIASPSSLLAQVDRRLAETLQPGSFVTLFYGVLDPSAGRITYASAGHNPLL
                     RYRARDRSVALLATRGIPIGAVRGGAIATTLEDRKLDLEPGDVLVQYTDGVNESMNAR
                     GEQFGLERLGQTVAAAAGGGASSVIEAVVSAIGGWTDNAPPGDDETLLVIAHETQADA
                     NTAAGALSPGDSEELLRQARGQGRTWTLPARMEALEGIGAWLRSCPPAGALSERELAL
                     LTTALYEVCANIVEHGYREDPSRSLDLGWVSDGRGETMFLVRDDGAPFAPPAAADLDF
                     GDPGVRSKGRGLGLQIIRRATRQAAYFPGTREGNITVLRFPSPREEAQRGRTP"
     gene            complement(7886..9883)
                     /locus_tag="VFR25_10870"
     CDS             complement(7886..9883)
                     /locus_tag="VFR25_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HET9941589.1"
                     /translation="MIRRFVIAWIAALTVLAAPPARGEDDGGTQSIFATGTGVRAIAL
                     GGAYAALASDASAMAWNPAGLGLVPRREIQAGRFQYGYDVHEEYLAIALPSWRWGGFG
                     LSYRSFGVDGIEQRDDRNTLLASDLTSSESEIAVGFGRAFGGAWSVGGTAKIQSQSVA
                     GASGSGLGLDAGVTVQPLLVAGLGVPWADRVSFGLAVRNLIEPSIRLDEASVPDPSVV
                     RTGLAYRSRWTQTAIELEHPRGVGTRLRFGTEFTPHAMLSLRGGWNGRGSVFGAGVRW
                     QSLALDYAFEDAGVGTIHRIGVTLATGRTVAENRAAALRADEERIQTRLSEAFDQIQR
                     ERVDSLLARAEAARAAERYDDALDVLGAVATLAPGDPRAVDLERRCLRESAARLERSG
                     DFTAAALAYGRVLAKDPADSLALVARARCRAESDRRSARTSEIRDRFAQALDAFAAND
                     LAAARSGFLAILAVQPADQEASGMLRRTEQAIARQTDILVREARRAIDAGRLAEADQK
                     LAQARALDPRADGLSVATLALTQARQLALAPSKTPPPSPDARPHAPARPRPTLSRRDL
                     DALYRRGLAAMEQHRPDDALRYWEIVWSADSTYARVGEYLKREYLMRGMNSFAAGRLE
                     EAVALWEKVLVVDPDDARAKGYIARAEKQLARTRELLGEGP"
     gene            complement(9880..17730)
                     /locus_tag="VFR25_10875"
     CDS             complement(9880..17730)
                     /locus_tag="VFR25_10875"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET9941590.1"
                     /translation="MVRRSRFALILLAILWPALSSAGTFTVTVQSRSTNATDSYLSQE
                     NVTENNGSKPELRIKGTAGTKNRHVVFDAALPTLTGKTVLQAWFRMTQSGANSPTPID
                     ARIYPLTESWAESDVTWKNRNLLFGWSTAGGTSGPYWTGRALLSDAGTNGQASWQVGP
                     IVNAWLNGDLAKNGFVIKPEREGSDREVVFRSSEYTNVATATPQLVITYTDEPPAVRS
                     GWAEVQPKAVREGATDTPLTFWLDLDASGASPSGAATGVDLVNVLHNGTIQVTAVDAV
                     LVGGIPVDPAQILWFDNGTSFTLRVPRIHLNAKVEVRCRVSVLAAATTNDLDLPISVD
                     DTSTPGASLQSLWSGNADGTLGNGDDWTLAVTDDPPKVIDLVPDNASVVSRMCTSFQL
                     FGVDAFGNRFGVVADSMSVIPASAGTASKDGTFCAAQPGQAKIVAYAGSLRDTSSVTV
                     TPALVPVISQIVLRTKAGVATTTLVPRDTMLVDVAVSDGDGFRDVTGLDVTLHYQGHA
                     SDSQSPAYRGAYSWRRAGNTWTLVDPAGSSWGVALGRSGVDTTTNATSAQTLRLGFVP
                     GRIARAGSGVWTISVNVFSGTPPDTESAAVNGLSCASRLEIAAVDSAGAFAPAPAGSS
                     AQPLATPADGNLAVAIWSNAPYNLQGAARDFVGIAAPVDTIQAEANTLAWAFRSDGAA
                     GGRLDTLLAALAPPGSALTTEEPETRGLYLWMDYPIVPAQDYAGGVRLRAQASDGSSA
                     STIVDVPLTASVIGAGQAAHSALAEILPHAVQAGAVSQVFTVYVLPSIVAGDAGVDRV
                     LIHLPKGYGAPIVRAVRVAGLPAPYSDASIAGTAEAKLGTAVQSSSLIEVQFRATVPS
                     ELDLAGSDFVTLFDNSGTSVAPQVAAQGNANGIADGDTWRVTVGPGPVARITVKPDGA
                     VCWRDSTLAFAATTEDAFGNPTGGSIGWTALGGVGTIGATTGVFTGTAEGTGLVVAES
                     GGVRDTASVIVRPVRALRVVSVAGPDSLVQGRLGAPFQVLLANPGRDSVSVDLVSLVF
                     TRFIRHDADGDYSVSVTLPLPRSLASGESLSVPLSVNVGRQAQTGPIVVDGSASAVEV
                     ASGIRVSDDGASITHAALVTPGGFTVAAVQTGATVRPGARSVPLLAVTVANQEGSARS
                     LVSLDVKNATAGPGTKDQLDSELGDLTLYLDDGDGVLDPARDPKQFATVAVDGSVHFT
                     PVHVDVPALGSVRLFVAADVSLGARDGDALDLEIADSTSVVLTPSSFARNAWPLRPVG
                     SFPIDGLVAQEIAVHPIPPGNLLAGSTRALALDVTLPPNGYQPDRLLRLSVANSGTAE
                     AGTDVTRMNAWADDGDGVFDPARDRALGELVNTGDRWQRTGLSEVVPVSGLRLFVSVD
                     LADLALDGRTVLLGLPQGTDGGVGMESGDSGPLDQRVENPFALPIRTTDRVTVAAAAL
                     GSAAVHPGDHDAPLLHLIVTNSYTTARTLGRLAVENATSGSGSRLELDREIQSLTLRE
                     DGDADGVLGDLASDPAIGVGSFSNGVVTFTGLSWVLPAGGSRHLFVTADVSPRDAADG
                     DVLGARVSDPLAMHFEEPVSVTGAWPVTSNAQLTVDGMSAAQITNWGAPGASLGPGEG
                     PRLALDVTIPRNGYENDELRSVRIVNLGTAGAAELAELHLWRDGGDGVFTPSSGDDQD
                     LGPLTRTGSEWRSTLLSEALGAAGARLFASVTTTAGPSDSATVDLALPIGAIENLSGN
                     DGPRDGLVANPQPLVISTSPLLATLETETRASTVGQTLRLRMIVRNAGNETVQAIAPS
                     MPAISGSGTTSLQTGPSPGTMTLAPAQSDSFVWVYLASGPGDVQFRADAAGIGTLSGL
                     VRRAVEVSSNGHDILIPVADAGLVPVQSMPLEVSRGQAGIVPFSLTFTNQGGTGASTV
                     SLRGLRIRVEDGNGAPIVPADLITSVVVAEGTNVYLATSTIPGTGSDLDLTFDAPAYV
                     TPEQPTTLSFRLDVAASATAPSFRVAIVDSTDITAEDRTSGAPVRIRLETGSFPLRSG
                     TARLVAEPTELQISDVPSSEQRVAAGSMDVPLLGLQLRNSGIDGVTSDVRVGSIAVTV
                     TDTTGTPIPSPHDWLDRLVVRTATQTLAARIVGGADGPDVTLALAPPLTCPVNAPVDL
                     TILGDVGDSAAVGAFSIRLGSPATIQAWDANTQDTVIVTYASSPIAGAPTIVERAAAS
                     VRVRGVPLFPATLRVGDTDQAALDVVLRHPDGAGTARVRIDSVAVACSDELRRPLAPG
                     TYLARLRVLVNGVERVVQNAFPPSGAVSLPLGGILLAPGDTATIRVLADIAATAPAGY
                     LELSTPIAGLAVFDANSGARLTPLPEPGAVLPPVSGLARLEPPPRELVTGLESRMPAV
                     LAGDGGEVAAGILRLTNTASAGSGGVRLDRLVVRAADRDFAAIAIGSAATRVLAYIDG
                     VLWAESAALTPDSTTASLPADSLLLVPPGGTVEVELRVASAGISPGPTLRLGLDESGI
                     GVVQPGSAVLAIQVRPEPGHSFPLWTESGTVGAATLEASYSNFPNPFAAGREATSFVY
                     YLRADGRVSLRIFTPSGDRVAEVVRDEARSAGLHQADAWTGRNGAGDLVRNGVYLAEL
                     LARYTDGRTERVVRKVAVVR"
     gene            complement(17913..19394)
                     /locus_tag="VFR25_10880"
     CDS             complement(17913..19394)
                     /locus_tag="VFR25_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9941591.1"
                     /translation="MRFTSDPVQFWARVAPDRLALQRGDASWTYRRLNEAVQESADAL
                     TAQGLGAGEHVSLEFAPAHAFSFVIAFHALHRIGLLPVPIGASLPGEERQALRKRAFV
                     DLALTSESSDSERGLEEGPVLGVSVPPTTDVGPRLLRRLETPAALCFTSGTSGEPRGC
                     VLTHGNFFWSALASARNLGVRAGDLWLSCLPLHHVGGLSVLTRSAYYGTGVLLHDRFD
                     PERVLDAIDRQGVTLVSLVPPLLERLLRARGGRMFPTTLRAALIGGGPCPPELLLEAA
                     ELRLRALPTYGLTEAASQVTTLSPRVWPAGLESCGRPLPFVKVEIRGENGLKVSRGAE
                     GEIVVSGPTVMAAYFEEREKDAEAWDGRWLKTGDIGAWDAAGRLVVLDRRSDRMVVGG
                     ENVSPAEVERALRSHPAVLEIAVAGIPAGSWGHEIAAAVVLHPGASLTLNELRDHAME
                     ALANFKLPRRLRIVRELPRGASGKLRRDAFLHLFQEEGAGSST"
     gene            complement(19391..20518)
                     /gene="menC"
                     /locus_tag="VFR25_10885"
     CDS             complement(19391..20518)
                     /gene="menC"
                     /locus_tag="VFR25_10885"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9941592.1"
                     /translation="MRIVRFTLTPYECPLDPPWPAGELRRGVILALEEEGGACGYGES
                     APLPGFGLETLPSSIAALRLSSKYLLGMPRGRYRDAVADLHRLAPVVASPCARHAIDL
                     ALHSLMADDAKKPIARLLGDLNAVDEVPVNAALPRLPREDLVARALAAVAEGYRTLKL
                     KVGGIAIPEDVARVHAVRDAVGREVRLRIDANRAWSEAEATQALHALEDVGLEYCEEP
                     TSDPEALPRVKSAVGVPIAVDESVVDLASVQRILDAADVLVLKPMAVGGLHPARAIAA
                     TARERGVDVVITGMLETPVGRRGALHLAASLGPSLYAHGLATGCTPWPEDSTERSNPS
                     APLSDAGRVLRIGRDDQIPTRMHDWRERNADYRDNLVQEEV"
     gene            complement(20515..21456)
                     /locus_tag="VFR25_10890"
     CDS             complement(20515..21456)
                     /locus_tag="VFR25_10890"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HET9941593.1"
                     /translation="MTDSTELLTEPEAGALPAGHPGRVAAWIHACRIPTLSAAVVPVL
                     VGTVLAWRHGDFALVTAIATLVGAVAIQIGTNLANDLYDYKKGADSPARLGPPRVLAR
                     GWLSVDDVTAGMVAAFGIATASGAYLIAVAGWPILAIGVASIAAGILYTAGPFALAYV
                     GLGDLFVFLFFGLVAVLGTYYAQAGDLDPEAWVAAIPVGTLATAILVVNNLRDIDTDR
                     AAGKLTLAARIGRRATRAEFVTLLLAAFAVPIALWAQGLSSAWVLLPLATTTLAARAL
                     QAVFEKTDGPGLNRTLVDVAQLHLLFGLLFAVGLALG"
     gene            complement(21461..22291)
                     /gene="menB"
                     /locus_tag="VFR25_10895"
     CDS             complement(21461..22291)
                     /gene="menB"
                     /locus_tag="VFR25_10895"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET9941594.1"
                     /translation="MTMTAAIAWTSAKTYDDILYEKADGIAKITINRPDVRNAFRPKT
                     VNEMIDAMDEAREDPEVGVVILTGAGTEAFCSGGDQRVRGNAGYVGDDQVPRLNVLDL
                     QKRIRALPKPVIAMVAGYAIGGGHVLHVVCDLTIAADNARFGQTGPKVGSFDGGFGSS
                     YLARIVGQKKAREIWFLCRQYDAQQALDMGLINTVVPLDRLEEETVAWCREILTMSPL
                     ALRCLKASLNADCDGQAGLQELAGNATLLFYMTEEAQEGRNAYVERRKPDFGKFRRLP
                     "
     gene            complement(22288..>23172)
                     /gene="menH"
                     /locus_tag="VFR25_10900"
     CDS             complement(22288..>23172)
                     /gene="menH"
                     /locus_tag="VFR25_10900"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HET9941595.1"
                     /translation="GCVDRHRVLVDRMSYAVRVHGSAGSPDPVVFLHGFAGSAEDWRD
                     AAEALAGSGYRTLAVDLPGHGSTDAPSDPPCYQPEATARDLATILTSLGVGAAHWVGY
                     SMGARVALRAALDAPSHVLSLTLESASTGIPDPAAREERRRRDDDLANNIEKRGIAWF
                     VPHWESQPIFATQESLHQSRREAQRTRRLLQRPEGLARSLRGLGQGAAPDLTDRLGAI
                     RQPALLLAGALDAAYVANARRISGLLPNASVRIVPEAGHNVHLERPEEFARALLDHLA
                     TVRREHPAGALSSPRSHS"
BASE COUNT         3474 a         8007 c         8234 g         3457 t
ORIGIN      
        1 tgagacccag ttctcccgcc gcggcacggc cgccgacaat ccagtgagcg tccgcgccct
       61 cgcctacatc accgcggggc acgagatcca tcaccgcacc atcttgcgcg agcgctacct
      121 cgggcaggag gctccggccc cgcgcgcctg aggcgcgggc tgcccctcgc cgctcgcggc
      181 gaccaccaac gcctcaaatg aaaggggcgc cctctcgggg cgccccttcg ttgttcctgt
      241 ccgcctcgag gcggccctgc tgagtcgcta cggcttcaac gatacctgcg atcccttggc
      301 gtcatagaag ttgaggagcg gcccctgatc ttccgtgatc atgagctgcg cgcgcggatt
      361 ctccgccgcg tccagcagat cgagagccgg tccatcctgg ccgacgccga ggttcactct
      421 gagcttgccg ttcttgtcga ggatcgtgag ggcggggccg tcatcgcccg cgccgaggat
      481 catctgcgac ttgccctgcg cgttcttcaa gaccagcact tccgcttcga gcttcttcga
      541 cgggttgctg gtcagaccca tgcagagcac gcccgccatg ccgagcagca ccaccgaacc
      601 gagaaccttg aaacgtcgat tctcccgctc cactctctcg agacgctgct ccagagacac
      661 cgattccttt atgtcccgcg tcatgcaccc cccttttctg gacacgcccg tatcgggcaa
      721 cgaaacaacc ctcatctatc ggcagaatcg agagttggct tgagcggaat cacgcccggg
      781 gatgggcaag gtccttcaag tccagggcct tgcgtgccga taactccaga gggacgggga
      841 ggtgtgcgtc ctccggaggt cggatgcgga agggacggta cgttcagctg gtttcctacc
      901 gggtggcggt cgtggtcctc gccatcctga cgggagccct ggccatgcac gagcggggca
      961 cgggagcgtc cgtgtggctc ctgtcggcca tcctgggtgt ggctgccgcg tcggccctcg
     1021 tgtggccgct cgtcgtgccc gcgcgcgacc ggacgatgag cgtctactcg atcggcccgg
     1081 ccttcttcct cgccggcatg ttcctcctgc ctccgcccgc gctcgtcggg atcatcacgt
     1141 tcgcggtcct tctctcgggg ctcattcgcg gagaccgcgc gtatcgcacg gcgttccgcc
     1201 tcgcctcgat ggtgctcgtg ttcgccggat tcgcgatctg gttccgcctc tcgccgcagg
     1261 ggtcggaggt cctgtacaag cctgccgctc gcgcgacgct cgagatggcg atcggagggg
     1321 cggcgctcac agcgctcctg atcttgaaga gcctcgagat tcgcctcgag caagggcccg
     1381 agcacacgcc gcattggggc gccttccaga agccggcgat catggaatcc atgctctgtc
     1441 tcgcgttctc gacgtccacc gtcgtgctcg cgcgcatcca cacgggcttt ctcaccgtcg
     1501 tcggattcca gatggtgatg gtgtggtggt ttctccaccg gtacgctgcc tatacgacag
     1561 ggcttgcgcg cgtcactgac cggaggaagc ggccgcgcgc gatcctgtgg gacgcggcga
     1621 cgcgcgacgc gctacgggtg gggcccttcg cggcgccgct cccatccttg cggcgtttcc
     1681 ccaaggaagc gacagcggcc gaggggcgag aggacgatga cgaggattgg aagtcgggac
     1741 gggccggagg ggcccgctag gagccgaagc gtcgggcgac gtactcctcg acgagcgcga
     1801 ggaattcctc ggcgatgcgt ggtccctgga gcgtggtctt gagcgctcca tcaacgaaca
     1861 cgggggcctt cggatcctcc gccgtgcccg gaagcgagat gccgatgtcc gcgtggcgcg
     1921 attccccggg gccgttcacc acgcatccca tcaccgcgac gcgcaatccc tccacgccgg
     1981 ggtaggcggt gcgccatacc ggcatcttct cgcgcaggcg gagattcacc cgctccgcca
     2041 gctcctggaa gaagatgctc gtcgtgcggc cgcatccggg gcacgacgtg acctgcggct
     2101 cgaagtgccg cagctcgagg gactgcaaga tttgctggca gatccggacc tcctcgcggc
     2161 ggtcgcctcc gggctgcggc gtgaggctca cgcgaatcgt gtcgccgatt ccctcgagaa
     2221 ggagcgtgga gagtcccgcg gtcgacgcga cgactccctt cgcccccatt cccgcctcgg
     2281 tcaatccgag atggagtgga tagtcggtct ccgcggcgag ccggcggtag atcgcgatca
     2341 gatcgcgcac ccccgacacc ttggccgaga gcacgatcgc gtcgtgcggt tgcccgaact
     2401 cctcggcgag cgcggcggag cgcacggcgc tctcgaacat cgcgtcgagc gtgacctccc
     2461 ggtcgtcgcg cggcttggcg ctccgcgcgt tctgatccat gagcgcggtc aggagctccc
     2521 ggtcgagcga tccccaattc acgccgatcc gcaccggctt tccgttggcc accgcgatcc
     2581 ggatgatctg gctgaagtgt tcgtcgtgcc gctcgccgct tccgacgttg cccggattga
     2641 tccggtactt gtcgagggcg cgcgcgcagt cggggaactg gctcaggagg agatgcccgc
     2701 tgaagtggaa gtcgcccacg atcggggccg cgattccggc gtcccgcgtt cgcgcgacga
     2761 tctcgggcac ggagcgcgct gcctccggcg tgttcacggt gacgcgcacg agctcggatc
     2821 cggcctcggc gagcgcgatc acctgggcca ccgtccctgc cgcgtccgcg gtgtcggtgt
     2881 tggtcatcga ctgcaccgcg accgggcggt tcccgccgat cgtcaccgag ccgatccgga
     2941 ctaagaccga ccgccggcgc ggaacggacg tgctgccttg ggaatcgagc ggaatggtga
     3001 tggtcggcat cgacgcgagt ctagcacggc cggcgcgggg cggcgtgcta gactcgccgc
     3061 catgaccacg ccgccgatcg ctgcgacgac gggagcggcg ggcgccgagc cgcaggctcc
     3121 cgccgccccg cgcgtccgca cgatgttcgc gtccatcgcg ggctcctacg atcgcgcgaa
     3181 ccagatcctc tcgctggggc tccaccatcg gtggcgcgag cgcacggtgc gcctgagcgg
     3241 tgcgcggccc ggcatgcgcg tgctcgactg cgcgaccggc acgggagatc tcgcgctcgc
     3301 gttccgccgc gccgtcggcc aagacgggga tgtcgtgggc accgacttct gcgccgagat
     3361 gctcgccctc gctcccggaa aggcggcccg cgcggggctg ccggtgcgat tcgagatcgc
     3421 cgacgtgctc cacctgccct atcccgaccg gtccttcgat atcgcgtcga tcgcgttcgg
     3481 catccggaac gtcgaggatc ccgcgcgcgg gctcctcgag ctggcgcgcg tgacgcgcgc
     3541 cgggggacgc gtcgtcgtgc tcgagttcgg acagccgggg ggagcctggt tcggtccctt
     3601 gtaccgcttc tactcgcact cggttctgcc gcggctcgga ggcgtcatca gcggccagcg
     3661 ctccgcatac gagtacctcg accggacctc ctcgcgcttt ccggcagggg agcggttcct
     3721 cgcgatgatg cgcgacacgg gcgcgtacag cgacgcgcgc gcgcacgccc tcaccggggg
     3781 cgtggcctac gtctacgtcg gagaggtggc gtgagctttc aggcgacgat tcccgtgcgc
     3841 ttccaggacg tcgacgcggc tggcgtgctc ttcttcgggc ggatcttcga ctactgccac
     3901 caggcctacg aggagctgat cggaagcttc ggcgtggatc gcgcgcacta cttcgcggga
     3961 gccgagtacc tggtgccgat cgcgcacgcc cgggccgact accggagccc gatccgcctc
     4021 ggcgagcgcg tccacgtgac gatcgagatc ttgcgggtgg ggcgcgcttc ggtgcggctt
     4081 cgctatcgcg tgacggggcc caaggcggac gatctgcgcg ccgaggtcga gaccgtccac
     4141 gccttcgtcg accgcgccac catgcggccg atctcgattc cggatccgct acgcgggttt
     4201 ttcctcgagc acctcgtccc ggacgctgcc gccgaaggtg tcacgaagta gcgactccta
     4261 tcgagccacg gcgggaagcg agaggcggaa gatggagccg cgcgagagct ggctcgacgc
     4321 cgtgagacgc ccattcatgc ggtgcgccag ctcgagggcg aacgcgaggc ccaggcccag
     4381 tcctcccact tcgcgcgtgg gagagccgtc accctgacgg aacgagccga agatctgggt
     4441 caggcgctcg ggcggaatcc caggcccgtc gtcctccacc tccacgaccg ccgtctcgcc
     4501 atcgaccgcg aggcgtaagg tgatccgcgt gccgcgcggg gtgaacttgg tcgcgttgtc
     4561 cacgagcgcc tcgacgatct ggagcacccg gtgccggtcg aaggccgtcg tgagcggctc
     4621 ctccggcgcc tcgaacacga gctcgcgcaa tccctcggtg attcccggca accactcctc
     4681 gtaataggcg cggaggacga gggtcaggtc gccgcggcga atgtcgagcg gaagggagcc
     4741 ccgggtggcc gcaccgaaat cgagcaagtt ctcgagcatg gccttcagct tcttcgattc
     4801 gtccaggcag acccccgtga actcgcgaac cgtgtccggc agccctccct gatcctcgac
     4861 ggagctgaga tacgcgatga tcacggtgag aggcgtccgg agctcgtggt tcaggttccg
     4921 caggaattcc gacttcaacc ggtccagatc ggtcagctct tcgttcgcca tgcggagctg
     4981 gcggttgttc tcgaggagcc ggttgtagag cgtcgcgttc tgcagcgcga cgccgatcat
     5041 gcccagcgaa gcctggagcg cgtccatctc catggggccg tagggagcgc cgccggcgcc
     5101 gggcccgagc gcgaccaagc cgagcgtctc gccccgggat gtgagcggtg ccaagagctc
     5161 gatttcctcc cgccgcgcga gcgccaccgt ggcgggatgg aggcgcgcct gaaactctcc
     5221 ggctccgagg atgcgccgct cttcgcccag ggcttggagc gcatccggag cgacggtggc
     5281 gcccaatgca tccgccatgc ggcggctcat cccgtgggac cgaaggagaa cgggacgccg
     5341 ctccgatcca tcaggcagga gccagagcgc cgcgcgggaa gcgccgagct gccccatgag
     5401 attgagcagc acgagatcgg cgagcgcata gaggtcgaga ccgaccgtca gctcgcggct
     5461 cagctccgag atcgcgagga gctcgtgttc gcgccgagct gcgagccgga gcgcttcggc
     5521 ggcatcgatg tcaggccggg agggcacgct cggcttcctc ctcgctcgaa tcgatcggca
     5581 ggaacggttc cagattcagg agctgaatca ccgtgagcac gggggtcgac ggggaggcga
     5641 agcggaccgt ccggcccgcc ttgcggaact cctcggccac gctcagaagg gttccgaccc
     5701 cgctcgaagc gatgaactcg acccggctca ggttgacgat gagattccgt ccgctcgcgc
     5761 gcacgtcggc gcactgagcg agcagcgccg gtacgctctt cgcgtcgagc cggcctacga
     5821 cgcgcacgat cactccatgc acgcccgaca ccacttcaag gggttcggcc acgctgtgcc
     5881 tcctctcgtg ggctcgggaa gcgaaggacg gtgatgttgc cttcacgggt tcccgggaaa
     5941 taggccgcct gcctcgtcgc gcggcggatg atctggagtc cgaggccgcg acccttcgac
     6001 cgaacgccgg ggtccccgaa gtcgagatcg gccgcggcgg gcggggcaaa gggagcgccg
     6061 tcatcgcgca cgaggaacat cgtctcccct cgcccgtcgg acacccaacc gaggtcgagc
     6121 gatcgcgacg gatcctcgcg atagccgtgc tccacgatgt tggcgcagac ctcgtacaga
     6181 gcggtcgtga ggagcgcgag ctcccgctcg gacagcgcgc ccgcgggcgg acagctcctg
     6241 agccacgcgc cgattccttc gagcgcctcc atccgcgcgg ggagcgtcca ggtccgtccc
     6301 tggccgcgcg cctggcggag gagctcttcc gagtctccgg gcgacagcgc cccggccgcg
     6361 gtgtttgcgt ccgcctgggt ctcatgggcg atcacgagaa gagtctcgtc gtcgcccggc
     6421 ggagcgttgt cggtccaccc cccgatcgca gagaccacgg cctcgatcac ggaagacgcc
     6481 ccgcctcctg ccgccgcggc caccgtttgc ccgagacgct cgagcccgaa ttgctcgccg
     6541 cgcgcattca tcgattcgtt cacgccgtcc gtgtactgaa ccaggacgtc gcccggttcg
     6601 aggtcgagct tgcggtcctc gagggtcgtc gcgatcgccc caccgcgaac ggctccgatg
     6661 ggaatgccgc gcgtcgcgag aagggccacc gagcggtccc gggcgcggta ccggaggagc
     6721 gggttgtgcc cggcggacgc ataggtgatt cgccctgcgc tcggatcgag cacgccgtag
     6781 aagagcgtca cgaacgagcc cggctgcagc gtttccgcga gcctccggtc gacctgcgcg
     6841 aggagcgacg agggggacgc gatgccgtcc cggtacgcgc gcaggagcga gaacaccatc
     6901 gacatgacga ggcatccggc gagccccttg ccggccacgt ccgccaccac cacgccgatc
     6961 gttccgtcgg gacgatcgaa cacgtcgtag tagtccccgc cgacctcagc cgccggccgg
     7021 tggtatccct ggatcacgaa cgctcccgcg cgggtcgtgc ccttggggag cagggacgtc
     7081 tggatccgct gcgccagctc catctcgtgg gccagccgct cccgctcgac gagatccgcc
     7141 tgtgctcgct tcagctcgag cgccatttga ttgatcgcgg ctgcgagaag gccgagctcc
     7201 gtccgatcgc ggagcacgac cggacgatcc agctcgccgc gcccgatcct ctcgatgcca
     7261 tggcgaagcg cggcgatggg gcggaggagc tgcgagacga gaatcagggg gacggccacg
     7321 gccgcggcga ggaagagcgc gagcaccgcg atctgttgcc gcctcgcgtc ggtgatgacc
     7381 cggtcgaggt agctccgcgg catgcccacg accgcgagcc cgagccgccg accatcggga
     7441 tgggtgacgg gaacggccgc gaggagggtt ccgccgtcct cgccgagcgt ctcgccgcgc
     7501 gccaggcggc gggcgtcctc gatgggcagg agcctggacg gcagcgagta gctctggccc
     7561 agactccgaa cgtccggatg accttgcacg acgccctgat tgtccgcgac gacgatcacg
     7621 gtgagctcgg gctgccgggc gagaagctcc ttggcgagcg gatggagcgt gagctccgga
     7681 tagtcgccga gcatggcccc cgtgcttgcc agcgccagcg tgcgcgcgtc gaggaggaga
     7741 cgggtctcga tctccgcggt gagcgcgcgt cgcgtgttgc gctcggccac ggtgcccacg
     7801 ctcgcgaccg cgcccgcggt gaggagcacc gcggccacgg cgacgatcca ccggatgctc
     7861 cagacgacct tggtcttgcg ctccgtcatg gcccctcccc aagcagctcg cgcgtgcggg
     7921 ccagctgctt ctccgcgcgc gcgatgtagc ctttcgcccg cgcgtcgtcc gggtccacga
     7981 caaggacctt ctcccacagc gcgaccgcct cctctagcct tccggcggca aaggagttca
     8041 tgccgcgcat gaggtactcg cgcttcaagt actccccgac gcgtgcatag gtggagtcgg
     8101 cggaccagac gatctcccag tagcggagcg cgtcgtcggg ccggtgctgc tccatggccg
     8161 caaggccgcg tcggtagagc gcgtccaggt cacggcggga gagcgtggga cgcggccgcg
     8221 ccggcgcgtg cggtctcgcg tcaggcgacg gaggtggcgt cttcgacggc gccagagcga
     8281 gctggcgggc ctgggtcagc gcgagggtcg cgaccgagag tccgtccgcg cgcggatcga
     8341 gcgcccgggc ctgagcgagc ttctggtccg cctccgcgag acggcctgca tcgatcgcgc
     8401 ggcgtgcctc gcgcacgagg atgtcggtct gacgcgcgat cgcctgctcg gtgcgccgga
     8461 gcatccccga cgcttcctga tccgccggct gaacggcgag gatcgcgagg aaccccgacc
     8521 gcgcggctgc gaggtcgttc gccgcgaagg cgtcgagcgc ctgtgcgaag cggtcgcgga
     8581 tctcgctcgt ccgcgccgac cggcggtcgc tctcggcgcg gcaccgcgct cgagcaacca
     8641 aggcgagcga gtcggcggga tccttcgcca gcacgcgtcc gtaggcgagc gctgcggcgg
     8701 tgaagtcccc cgatcgctcg agccgcgccg cactctcacg caggcaccgg cgctcgagat
     8761 cgaccgcgcg cggatcgccg ggcgcgagcg tggcgaccgc gccgaggacg tcgagcgcgt
     8821 cgtcgtagcg ctcggcggcc cgagcggctt ccgctcgtgc gaggagcgaa tcgacccgct
     8881 cccgctggat ctgatcgaag gcctcgctca gccgggtctg gatccgctcc tcgtcggccc
     8941 gaagcgcggc cgcgcgattc tcggccacgg tgcgcccggt cgcgagcgtc acgccgatcc
     9001 ggtggatcgt gccgacgcct gcgtcctcga acgcgtagtc gagcgcgagc gactgccagc
     9061 gcacgcccgc cccgaagacg gagcctcggc cgttccagcc cccgcgcagg gacagcatgg
     9121 cgtgcggtgt gaactcggtg ccgaagcgga ggcgcgttcc gacgccgcgg ggatgctcga
     9181 gctcgatcgc ggtttgggtc catcgcgagc ggtaggcgag cccggtccgg acgacggacg
     9241 gatcgggcac gctcgcctcg tcgaggcgga tcgacggctc gatcagattc cgcacggcga
     9301 gtccgaagga cactcgatcc gcccagggca cacccaagcc cgcaaccagg agcggctgga
     9361 ccgtgacgcc ggcgtccagg ccgaggccgc tccccgacgc acccgcgacg ctctggctct
     9421 ggatcttggc ggtcccgccg acggaccagg caccgccgaa ggcgcgtccg aatcccaccg
     9481 cgatctccga ctccgacgac gtgagatccg atgcgaggag cgtgttgcga tcgtcccgct
     9541 gctcgatccc atccacgccg aacgagcgat aggacaggcc gaagccgccc catcgccagc
     9601 tcggcagcgc gatggcgaga tactcctcgt ggacgtcgta gccgtactgg aaccgccccg
     9661 cctggatctc gcgccgcggc acgagcccga ggcccgcggg attccaggcc atcgcgcttg
     9721 cgtccgacgc gagcgccgcg tacgcacccc cgagcgcgat ggcacgcacg cccgtgccgg
     9781 tggcgaagat cgattgcgtg ccgccgtcgt cctcacctcg tgcgggcggc gcggcgagaa
     9841 cggtgagcgc tgcgatccac gcgatgacga agcgccggat catcgcacca ccgccacttt
     9901 gcggaccacg cgctccgtcc ggccgtctgt gtagcgcgcg aggagctccg ccagatagac
     9961 accgtttcgc accaggtcgc cggcgccgtt ccgccccgtc caggcgtcgg cctggtggag
    10021 cccggccgag cgcgcctcgt cgcgcacgac ctccgccacg cgatcgccgg acggagtgaa
    10081 gatgcggagg ctcacgcggc cgtccgcgcg caggtagtac acgaacgacg tggcctcgcg
    10141 ccccgccgcg aacggattcg ggaagttcga gtagctcgcc tcgagcgtgg ccgcgcccac
    10201 cgtgcccgac tcggtccaga gcgggaagga gtggccaggc tcgggacgga cctggatggc
    10261 gaggaccgcg cttccaggct gcacgacgcc gatgccgctc tcgtccagac cgagcctgag
    10321 cgttggtcct ggggagatcc cggcagatgc cacgcgaagc tctacttcga ccgtgccgcc
    10381 gggcggcacg aggagaaggc tgtccgccgg aagcgacgcc gtcgtggaat cgggcgtgag
    10441 cgcggcgctc tccgcccata ggacgccgtc gatgtaggcc aggacgcggg tggccgcgct
    10501 cccgatcgcg atggcggcaa agtcgcgatc cgcggcgcgc accaccaggc gatccagtcg
    10561 cactccgcct gagccggccg aagccgtgtt ggtcagtcga aggattccgg ccgccacctc
    10621 gcccccgtca ccggcgagaa ccgccggcat gcggctctcg aggccggtca cgagctcgcg
    10681 tgggggcggc tcgaggcgcg cgagacccga caccgggggc agcacggcgc cgggctcggg
    10741 cagcggcgtg agccgggctc cggagttggc gtcgaacacg gcgagccccg cgatcggtgt
    10801 gctgagctcg aggtatcccg cgggcgcggt cgcggcgatg tcggccagca cgcgaatcgt
    10861 cgccgtgtct cccggtgcga ggaggatccc gcccagcgga agcgacacgg ccccagaggg
    10921 cgggaaggcg ttctggacca cgcgctccac gccgttcacg agcacgcgga ggcgcgcgag
    10981 ataggtgccg ggcgcgagcg ggcgccggag ctcgtccgag catgcgactg cgacgctgtc
    11041 gatgcgcacg cgcgcggttc ccgctccgtc cggatggcgg agcacaacgt cgagcgcggc
    11101 ttggtcggtg tcgccgacgc gcaaggttgc ggggaagagc ggcacgccgc gcacgcggac
    11161 ggatgcggcc gcgcgctcca cgatcgtggg agcgccggcg atcggactcg atgcgtacgt
    11221 cacgatgacc gtgtcctgcg tgttggcgtc ccaggcctgg atcgtggccg gagagccgag
    11281 ccggatcgag aacgccccga cggcggcgct gtccccgacg tcgcctagga tcgtgagatc
    11341 gaccggcgcg ttcacgggac acgtgagcgg cggggcgagc gcgagggtca cgtccggtcc
    11401 gtcggcgccg cccacgatcc gggccgccag cgtctgggtc gcggtccgga ccacgagacg
    11461 atcgagccag tcgtgcggcg acggaatggg agtgccggtc gtgtcggtca ccgtcaccgc
    11521 gatcgacccc acacgcacgt cggacgtgac gccgtcgatc cccgagttcc ggagctggag
    11581 accgagaagg ggaacatcca tggagccggc agcgacgcgc tgctcggagg atggaacgtc
    11641 ggagatctgg agctcggttg gctcggccac gagacgcgcg gtcccggagc ggagcgggaa
    11701 cgatcccgtt tcgagccgga tccggaccgg cgctccgctc gtccgatcct ccgcggtgat
    11761 gtcggtgctg tcgacgatgg cgacccggaa gctcggcgcc gtggccgaag cggccacgtc
    11821 gaggcggaag ctcagcgtgg tcggctgctc gggcgtgacg taggccggag cgtcgaaggt
    11881 aaggtcgagg tccgatcccg tccccgggat cgtcgacgtc gcgagataga cgttcgttcc
    11941 ctcggccacc acgaccgagg tgatgagatc ggccgggacg atcggggctc cgttcccgtc
    12001 ctcgacgcgg atgcgaagcc cacgcaggct gacggtcgag gcgccggtgc cgccctggtt
    12061 cgtgaaggtg aggctgaagg gaacgatgcc cgcctggccg cgactcactt ccagcggcat
    12121 cgactggacc gggacgagcc ccgcgtccgc gacggggatc aggatgtcgt gtccgttcga
    12181 cgagacctcg accgcgcgtc gcacgagacc gctcaacgtt ccgattcctg cagcgtccgc
    12241 ccggaactgc acgtcgccgg gccccgaggc gagatagacc cagacgaagc tgtccgactg
    12301 ggcgggagcg agagtcatcg tgcccgggga gggtcccgtc tggagcgagg tggtcccgct
    12361 tccggagatc gccggcatcg agggggcgat cgcctggact gtctcgttcc cggcattgcg
    12421 cacgatcatg cgcagccgga gcgtctgccc gacggtgctc gccctcgtct cggtctcgag
    12481 cgtggcgagg agcggggagg tgctgatcac gagcggttgc ggattggcga cgagcccgtc
    12541 tcgaggtccg tcgttgccgg acaggttctc gatggcgccg atcggaagcg cgaggtccac
    12601 ggtggcggag tccgacggcc ccgcggtcgt cgtcacggac gcgaagaggc gcgccccggc
    12661 ggcaccgagc gcctccgaga ggagcgtgct ccgccactcg gagccggtgc gggtgagcgg
    12721 tcccaggtct tgatcgtcgc ccgagctggg ggtgaagaca ccgtctccgc cgtcccgcca
    12781 caggtgcaat tccgcgagtt ccgccgctcc ggcggttccc agattgacga ttcgcaccga
    12841 gcggagctcg tcgttctcgt atccgttgcg agggatcgtg acgtcgagcg cgaggcgcgg
    12901 accttcgccc ggtccgaggc tcgcgcccgg agcgccccag ttggtgatct gcgccgcgct
    12961 catgccgtcg accgtgagct gcgcgttcga cgtgaccggc cacgcgccgg tcaccgacac
    13021 cggctcctcg aagtgcatcg cgagcggatc cgagacgcga gcgcccagca cgtctccgtc
    13081 cgctgcatcc ctgggcgaca cgtcggcggt gacgaagagg tggcgcgagc cccccgccgg
    13141 aagaacccag ctgagccccg tgaacgtgac gacaccgttc gagaacgaac cgaccccgat
    13201 ggccgggtcg gacgcgagat cgccgaggac tccgtctgcg tcgccgtcct cccgaagggt
    13261 cagcgactga atctcgcggt cgagctcgag gcgcgagccg ctcccggacg tggcgttctc
    13321 gaccgcgagc cgccccaggg tccgcgcggt cgtgtagctg ttcgtgacga tgagatggag
    13381 cagcggcgcg tcgtggtcgc cgggatggac ggccgcggag ccgagcgccg ccgccgccac
    13441 ggtcacgcgg tcggtcgtgc ggatcggcag cgcgaacgga ttttcgactc gctggtccag
    13501 cgggcccgag tccccgctct ccatgcccac cccgccatcc gttccctgcg gaagtccgag
    13561 cagcaccgtc cggccgtcca gcgcgagatc ggcgagatcc acggacacaa agaggcggag
    13621 cccgctgacc gggacgacct cggagaggcc ggtgcgctgc cagcggtcgc ccgtgttcac
    13681 gagctcgccg agcgcgcggt cgcgcgcggg atcgaacacc ccgtcgccgt cgtcggccca
    13741 cgcattcatc cgcgtgacgt ccgttccggc ttccgccgtg ccgctgttgg cgacggagag
    13801 gcgcaggagc cgatccggct gatatccgtt cggaggcagc gtgacgtcca gggcgagcgc
    13861 ccgggtcgag cccgcgagca gattgccggg cggaatcggg tgcaccgcga tctcttgcgc
    13921 cacgagcccg tcgatgggga agctcccgac cggcctgagc ggccacgcgt ttcgagcgaa
    13981 gctcgaaggc gtgaggacga ccgaggtcga gtcggcgatt tcgagatcga gcgcgtcgcc
    14041 gtcgcgcgcg ccgagcgaga cgtccgccgc cacgaacagg cgcacgctgc cgagcgccgg
    14101 cacgtcgacg tgcaccggcg tgaagtggac gctgccgtcg acggcgaccg tcgcgaactg
    14161 cttggggtcg cgggcgggat cgagcacgcc atcgccgtcg tcgaggtaga gcgtgaggtc
    14221 gcccagctcg gagtcgagct gatccttcgt cccaggccct gcggtcgcgt tcttcacatc
    14281 gagcgaaaca agggatcgcg ccgatccttc ctgattcgca acggtgaccg cgagaagcgg
    14341 cacactgcgc gcgcccggcc tcaccgtagc cccggtctgg accgcggcca cggtgaaccc
    14401 gccgggggtc accagggcgg cgtgcgtgat ggaggcgccg tcgtccgaca cgcggatccc
    14461 gctcgcgacc tcgaccgcag aggcgctgcc gtcgacgacg atcggcccgg tctgcgcctg
    14521 gcgcccgacg ttcacggaga gcggcacgga gaggctctcc ccactcgcga gggaccgcgg
    14581 caggggaagc gtcaccgaga ccgagtagtc gccgtccgcg tcgtggcgga tgaagcgcgt
    14641 gaagacgagc gagaccaagt ccacggagac ggagtcccgt ccgggattgg cgaggaggac
    14701 ctggaacggg gcgccgagcc gcccctgcac gagcgagtcg ggtcccgcaa cggacaccac
    14761 gcggagcgcg cgaacggggc ggacgatcac ggaggccgtg tcccgcacgc ctcccgattc
    14821 ggcgacgacg agtcccgtgc cctccgcggt tcccgtgaac actcccgtgg tcgcgccgat
    14881 cgttcccacg cccccgagcg cggtccagcc gatcgatcca ccggtcgggt ttccgaaagc
    14941 gtcctccgtc gtagcagcga atgcgagtgt gctgtcgcgc cagcagacgg cgccgtcggg
    15001 cttcacggtg atccgcgcca cggggcccgg tccgaccgtg acccgccagg tgtccccgtc
    15061 cgcgatgccg ttcgcgtttc cttgcgccgc gacctgggga gccaccgacg tgcccgagtt
    15121 gtcgaagagc gtcacgaagt cgcttccggc cagatccagc tcgctcggca ccgtggcgcg
    15181 aaactgcacc tcgatgaggg aggagctctg gaccgcggtg ccgagcttcg cctccgcggt
    15241 gccggcgatg ctcgcgtccg agtacggcgc gggaagtccc gcgacgcgca cggccctgac
    15301 gatgggcgcg ccgtagccct tggggaggtg gatcagcacg cggtccacgc cggcgtcgcc
    15361 cgcgacgatg ctcggcagga cgtagacggt gaagacctgg ctcaccgccc cggcctgaac
    15421 cgcgtgcggc aggatctccg cgagcgcgga gtgcgcggcc tgaccggcgc cgatgaccga
    15481 ggcggtcaag ggcacgtcga cgatcgtgga cgcgctgctg ccgtcggacg cctgcgcccg
    15541 gaggcggacg ccccccgcgt agtcctgcgc cgggacgatg gggtaatcca tccagaggta
    15601 gagtccgcgc gtctcaggct cttccgtagt cagcgcggat ccgggcggcg cgagcgcggc
    15661 gagcagcgtg tcgaggcgtc ctcccgcggc tccgtccgat cggaatgccc aggcgagcgt
    15721 gttcgcctcg gcttggatcg tgtccacggg ggccgcgatg ccgacgaagt cgcgcgccgc
    15781 tccctggagg ttgtagggag cgttgctcca gatcgcgacg gcgagattcc cgtccgccgg
    15841 agtggcgagc ggctgggcgg aggatcccgc gggggccggt gcgaaggcgc ccgcgctgtc
    15901 gacagcggcg atctcgagcc tggaggcgca cgagaggccg ttgacggcgg cgctctcggt
    15961 gtccgggggc gtgccgctga acacgttcac cgaaatcgtc cagacgccgc ttccggcgcg
    16021 cgcgatgcgt cccggaacga agcccagccg gagcgtctgc gcgctcgtgg cgttcgtcgt
    16081 cgtatcgacg ccggatcggc ccagtgcgac gccccaggac gagcccgcgg gatcgaccag
    16141 ggtccacgtg tttcccgccc tccgccacga gtaggcgcct cggtacgcgg gcgactgact
    16201 gtcggacgcg tgtccctgat agtgcagcgt gacgtcgagt ccggtgacgt cccggaagcc
    16261 gtcgccatcg ctcacggcga catcgacgag catcgtgtcg cgagggacga gcgtcgtcgt
    16321 ggcgacgccg gccttcgtgc gcagcacgat ctgggagatg acgggcacga gcgcgggagt
    16381 caccgtgacg ctcgacgtgt cgcgcagcga tcccgcgtac gcgacgatct tggcctgccc
    16441 gggctgggcg gcgcagaagg ttccgtcctt ggacgcggtg ccggcggacg cgggaatcac
    16501 gctcatgctg tcggccacga ccccgaaccg gtttccgaac gcgtccacac cgaagagctg
    16561 gaaggacgtg cacatccggc tcacgaccga cgcgttgtcg ggcacgagat cgatcacctt
    16621 gggcgggtcg tcggtgacgg cgagggtcca gtcgtcgccg tttccaaggg tgccgtccgc
    16681 gttgccggac cagagggact ggagcgacgc ccccggcgtg gaggtgtcgt cgaccgagat
    16741 cggcaaatcg agatcgttgg tcgtcgcggc ggcgagcacg ctcacgcggc agcgcacctc
    16801 gacctttgcg ttcagatgga tccgtgggac gcgcagcgtg aacgaggtgc cgttgtcgaa
    16861 ccagaggatc tgggccggat cgaccgggat gccaccgacc agcaccgcgt ccacggcggt
    16921 gacctggatc gtaccgttgt gaagaacgtt gacgagatcg actccggtcg cggcgccgct
    16981 cggcgacgct cccgacgcgt ccagatcgag ccagaacgtg agcggcgtat cggtcgctcc
    17041 ttcgcgcacg gctttcggtt ggacttccgc ccatccgcta cggaccgcgg ggggctcgtc
    17101 ggtgtaggtg atcaccagct gcggcgtggc cgtggcgacg ttggtgtatt cgctcgaccg
    17161 gaacacgact tcgcgatcgc tgccctcgcg ctcgggcttg atcacgaatc cgttcttcgc
    17221 gagatcgccg ttcagccacg cgttgacgat cggcccgacc tgccagctcg cctgtccgtt
    17281 ggtcccggcg tcgctcagga gcgcgcgtcc ggtccagtag gggcccgacg tgcctcccgc
    17341 cgtggaccag ccgaagagga ggttccggtt cttccacgtc acgtcggatt cggcccagga
    17401 ctcggtgagg ggatagatcc tcgcgtcgat cggcgtcggg ctgttggcgc cgctctgggt
    17461 catgcggaac cacgcctgga ggacggtctt gccagtgagc gtggggagcg ccgcgtcgaa
    17521 gacgacgtgg cggttcttcg tccctgccgt ccccttgatg cggagctcag gcttgctgcc
    17581 gttgttctcg gtgacgtttt cctggctcag gtacgaatcg gtcgcgttcg tggatctcga
    17641 ctggaccgtg accgtgaacg tgcctgcgct cgagagcgcg ggccacagga tggcgagaag
    17701 gatgagagcg aagcgtgagc ggcggaccac gaatcctccg tgcgaacacc gggtcggggc
    17761 gtccttggag aattcggcag atcaggggta tttcttgatg ccggttggac ttggaggagc
    17821 gcgcctggct cagagcgcgc gtgttgcaaa cgtcgtgttc cgtgagggtt ggccctctgc
    17881 ctggcggcga ccggagaacg cgccggccca ggtcaggtcg agcttccggc cccttcttcc
    17941 tgaaagagat gaaggaacgc gtcgcgccgg agttttcccg aggcgccccg cgggagctcg
    18001 cgaacgatcc gaagccgtcg tgggagcttg aagttcgcga gtgcctccat ggcgtggtcc
    18061 cggagctcgt tcaacgtcag cgacgcgccg ggatggagca ccaccgctgc ggcgatctcg
    18121 tgcccccacg atcccgccgg aatgcccgcc accgcgatct cgaggacggc ggggtgcgat
    18181 cgcaacgctc gctcgacctc ggcgggggac acgttctcgc cgccgaccac catgcgatcc
    18241 gacctccggt cgagcacgac gagacgcccc gcggcatccc atgcgccgat gtctcccgtc
    18301 ttgagccaac ggccgtccca cgcttccgcg tccttctcgc gctcctcgaa gtaggcggcc
    18361 atcacggtgg gcccgctcac cacgatctcg ccttcggcgc cgcgcgacac cttgagtccg
    18421 ttctcgcccc ggatctccac cttgacgaat ggaagcggcc gtccgcacga ttcgagtccg
    18481 gccggccaca cgcgcggcga gagcgtggtc acctgcgacg ccgcctcggt gagtccgtac
    18541 gtcggaagtg ccctcaggcg aagctccgcg gcctccagga ggagctcggg cgggcaagga
    18601 ccgccaccga tgagcgcggc gcgcagagtc gtcgggaaca tgcgccctcc gcgcgcgcgg
    18661 aggagccgct cgagaagcgg cggcacgagc gagacgagcg tgaccccctg ccggtcgatc
    18721 gcgtcgagca cgcgctcggg atcgaagcgg tcgtgaagga gcaccccggt cccgtaatac
    18781 gcgctccgcg tgaggacgga gagtccgccc acgtggtgga gcggcaggca ggagagccag
    18841 agatcgccgg cgcgcacgcc gagatttcgc gccgacgcga gcgcgctcca gaagaagttc
    18901 ccgtgtgtga gcacgcagcc gcgcggctct cccgaggtgc ccgaggtgaa gcagagcgcg
    18961 gctggcgtct cgagacggcg caggagtcgc ggtcccacgt cggtcgtcgg cggcacgctc
    19021 acgccgagca cggggccttc ctccaggccg cgctcgctgt ccgaggactc gctcgtgagc
    19081 gcgagatcca cgaaggcccg cttccggagc gcctgtcgct cctccccggg cagcgatgcg
    19141 ccgatgggga cgggcaggag accgatccga tgcagcgcgt gaaacgcgat gacgaagctg
    19201 aacgcgtgcg cgggggcgaa ctcgagcgac acgtgctcgc ccgcgccgag tccctgcgcg
    19261 gtcagcgcgt cggccgactc ctgaaccgcc tcgttcagtc gccggtaggt ccaggacgca
    19321 tccccgcgct ggagcgccaa gcgatcggga gcgacgcgcg cccagaactg cacgggatcc
    19381 gaggtgaatc tcacacttcc tcctggacca ggttgtcccg atagtcggcg ttccgctcac
    19441 gccagtcgtg catgcgggtt gggatctggt cgtcacgtcc gatcctcaag actcggcccg
    19501 cgtccgagag cggagcggaa gggttcgagc gttcggtgga gtcctcgggc cacggcgtgc
    19561 accccgtggc caggccgtgt gcgtagaggc tcggccccag ggaagcggcc agatgaagcg
    19621 cgccgcgtcg cccgaccggg gtctcgagca tcccggtgat gacgacgtcc actccgcgct
    19681 cccgcgccgt ggcggcgatc gcgcgcgcgg gatgaagacc cccgacggcc atgggcttga
    19741 ggacgagcac gtccgccgcg tcgagaatgc gctgtaccga ggccaggtcc acgacgctct
    19801 cgtccacggc gatcggaacg cccaccgcgc tcttcacccg cgggagggct tcgggatcag
    19861 aggtgggctc ctcgcagtac tcgagaccga cgtcctcgag tgcgtggagc gcctgggtgg
    19921 cttcggcctc gctccacgcg cggttggcgt cgatgcggag ccggacctcg cggcccactg
    19981 cgtcgcgcac ggcatgcacc cgcgcgacgt cctccgggat cgcaatcccc ccgaccttga
    20041 gcttcaacgt gcgatagcct tccgcgaccg ccgcgagcgc tctagccacg aggtcctcgc
    20101 ggggaagtcg tgggagcgcc gcattgaccg gaacctcgtc gacggcgttg agatcgccga
    20161 gcaggcgggc gatcggcttc ttcgcgtcgt cggccatgag actgtgaagc gcgagatcga
    20221 tggcgtggcg cgcgcatggc gacgccacga cgggggcgag gcggtggaga tcggccaccg
    20281 cgtcccggta tcgccctcgc ggcatcccga ggaggtattt cgacgagagg cggagggccg
    20341 cgatgctcga aggaagggtc tcgaggccga agccgggaag cggagcgctc tcaccatatc
    20401 cacaggcgcc cccctcctcc tcgagcgcga ggatgacccc gcgcctgagc tcgcccgcgg
    20461 gccacggcgg atccaggggg cactcgtacg gagtgagcgt gaaccggacg atcctcatcc
    20521 gagcgcgagc cccacggcga agaggaggcc gaagaggaga tggagctggg ctacgtccac
    20581 gagcgttcgg ttgagccccg gtccgtccgt cttctcgaag acggcttgga gcgcgcgcgc
    20641 ggcgagcgtc gtcgtcgcga gcggaagcag cacccacgcc gaggagaggc cctgcgccca
    20701 gagcgcgatc ggcacggcga acgcggccag gaggagcgtg acgaactcgg cgcgcgtggc
    20761 gcgccggccg atgcgcgcgg cgagcgtgag ctttcccgcg gcgcggtcgg tgtcgatgtc
    20821 gcgcagattg ttcacgacga ggatcgcggt cgcgagcgtt cccacgggaa tggccgcgac
    20881 ccatgcttcg ggatcgagat cgcccgcctg cgcgtagtac gtgccgagca ccgcgacgag
    20941 accgaagaag aggaacacga agagatctcc gagcccgacg tacgcgagtg cgaacggacc
    21001 ggcggtgtag aggatccccg ccgcgatgga ggcgacaccg atcgcgagga tcggccaccc
    21061 ggcgacggcg atgagatagg cgccggaggc ggtcgcgatg ccgaaggcgg ccaccatgcc
    21121 ggccgtcacg tcgtcgacgg acagccaccc gcgtgcgagg acgcgcggcg ggccgagccg
    21181 cgcgggcgag tcggccccct tcttgtagtc gtagagatcg ttcgcgaggt tcgtcccgat
    21241 ctggatcgcg accgcgccca cgagggtcgc gatggcggtg accagcgcga agtcgccgtg
    21301 acgccacgcg agcacggtgc cgacgagaac cggcaccacc gcggccgaaa gggtcgggat
    21361 gcggcaggcg tggatccagg cggcgacgcg gcccggatgc cccgccggga gcgcgcccgc
    21421 ctcgggctcg gtgaggagct cggtggaatc ggtcacgaga tcatgggagg cgacggaact
    21481 tgccgaagtc aggcttccgc cgctccacgt aggcgttccg gccctcctgg gcttcctcgg
    21541 tcatgtagaa gagaagcgtc gcgttcccgg ccaattcttg aagtcccgcc tgtccgtcgc
    21601 aatcggcgtt gagcgacgcc ttgaggcatc ggagcgcgag cggactcatc gtcaagattt
    21661 cgcggcacca ggcgacggtc tcctcttcga ggcgatcgag cggcacgacc gtgttgatta
    21721 gccccatgtc gagcgcctgc tgcgcgtcgt actggcggca gaggaaccag atctcgcgag
    21781 ccttcttctg gcccacgatg cgcgcgaggt aggacgatcc gaatccgccg tcgaacgatc
    21841 ccaccttggg accggtctgg ccgaagcgag cattgtcggc cgcgatcgtg agatcacaca
    21901 cgacgtggag cacgtggccg ccgccgatcg cgtagcccgc gaccatcgcg atgaccggct
    21961 tcgggagcgc gcggatgcgc ttctgcaggt cgagcacgtt gagccggggc acctggtcgt
    22021 cgccgacgta gccggcgttt ccgcggacgc gctgatcccc gcccgagcag aacgcctcgg
    22081 ttccggcgcc ggtcaggatc accacgccga cctcggggtc ctcgcgcgct tcatccatcg
    22141 cgtcgatcat ctcgttcacc gtcttcggcc ggaacgcgtt ccggacgtcg gggcggttga
    22201 tcgtgatctt ggcgatcccg tccgccttct cgtagaggat gtcgtcgtag gtcttcgcag
    22261 atgtccaggc gatcgccgcc gtcatggtca tgagtggctc ctcggggatg agagtgcgcc
    22321 ggccgggtgt tcccgtctca cggtcgccag atggtcgagt agagcccgcg cgaactcctc
    22381 gggccgctcc agatgaacgt tgtggcccgc ttcgggaacg atccgcacgg aggcgttcgg
    22441 aagcaggccg gagatccgcc gcgcgttcgc gacgtaggcg gcatcgagcg cgccggccag
    22501 gagcagcgcc ggctgccgga tcgcgccgag ccggtcggtg aggtcgggcg cggctccctg
    22561 gcccaagccg cgcagcgagc gcgccagccc ctcggggcgc tggaggagcc gcctcgtgcg
    22621 ctgcgcctca cgacggctct ggtgaagaga ttcctgcgtg gcgaagattg gctgactctc
    22681 ccagtggggc acgaaccatg cgatgccgcg cttctcgatg ttgttcgcga gatcgtcgtc
    22741 cctccggcgc cgctcctcgc gcgcggcggg atcgggaatt ccggtcgacg cgctctcgag
    22801 cgtgagcgag agcacatgcg acggcgcatc gagggcggcg cgaagcgcca ctcgagcccc
    22861 catcgaataa ccgacccagt gcgcggcgcc caccccgagc gacgtcaata tcgtcgcgag
    22921 gtcgcgggcc gtggcctcgg gctggtaaca cgggggatcg gatggagcgt ccgtgcttcc
    22981 atgtcccggc agatccacgg cgagtgtgcg gtatccggat cccgcgagcg cctccgccgc
    23041 atcgcgccag tcctcggcgg agcccgcaaa gccatggagg aagacgacgg ggtccgggct
    23101 cccggcgctg ccatgcacgc gcaccgcgta gctcatgcga tccacgagga cgcggtgccg
    23161 gtcgacgcag cc
//
LOCUS       DASTKC010000260         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_2120007, whole genome shotgun sequence.
ACCESSION   DASTKC010000260 DASTKC010000000
VERSION     DASTKC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120007"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFR25_10905"
     CDS             complement(<1..216)
                     /locus_tag="VFR25_10905"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HET9941596.1"
                     /translation="MSFVGRSDAERRAMLERIGVSRDEDLWASIPAEFRVQGPLPLPA
                     PLAEYEIARRFEQWGERNADAGRYACFL"
     gene            complement(213..599)
                     /gene="gcvH"
                     /locus_tag="VFR25_10910"
     CDS             complement(213..599)
                     /gene="gcvH"
                     /locus_tag="VFR25_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9941597.1"
                     /translation="MNVPRELRYTKEHEWIRVEGGEAVVGVTDYAQGELGDVIFVELP
                     AAGSKVTYMKPFGTIDAVKTVSDLFAPVTGEIVAVNGALKDDPALVNRSPYKDGWMVR
                     IRLDSPAEVNALLSAEDYEKTLGAHA"
     gene            complement(596..1729)
                     /gene="gcvT"
                     /locus_tag="VFR25_10915"
     CDS             complement(596..1729)
                     /gene="gcvT"
                     /locus_tag="VFR25_10915"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9941598.1"
                     /translation="MLETASALKRTPFYPRHVALGAKMVPFAGHEMPIQYEGIMAEHR
                     AVRTGVGVFDVSHMGEIRLTGGSAIAFANRLVTNEAGQLPEGKVVYTPMCLESGGIID
                     DLLVYGLGADRLLVVNAANFEKDLAWVQEHCPADVTVHDENAETAQLAVQGPKSEDVV
                     ARLYGERVRALGFYEGFEIGTGDDWTLISRTGYTGEDGFEIYVRAPRALALWDRVFEA
                     GSPEGIRPIGLGARDTLRLEMGYCLYGNDIDETTNPLEAGLAWTVRWDKSEFIGAESL
                     RRFKELGVSRRLQGLVLDGDKIARAGAEVDKDGTNVGKVTSGSMGLSIGRPIAMAYLA
                     REASAIGQTVDVVTRGHAVPAKVTARPMYQHGSVRSPKPRRPQ"
     gene            complement(1798..4650)
                     /gene="uvrA"
                     /locus_tag="VFR25_10920"
     CDS             complement(1798..4650)
                     /gene="uvrA"
                     /locus_tag="VFR25_10920"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9941599.1"
                     /translation="MDEIRVRGAREHNLKNLNLDIPRNALVVLTGVSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLGQMEKPDVDQIEGLSPAISIEQRTAGSNPRSTVATVT
                     EIYDYLRLFFARIGVQHCVNCGRPIRSQTVQEMVDHLLATRQGQRLQILAPVIQGRKG
                     EYRKEMAQWRKLGFVRARIDGAFVELEDEIALDKKKKHTIEIVVDRLVADLERRSRLH
                     ESLETATRLAEGMALVLGAGGEEETLSASAVCPACGLSYEPLQPRSFSFNSPYGACKS
                     CDGLGTTLEIDPDKVVPDRTKSLRDGAVAFWGDPEGTWTKGILAAIAKHLDVSFDTPF
                     GKLPAAAQKAILYGLGDEKLDYKFKMRSGKVWKHQGRFRGVIPEMLRRFKETKSDSLR
                     REIEGCMSKTPCPACGGARLRPESLAVEVGGDSIATWVGRSVEAALAALDRLTLGPRA
                     ASIAGPIVKELRARLEFLLHVGVGYITLDRAASTLSGGEAQRIRLATQIGSRLTGVLY
                     ILDEPSIGLHPRDNRKLLDTLLTLRNLGNSVLVVEHDRETMEAADHIVDLGPGAGRRG
                     GYLVAEGPLAAIEAAPQSLTGQYLSGAREIRVPEMRRPGNGSWIEVVGARQNNLKSIR
                     ARFPLGTLVCVTGVSGSGKSTLVNDILHRALARKLHGALDAPGAHERITGSEAIDKVI
                     DIDQSPIGRTPRSNPATYTGVFGLIRDLFAQVPEAKVRGYQPGRFSFNVKGGRCEACA
                     GDGLVKIEMHFLPDVYVVCDVCRGKRYNRETLEVTYKGKSIADVLEMTVDDALELLGP
                     IPSIRRKLETLAGVGLGYIHLGQSATTLSGGEAQRVKLATELSRVETGRTLYILDEPT
                     TGLHFEDVRFLLDVLDRLVERGNTVVVIEHNLDVIKRADWILDLGPEGGEAGGLIVAE
                     GTPETVVRAPGSYTGEALRPALEAAGRGAAEAEAAV"
BASE COUNT          673 a         1657 c         1576 g          782 t
ORIGIN      
        1 caggaagcag gcataccggc cggcgtcggc gttccgctcg ccccactgct cgaagcggcg
       61 cgcgatctcg tactccgcga gcggcgcggg aagaggaagc gggccctgga cgcggaactc
      121 agcgggaatc gaggcccaga ggtcctcgtc ccgggagacg ccgatccgct ccagcatggc
      181 gcggcgttcg gcgtcgctcc gtccgacgaa gctcacgcgt gcgcccccag ggtcttctcg
      241 taatcctcgg ccgagaggag cgcgttcact tcggcgggcg agtcgagccg gatgcgcacc
      301 atccacccgt ccttgtaggg cgagcggttc acgagcgcgg gatcgtcctt gagcgcaccg
      361 ttcaccgcca cgatctcgcc ggtgaccggc gcgaagagat cgctcaccgt cttcaccgcg
      421 tcgatcgtgc cgaacggctt catgtacgtc accttggagc ccgcggccgg gagctcgacg
      481 aagatcacgt cgcccagctc cccctgcgcg tagtcggtca cgccgaccac cgcctcaccc
      541 ccctcgacgc ggatccactc gtgctccttc gtgtagcgca gctcgcgcgg cacgttcatt
      601 gcggcctcct gggtttcggc gatcgaacgg atccgtgctg gtacatcgga cgcgcagtga
      661 ccttggccgg cacggcgtgg ccgcgcgtga ccacgtcgac ggtctggcct atggccgagg
      721 cctcgcgcgc gaggtaggcc atggcgatcg ggcgcccgat cgagaggccc atcgagccgc
      781 tcgtgacctt gccgacgttg gtcccgtcct tgtcgacctc cgcgccggcg cgcgcgatct
      841 tgtctccatc gaggaccagc ccctggagcc ggcgcgacac gcccagctcc ttgaaccggc
      901 ggagcgattc cgccccgatg aactcggact tgtcccaccg cacggtccac gcgagccccg
      961 cctcgagcgg gttcgtggtc tcgtcgatgt cgttcccgta gaggcagtag cccatctcga
     1021 gcctcagcgt gtcgcgcgcg ccgaggccga tcgggcggat tccctcgggg ctccccgctt
     1081 cgaagacgcg atcccagagc gcgagcgcgc gcggcgcgcg gacgtagatc tcgaacccgt
     1141 cctcgcccgt gtagccggtg cgcgagatga gcgtccagtc gtcgccggtt ccgatctcga
     1201 agccttcgta gaagccgagc gcgcggacgc gctcgccgta gaggcgcgcc accacgtcct
     1261 cggacttggg accctgcacc gcgagctgcg ccgtctcggc attctcgtcg tgcacggtga
     1321 cgtcggccgg gcagtgctcc tggacccagg cgaggtcctt ctcgaagttc gcggcgttca
     1381 cgacgaggag ccggtccgcg ccgagtccat agacgaggag gtcgtcgatg attccgccgc
     1441 tctcgaggca catcggcgtg tagaccacct tgccttccgg gagctgcccc gcctcgttcg
     1501 tgacgagtcg gttcgcgaac gcgatggcgc tgccgccggt cagccggatc tcgcccatgt
     1561 gggagacgtc gaagaccccg accccggtgc ggacggcccg gtgctcggcc atgatccctt
     1621 cgtactggat cggcatctcg tgcccggcaa aggggaccat cttggccccg agggccacgt
     1681 ggcgggggta gaacggagtg cgcttcagcg cggaggccgt ttcgagcatc ctctttcctt
     1741 agggatagat acggcgtcga tcgaagaggg gcaaccttag cacatccccg gtggatttca
     1801 aacggcggcc tcggcctctg ctgctccccg gccggccgct tcgagcgcgg ggcgcagcgc
     1861 ctctccagtg tagctccccg gagccctcac gaccgtctcc ggcgtgcctt cggccacgat
     1921 caggcccccc gcttcgcccc cctcgggacc gaggtcgagg atccagtcgg cccgcttgat
     1981 cacgtccagg ttgtgctcga tcaccacgac cgtgttcccc cgctcgacca gacggtccag
     2041 cacgtcgagg aggaatcgca cgtcctcgaa atggagcccg gtcgtcggct cgtcgaggat
     2101 gtagagcgtg cgcccggtct cgacgcgcga gagctcggtc gcgagcttca cgcgctgcgc
     2161 ctcgccgccc gagagggtcg tcgccgactg tccgagatgg atgtacccga gccccacgcc
     2221 cgcgagcgtc tcgagctttc ggcgaatgga cgggatgggt ccgaggagct cgagcgcgtc
     2281 gtccacggtc atctcgagca cgtccgcgat cgacttcccc ttgtacgtga cctcgagcgt
     2341 ctcccggttg tagcgcttcc cgcggcagac gtcgcagacg acgtacacgt cgggcaggaa
     2401 gtgcatctcg atcttcacga gcccgtcccc cgcgcacgcc tcgcaccgtc cgcccttcac
     2461 gttgaagctg aagcgccccg gctggtagcc gcggaccttg gcttcgggaa cctgcgcgaa
     2521 gaggtcgcgg atcagcccga acacgcccgt gtaggtcgcg ggattcgatc gcggcgtccg
     2581 tccgatcggc gactggtcga tgtcgatcac cttgtcgatc gcctcgctcc ccgtgatccg
     2641 ctcgtgcgct cccggcgcat cgagcgcccc gtggagcttt cgcgccagcg cgcggtgcag
     2701 gatgtcgttc acgagcgtgc tcttcccgga tcccgacacc cccgtcacgc agacgagggt
     2761 tccgagcggg aatcgcgcgc ggatgctctt gaggttgttc tgccgggccc cgacgacctc
     2821 gatccaggag ccgttcccgg gacggcgcat ctcgggcacg cggatctcgc gcgcgccgga
     2881 gaggtactgc cccgtgagcg actgcggcgc cgcctcgatc gcggcgagcg gaccctcggc
     2941 cacgagatac ccgccgcgcc ggcccgcgcc tggtcccagg tccacgatgt gatccgccgc
     3001 ttccatcgtc tcccgatcgt gctcgacgac gagcacggag ttgccgaggt tccgcagcgt
     3061 gaggagcgtg tcgaggagct tccggttgtc ccgcgggtga agtccgatgc tcggctcgtc
     3121 gaggatgtag aggacgccgg tgagccgcga cccgatctgc gtggcgagcc ggatccgctg
     3181 cgcctcgccc cccgagagcg tcgatgccgc gcggtccagc gtgatgtacc cgacgccgac
     3241 gtggagcagg aactcgaggc gcgcccggag ctccttcacg atcggccccg cgatcgacgc
     3301 cgcgcgcggg ccgagcgtga gccgatcgag cgcggcgagg gcggcttcca ccgagcggcc
     3361 gacccacgtc gcgatcgagt ccccgccgac ctcgaccgcc agcgattcgg gccggaggcg
     3421 cgcgccgccg caggcgggac agggtgtttt cgacatgcat ccttcgatct cgcgcctcag
     3481 ggagtcggac ttggtctcct tgaaacgccg gagcatctcg ggaatcacgc cccggaagcg
     3541 gccctggtgc ttccagacct tcccgctcct catcttgaac ttgtagtcga gcttctcgtc
     3601 cccgagcccg tagaggatcg ccttctgcgc cgcggcgggc agctttccga acggcgtgtc
     3661 gaacgaaacg tcgaggtgct tcgcgatcgc ggcgaggatc cccttggtcc acgttccctc
     3721 gggatcgccc cagaacgcga ccgcgccgtc gcggagggat ttcgtccggt cggggacgac
     3781 cttgtccgga tcgatctcga gcgtggtgcc gagtccgtcg cagctcttgc acgcgccgta
     3841 gggactgttg aacgagaagg accgcggctg gagcggctcg taggagagcc cgcaggcggg
     3901 gcacacggcg ctcgccgaca gggtctcctc ctcgccgccc gcgccgagca cgagcgccat
     3961 cccctcggcg agccgtgtcg cggtctcgag cgattcgtgg aggcggctcc gccgctcgag
     4021 atcggcgacg agccggtcga ccacgatctc gatggtgtgc ttcttcttct tgtcgagggc
     4081 gatctcgtcc tccagctcga cgaaggcgcc gtcgatccgg gcccggacga atccgagctt
     4141 ccgccactgc gccatttcct tccggtactc gcccttccgc ccctggatga ccggggcgag
     4201 gatctggagc ctttgcccct gccgcgtcgc gaggaggtgg tcgaccatct cctggacggt
     4261 ctggctccgg atcggccggc cgcagttcac acagtgctgg acgccgatcc gggcgaagaa
     4321 gaggcgaagg tagtcgtaga tctcggtgac cgtggcgacg gtcgaccggg gattgctccc
     4381 cgccgtccgc tgctcgatcg agatggccgg cgagagcccc tcgatctggt ccacgtccgg
     4441 cttctccatc tgccccaaga actggcgggc ataggcggag agggactcca cgtaccgcct
     4501 ctggccctcc gcgtagaggg tgtcgaaggc gagggaggac ttgccggagc cggagacgcc
     4561 cgtgaggacg acgagggcgt tccgtgggat gtcgaggttc agattcttga ggttgtgctc
     4621 gcgcgcgccg cgaacgcgga tctcgtccat gggagccatg aatcttagca cacgcgcgcg
     4681 gcggatcc
//
LOCUS       DASTKC010000261         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1410158, whole genome shotgun sequence.
ACCESSION   DASTKC010000261 DASTKC010000000
VERSION     DASTKC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410158"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /gene="recA"
                     /locus_tag="VFR25_10925"
     CDS             <1..838
                     /gene="recA"
                     /locus_tag="VFR25_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9941600.1"
                     /translation="LTIIAQAQKAGGNAVFIDAEHALDAKYAHRLGVDIESLHVAQPD
                     TGEEALEIAEHLVRSGAVDVIVIDSVAALVPKAEIEGEMGDSHMGLQARLMSQALRKL
                     TGTISKSKTTVIFINQIRMQIGVMFGNPETTTGGRALKFYASVRMDIRRIGAIKEGEA
                     VIGSRVKVKVVKNKVAAPFREAEFDILYNEGISRQGELLDLAIDRNLVQKNGTWFSYG
                     EERIGQGRENARLWLKEHADAASELETKLREAMGLTQAPENGAEPAVATPEARGERRV
                     RI"
     gene            842..1363
                     /locus_tag="VFR25_10930"
     CDS             842..1363
                     /locus_tag="VFR25_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET9941601.1"
                     /translation="MPVRPDRAASEEAAKDAALRMLGRAPRTEREIRDRLLTRGFVPN
                     AVEGALERLRRVSLLDDRAFVRAYLHSEVPRRPRGMRLLAATLKRRGVPPLLLEELDQ
                     LLGEDPDLVERELDREEGRARAALAQIARRAGSRKAGDPRAGQRLMGALVRRGFDWSL
                     VRDLIPSPNETRE"
     gene            1374..>3205
                     /gene="alaS"
                     /locus_tag="VFR25_10935"
     CDS             1374..>3205
                     /gene="alaS"
                     /locus_tag="VFR25_10935"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET9941602.1"
                     /translation="MKRTSSNDLRKLFLDFFIERGHPHLPQAPLVPLDDPTLLFTSAG
                     MVPFKPYFSSPLPPPTRRAVTVQRCLRLTDVEQVGQTVRHATFFEMLGNFSFGDYFKK
                     QAVEWAWEFSTQVLALSPDRLWPSVYKDDDEAFDLWAKHIGLGADRVTRLGEKDNFWG
                     PAGGTGACGPSSEIYWDQGPGTGCQRPECAPGCDCERYLEFWNLVFPQFDQLQSGERK
                     PLANRGIDTGMGLERLAMILQASPSVQDNDLLAPIGDAVRRMGSAADAATDRGRRAVR
                     VITDHARALVFTFAEGVRPSNEGRGYVVRRLLRRAARFGRDVGIQGPFLARLVPTVVQ
                     QMAAYDEYAYLKREESIVADAMREEESRFAETLEQGMARFEETASQLEKRREKIFPGA
                     VAFQLYDTFGFPLDLTVEMAMERGLTVDQPGYETAMQEQRDRARKAGRFETRRADAGP
                     WQALTQGAHSAFTGYDQWALDGVSIRSLRETGTDREVELTLDKTPFYPEGGGQVGDTG
                     WLQGEGARLRVIDTYREGDAILHRAEIVEGAAHAGPWRAVVDAEKRAATQRNHTATHL
                     LHAALRARLGTHLKQAGSLVAPDRLRFDFSHGKALTPEDVHAIES"
BASE COUNT          516 a         1089 c         1155 g          445 t
ORIGIN      
        1 gctcaccatc atcgcgcagg cgcagaaggc aggcgggaac gcggtcttca tcgacgccga
       61 gcacgcgctc gacgcgaagt acgcgcaccg cctcggcgtg gacatcgaga gcctccacgt
      121 ggcccagccc gacacgggcg aggaggcgct cgagatcgcc gagcacctcg tgcgaagcgg
      181 cgccgtggac gtgatcgtga tcgactcggt ggccgcgctc gtgccgaagg ccgagatcga
      241 gggcgagatg ggcgattcgc acatgggcct ccaggctcgc ctcatgtcgc aggcgctccg
      301 gaagctcacc ggcaccatct cgaagtccaa gaccacggtc atcttcatca accagatccg
      361 catgcagatc ggcgtcatgt tcggcaatcc cgagaccacg accggcggcc gcgcgctcaa
      421 gttctacgcg tcggtgcgca tggacatccg ccggatcggc gccatcaagg aaggcgaggc
      481 ggtcatcggg agccgcgtga aggtgaaggt cgtgaagaac aaggtcgcgg cccccttccg
      541 ggaagccgag ttcgacatcc tctacaacga ggggatctcg cggcagggcg agctcctcga
      601 tctcgcgatc gaccggaacc tcgtgcagaa gaacggcacg tggttctcgt acggcgagga
      661 gcggatcggc caggggcgcg agaacgcgcg cctgtggctg aaggagcacg cggatgcggc
      721 ctcggagctc gagaccaagc tccgcgaggc gatgggcctg acgcaagctc ccgagaacgg
      781 ggccgagccg gcggtagcga cgccggaagc ccgcggggag cgccgggtcc ggatctgagc
      841 catgccggtc cggccggacc gggcggcgtc cgaagaagcg gccaaggacg ccgcgctccg
      901 catgctgggg cgggcacccc gcacggagcg tgagatccgg gatcgattgc tgacgcgcgg
      961 attcgtcccg aacgcggtgg aaggcgctct cgagcgcctt cgccgcgtct cgcttctgga
     1021 cgaccgcgcg ttcgtgcgcg cgtacctgca ctcggaggtg ccgcgccggc cgcgggggat
     1081 gaggctcctc gccgccacgc tcaagcggcg cggcgtgccg ccgctcctgc tcgaggagct
     1141 ggaccagctt ctcggcgagg atccggatct ggtcgagcgc gagctcgacc gggaggaggg
     1201 gcgggcgcgg gccgcgctcg cgcagatcgc gcggcgcgcg ggctcacgga aggccggcga
     1261 tccgcgggcc gggcagcgtt tgatgggcgc gctcgtgcgg cgcggattcg actggagcct
     1321 ggtgcgggat ttgatcccgt ccccgaacga aacccgggag tgactcgatc cacgtgaagc
     1381 gaacgtccag caacgacctc cggaagctct tcctcgattt cttcatcgag cgcgggcatc
     1441 cgcacctgcc gcaggccccg ctcgtcccgc tcgacgaccc gacgctcctc ttcacgtcgg
     1501 cggggatggt gccgttcaag ccctacttct cctcgccgct tccgcctccg acgcgacgcg
     1561 cggtgacggt gcagcgctgc ctcaggctca ccgacgtgga gcaggtggga cagacggtgc
     1621 gccacgcgac cttcttcgag atgctcggca acttctcctt cggcgactac ttcaagaagc
     1681 aggcggtcga gtgggcatgg gagttctcga cccaggtgct cgccttgtca ccggataggc
     1741 tctggccgtc ggtctacaag gacgacgacg aggcgttcga tctctgggcg aagcacatcg
     1801 ggctgggcgc cgatcgcgtg acgcggctcg gtgagaagga caacttctgg ggaccggccg
     1861 gcggcaccgg cgcgtgcggg ccgagctccg agatctactg ggatcagggc cccggcacgg
     1921 ggtgccagcg cccggagtgc gcgcccggct gcgactgcga gcgctacctc gagttctgga
     1981 acctcgtctt cccgcagttc gatcagctcc agagcggcga gcggaagccg ctcgccaatc
     2041 gcgggatcga cacgggcatg ggactcgagc ggctcgcgat gatcctccag gcctcgccgt
     2101 ccgtgcagga caacgacctc ttagcgccga tcggcgacgc cgtgcgccgc atgggaagcg
     2161 ctgccgacgc ggcgaccgat cgcgggcggc gcgcggtgcg cgtcatcacc gaccacgcgc
     2221 gcgcgctcgt cttcacattc gccgagggcg tgcggccgtc gaacgaagga cgcggctacg
     2281 tcgtccgccg gctcctccgg cgcgcggcga ggttcggccg cgacgtcggg atccagggac
     2341 ccttcctcgc gcggctcgtg cccacggtcg tccagcagat ggcggcgtac gacgagtacg
     2401 cgtacctcaa gcgcgaggaa tcgatcgtgg cggacgcgat gcgcgaggag gagtcgcgct
     2461 tcgcggagac gctcgagcag ggcatggccc gcttcgagga gaccgcctcg cagctcgaga
     2521 agcggcgcga gaagatcttc ccgggggcgg tcgcgttcca gctctacgac acgttcgggt
     2581 tcccgctcga tctcacggtg gagatggcga tggagcgagg gctcaccgtg gatcagccgg
     2641 gctacgaaac cgccatgcag gagcagcgcg atcgcgcgcg gaaagcgggc cgcttcgaga
     2701 cgcgccgcgc ggacgcggga ccgtggcagg ccctcaccca gggcgcgcac tccgcgttca
     2761 caggttacga tcagtgggcg ctcgacggcg tgtcgatccg atcgctccgc gagacgggaa
     2821 ccgaccgcga ggtggagctg acgctcgaca aaacaccctt ctatcccgaa gggggcgggc
     2881 aggtcggcga caccggctgg ctccagggcg agggggcgcg cctccgcgtg atcgatacct
     2941 accgggaggg cgatgcgatc ctgcaccgcg cggagatcgt ggagggcgcg gcccacgcgg
     3001 ggccctggcg tgccgtggtc gacgcggaga agcgcgcggc gacgcagcgg aatcacaccg
     3061 cgacccatct cctccatgcc gcgcttcgcg cgcgcctcgg cacgcacttg aagcaggccg
     3121 gctcgctcgt cgcgccggat cggctccgct tcgacttctc gcacggcaag gcgctcacgc
     3181 ccgaggacgt ccacgccatc gaatc
//
LOCUS       DASTKC010000262        20554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_465581, whole genome shotgun sequence.
ACCESSION   DASTKC010000262 DASTKC010000000
VERSION     DASTKC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 20554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20554
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465581"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="VFR25_10940"
     CDS             complement(<1..1779)
                     /locus_tag="VFR25_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941603.1"
                     /translation="MPEAIVWLVPALVAAALCASRLGLGYFWDDYIFLTMRGTGDWAA
                     NLRLDPSVFYYRPIPQGLYFLFLRFVDPRSGLVGHVVNLLLLMLCAALVTRWARKVAG
                     PWTGFLAGLAFATLGAAPSLVTWVTAAADLFAVSFLLLALNARHDGRLGLSALFAALA
                     LLSKESAAGWFPLLIGYDFVLGREPKRLARSAIAFGAVLVLWAGIHPGLHKLLGHGLR
                     APEGGYVGLGHFDRWGLYLGRYLLALVNVPATWTKTSWPVDLNPQVTLAAAVALVGFA
                     WVSRGTTSSGTGDAPSTARLVAMALLLSLPALALPILFVEIWVPYYIVIIAPGTCLLL
                     ALGLRMLPRVAAGLALAAFLLLGVWFRGMAFPTERVWSEPVLVDAAQAIDLVRLRALT
                     IQPTVPRRAQLLVSVAATGSRGIATTLFDGQAPSVWYRDPSVHAAKVEDRAAGYPADL
                     LWRITGDLHVIDIDPVEGRFRSTAPDVEAESVGRPIATYARAVAASGEPDRAIRILEH
                     LGEIDHEELRSYDLRLAASIALAYGQPAEAERLRSLAPAIDYETAVNMMGRVFGNPSR
                     NPGQDSCAYWAFQISPDDTVAIRHYLE"
     gene            1853..2278
                     /locus_tag="VFR25_10945"
     CDS             1853..2278
                     /locus_tag="VFR25_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9941604.1"
                     /translation="MNRLAGFAHSLLRIVSGFLFIHPGGMKLLGWFGGMPPGVELTPL
                     LVTAGVIELVCGTLIFLGLLTRPAAFLASGEMAFAYFIGHFPHGFWPIQNHGEPAVLF
                     CFIFLYFAASGAGPISVDHLLGLDRRRKPLGEEKPAAAS"
     gene            2435..4918
                     /locus_tag="VFR25_10950"
     CDS             2435..4918
                     /locus_tag="VFR25_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941605.1"
                     /translation="MIPPEGSEPPGPADDRLPEDPIDQIVASVCDGRPVDLSDEEFDA
                     LATRLGDRARALLDVAKIAAFSRALQREDQEARPRSQAPDTLGRWGPFTLLERLGSGT
                     SGEVWRAWDGSLEREVAVKFLLPRSDDESRKTLLAEARALARVRHPGVATVFGIAEHD
                     GRLGLWMELLRGPTLAEVIERKAPLSVRDVALLARKLCEALDALDHAGVLHRDIKPSN
                     IILETGGRAVLTDFGLGSRRRLFAAISPRDGSGTPLFMAPSLLEGGPATPRTDLYALG
                     VTLRWALTGRPPFEARTMDDLKAEARRGPSTPLASERADAPSELVRAIERAMDTSTEA
                     LPYTAASMKRDLGAAAAQPDETAATVVRDRPSVAVLPFLNRSQDPAEDYFSDGLAEEL
                     LTVLSKIGGLHVAARTSAFQFKGKNEDLATIGRKLRVGTIVEGSVRRSGNRMRVAVRL
                     VQADDGVQLWSESYDRVLEDLFLVQDDIARSVARELHATLTAALPTDALRRNVEDAVK
                     GRSQDPEAHRLYLEGRYLLLRRNRKDLALALERLRSAIARDPRSATVWAELGHATAEE
                     ADHGMAPADEAYARAREMIQRALDLEPDLSVAHARLARIQVVRDWDWPAAEASFLRAL
                     DRDPANVSAMHGAAVLLQALGRTDEALTLNRRAIDLDPLNMPSLHNLGCVYYEAGRLE
                     EAEAALRNVIAIQPVRQITHSVLAQVLEEMGRSEEALEEALREPDPAYRHLALALIHH
                     RAGRIAERDLVLHEIAETCGESEPVQVAEAYGACEMADDALAWLERASSTRDAAIVHI
                     KPSRHFRSIRRDPRFNSILKTIGMDATGT"
     gene            4915..7176
                     /locus_tag="VFR25_10955"
     CDS             4915..7176
                     /locus_tag="VFR25_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941606.1"
                     /translation="MIGTTLSHYRILEPLGAGGMGEVYLARDEHLDRDVAVKVIQRGM
                     LADPAARERFRREARVLARLSHPGVATIYDFDTQGSLDFLVMEYVPGGTISSRLKSGA
                     FPVPDVLRLGASIADALEEAHKRGFLHRDLKPGNVVLTTEGAPKLLDFGLAGLLPTGG
                     GQATELTQTGTILGSLPYMAPEQLRGDPADLRSDVWSLGALLYEMATGQRAFGKDRAE
                     ALMFEILHTMPRPLRSVRPEAPPALERVVENCLDKDRARRPGSAGDVARMLRAVLSGA
                     SAGSGKHDDPPTEISRDSIRSLAVLPFDNVSRDLAQEWFADGMTEALIADLARLQGLR
                     VISRTSAMKYKGMARSLPEIGHELGVDAILEGSALLVGPRVRITVRLVSARTDETLWS
                     ERYDDKLEDVLDVQSRVAESIAKEIELHLSPREAKRLAKRQAFKPEAHLEYLKGLHTI
                     AGSSPQAVELSLRHFRRALEIDPTYAPAWAGIADCHTIRASRGMAPPAEAIHEAEQAA
                     LKALTLDDSVADAHAVLGSVRLHQLDLASAIREEKRAVDLNPGLADAYQTLGRVLYCT
                     EQHTEAQAAMLEALRIDPMSMIIHTSVGDAYYYAREYERSIVYYRKATELDPRFDGAH
                     TDLARSLEALGRFDESKREYDEGRALSGGVAGPSFGLAHLAASRGDTAEARRMLAELT
                     EARSKRVVSAWGIAALHASLGDLDDAFAWLETAVKEQATGLIFLRVHPRLDPIRKDPR
                     YGELVRRLGLNVA"
     gene            complement(7199..8704)
                     /locus_tag="VFR25_10960"
     CDS             complement(7199..8704)
                     /locus_tag="VFR25_10960"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET9941607.1"
                     /translation="MESLQALKQRLTEIDDLRRATAVLSWDQTTYMPPAGGTARGRQM
                     ATLSRLAHESFIDAETGRLLDAVARETESLPHDSDEASLVRVTRRRWDRDRRMPASLV
                     SEIEENAALSYQAWTAARPANDFERMRPYLERTLELSRRAADCHPGYDHIADPLIDAH
                     DHGMKTADVRRLFHSLRTRLEPLVQAIASQPQLDDAPLRKTIPLETQLRFGLEVIRAF
                     GFDFARGRQDMTRHPFMTRFAHGDVRITTRSRDNDVTDSLFSTLHECGHALYEMGFDP
                     SFEGTPLAGGSSSGVHESQSRLWENLVGRSRGFWEHFLPRMQAIFGRDLDTVSVDAFY
                     RAINRVERSLIRTDADEATYNLHVMLRFDLELDLLEGKLAVRDLARAWRERFEASMGV
                     AVPDDANGVLQDVHWYHGLIGGQFQGYTLGNILSAQFFATAVKARPEIPADIRRGSFG
                     GLHDWLKENIYRHGSKFTPSELTKRVTGSDLSIEPYMAYLEGKFRPLYGLS"
     gene            complement(8836..9822)
                     /locus_tag="VFR25_10965"
     CDS             complement(8836..9822)
                     /locus_tag="VFR25_10965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9941608.1"
                     /translation="MVPKRSLGQGLQVSALGLGCMGMSEFYGARDDEESIATIHRGLD
                     LGVTFIDTADVYGPYTNEELVGRAIRGRRNEVVLATKFGNVRDERGAWVGQNSRPDYV
                     RQACDASLRRLGVDHIDLYYQHRVDRSVPIEDTVGAMAELKQAGKIRYLGLSEAAPDT
                     IRRAHRVHPISALQTEYSLWTRDPEAGVLAACRELGIGFVPYSPLGRGFLSGRFRSES
                     DLAESDARRNHPRFQGENFRKNLDLVRRVEEIAKEKRCTPAQLALAWLLSRGSDIVPI
                     PGTKRREYLEENVKSLEIRITPEDSHRIDEVAPVGATAGTRYPEGGMLTING"
     gene            complement(9855..10706)
                     /locus_tag="VFR25_10970"
     CDS             complement(9855..10706)
                     /locus_tag="VFR25_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9941609.1"
                     /translation="MSHDPVRPPIDPEVREYYARSPEETRLDRGPFQLERVRTEELIR
                     RHAPPPPARVLDVGGGAGAYSFDLADRGYEVHLLDASERLIDHARQRGGGRLASLQVG
                     DARALPVPDVFADTVLLLGPLYHLTSAQDRVTALREAGRALRPGGWLFAAAISRFASS
                     MDGLFRDLFQDPRFQAIAERDLAEGQHRNTTEREDYFTTSYFHRPEDLAAEVLAAGLD
                     LVEVYGVEGPGWTLADFEARWSDPRARGDLIRVARQLEAEPSIRGASAHLMAVARRPS
                     IDHHAAR"
     gene            10698..11609
                     /locus_tag="VFR25_10975"
     CDS             10698..11609
                     /locus_tag="VFR25_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9941610.1"
                     /translation="MTHGGFIIDQTRIGGAALRVADLERSLDYYRNVLGFVPLDGGTS
                     ARVARLGAAGSATPLLELREHRGARPVPRRGRIGLYHVAVLLPSRGDLGRFLDHVMTR
                     DERLGASDHGVSEALYLTDPDGITIEVYRDRPRSEWPTQGGHISMGNEPLDGDAVLRA
                     GGGAPFEGLPEGTTLGHVHFYVEDLEKARAFYVDALGFDVTFEGYPGALFVASGGYHH
                     HVGLNTWALGSPIAGPDDAGIVHWELVLPDARTVEATAARARAAGFDVGADDAVVQAR
                     DPWGITVHLLDGRAVHTAGAPSREGGR"
     gene            11606..12208
                     /locus_tag="VFR25_10980"
     CDS             11606..12208
                     /locus_tag="VFR25_10980"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9941611.1"
                     /translation="MTEAAPADDRPPAPELMITGDLLSRAKGGDRAALGALMERYRPR
                     LVRWASGRLPAYARSLLDTNDLVQETLLRAIEVLDSIEVRGPGVFQAYVRQAVLNRIR
                     DQVRWARRRPGQDGVPETLEDAAPSPLERAIGTETLDRYERALARLTEDDRRLLHLRI
                     ELDFSYEEVAAITERGSRDAARMAVQRALRKLADVMGHER"
     gene            12198..12443
                     /locus_tag="VFR25_10985"
     CDS             12198..12443
                     /locus_tag="VFR25_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941612.1"
                     /translation="MSADDKLDSLAGSVSDGRPVDWVHEETEANVDEESGVRALRDVS
                     LIAEFSRDLQRGVIDEDATSSDERAEDVASEVPDDDK"
     gene            complement(12496..13314)
                     /locus_tag="VFR25_10990"
     CDS             complement(12496..13314)
                     /locus_tag="VFR25_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941613.1"
                     /translation="MAVVPGTRIYYIVDDPKYDLSGSNETVYLVEDGSSYRAGAKGVP
                     VALVKTSTGAHEVVAIPAEYRQNWMAVAAGDRPVRTVVPSRMVSTAVPTMIVVSSDRV
                     YGTNGQLIYSDQRVRDNGRYRTTSASHASSTQRATYHRHHKASSAKAASVKKRHHRST
                     SATNASYARKNAYRNGYNAGYRTAAAEHRVPAENTGVSAMKTAAARYAEEVRSQPDAF
                     QFQNSWYRREAGGWTRSYSWRGPFVYVDKGSVPREVKESEMRGSKLGSTEVIGD"
     gene            13547..14455
                     /locus_tag="VFR25_10995"
     CDS             13547..14455
                     /locus_tag="VFR25_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9941614.1"
                     /translation="MNALLRNLRRTLRRFQDHEGPRVAAAIAFYVLFSSVPALAFLIA
                     SVTWVIRDPHDQAHIVSHLLELLPTGSEQNRAMLMGIVNSVRKASRGITVLGALGLAW
                     SSLGMFSAARWGLNRAWGTKSRANFFGVRLMDLAAAVGIWMLLLLSALATAAVHLLAG
                     EGAPLPATSWLPGLASTAIQSSIPAALSFAAFLFVYRYVPSVEHRMRDVFPAALVATC
                     AFELTKHLFAFYVRLMTKSSPLFAALGGTLGFMLWVYLCAAILLLGAELGYVSSRYAL
                     SHEVLLDQPKGKDRRRQHSRESKQKA"
     gene            complement(14626..15084)
                     /locus_tag="VFR25_11000"
     CDS             complement(14626..15084)
                     /locus_tag="VFR25_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9941615.1"
                     /translation="MKRITVLTLVLLALALQSGRAAGADKRDLVSEVRDSERAFAKTM
                     ADRNLNAFGGYVAEEGVFFGGKGPLRGRTAVVAAWTRFFEGKEAPFSWEPETVEVLPS
                     GNLALTSGPVHDPKGTLIGTFSTIWRLEPDGRWRVVFDKGCEVCAPEPKH"
     gene            15173..17047
                     /gene="nagB"
                     /locus_tag="VFR25_11005"
     CDS             15173..17047
                     /gene="nagB"
                     /locus_tag="VFR25_11005"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF002557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET9941616.1"
                     /translation="MTYTAVMRDNPERIPVVIAESYDKLALRVAQRIAALIRERHKVG
                     LPTVLGLATGSTPIGIYRELIRMHRSEGLDWSRVVTFNLDEYYPMAPDSFHSYHRFMW
                     ENLFGQVNIDRKNVHIPRGDLPRDQVDAHCAEYESQIREAGGIDFQILGIGQTGHIGF
                     NEPGSSAASRTRLVTLDTITRRVAASDFFGTENVPVEAVTMGVATILEAREIALLATG
                     EHKAAIVKRAVEGDVDRSVAATYLQGHPAATVFLDSAAAAELTRVKTPWLLGEIAWTE
                     ALEIQAVVWLAGETGKSILKLDDIDYREHRLTPLLARHGSSGAINGLVFNALSAKVRG
                     RSKLPVGKRVIVFSPHPDDDVISAGGTLRKLHQNGNHLVVAYQTSGNIAVFDHELRRY
                     LDFVCRASQDFGIASDRLPRFVAEVDAFLKARKPGEVDSGEVLLLKRRIREVEARSGL
                     ETLGIGADASRFLNLPFYQTGQVRKDPIGDRDVAIIQELLTAERPDLILVAGDLSDPH
                     GTHRMCYEAVARALERYDGKPPEVWLYRGAWQEWPIDEADLLVPMSEEELRLKISAIF
                     KHQSQKDRAPYPGVDEREFWQRVEERNKGTAALVDRLGLPEYYAMEALVVRSARLLPE
                     "
     gene            complement(17050..20037)
                     /locus_tag="VFR25_11010"
     CDS             complement(17050..20037)
                     /locus_tag="VFR25_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9941617.1"
                     /translation="MSPSEVDPIHDVSNPFQAFQDLMPFRVQDILLVSSLYDSFTLQE
                     DGRLNELIAGEFLELSLHHTPGLTHVSSGHEALALARSERRFNLILSTLRPADMDATE
                     IAREVKRAGLDIPVVGMAYDNDERKEFEARHDVSELDGLFLWQGNARILVAIVKYVED
                     RRNVFHDTAAMGVRVLLVVEDNVRYYSSFLPEIYTELIRQSERLISEGINPSHKLVRT
                     RARPKLLLASTFEQAWELVQKYEPYLLGLITDVEFPRGGKRDRNAGFDLAKLVRSRIP
                     DLPIVLQSSRAEFAAGAAQVGAAFLRKYSETLLSDLRAFLVENFAFGEFVFRLPDGTE
                     VARARDLKSLEEKLKTVPAESIGYHAQRNHFSNWLGARTEFALARKLRPRRVEDFGSL
                     EGLRHNLIESIAEYRREQTEALVADFDPATFDPDSNFFARIGNGSLGGKARGLAFVRY
                     LINYLGIGRGIPGVRIAVPQTVVLATDCFDRFLERNELLDLALNSRDDEKILAEFLRA
                     PLPRDVMRKLRAFVQSAHWPLAVRSSSLLEDSQYQPFTGVYETFMLPNNHPDPRVRLR
                     QLVGAVRRVYASTFLSQTKDFLHNTPYRLEEEKMAVAIQRVVGAHHGTRFYPDFSGVA
                     RSYNYYAAPPLQTEDGIAAVALGMGRSVVEGERCLTFCPRHPQAILQFSTPDEIVRNS
                     QRDFWAVELNGGDTDGEQGMREKRFGLEAAEADGTLYALGSTWSAENQAVYDGLARPG
                     VRLVTFAPVLKHGVFPLAEILSILLDAGSRGMNRPAEIEFATVLSKDPSKPHHFGFLQ
                     MRPLVLAHETENLELEEPPKDALLCRSTQVMGNGLVDDIRDAVVVDFHRFDRARSRGA
                     ASAVAQFNAELLQAKRPYLLIGVGRWGSTDPWLGIPVTWDQISGARVIVESGFRDVRV
                     TPSQGSHFFQNLTSFGVGYFTVNEDLGEGFIDWDWIEGQPQLGEAEAVRHVRFERPLI
                     VKMNGRKNLGLIYKPGADAGRPA"
     gene            complement(20199..>20554)
                     /locus_tag="VFR25_11015"
     CDS             complement(20199..>20554)
                     /locus_tag="VFR25_11015"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HET9941618.1"
                     /translation="ASNLVKNSVMLVAEGANMPSTIEAIQVYLDAGILYGPAKAANAG
                     GVATSGLEMSQNSMRLAWSRDEVDHRLHQIMVAIHQTCFETAEKYGTPGNLLNGANIG
                     GFLKVANAMLDQGLV"
BASE COUNT         3338 a         6991 c         6991 g         3234 t
ORIGIN      
        1 ttcgaggtag tgccggatgg ccaccgtgtc gtccggcgag atctgaaacg cccagtaggc
       61 gcacgaatcc tgccccgggt tcctggatgg attcccgaag acgcgcccca tcatgttgac
      121 ggcggtctcg tagtcgatcg caggcgccaa ggagcgcagg cgctccgcct cggccggttg
      181 gccgtaggcg agggcgatcg atgccgcgag gcggagatcg taggaccgga gctcctcatg
      241 gtcgatctcg cccagatgct ccaggatgcg aatcgcccga tccggctcgc ccgacgccgc
      301 cacggcgcgc gcgtacgtgg cgatgggccg gcccacgctc tcggcctcga catccggcgc
      361 ggtcgaacgg aaacggccct cgaccggatc gatgtcgatc acgtgcagat ctcccgtgat
      421 ccgccagaga agatctgctg ggtaaccggc cgcgcgatcc tcgaccttgg cggcatgcac
      481 ggatgggtcg cgataccaga cgctgggcgc ctgcccgtcg aagagcgtcg ttgcgattcc
      541 tcgcgagccg gtcgccgcca ccgaaacgag cagctgagcc cttcgcggaa cggtgggttg
      601 gatcgtcaga gcgcggaggc gcacgaggtc gatggcttgt gcggcatcga ccagcaccgg
      661 ctcggaccag acccgctcgg tgggaaacgc catcccgcga aaccagacgc cgaggagcag
      721 gaaggccgcg agtgcgagcc ccgcggccac gcgaggcagc atccgaaggc cgagcgcgag
      781 caggaggcag gtccccggcg cgatgatcac gatgtagtac ggcacccata tctccacgaa
      841 gaggatgggg agggcgagcg cggggagcga gagtagaagc gccatcgcga cgagacgagc
      901 ggtcgatggc gcgtcccccg tgccgctcga ggtcgttcca cgactgaccc aggcgaaacc
      961 gacaagagcg accgcggccg cgagcgtgac ttggggattg agatccacgg gccaggaggt
     1021 cttggtccat gtcgcgggca cgttgacgag cgcgaggaga tagcgtccga gatagaggcc
     1081 ccatcggtcg aagtgtccca ggcccacgta cccgccttcg ggcgcccgaa ggccgtggcc
     1141 gaggagcttg tggagcccgg gatgaatccc cgcccacagc acgagcacgg cgccgaacgc
     1201 gatcgcggac cgcgcgaggc gcttcggctc gcggccgagc acgaagtcgt atccgatcaa
     1261 gaggggaaac cagccggcag ccgattcctt ggagaggagt gcgagagcgg cgaacagggc
     1321 cgaaagaccg agacggccgt cgtgacgggc gttgagcgcc agaagcagga acgagaccgc
     1381 gaagagatcc gccgcggcgg tcacccaggt cacgagactc ggcgccgctc cgagcgtcgc
     1441 gaacgccagc ccggcgagga accccgtcca cggacccgcg accttccgcg cccatcgcgt
     1501 gacgagcgcc gcgcacagca tgagaaggag caggttgacg acatgcccca ccaggccgga
     1561 tcgcgggtcg acgaaacgaa ggaagagaaa gtagagccct tgcggaatcg gtcggtagta
     1621 gaacacggac ggatcgagcc gtagattcgc cgcccaatcg ccggtgccgc gcatcgtgag
     1681 gaagatgtaa tcgtcccaga agtatcccag gccgagacgc gatgcgcaga gcgctgcggc
     1741 cacgagagcg ggcacgagcc acacgatggc ctcgggcacg ctccgcgcgg aagagcgtgc
     1801 taccctagtt gggttggcgg accgtgatcg tcgcttgtgg gaggctcgag tcatgaaccg
     1861 cttggccggc ttcgctcatt cgctcctgcg catcgtgtcg ggattcctct tcatccatcc
     1921 gggcgggatg aagctcctcg gctggttcgg cggcatgccg cccggcgtcg agttgacgcc
     1981 cctcctcgtg accgcgggcg tgatcgagct cgtctgcggc acgctcatct tcctcggcct
     2041 cctgacccgc ccggcggctt tcctcgcgtc gggggagatg gcgttcgcgt acttcatcgg
     2101 ccatttcccg cacggcttct ggcccatcca gaaccacggc gagcccgcgg tgctcttctg
     2161 cttcatcttc ctctactttg cggcgagcgg agcgggaccc atcagcgtcg atcacctcct
     2221 cgggctcgat cgacgacgca agccgctggg cgaggagaaa cccgcggcgg catcgtagga
     2281 ccctgggcga tcgcgctcca gaccaattcg acgacctcgg gtggaggctc ggcgaggctt
     2341 gccgggaccc tctttcatat ggcatgattt caagctctta ccgcatttgg atcgcgcaac
     2401 gcttcagacg cgagaggctg ccccggacag caagatgatc ccacccgaag ggtctgagcc
     2461 tcccggcccg gcggacgacc gcctgcccga ggacccgatc gaccagatcg ttgcctccgt
     2521 gtgcgacggc aggccggtcg acttgagcga cgaggaattc gacgcgctgg cgacccggct
     2581 cggagaccgc gcgcgcgcgc ttctcgacgt cgccaagatc gccgcgttca gccgcgccct
     2641 ccagcgcgag gatcaggagg cgcgccccag aagccaggcg cccgacacgc tcgggcgatg
     2701 gggcccgttc acgcttctcg agcggttggg ctcgggcacg tcgggtgaag tgtggcgcgc
     2761 ctgggacgga tcgctggagc gcgaggtcgc ggtcaagttc ctgctgcctc gctcggacga
     2821 cgagtcgcgg aagacgctcc tcgccgaagc acgcgcgctg gcccgcgttc gccacccggg
     2881 cgtggccacg gtgttcggaa tcgccgagca cgacggccgc ttgggcctgt ggatggagct
     2941 tcttcgtggc cccacgctcg cggaggtcat cgagcgaaaa gcgccgctca gcgttcggga
     3001 cgtggcgctc ctggcgcgca agctctgcga ggcgctcgac gcgctcgacc acgcgggcgt
     3061 cctgcaccgc gacatcaagc cctccaacat cattctcgag acgggaggac gggccgtcct
     3121 caccgacttc gggctcggct cgcgccgccg cctcttcgcc gccatctccc ctcgcgacgg
     3181 gtcggggacg ccgctcttca tggcgccctc gctcctcgag ggcgggccgg ccacgccgcg
     3241 caccgacctc tacgcgctcg gggtgacgct tcgatgggcc ctgacaggac gccctccctt
     3301 cgaggcgcga acgatggacg atctcaaggc ggaggcccgc cgcggtccgt ccacgccgct
     3361 cgcgagcgag cgggccgacg cgccctccga actcgtgcgc gcgatcgaac gcgccatgga
     3421 cacttcgaca gaggcgctcc cctacacggc cgcctcgatg aagcgggacc tcggcgctgc
     3481 ggccgcacag cctgacgaaa ccgcggcgac agtcgtccga gaccgtccgt ccgtcgccgt
     3541 tcttccattc ctcaaccgga gtcaggatcc cgcggaggac tacttctccg acggcctcgc
     3601 ggaggagctg ctcacggtcc tctccaagat cggcggcctc cacgtggccg cgcggacctc
     3661 cgcgttccag ttcaagggaa agaacgaaga cctcgccacc atcgggcgga agctcagggt
     3721 cgggacgatc gtcgaaggca gcgtccggcg gtcagggaat cggatgcgcg tggccgtgcg
     3781 gctcgtccag gcggacgacg gcgtgcagct ctggtcggag tcgtacgacc gcgtgctcga
     3841 ggatctcttc ctggtgcagg acgacatcgc gcgcagtgtc gcgcgggagc tccacgcgac
     3901 gctcacggca gcgctcccca cggacgcgct tcgccggaac gtcgaggacg cggtcaaggg
     3961 acggagccag gaccccgagg cccaccgact ctatctcgag ggacggtatc tcctcctgcg
     4021 tcgcaaccgc aaggacctcg cactcgcgct cgaacggctg cgctcggcga tcgcgcggga
     4081 tccgagaagc gctactgtat gggccgagct cggccatgcc acggcggagg aagcggacca
     4141 cggcatggcc cccgccgacg aagcctatgc tcgggcgcgg gaaatgatcc agcgtgcgct
     4201 cgacctcgag cccgatctct ccgtggcgca cgcgcggctc gcgcgcatcc aggttgtccg
     4261 cgactgggat tggcccgccg cggaggcctc gttcctgcgc gccctcgacc gcgatccggc
     4321 gaacgtctcg gcgatgcacg gggccgcagt cctcctccag gccctcgggc gtacggacga
     4381 ggcgctgacg ctcaatcgac gggccatcga cctggatccc ttgaacatgc cgtcgctcca
     4441 caacctcggc tgcgtctatt atgaggcggg gcggctcgag gaagcggagg ctgcgctccg
     4501 gaacgtcatc gccatccagc cagtcaggca gatcacacac tcggtgctcg cacaggtgct
     4561 cgaggaaatg ggacgaagcg aagaagcgct cgaagaagct ttgcgcgagc ccgacccggc
     4621 gtaccgacat ctcgcgctcg ccctcatcca ccatcgcgca ggacgcatcg ccgagcgcga
     4681 cctggttctg catgagatcg cggagacctg cggggagagc gaaccggtcc aggtcgccga
     4741 ggcgtatggc gcctgcgaaa tggccgacga tgcgctcgca tggctcgagc gtgcctcctc
     4801 gacgcgcgac gcggcgatcg tccacatcaa gccgagccgc catttccgtt cgatccgaag
     4861 ggacccgcgt ttcaactcga ttctcaagac catcggcatg gacgccaccg gaacgtgatc
     4921 ggaaccacgc tctctcatta ccgaatcctc gagccgctcg gtgccggtgg catgggcgag
     4981 gtgtacctcg cgcgcgacga gcacctggac cgcgacgtcg ccgtgaaggt catccagcgc
     5041 ggcatgctcg ccgaccccgc cgcgcgcgag cgtttccgcc gcgaggcccg cgtgctcgct
     5101 cggctctccc atccgggcgt cgccacgatc tacgacttcg acacgcaggg gagcctcgac
     5161 ttcctcgtga tggagtacgt ccctggcggc acgatctcgt cgagactcaa gtccggggcc
     5221 ttcccggtcc ctgacgtgct tcgcctggga gcctcgatcg ccgacgcact ggaggaagcc
     5281 cacaagcgcg gcttcctcca ccgcgacctc aaaccgggca atgtggtgct cacgacggaa
     5341 ggagcgccga agcttctcga cttcgggctc gcgggactcc tccccacggg cgggggacag
     5401 gccacagagc tcacgcagac ggggaccatt ctcggttccc ttccctacat ggcccccgag
     5461 cagctccgcg gagatcctgc ggatctccgg agcgacgtgt ggtctctggg tgcactcctc
     5521 tacgagatgg cgactggaca gcgcgcgttc ggaaaggacc gggcggaggc gctcatgttc
     5581 gagatcctcc acaccatgcc ccgcccgctc cgcagtgtcc ggccggaagc tcctccggcg
     5641 ctcgagcggg tggtcgagaa ctgcctcgac aaggatcgcg cgcgccgacc gggctcggcc
     5701 ggcgatgtcg cgcgcatgct gcgcgcggtc ttgtccgggg cttccgcggg atccggaaag
     5761 cacgacgacc cgccgaccga gatctctcga gactcgatcc gctcgctcgc ggtgctcccc
     5821 ttcgacaacg tatcccgcga tctggcgcag gagtggttcg cagacggcat gaccgaagcc
     5881 ctgatcgctg atctcgcacg gctgcagggt ctgcgtgtca tctcgcgcac gtcggctatg
     5941 aagtacaaag ggatggcacg ctccctgccg gagatcggcc acgagctggg cgtggatgcc
     6001 atcctcgagg ggtccgccct cctggtcggg ccgcgcgtgc ggatcacggt gcgcctcgtg
     6061 tcggcgcgca ccgatgagac gctctggtcg gagcgctacg acgacaagct cgaggacgtg
     6121 ctggacgtgc agagcagggt ggccgaatcg atcgcgaagg agatcgagct ccacctgtcc
     6181 ccgcgcgagg cgaaacgact cgcgaagcgg caggcattca aaccggaagc ccacctggag
     6241 tacctgaagg ggctccacac gatcgccggc tcatcgcccc aggcggtgga gctcagtctc
     6301 cgccacttcc gccgggcgct ggagatcgac ccgacctacg cgcccgcgtg ggccgggatc
     6361 gcggactgtc acaccatccg cgcctcgcgc ggcatggcgc ctcccgccga ggcgatccac
     6421 gaagcggagc aggcggccct caaggcgctg acgctcgacg attccgtggc cgacgcgcac
     6481 gccgtccttg gatccgtccg tctccatcag ctcgacctcg cgagcgccat ccgcgaggag
     6541 aagcgcgccg tcgacctcaa tccaggactg gcggatgcct atcaaaccct ggggcgcgtc
     6601 ctctactgca ccgagcagca caccgaagcc caggcggcca tgctcgaggc gctccggatc
     6661 gaccccatgt cgatgatcat ccacacatcc gtcggcgacg cctactacta cgcgcgagag
     6721 tacgagcgct cgatcgtgta ctaccggaag gcgaccgagc tcgatcctcg attcgacggg
     6781 gcgcacaccg acctcgcgcg ctcgctcgag gcgctcggcc gcttcgacga atcgaagcgg
     6841 gaatacgacg agggacgagc cttgagcgga ggcgtcgccg gtccgtcgtt cggcctcgcg
     6901 cacctggcgg cgagccgcgg ggacacggcg gaagcccgcc ggatgctcgc ggagctcacg
     6961 gaggcgcggt cgaagcgggt cgtgtcggcg tggggcatcg ccgcgctcca cgcgtcactc
     7021 ggcgacctgg acgacgcctt tgcatggctc gagacggccg tgaaggagca ggcgacgggg
     7081 ctcatcttcc ttcgggtgca cccgcggctc gatccgatcc gcaaggatcc gcgctacggc
     7141 gagctggtga ggcgactcgg actcaacgtc gcctgacgcc cggcctcggc gcgccgcgct
     7201 acgatagccc gtagagcggc cggaacttcc cttccaggta cgccatgtag ggctcgatcg
     7261 agaggtcgct ccccgtcacc cgcttcgtga gctccgacgg cgtgaacttg gagccgtggc
     7321 ggtagatgtt ctccttgagc cagtcgtgga ggcctccgaa cgagccccgc cggatgtcgg
     7381 ccgggatctc ggggcgcgcc ttcacggcgg tcgcgaagaa ctgagcggag aggatgttcc
     7441 ccagggtgta tccctggaac tggcccccga tcaacccgtg ataccagtgg acgtcctgga
     7501 gcacgccgtt cgcatcgtcg ggcaccgcca cccccatcga ggcctcgaat cgctcgcgcc
     7561 aggctcgcgc gagatcccgc acggcgagct tcccctcgag gagatcgagc tccagatcga
     7621 accggagcat gacgtggagg ttgtaggtgg cctcgtcggc gtcggtgcgg atgagcgatc
     7681 gctcgacacg gttgatggcg cggtagaacg cgtccacgct caccgtgtcg agatcgcgac
     7741 cgaagatcgc ctgcatccgg ggaaggaagt gctcccagaa gccgcggctc cgccccacga
     7801 gattttccca gaggcgggac tgactctcgt gcacgcccga ggaggatccg ccggcgagcg
     7861 gcgtcccctc gaacgaggga tcgaagccca tctcgtagag cgcgtggccg cactcgtgca
     7921 gcgtggagaa gagcgagtcg gtgacatcgt tgtcccggga ccgcgtcgtg atgcgcacgt
     7981 cgccgtgcgc gaaccgcgtc atgaagggat gccgcgtcat gtcctggcgg ccgcgcgcga
     8041 agtcgaaccc gaacgcgcgg atcacctcga gcccgaaccg aagctgcgtc tcgagcggga
     8101 tggtctttcg aagcggcgcg tcgtcgagct gaggttgtga ggcgatcgcc tggacgaggg
     8161 gctcgagcct cgtgcgaagc gagtggaaga gccgccgcac gtccgcagtc ttcatcccat
     8221 ggtcgtgtgc gtcgatcagg ggatccgcga tgtggtcgta gcccggatgg cagtcggccg
     8281 cgcggcgcga cagctcgagc gtccgctcga gatagggccg cattcgctcg aaatcgttgg
     8341 ccggcctggc tgcggtccag gcctggtagc tgagcgccgc gttctcctcg atctcggaga
     8401 cgagcgacgc cggcatccgg cggtcgcgat cccagcggcg gcgcgtcacc cgcaccagcg
     8461 acgcctcgtc ggaatcgtgg ggcaaggact ccgtctcacg ggccacggcg tcgagaagcc
     8521 gccccgtctc cgcgtcgatg aagctctcgt gcgcgagtct cgacagcgtg gccatctgcc
     8581 ggccgcgcgc ggtgccgcct gccggaggca tgtacgtcgt ctgatcccag ctcagcacgg
     8641 ccgtcgcgcg tctgaggtcg tcgatctcgg tgagcctttg cttcagagcc tggagcgatt
     8701 ccatgcggtc tccccggggg tccggtccat gcgatcgagg caagaccggg gagtataccg
     8761 aggcatgaac caccgtttga aggacgatcc gcccggcctc gtcgccgcca ggcccgccct
     8821 tccccagggg cacggttacc cgttgatcgt gagcatgccc ccttcgggat agcgggtccc
     8881 cgcggtcgcg cccaccggcg ccacctcgtc gatccggtgc gaatcctcgg gcgtgatccg
     8941 gatctcgagc gacttcacgt tctcctcgag atactcccgg cgcttcgtgc cgggaatggg
     9001 aacgatgtcg ctcccgcgcg agagaagcca cgccagggcg agctgggcgg gcgtgcaccg
     9061 cttctccttg gcgatctcct cgaccctccg aacgaggtcg agattcttgc ggaagttctc
     9121 accctggaac cgagggtggt tcctgcgcgc gtcgctctcg gccagatccg actcgctccg
     9181 gaagcggccc gataggaatc cgcggccgag aggactgtag ggaacgaagc cgattcccag
     9241 ctcccggcac gcggcgagca ctcccgcctc gggatcgcgg gtccagagcg agtactcggt
     9301 ctggagggcg gagattggat ggacgcgatg cgcgcgccgg atcgtgtcgg gcgcggcttc
     9361 ggagagaccg aggtagcgga tctttccagc ctgcttgagc tccgccatgg cgccgacggt
     9421 gtcttcgatg ggcacggagc gatcgacgcg gtgctggtaa tagaggtcga tgtggtcgac
     9481 gccgagacgg cggagcgagg cgtcgcaggc ctgccgcacg tagtcgggcc ggctattctg
     9541 cccgacccac gcccctctct cgtcccgcac gttcccgaac ttggtcgcca gcacgacctc
     9601 gttccggcgg ccccggatgg cgcgccccac gagctcctcg ttcgtgtagg gcccgtacac
     9661 gtccgccgta tcgatgaacg tgacgccgag atcgagcccg cggtggatcg tggcgatgga
     9721 ctcctcgtcg tcgcgtgcac cgtagaactc ggacattccc atgcagccga gccccagggc
     9781 cgagacctgg agcccctgcc ccagcgatcg ctttggaacc atgtcgctct cctttcctga
     9841 cgaacggcgc gcgatcagcg ggccgcgtgg tgatcgatgg aggggcgtcg cgcgaccgcc
     9901 atcaggtgtg cgctcgctcc gcggatcgag ggctccgcct cgagctgtcg agcgacgcgg
     9961 atcaggtccc cgcgggcgcg cggatcgctc cagcgcgcct cgaagtcggc cagcgtccag
    10021 cccgggccct cgacgccgta gacctcgacg agatcgagcc ccgctgccaa gacctcggcc
    10081 gccagatcct cgggcctgtg gaaatagctc gtcgtgaagt agtcctcccg ctccgtcgtg
    10141 ttgcgatgct gtccctccgc cagatcccgc tcggcgatcg cctgaaagcg cgggtcctgg
    10201 aagagatcgc ggaaaagccc gtccatcgag gaggcgaagc gcgagatcgc cgcggcgaag
    10261 agccagccgc ccggccggag cgcgcgccca gcttcgcgga gcgccgttac ccgatcctgg
    10321 gcggaggtca gatggtagag cggtccgagg aggaggacgg tgtcggcgaa cacgtccggc
    10381 acggggagcg cgcgcgcatc cccgacctgg agcgaggcga ggcggccccc acctcgttgc
    10441 ctcgcatggt cgatcagtcg ctccgaggcg tcgaggagat ggacctcgta gccccgatcc
    10501 gcgagatcga acgagtaggc tccggctccg ccgccgacgt cgagcacgcg cgccggcgga
    10561 ggcggcgcgt ggcgccggat cagctcctcc gtccggaccc gctcgagctg gaacgggcct
    10621 cggtcgaggc gtgtctcttc cgggctccgg gcgtagtatt cccggacctc gggatcgatt
    10681 ggcggacgca cgggatcatg actcatggag gcttcatcat agaccagact cgaatcggcg
    10741 gtgccgcgct ccgcgtcgcg gatctcgagc gatcgctcga ttactaccgg aacgtgctcg
    10801 gcttcgtgcc gctggacggt gggacctcgg cgcgcgtcgc gcggctcggc gcggcgggca
    10861 gcgcgacgcc gctcctcgag ctgcgggagc accggggcgc gcggcccgtt ccgcgccggg
    10921 ggcggatcgg cctctatcac gtcgccgtgc tccttccctc gcgcggggat ctgggtcgct
    10981 tcctcgatca cgtgatgacg cgcgacgagc gtctcggcgc gtccgatcac ggtgtgagcg
    11041 aggcgctcta cctcaccgat cccgacggaa tcacgatcga agtctatcga gaccgtccgc
    11101 gaagcgaatg gcccacccag ggagggcaca tctcgatggg aaacgagccg ctcgacggcg
    11161 acgcggtgct ccgtgcggga ggcggcgctc ccttcgaagg cctccccgag ggcacgaccc
    11221 tcggtcacgt gcacttctac gtcgaagatc tggagaaggc gcgtgcgttc tacgtggatg
    11281 ctctgggatt cgacgtgacg ttcgagggat accccggcgc ccttttcgtc gcttcgggcg
    11341 gctatcacca tcacgtcggc ctcaatacgt gggccttggg ctcgcccatc gcggggccgg
    11401 acgacgctgg gatcgtgcac tgggagctcg tgctgccgga tgcgcggacg gtggaagcca
    11461 cggccgcccg ggcgcgtgcg gcgggatttg acgtgggcgc ggacgacgcc gtcgtgcagg
    11521 cccgcgaccc atggggcatc acggtccacc ttctggacgg gcgggccgtc cacaccgctg
    11581 gggccccgtc ccgagagggc gggagatgac cgaggccgct cccgcggacg atcgcccgcc
    11641 tgctcccgag ctcatgatca cgggcgacct cctcagccgg gccaaggggg gcgaccgcgc
    11701 cgcgctcgga gccctgatgg agcgctatcg cccccgcctc gtgcgctggg ccagcggccg
    11761 gcttcccgcc tacgcccgtt ccctcctcga taccaatgat ctggtgcagg agacgctgct
    11821 tcgcgccatc gaggtcctgg attcgatcga ggtccgcggg ccgggggtgt tccaggccta
    11881 tgttcgccag gccgtcctca atcgtattcg agatcaggtg cgctgggcgc gaaggcggcc
    11941 cgggcaggac ggcgtccccg agaccctgga ggacgcggcc ccgtccccgc tcgagcgcgc
    12001 gatcggcacc gagaccctgg accggtacga gcgcgcgctc gcgcggttga cggaggacga
    12061 tcgccgcctg ctccatctgc gcatcgagct cgatttcagc tacgaggagg tggccgccat
    12121 caccgagcgg ggaagcagag acgcggcacg catggccgtc cagcgcgcgc ttcggaagct
    12181 ggcggacgtc atgggtcatg agcgctgacg acaagctgga ctccctcgcc ggatcggtct
    12241 ccgacggacg tcccgtcgat tgggtccacg aagagacgga ggcgaacgtc gacgaggagt
    12301 cgggcgtgcg cgcgctccgg gacgtatcgc tgatcgcgga attcagccgg gacctccagc
    12361 gcggcgtcat cgacgaggac gcgacctcga gcgacgagcg cgcggaggat gtggcaagcg
    12421 aagttcccga cgacgacaag tgaccgggac cccgaagggc cccggtcatc gttccccccg
    12481 cactaccgat cggcgtcagt cgccgatgac ttcggtgctc ccgagcttgc tgccgcgcat
    12541 ttcggattcc ttcacctcgc gcggaacgct ccccttgtcg acgtacacga agggaccgcg
    12601 ccaggaatag gagcgcgtcc acccgccggc ctcgcgccgg taccacgagt tctggaactg
    12661 gaaggcgtcg ggctggctgc gcacttcctc ggcatagcgg gccgcggccg tcttcatcgc
    12721 ggagacgccg gtattctcgg ccggcacgcg atgctcggcc gccgccgtcc ggtaacccgc
    12781 gttgtagcca tttcggtacg cgttcttccg cgcgtacgac gcgttcgtgg cggacgtgga
    12841 acggtggtga cgcttcttca ccgacgccgc cttcgcggac gaagccttgt gatggcgatg
    12901 gtaggtggcc cgctgcgtgg atgaagcgtg gctcgcgctc gtcgtccgat agcggccgtt
    12961 gtcccggact cgctgatcgg aatagatgag ctgcccgttc gtgccgtaca cgcgatcgct
    13021 cgacacgacg atcatcgtgg gcaccgcggt cgacaccatg cgcgacggca cgaccgtgcg
    13081 gacgggacga tcgcccgcgg cgaccgccat ccagttctgg cgatactcgg ccggaatggc
    13141 cacgacttca tgagcgccgg tcgaggtctt cacgagagcc accggcactc ccttcgcccc
    13201 ggcacggtaa ctgctcccat cctcgacgag atagaccgtc tcgttggacc ccgaaaggtc
    13261 gtactttggg tcgtccacga tgtaatagat gcgcgtcccg ggaacaaccg ccatgcctga
    13321 ctcgaacacc gtagcaccac gtacggcgga ccaataccgc acgtcgtcgt cgcggtcatt
    13381 ggcccgaact gtcggcgccg cagcgagcaa cgacagggcg gtccccacaa gagctgcagc
    13441 caagatcttc ataggaccca ctcctccttg cgcgtttgct acaggttggg cggcacttgg
    13501 actctgcgtt gcaaacccca tgcccgcggt ctgctaggct cgatgcatga atgcgctgct
    13561 ccggaatctc aggcgcacgc tccggcgatt ccaggatcac gagggacccc gcgtcgccgc
    13621 cgcgatcgcg ttctacgttc tcttctcgtc ggtccccgcg ctcgcgttcc tcatcgcgtc
    13681 cgtcacttgg gtgatccgcg atccgcacga tcaggcgcac atcgtgagcc atctgctcga
    13741 gctcttgccg acgggatcgg agcagaatcg cgcgatgctc atgggaatcg tgaactccgt
    13801 gcggaaggcc agccgcggaa tcaccgtgct cggagcgctg ggtctcgcgt ggtcgagcct
    13861 cgggatgttc agcgcggctc ggtggggact caatcgcgcg tgggggacga aatcgcgcgc
    13921 gaacttcttc ggcgtgcgcc tgatggatct cgccgccgcg gtcgggatct ggatgctcct
    13981 gcttctctcg gccctcgcga ccgcggcggt tcacctcttg gcgggcgagg gtgcgccgtt
    14041 gcccgcgacg tcctggctac cggggctggc gtcgaccgcg atccagagct cgatccccgc
    14101 ggcgctcagc ttcgccgcgt tcctgttcgt ctatcgctac gtgccgagtg tggagcatcg
    14161 gatgagggac gtgttccctg cggcgctcgt cgcgacgtgc gcgttcgagc tcacgaagca
    14221 tctcttcgcg ttctacgtcc gcctcatgac caagagctcg ccgctcttcg ccgcgctcgg
    14281 tgggacgctc ggattcatgc tgtgggtgta tctctgcgcc gcgatcctgc tcctgggggc
    14341 ggagctgggg tacgtgtcga gccggtacgc cctatcgcat gaagtgctgc tggatcagcc
    14401 gaaaggcaag gatcgtcgcc gccagcacag ccgcgagtcg aagcagaaag cctagggtgc
    14461 tgttgttgtg gtagttccgc atgcatcgtg cagaatgcat ggggcgtgcc cgaaattgta
    14521 atttggacaa gcctctggac cgcagcgcct tgcgacgact cgagccacgg ggaagcgacg
    14581 ctaaccggga ggcaaagttc ccgagcctgg gtggaatccc ccagatcagt gcttcggctc
    14641 cggcgcgcac acctcgcatc ccttgtcgaa cacgacccgc caccggccgt ccggctcgag
    14701 gcgccagatc gtgctgaacg tcccgatcag ggtccctttg ggatcgtgca ccggtccgga
    14761 ggtgagcgcg aggttccccg acggaagcac ctcgacggtc tcgggctccc aggagaacgg
    14821 cgcctccttc ccctcgaaga accgcgtcca ggccgccacg accgcggtcc ggccgcgaag
    14881 gggccccttc cccccgaaga agactccctc ctccgcgacg tagcccccga aggcgttgag
    14941 attccggtcg gccatggtct ttgcgaatgc gcgctcggag tcgcgcacct cggacacgag
    15001 gtcgcgcttg tctgcgcctg cggcgcggcc cgactggagc gcgagagcga gcagaacgag
    15061 cgtcagaacg gtgatgcgct tcatggagat cctcccctgc cggcgccgca cgtgagggac
    15121 gcacgggtac gggcgaggat gcaccatcgg cgcgcgcgca tcaattgccc gcatgacgta
    15181 cactgcggtc atgcgcgata acccggaacg gattcccgtc gtcatcgccg agagctacga
    15241 caagctcgcg ctccgcgtgg cgcagcggat cgcggccctc atccgcgagc gtcacaaggt
    15301 gggactcccc accgtgctcg ggctggccac cggatcgact ccgatcggaa tctaccgcga
    15361 gctcatccgc atgcaccgga gcgaggggct cgactggtct cgcgtcgtca cgttcaacct
    15421 cgacgagtac tacccgatgg cgcccgacag cttccacagc taccaccgct tcatgtggga
    15481 gaacctcttc gggcaggtga acatcgatcg gaagaacgtg cacatccccc gcggcgacct
    15541 gccccgcgac caggtggacg cccactgcgc cgagtacgag agccagattc gcgaggcggg
    15601 ggggatcgac ttccagatcc tgggcatcgg gcagacggga catatcgggt tcaacgagcc
    15661 cggctcgagc gcggcgtcga ggacacggct cgtcaccctc gacacgatca cccggcgcgt
    15721 cgcggcgtcg gacttcttcg gcacggagaa cgtcccggtc gaggccgtga cgatgggggt
    15781 cgcgacgatc ctcgaggcga gggagatcgc gctcctcgcg acgggggagc acaaggccgc
    15841 gatcgtcaag cgcgccgtgg agggagacgt cgaccgttcg gtcgcggcca cgtatctcca
    15901 ggggcatcct gccgcgaccg tcttcctcga ttccgcggcc gcggcggagc tgacgcgggt
    15961 caagacgccc tggctgctgg gcgagatcgc gtggaccgaa gcgctcgaga tccaggccgt
    16021 cgtgtggctc gcgggcgaga ccggcaagtc catcttgaaa ttggacgaca tcgactaccg
    16081 ggagcaccgc ctgacgccgc tcctcgcgcg gcacggctcc tcgggggcga tcaacggcct
    16141 cgtgttcaac gcgctgagcg cgaaggtgcg agggcggagc aagctccccg tagggaagcg
    16201 cgtgatcgtc ttctccccgc accctgacga cgacgtgatc tccgccggcg ggacgctccg
    16261 gaagctccac cagaacggaa accacctggt cgtggcctac caaacctccg gcaacatcgc
    16321 ggtgttcgac cacgagctcc ggcgctatct cgattttgtc tgccgcgcct cgcaggactt
    16381 cggcatcgcg agcgaccggc tgccgcgctt cgtggccgag gtggacgcat tcctcaaggc
    16441 gcgaaagccg ggggaggtgg attcgggcga ggtgctgctc ttgaagcgcc gcatccgcga
    16501 agtggaagcg cgctccggcc tcgagacgct cggcatcggc gccgacgcct cgcgattcct
    16561 gaaccttccc ttctaccaga cgggccaggt gcggaaggat ccgatcggag atcgggacgt
    16621 ggcgatcatc caggagctcc tcacggcgga gcggccggat ctgatcctcg tcgccggcga
    16681 tctctccgat ccgcacggca cccaccgcat gtgctacgag gcggtggcgc gcgcgctcga
    16741 gcgctacgat gggaagccgc ccgaggtgtg gctctatcgg ggcgcgtggc aggaatggcc
    16801 gatcgacgag gcggacctcc tcgtgccgat gtccgaggag gagctgcggc tcaagatcag
    16861 cgcgatcttc aagcatcaga gccagaagga ccgcgccccc taccccggcg tggacgagcg
    16921 cgagttctgg cagcgcgtgg aggagcggaa caagggaacg gccgcgctcg tggaccgtct
    16981 cgggctgccg gagtactacg cgatggaagc gctggtcgtc cggagcgcgc gcttgctccc
    17041 ggagtagcgc taggcggggc ggccggcgtc cgcgcccggc ttgtagatga gaccgaggtt
    17101 cttccgtccg ttcatcttca cgatcagcgg gcgctcgaag cgaacgtggc gcaccgcctc
    17161 cgcctcacca agctgtggct gcccctcgat ccagtcccag tcgatgaaac cctcccccag
    17221 atcctcgttc accgtgaagt agcccacgcc gaacgaggtc aggttctgga agaagtggct
    17281 cccctgcgac ggcgtcacgc gcacgtcgcg gaatccggac tccacgatca cccgcgcgcc
    17341 cgaaatctga tcccaggtca cggggatccc gagccaggga tcggtggatc cccagcgtcc
    17401 cactccgatc aggaggtagg gccgcttcgc ctggaggagc tcggcgttga attgggccac
    17461 ggccgacgcg gcgccgcggc tccgcgcgcg atcgaacctg tgaaaatcga cgacgacggc
    17521 gtcccggatg tcgtccacga gcccgttccc catcacctgc gtgctccggc agaggagcgc
    17581 atccttgggc ggctcctcca gctcgaggtt ctcggtctcg tgggccagca cgagggggcg
    17641 catctgtaga aaaccgaagt ggtgcggctt cgacgggtcc ttcgacagga cggtcgcgaa
    17701 ctcgatctcc gcggggcggt tcatgccgcg gcttcccgca tcgaggagga tcgagaggat
    17761 ctcggcgagg gggaagacgc cgtgcttgag aaccggcgcg aaggtcacga ggcgcacgcc
    17821 cgggcgcgcg agtccgtcgt acaccgcctg gttctcggcc gaccacgtgg atccgagggc
    17881 gtagagcgta ccgtccgctt cggcggcttc gagcccgaag cgcttctcgc gcatcccctg
    17941 ctccccgtcg gtatcgcccc cgttcagctc caccgcccag aaatcccgct gcgagttccg
    18001 cacgatctcg tccggcgtcg agaactggag gatggcctga ggatggcgcg ggcagaaggt
    18061 gaggcagcgc tctccctcga ccacgctccg ccccatgccg agcgccaccg cggcgatgcc
    18121 gtcctcggtc tggaggggcg gcgcggcgta atagttgtag gagcgggcca cgcccgagaa
    18181 gtccgggtag aagcgggtcc cgtggtgcgc ccccacgacc cgctggatcg cgacggccat
    18241 cttctcctcc tcgagccggt agggcgtgtt gtggaggaag tccttcgtct gactcaggaa
    18301 ggtcgacgcg tacacgcgcc gcacggcgcc gacgagctgc ctgagccgca cgcgcgggtc
    18361 ggggtggttg ttcgggagca tgaaggtctc gtagacgccg gtgaacggct ggtactgcga
    18421 gtcctcgaga aggctcgagc tccggaccgc gagcggccag tgcgcggact ggacgaacgc
    18481 gcggagcttc cgcatgacgt ccctgggaag gggcgccctc aagaactccg cgaggatctt
    18541 ctcgtcgtcg cggctgttga gcgcgagatc gaggagctcg ttccgctcca ggaaccggtc
    18601 gaagcagtcg gtcgcgagga cgacggtctg cgggacggcg atccgcactc cggggatgcc
    18661 ccgcccgatg ccgaggtagt tgatgaggta gcggacgaac gcgagcccgc gcgctttccc
    18721 gccgagcgag ccgtttccga ttcgcgcgaa gaagttgctg tcgggatcga acgtggcagg
    18781 atcgaaatcg gcgaccagcg cctcggtctg ctcgcggcgg tactcggcga tcgactcgat
    18841 caggttgtgg cggagcccct ccagcgagcc gaagtcctcg acgcgccggg ggcgaagctt
    18901 ccgcgcgagc gcgaactcgg tgcgcgcccc gagccagttg gagaagtggt tccgctgcgc
    18961 gtggtagccg atgctctccg ccggcacggt cttgagcttc tcctcgagcg acttgagatc
    19021 ccgcgcgcgg gccacctcgg tgccgtcggg gagccggaag acgaactcgc cgaacgcaaa
    19081 gttctccacg aggaacgcgc ggagatccga aaggagcgtc tccgagtact tgcgcaggaa
    19141 cgccgccccc acctgagccg cgcccgccgc gaactccgcg cggctcgact ggagcacgat
    19201 cggcagatcc ggaatccggc tccggacgag cttcgcgagg tcgaaccccg cgttccgatc
    19261 gcgcttcccg ccgcgcggga actccacgtc cgtgatcagg cccaggagat agggctcgta
    19321 cttctggacc agctcccacg cctgctcgaa cgtgctcgcg aggaggagct tggggcgagc
    19381 gcgggtgcgc accagcttgt gcgatggatt gatcccttcc gaaatgagcc gctccgactg
    19441 gcggatcagc tccgtgtaga tctctggaag gaaggacgag tagtagcgga cgttgtcctc
    19501 gaccacgagg agcacccgca cgcccatcgc cgccgtgtcg tggaagacgt tccggcggtc
    19561 ctcgacatac ttgacgatcg cgacgaggat ccgggcgttc ccctgccaga ggaagagccc
    19621 gtccagctcc gagacgtcat gcctcgcctc gaattccttc cgctcgtcgt tgtcgtacgc
    19681 catcccgacg accggaatgt cgagccccgc ccgcttcacc tcccgcgcga tctcggtcgc
    19741 atccatgtcc gccgggcgaa gggtgctcag gatgagattg aagcgccgct cggaccgcgc
    19801 gagcgcgagg gcctcatgtc cggaggagac gtgcgtgagc cccggagtgt gatgtagcga
    19861 gagctccagg aactcccccg cgatcagctc attgagccgc ccatcctcct gcagcgtgaa
    19921 cgagtcgtag agactcgaca cgagcaggat gtcctggacc cgaaacggca tgaggtcctg
    19981 gaacgcctgg aacggattgc tcacatcgtg gatgggatcg acttcggaag ggctcacgaa
    20041 ggcatggtgc cacgaggttg gcagcggcga tatcggtttg atgggccggg cggggacgcg
    20101 tggctccccg ccctcacaag cgcgtcccgg cgcaaaaacg cgccgggcgc gaaagttcgg
    20161 gcggggacca cgcgcccccg ccctaccttc acgccgattt agacgaggcc ttggtcgagc
    20221 atcgcgttcg cgaccttcag gaacccgccg atgtttgcgc cgttgagcag atttccgggc
    20281 gtgccgtact tttcggcggt ttcgaagcag gtctggtgaa tggcgaccat gatctggtgc
    20341 aagcgatgat cgacctcgtc ccgcgaccag gctaggcgca tgctgttttg cgacatctcg
    20401 agtcccgagg tggcgacgcc gccggcgttc gctgctttgg cggggccgta gaggatcccg
    20461 gcgtcgaggt agacctggat cgcctcgatg gtgctcggca tgttggctcc ctcggccacg
    20521 agcatgacgc tgttcttgac caggttcgag gcgt
//
LOCUS       DASTKC010000263        14348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_939313, whole genome shotgun sequence.
ACCESSION   DASTKC010000263 DASTKC010000000
VERSION     DASTKC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939313"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFR25_11020"
     CDS             complement(<1..253)
                     /locus_tag="VFR25_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET9941619.1"
                     /translation="MRILERYVLREHLYPFLIGFSVVIFLLTLDFMFDLVDLAIGKGV
                     GAGVVLELFVLSLGYMVALAFPCAVLIACLATFGRLSQDN"
     gene            461..6439
                     /gene="sprA"
                     /locus_tag="VFR25_11025"
     CDS             461..6439
                     /gene="sprA"
                     /locus_tag="VFR25_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HET9941620.1"
                     /translation="MLPRLRLLCILALGLLLGPWARVQAEPYQDLPNPDRLQPIEADL
                     STPGLPSGWRFRPIHANGDYPTGQPDIDVTVDPVAGTVQESYREGDVEVREPLVLTPD
                     EYNQILSGRTVHRLWKDKARSTRSMDRGQLRSGTALRVELPVQLPKLVRTIVGDGTPN
                     IEISGSETITLSGTSDWTASNRIQTERKNQSAFPSFDMKQELNVNLTGSIGDRIKVDI
                     DQSSNVTTSIDNQVKLRYEGGDDDMIRSVELGNTNLSVEGASFRQEGLFGVKTVARLG
                     SVDLQAIASKQQGKTETARFTPSGELKDVQIRDLEYIRRTYYFVADHPVKIRSGTLHV
                     YRDDRNQGNNSTESLGLARVNPLAAPDSASNPEFSGNFTRLTLDEDYFIMTPYITGAT
                     GEIPVIRLRQALGQSEILAVAYVDDLTGQRVGTVENSDFVAPDAALQKPANTLLLKAI
                     GVEANRFTVDPVTSLFDKTNPWFPVLPYELRNFYDLQGRDIAFETLSLKVKRLQNGAV
                     ADPDFIEQGGVKTPLIEILGLDQRTQGTNEVHPDGRIDTQFIDPETGILFFPDLHPFN
                     PTTSDSGCAPGFGGFLCLDNFGRNKLQDETLTNPKVYYSLLPDLSADTRYYIEAEFKS
                     STQGFFLGRFDILEGSETVKVDGIPQRRGADYNIDYQTGQVNFLKPPGPNQTISVDYS
                     FAPGVGSTQLTLLGASASYNPGPNLALTSSVLYDSRGAMETNPKLGEEPAKTVIGDLS
                     SLVIFKPVWMTQLANVIPGVKTNAPSLLNIQGRAAMSMPNPNTAGEAYIDDMEGNRES
                     NTIGLSRTQWFWSSTPLRDMGDSSTTVPVDIQPLVATHSRLQWYNARGVKEHDLKPIL
                     KNEEGGDAERTVVEMNVQLPTGQAVMGPEDWTGITQSISTAGQDFSRLKYMEIWVNDF
                     TQDHALTPGKLHIDFGRVSEDAFWDPDSIPNNVLDTEDTNGDGKLDRGETADVPFEDT
                     GLDHLLDKDEPGYDPVTNPDPNHDDYHFDSGAKILEYSGINGTEGNGEGVPNARPDTE
                     DLNRDGFGDFQNDYFSATIDLSDSSYVAVDVARDYAGESLPDGNTIALDNGWRMFRVP
                     LTPEIFKRVGAANWQNVQHIRLWINGLSQPRKFQVGGIELVGNRWLAQAIDPAKAARG
                     LELGVGVRNNKDDSGAPYFYQAPFAVQNTQGGTASRKEQSLALHHFNLENGDTLIAFK
                     TTTSTNALGWTQYGQIRFWVHGDPNADAQKLRVIARFGADTVNYYEYSAPVPNDSWKN
                     MVVPLDHLSGLKETMEADSMGVRTDFETGAATGEVFRVVGNPSFTRILRISFGTTMVG
                     GPPGPQEGEVWVNDLRLSDVHRDRGVHGEMNVQANFADVLTLNVNYQKEDEDFFRTGS
                     GVNTGTGQNHTATNIATTFNLDKVLPTSGIQLPVRFSVNHTTDVPKFRTGSDVILSGA
                     RSDLETRERNTQTIDLSYRRSGAKRGSFARYTLDALQGALTYARDGSRTTSSIDSSWT
                     FRASGNYDLPIGGLMIGLPILKVGVLPDQLGFSANWEASRDVSYGRQIFEDDDSTTLR
                     SDVKTRLLTVGGRTGWTPLSSVRVNFSITSTRDMLLRNEGGLGFNVGTELVQARTLGL
                     NYTPRWLGLFSPNLAMDGTYRETKNPQQRLLDTDPPDLKSISNGGNARFTAVVPFARL
                     GQRLSRPGHSGASYFNPVRAVISRFQDIQTTFTFTRNTSLSRVTGDAGPAFKTGFTGA
                     FDPDMVRQSNSIFTETRAYTSGANTTFRPTSFITIDARADHRLAFSEAGIGGSRRTLS
                     YALPDLKGRWLDLHRLLGMSETISTMSLNSGYNFRVDEAGPKDAPFDTRTNTTTWGPL
                     LGWDLAWRNGLRANIATNVTQSSQLDQQVYPVTSDRQLVNTDVRFTKTYSAARGIRLP
                     FSRKPMRLPNDLNLNLTFSASSERKVTKRPTLGVADIVEIDQSRWTIGSATNYNFTQT
                     ISGGFNLGFRNQTDRKTSITTRGLTIALNGQFRF"
     gene            6486..7382
                     /locus_tag="VFR25_11030"
     CDS             6486..7382
                     /locus_tag="VFR25_11030"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9941621.1"
                     /translation="MILLTLCSCSRPASAVAFDGKRAFSVLERQCAFGPRNPGSMAHD
                     SCFAYLVSTLKSYAPVVECDTFYYDSPILKKRVPLMNAVARFRPGVKQRVLIGAHWDT
                     RPWADRDPVPAKRHLPILGANDGASGVAVLVELARALRQGHEQPSVGVDLVLFDGEDL
                     GTDADPTGYFRGSQRYVEWKSEETPPIFVVVIDMVGSSTMVLHWEGNSRKMASNIVDL
                     VWEEARTLGVRNFRSDVKHTVYDDHIPFLNAGIPAIDIIDFDYPYWHTTHDTVDRCSA
                     ESLGGVGKVLLSVITRPNLLAN"
     gene            7499..8002
                     /gene="rimP"
                     /locus_tag="VFR25_11035"
     CDS             7499..8002
                     /gene="rimP"
                     /locus_tag="VFR25_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET9941622.1"
                     /translation="MDGNELKEDLSRRIGALLQDESFDLWDLEFSSQAGATVVRVFVD
                     RPSGVTLQDCAYWNKKIGRYLEAENVFRGRYVLEVGSPGLDRTLVRPEHYARFLGASV
                     ELKLHDPLEGRRTFHGEIRVAGPDTVVIADPEAGTVSLPYASIRKARLVVDPWEGMRG
                     QERRRKG"
     gene            8035..9558
                     /gene="nusA"
                     /locus_tag="VFR25_11040"
     CDS             8035..9558
                     /gene="nusA"
                     /locus_tag="VFR25_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET9941623.1"
                     /translation="MNYELMEAMAQIAREKSVDKAILIETLEAGLLSAGRKRFGPSAN
                     IEVRFDDSSGKILMRLKRTVVEDADDLGLEVDLAEAKGVDPDIEIGQELIQDLSLEEL
                     GRNAIAAAKQVLVQRVREAERERIYEDYHDRVGEMVRGTVQQVDRGNIYVKLDRSEGV
                     LPPREQIARDRYHQGDHIKAYIIAVDKLARGPQIILSRTHPEFLRRLFEAEVPEVAEK
                     IIEIKGIAREPGSRAKVSVLSNDDKIDAVGACVGIKGSRVQAIVRELGGEKIDIVPFS
                     HDAIVFVTRALSPAKVLEAQVVDTEQKILVTVADDQLSLAIGKGGQNARLAAKLTGWK
                     IDLISKSERERQREFEKKSRIDIEDLEAAGPKLREKLLREGIETVQDLLKTPFEELLA
                     IQGVGEKTAEKLLEDAKELMAKRGRELEEEATRAAEAEAATDEAESRLGVASIAETEP
                     EPVVELEGGADDRPDEVEPVVDLAPATEGSEEPPLGTAPGEPGTEADFEDEEQAPPK"
     gene            9580..11769
                     /gene="infB"
                     /locus_tag="VFR25_11045"
     CDS             9580..11769
                     /gene="infB"
                     /locus_tag="VFR25_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET9941624.1"
                     /translation="MPAAKRRVYEAAKDMGMSSEALVSILKSLQVEVKSHMSSIDESV
                     VEQVRSKLARDKEAVKEEEARKREKAVQAAKAAAEAQRRVAPAPGLAGLRGGKPAKKK
                     KRAVDEKLIRASVRRTMAEMEGTRRRRHHHKTEEGAAVGVEEAPRVIRVSEFISVAEL
                     AGHLEVKPQEVIAVCMQLGIMANINRRLDKDSIQAVADEFGFEVEFVSELGEEVEEVE
                     VESAEGLTARPPVVTIMGHVDHGKTSLLDYIRKTNVIAGEAGGITQHIGAYEVELPSR
                     DRITFLDTPGHEAFTAMRARGAQVTDIVVLVVAADDRVMPQTVEAIDHARAANVPMII
                     AINKIDLPDANIEMVRQDLSKHNLLVEEWGGKTIAVPISAKKGTNVEKLLEMILLQSE
                     LLELKADASKHARGVVIESRVEQGRGTIASVLVQKGTLRVGDAFVAGSTSGKVRAMLN
                     ERGSRVETAGPSTPVEVLGWSSPPQAGDTFVVMEDEREARELASKRGQLQREQEHRLF
                     KHVTLTDLYSQIKAGKVSDLLVVLKGDVDGSVEALEDSLTKLSTDEVKLRVIHRGVGQ
                     ITESDVLLAAASDAVIIGFHVKPDQRAAELASKEKVDVRLYDIIYEAVADVKDAMSGL
                     LAPEIRETATGSAEVRQVFRTTRSGVIAGCMVLSGNIQRSARARLVREGQVVWDGKIA
                     SLRRFKDDVREVASGYECGIGLEGRDDIREKDLIESYVLEEVARRLS"
     gene            11771..12052
                     /locus_tag="VFR25_11050"
     CDS             11771..12052
                     /locus_tag="VFR25_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET9941625.1"
                     /translation="MRVGTLEVDLHLPASDSLKAKRSILNHVKDRVRSRFNAAVAEVG
                     PLDLWQRASLGVAVVGAEPVLLERVLRDVLAAIEREDRLAVLDYHIQVD"
     gene            12071..12433
                     /gene="rbfA"
                     /locus_tag="VFR25_11055"
     CDS             12071..12433
                     /gene="rbfA"
                     /locus_tag="VFR25_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9941626.1"
                     /translation="MPQRESPRTRRVADRIVEELAECLASKVEDPRLRTLSVTGARIS
                     RDLSAARVWVSGTMTAEEEPGVLKALAHAAPFLRSYLAPRLGLRIVPTLTFVIDKSIE
                     TGTRIEQLLRDLERPEGG"
     gene            12430..13428
                     /locus_tag="VFR25_11060"
     CDS             12430..13428
                     /locus_tag="VFR25_11060"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET9941627.1"
                     /translation="MTAIVHDRFQAFLEAHREFLILSHVDPDADAIGSCLALARVIRG
                     MGKGTAVVNDSPLPDSLRFLPDSSQVLRPSDVLGREFDAAFVLDCSSLDRVGPDAARL
                     VVAGMPVANVDHHAANDGFGDPRLVNVEASATAELIHEILEACGLPIDADTAECLYAG
                     VASDTGAFRHPNTTPRALRLAARLVERGARPALTAEALYGRKTEASLRILGMALTSLE
                     SRSGGQVGVITLSRDMFERARATSEDADGIVQFAKSLVGTRVGLLVQEVAPGEVRLSF
                     RSDGTVDVNEVAARFGGGGHKNAAGARVKGDLRSIHDQALEALDKAVNGGFPPSAG"
     gene            13400..14332
                     /gene="truB"
                     /locus_tag="VFR25_11065"
     CDS             13400..14332
                     /gene="truB"
                     /locus_tag="VFR25_11065"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9941628.1"
                     /translation="MADSLHLLDKPEGWTSHDVVARMRTVLGERRVGHAGTLDPFASG
                     LLLLGEGRATGILGCLGLLPKRYHARALLGVTTDTQDRTGKVLGRSNDIPRREDVAHA
                     LDSFRGPLRQRPPLYSAVKVRGERLYESARRGVEVEREHRPIQVYALELIQADPPEVV
                     LDVTVSRGTYVRTLAHDLGEALGCGAHLTELRRTASGPFTVDQALSCDRDRNPGREAF
                     RERSLAPEDALAFLPRVRLTEDEASRLRHGQAPVFDPARVEATAAAWPLPPGAAGWPL
                     SLLGPDGALLGIAHASEEQTSGLTIRLLRVLAEA"
BASE COUNT         2604 a         4820 c         4796 g         2128 t
ORIGIN      
        1 cgttgtcctg ggagagccgg ccgaaagtgg cgaggcacgc gatgaggaca gcgcagggga
       61 acgccagggc caccatgtag cccagggaca gcacgaagag ctcgagcacc acgccggctc
      121 caaccccttt gccgatggcg aggtcgacca gatcgaacat gaagtccagc gtcagcagga
      181 agatgaccac gctgaacccg atcaggaacg ggtacaggtg ctcgcggagg acgtatcgtt
      241 cgagtattct catccgtctt tcggggcggg tcgaggtggg cgtcgcacgc ggccgcagta
      301 tagggagccg gggcgcgtcc tggcaaacct tccgacctcc catccagagg cgccgcattt
      361 acttccggct cgaactccgc acgagaccag gggtttaagt accagcatcg actccccctt
      421 gtcgagccac ccaaggaacg gaatactccg cgatggacgc atgcttcctc gtttgaggtt
      481 gttgtgcatc ctcgctctgg gcctcctttt agggccttgg gcgagggttc aggccgaacc
      541 ctaccaggac ctgcccaacc ccgaccggct gcagccgatc gaggccgatc tcagcacgcc
      601 cggcttgccc tcggggtgga ggttccgccc catccacgcc aacggcgact acccgacggg
      661 ccagcccgac atcgacgtca ccgtcgaccc ggtcgcgggg accgtccagg aaagctatcg
      721 ggaaggggac gtcgaggtcc gcgagcctct cgttttgacc cccgatgagt acaaccagat
      781 cctgagcggc cgcaccgtcc accgcctctg gaaggacaag gccaggtcca cccgctccat
      841 ggatcgcggc cagctccgat cgggcaccgc gcttcgcgtg gagcttcccg tccagcttcc
      901 caagctcgtc cggaccatcg tgggcgacgg gacccccaac atcgagatct cgggaagcga
      961 gacgatcacc ttgagcggga ccagcgactg gaccgcgagc aaccggatcc agaccgagcg
     1021 caagaaccag agcgcgttcc ccagcttcga catgaagcag gagctgaacg tgaacctgac
     1081 cggctcgatc ggcgaccgca tcaaggtcga catcgatcag tcgagcaacg tcacgacgtc
     1141 gatcgacaac caggtgaagc tccggtacga gggcggcgac gacgacatga tccgctccgt
     1201 cgagctcgga aacacgaacc tgtcggtgga aggcgcttcg ttccggcagg agggactctt
     1261 cggcgtcaag accgtggccc ggctcggcag cgtggacttg caggcgatcg cgagcaagca
     1321 gcaaggaaag accgagaccg cgcgcttcac tccgtccggc gagctcaagg acgtgcagat
     1381 ccgggacctc gagtacatcc gccggacgta ctacttcgtc gcggatcatc cggtgaagat
     1441 ccggtccggg accctgcacg tgtacaggga cgaccggaac cagggcaaca acagtacgga
     1501 gagcctcggt ctcgcccggg tcaacccact cgcggcgccg gactcggcgt cgaatccgga
     1561 gttctcgggg aacttcacca gactcacgtt ggacgaagat tacttcatca tgactccgta
     1621 catcacgggt gcgaccgggg agattcccgt catccgtctg cgccaagcgc tcggccagtc
     1681 ggagatcctg gccgtggcct acgtggacga cctcacgggc caacgggtgg gcaccgtgga
     1741 gaacagcgac ttcgtggcgc ccgatgcggc cctccagaag cccgcgaaca cgctcctcct
     1801 gaaggcgatc ggtgtcgagg cgaaccggtt cacggtcgat ccggtcacct ccctgttcga
     1861 caagacgaat ccctggttcc cggtactccc gtacgagctc cgcaacttct acgacctcca
     1921 gggtcgcgat atcgcgttcg agaccctgag tctcaaagtg aaacgactcc agaacggtgc
     1981 ggtcgccgac ccggacttca tcgagcaggg cggggtgaag acgccgctca tcgagatcct
     2041 ggggctcgat cagcggaccc aggggacgaa cgaggtccat ccggacgggc gcatcgacac
     2101 ccagttcatc gatcccgaga cgggcatcct gttcttcccc gacctccacc ccttcaatcc
     2161 gaccacctcg gactccgggt gcgcccctgg gttcggtggg ttcctctgcc tcgacaactt
     2221 cggccggaac aagctccagg acgagaccct cacgaatccg aaggtctact actcgctcct
     2281 gccagacctg agcgccgaca cccgttatta catcgaggcc gaattcaaga gcagcaccca
     2341 ggggttcttc ctcgggcgct tcgacatcct cgaaggatcg gagacggtca aggtcgacgg
     2401 catcccgcag cgccgcggcg ccgactacaa catcgactac cagacggggc aggtgaactt
     2461 cctgaagcct cccggtccca accagacgat cagcgtcgac tactcgttcg cgccgggcgt
     2521 cggctcgacc cagctgacgc tcctcggcgc ctcggcttcc tacaacccgg gccccaacct
     2581 ggccctgacc tcgagcgtgc tctacgacag ccgcggggcc atggagacca atccgaagct
     2641 cggggaagag ccggccaaga ccgtcatcgg cgacctctcc tcgctcgtca tcttcaagcc
     2701 ggtctggatg acgcagctcg cgaacgtgat cccgggcgtc aagaccaacg ctccatccct
     2761 cctcaacatc caggggcgcg ccgcgatgag catgccgaac ccgaacacgg cgggggaggc
     2821 ctacatcgac gacatggaag ggaaccgcga gtccaacacg atcgggctga gccgcacgca
     2881 gtggttctgg agctcgaccc cgctcaggga tatgggagac tcgtcgacca ccgtacccgt
     2941 cgatatccag ccgctcgtag cgactcacag ccggctccag tggtacaacg cgcgaggcgt
     3001 gaaggagcac gacctcaagc ccatcttgaa gaacgaggaa gggggcgacg ccgagcgcac
     3061 ggtggtcgag atgaacgtcc agcttcccac cgggcaggcg gtcatggggc ccgaggactg
     3121 gacgggcatc acccagtcca tctccaccgc ggggcaggat ttctcacggc tcaagtacat
     3181 ggagatctgg gtcaacgact tcacccaaga ccacgcgctg actcctggga agctccatat
     3241 cgacttcggc cgcgtgagtg aggacgcttt ctgggacccg gattccatcc cgaacaatgt
     3301 gctcgacacc gaagacacca acggcgacgg caagctggac cgaggcgaga cggcggacgt
     3361 gccgttcgag gacacggggc tcgatcacct cttggacaag gacgagccgg gctacgaccc
     3421 ggtgaccaac ccggatccga accacgacga ctaccacttc gattcgggag ccaagatcct
     3481 cgaatacagc gggatcaacg gcacggaagg aaacggtgaa ggcgttccca acgccaggcc
     3541 ggacaccgag gacctgaacc gggacgggtt cggcgacttc cagaacgact acttctcggc
     3601 gaccatagac ctctccgatt cgtcgtacgt cgcggtggac gtggcacgcg actatgcggg
     3661 agagagcctg ccggacggga acacgatcgc gctcgacaac gggtggcgga tgttccgcgt
     3721 gccgctgact cccgagatct tcaagcgtgt cggggctgcg aactggcaga acgtgcagca
     3781 catccggctc tggatcaacg gtctctccca gccgcgcaag ttccaggtcg gcggcatcga
     3841 gctcgtgggg aaccgctggc tggctcaggc gatcgacccc gccaaggcgg cgcgagggct
     3901 ggagctcggc gtgggcgtcc ggaacaacaa ggacgacagc ggcgctccgt atttctatca
     3961 ggcgccgttt gcggttcaga atacccaggg cggcacggcg agccgcaagg agcagtcgct
     4021 cgcgctccac cacttcaacc tcgagaacgg cgacaccctc atcgccttca agaccacgac
     4081 ctcgaccaac gcgctgggct ggacgcagta cgggcagatc cgcttctggg tgcacggcga
     4141 tcccaacgcg gacgcgcaga agcttcgcgt tatcgcccgg ttcggcgcgg acaccgtgaa
     4201 ctactacgag tacagcgccc cggtcccgaa cgattcgtgg aagaacatgg tcgtcccgct
     4261 cgaccacctg tcgggcctca aggaaaccat ggaggccgac tccatgggag tccgaaccga
     4321 tttcgagacc ggagcggcca cgggcgaggt cttccgcgtc gtcggcaatc caagcttcac
     4381 gcgcattctc cgcatcagct tcggcacgac catggtcggg gggccgcccg gtccgcagga
     4441 gggagaggtc tgggtcaacg acctccgcct gtccgatgtg caccgcgacc ggggcgtcca
     4501 cggggagatg aacgtccagg ccaacttcgc ggacgtcctc acgctgaacg tcaactacca
     4561 gaaggaggac gaggacttct tccgcacggg ctcgggggtg aacaccggga ccggccagaa
     4621 ccacaccgcc acgaacatcg ccacgacgtt caatctcgac aaggtgcttc cgacgagcgg
     4681 gatccagctc cccgtccggt tctcggtgaa ccacacgacc gacgtgccca agttccgcac
     4741 cggctcggac gtgatccttt ccggcgcccg ctcggacctc gagacgcgcg agcggaacac
     4801 acagacgatc gacctgagct accgtagatc cggagcgaag cgggggagct tcgcgcgcta
     4861 caccctggat gcgctccagg gcgccctcac gtacgcgcgc gacggatcgc gcacgacgag
     4921 cagcatcgac tcgagctgga ccttccgggc ctcgggcaac tacgacctcc cgatcggcgg
     4981 gctcatgatc gggctcccga tcctcaaggt cggcgtcctc ccggaccagc tcgggttcag
     5041 cgccaactgg gaggcttccc gcgacgtcag ctacggccgg cagatcttcg aagatgacga
     5101 ttcgacaacg ttacgctcgg acgtgaagac gcgcctcctc acggtgggag gcaggaccgg
     5161 ctggacgccg ctctcatccg tgcgcgtgaa cttcagcatc acgtccaccc gcgacatgct
     5221 gctccggaac gaggggggcc tcggattcaa cgtggggacg gagctggtcc aggcgcggac
     5281 gctcggcttg aactacacgc cgcgatggct gggtctcttc tcacccaatc tggcgatgga
     5341 cgggacctat cgtgagacga agaacccgca gcagcggctc ctcgacaccg atccgcccga
     5401 cctcaagtcg atctccaacg gggggaacgc gcggttcact gcggtggtcc cgttcgcacg
     5461 gctgggacag cggctctcgc gccctggaca ctcgggggcc tcgtacttca atccagtgcg
     5521 cgccgtgatc tcccgattcc aggacatcca gaccacgttc accttcacgc gcaacaccag
     5581 cctctcgcgc gtgacggggg acgccgggcc ggcgttcaag accgggttca ccggcgcgtt
     5641 cgatccagac atggtccgcc agtccaactc gatcttcact gagacgcgcg cctacacgtc
     5701 cggggcgaac acgaccttcc gtcccacgtc cttcatcacg atcgacgcgc gggcggacca
     5761 caggctcgcg ttcagcgagg cgggcatcgg aggatcgcgg cggacgctct cctacgcgct
     5821 tccggacctg aaggggcggt ggctcgacct ccatcgcttg ctcggcatgt ccgagaccat
     5881 cagcacgatg tcgctcaatt cggggtacaa cttcagggtc gacgaagcgg gccccaagga
     5941 cgcccccttt gatacgcgca cgaacacgac cacctggggc ccgctcctgg gatgggatct
     6001 cgcctggcgc aacggactcc gcgccaatat cgcaaccaac gtgacgcaga gctcacaact
     6061 ggaccagcag gtctaccctg tgaccagcga caggcagctg gtcaacacgg acgtccggtt
     6121 caccaagacc tactcggccg cgcgcgggat ccggctgccg ttctcgagga agccgatgcg
     6181 gctcccgaac gacctcaacc tgaacctcac attctcggcc tcgtcggagc gcaaggtcac
     6241 gaagcgccca accctgggcg tggcggacat cgtggagatc gatcaatctc ggtggacgat
     6301 cgggagcgcc acgaattaca atttcaccca gaccatctcc ggcggattca acctcgggtt
     6361 ccgcaaccag accgaccgga agacgagcat caccacccgc ggcttgacca ttgccctcaa
     6421 cggccagttc cgattctagt cgtccggtaa tctcgacgcg cgcgattcac gcgctcgccg
     6481 cggtcgtgat tctgctcacc ctttgttcct gcagccgtcc cgcctccgcg gtcgccttcg
     6541 acggcaagcg cgcgttcagc gtcctcgagc ggcagtgcgc ctttggaccg cgtaaccccg
     6601 ggagcatggc gcacgactcc tgcttcgcct acctggtctc caccctcaag agctacgcgc
     6661 cggtcgtgga gtgcgacacc ttctattacg actcacccat cttgaagaag cgggtcccgc
     6721 tcatgaacgc ggtcgcgcgc ttccgcccgg gggtcaagca gcgcgtcctc atcggggcgc
     6781 actgggacac gcgcccctgg gccgatcgcg atcccgtccc cgcgaagcgc catctcccga
     6841 tcctgggcgc caatgacggc gcctccggcg tcgccgtcct ggtcgagctg gcgcgggcgc
     6901 ttaggcaggg gcacgagcag ccgtccgtcg gggtggacct cgtgctcttc gacggggagg
     6961 atctgggaac cgacgccgat ccgaccggct acttccgggg cagccagcgg tacgtcgagt
     7021 ggaagtccga ggagacgccc cccatcttcg tggtggtcat cgacatggtg gggtccagca
     7081 ccatggtgct ccactgggag ggaaactccc ggaagatggc ctcgaacatc gtggacctgg
     7141 tctgggagga ggctcggacc ctcggggtcc gtaacttccg ctcggacgtc aagcacaccg
     7201 tctacgacga ccacataccc ttcctcaatg ccgggatccc ggcgatcgac atcatcgact
     7261 tcgactaccc ctactggcac accacccacg acaccgtgga ccgatgctcg gccgagagcc
     7321 tgggcggggt cggcaaggtc ctcctaagtg tcattaccag acccaacttg ctcgccaact
     7381 gagattgccc ttgaaccttc cggcatgagc cggtaggttg gggggtccga gtgggccagg
     7441 ggtccactcg tactttttcg gccccggccc ggggtcggga gtcccggggg gctgccgcgt
     7501 ggacggtaac gaactgaagg aggacctgag ccggcgcatc ggggccctcc tgcaagacga
     7561 gtcgttcgat ctctgggacc tggagttctc gtcccaggca ggcgcgacgg tcgtccgggt
     7621 gttcgtggac cggccgtccg gcgtcaccct ccaagactgc gcctactgga acaagaagat
     7681 cgggcggtac ctggaagcgg agaacgtgtt ccggggacgc tacgtgctgg aggttgggtc
     7741 gcccgggctg gaccggacgc tcgtgcggcc ggagcactac gcgcggttcc tgggcgcgag
     7801 cgtggagctg aagctccacg acccgctcga ggggcgccgg acgtttcacg gcgagatccg
     7861 ggtcgcgggt ccggacacgg tggtcatcgc cgatcccgag gccgggacgg tgagcctgcc
     7921 gtacgcgtcg atccggaagg cgcggttagt cgtcgaccca tgggaaggga tgcgcggcca
     7981 ggagcgccga cggaaagggt gaagacgttg gcacgcgagc ctcaaatgcg aaacatgaac
     8041 tacgagctga tggaagcgat ggctcagatc gcgcgcgaga agagcgtcga caaggccatc
     8101 ctcatcgaga cgctcgaggc cggcctcctc tccgcgggga ggaagcggtt cggcccgagc
     8161 gcgaatatcg aagtgcggtt cgacgattcg agcggaaaga tcctgatgcg cttgaagcgg
     8221 acggtcgtcg aggacgccga cgatctgggt ctcgaggtgg atctcgccga ggccaagggc
     8281 gtcgatccgg acatcgagat cggccaggag ctgatccagg acctctcgct ggaggagctg
     8341 gggcggaacg cgatcgcggc cgccaagcag gtcctggtcc agcgcgtgcg cgaagcggag
     8401 cgcgagcgga tctacgagga ctaccacgac cgcgtcggcg agatggtgcg cggcacggtg
     8461 cagcaggtgg atcgcggcaa catctacgtg aagctcgacc gctcggaggg cgtgctgccc
     8521 ccgcgggagc agatcgcgcg cgaccgctac caccagggcg accacatcaa ggcgtacatc
     8581 atcgccgtgg acaagctcgc ccgcgggccg cagatcatcc tgtcgcggac gcaccccgag
     8641 ttcctccggc ggctgttcga ggccgaggtg ccggaggtgg cggagaagat catcgagatc
     8701 aagggcatcg cccgcgagcc cggctcgcgc gcgaaggtgt ccgtgctctc gaacgacgac
     8761 aagatcgacg ccgtgggagc ctgcgtcggc atcaagggat cgcgcgtgca ggcgatcgtg
     8821 cgggagctgg ggggcgagaa gatcgatatc gttcccttca gccacgacgc gatcgtcttc
     8881 gtcacccgcg ccctgagccc ggccaaggtg ctcgaggccc aggtggtcga tacggagcag
     8941 aagatcctgg tcacggtcgc ggacgaccag ctctcgctcg ccatcggcaa ggggggccag
     9001 aacgcgcgcc tcgcggcgaa gctgaccggt tggaagatcg atctcatctc gaagagcgag
     9061 cgcgagcggc agcgggagtt cgagaagaag tcgcgcatcg acatcgagga cctggaggcg
     9121 gctggaccca agcttcggga gaagctcctc cgggagggga tcgagacggt gcaggacctc
     9181 ctcaagacgc cgttcgagga gctgctcgcg atccaggggg tcggcgagaa gacggccgag
     9241 aagctcctcg aggatgccaa ggagctcatg gcgaagcgcg gccgggagct ggaagaggaa
     9301 gccacgcggg ccgcggaggc cgaggctgcg accgatgagg ccgagtcgcg gctcggcgtt
     9361 gcgtcgatcg cggagaccga gcccgagccc gtcgtggagc tcgagggggg cgcggacgat
     9421 cgcccggacg aggtcgagcc ggtcgtggat ctggcccccg caacggaagg aagtgaagag
     9481 ccgcccctgg gaacggcgcc gggcgaaccg ggcaccgagg cggacttcga ggacgaggaa
     9541 caggcgcccc ccaagtaggg cggcgggaaa cgagggtcga tgccagcggc aaaacgacgg
     9601 gtgtacgaag cggcgaagga catggggatg tcgagcgaag cgctcgtcag cattctcaag
     9661 tcgctccagg tcgaggtgaa gagccacatg agctcgatcg acgagagcgt cgtcgagcag
     9721 gtgcgctcga agctcgcgcg cgataaggag gccgtcaagg aagaggaggc ccggaagcgc
     9781 gagaaggcgg tccaggcggc gaaggccgcg gccgaggctc agcgccgcgt ggcgccggcg
     9841 cccggactgg ccggacttcg cggcggcaag cccgcgaaga agaagaagcg cgccgtggac
     9901 gagaagctca tccgggcgtc ggtccgtcgc acgatggccg agatggaagg cacgcgccgc
     9961 cgccggcacc accacaagac cgaggaaggc gctgccgtcg gcgtcgagga ggcgccgcgc
    10021 gtcatccgcg tgagcgagtt catctccgtc gccgagctcg ccggacatct cgaggtcaag
    10081 ccgcaggaag tcatcgcggt ctgcatgcag ctcgggatca tggcgaacat caaccgccgg
    10141 ctcgacaagg actcgatcca ggcggtggcc gacgagttcg ggttcgaggt cgagttcgtc
    10201 tccgagctgg gcgaggaggt cgaggaggtc gaggtcgaga gcgcggaggg tctcaccgcg
    10261 cgtccccccg tggtcacgat catggggcac gtcgatcacg gcaagacgtc gctcctcgac
    10321 tacatccgga agaccaacgt catcgccggc gaggcgggcg gcatcacgca gcacatcggc
    10381 gcgtacgagg tcgagcttcc ctcgcgcgat cgcatcacgt tcctggacac gccgggccac
    10441 gaggcgttca cggccatgcg cgcccgcggg gcgcaggtga ccgacatcgt tgtgctcgtc
    10501 gtggcggcgg acgatcgcgt catgccgcag accgtcgagg cgatcgacca cgcgcgcgcg
    10561 gccaacgtgc cgatgatcat cgcgatcaac aagatcgatc tccccgacgc caacatcgag
    10621 atggtccggc aggatctgtc gaagcacaac ctgctggtgg aggagtgggg cggcaagacg
    10681 atcgccgttc cgatctccgc caagaaggga accaacgtcg agaagctcct cgagatgatc
    10741 ctcctccagt cggagctcct cgagctcaag gcggacgcgt cgaagcacgc gcgcggcgtg
    10801 gtcatcgagt cgcgcgtgga gcaggggcgc gggaccatcg cgagcgtgct cgtgcagaag
    10861 gggacgctcc gggtcggcga tgcgttcgtg gccgggtcca cgagcggcaa ggtgagggcc
    10921 atgctgaacg agcgcggctc gcgcgtcgag accgcgggac cgtcgacgcc ggtcgaggtg
    10981 ctcggctgga gctccccgcc ccaggcgggg gacacgttcg tcgtgatgga ggacgagcgg
    11041 gaggcgcgcg agctggcgtc gaagcgcggc cagctccagc gcgagcagga gcaccgcctc
    11101 ttcaagcacg tcacgctgac cgacctctac agccagatca aggccggcaa ggtgagcgat
    11161 ctgctcgtcg tcctgaaggg cgacgtggac ggatcggtgg aggcgctcga ggactcgctg
    11221 accaagctct cgaccgacga ggtgaagctc cgcgtcatcc atcgcggcgt cggacagatc
    11281 accgagtccg acgtgctcct cgcggccgcg tcggatgccg tgatcatcgg gttccatgtg
    11341 aagccggacc agcgcgccgc cgagctcgcg tcgaaggaga aggtcgacgt ccggctctac
    11401 gacatcatct acgaggccgt cgccgacgtg aaggacgcca tgtccggcct cctcgcgccg
    11461 gagatccggg agacggcgac cggctcggcc gaggttcgtc aggtgttccg gacgaccagg
    11521 tcgggcgtca tcgccggctg catggtcctc tcggggaaca tccagaggag cgcccgcgcc
    11581 cggctcgtgc gtgaggggca ggtcgtgtgg gacggcaaga tcgcctcgct ccgccgcttc
    11641 aaggacgacg tccgcgaggt ggcgtccggc tacgagtgcg gcatcgggct cgagggccgc
    11701 gacgacatcc gcgagaagga cttgatcgaa tcctatgtcc tcgaggaagt cgcgcgacgg
    11761 ctttcgtgag atgcgggtgg gcaccctcga ggtggacctg caccttccgg cgtccgattc
    11821 cctgaaggcc aagcgctcga tcttgaacca cgtgaaggac cgcgtgcggt cgcgcttcaa
    11881 cgcggccgtg gccgaagtgg ggccgctcga tctctggcag cgcgcctcgc tcggcgtggc
    11941 ggtcgtcgga gccgagccgg tactcctcga gcgcgtgctt cgggacgtgc tcgccgccat
    12001 cgagcgcgag gatcgcctcg ccgtgctcga ctaccacatc caggtcgact gaaccaaccg
    12061 gacggaaccc atgccccagc gtgaaagccc aaggacgcgc cgcgtcgcgg accgcatcgt
    12121 cgaggagctg gcggagtgtc tcgcgtcgaa ggtcgaggat ccgagactcc gcacgctctc
    12181 ggtgacgggg gcgcgcatct cgcgcgacct ctccgccgcc cgcgtctggg tgagcggcac
    12241 gatgaccgcc gaggaggagc cgggcgtcct gaaagcgctc gcgcacgccg cgccgttcct
    12301 ccgctcctac ctcgcgccga ggctcgggct tcgcatcgtg cccacgctca cgttcgtgat
    12361 cgacaagtcg atcgagaccg ggacgcggat cgagcagctc ctgcgcgacc tcgagcgtcc
    12421 ggaaggcggg tgaccgcgat cgtgcacgat cgcttccagg cattcctcga agcccaccgc
    12481 gagttcctga tcctctccca cgtcgatccc gacgccgacg cgatcggatc ctgcctcgcg
    12541 ctcgcgcggg tgatccgcgg gatgggcaag ggaacggcgg tggtgaacga ctccccgctc
    12601 ccggacagcc ttcgcttcct gccggactcg agccaggtcc ttcgaccgtc ggatgtcctc
    12661 ggacgcgagt tcgacgcggc gttcgtgctc gactgctcga gcctcgaccg cgtcggaccc
    12721 gatgcggcgc gcctggtcgt ggccggcatg cccgttgcca acgtcgacca ccacgcggcg
    12781 aacgacggct tcggcgatcc gcgcctcgtc aacgtggagg cgagcgcgac ggcggagctg
    12841 atccacgaga tcctcgaggc ctgcgggctc ccgatcgacg ccgacaccgc ggagtgcctc
    12901 tacgccggag tcgcgtcgga cacgggcgcc ttccggcatc cgaacaccac cccgcgcgcg
    12961 ctccgactcg cggctcgcct cgtcgagcgc ggtgctcggc cggcgctcac cgcggaagcg
    13021 ctctacggcc ggaaaacgga agcgagcctc aggatcctcg gcatggcgct cacgtcgctc
    13081 gagtcgcggt cgggaggcca ggtcggggtg atcacgctca gccgcgacat gttcgagcgc
    13141 gcacgcgcga cgtcggagga cgcggacggc atcgtccagt tcgcgaagtc gctcgtcggc
    13201 acgcgcgtcg gtctcctcgt gcaggaggtc gcccccggcg aggtccggct cagcttccgc
    13261 tccgacggaa cggtggacgt gaacgaggtg gcggcgcggt tcggcggcgg cggacacaag
    13321 aacgcggccg gagctcgcgt gaaaggcgac ctccgatcca tccacgatca ggcactcgag
    13381 gccctcgaca aggcggtgaa tggcggattc cctccatctg ctggataagc cggaaggctg
    13441 gacctcgcac gacgtggtgg cgcggatgcg caccgtgctc ggcgagcgtc gcgtgggcca
    13501 tgcgggcacg ctcgatccct tcgcgagcgg actcctcctc ctcggcgagg ggcgggcgac
    13561 cggcatcctc ggatgcctcg gtctcctccc caagcgctac cacgcgcgcg cgctcctcgg
    13621 cgtgaccacc gacacgcagg acaggaccgg caaggtcctg gggcgttcca acgacatccc
    13681 tcgccgcgag gacgtggccc atgcgctcga ttcgttccgg ggcccgctcc gtcagcggcc
    13741 gcccctctac tcggccgtca aggtgcgcgg cgagcgcctg tacgagtcgg cgcggcgtgg
    13801 ggtcgaggtg gagcgcgagc atcggccgat ccaggtgtac gcgctcgagc tgatccaggc
    13861 ggatccgccc gaggtcgtcc tcgacgtcac cgtgagtcgc ggcacgtacg tgcgcacgct
    13921 cgcgcacgat ctcggtgagg ccctcggctg cggcgcgcat ctgacggagc ttcgccgcac
    13981 ggcgagcggt cctttcacgg tggaccaggc gctctcgtgc gatcgcgatc ggaaccctgg
    14041 acgcgaggcg ttccgcgagc gctcgctcgc gccggaggac gcgctggcgt tccttccgcg
    14101 cgtccgcctc accgaggacg aggcctcgcg gcttcgccac ggccaggcgc ccgtgttcga
    14161 ccccgctcga gtcgaggcga cagcggccgc gtggccgctc cctccgggcg ccgcggggtg
    14221 gccgctctcg ctcctcggcc ctgacggcgc gctccttgga atcgctcacg cctccgagga
    14281 gcagacgtcc ggcctcacga tccgcctcct tcgagtgctc gcggaggcat gacccagacc
    14341 gcgcgtcc
//
LOCUS       DASTKC010000264        10534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_703456, whole genome shotgun sequence.
ACCESSION   DASTKC010000264 DASTKC010000000
VERSION     DASTKC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703456"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFR25_11070"
     CDS             complement(<1..972)
                     /locus_tag="VFR25_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941629.1"
                     /translation="MTNQLTVLVTNVNNLPITYHWSAASGTLLDSTSATVNWQAPDSV
                     GTYDVTASIESQDGDAHFFKTTTFHIFVDNEYDRWTHAEEVQFDPAPVPAPTGGIVFA
                     QYSNITNGQSDIWYIPGPGLAPEQRTTGFFTANAPTMKADGSVLAFAARPTSDDSQHV
                     YISAPAGGTPDPAVSQVLTTATAQSHLFGNPRFSRTVGWLMYNSDSGQATAFVPRVLY
                     RDIPVVPPAVLPAPERAIEDQSFGSRTFWMPNWGPDIDADGKPDSIVTMSFRFFRAAN
                     QVSNGLYKFPTNPPATSAVQWLADSSAAEPDWSADGQYIVFTDRSTPN"
     gene            complement(1199..1693)
                     /locus_tag="VFR25_11075"
     CDS             complement(1199..1693)
                     /locus_tag="VFR25_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9941630.1"
                     /translation="MQLSELINENAIRLHLAARDKESCIKELVSLLESAHGVNTKGEI
                     LSKVLQRESMMSTGIGNGVAIPHGKTRLLDHLVAACGVAPAGIEFDSMDGEPATLFIL
                     LVSPESLRGPHVKALANVSRLLKEESVRNALRTSATPEAFLGVLREAESRYLLEPPSP
                     PGRA"
     gene            complement(1843..4467)
                     /gene="clpB"
                     /locus_tag="VFR25_11080"
     CDS             complement(1843..4467)
                     /gene="clpB"
                     /locus_tag="VFR25_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET9941631.1"
                     /translation="MRFDKFTTKSQEALAEAQRLAERAGHPEVGTEHVLQALLDQKGG
                     AVVPVLEAARVPVDRIRRALEQRFQSLPKVSGGRQTVLSPSVASMLEHAEVEAERLRD
                     EYVSTEHFLLGALDPAVTSEMGRILREAGATRENVYHALLSVRGGEQVSTPDPEETYQ
                     ALTKYARDLTEAARKGSLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLAQRIVSGDVPEGLKSRRVLALDLGALLAGAQYRGQFEERLKSVLKEVQRSEGQVIL
                     FIDELHTLVGAGAAEGALDASNLLKPALARGELHCIGATTLDEYRKRIEKDAALERRF
                     QPVFVSEPTVEQTIAILRGLRERYENFHKVKIQDAALVAAAILSNRYLTDRQLPDKAI
                     DLVDEAASRLRIEMDSMPSEIDTLERKKRQLEIERLALKREKDDGAKTRRALIEEEIA
                     ETETELKRLTDHWGREKAAVQKINEGRARMEELRNEEQQAERGGDLERVARIRHGDVP
                     KVRKQLDQDTAALAVIQKDLKLLKEEVDEEDIAEVVARWSGIPVQRLLESELAKLLHM
                     EERLTERVVGQDQAVRAVSDAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELARALAE
                     FLFDDERAMVRVDMSEYQEKHTVARLIGSPPGYVGYEEGGQLTEAVRRRPYAVILLDE
                     IEKAHADVTNVLLQIFEDGRLTDGQGRTVDFKNTLVIMTSNIGSDLILEAEGASAEEL
                     RQPLMGALRSHFRPELINRIDEILIFEPLGRDALREIVRLELAKVERRLHDRELALEV
                     DTPVLDRLAVEGYDPVFGARPLRRVIERRIQNPLAQAILRGEFKPGERVRVSLGQDDD
                     APFRFERAEKREAVGAGR"
     gene            4524..5306
                     /locus_tag="VFR25_11085"
     CDS             4524..5306
                     /locus_tag="VFR25_11085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9941632.1"
                     /translation="MKTKRRDVRLRDRMSAPTHQPENIASVWNQVAATYASIDPEAPD
                     RIAYHGHLLQAIGDPKGLQICEVGCGSGTTSAFFASLGARITLVDLTAGALRFAHDHF
                     HELGLPADYAQMNGLRMGIRDDAFDVTWNGGVIEHFTDEGKAALLREMWRVTKPGGLL
                     LVTVPNARDYPFMAGKKIAEWRGKWIFGFEDDLTEARFRALASVAGIPGAEFRAHNPI
                     VGWWFLPYGRSITRRLGLDTIERHAKESPYGHILVMTARRPA"
     gene            complement(5325..6071)
                     /gene="ybgF"
                     /locus_tag="VFR25_11090"
     CDS             complement(5325..6071)
                     /gene="ybgF"
                     /locus_tag="VFR25_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET9941633.1"
                     /translation="MTVRNAFLALTLGSALAMAGSSCAPGGYYNTSQSALDSLLTSQA
                     ALAKRIDAMEKKLDITREGVASSRATTDTHLVEISQRLDVLDGKLEASSARVSQLSLK
                     VDTVKQKITTSDSTRASSPAGSADPETMYQAAYSDIAAGRYALGREAFQTYLKSYSDT
                     EIADNAQYWIGESYYGQGDFANAIVEFQKVIDNYPKGDKVPAAMFKIGLSQSRLKQTA
                     ESQKTYRALIQKYPKSPEAALAKERLGQKS"
     gene            complement(6068..6595)
                     /gene="pal"
                     /locus_tag="VFR25_11095"
     CDS             complement(6068..6595)
                     /gene="pal"
                     /locus_tag="VFR25_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET9941634.1"
                     /translation="MQRTPRIATLALAALLLGMLAGLGCSSRKQVTTAETTPPPPPPT
                     ETTTPPPPPSEPPSETTAEPTAAGLEDAFFDFDAAEIRNDAKDALTNNGRYLQGHGGK
                     NVVIEGHCDERGSVEYNLALGERRARAAKDFLVSYGVPAARLTTISYGKERPFDAGHD
                     ESAWAKNRRAHFISK"
     gene            complement(6675..7991)
                     /locus_tag="VFR25_11100"
     CDS             complement(6675..7991)
                     /locus_tag="VFR25_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET9941635.1"
                     /translation="MGSNTSSSRSALALLLAVALILVWPGRATTQTDVRIGVKASGTL
                     KSPLLLERFRAGAGAETDARNAHDVVRHDFDLSGVFAVADVEPLTEKLTPNPAEQGTV
                     RVQGIIEMAGPGLQFSGEVLDIGSGESVFKRSYPTTRGDIRRTMHKFNDDVIEALTGE
                     RGIAETKIAFVRTAGRVKEIWVADYDGENGRPLTHDNSIALSPAWAPWGSEIAFTTFK
                     RGNPDLYLFDLVRGASYPFSTRPGLNTAPCYSPDGKWIACTLTRDGNAEIYLVSRDAQ
                     TARRLTRNGRIDSSPSFSPTGNQIAFTSDRSGSPQVYVMDTEGSNQRLITLEGKYNDS
                     PQWSPKGDRIVYASRHDAIFDVIVMDANGQNPVQITFQAGHNENPRWSADARKIYFSS
                     TRSGQRQIYIMNPDGSDVVQLTKGGESFNPAAGPRAKKRLQAAGSG"
     gene            complement(7969..8724)
                     /locus_tag="VFR25_11105"
     CDS             complement(7969..8724)
                     /locus_tag="VFR25_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9941636.1"
                     /translation="MGWTEKPSVLRRGAAPPDQRTSAIFIAMSCLLHAALLAFFVTKA
                     SRIQDIALPASGGPSPKTRTQLVRLTPLRSFPNAEASAPAAEPERERPKPALIKRYTP
                     GAKVVVRPERGAAKKSAADETKSSPKTVAAAPQADTSPRWYSADSTKSTSVATDGDFR
                     WAYYLAAIRNKIGSRWVPPPGIEGRLVKATVYFRIHKDGQVSVASVETSSGYAFFDQT
                     AMRALLAATPLPPLPAGYTDDYLGVHFGFEYQQ"
     gene            complement(8747..9964)
                     /locus_tag="VFR25_11110"
     CDS             complement(8747..9964)
                     /locus_tag="VFR25_11110"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET9941637.1"
                     /translation="MPLLVQKYGGTSVGSPERIQKVADRIVKTRRAGTDLVVVVSAMG
                     DTTDDLLDLAAKVSDRAHPREMDMLLTAGERISMALVSMAVNDRGQEAVSFTGSQSGI
                     VTDTSHTRAKILEVKGDRIREELARGRVVIVAGFQGVSRDREVTTLGRGGSDTTAVAL
                     AAALRAESCEIYTDVDGVYTADPRLVPDARKLDQLSYDEMLELASLGAKVLHHRSVEI
                     ARRFHVPLHVRSSFHDGEGSRIGKGGSMEQVVIRGIAHDPDVAKIALLGVPDRPGVAA
                     EIFRAIGGQGVNVRMIVQASGSDGKNDVTFAVGSHEVRGVLPLIEDVRKRIGARAFVY
                     DPDVAILSVVGEGLATSPGTAGEVFAALAAEKVNIDIISTSSITITCIVRKEDLDRAV
                     KALHAALKLEQEV"
     gene            complement(9975..>10534)
                     /locus_tag="VFR25_11115"
     CDS             complement(9975..>10534)
                     /locus_tag="VFR25_11115"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET9941638.1"
                     /translation="REAIRLEGHPDNVAPALYGGFVLSMPRSDGDIEPLKLPTPRGVA
                     LTLVVPDVQVSTKEARALLPREIALKDAAANTARALALLRSLGTGRVDLLADALHDVY
                     HVPYRARLIPGYERAVEAGARAGAFGVTISGSGPTLLAFHAPGTIAGTRVGVAMIRAF
                     ARAGVHARAIPGSIAPRGAVTRTLG"
BASE COUNT         1569 a         3560 c         3596 g         1809 t
ORIGIN      
        1 gttcggcgtg ctccgatcgg tgaacacgat gtactggccg tccgccgacc agtcgggttc
       61 cgcggcgctc gagtcggcca gccactggac cgccgacgtc gcgggcgggt tcgtcgggaa
      121 cttgtacagc ccgttcgaga cctggttggc ggcacggaag aagcggaacg acatggtcac
      181 gatcgagtcc ggctttccgt ccgcgtcgat gtccggaccc caattcggca tccagaacgt
      241 gcgcgacccg aacgactggt cctcgatcgc gcgctccggg gccggaagaa cggccggcgg
      301 aaccaccgga atgtcgcggt agagcacgcg cgggacaaag gccgtggcct ggccgctatc
      361 cgagttgtac atgagccacc cgacggtgcg ggagaatctc gggttgccga agagatggga
      421 ctgtgccgtg gccgtcgtga gcacctggct cacggccggg tccggggttc cgcccgctgg
      481 ggcggagata tagacgtgct ggctgtcgtc gctcgtcggg cgcgcggcga acgcgagcac
      541 cgagccgtcg gctttcatgg tcggcgcgtt ggccgtgaag aaaccggtcg tcctctgctc
      601 cggggcgagg cccggcccgg ggatgtacca gatgtcggac tgcccgttcg tgatgttcga
      661 gtactgcgcg aagacgatgc ccccggtcgg cgccgggacc ggagccggat cgaactggac
      721 ctcctcagca tgggtccagc ggtcgtattc gttgtccacg aagatgtgga aggtggtcgt
      781 cttgaagaag tgggcgtcgc cgtcctggga ctcgatggac gccgtgacgt cgtaggtccc
      841 gaccgagtcg ggcgcctgcc agttcacggt ggccgaggtc gagtcgagga gcgtgcccga
      901 ggccgcgctc cagtggtatg tgatcgggag attgttgacg ttggtcacca ggacggtgag
      961 ctggttcgtc aagccgcgga tcgcgggctc gtggtccgcg ctgatcccaa tgatgagcgg
     1021 cgaatagacg ccgggtttgt cgtttgttcc atcaggttca ttggagcagc ccgccaggac
     1081 cagggcaagg cataccccgg ccccgaaacc tcgcaaatac ttcataaaag gggaaagctc
     1141 ctttctgggg gacgcggcgt ggtgtggcgg aaaagctacc taagaaggcg gggggccgtc
     1201 aagcccggcc cggagggctg ggtggctcta gaagatagcg cgactcggcc tccctgagaa
     1261 cccccagaaa cgcctcgggg gtggcgctcg tccggagcgc gttccggacc gactcctcct
     1321 tgaggaggcg ggagacattg gccagggcct tcacgtgggg accccggagg ctctccggcg
     1381 agaccaggag gatgaagagg gtcgccggct ctccatccat ggagtcgaac tcgatccccg
     1441 ccggcgccac cccacaggcc gccaccaggt ggtcgaggag ccgggtcttc ccgtgcggga
     1501 tggcgacgcc gttcccgatt ccggtcgaca tcatcgactc gcgctggagg accttgctca
     1561 ggatctcgcc cttggtattc acgccgtgcg cggactcgag gagcgaaacc agctccttga
     1621 tgcacgattc cttatcgcgc gccgcgaggt ggaggcggat cgcgttctcg ttgatcagct
     1681 cggacagttg catggaagcc ccgggtggcc gagggcggcg ctcgagggtg gggtgcgccg
     1741 caacgcgacg cgcgacgcta cggccggcgg catgggagcg tcaagcggaa agcgaaggga
     1801 acacggaagc tatcgctcgt cccgacccct gggcgatgcc ggctagcggc cggcgcccac
     1861 cgcttcccgc ttctccgcgc gctcgaatcg gaacggagcg tcgtcgtcct ggccgagaga
     1921 aacgcgcacg cgctccccgg gcttgaattc cccccgaaga atggcctgcg caagcggatt
     1981 ctgaatgcgg cgctcgatga cgcggcggag cggccgtgcg ccgaagaccg ggtcgtagcc
     2041 ctccacagca agccggtcga gcaccggcgt atccacttcg agcgcgagct cgcgatcgtg
     2101 aagccggcgc tccaccttgg cgagctcgag ccgcacgatc tcgcgaagcg cgtcccgtcc
     2161 gagcggctcg aagatcagga tctcgtcgat gcgattgatc agctcgggac ggaagtgcga
     2221 gcggagcgcc cccatcaagg gctggcgcag ctcctcggcg ctcgctccct cggcctcgag
     2281 gatgagatcg ctcccgatgt tgctcgtcat gatgacgagc gtgttcttga agtccacggt
     2341 gcgcccctgc ccgtcggtga gccggccgtc ctcgaagatc tggaggagga cgttcgtcac
     2401 gtcggcgtgt gccttctcga tctcgtcgag caggatgacg gcgtacggac ggcgccgcac
     2461 cgcctcggtg agctgtcctc cctcctcgta cccgacgtag cccggcggcg agccaatgag
     2521 gcgcgcgacc gtgtgcttct cctggtactc ggacatgtcg acgcgaacca tggcgcgctc
     2581 gtcgtcgaag aggaactcgg cgagcgcgcg agccagctcc gtcttgccca ccccggtggg
     2641 gccgaggaag aggaaggagc cgagaggacg gttggggtct tggagtcccg cgcgcgcgcg
     2701 gcggaccgcg tcggagacgg cgcgcaccgc ctggtcctgg ccgaccacgc gctccgtcaa
     2761 gcgctcctcc atgtggagga gcttcgcgag ctcgctctcg aggagccgct gcaccgggat
     2821 gccgctccag cgcgcgacca cttcggcgat gtcctcctcg tccacttcct ccttgaggag
     2881 cttgagatcc ttctggatca cggcgagcgc cgccgtgtct tggtcgagct gcttccgcac
     2941 cttgggcacg tctccgtggc gaatccgcgc gacgcgctcg agatctcccc cgcgctccgc
     3001 ctgctgctcc tcgtttcgga gctcctccat ccgcgcacgc ccctcgttga tcttctggac
     3061 cgccgccttc tcgcgccccc agtggtccgt gagccgcttc agttccgtct cggtctcggc
     3121 gatctcctcc tcgatgaggg cgcgtcgcgt cttcgcaccg tcatccttct ctcgcttcag
     3181 cgcgagccgc tcgatctcga gctgccgctt cttgcgctcg agcgtgtcga tctcgctggg
     3241 catgctgtcc atctcgatcc ggagcctgga tgcggcctcg tccacgagat cgatcgcctt
     3301 gtcggggagc tggcggtccg tgagatagcg gttcgacagg atggcggccg cgaccagagc
     3361 ggcgtcctgg atcttcacct tgtggaagtt ctcgtagcgc tcgcggaggc cgcggaggat
     3421 cgcgatcgtc tgctcgaccg tgggctccga cacgaaaacc ggctggaagc gccgctcgag
     3481 cgcggcgtcc ttctcgatcc gcttccggta ctcgtcgagc gtggtcgcgc cgatgcagtg
     3541 gagctcgccc cgcgcgagcg cgggcttcag gaggttcgac gcgtcgagcg ccccctcggc
     3601 cgctcccgcg cccacgagcg tgtggagctc gtcgatgaag aggatgacct gcccctcgga
     3661 gcgctgcact tccttgagca ccgacttgag ccgctcctcg aactgcccgc gatattgggc
     3721 gcccgcgagg agcgcgccca gatcgagcgc gagcacgcgc cgcgacttca gtccctccgg
     3781 cacgtccccg ctcacgatgc gctgcgcgag cccctcggcg atggccgtct tccccacgcc
     3841 cggctcgccg atcagcacgg ggttgttctt ggtgcgccgc gacagcacct ggatcacgcg
     3901 gcggatctcg tcgtctcggc cgatgaccgg atcgagcgag cccttgcgcg ccgcctcggt
     3961 caggtcccgc gcgtacttgg tgagggcctg ataggtctcc tcgggatcgg gggtcgacac
     4021 ctgctctcct ccacgcacgc tcaggagcgc gtgatagacg ttctcacggg tcgcccccgc
     4081 ttcgcgcagg atgcgcccca tctcggacgt gacggcggga tcgagcgcgc cgagcaggaa
     4141 gtgctcggtg ctcacgtact cgtcgcggag ccgctcggcc tcgacctcgg cgtgctcgag
     4201 catagacgcg acgctcggcg agaggaccgt ctgccgtccg cccgacacct tggggagcga
     4261 ttggaagcgc tgctcgagcg cgcgccggat ccggtccacc ggaacccgcg cggcctcgag
     4321 caccggcacg accgcgcccc ccttctggtc gaggagcgcc tggagcacgt gctccgtgcc
     4381 cacttcgggg tggccggcgc gctccgcgag gcgctgcgcc tcggcgagcg cttcctggct
     4441 cttggtcgtg aacttgtcga atcgcacgtc gccctcgctc ctccgttgga cgctccgcgt
     4501 ccgagtatac gcccgggcga gacttgaaga ccaagcggcg ggatgtcaga ctccgcgacc
     4561 gcatgtccgc gcccacgcac cagcccgaga acatcgcgag cgtctggaac caggtcgccg
     4621 ccacctacgc ctccatcgat cccgaggcgc cggaccgcat cgcctaccac ggccacttgc
     4681 tccaggcgat cggcgatccc aaggggctcc agatctgcga agtcggatgc gggtcgggga
     4741 cgacgagcgc attcttcgcc tcgctcggcg cgcgcatcac gctcgtggat ctgaccgcgg
     4801 gggcgctgcg attcgcgcac gaccacttcc acgagctcgg actccccgcc gactacgcgc
     4861 agatgaacgg cctccggatg gggatccggg acgacgcgtt cgacgtgacc tggaacggcg
     4921 gggtcatcga gcacttcacc gacgagggga aggcggcgct tctccgggag atgtggcgcg
     4981 tcacgaagcc gggcggactc cttctcgtga ccgtgccgaa cgcgcgcgac tatcccttca
     5041 tggcggggaa gaagatcgcg gagtggcggg ggaagtggat cttcggcttc gaggacgacc
     5101 tcaccgaggc gcgcttccgc gcgctcgcgt ccgtggccgg catcccggga gcggagttcc
     5161 gcgcgcacaa tccgatcgtg ggatggtggt tcctgcccta cggccgctcg atcacgaggc
     5221 gcctgggcct cgatacgatc gagcgtcacg cgaaggagag cccgtacggg cacatcctcg
     5281 tgatgaccgc gcgacgcccg gcgtagcgcc gcgcctgccg cgcgttacga cttctggccc
     5341 agacgctcct tcgcgagcgc ggcttcgggg ctcttcgggt acttctggat gagcgccctg
     5401 taggtcttct gtgattccgc ggtctgcttg agccgcgact gggagagccc gatcttgaac
     5461 atcgcggccg ggaccttgtc tcccttggga tagttgtcga tcaccttctg gaactcgacg
     5521 atcgcgttcg cgaagtcccc ctgcccgtag tacgactcgc cgatccagta ctgggcgttg
     5581 tcggcaattt cggtgtcgga gtagctcttg aggtaggtct ggaacgcctc ccgcccgagc
     5641 gcatagcgtc ccgccgcaat gtccgagtag gcggcctggt acatcgtctc gggatccgcc
     5701 gagccggccg gggacgatgc gcgcgtcgag tccgacgtgg tgatcttctg cttcaccgtg
     5761 tcgaccttca ggctgagctg ggagacgcgc gcgctcgagg cctcgagctt cccgtccagc
     5821 acgtcgagcc tctggctgat ctcgactaag tgcgtgtccg tggtcgcccg gctggaggcg
     5881 acgccctccc tcgtgatgtc gagcttcttc tccatcgcgt cgatccgctt cgcgagcgcg
     5941 gcctggctcg tgagcaggct gtcgagcgcc gactggctgg tgttgtagta gccgcctggg
     6001 gcgcagctcg acccggccat agcgagggcc gagccgagcg tgagcgccag gaatgcgttg
     6061 cgaaccgtca tttcgagatg aagtgcgccc tgcggttctt ggcccaggcg ctctcgtcgt
     6121 gcccggcgtc gaaggggcgc tccttgccgt aggagatcgt cgtgagacgt gccgcgggca
     6181 cgccgtagct caccaggaag tctttcgcgg cacgcgcgcg gcgctcgccg agcgcgaggt
     6241 tgtactccac cgagccgcgc tcgtcgcagt gcccttcgat gaccacgttc ttgcctccat
     6301 gcccctggag atatcgaccg ttgttggtca gggcatcctt cgcgtcattg cggatctcgg
     6361 ccgcgtcgaa gtcgaagaac gcgtcctcga gtccggccgc cgtgggctca gccgtggtct
     6421 ccgagggcgg ctccgagggc ggaggcggag gcgtggtggt ctccgtcggt ggcggcggag
     6481 gaggtgtggt ctccgcggtg gtcacctgct tgcgcgagga acatccgagg cccgccagca
     6541 tccccaaaag gagcgcggcg agggctagcg tcgcaatcct tggcgttcgc tgcatctgag
     6601 gtcctccttg attcattgtg ttggcgcgca acaacttcca caccatccca acctaccata
     6661 cgtaccccga agggtcaacc cgaaccggct gcctggaggc gcttcttggc cctgggaccg
     6721 gcggccggat tgaagctctc tccccccttc gtgagctgga cgacgtcgct cccgtccggg
     6781 ttcatgatgt agatctgccg ctgtcccgag cgcgtcgagc tgaagtagat cttccgcgcg
     6841 tcggcggacc agcgggggtt ctcgttgtga cccgcctgaa acgtgatctg gaccgggttc
     6901 tgcccgttcg cgtccatcac gatcacgtcg aagatcgcgt cgtgccgcga cgcgtacacg
     6961 atccggtccc ccttcgggga ccactgcggc gagtcgttgt acttcccttc cagcgtgatg
     7021 agcctctggt tcgagccctc cgtgtccatg acgtagacct gcggcgatcc ggagcggtcg
     7081 gacgtgaacg cgatctggtt ccccgtcggg ctgaaggaag gcgacgaatc gatccggccg
     7141 ttcctcgtga ggcgccgcgc cgtctgcgcg tcgcggctca ccagatagat ctcggcattg
     7201 ccgtcgcgcg tcagcgtgca cgcgatccac ttcccgtcgg gggagtagca gggagccgtg
     7261 ttgagcccgg ggcgcgtcga aaagggatac gaggcgccgc gcacgagatc gaacagatag
     7321 agatcggggt tcccgcgctt gaacgtggtg aaggcgatct ccgagcccca cggcgcccag
     7381 gccggcgaga gcgcgatcga gttgtcgtgc gtgagcgggc gtccgttctc tccgtcgtag
     7441 tcggcgaccc agatctcctt cacccgtccc gccgtgcgca cgaacgcgat cttggtctcc
     7501 gcgatgcccc gctccccggt cagggcctcg atcacgtcgt cgttgaactt gtgcatcgta
     7561 cgtcggatgt cgccgcgcgt ggtcggatac gaccgcttga agaccgactc cccacttccg
     7621 atatcgagca cttcccccga aaactgaagc cctggtcccg ccatttcgat aattccttgc
     7681 acgcggaccg ttccctgctc cgcgggattc ggcgtcagct tctccgtgag cggctcgacg
     7741 tcggccaccg cgaagactcc ggagagatcg aagtcatgtc gcacgacgtc gtgcgcattg
     7801 cgagcgtcgg tctccgcccc cgcgcccgcg cggaaacgct cgaggaggag cggcgacttg
     7861 agggtgccgg acgccttcac gccgatgcgc acgtcggtct gcgtcgtggc ccgtcccggc
     7921 cagacgagga tgagcgctac ggccaggaga agcgcgagcg cggagcggct actgctggta
     7981 ttcgaaccca aagtggaccc ccaggtagtc gtccgtgtat cccgcgggca gcggcgggag
     8041 cggtgtggcg gcgagcagcg cccgcatcgc cgtctgatcg aaaaaagcgt atcccgaaga
     8101 agtctcgacg gaagcgacgg aaacctggcc gtctttgtga atgcggaagt agaccgtggc
     8161 tttgaccagc cgcccctcga tccctggggg aggtacccag cgcgagccga tcttgttccg
     8221 gatcgcggcg aggtaatagg cccagcggaa atcgccgtcg gtcgcgacgc tcgtgctctt
     8281 cgtcgaatcg gcggagtacc agcgcgggct cgtgtccgcc tgcggcgcgg ccgccacggt
     8341 cttcggcgaa gacttggtct cgtccgcggc gctcttcttc gccgcgcccc gctcggggcg
     8401 caccacgacc ttcgcgcccg gcgtgtatcg cttgatgagt gcgggcttgg ggcgctcgcg
     8461 ctccggctcc gccgccggcg cggaggcttc cgcgttgggg aaggagcgga gcggggtcag
     8521 acggacgagc tgcgtgcggg tcttcgggga tggaccgccc gatgccggaa gcgcgatgtc
     8581 ctggatccgg ctcgccttcg tcacgaagaa cgcgagcagg gcggcatgaa ggaggcacga
     8641 catcgcgatg aagatcgctg acgtgcgctg atccggcggg gccgcgccgc gccggagcac
     8701 cgaaggcttc tcggtccacc ccatggggga tctcccatcc ttggtcctag acttcctgct
     8761 ccagcttgag cgcggcgtgg agcgccttca cagcgcggtc caggtcttcc ttccgcacga
     8821 tgcaggtgat cgtgatcgag gacgtgctga tgatgtcgat gttcaccttc tccgcggcga
     8881 gggccgcgaa gacctcgccg gccgtgccgg gcgacgtggc gagcccttcc ccgaccacgg
     8941 agaggatggc cacgtccggg tcgtagacga acgcgcgcgc gccgatccgc ttccgcacgt
     9001 cctcgatcag gggcagaacg ccccgcacct catgagatcc tacggcgaag gtgacgtcgt
     9061 tcttcccgtc cgatccgctc gcctggacga tcatccggac gttcaccccc tgtccgccga
     9121 tcgcgcggaa gatctccgcc gccacgcccg gccggtcggg cacgcccagg agcgcgatct
     9181 tcgccacgtc cggatcgtgg gcgatgcccc ggatcaccac ctgttccatc gagccaccct
     9241 ttccgattct ggatccttcg ccgtcgtgaa agctcgatcg cacgtggagc ggcacgtgga
     9301 agcgccgcgc gatctcgacc gagcgatgat ggagaacctt ggcgccgagc gaagccagct
     9361 cgagcatctc atcgtacgag agctggtcca gcttgcgcgc atcgggtacg aggcgcgggt
     9421 ccgcggtgta gacaccgtcc acgtcggtgt agatctcgca ggactccgcc ctgagcgcgg
     9481 cggccagggc caccgccgtc gtgtccgagc ccccccgtcc gagggtggtc acctcccgat
     9541 ccctgctcac cccttggaac cccgcgacga tcacgactcg cccgcgggcg agctcttccc
     9601 ggatgcggtc tcccttgacc tcgaggatct tggcccgcgt gtgagaggtg tcggtgacga
     9661 tgccgctctg ggagccggtg aaggagaccg cctcctgccc ccggtcgttc accgccatgg
     9721 agacgagagc catcgagatc cgctcccccg cggtgaggag catgtccatc tcgcgcggat
     9781 gcgcccggtc ggagaccttg gcggcgagat cgaggaggtc gtcggtcgtg tctcccatcg
     9841 cggagacgac gacgacgaga tcggtgccgg cgcggcgggt cttgacgata cggtccgcga
     9901 ccttctggat gcgctcgggc gaaccgaccg aggttccacc gtatttctgg acgagaagcg
     9961 gcagcgacgg ccccctagcc gagggtgcgg gtcaccgcgc cgcgcggcgc gatggacccc
    10021 ggaatcgccc gcgcgtgcac tccggcacgc gcgaaggcgc ggatcatggc cacgcccacc
    10081 cgcgtccccg cgatcgtccc cggggcgtga aacgcgagca gtgtgggacc gcttccgctg
    10141 atggtgaccc cgaacgcgcc tgcgcgcgcc cccgcttcca ccgcgcgctc atagcccggg
    10201 atgagccgag cgcgataagg cacatggtac acgtcatgga gcgcgtcggc caagagatcc
    10261 acgcgtcccg tcccgagcga gcggaggagc gccagcgcgc gcgcggtgtt cgccgccgcg
    10321 tccttgagcg cgatctcgcg cgggaggagc gcgcgcgctt ccttcgtgct cacctggacg
    10381 tcgggcacga cgagcgtgag cgcgacgccg cgcggggtcg ggagcttgag cggctcgatg
    10441 tcgccgtcgg agcgcggcat cgagagcacg aagcctccgt agagcgcggg agccacgttg
    10501 tcgggatgcc cctccaggcg aatggcctcg cgga
//
LOCUS       DASTKC010000265         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1178511, whole genome shotgun sequence.
ACCESSION   DASTKC010000265 DASTKC010000000
VERSION     DASTKC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178511"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFR25_11120"
     CDS             <1..222
                     /locus_tag="VFR25_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941639.1"
                     /translation="SEAEILYKTLKALRKDVELVLFPGESHGLSRGGTPSRRLARLHI
                     MRDWFHRHLVDGSTKKAEANSEAAMAAVR"
     gene            232..1026
                     /locus_tag="VFR25_11125"
     CDS             232..1026
                     /locus_tag="VFR25_11125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9941640.1"
                     /translation="MELDNRAILLTGASGGIGRATALRLARLGARVGVMARGESEVRA
                     LAEEIVTAGGEAVAIQGDVRSQSDAARAVAAMVDRFGGLDALVNNAGLGYLRAVEEAT
                     DAEIEQMVETNLLGVIRMTRAALPKLLARPGSALVNVASYAGKVGAPYYSFYAATKFA
                     VVGLTEAWRRELGPRGLKVTLLLPAAVETSFLEKAGRDRALGLGPAGTILRPEQVAAG
                     IERALRSHPPQIYLPARNRALAMLNSALPGLSDRIVNALFRYPRGR"
     gene            1023..2222
                     /locus_tag="VFR25_11130"
     CDS             1023..2222
                     /locus_tag="VFR25_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9941641.1"
                     /translation="MITPEPEANRPPARSDSSKAAWPSFRSRGDNTPSTGRRKICLSL
                     SGGGLSGAFYEIGCLAALDDFLGFDFSSTGFDIYIGVSAGASVAAVLAKGIPARKVYW
                     GLLTDADPALQFRRGDMYDVNRWAILRSFLKITWSIPGYFVRRVKGGQRPSPFDFMHY
                     IEERLPAGLFALDNFQDFFTSVLEAHGKSGDFADLEKELYIPATDLDLGTRRIFSNVE
                     GSTCKIGDVVAASSAIPLLFRPVRIEGRDYIDGSTGKVAHIDVAVRAGASTILIINPL
                     VNISNDRQTVCLPRGDGRCATIREKGMSFIQDQASRIETRVRLDGEIRRCQVEYPSVK
                     LYRIEPSSSEPMMFLHHTMSYTEREAILRYGYTSMTAILRRRRAVIEELMQFHGLNPP
                     DEISVGG"
     gene            2253..>2996
                     /locus_tag="VFR25_11135"
     CDS             2253..>2996
                     /locus_tag="VFR25_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9941642.1"
                     /translation="MALDPIQARLTRGDIVLLDGAMGTELERLGVPTTLPLWTAQALL
                     DAPDRVREVHEGYILAGADVITAATFRTTPRSLKKAGLEAEADRLTALGVALAKEARA
                     HVAAGREVRVAGSMAPLEDCYRPERSPAAPDAEREHRDQAERLARAGAELLLIETMGT
                     AAEAAAAVRAARTTELPVWISFLCKSGTELYSGEPLAAAVRLVTTLAPDAVLVNCVPV
                     AIATECMEVLGRSTALPYGCYANAGSPDLG"
BASE COUNT          455 a         1069 c         1013 g          459 t
ORIGIN      
        1 tcggaggcgg agatcctcta caagaccctc aaggcgctcc ggaaggacgt ggagctcgtc
       61 ctcttccccg gcgagagcca cggcctgtcg cgcgggggca caccgtcccg tcggctggcc
      121 cgcctccaca tcatgcgcga ctggttccac cgccacctgg tcgacggctc cacgaagaag
      181 gccgaggcca attccgaagc cgccatggcg gcggtgcgct gagcgaggtg agtggagctc
      241 gataaccgcg ccatcctcct gacaggcgcg tcgggcggga tcggccgcgc caccgcgctt
      301 cggctcgcgc gcttaggcgc gcgcgtcggc gtgatggcgc gcggcgagtc cgaggttcgc
      361 gcactcgccg aggagatcgt gacggcagga ggcgaggccg tcgcgatcca gggggacgtg
      421 cgctcccaat ccgacgcggc gcgcgccgtg gccgccatgg tcgaccgctt cggcggcctc
      481 gacgccctcg tgaacaacgc ggggctcggc taccttcgcg cggtggaaga ggcgacggac
      541 gccgagatcg agcagatggt ggagaccaac ctcctcggcg tgatccggat gacccgggcc
      601 gcgcttccca agctcctcgc ccgccccggg agtgccctcg tgaacgtcgc gagctacgcg
      661 gggaaggtgg gcgcccccta ctactcgttc tacgcggcca ccaagttcgc ggtcgtcggg
      721 ctgaccgagg cctggcggcg cgagctcggc ccgcgcggtc tcaaggtcac gctgctcctg
      781 cccgccgcgg tcgagacctc gttcctcgag aaggcaggac gcgatcgggc actcggtctg
      841 ggcccggcgg gcaccatcct tcgcccggag caggtcgcgg ccggcatcga acgcgcgctc
      901 cgaagccatc cgccgcaaat ctaccttccg gcccggaacc gggcgcttgc gatgctgaat
      961 tccgccctgc cgggcctctc cgatcgcatc gtgaacgccc ttttccgtta tcctagaggt
     1021 cgatgatcac acccgagccc gaagcgaacc ggccgccggc caggagcgac tcctccaagg
     1081 ccgcgtggcc gtcgttccgc tcccgaggcg acaacacccc atccaccggg cgccgcaaga
     1141 tctgcctgag cctttccggc ggcggtctct ccggcgcgtt ctacgagatc ggctgcctcg
     1201 ccgcgctcga cgacttcctc ggcttcgact tctcgagcac gggcttcgac atctacatcg
     1261 gcgtgagcgc cggcgcctcg gtggccgcgg tgctcgcgaa gggaatcccc gcgcggaagg
     1321 tctactgggg cctcctcacc gacgccgatc ccgcgctcca gttccgccgc ggcgacatgt
     1381 acgacgtgaa ccgctgggcg atcctccgct cgttcctcaa gatcacctgg tccatccccg
     1441 gctacttcgt gcggcgggtg aagggcgggc agcgtccatc gccgttcgac ttcatgcact
     1501 acatcgagga gcgcctcccg gccgggctct tcgcgctcga caacttccag gacttcttca
     1561 cgagcgtcct cgaagcgcac ggcaagtcgg gggacttcgc ggatctcgag aaggagctct
     1621 acatcccggc caccgatctc gatctcggga cgcggcgcat cttctcgaat gtcgagggat
     1681 cgacgtgcaa gatcggggac gtggtcgcgg catcgtcggc cattcccttg ctcttccggc
     1741 cggtgcggat cgaggggcgc gactacatcg acggctcgac ggggaaggtg gcgcacatcg
     1801 acgtcgcggt gcgcgcgggg gcgtcgacga tcctcatcat caatcctctg gtgaacatct
     1861 ccaatgatcg ccagacggtc tgcctgccgc gcggcgacgg acgatgcgcc acgattcgcg
     1921 agaaggggat gtctttcatc caggaccagg cctcgcgcat cgagacgcgg gtgcgtctgg
     1981 acggagagat ccgccgctgc caggtcgagt acccgagcgt gaagctctac cgcatcgagc
     2041 cctcgtccag cgagccgatg atgttcctcc accacaccat gagctatacc gagcgggagg
     2101 cgatcctccg ctacggctac acctcgatga ccgcgatcct gcgccgccgg cgcgcggtga
     2161 tcgaggagct gatgcagttc cacggactca atcccccgga cgagatctcg gtcggaggct
     2221 gacgcctccg gctcccacga gaggttgcac ggatggccct cgatcccatt caagcccggc
     2281 tgacacgggg cgacatcgtc ctcctcgacg gagcgatggg cacggagctc gagcgtctgg
     2341 gtgtcccgac cacgctccca ctctggaccg cgcaggcgct cctcgacgcg cccgatcgcg
     2401 tgcgcgaggt gcacgagggc tacatcctgg ccggcgccga cgtgatcacc gccgcgacgt
     2461 tccgcacgac gccgcgctcg ttgaagaagg cgggactcga ggcggaggcg gaccggttga
     2521 cggcgctggg tgtggcgctc gcgaaggaag cgcgcgctca cgtcgccgcg ggcagggagg
     2581 ttcgggtggc cggatcgatg gcgcccttgg aggactgcta ccggcccgag cgatcgcccg
     2641 ccgcgccgga cgcggagcgc gagcatcgcg accaggcgga gcgcctcgcg cgcgcgggcg
     2701 cggagctctt gctgatcgag acgatgggaa ccgccgccga agcggccgcg gccgtgcgcg
     2761 ccgcacgcac gacggagctt cccgtgtgga tcagcttcct ctgcaagagc gggacggagc
     2821 tctatagcgg cgaaccgctc gccgcggccg tgcgcctggt gacgaccctt gcgcccgacg
     2881 cggtgctcgt caactgcgtg cccgtcgcga tcgcgaccga gtgcatggag gttttaggac
     2941 gctccaccgc gctcccgtac ggctgctacg ccaacgccgg ctctcccgac ctcgga
//
LOCUS       DASTKC010000266         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_1414127, whole genome shotgun sequence.
ACCESSION   DASTKC010000266 DASTKC010000000
VERSION     DASTKC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414127"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..521)
                     /locus_tag="VFR25_11140"
     CDS             complement(6..521)
                     /locus_tag="VFR25_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9941643.1"
                     /translation="MKDDRHHTARPSAPTIDGPQKPPRPPIGLPLLPDGRTALPKGNP
                     CQGCDHCCRYIAIQIDTPRTKKNFEDIRWYVLHRGVSVWVDWDGYWMLQFETQCDWLK
                     DGRCSHYALRPDLCREYDPAECERYSHTPAEKHLFRNEQDLDAYLAKREERLQKRRAR
                     TNGRNGKAKAS"
     gene            538..>2799
                     /locus_tag="VFR25_11145"
     CDS             538..>2799
                     /locus_tag="VFR25_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941644.1"
                     /translation="MGSSGDAFAQHSAIGRKLSHYLIERHLGAGGMGEVYLAQDVALG
                     RAVALKILRTGDSSFGERLRREAEAFARLQHPWIATFYEASEEDGVPFLAMEYVPGRT
                     LRQILGGGAMPVDRALALAGCLLEALAHAHAAGVLHRDIKPENVMVLDGEGAKLLDFG
                     LAKYSMAEPATDKTQTRLTTQGEVLGTMGYMAPEQLRGLEIDARADLYAVGAVLHECI
                     AGRPAFPGASATERMAATLAGDRVPLRGPTIPPELEALVARALARSPGDRFPSASAFL
                     SDVRRLRGDSSASALPDTLAVLDLENVTKRPEDDWMTSGIAESLASDLARVPGLHVVS
                     REKLLQARGALAQGGAAVDALDLGHRLACRWVLSGGIQRMGPSLRVVARLTEVPTGRM
                     VASEKVDGTVDGIFEVQDRLAQAVTAQLNLVAPSPIPDAGGSPDVRAFELTARGRRLT
                     NQLEKGTFQEAQELFEEAIRIDPKNALALNGLAEIWSLRFPFTSDPAMLDESERYARR
                     AIEADPKLGSPHVWLGYVALRRNDAPASFDAMQRALELEPGLPEAFYFAACAKAMMRR
                     NEEAIPYYRDAVATGRVYWFAWLGLGWAYCELDQMDEALWCMGKAIEGERSGASGATP
                     GAGGHAAECLRRRGDLDAARRSALEGLASSEQTDHMYRDTFRGVCLVSLGRIALRQGD
                     LDAARTAFQQAVLLVRGRPKALGAGHLLVQALSGLAQSGSGAALLDEALALETSRSDF
                     NFQWLWCCQPEISH"
BASE COUNT          389 a          961 c          989 g          460 t
ORIGIN      
        1 ctgagctagc tcgccttcgc cttcccgttc cgcccgttcg tccgcgcgcg gcgcttctgc
       61 aggcgctcct cgcgcttggc cagataggcg tcgagatcct gctcgttccg gaacagatgc
      121 ttctcggcgg gggtgtggct gtagcgctcg cactcggcag ggtcgtactc gcggcagaga
      181 tccggacgca gcgcgtagtg gctgcagcgg ccgtccttga gccagtcgca ctgggtctcg
      241 aactggagca tccagtagcc gtcccagtcc acccacaccg agacgccgcg gtggagcacg
      301 taccagcgga tgtcctcgaa gttcttcttt gtgcgcggcg tgtcgatctg gatcgcgatg
      361 tagcggcagc aatggtcgca tccctggcag ggattcccct tcggaagcgc ggtccgtccg
      421 tccgggagaa gcggaagccc gatcggcgga cgcggcggct tctgggggcc gtcgatggtc
      481 ggcgcgctgg ggcgtgcggt atggtgtctg tcgtccttca cgtggaggcc tcccgtcatg
      541 gggtcgtcgg gcgatgcgtt cgcgcagcat agcgcgatcg gtcgcaagct gtctcactat
      601 ctgatcgagc gccacctggg tgcgggcgga atgggggagg tttacctggc ccaggacgtc
      661 gcgctgggcc gcgccgtcgc gctcaagatc ctccgcaccg gcgactcctc cttcggcgag
      721 cgcctcaggc gcgaggcgga ggccttcgcg cgcctccagc acccctggat cgccaccttc
      781 tacgaggcga gcgaggagga cggggttccc ttcctcgcca tggagtacgt gccggggcgc
      841 acgctccgcc agatcctcgg tggtggggcg atgcctgtcg accgcgcgct cgccctcgcg
      901 gggtgcctgc tcgaagcgct cgcccacgcg cacgcggccg gcgtgctcca ccgcgacatc
      961 aagcccgaga acgtcatggt gctcgacggc gaaggggcga agctcctcga cttcgggctc
     1021 gccaagtact ccatggccga gccggccacc gacaagaccc agactcgcct tacgacccag
     1081 ggggaggtgc tgggcacgat gggctacatg gctccggagc agctccgcgg gctcgagatc
     1141 gacgcgcggg cggatctcta tgccgtaggg gccgtgctcc acgagtgcat cgcggggcgt
     1201 cccgcgtttc cgggcgcctc ggcgaccgaa cgaatggccg cgaccctcgc gggggatcgc
     1261 gttccgctcc gcggcccgac gatccccccg gagctcgagg cgctggtggc gcgcgctctc
     1321 gcccgctctc ccggggatcg ctttccatcg gcctccgcgt tcctctccga tgtgaggcgc
     1381 ctccgcggcg actcgtcggc aagtgccctg ccggacacgc tcgcggtgct cgatctggag
     1441 aacgtgacga agcgtcccga ggacgactgg atgacgagcg gcatcgcgga aagcctcgcc
     1501 tcggatctcg cccgcgtccc gggcctccac gtcgtgtcgc gcgagaagct gctccaggcg
     1561 cgaggggcgc tcgcccaggg cggcgccgcg gtcgacgcgc tcgatttagg gcatcgactc
     1621 gcttgccggt gggtgctctc cggcggcatc cagcgcatgg gcccgtcgct ccgcgtggtc
     1681 gcgcgcctga ccgaggtgcc gacggggcgg atggtcgcct ccgagaaggt ggacggcacc
     1741 gtcgacggaa tcttcgaagt ccaggaccgg ctggctcaag ccgtgaccgc gcagctcaac
     1801 ctcgtcgcgc catcgccgat tcccgacgcc ggaggctcgc cggatgtgcg cgcgttcgag
     1861 ctgaccgcgc gcggacgccg gctcaccaac cagctcgaga aggggacgtt ccaggaggcg
     1921 caggagctat tcgaggaggc cattcgaatc gatccgaaga acgctctcgc gctcaacggg
     1981 ctcgcggaga tctggtcgct ccgctttccg ttcaccagcg atcccgccat gctcgacgaa
     2041 tcggagcggt acgcacgtcg ggccatcgag gcggatccga agctgggcag tccccatgtc
     2101 tggctgggct acgtggcgct gcgccggaac gacgcgccgg cttccttcga cgcgatgcag
     2161 cgcgcgctcg agctcgagcc gggattgccc gaagcgttct acttcgcggc ttgcgccaaa
     2221 gcgatgatgc gccgaaacga ggaggcgatc ccctactacc gcgacgctgt cgcgaccggt
     2281 cgcgtctact ggttcgcgtg gctcgggctc ggatgggcgt actgcgagct cgatcagatg
     2341 gacgaagccc tgtggtgcat ggggaaggcg atcgaaggcg agcgcagcgg tgcgtcgggc
     2401 gccaccccgg gagccggagg acacgcagcc gagtgtctca ggcggcgcgg cgatctcgac
     2461 gcggcgcgcc gaagcgcgct cgaggggctc gcgtcgtccg aacagaccga ccacatgtac
     2521 cgcgacacgt ttcgcggcgt ctgtctcgtg agcctcggcc gcattgcgct gcgccagggc
     2581 gatctcgatg cggcgcgcac ggcgttccag caagccgtgc ttctcgtccg cggacgtccc
     2641 aaggcgctcg gagcggggca cctgctcgtg caggcgctgt ccgggttggc tcagtcgggg
     2701 tccggggccg cccttctgga cgaggccctc gctctggaga cgagccggtc ggacttcaat
     2761 ttccagtggc tctggtgctg ccagcccgag atctcccac
//
LOCUS       DASTKC010000267         4628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_941777, whole genome shotgun sequence.
ACCESSION   DASTKC010000267 DASTKC010000000
VERSION     DASTKC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941777"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VFR25_11150"
     CDS             complement(<1..1845)
                     /locus_tag="VFR25_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9941645.1"
                     /translation="MRSCKTRRHVAARAVRPTSISSFLALVLVAFAVPAFAQTGSLVV
                     KVTDQGGAPLQQASVFVLGTSTGGPTNSQGIATVIHIPVGTYTVRVKMIGMAQQERMG
                     VKIDANRSTSLEFKLVEQAVQMKTIDVRGDQKIAIRKKDSSTKQIVTSEDLHSLPVDS
                     YKDAIGLKAGVISQNGELHFRGGRGDEVLTVVNGIASRNPLRAEGVDLGLLAVSSSEQ
                     VLGGLDAQYGNALSGVISLTTREGGDKFTGEARYFTDRYGEHDKEFNNFERISLGFGG
                     PFLFNKTNYYISYEGTWTDTYLANTSEHNEHRFLDFIRTGLRQANASNLSSKLTYKVT
                     PNAKLNFEFIDNHTLDGRYHNRWNREGFVQVIQDSTAPTDGTITRRYGTWSYYPVDSS
                     YVPMNTADHLPVTDASYQQVALTWRHTLGIGEIYNLRASRQQWKSTEDVLDRQLWEYQ
                     QRPNNYYDPLNRLDGQYYVTNGDYPFYERRNTATYTLNGDYSKKVKAHNFMVGGDINY
                     NDLSFLLTQFPNVLDANGDYGATRDEFQNYNPEGSFFAQDRWEYEGMVLNAGVRYDSF
                     SVGNQISSAEVADKVKTQWSPRVGIAYPISDRDVMSFHYGRLFQVPDRL"
     gene            complement(2127..3473)
                     /locus_tag="VFR25_11155"
     CDS             complement(2127..3473)
                     /locus_tag="VFR25_11155"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET9941646.1"
                     /translation="MTEKEARALLDQVLGAAQGREVEAILGGGVSRLTRFANNEITQN
                     VSENRVELSVRVVDGKRTGRANGNDLSQQGIERLLDAAAAAARVQPEIPDLLPLPGRQ
                     SYLTVDADDSTTGDLDADGRASEVKRAVERCVEAGVTAAGIYETGRGTIGDYGMLDAM
                     AIANTSGLFAYHRGTHATFRISALDGAASGWAERESWRASDVDGAALADRAVEKAIRS
                     RGAMPWDPGAYTVVLEPAAVADLLMDLSWTSLGALAMQEGRSFLSGRIGERVMGEKIT
                     LRDDPFHPLHRGSPFDAEGIPTRPTTLIERGIARGPVYDRPTAAKEGKESTGHSLPIP
                     NTIGPLARHLVLDGGAGPPEALLDGIERGLWVTRVWYTNVVDPMTATLTGMTRDGLFA
                     IENGRITHAVRNFRFNQSVVEMLNRVEGMSESVRAGGVVCPALRIQGFHMSSVTEF"
     gene            complement(3466..>4628)
                     /locus_tag="VFR25_11160"
     CDS             complement(3466..>4628)
                     /locus_tag="VFR25_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9941647.1"
                     /translation="LAAEPPHRAVWTSPCVIDPFDVPLERKLDLLFRIDEALRRVPGV
                     AIAEGFLMFVKKRQIYVSTEGSDIDQTIVRSGGGYSATAVSATEVQRRSFPQAEGFHQ
                     TRGYELIESIPWVENAPRVGEEAVALLTADPCPQGERDIILEGSQLALQIHESVGHPT
                     ELDRVLGMEANYAGTSFLTTDQLRVLRFGSEHVTLMADGTLPGGLATFGYDDEGVEAQ
                     RWPIVENGLFVGYLTSRELAHTIGESRSRGCVRADGWHHVPMIRMVNLSLMPGRGSLD
                     DLLADTDDGLYLETNRSWSIDQLRYNFQFKTEIAWEIKKGRRVRMLRNPTYQGITPRF
                     WNSCDFVCGPEAWKPWGVTNCGKGQPGQVAEMTHGSAPARFRKVNVGVGYDD"
BASE COUNT          741 a         1484 c         1536 g          867 t
ORIGIN      
        1 caggcgatcc ggcacctgga agagacggcc gtagtggaag ctcatgacgt cgcggtcgga
       61 aatcggatac gcgatgccga cgcgcgggct ccactgcgtc ttcaccttgt ccgcgacctc
      121 ggccgaggag atctggttcc ccaccgagaa gctgtcgtac cggacgcccg cgttcaggac
      181 catgccctcg tactcccagc ggtcctgggc gaagaaggaa ccctcggggt tgtagttctg
      241 gaactcgtcc cgcgtggcgc catagtcccc gttcgcgtcg agcacgttcg ggaactgcgt
      301 gaggaggaac gagaggtcgt tgtagttgat gtcgccgccg accatgaagt tgtgggcctt
      361 gaccttcttc gagtagtcgc cgttcagcgt gtaggtggcc gtgttccggc gctcgtagaa
      421 ggggtagtcc ccgttcgtca cgtagtactg gccgtccagc cggttgagcg ggtcgtagta
      481 attgttcggc cgctgctggt actcccagag ctggcgatcg agcacgtctt ccgtggactt
      541 ccactgctgc cgcgaggcgc ggaggttgta gatctccccg atcccgagcg tgtgcctcca
      601 ggtgagcgcc acctgctggt acgacgcgtc cgtcaccggc agatggtcgg ccgtgttcat
      661 cgggacgtac gacgagtcga cgggatagta ggaccaggtc ccgtagcggc gggtgatcgt
      721 gccgtccgtg ggcgccgtcg aatcctggat gacctggacg aagccttccc ggttccagcg
      781 attgtggtac cggccatcca gtgtgtggtt gtcgatgaac tcgaagttca acttcgcgtt
      841 cggggtgacc ttgtaggtca gcttgctcga gaggttgctg gcgttggcct ggcggagtcc
      901 cgtccggatg aagtcgagga accggtgctc gttgtgctcg gaggtattgg ccaggtaggt
      961 gtcggtccag gtgccctcgt acgagatgta gtagttcgtc ttgttgaaga ggaacggtcc
     1021 gccgaaaccg agcgagatgc gctcgaagtt gttgaactct ttgtcgtgct cgccgtagcg
     1081 atccgtgaag tagcgcgcct cgcccgtgaa cttgtcgccg ccctcgcgcg tggtcaggct
     1141 gatgacgccg gagagcgcgt tcccgtactg ggcgtcgagt ccgccgagca cctgctcgct
     1201 cgaggacacc gcgaggagtc cgaggtccac gccctcggcg cgaagcggat tgcggctcgc
     1261 gatgccgttc acgaccgtga gcacttcatc cccacggccg cctcggaagt ggagctcccc
     1321 gttctggctg atgacgcccg ccttcaatcc gatcgcgtcc ttgtagctgt cgacagggag
     1381 cgagtgcagg tcttcgctcg tgacgatctg cttggtggag ctgtccttct tacggatggc
     1441 gattttctga tcgccgcgga cgtcgatcgt cttcatctgg acggcctgct cgaccagctt
     1501 gaattccaga gacgtactgc ggttcgcgtc gatcttgacg cccatgcgct cctgctgcgc
     1561 catgccgatc atcttcacgc gaacggtata ggtcccgacg ggaatatgga tcaccgtcgc
     1621 gatgccttga gagttggtcg gaccgcccgt gctcgttccc aggacgaaga cactcgcctg
     1681 ctgtagtggc gctcccccct ggtccgtcac cttcacgacg agagagcccg tttgcgcgaa
     1741 ggcggggacc gcgaacgcta cgaggacgag tgcgagaaac gaggagatcg aggtcggccg
     1801 caccgctcgg gcggctacgt gacgccgcgt cttgcagctc ctcatggatt cgtccactcc
     1861 tttcgacgac aagattcgaa gggtcgagaa agcgtaatgt ttatgcggtt tttcgcgccc
     1921 cgagtcaagg caattcattg cagagcacct gcttggagcc gtcgaacgat gttacggaag
     1981 agcacgtcgc acatgccatg ggccaaatcc ggacgggatg gtcggggggc attcgacgtc
     2041 agggggggga acttcaggag acgtaccgaa gtacgttctc ttgtgtggga gtatatccgg
     2101 aaagtctcgg tcgacaaggg attttttcaa aattccgtca cactggacat gtggaagccc
     2161 tgaattcgta gcgccgggca gaccacgccg cccgcccgca ccgactccga catcccctcc
     2221 acccggttca gcatctcgac gacgctctga ttgaagcgga agttacggac cgcgtgggtg
     2281 atgcgcccgt tctcgatcgc gaagaggccg tcccgggtca tccccgtgag ggtcgccgtc
     2341 atgggatcga cgacgttcgt gtaccacacc cgcgtgaccc acagcccccg ctcgatcccg
     2401 tcgagcaggg cctcgggggg accggctcct ccgtccagaa ccagatggcg cgcgagcggg
     2461 ccgatcgtgt tgggaatggg gaggctatgc ccggtcgact ccttcccttc cttcgccgcc
     2521 gtggggcggt cgtagacggg cccccgggcg atcccgcgct cgatcaaggt ggtgggccgg
     2581 gtgggaattc cctcggcgtc gaacggagag ccgcgatgga ggggatggaa ggggtcgtcc
     2641 cggagcgtga tcttctcccc catcacgcgc tccccaatcc tgcctgagag gaagctccgc
     2701 ccctcctgca tggccagggc tccgagggag gtccaggaga gatccatgag gagatccgcg
     2761 acggccgcag gctcgaggac cacggtgtac gcgccggggt cccagggcat agcgccgcgg
     2821 gagcggatcg ccttctccac cgcccgatcg gcgagcgcgg cgccgtccac gtcggaggca
     2881 cgccagctct cgcgctccgc ccagcccgac gcggcgccgt cgagcgctga gatgcgaaag
     2941 gtggcgtgcg tgccgcgatg gtaggcaaag agcccgctcg tgttcgcgat ggccatcgcg
     3001 tcgagcatgc cgtaatcgcc gatcgtgccc cggcctgtct cgtagatgcc ggccgcggtc
     3061 acgccggcct cgacgcatcg ctcgaccgcg cgtttgacct cgctcgcgcg tccgtccgcg
     3121 tcgaggtctc ccgtggtcga atcgtccgcg tcgacggtca gatacgactg ccgtcccggg
     3181 agcggcagga gatccggtat ctccggctgg acacgagccg cggccgcggc cgcatcgagg
     3241 agccgctcga tgccctgctg ggatagatcg ttcccgttcg cccggccggt ccgcttccca
     3301 tccaccacgc ggacggagag ctcgacccgg ttttcggaga cgttctgcgt gatctcgttg
     3361 ttcgcgaatc gcgtgagccg gctcacgccg ccgcccagga tcgcctcgac ctcgcgtccc
     3421 tgagccgcgc cgaggacctg gtcgagaaga gcccgcgcct ccttctcagt catcgtatcc
     3481 gaccccgacg ttcaccttgc ggaagcgggc cggtgcggag ccgtgggtca tctcggccac
     3541 ctgcccgggc tgccccttgc cgcagttggt cacgccccac ggcttccatg cctcggggcc
     3601 gcagacgaag tcgcaggaat tccagaatcg cggcgtgatt ccctggtagg tgggattgcg
     3661 cagcatccgc acgcgcctcc ccttcttgat ctcccacgcg atctcggtct tgaactggaa
     3721 gttgtaccgg agctggtcga tgctccagct gcgattggtt tcgaggtaga gaccgtcatc
     3781 ggtgtcggcg aggaggtcgt cgagcgagcc ccgccccggc atgaggctca aattcaccat
     3841 gcggatcatg gggacgtggt gccagccgtc cgcccggacg cagccgcggc tccgcgattc
     3901 gccgatcgtg tgcgccagct cgcgcgaggt gagatagccg acgaacaagc cgttctcgac
     3961 gatcggccag cgctgtgctt ccaccccctc gtcgtcgtag ccgaaggtgg cgaggccgcc
     4021 tggaagcgtg ccgtcggcca tcaaggtgac gtgctccgat ccgaagcgga gcacgcggag
     4081 ctggtcggtc gtcaggaagc tcgtgcccgc gtagttcgcc tccatcccga gcacgcggtc
     4141 gagctcggtt ggatgcccca cggactcgtg gatctgaagc gcgagctgcg atccttcgag
     4201 gatgatgtcg cgctcccctt gcggacaggg atcggccgtc aagagggcca cggcttcctc
     4261 gccgacccgc ggcgcgttct ccacccaggg gatcgactcg atcaactcgt agccccgcgt
     4321 ctgatgaaat ccctcggcct gagggaagct ccggcgctgg acttcggtgg cggagaccgc
     4381 cgtcgcgctg tagccgccgc ccgaccgaac gatcgtctga tcgatgtcgc tgccctcggt
     4441 gctgacgtag atctgacgct tcttcacgaa catgaggaag ccctcggcga tcgcaacgcc
     4501 aggcacgcgc cgcagcgctt cgtcgatgcg aaagagcagg tcgagcttcc gctcgagcgg
     4561 cacgtcgaag ggatcgatca cgcagggcga ggtccagacc gcgcgatgag gcggttccgc
     4621 cgcgaggc
//
LOCUS       DASTKC010000268         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_233957, whole genome shotgun sequence.
ACCESSION   DASTKC010000268 DASTKC010000000
VERSION     DASTKC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233957"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFR25_11165"
     CDS             complement(<1..683)
                     /locus_tag="VFR25_11165"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941648.1"
                     /translation="MVTRGPSTLAPADAERLLAGPAASIPSLVLAAGPEEFLRDRLVH
                     AFRAGAVAESSEFQRLEGDDLDAATLLEALTSVSLFADTRRVWIREAAKLEKGAEDAL
                     LSWAAGRGEGVRVLVTTARDVTDLKVLQSLASRASVVTLAPSPAEVKRWAERLVTEAG
                     LKLPSGALDAIASRSNSLLALNQELLKLRLHAGDDGRLPATALDSLAGSRGGASVERW
                     AAAVIAGDVA"
     gene            complement(677..1198)
                     /locus_tag="VFR25_11170"
     CDS             complement(677..1198)
                     /locus_tag="VFR25_11170"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HET9941649.1"
                     /translation="MPRATSPRRLALALFAGAILHAGCGYTVSSVLPAHIKTIAIPTF
                     ANNTVENGLAGDVTESLINGFLADKRLRLERERDADSILRGTILAYRNRVYAYTSQEV
                     ATQYEILLVAQVAFRDVQKNKDIWKEDELTVRTTYNVVAVGSEPAKTEVDGRKDVIQK
                     MTDLIVSRTVQGW"
     gene            complement(1176..2603)
                     /locus_tag="VFR25_11175"
     CDS             complement(1176..2603)
                     /locus_tag="VFR25_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9941650.1"
                     /translation="MAKASILIIDDDETIRYFLPRDLEAEGFQVQTAETGQAGLRALE
                     REPVDLVLLDIRMPDLTGIEVLQKIRDQWPDQIVVMLTGEPDHETAVQAMRLGARDYL
                     TKGKPIREELLLVLDRELSAQRLGREVQHHRRERSQKFSRNFVRGESAAMQRVYSVVE
                     QVATSESTSVLIQGESGTGKELIAHWVHDLSVRRDKPMLEVNCAAIPRELLESELFGH
                     EKGAFTDARQQKQGLLELANGGTLFLDEVGEMSLTIQVKLLRVLERMTFKRVGGTKDI
                     TVSVRVISATNQDLETMVREQRFREDLYYRLKVVPIRVPSLRERREDILPLARHFLEQ
                     FNRAFSKSFKGIDPEAERILLSYPWPGNIRELRNLFERIVLLNHGDRIQAAHLNAAIG
                     PAPIKTDSELSALRQVVETGHFPDNGIDLEGALGAVEREIIQRAFDWTRGNQSRTATL
                     LQMKRDKLRYRMKLYGFHDATSDLS"
     gene            complement(2619..>3690)
                     /locus_tag="VFR25_11180"
     CDS             complement(2619..>3690)
                     /locus_tag="VFR25_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941651.1"
                     /translation="VNLGLKEALDFEARFREEVVENLPLGVVALDVRGHVLTWNRAAE
                     EIIGLTREEALEQPISRLLPERPWYDALVRSLEEGTEVIRVEHDVVRADGTSIPAEIS
                     TAPLRDAEGRIRGAVATLVDVSSLRSMEERIRQLDRLAALGRFASSVAHELRNPLTGI
                     ATGVQYLSRGFPEGDERNESVTFILREVVRLNTIIQDIFTATRPRELRLARVAVRDIV
                     ARTLHGLKPTPEEAGVTIETEGADAWPTVTVDADQLQQVLLNLIQNAIQATPRGGTVS
                     LRADEDRKGWWIEVEDTGAGIEPEHLPRIFDPFYTTRPKGTGLGLFVAHGIVERHRGT
                     IDIHSEPGEGTRFRIRLPHHPA"
BASE COUNT          542 a         1239 c         1274 g          635 t
ORIGIN      
        1 gcgacgtccc ccgcgatcac ggccgctgcc catcgctcga ccgacgcgcc gccgcggctt
       61 cccgcgagcg aatcgagcgc ggtcgccggc aggcggccgt cgtcgccggc gtggagccgg
      121 agcttcagga gctcctggtt cagtgcgaga agactgttcg atcgcgaggc gatcgcgtcg
      181 agcgcgccgg aagggagctt gagacccgcc tccgtcacga ggcgttccgc ccagcgcttc
      241 acctcggccg gactcggcgc gagcgtcacg accgacgcgc gcgacgcgag cgactggagc
      301 accttgaggt cggtcacgtc ccgcgcggtg gtgacgagaa cccgcacccc ttccccgcgg
      361 cccgcggccc acgaaaggag cgcatcctcg gcgcccttct ccagcttggc ggcttcgcga
      421 atccagacgc ggcgggtgtc ggcgaagagc gacacggacg tgagcgcttc gagcagcgtc
      481 gcggcatcca gatcgtcccc ctcgaggcgc tggaactccg aggactcggc gaccgcgccg
      541 gcgcgaaagg cgtggacgag acggtctcga aggaattcct cggggcccgc cgcgaggacg
      601 agcgagggga tcgacgccgc cggtcccgcg agcagacgtt ccgcgtccgc gggcgcgagc
      661 gtggaagggc cgcgcgtcac catccctgga cggtacggct cacgatgagg tcggtcatct
      721 tctggatcac gtccttcctc ccatcgactt cggtcttcgc gggttcggag cccacggcga
      781 cgacattgta cgtggtccgg accgtgagct cgtcctcttt ccagatgtcc ttgttcttct
      841 ggacgtcgcg gaacgcgacc tgggcgacga ggaggatctc gtactgggtg gcgacttctt
      901 gggacgtgta ggcgtagacg cgattccggt acgcgaggat ggtcccgcgg aggatggaat
      961 cggcatcccg ctcccgctcg agcctgagcc tcttgtcggc gaggaagccg ttgatcaggg
     1021 actcggtgac gtcccccgcg agaccgttct cgaccgtgtt gttggcgaac gtggggatcg
     1081 caatcgtctt gatgtgggcg ggcagcacgc tcgagaccgt gtagccgcac cctgcgtgga
     1141 ggatcgcccc cgcgaagagg gcgagcgcga gtcgtctagg agaggtcgct cgtggcatcg
     1201 tggaacccgt agagcttcat ccggtaccgg agcttatcac gcttcatctg cagaagcgtg
     1261 gccgttcggc tctggttccc acgggtccaa tcgaaggcgc gctggatgat ctcgcgctcc
     1321 acggcgccca gcgccccttc caggtcgatg ccgttatccg ggaagtgtcc cgtctccacc
     1381 acctggcgca gcgcgctcag ctcggaatcg gtcttgatgg gagccggtcc gatggcggcg
     1441 ttcaggtggg cggcctggat ccggtctccg tgattgagca ggacgatccg ctcgaacagg
     1501 ttccggagct cgcggatgtt ccccggccaa ggatacgaaa gaaggatccg ctccgcctcg
     1561 gggtcgatcc ccttgaagct cttcgagaag gcgcggttga actgctcgag gaaatggcgg
     1621 gccagcggaa gaatgtcctc gcggcgctcg cgcagcgagg gcacgcggat cggcaccacc
     1681 ttgagccggt agtagagatc ttcccggaat cgctgctcgc ggaccatggt ctcgaggtcc
     1741 tggttggtcg ccgagatcac gcgcacgctg acggtgatgt ccttcgtccc gccgacgcgc
     1801 ttgaaggtca tgcgctcgag cacgcggagg agcttcacct ggatcgtgag gctcatctcg
     1861 ccgacttcgt ccaggaagag cgtgccgccg ttcgcgagct cgaggagccc ctgcttctgc
     1921 tgccgcgcgt ccgtgaacgc gcccttctcg tgtccgaaga gctccgactc gaggagctcg
     1981 cgcgggatcg cggcgcaatt gacctcgagc atgggcttgt cccggcgcac ggagaggtcg
     2041 tggacccagt gggcgatgag ctccttcccg gttccgctct ctccctggat caggacgctc
     2101 gtcgactcgc tcgtcgcgac ctgctccacg acggaataga cgcgctgcat ggccgcgctc
     2161 tcgccgcgca cgaaattgcg cgagaacttc tggctgcgct cccgccggtg gtgctgcacc
     2221 tcgcgcccca ggcgctgggc ggagagctcg cggtcgagga cgaggaggag ctcctcgcgg
     2281 atcggctttc ccttcgtgag gtagtcgcgc gcgcccagcc gcatggcctg gacggcggtc
     2341 tcgtggtccg gctcgccggt cagcatgacg acgatctggt cgggccactg gtcgcggatc
     2401 ttctggagca cctcgatccc ggtgagatcc ggcatgcgga tgtcgaggag gacgagatcc
     2461 acgggctcgc gctcgagcgc gcgaaggccg gcctggccgg tctcggccgt ctgcacctgg
     2521 aacccctcgg cttcgaggtc gcgcggcagg aagtagcgga tcgtctcgtc gtcgtcgatg
     2581 atcaggatcg aagccttcgc catgcggtcc ttcctacctc aggcagggtg gtgcgggagg
     2641 cggatccgga accgggttcc ctctcccggc tcgctgtgga tgtcgatcgt cccccggtgc
     2701 cgctcgacga ttccgtgcgc cacgaacaac ccgagccccg tgcccttcgg gcgggtcgtg
     2761 tagaacggat cgaagatccg agggaggtgc tccggttcga tgcccgcccc ggtgtcctcg
     2821 acttcgatcc accagccctt ccgatcctcg tccgcgcgaa gcgagaccgt ccccccgcgc
     2881 ggggtggcct ggatcgcgtt ctggatgagg ttcaagagca cctgctgaag ctggtcggcg
     2941 tcgactgtga ccgtgggcca tgcatcggca ccttcggtct cgatcgttac tcctgcctcc
     3001 tcgggggtcg gcttgagccc gtggagggtc cgggccacga tgtcccgcac ggccacccta
     3061 gccagccgca gctcccgggg acgggtcgcc gtgaagatgt cctggatgat ggtgttgagc
     3121 cgcaccacct cccggaggat gaaggtgacg ctctcgttcc gctcgtcccc ctccgggaag
     3181 cctcggctca ggtactggac cccggtcgcg atcccggtga gcgggttccg gagctcgtga
     3241 gccacgctcg aggcgaagcg tccgagggcg gccaggcggt cgagctggcg gatccgctcc
     3301 tccatgctcc ggaggctcga cacgtcgacg agggtcgcga ccgcgcctcg gatccgcccc
     3361 tcggcgtcgc gaagcggggc cgtcgagatc tcggccggaa tcgaggtgcc gtcggcccgg
     3421 accacatcgt gctcgacgcg gatcacctcg gtcccctcct ccagacttcg cacgagcgcg
     3481 tcataccagg ggcgctcggg aaggaggcgg gagatgggct gttcgagcgc ctcctcccgc
     3541 gtgagcccga tgatctcctc ggcggcgcga ttccaggtga ggacgtgccc gcgtacatcg
     3601 agggccacga cgccgagcgg caggttctcg acgacctcct cgcggaacct ggcttcgaag
     3661 tcgagcgcct ccttcagtcc caggttgacg
//
LOCUS       DASTKC010000269         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_705370, whole genome shotgun sequence.
ACCESSION   DASTKC010000269 DASTKC010000000
VERSION     DASTKC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705370"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VFR25_11185"
     CDS             complement(<1..1467)
                     /locus_tag="VFR25_11185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9941652.1"
                     /translation="MATSRGRSALPAGTHLGPYEILAPLGAGGMGDVYRARDTRLGRD
                     VALKTLPPEFAADPERLSRFDLEARAVSALSDPHVVTIYDVGVHEGTPFVVFELVEGD
                     TLRKRLEQGALPVASALDLATQVARGLAAAHDRGIVHRDLKPENIVVARGGRVRILDF
                     GLAKRMGGDGAAPSGDASTIGAALTSPGSTYGTVGYMAPEQIREQTVDGRTDLFALGA
                     ILHEMLSGHRAFQGATAADVQTAILSTEPSPLPETARRVPGLERVVRRALEKDPSARP
                     QHARDLAFDLELLASVGPGAADAPAKHRTSRGALLASVCLGAVAAAALAWALTRGPGA
                     SHVQYERITFRNGVVHDARFTPDGHTIVYSAEWDGDSAAVYTAQAGSPETHSLGYPNA
                     VLLAVSSGGDLGIGLRTFSRPHFTRQGTLSRVPLAGGAPRAIVDSVVAADWAHDGSLA
                     VLIVSGGRYRLEWPPGRVLRETPGWMSHPRLAPDGTQIA"
     gene            complement(1498..2178)
                     /locus_tag="VFR25_11190"
     CDS             complement(1498..2178)
                     /locus_tag="VFR25_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9941653.1"
                     /translation="MDTILCHTVRSTRAAPSKLWISDLTSALVRSTVRSSERPFRADV
                     GVRLLEGDIVTDLLISVEERRILLATMEPPIVEIVPDEPFSSILRILRRALPTNQVIG
                     DAIEEEEAAVLRSQSKSENGSPLWPDCVPGAAAGEFVFYEDEPVPIATPEPVLPPGTK
                     QREVTRVTLHVFVDRSGRVCFAKVIKGMEPFASSAIEAARHWTFKPAMSHGKPVGVWV
                     ELPFTFKP"
     gene            complement(2617..>2916)
                     /locus_tag="VFR25_11195"
     CDS             complement(2617..>2916)
                     /locus_tag="VFR25_11195"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017972288.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9941654.1"
                     /translation="EIVKLLQTGSAFYAPASSAVQMAESILRDKKRLLPAAAYLQGEY
                     GMRDLYLGVPVILGSKGVEKIVELKLDAADQAALAKSADDVKKGIADLALEPVAR"
BASE COUNT          502 a          988 c          972 g          454 t
ORIGIN      
        1 ggcgatctgg gtgccgtcgg gcgccaggcg cgggtggctc atccatccgg gcgtttctcg
       61 aagcacgcgc cctgggggcc actccagccg atatcggcct ccggacacga tcaagaccgc
      121 aaggctccca tcgtgcgccc agtcggcggc cacgacgctg tccacgatcg cccgcggcgc
      181 gccacctgcc aggggaacgc gcgaaagggt tccctgtcga gtgaagtgcg gccggctgaa
      241 cgtgcggaga ccgattccga gatcgcctcc actcgagacc gcgaggagga cggcgttggg
      301 atatccgagc gagtgcgttt cgggagagcc cgcctgggcg gtgtacacag ctgccgagtc
      361 gccatcccac tccgcgctgt agacgatcgt gtgcccgtca ggggtgaacc gggcatcgtg
      421 gaccactccg tttcggaacg tgatgcgctc gtactggacg tgggacgcgc ccggtccgcg
      481 ggtgagcgcc catgccagag cggccgcagc caccgctccg agacacacag acgcgaggag
      541 ggcgccccgg ctcgtgcgat gcttggcggg cgcgtcggcc gcccctggac cgaccgaggc
      601 caggagctcg aggtcgaagg cgagatcgcg cgcgtgctgc ggacgtgcgg acgggtcctt
      661 ctcgagcgcg cggcggacca cgcgctcgag tcccggcacc cggcgcgccg tttccggcag
      721 cgggctgggc tcggtggaga ggatcgcggt ctgaacgtcc gcggcagtcg cgccctggaa
      781 cgcgcgatgc cccgacagca tctcgtggag gatcgcgccc agcgcgaaga gatcggttcg
      841 gccgtcgacg gtctgctcgc gaatctgctc cggcgccatg tagccgaccg tgccgtacgt
      901 ggagccggga ctcgtgagag ctgcaccgat cgtggacgcg tcaccggaag gagcggcacc
      961 atcgcccccc attcgcttcg ccagcccgaa gtcgaggatc cgcacccggc cgccgcgggc
     1021 cacgacgatg ttctccggct tgagatcgcg atgcacgatc ccgcgatcat gtgcggcagc
     1081 gaggccgcga gcgacctgag tcgcaaggtc gagcgcggac gcaaccggaa gcgcgccctg
     1141 ctcgagccgc ttccggagcg tgtccccttc caccagctcg aacacgacga acggcgtccc
     1201 ttcgtgcaca ccgacgtcgt agatcgtgac cacgtgagga tcgctgagtg cgctgacggc
     1261 gcgcgcttcg agatcgaacc ggctcagacg ctcgggatcg gccgcaaatt cgggcgggag
     1321 ggtcttcagc gcgacatcgc gcccgagccg tgtgtcgcgg gcgcgataca cgtcccccat
     1381 cccgcccgcg ccgagcggcg ccaggatctc gtagggaccg aggtgggtcc cggccggtag
     1441 ggcgctgcgt cctctagacg tagccatatc gataccccag ctgttgggcg ctccccctca
     1501 cggcttgaac gtaaacggca attcgaccca cacgccaacg ggctttccat gggacatcgc
     1561 aggcttgaac gtccaatgtc gtgcagcctc gatcgctgac gaagcgaaag gctccatacc
     1621 cttgatgacc ttggcaaagc aaacgcggcc cgaacgatcg acgaagacgt gcaaagtaac
     1681 ccgagtgact tcccgctgct tcgttcctgg aggcagaacg ggctccggcg ttgcgattgg
     1741 aacgggctca tcctcgtaga agacgaactc gcccgccgcg gcccctggaa cgcagtcggg
     1801 ccagaggggt gaaccgttct cggacttgga ttggctacgt agtaccgctg cctcttcctc
     1861 ctcaatggca tcaccgatga cctggttcgt cggcagcgcc cgtcggagga tacgaaggat
     1921 cgaggagaac ggctcgtcgg gcacgatctc gacgatcggg ggttccatgg tcgccagaag
     1981 gattcgccgt tcctcgactg agatgagcag gtcggtgacg atgtcgcctt cgagcaagcg
     2041 cacgccgacg tcggcgcgaa acggacgctc gctcgagcgc acggtcgaac gcaccagtgc
     2101 ggacgtcaga tccgaaatcc agagcttgct cggcgcggcc ctggtcgatc gcacggtatg
     2161 gcacaggatc gtgtccacga ataccgatgc tcggagggtg cacccaggcg atggcaggct
     2221 gtccacctcg tagacccacc ccgtgctcgc ctgcctcaat cgctcggcac tggccggggc
     2281 caggcgcggg agaggaatcg gggtcatggt ctgagtcggc ggtggggact ccggtgacgg
     2341 aatcgtggca ggactctctg ctcgcgtcgg cgcggcgact gcgagcaccc ctaccaagcc
     2401 gaatgtactt gctacgcgca ctagactcat ccagccgtcg atcgaatgac tcgcgaatgg
     2461 cgctcgagcc ctatggccca aaaaaagcgg ggccaagagg agcgttccga gcgatgtctt
     2521 cggaacgacc cgcttccccg caaagcccac ggcgatgtgc ccccggaaca cgctcgaagc
     2581 acctcgctct cgcgcgcttc cgcgcgcgcc caacggttag cgcgcgaccg gctccagcgc
     2641 caggtccgcg atgcctttct tcacgtcgtc cgccgacttc gcgagcgccg cttgatccgc
     2701 cgcatccagc ttcagctcca cgatcttctc cacgcccttg gatcccagga tcacgggaac
     2761 gccgaggtag aggtcgcgca tgccgtactc gccttgcaga tacgcggcgg ccggaaggag
     2821 gcgcttcttg tcgcgcagga tcgactccgc catctggacc gcggaggaag ccggcgcgta
     2881 gaaggcgctt cccgtctgga gcagcttcac gatctc
//
LOCUS       DASTKC010000270         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_235803, whole genome shotgun sequence.
ACCESSION   DASTKC010000270 DASTKC010000000
VERSION     DASTKC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235803"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..806
                     /locus_tag="VFR25_11200"
     CDS             156..806
                     /locus_tag="VFR25_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941655.1"
                     /translation="MSLHPSHDIGVWIAALLTLGIFSFLYRDNPVYKFCEHLFVGVSA
                     GYYVVLTMISSVYPNLFLPLFSSFAKDYHWLLIVPLILGIMLFSRFFPRGDWLSRWPI
                     AFVLGVYPALRITGFGQGDFVEQIHGTILPLWVPGSIGASIGNILLVVGLVATLIFFF
                     FSKEHKGALGGTARLGIYFLMVSFGASYGYTVMARVSLLIGRVMFLLDDWLGALRF"
     gene            814..1602
                     /locus_tag="VFR25_11205"
     CDS             814..1602
                     /locus_tag="VFR25_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941656.1"
                     /translation="MSAPLRLLRLPHLEEDGSLQMAIDAALLDWASRGLDRAVFRTYD
                     WNPPAISLGRAEPFPDGWDLAAIRAAGLDVVRRPTGGDAVLHDGELTFALAASLPGPW
                     NLGPRAYANLVAEALADAVAAFGLKAERVLERGIAARPGADPCFARASSGELRSGAFK
                     VAGIASRFTRGAALCHASVPLTPRHRDIARFRGDRPGEREALAEHARSIAEILGRSVP
                     AVALGDVLATKVAGRLGASLEVAPLDDCVPGGVERARVRAVETA"
     gene            1599..2087
                     /locus_tag="VFR25_11210"
     CDS             1599..2087
                     /locus_tag="VFR25_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941657.1"
                     /translation="MIARRSVWTWISLYATAMALVEAACVVTLKQLFYPGGWAPPFAP
                     MPAGPLLLEQVREIATLVMIAAVACLAGRTPRARLAAGLWIFGIWDILYYAFLRWLTG
                     FPRSLADVDVVFLVPIAWIVPVWVPFVTSAAALALALWLRASVPHASLAQPPHPKAEI
                     RR"
     gene            2116..>2864
                     /locus_tag="VFR25_11215"
     CDS             2116..>2864
                     /locus_tag="VFR25_11215"
                     /inference="COORDINATES: protein motif:HMM:NF018155.4"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9941658.1"
                     /translation="MSRSQGRLVGLVVLDGWGINPKKDGNAVALARTPVMDALLASRP
                     SATLTTCGEAVGLPVGQMGNSEVGHLNLGAGRIVYQDLTRIDQAVKDGSLEKNPVWGA
                     ALDRARVEGTLHLIGLLSPGGVHSHERHLAAMIRHAIARGVARIRVQAFLDGRDTAPR
                     SAGASIRGLEAVLRDVKDGKIATISGRYYAMDRDKRWDRQRRAWDAIVDASAEHVAGD
                     ALTALRDAYARGENDEFVAPTVIGTGASVRDG"
BASE COUNT          358 a         1031 c          980 g          495 t
ORIGIN      
        1 atggcgggcg cggcggaata cgaggtcctc gtgggcgccc ccggactcgc gacccggggc
       61 atggacgccc agtcgctcgc gcatgtgttc atcgcgttca tgatcattct cggcaacctc
      121 gccttcgtca ccggaacgaa gaaggggaac gggcggtgag cctccacccg agccacgaca
      181 tcggcgtctg gatcgcggcg ctcctcacgc tcggcatctt cagcttcctg taccgggaca
      241 atccggtcta caagttctgc gagcacctgt tcgtcggcgt ctcggcaggc tactacgtgg
      301 tgctcacgat gatctcgtcg gtgtacccga acctgttcct gccgctcttc tcctcgttcg
      361 cgaaggacta tcactggctc ctgatcgttc cgctcatcct cgggatcatg ctcttctcgc
      421 gcttcttccc gcgcggggac tggctctcgc ggtggccgat cgccttcgtg ctcggcgtct
      481 atcccgcgct ccgcatcacg ggattcggcc agggagactt cgtggagcag atccacggca
      541 ccatcctgcc gctctgggtg ccggggagca tcggtgcgag catcggcaac atcctcctcg
      601 tcgtcggtct cgtggccacg ctgatcttct tcttcttctc gaaggagcac aaaggcgctc
      661 tcggggggac ggcgcggctc gggatctatt tcctcatggt gagcttcggc gcgtcgtacg
      721 gatacacggt gatggcgcgc gtctcgctcc tgatcggccg cgtgatgttc ctgctcgacg
      781 actggttggg cgccctccgg ttctgatcgc gccgtgagcg ccccgctccg gcttctccgc
      841 cttccgcacc tcgaagagga cggctcgctc cagatggcga tcgacgccgc gctcctcgac
      901 tgggcgtcgc gcggcctgga tcgcgccgtc ttccgcacct acgactggaa tcctcccgcg
      961 atcagcctcg gccgcgccga gccctttccc gacggctggg acttggccgc gatccgcgcg
     1021 gccgggctcg acgtcgtgcg gcgcccgacg gggggcgacg ccgtgctcca cgacggcgag
     1081 ctcacgttcg cgctcgccgc gagcctcccg gggccgtgga acctcggtcc ccgcgcgtac
     1141 gcgaatctcg tcgccgaagc gctcgcggat gccgtggccg cgttcggcct gaaggcggag
     1201 cgcgtcctcg agcggggcat cgccgcgagg cccggcgccg acccgtgctt cgcccgcgcc
     1261 tcgagcgggg agctccggtc gggcgcgttc aaggtcgccg ggatcgcgtc gcgcttcacg
     1321 cgcggggcgg ctctctgtca cgcatccgtg cctctcacgc cacgtcaccg ggacattgcg
     1381 cgcttccgcg gcgaccgtcc aggagagcgg gaggccctgg ccgagcacgc tcgttcgatc
     1441 gccgagatcc tgggacgctc cgtgcctgcc gtggcgctcg gagacgtgct cgccacgaag
     1501 gtcgcggggc gactcggagc gtcgctcgag gtggcgccgc tcgacgactg cgttcccggg
     1561 ggcgtggagc gtgcgcgggt ccgggccgtg gagaccgcat gatcgcgcgc cgctcggtct
     1621 ggacctggat cagcctctac gccaccgcca tggcgctggt cgaggccgcc tgcgtggtca
     1681 ccttgaagca gctcttctat ccgggaggtt gggcgcctcc gttcgctccg atgcccgcgg
     1741 ggccgctcct cctcgagcag gtgcgggaga tcgcgaccct cgtgatgatc gcggccgtcg
     1801 catgcctggc gggccgtacc ccccgcgctc gccttgcggc cgggctctgg atcttcggca
     1861 tctgggacat cctctactac gcgttcctcc gctggctcac cggctttccc cgatcgctcg
     1921 cggacgtgga cgtcgtcttc ctcgtgccca tcgcatggat cgtcccggtg tgggttccgt
     1981 tcgtcacgag cgccgcggcc ctggcgctgg cgctctggct ccgcgcctcc gtgccgcacg
     2041 cgtccctcgc ccaaccgccg catcccaagg ccgagatccg ccgctgaccg acgagcctgt
     2101 ggtaccgttt cgcccatgag ccgaagccag gggcggctcg tcgggctcgt cgtgctcgac
     2161 ggctggggaa tcaatccgaa gaaggacggc aacgcggtgg cgctggcgcg cacgcccgtc
     2221 atggacgcgc tcctcgcgtc ccggccgagc gccacgctca cgacgtgcgg cgaggccgtc
     2281 ggactcccgg tggggcagat ggggaactcc gaggtgggcc atctgaacct cggcgcgggc
     2341 cgcatcgtgt atcaggacct cacgcgcatc gatcaggcgg tgaaggacgg gtcgctcgag
     2401 aagaacccgg tctggggcgc cgcgctcgat cgcgcgcgcg tcgagggcac gctccacctg
     2461 atcgggctcc tctcgcccgg cggcgtccac agccacgagc ggcacctcgc cgcgatgatc
     2521 cgtcatgcga tcgcgcgcgg cgtggcgcgg atccgcgtgc aggcgttcct ggacggacgc
     2581 gacacggcgc cgcgctcggc cggcgcgtcg atccgcgggc tcgaggccgt gctccgcgac
     2641 gtcaaagacg ggaagatcgc gacgatctcc ggacgctact acgcgatgga tcgcgacaag
     2701 cgctgggacc ggcaacggcg ggcatgggat gcgatagtcg acgcctcggc ggagcacgtc
     2761 gccggcgatg cgctcactgc gctgcgcgac gcctatgcgc gcggcgagaa cgatgaattc
     2821 gtcgctccga ccgtgatcgg caccggtgcc tccgtgcgcg acgg
//
LOCUS       DASTKC010000271        12738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_944660, whole genome shotgun sequence.
ACCESSION   DASTKC010000271 DASTKC010000000
VERSION     DASTKC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944660"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="phoU"
                     /locus_tag="VFR25_11220"
     CDS             complement(<1..506)
                     /gene="phoU"
                     /locus_tag="VFR25_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9941659.1"
                     /translation="MERHFHHDLHTLQDRLGEMAGRVETALSKALEAIHRRDTRLARE
                     VVDEDEAIDRIELEVERRCLDFLGLQQPVARDLRFLVASIRVSNYLERMGDHAVNLGQ
                     SAQKLAALPPGKGMAEVMSMGERTMRMLRDAVSAWLGHDAALAKRVCESDAEIDTFKA
                     QIFAKFSS"
     gene            complement(551..1339)
                     /gene="pstB"
                     /locus_tag="VFR25_11225"
     CDS             complement(551..1339)
                     /gene="pstB"
                     /locus_tag="VFR25_11225"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET9941660.1"
                     /translation="MSAQAASPSTDVAPILQTQGLSLWYGAHHALKSVDLGIGRNRIT
                     AIIGPSGCGKSTLLRCFNRMNDLFPPIRYDGSILFHGQDVLGSSTDLVALRRRVGMVF
                     QRANPFPMSIFQNVAYGPRLQGVGNRRKLEEIVESALRRSALWEEVKDRLNRSALGLS
                     GGQQQRLCIARALAVEPEVLLLDEPASALDPTATAKIEELVLEIKSSVTVAIVTHNMQ
                     QAARVSDETAFMLNGKLIEEGPTRTLFTSPRERLTEDYITGRFG"
     gene            complement(1471..2880)
                     /locus_tag="VFR25_11230"
     CDS             complement(1471..2880)
                     /locus_tag="VFR25_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9941661.1"
                     /translation="MRSLTRKPFTRLALAFVAAAILTAWLMPGALAAGLAAAVALPLV
                     LFGLALLVGFVSFRSLDRRFATVAESAEAQAGGDLATLAPVDEHDEVGQLARALNDLA
                     VTHNTRVLELRAERDAGESILGHLPLGLALLEPDLTIRHANALFWDMMGLTPPPGEVR
                     LSAARQPALEQITLDALRRGRGLVRDVTLYRGGRREYEARVTPIAVEGSRPESLLLSV
                     EDLGPEKAMAALRREFVANASHELRTPLTSIRGYAETLLGGALDDASHRVRFVETIRD
                     QASRMEALVVDLLELADLERPDAPLDLKDWDLSLVARDLGSTFAELAARRGLELAVEA
                     RPGVHARVDRKRLELALRNLLDNAVKYTEAGSITLRVEPVGRDVVRVMVTDTGRGVSA
                     EHLPRLFERFYRVDRGRSRDQGGTGLGLSIVKHVVQLHGGKLGVESKPGEGSTFWFEI
                     PTAGPGGQTPNPAGGPGAA"
     gene            complement(2919..3617)
                     /locus_tag="VFR25_11235"
     CDS             complement(2919..3617)
                     /locus_tag="VFR25_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9941662.1"
                     /translation="MPTTARSTILVIDDEPEICELVSYNLSREGYRVLSERDGESGLE
                     RVFQSPPDLLVLDLLLPHKSGLEVLRAIRAESRTRTLPVLILTARGTEMDKLVGFEHG
                     ADDYLTKPFSPKELVARVSAILRRTRGEERIAPTEIGPLRFDRERHQVFMNERELRLT
                     PTEYRLLEYLLERPGRVFTREQLLDKVWGLGYFGETRTVDVHVRRLRAKLGKEASLIR
                     TVTGVGYAAELPKG"
     gene            3782..5257
                     /locus_tag="VFR25_11240"
     CDS             3782..5257
                     /locus_tag="VFR25_11240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9941663.1"
                     /translation="MRPSRIALVLVLAAALRLVHVSAPILGVHSWRQADTAAMARNFH
                     ENGYRFLYPQVDWGGSGPGYVECELPAFPYAVSLGYGLFGPWEAWGRLFAALLSTLTV
                     YLLYRLARDAYGDERAALAGAFVFAVLPLSVYYGRAFMPEAAMLCASVAALHAFDRWT
                     ATGSRRWYLLAAAATAMACLLKLPCLYLGLPLLYLAWRSWGTALVRRLDLWLFAALVL
                     APVALWYAHAHALYRGGGVTFGIWEYGSDKWGNWRLVASWDFWNGVLLRSLAERHLTW
                     AGVPLALAGLLLPRRGRRAGLVDWWLLALGVYLIVVARGNYVHEYYQLPLIPPLSIAM
                     GRAIAAAFAPGARRLVRAVAATALLAAMVALGAGRLASYWRREDPARSPLYRLAHEVE
                     RRTPSGARIVTLDQGNPTLLYLAHRKGWHASPEDLASDTLRVRAAGGALYAAGLRQDL
                     HGSTALENGLEAGLGRDLLASDPDAFLLELGPPRAGGTSGR"
     gene            5344..6966
                     /gene="secD"
                     /locus_tag="VFR25_11245"
     CDS             5344..6966
                     /gene="secD"
                     /locus_tag="VFR25_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET9941664.1"
                     /translation="MTPSDRRKLFGTLIGLAICIWALWPTFTFYSLSPAERREVLQAR
                     PAAATNDVDRARLEKMAKLRDGAIKLGLDLQGGMYLLLEVDKSKLGPAEAKNAVDQAM
                     QIIRNRIDQFGVAEPTIQKQGDNRILVQLPGLLDQGRAKELIGQTALLEFKIVKTDDE
                     AKALFNRIDAYFTRKMGGAAADSTGPDSLRHPFTGRFLTVAQGSESFALSSEVAAIDS
                     MLTVLRSDSTFTTDAAIAWDAHETELQGRSGRNLYVVDKEPQMRGTEVASAQMRLDLD
                     QERPGSPGVSFNLTSKGGAVFADVTSRNVQRRLAIVLDNRVQSAPNIRERIPGGHGSI
                     TGSFTEEEAQNLSIVLRSGALPAPVNIVEERSVGASLGQDSIEKGVRAGAVGALLVIV
                     FMIVYYRLSGLVAVAALLLNIIGLLAAMAGFHATLTLPGIAGIVLTIGMAVDANVLIF
                     ERIREELRNKRTVLNAIDMGYSRAWRTILDAHVTTLLSAVALMSFGTGPIRGFAITLS
                     IGLIINMITAVFVSKMIFDAWSLRRKVSTISI"
     gene            6980..7933
                     /gene="secF"
                     /locus_tag="VFR25_11250"
     CDS             6980..7933
                     /gene="secF"
                     /locus_tag="VFR25_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET9941665.1"
                     /translation="MLEILHGVNVDWMGKRHIAYWISGICVAISIVSLIMHGGPRYGV
                     DFTGGTLVELQVTPPLPVDNVRRAVDQAGFTGSEIQALNVPGQYLVRIGLTELHKVDP
                     APRVREAVAITNPGATVEIRKVETVGPRVGSELRGSALKAIFLALGLILVYVAFRYDW
                     RYSLGAVVALFHDVFIALGAVSLTNKEVTLTIVAALLTIAGFSINDTIVVFDRIRERK
                     QTLRREHPAKVMNIAVNETLSRTIITSFTVFLTVLALYLLGGEVLHDFSFTLLVGIVF
                     GTYSSVYVASGLALDTQLYLEGQGLRRKQAPAPTKTAKTRG"
     gene            7980..8495
                     /locus_tag="VFR25_11255"
     CDS             7980..8495
                     /locus_tag="VFR25_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941666.1"
                     /translation="MARPRTGLGFGAVTLAAAALLASPDAARFASAEGTPPPAETAPP
                     VPRVQDSPLKLFAAVEDAWLASDAERLADMVDTTVVRITLEPGTPPTSAITRNAALFL
                     FRDQLRLVKTKAFRVTRLEIGQKSKPTATATAIWSADWGGRRGMRDLKVAFVAVPVGS
                     RWVLTEVRAND"
     gene            8526..9404
                     /locus_tag="VFR25_11260"
     CDS             8526..9404
                     /locus_tag="VFR25_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9941667.1"
                     /translation="MSDSRSEHTTADVLYASDLHGHTGLYRELLETAWRLRVRAVILG
                     GDLAPHADVATQRRFYEEFLVPLLRDYRGKPGAAEIFHIPGNDDWKASLTPLLEASLS
                     GTHAIHGRVVPFLDHGFIAGLASVPITPFPMKDWDRWEEGLAAAAKMEGFRSEPDGGL
                     RAYTFRGRERRESLAADLDALEREMPVDGTEPLICVLHGPPYGTALDQIARGVHVGSR
                     ESRRFLERRGPLLGLHGHIHESPAISGRFAERLGRTICINAGQRMGSALHAVWFRLDD
                     IPGTLTHSLLGPAQLG"
     gene            9691..11142
                     /locus_tag="VFR25_11265"
     CDS             9691..11142
                     /locus_tag="VFR25_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET9941668.1"
                     /translation="MEAAYRELGVDSMGPAGYGQDVPFLVRTVDEPSSSVTLWPEEGS
                     PIALRFGDDATLIRSAPLVRDVEAPLVFVGYAVVAPEAKYDDLEWQDLKGKIAVFVSS
                     GPAAVPDPMRAHVQFAAERWAHLKAAGAIGAISIPNPATMEAGWSRYILNRAQPAMAL
                     DDKADHELDGQRFGMRLNPACADTLFKDSQHTWAEIAAMAKRGDQLPTFDLPMRLKAH
                     VEAKTWRVRSPNVIGVIHGSDPTLRNEYVLLTAHLDHLGVGAPVDGDSIYNGAMDNAS
                     GVATLLEVARALRQGSAPKRSVILAALTGEEEGLYGSRYFALHPLVPMSRIVAEINVD
                     MVLPIVPLTHIVVFGLNESSLGLRATEAAQRHGVATQKDPEPQRNLFIRSDQYNLIRA
                     GVPSLAFVTGFVPDSPAEKQLKEWRHNRYHAPSDDLDQPIDHQAAALWNEILLDLVRD
                     TADRAEKPRWNSDSFFARFAKEPATNSPRAARP"
     gene            11139..11789
                     /locus_tag="VFR25_11270"
     CDS             11139..11789
                     /locus_tag="VFR25_11270"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9941669.1"
                     /translation="MITIARAPLRREVRRIVLDRILRGEIPPGGGINESELAIDIGVS
                     RTPLREALLGLEREGFLRSETGRGFFAAALTAEDAEELYPILWTLEGLALASSPSPTP
                     ARKAELERINADLARYERIPERALDFDRLWHDTLLAACPKRRLLAMTEVLKDQARRYE
                     RAYMADSGRVFVSTRQHEEILEALEKDERERAVRLLETNWRVSLDFLLPWLRKARP"
     gene            11786..>12738
                     /locus_tag="VFR25_11275"
     CDS             11786..>12738
                     /locus_tag="VFR25_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9941670.1"
                     /translation="MSARAAAAREPLAPEFVLEILQSLVRIPSVNPTLAPEEGGGEAE
                     IAGFVRDWLDARGIEARLEEAAPGRPNVFAAVGADAGPTLVLCAHLDTVGTAGMTIPP
                     FEPRVEGNRVYGRGSFDMKGGVAAILAAAVALRDAGLQGRVLLALVADEEYTSLGADD
                     FVRRHRADGCILTEASEGRLITATKGFAWIEARTEGRAAHGSRWDIGVSAIARMGRIV
                     AALDDLDRRVLRQRTHPLVGPASLHCAKIDGGVGLSTYAPACTLTVERRTLPGETPES
                     ALEEIRSTIAGAGEEANLSIVFARPPLTCDPRAKVARAAREA"
BASE COUNT         1910 a         4468 c         4312 g         2048 t
ORIGIN      
        1 tccgaagaga acttcgcaaa gatctgcgct ttgaacgtgt cgatctcggc gtcgctctcg
       61 cagacccgtt tcgcgagcgc ggcgtcgtgg ccgagccagg cggagaccgc gtcccggagc
      121 atgcgcatcg tccgctcgcc catggacatc acttcggcca tgccctttcc cggaggaagc
      181 gcggcgagct tctgcgcgct ctggccgaga ttcaccgcgt ggtcccccat ccgttcgagg
      241 tagttcgaga cgcggatcga ggccacgagg aaccggaggt cgcgtgcaac cggctgctgg
      301 aggccgagga aatcgaggca tcgccgctcc acctcgagct cgatccggtc gatcgcctca
      361 tcctcgtcca cgacctcgcg cgcgaggcgg gtatcgcggc gatggatcgc ctccagcgcc
      421 ttcgacagcg ccgtctcgac gcgccccgcc atctcgccca ggcgatcctg gagggtatgg
      481 agatcgtggt ggaagtgacg ttccatgaca tgaacctcgg ggtccggagc cgcgtacggg
      541 aggacgcgga tcaaccgaag cggccggtga tgtagtcctc ggtcagccgc tcgcgcggcg
      601 acgtgaagag cgtgcgcgtg ggtccctctt cgatgagctt cccgttgagc atgaaggcgg
      661 tttcatcgga gacccgggcc gcctgctgca tattatgcgt cacgatcgcc accgtgacgc
      721 tggatttgat ttcgagcacc agctcctcga tcttcgcggt cgcggtgggg tcgagggccg
      781 aggcgggctc gtcgaggagc aggacctcgg gctccaccgc gagcgctcgg gcgatgcaga
      841 gccgctgctg ctgcccaccg gagaggccaa gggccgagcg gttcagccgg tccttcacct
      901 cttcccagag cgccgaacgc cggagcgcgg attccacgat ctcctcgagc ttccggcggt
      961 tgccgacccc ctggagccga ggcccgtagg ccacgttctg gaagatcgac atcgggaacg
     1021 gattggcgcg ctggaagacc atgcccacgc ggcgccggag cgccacgagg tcggtcgaag
     1081 atccgagcac gtcctgcccg tggaagagaa tcgatccgtc gtagcgaatg ggcgggaaga
     1141 ggtcgttcat ccggttgaag catcgaagga gcgtcgactt tccgcacccg gacgggccga
     1201 tgatcgccgt gatccgattt cgtccgatcc cgaggtccac actcttgagt gcgtggtggg
     1261 cgccgtacca gagcgatagg ccctgggtct ggaggatcgg tgccacgtcc gtcgaggggg
     1321 aggctgcctg ggcgctcatg gacggctccg gagcttcgga ctggggcggc gcagccgggt
     1381 tgggccggac gttgagtagc gcggttcgtg ccatgtattc atgctagtcg agcccccggg
     1441 ctcggccatg tcggacttgt tacgatccgg ttacgccgca ccgggcccgc ccgccggatt
     1501 cggcgtctgc ccgcccggtc cggcggtcgg gatctcgaac cagaaggtgc tcccctcccc
     1561 gggcttgctc tcgaccccga gctttccccc gtggagctgg acgacgtgct tcacgatgga
     1621 gagcccgagc cccgtcccac cctggtcccg cgagcggccc cggtcgaccc ggtagaaccg
     1681 ctcgaagaga cgaggcaggt gctccgcgga cacgccgcgg ccggtgtcgg tcaccatcac
     1741 ccgcaccacg tcgcggccaa ccggttcgac cctcagcgtg atcgaccccg cttccgtgta
     1801 cttgaccgcg ttgtcgagga ggttacgaag cgccagctcg aggcgcttcc ggtccacgcg
     1861 cgcgtgcacg ccggggcgcg cttccaccgc gagctcgagc ccgcggcgtg ctgcgagctc
     1921 cgcgaaggtg gacccaagat cgcgagcgac caggctcaaa tcccagtcct tgaggtcgag
     1981 cggcgcgtcc ggacgctcca ggtccgccag ctcgagcagg tccaccacga gagcctccat
     2041 ccgcgaggcc tggtcccgga tcgtctcgac gaagcgcacc cggtgggacg cgtcgtcgag
     2101 cgcgccaccc aggagcgtct ccgcgtagcc gcgaatcgag gtgagcggcg tgcgcagctc
     2161 gtgcgaggcg ttcgccacga actcgcgcct caaagccgcc atcgccttct cggggcccag
     2221 gtcctccacg ctcagaagga gcgattcggg gcgggaccct tccaccgcga tcggcgtgac
     2281 tcgcgcctcg tactcgcggc ggccgccgcg atagagggtg acgtcgcgca cgagcccgcg
     2341 cccgcggcgg agcgcgtcga gcgtgatctg ctcgagcgcg ggctgcctcg cggcggagag
     2401 ccgcacctcg cccggcggcg gcgtgagccc catcatgtcc cagaagagcg cgttggcgtg
     2461 gcggatcgtg aggtcgggct cgaggagtgc gagcccgaga ggcaggtgcc cgagaatgga
     2521 ttcgccggcg tcgcgctccg cgcggagctc gaggacgcgc gtgttgtggg tgacggcgag
     2581 atcgttgagc gcgcgcgcga gctggccgac ctcgtcgtgc tcgtcgacgg gcgcgagcgt
     2641 tgcgagatcc cctccggcct gagcctccgc gctctcggcc accgtcgcga agcgccggtc
     2701 cagcgagcgg aacgagacga atccgacgag gagtgccaag ccgaagagaa cgaggggcag
     2761 ggccacggcg gcggcgagac ctgccgcgag cgcgcccggc atgagccacg cggtcaggat
     2821 ggcggccgcg acgaacgcga gtgcgagcct ggtgaacggc ttccgcgtga gcgagcgcac
     2881 ggcctcagcg tacaccgccg gggcgttcgg ggtgacgctt atcccttcgg gagctcggcg
     2941 gcgtagccga cgccggtcac ggtgcggatg agcgaagctt ccttgccgag tttggcccgc
     3001 aggcggcgca cgtggacgtc caccgtccgc gtctcgccga agtagccgag cccccagacc
     3061 ttgtcgagga gctgctcgcg cgtgaagacg cgccccggcc gctcgagcag gtactcgagc
     3121 aggcggtact cggtcggcgt gaggcgaagc tcgcgctcgt tcatgaagac ctggtgccgc
     3181 tcgcggtcga accggagcgg cccgatctcg gtcggcgcga tccgctcctc accgcgagtc
     3241 cggcgcaaga tcgcgctcac gcgagcgacg agctccttcg gcgagaacgg cttggtcagg
     3301 tagtcgtccg cgccatgctc gaagccgacg agcttgtcca tctcggtgcc gcgcgcggtg
     3361 aggatgagca cggggagcgt gcgcgtgcgg gactcggctc ggatggcgcg gaggacttcc
     3421 aggccgctct tgtgcgggag caggagatcg agaacgagca gatcgggagg tgactggaac
     3481 acgcgctcga gcccggattc accgtcgcgc tcggagagaa cgcggtatcc ctcgcgagag
     3541 aggttgtagc tcaccagctc gcagatttca ggctcgtcgt cgatgaccag aatcgtcgag
     3601 cgtgcggtcg tcggcacctt ggccccgtgt cgtgcggcgg gtcgtcgtgg gatcgacgtc
     3661 ggcaagtcta ccatgcgtgc gacgctacgc ccggccggtt accgtcgcgc gtcggcctcg
     3721 tgtccaagct gttacggtcc ggcgggtagg aaagaaccgg ccggcgtggc acactccgcc
     3781 tgtgcgcccg tcccggatcg cgctcgtcct cgtcctcgcg gccgctctca ggctcgtgca
     3841 tgtgagcgcc ccgattctcg gggtccactc ctggcgtcag gccgacaccg cggcgatggc
     3901 gcggaacttc cacgagaacg gctaccggtt cctgtatccg caagtggact ggggcggctc
     3961 cggaccgggc tacgtcgagt gcgagcttcc cgcgtttccc tatgcagtct cgctcgggta
     4021 cggcctcttt ggcccatggg aagcgtgggg gcgcctgttc gcggcgctcc tctcgacgct
     4081 cacggtctac cttctgtacc gcctcgcgcg cgacgcctac ggcgacgagc gggcggcgct
     4141 cgcgggggcg ttcgtgttcg ccgtgctccc gctctcggtc tactacggcc gcgcgttcat
     4201 gcccgaggcc gcgatgctct gcgcgagcgt cgccgcgctc catgcgttcg atcggtggac
     4261 cgccacgggc tcgaggcgct ggtatctcct cgccgcggcc gcaaccgcga tggcctgcct
     4321 cctcaagctc ccctgtctct acctcgggct cccactcctc tacctcgcgt ggcggtcctg
     4381 ggggaccgcg ctcgtgcggc gcctggacct ctggctcttc gcggcgctcg tcctggctcc
     4441 ggtcgcgctt tggtatgcgc acgcgcacgc actctatcga ggcggcgggg tcaccttcgg
     4501 gatctgggag tacggctcgg acaagtgggg caactggcgg ctggtcgcga gctgggattt
     4561 ctggaacggc gtcctgctgc ggagcctcgc ggagcgccat ctcacctggg ccggcgtgcc
     4621 gctcgcgctg gcggggctac tcctccctcg tcgtggacgc cgggcgggtc tggtggactg
     4681 gtggctcctg gcgctcggcg tctacctgat cgtcgtggcg cgtggaaact acgtccacga
     4741 gtactaccag ctcccgttga tcccgcccct ctcgatcgcg atgggccgcg cgatcgcggc
     4801 ggcgtttgct ccgggggccc gccgcctcgt gcgcgcggtc gccgcgacgg cgctcctcgc
     4861 ggcgatggtg gccttgggcg ccggaaggct cgcctcctac tggcgccgcg aggatccagc
     4921 gcggtcgcca ctctaccggc tggctcacga ggtcgagcga cggaccccgt ccggagcgcg
     4981 catcgtcacg ctggatcagg gaaaccccac gctcctctat ctggcccatc ggaagggctg
     5041 gcacgcgagc ccggaggacc tcgcttcgga cacgctcagg gtccgcgcgg cgggcggcgc
     5101 actctacgcg gccggtctcc gtcaggatct ccacggctcg accgcgctcg aaaatggcct
     5161 cgaggcgggg ctcgggcgcg acctcctggc gtccgatccc gacgcattcc tgctcgagct
     5221 tggaccacct cgcgcaggcg gaacctccgg cagatgaatc tcttggattg acttgccccc
     5281 ctccgctcgg gtagattccg gcgttctcat ttcatcgacg cgtatcggct ttgggggtag
     5341 ggcgtgacac cgagcgaccg ccgcaagctg ttcggaacgc tgatcgggct ggccatctgc
     5401 atctgggcgc tctggccgac cttcaccttc tattcgttga gcccggcaga gcgccgggaa
     5461 gtgctccaag cccgtcccgc cgcggccacc aacgacgtcg atcgcgcccg cctcgagaag
     5521 atggccaagc tgcgcgacgg cgcgatcaag ctcggcctcg acctccaggg cggcatgtac
     5581 ctcctcctcg aggtggacaa gtcgaagctg ggcccggccg aggcgaagaa cgccgtcgac
     5641 caggccatgc agatcatccg caaccgaatc gaccagttcg gcgtggcgga gcccaccatc
     5701 cagaagcagg gcgacaaccg gatcctggtc cagctccccg gcctcctcga ccaggggcgc
     5761 gccaaagagc tgatcggcca gaccgcgctc ctcgagttca agatcgtcaa gacggacgac
     5821 gaggcgaagg cgctcttcaa ccggatcgac gcctacttca cgcggaagat gggcggcgcg
     5881 gccgccgatt ccaccggacc ggactccctc cggcatccgt tcaccggccg cttcctcacg
     5941 gtcgcgcagg gctcggagag cttcgcgctc tcgtccgagg tcgccgcgat cgactccatg
     6001 ctcaccgtgc tccggtccga ctccacgttc acgacggacg ccgcgattgc gtgggacgcg
     6061 cacgagacag agctccaggg gcggagcggg cgaaatctct acgtcgtcga caaagagccg
     6121 cagatgaggg ggaccgaggt ggcgagcgcc cagatgcgcc tcgatctcga ccaggagcgt
     6181 ccgggatccc cgggcgtctc cttcaacctc acgagcaagg ggggcgccgt cttcgccgac
     6241 gtcacctcgc gcaacgtgca gcggcggctc gccatcgtcc tcgacaatcg cgtgcagtcg
     6301 gcgccgaaca tccgcgagcg gatcccgggc ggacacggct cgatcacggg ctcgttcacc
     6361 gaggaggagg cgcagaacct ctccatcgtg ctccgctccg gcgcgctccc ggccccggtc
     6421 aacatcgtcg aggagcggag cgtgggcgct tcgctgggtc aggattccat cgagaagggc
     6481 gtccgtgccg gcgcggtcgg cgcgctgctc gtcatcgtgt tcatgatcgt ctactaccgg
     6541 ctctcgggcc tcgtcgccgt ggccgcgctc ctcctcaaca tcatcggcct cctggccgcg
     6601 atggccgggt tccacgcgac gctgaccctg cccggcatcg cgggcatcgt gctcacgatc
     6661 ggcatggccg tggacgcgaa cgtgctcatc ttcgagcgca ttcgcgagga gctccggaac
     6721 aagcgcaccg tgctcaacgc gatcgacatg ggctactcgc gcgcgtggcg caccattctg
     6781 gacgcccacg tgacgacgct cctctcggcg gtcgccctca tgtcgttcgg cacgggtccg
     6841 atccggggat tcgcgatcac gctctcgatc ggtctcatca tcaacatgat caccgccgtc
     6901 ttcgtctcca agatgatctt cgacgcctgg tcgctaaggc ggaaggtcag caccatcagc
     6961 atttagctaa ggagaaccgg tgctcgaaat cctccatggc gtgaacgtcg actggatggg
     7021 caagaggcac atcgcctact ggatctccgg catctgcgtc gccatctcca tcgtctccct
     7081 gatcatgcac ggcggtccgc gctacggcgt cgacttcacg ggcgggaccc tggtcgagct
     7141 ccaggtgacc ccgccgctcc cggtcgacaa cgtccggcgc gcggtggacc aggccggctt
     7201 cacgggctcc gagatccagg cgctcaacgt tcccggccag tacctcgtgc gcatcggtct
     7261 gaccgagctc cacaaagtcg accccgctcc tcgcgtccgc gaggcggtcg ccatcaccaa
     7321 tcccggcgcg acggtcgaga tccggaaggt cgagaccgtg ggcccacgcg tcgggagcga
     7381 gcttcgcggg agcgccctca aggcgatctt cctcgcgctc ggtctgatcc tggtctacgt
     7441 ggcgttccgg tacgactggc gctactcgct cggcgcggtg gtcgcactct tccacgacgt
     7501 tttcatcgcc ctgggcgccg tctcgctcac gaacaaggaa gtcacgctga cgatcgtggc
     7561 cgcgctcctc acgatcgccg gtttctcgat caacgacacc atcgtcgtct tcgaccggat
     7621 ccgggagcgg aagcagacgc tccgccgcga gcatccggcc aaggtcatga atatcgcggt
     7681 caacgagacg ctgagccgga ccatcatcac gtcgttcacc gtcttcctga ccgtgctcgc
     7741 gctctacctc ctcggaggcg aggtgctcca cgacttctcg ttcacgctgc tggtcggcat
     7801 cgtgttcggc acctactcct ccgtctacgt ggccagcggc ctcgccctcg acacccagct
     7861 ctacctcgag ggacaggggc tgcgccgcaa gcaggctccc gccccgacga agaccgcgaa
     7921 gacgcgcgga taagggcgcc gggctccgcc gcgccgcacg cggaacggag gatcgcagca
     7981 tggctcgacc gcgaacgggg ttgggtttcg gcgccgtgac tctcgccgcg gccgcgctcc
     8041 tcgcatctcc tgacgccgcg cggttcgcct cggccgaggg aactcccccg cccgccgaga
     8101 ccgcaccccc ggttcctcgc gtccaggatt cgccgctcaa gctcttcgcc gcggtggaag
     8161 atgcgtggct cgcatcggac gccgagcgcc tggccgatat ggtggacacg acggtcgtgc
     8221 gcatcacgct cgagccgggg accccgccga cgtccgcgat cacgcgcaac gctgcccttt
     8281 tcctcttccg cgaccagctc cgcctcgtga agacgaaggc gtttcgggtg acgcggctcg
     8341 agatcgggca gaagtcgaag ccgaccgcga ccgccaccgc gatctggagc gcggactggg
     8401 gcggccggcg cggcatgcgc gatctcaaag tggcgttcgt cgccgtcccc gtagggagcc
     8461 gctgggtttt gaccgaggtt cgagccaacg actgatcgtt ttccctcaga ctccgtaggt
     8521 cgatcgtgtc cgactcccgc tccgagcaca ccaccgccga cgttctctac gcctccgatc
     8581 tgcacgggca cacgggtctc taccgcgagc tgctcgagac cgcgtggcgg ctccgcgtcc
     8641 gcgcggtgat cctcgggggc gacctcgccc cgcacgcgga cgtcgcgacg cagcggcgct
     8701 tctacgagga gttcctcgtc ccgctccttc gcgactaccg gggcaagccg ggagcggccg
     8761 agatcttcca catccccgga aacgacgact ggaaggcatc cctcacccca ctcctggaag
     8821 cctcgctctc cggcacgcac gcgatccacg gtcgcgtggt ccccttcctc gaccatggct
     8881 tcatcgccgg cctcgcgtcg gttcccatca cgccgtttcc catgaaggac tgggatcggt
     8941 gggaggaggg acttgcggcg gcggccaaga tggagggatt ccgaagcgag ccggacggcg
     9001 gccttcgcgc gtacacgttc cgaggccgcg agcggcgcga atcgctggcc gcggatctcg
     9061 acgcgctgga gcgggaaatg cccgtggacg gcacggagcc tttgatctgc gtcctgcacg
     9121 ggccgccgta cgggaccgcg ctggaccaga tcgcccgcgg agtgcatgtg gggtcgcgcg
     9181 aatcccggcg attcctggag cggcggggtc ccctgctcgg gctccatggc cacatccacg
     9241 agtctccggc gatttcgggt cggttcgccg agcggcttgg ccgtacgatc tgcatcaatg
     9301 ccgggcagcg gatggggagc gcgctccacg cggtttggtt tcgcctggac gatataccgg
     9361 gaacgctgac gcattcgctc ttgggaccgg cccaactggg atgagccgtt cggtctagtc
     9421 cttttggaag ctgtgggcct tgaccgattc ccgattttgt atactgtata caatatggag
     9481 tcgcccatga accgacgacg ctccccagcc gccgcccgca tcgccctttc cctcgctgcc
     9541 gcaacgctag tatcccatgc cacaatcagt tgcagtcagg cccaacccgg ggaggactct
     9601 cgctggtggg gccacgtccg ggcgctggcc tccgatgacc tccggggacg acggaccggg
     9661 accgaggact ttcgccgcgc ggccgcctac gtggaagccg cctaccggga gctcggggtc
     9721 gattcgatgg ggcccgcggg ctatggccag gacgtgccgt tcctggtgcg gacggtcgac
     9781 gagcctagct ccagcgtgac cctctggccc gaggaaggaa gcccgatcgc cctccgcttc
     9841 ggcgacgacg cgaccctcat ccggagcgcg ccgctcgtac gggacgtcga ggcgccgctg
     9901 gtctttgtcg gctacgccgt ggtcgcgccc gaagccaagt acgacgacct cgaatggcag
     9961 gacctgaaag ggaagatcgc ggtcttcgtg agctccggcc ccgccgccgt gccggatcca
    10021 atgcgcgcgc acgtgcagtt cgcggccgag cgctgggccc acctcaaggc cgccggcgcc
    10081 atcggggcca tctccatccc caacccggcc accatggagg cgggatggtc gcgctacatc
    10141 ctgaaccggg cgcagccggc catggcgctg gatgacaagg cggaccatga gctcgatggg
    10201 cagcgcttcg gcatgcggct caatccagca tgcgcggaca cgctcttcaa ggactcccag
    10261 cacacgtggg ccgagatcgc ggccatggcg aagcggggcg atcagctccc caccttcgac
    10321 ctgcccatgc ggctcaaggc ccacgtggag gcgaagacct ggcgcgttcg ttcgcccaac
    10381 gtgatcggcg tgatccatgg atccgatcca acactccgca acgagtacgt gctcctcacg
    10441 gcgcacctcg accacctggg cgtgggcgcg cccgtcgacg gtgactcgat ctacaacggc
    10501 gcgatggaca acgcctcggg ggtggcgacc cttctcgagg tggctcgggc cctgcgccag
    10561 ggctccgcgc ccaagcgctc ggtgatcctc gcggcgctca cgggcgagga ggaagggctc
    10621 tacggctcgc gctacttcgc gctccatccg ctcgtgccca tgtcgcggat cgtcgcggag
    10681 atcaacgtcg acatggtgct ccccatcgtt ccgctcacgc acatcgtcgt gttcggcctc
    10741 aacgagtcgt cgctcggcct ccgcgcgacc gaggcggccc agcgccacgg cgttgcgacc
    10801 cagaaggacc ccgagccgca gcgcaatctc ttcatccgga gcgatcagta caacctgatc
    10861 cgcgccggcg ttccgtcgct cgcgttcgtg acggggttcg tgccggactc gccggccgag
    10921 aagcagctca aagagtggcg tcacaatcgg tatcacgcac cgtcggacga tctggaccag
    10981 ccgatcgacc accaggcggc cgcgctctgg aacgagatcc tcctcgacct cgtgcgggac
    11041 accgccgacc gcgcagagaa gccgcgctgg aattccgaca gcttcttcgc gcgcttcgcg
    11101 aaggagcccg cgaccaactc gccaagagcc gcccggccgt gatcacgatc gcgcgcgcgc
    11161 cgctccggcg cgaggtgcgc cgcatcgtgc tggatcggat cctgcgcggc gagattccgc
    11221 ccgggggcgg gatcaacgaa tcggagctcg cgatcgacat cggcgtgagc cgcacaccgc
    11281 tccgggaagc gctcctcggc ctggagcgcg agggatttct tcgctccgag acggggcgcg
    11341 gcttcttcgc ggcggcgctc actgccgagg acgcggagga gctctatccc atcctctgga
    11401 cgctcgaggg gcttgcgctc gcgtcgagcc cgtcgccgac tcccgcgcgc aaggccgagc
    11461 tcgaacgcat caacgcggac ctcgcgcgct acgagcggat tcccgagcgc gcgctcgatt
    11521 tcgaccgcct gtggcacgac acgctcctcg ccgcgtgccc gaagcggagg ctcctcgcga
    11581 tgaccgaggt gctgaaggac caggcgcgcc gctacgagcg cgcgtacatg gccgactccg
    11641 ggcgggtgtt cgtctcgacc cggcagcacg aggagatcct cgaggccctc gagaaagacg
    11701 agcgcgagag agcggtccgg ctcctcgaga ccaactggcg cgtgagcctc gacttcctct
    11761 tgccgtggct ccggaaggcg cgcccttgag cgcgcgtgcg gccgcagcgc gggaaccgct
    11821 cgcgcccgag ttcgtcctcg agattctcca gagtctcgtg cgcatcccgt ccgtgaatcc
    11881 gacgctcgct cccgaggaag gcggaggaga ggccgagatc gccggcttcg tgcgggactg
    11941 gctcgacgcg cgcggcatcg aagcgcgcct cgaggaagcc gccccgggac ggcccaacgt
    12001 gttcgcggcg gtcggagcgg atgcgggccc gaccctcgtt ctgtgcgcgc atctcgatac
    12061 ggtcgggacc gcggggatga cgattccccc gttcgagccg cgcgtggaag ggaatcgcgt
    12121 gtatgggcgc gggagcttcg acatgaaggg aggcgtggcg gcgatcctcg cggccgcggt
    12181 cgcgcttcgg gacgccggtc tccaagggcg cgtgctcctc gcactcgtgg cggatgaaga
    12241 gtacacgagc ctgggagcgg acgacttcgt gcgacgccat cgcgccgatg gttgcatcct
    12301 gaccgaagcg agcgaggggc gcctgatcac cgcgaccaag ggattcgcgt ggatcgaagc
    12361 gcgtacggag gggcgcgcgg cgcacggcag ccgatgggat atcggcgtga gcgcgatcgc
    12421 gcggatgggg cggatcgtgg ccgctctcga cgacctcgat cgccgcgtcc tccgccagcg
    12481 gacccatcca ctcgtgggtc ccgcctcgct ccactgcgcc aaaatcgacg gcggcgtggg
    12541 gctttcgacc tatgctcccg catgcacgct cacggtggag cgccgcacgc ttcccggcga
    12601 gacccccgaa agcgcgctcg aggagatccg gtcgaccatc gcgggcgccg gcgaggaggc
    12661 gaacctgtcg atcgttttcg cccggccccc tctgacctgc gatcccaggg ccaaggtcgc
    12721 ccgcgcggcg cgcgaggc
//
LOCUS       DASTKC010000272         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_708288, whole genome shotgun sequence.
ACCESSION   DASTKC010000272 DASTKC010000000
VERSION     DASTKC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708288"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VFR25_11280"
     CDS             <1..901
                     /locus_tag="VFR25_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941671.1"
                     /translation="DAVQFVLMYLGFLIALPLLVARYGGLSFLRAHLPATHFVWHGGN
                     APQYIFVWYLIALQTLVEPTFYQRAFAAVDEKVARRGVLISIAFWMLFDFLTTATGLY
                     ARALLPNLKDPVAAYPALAVDILPPVVRGLFYLGLLATVMSTVDGYTFIGGVNFGRDL
                     IWRWRGDKDESRVNRYVQVGFVVTAIVSILLALFVRSAVDLWHDVGSVGVPALLIPLL
                     ASYSERFRLPPRAAVASMLAGAGVALLWLLSRYFAWTPGYPLGIEAIYPGLLASLIPA
                     LWGHLQGRKKSPPKGNLSPGPVI"
     gene            1108..2670
                     /locus_tag="VFR25_11285"
     CDS             1108..2670
                     /locus_tag="VFR25_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941672.1"
                     /translation="MRYTPAMAHAQGTSKTLPWRAAALWSLLALVLATLFACVTFSRV
                     PPSSDVWDYAQEARQLARGQGFTSLYTYPTHLGKEPAPFPVRWRMPLYAAIGAAFLTL
                     GLPLPLGFFIVAVLAHAVLVGLVFLLASHLHSPRAGVLAAAFAIASPILLDPFSAGLS
                     QVPTAALGAAIWLLLLRYARPSSAAAAGVLAAAAWYLRGETIVMLPLWFACAARGRRW
                     DRAMAFALVFAALALPWIGHLRAETGAATPIHGNPMLLYTPEYPGYSSTRTYLEPMPG
                     VVAYVATHPLTFAWRFTKDAVGYGVDLLWGFGPFVLGLAMAGLLLRDPNRRWSVLRPA
                     AWILVAALVQIAAFSCLERSPRFLVPAIPLIAVALGMAAAPTFDRICGRNRQAVLIAL
                     LVLERAILLGITARDAERRFPPLPPSTATQLRDALGPARGGIIWTDVPDWVSWHLDRP
                     ALLLPLWRQLNRVAADHPADAIYLSPGARSRNEADGEARWIRAADGLDSIPGFSKPDA
                     LSAGGQVFLPIY"
     gene            complement(2695..3369)
                     /locus_tag="VFR25_11290"
     CDS             complement(2695..3369)
                     /locus_tag="VFR25_11290"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HET9941673.1"
                     /translation="MSKLSAAPVHRVAIALLALCLVSPAIASDKSSKKTPKTPAASST
                     AAADKAAADAKAQQDAMMAEMAKYAAPGPMQTDFMKPLTGTWKTVTTTYMGPKPETSE
                     GTCTRSMVLGDRFLESKHSGVFAGAPFEGMELLGFDTRKNQFSSVWIDNMGTSMAVST
                     SGSYDAATKTLTINSTFDDPVSGKPMAYKMVTHVVDPNKHVFTMVENKDGKDVTDMEI
                     TYTRMQ"
     gene            complement(3500..3853)
                     /locus_tag="VFR25_11295"
     CDS             complement(3500..3853)
                     /locus_tag="VFR25_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9941674.1"
                     /translation="MTRTHVLASVAFGMLLPVAAFAGTGTTTTASTAHHSSSAHHNSK
                     TASHKVDLNTASKEDLMKLPGVDDATADKIIAGRPYTSKSELVKKNVLTQEEMKKIDA
                     HVMAKAPAKEAAAKK"
     gene            complement(4603..>5146)
                     /locus_tag="VFR25_11300"
     CDS             complement(4603..>5146)
                     /locus_tag="VFR25_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HET9941675.1"
                     /translation="EKGFCMGRFDPEHLARSWVSVAWHPGERRAAAFLTWNEVPARDG
                     WALDLMRRRREAPNGTMEFLIAKSVEAARDRGDDMLSLSLSALAKVDPDQGTPAPPAA
                     EGADRLRVFLLDHLSRFYDFQGLFHWKKKFAPEFEDRYLVYGEPLSLPRVVLALARAQ
                     SPGGLLSYLRGPGVPAALSS"
BASE COUNT          725 a         1747 c         1707 g          967 t
ORIGIN      
        1 ggacgcggtg cagttcgtgc tcatgtactt gggctttctc atcgcgctgc cgcttctcgt
       61 ggcgcggtat ggagggctct ccttcctcag agcgcacctg cccgcgaccc acttcgtgtg
      121 gcacggcggg aatgcgcccc agtacatctt cgtctggtat ctgatcgcgc tgcagacgct
      181 ggtggagccg accttctacc agcgtgcttt tgccgccgtg gacgaaaagg tcgcgcggcg
      241 tggcgtgctg atctcgatcg cgttctggat gctgttcgat ttcctcacca cggcgacggg
      301 actctacgct cgggcgcttc ttccgaatct caaggatccg gtcgccgcat atcccgcgct
      361 cgctgtggac atcctgcctc ccgtggtgcg tggactcttc tacctcggcc ttctcgcgac
      421 ggtgatgtcc acggtggacg ggtacacgtt catcggcggc gtgaacttcg gccgcgatct
      481 catctggcgg tggcgcgggg acaaggacga gtcgcgcgtg aatcgctacg tgcaggtggg
      541 attcgtggtg accgcgatcg tgtcgatcct gctcgcgctc ttcgtgcgct ccgccgtgga
      601 tttgtggcac gacgtgggat cggtcggcgt gcccgcgctc ctgatcccgc tcctcgcgtc
      661 gtacagcgag cgcttccgac ttcctccgcg cgctgccgtg gcatccatgc tggccggagc
      721 gggagtggcg ctcctctggc tcctgtcgcg ctatttcgcg tggacccccg gatatccctt
      781 ggggatcgag gccatctacc caggcctcct ggccagcctt atccccgctc tgtggggcca
      841 cttacagggg cgcaaaaaaa gccctcccaa agggaacctc agtcccgggc cggtgatcta
      901 aatagatgag agtggtgggt taaccaccga cgattcagcc ggttagctcc ttggttgcct
      961 cctagcaacg agcgccggct gatcggaact ctcatgagga accgatccgc ggacccccca
     1021 cagacccgcg gacctcacgg aatctgcgga acgcaagacc gcagaccctt gccccgccgt
     1081 tggaacccgg cggggcactt tttttcggtg cgctacactc ccgccatggc gcacgcgcag
     1141 ggtacctcca agacgcttcc ctggcgcgcc gccgccttgt ggtcccttct tgcgctcgtg
     1201 ctcgcgacgc tcttcgcctg cgtcacgttc tcgcgcgtcc ctccgtcgag cgatgtctgg
     1261 gattacgcgc aggaagcgcg gcagctcgcg cgcggccagg gcttcacgtc gctctacacg
     1321 tatcccacgc atctcggcaa ggagccggct ccgtttcccg tgcggtggcg gatgcccttg
     1381 tacgcggcca tcggcgccgc gttcctcacg ctcggcctac cgcttccact cgggttcttc
     1441 atcgtggccg tgctcgcgca cgcggtgctc gtcggcctcg tcttcctcct cgcgtcgcat
     1501 ctccattcgc cgcgcgccgg ggtgttggcc gcggcgttcg cgatcgcgtc gcccatcctg
     1561 ctcgatccct tcagtgccgg gctgagccag gtgcccacgg cagccctcgg tgcggcgatc
     1621 tggctgctcc tgcttcggta cgcgcgcccg tcctccgccg ccgccgcggg agtcctggcc
     1681 gcggccgcgt ggtatctgcg cggcgaaacg atcgtgatgc tcccgctctg gttcgcgtgc
     1741 gccgcgcggg gcagacgctg ggatcgcgcg atggcgttcg cgctcgtctt cgccgcgctc
     1801 gccttgcctt ggatcgggca tcttcgcgcc gagaccggag cagccacgcc catccacggc
     1861 aatccgatgc tcctctacac ccccgagtat cccgggtact cctcgacgcg gacgtacctc
     1921 gagcccatgc ccggcgtggt cgcgtacgtc gccacgcatc ccctcacatt cgcgtggcga
     1981 ttcaccaagg atgcggtggg atacggcgtc gatcttctct ggggattcgg gccgttcgtg
     2041 ctggggctcg cgatggcggg acttctcctg cgcgatccca accgccgatg gagtgtgctt
     2101 cgacccgccg cgtggatcct cgtcgcggcc ctagtccaga tcgctgcgtt ctcctgcctg
     2161 gagcggagcc cacgctttct cgtgccggcg attccgctca tcgccgtcgc gctcggcatg
     2221 gcggctgcgc cgacgttcga tcgcatctgc ggccggaacc gccaggccgt gctgatcgca
     2281 ctgctcgtcc tcgagcgcgc gatcctcctg gggatcaccg cgcgagacgc cgagcgccgc
     2341 ttcccgcctc ttccaccctc caccgccacg cagcttcgcg acgcgctcgg ccccgcgcgc
     2401 ggcggcatca tctggaccga cgtgcccgac tgggtgtcgt ggcacttgga ccgccccgcg
     2461 ctcctcctcc cgctctggcg gcagctaaac cgcgtggccg cggatcatcc cgcggacgcg
     2521 atctatctct cgcctggcgc gcggtcgcga aacgaggccg acggcgaagc gcggtggatt
     2581 cgcgctgccg acggcctcga ttcgattcct ggattttcca agcccgacgc tttgagcgcc
     2641 gggggccagg tcttcctgcc gatctactga ccgcgcctca cagcgcggac gcgcctactg
     2701 catgcgcgtg taggtgatct ccatgtccgt gacgtccttg ccgtccttgt tctcgaccat
     2761 cgtgaagacg tgcttattgg gatcgacgac gtgggtcacc atcttgtacg ccatcggctt
     2821 cccgctcacc ggatcgtcga aggtcgagtt gatggtgagc gtcttcgtgg cggcatcgta
     2881 ggacccgctc gtgctcaccg ccatggacgt tcccatgttg tcgatccaga cgctcgagaa
     2941 ctgattcttc cgcgtgtcga agccgaggag ctccatccct tcgaacggcg cacccgcgaa
     3001 gacgcccgag tgcttgctct cgaggaaccg gtcaccgagg accatgctcc gcgtgcacgt
     3061 tccctcggag gtctccggct tcgggcccat gtaggtggtc gttacggtct tccacgttcc
     3121 cgtgagcggc ttcatgaagt ccgtctgcat cgggcccggc gccgcgtact tggccatctc
     3181 ggccatcatc gcgtcctgct gggcttttgc gtcggcagcc gccttgtccg ctgcggcggt
     3241 cgaggatgcc gcgggtgtct tcggggtctt cttggacgac ttgtcggatg cgatggcggg
     3301 tgagacgaga cagagggcga gcagtgcgat ggcgacccgg tggaccggag cggcggagag
     3361 cttggacatc gtgtcctcca cgttctagga gatcaggggg tgtctcactg agaccgtgaa
     3421 aagtagcacg agccacgccg cccggccacc ggttcgctta ggggcggcgg cgcctcccgc
     3481 cgccccacgc ttcaaccagc tacttcttgg cagcggcctc cttggccggc gccttcgcca
     3541 tcacgtgcgc gtcgatcttc ttcatctctt cctgcgtgag gacgttcttc ttcacgagct
     3601 cgctcttcga ggtgtacgga cggccggcga tgatcttgtc cgcggtcgcg tcgtccacgc
     3661 ccggcagctt catgaggtct tccttcgacg ccgtgttgag atcgaccttg tgcgaggccg
     3721 tcttcgagtt gtgatgcgcc gacgacgagt ggtgcgcggt gctcgccgtg gtggtggtgc
     3781 cggtgccggc aaaggcagcc accggcagca gcatgccgaa cgcgacggag gccaacacat
     3841 gggtacgggt catgactttt ctcctgtgga gggggacatg agccgcgccg gagagccctc
     3901 cacaccggcg gcgcgagagg gcggagccgc cctcggggca aagcaaatgg ggttacggga
     3961 ggtggtgctg gatgtagtgg tggaacggac ggaagcgaac agcgaagcgg atgggatggc
     4021 tggggtcgga gcggggcaag gccgcatgct gcgatccgag ctgccttctt ggatcgcgga
     4081 gagcaacgat gggcctgcgc gcgggggacc gcgtcccgaa atcaccgaac catccctatg
     4141 cttgaacgac aacgagcgga agaaaccgat acgccaggcg gcggcgaggg cgggagccgt
     4201 cggctccagg cccaaggcgg ccgcgtgagc attcactctg gggcgcttga cgaggcgcgg
     4261 gtgcgaggtc gtgcgggcag cgcgagaagg gtgttggggt ccgggtcggc gcgcgggctg
     4321 aggatgtggg tacgtggagc ggctcgcggc catgccagga tatgcggtaa ccgggcggag
     4381 ctggcgagcg gtgtctctgg gaggattcat tgcggcgctc gccggcagcg gacgggccag
     4441 gatggcgagg actccgatcg ccattcctag taccaagagc cgcaacggct tcaatgccac
     4501 accctccctc ttgcgttgga gccgacccgg ccccgtgtgc aatcggggat acacccgccc
     4561 cacgggtcgg ttcctgaact tcgggaccat agtactcccg gctcaggagg aaagggcagc
     4621 cggaacgcca ggcccccgga ggtagctcaa gagcccgccc gggctctggg cccgggccag
     4681 cgccaggacc acccggggga gcgaaagcgg ctctccatac acgagatagc ggtcctcgaa
     4741 ctccggggcg aacttcttct tccagtggaa gagcccctgg aagtcgtaga accgggagag
     4801 gtggtcgaga aggaacacac gcagccggtc cgccccctcg gcggccggcg gggcgggcgt
     4861 gccctggtcg ggatcgacct tcgcgagcgc tgagagggag agcgagagca tgtcgtcgcc
     4921 gcgatcgcgc gccgcctcca ccgacttcgc gatgaggaac tccatggttc cgttcggcgc
     4981 ctcgcgccgg cggcgcatga ggtcgagcgc ccagccatcg cgcgcgggga cctcgttcca
     5041 tgtgaggaac gccgccgcgc gacgctcgcc cggatgccac gccacgctca cccacgagcg
     5101 tgcgaggtgc tccgggtcga agcgtcccat gcaaaagccc ttctcc
//
LOCUS       DASTKC010000273         8662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1922 k141_708335, whole genome shotgun sequence.
ACCESSION   DASTKC010000273 DASTKC010000000
VERSION     DASTKC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37969998
            Sequence Read Archive: SRR25158375
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 21:03:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,269
            CDSs (total)                      :: 2,238
            Genes (coding)                    :: 2,227
            CDSs (with protein)               :: 2,227
            Genes (RNA)                       :: 31
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 0 of 11
            Pseudo Genes (incomplete)         :: 10 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708335"
                     /isolate="SMAG_U1922"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="29.58472222 N 107.6005556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..521
                     /locus_tag="VFR25_11305"
     CDS             144..521
                     /locus_tag="VFR25_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9941676.1"
                     /translation="MIGDKEAAATIAVKDLAVAKDFYETKLGLKRVGTEGDEAVVYRS
                     GVSKVVVYRSEYAGTNRATAANWMVGKDLEREVETLKGRGVRFEHYDMPGLTRQGDVH
                     VAGDMKVAWFKDPDGNILSLVSG"
     gene            complement(559..4491)
                     /locus_tag="VFR25_11310"
     CDS             complement(559..4491)
                     /locus_tag="VFR25_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9941677.1"
                     /translation="MIGKKLRAYEITEEIGAGGMATVFRAYQPSMDRHVAIKVIRTSI
                     LHDEGLRERFQREARLIARLEHPHLLPVYDFDGESDPPYIVMRYLEGGTLKQVQQRGT
                     VPREEILHVLRQLAGALDYAHRQGIVHRDLKPSNIMIDREGNAFLTDFGIARSTGKDK
                     DLTGTGLMIGTPGYMAPEQARGDGQAGKAADIYSLGVLAFEALAGSAPYEHESGFEVI
                     LAHMNAPIPKASERNRDLPKAVDTVLARALAKQPSERFSSATEFVEALTRALKIKPSD
                     EPTRLKSMTQTISVDQLAKLAASKGERETPSTGTPSDQQRQMTALAVDVKELAEILYE
                     SGADNERVRATVSALWTAFGEIAKEASGIVHSRTEDAGLLLWGRERAREDDPENAIRA
                     ALAMRDHALAESRKVLGSSWEPTEDNPLPFAAGITTGPVLLERDGDSGSYTASGAPIT
                     LAARLKEAAPPGGILAAHETYSLVRGVFNFLQQDAVRVRGRREPIEVYVVTSAKPRAF
                     RLRSMGIEGVETKMIGREIELRLLQEAMSLTLEDGETQVVTVVGEAGVGKSRLLYEFN
                     NWIDLQEQVVWFFEARATQPSMLQPYSLARDLFSFRFRILDSDPINQLHEKFVQGVAG
                     FLGEGSETKAHYIGQLVGFDFSGIPEVAAALKDGEAFRRTAQTYLGELFTTASRTNPV
                     MLLIEDIHWADDRSLDLINNLVRDNTDLPLFVLCMSRPSLYERRPQWGEGQRFHERIQ
                     LEPLSQLSSRRLVRELLKHVPEVPTALRDLVVDRADGNPFYIEELIKALIDDRVIVKG
                     DPNWSVDTTRLSTVRVPATLTGVLQARLDTLPPALYQLLQRASVIGRIFWDAAAIRLS
                     QESAGLGADEIQAMLEDLRGREMILQREESGFAGTVEYVFRHAILRDVTYETVIPRQR
                     RALHKMVGDWLIEVGGERADEHTLLVAEHYSRAEEASLAAAQLIKVGERGIRLAMYDE
                     AATVLQRAREMLASTEYAEQRLLVDSLRGDIETFRASYSKATEIFRPILEEARAIGSL
                     PVQAQALGKLGRLAMWQQDVPQALSYLDEAITISRAIGDEATLMFNLRQVGNVLHESD
                     PERAIQHLEESATIARKLGDRASEASALNSLGIVLFVVRRVDEARETYQRAVAATRAV
                     GDRLGEAMSRGNLGGLYADQNDPDAERYLQEALSMSRELRVPALVVDQGTGLAAFYLR
                     QERDREAYPLLKDAVRAAREIGLPLYNFPFLYGVIRLHAGDRAKGLAWIGCSRAKSPQ
                     VFEINMVMRWFHDVIRGNDSVEEVERAMRAGEELKVEDILAEAERMAP"
     gene            complement(4612..4983)
                     /locus_tag="VFR25_11315"
     CDS             complement(4612..4983)
                     /locus_tag="VFR25_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9941678.1"
                     /translation="MTTSLKERAVSFLKLAAAGRLDEAYDLVGTGFLHHNPYFRGDAP
                     SLKAAMAENAAQNPRKTLEVKRALEDDDLVAVHSHVRQKPEDPGAAVVHIFRFEKGRI
                     VELWDVGQPVMDEGPNEHGMF"
     gene            5124..5588
                     /locus_tag="VFR25_11320"
     CDS             5124..5588
                     /locus_tag="VFR25_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9941679.1"
                     /translation="METFLPIREVAARTGVSVHTLRYYERAGLLPRAGRSAAGYRLYT
                     AESVRRVMLVRTLRSVGFGIKELRGLGAVTDLRIPTATMRARLRAKRDQVSGQLRDLE
                     RAWKLLDALQGCRCRGDCVLVTRLLDRGESKLAVTRRSSSVRTRPKRRRMNR"
     gene            5585..6037
                     /locus_tag="VFR25_11325"
     CDS             5585..6037
                     /locus_tag="VFR25_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9941680.1"
                     /translation="MTSRAKLPRFVLVLAWTLATRLFAPSSGLAADAIKTPAPQGHES
                     PFACIMSALSPAERKQHFEEYGPRLRARLESIRELPNGYEFAFHTDKDTYQVLSTWMF
                     QERLCCPFFDLELRIDREGGSTWLRLTGREGVKDFIQAEFEPWFRKGS"
     gene            6096..6905
                     /locus_tag="VFR25_11330"
     CDS             6096..6905
                     /locus_tag="VFR25_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9941681.1"
                     /translation="MSAPGIVRIGLASVRNVPSESGRLDTLRRVLDEARAQGVAIVCF
                     PETYLFGLRGQDFPVAPPDQGRQESALEEVSRAARQTGVAVVVPMEWQTRAGLHNVAF
                     VISSVGECLGFQPKNQIPVEEEPHYAPEGSRRMFEVDGLTFGVTICHEGWRYPEATRW
                     AAARGAHVVFHPQLTGGDDKGVTPSFWGDPKAPYYEKAMITRAVENTVYFASVNYAMR
                     HQESATSLIDPDGVCVAHVPYGEERLLVHDVDPARATGFCARRYRPELYPA"
     gene            6902..7372
                     /locus_tag="VFR25_11335"
     CDS             6902..7372
                     /locus_tag="VFR25_11335"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET9941682.1"
                     /translation="MKPLPPPSADLASAILGSWWLLSRLDHDDGGNRHVCPILGAEPL
                     GYLSLTPRRFSAQFMRRGRELEAEPLRAPATTAAANNTGAVGGYDAYFGTYELDAGRG
                     TVVVTLEAALTPANIGKTFLREIRVVDGRLIIRLNTDAADGTPISRTLTFERLA"
     gene            complement(7378..8067)
                     /locus_tag="VFR25_11340"
     CDS             complement(7378..8067)
                     /locus_tag="VFR25_11340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9941683.1"
                     /translation="MTEPRRPLIASLRHTAILTAILLGVAAYGIYVQSQGAERSAGNR
                     ASALPLYLSLLAAEWGLVRYVMVGLRRTGTRVRDLVGARWSRPLDVLRDVGIGLAFWA
                     AWALVASLLVRVVGPDPKSVEALLPRDLPESVVWVLLSLSAGFCEEVVFRGYFQTQLR
                     ALTGHAVPAILGQAVIFGITHGYQGIRNVIVITGLGVLFGLLAHWRRSLAPGIAAHAW
                     TDIVAGLMMRG"
     gene            complement(8064..>8662)
                     /locus_tag="VFR25_11345"
     CDS             complement(8064..>8662)
                     /locus_tag="VFR25_11345"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9941684.1"
                     /translation="LDISFRYMRAGSSTTPYAVLGLLQIRPMSGYDLRKEAATSVGFF
                     WSESYGQLYPALKSLAAAGLIRRRPGGATGGRERQVYEITRKGREALACWRTEPPRQA
                     PVRSELLLKLFLGDRASAGPEIAWIDELAAREAAALREFARIRRELERQPDHPSLPFW
                     LITLSYGEHRSRAVRRWCAETRRTLHALARTRSRRKPR"
BASE COUNT         1405 a         2821 c         2967 g         1469 t
ORIGIN      
        1 ccgggcgatg tcgaggccac ggaggcgctc cgggcgctgg ggcggcgcta gctacttttc
       61 ccggacacgc aggctcgagg ggcgtctacc atcaaaagat gccgcgccaa cggctgacga
      121 gcagtgcgtg cgaaggagga gcgatgatcg gtgacaagga ggccgccgcg acgatcgcgg
      181 tcaaggactt ggcggtcgcc aaggatttct acgagacgaa gctcggtttg aagcgagtgg
      241 gcaccgaggg ggacgaagct gtggtgtacc ggagtggagt ctccaaggtc gtggtgtatc
      301 ggtcggagta cgcgggcacc aatcgggcga ccgcggccaa ctggatggtc gggaaggatc
      361 tcgagcgcga ggtcgagacg ctgaaggggc ggggcgttcg attcgagcac tacgacatgc
      421 cgggactcac gcgccaggga gacgtccacg tggccggcga catgaaggtg gcttggttca
      481 aggatccgga cgggaacatc ttgagcctgg tcagcggcta ggcaagctcg caacgctgcg
      541 gaggtgggag taggggtgct acggcgccat ccgctccgcc tccgccagga tatcttcgac
      601 cttcagctct tcccccgcac gcatggcgcg ctccacctcc tccaccgaat cgttcccgcg
      661 aatcacatca tggaaccatc gcatcaccat gttgatctcg aacacttgcg ggctctttgc
      721 ccggctgcat ccgatccagg cgagtccctt ggcgcgatcc ccagcatgga gccggatgac
      781 gccgtagagg aacgggaagt tgtagagggg caggccaatc tcacgcgcgg cccgtacggc
      841 gtccttgagg agcgggtagg cctcccgatc gcgctcttgg cgcaggtaaa aggcggcgag
      901 gcccgtacct tggtccacga cgagggctgg gacccgtagt tccctcgaca tggacagcgc
      961 ctcctggaga tatcgctccg cgtcgggatc gttctggtcc gcgtaaagtc cgcccagatt
     1021 gccacgggac atggcctcac ccagacggtc ccccacggcc ctggtggccg cgacggcgcg
     1081 ttggtaggtc tcccgcgcct cgtcgactct gcggacgacg aacaggacga ttccgaggga
     1141 gttgagcgct gaggcctcgc tggcccggtc tccgagtttc cgcgcgatcg tggcgctttc
     1201 ctccaggtgc tggatcgcgc gttccgggtc gctctcgtgg agcacgttcc cgacctgacg
     1261 cagattgaac atgagcgtgg cttcatcgcc gatggcacga gagatcgtga tcgcctcgtc
     1321 caggtagctc aaggcctgtg ggacgtcctg ctgccacatg gcgagccggc cgagcttccc
     1381 gagagcctgg gcttgcacgg ggaggcttcc tatcgccctg gcctcctcga gaatcggcct
     1441 gaagatctcg gtggccttgg agtaggaggc tcgaaacgtc tcgatgtccc cgcggaggga
     1501 atcgaccagg agacgctgct cggcatactc ggtcgaggcg agcatctccc gcgctctctg
     1561 caatacggtc gcagcctcgt cgtacatcgc gagacggatc ccgcgctccc cgaccttgat
     1621 gagctgagcc gcggcgagag acgcctcctc cgcgcggctg tagtgctcgg ccacgagcag
     1681 cgtgtgctcg tccgccctct cccctccgac ctcgatcagc cagtcgccga ccatcttgtg
     1741 gagggcgcgg cgttggcgcg ggatcaccgt ttcgtaggtc acgtcccgca agatggcgtg
     1801 gcggaacacg tattccaccg tgccggcgaa gcccgattct tcgcgctgga ggatcatctc
     1861 gcgccctcga aggtcctcga gcatggcctg gatctcgtcg gcgccgaggc cggccgactc
     1921 ctggctcagg cggatggcgg ccgcatccca gaagatgcgc ccgatgaccg aagcccgctg
     1981 gaggagctga tagagcgcgg ggggcagggt gtcgaggcgt gcctggagca cgcccgtgag
     2041 ggtcgcgggg acgcgcaccg tcgagagccg cgtcgtgtcg acggaccagt tcgggtcccc
     2101 cttcacgatc acgcggtcgt cgatcagcgc cttgatcagc tcctcgatgt agaaaggatt
     2161 gccgtccgcg cggtccacga cgagatcgcg aagcgcggtt ggcacttcgg gtacgtgctt
     2221 caagagctcg cgcacgagac ggcggctcga gagctgcgat aaaggctcga gctggatccg
     2281 ctcgtggaac cgctggcctt cgccccactg gggccggcgc tcgtagagac tggggcggga
     2341 catgcagagc acgaagagcg gaagatccgt gttgtcgcga accaggttgt tgatgagatc
     2401 gagggaccgg tcgtccgccc agtggatgtc ctcgatcagg agcatcaccg gattggtcct
     2461 ggatgccgtc gtgaagagct cgcccaggta ggtctgcgcg gtgcggcgaa acgcctcgcc
     2521 atccttgagg gcggccgcca cctcggggat ccccgaaaaa tcgaagccga cgagctgccc
     2581 gatgtagtgg gccttcgtct ccgacccctc tcccaggaat cccgcgacgc cctggacgaa
     2641 cttctcgtgc agctggttga tcgggtccga gtcgaggatc cggaaccgga acgagaagag
     2701 gtcccgagcc agggagtagg gctggagcat ggacggctgc gtcgcacgtg cctcgaagaa
     2761 ccagaccacc tgttcctgga gatcgatcca gttgttgaac tcgtagagga gacgcgactt
     2821 tccgacaccg gcttcgccga cgaccgtgac cacctgcgtt tccccgtcct cgagggtgag
     2881 cgacatcgcc tcctggagca ggcgcagctc gatctcgcgg ccgatcatct tggtctcgac
     2941 gccctcgatg cccatcgaac gaaggcgaaa cgcgcgtggc ttggcgctgg tgacgacgta
     3001 gacctcgatc ggttcccttc gcccgcgcac gcggacggcg tcctgctgga ggaaattgaa
     3061 gacgccgcgc acgagcgaat acgtctcgtg ggcggcgagg atcccgcccg gaggcgcggc
     3121 ctccttgagg cgcgcggcca gagtgatcgg cgctccgctc gcggtgtagc tgccgctgtc
     3181 cccgtcccgc tcgaggagca cgggtcccgt ggtgatcccc gcggcgaagg gcagaggatt
     3241 gtcctcggtc ggctcccacg acgagccgag aaccttgcgc gactccgcga gtgcgtggtc
     3301 ccgcatcgcc agtgctgctc ggatcgcatt ctccgggtca tcctcgcgcg cgcgctcgcg
     3361 gccccagagc aggagcccgg cgtcttccgt ccgactgtgg acgatcccgc tcgcctcttt
     3421 cgcgatctcg ccgaaggcgg tccagagagc gctcaccgtc gccctcacgc gctcgttatc
     3481 ggcgcctgac tcgtagagga tctccgcaag ctccttcacg tccaccgcca gagcggtcat
     3541 ctgtcgttgc tggtcggacg gcgtgcccgt gctcggcgtc tcgcgctctc ctttggaggc
     3601 ggcgagcttg gcgagctggt ccacggagat cgtctgcgtc attgacttga gccgcgtcgg
     3661 ctcgtcgctc ggcttgatct tgagcgcgcg ggtgagcgcc tccacgaact cggtcgcgga
     3721 ggagaagcgc tcggacggct gcttggcgag cgcccgcgcg agcaccgtgt ccacggcctt
     3781 cggcaggtcg cgattccgct cgctggcctt cgggatcggc gcgttcatgt gggcgaggat
     3841 cacctcgaac ccgctctcgt gctcgtacgg cgcggagccg gccagcgctt cgaacgcgag
     3901 cacgccgagc gagtagatgt cggccgcctt gccggcctgg ccgtcaccgc gcgcctgctc
     3961 cggcgccatg tagccgggcg tgccgatcat gagaccggtg ccggtcaggt ccttgtcctt
     4021 gccggtggag cgggcgatcc caaagtccgt gaggaacgcg ttcccctcgc ggtcgatcat
     4081 gatgttcgag ggcttgagat cccggtgcac gatgccctgg cggtgcgcgt agtcgagcgc
     4141 gccggcgagc tggcgaagca cgtgcaggat ctcctcgcgc ggcacggttc cacgctgctg
     4201 cacctgcttc agcgtcccgc cctcgagata gcgcatgacg atgtacgggg gatcgctttc
     4261 cccgtcgaag tcgtagacgg ggaggaggtg cgggtgctcc agccgggcga tgagccttgc
     4321 ctcgcgctgg aagcgctcgc ggagcccttc gtcatggagg atggacgtgc ggatgacctt
     4381 gattgcgacg tgccggtcca tgctgggctg gtaggcgcgg aagacggtgg ccatgccgcc
     4441 cgcgccgatc tcttcggtga tctcgtaggc gcgcagcttc ttgccgatca tcggatgggg
     4501 acgtccttga gataggggtc gaggttgttg gtgtggggct attgcgccgc aagaggatag
     4561 cggattttcg acgtcggcgc caggggatag gcgaggcggc aaggcggcat ctcagaacat
     4621 gccgtgctcg ttcggcccct catccatgac gggctgtccc acatcccaca gctccacgat
     4681 gcggcccttc tcgaatcgaa agatgtggac cacggcggcg cctggatctt ccggcttctg
     4741 ccgcacgtgg gagtggacgg ccacgagatc gtcgtcctcc agagcccgct tcacctccag
     4801 agtcttccgg gggttctgag ccgcgttctc agccatcgcc gctttgaggc tgggggcgtc
     4861 tccacggaag tagggattgt ggtgaaggaa gccggtgccc acgaggtcgt aggcttcgtc
     4921 caagcggccc gcggccgcca gtttgaggaa ggacacggcc cgctccttga gcgacgtggt
     4981 cacgttcatg gaaaccccct gtcgcaacga gtgctggatg gcttagctcc tgaaatcgac
     5041 gctagacccg ccccgacgcg accgcaagaa ttcccttgga ccttcgagcg cgctcgaacc
     5101 gtaccgtcct ccgtggaggg ctgatggaga cgttccttcc cattcgtgaa gtcgccgccc
     5161 gtacgggagt cagtgtccac acgcttcgct actacgagcg cgcggggctc ctgccccgag
     5221 caggccggtc ggcggcgggc taccggctct acacggccga atcggttcgc cgcgtgatgc
     5281 tggtccgaac gctgcggtcg gtcggcttcg gaatcaagga gctccggggc cttggcgcgg
     5341 tcaccgacct ccgcatcccg accgcgacga tgcgcgcgcg cctccgggcg aagcgggacc
     5401 aggtgtcggg ccaactacga gatctcgagc gggcgtggaa gctcctcgat gccctccaag
     5461 gctgccgctg tcgcggggac tgcgtgctcg tcacgcgtct tctcgaccgc ggggaatcca
     5521 aactggccgt gacgagacgc tcctcgagcg tccgaacgcg gccgaagcga cggaggatga
     5581 atcgatgact agtcgcgcca agctcccgcg tttcgttctg gttctcgcct ggaccctggc
     5641 tacgcgcctc ttcgcgccgt cgtccgggct ggctgcggac gcaatcaaga ctcctgcgcc
     5701 tcagggccac gagtcgccgt tcgcgtgcat catgtccgcc ttgagtcctg ccgagcggaa
     5761 gcagcacttc gaggagtacg ggccgcggct tcgcgccaga cttgagagta ttcgcgagct
     5821 tccgaacgga tacgagttcg cgttccacac cgacaaggac acgtaccagg tgctctcgac
     5881 ctggatgttc caggagcgcc tctgctgtcc cttcttcgac ctcgagcttc gaatcgaccg
     5941 tgaaggcgga tcgacctggc tcaggctcac gggacgggag ggagtgaagg acttcatcca
     6001 ggccgagttc gagccctggt tccggaaggg ctcgtgatcc cgtgcgcggg ggacgcggat
     6061 tgacccccgc ggtggattcc cactaaggtc gagccatgag cgcgccgggg atcgtgcgga
     6121 tcggactggc gtcggtacgc aacgtcccgt cggagagcgg gcgactcgac acgcttcggc
     6181 gtgttctcga tgaggcacgg gcccaaggcg tcgcgatcgt ctgctttccg gagacctacc
     6241 tcttcggtct gaggggacag gatttccccg tcgcgccgcc cgaccaggga cggcaggaat
     6301 cggctctgga ggaagtctcg agggcggcgc gccagacggg cgtggccgtt gtcgttccca
     6361 tggagtggca gacacgcgcc ggcctccaca acgtcgcgtt cgtgatctcg agcgtggggg
     6421 agtgcctggg cttccagccg aagaatcaga tcccggtgga agaggagccg cactacgctc
     6481 ctgaaggcag ccgtcgcatg ttcgaagtgg acggcctcac gttcggagtc acgatctgtc
     6541 acgagggctg gcgctatccc gaggcgacgc ggtgggccgc ggctcgcggc gcccacgtcg
     6601 tgttccatcc gcagctcaca ggcggcgacg acaaaggtgt cacgccttcg ttctggggcg
     6661 acccgaaggc tccgtactac gagaaagcga tgatcacgcg cgccgtcgag aacaccgtct
     6721 acttcgcgag cgtgaactac gccatgcgac atcaggagtc cgcgaccagc ctcatcgatc
     6781 cggacggcgt ctgcgtggcg cacgttccct acggagagga gcgcctcctg gtccacgacg
     6841 tcgatcccgc gcgcgccacg ggcttctgcg cgcgacgcta tcggccggag ctctatcccg
     6901 catgaagccg ctgccgcctc ccagcgccga tctcgcgtcg gccatcctcg gctcgtggtg
     6961 gcttctctct cgcctggatc atgacgacgg agggaaccgg cacgtttgcc cgatcctcgg
     7021 ggcggaaccc ctgggatatc tcagcctcac gccgcggcga ttctcggcgc agttcatgag
     7081 gcgagggcga gagctcgagg ccgaaccgct gcgcgcgccg gcgacgacag cggcggccaa
     7141 caataccggc gccgtcggcg gctacgacgc ctacttcggg acctacgagc tggacgcggg
     7201 tcggggcacg gtggtcgtga cgctggaagc ggcgctcacg cccgcgaaca tcgggaagac
     7261 gttcctgcgc gaaatccgag tcgtcgatgg ccggctgatc atccggctca acacggacgc
     7321 agcggacgga acgccgatct cccgcacgct cacgttcgag aggctcgcct agcgcgccta
     7381 gccgcgcatc atgagcccgg ccacgatgtc ggtccacgcg tgggcggcga tgccgggcgc
     7441 gagactccgc cgccagtggg cgaggaggcc gaagagcacg cccagcccgg tgatcacgat
     7501 cacgttgcgg atcccctggt atccgtgcgt gatgccgaag atcactgcct gtccgaggat
     7561 ggcgggcacg gcatggcccg tgagcgcgcg cagctgggtc tggaagtagc ctcggaacac
     7621 gacttcctcg cagaagcccg ccgacaagga cagaaggacc cagaccactg actcgggcag
     7681 atctcgtggc aggagcgctt ccacggactt ggggtccgga ccgacgacgc ggacgagaag
     7741 cgacgcgacc agagcccacg ccgcccagaa cgcgagcccg attcccacgt ctcggagcac
     7801 atccagaggg cggctccatc gcgcgcccac caggtcccgc acgcgagtgc ccgtgcgtcg
     7861 aagccccacc atcacgtagc gaacgagccc ccactccgcg gcgaggagcg agaggtagag
     7921 gggaagagcc gaggcgcgat tccccgcgct gcgctccgcc ccctgcgact ggacgtaaat
     7981 cccgtacgcg gcgacgccga gcaggatggc tgtgaggatc gccgtgtgtc gaagcgacgc
     8041 tatgagtggt cgacggggct cggtcatcgt ggctttctcc ttgatctggt cctggctagc
     8101 gcgtgaagcg tgcggcgcgt ttccgcgcac caacggcgca cggcgcgcga ccgatgttct
     8161 ccgtagctca aggtgatgag ccagaagggt aacgacgggt gatcgggttg acgctcgagc
     8221 tcgcgtcgaa tgcgggcgaa ctcgcgaagc gccgcggcct ctcgcgcggc gagctcgtcg
     8281 atccaggcga tctccggacc cgcggaggct cggtcgccca ggaagagctt gagcaggagc
     8341 tcgctccgca cgggcgcctg gcgcggcggc tcggttctcc agcacgcgag cgcctcgcgt
     8401 ccctttcgcg tgatctcgta cacctgccgc tcgcggccgc cggtggctcc ccctgggcgg
     8461 cggcggatca gtccggccgc ggcgaggctc ttgagcgcgg gatagagctg gccgtagctc
     8521 tcggaccaga agaatccgac gctcgtcgcg gcctccttcc ggaggtcgta gcccgacatc
     8581 ggtcggatct ggaggaggcc aaggacggca tagggggtgg tgctcgagcc ggcgcgcata
     8641 tatctgaatg atatatcaag cc
//
